LOCUS       DAZKCY010000001        99079 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000001 DAZKCY010000000
VERSION     DAZKCY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 99079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.001"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(305..661)
                     /locus_tag="ACG8XC_000001"
     CDS             complement(305..661)
                     /locus_tag="ACG8XC_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9189737.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /locus_tag="ACG8XC_000002"
     CDS             complement(675..1085)
                     /locus_tag="ACG8XC_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9189738.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1432..3618)
                     /locus_tag="ACG8XC_000003"
     CDS             complement(1432..3618)
                     /locus_tag="ACG8XC_000003"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP9189739.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3836..4519
                     /locus_tag="ACG8XC_000004"
     CDS             3836..4519
                     /locus_tag="ACG8XC_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFP9189740.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4600..5319)
                     /locus_tag="ACG8XC_000005"
     CDS             complement(4600..5319)
                     /locus_tag="ACG8XC_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9189741.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5631..5837)
                     /locus_tag="ACG8XC_000006"
     CDS             complement(5631..5837)
                     /locus_tag="ACG8XC_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189742.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6082..6684
                     /locus_tag="ACG8XC_000007"
     CDS             6082..6684
                     /locus_tag="ACG8XC_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP9189743.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            6825..8105
                     /locus_tag="ACG8XC_000008"
     CDS             6825..8105
                     /locus_tag="ACG8XC_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP9189744.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVLMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8118..8846
                     /locus_tag="ACG8XC_000009"
     CDS             8118..8846
                     /locus_tag="ACG8XC_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9189745.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(8915..10438)
                     /locus_tag="ACG8XC_000010"
     CDS             complement(8915..10438)
                     /locus_tag="ACG8XC_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFP9189746.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10690..11871
                     /locus_tag="ACG8XC_000011"
     CDS             10690..11871
                     /locus_tag="ACG8XC_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9189747.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(11931..13559)
                     /gene="treC"
                     /locus_tag="ACG8XC_000012"
     CDS             complement(11931..13559)
                     /gene="treC"
                     /locus_tag="ACG8XC_000012"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP9189748.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(13956..16667)
                     /locus_tag="ACG8XC_000013"
     CDS             complement(13956..16667)
                     /locus_tag="ACG8XC_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFP9189749.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(16677..17378)
                     /locus_tag="ACG8XC_000014"
     CDS             complement(16677..17378)
                     /locus_tag="ACG8XC_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9189750.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17564..18163
                     /locus_tag="ACG8XC_000015"
     CDS             17564..18163
                     /locus_tag="ACG8XC_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP9189751.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18244..19257
                     /locus_tag="ACG8XC_000016"
     CDS             18244..19257
                     /locus_tag="ACG8XC_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9189752.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19365..20282
                     /gene="rbsK"
                     /locus_tag="ACG8XC_000017"
     CDS             19365..20282
                     /gene="rbsK"
                     /locus_tag="ACG8XC_000017"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP9189753.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20310..20705
                     /gene="rbsD"
                     /locus_tag="ACG8XC_000018"
     CDS             20310..20705
                     /gene="rbsD"
                     /locus_tag="ACG8XC_000018"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP9189754.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            20705..22078
                     /locus_tag="ACG8XC_000019"
     CDS             20705..22078
                     /locus_tag="ACG8XC_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9189755.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22435..24579
                     /locus_tag="ACG8XC_000020"
     CDS             22435..24579
                     /locus_tag="ACG8XC_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9189756.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24631..25671)
                     /locus_tag="ACG8XC_000021"
     CDS             complement(24631..25671)
                     /locus_tag="ACG8XC_000021"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9189757.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(25948..27750)
                     /locus_tag="ACG8XC_000022"
     CDS             complement(25948..27750)
                     /locus_tag="ACG8XC_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9189758.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(27764..28516)
                     /locus_tag="ACG8XC_000023"
     CDS             complement(27764..28516)
                     /locus_tag="ACG8XC_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9189759.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28525..28887)
                     /locus_tag="ACG8XC_000024"
     CDS             complement(28525..28887)
                     /locus_tag="ACG8XC_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9189760.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29063..29935)
                     /locus_tag="ACG8XC_000025"
     CDS             complement(29063..29935)
                     /locus_tag="ACG8XC_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9189761.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(29961..30473)
                     /locus_tag="ACG8XC_000026"
     CDS             complement(29961..30473)
                     /locus_tag="ACG8XC_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFP9189762.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(30611..31402)
                     /locus_tag="ACG8XC_000027"
     CDS             complement(30611..31402)
                     /locus_tag="ACG8XC_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFP9189763.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(31457..32065)
                     /locus_tag="ACG8XC_000028"
     CDS             complement(31457..32065)
                     /locus_tag="ACG8XC_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFP9189764.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32058..32375)
                     /locus_tag="ACG8XC_000029"
     CDS             complement(32058..32375)
                     /locus_tag="ACG8XC_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9189765.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32531..33259)
                     /locus_tag="ACG8XC_000030"
     CDS             complement(32531..33259)
                     /locus_tag="ACG8XC_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP9189766.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33418..34299
                     /locus_tag="ACG8XC_000031"
     CDS             33418..34299
                     /locus_tag="ACG8XC_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9189767.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34536..35579)
                     /locus_tag="ACG8XC_000032"
     CDS             complement(34536..35579)
                     /locus_tag="ACG8XC_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9189768.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35582..36250)
                     /locus_tag="ACG8XC_000033"
     CDS             complement(35582..36250)
                     /locus_tag="ACG8XC_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9189769.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36392..38728
                     /locus_tag="ACG8XC_000034"
     CDS             36392..38728
                     /locus_tag="ACG8XC_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP9189770.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(38843..39154)
                     /locus_tag="ACG8XC_000035"
     CDS             complement(38843..39154)
                     /locus_tag="ACG8XC_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189771.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39335..40810
                     /locus_tag="ACG8XC_000036"
     CDS             39335..40810
                     /locus_tag="ACG8XC_000036"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFP9189772.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            40857..41441
                     /locus_tag="ACG8XC_000037"
     CDS             40857..41441
                     /locus_tag="ACG8XC_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFP9189773.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            41443..41862
                     /locus_tag="ACG8XC_000038"
     CDS             41443..41862
                     /locus_tag="ACG8XC_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFP9189774.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(42143..42427)
                     /locus_tag="ACG8XC_000039"
     CDS             complement(42143..42427)
                     /locus_tag="ACG8XC_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFP9189775.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(42779..43489)
                     /locus_tag="ACG8XC_000040"
     CDS             complement(42779..43489)
                     /locus_tag="ACG8XC_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFP9189776.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43486..44958)
                     /locus_tag="ACG8XC_000041"
     CDS             complement(43486..44958)
                     /locus_tag="ACG8XC_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP9189777.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45096..45569
                     /locus_tag="ACG8XC_000042"
     CDS             45096..45569
                     /locus_tag="ACG8XC_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9189778.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(45722..46273)
                     /locus_tag="ACG8XC_000043"
     CDS             complement(45722..46273)
                     /locus_tag="ACG8XC_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9189779.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46378..46908
                     /locus_tag="ACG8XC_000044"
     CDS             46378..46908
                     /locus_tag="ACG8XC_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFP9189780.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47391..47834)
                     /locus_tag="ACG8XC_000045"
     CDS             complement(47391..47834)
                     /locus_tag="ACG8XC_000045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9189781.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(47850..49010)
                     /locus_tag="ACG8XC_000046"
     CDS             complement(47850..49010)
                     /locus_tag="ACG8XC_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9189782.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49517..51217
                     /locus_tag="ACG8XC_000047"
     CDS             49517..51217
                     /locus_tag="ACG8XC_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189783.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51319..52152)
                     /locus_tag="ACG8XC_000048"
     CDS             complement(51319..52152)
                     /locus_tag="ACG8XC_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9189784.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52456..54621)
                     /locus_tag="ACG8XC_000049"
     CDS             complement(52456..54621)
                     /locus_tag="ACG8XC_000049"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFP9189785.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            54881..57175
                     /locus_tag="ACG8XC_000050"
     CDS             54881..57175
                     /locus_tag="ACG8XC_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFP9189786.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57168..57833
                     /gene="pgmB"
                     /locus_tag="ACG8XC_000051"
     CDS             57168..57833
                     /gene="pgmB"
                     /locus_tag="ACG8XC_000051"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9189787.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            57826..58833
                     /locus_tag="ACG8XC_000052"
     CDS             57826..58833
                     /locus_tag="ACG8XC_000052"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9189788.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            58855..59874
                     /locus_tag="ACG8XC_000053"
     CDS             58855..59874
                     /locus_tag="ACG8XC_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9189789.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60155..61522)
                     /gene="nhaC"
                     /locus_tag="ACG8XC_000054"
     CDS             complement(60155..61522)
                     /gene="nhaC"
                     /locus_tag="ACG8XC_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP9189790.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61632..63053)
                     /gene="tyrP"
                     /locus_tag="ACG8XC_000055"
     CDS             complement(61632..63053)
                     /gene="tyrP"
                     /locus_tag="ACG8XC_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFP9189791.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63296..65173)
                     /gene="tdc"
                     /locus_tag="ACG8XC_000056"
     CDS             complement(63296..65173)
                     /gene="tdc"
                     /locus_tag="ACG8XC_000056"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP9189792.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65464..66720)
                     /gene="tyrS"
                     /locus_tag="ACG8XC_000057"
     CDS             complement(65464..66720)
                     /gene="tyrS"
                     /locus_tag="ACG8XC_000057"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP9189793.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(66832..67037)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            67300..68601
                     /locus_tag="ACG8XC_000058"
     CDS             67300..68601
                     /locus_tag="ACG8XC_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9189794.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(68670..69269)
                     /locus_tag="ACG8XC_000059"
     CDS             complement(68670..69269)
                     /locus_tag="ACG8XC_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9189795.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVI
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            69523..70404
                     /locus_tag="ACG8XC_000060"
     CDS             69523..70404
                     /locus_tag="ACG8XC_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9189796.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            70417..72012
                     /locus_tag="ACG8XC_000061"
     CDS             70417..72012
                     /locus_tag="ACG8XC_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9189797.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASTEETHGRMNQLFATKISRNQLYWTNML
                     LAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGISGFLF
                     GWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLSVFLTL
                     SCISLALFVLGSIGYRRRDLLEG"
     gene            complement(72089..72748)
                     /locus_tag="ACG8XC_000062"
     CDS             complement(72089..72748)
                     /locus_tag="ACG8XC_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9189798.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(73031..73696)
                     /locus_tag="ACG8XC_000063"
     CDS             complement(73031..73696)
                     /locus_tag="ACG8XC_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFP9189799.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(73843..74448)
                     /gene="dhaL"
                     /locus_tag="ACG8XC_000064"
     CDS             complement(73843..74448)
                     /gene="dhaL"
                     /locus_tag="ACG8XC_000064"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP9189800.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(74476..75486)
                     /gene="dhaK"
                     /locus_tag="ACG8XC_000065"
     CDS             complement(74476..75486)
                     /gene="dhaK"
                     /locus_tag="ACG8XC_000065"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP9189801.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(75461..75856)
                     /gene="dhaM"
                     /locus_tag="ACG8XC_000066"
     CDS             complement(75461..75856)
                     /gene="dhaM"
                     /locus_tag="ACG8XC_000066"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP9189802.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            76010..76330
                     /locus_tag="ACG8XC_000067"
     CDS             76010..76330
                     /locus_tag="ACG8XC_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP9189803.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(76442..77299)
                     /locus_tag="ACG8XC_000068"
     CDS             complement(76442..77299)
                     /locus_tag="ACG8XC_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP9189804.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(77407..78710)
                     /locus_tag="ACG8XC_000069"
                     /pseudo
     CDS             complement(77407..78710)
                     /locus_tag="ACG8XC_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(78707..79216)
                     /locus_tag="ACG8XC_000070"
     CDS             complement(78707..79216)
                     /locus_tag="ACG8XC_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189805.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(79391..81526)
                     /locus_tag="ACG8XC_000071"
     CDS             complement(79391..81526)
                     /locus_tag="ACG8XC_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9189806.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(81609..83264)
                     /locus_tag="ACG8XC_000072"
     CDS             complement(81609..83264)
                     /locus_tag="ACG8XC_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9189807.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(83266..83580)
                     /locus_tag="ACG8XC_000073"
     CDS             complement(83266..83580)
                     /locus_tag="ACG8XC_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9189808.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            83750..84010
                     /locus_tag="ACG8XC_000074"
     CDS             83750..84010
                     /locus_tag="ACG8XC_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFP9189809.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            84024..84338
                     /locus_tag="ACG8XC_000075"
     CDS             84024..84338
                     /locus_tag="ACG8XC_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFP9189810.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(85068..85622)
                     /locus_tag="ACG8XC_000076"
     CDS             complement(85068..85622)
                     /locus_tag="ACG8XC_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9189811.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(85748..87676)
                     /locus_tag="ACG8XC_000077"
     CDS             complement(85748..87676)
                     /locus_tag="ACG8XC_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFP9189812.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(87982..88698)
                     /locus_tag="ACG8XC_000078"
     CDS             complement(87982..88698)
                     /locus_tag="ACG8XC_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP9189813.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(88699..90522)
                     /gene="glpO"
                     /locus_tag="ACG8XC_000079"
     CDS             complement(88699..90522)
                     /gene="glpO"
                     /locus_tag="ACG8XC_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFP9189814.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(90536..92032)
                     /gene="glpK"
                     /locus_tag="ACG8XC_000080"
     CDS             complement(90536..92032)
                     /gene="glpK"
                     /locus_tag="ACG8XC_000080"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP9189815.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(92168..93628)
                     /locus_tag="ACG8XC_000081"
     CDS             complement(92168..93628)
                     /locus_tag="ACG8XC_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189816.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(93618..94913)
                     /locus_tag="ACG8XC_000082"
     CDS             complement(93618..94913)
                     /locus_tag="ACG8XC_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9189817.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(95245..95922)
                     /locus_tag="ACG8XC_000083"
     CDS             complement(95245..95922)
                     /locus_tag="ACG8XC_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFP9189818.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(95952..97043)
                     /locus_tag="ACG8XC_000084"
     CDS             complement(95952..97043)
                     /locus_tag="ACG8XC_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFP9189819.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(97144..97578)
                     /locus_tag="ACG8XC_000085"
     CDS             complement(97144..97578)
                     /locus_tag="ACG8XC_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9189820.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(98162..>99079)
                     /locus_tag="ACG8XC_000086"
     CDS             complement(98162..>99079)
                     /locus_tag="ACG8XC_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFP9189821.1"
                     /translation="KVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQGAHHQF
                     IASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFSDVQARG
                     YYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENWEGVQGN
                     LITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDKVEEDGT
                     VQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGYIYVDQD
                     DEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
BASE COUNT        28994 a        20398 c        17516 g        32171 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acgtttgaat
     1201 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1261 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1321 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1381 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1441 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1501 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1561 tagtcttcgt tcttcttcta tcgtcgtcac tggttctgct tttatttcat taaagtcgat
     1621 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1681 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     1741 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     1801 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     1861 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     1921 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     1981 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggctat
     2041 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2101 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2161 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2221 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2281 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2341 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2401 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2461 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2521 ataattagtc aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2581 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2641 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     2701 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     2761 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     2821 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     2881 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     2941 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3001 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3061 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3121 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3181 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3241 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3301 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3361 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3421 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3481 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3541 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3601 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3661 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     3721 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     3781 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     3841 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     3901 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     3961 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4021 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4081 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4141 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4201 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4261 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4321 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4381 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4441 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4501 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4561 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttatttg taaagattcc
     4621 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4681 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     4741 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     4801 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     4861 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     4921 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     4981 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5041 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5101 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5161 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5221 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5281 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5341 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5401 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5461 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5521 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5581 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5641 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     5701 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     5761 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     5821 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     5881 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     5941 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6001 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6061 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6121 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgccttagaa
     6181 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6241 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6301 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6361 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6421 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6481 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6541 tctctcatta ctttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6601 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6661 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     6721 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     6781 taattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     6841 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     6901 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     6961 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7021 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7081 ttttaggaga tcacttaaca agcatcgacg atttttttgt tttgaaatct tcggttattg
     7141 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7201 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7261 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7321 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7381 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7441 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7501 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7561 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7621 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7681 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     7741 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     7801 ttatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     7861 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     7921 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     7981 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8041 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8101 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8161 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8221 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8281 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8341 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8401 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8461 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8521 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8581 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8641 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     8701 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     8761 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     8821 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     8881 agccgatcca aacccttcgg ttcttttttg cctatcagtg caaaagcatt tcctgtgcaa
     8941 tttcacgtcc atccattgta tctggataaa atgttggcca attctccatt tctttcagca
     9001 atacttcttg actttcattt cctgcatata aatgaaaatg ttcggagtca gccggtgcaa
     9061 tcgtgtgatc actgaattga atatatctaa aagcgccact ttccttatct tctgcttcga
     9121 atagatacct tacgccacgg tttcctgcct cgtaggtcaa gatttctttc cctacataac
     9181 gataatttga acgtttcgtt gttccatcct taaacgtaaa tgccatctct ttctcactga
     9241 tcttgattga agcaatatct gtttgatagc ccttcgtata atattcttta tattcttgtg
     9301 ctgtcttatc ctgattcaat ttttctttat attcaaaaac aggatcaaaa gtcccatctt
     9361 ctacatacgt atctactgtt tgccaagttc ctgcccaatc tgaaagagag cgatcttgta
     9421 catcctcgtc ttcaaaataa ccgttgtaga cgctcttttc ttttttgggg gtcaaagtat
     9481 cttctagcgg gttttctgtc tctgtcgttt ttttcaacgc ttcgaggttt gcttccatga
     9541 tagaaagata gttttctcca ttttctattt gttcattcgt caatccttcc aaaggattca
     9601 atacttctaa agatacacct gcttcgtttg ctaacgttct ggcaatacta tctttggcgt
     9661 tttcttcaaa ataaatatag ttgattccat gttctttaac aaagtgtttc agttcagcaa
     9721 ttctcgccgc agacggttct tcactagaag aaagaccagc aatcgatact tgattcaatc
     9781 catattctaa cgctagatag ttgaatgcag catgttgcgt gacaaaagat ctttgttttg
     9841 cttgcttaaa ggtttcttga taaagctgat ggagtgcttg aagtttctcg gtataggctg
     9901 ctgcattttt tgtccatact tcctgttttt ctgggtaagc ttcaatcagc tgatcacgta
     9961 tgtttgctac ctgcttgatt gctaaacttg gagctagcca tacatgtggg tctagctcat
    10021 gtgtatggcc ttcttccccg tgttcatggt catgatcatg tccttcttca cttcccggaa
    10081 gtaaaagcat accttctgtc gcttttatcg tatggacatt gccttcttgt aatgtttttt
    10141 ggattgcggg gacccatgtt tccatgtttt catcattata aacaaagata tctgtatctt
    10201 ggatctttgc catgtcttta gcagaaggtt cataatcgtg ggaatcagtc cctgctggaa
    10261 tcaataactc tacttctcct tcatccccga cgattgcttt tgtaaaatca tacatgggat
    10321 aaaaggtcgt catcacgctc aatttttctt tactgtcctc ggctttccca cttgccccgc
    10381 aagcagttaa catacctgct aaaaccagca tggccaatat acctatcaat cgtttcattt
    10441 atgacactcc tcttctcatc ttctttcatt tgaatgttcc gattcgcttt tcgcaaaccg
    10501 taatagttac gttttagcaa caggatttaa tttatcatat ggttaaaaga actgtcaagc
    10561 gttttcttat tttaattgat gagaaagttt atatgaatga ttgacgatat ttttgcaatc
    10621 aacctctatc attttccttt tatacccgct tttgtctata atgaagttat agataaagga
    10681 ggaaaaagag tgaaaaaaga aacagataca acgaatcgtt tgatccgctt tttaggagga
    10741 aaaacctctt actatgtttt aggaatagtt atcttacttg cgctgatcat ttattttttc
    10801 catcagatct cgtttatatt caagcctgtc ggaatcatta tcgctactat cttgccccca
    10861 ttattgtttg cactgatctt gtattatctt ttggaaccag tcatcaaacg tttgagcaag
    10921 cacctttcga ggaacttgtc agtcattatc gtttatgtga ttattttatt tttattgggg
    10981 cttggaagca tatggcttat tccttttctt gaagaacagg caaagacatt gatcagtagt
    11041 ttgccagatt tgtttcaaga ttttcaagag agtctccgac aatttttgga gaaaacacct
    11101 tttgccgatt catttaatca attttttgct tctattgacg acgtgacgtc tgatatagct
    11161 ggatttattg gaaattattg ggaaagtggc gcacaacgga tcagcaacat tttttcaact
    11221 gttacagcta tttttattac tttatttact ggtccaattg tcgctttttt ccttttgaga
    11281 gatcctcaaa aattttatca gtcagtttta gcgattgtcc caccagcatt tcgtacagat
    11341 tttaaaaatt tgacaaagat tgctaatcaa caattgggtt catttctaaa ggggcaaatc
    11401 attgcttcat ttatccttgg agcagtctac tgggtttgtt tcttactgat cggattagaa
    11461 tttgctagtg ttttagctat ttctgctggg ttgttatgta tcattccata tattgggccg
    11521 tttatcgcct tctttccagg tttgatcatt gcattccaag actctacttt tatggctgtc
    11581 aaatttgtca ttgtctggtt tgccgtacaa cttctccacg gtgacttggt gattcctcga
    11641 gtaatgggtg atcgtttaca gatccatccg attacgatct taatcgtttt acttgtcatg
    11701 ggtgatctaa tgggaattgt gggcgttatt tttggtattc ctatctatac gttagtcaaa
    11761 ttactcgtta cttttatttt ccgtaaattc aaacaacgct acaataaatt ttatggcgat
    11821 aaaggagagt atgaaaactc ttcattttct gaagaagatt attttaaata aaacacaaaa
    11881 cgtcgatctt acgagaatag ctcgtaggaa cgacgttttg tgtttttgtt ttaatttttc
    11941 agcaaagcaa ttgcctggta tggttttagc tccagacttt gatgaagaac agtatcggaa
    12001 taattatcga taagaatatt ggcttctgtg aatccttctg ggatggcgat ccttgtatct
    12061 ttatcataga aatttgttaa tacaagcagc tgctgtcctt caaattggcg aagaaagcca
    12121 taaacttccg gatgatttgc ttcataagca tgataatctc cttgtgcaac gattggatat
    12181 gtttttctca gccgaatcaa tttcttgtaa taggacaaaa tagaatcttc agaagatagt
    12241 tctttttcta cattaatctc ttctagcttg cctccaactt ttaaccaagg tttccctgta
    12301 gtaaatcctg cttgttctgt gtctgaccat tgcatgggtg ttcgagagtt atcgcgagat
    12361 tttgctttga tccgacgaaa cgcttctttt tctgtgaatc cttgttcaag gagtaactta
    12421 taagcattca aactttctac gtcaatatag tcggaaatcg atgaatagtc tgggtcgatc
    12481 atgccgattt cttctcccat ataaatatag ggcgtcccac ggttcaaatg gatcatggtt
    12541 gctagcatct ctgctccttg ttttcgatac tgatcgataa ctacaaatcg attgagcgca
    12601 cgtggctgat catgattatt aaaaaataga gcattccagc cgttaccctc actcatattt
    12661 tctccccatg tatgaaatag tcgctttaat tctgcaaaat caaatggagg agtagaccat
    12721 ttattcccat tttcataatc gactttcaaa tgatggaaat taaagaccat tgataattct
    12781 tctctactag gttctgtata caagatacaa ttttcgatcg tcgtagagct catttcaccg
    12841 actgtgatga tagtggaatc cgtcccaaac gttgcttggt tcaattcttt tagatacgta
    12901 tgggtgattg gtttgtccgt atattcaggt ttgccatcat tttctttatt atttttcaat
    12961 tgttcgtctt taccgataac attgataaca tcgaaacgga atccttttac ccctttttcc
    13021 atccaaaaac gaacaacttt gaatagttcc tctcttactg cttggttccg ccagttcaag
    13081 tcagcttgtg tccgatcata taagtgcaaa taatatttcc ccgtatctcc aaatggcgac
    13141 caagcacttc caccaaattt tgatacccaa tccgttggct cttctcttaa aatgaagaag
    13201 tcctgataat gctggtcccc tgctagtgct tttttgaacc attcatgttc tgtcgaaacg
    13261 tgattcagca ccatatcgag catgatttct atccggtatt tctttgcttc ggctattagc
    13321 tgttcgaatt cctccatcgt accaaagata ggatcgatgg ctgtgtaatc agagatatca
    13381 taaccgttgt ctttttgagg gcttggataa atagggttta gccaaatcat gtcgatgcct
    13441 agatcattca gatacgggag tttttctttg attcctttta aatctccaat tccatcacca
    13501 ttactgtctt tgtaagattt gggatagatt tggtaaatga ctttgtcttt aaatgtcatg
    13561 gatatttacc tccattcaac tgatttatta cctaccacag cttatatata caagttatca
    13621 tttattatta taactgaaca gaaagcgttt gtaaaattta tctttataca ttctaagagg
    13681 tctgttgccc aataaaaaac aagagatcag acatgtattt gtctatcctc ttgtttaaaa
    13741 tagcaaaata ttcttgtttc ttctataaat ggtgttcaaa agcttttatc tacggcaatc
    13801 agtcttctca aatgtaaaac atgaggagct aattttattt gagtattctt tttgctcgat
    13861 gcaggacggc ttttttacaa cctctgattc atggtgttca attagcagct gaccctcgtt
    13921 attagctcaa aacgctgatt tcacaacctc tgtttttatt cattcgattt caacgcatca
    13981 atcatatcga tatgctttaa tttgaaatgg gtaacaatca tcacaataac cgtaaatacg
    14041 atagttaatc ctgctgataa gacataagcc caccatgtga tcactaatgg aaagatgaca
    14101 ttctccatag acgcttgttg caagatgaag tcagtcaata tatatcctac accaaatcct
    14161 ccgatgattc ctaaaagagt aaagatgata ttttctcgaa caatgtacat cgtgacttct
    14221 ttatcaaaaa atccaaggac ttttattgta gataattcgc gtacacgctc ggaaatattg
    14281 atattagtta gattatacaa gacgataaaa gccagcagtc ctgataatac gacaaagatt
    14341 aagacaaccg agtctaagtt cgccattgat tcttcttgcg tttcgatttg tttggacatg
    14401 aaagacgtat ttgtcacagc gtctgtggca agcagtttct cggaaagcat ttcttcttct
    14461 tttgttgtca tcttatctgt acgcagcaaa aatgcattat ccatcatcga ttgatcagta
    14521 actgaagcat aatattcagg ggacatataa ataaagctac ctagatagtt tttcacgatt
    14581 tcttttactt cgactttcca ttctttttga tcttcatcat aaagcgaaat atgatctcct
    14641 gtagataagt caaatagttc tgctactttt tgcgtaatag cgattcctgt tttaggcaag
    14701 gagcgtgggt tctcttcaag atcagttaaa tggataaaac gatccaaatc agctgtttta
    14761 cttggtacga tcactgtcag actttgcgaa gcatgtcctt tttttctcaa ttctacggtt
    14821 tgagtatgaa tagctaggat ctcatttact ttttcttgct tctgcagcat atccgctgct
    14881 ttttgacgac tatcttccgt ggatgaatcc aatgtaacga ttgcttgata gtcaatgatc
    14941 ggtccaaatt gtttggctga aacagaactt agagaatctt tcaatcccac gcctgcaacc
    15001 atcagcccgg cacatccagc aattccgata atcgccataa gcattcttga cttatatcgg
    15061 aagagatttc ggtaactgat tttttgatta aagctaaggc gtgaccacag cggagtgatg
    15121 tattcaagaa aaatgcgttt ccctggcttc ggtgctttcg gttgaagcaa tgcagcaggc
    15181 cgttctctta aatctttgaa taaaacgatc attgcagcgc caaatgcggc aatgaagaat
    15241 gcggcagctg cttgtaaaat cggcggccag tcataaaaaa taagcgcact cccaatatca
    15301 taccgctcgt tagacagctc aaaaatgatc cttggcaaaa gattcgttcc taatatcgtt
    15361 cctaaaacaa tacctatccc tgaggctaga cttgcataga ttgcatattt ttttgcgatt
    15421 tcaactttcg tataacctaa cgcctttaat gtcccgattt ccctgcggtt ttcttctacc
    15481 atacgtgtca ttgtcgtaaa ggtgatcaat gcggcaataa agaaaaagaa aacaggaaaa
    15541 acattggcaa tcgcagcgat acgatcagac agatcaccaa attcttgaaa accgacattt
    15601 tctgaacgtt cattgtatag gtatgatggt ttagttaaat tttcaatttt ttctgcattt
    15661 cgattcaatt cttcctctgc agaggccagt tcacgttctt tctcaactaa ttgcctttct
    15721 tgttcagcaa gttgagcttc ttgttgaggt aatgtttgaa cagcgattgt tcgttgcgct
    15781 tcaggaagct gctggatagt tgctgactgt tgcgctaact gttttttagc atcttctaac
    15841 cgtgttcttg cttgcgttgt ttgtgctttg ccatccgcta ttttttgctg tgccggttct
    15901 aattcttcgt tggccgattt tttcagctct tctaagcgtt cttcgggtcg atcttgtaaa
    15961 tacttttctg ccttttcttg attttgctcc attttatctt tgtaagcttt gctgtacgta
    16021 ttcaaattct ttacgtttgt aaaatcaaga tataagacag aataaatatc tgttttaaaa
    16081 acggattcag gtaaatagac aaaataatcg acgctaccat ttccgacgtt cgcgtatcct
    16141 ctctccaaat tgttgatgaa caatggagaa cggacaaagc ccgtgatggt atactctttt
    16201 tctttcaatt catctgaatc aatctgataa atatcaccga ttttataccc ttcatcttcc
    16261 gctttttcat ccaatacaag ctggtttggt ttttccggga gttttccatc agttaattgt
    16321 agcttgttct gcttgttatt tttgttataa gaaaatattt gaatcacttc gtttttgcca
    16381 gtattggaat aatagatctg ttttcctgtt tctacttttg catctgggat cttttctgct
    16441 tgagcaatat ctttttgtgt caagccacca gtagaaacga tttgcaaatc agaaagtcct
    16501 gctgtaccaa catagtgatc catcgttttt tgcatgacag gtccagcagt tttgacacct
    16561 acaaataaaa gtgttcctag catgacaata aggatgattg caatgaatcg tcctttagat
    16621 tgccgaattt cgcgaaaacc tgctttaaga tatgttcttg ttttcataaa atccccctac
    16681 catacaatct catcaatgga tgaaggatga tcatttaatt caacagacct tactttagca
    16741 tcattaatgt gaatgactct atcagcgatg ggcgctaaag cagaattatg tgtaatgatc
    16801 aaaacggtat tgccgtgttg tctgctagcg ttttgcaata atttcaatac ttgcttgccc
    16861 gtttcaaaat ccagcgcgcc tgtcggttca tcacataaaa gaagtttggg atttttagct
    16921 agtgcacgag caattgaaac cctctgctgt tcgcctcctg ataattggga cggaaagtta
    16981 ttcaatcgat gagctaggcc cacttgcctc aatacctcta caggatcaag cgcatcagga
    17041 gaaacttccg ttgccaattc cacattttct tttgctgtta gattaggaac gagattgtag
    17101 aattggaaaa cgaaccctac atctgttcgt cggtaagcag taagctgttt atctgaaaat
    17161 tgcgcaatat ctgtgtcatc aataatgatc tgaccttcat caggtgtatc catcccacct
    17221 aaaatattaa gaatcgttga ctttcctgct ccacttggac caagtataat cactaattcg
    17281 cctttttcaa ttgaaaaaga aatatcgtca tttgctgtaa tggtcgtttc tcccattttg
    17341 tatcgtttgt attcattttt tacttcgaca tacttcactc tgattcctcc attttcacga
    17401 tatttctcgc tatatctatg catcgaatgc gttttctcac acttatttat tttaccactg
    17461 aatttctcta gactaaagaa aggaatcctt taatttagtt gacaaataaa cgatttatga
    17521 tttacttaca taaaaaacat tgttatttga aagaaggaac atcatgaatg atttaaagga
    17581 aaatatgatt tttgatttat caaaaactgc ttttgtcgcg atcgacatgc aaaaagagat
    17641 cgtaaataac ggtacactct ctccacacac tgcagcatct attctgacaa acaatgattt
    17701 gttagtaaga acattgaaac gtaccgctgc cttgaaagtt ttagtgaacg tagacatctc
    17761 cactttccct tatttgagtc aacaaactga tatgggtgga atgactgctt ctgtacctcc
    17821 agaattcact gatttacttt tgaaggattc tttgaaagat acagataata tgttgactat
    17881 caaaaaatac aatccaagtg ctttttttgg tacttctctt gatcttcaac ttcgccggcg
    17941 tggaatcgaa actatcattt taagcggtgt agcgacaaca aacggtgtat atgctactgc
    18001 attagatgcc tttcaacacg ggtaccatat cgtgcttgct gaagatgctt gcagtgatcg
    18061 tgacaaagaa tcccatcaat tgttcataaa aaagatattt ccgaaaactg cacgagtacg
    18121 ctcaacgaaa caaataatcg aagctatcca acaaagcaaa taagtactgg cacacgtgat
    18181 tttcatttac ttctttttga tttcatggga aaataaccac aggatttaga taggagtatg
    18241 aacatggtga aaaaaaagat cacaatcaaa gaagtcgcta aactttccgg cgtttcaatc
    18301 acaactgtct ccttgatatt aaatggaaat acggagaaat tcagtccgaa aactgttcag
    18361 aaagttttag ctgccaaaga agaattaggc tatcagcctg actattttgc tagacaaatg
    18421 attaccaagg aaacgaaaac aattggtgta ctcgttcctg atatcacgaa tccattcttc
    18481 agtacactga tgcgaggaat tgaggatatc ttgtataagc agaatttcgt cacgatatta
    18541 tgtaatgctg attctgatca tcaaaaagaa atcgaatatc tagccgagct cacacgtcga
    18601 ggtgtggatg gattcattat cgcgacttct gctgtctcta cagatgcaat caacgaaaat
    18661 ttgaaaaaac aaggccggcc cttcattgtt ttggatcaga aaaaatctga aggatttagt
    18721 gatgccgtac gaacagacga ttttcgcggt ggttacctag cgggcatgca cttgcttagt
    18781 ctaggacatc aaacgatcgc ccttgtctat cctgaaaatc caccggaaaa tgtccacgct
    18841 cggattgagg ggtttaaatc tgctcttgat gtttaccaaa tccctcacga ccaactgatc
    18901 ctcttaccta cacagttctc aaaacaaggt ggctaccaga tcacagcaga acttcttgat
    18961 tctgctgcaa ctggtgtgtt tgctttaaat gatgaattag cttttggatt atatcgcgga
    19021 ttagaggaag ctggaaaaag catccctgaa gactacagca ttattggcta tgataatata
    19081 gatatgtgcg agtatatcaa gccgaaactg acaacgatcg cacaaccaat cttcgaattg
    19141 ggacaaacat ctgccaagtt attgttagat cggatccaat tcccagaaaa agaatgggaa
    19201 gaaaaaagat tacctgtacg attagaaaaa agattttcaa ctgcaccctt gaaatagctt
    19261 tcaaggtgtg ctatattttt atcgaactat taaaacgttt tactaaaacg ttttaaaaaa
    19321 taattttccc atcaaaataa atgagatagt tggaggtttg taaaatgaac acggttacag
    19381 taattggaag tattaatatg gacactaccc ttcgtttaac caacatgccc aagccaggcg
    19441 aaacgatgca tgcgcatgag atttttcacg ctggcggagg taaaggtgcc aaccaagcag
    19501 tggcagccaa acgttcaggc gcacggacat cttttattgg aggcgttggg gctgacagcg
    19561 aaggacaaca gttattagat ctactgacaa aagaaaacat cgatacatct gggatagctg
    19621 aaatccaagg cgccacaacc ggacaagcaa tgattatggt agacgcagca ggagaaaata
    19681 gtatcttgat ccatgcaggg gcaaataatg ctttccatga acaagaagtc ctaaaaaaca
    19741 agcaactgat tacaaacagt gactttatta ttgctcagtt tgaaagtagc ttagatgcga
    19801 cgatcctagc tttttctatt gcaaaagacg caggaaaaac aacgatcctg aatcctgcac
    19861 cagctcgtga aacgattcct actgaactac ttgaaaagac agatatcatt ataccaaatg
    19921 aaacagaaac agaaatcatt acaggaatca gagtgacaga tcataacagt ttggtcgcag
    19981 cagccgaaaa actccatgaa ttaggtattg gaacagtaat cattacacta ggcagtgccg
    20041 gcgctttcta tcatacagaa aaagaacacg gtatcgtccc tgcttttaaa gtggatgccg
    20101 ttgacacaac agccgcaggt gatacattta ttggcgcatt aagcagcaca cttcagccag
    20161 accttagtaa cttgaaagaa gcgatccttt atggaaatct agcctcttct gttgctgtgc
    20221 agtcttatgg tgcgcaacct tctatccctt accgcgaagc tttagaacaa tcagcagtct
    20281 aaaattaaca atgagaggaa gaaacaaaca tgaaaaaagg aaaagtaatc aattcagata
    20341 tttcacgagt aatcgcacaa atggggcact ttgataaatt aagtatcggg gatgcaggca
    20401 tgcctgtccc ctttggtact gaaaaaatag atttggctgt ctcaaacggt cttccaagtt
    20461 ttttatctgt tttagaaaac gttttagaag aacttgaggt acaatcgatt gaattggcgg
    20521 aagaaataaa gacaatgaac ccggaaatat tggaaaaaat caaattattg ttaccagata
    20581 caccgattag ctttatccca catgaagaaa tgaaaaaaga gttgaataac tgtcatgcat
    20641 ttattcgtac aggcgaaatg acaccttacg ccaatattat attagtgagt ggagtggtct
    20701 tctaatggaa acgacaatga atcctgaaca gatggtactg tccgagcaga ttcatatgct
    20761 gattgaacag ttgggaggaa aagacaatat tgattctgtc cttccatgtg cgactcggct
    20821 tcgtatcaaa ttgaaaaaac agccagtagc caatgcagaa aaattaaaac agcaaatccc
    20881 ttccattaag tatttcattt tactcgatag atatgcagaa ataatcatgg atcagggatt
    20941 ggctcctgct tacaaggaat tgacgcgtca aatcgatcat tcagctccga aagaaaagct
    21001 aaatccgatc gaaaaactat ttggtttgat atcggaaatc tttacgccga tcattccgct
    21061 gtttgccggt tctggtatat taaaaggtct ggttgtttta gcgactacga ttggctggct
    21121 tagtgaaaca agcggaactt atcatatttt gtctgctgcg tcgaatgccg tcttttattt
    21181 tctgccaatc tttcttgctt tttctgctgc caaaacgttt aaagtgaatg gatatattgc
    21241 ggctgtcatt gctgcagcac tgattgaacc gaatctgacc tctttacttt cagacagtgg
    21301 caaagcagct actgattttc tggggattcc tgtagtactg atgcagtata cgtctactgt
    21361 catgccggcg attttaggta ttttctttta ttcttttgtt gaaaaattcc tcaaaaaata
    21421 tattcgcgaa aatatgcaat tagtatttgt acctttactt tcattagtta tcacggttcc
    21481 tttgacgtta atggtattcg ggcctgcggg tgtttacctt ggcgaaggtt tagctgccgc
    21541 aattacttgg atgatggata ttaacggacc gatcagtggc gcaatcatcg ctggtggttg
    21601 gaatattttg gtaatatttg gtatccaatg ggctgtaaac cctgtcatga tcagtaatat
    21661 ctctgcttat gggttcgacc gaatcgttcc gttgacagga gcagccaatt ttggaatggc
    21721 tggtgcagct ttaggtgtat ttctgcgaag taaacgttca aaaacccgtt cgatctccgg
    21781 ttctgctttt gcttcgattt tacttgcggg agtgaccgaa ccaactgttt acggaattgc
    21841 gatcccattg aaaaaaccat ttgttgctgc ttgcattggt gcagctgctg gtggagctgt
    21901 aatgggattt gcccaagtga aagcgatcgc ttttgtattt ggtagtctga ccacattgcc
    21961 tgcattcatt tccggtactt ttttctggta cttagcagga ctggctgtta gtctagtcgt
    22021 agcaatgatc actactctcg ttagcgggtt tgatgaagac ctaatgtctt atgaatagtt
    22081 aatgaaaaag actgaagtat gagatgttta tcacatattt cagtcttttt ctttttagga
    22141 tcattatttt tataaatttt tgtgatactt tccaaacttt ggtataattt acatatgcat
    22201 aaccattttt atccccactt gtttatcccc aaacaaaggt ggtctatgca ataaaatgaa
    22261 cgtcaaagaa aaacgaatga atgctcgttg cttatttaca aattgtttta tttagaatac
    22321 acagacagat ttatgaaaag aaaaagcttt tcgacacgat tatctgttcc aaaaaatgtc
    22381 taatgaatgc tgaataagac agaaaatcaa tgaaaggaaa aggactagtt tattatgaaa
    22441 aataaaaaaa cgatacgttt gctagctgct acttggctga ttgctccgtc tgttttgtcg
    22501 accaataccg tatttgccgt agataatcaa atagctgatc aaacagtcac aacagatacg
    22561 actacatcca gctcagctac tagtgaaaca acggattctt ttacagaaga cccaactgga
    22621 acaactaact caacaacagg atcgaccgag ccaactgaaa ttgatccggc tgatccatca
    22681 gaaaatgaaa agccagccga gacatattcc cttacaattg ctccttcttt gattggtaag
    22741 atccaactga ctgcttcgac agcggattcc cctgaacagc aacctttgga tattacggaa
    22801 agtggaacgg tagaaggttt attagcggaa acgaaggtaa cttacactat tacgccagct
    22861 gaaggtgaac aaattgcttc agtcgctgtt aatggtgcca aagaagtaaa ttatgatgca
    22921 aagaccttta cagtaggtac gaacaattta tctattactg cagaatttaa agctggaccg
    22981 gatacttcaa cagagaaacc aactgatcct agtgaagaag tccctagtac accagatccg
    23041 gaaacgccaa acaaaccaga tgtgggtaat aacaacaata ataatggttc aaataattca
    23101 aacaataata acaataattc gaacagcggg aattcttcga atcaaacaaa cccatctgga
    23161 tcgaataaca atggaaatac tcagagagaa ccaaaccatc caaacaatgg ttcatcgaat
    23221 ggcggaacga acaatgcttc atctcgtcca aatacagcaa atcaaagcgg tatagagaat
    23281 ccatcttctg cttcttctga ttttgttgta aaatcaccaa ttgatgctgt attgcctaca
    23341 aatacaacga atgtccaaca agcaatcgtt cgagaagcat tcaagcatct tggaaaacca
    23401 tatgtatggg gagcaaaagg tccaaatact tttgactgtt ctggtttgac ttattacgta
    23461 tatatgaaag caaccggtca ttatatcggt ggctggacag gcgaacagca atatgcaggt
    23521 acgcagattc cagtaagtca ggcccaacct ggcgatctcg tcttctgggg accttcttcc
    23581 ggtgttactc accatgtagg tatctatatt gggaacggac aatttatcca tgcgcctcaa
    23641 ccaggagata aagttcgagt aacttctatt tcagatttca cgcctgattt tgctgtccga
    23701 gtaaacttag caggtttacc tgctgcttca ggttcgctga caggcggctc atctatttta
    23761 gatggattag atggctcgtt ccatttcagt caaaaccaaa caactgatca gttcttgaaa
    23821 aagattgcag atgatgcaca ggaaatcggg caaaaagaag gaatctacgc atcagtcatg
    23881 atggctcaag ctatccttga aagtggatct ggaaacagct tattatcaag cgaaccaaat
    23941 cataaccttt ttggtatcaa aggatcatat aaaggaagca gcgtgacctt caatacactt
    24001 gaacaagata gttccggaca aagctaccaa atccgcgcac aattccgtaa atatccttct
    24061 tacaaggaaa gtttggaaga ctatgccgac ttgatcaaaa atggactgac tggcaatcct
    24121 gatttctaca aaccaacttg gaagtcggaa acaaaagact acaaagaagc aaccaaatat
    24181 ttggaaggac gttatgctac agatcgccaa tattcacaaa aattgaacgc gatcatcgaa
    24241 gcgtacgacc tgacaaaata tgatgaaccg aaaaaagaag cagcaacaac tgaagaagca
    24301 aacgctgaaa tcctcaatat gtctaagcga ttcgatgtgc caatcaaatg gacaacagat
    24361 ggcgtgccaa tcacaggatt agaattttca acgataaaac gcgtcaacaa cgcttcttcc
    24421 atcttccgtg tcgcttcaat ctgggatttg tggaacaatt ttacggcaag acagatacca
    24481 gaaacaacta ctcgtacagt taaagctgca actgttcctg tactaacctt gttaagttta
    24541 gacaaatcat tctggcttga tctgccttta aagaaataga ataaattaaa aaggggatgt
    24601 gacactgtca cactcccttt ttaatttatt ctatttcttt atatttattt tgtattccgt
    24661 tacaaactcg cgtacttggt taggttccag agtgacatct ccaaaacttt caagtcgctc
    24721 cgctcctggc agttcttgag tctcaaatgt gataccgcca tgatttacta atttattccc
    24781 gcgcatttcc ggtccatctt ctccaaaatt tgctgtaaaa atcacgattc ctgaagcatc
    24841 tgtttttact tggatcgata ctttttcatc tgggcttgtc aattccgcag catttagtaa
    24901 accagattgt tttttcaaca aaaatggatg atcgatgccg cccactatca ttgattggcg
    24961 aaaatcactg gcaaagacct cacctaattt tttaggacgt tggaagtcaa atgctgtgcc
    25021 acttactgaa actacttctc ctgtaggcgt tgtatcttta ttgagcgtag caaataatcg
    25081 gctattcaac caaagtatat gttcgtcgat tgactgtgct ggatcaccgg ttaaattgaa
    25141 atagacatga tttgtaggat tgaatactgt tttgcggtca ctgatccctt ggaccgtcac
    25201 tcgccatgta tcatcgttcg tcagcgtata ccgtacttct acatctaaag ttccaggaaa
    25261 tccgttttcc atatctgggc tagtctgata gaaaatgacc gatgcttcgt tttcaccatt
    25321 gatgactgta taatgccact gcttggcttc aaagctaggt gcaccaccat gtaaacaatg
    25381 accagtgtcc gggtctgttt ttacttcgaa cgtttcatca tctaatttga atgtgccatt
    25441 cgtaatccgt ccagccatac gtccgattga agcaccgata taagggtctt tttctagata
    25501 ttcttttgct tcatcaaaac ctaagatcaa ctctcgtttt tcattatcga ttattgtttg
    25561 aaaatgaaca acccgtgccc caagatcact gacagacaaa gatgtcccat tttgattcgt
    25621 gatcgtaatc aagtgatagt tttcgccaaa attttgttca ttgatttcca tgttccttgc
    25681 accctctttt ctttcatttg cactgattcc agtatacatg gaaaccgttt tcttaacagc
    25741 ttattccgtg agtaacgaga aaaaatttac tatattctaa taaaagaccg tgaaaaaaag
    25801 gttgtgacaa aaatattgtc acaacctctt tttccgaaca aacggtgttc aaaagcttgc
    25861 atctgcggta ataagcccaa ccaaacgaaa aatatgagga gctatttttg cttggctgtt
    25921 cttattactt gatgcaggac agctttttca caatctcttt ttcaatacga ttccagtata
    25981 taaccatgtc gtcagtacat agtagatgaa atacaatacg aagaagatcg taaacggaac
    26041 ccaaatatct ttgtatggat tttgcatgaa tagaccaaac atttgtaatc cgaataagac
    26101 atggactaca cctaagattc ctggtgctaa gaataacacg cctacttctc tacggatcga
    26161 ttgtttaagc aacccgcgtc gtgttcctat cttttcaagc atttcataac gagcaatgtc
    26221 actcttgctt cctgacaaga tcttgaacat caaacagcta gcaagcatgg tcaagaaagc
    26281 taaacctagg aagaatccca taaattcaaa accagagaat agaccattat acacttcata
    26341 catttcatat ttctggttgc tgtactctac tttttgcaaa gatggattgt tcattttatt
    26401 ttgagcaaca agtgtttcga ttggtttcag gtttgcttgg aagtctttta cctgaactag
    26461 ctgtaaagtg gattgtggca agttcagttg atcaaattct gattgactga ctaccttgat
    26521 ttctttttct tgctgttccg gcaattcata gcttcgaagt tcctcttgta cagtcagttc
    26581 attcattttt ttagcaggaa ctttttcttt cgtgatcttc ggcggtgttc catcttcatg
    26641 ttttatcatg atcaatggtt gagccgcaaa ttcacttgca ttatagtaaa tcgttcccgc
    26701 atcttctttt tgagtataag aagcagaaag tgttggtgaa agttctttga ttttttgctg
    26761 atcttctgcc cgaggattat tcatcactag atcatatcca gaattgtttt ctgtcaagtc
    26821 tgcaatttca tttttaaaac ctaatcctac cgtcaaagcg cccagtgcca aggcaaacag
    26881 catagcgacc attgagagca tttgtgtata ttcacgaata cgaaagttta actgtgacaa
    26941 tgtgaaattg ttcaattttt tcaatgaaat ctgttctgat ttctttagta atgataaaaa
    27001 gaaaatgatc acagaatgga aaatgaaata agttcctaat atgatcgtaa ctaaggctac
    27061 ccctatacca aagacttgat aggtccccac gtgtttcatc atatagtaac cgcagcctag
    27121 aaaagcaatt cccagtacta cttcaaggaa aaacaagaat ttatgctgct tgattcttgt
    27181 tggtgtctga tccgcacgaa tcaatgctaa aatcggtttt ttgacaatcg aaaaagcatt
    27241 gacaacagca gctaataaaa acaaaacagc aaaaaacaaa acagtgatca acaacccgtg
    27301 aatactaaaa ggtgtaaaat gcgaaatatt gagatctaac tgatccacta ataatcggtg
    27361 gacgatactt gtcaatccta caccgacagc tgaacccact aaagtagctg cgatccctac
    27421 taagaatgtt tcagaaaaaa tcatctgtgc gatttttctg ccttttgctc ccagcatcat
    27481 aaacattgca tagtcttttt gccgcatcgt catcaaaaag gaatttgcat ataaaatata
    27541 aacgaatgtg atgatcccta gtaaaacaga accgaatcgg aaaatgataa ctgtagccgc
    27601 aacgatcgta ttacttttca aaaaagcttc gttgcttgcc attgattcaa acatgtaaaa
    27661 aatagcagat gccataacta atcctgagaa taacaccaag tagtctctta aacggctctt
    27721 gatcccagta agtgataatt tccaaagcat agtcaatctc cccctactgt tccaagtttc
    27781 caagaataga tagtatttct cgatagaagg aatcacggct ttgatctcca cgtttcactt
    27841 cttgatggat accgccatcc ttgatgaata aaatacgttg gcaataactt gcagaaaacg
    27901 gatcatgcgt cacaagaaga atcgatacat gatcttttgt attcaattcg tccatcgtat
    27961 ctaaaagatc ccgggcactt ttggaatcca gcgcacctgt aggctcatcc cctaatagaa
    28021 tcgttggttg tgtgatcaat gcacgtgcgg cggcgacccg ttgtttttgt ccaccggaaa
    28081 tctcagaagg atagctttcc aaaatatgag agatggataa acgttctgca attttttgga
    28141 cacgttgctt gatttctttt ggtctgacgc cttgtaaaga aagaggaaca gcgatatttt
    28201 cttgtgctgt caagctttct aataaattga aatcttggaa aataaagcca atctcattcg
    28261 cacgaaaatc tgctagttga tttcctttta atgtggtcac atctttttga ttgattcgga
    28321 tatgcccatc tgttggttta tctaatgtgg ataaaatatt caaaagtgtc gacttaccag
    28381 aaccagaagc gcccatgatc ccaataaatt cgcctttttc tacatcgaag ctgatgccgc
    28441 ttaatgcttg cgtctttttc tcattgtttt ttccgtatac ttttgttaca ttctgtactt
    28501 cgactatttt gttcatttct ttccctaccc tttaattagt ttttccagtg ctgttttcgg
    28561 cacgtctttt ttttcgactt cttcatacga agtgacacct ttatttttgt tccaggtgat
    28621 ttttaaatat gctcctttac gaagcggtcg agtcaattgt cccttgaagt ccagttcttt
    28681 ttccttaccc tctttcgtat agccaggcag gatatatcta tagttgactg tttcttgtcc
    28741 agagtctgat tttgaaactg tttttgtacc atctgtcgta atttgtacat agtattcttc
    28801 tccacccata actacgtaat ctgcgatttt cacccctgca agtaccacaa cgatgaaaac
    28861 aagtagaccg atcagaagtt ttttcacttt ttccccttct ctcttacttt atatactgat
    28921 tatagaaaaa agcgaacaat tctgccttcg aatcaactat cattcttctt tcaattttgt
    28981 cattttcttt aaaacaacta aaaaaagagt tcaagacaat aatccgtcct aaactccttc
    29041 ttcctatctt acaaaaagta tgtcacttac ggtctttcat gtcgcctgta ttgataacag
    29101 gttgcgttcc tttgtctgta atgattgaga caagtaaatt attgatcaat tggactttcc
    29161 gatcatcggt gaaattgatt tcttgtccat cttcgatttg ctctaaggcc atttgagtga
    29221 ttgtgacagc tccttcaaca atggtttgtc gagcagacaa aatggctttt gcttgttgac
    29281 gttggagcat ggcactagcg atttctgttg cataggctaa atggttcaag cgtgtttcga
    29341 ttacttcgac acctgctact gctaaacgct cttgcagctc tttttgcaac tcgtcagaca
    29401 ctgccgtcgt gtttcctcga agcgtcaaat cattctcatt gaatgtatca tacgggtatt
    29461 ggctagcgat atgccgaatg gctgtctcac tttggatttc tacaaaatct tgataatacg
    29521 ctacatcgaa taatgccttt gctgtatcaa cgactttaaa gactactact gctgagattt
    29581 cgatggggtt tccatccaaa tcattgactt ttaaaacaga actattgaaa ttccgaactt
    29641 tcaacgaaac agtcattttt tgtgccaaag gtatcgtaag gaaaaagcca ttttctcgaa
    29701 tcgtacctaa atattgtcca aaaaacaaaa ttacttttgc ttgattagga ctgactacag
    29761 ttgccgaact taacaacaat aacgtgatca accaaagaaa aatacttaaa aaaagtgccc
    29821 aaactttcgc ttgccacata cctaaataga agaaaaacaa tccaactaaa gctaatacga
    29881 ccaatccgac aatccctgca taaccattga tataaaatgc tcttttttct ctcatgctac
    29941 ttcctgcgct cctttatcct tcaataaata tctaatgtta cttttttaat tttttttcga
    30001 atacgcgcca ttacaccttc tagttctaac tctttgtctt cagtgatgta ccaacccctt
    30061 gcctgtgtcc ctccggctgc ctgcggtaaa taatccaatt ttgttcgaac ccagtgcttt
    30121 tcttctgtaa aatagtcttc taccaagacc tctagatcca tccctgctga aagaggcaaa
    30181 tcttcccgaa cagatttaaa gataaatcgt tccttcttcc catcatactt caatattcct
    30241 gtatcgataa tcggtaaaga ataataatcc agctctttct tcaccgtcaa taaatgtttg
    30301 aaaacttcat ggtacacttg ctctaattgg cgaccttctg aattgttcgg gtaattaacc
    30361 atcacttttt ctttgtctgg atcatagaag gtttggctta gttgagtgat ataagtatgt
    30421 aatctttcca tttctgtttt taacttttct ctttgacctt cttctacctt catatactct
    30481 ttcaccctgt tttcttttta gatattttct ttcttatttt actatagtta tgtgcgttaa
    30541 gcgatgaaaa acagcaaaga ttggaaggat ctttgctgtt ttttcttaaa atatccagtt
    30601 gagtacagga tcaatctgtt tcattgtcct cttgatccgt cgtaatattg atcgatgtgt
    30661 ctttagcagt gatctttagc tttttctgtc cttgttctaa caaagtattt gttattggct
    30721 ggtagtcttt ttcaatataa gattcaccag acgtttggat agtatagcca ctgttttcca
    30781 tctcatgcaa agataaattg ccaccgttta ggatgacttt tccttctttg ctgatcaaac
    30841 tgtcttctag atggaagttg ctgttttcac ctgtgacgat caagttatcc acattggcct
    30901 catctaatcg aagtgagcct aattcatctt tcgcagttat tgttcctcct gtaaattcat
    30961 tgaacgatac ccagccatca tgtgtcttag tattcacttt tttgattgat acttcgttga
    31021 tatcaattgc actattattc ccttcgattg tcaattcaga taattcatga tctgcaggga
    31081 caacaatagt gatttgtgca cttgtgtctt ctaaactaat gattgcacct gttgattttt
    31141 tgactgcatt cacagtcaac acgccatttt tctcctttat ttctggcttt tctgtcttat
    31201 cataagtcgt ttttatctca aaatccaatc cgcgtatgac cgaaactgtt tgattttctg
    31261 catggatgac gattttgttt gcatccttga ttttttttgt ctcagtaacc atatcgatca
    31321 accgaggacc attatcccaa gtcagactct tttgagcgcc taatgtataa gttgcacctg
    31381 ctaggaggcc accagtaagc atcagaccaa taccggtaat caaaaaatgt ttcaatctca
    31441 atttatgtgt cctccttcaa acgcccgctg aatgcagatc tctttttgta ataaaacgtc
    31501 gtcccatcca tttaacaaag ttcatcatga cataaaaaag cccttttgtt cctttcagca
    31561 acagtggcga aataaatact gcgcttcctg tggcgaacag accgacaccg ataaagaata
    31621 atccactcag aatcgattgt ccaaacacac tgattcctga gatgatcatg aataggccaa
    31681 cacctaagat actgatacat aaaacataga tactaaaaat gagcgctagt aaagcaacca
    31741 atagtccccc aataaccaat ataaacccca gtgctagtgg aatgaggaca ggagaagcaa
    31801 ataaaccgat aatgattaac caaatcaggc gaatttgtct ttttggattt ttcactaact
    31861 cgttttctgc ttgttcttct tctgaaaagt aactgatttt taattttaag gcaaactgct
    31921 ttggtgtacc atattcatca atgatctgat ctaatgtttt tccagaatct aaaaaatatt
    31981 cctcataata ctggatcaaa tcaaaacgat cgtcttcagg aatttcttga agatgatcca
    32041 caacttcatt caaatattca ttcatttgtg ctctcctcca ataaccggtc taaagaatct
    32101 cgaaaaacaa tccattcatc aataatttct tctaaatgac gacttcccat tggtgtcaac
    32161 gagtaatatc gacggtttct gccttcaaaa ggttcatcat aggtctcaag atatccttct
    32221 tttttcaatc ttctcaaaac cggatatagt gtagattcac tgacagagag actttcttgt
    32281 acgatttttg ttaaggcata cccatacata tcttccttgc ttaatgctgc tagcacagta
    32341 ccatcaagaa gaacagttgg aatctggatg tgcattacgt caccttctat tctcaagtgt
    32401 aatattttat ttcaatctat attatacaac atataatata gtttcaaaat cagtttgtca
    32461 aatacaaaaa aactgactaa aagatttgat cctttttagt cagcaaaaaa tggcttctct
    32521 ttgattttta ttataacagt ggcataatcc aatttgtcca catccagctc agtggcatga
    32581 taacgatcat tgcaggtatc atgtcagcta ctgggaacat tttcacgttg atcattctaa
    32641 aaccggtagc gaccatcaag aatccgccgc aagctttaaa atctaagatc atgtctggtg
    32701 tagtcaatgg ataaataaat tttgccaata agaacaaaat ataaaaaata atgaattggg
    32761 gaattgctat cacagaaacg acatacccta aattacacgc aaaaatagct gctgtgaaaa
    32821 aatcaaggac agatttagaa atcaaaatcg tactgtctcc tgtcatccca gaatctaaac
    32881 ttccatagat ccccgtacca cttgcacaaa atagaacaat gaccgtcact aatgtgctga
    32941 taaattcatc gtgggtcata ccaagctttt cactagggaa gattttagaa ataggtactt
    33001 gcatcagcgc agccccctta tttatcccat tacctaaatg aacaatcaga ccaatcgctg
    33061 ttcctatcac taaagcaaaa ataacagccg gcatattttt catcggcgca atggaataga
    33121 ttcccatccc catcgaacac acaccaaaaa ccatcgtcag ctgtgcttta aaatgttcac
    33181 ttaatttatt tcctactaat ccgccaatta gtccgccaag caaaacggaa cctacattga
    33241 tcaaaactcc agttggcatc aaaaaactcc tctctttttc tacgaagcga aaaaaatcag
    33301 aaaaatttct gtaattattg ctttcgctgc aagaaagatt gtaacagaca gagaagatta
    33361 caccaattca ttttttgtgt taaagtattc tttattggaa taagtaggag gaatatgatg
    33421 gatatccata aattcgaagt ttttcttgat ttagccgaaa caatgaattt caccaaaact
    33481 gctgatcgac aattcaccac ccaaggaaat atttctaaac agattctttc gttagaaaca
    33541 gaattagacg tcaaattatt cgaacgcgcc catagaaaaa tcgaattgac cgaagctgga
    33601 agcttattgc tcccatatgt aaaaaatgtc gtagaacaat accatgctat gcaagaagtg
    33661 cttgaaacct atacgaaaga caacaatctt tcattaaata tcttaacgat accgacaatg
    33721 atcaattata aaggtttttc caaaatcacc gagtttttga aacgccatcc tgaatttaca
    33781 gtccagttga aagaagtgga aagtgtcgaa ttaacttaca atgacgccaa tgaacatagt
    33841 gacacgatcc actttgctag aaatttccag gccccaccaa gttcgattga gtggttgcct
    33901 actgaaacag acgattttgt agcagttcta cctaaaaatc atcctttagc tagtaaaaag
    33961 aaattggatc taagcgaact gaaaaaggag aactttttgc tccttggccc cactacgaac
    34021 ttgtatcaac ctgtactaga tctgtgtcgc gaagctggat ttgaaccaaa aatctcttat
    34081 aaaggagcaa gaatcgactt gatcatcaat atgattgcaa atgacatggg gattgctatt
    34141 gttatggaaa aaacagtcaa gaatctatta aaagaaaata ctgtgattct ccctatttct
    34201 ccaacaacag agagttatct tgcatttact cgtaaagtcg gtgagcattc tttggcttca
    34261 gatacatttt gggagtattt aaatcaagaa gaaaactaag ccctacattt tctgtccatc
    34321 tatccaccca aaaaaagaga gcgacgcatg actttcgctc tctttcttgc atcatgtcct
    34381 tgtttcaatg acacctctaa catttctcgt agttcaataa gaaagcagcg atttttatgc
    34441 caaaagaacc gtaagaacat ttccctaatc acaaaaaaac gactaaaaac ccctaaaaac
    34501 cttcaataga atttttaggg gttttattct ttttcctatt gtttacttaa ccgtatcatt
    34561 gctgtcgttc ctactccttc gatactgcta taatcaatac gtccatcatg cagttctaga
    34621 atttttttaa ccagtgaaag tcctagccca ttcccagaaa tttgacgact tgtatctcct
    34681 tgataaaatt tttcaaacat tcgatctctg gtctcttcat tcattcctac acctgaatcg
    34741 gtcacttcca gtacaatctc tgtggctgtc tcaaatagtt tgatatgaat ctgtccattg
    34801 atttgattgt actttatggc gttatccatg atattcagcc acacttggta caaaaactct
    34861 tcattgcctg tgtaatttac tgcagcaagt tcgatatcca attctagctg ttctttctcc
    34921 catttaggct gcaaaaaaac gattacttct ctgatttgtt catctaatcg atactctttc
    34981 ttttccagtt ggatacgctg gttttccagt ttgttcaatt ttaagatgtt ctctgttaat
    35041 tgtgaaagct gcgtgatact ttcgataatc cgatataaaa atatttggcg ttcttcgtca
    35101 gagagatttg gggcttgtaa caattgaaca tatccttgga ttgttgctag tggtgttttg
    35161 aattcatggg atacactgga cacaaagtca ttcctcagtg tctcgatact attgagttct
    35221 tggaccataa cgttgaaatc tttatacagt tgctgtactt ctgctacttt ctgctgttca
    35281 tccattcgaa tcgaaaaatc tcccgttgct accagactca tatttgtccc tagcgcactg
    35341 attggatgca ggatacgccg cccaacaaaa atcgatattc ctgtacccac gaccatactg
    35401 aaccccgcaa agacgaataa cgggtataac gttggttttc cagccgattc agcaggcgag
    35461 atagaatgtc gagacaataa gaaaataaaa ccgagaaaac acagcagtgt cactaagatc
    35521 gaaatgaaga caatactagc aaaatagatc cataattgtg aaaatagctt ttttttcatc
    35581 tttaaataac cgccttatag cctagtcccc gtactgtgat gatttgaaaa tcttccgtct
    35641 tttcaaaacg ggtccttaaa cgtttgatat gtacatcgat cgttcgttct tctgtattcg
    35701 tatctaatcc ccaaatatca tccatcaact gttggcgagt gaagatcttg ttgggataag
    35761 acagtaattt atacagaagc agaaattctt tttgaggcag aatcaacgaa tgctgatctt
    35821 gtaacactgt ataagcatct agctctaatt ttgtttgtcc gattatcaag ctactgctgt
    35881 aattgatctt tgaacgtcga agcaatgctt ccacacgtag gatcatctca ttgacatcga
    35941 tgggttttgt catataatca tctgctccta atttgaaccc gcgtttctta tcctcaaaat
    36001 cggcctttgc cgtaatcatt agaacaggtg tgtcaaatct ggtgctacgt aatgagtcta
    36061 gcaagtcata gccatccatt ttcggcatca tgatatctgt aatgattaat tctatacgtg
    36121 tcgcttctag ttttttcagc gcatcctctc cattcaatgc ggtgatgact gaaaagcccg
    36181 ctcgttccaa cacagaacgg taaagcaact gtaattgcgg atcatcttct actactaaaa
    36241 tacgattcat tgatctccct ctgttttttg aaaaatttct agattttttc ttgtagttca
    36301 tcttcagtta acatttattt cgtatactac gatcattgtt ctgatatact tattagtata
    36361 ccgaatattt caatacggga gaagggtgat tatgcttcaa ttaaaagata taaaaaaata
    36421 ttataaagtc ggcgaaacca ccaccaaagc attggatggt gtgtctgtcg ctttcagaca
    36481 aaaagaattc gtagcgattt taggtcctag tggttctggt aaaacgacta tgctgaatgt
    36541 gattggtgga ctagataatt atgattccgg tgatatggta atcaacggaa agtctactaa
    36601 agactttaag gacagtgact gggatgccta ccgaaacaac tctatcggtt tcatttttca
    36661 aagttacaac ttgattggtc atctaggcat tattgaaaac gttgaacttg ggatgacatt
    36721 aagtggtgtt tctaaagacg aaaaacgaaa acgggcagaa gatgcattgc accgagtcgg
    36781 cttgacagat catatgcata aaaagccaaa tcagttatct ggtggacaaa tgcagcgagt
    36841 ggccatcgct cgagcactag ccaatgaccc agatatcctt ttgtgcgatg aaccaaccgg
    36901 agcgttagat acagaaacaa gtatccagat catggaattg atccaagagt tatcgaaaga
    36961 aaaattagtg atcatggtca cacataatcc tgaactggcc catcaatacg cagatcggat
    37021 cattgaattt tctgatggta agatcttaac agattcccat cctcacatcg aacgaccaaa
    37081 agatgatcaa tttaacttac gcagaacaaa gatgagtttt tggacagcgt taaagctttc
    37141 cttcaacaat atccgaacaa aaaaaggacg tactttcttg acctcctttg cttccagtat
    37201 tgggatcatc ggaatcgcga tcgttctgtc cctttcttct ggattccaaa aacaaatcga
    37261 taacacgcaa gctgaaacga tggcgaaatt tccgattact atttctaagg taacgactaa
    37321 ccagactcgt gacgatgccg gattgggtgc aagcaaagcg gattatccag acagcaaaac
    37381 gattactgca aaagtcagcg aagaagataa agcacagcac acgaataaga ttgatcaaac
    37441 atacgtcgat tacgtaacag acatcgatcc taacttaagt aataatatcg gatttacccg
    37501 tactacagga atcaatcttt tacgtgatgt aaatggcaaa gtacagcctg tcagcttctc
    37561 gaatcaaaat cctgatacgg aatctctttc tttatctagt acgatgtcag caatgacagg
    37621 tgtgggagtg tcaagtttcc ctacacagct tgacactagc aaagaaaact ttttaaaaga
    37681 caattattct ttacttgccg gaagttatcc agcctcagca acagatgtcg tgctgatcgt
    37741 agacggcaat aataatacta atatcaacgc attgaagaat cttggatttg atgtgaaaga
    37801 agacgaaaaa cttgattttg atgatatcgt tggtacaaca ttcaaattag taaacaacaa
    37861 tacttactat acaaaacttc caactgggaa ttttatccct aatacagatt atgatgcgat
    37921 gtatcagaat gcttctgatg aattgaagat ctcggggatc ctacgcgtga aatcttcttc
    37981 tacaatgaat ctcttatctc caggaatcgc ttatagcgat caattaacga ctcaaatcgt
    38041 caacgaaaac aaagaatcag aaatcgtcaa agctcaaaga gacagtggtg taaatgtctt
    38101 aactactgaa aaagttgacg aaaatgccaa acaaacattg cttagttatt taggtggaga
    38161 cagcttgcct tcaagtatca tgatttatcc gaacaatttt gaagacaaag aaaagatctt
    38221 ggattatcta gatgattaca ataaaggcaa atcagatgaa gataaaatca tttatactga
    38281 tttagctgga acgatgactg aactgactgg cggattgatg gatgctatca cttatgtcct
    38341 tattgctttt gcagggatct ctttagtaac aagtatgatc atgatcagta tcattactta
    38401 tacatctgtg attgaacgga caaaagaaat cggtgtacta aaagctttag gtgcacgtaa
    38461 aaaagacatc actcgcgtat ttgacgcaga aacgtgtatt ttaggtattt cttctgggat
    38521 attaggtgtg tttattgcat ggctagctac gttccctatc aatagcatct tatacaacat
    38581 gacggatcta aaaaatgttg ctcaactaaa tcctgtccat gcgattattc tagtgatcgt
    38641 ctctactata ttgacgatgc ttggcggtca tcttcctgca cgaatggcag cgaaaaaaga
    38701 cgccgctatc gctttgcgtg cagaataagg ttgtgacaac cacatttaga tttgagtcat
    38761 aagagcgctg aaatagtaga aaacagcaag aaacgaggaa gtaatatgtt ccttgtttcg
    38821 cgctgttttt tttaattatt ttttataatt ctggtatact tttctttcct ttgtaccaca
    38881 actcttctgg cgtatacctg tcttttccat ttatgaatac tgttccatct gcttcaatcg
    38941 ttacttcagt cccaatccac atattacgag aaagtgcgag aagatttttt ctttttcctc
    39001 ttcctttttt tccaaattgc aaggcatcgg ttatctcttc tgtccaatga gatttcacgt
    39061 attttacttc tccgttttca aatgtaactt tcaaaatatt cgctatattt tccactttta
    39121 ctgctttagg aaatgactcc ttcatttttt tcactttcct tcctccaaca ctaagtatgc
    39181 gctttctttt agtgataagt atatcatagt ttattctatt atcaaaaaaa cgcatccatt
    39241 ccattgtaga atactttacc gaacaagttg aattgttttc ctctttttca ccttttacta
    39301 tagataggta aagacaacta aggaggaatc tctcatgaca gatcaaccgt atgatttaag
    39361 aaaagtcatc gaagaactaa aaacacttcc aggtcaatat cacgaaacag atattgaaat
    39421 cgatcctgaa gcagaattat ctggtgttta ccgttatatc ggtgctggcg gtacagtaaa
    39481 acgtccgact caagaaggac cagctatgac tttcaacaat atcaaaggtt tccccggcac
    39541 acgtgtgaac atcggaacaa tggctagtcg taaacgtgta ggtgaaattt tgcaccatga
    39601 ttatcgaaca cttggtcgtt tcttgaaaga cgctgtagaa aatccaatca aacctgtgaa
    39661 agtagacaaa gcagacgctc cagcacaaga agtcgttcat ctagctaccg atgcagactt
    39721 cgatattcgc aacattttgc ctgcacctac aaatactcct gaagatgctg gtccatacat
    39781 cacaatgggt gtagtcttag gatcagatcc agaaaaaaca atgagcgatg taacgatcca
    39841 tcgtatggtt ctagaagata aagatacgat cggtatgtac atcatgcctg gcggacgcca
    39901 cattggtcac ttccaaaaac aattcgaagc cttgaataaa ccaatgccta tcactatcaa
    39961 tattggttta gatccagcaa ttgccattgg cacgactttc gaaccaccta ccactccact
    40021 tggctacaat gaactatggt gtgcaggagc tttgcgtaac gaacctattc aaatcgttga
    40081 tggtatagca gtcgatgaag tgggtattgc tcgtgctgaa ttcatcattg aagcagaaat
    40141 catgccaaat gaaacaatgc aagaagatat caacacgaac tcaggaaaag caatgccaga
    40201 atttcctggc tatactgggg atgctaatcc agctgtacaa gtagtgaaag tcaaagcagt
    40261 tactcaccga aaagacaatc caatcatgca aacaacgatt ggtcccagtg aagaacacgt
    40321 atctatggcc ggtattccta cagaagcaag tattcttgcc ttggtcgata aagctattcc
    40381 aggaaaagta gtaaatgtct acaatcctcc tgcaggtggt ggtaaattga tgaccatcat
    40441 gcaaatccgt aaagaaaacg aagcggatga aggtattcag cgtcaagcag ctattcttgc
    40501 tttatcagcc ttcaaagaat tgaagacagt cattttagtc gatgaagatg ttgatatttt
    40561 cgacatgaac gatgtagttt ggactttcaa cactcgtttc caaggagata aagatatcat
    40621 cgtgatccca ggaatgagga accatccatt ggacccatca gaattaccag aatataatcc
    40681 tggctctatt cgtttccgtg ggatgagtgc aaaaactatc tttgacggta cagttccttt
    40741 cgatatgaaa gaacattttg tacgtgcaaa attccaagaa gtagaagatt gggaaaaata
    40801 tttgaaataa tgaatgatct gcaactgttt tgacatcata aaaaggagtg cggaaaatgg
    40861 aacaattggc acaacggaag aaaaaaatcg tgattggtgt aagtggcgct tcaggcacca
    40921 tttatgccat caacctagtg aagaaactaa aagaatatcc gatgatagaa acacacggaa
    40981 tcatcagtcc atgggcaaaa cagaacttaa agctggaatc cgacttatca ctaactgaat
    41041 tcgaaagcct gtttgattac cattattcaa acaaagattt aggtgctgct gtcgccagcg
    41101 gatcattttt gaccgatgga atgatcatcg taccggcaag tatgaaaaca gtcgcaggta
    41161 tctctgttgg tttaggcgac aacctgattg cacgtgctgc tgatgtaaca atgaaggagc
    41221 aacgaaagtt agtcattgtt cctcgtgaaa cgccattaaa tgccattcac ttggaaaact
    41281 tgactaaatt agcacgacta ggtgtgcaaa tcataccacc aattcctgct ttttataatc
    41341 agcctcaaac gatccaagat cttgttaatc accatacaat gaaacttttg gatcaatttg
    41401 gaattgtaaa tgagcattca aaacgttggc agggtgatta atatggaaaa agaaacgata
    41461 gttgaagtaa ccaaactagg tttcacgatc caagctgctg ttaaagtaat cggtaaagac
    41521 ttattgatca ctttaacagg cggagataca ccacatatcg gaacagtcac tacattttct
    41581 cgtgatacag agatgcaaac tgtacgtttc cctagccacg atggtcggtt ccataaagac
    41641 gatgtcttag ccgaaaaaat agctgtgatc atcaaaactg ccgttccggg aagttgtacg
    41701 atcacttccg gtatccatgt tgaccatatc tccaatgcac aaatcaacgc ctcttttccg
    41761 atgtcagaag agttaggtta caagatttta gattggttgg aaaaaataga tttccatatc
    41821 aaagaaccag tttattataa agatggtgaa aaaacattat gaaagaagag gttgtgaaat
    41881 cagcgttatg acctgagtat gagagaaaaa aatggaaaaa tagcttctca tattttgccg
    41941 tttttgagct aataccgagg gacagctgat tgaacgccgt ttatcaagag gttgtgataa
    42001 agccgcttcg tttcgagtag taagaaaaat tttgtaaaca tgggtttcca catttttgca
    42061 taaatttgac ttactaccga gaaactggct tttgaacacc gtttatcaag aggttgtgac
    42121 acgtcttttg tcacaacctc ttttattttt gcttcactgt aactgctaca atatcaccga
    42181 tttcttttcc gatgttttta cgaatatctt ttcgtagtcc aaggatgtag cagactgaac
    42241 catctggatt tttcacgccc atattcacga tactgccttc atacggcaca ttgtcaaaag
    42301 ttgcttggac tttaactctt ccagcaccga attcttttct tatatcatac ggaaaaataa
    42361 tatatgcccc gcccttgtcg acttcggaag catagatttt tgattcaaaa gaataaattt
    42421 ccctcatttt atttctccta ttttttacat ctagtatagc atagcaaaaa aaagaaggaa
    42481 ctgtgacaaa agtcttgtcg cagttccttt tttcgaataa acggtgttct cctgccaact
    42541 cctcggtcac aagtcacaat agtcaacaaa catgagaagc tgttttttga aattgctcct
    42601 tgtgactcag agttaaacgg caggctcaca acctcttcct taaacggtgt tcaaaagctt
    42661 gcatctacgg cactaagccc aatcagatga aaaatatgga gtgctatttt tatctgattg
    42721 ttcttactgc ttgatgcaga gcagcttttt cacaacctcc tacatggtgt tcaaaatatt
    42781 attcgccttt tgcttctttc actaattttg cgaatgcttg ttgttggaat tcagcagcta
    42841 aggctttcgt acgttgttct tgctctgctt tgctcatttt tggattttca gtcattgctt
    42901 ttaccatacc gtcttgaaca taagcttttg cttcactttc catcgtactt tgctgtttct
    42961 tcactaattc agctaacttg gctgcttggt ccgtacttaa ttctacccat gtctctggga
    43021 ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt atcagcaata ttgaaccaga
    43081 atttatacat gtcgtttttg tattcccaat aagtagtttt cgtcactttt tcagcatcgc
    43141 cgtctgtctt ttctacttta tttgtttcgt tatccgtacc agttgctttt ggtttttctt
    43201 gtttttcatt tgttcggtaa agatacactt tttcttttcc attccctaaa gctttataca
    43261 aaagcatatc tgctccttgg gaatcagcac tggaaacaat tttttctgtt tttgtttccg
    43321 ttactttttc cattccaaag tgattggaca aattagcgac gatcaaactt agtgaagcga
    43381 cgaagattag tccgaaaatc aaagataaaa aggtttgcca tgttttttta gcgaacacgt
    43441 tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc aataatcatg cattatcccc
    43501 cgctttcatt agaacaggtt cttttttctt caataaaaag gcaataataa agccaagaat
    43561 cccaaagatg atcgctacga taaacgcggc atggtaacct gacagtgtgg cattgattgc
    43621 ctgatcttta taagctaatg gcgtattatc caatacagat ttgcttggga tatttgcttt
    43681 tgtcacattc gtcaacacac tgatcaaaat agctgtacca atcgagcttg cgatttgacg
    43741 gaatgtatta tttaccgctg tcccatgact gatcaagtta tttggcaatg cattcatacc
    43801 agaagtcgtt accggcatca tgaccatgga gatcccaaac atacggatcg cataaaggat
    43861 cacgatatat aaaactggag tcgtttttgt caagaaagca aatggtgcag ttgccgctgt
    43921 caaaatgaac atccctgtga tggctaaacg tttcgcgccg tatttatcga aaatcgcacc
    43981 cgtcacaggc atcatagccc ccatgatcaa tgcacccggc aacaacatca agccagaatg
    44041 gaatgctgat tctccacgaa tattttgaat atacaatggt acgaccattt ctgcccctac
    44101 catcgccata tttgttacac cagctaaaac agctgcaatc gtgaatgtag atgatttgaa
    44161 tacacgaagt tccaagaatg gatgttccaa atgtaattga cgccatgaga acaaggcgat
    44221 gaaaatcaca ccaacaacta agaaaccaat tactttcaag cttccccagc cgtcatctcc
    44281 tacactagaa aatccgtaaa gtaaactacc gaatccaatc gttgataaaa tagcggataa
    44341 aatatcaatg ctaggatttg acaatggaat aacacttttc attaggaaga atgctaaaac
    44401 caagacaaga acaacgattg gcagtaccat accaaacaag tcacgccaat ggtagttatc
    44461 aatgatataa ccagataatg tagggcctaa agcaggagca agtccaataa cgatccctga
    44521 catccccatt gctgatcctc ttttttctgg cgggaaaata gatagcatga tattttgcaa
    44581 taatggcatc gatactccca cgccgcaagc ttggatcaag cgaccgatta gtaatgtact
    44641 aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa aagatcgtca tggcagaaag
    44701 atataaattt cttgaactga atttattgat cagccatgct gttattggga tcatgatccc
    44761 gttgactagc aagaaaccag tcgttaacca ttgcacatca gacgctgaaa tatcgaaagc
    44821 tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga ttcaaaacgg tacaaaatgt
    44881 accaattaat aagacaacaa ctaaaagtgt ccgattatat ggttttccgt aaatatctac
    44941 gggttggttg ttactcatat ttctccttct ttctgaattt tttctgaaac tactagaata
    45001 ctttagtcct aaattcgcta tttgtcaacc tacttgacac ataagttaat acggatataa
    45061 ttaagggaag caagaggaca aaaggagtga caaaaatggt tggggcttcc cttaaacgtt
    45121 tgttggaaaa tgatcaattg gttgttggca tcagtgaatt aagtgatatc gtaggtgtgt
    45181 ctccacgtca attaaggtat tgggaacaaa agggattcat tcgttcgatc gaagctgatg
    45241 cgaattgtcc tagaaaatat cgtttaccta cagtcataaa agtagaactg atcaaaaaat
    45301 atttagatga cggatttact ttgtcaaaag ctgcagaaaa ggcagaaatc cgccaaaaga
    45361 aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa agatatcgaa ttgatcgacg
    45421 aacgctatac gattttacaa atcggtaact tcaaagaaga gaatgctaag atgtacatca
    45481 ttcatgacaa cgagacagac gaattaactt acaagctcct tcctgtcgat gaaaaagctg
    45541 actacgaaca gttgttaaaa gaactctaaa cgccactaat ctcctaagta ccaagctttt
    45601 taataagtag atgctacata aatacttgaa aaaaggcctg tttgaacata agaatatccg
    45661 aacggtgatc tgcgtttctt tcatatagaa aggaattctc catcactgtt cggatatttt
    45721 tttatttttt tcggttactt tcttctaata catctgttag ccaatttgca tattctgttt
    45781 cccgattgat cgcatgcgct aaaatcaggt agtgtccgta attttcatgt ttttcttttt
    45841 cagtaggaaa gatcgttttt tttcgctcta atagatgaag tcgtttttct tcatgaaggt
    45901 cacgctgctg ctgaataatt tctactaatg ccggatcatt cacgtctttg acgaaataca
    45961 atttcagaac aaattcgtct cgattaaccg gtagttcatt ggttggtgta tgaatccact
    46021 ctgccaattg ttccttccct tcttctgtta attcataaac tttcttttcc aatttttcac
    46081 ctgtgatctc tacttgatgc tgaatgattc cttgctcttc catttttgca agttcaggat
    46141 aaatctgact atgttttgct tgccaaaatt ctcctatttc atttttgaac gattgattca
    46201 attcatatcc tgtcatcttc ttcttactta ataaccctaa caaaatatat cgaagtgtat
    46261 tttttcttgc catcagtttt ccctccttga caaatcttta aatccattct atcataaatg
    46321 agtaatcgaa aacatgtagt ttattacatg ttttaattta taaggagatg cttaaacatg
    46381 aaagaattta aagaattgga agattttatt ggaactcact ttatctatac gtatgacaat
    46441 ggttgggaat atgaatggta tgccaaaaat gaccatactg ttgattaccg tatccatggc
    46501 ggaatggtca aaggccgttg ggtaaaagac caagaagctg atatcgttaa attgacggac
    46561 ggcgtattca aaatcacttg gacagagcca acagggacag acgtggcatt ggactttatg
    46621 ccaaatgaaa agaaacttca cggaatgatc tttttcccta aatgggttga agaacatcca
    46681 gaaatcactg ttactttcca aaacgaacat atcgcagaaa tggaagcagc tcgtgaaaaa
    46741 tatgaaactt atccaaaatt acttgttcct gaatttgcta cgatcactta tatgggtgat
    46801 gcaggtatag acaatgaaga tgttattagc gaagaaccat acgatggaat gccagatgat
    46861 atccgtagcg gaaaatactt cgatgaaaat tataaacgag taaaataagg ttgtgaaaga
    46921 gaggttgtga aatcagcgtt ctgacctgag tattagagaa agaaacggaa aaatagttcc
    46981 tcatgttttg ccgttttttg agctaatacc gagggtcagc tgattgaaca ccgtttatca
    47041 agaggttgtg aaatcagcgt tatgctttga gtaataagaa cgctagttag aaaatagctt
    47101 ttcatatttt ctaggctagt gggcttatta ccgagaagca gccgtttgaa caccgtttat
    47161 caagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaatg gaaaaatagt
    47221 tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta
    47281 tcaaagattg taacaactgc atattcacac agttaaatta gtgacttcaa aatgaaataa
    47341 tgctcccagc aaaaaattag gctgggagca ttatttcgtt attctgttca tcaaaaatca
    47401 gcgttttgat tccatttcat cacaagttcc tcctcattta ctgcttcatc tttttgctgt
    47461 gtttgaattg taaaaccttg tgaaagatag aaatgaatag ctgattgatt tttttgatag
    47521 actgtcaaag tcagtgtttt attggtttct tttatttttt tcattagttg cgtaccgatt
    47581 ctttgttgcc ggtattccct tttcacaaag attcctgcga taaactgatc aatcacccca
    47641 gcaaaaccaa taatttcttg atctatttct gctaagtaaa tcactgcatc tggtaaagct
    47701 ttcctaacag attctacttg attctcccaa taagttgatg gaatgaattc atgagaatcc
    47761 aagttagcat gcagccaaat cgatagaaca gattccagtt ctttggcaga caattttgtt
    47821 gttgtgcgga tcattttttc tctccttact tattcattga ttttaaaata aaaaccattt
    47881 ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt atatcttctc ttgttttact
    47941 tttctaatac caaaagaaac agcaacttta ggatcatcag ctgaaacgat tcgataagtt
    48001 ccaccaggca attgatccag cttgactttc ccatgatggt cgctttgtac tgaaaacggc
    48061 acaccatttc tagtaagtgt ttttttctta tgtttcaaaa tgaatgaact accatttacc
    48121 ggtttccctg acgaatcact aataataaat gaaaaatcaa gcactttttt acttaatcta
    48181 tactgataga ttagtcgtcc gataaacact aacagtatga ctacagctat tatcgttcct
    48241 gcaagaataa accattgccg catggttctt tcctgatttc cttttccttc ttcttttttt
    48301 accgattcgt tataaggaat ccgatgtcct gtcactagca aacgatgcgt gttgatcata
    48361 tacggcgtac atgtaagcaa ggtaactaag tcttctcctt ctgaaatcgt tagaacagac
    48421 gtgtcatctg gcagtaccac ttgtatattt ttaacttgat aagccaattt ctcccccaaa
    48481 acagtcaaaa taaacgtgtc tccgttttct aaacgatcta attcggtaaa taaccgtcga
    48541 ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg aatttccacc aagcgggaaa
    48601 gaggttcctt gcaagacgcc tgctccattt tcaaggatcc taccattgag ccgatcataa
    48661 agtgaaatat ttaattggat cttaggtata ttgatacttc cgatcagcca ttcctctaat
    48721 tggctggatg ttgtactttc atttctgttc ttttcattga aaggatcagt accaggattg
    48781 aatccttggt cagttaatcg ttgttttttc tcctccattt tctttcgctg cacttcaatt
    48841 tcagctgcgg tccgtttttg tacttgttcg atacgttttt gatcaataaa actatttaaa
    48901 gaatccacat aaaatggata taaagcgatc agtaacccta cgacgataca tattaataaa
    48961 aacaaacgaa acaaaagtcg acgagactgt ttttgcttac tctgcttcac atgtgtcctc
    49021 ccttgcaagt ttttttgttc tttcttttat tatatgggtt caggaaaaaa atagaaaatg
    49081 aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt aataagtaca gcccaaaaaa
    49141 atagctactc atattttttg ttgggttggg cttattaccg cagatgcaag cttttgaaca
    49201 ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg agtaataaga gcgctagtca
    49261 gaaaacagct tctcatattt tctaggctag tgagcttatt accgagaagc agctgattga
    49321 acaccgttta ccgagaggtc atgaaaaagc tactttgttt cgagtattaa gaaaaattct
    49381 gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc ctctctcctt agaactttca
    49441 cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa ttttctaatt tcttgcctac
    49501 ctctcgcata ggatatatga tgaatttatc taaagtttgc aaaggagttc gacaattgaa
    49561 aaaatatggc ccatttttag caattttttt cattgctttg gcatttctca tgcctcattt
    49621 attcacacat aaactaattt tgggtgctga tgccccattc cattacaatc ggttctatga
    49681 tgccgcgcaa caaattaaat atggggattt ccattatttt ccttcccttt atggattcca
    49741 gcaaagtggc aggatcgtga atgccgttta cggcccattt ttcgcttatt tacaaggatt
    49801 gatcgtgttg cttgcaggta gctggtttca ttatcagatt ctttcaaatt tcattcttta
    49861 tcttatttca ggattttcta tgctgatttt actgagatat gtcaatgttc gaaattgggt
    49921 aagtgtacca gtagccgttt tattcatgag tacgtattct attcaatact ggtctatcaa
    49981 ccaaggattc actagctggg ggacttgttt ttatccttta tgtatgatcc ctattgctga
    50041 tttcgtcctt cataaaaagt tccctgttat caaagtcgct gtctcagtgg gacttatgac
    50101 acagatccat ttactgtctt cgatcatgtt agctttgatg tatatccctt tttatcttta
    50161 ttactttttt aaccagactg aaaaaggaaa agcactgctt caactgatta agtcggtctt
    50221 gctttatttt gttctgacag caaatatttg ggcaggatta gctattgttt acagtgggaa
    50281 taagattctt cctccctttg ttaacagaga aatgagtcaa aaggcgatag atctggatgg
    50341 cagttattgg ctccattacc ctaaggcctt tcctatattg atattatcag gttttttgct
    50401 ttttattttc tttcaacaaa agaacacctt attccaaaaa atcgtgttgt acacaagtct
    50461 tgttttcttt tttctttcta ctagcttgat tccatggacg acattgatcg aaaaaaatgt
    50521 gccttttatt agtttgatcc agtttccatt tcgatttttc gtgccattta cgctattgct
    50581 tctactttac cttgcccttt cactgaacca gtggtctgaa caaaagtggt tccgtctttt
    50641 aagtatattt ggcattctta ttttttcagc acagacgatc cagaacttat accagcatct
    50701 ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact tatctattcg atacgccgga
    50761 agaagcaagg aaaacattct tcagcaaaga tatgtctacc agcttgttcg ttttccaaaa
    50821 aaccacacct gactatctgc caatctataa ggagacaaaa gacaataaat acgaccggta
    50881 taacgaagaa atactagaaa acgaaacaac ttttataaaa acacatccag gcggtctttt
    50941 gacaatcaag tggaaaaaca ctggggacaa aactgttcat atccctgtta tcgtctacga
    51001 tcggactgtg ctacagcaaa atggaaaaac attgactgat tatgaagtaa cagatatcgg
    51061 gaccccaatc gtgaagcaga aaaaaggaat caacgagctc acgcttcatt atcaaacgcc
    51121 aatctatttc tatttcattc tttctcttac tttgattggc tggtttacct tgctatgttt
    51181 atttatttat catagataca agcttttacg tgcttaggca aaagggataa atacatccga
    51241 tcaaaaaact ctccaaacgg tataatcgtt tggagagttt tattttaatc agttttttta
    51301 tgttatatgt tatatttttt attcgttcac atctaattcg atttccaccc cgtagtgatc
    51361 actgatgatt ggactatcga tcccatcaaa aatccgttga tattttttaa cctgcattcc
    51421 ttcagggacg aaaataaaat ctatacgcaa tttttctgta ttttgttccc aaccatctat
    51481 tttcttttcc acagtagctg cttttccttt ttcattcgcg acaacatacg aatcttgaag
    51541 aagaaggcaa ctttctctta cagtataata accttgagag tctactggat tattgaaatc
    51601 acctaggaaa aaaagtggta cgcgagtttc caaaaagtag ttttccaagt ttttccactc
    51661 aaaataaaag ccactttttt tatctgtcca ccaagaaaag tggcagctcg agacaaaaat
    51721 gtcctgatca tctatctttg ttttcccaat caagatcttg cgtgtctgat aatcttcttt
    51781 cttttgagat tgggagacgg taaggacctg actttccaat gtttttttcg taagcagtgc
    51841 atttccttct tcatatttat cataaccgat gtggctcatt tcccaactcc aaaaatactc
    51901 ttttccgtgt tcctttaaaa gctgaactaa acggtaggca aaattatctt tacggatagg
    51961 tacttgattc tttacctgac agaacttcat tagcttcgca ggttctactt cttcagattc
    52021 cagtaattgg ttgatttctt gtaaagcaat cacttcataa tcttctaaca ggatcttatc
    52081 tgctaattct tgcaattttt tctctggttc tggctcaagc cagctatgcg tgttgagtgt
    52141 catgaatttc attggttttc tccttgtctt catttcgaaa caaaagggtt gtgacaaaat
    52201 ttttgtcaca acctctccag caaatagtgt tctcctgcta actcctcagt aataagctca
    52261 atggaccagg agctattttt tgtttggctg tttttatcac ttggtgcagg gcagcttttt
    52321 cacgacctct ccactaaaca gtattcaaac tgctgcttct cggtaataag cccaaccaaa
    52381 cgaaaaatag gaggagctat ttttgtttgg ctgttcttat gacttgatgc aggacagctt
    52441 tttcacaacc tctccttaca ttttaatgga gccgatcact ttaccagctg tcactgtacc
    52501 taattgcttg atcactactt cttctacttt atcgtttgta aatacgatca tgatcgttgg
    52561 atctttttct tcgttttgga tcacatcaag atccatctca gctaatttcg ttcctgttcc
    52621 tacttcttgg ccttctttga cagcaatctc aaaagctggc tgattcattt ggactgtatc
    52681 gattcccata tggatcaaaa cttcgatacc atctttcgtt tccagcccaa ttgcatgttt
    52741 gctagggaaa atacttgtca cttttccagc gatcggcgca acaacttctc ttgatgttgg
    52801 aataacagca aagccgtcac ccatcatttt ttgtgagaat accggatctt ttacttcaga
    52861 taaaggaatc acttctccag aagctgcgga aactagttct tgttctatac ctaagtattg
    52921 cttactacct gcttgtccat ttcctgtaga aaccatttct ggctctggaa tctctgctcc
    52981 tgattgcagc agatcttcga tatctgattt caagacatct gcttttggtc cataaactgc
    53041 ttggactcca ttgtctttga cgatcaatcc catcgcccct gctcgtttcc aggcttcttc
    53101 agtccctact ttttctcgat catttacact tacgcgtaac cgagtcatac aagcatcgac
    53161 atctttgata ttttcttttc ctcccagcaa atgaatgatc tggaccactt gctgatctgc
    53221 tgttcctgtt ccgctgttag atacaacggt ttcttcactg tcattgtcgt aatttccgtt
    53281 acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa tttgctaaga aatacgtaac
    53341 aacaccaaaa gcgatcacac acaacacgaa gttgataaga tctccaccta gacccgcttt
    53401 gatagctaac ggtgtccgag tcagcaattc gatattccca aatgaatgga cccgcaatgg
    53461 caagatatct gccatcgcaa atgcagcccc ttggatcact gaataaatga catataatgg
    53521 cacagcagca aacataaaca tgaactccaa tggttctgtt acacctgtta gaaagacagc
    53581 caacgctgct gaaaaataca ttgatttata tcttgctttc ttatcaggat cgacattacg
    53641 atacatcgca aaagccattc ccatcaagat acctgaagaa ccaatcattt gtcctacttt
    53701 gaaacgagcc ggcgtccagt tatgcaacac aaactcatat tgcgacatat cccctgcacc
    53761 tttaagattc actaaatcag tggcccaagc caaccataac ggatcttgcc caaatacttg
    53821 tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg ccaagctgtg tatagttgat
    53881 aggaatcgtc agcatatgat gcaatccaaa tggcaatagt aaacgttcta atgttccaaa
    53941 taaaaacggt gctagaatcg gtgcgctttc ttgtgattct gcaatccata aaccaaaatt
    54001 attgattcca gcctgtacgt ttggccacac cacagcaaga atcaatgcca cgattgttga
    54061 ccataagata acaacaaacg gaacaaagcg ctttccattg aaaaatgaca aagcatccgg
    54121 caattttcta taattatagt acttattata agccattgcc ccgacaaatc cagcgatgat
    54181 ccctacgaat actcccatat tcaaagctgg tgcttccaat acacttgtaa aaaatccttt
    54241 aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca tcttcattcg caagcatgtc
    54301 acttgtgaca ccaaagatag ccccagtgat ccgattgatt aaaataaatg agatcccagc
    54361 tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct cctattgcta aagcaaataa
    54421 aatatgaaga ttaccaataa ttgcccaacc aatattttca attactccgc ccgttgtgac
    54481 aagcggcgtc caatcaggat tgatcaaggg aattgatttc cctatactga tcatcaaccc
    54541 agctgccggc ataactgcta ccacaaccat taaagccttt ccaaattttt gccaaaactc
    54601 aaaactaaat atctttttca ttccgtttag ctcctttacg caatcggttt cgttaaacgt
    54661 attatagcgt gcgttttctt ttttgtaaat gctttatttt ttatttttct gtctatacca
    54721 ttctataaaa cctataatac gtgtgttccc ttctataatt ttctcctaaa aatttatttt
    54781 tttaagcttt tttctatgaa atattttctt ttgtctattg acttgtgcaa tcggttgcgt
    54841 ttattatgaa cgtataaaac agctcattag gaggagaaaa atgaaacaga tcaaacgact
    54901 atttgctatt gatccatgga aaatcagcac gacacaatta gataaaaaaa atcttcgact
    54961 gcaagaatct ttaacaagta ttggaaacgg ttatatgggg atgcgcggga actttgaaga
    55021 aacttactca ggggaccacc atcaaggtac ttatttggcg ggtgtctggt atcccgataa
    55081 gacacgtgtc ggctggtgga aaaatggtta tccagaatac tttggaaaag tgatcaacgc
    55141 cttgaacttt atcgcgatag atatttcgat caacgatcag ctgatcgact tggcttcttt
    55201 ggaaccagaa gacttttact gggaattaga tatgaaaaat ggtgtattat ctcgtacatt
    55261 cacaatcact acttctacta ataaagtacg catttccttc gaacgttttc tgagcattgt
    55321 caaaaaagaa tcagcttata tctatttaac tatagaaatg cttgaaggta gcggaacagt
    55381 caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa gacagcaact atgatgaaca
    55441 tttttggaat gagattgatc gtggaactat agaagaaatc ggctatttga cgactaaaac
    55501 tattccaaac aactttggga ttgaacaatt tactgttact gcagcaatgc gccatttcgt
    55561 taatggaaaa tcagtcatcc ctgactatca cgaagatact ttagcattac atggttgctt
    55621 gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa aaagaaatta tcgttcttac
    55681 tagtagagat attccagaag cgcagcaagc agaaaaagct gtctcagaat taacgaacca
    55741 gacaaacaat tatatccaag caaaagaaga gcaaacagcg gcttgggcag aacgatggaa
    55801 gcttgccgat gtggtcatcg aaggagacga agaagcacaa caaggtattc gattcaattt
    55861 gttccaacta ttctctactt actacggcga agatgaacga ttgaatatcg gccccaaagg
    55921 attcacagga gaaaaatacg gcggagccac ttactgggat acagaagcgt atgctgtccc
    55981 tctttatctt gctttagcca aacctgaagt aactaagaat ctgctgaaat atcggcataa
    56041 tcaactgccg caagcaatcc acaacgctca gcaacaagga ttaaaaggcg cgctttatcc
    56101 tatggtcacc ttcactggtg tcgaatgcca taacgagtgg gaaatcacat tcgaggagat
    56161 tcaccgaaat ggcgcgattg cctatgcaat ctacaattat acgaattaca ccggcgatac
    56221 ggaatatttg aaaaatgaag gactggaagt tttagcagaa atcgcacgct tctgggcaga
    56281 ccgtgtccat ttttcaaaac gagcaaataa atacatgatc cacggtgtga ccggcccaaa
    56341 cgaatatgag aacaatatca ataacaattg gtatacaaat acgatcgcag cttgggtctt
    56401 gcgttacacg cgagaaaatt atttaactta tcaaaaagaa gctgctatca ctatcagcga
    56461 agaagaacta gccaaatgga aagatatcgc agagaacatg tacttcccag ttgacgaaga
    56521 attaggaatc tttgtccagc atgacacatt cttagataaa gatctcatgc ctgtatccga
    56581 tattcccaca agcgatcttc ctctgaatca gcattggtct tgggacaaaa tccttagatc
    56641 ttgcttcatc aagcaggcag atgtactaca ggggatttat ttcttcaatg atacttttac
    56701 aaaagaagaa aagcaaaaaa actttgattt ctatgaacca atgactgtcc atgagtcttc
    56761 cctttcgcca agtatccatt cgattttagc agcagaatta ggaatggaaa gcaaagctgt
    56821 agaaatgtac cagcgaacag ctcgcttgga tttggataat tacaataacg acacagaaga
    56881 cggcttgcac atcacttcca tgactggtag ctggttgtcg attgtccaag gatttgctca
    56941 aatgaaaact gcccatgatc agttgagatt cgcaccattt ctgccaaaaa cttggaaagc
    57001 ttattctttc catatcaatt atcgcggacg tttattgttc atctcagtca cagatgaaca
    57061 agttactttc gagttattag aaggttttcc tctagaaatt tacgtctatg aacagtctta
    57121 ttctttagcg gatcgtctag aaataacgct aagaaaggag gaagcaaatg tttaaagggg
    57181 tattgtttga tttagacggt gtcattaccg atactgctga attccattat cgtgcttggc
    57241 gaaagcttgg tcaggaaatc ggcattacga tcgaccacac attcaacgaa cagctaaaag
    57301 gcgtcagccg agaagactca ctctctttgc ttttagctta tggcggaaaa gaacactctt
    57361 tttcaaagga agaatttgca gaattagcga aaagaaaaaa cgattattat ttagagatga
    57421 tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt gcctttactg gattctttaa
    57481 aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa aaacgggcca ttcctacttg
    57541 aaaaaatggg tcttacacct tactttgatg ctattgccga cccggcagaa gcaacaaacg
    57601 gaaaacctgc gccagatatc ttcctccttg cagcaaaagc tgtcggacta aaagcagaag
    57661 aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc catcctctca agtggcgctc
    57721 aaccaattgg cgtgggacgt gctgaagacc ttggagagga gatcccgctt gtccctgata
    57781 cgacttttct tacgatcgat tacttgaaag aaaagtgtca tgaccatgga taatccagag
    57841 atctccgtcg taccagttcc agagactgaa ataaagaaaa tcacattgaa gaacgactac
    57901 ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc aaatcttcgc accagacaaa
    57961 gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt tcgaagatgt tctaacagac
    58021 aaaagctttt ttggagcaac tgtcggacct gtagccggaa ggatccggga tgcttcttgg
    58081 ggtgagcata gattggagaa aaactgcgga tcacaccata tccacggtgg cacgaaaggc
    58141 tggtctttcc aattttggga agtagaagtt ttcaaaactc catcatcaat aggtgtggtc
    58201 ttttacttaa aagatgaatt ttctacctat ccaggaccaa tcactgctac gatcacttac
    58261 cgtttaacaa aaaacgaatt agagatgaca acctctgcta gtagccttgt tgaaaccatc
    58321 tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa atggaacaag agacatctat
    58381 gaacatcagt taacggtctt tttagatggt gtgcttgaat tagatcaaga aaaactgcct
    58441 actggaaatt ggacaaaaaa agaaaaactg ccaatcgact ttagaaaatc cccaacactg
    58501 caagagatcc ttgcatgtta tccagatgga ctagatgatg tatttctttt gcatcatcct
    58561 aggctttcca aaacttcctt acaattattc gagaagcatt ctggtcgcca aatgactata
    58621 gcaacttcta ataaaagtat ggtattattt tctacgactg gattcgaagc tgatttctct
    58681 gtcaatggaa aacagatgca ttcaaactac ggattagcaa tcgaaccgca agaaatccct
    58741 gatatcgttc atttccccaa atttggatcg atcaacctcc atcctggtca agaacggatc
    58801 agtcagacga tttatcggtt ctctgcacaa taacaagaga aaggtgtttt tatcatggct
    58861 attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca cctcgaccgt ctctcgtgtc
    58921 atcaacgatc atcctagtat ttccgagaca acaaagaaga aagtccgaaa aattatggat
    58981 gaactaggtt atgttcctaa catgactgcc cgcaatctag gaaaacgcat ttccagtgcc
    59041 atcggcgtaa tcttaccccc tcttgattca aaagagcgga tcggtaaccc attttatctt
    59101 gagatcatgg aagcaatcaa cgaagaagcg cggaattatg atatgactac agcaatcgct
    59161 actgctaaaa attttgaatt gctgttagag aacgtccagc gtatgcattt acaaaaacaa
    59221 gtagatggtt ttattctagt gtattcagat agtcaagatc ctgtcattga ttatttatac
    59281 caaaaccaca tccctttcac tttgatcgga cagccttatc atcatgagaa cgaaatcgtt
    59341 tatgtagata atgataacca gcttctaggt aaacaagcaa cagaattttt gatccagaat
    59401 ggacataaga atatcctgtt tgtgacaaac acgactcatg aaagtttgta tttcgaacga
    59461 tatttcggtt atcaaaaagc aatgatgatg gctggtcttc ctgcgttatc cagtgtagta
    59521 ctagagcatc cggaggatta tctgtccttt gagaaaacgt tgaaagaaaa cgaagccaca
    59581 gcattggtgg tcattgacga tatcttcgcc cttcgcacga tgcagttggc gcaaatgtac
    59641 ggctatgtca ttccagagac actctccgtc attagcttca acaactcgat cttctcaaca
    59701 ttaacacacc cttaccttac aagtatcgat atcgatatct ccgaattagg acgtgtcgct
    59761 atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca gtggcgtacg tttggtcatc
    59821 cctcatcgct tgatcaaaag agagacagtc gttcctttag aaaacccata ctaaatagct
    59881 gactcactaa atacgctgac catttatcct atggtcagct ttttatgtaa aagaagatac
    59941 tacttctaac acattgttta tctgtaaaaa agagcgtggg acaaaagtaa agaacacttt
    60001 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cggaaataag
    60061 ctgaaattca caaaaattta aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt
    60121 aaacgcttct gtcccagcct cttttttcct tccttcaatt ttttacacgt cgacgattca
    60181 ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca
    60241 agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa tgtgccgctg actccccacg
    60301 gtacgagaga attgacggtc gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg
    60361 ataagccaac gtcatcaaaa gaacgcttga atgtttctcc tggaagaatg atggacagat
    60421 actgttctcc aacgatcaga ttgattccga tacagctcaa ggcagtaaac ccgactaaac
    60481 gagaaggtcg gtccatcttt tcttttaatt cttgaacgat ggtatcaacg atcagatatt
    60541 tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa actagcagaa cctaacatac
    60601 tcatgatccc gcctcgagaa agcagttcat ctagctttgg atcaggtgta tttgcgacat
    60661 aaccattcat caagatgtct gctgcttttc cgaccgtcaa acttttctca tggataaaag
    60721 aaagaacgag agcagctgtc gatcccgcta gtaatgtagg gatagccgga atttttttga
    60781 ttgccccccc aaataaaata actagtggaa tcaatgtcca aggagagatc caaaagctcg
    60841 ctttcatgtt ttggatcatt tcattgatac tagctagatc ggctgaatga ctgttatgtc
    60901 ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg gaaatccgta taacgcatac
    60961 cagcgatatg ttcgaataga ttaactcctc caatacccgc tgccaagttg gtcgtatctg
    61021 ataaaggcga gcagttattc cctaaaaagg caccggaaac gactgcgcct gtagtcattg
    61081 catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg
    61141 ttgtaaatga actgccgaca gtcactccga caattgcaca aacaagaaat gctgtcggta
    61201 aaaagaaacg cacagaaaca agtttaaatc cataaaccat aatcgtaggg atggttccag
    61261 ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc
    61321 ctggagtgat tcctttgatg atcccgtcat ggatctcatc ccatgtgaac tttttaaatc
    61381 tgccataaaa caacaaacac atgaaagctg caaggatcgg gatatgtggc gagagctgga
    61441 atccgatgat cagtactcct aggagtgcca acaacacgat caatacagcg atactttctt
    61501 ttaaagaaat atttttattc atcgtcgatc tcctatttca aatgaaaagg ccctgccatg
    61561 aaaaaactat tcacttggca ggacctccta ctaaatcagc gacaaaacgt cggctgaaag
    61621 acttattttt tttatttttc ttctaaactt ttttctacct caatccccca agaatctttc
    61681 ttgaagtgat tgatgatcaa tggaataacg aacatgacca ctgtgatagc tgctactaat
    61741 aatgcatcac tcaatgtcgc tgtacctaat gtagcaaata cgacaacgat gatagagaag
    61801 atcagcatgc agctgactac ccaagctaat ccgtttccgt ttttaccaat tcggtaagga
    61861 cgttcgaatt ctggatgttt cttacgcatt ttaagcaatg aaacagcaat caaaatgtac
    61921 acgatacaat aaataacagt tgtagcattt gtaagtgtca agaatacagc acttacgtca
    61981 tccattacac cataaagcaa agcaaataat gaaataacga ttgattgtgt tagaacgaca
    62041 tttctagata cgccgtattt gttttcttta tggaatccga attttggtgg aaggaatcct
    62101 tcacgagcta cttggatgat tgtttttgat ggtccagtaa cccaagctga taattgtaaa
    62161 agcacaccaa taaataccat gaagctgaag atatttccga tcacttctgg taagcctagg
    62221 atttgacagt caatcaagat aggctgcgtg atatttgcta attccatttt tccatctggt
    62281 acgacatttg ctacaagcat cgcattgatg acattcaata aaactaatcc gatcaaagcg
    62341 ataaatacgc cttttgtata attttttgtt gcgtctttca aacgaggcat gtaaacagaa
    62401 ctcatttcga tcccaacaaa aataaatgtg ataccagcta agtatttcaa tgaatcaata
    62461 tgttccaagt cagggaaagc ttttgaccat gaaaagtcac ctagatagcc gtttgagact
    62521 agtccgactt tgaaggctgc caaaacacct aaaacgaaca tgatgactac tgggatatat
    62581 acccctagcc atacaccgat gttcccgccg acttttgcca tatcgaattt caagttcaag
    62641 atcgtgatga tccagtagat aactaaaata caacctaaaa cgaaccagtg attattgcca
    62701 agttctacat taccaaatgt gtttcctaac aatggtccta aagtagaagc gaccattacc
    62761 atacctggga acatttgcac ccataatagc catgcaacga caaatcccca tttgctgccc
    62821 agtgctgttt ttacccataa ctgaggaccg ccttcttgag gaagcatcgt ggaaagctca
    62881 ccggaaacca ttgatatcgg accggcaaaa aacaaaactg caaaaattga ataaaaaatc
    62941 aatgtccaac cgactgcggc taacgtagga atactccgaa ccgttgcaca cagtgccatt
    63001 gtcagaccga taaaggtccc aagacccatt ttacttactt cagatttact caccgttgac
    63061 actctccttg attttttgga taaaataacg gtgtctttga agtagtttca gcaataaacg
    63121 aaacaattca gcgaatattt gaggggattc tttgagtcta ttgcttgaag ggtttcttca
    63181 aagacaacct caaaacaaag ggatcgaaga gcttaggtct ccccttgctc ttcagatcca
    63241 aacatgcgtc agaaacagtt agtattcaag ctgttttcta acatgattac cttgcttatt
    63301 ttgcttcgct tgccaataat tggtctgcgt agattttttc caatttttct tgaagagctg
    63361 ctttgatttt ttcagcatat tcttcaaagt gttcttcttt gttcatgtat ggtgtcatta
    63421 cgctggcacg caatacagtt acttttccgg cacggttcca ttcttcatct gagaatccta
    63481 gttggttcac aaattgtaat gggctgttgc catagtctgg gatagcaaag tcagtatgtg
    63541 aagttaagaa ctcattaccg taaatgcttc ctttcacgta tgaagaataa tcatatacgt
    63601 catggttcaa tttattcatt gcaactaagt catcgttgcc tttttctttg aatacatagt
    63661 ctaccatatt gaaatctgga taagttaatg gatgaacttc gatttctttg tctcctactt
    63721 taaatgacaa gtcattcaag aagttgtaga aacgatgtgc accttcgatt gatgcaccca
    63781 ttaatttacc atatcctgtg acattcaaag gtaagacatg gtgtgctgcc caaacgctgg
    63841 ctgctgttgc ccctgctttt gaaccttcca agatataagc cccaagtaat gctgggatat
    63901 ctgcgccttt ttcaaataca tatgtcgcaa agtaagagat aacgtctctc atgcggatat
    63961 ctttgatgac gataccaccg gcagaatatg gaacatagcc cattttatgt gggtcaatcg
    64021 ttacagattc tgcttcttcg attgctttat atgcactgtg tacttcttct aagatataat
    64081 ctttgttttc tgtaaagaca ttgtatttgt aatgaacatc ttttaaatct tcaaatggaa
    64141 tgaagttgtt gtcttcgtct aagaaaatcg cacggccgta tccgccataa gcagcatcta
    64201 cgtgtaagta gaagtaaata ccgtcttttt ctaatacgcg acgcaatgca acgattttat
    64261 cgataccgtc gattgctcct tcttctgttg atcctacaac accgactaca cctaggattg
    64321 gtgttttttc agctgctaga ccacgaacga ttttttcaag ttcgttgatg tccatgcgat
    64381 agttgtggtc aactggcact ggaatcactt ggtctaaacc gataccgatg atgtcggcag
    64441 cttttagcca tgaatagtgt ttagtttgtg gaaccaacca ttttcctaat ttttctaaat
    64501 gttttccgct acgtgctgag tgagctttga tttcgtcgat tttttctggg acgctgtcta
    64561 acagattcat gatttcttct gttgacaagt tcattaattc ccaatcgctt tttccagcaa
    64621 ctagttcagg tgtcacttct ttcattgcaa gcggcaatga tttgatgttg cgggcatacc
    64681 aaagtccttc taagtttgct aaagaaccat cagcaacgat gtggccccag ccatctttat
    64741 agctcattaa ttttgcaaat tccattccta cttcttcttc catttggctt gttgcaggag
    64801 aagattcata ggcaacgtta ttaccattcc atagcatagc aaaattgtac gctagtaaag
    64861 atggcatcaa tgtttcagag ttcatatgac cccagtaacg tccggcatta tgccaaggaa
    64921 cggaatgggt acgcatccgc gctgaaattt ctgatagaac atcctttgtt ttattcacag
    64981 tgtgttcaaa actagcactg cttttttctt ctggcgtaat aataggcatg tcttgaggca
    65041 tgtagttttg acgccagcct aaatgttcat ccaccaattc attcaacaaa gctttataga
    65101 tttggccgtt ctcagctttg tccccaatga atagggcatt taggtttaga tctttcgaca
    65161 atgattcact catagtgaaa ccctcctaaa aaaatattta aattttgtcg agccctacat
    65221 tacacctgtt cttctattct gtaaactaag ttttggcctg atttttaatc atattttaat
    65281 atgtatcaat tatccgaatt gttcgcactt ttgaaataat taaaaaaaac gagggaattt
    65341 acaaatgtaa actccctcgc aaagattgta ttaacgacaa ccagcgttct attttaggat
    65401 gggtttcaaa attggaatgt tgtaatagta tttccctttt tcaaggtaaa aacttactaa
    65461 attctaatca attactttgg ccaaaaagta atttttctta ccttttcgaa taaccacaaa
    65521 cttaccatcg aattcatcgg atggatttat gacaaaattt aaatcggtaa cgcggtcacc
    65581 gttaatactt atagctccgt ttgaaacatc ttcacgtgct tgacgtttag atggttcaat
    65641 ttttgtggaa acaagcagtt ccacgatatt ttcaggtgtg cttgaaattt caacgttcgg
    65701 cattttaccg aatccttgcg cgatttcttc cgcatttagt tctttaatat ttccagagaa
    65761 caatgcttgc gttatttttt gcgcttcttt caagtcttct tcactatgca caaatcttgt
    65821 aacttcttct gccaaacggc gttgtgcttc acgtttctca ggttctgtct ctactttttt
    65881 cgctaagtct tcgatttctt cttgagacaa gaaagtaaag aatttcaagt atttcactac
    65941 atcgcgatca tcttgattca accagaattg gtagaactca aatggtgaag ttttcttcgg
    66001 atcaagccaa acggcaccgc ctgcagtttt tccgaatttc gttccgtcag ccttcagcat
    66061 caatgggatc gttaaaccga acgcttctac ttctggtcct tctaattttc ggattaggtc
    66121 taatccagct gtgatatttc cccattggtc tgctccaccg atttgcaatt gcacatcgta
    66181 tgttttatgc agatgcaaaa agtcgataga ttgcagaatt tggtaagtaa actctgtaaa
    66241 tgagattcct acttccaaac ggctggcaac gatatctttt gctaacatcg tgttgatgtt
    66301 gaagttttta ccgtagtctc tcaaaaattc aagtaaagag atttttgaca accagtcgta
    66361 gttgttcaca aatgtgatat ttgcatcttt accaaacaat tttctcattt gattggaaag
    66421 tgcgtccacg ttatgttgca cttgttccat cgtttgcaac acacgttctg ttttgcggcc
    66481 gcttgggtcg ccgatcgttc ctgttccgcc gccaatcaga atatatggat gatggcctgc
    66541 caattggaat cttttcatca tcataaatgg aatcaaatgt ccgatatgca tcgaatcacc
    66601 agttggatca acgccgcaat aaagtgaaat gctcttttct tcaacgagct cgcgaagtct
    66661 ttcttcgttt gtttgctgat taatggcgtc gcgccaagtt aattcatcaa taatattcat
    66721 tttactgacc tccgtgtagt ttttttatga aagcaggaac ctgctagcat gtgtaattaa
    66781 ttatttcttc aggataggat aggaaataaa aaaagtccct tggcatataa gccaagggac
    66841 gaaatctcgc ggtaccaccc aatttgtatg tctagatatc caacatacct ctcgaacatt
    66901 taacgctgtc gcgtggcaat cactgccaaa gactcctgaa atgtaattcg ctttaaagca
    66961 tgtactggtt tgcagcgacc accagctttc tgaaacattg gctttaacaa ctacttcgtt
    67021 tcgatcttcg tcttgttatt attaatatgt gtctatcata gagcgcaact ttttctttgt
    67081 caacagaaaa aggatatttt ttttaaattc ctcatttttt cttttttgaa tccgctctaa
    67141 ttccgaacat tttattttca attattgtta aaagaaataa ctttttctgt attttttctg
    67201 caatttatcg agaagtcact ttactttgcc tcttattctt tcttcgttac aataaggaat
    67261 gggacaagta aaaaaatgaa aaagcaaggg acgaggtata tggaaaaaaa gcaaaaaata
    67321 attttaggag ccacttctgc tttaactatc gttattttaa ttctaagttt gttttttgtc
    67381 atccgatcat ctacaaaaaa agaaccaatc attcctgaaa aaaaaataac agaaaaagca
    67441 aaagaaccga cagatgataa gcaacaacta gttactcaag caaaaaatct ggctcaaagc
    67501 tacgattatg accaagcgat tgcgttattg gaaaaagata gtaagaacgg atctactgaa
    67561 acacaacagc ttcttcaaac actaaaaaag gagaaagaac aactgatcga ctggtctgat
    67621 cctactcaga tctctcatgt ctttttccac agcttgatcg tggacccgca aaaagccttc
    67681 cactctcaac aggcacaagg atacaaagat tatatggtga ctgttgaaga attcaaccgt
    67741 tcgatcgaac aactttatca aaatgatttt gttttagtta atttaaatga tttgatccaa
    67801 aaagacgaac aaggaaatct ttcctttaac gggcttgcgc tacctgaagg aaaaaaacca
    67861 ttggttcttt cacaagatga tgtcagctat tatgaatata tggataattc cggttttcct
    67921 agccgattgg tcgtatcaaa taacaatgaa atcaaaaatg tctataaaga gaaagataaa
    67981 gaagaaatcg gtgactttga catggttcct ctgatcgatg cctttgtcaa gaaacatcct
    68041 gatttttctt atcgaggcgc aaaaggtacc ttggccctga caggttacaa cggagtttta
    68101 ggttatcgaa catcaaaatc tcaatatgga gacaatgaaa aaacaaaaaa agagattaaa
    68161 gaagctaaaa aagtggcgga acagttgaag aaagacggct ggagctttgc ttcccataca
    68221 tggggtcact tgaatatgac tcaagcttca ctagaagatg taaaaaaaga caatgaattg
    68281 tggcagcaag aagttgcacc tatcctagga aaaacaaata ttctgattta tccatttggt
    68341 gcagacatca gcgactggca gccttattca gcagaaaatc aaaaatttgc ttacttgaag
    68401 agtcaaggtt tcgatatttt ctgtaatgtc gatgcttcta caccagcttg gggacaatta
    68461 ggtacggatt tctatcgaaa tgctcggatc aatatcgatg gcattcgttt tcaatctgat
    68521 ttagacggaa aaaatcctat tcttgatcca ttcatcaatg tccatgatgt ctatgacaaa
    68581 gaagcacgcg aatctgcttg attttacgtc atttatgaac caaaaaagcc gaactgacat
    68641 tttatcgtca gtttcggctt ttttatgctt tatttcttct tcggtgcgat tcccttgatc
    68701 aggacttctg tccattctgc tgtatatgga agcagttttg cgtatatctc cggcttagaa
    68761 attccttcta ccattgcttg aaaatagagg atcaagaact catccgaata tttcaaatcc
    68821 acttttcctt ctttccgccc tcgatggaaa agatcaagca tgatactgaa actttcttgc
    68881 gtatacgcat ccatcgcgtc agaaaattct tttttttctt tttttgtata gtatgtcatc
    68941 aaatcgatat aaaattgttt gttcactcga ttgagataac ggattttgtt ttcactcatg
    69001 gcaatcagtg tttcttcaaa tggaagattt tccgcaatca acgctttcgc ttcttgccct
    69061 aaatcattca taaatgcaaa aaatacttca taaatcagcc cttccttgct gccaaaatat
    69121 ttaaaaatcg tcgctttaat tacctgagcg ttctcagcta ccttatccat tgtcactcca
    69181 gcggttccta tgtctgtgtt catcaagtca aaagctgctt gtaaaatctc tttctttttc
    69241 tcttgtgctc tcttttcaaa accgttcatt tgtagagcct cccgtaattt taaactttta
    69301 taatatatat agttcaaatt tattctgagg tttattataa ctatctattg agaaagtatc
    69361 aagctagcaa ttccttgttt atgaattcat tgacagaaat ttataaaaag aatattatgt
    69421 actaaaataa taaaaaaagt tttataaaaa ggtataaaac ctttttatta aactatttaa
    69481 tcttgtttag tatatatttt tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa
    69541 gtcacacatt tgaaaaagag ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc
    69601 gtacattcag gtgaagtcgt tggatttatc ggaccaaatg gttctggaaa atccacgacg
    69661 attcggacac ttttagggat tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa
    69721 gatgtatggg aagatagttt atctatccat gaacgtcttt cttatgttcc aggcgatatc
    69781 tctttgtggg gaaatttgac tggaggagcg atcatcgatc tcttcatgaa gcttcatgga
    69841 aaagggagtg ctcaaaaaaa agatgaactg attcaacgtt tctctcttga tcctaagaaa
    69901 aaagcaaaaa actattcaaa aggaaatcgc caaaaagtgg gcttgattgc tgctttggct
    69961 gttgattcgg atctctattt atttgatgag cctacttctg gtttagatcc tttgatggaa
    70021 acgattttcc aagaagaagt tgaaaaattg aaagaacagg gcaaatccat cctactttct
    70081 tctcatattc tcagtgaagt cgagcgacta gcggataaag tcgtgatcat acgcgaagga
    70141 acagtcgtag aagaaggaac actcgatgaa ttgcgtcatt tgactcgttc gaccatcact
    70201 atagaaacag aagcagagaa tagtcactta gcacaattga acggtgtcca tgactttgtg
    70261 caaaaaggtc acctcgctca atttgctgct gataacgaag ctttcgatgt gattttagct
    70321 gaagcgtcca aattaggtgt gaaaaaattt gaatctattc caccgactct tgaggattta
    70381 ttcatgagac attatgaagg atgaggagga agcgaaatga ctgataaatt ttcacgctgg
    70441 tccacccttt tttctcaaaa ccttagccgt gactggaaaa agatcctgat atggctagtc
    70501 ggtgtcactg ctttttctgg tggttttgtc cctgcgtttg aagaattgac ttccggacaa
    70561 ggattagctg gattatacga aaccttgcaa aacccggcaa tgattgcaat ggtcggacca
    70621 actccagctt cgaatgctgc tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg
    70681 cttttttgca gtttgtttgc tgctgtgatc tctgttttac acgtggtgag ccaaacgaga
    70741 aaaaaagaag aacttggtct gattgaactt attcgttcct ttcgagtcgg acgtcaagca
    70801 aactctttag ctgtcatcgc tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc
    70861 agctgtacga tgattttttt ccaagcagat tcgatcactg cagaaggtgc ttggcttttt
    70921 gggctatctg taggtagtat ggggattttg gggagtgccg ttgcactgtt tgctgcacaa
    70981 ctgatgccaa gttcagccag ttcgaatggt ctgtctttag cgtttttagg tggactttac
    71041 cttctacgag caataacaga tgtagctgat tcctcccttt cgatgtggaa tcctttaggc
    71101 tggtcttatt tgacctatcc atttacaact aacaactgga ttcctttgct ttatttgttt
    71161 ttgttcagtc taatcttgat aatcagttca tttgttttag aggaacatcg agatttgaac
    71221 accggctata tccccgaacg tgaaggaaaa ccgtatgcta aacgttctct tttatccttg
    71281 cgtggtttat tattctatct aaataaaggg atgacaatca gttggcttat cacttttggt
    71341 ttgatggggg cggcatacgg ctctatctac gggaatatgc aacagttttt agaaagtaac
    71401 gacttaatga aacagatgtt tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc
    71461 acgatcatgg tcgtcttgat ttgtctagct gctattttac caattgctag tacagaagaa
    71521 acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg
    71581 accaatatgt tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca
    71641 ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt
    71701 cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt
    71761 ctattcggat ggcttcctcg aatgggtaaa cttgcttata tctacttagc ttactccttt
    71821 gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc
    71881 cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta
    71941 tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta
    72001 ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa
    72061 atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga
    72121 tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc
    72181 tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca
    72241 ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt
    72301 cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa
    72361 cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat
    72421 ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat
    72481 ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa
    72541 gctgttcctt tccgctagca gataattgat acactttcat actgccggag gccacgatca
    72601 ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt
    72661 tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa
    72721 atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa
    72781 taaagaaaca taacttccct tattccaact gataattttc ttttatcata gcataaacgt
    72841 caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc
    72901 ttcttttgag accttaagta attgattcct atcaattttt taaatattta ctttttgaat
    72961 aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat
    73021 ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt
    73081 gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt
    73141 tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat
    73201 cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata
    73261 tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt
    73321 tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga
    73381 tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt
    73441 cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga
    73501 ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct
    73561 ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg
    73621 gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg
    73681 attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt
    73741 gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc
    73801 aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac
    73861 atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat
    73921 tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc
    73981 ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg
    74041 cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc
    74101 cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc
    74161 ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc
    74221 gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg
    74281 atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc
    74341 tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc
    74401 tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt
    74461 ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca
    74521 ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat
    74581 aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac
    74641 atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac
    74701 acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc
    74761 cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac
    74821 accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt
    74881 tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc
    74941 tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag
    75001 gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc
    75061 atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat
    75121 atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact
    75181 atccgcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc
    75241 aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc
    75301 gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg
    75361 ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc
    75421 ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc
    75481 cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg
    75541 ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct
    75601 cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa
    75661 aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt
    75721 ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt
    75781 gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca
    75841 aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg
    75901 ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa gatatgtgta
    75961 gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac
    76021 aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga
    76081 ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg
    76141 cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga
    76201 aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta
    76261 tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc
    76321 cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc
    76381 actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt
    76441 tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta
    76501 aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt
    76561 tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt
    76621 ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt
    76681 ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac
    76741 tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct
    76801 gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga
    76861 cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc
    76921 ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt
    76981 tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca
    77041 aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa
    77101 tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt
    77161 tttcagcacc agaagagctt atagttgttg aaggttctgt ttgagcaggt tcagaagttt
    77221 ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg
    77281 gcttcattct ttttttcatc acttttccca tccttactac tttttaacta atttcttatt
    77341 ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg
    77401 catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat
    77461 cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa
    77521 aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat
    77581 cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact
    77641 gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa
    77701 tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg
    77761 atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa
    77821 attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctttgtc gatatagcat
    77881 cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca
    77941 gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa
    78001 tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata
    78061 aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc
    78121 aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac
    78181 cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc
    78241 tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag
    78301 tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt
    78361 agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc
    78421 tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtatc
    78481 ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact
    78541 caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac
    78601 accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa atacataata
    78661 gacaaacaag tatctaagaa taaattacct gtatgggtga ttgagatcat ttcagttcca
    78721 ccccattttc tttatacaaa tctgcaatga atgtcgtatc tagatttttc tcttcggaga
    78781 cagaataaaa acggaccgtt tctctctctt cttttgttcc aaaacgttcc gtatacactt
    78841 cttcaagtaa ttcttgtctt tttttcaatt tttcatcttc atattcaaaa gatttattta
    78901 cttttttgta tagatttcga ttgttccacc tagtcaatac aaatgaggta atcaggaaac
    78961 tgaaaaaaat caatacaaaa aagccgatta agaaatacag cattttcttt tctgataaaa
    79021 aagcgtacaa ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa aagacccata
    79081 taaaaggaag aacaacgatc atccatccta aaagagccac taacgtttgc caaatcttta
    79141 accagtaatt accttttgaa aaatacttat ctttcaccaa aattgatgca acttcttctc
    79201 ttgttacttt tttcatatcc tcacctcacg tttttttgtt acgacgtagg agtttaccat
    79261 acatttcatt tcttttcaca taaagtgttc ctaaattttt cctaagaaaa agaacaaaac
    79321 atttctcatc agcaaatgaa aaatattttg ttctttttat cagttgttct ttttattttt
    79381 tatctcttaa ttatgttgct ctttccaata cgacaatgct tttcattcct ctagatatcg
    79441 ccgtgtacat ttgtctgcgt cttctttgct catctccatc gttttcaaat ggatataaga
    79501 ttacatgatc aaattccagt ccttttgcta gactcaaagg aatgatttgg cagttttcta
    79561 atccatatga atttttttta atcgattctt gtagcgtttg ggtttcctct tctgtttggg
    79621 tgataactgc cgctttttct ccttttagag aaaccaaaat ctcagctagt attttcaaca
    79681 attcttcaga ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt cggatcgata
    79741 cgatgtcgat cttctcttga tccacagcat aggttttgaa aagctctgtg attgctccgc
    79801 tagaacggta actatttcta agatcatacc gatggatcgg cagatggtag tcgtcgaaac
    79861 aacgcataat atcagcaaaa gtagtggaag aattaaagat tgcctgattc tcgtcaccta
    79921 ctaaagtaaa gtgagttttt ggaaataatt cgatcaatag acctaattgt gccagggtat
    79981 aatcttgtac ttcatcaatc aatagatagc gaaatgctgg tacatttacc ttttcaacaa
    80041 gtaaatgacg aatagctaat ataatgactg cttcatcaac agtacgtgtg ggttgccaag
    80101 cataagctga gccatatcgt ttttcataaa ttctttcaaa caactgatgc tcatccaccc
    80161 atgccatttc ttcgatttgt ctggttattt ttcgatattt tttttgtaaa agtttccttg
    80221 cataagctac aatactttgt tccgaatcat cagaaatcaa tttaccgaat aattccaact
    80281 gcctatcttc tgtcaacgag agtacctgat tctgcacagc aggacttttt gcttgtttca
    80341 aaagatgatt cttccattcg ctcatcagtg cctttttcgt tgcttgaagc ctatcgatca
    80401 atttaggatg agcaggaact ttctcatata ttttttcgat cgttttttta gaaaggactg
    80461 tttttccatt tttagtaagt ccacggataa aattcggatg atctaaaaac aaaggatctg
    80521 attgttttag attatcgata aactttttgc tacgcaaacg ttctgtctgc tccgatacat
    80581 tttctcccgt gatacgtttg aaataagcct cttcaccttc tatttcctct gctgaaagct
    80641 gtgaaaccag ctgtatgatg gttagattca gtggatttct ttcacctaat gatggtagga
    80701 cattcgaaat atagtcgata aaccgatgat taggtgatag aatcagcaag tcatcggatg
    80761 tcatcgtatc tcgatataaa tagagcagat acgcaatacg ctgcatgata gcagaggttt
    80821 taccacttcc tgctacgcca tttaccaaga tattttgact tttcacatcc cgaatgattg
    80881 tgttttgttc cgattggatc gtagcagtga tagccttcat ttcattggtc tcatcttggc
    80941 ctaagacttc taataatacg tcatcttgaa tagctacaga cgtatcaaaa aacttgacca
    81001 aacgatctct ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg attttatttt
    81061 gacgaaccgt ataagaagat ggaccaatca tgttattgta aaaaagttcg gcaattggtg
    81121 aacgccaatc ataaacaaga ttttcatctg catcatctgt gaaatttgct gtaccgatat
    81181 aaaaggattc ttttggctca ttatccagaa agtctacaac gacttttcca aagtagggac
    81241 ttttcaataa acgttcgatc tttgctagct gattttctgc gtttttctgt ttgatattca
    81301 tctgatcgat ttctctattt ttcatttcaa tcatcgaaaa agtatccagg ttatctaagt
    81361 aactgtcaaa attcaatcga agctcgccag ccatttcttt caacgactgg tttccttcta
    81421 tttttgccgt atccatcgat tcccgcagtt cgtttttttt atcaattagc ttttgataca
    81481 cgttcttcag atatgcttct tctaactgtt tttgtgtgtt gaccatacac attctcctct
    81541 ttttttaaag tggtggaacc gattagttta cctcttttaa gagtcgaatg caagcttttt
    81601 aacgagtatc atttgttagg atatatcagt tcttcaggtc ggacagtttg atataaagaa
    81661 aatgcaccat caaggttttt tacgtggtag ccagactgct tcaagattct ttccgctaga
    81721 tagctacgta atccgctatg acagctgacg atatattctt gttggctgtc taattcattt
    81781 aatcggtccc gaagttcatt caaagggata tgtttggctt ccttgaattt gcccttttcc
    81841 aattcttctg ctgttcgcac atctaataaa atttttcctt ttgctagttc tgtcggcaat
    81901 tcatgccatt gtacggtttc actaagccct tctgcaatgt tcattgctgc atatcctagc
    81961 atgtttactg gatctttggc tgagccaaat ggcggtgcat acgtcaattc taattccggt
    82021 aaatcgaaga tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat ccgtttatcc
    82081 acaccttttg ccccaacacc ttgtgcacca taaatctcgc cagtctctgg atggaagatc
    82141 aacttcagta aaatatctgt tgcttctgga taatagcttg catgatcttt tcctgataca
    82201 tgaatgactt caaaagacag ttcgttttct ttggctgttc tttcacttaa accggtggat
    82261 gcggcagtca ttccaaacgt tcgtataatt gctgttccaa taccgccttg atttcttcga
    82321 gaaattccgg aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg tgaagcaaga
    82381 gaaataagtg catcttttcc agtgatttgc tgttttacaa cgatagcatc ccccaccgca
    82441 taaatatcag gaatattcgt ttgataacgt tcgtctacta ggataccccc gcgtatgcct
    82501 aattcgattc cagcggtcac tgctagctgg ttatcaggac gaacaccaat ggataaaata
    82561 actaaatcag cagggatggt acgcccatca tccagtcgca atcgtttccc gtggtcttca
    82621 aatccagcga cagcatgaga agtaatcact tctactcctt gatgagacaa ttctttttct
    82681 ataaaagctg ccatttcttc atccagtgtt ggaagaatat gtggtgcttg ttcgatcacc
    82741 atgacttcta aacctcttct ttttaggttt tctgccattt ccaatccgat gaatcctgca
    82801 ccaacgatca cggcacgctt tggctgtttt tctaatgcat gcatcacttt atcgatatct
    82861 ggtacatttc taatagaaaa aacattgtcg gcttctgtta tccctggaat cgatggaaca
    82921 actggttttg caccaggaga taaaaccaat gcatcgtact gttcaatgtg tttttgaccc
    82981 tcatgtacga cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc atgatttgga
    83041 cgcacatcta aacaaaaacg agcggataat gattctggcg tttggacaag aagattttcc
    83101 cgttcactga tttctcccga aagataataa ggcaagccgc aatttgcaaa agaaacatac
    83161 ggtccttttt caaaaacaac gatttctgca tcttccatta atcggcgaag ccgtgtagca
    83221 gcagacattc cacctgctac acctccgaca ataacgattt tcatcttatt tccctcctac
    83281 tgttcgatca aaccattgat tcatgccacc acgtacattc actacatcat aaccagattt
    83341 ctttaattct ttagctgctt gtttactcct catccctgat tgacaaatca catagacggt
    83401 cttttctttg tctccatcat atctgtttat actttgtaat ggaacatttt tagctccttt
    83461 tatatgcccg ccccggtatt ctgcaggtgt tctaacatca agtaacgctg gattttccaa
    83521 aagcttttcc tttaattctg ggactgttat cgattttatt gattgaaata gcaaattcat
    83581 tttctgcacc tcatttacat ttttataaat acctcatggg gtatattaaa caggagcttt
    83641 attaatatgt caaccagaat gactcttcat ttttttcact actttttata tttgcataaa
    83701 ataccgataa ggggtattat ctaattataa aaatagagga gaatgtgtta tggagattga
    83761 taataaaaat atcctgaacc gtttgaaacg tacagaggga caaattcgtg gtatccaaaa
    83821 aatgatcgaa gacgaaaaag aatgtatgga tgtcatcact caattaagcg ctgttcgttc
    83881 tagtattgat cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt gctttgagaa
    83941 acctgatagt aatccagaag aacaagcggc taaactagaa caagcgatca agatgattat
    84001 taaaaagtaa aggaatgatt tcaatgaaag tcgttttcca tattgatgaa ttagaaaaat
    84061 ggtcagaaac agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa acgacgattg
    84121 tcgtatctgt aaatggcatc gcgattaccg gttatctaga ctctgccaat gcagaattct
    84181 tagatttgca aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct aatcatattt
    84241 cagaaagttc tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt gatttagttg
    84301 aactgcaatc ccaaggttat gcttatatca agccttagtc agtagaggtt gcgacagaag
    84361 tgatcagctt caagaaattt cggcttattt ttcgaagaag ctacttctgg aaaaccgtga
    84421 atcaaaggtt gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
    84481 cttctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    84541 ttaaggaaga ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa aaacgggaaa
    84601 atagctcctc atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc
    84661 gtttaaggaa gagattgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
    84721 aaatggattt ctacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaat
    84781 accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga gaaacaaacg
    84841 gaaaaatagc tgctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga
    84901 acaccgttta ccaagaggtt gtgagcctac catttaactc cgaatcacaa gaaataattt
    84961 caaaaacaac ttctcctgtt tttggtttgg ttggcttatt atcgagaagt agctactggg
    85021 agtctattta tcaaaaaaag gaatgcgaca taggtcacac ccctttttta ttttctttga
    85081 aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc gtaagtgatg
    85141 acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag caaaaggatc
    85201 atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc acttaatttg
    85261 atatttttag atagcccgat taatccagct acaaacataa agccttcttt tatcaagaat
    85321 agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat cagaaaaagt
    85381 tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac ctgattatat
    85441 ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat gaccgctcct
    85501 gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa aaaaatacgg
    85561 aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt gcttaatttc
    85621 attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac acggggaata
    85681 aaaccaatga tttcactgtt tttatttttc tttacagcca ctgatattat gagctaattt
    85741 gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt ttgcgcattt
    85801 cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc tgccgtttac
    85861 gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac agatcttctg
    85921 ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg tctaaatata
    85981 catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg tattcattga
    86041 caaatatccg atcacctttt gcaaagagct ggcgttcttt tttattctcc ttcctatttg
    86101 taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt tccgccaagt
    86161 aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca atccacaaag
    86221 cttggctttc tcctgtttct ccaacccgca actgatattt aggacgaagt gcttcccgat
    86281 cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc ttgatttctc
    86341 cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc atgattgcga
    86401 tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct aaaagtacca
    86461 acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga ccagaaaatg
    86521 tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc tcatcaaaga
    86581 cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc atcaaagtca
    86641 ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg gtgataatca
    86701 agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca ggtaaaaatg
    86761 gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt gtgcttccat
    86821 tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg atcgtttcga
    86881 tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc gccaggactt
    86941 gatattcttc actgatttct tcagctttta acaactgata gtgttcattc aatttagaaa
    87001 tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata atcgttccat
    87061 ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct ttttgaacaa
    87121 tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc agtaattttg
    87181 actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa ttacgtgacg
    87241 cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct acatttatca
    87301 aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga ttcttatcaa
    87361 aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc aaatcggttg
    87421 gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc ggcaacccca
    87481 taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt tcttcttgac
    87541 gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt tttttgctat
    87601 agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt aattgactaa
    87661 tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca aaataaataa
    87721 gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc ctatccttat
    87781 tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac caaataaact
    87841 ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa aacatacccc
    87901 attcttctag gtcttattta ttttccactt aactatagca agacacattt tggttgtcaa
    87961 actttagtat gttttataga tttacaccat taatgaatag acacctaccg cgataattgc
    88021 tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag ttcctttatt
    88081 agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag ggttgattgc
    88141 atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac ctacagcgaa
    88201 tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa gaactaatac
    88261 aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag caggacctgt
    88321 tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg gcaaataaga
    88381 aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaatga tgaaaggaac
    88441 gacaagattc caactaattg cccctgtcat cgccatagct agagagactg caggattcaa
    88501 atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga gtccccaacc
    88561 aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat tcacagccgc
    88621 acacacacca tctccaagca aaactaggat cagtgtccca ataaactctc ccatgatttg
    88681 agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg attctgcaat
    88741 caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt caaaatatcc
    88801 agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt caaacaacag
    88861 atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt cttctaaacc
    88921 ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca tttcttttgc
    88981 taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag tagcatcttc
    89041 ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt cttctaattt
    89101 agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt ctttcgtctc
    89161 tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt ctgccatctt
    89221 acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt ctctttctaa
    89281 agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt ccaaatggtt
    89341 caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac ttactgtatc
    89401 gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat tgtaatcaga
    89461 accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga tatcattcaa
    89521 tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat ctacatcttc
    89581 ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg taccaaagta
    89641 agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc ctgtatcgaa
    89701 gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga tccctttagt
    89761 tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt tatctaccca
    89821 aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat ctgtcagcag
    89881 gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa ccgctttcat
    89941 tttacttact agataagccc catcttcggc agcctttttg atattatcaa tcactaaacg
    90001 ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt cttttttcaa
    90061 aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat atttggttcc
    90121 tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga acatattgaa
    90181 ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt ttgggatgtg
    90241 tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta cttccacatc
    90301 aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag aggaagtccc
    90361 ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg ctgactggac
    90421 tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt cttcttgctg
    90481 cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt cctccttatt
    90541 caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt tctcggcgct
    90601 cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg atctcgtctg
    90661 tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc gccgttgttt
    90721 ccaagttttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat tgcatcagat
    90781 attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt ccagtgtcat
    90841 cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa gtagctttaa
    90901 tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt gctgcctgat
    90961 cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca tcttcgcttg
    91021 tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga agtccatctc
    91081 tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca tagtaaacct
    91141 ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat tgcggttttt
    91201 caccggtatt catgacaata aatgaacctg taccataagt attcttgacc atgcctggtt
    91261 cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct gaaattggtg
    91321 cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag ttagaagtga
    91381 cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga tcccaatcta
    91441 gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta acatgtgctt
    91501 gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc aattcgcctt
    91561 tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc ttcgttgctg
    91621 agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga tagccttcat
    91681 tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact gcatgataga
    91741 ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc gttatgccaa
    91801 tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca ataactgact
    91861 gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc gggaaatatt
    91921 gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag ataattgctc
    91981 gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc attgtgattc
    92041 cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt tttaaaaaaa
    92101 gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt atgaagctat
    92161 tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat cgaataatgt
    92221 cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt ctctggcaaa
    92281 atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca ataccgccga
    92341 ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc ggcagacttt
    92401 ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc aaaagaacat
    92461 tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc ctcggcaatt
    92521 gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc ccgagcattt
    92581 tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca acaaagaaaa
    92641 accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct ttgattttca
    92701 aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct aaaacagcgg
    92761 ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg tctactatcg
    92821 aaaaagaaac aaaaaagctg taaaacatca ttgtttcgta tacattcagt tctgctgctg
    92881 ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg tccgactgat
    92941 agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat agtctttttt
    93001 tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac gttgtttcta
    93061 atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc aaaaaaagcg
    93121 gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt atctgtagct
    93181 cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag agctggttca
    93241 attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc gctaatcgaa
    93301 cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc aagctttcct
    93361 gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca agcagcgcct
    93421 gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc aattcaatat
    93481 cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc aaaccattga
    93541 tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt aactgctctt
    93601 ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga ccacaccata
    93661 tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg ttcaaacgtc
    93721 catccttctt ctactgcaat tgtcagttgg tcaagaaggt ggaacatctc ttttttgctt
    93781 tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag tttaactttg
    93841 ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt ttggggataa
    93901 aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa ccactgattg
    93961 ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt cttgctgaca
    94021 tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc atttgttgga
    94081 cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga cgtttgcaag
    94141 tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc ccacatctga
    94201 ttgtcggggc gagaatgatt agccagcaaa acaagatcat agttctcctt tcttttttcc
    94261 gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc taaaaaaagt
    94321 gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg atctagataa
    94381 cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct tgatgccaac
    94441 ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt ctttgcattc
    94501 atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg aataagcgtt
    94561 gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat caaacgatca
    94621 aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc tattactgtc
    94681 ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt gatccaagaa
    94741 agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct gggaatccat
    94801 cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa ttggcgaaga
    94861 taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt catctgcatt
    94921 cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca gtattcataa
    94981 ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc catcataccg
    95041 tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt atcatttctg
    95101 aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt tacgtacctc
    95161 tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt aatgtaatag
    95221 ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg cttcgcctaa
    95281 ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt gaaccacttc
    95341 tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat cttctgctaa
    95401 gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga acccagtcac
    95461 tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca atcctacaac
    95521 accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag cgactttccc
    95581 aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat aataaaccgc
    95641 tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg cccgatttct
    95701 gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa cactttgcgg
    95761 ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc cattttttac
    95821 taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac gctcgatctt
    95881 ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat cctctttcta
    95941 taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct tggcaaataa
    96001 agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg gttttccaat
    96061 aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc aagttctgca
    96121 tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc aactaaaggg
    96181 atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt gtatttatca
    96241 gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag ttttgactca
    96301 cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt ttcatattct
    96361 tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc aaccgctgag
    96421 aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct accgccccat
    96481 tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata cgtatttgct
    96541 ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc tttgccaaat
    96601 gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc cgcatcagga
    96661 cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt tgctcgactt
    96721 gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc ttggattgct
    96781 ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag ttcacctggc
    96841 cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc aagaccttta
    96901 gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa aaagctgagt
    96961 tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga aggtaaagta
    97021 attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga ttaacctatc
    97081 gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt tgcttaaaaa
    97141 attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta attctttact
    97201 tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc taccatcgac
    97261 ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc gaccttcgct
    97321 aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa tcgttgatgg
    97381 ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt cttttaatat
    97441 caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt catcagctaa
    97501 tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat ctaacgcttt
    97561 ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt atgattttta
    97621 ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg aaaaaaagaa
    97681 gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg aacaccgtga
    97741 atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt gtgaaaatgg
    97801 ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt gaacaccgtt
    97861 tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc caaacaaaaa
    97921 tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga ttgtacaccg
    97981 tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca atttcaaaaa
    98041 acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc aggagaatac
    98101 tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt atttcctttt
    98161 attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat gattttttct
    98221 ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata tatccataac
    98281 gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac gacgtataac
    98341 caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc tgtttcaaat
    98401 agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct atagctccga
    98461 gtccgttttc agctatgaac agtggttttt gatacctatc atataattga ttcaaagcaa
    98521 ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga tacggatttt
    98581 taattcctcc agttattaag tttccttgga ccccttccca attttctgga tgagcactga
    98641 ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct gctaagattt
    98701 cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt cgttgcgtat
    98761 acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt ctttgctgct
    98821 caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc tgatagctga
    98881 tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta gctaaagcac
    98941 ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg ggatggtttt
    99001 cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc tcattgaaag
    99061 tcatccaata ttttacttt
//
LOCUS       DAZKCY010000002        69460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000002 DAZKCY010000000
VERSION     DAZKCY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.002"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..642
                     /gene="purE"
                     /locus_tag="ACG8XC_000087"
     CDS             154..642
                     /gene="purE"
                     /locus_tag="ACG8XC_000087"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP9189822.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            635..1759
                     /gene="purK"
                     /locus_tag="ACG8XC_000088"
     CDS             635..1759
                     /gene="purK"
                     /locus_tag="ACG8XC_000088"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP9189823.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            1772..3067
                     /gene="purB"
                     /locus_tag="ACG8XC_000089"
     CDS             1772..3067
                     /gene="purB"
                     /locus_tag="ACG8XC_000089"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP9189824.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3247..3624)
                     /locus_tag="ACG8XC_000090"
     CDS             complement(3247..3624)
                     /locus_tag="ACG8XC_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189825.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            3880..4608
                     /gene="purC"
                     /locus_tag="ACG8XC_000091"
     CDS             3880..4608
                     /gene="purC"
                     /locus_tag="ACG8XC_000091"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP9189826.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            4608..4862
                     /gene="purS"
                     /locus_tag="ACG8XC_000092"
     CDS             4608..4862
                     /gene="purS"
                     /locus_tag="ACG8XC_000092"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFP9189827.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            4864..5535
                     /gene="purQ"
                     /locus_tag="ACG8XC_000093"
     CDS             4864..5535
                     /gene="purQ"
                     /locus_tag="ACG8XC_000093"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFP9189828.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            5536..7758
                     /gene="purL"
                     /locus_tag="ACG8XC_000094"
     CDS             5536..7758
                     /gene="purL"
                     /locus_tag="ACG8XC_000094"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFP9189829.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            7743..9182
                     /gene="purF"
                     /locus_tag="ACG8XC_000095"
     CDS             7743..9182
                     /gene="purF"
                     /locus_tag="ACG8XC_000095"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP9189830.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            9205..10257
                     /gene="purM"
                     /locus_tag="ACG8XC_000096"
     CDS             9205..10257
                     /gene="purM"
                     /locus_tag="ACG8XC_000096"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP9189831.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            10254..10832
                     /gene="purN"
                     /locus_tag="ACG8XC_000097"
     CDS             10254..10832
                     /gene="purN"
                     /locus_tag="ACG8XC_000097"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP9189832.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            10829..12364
                     /gene="purH"
                     /locus_tag="ACG8XC_000098"
     CDS             10829..12364
                     /gene="purH"
                     /locus_tag="ACG8XC_000098"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP9189833.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            12380..13654
                     /gene="purD"
                     /locus_tag="ACG8XC_000099"
     CDS             12380..13654
                     /gene="purD"
                     /locus_tag="ACG8XC_000099"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP9189834.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            13776..14690
                     /locus_tag="ACG8XC_000100"
     CDS             13776..14690
                     /locus_tag="ACG8XC_000100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP9189835.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(14823..16388)
                     /locus_tag="ACG8XC_000101"
     CDS             complement(14823..16388)
                     /locus_tag="ACG8XC_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189836.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(16381..17832)
                     /locus_tag="ACG8XC_000102"
     CDS             complement(16381..17832)
                     /locus_tag="ACG8XC_000102"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9189837.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(17846..18862)
                     /locus_tag="ACG8XC_000103"
     CDS             complement(17846..18862)
                     /locus_tag="ACG8XC_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9189838.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            19082..20170
                     /gene="chvE"
                     /locus_tag="ACG8XC_000104"
     CDS             19082..20170
                     /gene="chvE"
                     /locus_tag="ACG8XC_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9189839.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            20224..21750
                     /gene="mmsA"
                     /locus_tag="ACG8XC_000105"
     CDS             20224..21750
                     /gene="mmsA"
                     /locus_tag="ACG8XC_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9189840.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            21752..22957
                     /gene="mmsB"
                     /locus_tag="ACG8XC_000106"
     CDS             21752..22957
                     /gene="mmsB"
                     /locus_tag="ACG8XC_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFP9189841.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            23214..24191
                     /gene="guaC"
                     /locus_tag="ACG8XC_000107"
     CDS             23214..24191
                     /gene="guaC"
                     /locus_tag="ACG8XC_000107"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP9189842.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            24455..25879
                     /locus_tag="ACG8XC_000108"
     CDS             24455..25879
                     /locus_tag="ACG8XC_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9189843.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(25921..26178)
                     /locus_tag="ACG8XC_000109"
     CDS             complement(25921..26178)
                     /locus_tag="ACG8XC_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189844.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            26340..27449
                     /gene="pdhA"
                     /locus_tag="ACG8XC_000110"
     CDS             26340..27449
                     /gene="pdhA"
                     /locus_tag="ACG8XC_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFP9189845.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            27452..28429
                     /locus_tag="ACG8XC_000111"
     CDS             27452..28429
                     /locus_tag="ACG8XC_000111"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFP9189846.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            28454..29749
                     /locus_tag="ACG8XC_000112"
     CDS             28454..29749
                     /locus_tag="ACG8XC_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFP9189847.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            29757..31163
                     /gene="lpdA"
                     /locus_tag="ACG8XC_000113"
     CDS             29757..31163
                     /gene="lpdA"
                     /locus_tag="ACG8XC_000113"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP9189848.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            31325..31600
                     /locus_tag="ACG8XC_000114"
     CDS             31325..31600
                     /locus_tag="ACG8XC_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFP9189849.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            31606..32373
                     /locus_tag="ACG8XC_000115"
     CDS             31606..32373
                     /locus_tag="ACG8XC_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFP9189850.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            32541..34370
                     /gene="typA"
                     /locus_tag="ACG8XC_000116"
     CDS             32541..34370
                     /gene="typA"
                     /locus_tag="ACG8XC_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFP9189851.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            34631..36403
                     /gene="recQ"
                     /locus_tag="ACG8XC_000117"
     CDS             34631..36403
                     /gene="recQ"
                     /locus_tag="ACG8XC_000117"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFP9189852.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLRKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            36517..36819
                     /locus_tag="ACG8XC_000118"
     CDS             36517..36819
                     /locus_tag="ACG8XC_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFP9189853.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            36822..37985
                     /locus_tag="ACG8XC_000119"
     CDS             36822..37985
                     /locus_tag="ACG8XC_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP9189854.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            38011..41439
                     /locus_tag="ACG8XC_000120"
     CDS             38011..41439
                     /locus_tag="ACG8XC_000120"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFP9189855.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            41460..42584
                     /locus_tag="ACG8XC_000121"
     CDS             41460..42584
                     /locus_tag="ACG8XC_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFP9189856.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            42565..43011
                     /locus_tag="ACG8XC_000122"
     CDS             42565..43011
                     /locus_tag="ACG8XC_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9189857.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            42995..43333
                     /locus_tag="ACG8XC_000123"
     CDS             42995..43333
                     /locus_tag="ACG8XC_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFP9189858.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            43452..44012
                     /gene="rsmD"
                     /locus_tag="ACG8XC_000124"
     CDS             43452..44012
                     /gene="rsmD"
                     /locus_tag="ACG8XC_000124"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFP9189859.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            44009..44500
                     /gene="coaD"
                     /locus_tag="ACG8XC_000125"
     CDS             44009..44500
                     /gene="coaD"
                     /locus_tag="ACG8XC_000125"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP9189860.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            44490..45533
                     /locus_tag="ACG8XC_000126"
     CDS             44490..45533
                     /locus_tag="ACG8XC_000126"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFP9189861.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            45692..46420
                     /locus_tag="ACG8XC_000127"
     CDS             45692..46420
                     /locus_tag="ACG8XC_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP9189862.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            46536..46781
                     /locus_tag="ACG8XC_000128"
     CDS             46536..46781
                     /locus_tag="ACG8XC_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9189863.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            46778..46996
                     /locus_tag="ACG8XC_000129"
     CDS             46778..46996
                     /locus_tag="ACG8XC_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9189864.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            47132..47671
                     /locus_tag="ACG8XC_000130"
     CDS             47132..47671
                     /locus_tag="ACG8XC_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFP9189865.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            47664..49937
                     /locus_tag="ACG8XC_000131"
     CDS             47664..49937
                     /locus_tag="ACG8XC_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFP9189866.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            49975..51021
                     /gene="holA"
                     /locus_tag="ACG8XC_000132"
     CDS             49975..51021
                     /gene="holA"
                     /locus_tag="ACG8XC_000132"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP9189867.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(51110..51364)
                     /gene="rpsT"
                     /locus_tag="ACG8XC_000133"
     CDS             complement(51110..51364)
                     /gene="rpsT"
                     /locus_tag="ACG8XC_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP9189868.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(51764..52033)
                     /locus_tag="ACG8XC_000134"
     CDS             complement(51764..52033)
                     /locus_tag="ACG8XC_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189869.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(52295..52423)
                     /locus_tag="ACG8XC_000135"
     CDS             complement(52295..52423)
                     /locus_tag="ACG8XC_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189870.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(52503..52958)
                     /locus_tag="ACG8XC_000136"
     CDS             complement(52503..52958)
                     /locus_tag="ACG8XC_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP9189871.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(53096..55024)
                     /locus_tag="ACG8XC_000137"
     CDS             complement(53096..55024)
                     /locus_tag="ACG8XC_000137"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9189872.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(55221..56054)
                     /locus_tag="ACG8XC_000138"
     CDS             complement(55221..56054)
                     /locus_tag="ACG8XC_000138"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9189873.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(56246..56950)
                     /locus_tag="ACG8XC_000139"
     CDS             complement(56246..56950)
                     /locus_tag="ACG8XC_000139"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9189874.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(56973..57599)
                     /locus_tag="ACG8XC_000140"
     CDS             complement(56973..57599)
                     /locus_tag="ACG8XC_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9189875.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            57925..60300
                     /locus_tag="ACG8XC_000141"
     CDS             57925..60300
                     /locus_tag="ACG8XC_000141"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP9189876.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(60324..60815)
                     /locus_tag="ACG8XC_000142"
     CDS             complement(60324..60815)
                     /locus_tag="ACG8XC_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFP9189877.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            61002..61922
                     /locus_tag="ACG8XC_000143"
     CDS             61002..61922
                     /locus_tag="ACG8XC_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9189878.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            62076..62420
                     /locus_tag="ACG8XC_000144"
     CDS             62076..62420
                     /locus_tag="ACG8XC_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFP9189879.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(62610..63110)
                     /locus_tag="ACG8XC_000145"
     CDS             complement(62610..63110)
                     /locus_tag="ACG8XC_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9189880.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            63638..63721
                     /locus_tag="ACG8XC_000146"
     tRNA            63638..63721
                     /locus_tag="ACG8XC_000146"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63672..63674,aa:Leu,seq:cag)
     gene            complement(64045..64668)
                     /locus_tag="ACG8XC_000147"
     CDS             complement(64045..64668)
                     /locus_tag="ACG8XC_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9189881.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            65003..65437
                     /locus_tag="ACG8XC_000148"
     CDS             65003..65437
                     /locus_tag="ACG8XC_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9189882.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            65460..65780
                     /locus_tag="ACG8XC_000149"
     CDS             65460..65780
                     /locus_tag="ACG8XC_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189883.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            65921..66112
                     /locus_tag="ACG8XC_000150"
     CDS             65921..66112
                     /locus_tag="ACG8XC_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189884.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(66183..66440)
                     /locus_tag="ACG8XC_000151"
     CDS             complement(66183..66440)
                     /locus_tag="ACG8XC_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFP9189885.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            66598..66861
                     /locus_tag="ACG8XC_000152"
     CDS             66598..66861
                     /locus_tag="ACG8XC_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189886.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            66885..67073
                     /locus_tag="ACG8XC_000153"
     CDS             66885..67073
                     /locus_tag="ACG8XC_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189887.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(67156..67341)
                     /locus_tag="ACG8XC_000154"
     CDS             complement(67156..67341)
                     /locus_tag="ACG8XC_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189888.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            67556..67927
                     /locus_tag="ACG8XC_000155"
     CDS             67556..67927
                     /locus_tag="ACG8XC_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9189889.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(68077..68149)
                     /locus_tag="ACG8XC_000156"
     tRNA            complement(68077..68149)
                     /locus_tag="ACG8XC_000156"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(68114..68116),aa:Gln,seq:ctg)
     gene            complement(68195..68623)
                     /locus_tag="ACG8XC_000157"
     CDS             complement(68195..68623)
                     /locus_tag="ACG8XC_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189890.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(68683..69441)
                     /locus_tag="ACG8XC_000158"
     CDS             complement(68683..69441)
                     /locus_tag="ACG8XC_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFP9189891.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT        22858 a        11923 c        14840 g        19839 t
ORIGIN      
        1 aggaatgtga cctaactttt gtcacattcc ttttttttat ctttaataaa cacgaacaat
       61 aaaatgtttt tctttgttaa cattcgaaat ttctttgaca tcaaaaaaag tgattgttaa
      121 aatatgaatg tagaaaaact gggaggaaaa acaatgacag caaaaatctc tgtgattatg
      181 gggagtactt cagattggga aacaatgaaa gaagcatgtc aaatattgga tgaattcggt
      241 gtgtcttatg aaaaaaaggt cgtttctgct catcgaaccc cagatttgat gtttgaatat
      301 gcacaaaacg ctcgaaaaaa aggaatcaaa atcattattg caggagcagg gggagccgcg
      361 catctgccgg gtatggtcgc atcaaaaaca actttgccgg ttatcggtgt accggtccaa
      421 tcacgaacat taaatggttt ggattcgctt ctttcgattg tccaaatgcc tggaggagta
      481 cctgtagcta ctacagcaat cggaaaagcg ggagcaatta atgctggact acttgcgata
      541 cagatgcttt ccatgtatga tttagaattg gaggctaaat tggctgaaag acgttcaatg
      601 ttggctaaaa ctgttataga aagtagtgat caacttggtt aaacctttgt tgcctggttc
      661 aacgatcggg atcgtcggtg gcggtcaatt aggtcgtatg atggcgatta gtgcaaaaga
      721 aatgggcttt aaagtaggtg tgctggatcc tgtgagcgat tgtccagccg cacaagttgc
      781 agattggcat attgaaagtg tctatgatga cacctttgcg ctgaaagagc ttgccagaag
      841 aacagatgtc attacctatg aattcgaaaa cgtcagtgta gaggcgttaa atgcgatttt
      901 acccatgtca tttattcctc aagggacaga cttgcttgcg attacgcaag accgcctgtt
      961 agaaaaatct tttttggaaa cgaataacat cgtgattgct ccttatgcaa cgatcgtcag
     1021 tccgacggat atccaagatg cgattgacgg gatcggctat ccttgtgttt tgaaaaccac
     1081 tcgaggcggc tatgatggca aggggcagta tgttttgaaa agtcgggcgg atctagctcc
     1141 tgccatggac cttttaagag aaggaacctg tgttttagaa gcatggatcc cgtttgaaaa
     1201 agagatttcg atcatggtgg caggaaatgg tcaaggagat ttcacgactt ttccggtcgt
     1261 ggaaaacatc caccacaaca atattcttca tgaaacgatt gctccagccg cgattgatca
     1321 ggatgtgatc gaggaggctg agcgtattgc ccgtgtgatt gcagaagcag tgagcctttc
     1381 aggtgtttta ggggtagaaa tgtttttaac aaaaacaggc gggctatatg tgaatgaatt
     1441 agctcctcgt cctcacaatt ctggacatta ttctattgaa gcttgttcta tgagtcagtt
     1501 cgatgcccat attcgaggta tttgtggttg gccattagga gaaaaagtac gtttgataag
     1561 caatgcagta atggtcaatg ttttgggaga acaactgctg gaaagctatc gcttgattga
     1621 gaataagcca gactggcaat tccatttcta tggaaaaaaa caagcaaaaa aaggtcgcaa
     1681 gatgggacat atcactatct tgacagaaga catccagcaa acgctggaag aaatcaaaca
     1741 aacgaatatt tgggattaga ggagaaaaaa catgttagaa cgttatacac gccctgaaat
     1801 ggggaatatc tggacggaag agaacaaata ccgtgcgtgg ctcgaagtcg agattttagc
     1861 agatgaagct tgggcagaac ttggtgaaat cccgcaagaa gatgtgaaaa aaattcgtga
     1921 gcatgcatca tttgatatcg atcggatctt ggaaatcgaa aaattaacac gccatgatgt
     1981 cgttgcgttt acacgagcgg tttctgaaac attgggagaa gaaagaaaat gggtccatta
     2041 tggtctaaca agtacggatg tcgtagatac tgcttatggc tatctcatca agcaagcaaa
     2101 tgatatttta cgggaagact tgaaacgttt tacggaaatc attggcgaaa aagcaaaaga
     2161 acacaaatat acagtgatga tgggtcggac acacggtgtc catgctgaac caactacttt
     2221 tggattgaaa cttgccacat ggtattcaga aatgaaacgg aacatcgaac gatttgaaca
     2281 tgcggcaaaa ggagtagaag caggaaaaat ctcaggagca gtgggaacgt tcgcaaatat
     2341 cccgccattt attgaagagt atgtatgtaa acaattgggg atccgtccac aagaaatttc
     2401 tactcaagtt ctccctcggg atctacatgc tgaatatttc tcagcgatgg cgcttattgc
     2461 tacaagtatt gaacgttttg caacagagat acgcggattg caaaaatccg aaacacgtga
     2521 agtagaagaa ttttttgcga aaggtcaaaa aggttcttct gctatgccgc acaaaaggaa
     2581 tccgatcggt tcagaaaata tggctggtct agccagagtg gtgcgcggac acatggtcac
     2641 tgcctatgaa aatgtcacat tatggcatga aagagatatt tctcattctt cagcagaacg
     2701 aatcatcttg cctgatacca cgattttatt gaattacatg ttaaatcgtt ttggcaatat
     2761 cgttaaaaat ctaacggtct ttcctgaaaa catgaagcgg aatatgtctg caacatttgg
     2821 tttgatttat agccagcgcg ttatgctgaa attgatcgat aagggaatga caagagaaga
     2881 agcttatgat cttgttcagc caaaaactgc gtacgcttgg gacaaccaag tgcagttccg
     2941 cccgctgtta gaagaagatc caaccatcac atcggtatta tctaaagaag aaatagacga
     3001 tgcctttgat tatcatcatc atttgaaaaa cgttgacgtg atattcaaac gagtaggttt
     3061 ggaataaaga agagtgagtt gcaaaattca attaaaatag aatagcaaaa ataaccaaaa
     3121 aagaagcaga tccgttagcg gttctgcttc ttttagttta ttatattttc ttatcaataa
     3181 taggaacctg attttaaaag cggctacctc tttaccgtgc ctctttacat acaaaaaata
     3241 gaggaattaa gcgtaaatgg cttggctatt ataactaaaa tgctggttat ggagatgttg
     3301 cagcaagttt tcgagaaaag cgtattccat ctcttgtcca ttgaataaga tctggatacg
     3361 ataatgattt ttgaaacgct tctgttcatg aatctgactg attgaaagtt cgcctttgtg
     3421 ttctgtctga taaagaaaag tccttttacg gcattcagca cagcgcttgg ttgctcggcg
     3481 gatttttcgg aatgtataaa aactagtatc tttattgcat aaataaacgt ctttgatcga
     3541 cagcaaatca ttatatagac aggaacgttc ttcagctgtt aattctgata aaaaaagctg
     3601 accttgtttt attgcatctt ccatcggcaa gtgcctcctc gaacccatta accaaatttt
     3661 tagttaattc taatatacca tcattaagta taaatgtaaa cgcttgttga tggtataaga
     3721 ttttcgtttc tgataaaaca cgaacattaa aagaatctaa tgccttaaat gtacatattt
     3781 ttgttgattt ataaaaagat gattgctaaa atttaaagtg aaaaaacgaa caaaaaacga
     3841 tgtatttgct tttaatgtaa ataaaaagga gagacgtaca tggagaagaa agaacttttg
     3901 tatgaaggaa aagccaaaaa aatatatctt acttctgatg agaacttgat ttggatagaa
     3961 tatttagatc aagcaactgc gttgaatggc gcgaggaaag accagataag tggaaaagga
     4021 gaattgaata atcaaatcac aagtgcaatt ttccgttatc tgactcaaag aggaataaaa
     4081 aatcatttta ttgaacaatt gtctaaaacc gaacaattag caaaaaaagt acaaattatc
     4141 cctctggaag tggttatccg aaatattgca gcaggaagtt tctcaaaacg tctaggtgtt
     4201 cccgaaggta aagaattcat caaaccaatc attgaattct actataagga tgaccagttg
     4261 gatgatccat ttatcaatga agagcatatc tatttcttac aacttgcaaa gccggaagaa
     4321 atcagtgagc tgaaagccca agcgttggct attaatgaaa ttttgaccga attatttgct
     4381 aaggtcggtt tggttttagt agatttcaag ctagaatttg gccggtcgaa tggacaaatt
     4441 ttgcttgcgg atgaagtaac accggatact tgccgattat gggatgaacg tacgaatgaa
     4501 catatggaca aagacgttta ccgcagagat ctaggtgatt tgatcccagt ctatcaagaa
     4561 gtatataacc gtctatcgga actaactgaa gaaaaggagc gagtatgatg tatttcgtaa
     4621 aagtatatgt aacctataaa gattcagtgt tagatcctca aggggaggca gtaaaaaaag
     4681 cagtccatcg tatgggatat gaaacaatcg aagatatccg aataggaaaa tactttgaga
     4741 tacaacttgc tgatgcagaa gaaccaatcg atacagtgat tgaaacgatt tgtgacaagc
     4801 tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat caaaaaaatg gaggaagtat
     4861 aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt gtgacgcaga tatgttatgg
     4921 gcagttcgcg agatcatggg cgctgatgcc gaatttgtac ggcatgatga gtcctcttta
     4981 gaaggatttg atggggtttt actgccggga ggattctcct atggtgacta tttgagatgc
     5041 ggtgcgattg ctcggttttc tacaatcatg gaagaagtga ttcgttttgc tgaagaaggg
     5101 aaaccagtat tcggtacatg caatggattc cagatattga cagaagcagg acttttgcca
     5161 ggtgtactga gacgaaatga gtctttgcat tttatttgca aaactgtacc attgaaagtc
     5221 aaccccaaga cggcctttac ttcagagtat gaagccgacg aaataatcta tcttccagtt
     5281 gctcatgggg aaggaaatta tttctgtgac gaagaaacac ttcaagaact aaaaaaaaat
     5341 aatcagattg cctttactta taatgacaac atcaacggca gcgtggaaaa cattgcaggg
     5401 attacgaaca aagcagggaa tgtgttagga atgatgcctc atccagaacg tgcagtagaa
     5461 gacttgctag gatcagaaga tgggaaacgg ttctttgctt ccattttaaa aaacttcgga
     5521 aaggtgaccg tatagatgaa aacattgaaa gaaccgacag ctgaagagat caagaaaaaa
     5581 agaatctatg ccgaatgggg attgactgat gaagaatatc gaatgatcga agaagatatc
     5641 ttaggaagaa tgcctaatta tacggaaact ggactgtttt ctgttatgtg gagcgaacat
     5701 tgttcctata aaaattctaa accggttcta cgcaaatttc ctactagtgg acctcatgtg
     5761 ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg gtgatggact agcagttgtc
     5821 ttcaaagcag aaagtcacaa tcatccttca gccgtggagc cttatgaagg agctgcgaca
     5881 ggagttggcg gaatcatcag agatattttc agcatggggg ctcgtccaat tgcgttgcta
     5941 gattcattga ggttcggcga attagataat ccaagaacga aatatttact agaagaagta
     6001 gttgcaggta tcggcggata cggcaattgt atcggcatac cgactgtagg cggcgaaatt
     6061 gcatttgatc cttgttatga aggaaaccca ttagtcaatg caatgtgcgt aggtttgatt
     6121 gaacacaaac atatccagaa agggcaagcc gctggcgtcg gtaattcgat catgtatgta
     6181 ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt ttgcctcaga agaatttaat
     6241 caagaagaag aacagcaacg ttctgctgta caagtaggcg atccttttat ggaaaagctg
     6301 ctgcttgaag cgtgtttgga attaatcttg catcattcgg attctctaat aggtatacag
     6361 gatatgggag cagcaggact tgtttcatct agtgcggaaa tggcttcaaa agctggctca
     6421 ggacttatat tgacattaga tgaagtccca caaagagaaa ctgggatgac accttatgaa
     6481 atgatgttat cggaatcaca agaacggatg ttgatttgtg ttaaatcagg agaggaagaa
     6541 aaaatccaag aactatttca gaagtatgat ctagatgcag tcactatcgg taaagtaaca
     6601 gacgacgggc aatatcggct atatcatcaa ggagaagaag tagcaaatct gccagtcgat
     6661 gcattagctg aagatgcacc agtttatcat aaagaaatga aagaaccagc tcgaatcgta
     6721 gaatttcagc agttagctcc ttatcagcca gttatcgaag aacctggaca agtattgctt
     6781 gatctgctgc agcaaccgac gattgcttca aaacgttcaa tctacgaaac ttatgattca
     6841 caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg ctgctgtgat gagggttaga
     6901 gggacgaata aagcgttagc tatgaccaca gactgcaacg cgcgctatct gtatttggat
     6961 ccaaaaatcg gaggacaaat agcagtggca gaagctgcta gaaatattat tgctagcggt
     7021 ggtaagccct tggcgatcac cgactgcctg aactatggtt ctcctgataa accagaagtt
     7081 ttttgggaat tatcgacttc agctgacgga attgctgctg cgtgtgaaac gttaggaact
     7141 cctgttattt caggaaatgt ctctttatat aatgaaacgg atggtcaagc aatctatcca
     7201 acaccaatga ttggtatggt cggattgatt gaggatcata aacatatcac tactcaggaa
     7261 ttcaaaaaat caggggatct aatctatata ttaggtaaga cgtttgcaga ttttgatggc
     7321 agtgaattac aaaagatgca gcttggaaga atcgaaggtg tgatccgaaa ctttgatcta
     7381 tccattgaaa agagaaacca agagttagta ttgacagcca tccaaaacgg attgattgaa
     7441 agtgctcatg actgttcaga aggtggcttg gcaattgcac tggcagaatc agctttcaaa
     7501 catcagctag ggatatctgt acagtttgaa ttgtcctctg ctcaattatt tgcagaaact
     7561 caatcaagat ttgtgttgac agtcgcaccg gaaaataaga cacgattcga agaaatgatg
     7621 ggagatgctg ctgttttagc aggtaaggtg acggatgaag cgataatcga aatttcagca
     7681 actgacggtc agatcaaaat agaaactgca gtagcaagaa agtgctggga ggatgcaatc
     7741 gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg gatcttcggt gtttggggtc
     7801 atccagacgc cgctagtgtg gcatacttcg ggctccatag tcttcagcat cgaggacaag
     7861 aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa ggggcatcga gatttggggc
     7921 tgctttctga ggtattcaaa gatcaaagaa aattgcagca gttaacagga gaagctgcaa
     7981 tcggacatgt acgttatgca acagcaggca atggaagtgt ggacaatatt cagccttttc
     8041 tattcaaatt tcatgatcag tcgatcggct tagcgcataa tggtaacttg atcaatgctc
     8101 gttcccttag aaaagagtta gaggaaaatg gggcgatctt ccattctaac tccgatacgg
     8161 agatattgat gcatttgatc cgacgaagtg aagaaacaga ttttttaaat aaattaaaaa
     8221 gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt aatgacagaa acggcaatga
     8281 ttgccgcttt ggacccaaat ggttttcgtc cattagcaat cggacaaatg aaaaacggag
     8341 catacgtgat tgcctcggaa acttgtgcat tagaagttat tggggcacgt tttataagag
     8401 atgtccagcc tggtgagata gtgatcatca atgacgaagg aatccagatt gattgcttta
     8461 cgaaggacac gcagctttct atttgttcga tggaatatat ctattttgcc agaccagatt
     8521 caaacatcgc agggatcaat gtgcatactg cacggaaaaa tatgggacgg aatttagcga
     8581 aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc taattcttct ttgtcagctg
     8641 caagcggtta tgcggaagct agtggaatcc cttatgaaat cggattagta aaaaatcaat
     8701 acattgcccg aacatttatc cagccaacgc ctgagctaag agagcaaggt gttcgaatga
     8761 aattatctgc tgtacgagga gtagtggaag gtaaaaaggt gattatggtc gatgattcaa
     8821 ttgttcgggg tacgacaagc agacggatcg tccatctttt gaaagaagca ggggcaaaag
     8881 aagtccatgt gaggattgct tctcctcctt tgaaatatcc atgtttttac gggattgata
     8941 ttcaaacgcg taaagaactc attgcggcaa atcacacgat tgatgaaatc aaagaatgta
     9001 tcggtgcaga ttctttaggt tttttaagtg aagagggtct gatcgaatcg atcggtctgg
     9061 aaaaagatgc tccgtattca ggattgtgta tggcttattt caacggagat tatccgacgc
     9121 cgttatatga ctatgaagaa aaatatcagg aatcactaaa agaaaaagta tcgttctttt
     9181 aaaagcagac caagaaggga gtatatgaat tcaatggcaa atgcatacgc aaaagctggc
     9241 gtagatgtcg aagctggata tgaagtagta gaacggatca agaagcatgt caaaaaaacg
     9301 gaacgtttag gtatgatggg cgctatagga ggattcggcg gttgttttga tttatcggtc
     9361 tttcaattaa aagagccagt ccttgtctcg gggaccgatg gcgtggggac gaaattagca
     9421 gtggcaatcg aagcagacaa acacgataca atcgggatcg actgcgtagc gatgtgtgtc
     9481 aatgatatct tagcacaagg cgcagagccg ttatatttct tagactactt agcaacgggg
     9541 aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg tagcagatgg atgtgttcaa
     9601 ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc ctggtatgta ctcaggtaat
     9661 gattatgatc tagccggatt tgctgtaggg atagctgaaa aatctcaatt attgacagga
     9721 gaaacgatca acccaggaga tgtgttgctt ggaatagcat ctagcgggat tcattcaaat
     9781 gggtactctc ttgttcgcaa aattttcttt gagcagcagc agttttcttt atcatcaaaa
     9841 ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg agccaacgaa aatctatgtt
     9901 tcttccttgc ttccgctgat caaagaaaag aaaataaaag gagctgcaca tatcactggt
     9961 ggagggtttg tagaaaatat cccgcgaatg ttaccagaat ctatggctgc acaaatcaaa
    10021 cttggctcat ggccgattct tccaatattc ggctgtttac aaaaatatgg tgaaattcct
    10081 gagatggaga tgtatgaaat cttcaatatg ggcatcggta tggtcctggc agtcgaagaa
    10141 tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg gcgaaaaagt ttatgaaatc
    10201 gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta taggagaaga acgatgagaa
    10261 tcgctgtttt tgcttctgga aacggaagca actttcaagc gctagccgat tatctatcga
    10321 aaaaaggatt ggaatcttcg attgactggt tgttttgtga tcagcctgaa gcatatgttt
    10381 tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt ttcacctaaa gaatttgatt
    10441 caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa agaaaaaaag atcgatttga
    10501 tcgtattagc tggatatatg cgaatcattg gaccggtatt gctgaaaaat tatgataaaa
    10561 gaatcatcaa tatccatccg tctcttttac ctgccttccc tggtttgcat ggcatacgtg
    10621 atgcctttga agctggagta aaagaaacgg gtgtcacaat tcattatatc gaccaaggtg
    10681 tagatactgg accaatcatc cggcaggaaa aagtgagaat tgaacaggag gacacgttcg
    10741 attctttaga agaaaaaatc catagagtag agcatcgaat ctatccagaa gtgattagtg
    10801 aaattattga aaatggaggc tatctgaaat gacaagagca ttgatcagtg tatcagataa
    10861 aactgggatt gtagcattcg cccaaggttt gattgaacaa ggagtagaaa tcatttcaac
    10921 aggaggaaca aagcatgcgt tggataaagc tgggatcaaa acgctttcga tcgaagaagt
    10981 gacagggttt cctgaaatga tggatggccg tgtcaaaaca cttcatccta aaatccatgg
    11041 tggtctttta ggaagacgcg atctccctag ccatatggag gcaatggaaa cggaaaatat
    11101 ccaaccaatc gactttgttt gtgtcaattt atatcctttc aaagaaacga ttatgaaacc
    11161 agatgtcgaa atagcagaag ctattgaaaa cattgatatc ggtggtccta gtatgctccg
    11221 tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt gatccatctg actatgaaga
    11281 agtactttca gaattacgtc agtatgggca tacttcactt tctttcagac aaaaactagc
    11341 ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg atcgctcaat acttaacgga
    11401 ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg tatgaacgaa aacaaacgct
    11461 tcgttacggt gaaaatagcc atcaagccgc tgccttttat cagtccgctc ttctggaaaa
    11521 atattcgatc gcagcagcaa aacaactcca tgggaaagaa ctttcttata ataatatccg
    11581 agatgcagat gctgcgctac ggatcatgaa ggaatatgac gagccaacgg tagtcgcctt
    11641 gaaacacatg aatccttgtg gtattgggac agcatctacg attttagaag cgtgggaagc
    11701 agcgtatgaa gcagatcctg tttcaatctt tggtgggatc atcgttttga atcgagaagt
    11761 agatctgcca acagcagaac agatgcataa gctgtttcta gaaatcatca ttgccccttc
    11821 ttatacacaa gaagctttgg aattactgca aaaaaagaaa aacatccgat tactgacagt
    11881 agactttgcg caggaagaaa atcaaaatcg agaagaaacg gtctcagtct taggtggact
    11941 tcttgtacaa gatcaagatc tggcaataga aaatgaagaa gagtggaaag tcgtgacaaa
    12001 acgtaagccg actccagcag aagcaaaagc attagtgttt gcttggagag ctgtcaaaca
    12061 tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag acagtaggaa tcggagccgg
    12121 gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa caagcgcaag agaaaatgga
    12181 aggtgcagtt ttagctagcg atgcttattt tccaatggat gattcggttg aatatgcagc
    12241 caaacatggt ataaaagcaa tcattcagcc aggaggaagc atcaaagacc aagattctat
    12301 tgatatggcg aataaatacg atctagcaat ggtctttact gatgttcggc attttagaca
    12361 ttagagagga gtcggataaa tgaacatttt ggtgatcggt gcaggtggtc gtgaacatgc
    12421 aataagtaaa aaacttttag ccagtccgaa ggtggatacg gtgtattgtg cgccaggaaa
    12481 tccggggatg acaaaagatg gtatcaattg tgtcgctatc agtgagcgag atcattcagc
    12541 gttaatccat tttgttaaga aagaggctat tgattggagt atcgttggtc ctgaagttcc
    12601 tttgttaaat gggatcgtcg atgattttat ggcggaagga ttgaaaatct tcggtccgac
    12661 aaaagcagca gctctgatcg aaggttcgaa aagttttgct aaacaattaa tggatactta
    12721 tcaaatcccg acagctaaat ccagaagttt tgaatgttac gaaaaagcag cagcgtatat
    12781 caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc ttagcagctg ggaaaggtgt
    12841 agtcgtggct accacagtga aggaagcgct ggatgcggca gaacaaatgt tgaaccagca
    12901 tcgatttggg gatagtagca aaaaaatcgt cgtagaagag tttttggcag gagaagaatt
    12961 ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg atggtcattt cgcaagatca
    13021 taagcgggca tttgataacg ataaagggcc aaatacaggc ggaatgggtg cttattcacc
    13081 agtaccgcaa atttcaaaaa agatgattga aaaagctgtg gaagaagttt taaaaccagc
    13141 ggctttggga atgaaagaac taggacgctc tttcacgggt gttttatatg cgggtttgat
    13201 tgctacgaag gaaggaccaa aagtgatcga attcaatgcg cgtttcggtg atcctgaaac
    13261 gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa gtaatcgatg atttattaga
    13321 gaatcgtaca gtagagttaa agtggcagca agaaggatac aatgtaggcg ttgtggttgc
    13381 agctgccggt taccccgaag aatatgagac aggaatggtt ctaccagatt ttctggaaga
    13441 agagctttct gtttactatg ctggagtttc ctcaaataac ggcaaactag ttggagcagg
    13501 aggacgtctt tatttagtag aagcatacgg tgagacgatc aaagaagcac agaaaaaaat
    13561 ttacaccgcg ttaatggata aaacgacaga tggaacattt tatcgtacag atattggaga
    13621 aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc tgcttccatt tataaaaaat
    13681 acaacaaaca taatcggaag cgccgatttt agtgaagcct tttcataaag agtgtggtac
    13741 acttttgttg tgtatataaa taaggaggag ttcagatgaa aaaaattcaa ttaggtactt
    13801 ctgatcagaa agcagctgca gtgatcttag gctgtatgcg tatcaacaat gcggaagatc
    13861 cagtaaaagt gattgagaca gcttatgaaa acggcattga tttttttgac catgcagata
    13921 tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc tctagcaaaa acatcaatca
    13981 aacgagaaga tctttttatt caaacaaaat gtggaatcgt tccaggtgtg atgtttgatt
    14041 tttctaaaaa acatatcatt gaatctgtag aaaaaagttt gacccgtctg aaaatggatt
    14101 atgtggatgc tttacttctt catcgtccag atacattggt agaaccagag gaagtagctg
    14161 aagcgtttga tgaattggaa cggtcaggaa aagttcgtta ttttggcgtc agcaatcaaa
    14221 aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca accattagta gctaatcagc
    14281 tgcaatttgg cttgaaacat actggaatga tcgatcaagg gattcatgtc aatatgacag
    14341 atgaagcaag tatcgatcat gacggtagtg tacttgatta ttcccgattg catgatatga
    14401 cgatccaagc atggtcacca taccagtatg gattttttga aggtgtattt atcgggaacg
    14461 aaaaattccc tgaattaaac caaacattag aagaactagc agctaaatat caaacgacac
    14521 caactggctt ggcttctgct tggattttac gccatccagc caatatgcaa gtgatcgccg
    14581 gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc ttcagaaatt aatatgtctc
    14641 gggaagattg gtacgaattg taccgtgctg ctggaaacat tttaccttaa agattgtcat
    14701 aagcaggtca aaagggctat aaattaaaaa gacagaataa cttgaaagct gttttcagct
    14761 tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt atcattgtac ggatttcgtt
    14821 tattagttat ttttgttttt aaggaaaagc tgtgtccgat attcttttgg agaaatccct
    14881 tgttttttct taaataaata actgaaatag ttagggtcat tataaccaat ttcaaaagca
    14941 atatctgaaa tacgatagtc tgattgctgc aataattttt ttgctaattc gattctttgt
    15001 tctgtcagat aatcgatgaa tgtttggtta aacgattgag aaaaaatcgt actgaaatga
    15061 gagggactta atgcaacttg ttgagcgacc caagttaagg acatatctgg atcggtaaaa
    15121 ttttctttga taaaatccaa tgcatggttc aaaattggct gatatttgtt ccttgaaggc
    15181 tgtgagggca gagcagttaa gaaagaaatg aacatataaa ctgtcgaacg gtaaagcgag
    15241 tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc aatgaaccat ttcttgcggg
    15301 attttctttt ctttcaggca gacatgctgg agtagcttag tcaagacaaa taaacgatat
    15361 aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt gttcgatgta cttagatact
    15421 tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa agtaaaagac agaatcaact
    15481 ggaagtttcg cttcttgaat caaatcctca tagctgataa tttgttcttt aagggaactg
    15541 gcggtttcaa gtatcatctg cgtgatctca aaagatgcag gtaattcgct gattctttct
    15601 gcgacaggac caatggcgac agaaaaatta tttttttgtc ccttttctaa ttcatggacc
    15661 aacgtttggg ctacctgata actggtctcg atcacatttt cttcaagaga atcaactatt
    15721 aaaaatttga tgaatcgaga ggaaacagca gagaagatca cttgttgatt ctccctaaat
    15781 aaaaacttca aataatcact gagatgagcg taatcttcaa agtctgtact gacttggttt
    15841 gctgctaata atacggtcat ttttttccct gctaaagagc gattgaaaga ttcagcaagt
    15901 ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac cgttcaagaa atgatttttt
    15961 ttcaattcga aaacaagatc agaagaagtg tgcgtttctt tttgctgcaa atctaaagca
    16021 gttactgctt tttctattgc ttgtttcaat tcgctacttt taatcggttt aagcagataa
    16081 tcatctgcct gtacctggat tgctcctttc atataatcga aatcgtcgaa tccactaata
    16141 aatatgagac gagtccaagg aagtagtttc cttgcttctt ttgaaaaagt caacccgtcc
    16201 atgaaaggca tttggatatc agtcagaatg atgtcgggct gtaagtcgat aattgaagac
    16261 agtgccatct caccatctcc agcttcgccg acataagtga ttggataata agaaccaagg
    16321 acagttattt gttcccgcaa gttattgcga atgaggtatt cgtcttcgat gataaaaact
    16381 ttatacatgt ttgtttcctc caattgtttt gggagataga gggatcatca gtgtgacagt
    16441 tgttccttct cggtatttgc tgtcaatcgt gataggtttt tgatcatttc cgtaataaag
    16501 aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata ctgaaatctg aatcgatccc
    16561 tttggctaac tctttacgta tttctgccag tttttctgca gggatgccaa tcccgttgtc
    16621 tataactttg aattggatca tctcatcaat tttgcatgtg gtaatacgaa taaatccttt
    16681 gtgacggacg tacttcgttc catggtatac agcattttca atcaatggct gcaagatcat
    16741 ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg atcttatagt caagcatatg
    16801 gccgtatctt atttttaaga tgactagata atcattgata tggctaattt ctttttcaag
    16861 aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa aagccggaaa gtgcatgggt
    16921 catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa gaaataatgg catccaaact
    16981 gttataaata aaatggggag taatctgagc ttgcaataca cgaacttcac tttgtgctaa
    17041 atggtattgt ttcaaagcgt tctcccgtaa agactggttt agatcttccg ccattttatt
    17101 catgctagcg gtaagtgttt gtaattccag tgtttccggc agggttaaac gaaagccaag
    17161 gttccctgaa gaaagttgtt ctgacatctc gatcagctga ttgatcggtt cttggacctt
    17221 tctctctaaa aagcgacgat tctttttcgc atagaaaaaa atggtactaa gaactccaag
    17281 ttccactatt gttaatagaa tgagcgagcg gacaagtgtc gtattttttt tgcttgctaa
    17341 gtcgatctcc acacgaacaa aatcttgtaa gatatctgac agcagttgag tgacagaatc
    17401 tacctgattg atgatctctt cattggtatc cataaaatct tttgccaaga tattattgat
    17461 cagctcgttt tgataatctt ccagtgtatc gatcgtacgt agcgatacat ctaacgtact
    17521 aatttcttta agtgtatttg tattattttt gctctctcga atatcattcc taagttcttc
    17581 taggatattt tcctcattga actcatctac ggttacttgg ccagaaatca agtcccacat
    17641 actttccaaa actttttgat caaccttcaa agcaattgca tctgcttttt ggatatttgc
    17701 gatcgtctgt tgataaatca aaagctgttg cgaatataac agtatgctca gtagcaaagg
    17761 gataacagca aatagaagca tggctttgtt tgcttgggca aaaatctgtt ttatgcttaa
    17821 attttttgtc atgatacttc tccagtcaat aatttctggt tgggatgtta gctatattct
    17881 gctgggaaaa gacagtctcg gaaataaaaa tatctttagg taatgttttt cctgtttgaa
    17941 tatttccaaa ataacgagta acggtattgg ctacatacca gccgacatca ggtttacact
    18001 cgactacaca attgacgatt cctgagcgta gcttctcgat catatcttct tgtccatcaa
    18061 tagttacaat gatcaaatct tctccaggtt tgattgatgt ttcttttatc gcagctaaag
    18121 caccgtgagt catttcatca ctatggctgt ataatacatc gatttcggat aaatcatttg
    18181 tttctaaata tcgtttcatc tggtcttttc ctttcatccg aataaaatca ccgacaattc
    18241 ggtgaacaat agtcatcgga ggtttatggt cgatggttcc atcttttttt agggttggtt
    18301 tacgattgat tgattccaaa aatccttcgg agcgtagctt ggtcggtgtg gagttctctg
    18361 aaccagcaag ttctaggatt ctgatttcct gtttctttga atttgtgaag tgattgctga
    18421 caaataaacc cgcccgattt ccttctgctt tgaaactggg gccaatgtgg gtgacgaaaa
    18481 gctcgggatc attcgtttga atatgtctgt cgacaagtat gacagggatc cctgctcgct
    18541 ttgcttcttt taatacagag tcccacccgg attcttgcaa aggagtaaaa atgatcatat
    18601 ccactttata ggcaatgaac gtccgtatat cttggatttg ccgttcctga ttcatgtagc
    18661 catttcggta gagaacttgg taatcttctt tttcaagcgc agtgataatt gattcagtat
    18721 gctttttccg ccagctactc tccgttcctg attgcgaaaa accaacgact agtgggtcag
    18781 gtgcttcctt tgtattaggt gtacacccca tcatttggta agcgattaca acaagggcta
    18841 aaaaaaataa aacacgtttc actttaaatc actccatctg gatttgttag tactatctta
    18901 tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg aaaaaaatcc aagaaactta
    18961 aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa agtaaatccc ataaaaaaat
    19021 caatgttaac gctttcttta ttagctatac tcggtgtatc aattgaaatg ggggataaaa
    19081 aatgaaaaac aaatttagaa agattgttgg gactatcgct attttgaccg caagcattct
    19141 attaagtgcg tgtggcaatt caggatctgc agatgatagt acggggtatg taggaatcgc
    19201 tatgccgacc aaatcagcag aaagatggat cgctgatggg aacaacatgg tctcggaatt
    19261 agaaaaactg ggttataaaa cagatcttca atatggagaa gataaagtag aaaatcaagt
    19321 agcacaaata gaaaatatga ttacaaaagg ggtagataca cttgtgattg cttctatcga
    19381 tggttcggct ttaactgatg tgttagctaa agcaaaagaa gcagatatca aggtgatcgc
    19441 ctatgatcgt ttactgatga attcagaaaa tgtcgattat tacgcgactt ttgataattt
    19501 tggagtaggt gtatcgcaag cagcttatat tgaagagcac ttaggattga aagaaggaaa
    19561 aggaccattc acgattgaat tatttggtgg ctcgcctgac gacaacaacg cactaatcaa
    19621 ttacaacgga gtgatgtctg ttttacaacc atatatggac aacggtcagt tggtcgtgcc
    19681 atctggacag acgagtttca gtcaaatcgc tacgcttcgc tgggatggtt ctacggcgca
    19741 agcaaggatg gataatctgt taagcgcaaa ttatactgac caaacattgg atgctgtttt
    19801 gtcgccatat gatccaataa gtttaggaat catttcgtca ttaaaaggcg tggggtatgg
    19861 gagtgaaagt aaacctttac ctgtgattac tggacaagat gcgacagttg ctggcgttaa
    19921 atcgatcatt gccggggaac aaacacaaac gatctttaaa gacacacgta tcttagcaaa
    19981 aaatacgatt gaaatgatca aagcaatcag tgatggagaa gaggttccgg tcaatgatac
    20041 tgaaacttat gataatgggg tgaaaactgt ccctacgtat ttagcaaata cagtatctgt
    20101 cgataaagat aattatcaag cagagctgat cgacaccgat tattataaag aatccgatct
    20161 gaaaaattaa gaggaaccaa acaaggaact aaagagatct aagtcagaga gaggataaga
    20221 attatggcgg attgtatttt aaaaatggca gatatcacca aaaaattttc tggaatccga
    20281 gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa tccatgcatt gtgcggagaa
    20341 aacggagcgg gaaaatcaac attgatgaat gtcttgagcg gcttgtatcc atatggctcc
    20401 tatgacgggg aaatctttta tgatggaaag ctgtgtcaat ttaaagattt gaaagatagt
    20461 gaagaaaaag gcatcgtcat cattcatcaa gaattagcac taagtcctta tctttcaata
    20521 aaagagaata tttttctcgg aaatgaacaa gcagaaagag gcgtgattga ttgggaactg
    20581 acagaaaaga aaacatatga actgctgaat aaagtcggtt tgtctatcga cccgaatctt
    20641 ctagtatccc agattggcgt agggcaacag caactagttg aaatagctaa agcattatca
    20701 aaatccgtcc gtctgttgat tttggatgag cctaccgctg cattgaatga agaagaaagc
    20761 gcgaatttgc tgagattgat ccgagagttg aaagagcaag gtgtaacctc catcattatt
    20821 tcacataagc tgaatgaaat cgtggatatc gcagatcgaa tcaccatttt acgggatgga
    20881 cagacgattg agacactaga aaatgaaatg atcagcgaag agcatattat tcgcggaatg
    20941 gtgggcagag atttagttga tcgttatcct gaaagaaaac cggatattgg agaagtttat
    21001 tttaaagtag aagactggac ggtctatcac cctcttgatc acgagcgaat cataaacgac
    21061 cacatcaact tgcatttgaa aaaaggagag attgtaggaa tagctggact gatgggagcg
    21121 ggcagaactg aattcgctat gagtctattt ggacattctt atggaagcaa gatatccgga
    21181 cgtatattaa aggaaggggc agagatatca gttaagaccg ttccttcagc cattgcaaac
    21241 ggactcgctt atgtctcgga ggaccgaaaa tctttaggac tgaatctgct gatggatatt
    21301 cgggaaaacg tgacgctgag cagtttggat aaaataagtc aaaaaggagt attagataaa
    21361 gaaaaagaag taactgaggc agaaaatttc cggaaaaaga tgcgtataaa aacaaactcc
    21421 atccatcaaa ttgtcagtag tcttagtggt gggaatcaac aaaaagtcgt tttatccaag
    21481 tggctgatga cacaacccga tattcttttt ttggatgagc caacccgtgg gatagatgtt
    21541 ggggcaaaat atgagatcta cacgattatc gaagaaatgg cagcaaatgg caaagctgtc
    21601 tgtcttatct cttctgaact tcctgaaata ttagggatgt gtgatcgaat ctatacgatg
    21661 aacgaaggac gtattacagg agaagtctcg cgagaagatg cagatcagga agtcttgatg
    21721 aaattaatga cgaaagaaga ggtgggttaa gttggaacag gcaaaaaatc ttacggataa
    21781 aaaagagaaa aaactaaatt tgaaaagtag agcagctgat ctttttggtc gctacagcat
    21841 ggtgatcatt ttagcgggac tacttattgc gtttcaattg atgacggatg gtattttctg
    21901 gcgtccattg aatataacta accttgttct gcaaaatagc catatattag tactagctgc
    21961 agggatgtta ttagttgtgc tcttaggtca tgtggatttg tcggtagggt cagtgatggc
    22021 gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc caaatgaacc cgtggatgac
    22081 agttatcgca tgtcttgcag taggcgggct gatcggcgca tggcaaggtt tttgggtagc
    22141 ttatgcaggg ataccggcgt ttatcgtgac tttagcagga ttgctgatgt ttcgtggact
    22201 gacacaagtt gttctaggag gccaatctct agcacctttt ccagctgttt tccaaaaaat
    22261 gtctacgggt tatttaccag acttaagcga aggaagcagc cttcacttct tgacattgat
    22321 tttaggaatg atcatcacga tcgtgttagt tgttggtcaa tggcgaacaa gaaatcgtcg
    22381 gaaaaatcat ttatttgaag tggaatctcc aacagctttc ttggtaaagg cggtatttac
    22441 agcagtcgtt gtactaggaa taacttatat ctttgcctct tatcaagggt atccgattat
    22501 tttgatcatt cttggtttga tcgttgcagc atatgggttt ttgacaaata aaacagtagc
    22561 aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg gctgaactat cagggatcaa
    22621 aacaaagcga atcacgttct gggtattcgt caacatggga gtcatggcag ctttagctgg
    22681 attagtttta gctgctcgat tgaatgcggc aacacctcaa gcaggtacat cgatggaact
    22741 ggatgcaatg gcggcagtat attttggtgg tgcttcgact tccggcggca ttggaacaat
    22801 catgggtgcg attgtcggtg gcttggtcat gggtgtattg aacaatggca tgtcgatttt
    22861 aggcgtcggg gtagactggc agcaggcaat caaaggattg atcttgcttt tagccgttgt
    22921 gctagacttg tataataaga aaaaacgaac aacgtaggag aacgccggtt attcagaaaa
    22981 aagactgtga caagacattt gtcacagtct ttttttcgct aaaagcgaac attcattgtt
    23041 tcgtttgtat agactaaaaa taatatatgt ttttttacca ttcgtataaa ttctcttttt
    23101 gctcttgatt tttttgctta attgagttaa attacatagg gcgctaaaaa aagccttgat
    23161 taaattaata atgttcgtgt tttgctggat gtgaacatca ggaggataat gtaatgaaag
    23221 tatttgatta cgaagatatt caattaattc ctaataaatg tatcgtaaat agccgttctg
    23281 aatgtgatac aacggtcaca ttaggtaaac atacattcaa aatgccagta gtgcctgcca
    23341 acatgcagac gattattgat gaaacaatcg cagaattctt agcggaaaat ggttatttct
    23401 atatcatgca tcggtttgat gaagcagctc gaattccgtt tatcaaaaaa atgaaaaagc
    23461 gtggattgat tacctcgatc agtgttggcg tcaagaaaga ggaatattcc tttatagaga
    23521 agttggcaga agaatcgcta aatcctgatt atatcactat cgacatcgcg catggacatg
    23581 ctaactcagt tattgatatg attcagcata ttaaaaaata tttgcctgaa acttttgtga
    23641 ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt ggaaaatgcg ggcgcagatg
    23701 caacaaaagt cgggattggt ccaggaaaag tatgtattac aaaaatcaag acaggctttg
    23761 ggactggagg ctggcaactg gcagctctta gatggtgtgc gaaagcagct cgcaagccga
    23821 tcatcgctga cggtgggatc agaacacatg gcgatattgc aaaatctgtc cgttttggtg
    23881 cgacaatggt catgattggt tcattgtttg ccggtcacga ggaatctcct ggcgaaacaa
    23941 aagtcgaaaa tggaatcgtg tataaagaat actttggttc tgcttcagaa ttccaaaaag
    24001 gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca acacaaagga tctttgaaag
    24061 atacactagt ggaaatgcaa caagaccttc aatcatctat ttcttatgct ggaggaagag
    24121 atctggaagc cattcgaaaa gttgactatg tcatcgtgaa aaattcgatt tttaatggag
    24181 acacaatcta aaaaataaat aaaatattag gatgttgtga aaaagttgtc ctgcattaag
    24241 taataagaac agccaaacaa aaatagttct ttatatgttt tgtttggctg gaattattat
    24301 cgcagataca agcttttgaa catcattatt cagaataagg gactgtgata agatttttgt
    24361 cacagtccct tatgttttgc aaaaaaagtt tttctttttt ctcgtaaatt cggtaaaatc
    24421 aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg gaaaaagtag agaaaaaaca
    24481 aggaccgatt tatcgtcaaa ttatggaaca gattatgaga gaaatcgaaa acggaaggat
    24541 caatcctgga agtcaacttg aatcagaacg aaaactaggg caactttttg gtgtgaatcg
    24601 ttccaccgtt gtccatgcat tggaagagct tagggagctt ggggtactaa cgagcaaacg
    24661 tggcagcgga tggtttgtta atcagactga atgggggaaa ttcgctgttc caagaatcga
    24721 ttggcggcaa atgatctctc ctcgttatga gcagacggac tggtatgagc aaaaaataag
    24781 agaagcaaag cagatccaaa aaacatcgtt tcttgattta tacgctagcg agatgccaga
    24841 tgagcttttg ccgaattttg agtttccttc ttattctctt gaagaaatct tagcggaaga
    24901 aaaagaaatg gatcttttag gctatactcc gttacgtaac aaaatcaaac ggtatttgga
    24961 aagtaaattt gaattggcat tttcccccaa ccagcttctt gtgactggtg gcggtcagca
    25021 agcaatcttt ttgattttac aaacgattct ttcagctgga gaggggatag ctgtagagtc
    25081 accttctttt ttttaccgat tacctctttt caaagcttca ggaacgcgtt tatttgggat
    25141 accgatggat gaagaaggga tcgacttaga aaaactgaaa gaatcgatcc ataaaaataa
    25201 attgaaagca gtgcttatca acccgaattt tcaaaaccct acaggaaaag tgatgagtca
    25261 aaaacgacga gaaaagctag tagaattgtg tcgacagtat cggcttccaa ttattgaaga
    25321 cgatgtattt acggatcttt catttctaaa ccaagaagaa gtccttccta tacgaacgat
    25381 tgatccggat aatgtgttat acgtcggttc gttatctagg gttttaggaa aaacaacgaa
    25441 aatcggttgg gtgataggac ctgagacttt tatctcgcaa ctagctcaag cgcaagaaat
    25501 gatggagttt tcgatgaata tccttacaca aatcacagtg gctaatgtat ttaatgaagc
    25561 aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa gaaaaaagcc gactgataag
    25621 cgcttgggga gattcacaat cgtttttcaa gcttcattca attttaggtg gttactatgc
    25681 ctggattact tgggatggga aaaaactaga ccccgaacta gcagaaaaag tagtagatat
    25741 gggtcttgga attgcaccaa gtctactttt tgggcaagat ataaatggaa tcagaatcaa
    25801 ttacagtagg ctggatgaaa accaaatacc gttgttttat tcaaagatga aaattctaga
    25861 aaagtggtta acagaataat ttcacaaagt aaatagatta aagaatataa cagcttggat
    25921 ttaaatttca agctgtttct tttttttcgg aaaaagaata cgatataaag aaactaagaa
    25981 aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa aataaatgtt tgcaacaaaa
    26041 ctgtataaca ccaacgtttt taaatagaaa cagatggttg taagtttgat tagaaaatag
    26101 aaaaaatgca gatgaaatat aaaacgcttt ccttattgtg attttattca ctaatgctgt
    26161 taatatgcgg attggcataa tttcctttca tcgtgaaaat ttgtgctttt agcttgtatt
    26221 tctcataaat ccatgataat ttattaatga agacggttaa atagtgatac agttttattc
    26281 actgtttcaa tcgcagaatg aagagatttt cttatagatg agagaggaat gtgtgacaaa
    26341 tggcaaacaa aaaagcaccg atcgacttcc aagcactgct agatgaagtc aatgcagatt
    26401 tcccagtata tcaagcactt gatcaagatg gaaacgtagt taacaaagat ttagtacctg
    26461 atttatccga tgatgaactc gttgaattaa tgactcatat ggtatggtct cgtgtattag
    26521 accaacgttc aacagcgctg aaccgccaag gacgcctagg cttttttgca ccaactgctg
    26581 gacaagaggc aagtcagctg gctagtgctt acgcatttga taaagaagat gttcttcttc
    26641 caggttatcg ggatgtacct caactagtaa aacatggttt acctttatct caagcttttt
    26701 tatggtctcg aggacatgct gcaggtaatt cttatccgga agaattgaaa gctctaccac
    26761 cacaaattat catcggtgca caatatgtac aagcagccgg tgttgcatta ggtttgaaga
    26821 aacgaaacaa gaaaaatgtc gtcttcactt atactggtga tggcggttca tcacaaggtg
    26881 atttttacga agcaatcaat ttcgctggcg cttaccatgc aaatgccgta ttttatatcc
    26941 aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca aactgcagca aaaacacttg
    27001 cacaaaaagc tgtcgcagct gggattcctg gtattcaagt cgatggaatg gatccgcttg
    27061 cagtatatac tgtatctaaa atggcacggg aatgggcgat cagtggaaat ggaccagtcc
    27121 taatcgaaac actgacttat cgttatggac cacatacact atctggtgac gatccaaccc
    27181 gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa agatccattg atccgcttcc
    27241 gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa agaagaacaa gtcatcgaag
    27301 caactaaaga agaaatcaaa gcagctattg ctgaagccga caaagtacca aaacaaaagg
    27361 tttcagattt cttgaaaaac atgttcgaag tatctccaca atcaatcaaa gaacaaattg
    27421 caatttatga agcgaaggag tcgaaataat catggcacaa aaaacaatga tccaagcaat
    27481 cacagatgcc ttagcacttg aactagaaaa tgacgagaat gtcgttgttt ttggtgaaga
    27541 tgttgggaaa aacggaggag ttttccgggc aactgaagga ttgcaggaaa agttcggaga
    27601 agaccgagta ttcgatactc ctttagctga atctggaatc gcaggattga gtttcggtct
    27661 tgcgttagaa ggatttcgtc cagttcctga aatccaattc tttggtttta ttttcgaagc
    27721 aatggacgaa gtagtagctc aaatggctcg tacaagatat cgaatgagcg gcactagaaa
    27781 tctacccatc acaattcgtt cgccatttgg tggcggtgtt catacaccag aattgcattc
    27841 ggataattta gaaggtttga tcgctcagtc tcctggtatt cgagtagtga ttccttccaa
    27901 cccttatgat gcaaaaggac tattgatttc ttctattaga agtaacgatc cggttgtttt
    27961 cctagaacac atgaaactat atcgctcatt ccgagaagaa gtgccggatg aagcttacga
    28021 agttcctttg gataaagcag cagttactcg tgagggaaca gatgtatcta ttatcacata
    28081 cggagcaatg gtgcgtgaag caatcaaagc agcagacaat cttgcaaaag aaaatatatc
    28141 tgttgaaatc attgatcttc gtacagttgc gccattagat gtagaaacga tcattcaatc
    28201 cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca caacgacaag cgggcgttgc
    28261 cgcgcaagta gtttctgaga tctcagaacg cgctatcctt tcattagaag ccccaatcgg
    28321 acgagtttct gcacctgata cagtcttccc atttggacaa gcagaaaatg tctggttgcc
    28381 aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg gaattttaag aaaagaaggg
    28441 acgaaacaaa gtcatggctt atcaatttaa attgccggat atcggtgaag gaatcgcaga
    28501 aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg atcaatgaag atgatacatt
    28561 actagaagtt caaaatgaca aatcagtaga agaaattccg tcacctgtca cagggacagt
    28621 taaaaacgtg attgttccag aaggcactgt agcaaatgtt ggggatgtat tagtagaaat
    28681 cgatgctcct ggacacaata gtgcaccgtc aactagtgcg ccatcagctg aagcaccaaa
    28741 agaaaaagtg gaaacatcag gttcagcaag tgtcgttgaa gctgccgatc caaataaacg
    28801 tgtattggct atgccatctg ttcgtcaatt tgcccgtgaa aaagatgtag atatctcaca
    28861 agtaactgca actggaaaag gtggcagagt aacaaaagag gacatcgaaa acttcttggc
    28921 aggtggtcca agcagtgcac cagcgaaatc agaagcgcca gaagcggcag caccgaaaga
    28981 agcagctcct gcagcagaaa gcaaaccagc tgcaccagca aaaccattca agtccaactt
    29041 aggagacttg gaagaacgtg tagcaatgac gccaacacgt aaagctatcg ctaaagcaat
    29101 ggtcaacagt aagcatacag ctccacacgt gacattgcat gatgaagtag aagtgtctaa
    29161 actatgggat aatcggaaac gatttaaaga agttgctgct gcaaatggta cgaaacttac
    29221 tttcttgcca tatgtcgtga aagctttaac agcgactgtt aagaaatacc cagtattgaa
    29281 tgcatctatc gacgatgcaa accaagaaat cgtttataaa cattactaca atatcggtat
    29341 tgcaactgat acggatcacg gactatatgt accaaatgtg aaagatgcag accgcaaagg
    29401 aatgtttgct attgcagacg aaatcaatga gaaagcaaaa ttagctcatg acggcaaact
    29461 atctgcagaa gatatgcgca atggaacaat tacgatcagc aatatcggat ctgtaggtgg
    29521 cggttggttt acaccagtca tcaactatcc agaagtagct attttaggtg taggaacgat
    29581 tgcacaacaa ccaatcgtaa atgctgaagg cgaaatcgtt gtcggtcgtg tgatgaaact
    29641 gtcattaagt ttcgatcacc gtatcgtaga tggagcaact gcgcaacaag caatgaataa
    29701 tatcaaacgt cttttagctg atccagagct attaatgatg gaaggatgat aaacaaatgg
    29761 tagttggaga ttttgctgta gaattagata cagttgtaat tggtgcggga cctggtggct
    29821 atgtagctgc catccgcgct gctgaaatgg gacaaaaagt agcaatcatt gaaagagagt
    29881 atattggcgg tgtttgtttg aatgtcggct gtattccttc aaaagcactg atagctgcag
    29941 gacaccatta tcaagagtca ctagattctg aaatgtttgg agtaactagt gaaaacgtca
    30001 aactagattt tgcaaaaaca caagaatgga aagaaaacaa agttgtccat acgttgactt
    30061 ctggtgttgg tttcctatta aaaaaacata aggttgaaac aatcgaagga gaagcgttct
    30121 tcgtagatga tcacacacta cgcgtcattc atccagattc agctcaaact tattcgttca
    30181 ataacgcaat catcgcaaca ggttcccgtc caatcgagat tccaggattt aaattcggag
    30241 gacgtgtgtt agattctact ggcggattag cattgaaaga agtacctaag aaatttgtga
    30301 tcatcggtgg cggtgtgatt ggtgccgaat tgggcggagc gtacgcaaac ttaggagcag
    30361 aagtaacgat tcttgaaggc tcaccacaaa ttttaccaac ttacgaaaaa gatctagtaa
    30421 aattagtaga agatgatttc aagaaaaaag gcgtaacagt cgtaacgaat gcaatggcga
    30481 aagaagcagt cgataacggt gacagcgtga cagtgaaata tgcagtggac gggaaagaag
    30541 aatccgttac tgctgattat gtgatggtca ctgtaggtcg tcgtccgaat acagatgata
    30601 tgggcttaga acaagcgggt gtcgaagtag gagaacgagg attgatcacg gtggataaac
    30661 aaggacgtac gaacgtacct aacatctatg ccatcggtga tatcgttcca ggggcggcat
    30721 tagcacacaa agccagttat gaagcaaaaa tcgctgctga agcaatctct ggcaaaaaag
    30781 tagctgtaga ttataaagca atgccggcag tagccttcac tgatcctgaa ttggcttctg
    30841 taggtatgac gatcaaagaa gcaaaagatg ctggtatcga agcaaaagca tataaattcc
    30901 cattttcagg gaacggtcgg gcattatcgc ttggtaaaac agaaggattc atccgtctag
    30961 ttacgacgat cgaagataac gtgcttattg gcgcgcaaat cggtggagtc ggcgcaagtg
    31021 atatggtctc tgaattagct ttagcaatcg aatcaggaat gaatgctgaa gatatcgcct
    31081 tgacgattca tcctcatcca tctcttggcg aaatcgtcat ggatgcttcg gaattagcat
    31141 taggtttacc aatccatatt tgatttgaat aatacaggcg aaacgcaact gaaaaatcag
    31201 ttgcgttttg ttttttagaa ttatcaataa tattattatg taaacttaaa aatatgacaa
    31261 cttcttggtt tattaccact tcctatgcta cactgaagag gaatatgaaa taggagagtt
    31321 gaagatgcaa gaatatcagt atccacttga tttagattgg acaacagacg aaatggtcat
    31381 tgtaatgaag atgtgggaag atctggaacg tgcgaatgaa aaagggataa acaatgaagc
    31441 ttttttgaag acttatcagc agtttaaaac tgttgtcaaa tccattggtg aggaaaaacg
    31501 attgggacgg gaatttgaaa aagcttccgg ttattcttta tatcgtacag tgcagcaagc
    31561 aaaaaaaaat ccaaacaaaa ctttgagaat gaacgggtga gataaatgga acagatgatt
    31621 gatcatatca agtcctggct tgaagaagct ggtgagaaaa tcaaatcttc tcaacaaaca
    31681 ttaacaatct cgcaaaaatc aaatcgaacg gatctagtaa caaatgtcga tcgaaacatc
    31741 cagaattttc taatcgaaaa aatccattcc tttgatccag aggcgaaaat attggcagaa
    31801 gaagaagggt gtaatcaatt atcggatctc gatggaagag tctttatcat cgatcctatt
    31861 gatggcacct tgaattttgt gattgaaggg gaaaattttt gtattatgct cgctgtttat
    31921 gaatccggcg ttggaaagct tggtttcatt tatgacgtga tgagaaatga acttttttgg
    31981 ggtggaaaag gaataggcgt gtataaaaat gatgagccat tgcctagacc agcaaataaa
    32041 tccttatcgg aaggcttact tggtgtgaat agttatcttt tcggtcataa taaatatgga
    32101 atccgagaaa ttgcggaaag cagcatgggg acacgtatgt acggatgcgc tggtcttgaa
    32161 ctagtggcaa tcttgaaagg aaaccacatt gcttatattt caaaccttag cccttgggat
    32221 tatgcagcgg gaaatgtctt attggaagag tttggtatgg taacaagcgg tctttttggt
    32281 actcctttga catttgaaaa aagggaatac tatttagcag ggacttcttg tgcatatgca
    32341 gaaatcgcag accttttgaa aaaagaaggc tgaaaaaaag gtagaaatca ttcacaaaaa
    32401 tgataaaaac gagcaaaaga ttaattttct cgaaaaaatg aaaacatttt actatatttc
    32461 atgaaaaaag tctgctataa ttagcagtcg agtaaaattt gccctgtcat aaagaaccaa
    32521 taaaaaagga gttagttaca ttgaattata gaaacgatat ccgtaacgtc gcaattattg
    32581 cccacgttga ccacggaaaa acaacattag ttgatgaact attaaaacaa tcagatacat
    32641 tagacgcaca tacgcaattg caagaacgtg cgatggactc aaatgcgcta gaaaaagaac
    32701 gtggtatcac gattttagcc aaaaacacag ctgtagacta taaaggtatt cgcgtaaaca
    32761 tcatggatac tccaggacac gcggacttcg gtggcgaagt agaacggatc atgaaaatgg
    32821 ttgacggcgt tgtgttggtc gtagatgcgt atgaaggaac aatgcctcaa acacgttttg
    32881 tattgaaaaa agcactggaa caacatatca caccaatcgt tgtcgtaaac aagattgaca
    32941 aaccttctgc tcgtcctgag catgtcgtag atgaagttct tgaactattc atcgaattag
    33001 gagcagatga tgatcagtta gactttcctg tgatttatgc atctgcacta aatggtacgt
    33061 caagcttgtc agatgatcca gcagatcaag aaccaaccat ggcacctatt tttgatacaa
    33121 ttattgaaaa aatccctgct cctgtcgata acagcgacga accattacaa ttccaagttt
    33181 cattacttga ctacaacgat tatgtaggtc gtatcggaat cggacgtgta tttagaggaa
    33241 caatcaaagt aggagaccaa gtagcattaa ttaaattaga cggaacagtt aagaaattcc
    33301 gtgtaacgaa attatttggt ttctttggac taaaacgtct agagattcaa gaagcaaaag
    33361 ctggagactt gattgctgtc tcaggtatgg aagatatctt tgtcggtgaa acagttactc
    33421 ctgtggatca tcaagatgcg ttacctatcc ttcacattga cgaaccaaca ttgcaaatga
    33481 ctttcttagt taataattca ccatttgccg gtcgagaagg taaattcgta accgctcgta
    33541 agattgaaga acgtttgatg gcagaacttc aaacagatgt atccttacga gttgaaccaa
    33601 caaactctcc agatgcatgg acagtttctg gtcgtggaga attgcatttg tctatcttga
    33661 ttgaaaacat gcgccgtgag ggatacgaat tgcaagtatc tcgtccagaa gttattgaaa
    33721 aagaaatcga cggtgtaaaa tgcgaaccgt ttgaacgcgt gcaaatcgat acaccagaag
    33781 aatatatggg tagtgttatc gagtccttga gcttacgtaa aggtgaaatg caagatatgg
    33841 tccatacagg aaacggacaa atccgtttga cattcttaac acctgcccgc ggtttgattg
    33901 gttattcaac tgaattcctt tcaatgacac gtggttacgg aatcatgaac catacttttg
    33961 accaatatct gccaatgctt cctggtcaaa ttggcggacg tcaccaaggg gcgcttgttt
    34021 cgatcgatac aggaaaagca acgacttatt ctatcatgag tatcgaagaa cgcggtacag
    34081 tcttcgtaga accaggtaca gaagtatatg aaggaatgat tatcggggaa aacagccgtg
    34141 ataatgattt gactgtcaac atcacgaaag caaaacaaat gacaaacgta cggtctgcta
    34201 ctaaagacca aacatctgtc atcaaaaaac caaaacaatt gacgttggaa gaatctttgg
    34261 aattcttgaa tgatgatgaa tattgcgaag taacaccaga aagcattcgc ttgagaaaac
    34321 aaattttaga gaaaaatgct cgtgaaaaag catctaaaaa gaaaaaataa tctgtaaaag
    34381 gatgaagaga gaaaccagct tgttataaaa aatagctggt ttcttttttt gtccaactct
    34441 tttctgtgga gaagtcatct ttgtcaatgg acataaattc agaaaatacg ccgaaagacg
    34501 tttaaaagac ttttaaaaaa cttatttcta gagagttttt gtttcataat aagataatgt
    34561 ttcacgaaag gagatcaact tgtccttcga agaaaatcga agtaaaatgg caagagaaag
    34621 gagcgggaag atgaacgact tattaaaaca atactttggc tatgatgagt ttagacctgg
    34681 tcaaaaagaa attatccaga aagtcattga tcaggaaaat gtattaggaa tcatgcctac
    34741 cggaagtggg aaatcaatct gttaccagct tccagcttta ctattagatg ggcttactgt
    34801 ggttgtgtcc cctttaatct ctttgatgaa ggatcaggtg gacgcggcga atcaattagg
    34861 tatacccgcc acttttatca atagctcgtt agatggttac gaaacagcta gacgcttcca
    34921 agaaatcgat agacagcagt accgtttact ttatatcgct cctgaacgtt ttatcatgcc
    34981 ggattttatc caagcaatga agcgatggaa cgtccgtatg atcgctattg acgaggcaca
    35041 ttgtatctct cagtgggggc atgatttccg cccaagttat ttacagatgg cgaaccagct
    35101 agatcaactg ccaaatcgtc ctgtaattgt tgctttgaca gctacagcga ctgtgcaagt
    35161 agctgctgac ataaaaagac ttttaaaaat tccagaaaac aaccatattc agactggttt
    35221 tgaacgagaa aatctacgat ttcaagtaat taaagatcaa aaaaaagaac aatatctaat
    35281 cgaatattta aagatcaaca aaaatcaatc tggtatcatt tatgcagcaa cgagaaaaga
    35341 agtagaccgt ctctatcatt tgcttagaaa attcgatttt tccgtgggta gatatcatgg
    35401 tggtctaaat gagaatgagc gaacagaaat gcaagaagcc tttttatacg atcgcttgca
    35461 gctgattgta gcgacaaatg catttggtat gggtataaat aaaagcaatg tccgttttgt
    35521 tatccattat caaattcctg gctctctaga agcatactat caggaagctg gtcgggcggg
    35581 aagagatggt ctttccagtg aagccatcct gcttttctct cctcaagata tccaagtcca
    35641 aaagtttttt atccagcagt cccaaagaga agaagggcaa aagcaaaaag aatatgaaaa
    35701 attaaaagcg atgacagaat acgtgtatat tgagtcctgt cttcagcaat acatcctaaa
    35761 ctattttggc gagacaagca gtccgtgcaa ccgttgtggt aactgtttag atgacagaga
    35821 aatagtagaa gtaacgacag aagcacagat ggttctttct tgcttaaaac gtatgggtga
    35881 gaactatggt aaacaaatgc tgatgaaagt cttagcagga tcgaaagaac aaaagctgca
    35941 agctttaggt tttggacatt tatctaccta tggactgatg aaaaaccaat cgcaaaaaga
    36001 aacgatgcag ttaattgaat atttgatatc gaatggctat cttctgacca tcaatggtga
    36061 gtatcctgtt ttgaaagtaa cagaaagagg gatacaagta ttgaaaggac aagaatctgt
    36121 ttatcgaaag gaacccaaga aagtccagca gctatcggat gaagaaaccg atacactatt
    36181 tgaagtgctg cgggaactgc gaacggatct agcatcagaa gcaggcgttc ccccgtatgt
    36241 cgtattttca gattccacat tgaaagaaat gagccgaata cgtccatcaa gcagattgga
    36301 gatgcttcag ataaaaggag ttggccaaag caagcttgat aaatatggcg aagccttttt
    36361 atctcggata aaaaatgcgg actctaacgt tctggataag tagaaagaac gaatacgtaa
    36421 aaatgaataa aaacaactgg ttaaatctgt tttttttgct ccttatgaaa atcaaatatg
    36481 ttataattaa ggaattagaa tgggaggtaa gcacagatgg attctttgtc gaaaaaactt
    36541 gcgatccaag tattaaatga tgaagcagaa cggatcaaac gattaatccg aaatcaaaac
    36601 aacagtctct gtatctcaca atgcaaagcc ttcgaagaag tggtagatac acagatgtac
    36661 ggcttttccc ggcaagtcgg ttttgctgtc cgtataggag taatcggaaa gaacgagggc
    36721 cagctgatgt taagtgagct agaacgagaa ttgaatcagt tatatagtga ggtttaccaa
    36781 gaaatttatg ataaaaaaga agttggcaag gaggaataga cttgcctaag aaagtgaaaa
    36841 aaaggcatct attagactat agcatcttga ttccctattt aattttatgt gtattgggat
    36901 tgatcatggt atatagttct acgtcgtatc tattattaga aaacggacaa aatccgtccg
    36961 catcagtcat taaccagagt attttttggg tgcttagctt gattgctatc gcactacttt
    37021 ataaaatgaa aacagatgtt ttgaaaaacc agcgtttgat catggcagcc atcgcagttt
    37081 tgacgatttt gctgctgatt gttgtgtttt ttggtaagga gatcaatggg gcgaaaggtt
    37141 ggctgcaaat tgcaggattc tcgatccaac ctgccgaata cctaaaaatc atttctatct
    37201 ggtacctgtc tctgacttta tctaaaagac aaaacagtgt acaaaaagat tttcttggga
    37261 ctgtgaaacg tccacttgca atggttatcg gacttactgc attagtcgct attcttcccg
    37321 attttggtaa tgcggcagtt atctttttga ttattttggt tctgctttta gccagcggag
    37381 tcaattatgt gtatactttg attgtaggag taggaggctt ttgtctaagt acttttacga
    37441 tctggctgat caatatcaca aacggaaaga tcttacccgg acgtttacag tatatctata
    37501 atcgatttgc tatttaccaa aatcctttct cagacgaatt aaataaaggg catcaactag
    37561 tcaacggcta ctatgccatg ttcaacggag gactatttgg caggggatta ggtaacagta
    37621 tccagaaaaa aggattttta caagaagcac aaaccgattt tatttatgca atcgtagtgg
    37681 aagaactggg agtgattatg gggattttga tattagcgtt gctattcttt atgattgtgc
    37741 gcattgtcct tgtaggtatc cgttcaaaag atccgttcaa ttcgcttttg tgcattggga
    37801 tcggcgcaat gtttttgatc caagtatttg tcaatcttgg agggattact ggcgttattc
    37861 ctttgacagg gatcacgttc ccatttttaa gtcaaggagg ctccagtttg ctgatgcttt
    37921 cgatttgtgt cggctttgtc ctcaacatca gtgcggatga aaaacgcaag agcttaggtt
    37981 tataataaaa aaagatgtag gagtggttcg atgaaaaaag ttttagttgc taacaggggt
    38041 gaaatcgctg tacgtgtatt tcgtgcttgt acagaattag gaattaaaac tgttggaatc
    38101 tatgcagaag aagatgaata ttccgtacac cgtttcaaag cggatgaagc atatttagta
    38161 ggacaaggaa aaaaaccgat tgatgcgtat ctagatattg aagggatcat ttctatcgcg
    38221 aaagaatgcg gagctgacgc tattcatccg ggttacggtc ttttgtcgga aaacttgaat
    38281 tttgcgcaac ggtgcgaaga agaaggcatc atttttgttg gacccaaact gcatcattta
    38341 gatatttttg gagataaaat caaagcaaaa gctgcagcta tcgaagcggg gattgcttcg
    38401 attccaggca cagatggacc aatcgcttct atagacgatg ccttggagtt tgcgaaacaa
    38461 tacggttatc cgattatgat caaggcagct ctaggcggcg gcggacgcgg catgcgtgta
    38521 gctcacgatg aaaagagtgc aagagagggc tacgaacgtg cgaaaagcga agcaaaagca
    38581 gcctttggta gtgatgaagt gtatgttgaa aaatacatcg ctaatcctaa gcatatcgaa
    38641 gtacagattc tcggggatac gcatggaaac gtcatccatc tttttgaaag agattgttct
    38701 gttcagcgcc gtcaccaaaa agtagtagaa gtagcgcctt gtgtctctat gaatgaacag
    38761 caacgtcaaa aaatctgcca ggcagctgtc caattgatga aatatgtcgg gtatgtcaac
    38821 gcaggtacag tagaatttct tgttgaagga gatgattttt actttatcga agtcaaccct
    38881 cgcgtccaag tggaacatac gatcacagaa atgattacag atattgatat cgtaacaacg
    38941 caactgttga ttgctcaagg tttggatctg cataaagaaa tcggattgcc tcagcaagaa
    39001 ggaattaaat taaatggttc agccattcaa tgccgaatca ctacagaaga cccgttgaat
    39061 aactttttac cagacacagg gaaaatcgat acgtatcgtt cacctggtgg ttttggcgta
    39121 cgtctggatg tcggaaatgc ttacgcgggc tatgtagtga caccgtattt tgattcattg
    39181 ctagtaaaag tatgtacgca tggtgctacc tttgaaacag ccattcagaa aatggaacgt
    39241 tgcttgaaag aatttagaat ccgtggagtg aaaacaaaca ttccatttat gctcaatgtc
    39301 atcacacatc ctgaattcca atcaggaaat gcaaaaacaa ccttcattga tagtacagca
    39361 acattatttg aatttcctcg actacgagac cgcggaaaca aaacgatgaa atatatcggt
    39421 gaaattactg tgaatggttt tccaggtatc gaaagtggcg aaaaaccatt ttatgaagaa
    39481 ccacgtatgc ctaaagatct aatcacaaga actgattacg taacggctaa gaatgtatta
    39541 gatgcgaagg gtgccgatgc tttggtcgaa tggatcaaag gacaggaaaa tttgttgttg
    39601 acggatacga catttagaga cgcccatcaa agtctgctag caactcgtgt tcggacaaag
    39661 gatttcaagc agattgcacg tttgactgga gaaggtcttc ctgaactctt ttcaagtgag
    39721 atgtgggggg gagcaacatt tgatgtggct taccgctttt tgaatgaaga tccttggcaa
    39781 cggttgagaa aaattcgttc tttaatgcct aatacgttgc tgcaaatgct tttcagaggt
    39841 tcaaatgccg ttgggtattc taattatcct gacaatgtat tggtggaatt tgtcaaagaa
    39901 gcagcagcgc aaggaatcga tgtatttcga atctttgata gtttgaactg gactccacaa
    39961 atggaaaaaa gtatccaagc tgtacgggat actggtaaaa tcgcagaggc agctatttgc
    40021 tatactggcg atatcaacga tccaagtcgg gcaaaataca atgttcagta ttataaagac
    40081 atggcaaagg aactagaaca actgggtgcg catatcattg caatcaaaga tatggctggg
    40141 ctgcttaaac cgcaggcagc ttatcgtttg attagtgaat tgaaagaaac gacagatctg
    40201 ccaatccatc tgcatactca tgataccagc gggaatggaa tcatcactta ttctgctgct
    40261 tcaaaagcag gagtagacat cgttgacgtg gcaatgagtg caatgagtgg gaatactagt
    40321 cagcctagca tgagcagctt gtattatgca ctggtcaatg gtccacggct gccagaaatc
    40381 acgattgaaa atgcacagaa attaaaccat tattgggaag atgtgcgcat gtattacaaa
    40441 ccgtttgaaa acggcttgaa tgctcctgaa acagaagtat atatgcatga aatgccagga
    40501 ggacagtatt cgaatctaca gcaacaggca aaagctgtcg gattaggtca tcgctgggat
    40561 gaaatcaaac aaatgtatca tactgtcaac ttgatgtttg gcgatatcgt taaagtaact
    40621 ccatcatcta aagtagttgg agatatggca ctatttatgg tccaaaatga tcttacagaa
    40681 gaagatattt atgaaaaagg cgaaacacta agtttccctg aatctgttgt gactttcttc
    40741 caaggagaac tagggcaacc cgttggcgga tttcctaaaa aattgcaaaa gattattctt
    40801 aaaggcagac ctgcattgaa tgaacgtcca ggtcttttgg ctgaatcagt cgacttcaac
    40861 gaagtaaaaa aagaattggc agaaaaaatt ggctatgaac caaaacaaga cgaagtatta
    40921 agctatttga tgtatccgca agtctttttg gactatcaaa aagcatataa tcaatttggc
    40981 gatgttacat tactggacac accaacattt ttccaaggta tacgtttggg tgaaacaatc
    41041 aatgtacaaa tcgaacgcgg aaaaacgctt atcattcgtt tagatgaaat cggagaaccc
    41101 gatatcgaag ggaatcgcgt actgttcttc aatttgaatg gacaaagacg tgaaatcact
    41161 atcaaagata attcgatcat cagtacggtc caaacccgat tgaaagctga accgacaaac
    41221 cgagaacaaa ttggagcaac gatgtctggt tctgttttgg atgtattagt gaaaaaaggc
    41281 gataacgtta aaaaaggtga tacgttgatg attactgaag cgatgaaaat ggaaacggcc
    41341 atcgaagcac gttttgacgg agaagtcgca catgtatatg tgtcttcagg agacactatt
    41401 tcgtctggtg accttttgat tgaagtgact gagaaataat aagcgaaagg tggggaaaca
    41461 tgaaaagact gggtttgttt tggctgattc tgctattagt tattgtggca ttctacatgg
    41521 aaccagtctt gtttccgcca aaacttgaag tgaaggaaaa ctctgatcaa caaatatcaa
    41581 accaaatgac aacgtggaaa taccaggagc ttaaagcaac tggtttttct acttatatcg
    41641 gaaaaccgat cgatcagctg gaaaaggatt ttggccaagc ctatgatcga ttaactagcg
    41701 gatttggttt tgaaacaaga tattatgaaa ataaagctag tcatctcaca tttgaagcga
    41761 acatccaaga cagcagagtc acaactgtta aagtactgca aacagggcct gctgatatcg
    41821 cgccgtttta tttagggatg acgatgcaag atcttacgaa aatcactact atctatacta
    41881 atttcacttt tgagtaccat gatacagaag ttggaatcga actaatggaa gaggatatga
    41941 attatcgtcc gctaattgct tttgataacg acacttttgc tattttattt tttgatcaaa
    42001 caacaaacgg cctattttca gttgtgtatt tagataaaga tagtcttttg aaactattgc
    42061 catatcaagt gtttggtgaa ggccttccac attatcaagc agataaatcg gctgattggg
    42121 aatcaatcaa tcgcgttaaa gaacgaaaaa gtacgacttt attaaatatg cttagaagag
    42181 aagacgaact tcccatatac aatcaaacac ttgcctttca aaaccacagt cagctgctac
    42241 tacatgattt tttgacaagg cctgaggaaa tattgtcaga ggaacgtatg gctgattggc
    42301 aaaaatcgct aggcagtgct caaacgacag tgaaattctc actgacgaat gatgaattgg
    42361 agaagttaat cagtaaacaa aaattagaaa atactagtgg gctatttatt catccggcga
    42421 ttgacgctac tttttcgttt ttatattggt acagcgatcc ttacaaccat gaccgtttca
    42481 tggacccggg aacggattct gtaggcattg ctttttctaa agaaaatatg ttagtcttat
    42541 tgcaagaaga aaacggagaa agtagtgata gcagtgatcg ttaacgaaga gctgtttcaa
    42601 ctggaggatc aatgcctcca agtcgctgaa atgataaaaa agagtaaaac gatggcttct
    42661 tatcttgaac ataaacagtc aatggaagaa tctgcaaaag tttgccagtt gaaagaagcg
    42721 ttcttgtcta agaaagaagc atttgaaaat gtagcggcat atggtgagta tgcacctgat
    42781 tatcgtgaaa aaaaacgagc tgttcgaaaa gcaaaaagac agcttgatat ggatgagcat
    42841 gttgcagctt tccgatatag tgaaacacag ctgcaaagtc tgttggatca aattgtccaa
    42901 gtacttgccg aagaagtttc taatgaaatc aaagtagatg caggtagccc gttttttgaa
    42961 acgaaaaaac actttggatg cggaggtaat tgtcatgcaa ttagacaata gtgaatttga
    43021 aataacaaaa cgacgcgggc tgatcgtatg ggtctacagc ttaaaacaat taaagaatct
    43081 gaaaaagttc ggcttgttgc attatgtctc aagaagaatg aaatatgtcg tgctttactt
    43141 aaatgaagag acattggaag aaacagaaga acgaatccgt aaattacatt ttgttcgaaa
    43201 cgtagaacgt tcttaccgac cggatatcga aatgaatttt gccgaaaaaa taggaaacaa
    43261 aacagtatta aaagacgaag gtttcgaaat tgaagagcta aatacagaaa tccgattagc
    43321 tgaacatctt taaaaatctc ttatgagcag tttttaaata aaaaataact tggataacga
    43381 tcgatttttg atcattgtcc aagtattttt ttggtatact agtaccagta ccatttctaa
    43441 ggagtgagta gatgcggata atatcaggaa attatggcgg gcgccgttta aaaagtttag
    43501 caggggcgaa caccagacca actacagata aagtaaaaga atccatattc aatatgattg
    43561 gaccatattt tgatggagag acagtattgg atctgtttgc aggaagcggt ggtttagcta
    43621 tcgaagcagt atctagaggc tgcagtcatg cggtatgcgt agacaaaaat taccaagcgc
    43681 taaaaatcat caaagaaaac atcgaaatta cgaaagaacc agagaaattt accacaataa
    43741 aacttgatgc ggacaaagca atcgaacggc tagcagcgga gaaacaacaa ttcgactact
    43801 tgtttcttga tccgccatat gcaaaacaaa aaataatcga tcaaatccat aaaatggaag
    43861 aactgggact tttttctaaa accgcagtta tcgtatgcga aacggataaa acagtcgaat
    43921 tgccagaaac tgtggctgga ttccatcaaa taagaaaaca gatttatggc ataacggccg
    43981 taactatcta tagaaaagag ggaaatcaat gaaacgtgca ttattcccag gaagttttga
    44041 tccttttaca aaaggtcact tagatacggt ggaacgtgcg gctaaattat tcgatgaggt
    44101 agtaattggt gtttttatca atacttcaaa gaaaagtttg tttcccccag aagaaagaat
    44161 gacattgatc acaaaagctg tttctcatct tcctaatgta aaagtcatgc atcaagaaaa
    44221 tcaattgacg gtagaaacag caaaagagat tggtgcagat gcgttgatca gaggcatacg
    44281 aagtatcaaa gactttgaat atgaacgaga gattgctcag atgaatcatc atctatatga
    44341 tgaattggaa actgtcttct tattagcaaa accagaatat agtcacgtga gttccagtat
    44401 tttaaaagaa gtactgcact ttggcggaga tgtgagcagc tacttaccgc cagtgatcaa
    44461 cgaagcttta gctggaaagc gtgaatcaaa tgaaagctaa acaatggttg aaaacagtac
    44521 tgattatcat atttggaatg ttgatcattg gatttgtagt agttcccttg ccgtattatg
    44581 tagaatcacc gggagccacg atcgatctga aagaactaat tacggtcaat gataaggaag
    44641 ataaaggttc tgggtctttc tctcttacct ctgttggtat caggcaagct acaggtttct
    44701 tagctttaaa agctaaaatg accccttttg aagaattgat cagtgaagaa gaattgactg
    44761 gtggggcgac aaatgaagaa tataatcaaa tgcaacagta ttatatggat tcttcccaaa
    44821 atgcagcaat cgaacaagcg ttaaaacttg cagacaagcc ttataaaatg gaattcaaag
    44881 gcgtatatgt catgagtgta gagaaaacat cgaatttctt tggcaagctt tcagttggag
    44941 atacagtaac aaaagtagac gggaaaagct ttcaaagtac agaagcattt atggattatg
    45001 taaaaagcca aaaagttgga caaacaatcg aaatcagtta tttgcataat ggtgaagaaa
    45061 aaacagcttc tggtgatctg atcgagttac caacagataa aaaggcagga ataggtatca
    45121 cgcttactga ccataccgag atcgaatcag atacagatgt tcgtattaat tctggctcta
    45181 tcggtgggcc atcagcggga ttgatgttca ctttagaaat ctatgaacaa gtcactggta
    45241 aaaacttgcg acatggtaag caaattgcag gaactggaac gatcaacagt gaaggagaag
    45301 ttggccgaat cggaggaatc gataaaaaag tggcaagtgc ggataaagct ggcgtagaga
    45361 ttttctttgc accagatgat acgatttcag acgatgttaa gaaagaatat cctgaaataa
    45421 aaaccaacta cgaagaagca aaagctgccg ctaaaaaatt gaattcctca atgaaaatcg
    45481 taccagtaaa aacagtacaa gaagcgcttg actatctaga gaaaatgaaa tgaaactaaa
    45541 taattaaaga ggggttgtga cacgagttct gtcacaaccc ctctttttcg tcaaaaatct
    45601 tcacgataaa aataaaaaca aaagaaaaaa ttgacgtaat tagatgttga agggacgatt
    45661 ttgccagagc tcactgtttc ttcaatttta aatgaaagga gtaatgaatg atttttactg
    45721 gtcattcttt gaaaaaagaa caatgataga aaaaatcagc aagaaaatac tagcttcacc
    45781 caaattgatc atttggatgt ctatgatagt tcttatcttt tgtatcgcaa gtggtttttt
    45841 attatcaaat ttgctatttt atagagaaac agaagagact gctgcaaaag aaatactttc
    45901 ttcgtttagt tccagtggag aagatccatc agctgaagga aacgatgtag aagaggatgc
    45961 tcgtccaaag gtcatgtaca ccgatatcaa aggaagtgtg aaggagcctg gtatctattc
    46021 cttttcttca gaagaacggg tctatgatgt actaaagcgt gcaggtggat tattagaaga
    46081 agcggatagc gatcgaatca acttttctgc gaagatagaa gatcagcaag tcctatatat
    46141 ccctgctgta ggtgaagagc tgccagaaca tcttgatcag ttggcatcct cggaaggaaa
    46201 acagcctaca gcagattccg agcctcccaa aataaatatc aatacagcca ctccatcaga
    46261 acttcagcag attcctggga tcggatcagt caaagcgcaa gaaatcattc gatttagaga
    46321 agaaaacgga tccttccaga aagtggaaga cctgcaggag atttctggta tcggtgaaaa
    46381 gacagttgaa aaactaaaaa acttcgttac aattaaatag aatgattcaa aatcgactgg
    46441 cacgagcaat aaatggacgc taatggatac taagaataat ccaattctaa gagcttggca
    46501 ggacgaggag gtagtatttt tggataggaa atagcttgat tgttcgcaaa gaaacattaa
    46561 aaaaaccaat gctgaatgtc tatctccaaa ataaaatatc tgggattcat attatgaata
    46621 cggctgtcag cggcaataac agtcaagctc tacgagaacg ctttgccaaa gatgtactta
    46681 gctatacagc agataaagta tttattctca ttgggacgaa tgatctagca gagcataaac
    46741 aactttctaa agaaacgtac caaaaaatct gcagtggatg atagttcagc tgaaaaaaga
    46801 ctatagttct gatcatgctt tctttatcac gccaccagca gtcgatgaga caaaacaaaa
    46861 atatcggaat aacaagttga tcaaagaata tatctctgtt ctcacagaag tagcaaaacg
    46921 ccatgattgt gaagtatcga tttttatacg gaatttcttg aacaaatgga ttcgaagaat
    46981 ttttataatg ggctaatgga tgacggactt catttcggca gtgaagggta ggatcttttg
    47041 tcagacagga tgattcgtta tatttaaaga atattttgtt taagcaatga gtacttttta
    47101 atcaacttga aaaaacggag gaatataaaa catgttagaa aaatcttcaa aagcatgcga
    47161 tacgaataaa gaaaaagtta ctgataagtc acataatgaa cgcattcctt gggaccagta
    47221 ctttatggct caggcagtac tgttatcttt acgtagtacg tgtacacgtt tagaagtagg
    47281 agcgacactc gttcgagaaa aaaggatcat tgctggcggc tataatggag cagtcagtgg
    47341 agatgtccac tgcatagatg aagggtgtta cgtcgtggat ggacactgct tgcgaacgat
    47401 ccatgcagag atgaatgcac tcctacagtg tgcaaaactt ggtatcccga cggaaggttc
    47461 agaaatctat gtcacacatt ttccatgttt ggcctgtaca aaggctttgt tgcaagctgg
    47521 tgtgaaaggg atcaattatc tccatgacta tcgaaatgat ccttatgccc aagcgttgat
    47581 cgaacaatta ggtgtatctg tacaccaagt tgctttagac tcacagcatt tttcaaaact
    47641 tcaaaatgaa ttaacaggaa aacatgtcta gcgttttaga aaggcatgtt cgaaatcggt
    47701 tgatttttcc ggcaattacg tcgatgatta tctgtttgtt gtattacact gacaattggg
    47761 gttatcgttt gcttcttgcc ggaatttttt tatggttttc gctaaaagaa ggacgtttta
    47821 tcgcgtactt gtgtattttg tgtagcataa cggtatccat ttcttatagg ctacaagagt
    47881 attcattttt agaggaaaaa acaacatctg cgcaaattac tgtatttaca gatacgattc
    47941 gtgcaaatgg taattttttg acaatggaag gaaggttatc agagaataaa caaaagctcc
    48001 agctttctta tcaagtaaac acagaagcag aattgaaaac atggcttaac ttccgaggaa
    48061 agggaataga attgactgct gaaggcgagt tcatctctcc agcaaaaaag cggaatcttt
    48121 ataattttga ccaagaagct tacttcagga accatagaat ttctggacgg ttccaaattc
    48181 aaaagattga acagatcacc atgcaaaatc actggcgaaa ctggcttcag agaaaaagag
    48241 gatcttttat ctcctttgtc caatctgaat ttccgcaaaa aacaagtgta tatattaatt
    48301 ctttattatg gggctataaa gatgcaggat tcagagaacc agaagaaatt tttagggaga
    48361 gtggtttgct gcatctgttc agtctatcgg gtctacacat ccaattttat ttagggtggc
    48421 tgtattattt atttcgacgt atcggatggc cactacacgt cagtattatt ccgttgagtc
    48481 tactaactat ctgttatgtt tctattgcag gaagttctat ttctgttttg cgtgctagtt
    48541 tgctgttttt attgcgattg ctagtcaaat tgctgaataa gcattattcc accatggact
    48601 tatttgcagt tgtcttttgg cttcttttac tatttgatcc tcgatttctt tttcatgcgg
    48661 gtggacagct tagttttttc atgagttttt tgtttatcct gatttctttt gatggagatt
    48721 ggttgaaaaa aacggcctct cacgtcctgt ttactatgat cacgatgccg ttgattgtat
    48781 ggcattttta tgagtggcct attttaggta gtctttttac attactgatt gcaccggtat
    48841 tcaagtttct ttttcttcct ggtgtgttat ttctcacact cttcaatcgc tggctgccgg
    48901 aaactttact tttgctgata gaagagggat taatcttatt tgagcgaata atcgattttt
    48961 cttcagggtc gtctctcatt ataggacgtt tgccccttgt gctatctggg atgtatattt
    49021 tcggattact gatttgtatg gataggttga aagaacaacc tgtgaaacac ttgttttatg
    49081 ttggtctttt ttcttttatt tttttgactt tgccattttt acgcttttct tcaacgatcg
    49141 cttttatcga tgtcggtcaa ggagacagta tctttttgca aagtcctttt cgaaaagaaa
    49201 cgattttgat cgatactgga ggtcagttac actttgaaag agaaaagtgg gcgcaaggta
    49261 tgatacgtcc tcctgctgaa agaaatatcg ttccatatct aaaaggtcag gggataagtg
    49321 taatcgataa actcatcttg acacacgacg atacggatca tgtgggagaa ttacccacgt
    49381 tatctcagca ttttactatc aaaaaaattt atatcggttg gggcgctgga cgaaatgagc
    49441 cattacaaag gcatttatct gaagctcaaa aaaatggtac tgagatcatt gaaatcaaac
    49501 atggggacag gatcagcggt tattatgatc tttatgtatt gactcctttt gaaaagggag
    49561 agggaagaaa tgaagattcg attggtctat ggatggaata taacaatagg cgctttttat
    49621 ttttaggaga tttgaatcaa gcaatggaaa aacaattgct tttgatttat cctaatctga
    49681 aagccgatgt agtcaaatta ggtcatcacg gtagccgtac gtcaagtaac acagattttc
    49741 tttcccatat tgaagcaaaa catgggatta tatcctgcgg cgtaaataat cgttatggtc
    49801 acccgcacag cgaagtattg gagacactag aagaaaatca gataaaaaca tggcgaacgg
    49861 atcaacaagg aatgatcacc tatcgctggc atccagtttt tcatcctcat gggctagtgg
    49921 aaacaatgat agactagtaa ttctcatctg ctcatggtag gataattttt gaaagtggtg
    49981 agtaatttga gtatacaagc agagttacaa aaaatacgta aagaaaaact tgcatcttgt
    50041 tacttgattt taggaacaga aaagtttctc caagatcaag ttcggacaga aattgtcaaa
    50101 aaaatccaat tagagggcga agatgactta aattttttaa gttttgacat ggaaaatgca
    50161 agtttagatg aggtagtagc tgaagcagag acgttgccgt ttttcggaga tcaacggtta
    50221 gtattcgtag agaatcctta ttttttaaca ggtgaaaaag taaacaatgg aatcgaacaa
    50281 aatactgatt tattgacggc ttatttgaaa gaaccattag aatcgactgt attagtattc
    50341 tttgcgccat atgaaaagtt ggatgaacgg aaaaaagtaa cgaaacaatt gaaaaaaaca
    50401 gcaattatca tcaatgtcca acagttgaat gaaaaagaag tacgtcaata tctgatgaat
    50461 accttagaga attcctcaat caaaatggaa cgaagtgcaa ttgatttatt cttgaggttg
    50521 accgacttag atttatccaa aatgatgaga gagttacaga aattggtcct ttatggacaa
    50581 aatcaacagc aaatcacggt aagagaagta gaagaattag ttcctaaaac gttagaacat
    50641 aatatttttg acatgaccca atatatttta aaagggaaga cggaacaagc tttgcgttta
    50701 tacgaagatt tagttatgca aggggaagaa acaatcaaaa ttaatgcgat tttgctttct
    50761 caattacgtt tatttttaca gacaaaattt cttgtaaaga ttggttatca gcaggcaaat
    50821 attgctgaaa cattgaaaat ccatccatat cgtgtaaaac ttgccatgca ggaagtaaaa
    50881 aaattcgatg aacatttgct ggtacagttg tttgatcaat tagttgagat ggattatcaa
    50941 atcaaaacgg gacaaatcga aaaagaatta agttttcaac tattcgtttt gcgcacaggg
    51001 cagttgttaa gaaatcgata agaagaaaga cgagggcaaa gcatgcggga aggtcgtttg
    51061 tttttgtcct ttttttgaca aataaaaaaa tcggccgaag ccgattcttt tatttagcga
    51121 tttttttgct taaacgagat ttgtcgcggt tagctttgtt tttgtggatt agaccttttg
    51181 attcagccat atcaatagct ttaacagctt ctttatataa cgcatctacg ttgtcagcac
    51241 ctgaagctac agcatcttca aattttttga ttgcagtacg catagcgctt gtttgagatg
    51301 aatttttcac attagcgttt tcgcttgtac gaacacgttt gattgcagat tcaatgtttg
    51361 gcattagttt cacctccgat aacttaagtt cttacgcgtg ttaccgcata attcaacatt
    51421 cattattata cctaaaggca gcgtgctttg caatatgaac atgacaagtt tttttcctta
    51481 acagtctttt attaggattt tttttcagta aaaacggata gtacaagctg aaaaaaatca
    51541 aaaagacgct attgtgggag gcaatagcgt caaaccaaac tagattttta tttatctatt
    51601 taaacaaagt ttgaatggga atgtttggta ctaagttatt atataacaaa gagaaccgtt
    51661 tgcaaagttt tttttgaaaa gggatacgat tccctgttgt tctttgtttt tctattaaga
    51721 aactgcagtt attttataac cagtatcctt tgatcagtaa aggctattct ggtgtaccga
    51781 ttattttaat gtattcatcg atatttttta tcaacatttt ttttatgacc aactggttcc
    51841 gacggttcca tttgccactt acttccagca catatttatt taggggcaaa cgtaagatat
    51901 attttgccaa atagcgatct ttaagcaggc cattgatcac acggtcctca agctgcaagt
    51961 tgatcccagt cagcatcatt ggctgttctt gtaaaacgta cgtatcaatt aaaagacctt
    52021 caaaagtttc cacaagcgtc ttcctttctt ttcaaagtaa cctgaaaaca ggcactaatt
    52081 cttccatttt gatattcgtt gaagtggatg cgatcattct ttcttttgtc cactgtaatc
    52141 tagtataaca aaaaagagca gaagagccaa aaagaaaaaa gagcatcgaa ttctatagga
    52201 agtctcattg gcacggccta aattattaca aaacattgat taaaaaaagc ccctgcatcg
    52261 caggagcatc ccctatatcg ttttaatagg tcaatcaaaa agcagtatca aacacatttt
    52321 gtatcaaatc aggaaataat agataagcta agcccagaat caaaagtacc gctagaagaa
    52381 accagccgat tgttcctatg aaatacttta atttttcgaa cattgtatca cctcgttgtc
    52441 agtatatcac ggatcagtaa attgggggtg ttaagaatct cctaaaatag acaaaatttg
    52501 ttctattttc ctaaagcaaa tacttgtaag atacagtaag tgataccaga agcaatgacc
    52561 ggtccggcgg cgattcctct taagaagaca acacctaaaa tcgtgccgaa aacaagtgca
    52621 acagtgattt gtggatcaga agcaatcaat cctacaccct tggaagataa gacagccact
    52681 agcccaccgc aaaaaacagc tacccagcca ataggggatt taaaggcttc taatagatca
    52741 cgtagaccga tttctcctgt tgcgataggg attagaatag ccgctgtgat gatcgtcacc
    52801 ccaatattga ttcctttgcc ttgaagccaa gggaaaaagg aacgggaggc tggtatgagc
    52861 ttcataatca aaacaaaagc tgccgctatc atcagacttt gatttttagc taaaattgct
    52921 acaagtaaaa taattcctaa aaaaagccaa ctttccatga aatatattcc tcactttctt
    52981 cgaaacaatc ataacatgtt tttagaaagt tacaaaagta ggaattcatc cttcaagacc
    53041 tattcataag ctgttttttt cataaaataa acaaaatcat aaaaaaacac tttgcttatt
    53101 tgacagaaga cagccgcgct ttgtatagtg gacaagactt acattttttt gaagaatcag
    53161 aaagatcagg taaaggtctt agtaaacgca agccattcag aataaccaat attgtgcttc
    53221 cttcgtgtcc tacaactcca agaggtagat ttaaaaattg caggaagtta gaagcaatca
    53281 acaagagaat aacaagaatc gaaaaaataa tattttgttt gatgatccgc cgcattttta
    53341 aagatagccg atgactgtag acaaatttat ttaagtcgct tttcattaag acgatgtctg
    53401 cgatatccat agcggcatct gtcccttgcc ccatagcgac acctaaagaa gcggctgcta
    53461 aagcaggcgc atcattgact ccatcaccaa ccatcgcatt tacttggtaa agttcattct
    53521 cttttttgac tagttgtgtt ttttcttccg gagtacagct tgcatagtaa tgttcaatcc
    53581 ctacttgttc agcaatggcc tttgctgtgc cagaatggtc gccggttatc atacttgtat
    53641 ggatgttttg ggatttaaag taggaaatta cttgttgggt atttgctttt ggaatatcta
    53701 gcagacctaa aacagcaatt aactgctgat ctttagaaag ataaatcacg gttttgcctt
    53761 gattttccaa acgttcgatc atttccgcaa tctctttgct gattatcatc gtctcaggat
    53821 catatgcgtg cttgccaact ttccattgcg cacctaaata ggtagtttgt aatccaaaac
    53881 cagtaatttc ttctattggt aagtattgga tttcttcagg tattgtgcaa tcgaaatatt
    53941 gcaaaatcgc atgagcaagt ggatgcgtcg ttttttgttc catagcaaca agaagttgtt
    54001 gtgccaaatc cttttcatca aagaaaaagc tgtcagttac aactggtttt cctttagtga
    54061 gcgttcctgt tttatcaaag gcaatcgctt ttaattcaga aagcttttct aaatggatac
    54121 cgcctttgat taaaatccca ttttttgcag aatttgaaag agcagctaga gaagctggtg
    54181 tagcagatgc gacaagcgca caaggagaag ccacaactaa taataccatt cctcgataaa
    54241 agctttcatc ccaactccaa cctaggaaaa agtgggggag aaggatcatt agaggaacag
    54301 caagcaaaat caattttaca taattatttt cgattcgttc gataaatgaa gctgtttgag
    54361 ttggcgtatt ctgcgcttct tctactaatt ggataatctt agcaaagaca gtttcggaac
    54421 tcgtttttgt tactcgaatc gtgatgggat ttccttgatt cagcgttcct gcatacatct
    54481 catcgcctaa atatttttct acaggcacag attcaccgct taatgcagac tcatcgatcg
    54541 ttgcctgtgt gctttctaaa aagccatcta tcggaacagc tgctccttta gcgacaaata
    54601 ccaagttatc cacctttagt tgagaaacat ctacttcttg catttgtcca ttttctttta
    54661 ataattgggc tttttgtggt tgaagattca tcaaagcagt aatttctttt tgacttttat
    54721 tagttgtgta ttcctctaat gcaccgctta aacaaaagat gaatgtcaac attgctcctt
    54781 caaagtaatt accgatcaaa caggcaccaa tagcagctaa agccatcaat aaatccacat
    54841 tcagtgtccg atcattccac aattctttca agccttccga tgtttgttta tatccaccga
    54901 tgataatggc actgatataa aaaagtgggg tgatggtaag agagaatcgg tcgaagataa
    54961 aaccgaagat cataaataaa aggcagccga ttgttgctgc aaaagctttt ttctgattag
    55021 tcatcgttat tagacctcct tgtcaaaaca aggatagcat aataaaaatg taattgataa
    55081 taataacagc taaatgagaa cgataatggt tatcatttgg tgattaagct tgatgttgtt
    55141 tttattattt ataatgatta ttattaaaga aaataaaaaa ccagaacatt tctcaaataa
    55201 gagaagtgtt ctggtttttt ctacattgca ataaaccaat aaccaatcaa aatgatacca
    55261 aggataatac gataccagcc aaaagcggta aagtcgtttt tcttcaaata gcttagtaag
    55321 aatttgatag ctaagacgga aaccacaaaa gatacgaatg ttcctacaag aagaacaaca
    55381 gtttcttcca ttttgaatga attcccttca gctaaatatt tgacgatctt caagccgctt
    55441 gcgccaaaca tgacaggaat ccctaagaaa aatgaaaact ctgtagcgac aaaacgcgaa
    55501 gcaccaatca agatagatcc taggatcgtt gcgccagaac gggatgttcc gggaacaagt
    55561 gccaagactt ggaataaccc aacaagaaga gctgcttggt acgtaaagtc attcagagaa
    55621 gtccaacgag gtgttttgtt tttgttgcgt ttttcgacaa taataaaggc aataccgtaa
    55681 acgatcaaaa caatggcaac tggtaagaac ttgtggaaat ttgcttctaa ccaatcatcc
    55741 aaaggcaaac caatgatgat ggctggtatg caagcaacga tcactttaaa ccaaagtgtc
    55801 cacgtatctt ttttttctac cgttgattta cttggagaaa atgggtttaa cttgtgaaaa
    55861 taaagaacga cgacagccaa gatcgcccct aattggataa cgacattgaa catcgacata
    55921 aattgtgaac tggcatccag tttgatgaat tcatctgcta ggatcaaatg accagtacta
    55981 ctgatgggca cccattccgt tattccttca ataataccga gaatcactgc tttgataata
    56041 tttgcaaaaa acataaattc cttccttccc aattaagata catcttatag tataccgtta
    56101 ttttttgaga aacccaatct gttttttaat attaagaaaa acttgagaaa taaagcagat
    56161 aagtacacga agagaaaaaa gaagacaaag ccctcgttca gactttgcct tttatgattc
    56221 tctggtaaaa attaaaatat ccccattata gaaacgagtc gcgactatgt gttttcccgt
    56281 tatcgatata agacgtatca ttgatgccgt ggatagaaaa tcgaccgttt gcataagtga
    56341 cctttgttac gctgccattt aaaagcccag cacgaacagg ctgtttagga tcgatcagat
    56401 taagcaaaaa ggcgatcgtc aaaccatgag atacgactaa aacatttcca ccaccatttt
    56461 tttcgcagtg atgggcgata tcttcaaatc ctgtccatac acggtctcgc aatgtttcat
    56521 aagtttccgc ccacccagcg gtatctaagt tgtatacagc attagcaatt tgttcaaaag
    56581 aaacttctgt ggtaaacatt tcatcatatg ttttaaaatt caatacacga gggatcacac
    56641 cccacatttc catgtcatat ccaccttcaa gagacccgaa acaccattct ctaatccttt
    56701 tttcaataaa ataaggaatt tcttttccct tagaatgttc tcctagaata atacgggcag
    56761 tttcgattgc acgaccactg tcactagaat aagcaaatga aaagtcgaca ttttttaacc
    56821 cgcgacctaa ataatgaatg ccttcttgtc cttgctttgt gaggggtgta tcacaccagc
    56881 cttgtactcg ttcaattgta ttgaacatcg tttttccgtg tcgtacgata taaagtgtag
    56941 tttctgtcat tttattctcc tcctaagtcg ttttaacgat tgaaaaaagg atttgtcctt
    57001 ttttcctgtt caatcgtcgt tgcatcgcca tgtcctggat agactgcaaa ttcatcaggt
    57061 aatacaaaca gctgggtccg aatactatga agtaattgtt ctaaattccc tgtatacaga
    57121 tcggttctac cgatacttcc tctaaacagg gcgtcgccag aaacgacaaa atcatcaaaa
    57181 ataaagctga cgcttcctat agaatgccct ggggtaggga cgacgcgaaa agacatgttt
    57241 cctaaagtat aagttttcat ttcaaattca tattctgcag gcgagacaat aacatcagca
    57301 atatcatcat gtctaccgag tccagaaaga ttcaaaatcg gatcacctag ccatttttgc
    57361 tctagtgggc tgacataaac tgggatttga taatacgcgc gtaaaggttc cactgcaccg
    57421 atatgatcgt agtgagtatg ggtaagcaaa atcgctaccg gctgttggtt tgtttttttg
    57481 atttcttgtt tgattcgttc cgcatcttct ccagggtcaa tgatcaaaag tgcttcttcg
    57541 ttgtagacca aataacagtt ttcttcaatc attcctgttt ttaatctttc gatatgcatg
    57601 tgcattcctc cttgatagcc aaaacttctt tcatccatta gtataacgca ttcattgcta
    57661 ggaaacaaaa acaggtactt atttctcttt acttatctga attatcagac tatatgaaaa
    57721 gtatttggat attctaaaga gaaggaaagt aagttttcat agcattttaa tatgtccttg
    57781 ttacattaga ttcatccaaa aatccaaatt ctatatccta tatccctgcc taatcagcag
    57841 ggtgtttttt tatgtttgga aagagagctc tttttgctaa actaggtttt ttcaggtaat
    57901 cttagagaga aagaggtggt ggaaatgatc aaacagtcaa aaatatctgt gcggcactta
    57961 gtagaattta ttttgcgacg tggaagtatc gacaatcgta aaaaaagtaa ccatactgca
    58021 ttagaaggag cgagaatcca ccgcaagctc caaaaagatg cgggagaaaa ctatgaaaaa
    58081 gaagtctttt taaaaacaac agttcaatta gatgaatatt acctgatcgt tgaaggccgt
    58141 gcagacggta ttttcaaaag agacggtatt tattatatag atgaaatcaa aacctccgaa
    58201 ccacgatttg aagatttaga accagaacaa gtggaacttt tctttcatca agcaagggtg
    58261 tatgcctata tctatagtca agaacatgat ttacaagaaa taaatctgca attgacttac
    58321 ttccagacgg cagaagaagt gatcacgaga aaggttgaac atcagacaag agagcagtta
    58381 gaaacttttt ttaaaaagct gactgaagat tataaagaat ggcttgtttt tcaagaaaac
    58441 tggcgcactg tacgcaatgc ctctttgatg gctttgaaat tcccccatga tgagtatagg
    58501 aaaggtcagc gtgaactagc ggtagctgct tataaaacga tccgtacgaa acagaaattg
    58561 tttgtagaag cacctaccgg aacaggaaaa acaatctcta cgttattccc cgctttgaaa
    58621 gctgtagggg aagaagaggg agaaaagatc ttttatctaa cggctaaaac aataactaga
    58681 caagtagctg aagatgctat gacagcatta aaagatacag gagctgaagt aaaaagtgtc
    58741 actttgacgg cgaaagacaa gatctgcttt ttagatgaga cgacctgtaa tccagatcag
    58801 tgcccatatg ccaatggtta ttacaatcgg attaatgaag gtttatggga tcttttgaat
    58861 catgagaatc agatcactcg tgaagtcatc gaaacttatg caagaaagca tacactttgt
    58921 cctttcgaac tatcattaga tgtcagtatc tggtgtgacg taatcattgg ggattacaat
    58981 tatttatttg atccaactgt ttatttaaga cgtttttttg aagatgaaaa aaacgaggac
    59041 tatctgtttt tgattgacga agcacataat ttggtgaatc gttcccgaga gatgtattcg
    59101 gcagaactat cttatgaaaa aacgaaacga agtaaggaag ctataccaaa ggaattcaaa
    59161 aaacttcatc gccgtttcaa caaactatta aaagaatttg attcgatccg agaaatagca
    59221 aaagaagatc attgggatta tcatcatcaa aaagcaccgg ctgaatcttt agtaaaggca
    59281 ggttatcaat taagcgaaaa aattaaagaa tggctggcag aatttcctga gcacccatca
    59341 caagaacaat tgcttcctta ttattttgat ttgcttcatt ttttgaaggt cagtgaattt
    59401 tatgatgatc actacgaaac aacagtcgaa aaaacgtatc gcgatctgat agtaaaagag
    59461 ttttgtattg atccaagctt gtttcttgag cagtcgttgg ataaaggaaa aagcagtatt
    59521 cttttttctg ctagtttttc accactctct tattatcagg aaacactggg gggacaaaaa
    59581 agtctggctt acaaactgcc gagtcctttc ccagaaggaa accagcaagt tttcatcgca
    59641 aattacctag agacgactta tcgaaaaagg gaacacagtc tggcaaaatt agtagagacg
    59701 attcatgctt ttgctgatgg aaaggtcggg aattatttcg ttttctttcc ttcttatcaa
    59761 tatttagatc ttgcggtgga agcattcaga cagcaatatc ccgacagcaa tgtgctaatc
    59821 caagaaacgg acatgaatga ggaagaacgc gaacgatttt taacaaaatt tcaaacaaat
    59881 cccgctgaaa cattgattgg attttgcgta cttggcggga ttttttcaga aggaatcgat
    59941 ttgagagggg accgattgat cggcagtatg atcgtaggtg taggtttgcc gcagatgaat
    60001 catgagcagg aactgatcaa atcatattat gatgagaagg aacaccttgg atttgcttac
    60061 gcttatcagc ttcctggaat gaacaaagtc ttgcaagctg gtggtcgagt gattcgtggc
    60121 atggcggatc aaggaatcgt tgtattggca gatcaacgat tttctagttt ttcttatcga
    60181 cgtttattcc cacaacattg gcaaacagca agagaagtac gaagtgttgg ggaattggaa
    60241 gaaggaatac agcgtttttg gttatcaaaa gaagcagtaa atcaaaaaaa cgaggaatga
    60301 cagacgtcag cctcgttttt tgattaacga tagttagtga attgcaactc gattggtaag
    60361 tcaagtttac gtaataaagc aatcacagct tgtagatcat cacggttctt acctgtcact
    60421 ctaatctgat cttcttggat ttgtgctttt actttcagtt tttcattttt gatagctgta
    60481 gtgattttct tcgcgttttc acgatcgatt ccgttgacca aatcggcagt ttgtcgagct
    60541 ttgccaccta gagcgtgttc tgattcggaa aaatggatat ttttgattgg cacatttctt
    60601 ttgatcagct taccaaataa tacatctttg atctgttcga ttttgaaatc atcatcgctc
    60661 aatacaacta gattgctgtt ctctaatttg atttctactg tagaatcctt gaagtcaaag
    60721 cgattggaca attcttttgt tgtttgctga atcgcatttt ttacttcttc tgtattgact
    60781 tctgatacaa tgtcgaagct agcatctttt gccataggaa ccctcctgat atacatataa
    60841 atatttacca tacatctatc ttatcatagg attagggcag attcttacct gtttgctaaa
    60901 attcttctca tttgagttcg aagtatttta ttttaataga taaactgtta ttcttacatt
    60961 tggaatacca aagtagggga gaggtgcttg tgagtaaaat tatgactgcg tatcataaaa
    61021 atgaaagatt aaagaaaata gtggttattt tgataaccgg attgaccgct gcggtcgctt
    61081 taaattattt tttgattcca gccaatgtct tttctgcagg gatgaatggg atcgctcaaa
    61141 tcatttcctc gcttttatat gaatggtttc atatcaagat agacacagga acatttattt
    61201 ttcttttgaa tatccctgtg tttgtattag ggtttctcaa agtaggaaaa cgagcaacga
    61261 tccttagttt tgtgaatgtt gtatgtgtat cggtgatgac gatgctgctt ccagtcggac
    61321 agatcactga taatattttg atgaatgccc tagtaggcgg tgttttatta ggtcttggcg
    61381 taggattatc tttaaaaatg ggctttacaa ctggtgggat ggatatcatc tctcttgtac
    61441 tttcacaaac aactggaaaa acagtcggaa actacatgct gattctaaat ggtatcattg
    61501 tagtcatcgc aggcttcttc tttaactggg aaagtgcctt gtatacgatt atttccattt
    61561 atgcaatgac tcaagtaatt gatgccatcc acacgagtca tcaaaaagta acagcaatga
    61621 tcgttaccgt caagccagaa gaggtaacag aagaactagc aaaacggatg gtacgtggaa
    61681 tgacattact gccgtctgtc ggtggctatt caaaaaaaca agggaacatg atcatgatgg
    61741 tcatcacccg ctacgaaatg tatgatcttg atcagatcgt tcatgaaatc gacgacgatg
    61801 catttatcaa tattttacca actcattcag tatttggccg gtttgcaaat gaaaacgaac
    61861 aaaaaatgta tcgaacaact ggcgtattgc cagaaataaa aaatcgttca caaaaaaaat
    61921 gaggaatcag gtttatttta gaataaattt aatctattac atctaataag aacattttta
    61981 taaatttaaa cgagaatctt acttctaaat tctcatttac aagcagctaa aaaaagagta
    62041 tgataaataa aaatcgtgtt aaggagtgtc gtacgatgag cgaagcaaac caagaacggt
    62101 ctggacaaga aatagaacta atcaaagaac gtattcttgc tgccttggaa atggtgattg
    62161 atcctgaatt aggaatcgac atcgtcaatt taggcttgat ttatgatata gaattcaatc
    62221 ctgaaaatgg agaaacagtc attaagatga ctctaacaac tatgggctgt ccattagcag
    62281 atattttaac agaatctatc catgacgcat taaaagaagt gccagaagta acgaaagcag
    62341 aagtcaaact agtctggtat ccagcatgga cgacagataa gatgagccgt tatgcacgga
    62401 ttgcgttggg gatacgatag aaatcttggc tgactagcta tgagaagcaa ataggaaaaa
    62461 caaaaaagcc ttttatcctt tctagaagaa aagagataaa aggtttcttt gtttataaaa
    62521 ttttgtctgc tatattataa gttaattaaa taaatgtgtt ctattgataa aggacattca
    62581 aatccctttc cttttggaat ccttcttttt tacatcagtt tgattttcgt atagtattct
    62641 ttctcatctt cacgataaat cgcagcccca tttttttgtt gtgctttaag actgccaaga
    62701 ttttttttat ttacagacaa aagtgcctca tcaatgccga ttttttttgc ctcttgtagt
    62761 actaatgcta atccttgtgt ggcatatcct tttttacgat aattagggga gatgccgtaa
    62821 ccaatatggc cagcgccgtt tcttaatgtt tcgttcagtt tatggcgcaa attaaaaatt
    62881 cctacatact catgttcatc actttctaaa ataaagacgg ttgcgggaac gtaactctca
    62941 ggaagatcga ttcctaaaga cgttttttcg cattttttta cataatctaa aaattcagca
    63001 aatgtatagc cataagcatt gttgataaat ccactctcat ttggtccaaa gctagtttgt
    63061 agctgataag ctttctttaa atctgcttcc catagtttga tcagtttcat cgtattcacc
    63121 tctttaagta accttattca tttgttttct actatagcac tattgttgta aatagccatg
    63181 cctattttta tttattgtgt catatcgtat taagaatcat cgaacaatac aaatatgaat
    63241 tacaataaaa aaattatttg aagttcgttc attcgttact tatgatctct agaagaaaga
    63301 aaacgtgaat tacttcagcg tctagaattc cagaaggata gtaatcaaca gaagaaataa
    63361 tcaatatcca aatgaaagaa gatagctgaa tcgagaaaaa gaggaaaaaa gaaatgaaga
    63421 aaaaaataaa atagttacta atcccagagt cgttcgttta acattgaaat cgatcatatg
    63481 gataaaacag attttttcac caaccctcaa tagatgatgg agttgctcgg atgaattatt
    63541 tgtgtagatg gcataaggga gggcgtacag aaatgaaaaa gaacaaaaat atgttcttga
    63601 caaaaaacac atgtaatcct aaactaaaag atgtgatgcg gctatggcgg aattggcaga
    63661 cgcgctagct tcaggcgcta gtgagggtga cctcgtggaa gttcgagtct tcttagccgc
    63721 ataatttgtt tttttataaa gagaaggtac acctggagcg atgagaaaga aaataacata
    63781 ttatctaaaa gaatatagga aaaattttaa acaacggaac cagattcaaa taaatcaaaa
    63841 aaaatttgtg tgaaggtggg acagtttaaa acctaataga ttgattcaaa tagtagattt
    63901 atagattctg atacaagaaa tactggcaaa cgatagttaa ggaataaaga ctaaacttaa
    63961 actcaaaaag aggtcatgcg aaagaataaa tcgcatgacc tcttactata ttttgaatgt
    64021 gtttaaacgt tcaaataatg aatcttagta ccaaccgttt gcttcccaga atgctttcgc
    64081 cgcttcccaa gaaccataac gtgaagtaac atattgttct gctactcttt cttggtttgc
    64141 agcagagtag tcaccattta agtaagaaga atctaattgg taacggccga tgtaacgtcc
    64201 gttcgttgct gtgtaagaac cgcttgattc tttttgtgcg atccattctt ttgcagagct
    64261 tgtgcttgct ggagtagcag cagtttctgt tacagcagga gcaggttgtg tttcagcaac
    64321 aggcgctgct tgtttttccg tttgaaccgt ttcttgtaca acaggttgtt cagcttggac
    64381 tggtgcttct tgaacggctg cttgaggttc agcagctgct tgtgcagagc cttctgttgg
    64441 gattgttaat tgttgtcctg agtaaatcag gttaatatca gaaattgaat ttgattctgc
    64501 aatggcattg atcaaggagt tatcaccaac gtatttttga gaaatcgttg atagagtatc
    64561 acctgattga acggtataag cttcgtccgc atgagcagtc gttcccatga agaatgcagc
    64621 gccggcagcc aaagttgttc caaaaagtaa agttttaagt gatgtcatga aaaatctcct
    64681 ttattcgtta aaaagtttta tttgttgtgt tcaacgagga atactttaac acgtgaaaat
    64741 atttcatagg taaaagaagc atgacaattt atgacaaatc cacgtttttt gtaacagaat
    64801 gatgttcgct cgatgtgaaa aatgatgagg aatttcacat aatgcagaaa taatgggatt
    64861 agaacaggtg ttctgatgag aaaagaaatg tataaataac ttgaaagaaa ttgcttttgt
    64921 tcatcatttt atttacatat tgcgtatatt ttttgtattt ttgcttgaga tgttacaata
    64981 aaagaaaaag gaggcggttc caatgagtaa aagataccag aacatcatgg tagcagttga
    65041 cggatcaaga caatcgatca aagcgttcgc tgaagccctt gacttagcaa aggacaatga
    65101 agcccactta tttatcgtgt caatcatcaa caaagttgag ctgacccata gtgcctatgc
    65161 cttttcaaaa atctatgcag atgaaaaaca aagaacagaa gtagccatgc tgaaaaaaat
    65221 caatgatgcc aaagagtacg gcattgctga gatccacgca atagttgaaa ctggagatcc
    65281 aagaaatttg attgccaatg tcatccctgg acaagaaaat attgatttga tcgttatggg
    65341 agcgacagga aaaggtgcta tacaacaagc attagttgga tcgaccgctt cttatgtcgt
    65401 atcacatgcc ccttgcagtg tgttggtcgt gaagtaatga gataagggag gaatagaaaa
    65461 tggttcgtcc tcaagaagtc cgtgcgccaa aagaaaaaat agaaatttta gcaattattg
    65521 aagacggcac acaaacaaag aagggatact cgatcgcttt agtaaagtgg aaaggaaaaa
    65581 aaggtgttgc tctgagatgg gatggagata accaacaaga taaaggattc cctgtcacag
    65641 ctaacggtta tcatccagcg tggtttgttc tgcctgataa atttatggaa ttatacagct
    65701 atgattatgc aagagccgca gcatcaatca aagcattaga taaattaaca gaagagcaga
    65761 atgaaatgga ggaaaaataa aaaagacgaa ccaatcagcc tttagaaaac tgattagttc
    65821 gaggagtaaa aatgaaaaaa ggttatttgg gatgaaacta gaataatgcg tataacttaa
    65881 ataaaactta aagcaataaa atggcaagag ggtgaggcaa atggaagcag gaaaagtatt
    65941 acaggaagta aaagcactat gtgcagataa aaaatatgaa gaagcgaaaa gcctgatcga
    66001 aaagaacaaa gaaaatttgg aagaaaagta cccgatcgca caagaattta tagaattaaa
    66061 acaaggaacg atccttcacc gattcaaaca tttttttggt ataacagaat agaaaaaacg
    66121 attaaagatc ggcagaaatt tgctgcatct ttaatcgttt tctttttaaa catgcgaggg
    66181 attcaattct gattcttata taaaaaatag atccaaataa taagaaaaat tactgaaagc
    66241 gtcaattcga tgatgtgtgt tattaataca gacgttgttt cccaatgcct aaaagaaaaa
    66301 atttgataag taaaatatac agcaggtaaa gaggccagta aaactgaaaa cttctttttt
    66361 gtccatttta tgtaatagaa aagaatgctt atcataataa aaaaaggaat ataaaaaaca
    66421 aattgaccga tcgcgatcat actattgcct ccgataaata aagtccatga tacaaaataa
    66481 cactttgtgc gaaatggcgc aagcagtttt gtggatttat ccggtaaaat ctgtatcgat
    66541 tttgaaatta gaggagaaac ttgctataat caaagtgaaa gaaggagtgg gattttcatg
    66601 gatcatgtag tgttagaaat cgatttgcat ttgatcaata atattcgggt gatttactat
    66661 gttgtaagta acagtgtcga acaacgtgta ctgacgaata aaataaatgg tattttagca
    66721 aagaaagacg ttcaccgatt caataatggt gaaggaagtt attatagtat cccagtagaa
    66781 aaaattattt atacgacagt caaagtcaga gaagatctag ataccaaaaa agcatatgaa
    66841 cctattttta caacttattg attgacagca gatggaggaa aaaaatgaat ccatatgaac
    66901 aacttattca agatttgatc gatggaaaaa tcgaaaagat tgatgtaaaa agagaagaat
    66961 taatggcttt tcgagaagcg tggctgaagt tggagaatcg taaatatgtg gtgggaaaag
    67021 cctcactgaa cggcaatgtg acttatcatt acgatccgac acgtttattt tagtatgaag
    67081 tattcagaaa agaaccaaag gaggacgatg cagcagcatt gtcctccttt gattttacaa
    67141 aagtgaaagt tttacttatt tgaatagatt tttaaatttg tttttgacac tgtcgatatt
    67201 tacatctttt aaaagatttt gggctttctc tttataaggt cctaattgtt ctttatgttc
    67261 ttcgataaat cgttttgcgc catcgaaatt gccgttctta atcatttcct tgactttttg
    67321 aaataattct tcttgattca ttttgaacac atcctttgct atcaacatga ttttttacat
    67381 ggacaaaacg agaaaaactg atttatccac acgtcaataa tatcaaataa cgatcatttt
    67441 tactaaaaaa acactcgatt ctcgcttcta aagtaggaga aaaatctcca acttgggacg
    67501 gatggaattt ctttaactga cccatatact gaatatataa caaggaggta gaaagatgaa
    67561 agtgattaaa tatttgattg gactggcggc gattagtggt attttgctat ttggactgaa
    67621 atattatact gaaggaagtt atggagaaat ccctggtata cttgatcagc taaatccatt
    67681 agttgaaaaa ggagaggtct atgtaaagac acaaaaacca gaggaagtaa atgaatatgg
    67741 tacagcaagg tatgtgcaga aagctgctga tgccaatggg aaagaacgaa cggttgaatt
    67801 taatggtctt tctgtgttaa aagaaaatca ttatttgaaa attagcaata aaggagcaca
    67861 catagaaacg tatgaggaag ttcctagaga aaaagttccg gagaaagcat tagcaattat
    67921 tgggtaaatc agtttttgta ttaagaatac gcccgatttt attattcata atttaattat
    67981 gtaaactaaa ttctagtagt aaaaataata caataatccc cctgcaaaaa gattgttctt
    68041 ttacaggggg ttcatttttt cagcaagcac atcaaattgg gatggcaggg ttcgaaccta
    68101 caattgcttg attcagagtc aagtgcctta ccagtttggc tacatcccat ttattcaaca
    68161 aaataatcat agcactttcc taaaaaaacg caagttattt ttcttgatcg gatagttgtt
    68221 gctttactcg taaaaggcgg cgtactagtt tcttttctaa tccagcagga agggcaatca
    68281 gttctgctac tgacaagacg gtgtccaata actgatattc tttgacaggt tcattcataa
    68341 tatgatagat gtttgctaaa cgttcaatat attcaaagcg agtatactct gttcgagcaa
    68401 ttagaaattt caataaatcg gtggctttct cgtactcttg atctgcgtaa taaacaagag
    68461 attgacgata caatttgatt tccagttcat tgagggtagc aggtctgcga cgctcgttcc
    68521 actcattaat agagacttta ccttgaacat ttcccttgca gtcacggaca aaaataatcg
    68581 gaatttcatt tggatccatt tttttatgtg tgaatttttc cattatctac gctcccttca
    68641 aaggtttggt atcttgagta taagggattt ttgtttgaat tactagcttt tctccaaagt
    68701 tagcatctgt ccacccattg cagtgaaaat cgccgaaaaa tcgttgatcg gtactgctcg
    68761 attttttaca ccatagggat cgttgacata aatattttct ttgtcatatc cagtgactac
    68821 acaagcatga acaagaggag tgactcttac ttctccatta tttgtggtcc aagtcataaa
    68881 atcagtttca tctgggactt caaaattgat agtcacagaa gtccagacag gatttccttt
    68941 agatagtgct tttttgatat catcgagttg agcatcggtt tttggaaaag tgtgataatc
    69001 cgtttggacg atttgctgag caagggaggc aactggggga acatagacga ccatagccca
    69061 gtctccgcca ctgatatcac caacaaatcc ttcattagga tcgccatgag tcccatctgc
    69121 attgaaaaca gggacgtaat ccaattgctc agctaactgg tttttagttg tttcataacc
    69181 ataaaaagat aaaagcatag aaagcgaagt gacttcacag ccattttcta aagaaggaga
    69241 atcaaactgg ctctctaatg gaacatctag taaaacttct gctttctctt ttgtcttttt
    69301 cgttttttgt gttgaacttt tttgtgcagt gttcattgta tgttctggag aatggatatg
    69361 taggtaataa cttgtaccgc ttaataaaag agcagctgtg ccccctagaa acaatagttt
    69421 tttgaacgtt tttttcttca tttgaaatct cccttttcag
//
LOCUS       DAZKCY010000003        60602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000003 DAZKCY010000000
VERSION     DAZKCY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.003"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..554
                     /locus_tag="ACG8XC_000159"
     CDS             234..554
                     /locus_tag="ACG8XC_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFP9189892.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            715..1620
                     /locus_tag="ACG8XC_000160"
     CDS             715..1620
                     /locus_tag="ACG8XC_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9189893.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1617..2390
                     /locus_tag="ACG8XC_000161"
     CDS             1617..2390
                     /locus_tag="ACG8XC_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9189894.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(2567..3991)
                     /locus_tag="ACG8XC_000162"
     CDS             complement(2567..3991)
                     /locus_tag="ACG8XC_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFP9189895.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4202..4648
                     /gene="nrdI"
                     /locus_tag="ACG8XC_000163"
     CDS             4202..4648
                     /gene="nrdI"
                     /locus_tag="ACG8XC_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9189896.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(4718..6700)
                     /locus_tag="ACG8XC_000164"
     CDS             complement(4718..6700)
                     /locus_tag="ACG8XC_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFP9189897.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6714..7610)
                     /gene="murQ"
                     /locus_tag="ACG8XC_000165"
     CDS             complement(6714..7610)
                     /gene="murQ"
                     /locus_tag="ACG8XC_000165"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9189898.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(7613..8674)
                     /locus_tag="ACG8XC_000166"
     CDS             complement(7613..8674)
                     /locus_tag="ACG8XC_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP9189899.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8886..9413)
                     /locus_tag="ACG8XC_000167"
     CDS             complement(8886..9413)
                     /locus_tag="ACG8XC_000167"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9189900.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(9480..10982)
                     /gene="eat(A)"
                     /locus_tag="ACG8XC_000168"
     CDS             complement(9480..10982)
                     /gene="eat(A)"
                     /locus_tag="ACG8XC_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP9189901.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(11240..12361)
                     /locus_tag="ACG8XC_000169"
     CDS             complement(11240..12361)
                     /locus_tag="ACG8XC_000169"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9189902.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            12487..13428
                     /locus_tag="ACG8XC_000170"
     CDS             12487..13428
                     /locus_tag="ACG8XC_000170"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9189903.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13571..14467)
                     /locus_tag="ACG8XC_000171"
     CDS             complement(13571..14467)
                     /locus_tag="ACG8XC_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP9189904.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            14749..16089
                     /locus_tag="ACG8XC_000172"
     CDS             14749..16089
                     /locus_tag="ACG8XC_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9189905.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16284..16727
                     /locus_tag="ACG8XC_000173"
     CDS             16284..16727
                     /locus_tag="ACG8XC_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFP9189906.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(16989..18407)
                     /gene="nhaC"
                     /locus_tag="ACG8XC_000174"
     CDS             complement(16989..18407)
                     /gene="nhaC"
                     /locus_tag="ACG8XC_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP9189907.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(18811..20034)
                     /locus_tag="ACG8XC_000175"
     CDS             complement(18811..20034)
                     /locus_tag="ACG8XC_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9189908.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20066..20905)
                     /locus_tag="ACG8XC_000176"
     CDS             complement(20066..20905)
                     /locus_tag="ACG8XC_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9189909.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(20922..21602)
                     /locus_tag="ACG8XC_000177"
     CDS             complement(20922..21602)
                     /locus_tag="ACG8XC_000177"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP9189910.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(21604..22677)
                     /locus_tag="ACG8XC_000178"
     CDS             complement(21604..22677)
                     /locus_tag="ACG8XC_000178"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP9189911.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     misc_binding    complement(22736..22974)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23017..23250)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23327..23998)
                     /locus_tag="ACG8XC_000179"
     CDS             complement(23327..23998)
                     /locus_tag="ACG8XC_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9189912.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KKWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(24086..26734)
                     /gene="mgtA"
                     /locus_tag="ACG8XC_000180"
     CDS             complement(24086..26734)
                     /gene="mgtA"
                     /locus_tag="ACG8XC_000180"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP9189913.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASPNGLSEEQIERSRET
                     YGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELITGEKI
                     SEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGKFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLMPLPGNYWLWF
                     FITVVAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27077..27251)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27424..27861
                     /gene="ndk"
                     /locus_tag="ACG8XC_000181"
     CDS             27424..27861
                     /gene="ndk"
                     /locus_tag="ACG8XC_000181"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP9189914.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(27883..28749)
                     /locus_tag="ACG8XC_000182"
     CDS             complement(27883..28749)
                     /locus_tag="ACG8XC_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189915.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(28983..31061)
                     /locus_tag="ACG8XC_000183"
     CDS             complement(28983..31061)
                     /locus_tag="ACG8XC_000183"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9189916.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            31554..32771
                     /locus_tag="ACG8XC_000184"
     CDS             31554..32771
                     /locus_tag="ACG8XC_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9189917.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(33230..33853)
                     /locus_tag="ACG8XC_000185"
     CDS             complement(33230..33853)
                     /locus_tag="ACG8XC_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189918.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(34009..35406)
                     /locus_tag="ACG8XC_000186"
     CDS             complement(34009..35406)
                     /locus_tag="ACG8XC_000186"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9189919.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(35428..36399)
                     /locus_tag="ACG8XC_000187"
     CDS             complement(35428..36399)
                     /locus_tag="ACG8XC_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9189920.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(36406..38706)
                     /locus_tag="ACG8XC_000188"
     CDS             complement(36406..38706)
                     /locus_tag="ACG8XC_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFP9189921.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(38708..40033)
                     /locus_tag="ACG8XC_000189"
     CDS             complement(38708..40033)
                     /locus_tag="ACG8XC_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9189922.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            40264..40689
                     /locus_tag="ACG8XC_000190"
     CDS             40264..40689
                     /locus_tag="ACG8XC_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189923.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(41187..41888)
                     /locus_tag="ACG8XC_000191"
     CDS             complement(41187..41888)
                     /locus_tag="ACG8XC_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9189924.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(41904..42344)
                     /locus_tag="ACG8XC_000192"
     CDS             complement(41904..42344)
                     /locus_tag="ACG8XC_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP9189925.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTITSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(42344..42919)
                     /locus_tag="ACG8XC_000193"
     CDS             complement(42344..42919)
                     /locus_tag="ACG8XC_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFP9189926.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(43330..43836)
                     /locus_tag="ACG8XC_000194"
     CDS             complement(43330..43836)
                     /locus_tag="ACG8XC_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFP9189927.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            complement(43847..44359)
                     /locus_tag="ACG8XC_000195"
                     /pseudo
     CDS             complement(43847..44359)
                     /locus_tag="ACG8XC_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(44498..45199)
                     /locus_tag="ACG8XC_000196"
     CDS             complement(44498..45199)
                     /locus_tag="ACG8XC_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9189928.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(45307..46749)
                     /locus_tag="ACG8XC_000197"
     CDS             complement(45307..46749)
                     /locus_tag="ACG8XC_000197"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9189929.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(46763..48628)
                     /locus_tag="ACG8XC_000198"
     CDS             complement(46763..48628)
                     /locus_tag="ACG8XC_000198"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9189930.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(48749..49600)
                     /gene="licT"
                     /locus_tag="ACG8XC_000199"
     CDS             complement(48749..49600)
                     /gene="licT"
                     /locus_tag="ACG8XC_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFP9189931.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(49910..50623)
                     /locus_tag="ACG8XC_000200"
     CDS             complement(49910..50623)
                     /locus_tag="ACG8XC_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFP9189932.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(50691..51017)
                     /locus_tag="ACG8XC_000201"
     CDS             complement(50691..51017)
                     /locus_tag="ACG8XC_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP9189933.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(51140..51421)
                     /locus_tag="ACG8XC_000202"
     CDS             complement(51140..51421)
                     /locus_tag="ACG8XC_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9189934.1"
                     /translation="MSLILGVEKQANPYDHKKMLTQQLRELEQDESIHRKAYNQVPPK
                     VEYSLTEEGKSLREILVALSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(51688..52941)
                     /locus_tag="ACG8XC_000203"
     CDS             complement(51688..52941)
                     /locus_tag="ACG8XC_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP9189935.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            53002..53742
                     /locus_tag="ACG8XC_000204"
     CDS             53002..53742
                     /locus_tag="ACG8XC_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9189936.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(53828..54586)
                     /locus_tag="ACG8XC_000205"
     CDS             complement(53828..54586)
                     /locus_tag="ACG8XC_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9189937.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(54583..55047)
                     /locus_tag="ACG8XC_000206"
                     /pseudo
     CDS             complement(54583..55047)
                     /locus_tag="ACG8XC_000206"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(55066..55563)
                     /locus_tag="ACG8XC_000207"
     CDS             complement(55066..55563)
                     /locus_tag="ACG8XC_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFP9189938.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(55658..58798)
                     /locus_tag="ACG8XC_000208"
     CDS             complement(55658..58798)
                     /locus_tag="ACG8XC_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9189939.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEIT"
     gene            complement(59022..59906)
                     /locus_tag="ACG8XC_000209"
     CDS             complement(59022..59906)
                     /locus_tag="ACG8XC_000209"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9189940.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(60001..60438)
                     /locus_tag="ACG8XC_000210"
     CDS             complement(60001..60438)
                     /locus_tag="ACG8XC_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189941.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        17493 a        12865 c        10452 g        19792 t
ORIGIN      
        1 tgaaattgca aagcattcat cttcttttct gctaacgata tatccgtcaa tgcttgttac
       61 actttactaa aaagtatgag attgccgata tctggatagg tgtgctatac tacagaaaaa
      121 gatatacgat ctgccaccgg gtagtaaaaa agaggatgat cattccatta ggtctttgaa
      181 gggatgattc tcctcttttt gtgtattttt tatattgaag gaggaacgta tgtatggcat
      241 ggatggaatt aatcatagca ggaattttgg aagtattctg gtcaacgatg atgaaatgga
      301 gtgatggttt ttctaaaatc aattattctt tttacacagt gattggaatg attgctagtt
      361 tttacttttt gtcaaaggca atcaaaagtt tgcctatgag tctctcttat cctatttgga
      421 cagggattgg cgcagttggt tcggtcatta ttggtgtggt cttgtttcat gataagctga
      481 atctttccac ttggttcttt gtcgggttgc tgctcatcag catcattggc atcaaaatca
      541 ctagcggtca ctagatactt agacaagcag acaaggactg atcagaaaaa gcggggcttt
      601 ttatttgata ttcctctttc tatgaaaaaa caagaaaata agactaatga cctagcgact
      661 tctctactca tcaggtgtat gcttgtacta tctatagaaa aggagtgaat atttttgaaa
      721 agtctttcgg tccaagaatt atccaagaac tttggtcaca aacaagccct cagcgatgtc
      781 agttttagct tatctcctgg caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa
      841 acaacgatca tgaaagctat tttaggactg atccattact cagaaggaac gatccgtatt
      901 ggtgaaaatg agatttctcc ctctagtcat aaaggattag aacaagtagg agcattgatc
      961 gaatatccgg gtatctatcc attcttaaca ggttatgatc atctgaagtt attctctgaa
     1021 acaaacgatg tatctgctat cgacactatc gtgaaacagc tcaagatgga aaaatacatt
     1081 cacaaaaaag cgaaatccta ctcgctggga atgaaacaaa aattgggaat tgctcttgcc
     1141 ttactgaacc atccgtctat cgttatttta gatgagccga tgaacggttt ggatccacaa
     1201 gcaacaagag atgtgcgaaa aatcattacc gacttggcta ctcagggcac ctctttcttg
     1261 atctccagtc acttactcag cgagcttgaa aaaatggtcg atgagctgat cctgatcaat
     1321 caaggaaaaa tcatcaaaca atgtacaatg gctgaattga accaaacagc gcaagatttc
     1381 atcgttatct ctacgacaaa tgatcaagct gctcgaacga tcttgacaga cgctggatat
     1441 cccttggaag atcctgataa aatcaagctt ctaaaaaaaa acgaagagtt attaggaact
     1501 gttatccagt tattgtctgc agaaaatgtc catgtgacag atgtccagca tctgacgaat
     1561 gatttggaat cttccctatt ggaaattctg gattcagtaa agggggaaga agaaaaatga
     1621 cgttgcttat aaaacaagaa ttatttaaat tgatcaagaa aaagtcaact gctgttttat
     1681 ctgttctatt agttgctttg ctcataggaa cagcacttct agccaaaaaa tacacgacga
     1741 tcattgatcc agttgaaatg actgctcagc ttttcagtgc cacatcttgg attgtcttta
     1801 tcatgattgc tgctgcaagt acgatcattt caatggaatc acaatatggt acattgaaaa
     1861 atctccttta tcgaaagtat tctcgaggag aaatattagt aagtaaatgg atcacattag
     1921 ttatttattc tatttattta taccttttag ctattattgt caccgtgctg atgaaactga
     1981 tccttttccc ttcgatttcc tttacggaga agatttctac tggtcagaca ctgatccaaa
     2041 gcttattcac ttatactttg ggtagttata tcggtttatg gctaatttta agtttagtgt
     2101 tgatgattgc ttgttttatc aatagttccg gtgcctccat ctcagcaggg atcgtctttt
     2161 attttgcttc atcaatcata tctggtatcc tcatcgcact cattcaaaaa tgggagtgga
     2221 tcaaatggaa tccaatcagt atgctgaatc tgcaaaacca aattggcaac gaagagatta
     2281 tgaagcaatt gactcatctt tctactaatc aaatgctgtt tggcaattta gcttacattg
     2341 ctttgttctt agcattggga tacctcgtat tcaagcgaaa aaatgtatga tggttcatta
     2401 acaaagaagt tgtgacagaa gtggccagtt tcaagaaatt tcggcttatt tttagaagaa
     2461 gctacttctt gaacaccatt tattcaaaaa aagaagcgat gacacgaatg ttgtcaccgc
     2521 ttcttttttg aataaaccac aggcttcaca gcctctcttt atacttctat acgtgaagca
     2581 tcccagtggc aaataaaacc attgcgatta ctccgaccat cgtcacaccg ataagtaaaa
     2641 tcttatctaa gtggatatgt tgtacaggtt cgttttgttc tttacgtgct ttgaggtaaa
     2701 aataaatacc tggtaagaaa agaacaagaa caagcatcac ttcattaaag gctacaacta
     2761 gcatggccca tacttgaaaa gcacttgcta aaaaccctac taaaaattgg acccactctt
     2821 tccgctgata agaaagcttc atttggtaca gccctaagaa aagccacgtg aagaaaatcg
     2881 ctgctgtacc aagtgtataa gcaaagttat atgcactgtc agtgaataat aatgaaagta
     2941 aaaagacatt cgtacataac gtcgtcaata cgatagcata agttggtgcc ccatttttgt
     3001 tgagttttcc ccatgcttta ggcaaaagtc cgtcctcagc catattcccc attgtttcaa
     3061 tcggcaagat tgtccatgag agccaaaccc caactaaaga aatgatcaaa ccgccgttga
     3121 tcacgaccgc cccccattta ccaaccatca tttcaaatac atatcccata gcaggttgcg
     3181 ctttgatcgt ttgcagctga ttcatcgtca ttgtgccata tggaagcatg gaaaccaaga
     3241 cgtaaataat gatcaaaccg aatattccta gaatcgttgc tttcccaaca tccgattgtt
     3301 tttttgctcg tgaacctacg acagaagctc cttcaatacc aacgaatacc cagagcatca
     3361 ccatgatgca actcaatact tgactagaaa acgggatcgg ctgcccattc gcattcaata
     3421 tgatatcgct agaaaagtta ttaatgaatt gttggatcga aaaggtgctg atagctacta
     3481 caataaataa aaataatggc actaatttac aaaatgtgac gatagcattg acaaagctag
     3541 cattttcaac tccacgattc actagccatg catatcccca taagaagatc gtactcatca
     3601 cgatcgatgc cacattttga ccaccttcga atacggggaa gaaataacct aatgcactca
     3661 tcaacaatgt tgcgaacgcc acgtttccta accagcatgt gagccagtaa ccccaaccgc
     3721 tgataaaacc agcgaaacgg ccaaaacctt cttctgcgta actaaagatc ccgcctttta
     3781 aatctggacg ttttttattg atattattga tcgataagga gagcatcaac attgctgttc
     3841 ctacgagtac ccagctgaag atagctgggc caccagccga tccggagatg tccgtcataa
     3901 taccaaatac gccacctcca atcgctgaac caatcactaa ggcagcaagt ggaatcaaac
     3961 caattccttt tttctgttgc tctgtatcca tgtaagcatc ctccaaagta aaaccgcttt
     4021 tatcaaaaag tataacaaac cttttttatt tttacttaat taataaaaac tttaatagaa
     4081 atataattct accgactttt ttctcattat acaagcaaag cgcctagtcc ctttccaaat
     4141 tacaccacca aaaaaactta aaaagatgct atactaaagg gaaagaattg ggggatgatc
     4201 catgaacatt ttatatatat cgatttcagg aaatacgcgt gcttttgcca aacatctagc
     4261 agaatatgct gaaaaaatgc atgcagaaga tcctgtcaat cctgaagtta ctttaaaaga
     4321 aatccatgaa aatagtgatt ttacgaaaga aacagaacca ttttttactt tcgttcctac
     4381 ttatttagac ggcgggaatg ggttagacaa cggtgacaca gaaatcttga ccgaaacgat
     4441 gcgcgaatat ctggaatacg aagacaatca taaactttgc cttggtgtag tcggtagcgg
     4501 taataaaaac ttcaacaatc aatattgtct gacagccaaa caatacgcac aaagattcgg
     4561 ctttcctttc ttggcagatt atgaattacg gggaacacca tctgatgtga cacgcgttta
     4621 tcaaattttg gcagaaaaaa ataaataaca caaaagaaga agctgaaaaa taaatttcgg
     4681 cttcttcttt ttatgttctc tattgtttct attcttatta agctttgaca tccatcacag
     4741 gtgcttttgc tttcgcttta cctgtttttt ccaagacaaa gttttttaca cgatccatat
     4801 tggttaccaa gacgatcatg gaggtttctt tgtctgctgc tttgattgct tctaagtcag
     4861 cttctgctac aagcgtatca ggtgtcactt gctgtccttc tgttactttc acatcgaatg
     4921 gcgtaccgcc taagtcaact gtgttgatgc ccatgtgaag caagatttct aagccatttg
     4981 gtgtcgtaat gccgatagca tgttttgttg ggaaaacaga acttattact ccagtaaccg
     5041 gggaatagat atgtccgttt tctggttcta caaagtaacc ttcacccatc attttttgtg
     5101 caaaaactgg gtcacttgct ttatctaaag aaacaactgt tccgtcagca actgatgata
     5161 agatagtcac actttcagct gaagcttgag gtactgcttt ttcaggtact tcatttactg
     5221 tggctgtttc ggtcgctagt tcttcttttt ctaaacggtc tttaggaata ccgaagaaga
     5281 atgtagcaac gaagccacca gcgtaagcag ccaataaacc aaggatatag cctaaccatt
     5341 taccatcaga gatcaatgga atcaaagcgg ctcctgaagg tccaatagcg attgctccga
     5401 catttccgat cattccgatc actgccccac cgatacctcc accgatacaa gcagtaataa
     5461 atggacgtcc tagaggtaat gttactccgt aaatcaatgg ttcgccgatt cctaagattc
     5521 cgactggtaa agcccctttg atcatttctg ttaattcttt gtcttttttg cagcggatcc
     5581 ataatgccaa tgctgcaccc acttgtcctg ctccggccat cgcaaggatt ggaagcaata
     5641 aggtacgacc agtttcatca atcatttgta catggattgg cgtcaagatc tgatgtagtc
     5701 cgaacatgac cattggcaag aataatgtac ctagaacgaa gccggcaaat gctcctccaa
     5761 caccaagtac ccagttgatc ccaccgacca tgccgtcaga aatgaaccct gctaaaggca
     5821 tgatgaagaa aatttcgatc agaccgatgg caatcaggga aagagttggc gtaacgatga
     5881 tatctaccgc atcaggtact actttatgca gttttttctc aacgattgac aataaccaaa
     5941 ctgcgaagat aacacctaag atcccgcctt gtccagttgc aagagcttca ccggtaaaca
     6001 agtttttcag tggtgcttct ggattcatcc ctgtcagcat cacaacagcc ccgataactc
     6061 caccaagcgt tggcgttgca ccaaaaactt gtgcagcatt gatacctgta tagataacta
     6121 gatagctaaa catcccgttt ttcaagatgt tcatcacatc gatatattgt tgccatgttc
     6181 ctgcgtctag tgttccagct gtgactaagt ttgctaaaac tgcggcaatc cctgccacga
     6241 tcccagtgcc gacaaatgca gggatcattg gtacaaagat attagagatg tcttttaata
     6301 ccgatttgat tttagagggt ttttgtttcg atttttgtgc cgctttcatt tcttgtgccc
     6361 gttcgtttac acggtctttt ccagatgcag aagaacttgc tgtagttgca ctagcagaag
     6421 gaagtttttc tcctaatttc acgcctgcta agtcaaccat ttcctgcgct actttgttca
     6481 ctttcccagg tccgatgatg acttggagct gttcatcctc tactacgcca agtactcctg
     6541 gaatcgcttt taattccgag acatttacaa gatcatcatt gatgaccttc atacgtacac
     6601 gagtcatgca gtggacaatg ctttcaacat tgcccatacc gcctgctcct tgataaattt
     6661 gttttgccag ttcttccaaa gtcaattcgt tttttgccat ggtcttttcc ccctcataag
     6721 gttcctttta caaaaccgtt tgcttcagct aattttgctt gagctgcctc tttatcacta
     6781 tttgtcaaaa tcatgacgat agccagtttg acattttgat ctgcttcctc aaagtacttt
     6841 tcagctgttt cgtagtcagc ttctgtcgct tgcatgatga ttcgttttga gcgttcaatt
     6901 agtttttcat ttgttggttt gacgtcgacc atcaagttat tgaatacttt accgataccg
     6961 atcatactga tagtagaaat catattcaaa atcagttttt gtgctgttcc tgacttcaat
     7021 cgagtagagc ctgtcaaaaa ttctggtcct gcatcgactt cgattgggaa atccgcatgc
     7081 tcgctgattt ctgcttgttt attgcaagaa atcgttcctg taacagcacc aatcgatttt
     7141 gcgtaatcta atccaccaat cacatatggt gtacgaccgc tagctgcgat accaataact
     7201 atatcatttt ttgttaaatt cagcttgatt agatcttctt gccctaattc ataagaatct
     7261 tcggctcctt cgacggctac agtcattgct tcttctccac cagcaattag gcctatgacc
     7321 atttccggtt ctacaccgaa tgtcggtaca cattcagcag catctaatac accaagtcta
     7381 ccactcgttc cagctcccat ataaatcaag cgaccacctt ttttaaagga ttcaatcgtg
     7441 ttggcgatca ctggctcgat acttggcagt tctttttcaa cagcagcggc cactttctga
     7501 tcttctttgt tcatgagttg gactgcttga gaaatactca tttcatctaa tccaaaagtt
     7561 tcttggtttc tgcgttccgt tgttaggttc tctaaattga ttttgctcat ttttatcttc
     7621 cttcttttct tataaattga tagttcatac cagctttgat ttggttgatc agcggtagtt
     7681 cttctttgat gactttggct actacattca ctttttcatc cgctggcaaa tcatatttag
     7741 ttaattggat ctcacccata tagcgcaaat acttttcgtt atctaaagta atagatcctt
     7801 tcatacgctt agctgtattt cttactggga ttggcggaat ctctcgaaaa cgcgcatctg
     7861 cgcttcgtat gacatctctt gcatcatctt gccgattcgt atgttttcct aatatatatt
     7921 cgtaaaaaac ttcatctaca ggttccgtat ggagcaagac tgtctgttct ttttggaaag
     7981 aagcaaattg ctcctgaacg tgttctgaca gacctgcatc tccgatataa acaatatccg
     8041 tgttacttgc taatagatca agagcacctg ccagaggatg gatccctcga tgttcttcca
     8101 aagttggtaa tccttgatac agtggacctc tcaactctgt atctcctggt acaaagcctt
     8161 gtacaaggaa tccttgagtt ttgagccatt gatttttttc ctgataccat tctttgtcca
     8221 accctgtttc aagacgtgga taataattat gccaagcttc aatctgtgag aaatcagccg
     8281 ctgcttcttt caactcatcg atatcattag gcgtgatcgt gctggcattc aaactgattt
     8341 tcaattgatg tgaccagtcg gcgatctgct gattagaaat atgataatcc attcttaacc
     8401 ctgttacacc aattgctttc aagggtgtta gatcttctaa tgaaaagcca gctcgtttca
     8461 atgcctctcc tgaaatatcg accatcaact ctagtttatt ctttttcgct tcgcttccta
     8521 gttcgatcaa tcgattttga taagccgaaa catcatcttc cggtatatgc atggaagtga
     8581 aaataccgac aaagccattg gatgccattt ggcggatata ctgtttcttc tcttctgaca
     8641 aagaatcatt cataaatatt gaaaagccaa acatattatc cctctttttc tgtatccgtc
     8701 ttcatttcta tgactagagt atatatctaa agaaacaaaa tttcaatact tttgttaaaa
     8761 taattaaatt ttatttctaa aaattggatt tataatgcca aaagaattga cacagccaca
     8821 aaaaaggcac tgctaagcaa aagcagtgcc ttgaaacatt ttatttaaat aacacaaaag
     8881 tctaattact cataagaaat taattctgta ttgtctacca tgaatgtaaa gaacccttca
     8941 ttaggcattg gatgaaaata agcattgatc actcttatca aggcattatg acagactaac
     9001 aagtagacat tttcttcgtc ttccatgcat tctttcaata aaggaacgat ccgtgcgtac
     9061 acatctaaga ccgactctcc atttgggaaa cgtacagcaa acgctaaacg cgctttttga
     9121 aagttttcat ctttgcttgg cagtccgtca taatccccaa aatccatttc taccaatcgc
     9181 tcatcagcgg tcatcggcaa attgagacga tcaactactg cttgtgctgt atctcttgct
     9241 cgttgaagag gggaatggat gattttcgtg attggaacag gcaaatctac taccttttct
     9301 gctagtgcct gtgcctgcat ctgtcctttt tcagtcagcg gcacatctgc tcgcccgcaa
     9361 actaatcctt gtgcgttcca agttgtttct ccgtgtctcg ttacataaag catgtgattc
     9421 tctccttcgt tgacaaagtt ctccttaacc ataccttgtt gtctttgaga ttacaaggtt
     9481 tagattttct caagagaaat aatctcagta gatactttat ctagaaaggt ttgatcatgt
     9541 tccactagta acatagcagg tttcacgttc aaaatcagtt gttctaattg ttcttgattg
     9601 aagacatcca aataattcaa tggttcatcc catatataga gctcagctgg ttgggataaa
     9661 gatttagcca attccacttt tttccgttgt cccatactca tctgttcgat tttattatga
     9721 aaaacatctc tttccatccc aagctttcgg agattattca aaaatgcttg atagtctact
     9781 cggtttttct tcgcgaattc cgccaatgtc cctcgattgt cttcataatt ttggcgtacg
     9841 taactaatgg tcaaatgttt gggctgggat ttttctccta caaccttgcc gttgaatgtc
     9901 cccagaagat aatggataat ggatgacttt cccgcgccgt taggacctga aattgccatc
     9961 cgctgatgag gttcgatcgt aaaatgaact ggctcgaata acagattttc ataccctaat
    10021 tgaagatttt ctacgcttaa cagccgttta tgatgagaca cttggctatt catcgtcaaa
    10081 gcatcgatat actcgatatc ttttaataat ttttctttct cactgatctg ggtttccatc
    10141 cgattcacga ttgctttaga tcgtttcatc gtccgtgcag catcagcgcc tactgctcct
    10201 ttattcactc gcctagattc cgtatcgatg aatccgactt ttttctttgt tttgtcccct
    10261 tctcgggaac gagaccattc tgctttttca gctgctgttt tctttagcct gctgacttct
    10321 tttttcaatt tttcattttt agctatttca aattcatcac gaagtttttt ctgttcttca
    10381 tagatagaga aattcccttg ataaagttcc agttggctct tttcgattgc taaaacatgg
    10441 tccactactt catcgataaa tccccgatca tgactcacca cgatgaatcc ttgttttttc
    10501 tttttcaagt aagccgctac ttgttttctg ccagagatat ccaaatgatt cgttggttca
    10561 tcgattaacg ggaaatgagt gtcatccaca aataagagtg ccagtaggac ttttgtcttc
    10621 tctcctcctg atagtgtgct gaattctctc cataagattt caggatctgt ttgcatcagt
    10681 tggagctctc tttcaatttc ccatatctca aaatccataa tctcatttaa cacataatag
    10741 gtcaaacgct ctttatcttt tgtttgctgc gggaaataga caaacgcttg ctgatggatc
    10801 acttgccctt gataagggag tttgttttgc aggatattca gtaaagttgt ctttcctcga
    10861 ccgtttcgtc cgataagtcc tagtttccat tgtgtgtcaa agtttagatt ggcttgttca
    10921 aataataatg tgccttggct gtcatagccg aatgtcagat tttttatttc gattttagac
    10981 attattccat cacctcgtaa aaaaagacca gtcctatttt cagaactggc taaagaggat
    11041 gcaagggttg gggcacacaa aaagacccat acatcgaaag aaccaagcca atcccgaaaa
    11101 tctgcacact gctcccttgt ctccacaagg tacagtctga atgcagcttt catcaatcga
    11161 cttaattttt caatgcacgg atccctcact ttgtcctata tttgaaatta cgatagcata
    11221 aagaaattgt ttttgtcaac tacttcggga gaatgttgaa aaatctgctg agtgtctgtc
    11281 ctaagatttc tgttttttct aatgtttgcc tgtgttccat tgcttcctct aaatcgattg
    11341 gtcctaattg aatgcttaag acaatgggga gaaaatcaaa gagtccttcc agctctcggt
    11401 ctctacttcc gcaaatagcg attattggtt ttcgatattt ttttgcaagc actgctacgc
    11461 catatggaac ttttccgtgg attgtttgct gatcaatccg cccttctcct gtaatgatga
    11521 catccgcttg ttcgatctgt ttttctaatc cagtgatttg gctgaccaac tcaaaaccag
    11581 agaccatctt cgctcctaac aaaaaaaggg ctccgccaat accgcctgct gctccgcttc
    11641 ctgggatgtc atctaaatcc acctcccaaa catcttttat ctgctgagca atcttttcag
    11701 cttgtttatc caagaaagct aacgtcgtat ccgttcctcc tttttgtctt ccaaattgcc
    11761 aagcagctcc ttgttgacct gtatatgggt tggtcacgtc tgcagcgatg atcactttta
    11821 ctttagaaac aagaacgttc cttcgatcca aatgaagttt tctagtagat aacaaaggat
    11881 ttccttctgt gtacccttct aaagaagcac ccaaagattg gagtaatcct agaccaccat
    11941 cgctacatcc gcttcctcct aaagtgacaa tgatttgttc cacttcttga agcagtgtat
    12001 ctttgataag ctctcccaag gcataactag aagcaagtcg gatggtttga tccgttggtt
    12061 tatcaagtaa atctaatcct aatacttgtg cagattcaat gactgcggta ttttttcctt
    12121 gccagttcgt caataaataa ctgatttttc gctgacgaaa taagagatcc atcccatcaa
    12181 tcgtacgcca ggttcccccg agcgcgtaat agattgcttc tgcagtattt tctccgccat
    12241 ctgcaatcgg cacattgatt tttcttcctt tacggtcttc ccaaccattt agaactgcct
    12301 gatttaattc aaatgaagaa gcacttcctt taaatgaatc gattgccgcc aaaatatcca
    12361 tctatatccc tcactttctt cttaagtaag acactttttt tcactttttt caaacaaaac
    12421 aatgctattt tcttctgttt ttctatatac taaaagggaa ctcattgaat aaaggagaga
    12481 aaaaacatga aaatcgcaat agctggagcc ggagcaatgg gcagccgaat cggattaatg
    12541 ttgcatcaaa gcggaaatga ggtcgtcttg atcgatcgct ggcctgctca cattgaagcc
    12601 attcgtacaa atggattgat tgctgacttt aatggcaaag aagtagttgc aaaactgcca
    12661 atctattcac cagaagaaat catcgaaagc aacaaacacg tggatttgat cgtagccttg
    12721 acaaaagcga atcaattaga tgatatgttt cgttcgatcc aatccattat tactgagaat
    12781 acttatgttt tgtgtttact gaatggatta ggtcatgaag atgtactgga aaaatatgta
    12841 ccaaagaaaa atatcttatt tgggatcacg atgtggacag ctggtctagc tggtcccgga
    12901 aaagtcacat tattaggtga tggagaaatc gaactagaga acttggaacc agaaggcgaa
    12961 gcatttacaa aaaaagttgt agaagtcttt caagaagcaa aattgaatcc aatctatagt
    13021 cataatgttc gttattccat ttggcgaaaa gcttgtgtaa acggaacatt aaatggatta
    13081 tgcacgatat tggattgtaa tattgctgaa ttaggtgcgc aaaaagcggc agagggcatg
    13141 gttcgaacga ttgtatctga gtttgcttct atcgcagcaa aagaaggaat cacattagat
    13201 caagaagaag tgtaccaaca tattgcttct acttatgatc ctgacaacat cgggttgcat
    13261 tacccatcta tgtaccaaga cctgatcaaa aaccatcgtt tgactgaaat cgactacatc
    13321 aatggtgccg tttggcgaaa aggacaaaaa tatgatattg ctacaccata ttgtgccttt
    13381 ttgacccaat tagtccatgc aaaagaagga atccttggcg cagaataaaa ggctctctga
    13441 tgagaatcag ctggatcagc agctggttct cattttttta cccccatgaa agttcaaaaa
    13501 gaaaaacaca attctcatgt tgccataaga attgcgcttg tttattttca tctatgattg
    13561 gttggcactt ttatttattt ttaacagagg aaagatcgta ttcatattgt tcataaatag
    13621 cggagtcagt agtgaaaaaa cgttttgccc aattttctgc atcaaaaaat tccaacagtt
    13681 ctgatgggat tggttgaaat ggcgtatgca aaaattccgg taattcatct aaatcacgtt
    13741 cttgaagcaa aaaaatattt tcagaatgat aaaactgata atgagctatg gcttgattat
    13801 ctgtcactag tttcttttga aaatacatag attcaaacgt tcgcaaagtc actccttgct
    13861 gcccttcccg caacagttct agaatccccc tcgattcccc ggtttttttc agatattctt
    13921 cataagtaag gtatccaggt tgttcatttc ctcgaagcgg aagaataaag taattggtcg
    13981 taacacctct ttttgtaaat tcctgcttat atttttctgc ccgttctttt ctgccgttat
    14041 ttgttgcacc aaaaaataga tccgtctttg gttcgctaga agaaaaatcc atatgtttcg
    14101 aataaaagga actattatgt tttagtccaa gttctttcgc ttctaaaaaa tcaaaatgat
    14161 aaatctcatc gattgccggt tcatcttgtg cctgcttcag catcgcctcg tgttctgctt
    14221 taaagtggtt ccaaaaaaat aacacgattt ttgcattggt aaatggacgc acgcgtcgag
    14281 ccatctccat atcaaatcca taatctaaaa aaacgatcag ctgataattt gccaactgtt
    14341 gtcgagctga tttaatgaat tccgactgta gtaaatcttt tccggaccaa acatctaaag
    14401 agcggatgga aggaaaaaaa taaagtgatt tatttttcgc aattagcaat acgtcgtttt
    14461 tgttcatctg ttccgcctca ctttctctgt cactttttcc tatgatagcc tgtttaaata
    14521 gtaaaaagcc agtaaaacgc ttcctattat ttttataaaa atttaacatt tctatgaaaa
    14581 tacgatgaaa taaatcattc ttccattaaa atatgcccaa aagttttctg aatagttgta
    14641 attatctgaa aatttgttat cattagtcat tattattatt actatttatt cttactattt
    14701 attcttacta tgcattttga ttgtgaattt aaaagaaaga ggttatttat ggaagataaa
    14761 aaattgaaaa aacaacttag ctcccgacat atcaccatgc tagctttagg tggagcgatt
    14821 ggtgcaggtc tatttaaagg aagtggcgaa gcgattggaa tcgccggccc ttctgtattg
    14881 atcgcttttt tgattggcgg cataatttta tatatcgtga tgaaaggtct gggaaaaatt
    14941 gttttaagtg gtggtgatac gcaccacgga ttatctggat tgattcgtcc atatctaggt
    15001 gctcatgctg ccgattttac cgattgggtc tactggtcca tgtggattat caatatcatt
    15061 gctgaagctg ttgcagctgc ttccttttta cagttgtggt tcccccacat cccagcttgg
    15121 gtatttgttt ttatgttggc tgttttaact actatcatca atctttattc ggtacgatta
    15181 ttcgcagaga cagaatattg gctggccttt gctaaaatca gtgtcattat cctattgatc
    15241 atttttgctg cttatcttgt cggtcagcaa atgttgggta ctggtgtttt ccctaccctt
    15301 caacaattaa ctgatcatgg tggttttact ccccacggta tgaagggaat catcagttct
    15361 ttgcttgtag ttatctattc ttacggcggt tctgagttga ttgctatcac agtaagcgaa
    15421 gcagatgatc ctaaacgtgc gattcctaaa gcaatcaaag gcgtcatggg aagaatcatt
    15481 tccttttaca ttattccttt atttttgctt ttgattattt ttccttggaa tactttagca
    15541 ggcacgaacg ttagtccttt tgtgatggta tttgaaaaaa tgaatattcc atttgcggca
    15601 gatatcgtca atttcgtgat tgttcttgct ctgttctcgt ctatcaattc aggtgtatat
    15661 gcttcttcga gaattttgtt cttccgcttg aaagatcgga aaggttcctc tagaaaacta
    15721 gctgttttaa acaagcatca agtaccgcaa cgtgctgtgc tgttttgtac cagtgttctt
    15781 tatctaggtg tggctctttc ttattttgtc ggtgacaaat tatttggcta tctggcaggt
    15841 tctctttcct atacagtatt attgatctgg atcattatca gtgctgcgtc ctttgtactg
    15901 acactaagga aaggaacgtt atgggacaaa ggaatcagct tttttgcatt agcagtctta
    15961 tggttgattt tccttggtat cctattcacg aactccattg gggtaactgt attgaccggt
    16021 ttgctttacc tgttcattta tttcagttat caaaaaaaga atgatgcttt cgtcttgacg
    16081 aatgaatgaa accaatagag aattttattc tatttatatt gaatatctgg agctattgaa
    16141 aaaaaggagc tgcgacaaaa attgttgcag ttcttttttc cgaataaacg gtgttcaaaa
    16201 accatctgtg cagattaatt gattgttctc cacgctcatg acatgataga agaaaggaat
    16261 gaagaaaaag gagtttgttt taaatgaaaa agtattggaa gtttttgttg atgattggtg
    16321 tttcgacggt agtcatgttt ggtatgatgt atttgaatgt ttatgcgctt gatcatatct
    16381 tcttcagtcg cacacgtgta ttcatggcgt tgatgatggg tgcagtcatg gcagtcatca
    16441 tgttgttgtt catgtggaaa atgtatgaaa acaaaaagat aaatgctatg attttagccg
    16501 ctagtcttct tgtttttgcc ggttccttat ttatggtacg aagccaaaca actgtcaacg
    16561 atacggcgtg gatgaaggcg atgatccctc atcactctat tgctatcttg acaagtaaac
    16621 gagctgattt aaaagatcct gaagtaaaaa aattagcgga agaaatcatt caggcacaag
    16681 aagaagaaat cgacaaaatg aaaaaaatga tcaaagaaat ggaatgacca ttatccatcc
    16741 aaataaaaaa agaggatgtt ccagaagtga tcaacttcaa gaaataagaa gaagctactt
    16801 ctggaacacc gtgaatcaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
    16861 ccaaacaaaa atagcttctc atatttttcg tttggttagg cttattaccg caaatgcaag
    16921 cttttgaaca ccgtttgttc gtaaaaggga gtgtgacaaa agtcttgtca cactcccttt
    16981 ttacgttttt attgttctaa ttgcgctgtc ttgcttactg cttcttgcgg tttcacaaat
    17041 ttctttgaga tcccccaacc tgtaaaggca ctgaccatcg aaaacacagg tgaaagcaaa
    17101 ctgaaaaaca cgaatggcaa gtaagtgagt gtaggaatgc caaatgtact tgctacgaaa
    17161 cttccggcga ttccccaagg cactaagtag ttgattactg ttccaccatc ttctagtgtt
    17221 ctccctaata cagttggatc aaatcctgct ttttcgtaag tttctttaaa tgcattacca
    17281 ggcaggataa cagatagaaa ttgttctcca ataaagatat ttactccgat gcttgtcatc
    17341 aatgttgtaa aaatcaattt tggggtgttc gtcatctttc ttgatactcg ttccataacg
    17401 gttgagatca acccaaattc gatcaacaaa ccacctaatg acaatgttag aataatcaat
    17461 gacacagttg gcatcatgct ttcgattcct ccacggctaa gtagtgaatc gatttgttgg
    17521 tttccagtat ttgaaacaaa tcccgattcg atgacccctg ccaacttcgt taaggaagtt
    17581 tgtgggtttt gaataaagat cattcctact gctacagcaa tattcaacaa aatcgtcaaa
    17641 attgccggta cctttttcca tgcacacaag agcattaaaa atagaggaat cagtgaccag
    17701 atcgaaatag taaagtttgc ttctaaaata tcagtcactt cacgaataga tgaaagactt
    17761 gcggctttac ttgtgtgtcc taaaatcatg aataaaaaga aagaaacaac aaatgccgga
    17821 acagttgacc acatcatatg cttgatgtgt ttgaacaaat cggcatcaac aatagctgct
    17881 gctaaatttg ttgattcgga taatggagac atcttgtctc caaaaacggc acctgaaaca
    17941 atggctccaa caacaagggc tggatttagt cctagtgttg tcccaatacc gaaaaatgca
    18001 atcccaatcg ttgacataac agtaaaggca ctccctacag cgctacctac gattgcacaa
    18061 acaacaaata cagaagggac gaaccactgg acactaatca atttaaatcc aatgaccatg
    18121 agggttggaa taattcctgc ttgtatccat acagcaatca atgaaccgac taaaataaaa
    18181 ataaagattg gaatgatccc cggtttgatg ccattgatga tcccttcgtg cagccaatct
    18241 gtcgggtatt tttttatcac tgcgtacaac atgaccattc cgatagctgc caaaatcgtg
    18301 atattaggtg aaacctttaa tccgacaacg ccaatcgcaa tgcctaaaac aatgaccatc
    18361 aatacaagtg ttgcttctaa aaaacttggt gttccttttt tttccatgat aatctctcct
    18421 tttttagttg ttcttatgtt ctaaaaaagt taagatatcg gattactccg ccacaattca
    18481 tccgcgattc ctcctttata aaaataaaaa gacctgcttt caactaagga cgaatgactc
    18541 cgcggtacca ccttaattaa aaacagatct gtttttcact ttcaaacgat tattcaaatg
    18601 tatggacttt ccatgtaatt cgtcaattag gcttgatcgg ttcacagcga ccaccgactt
    18661 tctgaacaaa acacctaacc actactgcgt gaaaagttga cttgtatgaa gtttaccttc
    18721 cataagatca tttgtcaatg aaaaagaaaa tattttttca tttttctgaa aattattttt
    18781 ataactttcg cgcacaactt tatcttttta ctagaaagta gcgccctctt gtttttttct
    18841 tgctactatt tcttgatgtt cttgtttgat tttttctaat agcttgggtt ctctcaataa
    18901 atctagagct gtattggcaa gtaattccgc tgcaatcgca atagaagcaa gtcctttttc
    18961 acttttagca gcagctttga attcttctgt atgtccggca atatatgtct cagagattgc
    19021 tacagtaggc tgtatcgttg gaataacttg gctaacattt cctacatcag aagaacctag
    19081 acttgttctt tgttccgttt ctatttcttc atcaggaacc cctgcttctt ttgcatgttg
    19141 gaagaataag tcatcgaaag aaggcgttac gatgacatca tcgactgcat tttggaacaa
    19201 tccaaactca aaagtcgtac ccgtgcttaa tgcagcacct cgtacgatat tttccacttt
    19261 ttcatatact ttgtctaatg tttgtcgatt tcctgcacgt aaatagaaac gtccagcagc
    19321 gtagtctggt atgacattgg ccgccacgcc tccatctgta atgattccat ggatattgac
    19381 atcttttggc aattgcaagc gtagagcgtt gattccgtta aacacttgga tcaaggcttc
    19441 caaagcattt actccttttt caggtgctgc tgctgcatga gaagcgacac cgtaaaattt
    19501 gatatcgact ggatcatttg ctaaagattc tgtcgttttc ccataacgaa aagctggatg
    19561 gacacataat gctgcatcga catcctcaaa aaaaccttcc cgaacgaagc ttcccttagc
    19621 tgatccattt tcgcctcctt cttcccccgg agtaccatac acacgaattt ctccgccaat
    19681 ctcatcaatg aattgtttca ctatgctggc tgctaatacg gagtaagtac caaataaatt
    19741 atgtccgcat gcatgaccaa tacctggtaa agcatcaaat tctgccaaaa aggcaattgt
    19801 cggacctggt ttattggaaa tataacgtgc atcaaagcct gttcggtgcc cagctacttc
    19861 tttttctacc cagaaccctt ctttttccaa ttgtttgatt aaagctgctg aggaataaaa
    19921 ttcataatta cttacttcag gatggtcgtg gatagctagt gcaagtgctt gatattctgg
    19981 taaacggtat tgaatcagat ccttcaattg atttgttgag agtgtggttg tcataaaaat
    20041 ttcctcctat gctagtcgtt ttttcttatt ttctcccgaa tgtttcccac gcaggtacgc
    20101 ttgaaccttt tgatgttttt tcgattactt tggctgtttc ttctgtttga taggcatcta
    20161 ctattttttg atacgtttta ttgtcttcat cttcttttct tgcaacgatg atattgacat
    20221 aaggacgtgc atgctcatcg actggttcta agaagattgc atcagaagcg ggattaaatc
    20281 cagcatctac agctacaccg ctattgatga ctgaaatatc tacatctgac aaagcacgtg
    20341 ctgtttggga agcatctaac tcagaaatgt ctagattttt tttattctcg gtaatatcag
    20401 aaacggtagg cgcttggcct tttgccttgt ccactttgat caatccagca gtttgcaata
    20461 acagaagagc tctgccgcca ttcgtgacat cattagggat tgcaactttt gctccatctt
    20521 tgatatcagc cacatctttt aacgtattgg aataaattcc caacggtgca ttaaccgtat
    20581 tcccaatcga tactaaatca gttccgtgtt cttcattgta attgtctaaa aagaattgat
    20641 gttggaagga attcaaatcg atcgatccat ctgctaaagc cgcatttggc tgattgtaat
    20701 ctgtaaattt cacatattcc aaatggatgt tttccttttt caaacggtct ttcacatcgt
    20761 cccatacatc tgtatcttcc ccaatgatcc ctagttttac ggtcgtttct tttttttcag
    20821 ttgtagctgc ttttccttgg ctgcatcctg ccaaccaaat aattgctaca aatgtaatga
    20881 ctgcaaaacg aataattttt ttcatttttt ctcctccatt ttcaatgtgt gactttttta
    20941 atcagttgat tactgataaa ttgactcaaa aaaactaaga ccagaatcaa taatgtagcg
    21001 acaatcgtta catctgtttg gaatcgatta tatcctctcg atatagcaag attccctaat
    21061 cctccggcac ctactgctcc agccatggta gtcagtccga tcacattgat gatcgtcaac
    21121 gctgagaccc gaatgatacc aggtaaccct tctaacaaat aaacacgtaa aatgatgccg
    21181 attgggctcg tgcccatcgc ttctgctgct tcgatcaccc caggatcaac ttctagcagt
    21241 gcattctcga tttgacgggc aaaaaacggg acaacgccaa aaatcaaagg gactaaagct
    21301 gctttggcac caatcgtcgt tccaactaag aaacgagtag tcaaagctaa aagagaaagc
    21361 aagataatga aagggatgga acgaatgata ttgatcaact tatcaagtat ttcgaacagc
    21421 agcctgttag ctagtaatcc atttggacgt gtcactacta acgtcacacc taagatgatt
    21481 ccgatgatcc caccaatgaa tgccgtccaa aaagacatat atagtgtttg ctttgtcgct
    21541 tcgatgaatt catccggaat caaatagaca ttcggcaaat atgttttaaa aaattcagtc
    21601 attttagttt cctccttcga tgactttgat acggttatct aattcatttt tttgtaaaat
    21661 agttactttt acaccagatt taatcaaata atccaatgct ttttgccgct gtttggtttc
    21721 tccagctaaa gtaacgatca aacttcctaa gggtgtttct tgtaatagct ctacattacc
    21781 gtataagata ttcgctgtta cttggtactt gctgtaaaga tgagcgatta gaggttcgtt
    21841 cgtctggttg ccgatataac ttaattccac caaccattta tttgcaatct gatcggcaaa
    21901 agcagcatgg gcactgattg tttccaatgc ttggtcaagg tgtgtcgctg tccgaataaa
    21961 atctttagtc aattcttctt caggatgact gaaaatctgg atgctgctac ctttttcaat
    22021 gattttcccc gcttccataa ccgctacctt gttgcagatt tctttgacaa cttgcatctc
    22081 atgagtgatc aggacgattg ttagacctag ctgacgattt agttttttta acagtgcaag
    22141 gatctgaagg gtcgttttcg gatcaagtgc actagttgct tcatcgcata acaacacttt
    22201 aggttcattt gccagagctc gtgcgattgc cactcgttgt ttttgccccc ccgacagttg
    22261 tttaggatac gcagatgctt tttcttccag accgacaagt tccagtagtt cattcacttt
    22321 ttgacgacgt tcctgttttg attttccaga atactttaga gaaaaatcta cgttgtcaaa
    22381 aatcgttcga ctttccatta gattgaaatg ctgaaagatc ataccgatgg cctttctttc
    22441 ttttcgcaat tcttttggag agagctggct taaattcgtc tgattgacga tgacttctcc
    22501 tgctgttggt ttctgcaaca aattcatcac gcggactaac gtactctttc ctgcacctga
    22561 gtaaccgacg atcccataaa catccccttt ttcaatcgtt aaatctacag aatctactgc
    22621 ctgtatctct ttttcttttt gtttgaaaca tacagaaata tctcgcaatt caatcattaa
    22681 cattcctcct tgtctataaa aaaagcatcc tcaagaaaat gaaaatagtc attttcttga
    22741 ggacgccttt ttgcgtgtta ccacctcatg ttcaagctgt gttcacacac aactcctctt
    22801 caagtaccat catactctag caagatatcg gtcgcagccg tcaccgcctt acatctatgc
    22861 tcgggatgaa ttgctcgatg gccattttcc aatagtcgtt ccttgcttct tctcagctac
    22921 cgaagctctc tgtcaaaggt tcttctatcg tacttaccat ttcaatgcaa ataatattca
    22981 aaaaaaaaat ccttttacta acataaacta tgtcagtaaa aggacgacgt atcgtggtac
    23041 cacctttttt cggaagtttc cttccctcaa gcccaacatc tcaaaaagag agattggcgt
    23101 tcgttttaac ggtcgaacat ttccgaattt gcctacttat cgacaaatta ttctccaagg
    23161 ccattttcag cttttcttac ttgactcttc acaccagcca gagcctctct gcaaagtttc
    23221 caagcttact ttccttctca acgaaatcaa tattttgttg ttgggaataa tctacaggag
    23281 gaaaactgaa aagtcaagca tgaaatccaa attatttttt ctatatttat ttttctgttt
    23341 ttttatctaa catttcaata acagttcgaa gatcttttcc ttccgcaaga aggttcaatt
    23401 tttccttctc ttcgacgcta taatttcgaa caaaactgga atcgggaatc aaaacaacgc
    23461 ctagtcctgc acgtgaagcg gctgtcgctc ctactaaaga atcttccact gcaaaagctg
    23521 aaagagcttc taatcctgtt cgttccaaag ctgtcagata agggactggt gaaggcttgt
    23581 tctccgatac ttcatctccg aacgtaaacg tatcaaagta atcggctaat tgaaagtgat
    23641 ccaagatatc tgttgctctt ttcgtaacag ttgtcgtagc aatccctagt ttcatatgac
    23701 gttcttttaa gtctgtcaat atttcttttg catagggttt caattggata gctccacttg
    23761 tcagctgttg ataaaaatat tcttcacgct ttgctcggac ttcctgcact ttttcaggac
    23821 tgcctgctgc ctgcactaaa atatcaaaac tttgtttcca tttttttccg ccccaagcgt
    23881 caacgatttc tttcgggata tgaatatcaa attgctggaa tccccaaagc caaccatccc
    23941 gatacgttct ttccgtatcc actaacaaac catctaaatc aaatattgca cctttcatct
    24001 atacattaat cctttccaaa acaatacacc tagtttagca tgactgatcc aaaatgaaca
    24061 gaagcataga gcccagccgc atcacttata agagttcttg gaatcgacgg atatagattt
    24121 tcttcacagc tgtcacaagc gccagataag cgacgactgt tataaaaaac cataaccagt
    24181 aattgccagg gagcggcatc aagcctaact cagcaccaaa tcctgtgaat ggaaggactg
    24241 aaccgacaat gattcctaat gtagtgataa ccagcatggc aaatgttgca ttgctttgga
    24301 taaatggtat tttcggtgta cgcaatgcat ggagtactaa tgtttgtgtc cacagggatt
    24361 cgacaaacca tcctgcatgg aataatgcga taaaaatcac ttgttgtgag ctatccaatg
    24421 tatggaaact tccaccaact acagcaggac aaataacaaa atacatcaac aagtaagtag
    24481 tgatatcaaa aatcgaactt gttggaccaa aataaaccat gaattttcca atactagatg
    24541 cattccattt ctttggctca tccaaatatt ctttatccat ccgatcccat ggtaaggaaa
    24601 tgcaagaaac gtcatagatc agattcaaaa atagaagttg caaaggcaac attggtaaaa
    24661 atggtaagaa cgtactggca atcagtacgg aaaacatatt gccaaaatta gaacttgctg
    24721 ttgccttgat atatttcatg atgtttccaa aagtttctct tccagaaaga aggcctcttt
    24781 ccaaaatcgt caaatctttt tctaaaagga tcacgtctgc tgattctttt gcgatatcca
    24841 cagctgtatc cacggaaata ccgatatcag ccactttcat tgctggagca tcattgatac
    24901 catctcctaa aaatccgaca gtatgcccat ttttacgtaa agctgtcgtc aatcgtgctt
    24961 tttgctgagg attcaatttt acgaatactt cgtgttcttc agctacttgc gctaattcat
    25021 gatcattcat ttccgagatt ttctcaccag taatcaattc attgatttct aggcctactt
    25081 gacggcacac agaccgtgtg actaattcat tgtccccagt cagaactttt acagctacac
    25141 catgttcttt taaagctttc aacgcttgtt ttgttgtttc ttttggtgga tcaaggaaag
    25201 caagataacc aatcagaacc atgtctgact catctttgac tgaaaattct ccaacaacgc
    25261 ttggattggt cttttgtgcg acaccaatca ctcgtaagcc gtcttcatta agtgattgta
    25321 cttttgcaag caccgcttct tttcgttctt ttgttaatgg tgcgacagta ccaccaagat
    25381 caataaattt agaaatgttc aacatttctt caatcgcgcc ttttgtgatc atctgtgttt
    25441 tgccagccgg atcagcgact acaacactca tacggcgacg ttcaaaatca aaaggaattt
    25501 catctacttt ttgataattg attgtatctg tatctaaagt ttctgctgct tcatcaatga
    25561 tagccacatc taacaaattt ttcagccctg tttgatagta gctgtttaga taagcatggc
    25621 gcaaaacacg atcatcttca tttccttcac aatccatatg atattcaaga atgattttgt
    25681 cttgtgtcaa tgttcccgtt ttatccgtac ataacacatc gatcgcacca aaattctgga
    25741 tagcatttaa atttttgatg actgttcctt ttttggccat cacacttgct cctttgacca
    25801 aattcgtcgt aacgatcatt ggaagcattt caggcgttaa accaactgcc acagataatc
    25861 caaaaagaaa agcttctaac caatctcctt ttgtaagccc attgataacg ataactgctg
    25921 gagccatcaa tgacataaat ttgatcagca gcatggatgt tttgtggatg cctaattcaa
    25981 aacttgattg gatgggtttt tcagatagcg tttctgctac atgcccaaat agtgtgtcat
    26041 ttccgacaga aacaatgatt ccttccgcgc ttccactgat gacattgctt cccataaaga
    26101 caagattttc ataacttgtt tcactttgct gatcgtcttc acatactttc gcatgctttt
    26161 ctactggata gctttcccct gtcaatgctg cttgagaaat gaatagatcc tttgcggaca
    26221 ataaacgcat atccgctggt atcatatctc ctgctgaaag ccgaacaaga tctccacaga
    26281 cgatttcttc cataggaatc tctgtatact cgccatttcg tttgacagct gccgttactt
    26341 tcacaagtga tttcagtttt tcagctgctt gattagaacg cacagactgg atcaacgtca
    26401 tcgtcccact gacgataacc atagtgaaga taatggctac accaaagaga tctttatctc
    26461 ctggagcggc taatacgtat tccgtaatga atgaaataat tcctaatgca atcaacacta
    26521 atgtaaaggg tgtgatgtat gctttcaata tttctataat gaatggtgtt tttttgccat
    26581 atgaaatcgc attttcacca taagtttctc ttgaacgctc gatttgttct tctgacaatc
    26641 catttggtga tgcatggaac aattcaaata attcttgctt cgtttttcca gcaaaagctg
    26701 catagatttt ttgtgtatct ttcttttttt ccatctcgat ctctcctttg tgtccaaaag
    26761 acagctgttc tcgctgtttt tggtcttttg ctattcatcg gatcgataga gaaaatgctg
    26821 aaaggggctg agaacagata gataatagcc ctggcctttc ctctaaatcc ttgtttttcg
    26881 aatgactgtc atccatttgt tctcatctcc tttactaagt aattttaatt catgacttcc
    26941 aatcattttt cgaatagtta ttttcccaac tcgctcacct ccctttttgt tttttcacct
    27001 atgggatttg cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag
    27061 caaatcaaaa aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa
    27121 gcagatatct gtttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc
    27181 cattggcgtc tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa
    27241 caaatcctat tcggttttcc ctcgttaata taacaactaa aaaagaggat gtcaaatcaa
    27301 ttttccatgt ttttcatact tttttaaaat acacgcgtta tatttcctgt ctcacgcagt
    27361 tttatttgtc tcattttcca cctcccgcta taatagaatt atagtaggaa acgaggtgct
    27421 gttatggaac gaacacttgt tattatcaag ccagacggcg taagacgcca tttggtcgga
    27481 agcatcatcc aacgttttga agcaaaggga ctggccattg ctgaaatgaa atttgcaacg
    27541 atgacacctg aactagcaaa agaacattat cagcacttga ctgaacgtag tttttttgat
    27601 gagcttatcg attacatgac ttctggtccg gtcgtttatc ttgtgctggt cggcgaggaa
    27661 gtgatcgata tcgtccgtaa gatggtcgga gcaacaaaag gggcggatgc cgttccaggt
    27721 acgatccgag gagattatgc actgccgggg actgaaaaca tcatccatgc ttctgattcc
    27781 cgtgatgctg caacaaaaga gatcgcccgc tttttcccgc cattcgatac aaacaaaccg
    27841 tctgaaaaag cggcgaagtg atacaaatcc taaactgttg gtctaactgc tgggtttagc
    27901 aatggccagc agttttttct ctaaagaatt cctttgattc ttccctaatg tatattcgat
    27961 cactgcgaaa aactcttggt gattttccga tttttcttcc cctaaagcag cagaaatcag
    28021 tcgttggtaa taatccgcca tcaatgtttc ataaatcact gtacttcttt ctttatgcgc
    28081 taaactccgt ttcatatctt cattcacgct acgtgccttt tttaagcgac cattttctat
    28141 ataaaacaaa gtcaagttcg tcaaaaaagt aaagagatac tgctcgtgat tattcagttg
    28201 cgcgatccgt cgcattcttt ttttgatttt caaataatta ttctcaataa aacgatcact
    28261 gaatatccaa ggaaaatctg ctagaataga tacttcaaat tgcccatact ctggtacttg
    28321 catcaaatat tcttcaatga tacgacattc ataacgcaaa aattcaaatt ccgtctcaga
    28381 tacttctgtt tttttggaca tgtgcagatt caaaaccaaa tagtacagat cttctgtttt
    28441 cttatagtgc ctgcggttat tagcaatttc tttgtaagga tcaaagctgt cttcttgaat
    28501 ccgatctagc attcgtcttt ttttctttaa tttttttggc tcatcaagct gatttcgtat
    28561 ccattcatat tcggcaagtg tcatgttcat tcgctctaaa aatgatttca atgttccaaa
    28621 cgatactttt ttcactgaat ttgtctcgat cccgactagc gaagttttag aacagattcc
    28681 ttccgctaat tgggtctgtg tcagcttttt gttgactcga agcttcttaa tcaattctcc
    28741 atcatacata atagttctcc ttttttaatt gaaattaacc cttggctttt ccagaatgct
    28801 tgccaataag aggaatgatt atttcaattt aaattttccg cattcaataa atagatacgt
    28861 taaaaataaa aaatacacga aaaaaataaa aaaatgtttt tctcataaaa aataccaacc
    28921 ttcaagcatg agactgctgt ctaatccctg aaggttggtt tgattgttta ttgatcattt
    28981 ttttactgct ccgcattggt tcctgttact ggatatagga tagtcgttgt tttattgccg
    29041 ttttttgaaa agacactggt cgaatgttca cctttttgct tttcgattgc ttcattacgt
    29101 tccgcttcgt ttttatagtc atattttttc ggatcaatcg tctcaaatcc gtttggcaca
    29161 taaaaccgca agagattttc ctgattcagc ttatctgaaa gacttaacgc agtcttgact
    29221 ttttcctgat ctttggagat ttcctcgtcg attcctgctg ctttcgtatc gatgatttct
    29281 cctgtctgat tattgtaagc ttttcctcct aggacggtgt atttgggcgt cacgaagttc
    29341 ccatttcgaa aagcaactac ttgatcgtgc tgagaagata ataagtctgt accaaaatga
    29401 agataatctt tatcatcgat tcccaataga tgtagtaatg tcggcaaaac atcgatttct
    29461 ccaccatatt catgattgac tgtcccttta gtgtaaccag gcatatggat catcaatggc
    29521 actcgttgca tctgtgcgtt gtcaaattca tcccatgtat ctggatcttt accaagagca
    29581 cttgctaaat ctttattatc tgtattcgag atcccaaaat gatccccgta aatcacaaag
    29641 attgaatttt ggtaaatacc agattttttc aaatacgtaa aaaattgttc caatgattga
    29701 tccagataat gggccgttcg aacataatta tctactgtgc tattgccagt attcaaagat
    29761 ggaaagtcga aattcttgtc atccgtataa taaggcgtgt ggttggttac ggaaagaaat
    29821 ttcgtgtaaa acggctgttg tagatgttca agatattggg cagattctct gaataaatct
    29881 ttatctaata tcccataacc taacgtttca tcggattcat caaaataaga tcgatcaaag
    29941 aaattgtcat accctaaatt tttatataca tgatctctat tccagaaact agctacattc
    30001 ccatggaaaa cagcacttgt atagccttct gtttgaccaa gggtcgctgg tgctgcttga
    30061 aagacattat ctgacccaag ttctgtgaat aatgagcctt gaggtaaacc aaatgtgcca
    30121 gtttccaaca tgttttcagc atcacttgtc tttccctgtc ctacttcatg aaagaaattg
    30181 tcaaaactga tcgttgcttg attctggaaa atactattta aaaacggcgt gacttcttgt
    30241 ccgtctacct tcatattgat caaaaactgt tgaaaacttt ctaaatgaag gacaatcaca
    30301 ttttttcctt tagcaatccc aaatgtttca ggatttggct ctgcataatg tttctttgta
    30361 tagtctaaaa cttcatcaat tccattactg cttgcatgcg cgcgaacact gctagtcatt
    30421 cctgttttga ttccgtcata aatagtgtac gcatcgattc ctagatattt gacaatatag
    30481 gaacggtcaa atgtccgctg tagcaattgg ggacggttgg cttcgctcaa cgctaaattt
    30541 cctgaaaaaa tcaatcctga aagacaggta acagcgatag ccatcggttt gctgacaggg
    30601 ttgcttttga taggtatttt cttgaccttc aaccaaacca gtagcccaat gaacacagcg
    30661 atatccaacc aatagatgac atcatggggt ttcatcaaag aaaaggagct tcctgacaac
    30721 ccttgtgaca ctttcgaaaa gcctaataca gatttgactg taatgaaatc tgtgaattcc
    30781 cgataaaaaa taatattcaa gtacaacaaa agcgtattta aaatatcaat gatcaaaaga
    30841 atggctaacg ctggctttgg ctttttaata tacaaagaaa ggccaaaaaa caataaggta
    30901 gtcgcaatcg ggttgatcca caagataaag tactggatcg taccttccac acctaatgaa
    30961 aaatctgcat agtaggcaat gatcgtcttc agccagaaaa agaaaacagc tgtcaaaacg
    31021 attgctaaac gattttggca caatttttta agttgtgtca cgtatatcac ttctctctat
    31081 tcacattttt tcttattttg ctcctgaaaa atgaaaattt cctaaaaaag ttgttatctt
    31141 aagatttttt tattctttct aaagattatt ccattcattt ttcacagcat aaaatcgttt
    31201 cggtgtaagc ggttaaaata gtaaggagat aaaaatcttg tggtgaccga tttactctat
    31261 agtattcaaa agtagtttct cggtattcgt acaaaaaaaa catactaaga ttagagggga
    31321 acatcattga cgaatgatgt tctcctctat tttctatttt tagattatag tgaagagtga
    31381 aagaaagttc gaacaaagcc ttgaggacta cgagtcccta tcaaaaaccg aacttcttta
    31441 ctgtcatttt agaatcaggt ttacgtatgt tggggccatg agcccgtgta taggaaattg
    31501 gaatcgaaat gaagcagtaa agttctttca acacttaaaa aggaggaaaa caaatgcctt
    31561 acattcttgc attagatgtt tcaatgggac acagctacag tgtcctatac aaagacgaca
    31621 cctgcttatt tgaagacgag atcgaacaca ctcaaagagg atttcatgca ttactcgaag
    31681 agatccagca cttgcctgaa cgtccattga ttgtttttga agcaacgggt atttattcca
    31741 gaccagtcga aaagttcttt caagatcatc acttttctta ctgtttgtta aacccgttag
    31801 aagcgaaaaa acagatggaa gaaaacacct tacgcagttg gaaaacagat aaatcggatg
    31861 cccatcggct cgcacagact catcttaaaa atcaacgtca accaaaagaa gtacaaagca
    31921 atttttactt agagatgcgc gatttagccc gtttttatca agaaattgag aagaaaatca
    31981 ctcgactgcg catggattta cacaactgcc ttcagttgac ttttccagag cttgaacagt
    32041 tcttttcaaa caggctgacc ccttacgcat tgacattgat ccgtcttttt ccccatccag
    32101 attttgtatt ggcatctact cggacaaaaa taaaaaataa actgatcaac gaaacacggg
    32161 aaaaaatctc cgcgaatcgt gccgagcaaa aggcagatca aatcatccac tatgcacagt
    32221 gtgcctatcc agctgttgaa aaagacagta ttcactgtca aaaaacgatc tattacgccg
    32281 aacttctcca agatttactc gaacaaaaaa aagcactggc gacacaaatg atcaaaaaag
    32341 ccgaagcctc tccttgcttt ttgttgtacc aaacgttccc tgggattggc gcactcacag
    32401 ccgctttatt gctcggtgaa ttaggagata tcacacgatt taaaacccat aaacaattga
    32461 atgcctttgt cggaatcgac atccgacggt atcattcagg taagtatact ggccaagacc
    32521 gcatcaataa gcgtggtaat cccaaagcac ggaaaattat tttcttcacc attcgaaata
    32581 tgattcgcca gcaacgcgca gctcctaatc atattgtcga ttattactac aaattaaaaa
    32641 agcaacctat ccctaagaaa gataaggttg ccacagttgc ttgtatgaac aagcttctga
    32701 aatgtatgca cgctatggtt agggcacata cagagtacga ctatgcgtac gcggtctcag
    32761 tggaccatta actaaaccac ttacagtata ccacttcttt gcccagatcg aaaatttttt
    32821 acgaacgggg ctttatttag tgtgccttga ttttgtcgct ttcgtttacg agctattttt
    32881 cttgcacctt ttacttgact aatcgtagga aagaggctgt gacaaaattc ttgtcgcagc
    32941 tccctatttc ttgatgcagg acagcttttt cacaacctct ccactaaacg gtgttcaatc
    33001 agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggagctatt ttcccgtttt
    33061 tttctctaat actcaggtca aaccgctgat ttcacaacct ctgattcacg gtgttccaga
    33121 agtagcttct tcggaaataa gccgaaattt tacaaaaact tgagaagcac ttttcgtaaa
    33181 tttcttctta tttcttgaag ctgaccactt ctgtcacaac ttctgttttt cattgtccat
    33241 aggtttgata ttgtccttct gatgactcat tcgtaatgat tgaggcttga ttttcttcat
    33301 ttcccgttat cgtttgtcca ctgccagaat ctgaagggct tagttctgtc gactcgtttt
    33361 tttgagaaga agcagttgtt ttcttgactt gttcgttggt ctttttccat tccgctagtc
    33421 gatcggccac cacttttttg tccacttttt cagaataagc tgtaaccact tttccatttt
    33481 tcaaacgtgc taccttgtcg ccatcatgag tcaaaagcaa aacgaccaaa tgtttgtctt
    33541 tgaattgata ttgtctgaga gaaatcgcac taagtacgtc atcgatattg ataaattgat
    33601 tttcttcttt tccttttggc aaatagatgt ccaattcacc tttttcgtaa ttggcagtta
    33661 atttatattc ctcccaccaa taagttgaga attctttttt gaaattatct actgattctt
    33721 tgcttgctac gattttttct gtttctttct gagcaagatc tgctgtcatt tttcctgaaa
    33781 atgctgcgga taaaataagc agtaagaaaa aaccgaataa ccaaaagaga agaaacggtc
    33841 tttttttcct catgccttcc tccactccta tttaattttg actattctat tatatacaaa
    33901 aaatgaaata gaaagacaaa aaagttgaaa aagatcgtaa ataattgaac ttctttttcc
    33961 tgaagcggta agaacgggaa ttttcataca ttctctcttg gctattcttt aagcttcaaa
    34021 tccatttctg tctgatacat ctttgaacca atgacccgat ttcttgatcg ttctttttcc
    34081 atcttgtgct aaatctactg aaataaaacc gtaacgattc ttataggcat tggtccacga
    34141 ccagttatcc atgcatgtcc acatatgata acctaaacaa ttggcacctt cttgaatggc
    34201 ttgatggaca tatttcagat gagattgaac aaattcgatc cgataatcat cgtggatcat
    34261 accatcttcc ttgataaatc gtgcttcatt ttctacaccc atgccgtttt cagaaataaa
    34321 acagtcgata ttcccataat gatctcgtac attgatcaaa atatcataga ttcctttttc
    34381 atagatctcc catccgcgat aaggattcat tttacgacca ggcatttcat aattgtcgaa
    34441 gtaatcctca ggcataggac cgtttttagt atcgattggt gtttcttttg cttttattcg
    34501 gcgtggttgg taataattaa ttcccaaaag atctactgtg ttttctttaa tgatcgtcaa
    34561 atcatctgga tgaatctccg gcattagatc gagttctttt attctctcaa tcaattcact
    34621 gggaaactcg ccttttacag ctggatcaag gaatgaacga ttgaagaacg catctgctat
    34681 tcgtgctgct ttaacatctt cgggatttga ttcgtctctt ggataactcg gtgtcaaatt
    34741 taagatgatt ccgatcacac cgctttgatc catttcatgg taagctttga tggctaatgc
    34801 tgaagccaac gcttcatgat aaccaacttg gattgccttt ttcatatctg attcttccgg
    34861 ataatgccat cgatacaaat agcctgcctc gactggaacg attggttcat tatgggtaaa
    34921 ccattttttt actttcgagc caaacaattg aaaacaactt tttgcgtatt tttcataggc
    34981 ttcaaccgtt tctcggttca accatccgcc tttttcttgc agatacatcg gcatatcgaa
    35041 atgatacaga ttaacaaagg gttcgatacc gttttcaatc aactcatcaa aatagtgatg
    35101 atagaaacga acggcttctt cgttgattgt tccagattca tcaggtatca aacggctcca
    35161 ttggatagag gttcgaaatg aattatgccc agtctgtttc atcaactgta catcctcttg
    35221 atacttcgta taaacttgcg aggttttttc aggtcctact tgatcaaaga acttttgcgg
    35281 ttcgtttttg taccaataat caaaaatatt ctctcctttt ccatcgcctg aaattcttcc
    35341 ttctgtttgt ggaccacttg ctgcagagcc ccaccaaaaa ttttttggaa attgatatct
    35401 tttcatcaaa gcacctcttt ttctttttta gttgattcca tgtatgatat tttccatcac
    35461 aatcactttt tcattttctt cctcttcttt tttgattcgt ttcaacagct gttctgctgc
    35521 tcgttccccg attttatctg ttggtggcgt gatcgtgatt tttggaagga tactcagatc
    35581 gtttttatct aaataatcat agtgactgaa cgcaatatcc ttcccgattc tgacaccttc
    35641 ttgattgaaa cattcgatgg caccaatcga catgttgtaa ttgctgacaa aaatagcagt
    35701 tgcccctttt tctaataatg cttttgcccc gtaatagccg ctttttctag aatagtctcc
    35761 tgtaaagatc tctaaatgga tagacggatc cttttgtacc gcttctttgg ctccagctaa
    35821 acgttctctt gctacgtaat ctgtttgagc ggcagcaata aatcctattg ttttatgtcc
    35881 ttgtccgcca tgatgagcaa tcacttttgc tacactttct cgatcattca ctactattgt
    35941 atcaatacct gctggcccac ccggacaatt caatgcgaca actgggatac tcatttttgt
    36001 cagcatttca aatccagacc actcttcttc tgataagaag acgatcaatc catcgatcgc
    36061 atgcttcatc aggtattcga ttttttgttc gattttttct ggctgatcgg caaacccgct
    36121 taataataag ctatacccat tttcttcagc tactctttcg atccctgaaa caacctccga
    36181 accaaatcgg ttagatatcc cgtttatcaa cagtccaata gagaaacttc gattattctt
    36241 caatccttta gcaatgatgt tttctttata gtccaacgct gaaatcgctt ctgatatttt
    36301 ttccatattc ttttccttca gctgctgacc gtttaaatag cgggaaactg ttcccactga
    36361 aacaccagcc atcttagcca cgtcacggat cgatgccata cattttcact cctgccagtt
    36421 acatttgatt tctttttctt gttgttcgac catgatgacc gctttctttg cataatgccg
    36481 cttgtcatca atatccatat aactaaagtc ttcagaagtg agttcaaaaa caagcagtct
    36541 ttcctcttga gaccgcaatt catctttacg aaaagctttc aataatttcg ttctttggat
    36601 gacaacgccg tcaattaatc ggacatagac aagagtactt gttttacctg aaacacttcc
    36661 tgtattttta actttaattg atattattac tttgtcaata tccgttttga cttccatctg
    36721 actgtattca aatgttgtat agcttagacc atgtccaaaa ggataaaatg gacttccgct
    36781 taaatcataa taattctctt gtttagaagc agcacgctga tagtaataaa caggtagttg
    36841 ccgactattt ctaggataag acaaactcaa tcgtccgctt gggttatttc ttccagaaat
    36901 catctgcgca atggcttttc caccctcctg tccaggaaac catgctgttg caatggcatt
    36961 cgtaaatgac tcgattttca tcaaatcgta gggtcttcct tgcacaagta tcgtgatgat
    37021 tggcttgcct agttgataca attgttcaat cagctgttct tggtatccac ctaaagaaag
    37081 agaagcaaca tctacatttt ctccagaatc catgttgatc cctttcgatg aaactgcccc
    37141 gttacgaaga aattccatat caaagttccg cgcacttgat ccacctagaa ctgcaatgat
    37201 cacatctgag ttttgagcaa gggtcaccgc ttctaataat ttttcttcct gatcctcctg
    37261 atttcttact tcacatcctt gtgcataaga aacctttgat tggggaaact ctgtttttat
    37321 ctcttgtact atcttttttt tcaaacggtc ttctgtttgt ggtgcagagt agtctcccaa
    37381 caagtggtaa acatcgtgag catttggacc tataacagca atgtttttta cttgttgatc
    37441 caacggcaat ataccgtcat tttttagtaa agtgatactt tcttttgctg tctctaaatt
    37501 taagtgctct gattcctcca tgagatgatc ccaatactcc tttggatcgt ttgcaaatgg
    37561 ctgttcaaat aaacctaata aaaatttgac agataaaact cggcgaactg cttgatctag
    37621 tagcttttca tctacgactt gattatcaat ggcttctgca atcttggtat aggtctcatc
    37681 ccacaaactg agatcgattc ctgccgctaa tgcatatgct gcagcagtct ttttatctgt
    37741 aaaaacatcg gataaacgat ccaaagccac accgtctgcc ataacgattc cttgataacc
    37801 gatctctttt cttaatgttt gctcaaaaag cgctcgattg atatgacaag gtatcccgtc
    37861 gatgtcatta taagcagcca tgacacctac tgcatttctt gtactcttca ataatggtcc
    37921 gtaaatatcg gagaactctc tgcgtccaat cgttactgtg cctgaattat gaccgcctaa
    37981 agcttctcct tgtgcgatac agtgctttaa cacaacaccg atctgatcct tcttcttatt
    38041 gatttcctct actgtctgat caagaaaaga aactttttct gaaataatct ctccttggaa
    38101 ccctcgaacg accgcttcag aaaaacgagc tgagagaatc ggatcttccc caaagcactc
    38161 ttctgtcctt ccccaacgag gatcttttgc cagatccaaa gtcgacacca gcgctaaatg
    38221 aacaccttta gcagccagtt cttttgccat caatcgactc gattgttcta ataatgctac
    38281 attgaaagta tttccacggc cgatattcgt tggataagaa atcccatcca agccttgatg
    38341 accatgtgga cattcttcta caagcaaaac tggaattttc catcgcgaat gctgtagtac
    38401 atattcttgg actaggttag ccaccttcca gctttctttt gcaggaatcc cattcttata
    38461 atctgtttta gaccacggat cagagcgaaa cagaccgtat aaggcaccca gaccgcctcc
    38521 ccatttaaca tgttctttta acttttctgt taattcgatc ttgtctccgt ttttttcata
    38581 acattcccaa ccatacaaat gctggtttac ctgtcctact ttttcagcaa tattcatcct
    38641 cgaaagtaaa tcttctactc tttggcggat ggttaacttt gggttttgat acgggtaatc
    38701 gtacacctta atatcctcct tctgccaatt ttgccttatt agcggctaat acaaatggca
    38761 ggaataaaaa tacatctaga atgataagta ttactgttaa gactgctgct cgtatatcgc
    38821 ctcctgtagc aataaaagat tgtataaccg gtggagtgat ccaaggtgca gcagcaactg
    38881 ttttactgat gattccaaga cttgtggctg cgtaggcaat aaccaagtta atagatggta
    38941 taatagcgca tggaatcata tatattggat tcatgactac tggtaatcca aacataattg
    39001 gttccgtaat attaaataaa caagtagtca gaccaaattt agcgattgat cgctgttctt
    39061 gacgtttaga agcccacaag atagcaataa ctaacggtaa aatacacccg ccacctccga
    39121 tcaatccaaa tgcattggta aagggctccg taataatatt aggaatttct tttcctactg
    39181 aaaaagcatc catgttttcc tgaatagact gtaacaaagg tgcttgccgt ataggagcca
    39241 aaatactcga accatgcata ccaaacaccc ataagaaatc agagaaaaac tgtaaaaata
    39301 agatgcctgg gacggataaa acaaaagctt tcaatggtgt ctgaatcgct aattcaatta
    39361 attgcggaac attcaaacca gtcgtatggt ttaaagtaaa aacgacaact gaaaaaaata
    39421 aaatgacaac tatttctggt actaaagaat taaatgacgc agctactgcc ggtggaactc
    39481 cttctggcat cctgatttta aaactcttca ttttcgctaa tttgataaat aattctgtac
    39541 tgacaagggc agcaatcatc gccgttgcta agccagacga agaagtcaga ctttgtaaga
    39601 aaacccctgt agcttctgct gtatttccgt ccatcaaagt gactgtactt gataaagaca
    39661 tcaaaataaa cattacggct gtacttagaa ttcctgcatg aacaggacta aatgatggat
    39721 catcaatcac gcctgtttgg atatagtggg ttgccaaatt ccagccgata ttgtaacaca
    39781 cgataatagc taaaatactc atcgtaccat tattcacgat tgaagcaagt tcagccaatg
    39841 gacttagatc aagtattctt tggactgttg catttgaaaa aatcagagca ttcagcaaaa
    39901 ccatgacaga agcagccatt actaacggca tagtaacgat aaaactgtct cgaatggcca
    39961 gcaagtgtcg ctggctactc atcttagata cgattggtag cattttttca gatagtctgt
    40021 ctacgacttt cattatttca tctcctagat tctaaaattg aaccggttca tttttaatat
    40081 agcacttttt ttaaagattg caacacattt ttttaaaacg ctttctaaag gtggtggatt
    40141 ttttatgact tttgaaaaat tattctattt aatggtattt tgtgaatttt ttctcttcca
    40201 tccgtttgaa gttttacttt ccaatggtat aatttcttta tcctatcaaa tgggggaagt
    40261 attttgaaga aattaatcgt actatttatt ttgtctgcgg gacttttgac attgactggc
    40321 tgtgatttgt ttggcggctc tgaccaatcc tcggacgaaa caaccgattc ttctgtctta
    40381 aaagcggaaa aagcagatat caccaacagt gtatatacgc taaaagtacg taaagatatg
    40441 gatttatccg cgggacagtt gacatttaca gataaaaaaa tggaatggac acgcacgtat
    40501 cagacacaag gagctgattc ttctgaatca tctgaagaaa aaacagtgct gacagatatc
    40561 aagattacta ccaaagatga cgaatatatt atttcaggaa aagaaaataa aaaagaagca
    40621 acagtcacat ttaccaaaat cggtaattac cgtttgaagg ataaggatgg caacatctac
    40681 agtctatagt gccatctgtt ggatcttctt ttgtttctcc ccttcttttc tgctatagta
    40741 aagaagcaag atgactgtaa aggagacgaa atggatgatc gatagtcctg attattgtac
    40801 atgtaataac aacaaaaata tccatacaga atttaatgat caaggaagtt tgagtatatg
    40861 cgacaattgc cacaaactag tcaaagattc ttttaaatcg tttgaatctt atcctgtcaa
    40921 aaattatgta aaaagcaaaa gaagataagc aaaatcaagc aaaactcact atggctgatt
    40981 tttatgaact tcaaactcag cttcttcctt tgatcaagct tcaaaaactg gaatcagtaa
    41041 aacaaacagt ggatcatttc agttcacaaa tcaaagagta acgttttatt tatttcacat
    41101 ctttgaatat aaaataacca aacgatagaa agacttattc tacatcacgt agtaattttt
    41161 ctttcccgtc gtttggtttt ttacttttag cctaattctt gacctttcgc atgctgatta
    41221 tctgtaaaat attttccagc aggctttcct tccaattcag ctaatttttc ttggatctga
    41281 cgaatcacct tttttgcatc ttctggattt tctacaaaat cgaatgtatc cccatccact
    41341 tgtattttag gacatacatc aaaatcctca taccattgat tatagcgggt gttcagtgtt
    41401 tcgtaatagg tgtataacgt ttcatcgctt tctagctgtt catagtcacg cccgcgtttt
    41461 ttgatacgtt ctagcatcgt gtccaaagaa actttgatat ggaccatcaa atcaggacgt
    41521 ttttttggag caacatcatc taactcgttt agcatcgtat ctaataagct atcgtactgc
    41581 tgtacttcga tatccgttac ccgacctaaa tcagcattta gatggaacaa cagactatct
    41641 tcgtagatgg agcggtctag cacattgtca tcgttcacta aagcattttt cattgctaaa
    41701 aatcgcttat tcaaaaagaa gatttgcaat aaaaatgcgt actgctcagg atttgaataa
    41761 aaaagcggca atacttcgtt atcttcgaca ttttcataaa atggtttaga atcaatctct
    41821 tttgcaagca tatccgtcaa actgctctta cctgcaccaa tcgttcctga aatcaaaatc
    41881 actgacatac aacccttcct cttctataag tactcgccta cgtaagtttc aacaggcaat
    41941 ttttcaaaat catattcacg tacagcttgg tctgatttta aatacgtgtg aagactttgt
    42001 gtaatcatca agttcgtagg taccgtctct tcttgtacga tgatcatcgg ttttttcaac
    42061 atagttgcca cacctaattc gtaagccgtt cctggatcga ttgtttgatg ctcaaagtca
    42121 aggatagcca aaacaaattc tgcatttgtc aactcttcca tatctttttc gtatacttcc
    42181 tgcgcccatc cagcgctgaa tagttcataa ttgctttctt ggtgatgacg tggagagtag
    42241 aaacttgtga ttgttttgtt ttcctccaac gctttttcga tacgacttac tcgatcgatt
    42301 tgctcctctg aaaaaaatgg tcctgctaaa tatatttgac tcattatttt gcctccttca
    42361 ataataactg atttttaata gatactgctc gtaattcatt gaccagttcc aatggattgt
    42421 tggaaaagat cacttgatcc aaccattttt tatccataaa atcctctttt gcaaaagttt
    42481 ctaataattg ttgcaagccg ctgtaataac cgccaatatt caatattccg attggcttat
    42541 caggtgtcag gccgatcgct atccaagaaa gaagttgcga aaattcctcc aatgtaccta
    42601 gacctccagg gaaaacgagg acagcatctc ctttttcaaa taataatacc tttctttcat
    42661 caattgtctc tgtaggaata aatgtagtaa catctttccc aggcagctca gtttcaaaaa
    42721 ggccatttgg atagatacct gtgacttttc cgttattttc cagtactgtt ttactaatca
    42781 ctcccataat cccggagtat gaaccaccgt aaaccagttc gatttcttct tgtgccatat
    42841 attttcctaa cgtttgcgca ataaatttgt aactttcttt attgccaaaa cgggaaccgc
    42901 aaaaaacagt aagtttcata aaaaaacact ccctaactca aaagtctatc ttcattctac
    42961 acgtgttttt ttaaaataaa agactagatt ttgtagtttt ttagtggtat atagatcaaa
    43021 caccactata tatagtattt gcaaaaaaat aatttttcag ctattttaca gtaaccaaaa
    43081 aaataaataa ctagtaaata taaaattgaa acgacttact tatagaagaa attgggattt
    43141 cgtcctgcat gtcaataaca cgcattattt aaatagtcac acacttgttt ctcctattaa
    43201 tgaaaaatcc attttattct cgtgttatgt ttcaccaacg cctgatgttt ctctaaataa
    43261 aaaagccgac gaaccaaata atcgaaattt gatcattttg gttcatcagc ttgttcccaa
    43321 acctttgtat taacgataaa tagctggatt cgtaaaacta tcacttggat catcactgct
    43381 ggaagaaaat tcggtgataa ccgcgcctgt tgcacctgcc tggaaccagt gtttaatatt
    43441 ttgttcgatc gtgtactgat ctccagcgtg aagtatgatt tctttttttg ccgtatagta
    43501 acgttcttgc ccctttggaa taatacagga aagttttttt gtttcttttc cttcgacata
    43561 aagatatact aagccttttc gacaacgaaa ggtttccttt tttccttcat tctcatctcg
    43621 aggtgggtgc cgatgctctg gacaagtttg ataaggcaat aataccagtt ctttcccaca
    43681 gtatcgctca ttattttcat aaacaaataa attcaatcct tcagtctcga ttgctcctaa
    43741 tccaaaatct gtatattcga ttctttctaa ttctgtttct ttcaatacta ctcctgaatc
    43801 taggaacatt tgttgtactc ttttcttgta atccattctg ttcctcctaa ctgattgtca
    43861 agtcatccca agatatctct tcttcctctt tttcttcttg gatgacttct gcatttgggt
    43921 caactggttt tttcaataat aaaatagtta ttcccgtagc aaaagaacca gctaacaatg
    43981 caccaatgaa tgcccatggt ctggtcatcg tagcgactgc aaacattccc gatgccggaa
    44041 tacggctatc tgcgccccaa aacatgctca atccaccgcc gactgcccca ccgactcccg
    44101 ttgctatgac attgcgtgcc aagtcgttca atacgatagg gatacatccc tctgtgatct
    44161 gacagatccc cataggaaaa gcggtcttca atgtatcgat ttcttgacga ttgaatatat
    44221 ttttacgaat gattttccct aagaaataag cgatcgtcaa gccgaatggg gttaccatcg
    44281 aagctaaaat caatacagtg attggttcat agattccttc tgaaaatcgt gccacacccc
    44341 cttatccaga ataaatggta atccagaaac agccgggtat ctgcggtaat aagcctaacc
    44401 aaacgaaaaa tatgagaagc taatatgaga agctattttt gtttggctgt tcttattact
    44461 tgatgcaggg cagctttttc acaacctctt tttttattta tgtttcataa tcaatatggt
    44521 aaggatgcag ctccgtatgg tagaccgcct ccgaatattc aatcatttcc ccaatttgcg
    44581 tagacgattt ccgtatccgt ttgatgccga tctgtgcgtc tgttttcaga atttcttgtt
    44641 cttcttctgt taaaaatact gccgagaaat catcagaaaa tgagtcaata gtatatcctt
    44701 ttttcgtcag caaacgatac aaagattctt tgccaaattc ttctctgctg aattgcatca
    44761 tttcttgtgg aagataatga acaaaataaa tataaggctg attgttcaaa taatataagc
    44821 gcttaaaaca aacagcttgc tcaccgaaaa aggaaaacaa aggatcttcc ttctttatat
    44881 ggaccaattg caaatccagc gtttctttcc ggacttcata tcgggagttc tccaagattt
    44941 gcgtaaagct tgccgctttg gataatcgat tgtaaggacg attgcttaga accgtcgtcc
    45001 cacgaccact tttctttgcg acatattcat ctgatgccag cagttcgatt gcctttcgga
    45061 cagtgatttt gctgacttga aacagttctt ctaattcact ttctgtcggt aacatcgaac
    45121 cgactggata ttttcctgag aaaatatctt ctttaatttt atctgcgact tccaagtata
    45181 gtacacgtgt ccttttcata acgtcaccct ttgctttact attactaaaa gaatagcaca
    45241 taaaaaataa aaaaagaaca caaatcacat gtgagaagtg tgttcttttt cattcagctg
    45301 ctttaattac aagtttacgc catttgaggc aattacttct ttataccaat caaaagattt
    45361 tttctttgat cgattcagtg tcccatttcc ttcatcgtct agatccacat agataaagcc
    45421 gtaacgttta ctcatttctc ctgttgatgc actgacaaga tccatacatc cccaaggcgt
    45481 atagcccatc aaatctacac cgtctgctac tgcattagcc atcgcttcga tatgacgacg
    45541 taaataatcg atccgataat catcaataat cgtgccatcc tcttccatat gatcaatggc
    45601 tccaagaccg ttttcaacaa tgaataatgg tttctggtaa cgattgtata attcattcaa
    45661 tgcgattctc agcccttctg gatcgatctg ccagccccat tcacttgcct caagaaacgg
    45721 attttttact cctcccagca agttcccttc tgaagttgct gcttgtggat cagtctcatt
    45781 gattgccgta gacatataat aactaaatcc gatgtagtcc actggatatt cttttagcaa
    45841 ctggaagtct ttttccgttt gctctaattt ttccggatcg actccatatt ttttatgggt
    45901 acgcgctgtg tatgcagggt attctccacg tacttggaca tccgtgcaga aaaaattgaa
    45961 ttcttggttt tggatcattg ttgctgcctg attgactgga ttggcatcga tgctgtaagt
    46021 tgttgcatag ataatcatgc agccgatttg gatatccgaa cgcaattcgt ggccgatttt
    46081 tacggctttg ctacttgcaa cgaattgatt atgccatgct tggaaaatat tttggtactc
    46141 tcctgctcca ttgcttttta ctaatccttg acttaaagct ggaaagtgaa aggccgaatt
    46201 gatttcattg aaagtcatcc aatatttgac tttactgctg tatctctcta atactgtctg
    46261 agcaaatcgt tcatagaagt cgatcagttt tcgatttttc cagccaccgt attcttttgc
    46321 caaatgaaga ggcatttcgt aatgagaaat cgtgatcaca ggttcaatat catattttaa
    46381 gcactcatca atcagctgat cataaaattc taatcccgct tcgttaggag taccttcgtc
    46441 tccatttgga aaaattcgtg tccaggcaat cgaaaaacga taacatttga atcccatttc
    46501 cgcaaacaag gcaagatctt ctttgaaacg atcgtaatga tcaattcctc gatgattcgg
    46561 gtaaatatat ttttcttgat caatcgtcca gtcaaattct tcactgccga taatcgcaaa
    46621 gcgctgctta cctccaggca tagcatctgc aacggatagc ccttttccgt ctcgatcata
    46681 agccccttct aattgattgg cagcagttgc tccaccccat aaaaattttt ctgaaaaacg
    46741 tgattgcatc ctgatttcct ccttatttta caattgccag aacatcttcg tttgcaataa
    46801 tctctttttg gtctaattca agtatatcgg caaatgcatc agtattcgta atgacaactg
    46861 gagtgattac gctatagcca gctgctctaa ttgcttcgat gtcaaaacga atcaataaat
    46921 ctcctgcttt gacgagatcc ccttgtttga ctaagatctc aaagccattc ccatttaatt
    46981 ctactgtatc catcccgata tgaatcaaaa tctccgctcc gtcagtcgtt gtgatcccta
    47041 cagcatgtcc agttggaaaa atagtcgtga tctctccgtc tgcaggtgca actagttctc
    47101 ctacttctgg atcaatagcg ataccttttc ccatcacgcc agaagaaaaa acttggtctg
    47161 gtacttcact caatggcagt actttacccg ttaaaggact ttttaaggta tgccgtgctg
    47221 aagtaatcgg ttctcctgca ttcgcatgtt tgttttctaa ttgattgtct tgtgtttgtt
    47281 cgtcaaatcc taaaatcaat gtacctacga atgcaagaac aaaagcaatt cctgtagcta
    47341 ttactgctac tgtcacattt gactctacac catccaccgt attaatgaaa gtcggaatcg
    47401 tcaataagct gaccaaactg ctggagaaag cttttactcc ggaaaatccg ataatagctc
    47461 caccaatacc tccagaaata caagcggcaa tgaatggttt cttcaaaggt aatgtcacac
    47521 catacacagt tggttcagtg attccaaata acgaagtcac tgtagaagaa atccctaatg
    47581 ctcgaagttt cgtatctttt gttcgcaatg ctacagctaa agcagcccca ccttgtgcca
    47641 agatggctgg aagaagcatt ggcatcaata catcaaaccc atattgttcg atattcaaaa
    47701 acatgatcgg tacgaatccc caatgcatgc cgaacatcac gaatacttgc cataaacttc
    47761 ccatgattag acctgctacg attggactaa atccataaat cgagttgaag agaccgccta
    47821 aagcattacc ggcaaccgtc ccgattggtc ctaaagcaag aaatgttaat ggaaccatca
    47881 ctactaaaac gacaagcgga actaaaatca tttgcagaaa ttgcggaatc acttttttta
    47941 cgaatggctc taatttactt tgaacccata cggctagaat gattggtatg acagaagaag
    48001 tataaccaga tggtgaaaga atcactggga tcccaaagaa actgatagct tctcccgcac
    48061 cagcaactgc tgtgatagct ggatacacaa gtgccatagc aattgctact gctaaaaact
    48121 ggttggcttt gaacttttta gctgctgtaa aagcaagcat gacaggtaag aaggtaaaga
    48181 caccatctgc tgcagcaaac aatatttgat aagtaccgct atcagctgtc agccagccca
    48241 tcaccgttgc caatgataag aatcctttca ataccccggc ggctgccatc gcccctagaa
    48301 aaggtgtaaa tatactagag ataatatcga tcagacgatt caaaatattt ttggactctt
    48361 cttttccatc ctccgaaact gcaagacctt cctcatccac gatcgattga taaacatctg
    48421 ctacattact gccaatcact acttgatact gaccgccact ttgtacgact tggatcacgt
    48481 ccgggtcttc ttttaacttt tgcgtatctg ctaaagattc atcttttaat ctgaatcgca
    48541 accgcgtagc acaatgtact aaacttttga cattcttttg tcctcctacc gcttcccata
    48601 cacgacgacc aactgcttga ttatccattg ttttttcctt ctttcttttt tatttaaaaa
    48661 ataaaaaccc agattgaaaa aaacagtcac aaacattcgt gaacctattt tttcaatcca
    48721 ggttttgcct gccatacagt aacaatcctt atgattggat gctttttttc tcacttacta
    48781 ctctttgaat atgaatcgtt aaataaatct gctctgcgat ccccatttcc tgtttttgcg
    48841 tttgtcgaag atactcattg atccgttcga cacaatgaaa cgcttttgga tactttcctt
    48901 gaactaatgc gtagagaaat tcatcttgct cgtcagatcc ttcccctttg agataacgct
    48961 gggcaaaaaa ctgcagatga gtaatgatac gctgataatt caaagattct tcatcgaaca
    49021 cgatgccgaa gaatttactg atgatactca gtatatctct taccattgtg gtagcggtca
    49081 tcgtcacttg catgtcttcg tttccttgtt ggctattcac aaaatgaaag gcaataaaac
    49141 cggcttcatc ctcagaaagt tccacacccg tcttttgttt cacgaacaaa agagcctctt
    49201 tcgctgcttg atattctttc ggataaaatt tttttgtttc aaataataat ggattaggca
    49261 gttggatatt ttcttttgct cgtgtgatcg cataatgaag atgatcggtc aatgtcagat
    49321 aaatattagg cgaaaattca acttttagtt tatcttcagc cagttcgact acttgtttga
    49381 cccattcgat ctcctctgcc ggaatatcag ccatcagctg ctggaattgg tttttagcaa
    49441 aagaatctct taaaacaaat tctttttgga ccgcttctgg cgaaatctcg tccccaatct
    49501 ttttttgaaa agctagtcct tttcccatac aaacgacttc ttccccgaat ccatttttcg
    49561 aaatgacgac attgttattc aaaattttat caattttcat tttgcttcgc cctctcaaga
    49621 aaaataaaaa aacctagaat tcgcctactg tttcttagct gaattctggt tttgcctgcc
    49681 gtacagtaac aatccatttc ttattcaaaa tcatcatacc atcgaatttt tatttatgca
    49741 agccttttca tataaaaaat aaaaaaactg gaacacaagt ctatctagaa aacaaccaaa
    49801 tcagagatgc acctctttgc gtttctcttt ttttcagcaa attggttttt ccttatcgat
    49861 tcatatcctt tggctgtttt taatctgtaa cgacttatgt cccagtatcc taaaatgttt
    49921 ttgtctctag attgaaagca gatctcatat catctgtcgc atcttttatc gatgtaacac
    49981 tttccagttc tccttgtaca actagtcgcg tgacggcgtc catgtcgcca caagtaatta
    50041 gtgtgacaat ttcttttccc tcgacttcat tcagtaattg ggtatctgta ggagcaactt
    50101 tttctttaaa aaatgttttg tatgtataca cattttctaa atctgtcaaa tagatcttgg
    50161 cacccatctc taaattttct agcggtgtaa acagcaattc agggttcgtt gcacgatggc
    50221 ttgctaaggc ataatttcct tttcccattt cttgatctgg agataatgtt cctgctccgt
    50281 aaagtagtga cgtattatcc aagcctttga aaaccggtaa attgattgaa actgaaggga
    50341 ctgcgatact agcaatcact ggtagatctg ttcctctcat ttgcgacctc atgacccctt
    50401 ctgtcgtcat tggttctacc gcatcaaagt caaaactgac atctttatcg ttattctttt
    50461 tcagatcttc ttttgttaca ttcgctatag catatttatc tcctgtctct ctgacaaaat
    50521 aatccttgat ctgttcattg aaaatcaaag ctagtcctac taataacaag aggaacaaga
    50581 ggatattgac tagccatctt tttgcttttc cttttttttt cattttctct ccctccagac
    50641 tcattttact gtttttcttt tgctctttcc aacagccgaa tgacacattt ctacaactct
    50701 gtgtattttt ttcgtacacc tgcgcttttt tctacaggat atttcaaatt atttttgatc
    50761 aatttttctt caatgatctc atctagatcc aggtgaaggt tgtctgccat catgtatgaa
    50821 tagatcaata catctgccag ttcttctttg attcgttcta attgtgtttc ttttacttct
    50881 tccggcgttt tccattgaaa aagttctaac aattcagatg cttccaaaga aatagagata
    50941 gccaaatctt tttcgttatg aaattgtcgc caattacgtt catctctaaa ctggttgact
    51001 ttttccatac tattcatttg cttgccttct tccaatcact tcttacttga ttaatcatac
    51061 cattttttta cataaaaaat agatgatgct tttaaaaaaa ctcctgtttt cttttattta
    51121 aaataaatgt catagaaaat caataattta ggtagccacc atgtgtatgc agtagcgtgt
    51181 cttcttccct tgaggctttt tgtttttcga tacgttcttc ttcccaaaca gacaacgcta
    51241 ctagaatctc tcttaatgat tttccttctt cggtgagcga gtactctact ttaggcggga
    51301 cttggttata ggcttttcga tggatacttt catcttgttc taactctctt aactgctggg
    51361 tcagcatttt tttgtgatcg tagggatttg cttgcttctc cacgcctaaa ataagcgata
    51421 ttgctcttcc ctttatctgg tgtaagcaaa gcagttagca ttttgacgat cgtgggttcc
    51481 gcttttcact tcttatctac ttagtatcat gaagtatact ttcgtactaa tactaagtag
    51541 cggaagtgtc aactactttg tttaactaca tagcaaaaga aacagaaggg tgacaacggt
    51601 catcacgctt ctgtttcttt tgaatatgct gtgttgttgt ctctatctcc gtgtagactt
    51661 tcctcaaact tatcctttga cagtaagtca gtaggctgat ttgctctttc tagatgccag
    51721 atacatttct acaaaaaaat agtgattctg ttttgaaaat tcgattttta gttctgcatt
    51781 ttcgtaaaca tccaacattt ttttcgcact gtatagacct aacccgccat tttttttaga
    51841 ggtttttcct cttttgacca tctccgatag cgtcaaattc gttggatttg ccttgttcgc
    51901 tatactaaaa cgtgatccct ctttttcttc cagcagattg atcgtaatcg gactctgatc
    51961 ggctacacga tgatcgaatg cgttagaaat aaaaatagat aaacagcgaa taaaaacaac
    52021 cagatcaatc gggattgttt tcacttcgac aggaataact acttggatcg ggattttctc
    52081 cgattctgct ttttctagaa attttaaaaa gacacccctg acagctgggg atacaatatt
    52141 tgttagctgt tgtatttgcg gtttttcaaa aatagctgca gaatgatctt ctaatatttc
    52201 taaaaattcc aacgtttctt ttttattttc ctgaagagta atttttaatg aaaacagtag
    52261 acttttataa tcatgtttga attcctctag atgatggtaa tattcttcat ttatttgata
    52321 cagttgaagc gattcctgat gttgtctttc ttttacttgg aaactgatca acataatgga
    52381 aaaaaacaaa gaaaaaaaac tgatgactat cgacaaaaga gatagaagca gataattaga
    52441 tgagcggaag tgaacacttc ccaataccaa gtaaatcacg ccccagctta ataaaataca
    52501 aatacctcca aaatagtgtt cagttggata agtaaaaagc aagttgagaa atctcgtctg
    52561 tttgtctata ctttttacta atagatagaa aataaatgcg ataagtagtg caaaaaaact
    52621 tgctgttcca agatgtctct ttcctaaata atccatcaga tcaaaaacca gaaaaaagct
    52681 gagtccagtg atagcaaaaa aaagattact gatcaaacag ctgataaaca ggtttttcgt
    52741 agtaaaataa aaaagaaaca tcaataataa gccaattagg taaggaaagc tcactttagg
    52801 gataggaagt aaaagtgtca ccaccatcaa agctatacaa ccaaagctgt tttttactag
    52861 cagttgtttc gaaaagaata aatagatacc taaatctaaa atcaaagtaa aggtcaaata
    52921 aagtgtcact gttgcgacca tcttattcct ccattgtttt gtttcaaaat gtacacatat
    52981 ttttttaata ggagcctgca tatgaaaaag tattttatca tagaagataa ccagtttcac
    53041 agcgaatgga tcatcagaga attggtcaat catcatatcg agaatatcgt gcagctcact
    53101 tctatctctc aaatcaatca gttgatccag ttgggcacta ttgataatca cgatgttttc
    53161 tttatcgata tcgacttgaa gcaatcactc acaggtattg atgtcgccga acaaatacgt
    53221 aaaaaaaatg cacatagtaa tattgttttt ttcaccgctt atacgaattt tgcagtagag
    53281 atcatcaatc ggaaaatcct tccgattgga tatatcagaa aagacaggaa tttatctgaa
    53341 caaattcgtc aagtgctaga acagatcaat tggaaagaac agcagatcgt aaatgtcgat
    53401 aaaatcttta ttgatcaaaa aggtggaagt ctcgtcctgt atcctcggga aattttatat
    53461 attgaaagta tcaaaggttt gaaaaaccaa gttatggtca aaacaactac agaagataaa
    53521 ttagtgaata cttctttaaa acaattgaag acagagttag aaaagtatgc ttacttttta
    53581 cttttaaaat cttactgtat caacttatcg caattgcaga aaattgattc tgctcattat
    53641 gtgttagaat ttttcaacgg tgactcctta ctcgttggtc gaaagatctt tgaaaagaca
    53701 aaggaacgtt tccatgactt ccaaaaaact gcttcgagtt gatgaataag agctgagacg
    53761 taagcatagc agatttcagg gagtgggcct tgaatcggct ccactccctg aagtcgttga
    53821 tatgatttta cgcttcctct tctgtaggag catctgttaa cgaccatgtg aatgttgaaa
    53881 cgtagttgtc tacagcataa ttggatacag gaacaaataa ttccactgat tgtctccctt
    53941 gttccacatt ctctcctaat gaataaatcc atgtgccaat cccttcattt gctcctgcag
    54001 ttactagatc tactgcctga tctgctggga tttcgattcc ttcagcgcca gttccagaaa
    54061 cggtagatgg cattccactg ctattagttg cgctatttcg aatcacggcg tttttgaatt
    54121 tcacctttga accagttagt ttattcgtat cagatgttgt accaataaat tctgttcgtt
    54181 tgactgataa tttccaaccg gcataatttc cgcttttatc cgttacttgt acgaaattct
    54241 gtacttgtgt ttgtgctcca gtactgtccg ttccgctttg taatgcagca tatactgttt
    54301 ttgctgtacc taacttgatt gttccaaaat caagatctgg agcttggtca atcgtcagta
    54361 atccggatgt tccctgattt ggttcgtcag gtgtaaaagg agtatcagga tcatttggat
    54421 caacaggagg aactggaaca acgtcttcag gatttgcagc atctggtttg gtcacagaaa
    54481 tcgttccttc tgtagtggct gagctcttat tctcagcaaa aacactttgt gtacataata
    54541 atagacttcc agctagaacg gtacttaatg caattgtttt tttcatttct ttttccttct
    54601 ttcttttaaa ataaataatc cagcaaataa gccaataaga atgactccaa caagtatcag
    54661 ccatcgccag tcaaatgttt ctggctgatt ttttaaatca atatcctttt cattcaattg
    54721 gtccgctgca tcatcagtta tttcaaaatc ggtatggaga ttccagcgtt tttctttcca
    54781 agcacccttc acaaccgctt gataatgtcc cggagctaat ttttttcctt ctaatgggac
    54841 aagtaaatca aaatttgtat tgggtgctac atctattttt tcttttttta ttgaaaggta
    54901 aggctctttt gtttcttttt tgtagatttc aaccgatgta gtcaagccga acatataaga
    54961 agcatttgga ttttgaagat tcgccttaat cacatttctt ccatcgcttt gatccgcata
    55021 agcttttttt aatttcaaat ctggggaaga ctctttttcg ctatttcaaa tcaagattgc
    55081 tttcacttga gaaaactgat tgacgacacc aactgtactt ttgttagaaa cgttctcttt
    55141 tttttcttga aacgtgatcc ctcctgcgac cacaccagcc agctcttttt ctggcgcttt
    55201 cagtgaaagt gtctcagtga ccgcactttg aggaggaaca gttatttctc ccactgtttt
    55261 tgctaagttt ttcatatcat aacttaatgt ttcatcaaat ttactttttg attctccata
    55321 ttcgatcaca ccgtttgaat tcgtcgtagc ggagtggatc aatgtctcaa ctgttactga
    55381 tttatctgtt cggttaaaga ccgtgtaggg gatttcctgt gttttcccag gctccatcct
    55441 tacatttata tatgttttgt tcttatcaat ctgattagcc ggtaattcaa aatctaccgc
    55501 aaaattgaat tcggaggcat ggacaggtgt ctgccaaagg atgaatccaa ggagaaggat
    55561 cataagtaaa gagcagttgt ttcgatgact tttattccat attcgattca tcaatcatcc
    55621 tccttgatct ttattctgtc ggcgcatctg ctaatgtcta tgttatctct gctgagtaat
    55681 taccagctat ctgattttct ttggttaaga caagtttaaa cgcccgaccg ttatttaaat
    55741 attctgaata atctgcttgc acattcgttg aattttgtcc tctgacaatt tccagttctt
    55801 cttcattcac taattgatac tcctctcctt tcttgaaata taggacatcc tgcatagaaa
    55861 atcctgcatc atttgtgaaa ggctgagata attttatcgt taagatccac ggatttttcg
    55921 tttctcgctg atcataaatc cataattttt cctgtatatc catttggcca aatgcttctt
    55981 gtgtcaaacc agttgctttg atctgaccaa aatcgagttt ggaaggggca agaaatgaga
    56041 gaccaccttc tactacgatc ttcgtagcag gtgcaaaagg cggggcatca tgataaacaa
    56101 ctggcgtatc acttggattg tgattcccaa tttcatcgtt ggtacttggc ttgttctgca
    56161 cacccttagc agtagcttct cctgcttggt cgtttaatac aatttctaat acatctcctg
    56221 caaaaagttg attttctggg atagaaagaa caaattcccc tgtatcatca atagttgttt
    56281 ctgacaaaac agcttgattt tttttcaatg aaaccaaggc tcctgcttca cctgtacctg
    56341 tgatcgtgcg agaagctgca ttgatcaatg tggtacttaa attagcaggt tcaggaggaa
    56401 tcacatcgac tgtcgttttg gcagagtcca gttctgtcac ttcttggcca cctcttactt
    56461 ttttagcagc aacaacttgc acttgatcct ccgtttttaa tctctcatca ttgggtactt
    56521 gaacttcgaa caagccgcct tgttcgcctt ctccccatgg gttatatcct gccccttcat
    56581 tttcatctgt ttgtccttgc gtgagagcag taatttctac agatgatttc gtcgtatctg
    56641 cgtaagtaat acgtaaagtc acttctactt cgcctgtcca agcatctctt ggctttgcct
    56701 cgacaccttc gggaacaacg acatgcccat agattttttt atctgcattc gttggcactc
    56761 ttaaggattc aatcatcggg agctgattat tggcactgat tcgattataa tttttcattc
    56821 cggcacctgt ctgagcaaat tcatttgtta aacctgcact agggcttgat acgaaattgc
    56881 ctaagtcggt cccattgatc gataagtctg tcatatcaga aaaataatcc gggtcttttt
    56941 caaagttatc gaatgttgcg cctgtttttc gccataaagc gatttctgaa tgggcgatct
    57001 ttaaagaaga atctccattt ccttggaata cccagccgcc tatgccattg gtctcgcttc
    57061 caatccgttt gttttggaaa tcaaaatagg taggagattc taagtccaca ttcaacacat
    57121 ccccactgat gatgccggtc ttatcttccc ctgttttccc gaccatgact aaataagttt
    57181 tttcacctgc cgtaaaattt aaatcaatat ctccagttgt atctacagtt gctccggaat
    57241 ctgcgcgtat atcgatattt gatttttctc cagtgactgt aaaatcagct gaacctggac
    57301 tttctagaaa accagaagcg tcataaaatt ggatcgcttg atttccttga ttgagtctgt
    57361 tcacgtaaga tttgccatca cctgtatttc ttaccctcaa tgagcctcca ttggttactg
    57421 tcagcttttg cgtaccattc tcaaaacgaa ctgcgctgga acgcccactc tgcttatcga
    57481 tgtcgataac tgatccggct ccttctacga tcaaagaaag gccttttcct acaaaacgca
    57541 tcccattgtt cccgggttga tcgtcatctc cagccatttt gaccgagatt tgtccaccac
    57601 ttgatacatg gatttgacta gaatctcctt gcaagcgaaa tccgcccgta gttgctgtat
    57661 tgatggtgat tttcccacca ttcgatacag tgactttcga tcctgaacct ctcatttgga
    57721 tgattccgcg attgttgtca ttggtaatat tgatctttgt atttaccgaa aattcgcttc
    57781 ccgaaccact gacttcaaac atgacattac caggtagcgt atcgactgcc tctccttcat
    57841 cgctaacgcc atctgcccga tcataatcaa gagagacctt tgaaccgctt aagatatgga
    57901 ttcctggcgc tgtttttcca ctaccagtct catctcctga atctagaaaa ccgtagaatt
    57961 tccctagata cggtccataa agttgactac cattgatcac attcaattga tagcttctac
    58021 aaaaataggc tttacccgga gcggcattca gtccttcctt cactggttgg acatccaaca
    58081 caccggaaac accatctaat gtgacagttg cacgaggagc gtaaatcagt cctaatttgg
    58141 acccatcata caaatcaagt gtcccggtaa attttatttc tcccgtaaaa tgatatctgc
    58201 ttccccaaaa atcggtcata taagcaatga taatggcatt tccatcgata tttggtgtca
    58261 atccacctgg atgtgcagct gttcctttaa aattctggaa ctcgatcgac tgaggtatct
    58321 cattgatata aatatggtgt gtgccgaaat tgtgagcgaa tccaccaaaa tcaataacga
    58381 tatctcgttt gatctctaat tccctgtcta aagtcaaagg ctgaatcagt cggatcttag
    58441 ttactgattc gtctgcgaga agctgcgcca gttcttctgt tgtggcagct tcgctgtacc
    58501 catccgctga aacagctctt gccagtgccg cttcactgct atcagacgtt tgttcagcta
    58561 cttctgaggt ctcacttgtc ttattttctt cttgcctttt attagtagaa gcctcacttt
    58621 gagattctgt agtttcagaa gtaatcgatt cttctgaaaa agtagtcttg tcaaaacttt
    58681 gattctctga ttgcgttgat tcgtttgttt tctctacatc ggcaaaaacg gatgtcacca
    58741 tcaagagagg gaagacaaac aaaatcgacg caatcagctt ttttacacat tgattcattc
    58801 tccaccatcc tctctgacgt tttgacaatt ttattttatc gttttaatct atttccaatt
    58861 gtactttcat atttaaaata tgtattttta tggaaaaatt tttctattag gtgtaaattt
    58921 cttttttgta caaaaaaaag gctgtgacaa aagtcttgtc acagtccctt atcccgaaca
    58981 agcggtgttc tcccgccaac tcctcggtca caacctccat tttaacaata aacctgtaaa
    59041 tgtgagggca atactttgat tgtcacaggt aatttatccc cttcatctcc atcaacattc
    59101 gtcgtcaatt cttcctcatc agctaatgac acaactaatt gctcacaagt aaggtaggct
    59161 agatttttcg tagattcatc tactcctttt aataggtctg gcaatgtttt aagcgtatcg
    59221 atcaaagaag aatcttttaa ataaaccaaa tggagcttac catcatccac ttttgcattt
    59281 ggaagtaatg tttcaaaacc gccgactgag ttagttagtc ctatcagcaa ggtactgctc
    59341 tcaatttctt ccttctttcc atcaacttct acgtgaaacg agtaggattg tgtggaagct
    59401 aactgtttga ttcctgacat gaaataagct agttttccaa atttcgtctt cttctccgca
    59461 tctacatcat tgattgattc agggatagag ccaattgcga cgacattcat aaaatagtcg
    59521 tcatttattt ttccaatatc caatggcttg actgattcaa tcgaaaaatg attgatcgct
    59581 tcctcaggtt ctagcggtat ccctaatgct cgagccaagt cattcaccgt acccaatggg
    59641 aaaaatccga aatttggccg atgttcttgt tcagcgattc cactgattcc ttcgttgact
    59701 gttccatctc cgcccatgac aaacacacta tgataccctt ccgtagccgc ttcgcgagta
    59761 aagttttttg catcccctgc tttttttgta tgcaagacga ccacttcatc aaacaccgat
    59821 tccaattttg ctatcgctaa ttgctcaaat tcctttgctt gttcaccgcc tgaactgggg
    59881 tttaccacta ataaaacttt tttcatcact ttctttccct tctgattgat ttctttaata
    59941 taacgatagc ataaagaaaa aaattaggct gtgatgaagt actgttcatg aaacattcat
    60001 ttattttttg acaagataaa acatccatac tatgaaactg aggaaaatta aaacaatgat
    60061 ataggaaata aaagaaaatg ataggcattg tatacctaat aaagtaaaaa tcttaggtgg
    60121 cagataaaaa cacaaaaata gcaagagaat gtaatagcgt ttcttccact ttttgttatc
    60181 agaccttata gcgatcagag gaataccaaa tgcataactg aaaaactctt gatgtactgt
    60241 taatatgtca cggccttgaa taataaatgc gacaatcccc gctataatta gtgtgcctaa
    60301 caccaaaacg gaaaattgat gaatatattt tatatacaaa tgatccattg tacttcctgc
    60361 ctttttattt gtgagaaaaa caaatccgta tacgtgaata acttctagaa ataaaagaaa
    60421 tttgcaaaac cactccattt atctacttct acgtgcattt tttgatattg ttttctttat
    60481 ttaagattat acagaaaaat atctagggtc gctggattct aagtaaaaaa aatttttaaa
    60541 ataaaaagaa ctaaatcaag tagtaattaa actactgttg atttagttct aataaaaaga
    60601 tt
//
LOCUS       DAZKCY010000004        53799 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000004 DAZKCY010000000
VERSION     DAZKCY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.004"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..1808
                     /locus_tag="ACG8XC_000211"
     CDS             177..1808
                     /locus_tag="ACG8XC_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFP9189942.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1870..2511)
                     /locus_tag="ACG8XC_000212"
     CDS             complement(1870..2511)
                     /locus_tag="ACG8XC_000212"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9189943.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2697..2773
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2793..3101
                     /gene="rplU"
                     /locus_tag="ACG8XC_000213"
     CDS             2793..3101
                     /gene="rplU"
                     /locus_tag="ACG8XC_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP9189944.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            3128..3472
                     /locus_tag="ACG8XC_000214"
     CDS             3128..3472
                     /locus_tag="ACG8XC_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFP9189945.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3485..3778
                     /gene="rpmA"
                     /locus_tag="ACG8XC_000215"
     CDS             3485..3778
                     /gene="rpmA"
                     /locus_tag="ACG8XC_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP9189946.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4212..7019
                     /locus_tag="ACG8XC_000216"
     CDS             4212..7019
                     /locus_tag="ACG8XC_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9189947.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7210..7701
                     /locus_tag="ACG8XC_000217"
     CDS             7210..7701
                     /locus_tag="ACG8XC_000217"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP9189948.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7804..8793
                     /locus_tag="ACG8XC_000218"
     CDS             7804..8793
                     /locus_tag="ACG8XC_000218"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP9189949.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8826..9629
                     /locus_tag="ACG8XC_000219"
     CDS             8826..9629
                     /locus_tag="ACG8XC_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP9189950.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9650..10564
                     /locus_tag="ACG8XC_000220"
     CDS             9650..10564
                     /locus_tag="ACG8XC_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9189951.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10647..11021
                     /locus_tag="ACG8XC_000221"
     CDS             10647..11021
                     /locus_tag="ACG8XC_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFP9189952.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11160..11405
                     /locus_tag="ACG8XC_000222"
     CDS             11160..11405
                     /locus_tag="ACG8XC_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9189953.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11577..12641
                     /locus_tag="ACG8XC_000223"
     CDS             11577..12641
                     /locus_tag="ACG8XC_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFP9189954.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12887..13312
                     /locus_tag="ACG8XC_000224"
     CDS             12887..13312
                     /locus_tag="ACG8XC_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9189955.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13305..13754
                     /gene="nusB"
                     /locus_tag="ACG8XC_000225"
     CDS             13305..13754
                     /gene="nusB"
                     /locus_tag="ACG8XC_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP9189956.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13851..14705
                     /locus_tag="ACG8XC_000226"
     CDS             13851..14705
                     /locus_tag="ACG8XC_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFP9189957.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14706..16043
                     /gene="xseA"
                     /locus_tag="ACG8XC_000227"
     CDS             14706..16043
                     /gene="xseA"
                     /locus_tag="ACG8XC_000227"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP9189958.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            16048..16272
                     /locus_tag="ACG8XC_000228"
     CDS             16048..16272
                     /locus_tag="ACG8XC_000228"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP9189959.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16274..17164
                     /locus_tag="ACG8XC_000229"
     CDS             16274..17164
                     /locus_tag="ACG8XC_000229"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP9189960.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17173..17991
                     /locus_tag="ACG8XC_000230"
     CDS             17173..17991
                     /locus_tag="ACG8XC_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFP9189961.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            18098..18547
                     /locus_tag="ACG8XC_000231"
     CDS             18098..18547
                     /locus_tag="ACG8XC_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9189962.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18565..20247
                     /gene="recN"
                     /locus_tag="ACG8XC_000232"
     CDS             18565..20247
                     /gene="recN"
                     /locus_tag="ACG8XC_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP9189963.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20302..20418)
                     /locus_tag="ACG8XC_000233"
     CDS             complement(20302..20418)
                     /locus_tag="ACG8XC_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFP9189964.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20491..21447)
                     /locus_tag="ACG8XC_000234"
     CDS             complement(20491..21447)
                     /locus_tag="ACG8XC_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9189965.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21618..21986
                     /locus_tag="ACG8XC_000235"
     CDS             21618..21986
                     /locus_tag="ACG8XC_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFP9189966.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22156..22587
                     /gene="mraZ"
                     /locus_tag="ACG8XC_000236"
     CDS             22156..22587
                     /gene="mraZ"
                     /locus_tag="ACG8XC_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP9189967.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22608..23567
                     /gene="rsmH"
                     /locus_tag="ACG8XC_000237"
     CDS             22608..23567
                     /gene="rsmH"
                     /locus_tag="ACG8XC_000237"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP9189968.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23572..23973
                     /gene="ftsL"
                     /locus_tag="ACG8XC_000238"
     CDS             23572..23973
                     /gene="ftsL"
                     /locus_tag="ACG8XC_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP9189969.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23974..26166
                     /locus_tag="ACG8XC_000239"
     CDS             23974..26166
                     /locus_tag="ACG8XC_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP9189970.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGMFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26192..27154
                     /gene="mraY"
                     /locus_tag="ACG8XC_000240"
     CDS             26192..27154
                     /gene="mraY"
                     /locus_tag="ACG8XC_000240"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP9189971.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27168..28529
                     /gene="murD"
                     /locus_tag="ACG8XC_000241"
     CDS             27168..28529
                     /gene="murD"
                     /locus_tag="ACG8XC_000241"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP9189972.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYRHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28555..29643
                     /gene="murG"
                     /locus_tag="ACG8XC_000242"
     CDS             28555..29643
                     /gene="murG"
                     /locus_tag="ACG8XC_000242"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9189973.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29658..30878
                     /locus_tag="ACG8XC_000243"
     CDS             29658..30878
                     /locus_tag="ACG8XC_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFP9189974.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            31034..32359
                     /gene="ftsA"
                     /locus_tag="ACG8XC_000244"
     CDS             31034..32359
                     /gene="ftsA"
                     /locus_tag="ACG8XC_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP9189975.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32382..33623
                     /gene="ftsZ"
                     /locus_tag="ACG8XC_000245"
     CDS             32382..33623
                     /gene="ftsZ"
                     /locus_tag="ACG8XC_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP9189976.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33640..34317
                     /locus_tag="ACG8XC_000246"
     CDS             33640..34317
                     /locus_tag="ACG8XC_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFP9189977.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34331..34930
                     /locus_tag="ACG8XC_000247"
     CDS             34331..34930
                     /locus_tag="ACG8XC_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFP9189978.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            35011..35232
                     /locus_tag="ACG8XC_000248"
     CDS             35011..35232
                     /locus_tag="ACG8XC_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFP9189979.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35281..36063
                     /locus_tag="ACG8XC_000249"
     CDS             35281..36063
                     /locus_tag="ACG8XC_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFP9189980.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            36084..36791
                     /locus_tag="ACG8XC_000250"
     CDS             36084..36791
                     /locus_tag="ACG8XC_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFP9189981.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            37053..39851
                     /gene="ileS"
                     /locus_tag="ACG8XC_000251"
     CDS             37053..39851
                     /gene="ileS"
                     /locus_tag="ACG8XC_000251"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP9189982.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39900..41426)
                     /gene="zwf"
                     /locus_tag="ACG8XC_000252"
     CDS             complement(39900..41426)
                     /gene="zwf"
                     /locus_tag="ACG8XC_000252"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP9189983.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41441..42088)
                     /locus_tag="ACG8XC_000253"
     CDS             complement(41441..42088)
                     /locus_tag="ACG8XC_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFP9189984.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42272..42601)
                     /locus_tag="ACG8XC_000254"
     CDS             complement(42272..42601)
                     /locus_tag="ACG8XC_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFP9189985.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42778..43506
                     /gene="sfsA"
                     /locus_tag="ACG8XC_000255"
     CDS             42778..43506
                     /gene="sfsA"
                     /locus_tag="ACG8XC_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP9189986.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43522..44535
                     /locus_tag="ACG8XC_000256"
     CDS             43522..44535
                     /locus_tag="ACG8XC_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9189987.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44535..45812
                     /locus_tag="ACG8XC_000257"
     CDS             44535..45812
                     /locus_tag="ACG8XC_000257"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9189988.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45875..48577
                     /locus_tag="ACG8XC_000258"
     CDS             45875..48577
                     /locus_tag="ACG8XC_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9189989.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48729..49046
                     /locus_tag="ACG8XC_000259"
     CDS             48729..49046
                     /locus_tag="ACG8XC_000259"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9189990.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            49076..49396
                     /locus_tag="ACG8XC_000260"
     CDS             49076..49396
                     /locus_tag="ACG8XC_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9189991.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49504..50964
                     /locus_tag="ACG8XC_000261"
     CDS             49504..50964
                     /locus_tag="ACG8XC_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9189992.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            51032..51253
                     /locus_tag="ACG8XC_000262"
     CDS             51032..51253
                     /locus_tag="ACG8XC_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189993.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51284..51466
                     /locus_tag="ACG8XC_000263"
     CDS             51284..51466
                     /locus_tag="ACG8XC_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFP9189994.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51466..51879
                     /locus_tag="ACG8XC_000264"
     CDS             51466..51879
                     /locus_tag="ACG8XC_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFP9189995.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            52002..53183
                     /locus_tag="ACG8XC_000265"
     CDS             52002..53183
                     /locus_tag="ACG8XC_000265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9189996.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53254..53418)
                     /locus_tag="ACG8XC_000266"
     CDS             complement(53254..53418)
                     /locus_tag="ACG8XC_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189997.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53572..53645
                     /locus_tag="ACG8XC_000267"
     tRNA            53572..53645
                     /locus_tag="ACG8XC_000267"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53606..53608,aa:Arg,seq:cct)
     gene            complement(53714..>53799)
                     /locus_tag="ACG8XC_000268"
     CDS             complement(53714..>53799)
                     /locus_tag="ACG8XC_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFP9189998.1"
                     /translation="LHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        17764 a         9125 c        11629 g        15281 t
ORIGIN      
        1 ttactcaaac aaggtgggct agctctgttg gcattgcttt ctattttctt ataaatatag
       61 aaaaaataag aatttatcta acaggaaact ttgtgaaaaa tattacttta tctcatgaaa
      121 tatgatattc taatgaatgg tattttagaa aatttcatga aaaaggagtt agataggtgt
      181 tcaaaaatta tgtttcctca ctaaaaaaag aattttcagg ttatgatctt tcaagatttc
      241 agaaagatct tttggctggg atcacagtag ctgctgtagc attgccttta gccttggctt
      301 ttggggtatc aagcggagct gatgcggccg caggtttgat tacggctatc attgccggta
      361 tcgttattgg cggtttatca ggcggttttt atcaaatatc aggtccgaca ggtgcgatgg
      421 cagctatctt gatgtctatt gcggcaacaa agggaatgca aggtgtattg cttgctactt
      481 ttttagcggg tctattttta cttgcagcag gacttttccg attaggaaca ctgacttctt
      541 ttatcccagc acccgtaatt acggggttta cttcaggtat tgctatcatt attgcattag
      601 gtcagatcga taatttattt ggtgttcact cggaaggtac aaacgttatt gaaaaactaa
      661 gtagttatca aacgatagga tttccgataa acacagcctc tttgatcatg ggagggctag
      721 tcattttagg gatgtttatc tatccaaaaa aatgggcgaa acgaatgcct agttcgctgc
      781 tggctatcat tttggcaaca gcagttatgc tattgttcca tttaccagta gcaactgtgg
      841 gtaagattcc acaaacattg atcagtagta atcgtttgaa tatgggagat tttagctttt
      901 ctgccttgca acaagtggca gttccagcta ttagcattgc tttattagga atgattgaaa
      961 gcttgctatg tggtgcttcg gctggccgaa tggcaaataa gcccttagat agcaatcaag
     1021 aattaatggc gcaaggaatt ggaaatctgt tgcttccttt ctttggcggg attcctgcta
     1081 ctgcggccat cgcccgtaca agcgtggcca tcaaatctgg tgcacaaact agattagctg
     1141 gtatgataca tgcagttgtc ttactgctct ccatgcttgt ttttgcgcca gtgatgtcac
     1201 agattccaat gccagcactt gcaggtgttt tgattgtgac ggcttggcgt atgaatgaat
     1261 gggaaacgat caaggagcta tttactaata aatattggtc agcagttcta ttgtttatcc
     1321 ttacgatgat ttgtacagtt atctttgatt taagtattgc tatcgtgatc ggggtgatcg
     1381 gcggctgtgt attttttgtt gtcaaaagcg ctgctatcac gatttcatta gaaacgatcg
     1441 attggcaaag aatggggctg ccagaaacgg aaaagctgaa taactggaca gtagtgtata
     1501 ttagtggtcc actgtttttt atgtcttcag aaaaactcaa aaataaattg gaagaactaa
     1561 atcatcacga tggtgtcatt ttctccatgc gaggcgtacc aagtattgat ttgacggcac
     1621 gtgcagtttt tgatgaattt caagaaaaag cagagcaaag aggacaaacg attatctata
     1681 cttccttgca gccagaagta gaaaaacaac tttctcactt gtgggaacat acattcacgc
     1741 atcaacatca aacagttgct catgcgttga cagctttaca tgcgaaatat actgcaatta
     1801 gccattaaaa caatgaaaaa gaacagacaa gactgtaaac tgaagatttt atggtcttgt
     1861 ctgttctttt taatattgct gtaaaagtga agctagtact tgccagtctt gatggataac
     1921 aggtacaagg gagcgattgt agaaaatagc tgctggatga aaaagtggaa gaattcgata
     1981 gtttttttct gaccattcgt aggcatcctt ttgttctgtt aattgtaaga caggccctgt
     2041 atagagttga ccatgatttt tggtaaccgt atattctttg ccaagtaaac gttgaagacc
     2101 aatgtttcca agtgtcgcaa ttaattttgg ctggaccttc atcagttcgt aatccaatat
     2161 tggtgcatgc gcaaatactt cggaacgagt aggtgtccga tttggataaa cagtttctga
     2221 ctgttgtgtc cgtttattga tccgatgagt cacgcgatac gggcggctcc ttacagcact
     2281 ggtgatatac acctcttctc gtgttaagcc agttgaagaa agtgcctgca tcaattcttt
     2341 tccagcttga ccgctaaaag gaacatgcgt atcaatctct gttttccctg gggcttctcc
     2401 cacgagcatc agtttaggat gaagaggtcc ttgcccagcg ataaatcctt ctacaggacg
     2461 gccgttcatt cttaaagaag cttcttgtat cagttctttt gggtattcca tttatgctcc
     2521 tcctttttct tttattataa gaataaaaga aacacaatgc gactatttta tttggtagaa
     2581 ctgtgaggat agaaaagcag aagtattgct tgaatttttg ttttactttt tgattttaaa
     2641 tttagttgac taacggttgc tgttctggta agctagtact gttgtaattg tggactccac
     2701 agctacaacc gctcagaaca agtaataagc acagcatatg ctgtcacttc ggatggcgag
     2761 tctaagtata tcagaaggag gtgctttata gcatgtacgc aattattaaa actggtggta
     2821 aacaagtaaa agttgaagtg ggacaagcaa tctatgttga aaaacttgac gttgaagcag
     2881 gagaaaaagt tgtttttgac gaagttatct tagtaggcgg agaatctaca aaagtaggcg
     2941 ctccaactgt tgcaggtgca actgttgaag gtactgttga aaaacacggc aaacaaaaga
     3001 aagtcgttac tttcaaatac aaacctaaaa aacattcaca ccgtaaacaa ggtcatcgtc
     3061 aaccatatac aaaagtggtt atcgacgcga tcaacgctta atagcgtttg agaaagaagg
     3121 cctatatatg atcaaaggaa catttaagcg aaatgacgca ggtcagatcg tttcattcac
     3181 tttgaccggg catgctgatg ctggtcctta tggaagtgat atcgtttgtg cgggtgtttc
     3241 tgcgttagcg atcagcactg tcaacgggat tgcctcatta gccggttttg agccgattgt
     3301 tgaaatgaat gaagaagaag gcggttatct ttataccgaa gtcacttcag gaatgacaca
     3361 agaacaaaac aatattgctg agattctatt agaaaatctt ttattaggat tacaatcgat
     3421 agaagctgaa aactcagaat atatccaaat taaaacaatt aatgataaat aggaggtgca
     3481 gaccatgttg ttatctatga atctacaatt atttgcccac aaaaaaggtg gcggttctac
     3541 atctaacggc cgtgactcag aatcaaaacg tctaggtgct aaacgtgctg atggacaaac
     3601 agttacaggt ggatcaattc tttaccgtca acgcggtaca aaaatctatc caggtgtaaa
     3661 cgtgggtatc ggtggagacg acactttatt tgcaaaagtt gatggcgtag tacgtttcga
     3721 acgtaaaggc cgtaacaaaa aacaagtgtc tgtttatcca gtagctcaag aagcttaatt
     3781 gaatcttatg attaaagagg ttgtgacaag acttttgtca cagccttttt tgtttataaa
     3841 aataaaagtt gtagtggagg ctacgaagtt tactttgtct ttttacactt aatatttttt
     3901 tgccaatttg gaatcaggat gttttgataa ctatcgtttt caattgatgg aagctatatt
     3961 gtttgctatc agtgaaataa ttatatggta aacagtgttc tttttcttgt ttccattata
     4021 aaaatcgtat cgggattttt cgttctttta aattgaacat ttctatctat ccgcgtgtta
     4081 tcaacaaaac aaaatcaggt aaatagttga cgaaacagaa ataaatacat agtattatgt
     4141 tttgaagcct aagtacaata gtctatagaa aaggagcgat accattggca aaacgcattg
     4201 accgaattta tatgtatgtc aaagaaaaaa ccgctcattt aacaaaggca gaatatgatc
     4261 aaggagtaac aacacaggaa attgctgaag tgttagggat tcagcgaaca aatagcagta
     4321 aagatctgaa tcagctagtg cgcgaaggaa aactgataaa gacagacgga cgaccagtac
     4381 gttacatata tcaaacactc gttacgcacg taaagccagt tacaaaacat gtagtcagtt
     4441 acaaagaaac gccggtctct gaagaaaaaa atacactcca aatagatact aaggatatct
     4501 ttgcaaaaat cattggagct aacggcagca tgaaaaattc agtggaacag gcaaaagctg
     4561 cgattttgta tccgccaaga ggattaaatt gtttgattac aggacctacc ggatcaggaa
     4621 agacttattt tgcacacgcg atgtatcatt ttgcaaaagc gcatcatgta atagatgagg
     4681 aaacggaact gattgttttc aattgtgctg attatgccaa taaccctgag cttttgatga
     4741 gtcatttatt tgggtacgca aaaggtgctt ttacaggcgc agatgaagaa aaaatgggga
     4801 ttatcgatca agcggatggc ggtatgctgt ttttggatga gattcaccgg ttgccgcctg
     4861 aaggtcaaga aatgatcttt tactttatgg atcatggaac ttacagtcgt cttggggaaa
     4921 caacgaaatc ccatgaagca aatgtacgga tcgtaggtgc gaccacagaa gacccaggtt
     4981 cttctctatt agaaactttt gttcggcgta tcccaatcaa tatcaaactt ccggcttttg
     5041 aaaaacgtcc tgcaaatgag aaagtcgatt tagtaaaaat catgattgcc cacgaagcaa
     5101 accgcacgca gcgaaagatt tctttgacag aagatgtagc aaaagcattg atcggcagtg
     5161 tgacctatgg aaatatagga caattgaagt caaatatcca gttagtatgt gcacgaggat
     5221 ttctaaatca tatgcaatca cccgaaatct cgatcacaat cgatgatttg acagaaggga
     5281 ttcgtagcgg attgattcaa ttagcaagca accgcagtgc aatggcagag ctttctaaaa
     5341 tattggagcc aaagataact gtagcaccaa atgatacgat catgaaaatc caatcggact
     5401 cctatgagtt accttataat ctatatgata tcatcggaga taaagcagct ttactgaagt
     5461 cggatggttt ggatcaagaa gcaatcaatc atttcatttc tacagatatc aacatccatc
     5521 tgaagtcttt ctataaagac catggttttt cttttaatgc agacagcaaa ttagcagaat
     5581 ttgtagatcc gaaaatcata gaagtgacca atcagattta tgtaatggtc aaaagatctt
     5641 taccatatga gtttcagcaa aactttattt atgctatgag cttgcatatc agttcttttt
     5701 tgaaaaaaat caacttagga gaagaacggc atacgaatga taacatccgt gagatggctt
     5761 ttgattttcc agatgaatat gctgttgcga aagaagtgcg tagttatatc gaaacgtatt
     5821 ttcaagtgga gattccagaa agtgaagact attacttgac cgttctactc gtctcgttac
     5881 gggcaaatca agcaagcggc agaatcggtg tagtcgttgc agctcacgga aacagcacag
     5941 caagcagtat ggtccaagtt gtccaacagt tgttagatgc agagaacgta cgagcagtag
     6001 acatgccttt ggatatggat ccgaaaacag cattggaccg aattgaacgc aatgtacaag
     6061 aagtagatga aggaagcggg gtcatactct tagtagatat ggggtccctt gcgtcgttta
     6121 attcgcaaat tcaaagagac actggaattc cagtacgtac tgttgatatg gtaacaactt
     6181 ctctagtttt ggaaactgta cgaaaggtct cagtattagg gacagatcta gatatgttgc
     6241 atgaatcatt gaaaaatttt cgaggttacg cagaaatatc ttctgtagaa tctgttcagg
     6301 aagcaaaacc tattcataag aaaaaagctg ttttggctat ctgcgcatct ggcgaaggta
     6361 cagcacaaag aatcaaagaa ttgatcgaac gagcggtaaa caagcgtcaa gaaacagaat
     6421 tagaagttct agctttgtcg attgtggaag tgaaagaaga actgccagtt atccaaaaag
     6481 aatatcagat catagctact actgggatta ctgatttgaa tatccaagcc ccatttattc
     6541 ctttagaacg ttttattgat caaaatatcg aagtcatttt ggatcagttg ctgatggaat
     6601 cagaattgga ggaaactgaa ttcatctccc tagatgaaga gtcagctaaa aatacgtgtg
     6661 ttgagtttat ctctgataac ttcatcttta ttaatggtag taagttaatc gatccaatgt
     6721 ggcaattttc tacacaaatc tctcagacga cgggaatcgg cgatgaagaa tatggattca
     6781 aaatcaacct agtgatgcac acagcaggaa tgatcgaacg tattattcgg aatgaaccat
     6841 tgactgtgga agaaaacgaa ttgacaaata caacgaatga tcctctttat agccaattgg
     6901 cagcctcagt tgttctgttg gaagatcaga taaaagtaaa agtgccgata gaagaaatgt
     6961 attatctgct acgtctagtc cataatcagt tagataaaaa agaatacact gtgccatgaa
     7021 tacacaaaaa cgatacactt ttatagtgta tcgtttttgt gtattggtta agaatccttt
     7081 aaattcaatt agtgtatcgt aaaaaagttg gcacgctttt tgcttatagt agtgtgtgct
     7141 aggaggtggt tgttcacaca gaaatgatag gggtttctgt gaagtagtga ggaagaagga
     7201 gttttgcaga tggatattcg attggcacgt atagatgatc gtttaatcca cgggcaagtc
     7261 gcaacagttt ggtcgaagca aacaggtgtt gaacgtatca ttgtgatcag cgatgtagtc
     7321 gctcaagata aattgagaaa gtttctacta aaagaagctg caccgccggg gatcacagca
     7381 aatgtaataa ccgtgcagaa aatgattcaa atctatcata gtggattatt atcacgagca
     7441 aaagtgatgc ttttattcac caatccccaa gatgttgaag cggttgtccg ctgcggtgta
     7501 cagttgaagt cgttgaatat cggcggtatg agttttactg aaggcaaatc aatgattacc
     7561 aattttgttt cagtaaatca acaagatatt gattcctttc gttacttgaa cagtcaagaa
     7621 gtggagttag agattcgaaa agtacctgct gacaggaaaa tccttttgat ggaactacta
     7681 gcgaaaatca aaaaggtgta gcaagaatgg ttgttggaaa aggtcaaata attctgttgt
     7741 aatggttagc aattattaat atttttgaat ttgttacaac aaaagtaaat aggaggtata
     7801 aatatggtag ggattatcct agcaagtcat ggacaatttg ctgaaggaat cttacaatcc
     7861 ggttcaatga tctttggcga acaagaaaac gtcaaagccg tgattttgaa accaagtgaa
     7921 ggtccagatg acttgagagc caaattggaa gaagcggttg cttcttttga taatcaagac
     7981 gaagtgttat tcttagttga tttatggggc ggaacaccgt tcaatcaatc aaacacatta
     8041 ttcgaagagc acaaagataa atgggcaatc gtcagcggtt tgaatttacc aatgttgatt
     8101 gaagcctacg catcacgctt ttcaatggaa tctgctcatg aaattgcagc acacatcatt
     8161 gaaacagcaa aagacggtgt aaaagtaaaa ccggaagaat tagaaccggc agaagcaccg
     8221 aaagcagcag ttgaagacgc acaacctaaa ggagcacttc cagaaggaac tgtggtcgga
     8281 gacggaaaaa tcaaatatgt attagcacgt gttgactcac gtctgttaca tggacaagtt
     8341 gcgacagctt ggacaaaagc agtgcaacca aaccgtatta tcgttgtttc agatgcagta
     8401 tcaaaggatg atctgcgtaa gagactaatc gaacaagctg ctcctccagg tgtaaaagca
     8461 aatgttattc ctattagcaa aatgatcgaa gtagcaaaag accctcgttt tggaaatacc
     8521 aaagcattgc ttttatttga aaatcctgaa gacgtattga cagctgttga aggtggcgta
     8581 gacatcaaag aattaaatgt tgggtcaatg gcgcattcgg tcggtaaagt cgttgtaagt
     8641 aaagtattgt ctatgggaca agaagacgtt gaagcgtttg agaagcttga acaaaaaggt
     8701 gtgaaattcg atgtacgtaa agtacctaat gattcacgcg ataacatgga tgatattttg
     8761 aaaaaagcaa aagctgaatt agcaaaagcg taataaataa tattaaaatt tataggaggc
     8821 ttatcatgtc tattatttca ataattttag tcgtattagt tgcctttcta gctggtatgg
     8881 aaggtattct tgatgaattt caatttcatc aaccactagt ggcatgtaca ttgatcggtt
     8941 tagttaccgg taacctcgaa gctggtatcg tacttggcgg tacgcttcag atgatcgctt
     9001 taggttgggc aaatatcggt gccgctgtag caccagatgc tgcattagca tcagtagcat
     9061 cagcaattat tttagtttta ggtggacaag gtgttaaagg tgttccttct gcgatcgcga
     9121 ttgccgttcc tttggctgtt gcagggttgt tcttaacaat gatcgtacgt acaatcgctg
     9181 ttccaatcgt tcatttgatg gatgcggctg ctgaagaagg aaacatccgt aaggttgaaa
     9241 tgtggcacat cattgctgta tgtttacaag gggttcgtat cgcggtccca gcagcagcgt
     9301 tgctatttat cccagcacaa actgtgcaat cattccttga atcaatgcca gcatggttaa
     9361 cagatggtat ggctatcggt gggggaatgg tcgtagctgt aggttacgct ttggtaatca
     9421 acatgatggc tacaaaagaa gtatggccat tcttcgttat tggtttcgtt gtagctgcaa
     9481 tttctcaatt aacattgatt gctctaggtg ctttaggtgt tgcattagct cttatttact
     9541 tgaacctttc taaaatgggt ggttcatcaa ataatggagg cggaagcaac tctggtgacc
     9601 ctttaggcga cattttgaat gactattaat cttgaaggag gaaaataaaa tggcagaaga
     9661 aaaaatcaaa ttatcaaaaa aagatcgttt agctgttgcg tggcgctcta ccttcattca
     9721 aggttcatgg aactacgaac gtatgcaaaa cggtggctgg gcattttcta tgattccagc
     9781 aatcaaaaaa ttatacaaaa caaaagaaga acgttctgca gcattaaaac gtcacttgga
     9841 attctttaat acacacccat acattgcttc tccaatttta ggggttacat tggctctgga
     9901 agaagaacgt gcgaatggcg cgcctgttga tgatgtagcg attcaagggg ttaaagtagg
     9961 tatgatgggt cctttagctg gtgttggtga cccagtgttc tggtttacag tacgtccaat
    10021 gctaggtgca ctaggtgctt cattagctat gggaggaaat atccttggac caatcatctt
    10081 cttcttaggt tggaacatta ttcgttgggc attcatgtgg tatacacaag aatttggcta
    10141 caaagctggt tctaaaatta ctgatgacct ttctggcgga ttgttacaag acgtaacaaa
    10201 aggtgcttca attttaggga tgttcgtatt agctgccctt gtagaaagat gggtatcaat
    10261 caaattctta ccagttgtat caactgtaaa attagataaa ggtgcttaca tcgaatggga
    10321 taaattgcct tcaggtggtg aaggaatcaa gagtgcattt gaacaagtta ataatggctt
    10381 agccctttct cctgaaaagg taacaacatt acaagacaac ttagaccaat tgattcctgg
    10441 tttagctgca ttaggattaa cattgttatg tatgtggttg cttaagaaaa aggtttctcc
    10501 aatcgttatc atccttggtt tattcgtagt cggtgttgtt ggacacgtca tcggactttt
    10561 ataatccata aaaatatagc tcgggcgttg tcccgagctt tttttatata gtatactgtt
    10621 cataaacaaa tatctggagg gagaaaatgg ttcaatcaat caatacaaaa gtagacttgg
    10681 taattgatgc tacttcgttt acgggtctga cagattacgg gcaaatcatg ataggtgata
    10741 atggttttga gttttaccat gcgcgagata gacgcaaata tatccaaatt ccatgggaag
    10801 aagtggatta tgtactagca tcagtcatgc taaagggaaa atggattcct cgatatgcca
    10861 ttcaaacgaa aaaaaatggt acatacacat tttcttccaa agaacctaaa aaagtgcttc
    10921 gtgcgatcag agaatatgta gatgaagata gaatggtaca atcattgagt ttctttgatg
    10981 tagtgaaacg tggtattcgt agtatgttta aacgcaagta aaaaataaag aaaacttcta
    11041 agaggaggtt ttctttattt ttttactttg cagaagaaaa tatgaaatag caaaaccaat
    11101 tatttgtccc ctttaaatcc catatgctaa aattgattag caaagaagga ggttatatta
    11161 tgagttggtt atggacattg attgttggag ccgtgatcgg agcaattgca ggtgcgatca
    11221 ccagtaaagg aaattcgatg ggctgtgtaa cgaatatcat tgctggacta gtcggttcaa
    11281 gtattggaca agcgattttc ggcgaatggg gaccaaaagc tgcaggtatg gcacttattc
    11341 catctatttt aggcgcagtg attttggtag cagttgtctc atttttcttt ggaaaacgat
    11401 cataagtttt tacaaaatag taaactaaaa ggagttgcag cgagactgtg actccttttt
    11461 ggctttattt atttaaaata aaaagaagtt ttccggaaat ttttatttaa ctgtcgtgta
    11521 cgaagaaaag tttgctataa tggaaaaaag cgtgcaaaga aagagggagt ttcattatga
    11581 tgctacgtgt agaaaaattg cgcaaaaaaa tgcaagaaga aaatttggat tcatttttgg
    11641 ttaccagtcc gtacaatctt cgttacttaa cgaactttac aggtacgaca ggattagctg
    11701 ttattacttt ggaaaaagca ttttttatta ctgattttcg ttataccgag caggcggcag
    11761 cgcaggctca agggtttgaa attattaaaa atgtcggacc tatttttgat gaggtagcaa
    11821 acttagtacg caaagaagaa ctgcactcat tagcttttga ggaaacaaca gtctcatttt
    11881 tagaatattc ggttttagaa gaaatcatcg atgcggaatt agtacctgtt tcacagatga
    11941 tcgaagaact cagagaagtg aaagatgaag aagaaatcgc tattatcgaa aaagcatgtc
    12001 atattgcgga tatggcatat gatcacatcc tgaaaatgat ccaaccgggc atgactgaga
    12061 ttgaagtcgc aaatcaacta gacttttata tgcgttctct tggcgcttca ggtgtttctt
    12121 ttgatacgat cgttgctagc ggattgcgtt cggctatgcc gcatggtgta gcaagcaaaa
    12181 aaatcatcga acaaggtgac ttgatcacat tggactttgg ttgctattac gaagggtacg
    12241 tttccgatat gacacgtaca ttcgctattg gtgatcctgg ggaaaaatta aaagaaattt
    12301 atcaaatagt cttagatgct caattagcag ttattgatgc tgcaaaacca ggaatgactg
    12361 gtgtccaatt agatgcagtt gctagagatt atatttcaaa acatggttac ggggaagcat
    12421 ttggacattc tacaggacat gggatcggtt tagagatcca cgaaggacca aacgtttctt
    12481 ctcgtgcaga aaagcaattt gtaccaggaa atgtgatcac tgatgaacca gggatctact
    12541 taccaggaat cggtggagta cgtattgagg atgacttgtt gatcacagaa gatggaaatc
    12601 gtgtgttgac tcgttcacca aaagaattga tcatcttata gtttcatcaa tagaagaagt
    12661 aagcgtaaaa atcagtgttc acagactttg ttatagaaaa tcactcgatt taatgataca
    12721 ctatagaaga acttttaagc tgtaagagat aataaagttg ggtgttttct ccaattcata
    12781 gcagtttgca gctgatcggc ggcagttttc ttaccacgtg atcactatgc cgcatcatag
    12841 agaattcgga tgtatctcgt gcttaggcaa agggaggaat aaataaatgg cagatgaaaa
    12901 aaatttgatt ttaaacgcaa accaagattt aggagagatc gtgatcgctc ctgaagtcat
    12961 cgaagtaatt atcgggattg cagcttctaa agtagaaggc gtatacggaa tgcgcggaac
    13021 ttttgccagc aatgtcactg aattattagg acgtgcagct cacggaaaag gtgtttactt
    13081 agcaaacgag gaagatggct tgaaagtaga tctttactgt tatctagaat atggtgtttc
    13141 agttcctaaa gtagcaatgg aaatgcagga acgggtcagc cagcaagtcc ttttcatgac
    13201 ggatatcgat ttatctgaag tcaatatcca tgtagtagct gttgttcctg aaaaattaga
    13261 acaaccaaca atcgaagaac tttttgaaga cgaagaggaa gagaatgaat aaagaattat
    13321 caagacacga gattcgggaa atggcattgc aggctttatt tccattagac ttcaatgcag
    13381 acctaacaaa agaagatgcg attttcaacg caatcgaatt agaccaccgc gacatgatca
    13441 acgaagatga atcagagttt gttcctgttt atcttgatac gctagttggc ggtgtctgtg
    13501 caaaaaaaga tgaattggat aaagtgattg aaaaacattt aaaaaacaat tggcacatca
    13561 atcggatctc aaaaatggat ttggtgatct tgcgtatcgc gatttttgaa atgacttatg
    13621 tgacagatgt accagcggct gttgctttaa atgaagcaat cgagttggca aaaacattta
    13681 gtgatgaccg ttcacgtaaa ttcgtcaacg gtgttttatc caatgtattg aaagaattag
    13741 aagctggggc gtaagtccta gcttttttct atgaatcatc ataaacctgt tataaatcgg
    13801 gaaaattcta tggtagaatg aaaacacaaa aaatatctgg gagtggttag atggcagaat
    13861 tgattaacgg aaaagaatta gctgaaaaaa tgcaggcaga gattgctgaa aaaatccaaa
    13921 aattgaaaga taatggtatc catcccggat tagtcgtttt attggtaggg gaaaatccag
    13981 ccagccaaat ctatgtaaga aataaagaac gtgtagcaaa agaaatcgga atccattcat
    14041 tggtagagcg ttatcctgag acgatttctg aagacgaact acttgcagaa attgaaaaat
    14101 acaatcaaga tcctagtttt catggtatat tggtccaatt accattgcca aagcatatag
    14161 atgaagaaaa gatcctgtta gctatccgcc ctgaaaaaga cgtagacggt tttcatccga
    14221 tgaatttagg cagactcctt gcgggtaaac cggacaagat tccttgtact ccatacggca
    14281 tcatgaaaat gtttgaagct tattctatcg atccggcggg taaacgagca gtagttatcg
    14341 gacgaagcaa tatcgtcggt aaaccgatgg ctcagctact cttgatggca gatgcaactg
    14401 ttactgttgc tcattctaaa accgaaaatc tggctgaact ggcacaagaa gcagatattt
    14461 tagtcgtagc aataggacgt ggacattttg tcacaaaaga attcgtaaaa ccaggtgctg
    14521 tagtgataga tgtagggatg aatcgtgatg ctaacggtaa gctgatcggt gatgtgaaat
    14581 ttgatgaagt ggagcctatt gcaggctgga tcactcctgt gccaaagggt gtagggccaa
    14641 tgactatcac gatgctgatg tatcaaaccg taaaatctgc cgaagctttt ggagcaggtg
    14701 aatagatggc acaagaatat ttaactgtca ctacattaac gaagtatttg aaaagaaaat
    14761 ttgatgccga tccttattta gaacgcgtat atttaacagg cgaaatatcg aatttccgtt
    14821 tacgtgccaa tgcccatcaa tatttcagct taaaagatga ccatgcaaaa atatctgcta
    14881 ttatgttcaa aggagcattc caaaaattac gattccagcc aaaagaagga atgaaagtcc
    14941 ttgtcgttgg gcgtatttca ttatatgaag cgggaggttc ttatcaaatc tatgtagagc
    15001 acatggagcc tgatggaata ggtgctttat atcaagaatt ggaagaacga aaagaaaagc
    15061 tgatgaagga aggccttttt caagggccta aaaaaatgct gccacgtttc ccaaagcgaa
    15121 tcgcggtcct taccagtcca agcggagcgg ttatccgcga tatcatcacg acggtgaaaa
    15181 gaagatatcc aattgtccaa ttggttttgt tcccaacagt tgtacaagga aaccaagcag
    15241 cggacaatgt tgtcaggaat atccagagag tagaagagat gggcaatttc gatacaatga
    15301 tcatcggtcg tgggggtggt tcgattgagg atttatggcc gtttaatgaa gagcgggttg
    15361 ctcgggcgat ttatcaagct aatacgccgg tgatttcatc tgtaggacat gaaacagata
    15421 ccacgatcgc tgatttagta gcagatgttc gggctgccac tccaacagca gctgcagaat
    15481 tagctgttcc tgttttgtca gaagagttga tgcgcattca agaacggcaa gctagattgc
    15541 agcaggcgtt tgtacgccag atccagcgca aacaagaacg gttcgagcgt gctcgacgtt
    15601 cctatgtatt tcggcagcct gaaagattgt atgaaggtca gaccatcaaa ttagaccagt
    15661 tgaaacagcg attatatcaa gctacacagc aaatttatca tgcgaaagaa aaacaaacga
    15721 tcgcattaag ccaccaattg gaacaagtgg ctccgcttta tcgagttaaa gcagctcgcc
    15781 aagaaactgc ttatctagaa aaacgcttag aagagaaaat ccaacaatat atgaaacaac
    15841 agcagcagcg ttttgctcaa gctgttcaat ctcttgattt gttgagtcca ttgaaaatca
    15901 tgggaagagg ctacagctat acgacgttaa atgatcaagt agtaaaaagt gtcaccgaga
    15961 ttcatcaagg ggatttgctg acgatccatt atcaagatgg aatcgttaaa ggacaagtac
    16021 aagagataga caaaggagaa taaaaatatg gaaaatccaa cttttgaaga gtcactgcaa
    16081 gaattagaaa aaattgtcat gcagttagaa caaggagacg ttccattaga atcagctcta
    16141 gattcgttta aacgaggaat ggaattaagc aaacattgcc aagatacatt agctaaagca
    16201 gaagagacct tgacgaaaat gatgacagaa tcaaatgaag aagttgattt tgacggaaac
    16261 gaggaaagct gagatgagat taactgactt ttcagctatt catctacctg ctgtagaaaa
    16321 agaaatcttg tcttttttag atgagcatac aacggataga ggactctatg atgcgatgag
    16381 ttattcagtg aaagcaggcg ggaaaagaat acgtccctta ttacttttaa cggccgttgc
    16441 ttcctttgat gagcctattg atgttcccgt ctatcaagta gcagctgctt tagaaatggt
    16501 gcatacgtat tccttgatcc acgatgattt gcctgcgatg gacaatgatg atcttcgtag
    16561 aggaaaacct accaatcata aagtattcgg cgaagcatta gctatattgg ccggagatgg
    16621 tttgcttaca ggagcattcc agctaatcag tatggcacac ttaggtaatt ctcctaagct
    16681 tctgctgctt cagcaattag ctgtttgtgc gggatcacaa ggtatggttg ctgggcaagc
    16741 tgcagatatc gagggcgaat cgaaaaagct ttctttagaa gaattagcat ttattcatga
    16801 aagaaagaca gggcatttga ttcgttatgc cttgctggct ggtggcatat tagcgaagca
    16861 acctgaagaa atcttattgc tacttcaacg attagccgaa catttaggtt tagcttttca
    16921 gataagagat gatctactcg atgtgattgg tacaacgaaa acattaggga aaacagctgg
    16981 taaagatgaa cgtatggaaa aaaatactta cccgcgttta ttaggcctag aaaagacacg
    17041 tgaggcgttg gagattgagc tgttatccgc caacaagatc atagacaaat tagaagagga
    17101 cgttttttct tttgatggcg ggctgatgcg ccagatgatc aagcaatttg atgtggagaa
    17161 atgagggaaa gaatggaaaa agaacgcgta gatgtattag cggtcaaaca aggattattt
    17221 gaaacaagag aacaagccaa acgttcagtg atggctggtt tgatttacaa cgaaaaaaac
    17281 gagcggtttg ataaaccggg agaaaaaatt ccagtgagta gtgaattaag agtcaaaggt
    17341 aaaaaacttc cttatgtttc tcgaggggga ttaaaattag aaaaagcact caaagcattt
    17401 gatctatctg ttgaaggaaa aacattgtta gatattggtg catccactgg cggatttact
    17461 gatgcagctt tgcaaaatgg cgcaaaaatg agttatgctt tagacgttgg ctacaatcag
    17521 ctagcttgga aaataagaca agatcctaga gtcgtcgtaa tggaaagagt gaattttcgt
    17581 tatgcaaaac ccgaagactt taccttagga attccagaag ttgctgttat cgatgtttct
    17641 tttatttcac taaaactgat gctgcctcca ttacatgcta ttttaaaaga agagggagaa
    17701 gtgattgctt taatcaaacc ccaattcgaa gcaggcagag aagctgtagg gaaaaacgga
    17761 attgttcgtg accctcagac ccatcaaaaa gttttagaag acattttgtc atttgcggca
    17821 caaggtggat atgatgtgct ggaattaagt tattctccga tcacgggggg agaaggaaat
    17881 atcgagttct tggctcactt gaggaaagta cccgaaagcg gaacaatcaa ttcagctatt
    17941 aatatggcag aagtggtttc aaatgcacac gaacagtttg accataaata aattgtaact
    18001 gtgaaaatca agtgagaagt atgcggattc ttctttatta taccaaggaa ataagctatc
    18061 attgattaag aagaatatgc taaggagcga agacgtaatg agaaaaaaag atcggcatcg
    18121 tttgattact cgattgttga gtgagcaaga tatccgaaag caggaagaat ttgttgaaat
    18181 cctgaaaagc aaaggaatat ctgttactca agctacgatt tccagagata taaaggaact
    18241 gaaactgatc aaggtaccgg cttcagatgg cggctaccgt tatagcatac cagctgaaac
    18301 aaacgaagat gtaaatacta aattggaaaa actattaaaa gacgctttcg tggcagttga
    18361 ccaaatggaa aaatttgtta ttttgaaaac attgccggga aatgcttcgg cagcagcaaa
    18421 tttgattgat aaacgttaca agaaagaatt attttcgatt atcaatgatg acgataatgt
    18481 tttgatgatt acacgaactg aagaccaggc agttttattg aagaaagact tcctgcatta
    18541 cctttaagtt gaggagcgaa tgtgatgcta caagaaatca gtattactaa ctttgcgatt
    18601 attccggaat tgcggctttc ttttcacgaa ggaatgaccg cgctgaccgg agagactggt
    18661 gcgggtaaat ccattattat cgatgcttta ggtctgttag caggaggaag aggttcaagc
    18721 gattatatac gtcaaggagc agaaaaatgt gtgcttgaag ggttgtttga actaccaaaa
    18781 caagaaggat tctctgaact gatggttgaa ttagggatcg aaacagatga ggacaatctg
    18841 attgttcgac gggatatgtc cttaactgga aaaaatgttt gtcgagtgaa tggtcatatt
    18901 atcacattag ctaatctgag aagaatcggc agttatttag tagatatcca aggacaaaac
    18961 gaacatcagg aactgctcca gcctgaatca catcttgctt tattggatcg ttttggagat
    19021 gtagctttcc aacagaagaa aaagagttat cagcaagaat atatctctta tcgtgagctg
    19081 gaaaaaaggg tacgaaaagt gcagcaaaat gaaaaatcct atgttcagag aatagatatg
    19141 ctccatttcc agcaagaaga aatagctgct gctgatttgc aagtcggaga agaagagaaa
    19201 ttaaaagaag aaagagaaaa acttagcaat tatcaaaaaa ttgctgatgg attagctgca
    19261 ggttacggag cactgacgaa taatgaacaa aacagtgtag atggtgttgg cttggccgtt
    19321 tcagaactgc aagggatcgc ccatctagat gtggagtatg aagcaattta tgaaaatatc
    19381 caaagtgctt actatttgct gcaagatgca atcggcgata tgagtcgtca gattgattta
    19441 ttggagttag atgagagtcg tttagaagaa gttacgcaac gtttagaatt aatccgacag
    19501 ttgaaacgta aatacggcga atccattgag tcaatattgg cttactatga cgaaatcacc
    19561 gaagagctag cttcttctga tttttcagaa ggtcaattgg acaaaatgaa agaggaacta
    19621 gagcaaaaag agcttctact tcagcaacaa gccgcagatc ttcatgaagc gcgtaaaaaa
    19681 atcgcaaaag aattggaaaa atccattctg catgaattga aaagtctgta tatggaaaac
    19741 actgagtttg aggtacgttt cttaaaagaa gaaaatagac aattgaatca tgatggattt
    19801 gaccaaatcg aattttacat tacgaccaat ccaggagagc cactgaagcc tttggtgaaa
    19861 gtcgcttctg gtggcgaact gtctcggatg ttgcttgctt tgaaaactat tttttcttct
    19921 gagcaaggtg tgacaagtat tatttttgat gaagtggata caggtgtgag cggaagagtt
    19981 gcacaggcca tcgcagataa aatttctcaa atatcgaaat actctcaagt tttgtgcatc
    20041 actcatctgc ctcaagttgc tgcagtcgcc gactatcaat attatattgt gaaagcagtg
    20101 attgatggac gtacgcaaac ttctgtttca gagttgaaaa caaaagaaag agaagaagaa
    20161 attgcacgta tgctggctgg aagtgagatt acaaaactca cactcgaaca tgcaaaagaa
    20221 ctattgaaac tcgcaaaaag gacctgagcc acctcgcagt ggttcaggtc cttttcagca
    20281 gctgatagct atattttatt tttacatgac actcattaca gcagtcaata caacagatcc
    20341 aatcgttaaa atcagcattg tccaaatgac aaattttgtt actttagcaa aagtacttgt
    20401 ttgttttttt tcacgcattg ttttcaacct cgtcattaat tagttgattc aagtgtacaa
    20461 tcttttttcc atttccacaa gagggctttt ttataaaaac ttcccttttt ttaaataata
    20521 ggtagttacg atacaaataa ataatgttaa tagaactagc catataaagg catcatccct
    20581 ttcagcaata ggtaacttaa tgttcattcc atagatactg ccgacgattg tcggtatagt
    20641 taaaataatc gttaaagaag ttaaaatttt cataatatta ttcagattat tcgatacgat
    20701 tgctgaaaaa gtctgattca tttgttctac tagttttaat tgaatgcgag aagtagtcat
    20761 tgcctgctct gtttccacca atacatcacg taaatgattt ttcaaagcat gattttcttg
    20821 gaaaagttta ttttgcgcta gttctttcaa tgcatctaaa ttagcagaag tcgcttctct
    20881 gaaataaacg aggctttttt ggatatccat gatttgataa agttgtttgt tttcagtgga
    20941 tacttgaagt ttggcttcca aagtatcgca ttgagattga agcgtagcta aataactgtt
    21001 gtaagagagt gtgacctgcc aaatgaattg aaagaagata ttgagattag gcgaaatatc
    21061 actttttgga aaggtctggc taaagacttt ctttaaaaac agtggttcat gattcgtaat
    21121 tgtgataagc ttctttttgg gcgtcaaaat aatagagagc gggtatgtat ccacttgaaa
    21181 ataaccgcta ggacttgttt tagcaaaagg atattgtaac aataataacg ctggctttgt
    21241 caactttgat tgattgaatt cttctgtacg ggaattttct gcatcgtcca atatggaagt
    21301 gatgtaatct gacggaattt caaattgatg tgtcagctga tgaatctctt cttgattagg
    21361 acgttctaca caataccaga tcgtttggtc agtttccttt ccggtattac gcagtttctc
    21421 attatctaat gtaaaatatt tgatcatgat tttcctcctg ttgcctgttt cttttattat
    21481 aacggatagg gaattggaat acatggaatg tgtttgtaag atatagaata taactgcata
    21541 aatttaaata aaatgaaaca gttgtgtaaa gtatatacag tatacttgaa gcaacgataa
    21601 aaaaggagtt tacgttcatg ggcacattca caccacttat cttgttttgg tatatttacc
    21661 cggtaatcgt tttgtttggt tgccaatttc ttgtaaaatt attttcgtta aatcgtcgtt
    21721 ttaaaataaa agcccctgat ttggcgattc cttttttgtg gggcggtatg catgcattgt
    21781 ctagaaatac agggacaatt tctatcttac cgtttttctt tatttctgtt ttgctaatgg
    21841 gaatttgtat tgctgttttc caagcatatt attatgaaga aattatttat ccgcgttatt
    21901 tcaagatgac ttggcgtttg gtttttctgt tgacaatcgt cttgtacgct gtcttgatca
    21961 ttgtgaacat actttcttac ctatgaaaaa aatagaaaag ctgtaaaaag tcttttaaaa
    22021 ggctttttgc agcttgattt ttaaaaaaat gtttatgaat aaaaaaagtt gttcccggat
    22081 ttcccagaaa aggtggtaga aagtggggga ttgtggtaga ctaattttag ttagtggagg
    22141 aatggggggc tttcaatgtt gatgggtgaa ttccaacata atattgatgc aaaaggacgt
    22201 ttaatcgtgc cctcaaaact tcgtgaagaa cttggcgaaa agtttgttct gacacgcgga
    22261 ttggatggct gtcttttcgg ctatccgatg agcgaatggg aaaatctgga agctaaactg
    22321 aacgaaatgc cgttggctaa aaaagacgca cgtacttttg tgcgattctt ctattcggct
    22381 gccactgaat gcgaacttga taaacaagga cgaatcaata tacccagcac gttaagaaat
    22441 tatgcagctt tgacaaaagg ttgcgtagtc atcggtgtat ctaaccgaat tgaaatttgg
    22501 gatgagacac gatggcaaga tttttctgca gcagcagaag agaactttga cgaaattgct
    22561 gaaagtatga ttgatttcgg actataagaa taggaagagg ctgactaatg actgaaacat
    22621 ttcaacacta tacagtcatg ttgaaagaaa cggtagatgg attgaacatc aaaccggatg
    22681 gcgtttatgt agactgtaca ctcggcggtg ccggacatag tgaatattta ttaagccaac
    22741 taagttctaa agggcatctg tatgcgtttg accaagatca aaaagcaatc gatcacgcca
    22801 agctgcgttt agcttcttat gtcgaacaag gtatggtcac atttatcaaa gcgaacttca
    22861 gagaattaga agaacgcttg agtgagcatg tagataaggt agacggcatt ttgtatgatc
    22921 taggggtttc ctctcctcag ctagatgaag cagaacgtgg atttagttat catcaagatg
    22981 caccgttgga tatgcgcatg gatcagtcag ctcctttatc cgcttatcac gtaattaatg
    23041 aatattccta ccatgaatta gtaaagattt tcttccgcta tggcgaggag aaattttcca
    23101 aacaaattgc tcgtgaaatc gaacgagtac gaaaaacaaa accaatccaa acaactggtg
    23161 agcttgtcga aatcattaaa caagtcatcc cagctcccgc aagaagaaaa ggcgggcatc
    23221 ctgcaaaaag aatttttcaa gctgtgcgga ttgcagtaaa tgacgaactc ggtgccgaag
    23281 aggcctcttt agaacaggca atccggttat tgaaagtcaa tggaagaatc agcgtaatca
    23341 cattccattc attggaagac agaattgtca aaagtatgtt taaagaatat agtacggtac
    23401 aagatctacc accgggactt cctgtcatac ctgacgaatt ccaaccagaa ctgaaagtca
    23461 ttactcgtaa accaatcctt ccttcagagg aagagctaac agaaaacaat cgatcacgca
    23521 gtgcaaagtt acgtattgca gaaaaaataa gaatgaaaga ggagtagaat gatggcagaa
    23581 ttaaagaaaa tgcaagatta cgaatatgat ttacctgaat tggtggatca gccggaacaa
    23641 caaatgcctg attcttcttc gaatcaagaa atcattcttc cacaatcacc ggcaaaacga
    23701 ttaaagcaga tttctgcttt ggaaaaaatt gctgtggcaa gcgtggcgat tgctattgtt
    23761 gctttatgtg ttttaacagt tatgctgcgc acaaatatca gcggagctga aaaagaaatc
    23821 acgtcaatac aaacagaaat caatgataaa aagcaagaaa aaacgagttt agaacaagaa
    23881 aaaaacgaac tttctcgtac tgaacggatt aaacaaatcg cagaagaaaa aggattgtct
    23941 attaatgacg acaatttaag gaaagtgaag taaatgagtc ttaaaaataa aattcggcgg
    24001 tttatggaaa agaaaaatct aaatccgatg aacaatcgca aaaaagtagg tatcatttta
    24061 tttgctacga gtattggaat gttcttttta tttgccgtcc gtttctcgta tatcgttatt
    24121 ggaggtcatg ttgctggtac ctcattagct gaaaaaacaa aacagttgta ccaaggaagt
    24181 gaagtagtaa aagcaaaaag aggaacgatt tatgatcgta acggtgtggc gctcgcagaa
    24241 gatgcctcct cttattcaat taaagcgata ctctcaaaaa catatacctc tggggataaa
    24301 aaactgtatg ttgaagaaaa gaattttgat aaaatagcag aaatcctgca taagaatctt
    24361 tctatagaca aaaaagatgc actgaatatt ttagaggacg gcgcaaaaaa agagctgtat
    24421 caagttgaat ttggtagcta tgggaaaaat atctcccaag aaaccaagca aaacatcgaa
    24481 gcggacatga aaaaagaagg tgtcgctggt ctttattttg tcgaccatca ggctcggatg
    24541 tatccaaatg gcgtcttttc ttctcatttt attggatacg cagttccaga caaagatgag
    24601 aatggcttag ttggtaaact aggtttggag tctgcttaca atgatatctt gagcggtaaa
    24661 gatggtaaga ttatttatca aaaagataat tttcaaaatc ctctgccagg tacagtggca
    24721 gaagaagaaa aagctgtcga cggacaagat atctacacga ctttagacag cagactacaa
    24781 agttatttag aaacactgat ggatcaagta aatgaagaat atcagccaga agaattgaca
    24841 gcagtcctga tgaaagcaaa aaccggagaa atcttggcaa tgggacaacg acctactttc
    24901 aatcctgaaa caatggaagg attgactgga aaagatgcta tctggcgtaa tttcttggta
    24961 caagatagtt atgaaccagg atcaaccatg aaagttttca ctactgcagc tgctatcgaa
    25021 gaaggagaat tcaacgagaa tgaaaccttc caatctggaa aaatccaagt agaagatgct
    25081 acgatcaatg accatgactt tggtgaaaaa ggtgtgctga ccatgcgtca agctctttca
    25141 tggtcaagta acgtcgggat ggtcatactg gaacagcgat taggcggacg atggtataac
    25201 tatttacaaa aactgggatt tggacaaagt actcattctg gattagatga tgaagtaaac
    25261 ggtgctttac ctacattaaa tattgtcgac cgagcaatga gtgcttatgg acaagcggta
    25321 ggtgtgacga atttccaaat gatgaaagct ttcacttcta ttgcgaataa cggtacaatg
    25381 attcaacctc gatatatcag caaagtggtg gatccccaaa ctggagaaga gcgaacaact
    25441 caaacagaag ttctagggca acctttttca aaagaaacaa cagaaaaagt ccgtgaatat
    25501 atgagagatg ttgtcgaaag cgaaaactac ggtagtgctt atggtgtata tagtgtacct
    25561 ggatacaatg tatcggcaaa aaccggaaca gcccaaatcg cttctgatac agggggatat
    25621 caaacaggag atacagccta cctgtattcg attgtggaga tggttccttc tgaagatcca
    25681 gactatgtct tgtatctaac aatgaaacac cctaagacat atgatcgaat ggcattggct
    25741 aaaatcgcaa atccattgat gaaacgggca atggatttta aagaaactga agaagattca
    25801 gatacagaaa caaaaacaga aaaagtatcc gtagccgatt atcgtaatct tgaagcggac
    25861 gttgcagcag ctgatgctca aaaaagtgga ctgcaacctg tagtgatcgg taatggtaaa
    25921 aaagtacaaa aacaatctac tgccaacggt gatcagttga tctctggaga aaaactgatc
    25981 ctatatactg gtggtgataa gctgatgcct gacgtaacag gatggtcaaa agcagatatc
    26041 atgaagctgg ggaaaatcct cggtattgaa gttagttttg atggagatgg ttattgtgtc
    26101 aaacaagaac ttgctccata tgaaaaaatc actgaagaca aacttagttt tactttagaa
    26161 gaataagaag agaaatagga gagaaaagag catggaatgg acacaagcat tgattcccat
    26221 tgtcagcagt tgtgcaatga caattgccgc aatgccgcta tttattggtt attttcaaat
    26281 gaaaaagcaa ggtcaggcga ttcgtgagga gggaccaaag tggcacaata gcaaagccgg
    26341 aactcctaca atgggcggct tagtgtttct gatcggctct attttgacag gaatttgggt
    26401 tggagcttgg caaaaacagc taactcctac tcttttcatc ttgctttttg tattagcgtt
    26461 atacggagta atcggctttc ttgatgattt tataaaaata ttcaagaaaa gaaacatggg
    26521 actgaactcg aagcagaaat tgattggaca gattctaggg ggaatcatct tttatcttgt
    26581 ttaccgttct gaaggttatc caggtgtatt gaatttcttc ggactagatc tacctttagg
    26641 aatcatttat ggtattttcg ctctcttctg gcttgttgga ttttcaaatg cagtaaatct
    26701 gacagatggc atcgatggat tagttgctgg cttaggaagt atctcttttg cagcttacgg
    26761 tctaattgct tggcatcagc atcagtatga tgtattaatc atttgcttaa gtgttttagg
    26821 aggtttactg ggtttcttcg tctacaacag aaaacctgcc aagattttta tgggagatgt
    26881 cggttcatta gcgttgggcg gacttttagc ggcaatttct ataatgctaa accaagaatg
    26941 gacattgctt ttgattggtt tgatctatgt tatggaaaca gcaagtgtga tgcttcaagt
    27001 tacttccttt aagcttacag gaaaacgaat ttttaaaatg tctccaattc atcatcactt
    27061 tgaaatgtgc ggatggtcgg agtggaagat tgatacagtt ttttggttga tcagcattgt
    27121 gacttcgttg atcacattat ggatcgtttg gtaggagatg acgggaaatg aaaaaaatat
    27181 caacatatga aaataagaaa gtgcttgtct taggactcgc aaaaagcgga gtaagtgcgg
    27241 ccaaattatt acatgaattg ggcgcacttg tcaccgttaa tgatggaaaa ccttttgacg
    27301 aaaatccgga agcacaagag ctgctttctt tagggataaa agtaataaca ggtagtcatc
    27361 caatcgaatt gttggatgaa gaattttcat tgatggtcaa aaatccaggg attccctaca
    27421 ggcatccttt agtagcaaaa gcccaagaaa tgggtattcc ggtaattacg gaagttgagc
    27481 tagcttacga agtagcggaa tgcccaatta ttgggattac aggaacgaac ggaaaaacaa
    27541 cgacaacaac gatgacagga ctactactga atgcaggagc agatcaagga attgcacgtt
    27601 tagcgggtaa tattgggtat ccagcaagtg gtgtagcgca agaagcaaaa tcagaagata
    27661 aaattgtgat ggaattgtct agcttccaac tcatgggaat cacagatttc cgccctcata
    27721 tagcggtgat taccaatatc tatgaagcac atattgatta tcatggtaca agaaaagaat
    27781 atgtcaaagc taaatggaac cttcaaaaaa acatgacaga aaaagattat ctgatcctta
    27841 actggaatca gtcagaacta caagaattag cgcaaagaac aaaagcacgc gtactgcctt
    27901 tctcgacaaa agaagtgttg gaagacggtg tgtatgctga cgattactca atatactata
    27961 aaaaagaaaa aataatggaa atatctgaac ttggcgtacc aggtaagcat aatgtagaaa
    28021 atgcactagc tgctatttct gtagcaaaat tgtatggtat atcaaatgaa gctatacgag
    28081 aaacgttgca ttttttccat ggcgtaccac atcgtacgca atatgtagga gaaatccaag
    28141 gcagaaaatt ttataatgat tcaaaagcaa ccaatatttt agcgactaaa atggctctaa
    28201 gtggatttga aacgtctaag gtcgtgttat tggctggcgg attagacaga ggaaatacat
    28261 ttgatgaatt aattccttca ttaaaaggaa tcaaagcaat ggtcgtattt ggccaaacaa
    28321 aagaaaagtt gatggatgct ggaaaaaaag caggcatcga gacaatagta acagccgatt
    28381 ctgtagaaca agcagttcct ttagcattag aaaacagtac agatggtgat gtagttttat
    28441 tatcaccagc gaatgccagc tgggatcaat acccaaattt tgaaactaga ggaaacagat
    28501 tcatggaagc tgtgaaccga ttaaaataga aaaagtagaa gcgagcgata aaaaatgaaa
    28561 attttggtta caggcggagg aacaggggga catatttatc cagctcttgc ctttgtcaat
    28621 tatgtaaaga ctcaagaacc gaatgctgaa ttcatgtatg tgggagcaaa acgaggtcta
    28681 gaaaacaaaa tcgtccctga tacaggaatg cctttccata ccttggagat tcaaggattt
    28741 aaacgaaaaa tttccatgca taacatcaag acggtccgat tgtttcttaa gagtatccgt
    28801 caagcaaaaa aaatcctcag tgattttcaa ccagatatcg ttatcggtac tggaggatat
    28861 gtttccggag cggtagtgta cgctgcagcc aaaatgggta ttccaacagt tatccatgaa
    28921 caaaacagtg taccaggtgt taccaataaa tttttaagcc gatatgtgga tcggatcgct
    28981 ctttcattta aagatgcggc ttcctttttt cctgaaaaca aagcagttct tgtaggtaat
    29041 cctcgagcac aagaagtagc agatactaaa aaatcagata ttttgaaaac atttgatttg
    29101 gatccagaaa agaaaacagt cttgattttt ggcggtagtc aaggagcact taaaatcaat
    29161 caagcagtat cagttttttt aagtgaatat attccttctt cttaccaagt tttgtatgct
    29221 tcaggagata gatattatga agagatccta aataaaataa atcctgaaat acaaaatatt
    29281 agtgtccagc cttatatcaa aaatatggca gaggtcatgg cttccagtga tctgctggtg
    29341 gggcgtgctg gtgcaacttc gatcgcagaa ttcacagctc tggggttacc ggcagtgttg
    29401 gttcccagcc catatgttac gaatgatcat cagactaaaa atgcaatgag tcttgttcac
    29461 gcaggagcag caaaaatgat tgcagataat gaactaacag gtgaatcact aagtcagaca
    29521 gtcaacgaaa tcatgggtga tgaagaattg caaaagcaaa tgtgccgagc ttctaaagaa
    29581 caaggaatcc ctgatgcttc aaaacgtctt tatgacttag taaaacaaat aatcaaaaaa
    29641 taaaggcggt ggtcttagtg agtaagaaaa aagaaccaaa aaaattgtct catccgtctt
    29701 ctgatgaaaa caatttgact ccttggcaaa aggctaaccg aaaatatctg gcagaacaag
    29761 agatgaaaaa ccaagagcca aaaaaagaag acataacatt agatacaccc ctttcagatt
    29821 ctgaagaaga aaaaacggtt caagaggaac agcaagaaga aagccagtcc gagaaaaaaa
    29881 tcgaattttt tgaagagatc aagcaaaaga aaaacggtgg accatataac ggttcttttt
    29941 tgaaccggct gcccaattta aaaagtcaac ggcacaaagt actatttcgt cggttagctt
    30001 tgatcatcac tgtattgggt ataccgctga tttttttgat ctattatgtt tctccttata
    30061 gcaaattaca agcagttgca gtatcaggca ataagacagt caacagccaa gaaatcatct
    30121 ctgatacaaa attgtcatta ggagaaaatg tttggggaca gtattttcat cgatctactt
    30181 atatcgatcg ccttaaaaaa gcacagccaa gaatcgaaac agcaaatata catttcaaag
    30241 ggatgaatga attcgatttg gatgtaacag aatataaaga gattgctttg atcgcaaaaa
    30301 acaatcaata ctacccagtg attgaaaatg gaacagtatt agatgaaaaa gtagcaaatc
    30361 ctacaaaaaa tctccctatc ttggaagagt tcaaagactc tgcaaaaatc aaagaattaa
    30421 caaagcaata caatcagtta tcatcagaat tacaaaaagc aatttctgaa atcaagtaca
    30481 cgccaagagc aagcaataaa aatctgatcc aattgaatat gaatgacggc aatcaagtta
    30541 ttgtaaatat caataatctt gcgaaccaaa tgaaatatta ttcgcaagta gccaaagata
    30601 tggatgaaaa aggtgtgatc gacatggaag ttggtatctt ctcgcatcct tacggtagca
    30661 ctcaagaaac ggaagaatcg ggccaatcgg aagaaacaac tgaaagtgaa gaaaatgcgg
    30721 atcaaacaaa cgaatcaagt gaagaggcag aaacgcaaaa agcacaaaca cctggatcgt
    30781 ctagcacaaa cggatcatct gattcaaatg attcaagtga aacaagtcaa atagaagata
    30841 atccaagtac aactccgtca agtttaaatg aaaattgata aaacaacttt aaaaagtaag
    30901 aatgtacttt ctaaaaacca gtaatttatg gtagaattgg aagcagtgaa tatatctatt
    30961 aataagtata ttttaaaacg gacgctagtt gttcgcttgt atatggaata gtaagtagga
    31021 gggaatccca ttcatggcaa aaacaggaat gtatgtaggc cttgatattg gaacgacatc
    31081 tgttaaagtt gtcgtggctg aatatatcga cagccaaatg aatattattg gagtaggaaa
    31141 cgcaaaatca gaagggatta accgaggcat tatcgttgat atcgataaaa cggtccaagc
    31201 gatacaacga gcagtacgac aagcagaaga aaaagcaggt atccagatca aaggcgtaag
    31261 tgtcggtttg ccagcaaaca tgcttgaagt agaaaattgc cagggaatga ttgcagttaa
    31321 cggagattca aaggaaatca ccgatgaaga tgtcagaaat gtggcttcag ctgcccttgt
    31381 tcgttcaata cctcctgaac gtcaaatcgt gtcaatattg ccacaagatt ttactgtcga
    31441 tgggttcgag ggaatcaaag atcctcgtgg aatgatcggc gtaagattag aaatgtacgg
    31501 tctgttgttt acaggaccta aaacgatcat ccataacata cgtaaatgcg tggaaaatgc
    31561 cggattaatc gtcaacgaaa tggtgattac tcctttagcg ctgactgaat caattctttc
    31621 tgacggagaa aaagattttg gtacgatcgt cattgatatg ggtggcggcc aaacaacaac
    31681 ggctgttatg catgataagc aattgaagtt taccaatctt gaccaagaag gtggcgaatt
    31741 tgtcactaaa gatatctcta ttgtattaaa tacgtcattt aacaatgcag aagcgttgaa
    31801 aataaactac ggggatgcat acccagaacg gacatcacca gatgaagaat ttccagtaga
    31861 tgtgattggt cagtcagaac cagtgaaagt cgatgaacgc tacttgtcag aaattatttc
    31921 agctcgaatg gaacagattt tcaacaaagc aaaagatgca ttggaccaaa tcgaagcatt
    31981 agagttgcca ggtggcgtcg tattgacagg cggagctgct agtcttcctg gtgttgtgga
    32041 cttggcacaa gaaatcttcg gcgtaaatgt aaaattgtat gttccaaatc acatggggct
    32101 gagaaacccg gtatttacaa atgtaataag tatcgtggat tattcagcaa acttgagtga
    32161 agtgtatcaa ttggcaaaaa ttgctgttac gggtgaaacc tcagcagctc gtcaaatggt
    32221 agttgaacaa gaaacaacaa acacatatga gagctatgaa gcacctgaag aaacaatcta
    32281 cgatgagccg gaaccaaaag agtccggaga aaatgtaaaa aataaaatca agggtttctt
    32341 tacaaatatt tttgattaat tcatcctggg aggaataaga tatggaattc tcaattgata
    32401 ataatatcaa tgacggcgca gtaatcaaag tcatcggagt cggtggtggc ggtggaaatg
    32461 ctgtaaaccg aatgattgaa gaaaacgtta aaggtgttga attcattaca gccaacacag
    32521 acgtgcaagc attaaaaaat tcaaaagctg aaacagttat ccaattaggc cccaaatata
    32581 ctcgtggttt aggtgctggt tctcagccag aagttggaca aaaagcggca gaagaaagtg
    32641 aacaatcctt acgcgaagct ttagacggtg cagatatgat tttcatcact gcaggaatgg
    32701 gtggaggaac cggtacaggt gctgcaccaa tcgttgcagg tatcgcgaaa gaattgggcg
    32761 cattgactgt tggtgtcgtg acacgtccat tcacttttga aggaccgaaa cgtggccgtt
    32821 ttgctgcaga aggtatcgct cgtttgaaag aaaatgtgga tacattatta attatctcaa
    32881 ataaccgttt attagaagtt gtagataaaa agacaccgat gcttgaagct ttccgtgaag
    32941 cagataatgt tttacgacaa ggtgtacaag gtatctctga tttgatcaca gcgccaggtt
    33001 acgttaacct tgactttgct gacgtgaaaa cagtgatgga aaaccaaggt acagcattga
    33061 tggggatcgg tgtggctagt ggagaagatc gcgtagtaga agcaacgaaa aaagcaatct
    33121 cctctccatt gcttgaaaca tctattgatg gagctgaaca agtacttctt aacatcacag
    33181 gcggtttgga tatgacattg ttcgaagcac aagatgcttc agatatcgta gcaaatgcag
    33241 caactggcga tgtaaatatc attctaggta catctatcaa tgaagaaatg ggcgatgaaa
    33301 tccgtgtgac ggtcatcgca acaggtatcg atgaatcaaa aaaggaacgc aagtctagcc
    33361 gaccagctag acaagcacaa atgcaatcac ctgctcaaaa gactgtatta gatatggatc
    33421 aagctaaacc aacttcttca gaagaagaaa acagttttgg cgattgggat atcagacgtg
    33481 aacaaaatgt tcgtcctaga gttgatgatt ccaattttga caatatcgaa aaaaaagaat
    33541 ttgatacatt caaccgcgaa gaaacaaaat ctcatggcga tgatgaatta aatacgccac
    33601 cgtttttccg tcgtaaaaaa taaaggagag gaagagcaca tgattgctga caacttgcag
    33661 aaaatcaagc aagaaattca ggattcgtgt gctcttgttc atcgtgatcc gaaagaagtt
    33721 acattagtag ctgtaacaaa atctgttgaa tccgctcaag ctgaagaatt ggttagagct
    33781 ggcgttatga attgtgcaga aaaccgagta gataaattat tagaaaagaa acaagattta
    33841 tttgattttc ctgaaataaa gtggcattta attggtaatt tacagcgcag aaaggtaaaa
    33901 ttgattgtaa atgaaattga ttactttcat gcgttggaca gtttaagatt agcagaagaa
    33961 atccagaagc ggttagagaa agaattatcc tgttttattg aagtaaatgt ttctggtgag
    34021 gaatcaaaac atggaatttc accagatgag cttttaccgt ttgtggaatc tttagctgcc
    34081 tgcacaaaaa tcaaggttgt tgggttgatg actatggcgc ctaaagaagc ttctgatgag
    34141 caaatccata aagtgtttgg tacgctgaaa gaattgcgtg atcttgttca agcaaaaggt
    34201 tatgcccatg caccatgtag tgaattaagt atggggatga gtcaagactt tcaaattgcg
    34261 attgaagaag gtgcaacatt tatccgtgta ggaacagcgc tattcaaaga cgaataaagg
    34321 aggaacaaaa atgccgcttt taaacagagg agcaattgct aattttttcg gattagctga
    34381 tgaggaagag tacgaatacc aagattatcc tgaaacaaca aaaaaggctg tacagccaac
    34441 taaaaaaaat acaacacaga atcaatcaac tcattctgta tctgctgcga atactcatga
    34501 aaaacaaaaa acggcgactt cacgtcctgt aacgcatgaa agtattcagc cggctgaaaa
    34561 gaaaaaagta attgaactac atcaatcacc tacaaaaact gtagcgaaag aaagaacagc
    34621 ttcaagaacc tctacacaac ctaaagcaaa tcttgctgga aaaatcactg taatggaacc
    34681 aagagcttat tctgaagcta tggcgattgc taaacgaatc attgctggag aagcaacctt
    34741 agtgaatttt catttagtag aagaaaaaca agcgagaaga atcgttgatt ttctaacagg
    34801 aacagtttat gcactagatg gagatatcaa acgtgttgga gatgagattt ttctttgtac
    34861 accgactggg atggagattg acagttcaac cgctcaatct tttgcagatg cgaatttgtt
    34921 tgatctgtaa gaggaggaac tacgatttat actattgcac tattacttca tttacttcaa
    34981 cgggcagcct atttctatag cattatcttg atggtatatg cattgctctc ttggtttcca
    35041 ggcgggtatc aatcagggct aggacgatta ttagctaaga tctgcgaacc gtatatcagt
    35101 ttgttcgatc gtctgccttt gaggattgga cccttggatt tcacaatttt aggggcaatc
    35161 ctgatcttga acttagccgt acaaggttta gcacagatcg ttatatggat aactgtccat
    35221 ttagtttatt gattgaagca acaaaatctt tattctcaga atagatagga ggggaaaatt
    35281 ttggatgcaa atgtatacca gcactttcgg gcagatgaac gtacatttat cgattcggta
    35341 ggcgattgga ttgaacaggt tcaaggacaa tatgcaccct atctgaccga atttcttgac
    35401 ccaagacaat cctatatttt agaaacactg attcgacaaa gcagtgattt gcgtttcatg
    35461 ttttatggcg gatatgaaca ggcagaaaga aaaagatgtt tgattttccc cgattattac
    35521 gaacctgtag aggaagattt tgaaacagca ttgtatgaaa tccattatcc tagcaaattt
    35581 gccagcctaa gtcatgggaa aatccttgga actttagtgg gaacggggat caaacgctct
    35641 tactttggcg atatcatctc ggacggtgat cgctggcaag tatttattgc aaatgaggtg
    35701 gatcattttg ttgctgcaca agtaacaaag attggaaagg ttgctgtacg cttggaacca
    35761 atcaaataca cagaaatgat tatgccgaaa gatagctgga cacaagaaag aggaacagtt
    35821 agttccatgc gactagacag tgtgatctct gagatttata atatttccag acagcggtcc
    35881 aagcagctga tcgaagctgg taaagtaaaa gtgaactgga cagaaaatac aagacctgat
    35941 tttgtacttg aactattaga tattatttca gtcagaggtt atggcagagt ccagatccaa
    36001 gaacttgagg ggaagacgaa aaaagaaaag tacagggtac tgttcggtgt attacgtaaa
    36061 taaaataaaa aggtggttta ctcatggctt taactccatt agatatccaa aataagacat
    36121 tcccgacaaa aatgcgggga tataatcaag acgaagtaga tgatttctta gatttagttg
    36181 ttcgtgacta tgaggaattg actcaaagaa atcgagaatt agaaaaagca gtcaaacatt
    36241 ctgaagaaaa acttgagtac ttcaatgaat taaaagatgc tttgaaccaa tcaatcatcg
    36301 tagctcaaga tacggctgat aaagtaaaaa caagtgcaag caaggaatcc gaagtgatcg
    36361 ttacttctgc acaaaacaaa gcagatgagt tagttgcgaa cgctgaaaaa cgtgcacatc
    36421 aattaacaac tgatgcagaa gaaaaagcaa gaaaaatctt gacagatgca acagaaaaag
    36481 caagacagct tgcgacagaa actgaagatc ttaagaaaaa aacacgagta ttccatcaac
    36541 gtatcagctt gatgcttgag tctcaattag aacaagtgaa aagtccggaa tgggatgaaa
    36601 ttttacaacc attctccagc tacgtgactg acagccatga agtgatcaaa gaagttttag
    36661 caaaacagct tgacaatgaa aatgatactg atgtaaactc agacacagga gtcatagaag
    36721 cacctatgac ggcatttgat acacaggcta ttgatatctc gatcgtgcct ggtgaaaatg
    36781 aaaacgagta gaacagaaaa gcactttgtt attcttaaag cgagccgatg agagtggaag
    36841 ttcggtaaga cactgcatgg tgaaagatcc tctacttgag acctgctttt ccgaaattag
    36901 agtaagaagc agcggctgac ctcgttattg gtcacagaga gtttgcacta gatagatgca
    36961 actaaatttg ggtggtacca cggcatgttc gtcccttgcg gctgaacatg cttttttgtg
    37021 tccggaaaac aaataaagga gtgtatggtt gaatgaaaat gaaagaaacc ttgcaattag
    37081 gaaaaacaaa attccctatg cgtggtaatt tgccaaaccg agaaggcgag tggcaaaaag
    37141 aatgggaaga aaatcgaatc taccaaaaaa gacaagagtt gaacgaagga aaacctagct
    37201 ttgttcttca tgatggacct ccatatgcca atggtaatat ccatttagga cattcattga
    37261 ataaaatcag taaagacatt atcattcgtt caaaatcaat gtctggctat cgtgcaccat
    37321 atgttccagg atgggataca catggattgc cgattgaaca agtattggct aataaaggcg
    37381 tgaaacgtaa agaaatgtcc atggcagaat acttggaaaa atgtcgtgaa tatgcacttt
    37441 cccaagtaga taagcaacga gcagatttca aacgcctagg agttgcagga gattgggaac
    37501 atccttatgt tacattagat ccttcttatg aagctgcaca agtacgcgta tttggtaaaa
    37561 tggctgaaaa aagctatatt tacaaaggat tgaaaccaat ttactggtct ccgtcaagtg
    37621 agtcttcttt agcggaagct gaaattgaat ataaagatgt aaaatctcca tctatttttg
    37681 tagctttcaa tgtcaaagac ggcaaagata ttcttgatga agatacagct tttgtcattt
    37741 ggacgacaac accatggaca ttgccagcaa accaaggtat tgctgtaaat ccaaaattta
    37801 cgtatgtatt ggtggaagcc gatggccgta agtttgtcgt agcgaaagat ttgctagaaa
    37861 cagtccaatc agaaatcggt tgggaaaatg taacagttct aaaagaattt gctggacaag
    37921 aaatggaata tatgactgca agtcatccat tctatgaacg tgaatcactt gtgattttag
    37981 gcgatcatgt tactttagac gctggtactg gattagtcca tactgctcca ggacatgggg
    38041 aagatgacta tatcgctggt agcaaatata aactatctgt agtgtcacct gtagactcaa
    38101 aaggattgtt tacagaagaa gcacctggat ttgaaggtat cttttatgac aaagcaaatc
    38161 ctatgatcac tgatctatta aaagaaaaag gagctttgct gaagctagat ttctttactc
    38221 atagttatcc tcacgattgg cggacgaaaa agcctgtgat ctatcgggca actccacaat
    38281 ggtttgcatc tatcgataaa ttccgtcaga atattttgga tgaagtagaa aaagtagagt
    38341 gggttatccc atggggtaaa acacgtttgt ataatatgat ccgtgaccgc ggcgattggg
    38401 taatttcccg tcagcgtgca tggggagtac cacttccgat tttctatgct gaaaatggag
    38461 aagcaatcat cacaccagaa acaatcgaac atgtggcaaa attatttgct gaacatggat
    38521 caattatctg gtttgaaaaa gaagcgaaag aacttcttcc tgaaggattt acccacccag
    38581 gttctcctaa tggcgaattt acaaaagaaa aagatatcat ggatgtctgg tttgattctg
    38641 gttcttctca tgaagctgtt ttacgtcagc gaccagaatt atcattccca gcagatatgt
    38701 atttagaagg atcagaccaa tatcgtggct ggttcaactc aagtatcacg actagtgtgg
    38761 ctatcaatgg tgtagcacca tacaaatcag ttatttctca aggatttgct ttagatggtg
    38821 aaggccgtaa gatgagtaaa tctttaggaa acatcattgc accagaaaaa gtgatcaaac
    38881 aatttggtgc agatatcctt cgcttatggg tatctagtgt agattatgaa gcagatgttc
    38941 gtgtttctat ggatatctta agtcaagtgg cagaagtata tcgtaaaatc agaaatacga
    39001 tgcgtttctt attagcgaat acagaagatt tcgatcctaa aaaagatact gtttcgtaca
    39061 atgatttacg ttctgtagac aaatatatga ccgttcgatt gaaccaagta atcaaagaaa
    39121 tccgagaaga agggtacgac aaatataact tcatgcatat ttaccgtaca gttatgaact
    39181 tcttgacggt tgatctatcg tcattctacc tggattttgc taaagatgta gtttatattg
    39241 aagcagaaga tgattatcaa cgtcgttgca tgcaaactgt tttctatcaa acagctgtag
    39301 ctttgacaaa attattgact cctatcattc cgcatacagc agaagaaatc tggtcattcc
    39361 tgaaagaaga agaggagtac gttcagttaa gcgagtttcc tggttatgaa gagtttgcaa
    39421 accaagaaga attgatggat acctggactg ctttcatgga cttccgtgat aatgtcttaa
    39481 aagcattgga agaagcaaga aacagcaaat tgattggtaa atctttggaa gcaaaactaa
    39541 ctgtttatcc aaaacaacaa gttcgtgaaa tgttaaaagc tttggatgca gatattgcac
    39601 aattattgat cgtttcacca gactactttg aagttatggc agcagatgaa caagtgccag
    39661 ataacgctat ggtatttgat gatgttgcta ttacagtgga aaaagcagac ggggaaactt
    39721 gcgatcgctg tcgccaagtt cgtaaagatg taggagtaga tgaaaaactt cctcatctat
    39781 gcggtcgttg tgcaaaaatc gttgaagact tctatcctga agctgtagct gaaggattcg
    39841 aagaaaagta aaagggaaaa gggggtgtga catgttcttt gtcacacccc cttttgttat
    39901 caatcgttta attttccacg ttcacgatac cagttatctg gttgccagat ccattcgaat
    39961 ccgtcttttt caagtagttc gaaagctgct ttaggtccca ttgatccagc tttgtagttg
    40021 ggaaaatccg gttgtgtttg gtcccaagct tttcgaatga catccacgat atgccaagat
    40081 tgagcgactt cttcccaatg agagaagttc gttccatcac cattcaacgc atctaatagt
    40141 aatttttcgt aagcttctgg gctattttca accatttctg cactatttct aaattccaat
    40201 ttgattggtt ccgtttcaaa tccttgccct acttctttcc cattcaagga taaagaaaat
    40261 ccttcagttg gctgaatata gatcgttaga acattaggtg gaagtgtcgt atcgtcacat
    40321 ggttcatcga cagatgtttt gaatacatta acaggaactt gtttgaatac gatgttgatc
    40381 cgagtccctt tttcagtcaa acgtttgcct gtacgtacat agaaaggaac acctgaccaa
    40441 cggaagttat cgatagtgaa tttgcctgcg acgaatgttt cagtctctga ttgagcatcc
    40501 actttgtctt cttcgcgata gcccttgaat tgtacaccat ccaattcacc ttgagcatat
    40561 tgcccgcgaa cgaagttttc taacacttct tcttcgttat aaatgcggat agctttcaat
    40621 gctttgatct tttctgtacg gatttctttt tcggaaaatg ctactggcgg ttccattgca
    40681 agtaatgcta caacctgtaa aatatgattt tgcaccatgt ctttaagtgc gccactatga
    40741 tcgtagtagc ccccgcgatc ttctacaccg aggttttcgc caaaagtaat ttggatattg
    40801 tcgatatagc gattgttcca ttgagcctca aaaatattat tggcaaaacg gatagctgag
    40861 atgttttgga tcatttcttt tcctaaataa tgatcaatcc tgaaaatatc ttgttctggg
    40921 aatacttctc tgatttgctg gttcaattca aaagcagact ggtaatcaga accgaacggt
    40981 ttttctatga tcaaacgatt gaagccattt tttgtcataa tattttgtga tttcaaatga
    41041 gaaacgatcg ttccaaaaaa ttgaggtgcc atagccaaat aatagacacg gttaccgttt
    41101 aattgatagt gtccatctaa tcggtcagac aattctttta atgtctgata atgcgttgaa
    41161 tcatttacat catgggattg atagtaaaaa tggcttgcaa aggtagttgc ttcttcatct
    41221 gaaggattca atgcacggat tgtttctttg acaacttcac ggtagtgctc atcgctccag
    41281 ggacggcgag cggttccgat tacagcaaaa tgctcgccta aatttccttt tctgtaaagg
    41341 cggaacaaag acgggtacaa tttacgctgg gctaggtcac ctgtaccgcc gaatatcgta
    41401 aacaatacat ttttttcatt agacatgtgg atacactcct ttatatttta tcgacaaaaa
    41461 tcgtgctggc cgccttaaag ctgacgggga ttttctttct atcattttgg atagtgatcg
    41521 gtccttcata agctgcgatg tcaacgatct catattcttc gttgatattc aggtcaatag
    41581 tgactaagta atccaacaat tctttttcat caagcactcg tgcgatacgc acttttgtac
    41641 cgacaggata ggactccaat gtttgtcggc gttcttcatg gacaacttcc tcttttttag
    41701 gaatcgatcc tccatgagga caatgttcag gatgattcaa atagttttct aaatgatctg
    41761 caagatgttc cgaagtcaca tgttccaata cttcagcatc atcatggact tcattccagc
    41821 tatagtctaa atgttcaact aaaaaaactt cccataagcg atgtttgcga atcagactgc
    41881 tagctttttg cagtccgctt gcagtcaatt gtacaccttg ataaggggaa tgttctacaa
    41941 gctcttcttt cacaagttta gaaatcattt cactgacaga tgcagctgag acatctagac
    42001 tagatacgat ttgtttattg ttgactttcg ttgtatctcc acctagttct aaaatgattt
    42061 tcaaataatc ttctcgattt ggtgtcatat tcgatcatcc tttcatggta aaaaaccatc
    42121 attagtaagt gtaacaaaaa aaacgttaat tgactatttt tctaaataaa aaacaaagga
    42181 tttttagacg tttttacaac aaaaaacgaa agatgataga gaagactcat tctttcgttt
    42241 ataaaggata tatatataca ttgggaggga tctatttttc agataacaac ttatatgaag
    42301 tgtctgcaga ttcgtcataa agagtttgtt cgggataaga aaagataaga taaaaaccat
    42361 actgatcacg tacgactaaa cgatattcat ctatttctac cacagatgtg ccaagagact
    42421 gaccgttgat caaaaacgtg taatcagatg taatctgtaa ttgttgtttt tgctggctat
    42481 cactaaagaa ccatgtacct tgatactttg agagtccttc tgacttttcc gcttgttttt
    42541 tctttttcaa gtaaataaaa cttgagagaa cggtaatgag tgaaccaaga aaatagatca
    42601 ttttttttat caccaaatca ctcctttatg agaaaataat cgcatagaca tattctttct
    42661 tctttatttc attgtaccga ataaaagcta aaatttctct aagactttcg actggttttt
    42721 ttactgtttt tctacaaatt atgataggat acatagtagg aagaaaggtg acataatttg
    42781 aagtatcagc aagtggaaat agcttttttt attgaccgcc cgaatcggtt tatagcacat
    42841 tgtatgaaaa aagacggaga aataattatt gcgcatgtca aaaatacagg tagaggaaaa
    42901 gaggtcctga taccaggcgc tgaagtggct attgtatttg ctcctggacc taaacggaaa
    42961 accgcctatg atctgattgc tgtaaaaaag aagggtgact ggtttaatat cgatagtcaa
    43021 ttgcctaatc ggctggcaat tgatggtatt ttagacggca cgatcctttt accaggatta
    43081 actggtccat ttacggtata caagcgtgaa gtaacttatc ttcactccaa atttgatatt
    43141 tatttagaga cggtggtcgg acaaaaagca tttgttgaag tcaaaggtat gacacttgaa
    43201 aataaagcga ttggtgcttt tcctgatgcg cccacactca gaggattgaa acatatagga
    43261 gaattaacgt atgccgcaca agatggttat gccgcttatg tcttgtttat tgctcagttc
    43321 gaacatcttc atctggcaac gatccatgaa gaaatgcaac cagcactagc tgatatggtt
    43381 cgacatgcgc agcaaagcgg agttcaaatc ctcgcctata attgtcaagt atcggcagat
    43441 gaggtaactg tcaagcaggc cattccattt gatgtagata tcccttttga agatcctata
    43501 caatagaaag aaggtaaaat aatgatcaac ttaggtatta tcggaacaaa ttggattact
    43561 catcaatttg tccaagcagc tttaagcaca aaaaaatatg aattgacagc ggtatattct
    43621 agacatttgg aaaaagcaca ggaatttggt tctcattatg aaggggatat cgaattcgca
    43681 acagatttga agactttttt caatattact catatggaca ccatttatat tgcttctccg
    43741 aatagtcttc actttagtca agctaagcaa gcaattttgg caggaaaaaa tgtgatagtt
    43801 gaaaagccag ctttttcgac gccaaaagaa atggatgaaa tcatccattt ggcaaatgaa
    43861 cacaatgtgt atttttttga agcagcaaga aatatccatg aatcaggttt taaaaagatt
    43921 gccgattact taccattaaa agacgagata atcggtgcaa atttcagcta tatgaaatat
    43981 tcttcgcgct atgaccaagt attagatgga gaagaaccga atatcttctc gactcatttc
    44041 tcaggtggtg cattgatgga tttaggtgtt taccttgtat atgcagctgt tgcttggttt
    44101 ggaatgccaa aagaagtcca ttatttccca agaaagattt ctactggtgt agatggtctt
    44161 ggatggggga tcttacgtta tgagaatttt gatgtatcga tccagccagg taaaatcggt
    44221 gattcttatt taccgtcaga gatttatttt gactcaggaa cgctagtgat gaatggagtc
    44281 aatgcaattg aacaagcaca atttcatgat cgcgcgcata aagaaatcat tgatttggcg
    44341 gtaacagctg cagaaaatcc aatggcagat gaagcagagg attttgccaa tgtcatggct
    44401 catccgaaag atccagcttg gggattgttg tatgaagagt ggatcgagct agctagaaat
    44461 gtgaaccaag tgatttatga tttaagaaaa aatggcggca tcaaattcga tgcagataat
    44521 gaggaagatt tttaatgaac gaatttgtac aagtatttcc cgaaaattgg cagaaaaaat
    44581 atgaggataa agggtttatc tcaccttctc ttattcaacg agcagttttt cagccactaa
    44641 gtaataaaca aagtatagta gcggtttctc ctacaggttc aggtaaaaca ctggcttatc
    44701 tttggcctct actactaaac gtggaaccag gtgaagcaag tgcgttggtc atttttgctt
    44761 cttctcaaga actagctatt caagtagcag atgttgcacg tgaatgggga aaagacaaag
    44821 agttgaaagt ccaatctttg gtgggcggag ccaacgtcaa acgtcagatt gagggtttga
    44881 aggaaaaacc agaaatcttg attggtacac caggaagaat attggaattg atgaaagcaa
    44941 aaaaaataaa agcacatcaa gtgaaaacaa tggtatttga tgaagcagac cagctatttg
    45001 acgctgggaa cagccaaatc attgatcaaa ttcttcatca agctccaaca gagtatcagc
    45061 tagctttttt ttcagcgaca gcagatcgtt cccttgaaag cattgaaaaa atcactggga
    45121 aaacgtttga gacaattgac gtgacagcag aggacgattc tcgaaaagga ctgagacatt
    45181 actttttacg tgtaccgata cgaaagaaag atgactactt gagacgtctc acgcatatag
    45241 aagattttca tggtctgatt ttctttaatc aactgactga attaggcgta atggaagaaa
    45301 aattgctgta tagaggtgta ccggtaggaa gtctggcctc tgatcagaac aagctactcc
    45361 gaaaagcagc gctagaacaa ttcaaaaaga aaatggtcag agcacttttg accacagatg
    45421 tggcggctag aggtttagat attacaggtc ttccttatgt agtgaatgca gaagttccgt
    45481 taagtgaaga agcttatttg catcgttcgg gaagaactgg tcgaatgggt aatgaaggaa
    45541 tagtaattac actcgtacaa gataataatc taagagattt aaaacggctt ttacgtccat
    45601 caaatataac acttgaagag atttttttat acggaggtaa gctgcaaact gagcagccaa
    45661 ccaaagaaga tgcagctcct gcagtaaata aaaagtatgc gaaaactttt tctggaaagg
    45721 agcagaaccc agaatataac aaagaaaaaa taagaaaaaa agttaaaaat aaatcaaaaa
    45781 aagagagaaa taaaggagca aggcgcaaat aagcgccttt ttatgctatg attatgaaaa
    45841 cgggaacaaa attggttgcc agaggagggg ttatgtgaaa gaacgtcaag aagagatctt
    45901 gcagctattg acagaaaacc aacaaggatt gacagcaagt gatgtagctg agcgcttaac
    45961 tatcgatcga agcaatgcaa gccgatattt aagtgaacta tacaaggccc atcatattgt
    46021 aaaaaccgct ggccgaccag tcgtttactc tttgccaacg gaaaagtcaa aatctgatga
    46081 agtgcatgtc gactcttcta cgcaagtgac ttttgaaaca ctcgttggag aaaatgattc
    46141 tttaaaagtc agcatacagc aggcaaaagc tgcgattctt tatccaccgc gggggttgca
    46201 tacgattatt tttggagaaa ctggtacagg gaagtcgatg tttgctgaat gtatgtatca
    46261 ttttgcaatc gattcagaga tgctttctgc agatgcgcca tttgtttctt tcaattgtgc
    46321 ggattatgct caaaatccgc aactgctctt tggacacatc tttgggatca aaaaaggggc
    46381 ttacacagga gcagctcaag atagtcccgg tctaatagcc aaagcagacg gcggtatatt
    46441 gtttttagat gagatccacc gtttaccgcc ggaagggcag gaaatgctgt ttactttcat
    46501 tgataaaggt atttaccgtc cgcttggaga aagtgcacaa gtacatgaag catctgttca
    46561 gatcattggg gcgacgactg aatcttcaga aagtttctta tctacgttca atcgacggat
    46621 tcctatggca atcaccttgc cgaatcttgc ttcacgttca ctggatgagc gttatgaaat
    46681 catctctctt tttatcaaac aagaagcgaa tcgtttgaat caacgtatcg atatcgaaaa
    46741 agaagcgatt cttgccttca tgctttatga tgcggaagga aatatcggtc aagtgaaacg
    46801 agacttgaaa ctagtctgtg caaaatcttt tttacattac cgtactcatc atgagaaaaa
    46861 gctaatcatt cgaaaagaag aactttccct tcaagtacaa aaaggattac tgaaaatcaa
    46921 ggaaataccg gaacgtctcg accgattcat ggacagcaaa agtcagtatt tgacatttga
    46981 accaggattt gctgatgttg tctggtcaca agatccagaa cgaaacatgc aggtctataa
    47041 cgatattgag gaaaaagtca cttctctttc cgaaaccggt gtggagaaca ttgatctaga
    47101 gaccctcatt tcaaaagatg tggatgctta tttccagaca tatgtcaaag aattgactaa
    47161 aagttcgatt cccaaagatc ttttgccaga tgaaatctgg cggttaacca atcaattgta
    47221 tgatgtggct gaaaaacggt tagaccgtat ttataatgaa aaagctcgtt ttgcttttgc
    47281 tttgcatctg caaagtacat tggatcgtgt gagagaaggt catatgatcg tccacccaga
    47341 tttgaataac gtacggaaaa attttaagag ggaattccag gtagcaatcg atctttcaac
    47401 gatcattgaa gaagaagaac aagttgagat tccttttgat gaaatcggct ttattagtat
    47461 gtttctttct atcaatctag gagaaagtga gcctgttaaa gaaaacaaag tagatgttct
    47521 catattgatg cacggcagac agactgcttc aagcatgttg gagatggcac aagaactatt
    47581 aggaacaaaa gtagggaaag cttttaatat gccactggtt atggaagttc aaacaatgta
    47641 tgaagaagtg ttacaatatg tcaaaaacta tcaagagcat ttgacgaatg gcctactctt
    47701 gttaacagat atgggatcat taaacacctt cagtgatttg atttatgaag agactggcat
    47761 tcggacgaaa gctatcacga tgacaagtac catgattgtg atagaagcga tacggatggc
    47821 aagtgttgga agaagcttag aagatattta tcaaaatatc cagctttcct ttgaatcgat
    47881 cgtacgtgat cagttcaaag cggaaaagtc tgaaacttta cagcgtaaaa aagccgtaat
    47941 cgtgacatgc tttactggtg aaggagtagc tgccaaatta tatcagcgta ttatgccagt
    48001 gattaatcaa aataaggtag aactgatcca gatgcagttt ttagaacgtg aatcattcaa
    48061 aaaacacatc gatgccttat tggaagaata tgagatcaaa gcaattgcgg gaacagttga
    48121 tgttgattat caaaatatcc catttttttc agcctatgac gtttttgatg atgaaaaact
    48181 aaatatttta aagcgtatag ctgatgatga gatcccgaca gctaaaattg tcgattcact
    48241 tgctggatca ttaagcgatg tcttttctat tgatgaactg atccgttcat tgcaaaagac
    48301 agttcagcag atccagaccg atttgtcgat tatcgtggaa cctggagtag atactggaat
    48361 cgttatccat ttagccttca tgttagacgg attgatcaaa caagaaagcc atcggcaatt
    48421 tgagaaactg tctattttct caaaaattta tcgtttggaa atggatatcg ttcgggcaaa
    48481 cttgatggca ctagaacgaa aatatcaggt aaaaattcca gaagatgata tcgcatttct
    48541 tacacaaatg tttcttgaaa ataaaataaa aaaatgaaca cactaatcat actgtttaca
    48601 cactaaacat ctgtgtgcgc actataaaaa agagctaaaa agcatgaaaa cgctatttca
    48661 gctctttttt ttattggcat gcttcttgcg ttatataaaa gtgtaaaaaa ataaaaggag
    48721 tgttaaaaat ggcaaaaaaa acaatcatgt tagtttgttc agcagggatg agtacgagtt
    48781 tattggtcac aaaaatgcaa aaagcagcag aagaaaaagg gatggaagcc gatatttttg
    48841 cggtctctgc ctcagatgca gataataatt tagaatctaa gaatgtcgat gtattattat
    48901 taggtcctca agtacgtttc atgaaagctc aatttgaaca aaaacttgcg cctaaaggta
    48961 ttccattaga tgtcatcaat atgcaagact atggcatgat gaacggagaa aaagttcttg
    49021 cacaagcaga aaatctaatg aaataaatca agtatgcagt tgggggtata acaacgtgga
    49081 agatcaacaa aatctagaag ccatcatggg gttaatcatg tttggcggta atgcaaagag
    49141 cgatgcaatg gaagcgattg cggctgctaa aatcggtgat tttgagttag cggataggaa
    49201 aatcgctgat gcagaagaat cacttataca agcgcatcat gcacaaacag acatgttgac
    49261 acaagaagca caaggaaatc atatgcaagt gacattattg actgttcaca gtcaagatca
    49321 cttaatgaca tctattgcgt ttactgattt agcaaaagaa atcatcgaac tttatcgtcg
    49381 cgtatacagc gaataaatac aacttctttt ttgaacggaa gaagaaaaat ttttgaacgg
    49441 attttttatt ttgttataag gagaactctc tttatataaa aaacttatag gaggaagtaa
    49501 aaaatgaacg gactatcaag ttggatggag aaatacatcc tgccggttgc cgcaaaaatc
    49561 ggagcgcaaa aacatttagt tgctttgcgt gatgctttta tcggcacact gcccgcaaca
    49621 atggcgggat cagtcgctgt aatgatcaat gcaattattc gtgatttacc gcaacagttt
    49681 aacagtacct atgctgacag tttagcagca gacaaaggaa tctttgccgt attgaatgtc
    49741 atcatcggaa tcaatggttt tgtctggaac ggtacactag caatcgccgg attgatcttt
    49801 gccttttctt gggggtataa tcttgcccga gcttatggag taaatgatct agctgggggg
    49861 atcgttggtt tagctacctt gattcaaggt atttcctttg gtccagcatt atcagcagca
    49921 ttagatgtga atgttccttc aaatgtcgca gatgcaatca atagttctga gattggtgca
    49981 acgattgcag atggcacact gtcagttgga ttatggggtt ggctaaaact agatcatttg
    50041 aatggaaacg cttattttac agttatgatc atgggagcta tttcagtcat tattttttgt
    50101 aaattgatgc ttgccaacat tacgatcaaa atgcctgatt cagtaccacc agcagtatct
    50161 aaagcatttg cagccatttt accagcgacg atcgctttat acgtcattgc tattatcaat
    50221 tatatcgtag gtcgtgtagc tgatgggcaa ctgatcattg atttggtgca aaaatatatt
    50281 gcagaaccat tcctaggatt atctcaaggg ttgggagcag tattgatcgt cacagtattt
    50341 gttcaagtat tctggttctt tggtattcat ggaccaaatg tattggcacc agtgctagag
    50401 ggaatctggg gacaagcaca actaatcaat attgatattt tccaaaaagg ttataacgga
    50461 aaaactggta caccagccgt gttagatgca atcgaagatg gaaaagctta tatgtgggtc
    50521 cgcggatcgt tcgatgcttt tgcatggttt ggcggatcag gcggaacgat cgtcttactg
    50581 atagctatct tgattttctc taaacgtgca gattatctaa cagtagctaa attatccatt
    50641 gggcctggta tcttcaatat caacgaacca gtgatgtttg gtctgccaat cgtattgaat
    50701 gcaatcatgt ttatcccatt cttgattgct ccagtagtag caacagcaat tggatggaca
    50761 gctacttatc ttggcctagt cgctccggta tcgcagcaag tcacatgggt agtaccgccg
    50821 ttcttattgt cattcttagc aacaggagcg gattggcggg caccgatcgt aacattagta
    50881 tgtatggtcg tgactttctt gatttgggca ccatttgtca tctctgcaaa caaaatggac
    50941 cctgccgcag gcgaacaaca ataagatgaa cgtgaggagc gggacgcttt aagtttcgtt
    51001 cctcataatg aattttcgag gaggaactga aatgattaca attgccagtg caatgcaaca
    51061 agatgaaatc aaagaattgt tagaaggata caatgaagaa ggaatgactt ttacatttaa
    51121 agaaaaacaa ggaatcaagc tgttctttga aacaagtgca gaagatccgg aagttgcggc
    51181 taaaaaagca aaggaactaa tcaaagcaca gccttgggga acagtccttt attttcaatc
    51241 aacagctgtt tagaaaatgg tttaaaaaga taggatggtt gagatgataa aaaacggttt
    51301 atttttatgt agtattggtt ctttgatcgt gttgtatagt ttgaatccac attcgcaaag
    51361 gtatgatttg tatagtatgg tcacaggtct ttttctaaca ggattaggaa tctatttctt
    51421 catgaagggg aaaaaaagag aagaagcaaa ggagaaaaat aactaatgga aatcacgaaa
    51481 aaattaaata ttccagcatc gtttttttac gataaagtta tggactctgt ccttttcgat
    51541 gtacgtaaag cgaccggcaa atcagtaact agaaaacagc tgaaaaattt ggagtacgtc
    51601 aaacaattct cgcaaaataa ccgggctcgc ataaaaatcg aagaagtcat tgaaaaccag
    51661 tcttataagt ttcgtacatc gacaacgaag aatgaattcg tcgttcatta tcaaatcaaa
    51721 ccattggacg attcgacctg tgaggttcat tacacagaaa aaatggagtc ttatggcatg
    51781 cttcaaaagt tgaatgatat gcttttagga acaatcttga tgtattttaa acggagacgt
    51841 ttcaaaaaaa tgctagatat gatcgaagca acgtattaaa tcaatgaaac atggtactat
    51901 ttagttattg aaaaatgaag taagctagaa ggaatttttc ataggaagat agatctttcc
    51961 tgcgaatcct cactagcttt tttgtaggaa ggggcagcaa aatgacaaac aaagtaagag
    52021 agctaggtgt atcttcgatt gaggaaatgt ttgatttagc cacttatgca tttaatgggg
    52081 cagatacacc tgaaaggcga gaacgttttc gtacacttgc ggagaattct tggaattacg
    52141 gattttttga tgaggaggat cggctaacaa gtcaagtcat ggcgacccct tttccagtga
    52201 atttttacgg acaagagtac ctaatggccg gcattggcta tgttgcttct tatccagaag
    52261 caaggggaca aggcgggatc aatcagatca tggaaaagat ccttgaagat tgccgtgatc
    52321 gtaaagtaag cttatcttat ctggcgccat tctcctatcc cttctatcgg cgctatggct
    52381 atgaacaatc atttgataaa atcagttatc aactaaacag tcgtgatgtg ccgtttatca
    52441 aaaaaaaatc tggaaaaata aagcgtatgg attttgaaga tgcaaaagct gcaatcagaa
    52501 gaatttatga acagatgccg gaaaaccaac gagctggtct gaagcgcgaa gactggtggt
    52561 atacgtataa attcaagcaa aaaggcaatt accagtttgc tttgcatttt gatgataatg
    52621 aggaagcaac gggatatatc gtttatcagc ttgcaacgtc ttcctttatt attgcggagt
    52681 ggggattttt ggatcatgat gctttttgca gccttgttcg cttcgtaagt tctcacaatg
    52741 gtgcttttga aacatttgaa tattcatgcg gctacggcgg taatgatcaa aattatctat
    52801 tagaaaatcc atttgcttct gtaatgatca caccttatat gatggcgagg atcgtagata
    52861 tttctttgtt tttggaaaat tatccattta aaaagagaca agctgcattt gccctacaaa
    52921 tagaagaaga tcattatgcg aaatggaatg aaggaatcgt ggaagtaaca atcgaaaatg
    52981 gaaaaaatca tctctcaaaa gtagaacaaa caaaactccc aatcgttcga ggaagtatcc
    53041 aacatatcac gcagctatta ttagggtatc gtaaagtgga agagttggta ttccaagaaa
    53101 gaatacaggt gacaaaagaa tatgaagaaa cgttaagctc aatcttgcca cagcaacagc
    53161 caattcttaa tgactatttt tgataaaaaa cgggtgatga caatattttt gtcaccaccc
    53221 ttttgttttt catttggcat ttgaaggaaa atgttaaagt aaatcctctt ttttcgtaaa
    53281 tttgaatttt ttgttttctt ttgttttagg gttttctttt ttgtggtctt ggattgcttc
    53341 tttcgtaatc ttttcccgat tttttttaga tgaatctaag cgttctgcgt atactttttc
    53401 ttttggctta tcattcattg gaaaggacct ccctacatta tttcattcta cctctagttt
    53461 atcatctgtt aaggactagt ggaaacgttt tgcctaactt ataagctgat ttttttagca
    53521 gataagcttg caagtaaaaa acaaacaagc tacaattgat ttattgaaat tgttcctgta
    53581 gctcagtagg atagagcaat cgcctcctaa gcgataggtc gctggttcaa ttccagtcag
    53641 gaacgtataa ataacccctg tatcctttgc ggtacagggg tttgttttgt atttgtgcca
    53701 aaaatgtgcc aaactagtta tcatttttat ataaactatc gaacacagta ttgatttttt
    53761 ctttatcctg atcttcaagc tctcgaacaa tatgaagat
//
LOCUS       DAZKCY010000005        51362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000005 DAZKCY010000000
VERSION     DAZKCY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.005"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2412
                     /locus_tag="ACG8XC_000269"
     CDS             <1..2412
                     /locus_tag="ACG8XC_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189999.1"
                     /translation="FIFSPSAEAVGATPPAISYDPYTYENIVPPNGGRSAVNGGKLVQ
                     LTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESHVKLTNVLDLVNITAQGFAFVAII
                     NLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGSLLSAGSHTLRFGMGSWDTNTLLA
                     DIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDKYDTSPNKILTKGKLQPSMSKKAV
                     FSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGGNLNPGAYAFSASAEINTWNSYIS
                     TSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLTMGLTEGRFDQLPFSRFDRVVNYF
                     TGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQGGLLSPVALKVLEQYKLDGTVLP
                     TNLAADSQWGKLLSSASYQVTANWSATELKKGSIHYRLYSKGTQKLATGWTDQLFQTI
                     QSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTNMYPSFAYKWQSEQAGITTLPQIT
                     VANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQENVTFTLTKSGDAITDKKITISL
                     PENTTYINGSLKLNGTTIADTGIQQGVSVPADLLSKIGDTIHLTYNYQLNTVDTSVQS
                     VSILTKAAVLSSNITLADGAKLPNPVVQTSAKTILVPKQELTLVNVPDDFTFGNDLHK
                     PLKTSYYEAKVDFSFDVRDTRLPSTSPWQLTGTLTSLFKNDQGQELSGTKLYFNHSGS
                     KQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLLEVNSSTNAQPGATYQGMVTWELT
                     AGPTS"
     gene            2468..4027
                     /locus_tag="ACG8XC_000270"
     CDS             2468..4027
                     /locus_tag="ACG8XC_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9190000.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            4024..5388
                     /locus_tag="ACG8XC_000271"
     CDS             4024..5388
                     /locus_tag="ACG8XC_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9190001.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            5559..6629
                     /locus_tag="ACG8XC_000272"
     CDS             5559..6629
                     /locus_tag="ACG8XC_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9190002.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            6644..7312
                     /locus_tag="ACG8XC_000273"
     CDS             6644..7312
                     /locus_tag="ACG8XC_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190003.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            7332..7910
                     /locus_tag="ACG8XC_000274"
     CDS             7332..7910
                     /locus_tag="ACG8XC_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9190004.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            8313..9224
                     /locus_tag="ACG8XC_000275"
     CDS             8313..9224
                     /locus_tag="ACG8XC_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9190005.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(9480..10397)
                     /gene="rnhC"
                     /locus_tag="ACG8XC_000276"
     CDS             complement(9480..10397)
                     /gene="rnhC"
                     /locus_tag="ACG8XC_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFP9190006.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            10599..11036
                     /gene="zapA"
                     /locus_tag="ACG8XC_000277"
     CDS             10599..11036
                     /gene="zapA"
                     /locus_tag="ACG8XC_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9190007.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            11094..11642
                     /locus_tag="ACG8XC_000278"
     CDS             11094..11642
                     /locus_tag="ACG8XC_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFP9190008.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            11757..14117
                     /locus_tag="ACG8XC_000279"
     CDS             11757..14117
                     /locus_tag="ACG8XC_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFP9190009.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            14204..14518
                     /gene="trxA"
                     /locus_tag="ACG8XC_000280"
     CDS             14204..14518
                     /gene="trxA"
                     /locus_tag="ACG8XC_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFP9190010.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            14700..16478
                     /gene="uvrC"
                     /locus_tag="ACG8XC_000281"
     CDS             14700..16478
                     /gene="uvrC"
                     /locus_tag="ACG8XC_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP9190011.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            16623..17522
                     /locus_tag="ACG8XC_000282"
     CDS             16623..17522
                     /locus_tag="ACG8XC_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190012.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            17515..18777
                     /locus_tag="ACG8XC_000283"
     CDS             17515..18777
                     /locus_tag="ACG8XC_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9190013.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            18938..20836
                     /locus_tag="ACG8XC_000284"
     CDS             18938..20836
                     /locus_tag="ACG8XC_000284"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFP9190014.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(21135..21854)
                     /locus_tag="ACG8XC_000285"
     CDS             complement(21135..21854)
                     /locus_tag="ACG8XC_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFP9190015.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    22044..22091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            22191..22523
                     /locus_tag="ACG8XC_000286"
     CDS             22191..22523
                     /locus_tag="ACG8XC_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190016.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            22510..24513
                     /locus_tag="ACG8XC_000287"
     CDS             22510..24513
                     /locus_tag="ACG8XC_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFP9190017.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            24532..25002
                     /locus_tag="ACG8XC_000288"
     CDS             24532..25002
                     /locus_tag="ACG8XC_000288"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFP9190018.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            25023..25607
                     /locus_tag="ACG8XC_000289"
     CDS             25023..25607
                     /locus_tag="ACG8XC_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFP9190019.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            25622..26608
                     /locus_tag="ACG8XC_000290"
     CDS             25622..26608
                     /locus_tag="ACG8XC_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFP9190020.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            26598..26909
                     /locus_tag="ACG8XC_000291"
     CDS             26598..26909
                     /locus_tag="ACG8XC_000291"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFP9190021.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            26931..28712
                     /locus_tag="ACG8XC_000292"
     CDS             26931..28712
                     /locus_tag="ACG8XC_000292"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFP9190022.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            28705..30081
                     /locus_tag="ACG8XC_000293"
     CDS             28705..30081
                     /locus_tag="ACG8XC_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFP9190023.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            30088..30723
                     /locus_tag="ACG8XC_000294"
     CDS             30088..30723
                     /locus_tag="ACG8XC_000294"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFP9190024.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            30753..32108
                     /locus_tag="ACG8XC_000295"
     CDS             30753..32108
                     /locus_tag="ACG8XC_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP9190025.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(32300..33694)
                     /locus_tag="ACG8XC_000296"
     CDS             complement(32300..33694)
                     /locus_tag="ACG8XC_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9190026.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            33786..34757
                     /locus_tag="ACG8XC_000297"
     CDS             33786..34757
                     /locus_tag="ACG8XC_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFP9190027.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            34754..35535
                     /locus_tag="ACG8XC_000298"
                     /pseudo
     CDS             34754..35535
                     /locus_tag="ACG8XC_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(35704..36075)
                     /locus_tag="ACG8XC_000299"
     CDS             complement(35704..36075)
                     /locus_tag="ACG8XC_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9190028.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            36209..36652
                     /locus_tag="ACG8XC_000300"
     CDS             36209..36652
                     /locus_tag="ACG8XC_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP9190029.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(36695..37504)
                     /gene="yidA"
                     /locus_tag="ACG8XC_000301"
     CDS             complement(36695..37504)
                     /gene="yidA"
                     /locus_tag="ACG8XC_000301"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP9190030.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(37523..38890)
                     /locus_tag="ACG8XC_000302"
     CDS             complement(37523..38890)
                     /locus_tag="ACG8XC_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9190031.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(38874..39704)
                     /locus_tag="ACG8XC_000303"
     CDS             complement(38874..39704)
                     /locus_tag="ACG8XC_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFP9190032.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            39867..40304
                     /locus_tag="ACG8XC_000304"
     CDS             39867..40304
                     /locus_tag="ACG8XC_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFP9190033.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            40380..41000
                     /gene="rpoE"
                     /locus_tag="ACG8XC_000305"
     CDS             40380..41000
                     /gene="rpoE"
                     /locus_tag="ACG8XC_000305"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFP9190034.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            41189..41695
                     /locus_tag="ACG8XC_000306"
     CDS             41189..41695
                     /locus_tag="ACG8XC_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFP9190035.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            41712..42611
                     /locus_tag="ACG8XC_000307"
     CDS             41712..42611
                     /locus_tag="ACG8XC_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFP9190036.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            42698..43583
                     /locus_tag="ACG8XC_000308"
                     /pseudo
     CDS             42698..43583
                     /locus_tag="ACG8XC_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(43775..43846)
                     /locus_tag="ACG8XC_000309"
     tRNA            complement(43775..43846)
                     /locus_tag="ACG8XC_000309"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43811..43813),aa:Arg,seq:ccg)
     gene            43906..45240
                     /gene="rpoN"
                     /locus_tag="ACG8XC_000310"
     CDS             43906..45240
                     /gene="rpoN"
                     /locus_tag="ACG8XC_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP9190037.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            45389..46480
                     /locus_tag="ACG8XC_000311"
     CDS             45389..46480
                     /locus_tag="ACG8XC_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFP9190038.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            46521..47522
                     /gene="gap"
                     /locus_tag="ACG8XC_000312"
     CDS             46521..47522
                     /gene="gap"
                     /locus_tag="ACG8XC_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9190039.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            47632..48822
                     /gene="pgk"
                     /locus_tag="ACG8XC_000313"
     CDS             47632..48822
                     /gene="pgk"
                     /locus_tag="ACG8XC_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP9190040.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            48926..49681
                     /gene="tpiA"
                     /locus_tag="ACG8XC_000314"
     CDS             48926..49681
                     /gene="tpiA"
                     /locus_tag="ACG8XC_000314"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP9190041.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            49797..51095
                     /gene="eno"
                     /locus_tag="ACG8XC_000315"
     CDS             49797..51095
                     /gene="eno"
                     /locus_tag="ACG8XC_000315"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP9190042.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        17058 a         8955 c        10858 g        14491 t
ORIGIN      
        1 tttatctttt caccttcggc agaagctgtt ggagcaacgc ctcctgccat ttcttatgat
       61 ccatacacgt atgaaaatat cgtacctcca aatggtggac gtagtgcagt caatggcggg
      121 aagcttgtcc aactaactgg ggctaaccaa ttgcctaaat tgactgctac ttatttgaac
      181 gatggttcaa atgatccttg gaaaggtggc acggtccgtt ttcattggga aagccatgtg
      241 aaactaacaa atgtacttga tttggtgaat atcactgcac aaggatttgc atttgtggcg
      301 atcatcaatt tgccggaaca tgtatcaagc agtgatgtat taaaagcaat tgactggaac
      361 aacgcctact tgtatattgc cggtgccccc atcaaggtta ctagcggtag tttattatca
      421 gcaggaagcc ataccttgcg ttttgggatg gggtcgtggg atacaaatac tctattggca
      481 gatattattg ctattattac gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt
      541 gatgttacag ctaatgtcgc gaagatgaca gaaaacggag ataaatatga tacgagtcca
      601 aacaaaattt tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc cgttttttct
      661 gttgattttt atgattcgaa taatattatc caagatcgta tgttcaattc gattttagct
      721 ccagctgcag gtagatttta tcctaaatta actggcggaa acttgaatcc aggagcatat
      781 gcattttctg caagtgcaga gataaatacg tggaatagtt acatctcaac aagtgattca
      841 acaggaaaat ataatacatt tgctgatacc acagaatacg tggatggaaa taaccagaac
      901 cttccaggct ctaccaatag tcggacattg acgatggggc tgactgaagg acgttttgat
      961 cagttgccat ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca
     1021 caaggagcaa tactgactca tactccagca agcacaagtg gattgaatca gacagccaat
     1081 atcgtgtaca gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta
     1141 ttggagcagt acaagctaga cggaacggtt ttaccaacaa acttagcagc cgatagtcag
     1201 tggggaaagc tcctttcttc tgcatcgtac caagtcacgg ccaattggtc agcaactgaa
     1261 ctaaaaaagg gttcgatcca ttacaggctt tattcgaaag gaacccaaaa attagcgaca
     1321 ggatggacag atcaactttt ccaaacgatc caatcaaata acgggggtgt aaatacagct
     1381 acttctacac tacctgcatt ggcttcaggg caatattatt ttgattatcg cttggtggac
     1441 gatgtactta ctaacatgta tccgtctttt gcgtacaaat ggcagtctga acaagctgga
     1501 atcaccactc ttcctcaaat aacagtggct aattttccat ctgtgagtgc agatagtcag
     1561 ctaaaaaacc ttagcagaca acctgagaca ggagatcccc tgtctgctct atcaggagat
     1621 acgattcaag aaaatgtgac attcacgtta acaaaatctg gggatgcgat aaccgacaaa
     1681 aaaatcacga tctcacttcc tgaaaatact acttatatca atggttcctt aaaattaaat
     1741 ggtacaacta tcgcagatac cggcattcaa caaggagttt ctgtcccagc agatctattg
     1801 agcaaaatag gagataccat tcatcttact tacaactatc aattaaatac tgtagatact
     1861 tctgtacaat ccgtcagcat actgacaaag gcagctgtac tttccagcaa tattacatta
     1921 gcagatggtg ctaagcttcc taacccagtt gtccaaacat ctgctaaaac aattcttgtc
     1981 cccaaacaag aattaacatt agttaatgta ccagatgatt ttacatttgg taacgatctc
     2041 cacaaaccgt tgaagacaag ttattatgaa gcaaaagtcg atttttcttt tgatgtgcgg
     2101 gatactcggc ttccaagcac gtctccttgg caactgacag ggacattgac ttctttattt
     2161 aagaatgatc aaggtcaaga attaagcgga acaaaattat acttcaatca ttctggatca
     2221 aaacaattga ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg aacagctaaa
     2281 ggagaggttc tcgttgactt tcccgatacc gatggtttac tcttagaggt aaatagcagt
     2341 acgaatgctc aacctggcgc aacgtatcaa ggcatggtca catgggaact gacagctggg
     2401 ccaaccagct aagtgattag aatttgtatg gatctgtgat aactgtcaca gatcctcttt
     2461 ttcaaggatg agaaggaaac ttctgctgtt aaaatcagaa atgcttggag gagtggactt
     2521 ggaacttttc agtttgcttg aaaaaaatga acaacggcaa atcaaacttt tccaactttt
     2581 attagaggaa aaaggagaga tttccgttaa taacgtactt acccgcttat cagttgatcg
     2641 ggctaccttg aaagaagatc tgttttattt acaagctaac ttggaaaata tcaaacaatt
     2701 ggaaattgtt atccaagaag ggaatatttt attaatcaga catggaaata tagcagcagt
     2761 cgatgtttat tatcattgct acttgcgcta ttctacaaaa tatcagatac ttctttatct
     2821 gttggaaaat ggctcatttg atcggcagaa acttgtcatg gaacttaata tcagttcggc
     2881 tactttgtct cgcagagtga aggaattaaa tgattcttta aatgagtttc atctgaaaat
     2941 aaaaagcggg cggctgaccg gtccagaaag tcaaatttgt tatttttatt tccaattatt
     3001 ttggtttgga cgtccttatc ttgaaaacaa aaaagaatac agtgatttat caggaaacac
     3061 ttttttagat ctgctgcaaa aagagtttag ttttactatt acagaagaag gacgattgaa
     3121 atttgaaata tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc
     3181 agattatagc aatcttcctg ttggattgtt tcaaacgaca caatttttat tggacctcca
     3241 tacatttttg acccgttact tctttcaata tgcctttcct tggaatcaaa atgaaacgtt
     3301 ggttttttat cttttcatga tcagtaactt tacattagat acacataact cgcatgtcca
     3361 ggagtttttg ataagcgaaa aatacaaaga cgacgtagta gctcaattga atcgaacgtt
     3421 taaacaatcg atagaagaat atttctcttt ttatgttttt ccagaagaga tttcgcaaaa
     3481 gattgcgttg acagtgatgc aaaatcacgc tcattttgcc tattttagag gatggatgaa
     3541 tatttttggc caagaaggat taaaacagcg gttaactgaa gtagaagata aacaattcct
     3601 ggttttatgt gaaagattgg tcagacaaac atttgctatt gttgatttta ctgaagtaga
     3661 cgaggaaaat gcccgctttg aattatttgc tcgctatgca tctattttgc ggacgctgat
     3721 caagcagtta gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc
     3781 ggatatcgtg aaagaaatca tccaggaaca attgctgaat acgatcaaat atgatttagt
     3841 cgaatacgag aaagaaaagc agtatgatat cattttgact agccaattga aagagtaccc
     3901 gtcacagaag tccgctaaag tttttgtatt cactagcaac gaatataatt atgatttccc
     3961 ctatttgaac caatttttaa aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa
     4021 aacatgaaaa tcgtgatcat tggtgcttcc catgcaggca tcacagctgc cctaaatcta
     4081 agaaaacttc aaccgaaagc agaagtttta ctgatcgatg aagatcataa agatggacta
     4141 ggctatgttt caaatggaat caacctttat ttaaaaggaa agatacgtag tttagctgaa
     4201 gttgcccata acatgcggac tcttcaagaa agtggggcaa aactgatcac cgaatggaga
     4261 gtaactgaac ttgatccaga caaacatcag ctcatcctcg catccaaaga aggaaaaaac
     4321 gagaccataa cctatgataa attaatcgta gcaacaggtt cctctcctgt caccttgtat
     4381 aaacaaattg aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc taagcaagta
     4441 cttgccgctt taaaagaagc aaaagaagta gtgatcttcg gtgctggtta tattggattg
     4501 gaattagcag atgctttgag aaataaagga tacattattc atctagttga ttatatgccg
     4561 aatgttttat caagatattt cgataaggat atgatcaaca gttttcaaaa tcagctgcaa
     4621 actaagcaaa tcaattttta tccaaatgaa ttcttgatcg attggaaaaa atcagaagag
     4681 aaagtagtat ccgtacaact tctatctcaa gcaataaagg cagatatggt cattttttct
     4741 gcccaaactc gtcctaatac gacattgtta aaagaaaaag tggctttgta tgaggatgag
     4801 actgtgatag tcaatgaata tctgcagaca agtgatccgg acatctatgc gattggagat
     4861 atcgtcccag tcagttttga caaaaataaa cggcatctct ttttaccttt agttactcga
     4921 gctgtacata tggcaagagc agtagcgcta actttgagtg gtcagccaac cgcttacgac
     4981 ctcagacaaa agataacagc agcagtcatt atagactact ttttaggaac tgtagggctg
     5041 acagaagatg aagcgccatt cttggaacaa aataccggtt cgtgttcagg agaatttgat
     5101 ctatttccac aatacgatga agaaaacaaa acagtaaatg caaagctgat ctaccatccg
     5161 gatacattag aaataattgg cggacagctg ataagccaag aattcttatt atcagatttg
     5221 aacctgcttg cggatatcgt gaaacacaaa actacgattc cacagctagc agttgaggat
     5281 tttggatttc tgtctgaaca cacctctcgt tttcactacc tcaatgagtt agcttttaaa
     5341 gtactagcta agagagcaaa tagcaataga gtagataaaa gactgtgaga aatatcacgg
     5401 tcttttattg tgtggggata taataaatat taatcaatcg agaataaaaa attcatataa
     5461 tgaaatagcg gaagaatttt ttgagcataa tattcgccaa aaatttatca agtgataaaa
     5521 ttgttgtata aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa
     5581 ttatgcattc aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc
     5641 tggtattttt tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg
     5701 acaagtggga ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt
     5761 ccatgattac taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc
     5821 agacacctgg agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct
     5881 tcttcggtat cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg
     5941 ataacgatga tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccgtag
     6001 gagatacagt taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc
     6061 atgccaaatt caatgtctca ccagttctct atacaaccat caatgcttac caagagatcc
     6121 gcttcgaaaa agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta
     6181 aaatcagtaa tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat
     6241 tacctggata taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta
     6301 ttgcggctat tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct
     6361 ttggtgtaat gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac
     6421 aaacattcat tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat
     6481 cccttgttct acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta
     6541 gcttgttgat gctggtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa
     6601 aaatcgatcc gttgaaggca attggataag aaggaggaag agcatgaaag caatagaatt
     6661 taaagaagta gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac
     6721 aaccatgagt atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa
     6781 aagtactttt ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat
     6841 caatgatcaa gaattcagta atcaaaaaga aagtgaacgc gttaaactac gttttaaaga
     6901 aattggcttt attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt
     6961 attattagta gataagatca aacatgaaca tcgtcaaaaa gaagcaaaag aattatttga
     7021 gcagcttggc gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg
     7081 gcaaagggtc gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga
     7141 accaactgct agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga
     7201 atcaaaagac aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa
     7261 atgcgataga atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag
     7321 caggtggaaa aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag
     7381 cgactgactt gtttatgtcc aagggatatc tcggcacttc aacaagagag atcgcacaaa
     7441 aagttgggat cacccaaccc aatctctatc attatttcgg tgataaagaa aaactatata
     7501 cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa
     7561 caagcgaaac gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac
     7621 atctggtcga tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa
     7681 gagatcatct tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt
     7741 cagcaaacca atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt
     7801 tcttagtgtt agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg
     7861 tggcgcaatt gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata
     7921 tagggagaaa aatagttagg tgaagaagca agatatgacg aagatcgtta tatcttgttt
     7981 tttttatata ggtagttttt cataaataca aatttgttat tctctcatga ttatcagggt
     8041 atatttttat ttattatgtc ggtgttccag gaagtgtttt aatttaataa cgaagaaatt
     8101 aattgtttgt agaatgaagg aaactattta ttgggtagat gaatcaccac gaatagtcaa
     8161 aaggagattt caggatatgc caaaaatgaa aaaaatcatt ttagcagtaa tgttattttc
     8221 tacggttata tcttttacag cccctttttt gcgtacttaa gctgatttta agttttgacc
     8281 cttagtctgg atctacgagg tgagagtcaa gaatgaaaaa aataattgga gcttttgtat
     8341 ttgcaatcat tatcattatt atctgttttc aaatagatgt ttttggtcaa aaaaagatag
     8401 ttggacaaac ggtcagcagt acgaacagta cggttacaaa gacaaaggaa tcatttgatg
     8461 atacagaaca agagacatta atcaaaatcg gaaaaaatga ccgtacacta tatacagatg
     8521 actcgctaac taccccactt gcaactataa acggcggaga gctggcggag tttctttctg
     8581 aaacaaaaaa tgcttatcag atcaagacaa atgatggcta tactggatat ttagccctcg
     8641 aagatggaac caaagtcaca aagaatatac aaacaaaacc ggaagagcta tctgatgcag
     8701 tgattgtatt agatcctggg catggcggaa atgatactgg ggcattaagc aatgatgaac
     8761 aaacagaaga aaaagatata acattaagta cagccataaa agtaaaaaag gcgctagagg
     8821 atgctggagc aacggtttat ttgacccata ctacagatga attagtacag cttggagata
     8881 tatgcgacta cagtgaagaa aaaaaggcag atgtctttat cagtttacac gcagactcta
     8941 cggaatatgc gaatgaagca actggaatca cgacatatta ttactatgga caagaggaaa
     9001 cattagcaca gacaattgct gactccttca ctgatctgcc tctggattca agaggaatct
     9061 ccactgggaa ctatcaagtt cttcgagaaa atcttcaacc ttctatttta atcgagatgg
     9121 gttatatgaa taatgatagt gatttaaagg aattagtaac agacagttat cagcagcaga
     9181 ttgctgcaag tttgacacaa gcattgacca cttattttca gtaataaata agcccagcca
     9241 aacaaaaata gctcctcata tttttcgttt ggccgggctt attatcgcag atgcaagctt
     9301 ttgaacaccg tttaaggaag aggttgtgag cctgccgttt aactccgcgt cacaaggagc
     9361 aatttcaaaa aacagcttct catgtttgat gactattgtg acttgtgacc gaggagttgg
     9421 catgagaaca ccgtttattc ggaacaaggg actgtgacat gacttttgtc acagttccat
     9481 tattttttta gcagcttcaa agctttttct gtattggcaa aatgaagttt ggcatatttt
     9541 tcaagcatag gcatgccgcc tttctccaat agttttgccg ctacttggtc agatttcgtt
     9601 ccagctcctg atggaagggt gattccagct tcagctgatg ctttgtgtaa ctcttccaaa
     9661 aagctggcac gactgatgat agaagcagca gcaacagcta aatgatattg ttctccttta
     9721 gtaacgaagt acagtttttc ttgtaattga tttttttcta aacgcgcata ttttttaaaa
     9781 tttgcttccg gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg ctcgattttt
     9841 tttagcagca aatggatcgc ttgattgtgc aaagcgactt tcatccgaac ggcattgtat
     9901 tttggttgga tctcattgta tttttctgga gataagacaa gcagtttata ggggatggtt
     9961 ttttttaaga tgtcagcaag ctggatgatt tggctatctt ttaactcttt tgaatctcta
    10021 acaccaagtt tacgtaattc tgcaagctga tcacgtttca cataagcagc acatactgtt
    10081 agcggaccaa aatagctgcc atttccgacc tcatcactac ctaaaacgga taattgactg
    10141 atattttttg gcaaagaaga ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc
    10201 ccccatttat cagcttctat tgctgcattc gtaccttgaa acatcacttt tccggatgta
    10261 taagcggtaa tggtcgtccc gtcttttttt gcagcaaagc tagtataagg aatctgttta
    10321 tttaacaaat aagagcgata gtatgctttc atttgactaa gttggtcgtt tgttgctttg
    10381 ataatgacgt tgctcataaa attctcctcg tttttataca taaaactata ccgcaactca
    10441 cgacttttcg ttttctaaga gattgcgaga catagcattt ttttaaagtt tatcatataa
    10501 tagttaagta tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt catggtacac
    10561 taaataaaac tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct
    10621 acaaagcagt gattgccaac caaacatata ctattatcgg acgggaaacc aagcatcata
    10681 tggatatcgt aacgaagctg atcaatgaac agttagcaga attgaaacaa ttatcaccac
    10741 aaatggataa tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg gatcaattga
    10801 agaaacaaga aagaatcttg gagctggaag aagaaacagc agaattaaag aaaaaaatga
    10861 tcaaatttac agagcttgaa aatcgtgtga aacgcattga agcaatcgag aacgaagctc
    10921 gggaagtatt gaaagaaaat ggtcaagcag atcataccat taaaaatcat gtggaagcac
    10981 aacagatttt aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct aattaagatc
    11041 cttttatcaa aataagaaaa atttaaaaaa gttagaagag aaaggaacgt agcatgctga
    11101 gtttgctgat tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg
    11161 ctttgcaaat catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt
    11221 acaaaccatt agcaagtcat ttggaattat acattccata cccagctgtc accccaacat
    11281 caaagttagc attttttgat caggtatttg cttttcacct agatgaagct ttttatgcag
    11341 gaactgcttt tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt
    11401 ttgtccatgg attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca
    11461 tcctgtcact aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg
    11521 ttccattaga tgtggtccaa aatcaattcg gcgagcatag tttcgctcgt tttatcgttg
    11581 agagaacgcc atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt
    11641 aaggaaaata gtgctggaaa atatttcgtt ttttgtacag agtagtttat ttaagaaggc
    11701 tgcgtagcgt atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga
    11761 ataaccgaat ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg
    11821 tagtgactgc acaaggaaaa gaagaactgg cagaattggt accagtgagt gagaaacaaa
    11881 cgatcacaaa ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg
    11941 ggatccctat acctaaactt gaaaatatcc gtccccatat gaaacggatc gagatcggag
    12001 cagacttgaa tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat
    12061 tgaatcgttt cattgatgat ctttctgaca gtgaaatcga atttgcacgt ttgtatatgt
    12121 gggcagatca gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg
    12181 aagacggacg cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc
    12241 ggcgaagtga acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa
    12301 agtatttaag tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac
    12361 aagaataccg cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggacagactt
    12421 tatttattga accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg
    12481 ctgaacgaaa tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc
    12541 gtcaagaaat catccacaat gcttatgtga ttggtaaaat ggatctgatg aatgcaaaag
    12601 cacgatttgg taaagaagta aaagccatcg tgccgggaat cagtgaagac aatcatgtcg
    12661 tcttgaaaca agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca
    12721 caatcggaaa agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga
    12781 tcacgttgaa gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg
    12841 caggtgaaga aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac
    12901 agtcgattga gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt
    12961 tatcaaaagt caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc
    13021 cgcaagaagg tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt
    13081 atgtgatggc aacaacgcat tatcctgaat tgaaagttta tggatacaat cgggcaaata
    13141 cgattaatgc cagcatggag ttcgatgtgg atacactaag tccaacgtat cgtttgctga
    13201 ttggcgttcc tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag
    13261 aagttattga tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga
    13321 tcacagattt agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt
    13381 ttgtttctga ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg
    13441 aagaacgtga aaaagaaatg gaaaaagcaa agaaaaaagc aaacgaagtc gtgtcagagg
    13501 cggaagaaaa agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc
    13561 aaggaaatgt gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc
    13621 aagaagaaac tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga
    13681 aaccgggaga cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa
    13741 acgggaatca gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc
    13801 tgacgcctgt tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg
    13861 aatctagtag tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc
    13921 taagtgaggt agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta
    13981 ttgtccacgg aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc
    14041 atcggagcgt caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa
    14101 ttgttaaatt taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga
    14161 aatgctatac taatcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac
    14221 agataagaca tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac
    14281 ttggtgtggt ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga
    14341 tgaagaagaa ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca
    14401 attcggtatc atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa
    14461 agctgttggt gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa
    14521 acttacagca aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg
    14581 cggttgagtt aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg
    14641 attgtttttt tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga
    14701 tgaacgaacg aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga
    14761 tgaaagataa aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg
    14821 tccgctcata ttttactggc agccataata cgaaaacgga acggcttgtt agtgaaatcg
    14881 aagactttga atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc
    14941 tgataaagaa aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt
    15001 tccttaagat aacgaatgaa aaatatcccc gtttggtgat tactcgtaaa gtactaaaag
    15061 acaaagcact gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga
    15121 tcctcgatcg attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc
    15181 tttactatca tttgggacaa tgtctgtgtc cttattattt cgacgtagat ccgaaagtct
    15241 atacgaaaat cgttgatgaa gtgaaaaagt ttttaaacgg aggacacgaa aaaatcgagt
    15301 cagagatcca tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag
    15361 aataccgaga ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata
    15421 ctgatttact ggatcgagat gtatttggtt atgcgattga caaaggatgg atgtgtgtcc
    15481 aagtgttttt cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct
    15541 ataacgaagc agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc
    15601 atttcattcc taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt
    15661 tgttagctac gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg
    15721 cgaataagaa tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg
    15781 aacggacgat cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac
    15841 gtatcgagtc ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg
    15901 tattcattga tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca
    15961 acggaccgga tgattatgca tcaatgagag aagtcatcta tcgcagatat tcacgagtga
    16021 ttcgcgaagg gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag
    16081 atgtggcaaa agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg
    16141 caaaaaacga taaacataaa acaaatgagt tgctattcgg tatagatttg gctgtagtac
    16201 cgcttgagcg aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc
    16261 gattcgcaat cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag
    16321 atgaaattga agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt
    16381 tgaaaaatat cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg
    16441 tcgcacaaaa cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa
    16501 agtaaagtgt cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat
    16561 aataaatcgc ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag
    16621 aaatgttaga agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg
    16681 tctctttcca aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga
    16741 agacaacgac ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat
    16801 ggaatggcca tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac
    16861 ggggacttta tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag
    16921 ggaaatcaag aaaagagatt gaaccaaaaa tcgatttttg gatggaaaaa tttcaggtaa
    16981 aagggaaaaa gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc
    17041 tgatcgccac gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt
    17101 tagatccggt aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt
    17161 catgtgtcat tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga
    17221 tcatgttgcg aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat
    17281 ttggccgtac gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg
    17341 aaggcgtgaa aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc
    17401 ctgaagctgg gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta
    17461 accagcaacc gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt
    17521 aagttttgga ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg
    17581 atcatgatat tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct
    17641 caacaaaata gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag
    17701 caaatgagcc aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa
    17761 gcacaaaaag agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat
    17821 gataagataa atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg
    17881 atacaacaac aactgactgg cttgcaatcg atgcttcgag caagtcaatt aggcatttca
    17941 ccagaagaag tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt
    18001 gattcaaaag gaaaaatgac agttggagaa gataactcga cagtccaata tgtcgtaagt
    18061 tatgtagcaa cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa
    18121 gagatcgctt ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca
    18181 caaaagcatt tttacggaaa actaacaggt gttttacttg ttgcattaac gcaaatggca
    18241 ttatacggta ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa
    18301 aatatattag acggtatctc attggataac ttgattggac cattcttgtg gtattctctt
    18361 ctattcatga tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg
    18421 gtcaacaaag cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc
    18481 ggatatatgc ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc
    18541 acttcgtata ttccattctt atcttcatat attatgccgg ttcgcttagc aaatgagaca
    18601 gtcggtatgg gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg
    18661 atgatcttgt ctgcaaacat gtacaaatcg aatgtactcg tgtatagcga aggcggatta
    18721 tggagttcat tgaaacaatc gatttcaatt atgagaaatg aacgaaaaaa agcataaaat
    18781 atagaaaaat atagaaggaa gctggacatt tattgttccc agcttctttt ttgtatagac
    18841 ctatttctat ttttttatta tattggatat atattcattg aaaatagagc ataacaactt
    18901 tataatgttg agtataccaa tttttggagg cgttttaatg gaccgctatt tagatttact
    18961 ttcagagaag ttccctacaa cggaagatgt catcacggaa atcatcaatc tcgaagcaat
    19021 tttgcagctt ccaaaaggaa cagagttatt tttgagcgat atacatggcg aatttcctgc
    19081 ttttgatcat attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt
    19141 ctctgaacaa atgacggaaa cggagatcag ccaattcact atttttacgg cttatccaga
    19201 atatgtgttg acgactgatt ggtacaaaaa acaagataaa tcccagctga tccatcgttt
    19261 gatcgatctt ttacggttta cgacagtcaa atacactcga tcaaaagttc gaaaaggatt
    19321 gccgaaagaa tatagctata tcattgagga actcttgtat atcgatgatc gtatcaacgg
    19381 gaaaaaagac tatgtaaaaa aaatcattga acaacttgtc ttgatgaagg aagaagaaaa
    19441 atttttaaca aaacttgcgc agacgatcca aaaatctgtc atcgatcatt tgcatatcgt
    19501 gggtgatatt tttgatcgag gaactcaagc agccaaagta atggatcgaa tcatggattt
    19561 ttcctctgta gatatccagt gggggaatca tgatattttg tggattggtg cgtattgcgg
    19621 ctctgaggca tgtttattga cgcttttacg gattgccgca cgctacaact atttatttga
    19681 attagagaaa gaatatggat taaatttacg tccattattt tcttttgccc atcaaaccta
    19741 tcagaagaat ccagtattta cccctaaaaa caggaaagat ctatctgaaa gagagcaaga
    19801 agaattagaa aagatccatc aagcactttc tatcctccaa ttcaaatcag aacatcagtt
    19861 gttgaatcga cgacctgaat ttaaaatgga cgatcgtaaa ttgttggaaa aaatcaacta
    19921 tgaggaatca acgatcgttt tagaaggtca gctctatgag ttgaaaggga cttgttttca
    19981 aacgatccag ccagatgatc ctaaaaaatt cttggaagag gaacaaaaag tgatcgatag
    20041 tttgatgtat tcattccagc actcgttaag aatgcaaaaa cacatgacgt ttttgatcga
    20101 aaaaggggga atgtatctca cgaacaatca gcatgtactg ttccatggct gtatacctgt
    20161 agatgaaaag gggcagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact
    20221 gctgggattt tttgaaaaac agataactga aagcgcaaaa gatttgactg caaatgaaga
    20281 tttggcgacc gatttgattt ggtatgcgtg gagcgggccg ttttcgcctt tattcggcaa
    20341 gagtaaaatg gcaacttttg agcgctattt tctcacagag cctcatacgc acgtcgaaaa
    20401 aagcaatccc tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttggcaga
    20461 attcgatgca aaagaagaag gttcagcaat catcaatgga cacacgccag taaaagtaaa
    20521 aaaaggagag tcaccgatca aagcaaacgg aaaagcattt gttattgacg gaggtctatc
    20581 caaagcttat caaaaaacaa ccgggattgc tggctactca ttactatgca attcttacgg
    20641 tttccaaatc gtgacacatt atccgttctt gcctattgaa caacaatttg caaaaaaatc
    20701 tgatcaaacg aatgtaaaaa aagtgatcga gcagcagctc ccacgcaaac ttaatcggaa
    20761 tacgacaaaa ggaatggaat tacagcaaca gattactcaa ctccaaaggc tgttggatta
    20821 tcgaaaggac gattgagaaa aatgctagcc aacattgctc aatagactaa aaagcgactg
    20881 caacaaaagg cgtgtcgcag tctccttcgg aattagctca aaaaacggta aaatattaga
    20941 agctattttt ctgtttattt ctctaatact caggtcaggg cgctgatttc acgacctctt
    21001 ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc aacaaacatg
    21061 agaagctgtt ttttgaaatt gctccttgtg actcagagtt gaacggcagg ctcacgacct
    21121 aatcagaaaa acgattaaaa ttcattcaca aagaaatcga ataaacgctg ttcagatgaa
    21181 tctacacgat gagaaagttc cggatgccat tggacaccaa ggattttgtg catattatct
    21241 ttactttgga ctgcttcaat gagtccatcg ttggaaaagg cgatggcttt taacaaagga
    21301 gaaagatctt tgactgcctg atgatgatag gaattcacaa agtatgaatc agggagaagg
    21361 tcacttaata aactgtccga aacgattttg acttcgtgag ttgcaaactg cggggcagtg
    21421 ggctgttgtt cgtgttttac ggaccatttt ggatataatg aaagatcttg ataaagtgtt
    21481 ccctccaaca cgacattcaa tagttgaagt cctcggcaga ctgcaaaaat cggttttctt
    21541 tgtttcaatg cttccaagat caatgcctgt tcgaattggt ctcgttgaat atttgtttcc
    21601 gtcagttttg gatgagggtc ttccatgtaa agttgcggac taacatcctg accgcctgca
    21661 agaatgagtt tgtcgatttt ctcaattagt tgaggcgctg aatctggaga catgactggc
    21721 agaataagag gtaatccttg agcatctaaa actgcatcca caaatccctg cggtgtatag
    21781 cttacttgat ttccttggaa agtatctgtc gctcgaatca gttgattccc ggcaatgccg
    21841 atgattggtt tcatttttat tccccctaaa gtagactaat tgtaacaagg atttaaaaaa
    21901 atttaaacta tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta
    21961 aagagaattt tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa
    22021 taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg
    22081 ggtgagaagg acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat
    22141 actttcaatg aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag
    22201 tgttagaagc tttggaacaa atccgaaaag cagaagaaga aaatgagaag cgtgctcgaa
    22261 ccctacggca gcagttgaag acctatgcag aagaaaaaga gcaagaattg gagataatcc
    22321 aaacgaacag tcggcaacag ctgaaacagt tggtagaaga aaaagaacaa ttcgaaaagc
    22381 aagaattaga aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt
    22441 tgaaagacca atatcaaaag catcagagtc aagcaatcgg tgcaatcatt gaaagggtga
    22501 aagaaactta tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat
    22561 caggagagga ttttacaagc agtacaagga atccaaaatg ttgaaatccg ggatctgtat
    22621 caaaatagaa caaacaacca gtgggtagaa aaatattttc cagataccac gattattgat
    22681 aaagaagcaa aattaagcgc tttaggcaat cggctgaaag acatccaaga agctatccaa
    22741 ttcatacaac accacggcga tagccgtcaa aagaaactcc atttgaaacg tcaagaactt
    22801 tccttacaag acttggaagc aaattattcc gaagaaaatt tcatgaataa attagaagaa
    22861 gtccttgatt taaaagaaaa gtgggaacag ctaactgaaa aacgtgatcg tttagaagaa
    22921 gaggaagaat ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt
    22981 aagacgacca tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa
    23041 gaactaagta agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt
    23101 tatctttcgt atgttgcttt aaaaaaatca gttgctgcta tagaagaaat tgctgggaaa
    23161 tacgggttca ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa attgcaagaa
    23221 accaaacagc aattatccat tgttcacgag cagcaaaaaa agataacgac agcgttaggt
    23281 cagtgcagcg gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca
    23341 gaacgggaaa aagtaaaaga acggtttatc catgccactt atctgatcgt tttgcaaggt
    23401 tgggtagatg cagaagaaaa acagaatttg atgcatgtgt taaacgaaac tgtatcagaa
    23461 gaagatatat acgttagttt cgaaacacca aactcagaag agattcagca ggaagtccct
    23521 actaaattga aaaaccatcc gcttatcgaa ccttttgaat tgctgactga aatgtacagt
    23581 ctgccaaaat atgaagaagt agatccaacg ccttggatga caccatttta tcttgtcttc
    23641 ttcgggatga tggtcgcaga tgttggttac ggattgctga tgctcattgg gacaatacta
    23701 gctcaaaaac tatttgtttt accccgcggg atgaaacgtt tcgtaaaatt ctttgagatt
    23761 ctctcggtac catcgatcat ctggggattg atctacagtt catttttcgg acaggcatta
    23821 ccaaaagaac tatttggtat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc
    23881 aatacgattt tgattttatc cgtgattttc ggtctgatac agatcttggt tggattattc
    23941 gtggcagcaa aggaaaatat caggaggaaa gattacttga gtgctgtcag tgatggtttt
    24001 gcctggcaag ggatcctgat tgggatcgtt attgcattga ttggtgtcat gttgataaaa
    24061 aatacagcat ttgtttatct tggcgcgggg ctatcgatca ttgctgcttt atgtatctta
    24121 atcgtgccga ttatccaatc gccgtctaaa gtcaagggtg ctgccaaagg agcgtataat
    24181 ctttacggat tgactggtta tatcggtgat ttagtcagtt atacacgttt gatggcacta
    24241 ggtatttcag gcggaagtat tggcgcggca ttcaatatgc tagttgcttt catgccgcca
    24301 gccgctcgat tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt
    24361 ttgacactgt taagtgccta tgtccatggt gcacgattgc agtatgtcga attttttgga
    24421 aaattttata caggaggcgg acgtgccttt gacccgctga aaacggcgga aaaatacgtc
    24481 aacatcaatc ataagaaaaa aaacaataaa taattggagg atttttatac aatgatggat
    24541 tacttaatta gtcaaaatgg tggaatggta tttgcagtat tagcgatggc aacagcgacg
    24601 attttttcag ggattggttc agctaaaggt gttggaatga caggtgaggc agcagcagcg
    24661 ttaacaacaa gtcaacccga aaaattcgga caggctttga ttctgcaatt gcttccaggt
    24721 acgcaagggt tgtatggatt cgttatcgcc tttttgattt ttatcaactt aggaaatgat
    24781 atgtcagttg tacaaggatt gaactttcta ggcgcttctc tgccgattgc ttttacaggt
    24841 ttgttttcag gtatcgccca agggaaagtt gcagcagcag gtatccaaat tttagcgaaa
    24901 aaacctgaac atgctacaaa aggaatcatt tttgctgcga tggttgaaac atatgcgatt
    24961 ttaggtttcg ttatttcctt cctattagta ttgaatgcct aagcataaag gaggaaaagc
    25021 agatgaacgc cattgaaaaa atcatctctc agatgaatga agcagcagag caagaaagag
    25081 cggcgttaga gcaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc
    25141 aaatcgaaac agaacatcaa aaacaaaaag aaaaacaaat cgaattactt gaaaaaaaat
    25201 accgtcagct taggaatcgc caacaagtag aagtacgaca agaaaatttg aatgcgaagc
    25261 aggaattttt acgacgattg tttgcagatg caatagctga aatggagaat tgggatgaat
    25321 ctgaacaaat ccagtttatc aaaaattcat tgtattcatt acctttgaca ggcaaagtaa
    25381 cattcattgc tggagaaaaa tcagcagctt atctttctca gacattgctg gatgaatgga
    25441 acaatgaact tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt
    25501 ttctgataaa cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata
    25561 tccaaggaac gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg
    25621 tatggaatac cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa
    25681 tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa
    25741 atccacgatt tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct
    25801 tttaaaggag cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat
    25861 cgtttggatc tatacgatgc gctatacttt ccataatctg aaggttttga caaaagccga
    25921 gatgacaaat caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac
    25981 cttgaaggat gccattcata cgcaagtttc aaatgaactc cctgaaagca ttatggacag
    26041 tatacgagaa gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat
    26101 ttatgatcga cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga
    26161 attgctgaca gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg
    26221 aatattgcag aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat
    26281 cgaaaaagaa gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct
    26341 attagagaca gattatgctg attttctgcg ccctgtcatc cgtgagggaa atcttgatct
    26401 cgtaaagctt gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac
    26461 ccaggctttc ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa
    26521 aaaccttcgt ttactggtta tcggcaaaag aagccatttc gaaccagaaa caatcagaaa
    26581 gcgggtgaga caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt
    26641 tgccattcaa attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc
    26701 aggcaattga agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg
    26761 cagcattgag cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat
    26821 tgattcccaa tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag
    26881 aaaaagcagt aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg
    26941 gaaaaatcat aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta
    27001 tccaagacat gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg
    27061 gcgatgtggc gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg
    27121 ttcgctctac tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt
    27181 ttgacggaat ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag
    27241 ggcgcggagt tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag
    27301 tagaagccgg agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag
    27361 ttataaaaca taaaatcatg gtgccaaatg ggataaaagg tacgatcaaa acaatcgaaa
    27421 aaggttcatt cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa
    27481 atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa
    27541 acccggatgc acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa
    27601 aaggaggagc agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc
    27661 aaattgcaaa atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa
    27721 atgagatgac ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat
    27781 cgttgatgga acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag
    27841 aagcttctat ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg
    27901 ttgctatcat ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac
    27961 gtttggaaga aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg
    28021 aatactatga acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta
    28081 taaccgcaat cagtgctgta tcgccgtctg gaggggatat ttcggaacca gtcacacaaa
    28141 atacattgcg ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac
    28201 attttccttc gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt
    28261 atatggatca tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt
    28321 tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag
    28381 ataatgaccg tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa
    28441 atgcttttga tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga
    28501 tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg
    28561 agatcatgga cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg
    28621 aaacagaatt agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga
    28681 tcgtttcaga aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc
    28741 ggtccattga tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc
    28801 cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca
    28861 atggtccaaa tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt
    28921 ttaggccatc ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt
    28981 ttgggacgac cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac
    29041 ggagaagtaa tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc
    29101 tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc
    29161 tctggtcttc ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat
    29221 tcatcagatg atttcgccgt agtctttgct gctattggaa tcacttttga agaagcagaa
    29281 tttttcatgg aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat
    29341 ttagcaaacg accctgcaat cgagcgtatc gctactcctc gtatggcttt gactgctgca
    29401 gaatatcttg cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac
    29461 tatgcagagg cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga
    29521 tatcctgggt atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc
    29581 ggactaaaag gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact
    29641 catccaatcc ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag
    29701 ctttacaaga gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa
    29761 gataagggaa ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt
    29821 tttgcagcct atgcacaagg aaaacaggca aaagaattgg ccgtagtttt aggtgaatct
    29881 gctttatctg atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat
    29941 gtcaatcaag gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa
    30001 ctgctatcga tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa
    30061 tatctgccag aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg
    30121 gaattgactc gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa
    30181 gataagcaag atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg
    30241 cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct
    30301 tcgatcacgg agcctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg
    30361 tctatcgtag acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat
    30421 gaacagctga cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat
    30481 cggtcaatcg atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa
    30541 aaaacttgtc aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta
    30601 gaatatatga cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa
    30661 gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac
    30721 taagtggtat ccttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga
    30781 aacgcttgcc ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg
    30841 gcggcctact taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg
    30901 cgcttttcac tgcaacttct gctgtctgtg tgacgggatt gacaacattg aatacagcag
    30961 agcattggaa cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg
    31021 gttttatgat ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca
    31081 cacgtattat attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca
    31141 tgctttacat cctgaaattg gcctttgtga tccaactgat tggtacggtt gctttatctt
    31201 ttgtattcgt gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa
    31261 tctctagttt ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc
    31321 aaaatgttta tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca
    31381 tcgtatggcg tgatttactg gaatatcatc agaaaaagaa gataacgatt cattcaaaaa
    31441 tcgcattaac cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag
    31501 agcgcaatgg ggaaacgcta gtggaaggga atatgtttca acgattggca aacacctttt
    31561 ttatgagtgt gacacctaga acggctggat attattcagt tgattacctc catatgagcc
    31621 atgcaggtct gattttaacg atgtttttga tgtacatcgg tggaacatct gggtcgacgg
    31681 ccggcggatt gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgctta
    31741 aaggacgtac gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg
    31801 cacttacttt attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg
    31861 tgactgaaac gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg
    31921 cgtttggtac agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat
    31981 tgatcattat agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc
    32041 tgatgacaaa agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga
    32101 ttggataatt tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata
    32161 atttttcttg cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta
    32221 tccgttgttg aacctcgggt agtgcctctt tcttattgaa aggaatcaat aagatttgat
    32281 tcctttattt atcaataggt tataattcat atttttttat tttttctttt acgatactta
    32341 gtccagaagc taaaatttca aggttattag aggataggga aattcgaacg aatgcatctg
    32401 gttgcgcacg tgtagtaaag cgtctggaat gataaagtcg gataccgtta gcaagaaagt
    32461 cattctcaat tatcttagga tcaatctcag gatcaactgg aagaattcga tagtaaggga
    32521 cacgattcgt tggtttttct actttgaaaa gagaatcaaa aagctgattg gctcttttcg
    32581 taagacttaa tttagtttct aagatcctat ttgcttcatt tgaatggagg atttgagtga
    32641 tgacttccac atctaatcca gaagttttca catttatatt aaaaattgct tgttctatct
    32701 tgttttggac atgtactgga actacaagat aagcaactcg caatccagaa caaataaatt
    32761 ttgtcattcc agctaagtag attgtttggt taggtaataa tttttggaaa ggaggaatga
    32821 tcgtatgttg atagtaagtt gttaaaaatg aatggatatc atcttcaata acccatagac
    32881 cttctttttc aatcacttcg gctaattgct ctcgtctttt gtgagagatt tgaaacccta
    32941 taggattatt acaagaaggc ataagaaaga caccatgaat cttctttttt ctacattcct
    33001 gaatcaatat atcaggtcgc attccttgct cgtcaaaatc aattggaacg atctctaaat
    33061 gatataactg agccaattca atgaaattag cgtatgtata tcggtctgtt gctattctat
    33121 cacctggaga gaaaagtgca gaaagtgtga tagccaatcc attttgaaca ccggaaacaa
    33181 tagatagaga ttctaaggac ggaatgactc cttgagtttt taaccaattt gctgcagtag
    33241 tcaattgaaa atttgtgcct ttgggatctt ggtaattaag caaaccaaca agatttgggt
    33301 tattactagt tgattgaata aacgggacaa cgaatttatt acattcttca aaagaactaa
    33361 ccagaccaaa atcaatgata tgttcggtta cttcattggt agagatggtg atggactcaa
    33421 agacattggg agaaataaat gtgccacttc caatattcgt atacagcaaa cctttctcaa
    33481 ttccatattt ataagccttg ccaacggtag taaaatttaa atctaaataa tcagctagct
    33541 ctctttggga gggtaactga gtgtttttct gtaatttatt ctttaaaata tcttgttcta
    33601 attgttgaat caacgatcga taataaggac gcttcaattt ttgtttgtct ggtcgccaag
    33661 ttaacgggta attgtcataa gaattaatag gcattttctc cactccttaa aattgtatac
    33721 catacaattt taccattgat tgtattgatt ttagaagtta atcttttgtg aggagggaat
    33781 tgtctatgaa aattattaat tttgaggaag tagtagcttc ttttagtttt gaagaagcga
    33841 ttcaagtaat gaaaaaatgt ttttttgatt atgaaagtgg taaaatcagc caaaatcctc
    33901 gattggtgga aattttacct gacggaaaag acaaaaatat gtttgcgatg atgcctgctt
    33961 atttggggga gaacagatat tttggaacaa aagtcattac cgcttttccg gataatcatc
    34021 agtatcattt accttctcat ttaggagaga ttttcttgtt tgatgctcaa aacggactac
    34081 ccaaagcatt gataaacgca aatgcagtca cctggattcg aacagctgct gtatctgcgc
    34141 tggcaacaga ttacttagca aatccgcatg cagaaacact tgctttaatt ggagctggtc
    34201 aacaagcatt gtcgcatttg actgcgattt ctaccattcg tcccatcact acagtgtttg
    34261 tttacgatat tttagaagaa agacgaaata cttttatcgc taaagctaaa aaacagtatc
    34321 ctaaaataac gtttataaat gcaatatcag tagaagatgc agtacgtcca gcggaaatca
    34381 tttgtacgtt gacctctagt aaagaggcat ttcttcaaaa agagtgggta agggaagacg
    34441 cacatatcaa tgctgtgggc acatttactc ctgatacaag agagataagt acatcgttga
    34501 tggaaacttc tcaaatctat gtagatgatt actcagctgc cttaacagaa agcggggacc
    34561 tgcttatagc catggcagaa ggagatttag aggaatcttg tattgttggt acattaggcg
    34621 aattagtttc tgagaaaaaa acattttata aaggagaatc gaggccgacg attttcgacg
    34681 cagttggtct agctattgaa gatctttgtt gtgcagaata tatttataac aaattagaga
    34741 aaggggcttt aaaatgaact tagattatta cgtagttgat gcatttgcag atgaggtgtt
    34801 caaaggaaat ccagcagcgg tttatgtgct agaagaatgg ctgccagaag gtacgatgca
    34861 aaaaattgcc attgaaaaca atttatctga aacagcattt acagtgaaga aaaaccaaga
    34921 atttgagctt agatggttta cgccagatcg agaaatcgat ctttgtggtc acgcgacatt
    34981 agcaacagca tttgttttat ttaactatta caaaatacct gatgaaacga ttaaattttc
    35041 tactcaaagc ggtaatctat ttgtgacaag aattaaagat tattattaca tggattttcc
    35101 tagtattatg ccgaaaaaag tgccgatatt gactgaatat gaagaagcaa ttggggctaa
    35161 gattaaagaa gcctacttag cacgtgattt atttttcgta ttagaagatg aaaaaacggt
    35221 ggctaaattg cagccagatt ttacagctat caaaaatttt gaattaggaa tagagtcatc
    35281 gtgacagcag aaagtgtgca aaaagacttt gtttctagaa ccttctttcc aaaattaaca
    35341 atcaatgaag atccagtatg tggatcagca cattctaatc ttattcctta ttgggcagaa
    35401 agaatgaata aaacaaaatt gtcagcttat caaatgtcat caaggggcgg ggatctagaa
    35461 tgtgaattaa aagaagatcg tgtgataata ggaggaaaag cagttttgtt ttctaaaggt
    35521 acctcgttta tttgatttct ttcaaatgaa ttaaaaataa attggtggag agtggcagcc
    35581 ttatagctac aatggttgtt aaaaaaagac gcaaactttt tcaatttaat gaaaagtttg
    35641 cgtctttttt taattattgg tgtttgtttt ttattcaatc aagagtaaaa tgccccatac
    35701 taactatcct cggatgactt cgattttata accatcaggg tcaatgacaa agtagtaaga
    35761 aggcgtagtg ccaggtaatc cttttaagtc agtaatctca agacctgcag ctttatgttg
    35821 ctcgtgtaat ttttctaaat catcagctgc aatagcgata tgaccatagc cgtctcctag
    35881 atcatacgca ccatgatcat agttgtaagt caactctaat tcatagtcat ctccaggaag
    35941 tgtcaaataa acaagtgtaa atttgttttc tggaaaatca cggcggcggc tttcttcaaa
    36001 accaaatgcc ttttgataaa attcgattga tgcttcaaga tctttcaccc gaacacatgt
    36061 gtgagccatt ttcataaatt catctctcct tttcctttta ctcaatcata acaaaaagaa
    36121 gtaagaaaat agttgatttg tgcttgttca tgagaaattt ctcatgtaaa atggatagaa
    36181 aataatactg ttaaggtgga aacaattcat ggataaacca atttttggtg aaaaaaagca
    36241 atctgctaat tatcaaaccc gttatgcagc atatattgtc atagaaagag acaagcggat
    36301 tgcggtgatc gaggctccaa atggtgcttt tttccttcct ggtggggaaa tcgaaggaga
    36361 agagacgaaa gaagaagcga ttgtccgtga gctgatagaa gaaatgggaa ttgtggcaga
    36421 aatttcttct tatctgggag aagctgatga gtatttttac tcgaattatc gtgcaactta
    36481 ttattataat ccaggatatt tctttgttgc tgattcttgg aagcgtatag gagaaccaac
    36541 tgaaaagacc aataaaattt ggtgggtgag ccctcaagaa gcgcttgtga aattgaaacg
    36601 tggtagccac cgatgggcag tcgagagatg gatcgaaaaa aataaaaact gaaagaaagc
    36661 gagtagatca gctttctctc agttttttta tttattacag tacatatttt ttgattgctt
    36721 cagctacacc gtgctcgtcg tttgatgaag tgatgacatt tgctgcttct ttgacaagag
    36781 aaacggcatt tcccattgct actcctagac cagcatattc aatcatggat aagtcgttct
    36841 cgttatctcc gatagccatg atttcatctt gatcaatacc aaggtgctgt gcgagattcg
    36901 caactgccgc acctttactt gcttctttat ttagtacttc gaagtaatag ggcgcacttt
    36961 tgaccgttgt gtatttatca cggaaagttt gcggtaaacg cgcaattgct gcatctaaaa
    37021 tttctggttc gtcgatcatc atcattttga cgatgttcat gtctggtgtc atttcttcag
    37081 gtgttcgata ttttagcggc atcttgacta aacttgcttc gtggatcgta taagcgctga
    37141 tatctcgatt aggcgtgtag atcgctttgt catcgatcga atggagatga ctgccgacac
    37201 ggcgtgccat cacttcgatc tctaggaaat cttcatgagt cagtccgtga cgggcgatga
    37261 ttttacctga tttcgtttgt tgtacgagtg ccccgttata agtaataacg taatcattat
    37321 cttgaaaaag gttcaactcg tttaactgtt cctggacacc tggtaaggga cggccagtgc
    37381 ataaaacgat cgaaacacct tttgcaacag cagcggcaat cgcttctttt acttcttttg
    37441 ttacttctct tttctcattt aataaagtac catcgatgtc gatagcgact agtttgatag
    37501 acataatttc ctccaatata ttttaattta tttcaatcaa tgcgccatta cggatatggc
    37561 tggcaaattc ttgataagtt tcgtcaaata aatcataatg atcacgcaag gagggatcaa
    37621 ccatttcttt tggaaagaaa aatcgctcat ctccttgttc ttgtcccgct aatgcagcaa
    37681 caagctgact aacctgtgat aattctatca aagttccgtc atttcgcaaa atctcgattt
    37741 gtgtgcgatg tctgccttgt tctgggcgat aaaaatcata aggcagatcg tagctagaat
    37801 tcacagcagt gtaataaaca gggttatagc caaccttttc tactaacaaa gtgagttctt
    37861 ggactaattt tgaatcccgg ttgccagaaa aagtagcaga tttcagtggt ttacgattca
    37921 aaaaacgttt agctaaatca tttaaaatag gatcagcaga ttcagaccat tgagtaaagt
    37981 aagtgcccaa gaccccatca tcaagtttga ggtattcttc taaagtaaag cttccgtcaa
    38041 gaaatggcag aagtaattga gaatggagtt caaaactgct ggcatcttct tgatacaact
    38101 cgtgtgcgcg atgaagcaga tgctccaaaa tcacttccat accacgggaa acaggatgga
    38161 agtacacttg cacatacatt tgatatcggc taacgatata atcttccact gcatgcatgc
    38221 cactcatgga aaaagcgatc ccgccttcat aaggacgaat cacacgtaat attcgggtaa
    38281 gatcaaatgt tccatattct gttccagtga aataagcatc ccgaagcaaa tagtccattc
    38341 gatctgcatc gatctgacta gaaatcattt ggacaacttg tgggttgggg taagtttttt
    38401 ggatcacact agctaccttt tcaggaaaac cttcttctac tcggttaaga atctgataca
    38461 cttctgtttc tggagaagtg atgatctcta cagtgatggc ttcatgattc gtatggaaga
    38521 tatgttcaaa tgtatgagag taaggaccat gaccgacatc atgcaacagt gccgcacata
    38581 aagtcaccag ccgttcctga tcatcccagc ctccgttgcc tattttttct tttgaaaaat
    38641 ttcgagaaaa gatatcgcag attcgtcttg aaatctcata aacgcctaat gaatgggtaa
    38701 aacggctatg ttctgctccg tgaaaagtga aagaagaagt gcctaattgt ttaatgcgtc
    38761 gtaaacgttg gacttctttc gaattgatca aatctaagat tactttatgc tggacatgga
    38821 tatagttatg gactggatca cgaaaaactt tttccatagg cagcatttga tatttataag
    38881 gggttgtcat aatgtagctc ctttatgtta ttacgttgtt ccattctctg gatttggttg
    38941 ttccattgct ccagctggat ttcttttttt agccagtcgg tagaatcctg taaatcactt
    39001 gcagaataca gcttttcaaa agcttttgca aatgcttctt tgacttcttt tacaaaaagg
    39061 ggttggtcaa gcagttcttc aagtgttgcc attgtagcag gatccactgc aggatagcca
    39121 tttgtaccaa agtcttcttt taaagcagat tgataaaatc gcttcatgac ttgcccgcgt
    39181 ttttcctgat taccgttgac acttaagtac atcataacag aaatcccatt tttgatccga
    39241 cgctgagcga taccggcaaa ttttttgcca tggatgctta aatcataagt tccaggacaa
    39301 taagaatggg tgatttcgaa tgcttgtatc ggtgcctttg gaaaagcgta tttagtcaat
    39361 aggagcattt gctcgtaagc gtcatctatg gttatttttt ttgtgtcact ttgagggaaa
    39421 atcaaagaaa tatttaaaat tccttcatct gatacgatcc ctaatccacc tgaatttctg
    39481 atcactggag cgtaacctgc atctttcagt gtttggatcc cgtcacttag aaaaggagtt
    39541 cgaacatcct tcatacctaa gatgactgtg cagtcatatt gccagaaatg taaaaagatt
    39601 tgctggtgtt ctttagaata ttccgttaat atatctgtta atgcaaaagg ggaaaagtcg
    39661 tttttttgat aaatgtgctg atctagtcga atcgcagggt tcattcaacc ggctcctttg
    39721 tataaattca tattttctac aatattatac tctaaaatca aagagttgtt ttggaaaaaa
    39781 ggttttctgt aaaaatggac aagttggttg acaaaacaaa agaaactagg aacaatgtag
    39841 ctatgagaag taaaggagaa tgaataatgg aattatcaca aggaacaccg atttcaatca
    39901 aattgcgtac aaaagtaaaa cagcaagggg aagtgcaaga tttttatttt gatttgaaag
    39961 ggcaaatggt gacaattggc gatacgctgt acatacgtta taaagagatt caggaggaga
    40021 cgggcgatga gattcctgta acgatcaaat tagttccaga tggtcatgta cagctgatcc
    40081 gtgcaggaga aatgcggatg cgtttgaaat ttggctataa agaacggtta gaaacatcct
    40141 atcgaacacc atacggtatg atccagattg ctacttttac gaaagaactg catgttagtt
    40201 tgaaagaccg tcccacagca gggaaggttc gaatcgatta tgatctgttc atgggaccgg
    40261 aaaaaatcgg cgaatattat ttgaccttgg attttacagc gtaaatataa aagaaaagtt
    40321 tgattttttg tgtctgattt tgtatgataa ggagtagact gtgaaaggac gtgtcctgat
    40381 tggaaattag tgtatttgaa ggtgcaaaca aaagcgaatt atcaatgatc gaagtagcac
    40441 acgcgatctt agaacaacgt ggcgatgtca tggatttttc tgatttagct aaccagatcc
    40501 aaaactatct tgagaaatca gacagtgaaa ttcgtgattc actcgcacaa ttttatactg
    40561 acttgaatat tgacggcagc tttatctcat taggggataa tcgttggggc ttgcgttcat
    40621 ggtatgctat cgattcgatc gatgaagaag taaaccatgg aatggacgaa gaggatgaag
    40681 atactccacg tcgccgtaaa cgcaagaaag tcaacgcctt tatcaatgat gatgaagacg
    40741 cgatcgatta caatgacgat gacccagaag atacagatct gacagaagaa gatgacgatg
    40801 atctgtttga tgacgatgat gatgaagatg aagaaatcgc tgcttataat tctgacttgc
    40861 aagaaatcgg agcagatgac ccgacagatg atgaagaaga cttgccagga aatatcgaag
    40921 aagatttaag tatcatagac gaagacgatg atgatgaaga ttatgaagag gaagaatttt
    40981 ctgactctga aaaagaataa acattatttt cttacgagag gttgtgacag agacgatcag
    41041 cctatcagaa taaggaggtg tgacacgatt tttgtcacac cttctttttt gtatataaaa
    41101 atcccaagta aatacataaa aaataaaaaa attgaaactt ttagcgcacc ttgtacgtca
    41161 tattaagtga ggcagaaagg agtggagaat gatttttact ggatataaga aaaaacggca
    41221 gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa actgattatc aaaaaatgtt
    41281 ccgtatcacg atgaattacg tacatgataa agaagaggcc ttagatgtga tgcaagatag
    41341 ttttcataaa gccttgaact attttgaaaa agaaaaagag atcgagcatt ttagtgcatg
    41401 gttttatcgg atattgatcc gcacggcatt agatagttgg cgcagacaaa aaaggacgcc
    41461 tgtggactta tttgaaattc aagatatccc ccaacagtta cctttagaaa cttccgttgc
    41521 agagctccat atgattttag ataaaataga cagtccggaa aaagagattc tgatcctcta
    41581 cttttttgaa ggatttcgtt tgaaagaaat tgcaactatc ttggatatga atgaaaacac
    41641 agttaaaacg aaaatgtacc gctcattgaa tgctttacgt caaatcctag aatgaaggag
    41701 agaagagtgc catgtattcc caaaaagaaa atttaaaaaa actaaagaaa acttataaag
    41761 aacaagcaat tcccaaaggg cttaaagagg agatccgcca gcgttttatt atcgaagagc
    41821 aatcatttat aaaagcaagg cggagaaaga gacgtctcca agggttcgga gtatctttgg
    41881 ctatcgtaat gattacaaca agtagccttc tttttaatag tcaagttcga agctttgcag
    41941 aagatttacc catcttagga tcagtcattg aattgatctt aggcgaacgc tttactgatc
    42001 gatctgaaaa aatcgatatt caggtaccga agatcaatac acaaaatgag aaagaaaata
    42061 aaacaattca tggattaaat caaaaatact ttcgcgaagg acaagcagat tttgaaaaag
    42121 ctgaaaaaga atatggaaat tttgaaacgg atcattacca agttttggga gattatcaga
    42181 aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca gatcacccaa acagcagcag
    42241 atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa aaacagtgtc cagctctcac
    42301 ttccactttt attcaaagat gacacttatc tatctgtttt gacaaaagaa gtcaaacggc
    42361 agatggccga acaagtcaag gaagatccat ctaagtacta ttggactgag caagatattc
    42421 aagaagggac gatagaaaaa ccaacacttg ttacgcctac acgaagtttt tatttgaaca
    42481 aagagcatca attagttttg actttttctc aatacgaaat tgctcctggt tatatgggta
    42541 caccagaatt tgtcattcct aagtccgtga caaaaacgat tttggcatcg gaagattatt
    42601 tggatcatta gacgagaagt agttgcgact agcagtttca attttcaagt aagtacaagt
    42661 aaataataag aagagaaaaa gagaaagaag agatatagtg aaaagaaatt atttagtcat
    42721 cagtattatt gctttatccg tgcttttgtt tttcgaaatt acttcggtgg cctccttgat
    42781 caccgaagta aaaggacaaa cacactcttc ggagcattcg tcacaatctt tcaaacaatc
    42841 agaacaatca accattaaaa gccaatcaac tgaaacagct aacgtcaaag aagccattca
    42901 agaatcagcg gaaaaggtac aaagtcaatc atcacaatca acacaagaca gtacagaagc
    42961 aatccaagac ccgactgtca aaagcgtcgc aatcagcttt gatgatggac caggagcgac
    43021 cacaacgccg cagcttctgc gaatcttaaa agaaaaaaac gtgcatgtca cttttttcgt
    43081 cttaggagaa aatacggcgc agcatcctga aatcgtgaag cagacagctg aagcagggca
    43141 cgagattggc aatcacacat acgatcatca gaatctggcc actctttctg cccagtcgat
    43201 gacggaggaa gtgacgaaag cagacactga gatcaaaaaa agtcactggc aaaacccccg
    43261 cttttgttcg tccgccctat ggttctgtca cgaatgtagg agcaacgatc atccagcgac
    43321 cgatcatcga atggtctgtt gattcagaag attggaagac aaggaatcct gatttgattt
    43381 tgcagaagat ccaagcaaca gtatacgatg ggtcaattat tttattccat gatatctatc
    43441 ctgaaacgat tcgtgccgtt cctcaagtga tcgattatct gaaagagcaa ggctatcgca
    43501 tcacgacagt tagcgattta ttagggcatc caacggctgt cgagaactat tatggcagaa
    43561 acgatcatcg gccagttcag taagtgagac aagtatcttt aataaataac acattttaat
    43621 aagtagttga acacataact accttacttg caaaggctta acaatatcta tacagataca
    43681 acaaaaaact gtccaccaat tctctttggc ggacagtttt cacttaaaaa taaagaagca
    43741 actattcgta aattatgcta aaatagcagt agaacgcgtc ctgagggaat cgaacccccg
    43801 tctcaagaac cggaatctta cgtgatatcc actacactaa ggacgcaagc actgggataa
    43861 ttgtaactaa acagataaaa aattacaagg ggattggaca aaaaaatgaa atttgagcaa
    43921 aatttttcac aaaaacagca gcagacacag aaactagcca tgacgcaaca gttgcaacag
    43981 tccatccaag ttttacaata taacagtgag gagctgttgg cattcgttga gaatcaagca
    44041 atggaaaatc cattggtgga agttgttgag ccggaatggc agccagatta cataaaagct
    44101 tcttcatctt cttatgaagg agaagagaca aattacctaa atcagattcc tgatacaaaa
    44161 gggtctttat ttgattcatt gatcgaacaa gtccatttga attaccgaga tacttttttg
    44221 aggaaaatcg ttctttatct tgtagaatac atcgatttga acggattttt gacgattagt
    44281 ttagaagaag ccggaaaaga gattggagct acgcctatcc aaatgctcga tgcgttgact
    44341 ctgattcagc aattagaacc agctggagta ggtgcacgta gtttgcaaga atgtctcatg
    44401 cttcaaacag aacgagatga ttatgcgccg gaattagctt atatcgtgtt ggaagaatgt
    44461 tttgaggaac tggtggagcg aaaatggaaa gaaatcgcac aacgtttttc tgttgatcta
    44521 catgctgttc agcagatatt cgactattta caaacgttga gtccaagccc tggccggatt
    44581 ttcgaccgta gcagcgaatt gtacattatt cctgatgtcc gggtattagt ggacgacgat
    44641 aaaaatgtac aagttatctc aaaccgaaaa aatcagccaa atatacgctt tcaagagagt
    44701 tacttcaaac aaatgtcaca acaagcagat aaagaaactg aaaattattt aaaagaacgg
    44761 aaacaggaat tcgaatggtt gaagaaaacg atcctacaac gtggggatac aattttgcgg
    44821 gttgctcaag tcattgtttc ccggcaaaaa gctttcttta tcgacaagga acgtccaatc
    44881 aagcctctta cgttaaagga agttgcaact gagatagatg tgcatgaatc cacggtcagt
    44941 cgtgcagtca acgggaaata tttggaaaca gacttcggtg tatttgaatt aaaaaaattt
    45001 ttcactacac gaattcctac gaatagtaca gaacagacag aagacctttc agcagataca
    45061 gcgaagaaaa agctgcagga attagtcgat caagaagaca aaaacaagcc gttgtctgac
    45121 cagaaattgg ttgaattgct gaaaaaagat gagatagcga tctcaagacg aacagtagca
    45181 aaatatcgtg atttattagg aatcccgagt tcatcaaaaa gaaaacgtta tgataactaa
    45241 tcagtcaaaa gactagaaaa agtatttcac tttttctagt ctttttagtt gaaaaacgag
    45301 agcaaaactg ttagacttac agtcgtgagg ttgagatttc cctttattga tgtgaaattc
    45361 gaatagttaa tttctttttt tatcgtatat gggtcgtttt aagtctacat tggacgtaaa
    45421 gtgtccaact aaggagatcg ctatgctgga tgaaataaaa atgattgaat ctgttgcacc
    45481 agatattatc gaagtgttgc aagaacgcta taaaatttta cgaaatatct attggatgca
    45541 gccgattggg cgtcgaagtt tatcggaaag tatggggatc acagaacgag tcttacgaac
    45601 agaaacagac cttttgaaaa atctgaattt gatcgacacc tcaaaaagcg gtatgacgct
    45661 gacaactaag ggcgaagaag tttatcaaag ccttgaaaac ttcatggatc agcttttagg
    45721 tacgcatcaa accgaacaac agttggcaga atattttggt aaccaacgct gtatcgttgt
    45781 ttcaggaaac agcgaagagc agaccaaagt ggctgatgca ttcggcgaag cgctgagcga
    45841 agccttagac cgactacttc cagaaggcga gaatatcatc gctgtgatgg gaggaactac
    45901 tatggctgtg gtagccgaac aattgtctaa tctggaaaat aagaaacggc ataacctgtt
    45961 tgtccctgca agaggcggga ttggtgaagc gataactgta caggcaaatt ctgtcagtgc
    46021 aagaatggct gctaaagcaa acggtaacca ccgcgcactt tatgttccgg aacaattaag
    46081 tctggcaact tacaattctt tactcaatga gccatctatc caagaagtct taaacttgat
    46141 tagtgaagcg aattgtgtta ttcatagtat tgggcgtgca ttacacatgg cagcacgtcg
    46201 taagatgact gaaaaagaat tagtcatgct gaaacaagca aatgctgttg cggaatcttt
    46261 tggctacttt ttcaatgaaa aaggtgaagt cgtttataag gtccctagaa tcggcataga
    46321 attgcaagat ttggaaaaag tcccgatcat catggcaatc gcgggcggaa aatcaaaagc
    46381 taaagcaatt cgagcttata tgaaaaatgc accaaaacaa acgtggctca tcactgatga
    46441 agctgctgca aatgagattt taaaaggggt aaccctttaa aataaatatt tttttgattt
    46501 tcataaggag gaaatcttaa atgacagtta aagtaggtat taatggtttt ggacgtattg
    46561 gacgtctagc attccgtcgt atccaagatg tagatggaat cgaagtagta gcaatcaacg
    46621 acttgacaga tgctaaaatg ttggcacact tgttaaaata cgacacaact caaggacgtt
    46681 tcaacggaac agttgaagtt catgaaggtt cattcaacgt aaacggaaaa gaagttaaag
    46741 tattagctaa ccgcaaccca gaagaattac catggggcga actaggcgta gatatcgttc
    46801 tagaatgtac tggtttcttc acttctaaat cagcagctga aaaacattta acagctggtg
    46861 ctaaacgcgt tgttatctct gctcctggcg gaaacgatgt tccaacaatc gtttataaca
    46921 caaaccacga aacattaact ggtaaagaaa cagttatctc aggtgcttca tgtactacta
    46981 actgtttagc tcctatggct aaaacattga acgacaaatt tggtgttgtt gaaggattaa
    47041 tgacaactat ccacgcttac acaggtgacc aaatgactct agacggacct catcctaaag
    47101 gtgacttccg ccgtgcacgc gctgctgctg caaacatcgt tcctaactca acaggtgctg
    47161 ctaaagctat cggtttagta attcccgaat tgaacggtaa attagacgga gctgctcaac
    47221 gtgttcctgt accaacaggt tcattaacag aattagtaac agttcttgaa aaagaagtaa
    47281 ctgttgacga aatcaatgca gcaatgaaag aagcttcaaa cgaatcttac ggatacaaca
    47341 cagacgaaat cgtttcttca gatatcgttg gtatgactta cggttcatta tttgatgcta
    47401 cacaaactaa agtaatgaca gttggcgaca aacaattagt taaaactgtt gcttggtacg
    47461 acaacgaaat gtcatacact gcacaattag tacgtacttt agaatacttc gctaacttat
    47521 aagatagaca aacgtacaag tgtaaattat agacatgtac atttaaaagc ggggaagcaa
    47581 tcgcgcttct tccgcttttt ttgataataa gtttattcag gaggaaataa aatggctaag
    47641 aaaacagtga aagatatcga tttaaaagac aaaaaagttc ttgtccgtgt tgacttcaac
    47701 gttcctttga aagacggcgt gatcactgac gacactcgta tcaaagcagc attgccaaca
    47761 atcaactacg ttctagaaca aggcggaaaa gcaatccttt tctctcacct tggacgtgta
    47821 aaaacagaag aagataaaga aggaaaatct ttagctccag ttgcaaaacg tctaggcgaa
    47881 ttattaggta aagaagtaac attcgtacct gaaactcgcg gagaacaatt agaagaagca
    47941 atccgcaaca tgaatgacgg cgacgttgtc gtattcgaaa atacacgatt tgaagatatt
    48001 gacggtaaaa aagaaagcaa aaatgataca gaactaggta aatactgggc ttcattaggc
    48061 gatgtatttg ttaacgatgc tttcggtaca gctcaccgtg cacatgcttc taacgtaggt
    48121 atcgcttcaa ctggtatccc aacagttgct ggattcttga tggaaaaaga aatcaagttc
    48181 atcggtgaag cagtagaaga accaaaacga ccaatgattg caatccttgg tggcgcaaaa
    48241 gtttctgata aaatcggcgt aatcgaaaac ttgattccta aagcagataa aatcttgatc
    48301 ggtggcggaa tgacttacac attctacgaa gcaaaaggaa tcaagatcgg taactctcta
    48361 gtagaagctg ataaagtgga actagcaaaa gaattaatcg aaaaagcagg cgacaaactt
    48421 gtcttgccaa tcgacaatgt ctgtgcacca gaattttcaa atgatgttga aacacaagta
    48481 atcgaaggag acattcctga tggtttgatg gctttagaca tcggcccagc atcagtaaaa
    48541 ttatttgctg atacattaca aggtgcaaaa acagttgttt ggaacggacc tatgggtgta
    48601 tttgaaatga gtaactttgc taaaggtaca atcggtgttt gtgaagcaat cgctaactta
    48661 gaaggcgcaa caacaatcat cggtggcgga gactcagccg cagcagcaga acaattagga
    48721 tttgctgaca aattcacaca catctcaaca ggtggcggcg caagcttgga attgcttgaa
    48781 ggtaaagaat taccaggact agcagcaatc aacgataaat aaacgaccaa gtttgctcaa
    48841 ttctgacgag taatcgaaca aaaggtgaag ggagttttca tgactcccta gccatttttc
    48901 aataaattaa ggagtgtgtt cctcaatgcg taaacctatt atcgcaggta actggaaaat
    48961 gaacaaaact gcatcagaag caaaagaatt tgctgaagca gtaaaaaaca aaatcccttc
    49021 aaatgacaaa gttgattcag tcatcggatc tcctgcatta ttcttacaag aattaatgac
    49081 atcagctgaa ggtacagaac taaaaatctc agcacaaaac tgctactggg aaaactcagg
    49141 tgcgttcact ggtgaaactt caccagcagc tcttgctgat ctaggtgttc attatgtgat
    49201 catcggacat tcagaacgtc gtgaatattt ccatgaaaca gacgaagaca tcaataaaaa
    49261 agcgaaagct atctttgcaa atggtatgac accaatccta tgctgcggcg aatctttaga
    49321 aacctatgaa gcaggcaaaa ctgctgaatg gatcgaagga caaattacag ctggtttagc
    49381 tgatctttca gctgaacagg tgtcaaacat ggttattgct tatgaaccaa tctgggcaat
    49441 cggtactggt aaatcagctg acgcacaaat cgctgacgaa atttgtggag ttgtacgtca
    49501 aacagttgaa aaactttatg gaaaagaagt atcagaagca gttcgtatcc aatacggcgg
    49561 ttctgtaaaa ccagaaaata ttgctgaata catggcaaaa gaaaatgttg acggtgcttt
    49621 agtcggtggc gcaagcttgc aagcagattc attcttgtca ttgttagacg ctgtaaaata
    49681 attgttgaaa atttgcgaaa aatgcaagaa atgtttgcac taatgagcaa aattaactaa
    49741 aatcgtatat gagggaaatc ccttaggaaa aaacaaactc aaaggagaga caaaacatgt
    49801 caattattac tgatgtttac gctcgcgaga tcttagactc acgtggtaac ccaacaatcg
    49861 aagtagaagt atacacagaa agcggagctt ttggccgcgg aatggttcct tcaggtgctt
    49921 caactggtga atacgaagca gttgaattac gtgacggaga caaagctcgt tatggcggta
    49981 aaggtgtaac taaagctgtc gacaacgtaa acaacatcat tgctgaagca atcattggct
    50041 acgatgttcg tgaccaaatg gctatcgaca aagctatgat cgctttagac ggaactccta
    50101 acaaaggtaa attaggtgcg aacgctattt taggtgtttc tatcgctgtt gcacgtgcag
    50161 ctgctgacta cctagaagta cctttatacc actacctagg cggattcaac acaaaagtat
    50221 tgccaacacc aatgatgaac atcatcaacg gcggatcaca tgctgataac tcaatcgact
    50281 tccaagaatt catgatcatg cctgttggcg ctccaacatt caaagaagct ctacgtatgg
    50341 gtgctgaagt attccacgca ttagcttcaa tcttgaaagc tcgcggatta gcaacttctg
    50401 ttggtgacga aggtggattt gctccaaacc ttggttcaaa cgaagaaggt tttgaagtaa
    50461 tcatcgaagc aatcgaaaaa gctggctatg tacctggtaa agacgttgtt cttgctatgg
    50521 atgctgcttc ttcagaattc tacgacaaag aaaaaggtgt ttacgtacta gctgactcag
    50581 gcgaaggcga aaaaacaact gacgaaatga tcaaattcta cgaagaatta gtttctaaat
    50641 atccaatcat ctcaatcgaa gatggattag acgaaaacga ctgggacgga ttcaagaaat
    50701 taactgacgt attaggcgac aaagttcaat tagttggtga cgacttgttc gtaacaaaca
    50761 ctcaaaaatt atctgaaggt atcgaaaaag gaattgctaa ctcaatcctt atcaaagtga
    50821 accaaatcgg tacattaaca gaaactttcg aagctatcga aatggctaaa gaagctggct
    50881 acacagcagt tgtatctcac cgttctggtg aaacagaaga ttcaacaatc tctgatatcg
    50941 ctgttgcaac aaacgctggc caaatcaaaa ctggttctct atcacgtact gaccgtattg
    51001 ctaaatacaa ccaattacta cgtatcgaag accaacttgg tgaagttgca gaatacaaag
    51061 gtttgaaatc tttctacaac ttaaaaaaca aataattctt ttctaatgaa gatacatgat
    51121 cgactattga aagatagtcg agtatgatca cttagttaag taatcgaact ctctacttgc
    51181 tgaaatatca gcaggtagag agtttttttg caactagctt ctgtatatga tgtttacaaa
    51241 gaatggtaca agaaaagatc atccttgaaa aaggaaagct aacaataact agcatcatat
    51301 aagagctttt aaggatagaa cagtagaata ggaatctatg ggcaagtcct ctcgtataaa
    51361 ta
//
LOCUS       DAZKCY010000006        51070 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000006 DAZKCY010000000
VERSION     DAZKCY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.006"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            182..1027
                     /locus_tag="ACG8XC_000316"
     CDS             182..1027
                     /locus_tag="ACG8XC_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9190043.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1308..2528
                     /locus_tag="ACG8XC_000317"
     CDS             1308..2528
                     /locus_tag="ACG8XC_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP9190044.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2525..5245
                     /locus_tag="ACG8XC_000318"
     CDS             2525..5245
                     /locus_tag="ACG8XC_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9190045.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFIFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5229..6173
                     /locus_tag="ACG8XC_000319"
     CDS             5229..6173
                     /locus_tag="ACG8XC_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9190046.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6242..7252)
                     /locus_tag="ACG8XC_000320"
     CDS             complement(6242..7252)
                     /locus_tag="ACG8XC_000320"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9190047.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7432..7776)
                     /locus_tag="ACG8XC_000321"
     CDS             complement(7432..7776)
                     /locus_tag="ACG8XC_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP9190048.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7769..8203)
                     /locus_tag="ACG8XC_000322"
     CDS             complement(7769..8203)
                     /locus_tag="ACG8XC_000322"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFP9190049.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8341..9078
                     /locus_tag="ACG8XC_000323"
     CDS             8341..9078
                     /locus_tag="ACG8XC_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190050.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9071..10282
                     /locus_tag="ACG8XC_000324"
     CDS             9071..10282
                     /locus_tag="ACG8XC_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9190051.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10486..11268
                     /locus_tag="ACG8XC_000325"
     CDS             10486..11268
                     /locus_tag="ACG8XC_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFP9190052.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11338..12000
                     /gene="trmB"
                     /locus_tag="ACG8XC_000326"
     CDS             11338..12000
                     /gene="trmB"
                     /locus_tag="ACG8XC_000326"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP9190053.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12098..13852)
                     /gene="ade"
                     /locus_tag="ACG8XC_000327"
     CDS             complement(12098..13852)
                     /gene="ade"
                     /locus_tag="ACG8XC_000327"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP9190054.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13984..14913
                     /gene="comGA"
                     /locus_tag="ACG8XC_000328"
     CDS             13984..14913
                     /gene="comGA"
                     /locus_tag="ACG8XC_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFP9190055.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14870..15919
                     /gene="comGB"
                     /locus_tag="ACG8XC_000329"
     CDS             14870..15919
                     /gene="comGB"
                     /locus_tag="ACG8XC_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFP9190056.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15916..16194
                     /gene="comGC"
                     /locus_tag="ACG8XC_000330"
     CDS             15916..16194
                     /gene="comGC"
                     /locus_tag="ACG8XC_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFP9190057.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16169..16639
                     /gene="comGD"
                     /locus_tag="ACG8XC_000331"
     CDS             16169..16639
                     /gene="comGD"
                     /locus_tag="ACG8XC_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFP9190058.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16611..16958
                     /locus_tag="ACG8XC_000332"
     CDS             16611..16958
                     /locus_tag="ACG8XC_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190059.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            16921..17346
                     /gene="comGF"
                     /locus_tag="ACG8XC_000333"
     CDS             16921..17346
                     /gene="comGF"
                     /locus_tag="ACG8XC_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFP9190060.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17318..17677
                     /gene="comGG"
                     /locus_tag="ACG8XC_000334"
     CDS             17318..17677
                     /gene="comGG"
                     /locus_tag="ACG8XC_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFP9190061.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17849..18856
                     /locus_tag="ACG8XC_000335"
     CDS             17849..18856
                     /locus_tag="ACG8XC_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9190062.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18881..20065
                     /locus_tag="ACG8XC_000336"
     CDS             18881..20065
                     /locus_tag="ACG8XC_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFP9190063.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20502..22085)
                     /gene="abc-f"
                     /locus_tag="ACG8XC_000337"
     CDS             complement(20502..22085)
                     /gene="abc-f"
                     /locus_tag="ACG8XC_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFP9190064.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22190..22318)
                     /locus_tag="ACG8XC_000338"
     CDS             complement(22190..22318)
                     /locus_tag="ACG8XC_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190065.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22663..22806)
                     /locus_tag="ACG8XC_000339"
     CDS             complement(22663..22806)
                     /locus_tag="ACG8XC_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP9190066.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23029..23508)
                     /locus_tag="ACG8XC_000340"
     CDS             complement(23029..23508)
                     /locus_tag="ACG8XC_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9190067.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23581..23865)
                     /locus_tag="ACG8XC_000341"
     CDS             complement(23581..23865)
                     /locus_tag="ACG8XC_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190068.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23943..24137)
                     /gene="ssrS"
                     /locus_tag="ACG8XC_000342"
     ncRNA           complement(23943..24137)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8XC_000342"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24209..25495)
                     /locus_tag="ACG8XC_000343"
     CDS             complement(24209..25495)
                     /locus_tag="ACG8XC_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFP9190069.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25633..26304
                     /locus_tag="ACG8XC_000344"
     CDS             25633..26304
                     /locus_tag="ACG8XC_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFP9190070.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26270..26758
                     /locus_tag="ACG8XC_000345"
     CDS             26270..26758
                     /locus_tag="ACG8XC_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFP9190071.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26771..27718
                     /gene="prmA"
                     /locus_tag="ACG8XC_000346"
     CDS             26771..27718
                     /gene="prmA"
                     /locus_tag="ACG8XC_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP9190072.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27719..28483
                     /locus_tag="ACG8XC_000347"
     CDS             27719..28483
                     /locus_tag="ACG8XC_000347"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP9190073.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28716..30929
                     /locus_tag="ACG8XC_000348"
     CDS             28716..30929
                     /locus_tag="ACG8XC_000348"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP9190074.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHLKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30940..31386
                     /gene="dtd"
                     /locus_tag="ACG8XC_000349"
     CDS             30940..31386
                     /gene="dtd"
                     /locus_tag="ACG8XC_000349"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP9190075.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31557..32816
                     /locus_tag="ACG8XC_000350"
     CDS             31557..32816
                     /locus_tag="ACG8XC_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFP9190076.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32905..33852)
                     /locus_tag="ACG8XC_000351"
     CDS             complement(32905..33852)
                     /locus_tag="ACG8XC_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9190077.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34246..35538
                     /gene="hisS"
                     /locus_tag="ACG8XC_000352"
     CDS             34246..35538
                     /gene="hisS"
                     /locus_tag="ACG8XC_000352"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP9190078.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35553..37322
                     /gene="aspS"
                     /locus_tag="ACG8XC_000353"
     CDS             35553..37322
                     /gene="aspS"
                     /locus_tag="ACG8XC_000353"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP9190079.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37431..38306
                     /locus_tag="ACG8XC_000354"
     CDS             37431..38306
                     /locus_tag="ACG8XC_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9190080.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38572..40455
                     /locus_tag="ACG8XC_000355"
     CDS             38572..40455
                     /locus_tag="ACG8XC_000355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9190081.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40530..40814)
                     /locus_tag="ACG8XC_000356"
     CDS             complement(40530..40814)
                     /locus_tag="ACG8XC_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9190082.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40804..41538)
                     /locus_tag="ACG8XC_000357"
     CDS             complement(40804..41538)
                     /locus_tag="ACG8XC_000357"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFP9190083.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41667..42290
                     /locus_tag="ACG8XC_000358"
     CDS             41667..42290
                     /locus_tag="ACG8XC_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP9190084.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42482..43603
                     /locus_tag="ACG8XC_000359"
     CDS             42482..43603
                     /locus_tag="ACG8XC_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP9190085.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43610..46738
                     /locus_tag="ACG8XC_000360"
     CDS             43610..46738
                     /locus_tag="ACG8XC_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9190086.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46757..49951
                     /locus_tag="ACG8XC_000361"
     CDS             46757..49951
                     /locus_tag="ACG8XC_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFP9190087.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50097..50804)
                     /locus_tag="ACG8XC_000362"
     CDS             complement(50097..50804)
                     /locus_tag="ACG8XC_000362"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9190088.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16705 a         8936 c        10611 g        14818 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctcatataaa
       61 tgtcctttcc tattcttaaa atgatgtttg tacttactta aattaggtat aattagtata
      121 tgttttgtga gaaatttaga cttacaataa gcagtgaatt tataatcaac gtataggaga
      181 catggatgaa atgaaactaa ttggtataga tttagacggt actcttttga attctgacca
      241 gagcattagt caaaaaaatg caagaacaat gaaacattta ccggaagact gttttccatt
      301 catttgttct ggaagagaag tagaagacat taataatatt ctcaaaaaat ctggtttaag
      361 gataccagca gtagggttaa atggttctgt tggatttgat ggagaaaaaa agatatttga
      421 gtcttccttc gataaaaatg atgtaaaaaa aattgattct gtcctatcga aatttccaac
      481 caaaatatat acaaatctcg gaagttatga atctcagaat tataaaaata aaatgaaaaa
      541 aatttttcac gaggtaggga gtgaattttc tatcaaagag ctaaactatg agcttgagta
      601 tgaaaaatct atttgttcaa tcccttataa taatattgag gaaattatag aaaaggatgc
      661 tatcagcatt tacaaatttt ttatttttat tcctaatcgt caattgaaag ctaatatatt
      721 taaccatttg aaaaagatgc taaatgtgtc tgttactgaa tcggcacctg taaatattga
      781 aatcgtacct caaaatgtat ctaaaggtgt ggtgtttgat cgtttggaat caatctacaa
      841 tttaaagaaa cctttgagaa tcgcgatcgg agatagttta aatgacttat ctatgtttga
      901 aagtgcagat attagttttg caatgggaaa tagtcatcaa gaaatcatga aaaaagctga
      961 ttatgtcact gtttcaaatg ataaagacgg agtatctcaa gcatttgaaa gaattcttac
     1021 catataaact aattaaggta gtatagaaca tagaattcta aatacggaaa aatggagcat
     1081 aaattccagt acaaatagta cttcttcagt gtcaaacgaa gcgaatataa catagatatt
     1141 taggattgtg acaaaaatca tgtcacaatc tttttttcga ataaacttat tttttgaagt
     1201 cggccattta tcgtaatttc tttgcataat acgaacaaat tttcggttat tgatgaaaaa
     1261 aaacattgaa aatggtatga taaaagaaaa aaaggaggaa atttttaatg gtgaaattta
     1321 tccatgcggc agatttacat atggatcggt catttgaagg actgacaact cttgataaaa
     1381 ctgtacagga aaaactattg aagacgaatc tgaccgtttt atcaaatatc attgaacagg
     1441 caataataaa taacgttgat tttgttttac ttgcaggaga taattttcat caaaatcgtc
     1501 cttcattaaa aatacagaaa catttttcag aacagatgaa acggttagaa aagaaccata
     1561 tacctgtatt tataatattc ggaaatcatg atttctatca aaaagagcgg tattggttct
     1621 cctttcctaa aaatgtccat ttatttacat ccgaaacggt agagacaaaa aaaataacga
     1681 taaaaagtgg agaaactgtc tctctcagtg gatttagtta tcgtcaaccg tggattcaaa
     1741 aagacaaagt gatggaattt ccttctagag aactcacaga ttaccatatt gggttgtacc
     1801 acggggaacc tggcgtctct caaaaaggaa actatgctcc tttccaaccc tcaaagatgc
     1861 aagaaaaggg atacgattat tgggctttag ggcatatcca tgtgccaacg gtcttaaatg
     1921 aaaagcaaac aattgtttat ccaggtgcgc ctcaaggtca tactaaaaag gaacaagctt
     1981 ctacatctat tctactagtt gaattaagta aaggaagttg ccaaatacat ccgattaaag
     2041 tagcagaggt ctattggaag acaaaggaaa tatcattgag gaaagcaaga acgacaaaag
     2101 aggtcattgt tcacattcgt cagcagcttt caaaagtaga ggatggattt agtttaattg
     2161 agataaaatt aaaagattat gaccatctca atacagatgt tttggaacgg atacaatcag
     2221 gagagctatt agactatttg ttagaggaat tctcagatag gataaatgat ttctttattt
     2281 ggcgtatttc tctcatagag aatacttttt atacgaagac cccacttgct gcttctgcta
     2341 aattgatgga acagttattt caatactatc agacacctca agatttccaa caaattttaa
     2401 atgaagtata tagtcatcaa gaagcggcta gaatattgag cgaactgccg gaatatcaag
     2461 aagaaacact tgaaaaagcc aagcagctgc ttaatcaaga ttttttattt gaggaggatc
     2521 aggaatgaag attttaagga tcgagattgc tgcttttgga aaatggcgtc aaaaaagttt
     2581 tgacttttat tcaggcaatc agctgatcta tgggggaaac gaagcaggaa aatcaacgat
     2641 ttatcaattt attcaagcaa tattatttgg tttcccttca aaaggccgta aaaaaaagga
     2701 ttatacacca aaagacggct cagcctatgg cggcaaaata tggcttaaac atcctgtata
     2761 tggtgaattt gctgtcgagc gctacaagca gcaaaatcgc ggaaaatcga aggtgtggct
     2821 gggggatcaa gtgggaagtg atgagttact agaaaaactg atcgcacctt tgacgaaaga
     2881 gttattccag caggtattta ttttccagca agagcagttg atggaactag atcatttgag
     2941 agaaaaagag ctacatgatg cgctgatttc tttaggcatc acgggcagtt ctcaagtatt
     3001 ccaaaagcgg caagaatatt atcagaaagc acaacaatta ttcaagaaaa aaggccaaaa
     3061 attactaatc aatcaaaaat taaatgaatg gaaagagctg caagaaaaaa ttcatcaaaa
     3121 acaagaacag gaagcttatt ttggcaagtt gatccaacaa gaacaggaat acgagtcgca
     3181 acagcagcag ttattggcac aattaaaaga ggcgaacaaa caattttcat tagccagaca
     3241 gcaagaactt aatttttctt tatatgaaga atggcaatct ctcaaaaaaa acagcactac
     3301 aggtcttcct gatgagcaac ttcttttaga tctggagtct ttctccaaac ggtatcagca
     3361 aacaaatgag cgtttggaag aattagaagc aacactggaa aagcatagcg ggatggatca
     3421 gcagtcagcc agatattatt tttatttaga gcaggagcag gaactaaaag atctccagca
     3481 acttcaattt acagcggaac aattaaacga agaatggcaa agattacaaa ttgagactag
     3541 agaaacaaat tcagaattga agattttgga acaaaggtgg cattggcaga aaaatccgtt
     3601 accggaagaa tttttatatg aagaagaatg gaagcatctt ctggctgaag aaagaaggat
     3661 tgctgatcta gaaagccaga tttctgtgcg tctccaaata gctaaagaac agcaagagat
     3721 gattgaagca gaagtgaatg acttggaaga gacctatcca gcattattac aaaatcagtc
     3781 agagatagca ccagtctctt catctaaacc gtatatgctg atggctggcg gagcaataat
     3841 cattgcaggt atatttgtac caatgccctt gaaagtgatc cttttactaa ttggtgcagg
     3901 catgattggc tatttcttct acaaaaggca accaaaaatt aaaactaaaa atgatttctc
     3961 tgagataaaa ggaatgtggc aagaaaaact aggacaactt gatctagcct tagctcaatt
     4021 agctgaagca gaagaaaaac aaaagagaac tggagaagaa aaacagattc ttcagcaaga
     4081 agtatatcgt tttgcggatc atcggcactt aggaaagatg gatacgctca aactcatgaa
     4141 agaatatgga caggaagtca tagattatca gaaattatat ggcgtatttg agcggaaaca
     4201 taaaagagaa caggaaatac aagaaaaact acgacaaatc gaccaaaaat ttgagtttgt
     4261 taaagaatgg ttaccgatac aagataagac aatttcagaa aaaatggtat tgctttcccg
     4321 ttttatacaa gagatggaac agatgaaatt tgccagaagc tatcagcaaa atactttatt
     4381 gaagcaagaa atcaatcaat tgaaaaagaa acaaaaagta tggatacaag aatacagcga
     4441 acaattgact caagcaggct tatcttatcc ttcggaagta cctgtctttt tacaaaaagc
     4501 caaacaagcc gaacagcaga tgaaaagata caatgaatta tcggaaatgc ttactccact
     4561 atttcctgaa gagctgacaa aagagaaatt atctgtaaga ctgcgccaac tgaaagaaaa
     4621 gcaatcccaa ctccagcaag aagaaaaaga agcaagtgaa aatcggcaac gactccaact
     4681 tcaagtagaa gagttgcaaa gagacggcac attggacgaa ctttatcaac aagctagccg
     4741 gcaaaaagca aaattacagg aattactgga ggaatgggga gcacttgaaa tcgccgggac
     4801 attcttaggt gatctttcaa cagagatgag tgaaagacaa ttaccccagt tgttaagagc
     4861 agcaggtgat tatttaggta ttttaacaaa aggaaattat cagaagatat tactgaaaaa
     4921 tgatacactg actttaacag acggatatac agaatttccc atctatgagt tatctacagg
     4981 tacgaaagat cagctgatta tggcgatgag atttgcttac ctctcattag aagctaaccg
     5041 ttcgatatgc ccgatcatca ttgatgatgg ctggcttcat tatgatcatc agcgaaagta
     5101 tcaattagct gaacttttag ctaaatttgg agaaaagtat caaataattt gtttttcttc
     5161 tgataaagaa atggtaagct attaccatga gatgaaccaa atcgtaaaac aattagaagg
     5221 agcaaataat gaaaaaaatt cgtgatttag taatcgatga gcagtttgaa caatttgtcc
     5281 tgatcaaaag tgcagatgta agaatcgcta aaaacggaaa aaaatttatt gcttttactt
     5341 tccaagatac ttcaggtact atcgatggta aatactggga tgcttcggag gaggaaatca
     5401 ataaatttac agccggcaca gtcgtttttc taaacggtaa aagagaggtt tatcaaggaa
     5461 accctcaagt aaagattctt catatgcgct taacaagggt agatgagcca ggagagccta
     5521 gtttatacat ggaaagagcc cctttgaaga aagaagaaat ggaagaagaa atcaatcaat
     5581 tgttgtttga gatcaccaat gcgcattgga atcgtattgt tcgctatcta cttaataaat
     5641 atcaaaaaca attttttgag tttccggcag caaaaagaaa ccatcatgca tttgccagtg
     5701 gattagctta tcatactttg acaatgcttc acttagggaa atcaatcgcc gatgaatatc
     5761 ctgagctgaa tcgagcattg ttgtatgctg gtatcatttt gcatgattta ggaaaggtga
     5821 ttgaactttc cggtcctatg tcgacagaat atacgcttgc aggaaatttg atcggtcatc
     5881 tggtattgat tgacgaagaa ataactaaag cctgtcttgc gttgaaaatc agcgaagctg
     5941 acgaagatgt tctcgtatta cgtcatatgg ttttagcaca ccatggctta ttagaatatg
     6001 gctcacctgt acgtccaaga ataatggaag cagaaatatt acatcaaatc gacaatatcg
     6061 atgcttcgat gcagatgatg ttgacttcga ttcgtcaaac agaaccagga aaatacaccg
     6121 atcggatttt cggtatggac aaccgaagtt tctatgtacc aaaagattta tagcaaaaat
     6181 ttattttttt cattcaaaac aaaaaaagcg agacataagt cttcgctttt tttgttttct
     6241 tttattttga tgaatcagtt gtttcttcag ttgaagattc ttttgttgaa tcagttgatt
     6301 ttgtgtcaga actctttgtt gaagctgtag tttcacttga gtctttcgta cttgatgaag
     6361 ttgttgtgaa tgcactcaac acattttcaa aggcatcatc tttgattttt acatttgcat
     6421 cttttaattc ttcaccgatt actttagttg tgaatgcatt gtcacttagt ttagtttcag
     6481 tagcgatatc ttttagttga tctttgtatt tatccatgtc attgccttta ttttggtttt
     6541 tcaccatttt gacaacgtag tattctgtcg cataagaagt aggatttgtt gtagtaataa
     6601 catcggagat ctcaccgtct ttcaatttga aagctgcttc ttttacttct gcaggaatag
     6661 ttgttgtcgt tgagtcgaat ttaactttac cgccatcttc ttttgtctct gtatcagttg
     6721 atttgtcttt ggctaattta gagaaatcat ctccatcatc cgctgatttt ttcacatctt
     6781 ttgcttcatc ttcacttgat aatttgatga tttgtgcttc tacttctgga tggaaagatt
     6841 tccaagcagt tttcaaatcg tcatctgtaa tatctacgtg agctttcaag ccagcttcaa
     6901 aagctaaatt gttacgaata tattctttat aagtatcttt cgtatatcca gcagcttcta
     6961 attgtgattc aaaagtatca cctaatgatt tcgcttgttt gtcgtattct gcatccactt
     7021 gcttatcggt tactttatcg ccatatttgt tattgaatac tttataaatg atcatccgtt
     7081 gtactaagga ttggttcgat gattcaagtt ttgcttcatc atagaaatca gatacagtga
     7141 tcgttccgcc tttcatcgtt gcgatatctt tattagtatc gcctgaacaa gctgctagag
     7201 ttaaaacagc caacgcacta gtagctgcca aaataattga tttttttttc attaaaattt
     7261 gcactcctgt aattagttat tatttttata tattaactat accatatccc atttccttta
     7321 aaaacgttgt cgtggtagtt tcaaacaaaa ttcaaaattt catcacattc ctaggaaata
     7381 cgcaaacttt tctcacttat atagttaaat ctgttgagaa aataatcatt attatatttg
     7441 ttcaggatac tctggcagat cagcttttag tttttctact tgttcattga tttgtgtgat
     7501 acgtggttcg gcttggaatt taaaatcttc gatatctttt ttgattgctt cttggaaagc
     7561 aggaatcaca gattcagctg tttcttttgt ttcgatcaat gcgcttttaa aatgattcag
     7621 gctgttattt aaatcatttg tcagagttgt tgcttcttcc aattcttcga tcagtttctt
     7681 tcttgtttca ttcccgctcc tgggcgccaa taacagacct ccgacagtgc cagcagcagc
     7741 tccaaataac agtcctttga aaaagttttt aatcatcgca ggacacctgc tttacgattg
     7801 tttcttgaat agcttttagt tcttccgatc cataactatc ttgatgattg acaaagtgaa
     7861 tagaaaaatc atcctctttg ctgtagcgag ggactaaatg gatatgtgaa tgaaaaacag
     7921 attgataggc tagttcacgg ttgttattca agatattaag acctttcatt tctggaaaag
     7981 ctttttccaa tgcttgagca atcttaggca cacgcgaaaa aacctctcct gcaagttcgg
     8041 gatcatattc aaaaatatct gctacatgac gtttaggcac aactaatgtg tgtcctttcg
     8101 ttgcttgaga gatgtctaaa aaagcataga ccttgtcgtc ttcatatatt ttatagcttg
     8161 ggatttcttg attgatgatt ttgcaaaaaa tacagttttc catataaaac atcctttcta
     8221 taaagaataa atacttcagc ttaactttac cataagttaa agctattgta aggaaaaaaa
     8281 caaaataccg tcattttctt cgtggtataa taaataaaaa taaatggcgg aggaaaaaga
     8341 atgagtttaa ttatcgatca tgtcacaggg gggtacggac atctccctgt gttaaaagat
     8401 atcagtttcg atgtacagcc tggagagata gtagggttga ttggactgaa cggagcaggg
     8461 aaaagtacga ccatcaagca cattatcggt ctgctggaag cttcaaaagg aacgatagaa
     8521 atcaatcatc tatccttaaa gaaaaatacg gaagaatatc gcaaacaaat tgggtatatt
     8581 ccagagactc cttctttata tgaagaattg acattgagag aacatatcga agtcactgcg
     8641 atggcctacg atatacctat ggatatagca atgaaaagag cagaaaagtt gttggagact
     8701 tttcggttat ctaatcgtct tgactggttc ccagctaatt tttcaaaggg aatgaaacag
     8761 aaagtgatga tcttatgcgc ctttttgatc gagccttctt tatatatcat cgacgaacca
     8821 tttttaggct tggatccact agctatcaat gcattgcttg aattgatggt agaaatgaaa
     8881 aaagaaggag ccggtatttt gatgtctacg catattttag ctactgcaga aaaatattgt
     8941 gatcgttttg tggtcttaca taacggagag atacgagcag acggttctct tgagatgttg
     9001 agaaaagaat ttcagctgcc ggattcttct ttggacgaaa tctatattgc tttaacaaag
     9061 gagcaggcac atgagtagtt tttttcaaaa acgtttagca agacatcaaa agaaaatgat
     9121 gaaatatatg cgttatgtat tgaacgatca ttttgtttta gtttgtcttt tcttgattgg
     9181 cggtgtggga ttatattatt ctaattggtt gaaaaccctg cagccaccgt ttgcaattgg
     9241 agggctcata atctctgtat tttggatgct ctgtttattc gtgggtaaga tggctacttt
     9301 tgcagaggca gcagatattg tttttttact tcctaaagaa aaagaaatgc aaacctattt
     9361 ggaacgagct tttcgctatt cttgcatttt tccgttgatc tttttgatat tggcatgcgg
     9421 attggccatg ccattagtgg tggttagtac ggagcaatcc ttttcgatgt ttttcgtcta
     9481 tctgatcata ttatggagtt tgaaattcag tcatctgcaa atcaagagag cagaattgtt
     9541 tcgactagat caacaagtta ttcgttattg gaaattaggc tggttttttt cttctctgat
     9601 ctttttgttt ctatcatttt attggctaat atgggcagga atagcaggtg cattagtaca
     9661 ggcaagtcta tattactggt ttttgtggaa gcaacaagaa aaaggattgg attgggagaa
     9721 aatggtgcat gtagaagaac ataggttgca tcagatctat caatttatca atttattcac
     9781 agatgttcct gaaattacgg caaagatcaa aagaagacgc tatttggatc ctatattgac
     9841 tcatattaaa agagaatcaa aaaacactta tctttacttg tttgcaagaa gattggcaag
     9901 aggttctgat tttagtggtc tttatttccg attagtaatt ataggtgggt tattgattgc
     9961 cggagtagtc gacttttatt ttagtctgat tatcggtttc ttatttttat atttgatcgg
    10021 tttccaactg ttacctttag cgaaccagtt ccgctatatg gttctcgttc agctttatcc
    10081 aatccataag aatcagaaga agcaggcgat tcagtatcta ctcacatggg tattaattat
    10141 cgcgtcggtt atttttggtt tgattgctgc agtcgtatta gatgggcagc agcgatgggt
    10201 tccgctagcg gcttatttga ttgttactat gctctttgtt aaactatatg taccaactcg
    10261 tttgaagaag atgtcagact gaaaacaaag ctgtgtaggg taaaaatgag aatgaaaaat
    10321 agagcgttga cagtaagaat gctgttcgtt ctattttttt tgagaaaagc tggttttttg
    10381 aatggcaatt aaatgaaaaa gaacgtatat gtgcttgacg tgtagtgttc aggcgactag
    10441 aataataagt actgcaaaac aaagagtaag aggacgggag aatgcatgat ggagtttcag
    10501 ttagaccaag agtggcggtt acgtcccatc aaaggcgaca caggaaaaac atacataggc
    10561 atgcgtgatg aggacaaagt ctttattaaa cgaaacacga ctccgatgtt ggctgctctt
    10621 tcaagggaag gaattacacc aaaacttgtt tggacaaaac gaacggggaa tggcgatact
    10681 ttgacagcac aagagtggct ggaaggtcgc ttgctgactc ctaatgagat tggtcaaaga
    10741 aacgacgtga ttgatgtttt gtaccattta catcattcaa attctcttaa aaccatgctg
    10801 acccgtatcg gtggtcgtgt catgacgccg gagaagatgc tgcaaaaata tgaaaaagaa
    10861 ttaccggaag aactccagaa aaatcaattt ctgcaaattg tccatcgttt tcttcgtgag
    10921 cagatcccgt caatagataa ggaaaactta tcagtagtac acggagatgt aaaccatcgt
    10981 aattggttag tatgtaaaaa ttatttatat ctagtcgatt gggattcaat catgtttgca
    11041 gaccctgcgg tggatattgc aacgatttta ggaaattatg tgccactgtc tagctggaat
    11101 cagtggctga tcagttatgg catacgcccg actgacgaaa tgcttgaaaa atttcattgg
    11161 tatgcggcca tgaatttgct ccaagaaatc accagatatt gcaaacgtga agatcatcga
    11221 cggatgaacg aagaaatttt acaattaaaa cgaattttca gtggctgagg tttggcccaa
    11281 actctgcgga gatttggcca gaccttttta attagaggaa aggaatggag ataaataatg
    11341 cgtgtccgaa atcgtccggg agcagcggaa atgttagcgg ctcacccgaa ttttgtaatt
    11401 agtgatccaa cattgtggaa gggaaaatgg aatgaattgt ttgaaaacga tcatccaatc
    11461 cacattgaga ttggtatggg gaaaggccag ttcatcactg gaatggcaaa agcccatcct
    11521 gaaatcaatt atattggagt agaaatgcag gtcagtgttg tttcgattgc actagacaag
    11581 ttgatcgaac aaccgcttcc taacctgaaa cttctacatg tggatggatc agcgttgaca
    11641 gagtatttcg ctgacagcga agtagatcaa atctatctga atttttccga tccatggcca
    11701 aaaaaacgtc atgaaaaacg gcgattgaca tataagactt ttcttgctgt agatgaacag
    11761 attcttcgtc caaatggaga aatccatttc aaaacagata atcaaggatt gtttgagtat
    11821 tctctggcaa gtttttctca atatggcatg atcctcaaac aggtatggtt agatttacat
    11881 caaagtcaat ttgaaggcaa tattatgacg gagtatgaag aaaaattttc ttcaaaaggt
    11941 caacgtattt atcgagtaga agctcaattt caagataaga ccaaaaagaa aagtctgtga
    12001 aactaacttt ctgctttcat aaaaacatta atatccataa ataaatccca gaaaatgaac
    12061 attacagatg taatgtattt tctgggattt atttgattta aaatgtattt atttcatcgg
    12121 tttttccaca aggtggaatc aattggaagc gagaaaatga aaataatcct ttgtcggtga
    12181 tcttcaattc gggtatgaca gataatgtta agaaagaaag tgtcaaaaaa ggatcaaagg
    12241 ttttagctgc tcctagttga tgtgctttag ttattaattc attcaactgt tcatagacga
    12301 tcgaaaaagg ttcctgtgtc atgattccgc ctataggaag agaaacacaa gctatttcct
    12361 gctgtttgtt gacaacggtc atcccgccac ctttttgaat caaaagatta gcagcataca
    12421 gcatatcctc gtcgtttgtc ccaacgacaa ctagattatg cgagtcatgg gcaatcgttg
    12481 tcgctatcgc tccttgtttc agctgaaatc ctttcactat gcctaatcca atgtttcccg
    12541 tttgatggtg acgctcgatc accgcaatct ttaattggtc tttttcgatt gaaggaatga
    12601 atcgttcgcc ttgtcggtcc actttttcta ccaaatcttt agtcactaga ctattcggga
    12661 taatttcgat cacatgggct tgtgaagaag aaagaagcag ctctaacgaa tgtggattta
    12721 atggttgagc gtttagtttt ggcagacaac acgtatatga atttgaatga tcagcaaata
    12781 aatggtcttg gagttgccca gattgaacaa ctaatgttcc cttatagaaa acttcatgaa
    12841 ttggcagctt ggtaagatcg tctgttaaga agaaatcagc ttgataccca ggggcaatcg
    12901 cacctagtgt tttcaaatca aaacattctg ctgcattcaa tgttgccatc tgaatggctt
    12961 gtaatggatc gattccaagt gcaatagctt gacggacgat atgatcgatt gatccttctt
    13021 ttactaaatc attgatcagt ttatcatcgg tgacaaagaa gcatcttctt gaattttcag
    13081 gtgtgatcgc cggtagtagc gatgccagat cttttgcaac ggtcccttct cgaaccatta
    13141 aatacatacc aagatccaaa cgttctttag cttcttttgc agttgtagct tcatgatccg
    13201 tccgaatacc tgctgcagga taaacattta actcttccat gccgatacca gctgcatgcc
    13261 cgtcaatttt tttcttatgc tggtgcatca attggatctt ttctaggatg tctgtttcat
    13321 tatttgctac tgcttgatag ttcatgactt cagctaagcc taaaacttcg ggtttttgta
    13381 aaaaaggggc taattttttt gcatttaaat ttgcaccatt ggtctcaaaa ggggttacag
    13441 gtacacaaga aggtagcata aaaaagatat tcatgagtgt ttgtcttgca tcttctatca
    13501 taaactcaag acctgtactc ccagcaatgt ttcctatttc atggggatct gtcactacag
    13561 tagtaactcc atgaagcaag gatacttttg aaaattcgct tggtgataaa agtgagcttt
    13621 cgatatgcac atggccatcg atgaatcctg gcactagata ttgcccatta gcattatgta
    13681 cgctttttcc ttcgtattca ccgattccta cgatcattcc ttcgcagatt gctacatctt
    13741 tttccatgat ttctttcgta aagacgttga cgatcttagc atgcttgatc actaggtcag
    13801 caggcttttt tttacttgct acttcgatat atttccttaa tgttgtttcc aatggagtgc
    13861 ctcccaagat aaatttttat aagtataaca taaattcggc ttttgccaat atttttttgt
    13921 aaattcattt tatttgaaac aaaaagatca aaaagacgta tgttttttgt ggaggggaga
    13981 ataatggaaa taaaacgatt atcggatcaa atgataagtg aaagttttgc caaacatgcc
    14041 caagacgtat atatattgcc agcttcagaa gtattttaca tctattttcg ctatggaaaa
    14101 aaacgaacat tctggaagaa agtctcttcg gcagatggac agcaactgat ctcaagattt
    14161 aagtatttgg gacagatgga tatcggagaa aaaagaaaag ctcagttagg tgcatttact
    14221 tattctttag aaaaacagga agttcgtctg cggttgtctt ctgtaggaga ttaccttcaa
    14281 cgagagagtt tagtgattcg gctattatac ggtacttttg aaacacccca tcgttgcttt
    14341 agttctcgag attttgcaac tattttacag aaaaccgtca gacgtggatt ataccttttc
    14401 agcggaccag tcggatcagg caagacttct ctaatgtatc ggttggcttt ggaagaaaaa
    14461 ttgcaagtga ttactatcga agatcctgta gagatagaag aaccgttttt tctgcagcta
    14521 cagatcaatc cgaaaatagc acagacttat gatacacttt tgaaactcgc attacggcat
    14581 cgtccagact tgctaatcat tggcgagata agagatgaat caactgcaca tgctgcggta
    14641 agagcagcac taacagggca ccgggttttt gcaaccgtac atgcacgcaa tcttgaaggt
    14701 acattggtca gaatccatga actgacaaag aaaaaagaag agctatctga atgtttagct
    14761 ggtgtcattt atcaagaact tttggaagat gccaatcacc atccttctgt tttatggagt
    14821 tatcaattta tggaggaaga atcagtaaaa aaatcatggg aggaaagtta tgaagaagct
    14881 caggaaaata tttggcagtc agaactattc taaaggatta acaagaagcc agcaaaacca
    14941 gtttgcacaa accatgggag aactgctttt atctggtttt agtctgcaag aatcccttta
    15001 tttattactc acgatccgtt cgtttccacc tgttattttg gcaaatatcc aacatcagct
    15061 aaaggaaggg caagcgttta gtgaggtact ttcgcaaata ggatatggag cagagcaact
    15121 ggtacagatc gagttagctg aacgtcatgg gaatctgccg acaactttgt tggaaattgc
    15181 caaacagttt cggcttgttg aaggatttcg caatgatttg aaaaagctgc ttgcatatcc
    15241 gatgctattg ctcacctttt tgctaggtat cttgtttagt atgcggttgt tcgttttgcc
    15301 tcagcttatt ggttcaggaa tggtcatgca agagcattgg ggtattcagc tgatacaagt
    15361 ctttcattgg tatgttctac tattcatcct agcggttact ctattggctt tactagtaag
    15421 gtatcgtctg atgaagttgt ctcctgttga tcagagtgaa tggataagca gacaattatt
    15481 gattggaccg atatattcct tgtatcaatc ggcttatttg tcactagaga ttggaaagct
    15541 ttttcatgaa ggtttcgaat taaggcaaat catagtctgc ttaaaggaaa cgaaacaaga
    15601 aagtctgatt cagcatcttg cctctaagtt gattcaagga ttggaggcag gtatgtcatt
    15661 ggctgagcag ttccagaatt atcgttttct atcagaagaa ttcagtaaaa ttgttttaca
    15721 gggagaggca aagggaagtt taggtaaaga actgattttt tacagcgaat ggacaagaaa
    15781 acagcttttc aaacgtcttc atcactggat gcattttatt cagccaatca tttttttggg
    15841 cgtagcggct ttgattttat tagtttatgc cgctgtttta ttacctgttt acggaagcat
    15901 agaggaggtt ttgccatgaa aaagagaaaa atatcttatt atagcggttt tacattgatc
    15961 gaaatgctaa tcgtcttact gatcatcagt gtattagtgt tgttgttcgt ccctaatttg
    16021 tcgcgttata ggaatcatgt agaccaagaa agtcgtgaag cgatcatcca gttggtagac
    16081 acacaaaaag agctctatgc gcttcaaaat aatggaagag ttcctactgt ggaggaattg
    16141 ttgaatgaag gctatatcaa acgagaacat gcagaaatct atcagcggcc ttgaaggtaa
    16201 ggttttagca tcaggatata cgttgtttga atcactctta ctgattttat tacttacaac
    16261 gatcctttct tttccagttc tcgcattttc tgcatggaag aatgaattgg cagtctatca
    16321 atttttcggc caatttgaaa aacgaatcta ttctacgcaa aaaatcgcta ttgtgaatca
    16381 gatccaaaca ggcttttatt ggaatagtga agagaatcaa atcatttttc atgttccgaa
    16441 tccagataaa ataaattggg aagtattaga tattcctgat gagattacct tgaaacgcca
    16501 tgcttctatt acttttgctg ctagaaccgg gaatgaaagt tcactaaaag cttatcaatt
    16561 ttattgggaa gaaaaagagc ggacgattac gtatcaattc cagataggga gtggtcgcta
    16621 tacaaaaaga attgaataaa acaggatttg ttttgttgga gtcccttaca gcgcttatga
    16681 tcgcttctgt ctgtgtctac ttagtgagct ttggccagct gtttttgctt caggcagaaa
    16741 agaaaagtgg ggaggaactg ctatttattc gaatgattta tgaagatatc aagacgcagc
    16801 ggatgtatca caaaagacca gagcgaaggg aagaaatgat cccatacgat atccaaattg
    16861 tcgataataa tggagtaatc gaaaaagtgt ttgtcaatat ggagaaagaa gcgatcagta
    16921 ttgaaaaaac aagtaattcc agcatttacg ctgattgaat gtctaattgg actgatgata
    16981 ttgagcagtc tattgctcac attcaatctt ttgattcagc aaaccatcca agtaagtgaa
    17041 tttttgcagc gtaaagatca aaaagagtgg ctaatatttt tagcacaatt agaagaagaa
    17101 ctcaagagca gtcacaatgt ctctgtgaaa aatcagcaac tgcactacac gatcgaggat
    17161 aaacagtata tcatcgaaag atatcaggca atgatacgta aaagaagaac ttccggcgga
    17221 catcaaccta tgttaacaag tatcactgag cttcaattat tggaaaaaga acacacaatt
    17281 tctttttatg tacactttga aaatggagaa gatggatatg ccatatggac aaaaaacgat
    17341 cagtagaaaa tgttataagg gctctatttt attttctgtc ttgcttttat ttcttttatg
    17401 tacgttttta ttgatggcag cattagaaag ctaccgtttg tcaatggatc ttaatatacg
    17461 aatcaaaaat tattatatag caaaaataat gaagcagatg gctttatcgg aacagtctac
    17521 gcttgccgaa agttcggaag gagtttttta ctacacaact ggatctgtaa catatcagtg
    17581 ggtacaacag tccctattct tagaggtgga agtctcaccg tttatctttc ggttcacaga
    17641 agaagtaaaa aatgatacag atactagtac agaatgatgg aatgttcgtg aaaacgccct
    17701 cttttctgtt ataacactgt ttttaataaa aaaatgtttg ccgtttcaca aataaaaaac
    17761 agtgaaaacg attgaaattt tgtcaattgt tcgatagaat taaacaggtg aaaaaattgt
    17821 tataatatat ctagaaatga ggtgcaccat gttaccagaa aaaatggaac aagggtttaa
    17881 tcaaaacctg gaagctattc agcttttaca gaatgcattg ggaacttcct ttttagaagc
    17941 ctatgtcgaa aatgcagaaa atttaataga tgattatcaa gtacgtgtgg tggatggagt
    18001 gccgacaaaa gagacaacgc aacggatcac agctctttat gaagagttga agaaactttc
    18061 tttcgagcca gaagaatggc gcagactgtc tcaattactt ttattgaagg gcagccagac
    18121 agaacatttg caggcgaacc atcaactcac tcctgatagt atcggttttt tatttgtttt
    18181 tcttattgaa caactataca caaataaaaa agcaccggtt aagattctgg atattgcggc
    18241 aggaatgggc aacttgttat taacagttct tttaaaccta tcgaatgcgg ggtaccaaac
    18301 agaaggaatt ggtgtggata ttgatgatac attgttggct gtcgcagctt ctacaagtga
    18361 tttgacacaa gcaaatgttc aatactttca tcaagacggg ttgcaggaat tattaataga
    18421 tcctgtcgat tttgcaatca gcgatttgcc aatcggttat tatccgaatg acgaaaaagc
    18481 aaaagagttt ttaacaagta ccgaagaagg ccatagttat gcacatcatt tattgctaga
    18541 gcaatcaatg aaatatgtga agccggatgg ttttggtttg tttttgatgc cgagtggatt
    18601 tttagaaacg gatcaaagtg aagaaatcaa aaaatggttt aaagaagaag gttatcttca
    18661 aggaatgatt caattgccag atgaattgtt ccgtaataaa caatcgcaaa aaagtatttt
    18721 gatcttacag aaaaaaggac cacaagcaca acaagtaaaa gaagtcttgc ttgtgaaact
    18781 tgcttcatta aaagaaccag agaaggtgac ggaatttttc aacgagttta aaaactggaa
    18841 atcatctgct ctgtaatcat tcaacgagaa ggagagtcat atgtctaaaa caattgcaat
    18901 caatgctggg agttcaagtt tgaaatggca attatatcaa atgccagaag aaaccgtagt
    18961 tgcgaaaggg atcgtcgaac gtatcggttt acaagattct atttttacca tcaagtacgg
    19021 cgataatcaa aaattcgaac aaatcatgga tatcgaaaac cacgaagtag cagtaaaaat
    19081 gctgttagat cagttgattg aactgcaaat tttaagctct tatgatgaaa tcaccggagt
    19141 agggcaccgt gttgttcacg gaggagaaac ttatggggat tcagtagtga tcgatgagaa
    19201 agtaatggac cgcattgctg aattagcaga atttgctcct ttgcataatc ctgcaaactt
    19261 aatggggatc aaagcgttta aaaagatttt acctgacatt ttgagtgtgg ctgtattcga
    19321 tacatctttc cactcaacaa tgccaaagca aaattattta tatagcttac cggtgtctta
    19381 ttatgaagat ttcaaagtaa gaaaatatgg cttccatggc acaagtcatc gttatgtgtc
    19441 ggaacgtgcg gcaaaattat tgaacaaacc gatcgaagaa ctaaaaatca tcacttgcca
    19501 cttaggaaat ggtgtatcga tcacagcagt cgatggaggt aaatccatgg atacttctat
    19561 gggattcaca ccattagctg gggtgacaat gggtacacgt tcaggtgata tcgatccagc
    19621 tgttctccca tatttaatgg aaaaattaga gattgatatt gatgaaatga tcaatgtttt
    19681 gaacaagaaa tctggtctgc taggcctaac aggattgtct agtgatatgc gtgatcttga
    19741 aaagaactat gaaaaagaac atattcgttt agcttatgac attttcgtgg accgcatccg
    19801 aaaatacatc ggcggttatg tgacaacgat gaatggtgtt gatgcaattg tctttactgc
    19861 tggtatcggc gagaatgacg gacatgtacg tagtgaaatc atcaaaggta tgtcttggtt
    19921 tggttgtgaa atcgatccag cgttgaatga attgcgtggt gaagaagtag atatctctac
    19981 aaaagaatca aaagtaagag tattagtgat cccaacagat gaggaactaa tgattgcccg
    20041 tgatgtggag cgtctgcgcg gctaagccaa agcattcatt aggataaaag atgaaacgca
    20101 tgcaagaggg aattcttgca tgtgtttttt ttgcggagaa gaccgcaaaa tggtaattta
    20161 acttcaattt cccttctttt ttacaggatc ttcttaggga attttgcttg gaaaagtggt
    20221 caaaagggaa aagactggca ttttatttat tgattttcag gaaaatccca atattttatt
    20281 ttcattcatc tttataaggg acattacata tgtaaaaaga aaaaacattt ttagatcagc
    20341 ctcgtgggct gtcgacttta ttcttcgctt tatgtttagc tagaaaaccg atccgaaaca
    20401 ttcattgatc gaataataaa aacttgattt tccacctgta ctttgcctga atcgtgaaag
    20461 cacaggtatg aaaatcaagt tttttgttgc gaaaaaacgt cctagtcaat cagttggccg
    20521 agcggctggc ggctgatctg ctcctgatca ataacgagtg cttcttggaa gtgttgctgc
    20581 aaaaagtagc ggtcatggct gactgctagg agcgttcctt gatagtcttg cagcatttct
    20641 tcaatttctt ctcgagcata gctgtcgagg tgattggttg gttcatccaa aatcaggacg
    20701 ttgatttttt ctaaaaagag tttcagcaac gccaaacgga ttttttcacc accggataaa
    20761 tctttgatcc gtttacccac atcgctttga taaaagccac ttttggctaa ttgccgtcga
    20821 gcgacttctt cgtttccagt caatgtgatg gcgtaagcca gcaaacggct atcgggatcg
    20881 gcaaactgca gctgctgcgg caaataaccg atttgcaagc tactacctaa tttgatggtg
    20941 ccaccgtaag gcttttcctc accgagtaat aatttgagta aggttgattt tcctgcacca
    21001 ttgtcgccga tgatggctaa atgatctcca cgatagactg caaaactgct gtctttgaat
    21061 agtacttgat cagcgtactg ctggtgcaag ttttgaatga tgaaaacttc tttgccagat
    21121 tttccaccat tagacaagct ttgcagccga tttttcgggg gaggtggcgg tttgacgagt
    21181 gtcagtttcg ccaggcggcg ttccagttct ttggcttttt tgaagaagga ctcattgtct
    21241 ccttcatgtg cccactgacg aaaacgacga atcatgactt tcaaccgctg gacttctttt
    21301 tgctgcagtt cgtaattttt tgtcagctca gccaagcgag cttctttcaa gactcgaaaa
    21361 cggctgtagt ttccgggata ctcaatcaat gcaccttctt ccagttccca tgtttttgtg
    21421 gtgacttgat cgagaaattg ccgatcatga gaaacgacca gaaccgattt ttttgtattc
    21481 tgtaaatagt tttccaacca gcgaatactg ttaagatcta aatgattggt cggttcatcg
    21541 agtaaaagta aatcggcatc ggctgctaaa attttggcta aagcgacttt aactcgttcg
    21601 cctccgctaa gttctgttaa ccgtgcttcc tgacggtgtg gaatccctaa gccttgcaag
    21661 atcgctgcaa tccggtcttc taatacgtaa ccgcctagtt cttcaaagcg atgctgcagc
    21721 tcaccgtaac gggccagaca cttctctaag tttgtatctg gttctgtcat caatgcttcg
    21781 tagcgactca tttgctgttt gaggtcctgt atctcagcgg cagaatggag caagtagtgg
    21841 ttgacggtcg cttcggaaac tgtaagttct tgttcgaccg tcccgatctt taagcctttt
    21901 ttgcgactga taacaccgct gtcgacacct tctctgccgg tcaagatttg taaaaaggtt
    21961 gatttgccag ttccgttgat accgactaag gcaatttttt caccagcttc aatttgacag
    22021 gataaatcct caaataatgg attcccgccg attgtttttt taatagaaat tgcttgtatg
    22081 atcatgatct ctctccttga gaccactcaa aaaggccatg gttatccatg gcctgtgtta
    22141 tttaaactca gattatcatg agaaaaatac cttgagatgg ccattgattt tacgtgaatt
    22201 caactgtttt ggataaactt gtcccgtcaa cagcaactgt aaaaccaatt gcatggctaa
    22261 atgcgatatg gtatcgttgg taattttcat tacttgttat tttagtcatg cgcatcatag
    22321 tattttccct ccttcgtgta gttgtctttt ccagtgtacc tgaaagtgtc agaaagttca
    22381 actagcgctg acatttactt aatatttatc gatgtgtaat tctgtcagag agcttttttt
    22441 atctgactga gggtaatgtc tcgtggacca catcattgta ttctgagcta aaccagcgtt
    22501 caggcgaaat catatggtgt ttgataaaaa actttgtcta catgacttcc ttttgccatt
    22561 tgcttaatac cgttgcatta aacagctctt ctctttggaa atctcaccaa catcgcgttc
    22621 aggagtatcg ttgtggttgc aatggcaagc ggtagccttt catcaattct tgaatgattt
    22681 accgaatgta gcacttgcct tctcgcggta cccgaacccg aaataaaatt ttagtttggc
    22741 gataaaatcg gtatccgcca tcgatacttc atattttttc gtatcagatt catcggaaac
    22801 ttccacattg acgaaccaat aagctttggc atgcttaggt cgttttaaac cacaccaggg
    22861 cgactaatcg ccctggtgtt cacttttttt tattttactt gaactcattg actgttttcc
    22921 ctttatttta aacattataa agaccgatga gtgcttgccg cgtcttttgt ttttatcacg
    22981 gttttttgtg caaaaaaacc tgccgatagt tggcaggtcg tctacgtgtt aagctttggt
    23041 gtggtcttta gctggcaatt ggttttctaa atcagttcgg acaacaagca catcgcaagc
    23101 tgcgtttcta ataacatatt cagaaacaga accaataaat aaacgttcta cagcattcaa
    23161 tcctgtggct cctaacataa tcaaatctac ttgattatct tctgggattt ctctggcaat
    23221 aatttgtttt ggtgagccat attctacgac agaggttaca ttattcaagc cagctttctt
    23281 cgcattgctt tcataatctg ccaatgtttg ctttgccatt tcagtagctt gttctgcaag
    23341 cattccatca aaggatgaaa cggtttggaa agaacgtgtg tcaatgacat gtgctaatag
    23401 aagttctccg ttatttcgga tagctacatt gaccgccttt ttaaaagcta attcagcttc
    23461 atcagaacca tctacggcaa ccatgatttt tttgtattgt tgtaacataa gcattcactc
    23521 ctcaagtcaa tcgtattgta ctcttttatt gtagaacact ttagggaaaa agagaattat
    23581 ttactgcttt ggttcagcaa ttttttttgc aaatcggata ctgaaggtgg cagaaacaat
    23641 aattacgatc agtaaaaaga ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat
    23701 acccattacg cctaaaacag catatgcagc accgtaaaat tgtaggaatc cttgattttt
    23761 atcatttttg ttgatcaata caaaaaagat atcctgttta ttaaataaat aatagctaac
    23821 gaataataat actaaagtgg cgataatcat taaaatccta atcatgaaca aatcctccaa
    23881 aataagcaac aaatagaaaa agcaatttgc caagagcaaa ttgctttatt ctttaaaaga
    23941 tggtaatctc cgttaatgcc gttgtttgtc ctgtagtatt gatcccgcga attcaagcag
    24001 gtgggtccca tggtcgtagc ttcgaactac caaatgataa ggcatgttac cagcagtgac
    24061 acaagacggg tcccaagata tcaataaaaa tgttcggtca acacacaaag aggacaattc
    24121 gaacatcaca gatttcccac ctctatagta gcatatgaat ttaaaaagag caatgagaga
    24181 gctcgcagaa acagaaccgt tttcttgtct agttttttgg aggatgcttt ttccactctt
    24241 ttatacggta atactgttga cccagagcct gttcatattt ccctgtatct tttggctgat
    24301 aatactgagc attcttcagt ttatctggta aatattgctg attgacccaa gcctgatcaa
    24361 aatgatgagg atactgataa cccacgccac ggttgagtga tttcgcacct ttgtaatggc
    24421 tatctctcaa atgatcagga acatctccag ctttgccctc tcgaatatca gcaagtgctg
    24481 catctaatgc catgtaggca gaattggatt ttggcgacaa gcaaagatcg acgacgacgt
    24541 ctgctagtgg gatacgtgcc tcaggcaatc ccaatttttc agcagctaat acagcgttca
    24601 ctgtacgagc cgcagcagca ggattaccta aacctatatc ttcataaccg ataaccatca
    24661 gacgccggca aatacttgcc agatcacctg cctcaactaa acgggcgaga taatgaagtg
    24721 cagcatccac atcacttcca cgaatcgatt tttgaaaagc cgaaataacg tcataatgag
    24781 catctccatt tttgtcatgg gtaagagctt ttctttggac acattcttca atgatcgaca
    24841 gtgtaagatg aatccttcct tctttatctg aaggagtcga tagtgtagcc agttccagcc
    24901 cattcaatgc actccgcaaa tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt
    24961 cgtctaactg gattgcttgt tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga
    25021 ctgccaactg gatgtcttgt tctgttaatg gtttgacttc aaagatctgt gtacggctgc
    25081 ggatcgctgg attgatggta atataaggat tctcagtagt cgctccgatc aagatgattc
    25141 ggccactttc taaatgagga agcagaaaat cctgttttgt tttatctaat cggtgtactt
    25201 catccaataa gaggatcact gtaccactca tttttgcttc ttccgctaca acttgtagat
    25261 ccttttttga atctgtagcg gcgtttagca tgcgaaatgc ataattagta gagccggcga
    25321 tcgcacttgc gatactcgtt tttccagtac caggaggtcc atatagaatc atggaagaga
    25381 gcatgcgagc atcgaccatg cggcgtatga tttttccttc cccgactaga tgctgctgac
    25441 caacgacttc atcgatcgtt cgaggtctca tgcgataagc caatggctgt tgcataggat
    25501 tttcctcctt cttgttttta cgtcccttaa ttatatcata tttagcaaga tgcctagaaa
    25561 gaacagcctt ctgttgtttt gttttcatag cttatccaac taatattgtg tatgataaag
    25621 taggtgagaa aaatgacgga taaacaaaag atagatgaat ttttttggaa taaaacgacg
    25681 ccagaagtgg cacgggattt attggggatg tatttggagc atgacacacc agaaggaaga
    25741 ttagctggat atattgtaga cgcggaagca tacctaggtc cagaagacga ggccgcccat
    25801 agctatggtt taagaagaac accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc
    25861 tacctttaca cgatgcatac tcatcggatt ttgaacatca ttactcagcc agaagggatt
    25921 cctcaaggag taatgatacg tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa
    25981 aatcgtggcg gaaaaactgg tccagatatc agcaacggtc cgggaaaatt agtggaagca
    26041 ttagcaattc ctcaagaact ttatggtcag tctatagcag atagttcgtt acgcttagtt
    26101 tttgagaaaa aaaagacacc aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac
    26161 aaaggtgtat ggacagaaaa gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct
    26221 ttacaaagaa aaaaccaaat cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa
    26281 ggatctatta acatatttag atgaaataat tgaaaaacaa gtgacagact atgatgtagc
    26341 tttagattgg gatacaaaaa atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa
    26401 taaagcacaa tttgagattg atgatgcaga agggatcgtt tcgcaagaac caattatcga
    26461 atttgaagac ggcgtacttc tttttaatcc tcagaaatct gtttttgatg aacgcaatta
    26521 tctagcagta atcccttatg aaggaaaaaa agggttggca aaatcagtag ctgattcttt
    26581 agtggaatat ctaaatgaag tattagctca agggcaaagc gatttgcttg attttttgga
    26641 tgaagacgat gacgaagctg tattcgagct tcactgggat aatcagcaac tagcagaatt
    26701 agtagaagta aaacagcaaa atgaagcaga tacttattta ccgtacccaa gttattaagg
    26761 agtgacagat atgaaatgga atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc
    26821 agttgctaat attttgatgg aggcaggggc gagtggtgtc gcgattgaag attctttaga
    26881 tgttgaacat tttcagtcgg atcaatatgg tgagatttta gataaaaatg attttacatc
    26941 gctgaaagaa ggcgcactag tcatggctta ttttccggaa acgactttct taccggaaat
    27001 cttgcctttt atcaaagatc gtatcacaca attacctgag tttggtctag ctattggaaa
    27061 aaataaagta acagttagtg aagtagagga aagcaactgg gcaacagcct ggaagaaata
    27121 ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg ccaagctggg aaaactacca
    27181 gacagaagat cctctagaaa aaattatcac actagatcct ggtatggcat ttggcacagg
    27241 cactcatccc acgacttcct tgacattgac agcactagaa acagtcttgc gtggtggtga
    27301 aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg
    27361 tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa
    27421 tatggatatg aatgaaatag ccaaagatgt ccatgtatca gctaacgatc ttttgacagg
    27481 aatcgagatt gaaagtgatg taatcgttgc gaacatttta gcagatatca tcttattgat
    27541 gatcccagat gcttggcgtt tactgaagca aacaggcacg ctgatcgttt caggaatcat
    27601 tgaagagaag aagcaaatgg ttctggatgc catgttagaa caagggttta tcgtggatca
    27661 aattttccag caaaaagact ggtatgcaat tattttgaaa aaaccagagg tggaatagat
    27721 gcagcggtat tttttaaaag aatcatatga atcaaaagac tattttgttt tagaagggga
    27781 aaactatcac cacattgtac gggttatgag gatggaaccc gaggatgaag tttttttggc
    27841 attcaatgat caaatagcca ttgttgcaga aatcacggag attactgaag aggctgttta
    27901 tctgaaagaa attaaaaaag aagaaaatca aaaggaactg ccaagttcga ttaccatagc
    27961 gagtggctat cctaaagggg aaaagctgga ttggatcgtt cagaaatcca ctgaattagg
    28021 agcgcaagct tttcttggtt tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa
    28081 acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca
    28141 tcggcagatc gtgccatcag tagagttatt ggaaacgaaa caagatttat ttgagcggct
    28201 agatgattat gacgtggtct tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc
    28261 aaatgcagcc aaaattctta gtgaaacaaa accagatagt cgaatcttag ctgtctttgg
    28321 accagaaggt ggattatccc gcgatgaagt cgattatttt gtagaacaca aagggatact
    28381 ttgtggatta ggaccaagaa tccttcgtac agaaacagca cctctttatt tattaagtgc
    28441 tgctagctat caatgggaat taatgaatcc ttcttttagc taacgttctt cattaataaa
    28501 agagtatcga cagaaagaat tgaaaaaata gtcaacaatt tgtataatag aaacaattat
    28561 ttatacaatg gatacgaaaa atcgtttgaa aaagctttta aaaagcgaac atttccgtaa
    28621 aaaaacgtat aactgcacgg attttgggac aattatcaaa cagatatttg tcccgattcc
    28681 cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac aaaagaagaa attttaacgg
    28741 gacccggtgt cataaagctc gttagtcaat acatgggtcc cgaacatgta gcatttgttg
    28801 agaaagcatg cgaatacgcg actgctgcac atgacggaca gtttagaaaa tcaggcgaac
    28861 cctacattat tcatcctatc caagtcgcag gtatattagc agatttaaaa atggatcccc
    28921 atacagtggc tacaggcttc ttacatgatg ttgttgaaga tacagaaatc actttagaag
    28981 atctgagaga agaatttggc gatgacgttg ctatgttagt ggacggcgta accaaattag
    29041 ggaaaataaa atataaatcc cacgaagagc agctagcaga aaaccaccta aagatgctgc
    29101 tagcaatggc tcaagattta cgagttatca tggtcaaatt agctgataga ctacataaca
    29161 tgcgtacgtt gaagcacctg cgagaagata agcaaagaag gatcgctcag gaaactttag
    29221 aaatctatgc gcctcttgca catcgtctag ggatcagccg gatcaaatgg gaattagaag
    29281 atacagcact tcgttatcta aatccaaaac agtattaccg tatcgtccac ttgatgcaga
    29341 cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt tgaagatatt cgaatagcga
    29401 cagaagagct ggggattttt gcagaaatct atggacggcc aaaacacatt tattcaatct
    29461 atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat ttatgacttg ctagctattc
    29521 gagtgatcgt agattcaatc aaggattgtt atgctgtatt aggagcaatc cacacaaaat
    29581 ggaaaccaat gcccggcaga tttaaggatt atattgccat gccaaaagct aatatgtacc
    29641 aatctttgca tactaccgtg atcggtccgg caggaaatcc ggtagaaatt caaatccgaa
    29701 cacaggaaat gcatgaaatc gctgaattcg gggttgctgc acactgggcc tataaagaag
    29761 gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca attaagctgg ttccatgaga
    29821 tactcgaact tcaagacgaa agctatgatg cttctgaatt tatggaaggc gtaaaaggag
    29881 atatctttag tgataaagtc tacgtcttca caccaaaagg agacgttact gagttaccaa
    29941 aaggatccgg accattggac tttgcataca gtatccatac agatatcggt aacaaaacca
    30001 ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta caaattgaaa aacggagata
    30061 tcattgagat catgacttct ccaaattcat ttggcccaag tcgcgactgg ttgaaattag
    30121 ttgctactag caaagcaaga aataagatca aacgtttctt caaagcccaa gatcgagaag
    30181 aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat tacagatcta ggatttacgc
    30241 ctaaagatat tttgacgaag aacaaactgc aagaagcact cgatcgtttt aattatcaaa
    30301 cagaagatga tctctatgca gctgtagggt atggagaagt tagccccttg acgatggcca
    30361 atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag
    30421 aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg
    30481 gcgttgtcat tcaaggggta gaaaacttac taattcgtat cagtcgctgc tgtaatccga
    30541 ttcctggtga tgatatcgtt ggttatatca ctaaaggcag agggatatcc attcatcgtc
    30601 gagattgtcc gaatgttcag cctgacaaac caaatgtagc agaacgtttg attgaagtcg
    30661 aatgggaaga tacatcgaat acacgaaaag agtatgatgc agatttggaa atttacggct
    30721 ataatcgttc aggcttattg aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc
    30781 tcaacagcgt tgaagcacgg acgaataaag ataaaatggc gacgatccat ttgacggttg
    30841 gtatccagaa tttatcccac ctaaagagta tcgtggataa aatcaaagca gtacctgatg
    30901 tctacagtgt acgccggacg aatggatagg aggacagata tgagagcagt tgttcaacgg
    30961 gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg gaaaaattgg ctacggccta
    31021 cttgttttat taggcattca tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa
    31081 aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa
    31141 gatgtgggag gacaaatttt aagtatctct caattcacac tgtttgcgga tacgaaaaaa
    31201 ggaaatcgac caagctttgt ggaagccgca cgaccagaaa cagctattcc tttatacgaa
    31261 tcattcaatg aaggaatcag aagtcgcggc atcacagtcg ctaccggaca attcggagca
    31321 gatatgtcgg tttctctcgt caatgatgga cctgtaacga tcattattga ttctaaaaac
    31381 aaataaagta atctggtttt cactagtaaa aactatgaaa cacgtcgctt aagttagact
    31441 gataaactct aacttaagtg gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc
    31501 ggaaaaaaag ttaagcttaa attatacaag ttatttttta taggaggttg ctgaagatga
    31561 aagaagtacc agtagtagat atcaaaatca cagatttaaa aaaattgtat caaaaaggat
    31621 acaacatgtt ggaggagtta cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt
    31681 tcaataaaga agctatcacg atctacggat catcagcagc aaaagtattt tatgatccaa
    31741 ggaatttcaa acgaaaaggt gctatgccaa aattagtatt gaagacactt tttggtcaag
    31801 gtggagtaca gactttggat ggagctgctc atcaccatcg gaaaaatatt tttatggatc
    31861 taatgacacc ggaaagaatg gaagattatc atcgtatctt agataagaat ctgacacaag
    31921 cacttgaagc acagcatggt caatttgaac tatttgattt aagtaagatg gttttcttta
    31981 catctatttg cgagtgggca ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa
    32041 aattagcaga atatcaaatc tctatgatca gcggaacgtt tacttctccg attgatcaca
    32101 taaaaggtgt cgaaaatcgc aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag
    32161 ctcgacaaaa tcctgtggct ggaaaagaaa atgtggcatt atatgctttt gccaatgcca
    32221 cagatttaga tggtcagctt ttacctctgg aagtggcagc tgttgaatta ttgaacatca
    32281 tccgtccaac ggttgctcta actgtgtggg cggcattgat gggacatgca cttttcagta
    32341 gaccagattt gtatcagcaa ttaaaaaatg acttttcgac gttacaagat ccgtttattc
    32401 aagaaatgag acgctattat cctttcttcc caatgctacc tgcgatttcg ctgaaagaag
    32461 tagaagtaga tggttatcgt ataccagaag gcagttgggt tattttagat ttatatggaa
    32521 cagaccatga tgaacgaaca gttgaagcgc cagattcctt tatgatcaaa cggtatgtag
    32581 ggaaagcaaa agatatttcc tataaagaag aatatgagat gattgcacaa ggcgggggaa
    32641 attttcgaca aatgcatcgc tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt
    32701 tttccgatca gctagtaaat aagtttgagt tctctgtacc tgaacaagat tggacgattc
    32761 cattcaatca gtttccgact tatccgaata gccgagcgct actttacaag aattagatta
    32821 gcataaactt atcgaaaaaa aggaagatca aacagtgctg atcttccttt tttcatatta
    32881 ctataaattg ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata
    32941 atcttcattg gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg
    33001 cttagctcca ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag
    33061 ttgcgcagga gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa
    33121 aatttctctg aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc
    33181 aaattgtttt tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa
    33241 atagggaaca aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg
    33301 ccgatggatc aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc
    33361 tttcaattgt tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt
    33421 taattcgtgc aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat
    33481 ataaaaaatg tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt
    33541 gagaaactca ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt
    33601 agcgatgacg acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc
    33661 ttcggaacgg ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa
    33721 agctgtacga actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc
    33781 tccaacggca tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct
    33841 tatttctgcc atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct
    33901 gaactaaaac gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt
    33961 ttagtaggtt ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt
    34021 acaagagagg atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct
    34081 ggagatggac gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa
    34141 atctttcaaa attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca
    34201 agggtgattt ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga
    34261 ccaaaaggaa caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa
    34321 acagcacgtt tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa
    34381 cactatgaag tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg
    34441 tatgattttt atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca
    34501 atcgttcgtt cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa
    34561 actttttata tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa
    34621 ttccatcaaa tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc
    34681 atggtgatgg cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc
    34741 aattcattag gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt
    34801 ttaccttttg aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta
    34861 cgcgtattgg acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc
    34921 ttagattatc taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat
    34981 gcattggctg ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca
    35041 catacgattt ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc
    35101 gcaggcggac gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc
    35161 ggatttgcaa tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc
    35221 cctgcattta atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa
    35281 gcattgaaag tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg
    35341 aaccgtaaag caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg
    35401 gtgatcggtg aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga
    35461 aaagaaaaaa gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa
    35521 atgactttat tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat
    35581 tagtttccga acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac
    35641 gagaccttgg gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg
    35701 tctttaatcc tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat
    35761 acgtgatcga agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa
    35821 tggccactgg tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa
    35881 ccccgccatt tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc
    35941 gttatttgga tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa
    36001 caaaagcgat tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc
    36061 tagggaaatc tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg
    36121 gacatttcta tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg
    36181 gattcgaccg ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc
    36241 gtcagcctga atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc
    36301 aaacttacac agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca
    36361 cattgccatt tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac
    36421 cggatacacg ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat
    36481 tcaaagtctt ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag
    36541 gcgctgcaga tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat
    36601 tcggagctaa ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg
    36661 ctaaattcat gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg
    36721 acttgctgat gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc
    36781 ggacacgttt aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg
    36841 taactgattg gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc
    36901 atccattcac gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag
    36961 tctatgcaga agcttatgat atcgtcttga acggttatga actaggcgga ggttctatcc
    37021 gtattcatac tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac
    37081 aaatggaaga acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg
    37141 gcggtattgc attaggattg gaccgttttg caatgttatt agccggagaa gataatattc
    37201 gtgaagtcat tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa
    37261 gtatggtttc gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat
    37321 agccgataaa aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa
    37381 ttgaaaaaca ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa
    37441 cattcgaaaa ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg
    37501 cgttgatcgc atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg
    37561 gtattactgg tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag
    37621 taccggtatc aacaactttg tatttactga atataccttt gtttgtattg gcgtggttaa
    37681 aaatcgggaa gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta
    37741 tccatatttt accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg
    37801 gggctgtgat gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc
    37861 tagatatcgt gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga
    37921 tctacttcaa tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt
    37981 tttacagtac gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac
    38041 aaaagaagat gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc
    38101 aaaactgtat gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg
    38161 ataagcagac agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag
    38221 caatgaaagc ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg
    38281 gtcgtttcta tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg
    38341 tagacagacg ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa
    38401 ctgctttttt atttgaaatt ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt
    38461 tatgggatga tagaaaaaaa tagtagagaa aggacacgat tctacttgct attttcactt
    38521 aatacccgta tattacgaat gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa
    38581 caaaaaaggt taaaaaacag ccgctacatc actgggtttg atgggctccg ttcattagct
    38641 gtggtgggtg ttatcttcta tcaccttctg ccaactagta tgaaaggcgg ctatttgggc
    38701 gttccgattt ttttcgtcgt atctggttat ttgatcacgg atctgctgcg gcaggaatgg
    38761 gaacaaaatg gaaaaatcaa tatttggcaa ttttatgtca gaagaatgaa gagactctat
    38821 ccggcacttg tttttatgct tgtgacagct tcggcttata tcactttgtt tcagcgaggg
    38881 ctgttaaaca atcttcgagg agttgtggtc agcagcttac tgtatgtcaa caactggtgg
    38941 caaatcaata acggtttatc ttatttcgat cgatttgcta atgagtcacc gtttacgcat
    39001 atctggtctt tagcagtaga aggtcaaaac tatttggttt ggccgatctt gtttgtattg
    39061 ttgatggtat ttgttcgtaa aagaaaatgg atcttttata ctgtgttagg tacatccttc
    39121 ctctccgcca tcttgatgat gatcttgtat acaccaggag gcgatccaac ccgtgtttat
    39181 tacggtaccg acactcgtct gttttctata tggttgggaa gtgcgcttgc gtttgtttgg
    39241 ccaagtacgc gtttaaagaa aaacatcccg aaacaagcga aaaatattct gaatatcgct
    39301 ggaggaatct cgcttttggc actgattctt tgcttcttct ttttagatga tcattacaat
    39361 ttcatctatt acggaggaat gctcctagtg agtctgttat gcgtagtgct agttgcagtg
    39421 acagcacacc ccggagcaag tttgaatcga tggctgacaa atccagtatt tacttggatt
    39481 ggcaaacgaa gctatggcat ttatctgtat cagtttccag ttatgatttt ttatgaagca
    39541 aaggtgactg atattgccga tcatgttttg ttgcatactt tagtggaaat cacattgatt
    39601 ctaatcatca gtgaactttc ttatcgatat gtggagcgtc cgctagcacg ttttgattat
    39661 ggtcgtacat tccaagtgat gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa
    39721 ccatgggttg ttccaggcac attacttgta gcaattgcac tcgttggttt cgttacggca
    39781 ccgaaaaacg ctgtaacagc cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa
    39841 aaattagcag aagaaaccag aaacaattca gaaaacacca ctagttcggt tgaccaagcg
    39901 atccttgata agtacgagtt gtccaaggca caagggaata aggcacaaaa attagaacta
    39961 acggcatttg gtgattcagt tatgttggat gctgcaacgg atctacaaga actttatcca
    40021 aaagtagtgg tagatggaga tgtcggacga caattatatg ccagtgtacc gtacattgaa
    40081 aaattaaagg aacaagattt gttgaaagac actgttctga ttggactagg aacgaatggc
    40141 tcattctcgg aagctcagtt tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac
    40201 tgggtcaatg tccgagtacc gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa
    40261 cagatgacga aaaaatacga caatatgacg ctgattgatt ggtacagcta cagtaatgac
    40321 catagtgact ggttttatga tgatcaagtc catccaaatc ctgaagggat gacacattac
    40381 attcacttag tcagcgaagc cttgctagga ggaagttcaa gtacgaacag cagtgagaca
    40441 aacgtgacag aatgatagct acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag
    40501 tttttttata tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag
    40561 tgtgtctgta aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct
    40621 tttgttgctt cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt
    40681 ttttgaagac gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa
    40741 agatcggttg tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa
    40801 tgttcatttg ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta
    40861 agtgatcaat ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac
    40921 taacaaaata tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc
    40981 aattcgatag tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat
    41041 agtaaagcgt ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt
    41101 cgtatgaatc tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg
    41161 cagccctttg aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac
    41221 agataagctg tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag
    41281 cgcgatacta cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc
    41341 cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac
    41401 aatcttccct tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa
    41461 agaaaacact ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg
    41521 ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac
    41581 tatttttatt aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg
    41641 accataagaa gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag
    41701 tactattagc cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag
    41761 atgagaatta catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg
    41821 tagctgcagc acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag
    41881 ttctgcgatt tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac
    41941 caagcgtcat taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt
    42001 ttcccagtgg gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa
    42061 tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt
    42121 tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga
    42181 aaacggataa agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat
    42241 tggacaaaga agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt
    42301 ttatgcgtgg gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa
    42361 acgagggaga gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca
    42421 ggtgtcatgt aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg
    42481 aatgcgtttt ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt
    42541 gattgaagac cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt
    42601 agaggcaatc gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt
    42661 agaattatac aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc
    42721 tatatcagga aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta
    42781 ttcgaactat tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa
    42841 cgtacaattt ttctttcttc cttactttga accaattgca gcgagattat attttgaaga
    42901 tgacaatatc cgaacgatca agcaagcagt agccaaggtg atagataaaa tgcaggagct
    42961 atttgattca gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa
    43021 atctgattcg gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact
    43081 tacaccgttt gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga
    43141 taatgctcgc tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa
    43201 aggcgtatgg atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc
    43261 cctgcatgat attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta
    43321 tcagtccgtc aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc
    43381 gaatatgatg aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa
    43441 cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa
    43501 attagcagaa gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa
    43561 atggctcgga gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa
    43621 aaaattacaa cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt
    43681 gacagaagca ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt
    43741 tgatgggatc accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga
    43801 gatgcgttct ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca
    43861 tcagcagatg agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg
    43921 aaacggaaca aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa
    43981 agaaataaaa caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa
    44041 tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt
    44101 tttggtttca tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt
    44161 tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga
    44221 tcatttagag caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga
    44281 accagcagca gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga
    44341 aaaacaaatc acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact
    44401 agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt
    44461 aataaacaag caaaaggaat tagcggctca aaaagacgaa atagaggaaa agaaacgatg
    44521 gattgggtat tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat
    44581 aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat
    44641 gactacgcag aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac
    44701 aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga
    44761 atccttacgt agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga
    44821 attgaatgcc gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct
    44881 tcaagaaaat ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt
    44941 aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc
    45001 gctcgcacag ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca
    45061 gctattgaaa gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca
    45121 aattgctcgt cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac
    45181 ggaacacccc atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt
    45241 agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa
    45301 aagtgaagca catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat
    45361 ggcaacgaat atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc
    45421 atttcctgag ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt
    45481 agatagacga aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca
    45541 agcagaaaag ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa
    45601 tcagctccag caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga
    45661 tagtaactgt ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa
    45721 tcaactagct gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg
    45781 gacacagctt gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga
    45841 acaagaaaga atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat
    45901 ctgtgcattt gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta
    45961 tcatgaagag caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac
    46021 tgagacattc ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat
    46081 cgaaataaag caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat
    46141 ctacgatgaa tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca
    46201 attacagcag ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg
    46261 gtatgtgctt caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg
    46321 tttaacgaaa aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa
    46381 aaccggatta gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac
    46441 cctgtctggc ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt
    46501 gatccagaca caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg
    46561 gtcattagat gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga
    46621 aggaagaatg atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca
    46681 aataagaatt gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa
    46741 agggagattg gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca
    46801 atacttgcaa gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca
    46861 tgcagaagtc ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat
    46921 agattactgc caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt
    46981 ggaactttat ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct
    47041 agaaccggta aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa
    47101 aacacaaaac aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat
    47161 tgagactaat cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc
    47221 attggcatca ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta
    47281 cacagaaaaa gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc
    47341 gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca
    47401 attattaggt acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct
    47461 cccagtagaa caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct
    47521 gcagataaat gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc
    47581 attcaaagtg gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt
    47641 tttctttatg cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata
    47701 tcaacaaacg gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt
    47761 agtgttcaag aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga
    47821 gatcggcgac gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga
    47881 atttattttc tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta
    47941 tggtgaagtc atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca
    48001 ggtgttacgt gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta
    48061 ccagaaaaat gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt
    48121 tttccgcaca gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt
    48181 gcgagagttg tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg
    48241 ttcatggctc gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt
    48301 cctgcaaagc ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc
    48361 ttttgattca gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat
    48421 acgtacagaa gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca
    48481 attggaaaaa agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat
    48541 tcatccagaa agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta
    48601 tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt
    48661 agccgatgaa atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa
    48721 agaagaacgg aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg
    48781 gcaggcagag gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa
    48841 aaaagaacgt gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag
    48901 cttaagacag gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga
    48961 ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt
    49021 acctcagcgg tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc
    49081 attgtttgca acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc
    49141 cgcagaagaa attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt
    49201 gttgaaagac ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca
    49261 aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc
    49321 tgaagcattc aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat
    49381 ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca
    49441 agtaaaagat ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca
    49501 atttacaggg atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt
    49561 ttatttaaga ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc
    49621 tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac
    49681 gggagcagat acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc
    49741 tgctggacgt gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc
    49801 tgaaggaacg attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca
    49861 aaaagtgatc caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat
    49921 gattttaagt gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt
    49981 aagaaagcct ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg
    50041 gattccattt tatcccagct tccttgctat cattttttta tttctatttt taaaggttat
    50101 acaaagattg tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa
    50161 attggagtct gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca
    50221 ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga
    50281 agcatatgaa attctttctc agccagctga aaaatctctt tttctggttt ttggaaacct
    50341 gttgcttgag aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc
    50401 agtttttttg tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt
    50461 tccttcaaga aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct
    50521 tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa
    50581 tcttttagtg attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc
    50641 attacttttg ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata
    50701 tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga
    50761 tcatacatcg tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt
    50821 cggttttcat tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa
    50881 aagctgataa ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt
    50941 tatatttcag tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat
    51001 taatcattat ggtagaagtc gattgattta ttttaatgca atctctgtgg aattttcgaa
    51061 agatttattt
//
LOCUS       DAZKCY010000007        49947 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000007 DAZKCY010000000
VERSION     DAZKCY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.007"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(242..1969)
                     /locus_tag="ACG8XC_000363"
     CDS             complement(242..1969)
                     /locus_tag="ACG8XC_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFP9190089.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1962..3155)
                     /locus_tag="ACG8XC_000364"
     CDS             complement(1962..3155)
                     /locus_tag="ACG8XC_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9190090.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3342..3986)
                     /locus_tag="ACG8XC_000365"
     CDS             complement(3342..3986)
                     /locus_tag="ACG8XC_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9190091.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4080..4214)
                     /locus_tag="ACG8XC_000366"
     CDS             complement(4080..4214)
                     /locus_tag="ACG8XC_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFP9190092.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKKQIKR
                     "
     gene            complement(4216..6348)
                     /gene="feoB"
                     /locus_tag="ACG8XC_000367"
     CDS             complement(4216..6348)
                     /gene="feoB"
                     /locus_tag="ACG8XC_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFP9190093.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6345..6818)
                     /locus_tag="ACG8XC_000368"
     CDS             complement(6345..6818)
                     /locus_tag="ACG8XC_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFP9190094.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7219..7443
                     /gene="nrdH"
                     /locus_tag="ACG8XC_000369"
     CDS             7219..7443
                     /gene="nrdH"
                     /locus_tag="ACG8XC_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP9190095.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7602..9761
                     /gene="nrdE"
                     /locus_tag="ACG8XC_000370"
     CDS             7602..9761
                     /gene="nrdE"
                     /locus_tag="ACG8XC_000370"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9190096.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9811..10776
                     /gene="nrdF"
                     /locus_tag="ACG8XC_000371"
     CDS             9811..10776
                     /gene="nrdF"
                     /locus_tag="ACG8XC_000371"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9190097.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10865..12004)
                     /locus_tag="ACG8XC_000372"
     CDS             complement(10865..12004)
                     /locus_tag="ACG8XC_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9190098.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12313..13026)
                     /locus_tag="ACG8XC_000373"
     CDS             complement(12313..13026)
                     /locus_tag="ACG8XC_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP9190099.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13280..13981)
                     /gene="nagB"
                     /locus_tag="ACG8XC_000374"
     CDS             complement(13280..13981)
                     /gene="nagB"
                     /locus_tag="ACG8XC_000374"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9190100.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14215..15747
                     /locus_tag="ACG8XC_000375"
     CDS             14215..15747
                     /locus_tag="ACG8XC_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9190101.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15938..17140)
                     /locus_tag="ACG8XC_000376"
     CDS             complement(15938..17140)
                     /locus_tag="ACG8XC_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9190102.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17222..17911)
                     /locus_tag="ACG8XC_000377"
     CDS             complement(17222..17911)
                     /locus_tag="ACG8XC_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9190103.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18017..18385)
                     /gene="rplL"
                     /locus_tag="ACG8XC_000378"
     CDS             complement(18017..18385)
                     /gene="rplL"
                     /locus_tag="ACG8XC_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP9190104.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18438..18941)
                     /gene="rplJ"
                     /locus_tag="ACG8XC_000379"
     CDS             complement(18438..18941)
                     /gene="rplJ"
                     /locus_tag="ACG8XC_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP9190105.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19145..19272)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19351..20040)
                     /gene="rplA"
                     /locus_tag="ACG8XC_000380"
     CDS             complement(19351..20040)
                     /gene="rplA"
                     /locus_tag="ACG8XC_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP9190106.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20144..20566)
                     /gene="rplK"
                     /locus_tag="ACG8XC_000381"
     CDS             complement(20144..20566)
                     /gene="rplK"
                     /locus_tag="ACG8XC_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP9190107.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20909..21577
                     /gene="sdaAB"
                     /locus_tag="ACG8XC_000382"
     CDS             20909..21577
                     /gene="sdaAB"
                     /locus_tag="ACG8XC_000382"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFP9190108.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21590..22462
                     /gene="sdaAA"
                     /locus_tag="ACG8XC_000383"
     CDS             21590..22462
                     /gene="sdaAA"
                     /locus_tag="ACG8XC_000383"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFP9190109.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22553..23653)
                     /locus_tag="ACG8XC_000384"
                     /pseudo
     CDS             complement(22553..23653)
                     /locus_tag="ACG8XC_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(23745..24632)
                     /locus_tag="ACG8XC_000385"
     CDS             complement(23745..24632)
                     /locus_tag="ACG8XC_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9190110.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(24709..25254)
                     /gene="nusG"
                     /locus_tag="ACG8XC_000386"
     CDS             complement(24709..25254)
                     /gene="nusG"
                     /locus_tag="ACG8XC_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP9190111.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25355..25525)
                     /gene="secE"
                     /locus_tag="ACG8XC_000387"
     CDS             complement(25355..25525)
                     /gene="secE"
                     /locus_tag="ACG8XC_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP9190112.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25549..25701)
                     /gene="rpmG"
                     /locus_tag="ACG8XC_000388"
     CDS             complement(25549..25701)
                     /gene="rpmG"
                     /locus_tag="ACG8XC_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9190113.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25863..26507
                     /gene="cbpA"
                     /locus_tag="ACG8XC_000389"
     CDS             25863..26507
                     /gene="cbpA"
                     /locus_tag="ACG8XC_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFP9190114.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26563..27066)
                     /locus_tag="ACG8XC_000390"
     CDS             complement(26563..27066)
                     /locus_tag="ACG8XC_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP9190115.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNTFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27079..27125)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27663..28628
                     /locus_tag="ACG8XC_000391"
     CDS             27663..28628
                     /locus_tag="ACG8XC_000391"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9190116.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28727..29899)
                     /locus_tag="ACG8XC_000392"
     CDS             complement(28727..29899)
                     /locus_tag="ACG8XC_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9190117.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29886..31031)
                     /locus_tag="ACG8XC_000393"
                     /pseudo
     CDS             complement(29886..31031)
                     /locus_tag="ACG8XC_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(31045..31977)
                     /locus_tag="ACG8XC_000394"
     CDS             complement(31045..31977)
                     /locus_tag="ACG8XC_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190118.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            32094..33173
                     /locus_tag="ACG8XC_000395"
     CDS             32094..33173
                     /locus_tag="ACG8XC_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9190119.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQILEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33170..33799
                     /locus_tag="ACG8XC_000396"
     CDS             33170..33799
                     /locus_tag="ACG8XC_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9190120.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(33909..34136)
                     /locus_tag="ACG8XC_000397"
     CDS             complement(33909..34136)
                     /locus_tag="ACG8XC_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9190121.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34239..36827)
                     /locus_tag="ACG8XC_000398"
     CDS             complement(34239..36827)
                     /locus_tag="ACG8XC_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFP9190122.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(36847..37506)
                     /locus_tag="ACG8XC_000399"
     CDS             complement(36847..37506)
                     /locus_tag="ACG8XC_000399"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9190123.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37555..38058)
                     /gene="trmL"
                     /locus_tag="ACG8XC_000400"
     CDS             complement(37555..38058)
                     /gene="trmL"
                     /locus_tag="ACG8XC_000400"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP9190124.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38279..39145)
                     /locus_tag="ACG8XC_000401"
     CDS             complement(38279..39145)
                     /locus_tag="ACG8XC_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP9190125.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39254..39895
                     /locus_tag="ACG8XC_000402"
     CDS             39254..39895
                     /locus_tag="ACG8XC_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP9190126.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(39957..41318)
                     /gene="mgtE"
                     /locus_tag="ACG8XC_000403"
     CDS             complement(39957..41318)
                     /gene="mgtE"
                     /locus_tag="ACG8XC_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFP9190127.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41336..42235)
                     /locus_tag="ACG8XC_000404"
     CDS             complement(41336..42235)
                     /locus_tag="ACG8XC_000404"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9190128.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42237..43034)
                     /locus_tag="ACG8XC_000405"
     CDS             complement(42237..43034)
                     /locus_tag="ACG8XC_000405"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFP9190129.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLYETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43012..43716)
                     /locus_tag="ACG8XC_000406"
     CDS             complement(43012..43716)
                     /locus_tag="ACG8XC_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFP9190130.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            43847..44422
                     /locus_tag="ACG8XC_000407"
     CDS             43847..44422
                     /locus_tag="ACG8XC_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFP9190131.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            44540..45217
                     /locus_tag="ACG8XC_000408"
     CDS             44540..45217
                     /locus_tag="ACG8XC_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFP9190132.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45296..47104)
                     /gene="pepF"
                     /locus_tag="ACG8XC_000409"
     CDS             complement(45296..47104)
                     /gene="pepF"
                     /locus_tag="ACG8XC_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFP9190133.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTSVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47280..48278)
                     /locus_tag="ACG8XC_000410"
     CDS             complement(47280..48278)
                     /locus_tag="ACG8XC_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFP9190134.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48504..49157)
                     /locus_tag="ACG8XC_000411"
     CDS             complement(48504..49157)
                     /locus_tag="ACG8XC_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFP9190135.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(49489..49887)
                     /gene="spxA"
                     /locus_tag="ACG8XC_000412"
     CDS             complement(49489..49887)
                     /gene="spxA"
                     /locus_tag="ACG8XC_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFP9190136.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        14929 a        10492 c         8802 g        15724 t
ORIGIN      
        1 tttttgagaa tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg
       61 atcgatctct ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttggacgtt
      121 tcgaagatga gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga
      181 gcttccaaat ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt
      241 tttatttttt ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt
      301 gctgtggatc ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc
      361 aatggaaatt gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa
      421 ccatcgtatg gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca
      481 gatcatattt ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt
      541 ttttgatcgc ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc
      601 agtctttcaa gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc
      661 ctggctcgat tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta
      721 catccatgta ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt
      781 cttcgacttg atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg
      841 aaaccatggc atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg
      901 gcgtcatcgt tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag
      961 caacttctgt atcccattca acataagata agttgatacg ttttgtttct ttggcagatg
     1021 aaaaactccc tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc
     1081 cttgacgttt tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt
     1141 tcaaatgctg tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga
     1201 caaatccatt cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg
     1261 gtgcaccgat catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga
     1321 tccctgccat gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac
     1381 ttgtgctccc gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta
     1441 aattcgtaaa acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa
     1501 ccatcccgat accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct
     1561 gcataaagtc aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga
     1621 tccctaatgg taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag
     1681 tattcatcaa atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga
     1741 acaacgtaag tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga
     1801 taaacaaagg agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc
     1861 caatcgtttg taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat
     1921 tttctaccca gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc
     1981 tcgcttgttt ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt
     2041 ccatcctcta caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt
     2101 accaccatgt ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt
     2161 ttttgcttcc tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt
     2221 ctgtcaacag ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt
     2281 ggtccatcag attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc
     2341 actttggaac gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc
     2401 aatatttctt cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg
     2461 tccccaatcc gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca
     2521 ttggcttgca ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc
     2581 gagaacgctt cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca
     2641 acccgttgtt gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa
     2701 ctagaatttt ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg
     2761 acttctaacc cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt
     2821 tggaaaacca tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc
     2881 gatacatctt ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat
     2941 agacggatca atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct
     3001 tcttttacgt caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc
     3061 tccgttttcg gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat
     3121 atctttgtta aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat
     3181 tcatttttct ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca
     3241 aaaatgaaag aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa
     3301 aaggtttagc caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc
     3361 tccgggtaaa aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg
     3421 atgtcccctg ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga
     3481 gaaattgcga taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg
     3541 tccagatgca ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta
     3601 tgtattcgct tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca
     3661 tcttgttgct tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca
     3721 tcttgatatt gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt
     3781 ccatgccgta cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc
     3841 tcaggagaag cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact
     3901 ttatatcggt tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg
     3961 cggacgattt ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat
     4021 cttactatgc tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat
     4081 catcgcttga tctgtttctt ttttactggg caagctgttt gacagtcatc acaattctta
     4141 ccggatttta aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat
     4201 aagaatgtcg ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt
     4261 ggattttggc tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata
     4321 cgtccctaac gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact
     4381 aactaaataa gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt
     4441 catttcccga tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc
     4501 taacaatgaa taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct
     4561 gccattttcg gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc
     4621 tttagcgacc aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg
     4681 ctgaaaaaca actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac
     4741 aggttgagca taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat
     4801 aatcgttcct gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc
     4861 tgaccattga ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc
     4921 aaacaaacgt gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa
     4981 ataggcagaa ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat
     5041 aatcggcaac tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc
     5101 ttgctcgttt tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat
     5161 cagcatagga atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac
     5221 aaaagcgatc ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat
     5281 aatcaattgc ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat
     5341 cagctcctgc atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg
     5401 taaccatgta ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg
     5461 aatagccaga taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg
     5521 attcgttgca atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac
     5581 aacacagagt gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc
     5641 actatcgtcg tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg
     5701 agaaactgct atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc
     5761 ataccagcgt ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc
     5821 ttctaaacgt ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc
     5881 ctgaatcttt tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac
     5941 tggtacacca agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat
     6001 gacatccatc atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt
     6061 taaataaaga tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg
     6121 ttcctgaacc aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata
     6181 gatgccgggt aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt
     6241 ttctactgtt acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa
     6301 caagctagtt ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc
     6361 ctttgtatca agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact
     6421 gtcagttcga ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc
     6481 gtcaatccca tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg
     6541 atcccagtct ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc
     6601 tttttattga caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt
     6661 accagtaaaa tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg
     6721 atcccgatat tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga
     6781 taaacgaaat tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat
     6841 aaatcatgta ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa
     6901 atgagaatca ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga
     6961 tattatgagc gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag
     7021 ttttccacaa ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc
     7081 tcttttttca aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt
     7141 ttgttgtttg acttttttac taagagtttt atactatcta ttatagataa taagaatgtg
     7201 ttagaggagg cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt
     7261 aagatggtca aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat
     7321 gaacagcctg aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt
     7381 acttctgatg cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc
     7441 taacatgact aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca
     7501 gtttttgtcg caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca
     7561 atcaagcatc atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac
     7621 aagatgtcag ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc
     7681 ctttgaataa ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata
     7741 cgatgacttt tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag
     7801 aagcagaatt cattcaattg tattcaccat catttattga aaagctatat caatttttgg
     7861 acgagcaaca tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg
     7921 cgttgaaaac caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca
     7981 atgcgcttta ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga
     8041 tccatcaacg atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg
     8101 gcgaattggt gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct
     8161 caatcaactc tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa
     8221 atcttcgtga agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc
     8281 cagtcatgaa attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag
     8341 gagctggcgt ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa
     8401 aaaaagaaaa tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac
     8461 ctgataaatt ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata
     8521 gtgtcgaaaa agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc
     8581 atctagttgc aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg
     8641 aaatctcaaa attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga
     8701 accgcgagaa tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac
     8761 aagttcaaac gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata
     8821 tctcatgtaa cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc
     8881 aatctgtgcg tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg
     8941 tcgtaccacc gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg
     9001 gcttacacac ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact
     9061 ttacaaatat ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg
     9121 ctcgtgaaag aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt
     9181 atttcgaaaa atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt
     9241 ttgaaggcat ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga
     9301 aagatggact gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata
     9361 tcaacgatac gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga
     9421 agaaaatcgg taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt
     9481 acacttctgc ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac
     9541 aacacgtcga tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca
     9601 tttatgaatg gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt
     9661 acgctttcca caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag
     9721 aagaaatcgg aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct
     9781 tattcatatt ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac
     9841 tggaatgaaa ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt
     9901 tggctagata cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa
     9961 ttggaaaaag atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa
    10021 tctgaaagtg gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt
    10081 ttgaacaata ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt
    10141 acactgaata cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat
    10201 ctgcaaaaaa aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag
    10261 aaaattgcca gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt
    10321 tattatttag gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga
    10381 gatgaatctg tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta
    10441 cctgaagcag aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac
    10501 gaaaacgaag aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc
    10561 aagacattcc tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc
    10621 tttccagata ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca
    10681 agcaaccatg acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg
    10741 atgaaagacg acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt
    10801 ttcgaaaaat aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt
    10861 atgcttattc acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt
    10921 ccattcttct caataaatag attttccgga tatttaatat tatcgtctta atcactgcat
    10981 agacaggtat gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata
    11041 ccaagatgat cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca
    11101 aatttccatc taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg
    11161 gagagataaa caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg
    11221 ggataatatt cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata
    11281 ttctcattgc caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga
    11341 tataggcaga taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg
    11401 ctttcggaaa gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata
    11461 gcaaaataaa tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt
    11521 ttgagacact tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat
    11581 ttgctgatgc taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt
    11641 catttaacca tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg
    11701 tctgttccat taactgaggc actacattca tgacagttaa aacaactaaa atgattacga
    11761 ccaaaaacgt caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa
    11821 cgattggatc gaacatataa tagataaaac cagcaacaag caatggtaaa aaaactgccg
    11881 aaagcatttt caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga
    11941 tgatcgtgag tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt
    12001 gcatacatct cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg
    12061 cgctgagatt ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga
    12121 cgagtactta aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt
    12181 ttccaaataa acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa
    12241 tataaggaac tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca
    12301 acctcagatc atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac
    12361 ggacaactcc tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat
    12421 atttttgtac actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac
    12481 gatatttttc tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa
    12541 ttggatttct aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc
    12601 aatagcaatc ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat
    12661 ccctaatcca gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt
    12721 cgtgacgatg atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag
    12781 ccttgcttca tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa
    12841 tgcaccacta gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa
    12901 aatatgcgta cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc
    12961 gccaaatagc atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc
    13021 attcatctaa acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt
    13081 gcaattttta cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta
    13141 tgaataaacg gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg
    13201 ggagctattt ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
    13261 tttagcctac tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa
    13321 cattgtcgtg attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca
    13381 tccctttgat tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt
    13441 tcataattga accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga
    13501 agaatcgttt gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt
    13561 caaatgaagc accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt
    13621 ggatatcgat tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat
    13681 cttctgcttt tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga
    13741 catccatgaa atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta
    13801 agtttacaga agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag
    13861 taactggtgt gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt
    13921 tgatgatttc aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca
    13981 tagtgattgc tcctttatat tttggtatag cccaattata gcattctttt tttcttttta
    14041 caataattag tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc
    14101 gtgttaggtt tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag
    14161 tgattcactg tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt
    14221 atactgacat taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc
    14281 gatctgcaat taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa
    14341 agtacattga ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg
    14401 caaaaaaata ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca
    14461 atcgaagaat ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct
    14521 tttctttacc aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa
    14581 ctccaaacgg aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg
    14641 agcagcgggt tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt
    14701 ggacaacgtt caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc
    14761 ttagatgaac caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt
    14821 caggattttg acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc
    14881 accacccata tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa
    14941 caagcattga aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa
    15001 caaaaacaaa tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca
    15061 acgatggcaa aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct
    15121 tcagcaagta aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg
    15181 gaaacaactg atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc
    15241 atccgctttg gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta
    15301 ttaaaaacac tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac
    15361 acaaaaatca attacttttc acaagatctg tactgggaaa atccattaga aacaccacta
    15421 caatatttaa gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct
    15481 aaagcagggt tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa
    15541 caaactaagg tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat
    15601 gaaccgacga accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa
    15661 tttccaggaa cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa
    15721 cgagtcattg agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg
    15781 tgacaaaagt tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt
    15841 cggtattagc tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt
    15901 acttgatgca gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc
    15961 tctttccttt aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc
    16021 taaccgttac acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag
    16081 ccgcacctcc aataaagtac ataaacataa atacagtatt atttcggttg cttgctgctt
    16141 cacctagccc ttgtactcgt gtttgattgg cgacttgacc aaattgattc ccgacgtcta
    16201 ataaaataat agctaaaagc aaaatggcca aatggcttcc gccaaccaat aaagcaataa
    16261 agcttattgt ttgcataaca agtcccatat acacgatttt tctttcagaa taagaatctg
    16321 ccagtcgtcc cacgataggt gcagcaactg ctccagacaa accaaaaatc gctaatatcc
    16381 ctgcttcgaa tgttccccaa ttgtaggcgg gactgctgat ataaaaaatc aacgtagacc
    16441 aaaagatcga aaatgttcca aacatgaaaa atccactgac tgccgcttct cttaatagac
    16501 gttgagaagc aactaaatgc ggcaaacttt taagtgaatc aaggtatgaa acagctgtac
    16561 tactttgctg gacagaacgc ggcattttcc agtaaagcag aagtgtaaga attccgactg
    16621 cacataaagc aaataaatag atcatccgcc aagaaaaaag actggcaaca ataccagata
    16681 ccgtccgaga aagcaaaatc cccacgagta aaccgctaag caatgtaccc atgaccttcc
    16741 ctcttgcttt tggtcccgct agtaccgcac cgtaaggaat gatgatttgc ggcacgatcg
    16801 aaagtaatcc tattaaaaaa gaagcgcatg caaataacgt aaacgaaggc gcaaaaaaag
    16861 cggccaacag agagacggca gataacgctg ccattcgaat gatcaatttt ggacgatcca
    16921 ccacgtcccc taaaggaact aaaaacagta accctaacgc atagcctaat tgggtcaaca
    16981 tcgtcaatat cccgactgca cttgtgctaa cagataaact agctgctact tttgttccaa
    17041 tcggctggat gtaatacata ttggcaacaa caacgccaca agtgattgat aatagtaaaa
    17101 taagcgaagt tgttaaaggt tttttttcat tactcttcat tgatttcccc catttcctat
    17161 cttagtttac aacttatgga aaagattgga aatagaaaaa tacaactatt cgccacactt
    17221 attatttgtg cttttgtttt ccttgttttt tcttgcgatc tttataaatg aagacaacta
    17281 accctactag cacagtaaca cttgcaacca acgtgactgc agttttgtta tatccgtgaa
    17341 acagagcttc tccaccacac gcatacaaca cagacgaagg gatgactcca atcacgatcg
    17401 acaatatgat ttgctgtaga ggcaatttca tcgcatctgc agcaaaattg accatagaag
    17461 atggaataac cggtatcata tagcctaaca ttacacctat ttttggatgt ttcatttgtc
    17521 ggattgcttg cacccaatga ttcgtttcgg ttttcttatc taaaaatttt aagcgatgca
    17581 taaggaaaat tgcagtgatg ttaccaagag cattacccaa aatgttgatg atcgcacctg
    17641 tcagtggtcc aaagctcaaa ccagcaatga ctccaactac tgaagtagga atacctggaa
    17701 cagcacacat caccgctgtc aatacgatca agagtatcga tgcaaaagtc ccatgcgaac
    17761 gaatatgtgc gagtagctct gccttactcg ctctcggatt taaaaataat tggatgtctg
    17821 tctgatattc taaatacaac cgatagccca ctaccagtat cagaatgatt cccaacgaga
    17881 tcaatatcaa tcggattatt ttctttttca tatttcctcc tgaaaaaaat ctatattcat
    17941 taaataatag tttaacataa aaacaaaaaa gcaccgaaat ccaacatagg atctcagtgc
    18001 ttttaaatag taaaaattat ttaacagtta cagatgcgcc aacttcttca agtttagctt
    18061 tcaattcttc agcttcttct ttagatacgc cttctttaac tggtgcagga gcgccatcaa
    18121 ctacagcttt agcttctttc aagcctaaac cagttgcttc acgaactgct ttgataactt
    18181 taactttttg gtctccagct gcagttaatt ctacagtgaa ttctgtttgt tcttcagcag
    18241 cagcagcgcc accagcagca gctacagcta caggagcagc agcagatacg ccaaattctt
    18301 cttcaatagc tttaactagg tcgttaagtt ctaggatagt tgcttctttt aactcagcaa
    18361 cgatgttttc aatgttcaat gccattataa tttcctccgt ttaattgttc ttttttattt
    18421 aatgctttat gcggcaatta agctgcatct ccatcttctt tgtctgccac tgctttgaca
    18481 gcgtaagcca cgttgcggac tggcgcttgt agtacagata gcaacataga tagaagtcct
    18541 tcgcgatttg gcaattttgc caatgctgtg atttcttcca atgaagagac tttaccttcg
    18601 atgataccgc ctttgatttc taatgctttt gcttctttag caaattcgtc catgattttc
    18661 gctggtgcga ctacatcttc gttactgaat gcaacagctg taggtccagt gaatacttcg
    18721 tccatgcctt caaggccagc ttttttagct gcacgtgaaa gaatagaatt tttgataact
    18781 ttcatttcta cgttagcatc acgaagttgt ttacgtaaac gtgttacttc atcaactgtt
    18841 aatccgcggt agtcaacgac tactactgat gcagctgctg taaatttttc gcttacttca
    18901 tcaacgattg ctgctttttt tgcgataatt gcttcactca tttttgagtt tcacctcctg
    18961 aattttgatg gaagagtttt ttgcactgaa aaggcaagtc acttttatag ttactttgct
    19021 caaccagtat tattcatcac ctattcactt cgttcatagt gattctactc gctttgctcg
    19081 aaaggaaact cctttcgctt tgctcaacca gtattgttca tcatctcttc acttcgttca
    19141 tagtgattca caataaaaaa ctctatgcca ccgcagacat agagggacta ttgaatatat
    19201 tcaataaacg tcctcggtag gaaattaagg cttgatgcca cctactgtct tcggtacaag
    19261 ttaattatta acctcaacta gcttagcatg ctaaactagt tgagtcaaga attatttttc
    19321 agttggatca atcaacattc taatatttaa ttagaaagaa gcttggtcaa catggatacc
    19381 aggtccaaat gttgttgtga tcgttacatt tttcatgtat tgacctttag ctgctgatgg
    19441 tttagctttt agcaatacat catggattgc tgcaaagttt tcaactaatt tttcatcgtc
    19501 aaatgaaact tttccgattg gtacatggat atttccagct ttgtccacac ggtaagttac
    19561 tttaccagct ttcacttcgt ttactgcttt tgttacgtcc attgtaactg taccagtttt
    19621 agggtttggc attaagcctt ttggtcctaa tactcgtcct agacgaccta cagtagccat
    19681 catatcaggt gttgcaacaa ctacatcaaa gtcaaaccat ccgttttgga ttttttgaac
    19741 catgtcgtcg tcacctacga aatctgcacc agctgcttca gcttcttttg ctttgtctcc
    19801 tttagcaaaa actaaaacag tttgtgtttt accagttccg tttggtaata caacagcacc
    19861 acggatttgt tggtctgctt ttttagggtc tacgttcaaa cggtatgcaa cttcaactgt
    19921 tgcatcaaat tttgcaatgt ttgtttcttt tgctaaagct actgcttcgg caacaggata
    19981 aactttactt gaatcaactt tttttaatgc atcttgcatt tttttgctct ttttagccat
    20041 tttggttcct ccttgattgt ggttataacg gttgtacctc ccacgtgttc tatatctttc
    20101 aatatagagc caactgagaa gctgctgtct tagggtgtat agattattct acagtgatcc
    20161 ccatgcttcg tgcagttcct tcaaccatgc gcattgctgc ttcaacgtta gctgcgttta
    20221 ggtcttccat ttttaattca gcgatttctc ttacttgatc gctagaaact ttcgcaactt
    20281 tatttttgtt tggttcgcct gaaccttttt cgattttagc tgcttttttc aatagaacag
    20341 cagctggcgg tgtttttgta acgaatgtga atgaacggtc ttcgtataca gaaataacaa
    20401 ccggaatgat caaacctgct tgatcagctg tacgagcgtt gaattctttt gtgaatccca
    20461 tgatattgat acccgcttga cctagtgcag gacctactgg tggagctgga gttgctttac
    20521 ctgcaggaat ttgcaattta acgatttttt ctactttctt tgccacgaga catacctcct
    20581 taagtccgtg atgtggttaa ttggagatgc atatttctcc tcccacgttt tatctgatct
    20641 gaaaaaatcc agacaaataa tgtgcaaaac aatgcacact ggtacagtat agcaaattta
    20701 tttgtttttt caagattttt ttctgctatt tttacatttt gcttgaataa acgctaaatt
    20761 tattctaaaa aaacaacaat ttttcgttct catttcttct tttatgttac acttgcgcta
    20821 gcatggttca acagatgaaa gcaaacaata acacggtttt ttagaccctt gtacaaagat
    20881 tcctacataa caaaatgagg tggagaaaat gaaagattta aggtatagaa gtgtctttga
    20941 tattattgga cctgtgatga ttggtccaag tagctctcac actgccggag ctgctagaat
    21001 cgggaaaatc atacgtagta tatttggcga acagcctgat tctgtagata tttatctata
    21061 tgaatcattt gcaaaaacct atcgggggca cggaacagat attgcattag ttggcggcct
    21121 gttagggatg gaacccgatg atgaacgttt agctgattct ttgaaattgg catatgaagc
    21181 cggtatggaa gtcttgtttg ttcctaagag tgaaaaagcc gaacatccaa actctgtaaa
    21241 gatgcttcta aaaaaaggaa cacgaaagct ttcagtcact ggcatttcga tcggcggtgg
    21301 caatatccag atttctgaat tgaatggatt caaaatttct ttaagcatgg gtactcctac
    21361 cttgatcgtt gtccaccaag atgttccagg tatgattgct aaagtgacta gtatactttc
    21421 tgagaccaat acgaatatcg gcactatgac agtgacacgg gaatcaaaag gtgaaaatgc
    21481 cattatgata attgaagtag atgatcctca agtagaagaa acggtaaaaa gactcaaaca
    21541 gctgccaaat atcgacagcg taaattattt cgattaagga gctaaaaaaa tgtttttatc
    21601 gattgaagaa ttagtagatc aagcaaaagc ttatccaagt gtagcagaat tgatgattgc
    21661 agttgaaatg gaaatgagtg gccgttcccg tagtcacatc atcgagatca tggaaaaaaa
    21721 cctctcagta atggaacaat caatcaacga gggaaaagaa ggtgttacct ccgtcacagg
    21781 aattaccggt ggtgatgcaa aaaaattaga tgattattta aatgaggcag actttttaag
    21841 tggagaaaca atcttaaaag ctgtacgcaa tgctgtagct gttaatgaag tcaatgcaaa
    21901 aatggggttg atttgtgcta caccgactgc cggaagcgcg ggtgttgttc ctggtgtgct
    21961 gatggctgtc cgagatcggc ttgatttgac acatgaacag caattggaat tcttatttac
    22021 cgctggcgct ttcgggttag tcatcgcaaa caacgcttct atcagtggtg cagaaggggg
    22081 atgtcaagca gaagttggtt ctgcaagtgc aatggcagca gcagctttgg tttgtgcaaa
    22141 aaatgggact cctcatcaag cagcacaagc agtagccatt acattgaaaa atatgatggg
    22201 actgatttgc gatcccgttg caggcttagt tgaagtcccg tgtgtcaaac gtaatgcttt
    22261 aggctcttct caagctttta tctctgctga tatggcatta gcgggtatcg aaagtgtcat
    22321 tccgccggat gaagtcgttg ctgcaatgta ccaagtcgga cgtcaaatgc cgtcgatttt
    22381 caaagaaaca gcagaaggtg gcttagctgt tacgccaact gctaaacgac ttgcggaaga
    22441 gattttaaat aagagggcgt gataaagctc cttaatttcg agtattaaga gattagttat
    22501 cttcataaca aaaaacgaag agaggactaa ttgcccttct cttcgttttt tattaatcca
    22561 tcaatttcgt attttttact tcatcgatcg tttttgttcc tgcaagttgc atcgtgatcg
    22621 acaattcttt gttcaggtgt tcaaaaacag atgttactcc ttctgcccca cctagattca
    22681 acccataaat cactggtcgg ccaatcgcaa ctaagtcagc accacttgcc agagctttga
    22741 agacatgctc tcctcgacgg acaccagagt cgaaaatcac tggtacacgt ttgttgacta
    22801 cttcggcaat ttttggcaat acttcaaagg acgcaggacc tccatctaat tgacgtccac
    22861 catgattgga tacccagata ccatctgccc ctgcagagat agcgattact gcatcttctg
    22921 gcgattgtat tccttttacg ataactggaa gatgggtgat ttctttgatt gttttgatgt
    22981 catctggtgt caagccttgt tttgcagctg cataaatttc tgcgattcct tttccttcgc
    23041 cattaccact tgcactttgt tcgctatacg cagctaagtt aggcattggt aatggaaatt
    23101 ggaattgatt gatgacgtct tcttcacggt aaccaccgag cgttgaatct gccgttaaaa
    23161 tgatagcttt agcccctgct tctactgctt tgttcaaaat gaactcattg aatctgtcgt
    23221 ctttactcat atataattgg aaaaactgcg gtgcgcctgg cgctgcatcg gctgcgtctt
    23281 tgattgtcgt atttgcataa gtgctgatgg aaaagattga tccagcagca gctactcctt
    23341 tagctgtatc agcttcccct ttttcatgtg ccaatccttg ggccgctgat ggcgcttgga
    23401 taatcggtgt atctaattca attccgaaaa cagatgtatg caaatcggct gagtcgattc
    23461 cgcgtaatat ccgtggcatg attttctttg tattaaaaga agtcgtattt tctttcatcg
    23521 tccactcatc ttcagaacca ccgcgaatat agccaaaagc tcctgcttcc atgcgctctt
    23581 ttacacgacc ttctagttag gcaatgttta caatatcgat tggatgttct tcattacttg
    23641 tatgataagt catagtagat ccttctttca tcttcttgat tacatataaa tcatactaaa
    23701 cttacaaaaa gtaagcaaac atttactctc agatactttt atcgctattc tttccaaata
    23761 aatcgactta caagctgatc tacttcttgg ttttcatgaa gcatactatg agaggcattt
    23821 tcacctgtca ctactttttc ctctatttca tttccgcgtt gcttcaacaa cgcatagacc
    23881 gctaatgcac tgtttaacgg tactgttcca tcactcaagt ctgtttcatc tagttgacca
    23941 gcaatcaaga aaaatcgagt ggttatagga atattaccga tcatttgctg gtaatcttga
    24001 tagcggttag aaacgacagc aggccccttt gctaactcat ctgtcagaga ctgcgcacta
    24061 tcatcgacaa aatcattaaa aggcgcgcca attgctgtta atgtctgaac ttgtggcaat
    24121 gacgtatcct gtccatacgt tcctaaatag cgtagtgcac taactcctcc catggaatgc
    24181 cccactatgt tgactttatc tatgccgtag ttttctttca gatacaaaag acaagttttg
    24241 atccattctg cttgattcca ctcgttattt ttattatctt caaacaatac ttggaccatc
    24301 ggattgtctg cttttccaga caaatctcct gttgcttgta tttgtccatc tgttcctact
    24361 gttaaaacca attcttttac tgcttgctga tttttttcca aacgccgcaa catcccttta
    24421 aaggaatttt ctgtcccgct gtagccgtgg aaaaaaagtg ttggaatagt ggttttttct
    24481 atcactttac tggattctgc cgttttttct ttatcagaaa cttcagtagt atttatcttc
    24541 gtcgtctggc aaccacttaa cgcaaacaaa ctgataagga ctaaagctgc tgcagttgca
    24601 cgtttgcata aatttttgat tggttctgtc atacacctct tcctttccat atagaaaaaa
    24661 gaaagacttg aaaggccaat catatgatcg acatttcaag tctctttatt aatcgatttt
    24721 atctacttga tcgaaatcaa gttctgtact ggtttcacgt ccaaacatat cgatgttgac
    24781 tttcaatttt tctttttctc cgtcaacttc tgttacttgt ccttctaatc cagcaaatgc
    24841 gccttcgatg attttcacga cttcgccaac ttctacttct agatcatttt gtcgcaggct
    24901 cataccgata gaacgcaaga tatgattgac ttcttctgga agtaatggtg ctggtttgct
    24961 tccggctccg tgtgaaccaa cgaatcctgt cacaccaggt gtgttgcgga caacatacca
    25021 tgaagcgtct gtcatgatca tttcaacaag tacgtatcct gggaatgttt tatgaacgat
    25081 ttctttttct ttcccatttt tgacttcagt ttctttttct tcaggaacaa cgacacggaa
    25141 aataaagtcg gccattttca tactttgcgc acgagattcg atatttgctt ttactttgtt
    25201 ttcatatcct gaatatgtat gcaagacata ccagtttttt tcaaaagatt ccattgaagg
    25261 ctcctcattt cattgtaaaa taaaaaaacc tcagtacacc cgaagttttt ctttcgcttc
    25321 attatagcac taaaagtacg ttgtggctag cgagttattt taaaatccag ccaaataagc
    25381 tttggatggc tgtatccatt acgtaaaaca tcaatccgaa aataatagaa gtttcgatga
    25441 ctactaacgt atctttacgg agttgttttc tgcttggcca agttacttgt ttgatttcat
    25501 cttttacgct ttttagaaat ttcatgagac aacctccctt gctactgttt atttcgtttc
    25561 tttatgtaat gtatattgat tacaatattt acaaaactta ttgatttcca aacgttcgcc
    25621 gctttttcct tcactgactg attttgtgta gttgcgagac ccacaaacgg aacaagcgag
    25681 tgctgctttt ttctttgcca tttacttctg cccccttgct gagaatcaga cttatacttc
    25741 ataacagtat cacgtaactt tttacttgtc aatttgtgaa ataaactatc tattgttttg
    25801 gataaaatta tgatatgatt aatttgaaat taagctaaaa tgagacaatt ggaggtatag
    25861 cgatgctatt aaaaacactt gtatataaga aaaaagatct gacaaccgtt acagagacta
    25921 gtacattaga agaagcatta aaaattttag aaacttccgg ataccgttgt gtgccgatct
    25981 tagattcttc aggtaatatt ttccgtggaa acatttacaa aatgcatatc tatcgtcata
    26041 aagcaaatgg cggtgatatg acgcttcctg tcactcatct tttgaaaaac gctacaaagt
    26101 tcatttacct ggatacttct ttcttcaaag tattctttac gatcaaagag cttccttata
    26161 ttgctgtcct tgacagtgaa aatcattttt atggtatttt gacacatagt acgttgctta
    26221 atatgctctc tcaatcttgg agcgtagaac aaggaagcta tgtactgaca attgcttcca
    26281 caggaaagca aggagatttg gctgctattg caaaaatcat ttcaaaacac agcagcattg
    26341 ccagctgtat tactcttgat atcgaaaaag acgaatacat tcgacgtaca ttgatcacgc
    26401 taccggctaa cactgaagcg aaagtatgtg aaacgatcgt caaccactta gagaaaaaga
    26461 attataaagt ggtagaaatt gaaaatttgc aaaaccaaga agcataatca aaaagcgaaa
    26521 aaagaaaaca gctaatctgc tttctttttt cgctttttat aattagacac gcttttcaaa
    26581 atcaagcgcg cgatctaaat aatacatcaa tggtgctgaa acagccatga cgataaactc
    26641 acttaatgca agcgtgccat aggttgtcca aaatgcttgg ccacctgttg gaactaacat
    26701 ataagcaatc agtcccatgc tgatggtaaa gaagatcgtg ttcaatacta aacgcatctt
    26761 tacatttttc actttgtttt gcaataaagc tgtcaggcct aaagaaagca atgtttggcc
    26821 gccaccaaaa accacatcca tgataccaaa accaaagaag gtattataga cgatgacccc
    26881 gcctaaaact ccccaaagca atttacggtt aaagacaact aaatgattca aactttctga
    26941 aatacgaaac tggatcggcc ctgaggatac aggcgcaact aaaatagtta acgctagata
    27001 aagagccatg atgatgccat ttacaacgat tacggataac cgttgtctgc ttttcgtttt
    27061 ttgcattttc tctcctcttt tctcctagtt tttttaacgc gggatggttg atgaaccgcg
    27121 gttatgtgaa acaaacaata attataacag acttttcatt tatttcaatc cgtcttatct
    27181 gcttttaaaa aagcaaaagc ttaaacaatg taaacgtttg ctaaaaatcg tgggatatgg
    27241 tataaagtaa gtgtaagggg tcataagaat tcccagtaaa gcagacgaag taggatctgc
    27301 aagaacagaa ttgttgtgaa acttaaataa ataaaaaagg atgtgggaat ttgagcaacc
    27361 tcttactaga gaacggtcta gcaagcagct gaagatctaa gtggacatgc cgaaccttgg
    27421 aacttctcct tacgtaagta atggatcatg cgcttgctag gcttttttgt gtttttcggt
    27481 cttcggacct ttttcatttt cctagacttc tgctataata accaagaatt aattttgtaa
    27541 cagatttttt tgaaaaaaca agtttctttc attcaaaaaa tagaaaaagc ttgctaatta
    27601 ttggttaacc agcaaacagg agtattaaaa tatcttgttt tattgaatag caggaggaaa
    27661 tcatgaaatt ccattatcat atattgatca atccatcggc aggcagcgga aatggctata
    27721 aagtagcaga aagaatttta ccggtattaa aaaataagca tattgactat acacttcatt
    27781 atagtgaata caaaggacac gatgctgaga ttgctgaaac tttggcaaaa gaaacattga
    27841 ttccttggat agaagaagaa cagaaaacaa tcgatacttt cccccttttg atcatcgtag
    27901 gaggtgacgg caccttacat caggtacttg atactttcta tcagatggaa gtagaattcc
    27961 cagttgctta tattccagca ggttctggaa atgattttgc tcgaggggca ggccttccaa
    28021 aaaatcccaa aaaaggactt cagctgattt tagcagctca atcaccagaa aaagtccaca
    28081 taatggctta tgaagaaaaa ataagtgaaa aaaaaggaat agctgttaat aatttcggta
    28141 taggattaga tgcagctatc gtccatgcga caaatcattc ttccacaaaa aagcgattga
    28201 acaaatacaa cttaggatca ttttcgtatt tattctctat tctccgtgct ttattcactc
    28261 aaaaaggatt tccaattctg gtagatgcag gaggaaaaca attaagtttt tcaaacgcat
    28321 ttttatgtac agcaacgaag catccttttt ttggcggagg aatcgcgatt gttccaactg
    28381 ctgatgcgtc taaaccagtc attgattttg tcgtggtcga acgaatcaat ttctttaaaa
    28441 tcttgtggct cattctttta cttatacaga aaaaacaatt gaaatcgaaa tatttccatc
    28501 actacacaac aaacagatta cgtatcgttt caacaacacc gcaacatggt caagaagatg
    28561 gagaagaaat ggaaaaacga ccttttgata tcacaataac cacaactaaa cagttgtttt
    28621 ggtgttaata aaagaggttt tgtcagaagc tacatctgga acaccgttta ttcggaaaaa
    28681 aggactgtga cacgactttt gtcacagtcc tttttttact atgcagttaa ttctttgccc
    28741 aattgggttg ccatcgatag tttctttctt tacccaaaat aaattggatc attgaaaaca
    28801 ccgcagcaaa agcaactaag acacaaccat tgaaccaaaa agattctgca aatgtttgat
    28861 tgtaactgag tgtttcactg atcaagttat tattttgaac aaaccaatat gttggtgtga
    28921 atgccgctat tttgctaacc atttctggca agtattctgc tggtacaaac accccaccga
    28981 taaagcaact gcctgtgata aaaatatctt tgatcccacc gctggtttct gagtttttta
    29041 ccaatgaagt gatacaagcc gaaaagctaa tgatcggcaa taaaaacaag aaagtattta
    29101 aaatatgata ccataccgtt atatcccacg aggtgtggat cactaacaga accattagaa
    29161 gtaagaagcc actgaacaac agcagcgagt aactaatcaa actaaaggtt actttgcgat
    29221 tcaagtttcg tttggtaatg ggcgcactct ggtttcgttt actgatctct ttgcgattaa
    29281 acgctagatt gatgacgctg aaaccgctaa aaatggatag aaacaaccca taagacaaga
    29341 aattaaaggt tgtgcccgtc aacagctttt tcatccgctg actatatccc gcacttagct
    29401 caacttctcc agtaactgag agtgccattt gtgtctgttc catagctgca tcgattgttt
    29461 tatcttcttc ttgaaacaga atcagtgttt gcaaaaaggt gttcacttgc tgtgtaacaa
    29521 ctgttttaga aaaggcatct gggcgactct gtatcttgat cgtcggcatc tttcctactt
    29581 cgacttgttc ggtaaagtct gcaggcagat gtaagataat gtttacacga ttaaaataaa
    29641 gagcatcatc gatttctttt tgtgaggttc catccaatgt aacgactgtt tccttgtcat
    29701 tcaaatattt acgaaggcca tcgactacag gcgactcttc ttcactaatc aaggcgattt
    29761 tcgcatcttg cagagaggta ttttcaacat caatattttg cgcaaaaaag aatgtggcaa
    29821 caacagtgat aattacacct atcagtaaat tcgatttatt tgctttaagc aatgtccaaa
    29881 tcgttttata aagcgtcata acgcacccct ctttccatcc aagcatttcc taacgcaaaa
    29941 aggaaaataa atccgatcaa ccatagaaga ttggtataaa aactagccaa atcagtgtag
    30001 aaataaagtt tatacaaact ttcgctgatc aagttgacga gatttaattg accaacaatc
    30061 ggaatatgaa gatcaatcta atacttcact tgttccgacc ccatcatacc agcaaaaaag
    30121 ctcatcgcaa tcgtcactga aatcaagatg ctatcctttt gcaccagatt catttttggt
    30181 atcaaattac caaccaatgt acctaacaaa atcgcacaca acgaccccag agcggtaaca
    30241 atcatcaacc attgccaacg ttcaccaaag tctacttgat agataaaacg aaagacagct
    30301 aacataatat aaatctctgt caaaacaatc gtaaagcttg ctgccaaatt agcgattgaa
    30361 actaacgatt gctttctagg aatcaaggac aagcgaatac catttgacga ttgatttgct
    30421 tgctgatcct gtacattgcg gaccccccac atcgtcccgt agagaatcgc cattccaatc
    30481 agagtgaaga aataaaaact tttgaaactc atattacgac ctgcatgaac ttcttgaaaa
    30541 gatacttctt gttcaaaaac agtcatgatg ggcgcgagat caacgttctc cacttctgcc
    30601 actttactcg aaattatacc aatacgctgt aaaaattgat ccatgacgtt tttcatcaac
    30661 gtctgactca ttcctgcttg acccacatgg agcgaaacag atgaaccatc aatgagataa
    30721 aaaccagcca aatcatctgc atttagcttt tcattcgctt ctacttccga tagttcctgt
    30781 acttcaaata atggtttatc attgacttcg acttgttcta agactgtggt aaaattttcc
    30841 gaaagcgctg tttgatccgc tttgattaca attccagtat taatcgtttc gagattgcca
    30901 ccttgatcaa tctcgccaaa ggccgccata aacatcaatc ctaaaagaat tggaaaggcc
    30961 aatgtccaaa acaacatgga tttatttcgc agcagtattt ttgtgcgata tttataaaaa
    31021 tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc ttgccagtaa tctctaaaaa
    31081 gacatcattc aaggacgctt ctttggttgc tatttttccg taactgatct tttcttcttg
    31141 taagattgta agaatcggca acaatactgt atttagttca gtagttgtta cttggagctg
    31201 ccctttttta taagcgattc catcagaagc tattgttcgc tgaatcgctg acaacacgtt
    31261 ttctgggagt aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca tatcttttag
    31321 agattctttg gttcctttag caatcacctg tccttgatcc atgatcacga tctggtcgca
    31381 aagaatttct acttcttcca tgtaatgtgt tgtatagaca atggttgctc cttgtcgatt
    31441 cagctccttg atactttcta aaattttatt ccgactttgg gggtctacag ctactgtcgg
    31501 ctcatctaaa aaaattaacc gcggcttgtg ggcaatccca catgcgatgt tcaaccgacg
    31561 tttcaagcca ccactaagtt ctcctggacg aaacgaaaca tatttttcta atcccactaa
    31621 ttgaatcacc tcttcaatca aggctttcct tttctctttt tcggaaacat ataaaccgca
    31681 aaaataatcg atattttctt gcacacttag ttcgtcaaag accgcaacgt cttgaggaac
    31741 gaccccaatc attgccttga cagcataatt ttcaggagtc attgttgtcc cgaacaattt
    31801 aatggttcct ttgtcataat ttaacaaaga aagcatacag ttgatggcag tcgatttccc
    31861 actgccgttt ggtcctaaca gacctaagac ttctccttct tgtacttgca gatcaaaatg
    31921 attcaatgct acaaaatctc catagcgttt gactaattta tcaatttcga taatcatcat
    31981 gaaaaactcc ttccaaattt cttgttgatc tgactataat aaagtgcaag aaaagaaact
    32041 agtgacgatt ggctaaacca cacatgacat ttgtcacaat gaaggagtct gctatgcata
    32101 aatctcggct actcaacgaa actagctttc tactggttat ctcattgctt ttgctggtaa
    32161 gtcaaggatt cgttgctatg caagttatct atttattgat cgccgtttta ttcactggtg
    32221 tcagttattt actgcctaca aagccaaatt ggattttcca agttattcta tttcttttat
    32281 gtgccttttt acctgatctt gtcctgtttc tgccagcgac gatgcgagtg atcgcccctg
    32341 aagaaagcca ccttgattac ttgtttttgt taggtttttt gttgatttcg acggttggta
    32401 atcatccccc catcaaggtc gcctttgtcg taattttgct gtttttttcc ttttacttac
    32461 gaaaaaaaga tcaacgaacg accgatcttg agaaaaagta cattgtctcc aaagatactc
    32521 attgggaaaa acaacaagag ttatcggaac aaaataaagc attggccctg tcacaagaaa
    32581 cacaattagc cttgcaaatt ttggaagaac ggaaccgaat tgcccgagac atccatgaca
    32641 atgtcggtca tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa atgatcaatc
    32701 aagaaccaaa actaaaagaa ccattagaaa atttaagtgc caccgttcac actgggatgg
    32761 atcggattcg tcaaagtgtc catgacctcc atgagacatc gatctctttt caacactcct
    32821 tagagtttct gatcgctgat tttccttctt ctgttacgat tgaaggaaac ttgtttgaag
    32881 gattgaatga aacgcagcaa aactgcttct taatggtcat caaagaagcc ttaaccaaca
    32941 gtatcaaaca tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg ccggcctttt
    33001 atcgcttgca aatcacgaat gatggcacca gcccacaaaa aaattctgct cccggtattg
    33061 gtttgaaaac gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat attttccaag
    33121 aaaaccagca atttcagtta acgatcattt taccaaggga gggacaccaa tgataacagt
    33181 agtcattatt gatgatgatc catttgtgac gaaatcgtta caaaccattt tagaaagcac
    33241 gaaagagatc cgtgtgttag gcattggtca ttgtgccaag gatgcgctag tcttatacga
    33301 aacccatcgt cccgatgttt tgctgacgga catccgcatg cctaaacaaa cgggcatcga
    33361 tgctgccaaa gaaattttgg ctaaatttcc caaagccatc cttttgcttt tgacgacatt
    33421 caaagatgaa gaatacatcc gcgaagcatt ttccatcggt gtcaaagggt atttaatcaa
    33481 acagaacttg caagcgatta ttccttctgt caaatccgct tataatggac aggccgtatt
    33541 tggtaatgaa atcatcgaga ctttcactca aatgatgaag aataaaccga cgattgatag
    33601 tcatgcttat gcttctttta gtgaacgtga gcttgcaatc atcaaagaag tagctgccgg
    33661 gaaaaacaat aaagaaattg cagatgcctt gtacttgagt gacggcactg ttcgcaatta
    33721 catcagccaa ttactagaaa agctagattt acgggatcgc acgcaattgg ctatctattt
    33781 ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa cctgaatgat gctccttcgc
    33841 atcattcagg tttttttcac atcttctatt caattgtccc gcaaaccatt tcctcacgat
    33901 tgaaagaatc aaggattcgc agggctgcca ggattcgttg gattgttcgg cgtgttaggc
    33961 ggaaaaatag gcgccttgtc taacgttttt tctagttttg attcttcacg atcgtctcgt
    34021 tctacatcag gactttcatt caatttgaat tctttttcca tctcatctaa ctttttaaag
    34081 tcattgccag attcttttgc taatttcgct aatttatctt cggtttcttt agccataaag
    34141 taacgccccc cttttgattt cagcttagca tatcacactg aaaaaacaaa agaaaacgct
    34201 aaccaacgat catttttagc tatttctcat ctttaaaatc aagcattttt tgccggtata
    34261 aaatcgtagg gagaactgtt ttccatacca atcatcggat ctactaactg gttatcgatc
    34321 acttctgctg taagaacgaa atctttagaa acagcaggtt ctacctcttc tttcttttct
    34381 tctccaacaa atgaagaaaa cagatctggt tccactgttt ccattacttg gtttttcgtt
    34441 tcattcgtcc cactgtcatc ttctacaggt agcttttctt ttacaacccg tggctctttt
    34501 gtatagttcc catcttcaaa tggatcattt ccgaccaaag aatcttcata agctgctagt
    34561 ttggtccgga gagtgatatt cattttatca ttatccatga tccttgattt caacgtagtc
    34621 atccggcttg ctgattcatt cgctacacaa gtgacatagg cttgctcttc ccctaacaaa
    34681 atcaacagtt ccttactgcg agtcactgcc gtgtagagca aattacgctg aagcatccgc
    34741 tgatattgat ggaccatggg caaaataacc atcttgaatt cactgccttg tgatttatgg
    34801 attgagcaac aataagaaag agtgatttta ttccattcgt ttcttttata agtgacctca
    34861 tttgcatcaa actggataac taattcatcc actttatctt cagaatcttt tgccaagatg
    34921 atacccgtga tttctcccat atctccattg aatacattca attcgggagt attgactaat
    34981 tggaggactt tgtctcctat ccgataaacg gtgtcgttcc aatgtacttc cttttttcca
    35041 ggtttccctg gattgaatat ctcctgcatc attttattta acgcatcgat ccctgcaggt
    35101 ccccgataca ttggtgctaa tacttggata tcttgtgcac ggaatccctt atttttcgct
    35161 ttttccacaa ttttagagat caagggttca atttgataag catcacttcg gaaataggaa
    35221 cgatcttttt gattctttgt gaaatctttt ggcagacgtc cttctttgat ctcatgggca
    35281 agaggaataa tactagaacc atcaccttgc cgataaatct gattgagttc cattttggga
    35341 atttgttcga tttccaaaag atcatggagt acttgaccag gaccgacaga gggaagctga
    35401 tctttgtctc ctactaagat aacttgcata ttcgtcggaa tcgctttgaa taatgtgtta
    35461 gccagccatg tatcgaccat agacatctca tcaacaatca aaagtccgcc ttctaattct
    35521 ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc ccagaagacg atggatcgta
    35581 ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg ctcggccagt tggtgctgca
    35641 agtagaatag gaaaaatttc ttgggtatat tttgaaggat ccaattccag tccgttcaac
    35701 tctgcaaaaa gattgacgat tccgttgatc acagtagttt tccccgtacc tggtcctcct
    35761 gttaaaataa atagagggga acggattgct tcagcgatag cttgttcttg cgaaggtcca
    35821 tactgtatgc cgaatatttt ttcgatacgc cgaatattct tctgcacttc tttttcagga
    35881 taatggattt cttttttacg actgagaagg cgctggatcg aattaccgat cccccattca
    35941 gaaaagtaca aactgttttc gtaaagatta gtctcttctt gttgtatctt tccttcttca
    36001 acaagttgga taattacagt agcaacctga tcaggctgga tttcaattgg gcgacttgtt
    36061 tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg gtacgtatgt atctcctgtt
    36121 tgaactgcat gctgaaaaat ctgatgcaag atagctgctc gaattcttct cggtgaatct
    36181 gctgcaatac cgatttgttc ggctagatta tctgcacgtt tgaacccgat tccttctatg
    36241 tcttcgacta gctggtacgg attttcttcg atgatatcca aagactcgtt cttataggcc
    36301 tggtagatac tgaaagctaa ctgactaccg aacccgtaac gattcagtcc catgatcact
    36361 tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca tttctctttt tggcttcgtc
    36421 aaaccggaga tttgttttaa taccgaagga tcagcaatga ttttttcgat agcatcttct
    36481 cccagcaatt ctacaatttt ttctgctgtt ttcttcccga ttcctgggaa tttttcgctg
    36541 gaaagatacg ctacgatccc atttgcagtt gttggctttt cttgctcata tctttcgagt
    36601 ttaagctgcc ttccgtattt aggatgatcc actaattctc cataaaaacg ataagtctca
    36661 ttttcttgaa tatcgccgaa acttccggtg actacaatct ctttttcacg gtaatcagct
    36721 gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat tactaggatt ttgaaaaaaa
    36781 atcgcggcaa ctgtaccaac gaaataaggc atttcttctg gctgcatcgg aagccgcctc
    36841 cttcttctat agtaaaggtt ctggtgctgt ttcagttcct aaaaaatctg tttggttcca
    36901 tactttcagt tcatatggca ataatctgca aggctcttca gtctctaaaa tcgtcaaact
    36961 actattgaac aatccaccca tttcacgcaa ctcacttagc tcttttcctg tcatccattg
    37021 aatggctgca gttaaagaag ctccatgtcc tacgaataaa agtgggccat ctttatgttg
    37081 ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt gccgattcaa tcgtttctcc
    37141 gtgaaaagga gaaggatcat acagatctaa atgattacgc aaattcggca gattatccgg
    37201 atatttctgg tacatttcat cgatcgactg accttctaaa gtacccagcc ctagttctct
    37261 tagcttatct gtataaaaaa tctctaccgg acgagcaagc tctttgttga taccttctgc
    37321 tgtatcacgt gctcgctttg ctgtactgga ataaattgct ttaaaaggaa catattttac
    37381 ctttttccca aatgctttga tttcttcgta actttgtggc aaaagcggag aatcgccatt
    37441 gctcccttga aaacgtcttt cctgattcca ttgtgtcttc ccatgtcggg taaaatatag
    37501 ttccatacgc agcaaactcc tgtctttctt ttattttact ctataataaa ctgtctaatc
    37561 cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta gggaatcctt gctggcgaag
    37621 tgcttcatac actatcattg cagctgtatt cgataaattc aatgagcgga catgctcatc
    37681 attcataggg atgcgtaaac acttttcttc gttttcacgc ataaatgttt ctggcaaacc
    37741 ggttgtttct tttccaaata aaaagaagtg ctcttctcca tcagcaaaat tttcgtcact
    37801 atatacttta tgggcaaatt ttgagatcaa atgtagttta cgattaccta aaaatgttaa
    37861 aaaagcttct aaattactgt ggtaagtaat atttacatca ttccaataat ccaaccctgc
    37921 ccgttttaga tgtttgtcat cagttgaaaa acctagtggc tcgattaaat gaagcggagt
    37981 attcgtggct gcacacgtac gggcaatatt tccagtgttt gcagggatta atggttcaaa
    38041 taaaacgata tgattcatca ggatcattcc tctcatattt atgttcgttt ctattttctg
    38101 acaatttgat ttttgcataa aaaaaaacgt atcaactcgg tgtcagtcac tgcgagtcgt
    38161 tacgttagtg aagctcttct cactaacttt tatcagaaca ggaacctgat aaaaaccaat
    38221 gaaaaaacaa cttgttgcta atataacacc ctcaactttt ttttgcaacc tctactcatt
    38281 aaaaactgct agaaaaacgt tttctcttag ttccttttaa taattctatg tcaattgtaa
    38341 tcttcttcaa aggattctta tataagtctg ctttgattcg ctcttctacc tgccattcta
    38401 aaggtgacat ggccaaaaga tctttttgat tttcctctgg tatctcaaat tcataggtta
    38461 cacgctgacg ttcagttaat ttcattgctt cagcaaactt ggctactaca cgttcattgg
    38521 aataactctg tttttcaggt tgattcggat aaaaagcctg acgtagttcc tttaaataat
    38581 tttcttgtgg caccaccttc aacacttctc catcttctga gagtacacgt tggaattctt
    38641 ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa tgtttgatca gcaaacggca
    38701 aattggtcaa atcagctaca caccaaaatg cttcggcaga ttgttctgta gctgcgtaga
    38761 ctccttcttt tgaaatatca aatccaacag cattttgaat ctttttcaat tttgaaagtt
    38821 cattcaagaa gctaccttct ccgcagccaa catctaatag cgagacttct tcaggtaaat
    38881 gctgcgcaat agtctcaata acgggctgat acatgccaga ttggatcatt ctacgccttg
    38941 gaacgaacat tccttgatcg tattctgttt taaccgcatg ggataaaaaa taaagtgttc
    39001 cttttttgga aaggtcaaaa cggtgattat ttttgcatat caagccatta tcagactttt
    39061 ttacttcttc ttggcaaacc ggacagcgga acagctgatg attttctgcc aaaaactggc
    39121 gtcctcggtc aatttttttt aacatagttc cccctcattt ctgttttatc ttaccgttag
    39181 atgctcactt tggcaactgc taaaaatatg atacaatgcc tagaagaaag tgaaagataa
    39241 aggagaagat tagatgatca aagaattttg cgctgaaaac tacacatcta ttcctctagc
    39301 aattgctaga ggagccgatc ggatcgaatt gtgtgacaac ttagcggtcg gtggcacaac
    39361 cccgagtact ggtgtgatcg aagaaacgtt aagttatgct ggggaaaaag atatccccgt
    39421 aatgacgatt attcgtccta gagggggcga cttcgtctat aacgatatcg aattgaaaat
    39481 catgcatact gatttgattg aagcaaagaa attagggacg gatggcgtag tatttggttg
    39541 tcttactcct tcaggctggc tagacgaaga ggccttagaa ctattgatcg aaacagcaga
    39601 agggttgcag atcaccttcc atatggcttt tgacagtatt cctgaagatc ggcaattcga
    39661 agcaatcgat tggctggtag aacatggtgt tgaccgtatc ttgactcatg gcggtcctgc
    39721 tgacaaaact attgtagaaa atttcccacg gcttaaagaa ttgattgcct atacagatgg
    39781 ccgcttaatc attttgcctg gtggtggtgt cactgcagag aatgcagcac atgtagctaa
    39841 agaattgaac gtaagcgaag tacacggaac caaaatcgtg gatcttcagt tataatctta
    39901 taaacaaaaa aacatttgca acaggcataa tgcttattgc aaatgttttt gtttttttac
    39961 ataaaatagc ccataaataa agaagcaata ttgaaataaa tcagtacact cgtcaaatcg
    40021 ctcaaagtcg tgataaaggg accgcttgct actgctggat caaatcctag acgatccatt
    40081 agcatcggga ttaaactacc tgcaagattc gccactgtga ttgcaaatag catagccatc
    40141 cctatgacaa accccaagat aaaattatgt ttccaaaaac ctacgattag aaaaatcgtg
    40201 atccccgtaa cagctcctgt gacaacgccg gtcaagactt cagccaaaac aagtcgaaca
    40261 aatccatgat ctttctcatc agaaatcgca agtcgacgaa cagccacagc tagcgactgg
    40321 gttccagcat taccggcagt tcctgtaatc agcgaaatga agactgctaa aatactcgct
    40381 tcacttacca agtcttcata gtgactgatt aaagaagcgg tcgacatacc taaaaacaac
    40441 agagtgatca gccaaggcaa acgtcgagac gctgccttta ctggattttc attaatttct
    40501 tccacattga ccccggcaag tcctgaataa tcgctagcag cttcatcatc aataacgtcg
    40561 atgatatcat cgactgtgac gattcctaac aaatgatcat cataatcggt aacaggaaga
    40621 gctaagaagt cataatctct aaatgtttgt gccacatctt cttggtcgtc gcctacatga
    40681 actgaaagca ctcgttcgct catcagatca gaaatcattg cgtcgtcatc attcacgatt
    40741 aggtcgcgca gagaaataac ccctactagc tgaccttctt gattaacgac atagatgtaa
    40801 tagattgttt cagctacatc tgcttcgttc ttcagcacgt acatcgcaga gcgaacagtt
    40861 tgattggcta cgatcgaaac aaattcagtt gtcatgatcg cgccagccgt ttcatcctcg
    40921 taatggagaa gttccttgat ttcactggca tcgtctgccg acatcaaact taaatattta
    40981 gcaatttgct ttttatccag cgtattaagc aagtcaacag cattatctgt atacatctca
    41041 gaaagcatat cagctgcata gctaggacgc atttcatcta ggtattcttt catatgttcg
    41101 ttgtcttctt caatcacatc gaacatatcc gctaattctt taggcgacag ataggaatag
    41161 atcagctgcc tgttctcttc tgtaagagat tggtagaatt gcccctgctc atagatatgc
    41221 atctctaaaa aactgtttct gaatgcctga atattatttt cccttaaatc ttgagtcaac
    41281 tgggcaaatc tttcctctaa ttcttgattt tcatccaaac caatctcacc tcgccttatt
    41341 cttcaaaccg tctcacaact gcctccatgt ctgcggctaa aggctgcttg aactccagca
    41401 actcttctgt aaatggatgg taaaaacgca gatcataaca atgaagggct tgtcttttta
    41461 ttcccagatc cattcgcccg ccatacatat catcaccgag cagggcacag ccaattgcgg
    41521 aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag ctggatatca actaacgcga
    41581 tttctgttag tcgcttctct atccagtact cagtctgtgc cgaacgtcct tcatcagaga
    41641 ccattcgctt caatagcgaa gaataatctc gggcgatcgg caaatcgata tgtccatgtg
    41701 ccgataaatc tgaaagcctt ccactgacta aggcctgata ttttttcaca aacttctttt
    41761 cacgaagttg catatctaat tttgcgtgcg caaatccatg ctttgcaaaa agcatcaaac
    41821 cagatgtatc ccgatctagt cgcgtcacca catggattac ttggttttcg taaccttgtt
    41881 ttttataata agcctttaca cgattagcca ttgtcccatt tggatggtat tgggcaggaa
    41941 tagacgaaac gcctgctggt ttatttacta ccagaacgtg ttcatcttca tacacgatat
    42001 cgatcggtgt ttcatccaaa agtacggttt catgttctcc ttcagcagga atcacaatgg
    42061 ttaccttatc atccttagct agagaaaaca agacattttc tacttgatca ttaactttga
    42121 tttgaccgcc ttgaaatttt attttcgcta acagaccctt tgagatccct ttttctttta
    42181 aaaaatattt tacttgctga gaatgatctt tctcataatc ccatgtaaac tgcatatcaa
    42241 tcctcactaa taaaggcatc tttcacacga tgccaaaaat gcatatgtcg ataagaagca
    42301 aaatggatcc gttcttctgc tattcgataa taaattgagc ggatatttgc ttttgcgaca
    42361 ttcaattgat caaccgttac aagataatct tctgtatttt ccaattttat ttctagccat
    42421 tcatcatggg caatcacgat cggagaaccc aaagtacgaa acactcgatt attcagtgaa
    42481 gcaatctctg ccaactgaaa ggcattgata ctaggatgga tcacagcacc gccgacactt
    42541 ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca atccatctcc tctaaaactt
    42601 tcaaacagct cgtttttgat gtatacatcg gctaccatcg tacgattggc ccgtttaatc
    42661 gttgattcat tcagtgctaa aaaatgctgg tccggtgttt catacaaata actgatccga
    42721 acatccagca agggataact aacactttgt tcccggttcg ttcgtaagct gtctactagt
    42781 tcttccagct cgtaatcccg ccaatccgtg taaaacccta aatgacctgt atgcacgccc
    42841 aagaaacgca catcattcag acaatgactg tagtgatgga acgccgacaa taatgtgccg
    42901 tctccgccga ctgagatgac caattctggc tcatgatcat ctcgttgaat ccctgcttgt
    42961 tctaaaagtg cgttcaaacg ttctgtcact tctatagtct ttgcttcttt attatgaacg
    43021 atcgctactt tcatcccctg cctcctctct ttcgctttct ttcttcaaaa gcgttcgata
    43081 gtagacatca tctgattttc ctcttccatg ggagaataac cgctgtgctt cttggatttc
    43141 ttcacggata ctggacatct cttcatccaa ctgataggca gcttctgccg cacgctgaag
    43201 tcgttcactc atttcttctg gaaaaacgcc ttgatattta taattcagcg aatgctcaat
    43261 cgttgcccag aaattcatcg caagagtccg aatctggatt tccgccaaaa ttttcttctc
    43321 accgctgatc agctgtacag gatactcgat caccagatga taagaacggt agccacttgc
    43381 ttttttattt gtgatgtaat ctcgttcttg gagaactttc atgtcttttc ttttacggat
    43441 gatctcaaca acttgatgga tatcttctac aaactgacac atgatcctta acccggcaat
    43501 atcagacatt tcttcttcta aacgtcccat tggaatatgc cgtaattgtg attttgttac
    43561 aatactatcg actggcttga ctctcccagt gacaaattca atcgggacat gtcgattttg
    43621 ttcacggaac tgtttgcgga tcccccgcag tttcactttc aattctgata cagtttgttc
    43681 atatggtgct aaaaatgctt cccaatctcg ttccatagct gatacctcta cttcattaac
    43741 attcattgtt tattctacca tatatgcaaa gttttttcgt actcttgatt tatgcattga
    43801 ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc gatcaaatga cacaaatgct
    43861 tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac gagatattca tcgctcacta
    43921 tcaattaaca gataaagatt tccgtgtaca gactaatatt tatttcgata ccgttgatga
    43981 acagctgaag aaacaaaatt gtggcctacg gatccgtttc gttggtcatc aagctgaata
    44041 tacattaaaa acacctgctg aaaaagggcg tctcgaaaca acggatacct ttataacaga
    44101 agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt tcaggcgcag tttttcaaaa
    44161 actttctgat ttgaatatcg atcctgcttc attaatccaa accggaaagc ttacaacaaa
    44221 acgggctgaa ttccctattg aagaaggact tttagctatt gatgaaagct ggggagacaa
    44281 tctacacgat tttgaactgg aactagaggt aggggatgca tctgtgggaa aaattgcttt
    44341 tataaatttt ttaaaacgtt tttctttacc ttatcgtcct gctaagaaca agatccaacg
    44401 tatgcttgaa gctaaaaatt agaaaccttt cattttcaca aggcacttga gttcattttt
    44461 ttactttttt ctgttaagtt ataggatgtg aaagaatcta gtcactagtt gtatagaatc
    44521 tattttgggg gagattcata tgatcgaaat ctatttattt gttaatcctt taggggaata
    44581 ctgtttcgaa atggaacaac agttattaca atttatcgaa gaagagtacg ggacaccatc
    44641 aaaagaaaag atgcaatttc gctttcttcc tttggtcaat ttgcagacaa ttggggatgt
    44701 catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa cgtaatcact tattctcaat
    44761 gacttattct gcagcattgg actgcaaagc ggcgcagttt caaggaaaga aaaaaggccg
    44821 ccgttttttg atcaaattgc aagaagcagt cggctgtaat gacataccat attctcaaga
    44881 acttgctgaa tccatatttg aagaaatcgg cggcgatttg aatatgttta aagaagatcg
    44941 tcagtctgac ttcgttaaag aagtattctt ttctgatcaa aaaattgctc gggaaatggg
    45001 catcacaaaa cacccttctg cagtcgttta caattatgcc tgcgaccgtg attttggtgt
    45061 tttagtagaa gtaaaaggta caagtagcct ttggagaatc acagagctgt gtaaaacaac
    45121 agataactca tatcaagttt ttaatggtga acatttacat acaaatgatt ctcagttcca
    45181 ttcaagaact agccatttac acttgctttc taattaaaaa aatacgtcga ctcccttaca
    45241 agagagccga cgtatttttt tcgtttctat tcgagaaaat caaaaataga aagccttata
    45301 aacgatcgat caattcttct aattcattta gacgttgttc aaacatcgac attgcatctt
    45361 caatatatgc agcctgtgtc atatcaactc ctgctttttt cattacttcc acaggataat
    45421 cgctgttacc tgcttttagg taagccaagt agttctctag tgcctctggt tcctggttta
    45481 gtattttctt cgccagtgct gaagcagcag aaaagccagt cgagtattgg aaaacatagt
    45541 aattataata aaaatgcggt atccgtgacc attcgaattt aatttctggg tcttcttcga
    45601 ctgctggacc ataatatttt gcattcaatt taccataact atcacttagg tattcactag
    45661 tcaatggcac acctttttca tcttccgtat gcatgaaatg ctcgaattca gcgaattgtg
    45721 tctggcggaa aactgttcct ttaaacccat ccaagtaatg gttgagtaca taagcccgta
    45781 cacgagggtc tttttctgtt tccaataaat actccgttag gatattttca ttcgttgtcg
    45841 aagcaatttc tgctaaaaag atggagtagt cgccgtacac ataaggctgg ttcgaacgag
    45901 tgaaataact atgaacacta tgtcccattt cgtggacaag cgtaaatagc tgatccagcg
    45961 tatcatgcca attcaataaa atatatggat ttgtgtcata gcttcccgaa gaataagcac
    46021 cgcttcgttt ccctttattt tcgacaacat cgatccaacg ttcagagaat gctttttcta
    46081 cgatggccat gtattcttca cccattggtt tcagtgcttc taaagctttt tcttttgctt
    46141 cttcgtacgt aaaggtaatt ggagcttccc ctaagaccga tgtataaaga tcatacatgt
    46201 gcagtttttc cacttctaat aaacgtttcc gtaattccat gtatcgatgt aacaaaggca
    46261 aatgcttgtt taccacgtct accaaagtat cgtatacact ttcaggaata tgattattgc
    46321 tcaaagacgc ttctctggca gagctgtaat tacggacttt cgctttaaaa ttatgtcctt
    46381 ttatatgtgt gcctaaagta gaagcaaatg tatttctaaa ttgttcgtaa acactgtaca
    46441 atcccttaaa tgctgcttcg cgcacccttc gatccgtgct ttctagcaac tgaccataca
    46501 cgccatgaga taattggact atttcaccat tttctccttc aatcgttgga aaaactagat
    46561 ctgcattatt caaaacagca aatgtatcac ttgaagcatc aaagatttca cctgctccag
    46621 caagaagaga ttcctgctca gcagataaga catgggctcg attatctact atttgctgga
    46681 tataatggcg atagacttcc aattttggtt cttctttgaa atattgccaa atctggtcat
    46741 ctgacaattg caatatttct ggttcaaacc aggaaacggc ttcactaact ttcgaaaaca
    46801 gactgcttgc tcttgcataa agcgcttggt aatctgtatt tccagtgtct tgatcgtttt
    46861 taaggtgcgc atatacataa atgacttcag tttggcgata aaccctcaat acgaattcaa
    46921 tcgcatttaa aaattgagaa gcgccttgat caagtgttcc tttgtgtttt tcagattgtt
    46981 ttaactcttc tgataattcc aaatatttct catcaaactc ttggtcgctt gaaaagatct
    47041 tggtcaagtc ccaagttaaa ttttcaggca attcttctcg ttttggcaac tgctttactt
    47101 ccatgaaatc cactcctttt cttcccttag ctctatctat caaaatagat catagtctaa
    47161 catccacatg tcacagcatc taacttttct actcattcat gtgtctgctt ccatcaattt
    47221 tagcataact aaatcttgtt ttacatagaa aaccgattcc tagcctattt ttatacttac
    47281 tattttttca aagaagacaa gtgtatgcat tcgttaaaaa actgctgata gaccatctct
    47341 tcatcaatca taggaaaaag ccattccctt ttatgttctt ttatttggca aagccatgag
    47401 ttgaaatcag gaactttctg gttttccaag gcttcttcgt acaacattcg ataaagaaaa
    47461 acctgttctt gtaaataaaa gaaaaaccga cttttttgat agatccacga tgatagatgc
    47521 aaaagatgtt tatgacgcaa gtagcattgt tcttgtatgg atactgtatt cttttgcttg
    47581 tgaataagtt ggcgtgtcag ccatttcttc ccatcttcac tcactttttc tatttttttc
    47641 atttggttca ttttaatttc gttactagtg aatatttctt taaggtccct actagaaaaa
    47701 gtccagcagc tactgcaaaa ggaaatatgc cctgatacag tctctgtaat atgatgatac
    47761 aaataaagct tttgtttctt gagatcagct ttccagcaat gcactcctct ttttctatta
    47821 tagtagcagc aacttttttc tatcaatgag aattgcgatt gtccaagaaa agaattacca
    47881 agtatccacc atacttggta actgttattc aaataatttt gggttctttc aataaatctc
    47941 tggtgagaca gtctagaaca ttggatttct attgctagat tcgccaacaa aatatccggc
    48001 ctttgccgca acctcggcaa ataggcttcc aaaaatactg gttctactga ctgtcccagc
    48061 caatccatga agcattcctt taactgtaag tgctcttttg attctctttc actaaagacc
    48121 gaacagttat ctgatttttg atgggcaaaa tgagcacatt ttttctttcc ctttttcaaa
    48181 atcactcttt ttcgacagcc acgacaataa tatttctttc ctttttctgc atgtttagca
    48241 aaacagcact tattatattc attcattgca agcagcattt ttctccctct gaacttattt
    48301 acgtctaaga atttatttac acgaaaaaag agggcgtgaa aaagctattc tgcatcaagt
    48361 aataagaaca gctaaacaaa aatagttccc aatatttttc gtttggttgg gcttgttacc
    48421 gcagatgcaa gcttttgaac acatttatac gaaaaaaaga ctgcgacaca atttttgtca
    48481 cagtctctta ctataatgct gatttataca aaataaaatt tcgttcgctc gatagcatta
    48541 cgcgccatca gacattgtcc atattcattc aatgtttctg gtgtacgatc gctcttttca
    48601 gcaaactcca aaattcgagc aatctgattc tctacatccg cttggttgag attttccatc
    48661 cagaagtgaa cgactaggta atagcgctct tcgaataaat acaaatcagt ccaagcagac
    48721 tctagttcga catctgttgc aagttggatc acttgttcaa aatcttcaaa agcaaaagtt
    48781 gcttgtcttt cctgttcttc tagggcgctt ccagataatt cttcctccgg atcgctgcca
    48841 ataatctgct gacgaagaaa gtcactcaca tcttctgaat caacagtaga atcttctgat
    48901 tgagaaattt cttcatccgg aagattttta ctgataaaca gttctaatcc gtctcctttt
    48961 ggcaaaactt ggaaagtgac agcttcgctt cctcggaact cctcttcgat atcaacttct
    49021 tccaaaatac tataaaagaa attttcgatt tctttgtgat tgccaagtaa atccaaaaat
    49081 gaaacgcccc gttcctctaa atcttcatga ccaattagta cacggatcgt attttcatta
    49141 atatgttcca tttccatttt gctacacctc actttgccac aatgtttaat atagattcat
    49201 tgtagcgaaa tcagcttaaa tgtaaagaaa aaaaatctcg ctgttaagcg aatttaataa
    49261 aaccagcgga tttttcaacc tttcaagcta tcttatttaa ctataacatt tttggcagac
    49321 gaatactata tagccgactt ttcagctaat tttccctctc tatttttcgt gcaatcatca
    49381 tcttctacta cctttttata catctaatca agaatacttt ttgtaaaaac aaaaaaactt
    49441 ggaaaaacta tctttgtaat agtcttttcc aagtttcgta ttatccttct ataatccagc
    49501 catcaattgt gcttgacgta attctaattg tcgtacatcg cgcggtagga accgacgaat
    49561 ttcatcttca ttaaaaccaa cttgcagacg tttatcatca atcatgattg gacggcgaag
    49621 caatccagga ttgttttgca caagttccaa taattcttgt aacggcagat catctaaatc
    49681 catgttcagt ttttggaata ctttggaacg tgtcgagatg atttcttctg ttccgtcttc
    49741 tgtcatttgt aaaatcgctt ttaattcaga actgttcaat ggttctgaaa aaatattacg
    49801 ttcaacaaat ggaatctgat gttcttgcaa ccacgcacga gcctttctgc aggaagtaca
    49861 acttggggaa gtatataatg ttaacaacgt gcaccactcc ttttcgtgat aagaatcact
    49921 tttttgtaac agaagatcat aataatc
//
LOCUS       DAZKCY010000008        48799 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000008 DAZKCY010000000
VERSION     DAZKCY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.008"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(218..1000)
                     /locus_tag="ACG8XC_000413"
     CDS             complement(218..1000)
                     /locus_tag="ACG8XC_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190137.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1065..1901)
                     /locus_tag="ACG8XC_000414"
     CDS             complement(1065..1901)
                     /locus_tag="ACG8XC_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9190138.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1919..3328)
                     /locus_tag="ACG8XC_000415"
     CDS             complement(1919..3328)
                     /locus_tag="ACG8XC_000415"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9190139.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3342..5165)
                     /locus_tag="ACG8XC_000416"
     CDS             complement(3342..5165)
                     /locus_tag="ACG8XC_000416"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9190140.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6008..6334)
                     /locus_tag="ACG8XC_000417"
     CDS             complement(6008..6334)
                     /locus_tag="ACG8XC_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190141.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6380..6742)
                     /locus_tag="ACG8XC_000418"
     CDS             complement(6380..6742)
                     /locus_tag="ACG8XC_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFP9190142.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6779..6994)
                     /locus_tag="ACG8XC_000419"
     CDS             complement(6779..6994)
                     /locus_tag="ACG8XC_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9190143.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7121..7456
                     /locus_tag="ACG8XC_000420"
     CDS             7121..7456
                     /locus_tag="ACG8XC_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9190144.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7532..9529)
                     /locus_tag="ACG8XC_000421"
     CDS             complement(7532..9529)
                     /locus_tag="ACG8XC_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190145.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9627..9902)
                     /locus_tag="ACG8XC_000422"
     CDS             complement(9627..9902)
                     /locus_tag="ACG8XC_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9190146.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10134..10868
                     /locus_tag="ACG8XC_000423"
     CDS             10134..10868
                     /locus_tag="ACG8XC_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9190147.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEKFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10907..12049
                     /locus_tag="ACG8XC_000424"
     CDS             10907..12049
                     /locus_tag="ACG8XC_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9190148.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12144..13535)
                     /gene="sufB"
                     /locus_tag="ACG8XC_000425"
     CDS             complement(12144..13535)
                     /gene="sufB"
                     /locus_tag="ACG8XC_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP9190149.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13540..14010)
                     /gene="sufU"
                     /locus_tag="ACG8XC_000426"
     CDS             complement(13540..14010)
                     /gene="sufU"
                     /locus_tag="ACG8XC_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFP9190150.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(13997..15232)
                     /locus_tag="ACG8XC_000427"
     CDS             complement(13997..15232)
                     /locus_tag="ACG8XC_000427"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP9190151.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15229..16515)
                     /gene="sufD"
                     /locus_tag="ACG8XC_000428"
     CDS             complement(15229..16515)
                     /gene="sufD"
                     /locus_tag="ACG8XC_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP9190152.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVWKELVEVIEGKLNG"
     gene            complement(16528..17301)
                     /gene="sufC"
                     /locus_tag="ACG8XC_000429"
     CDS             complement(16528..17301)
                     /gene="sufC"
                     /locus_tag="ACG8XC_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP9190153.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17498..18346)
                     /locus_tag="ACG8XC_000430"
     CDS             complement(17498..18346)
                     /locus_tag="ACG8XC_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9190154.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18370..19056)
                     /locus_tag="ACG8XC_000431"
     CDS             complement(18370..19056)
                     /locus_tag="ACG8XC_000431"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP9190155.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19049..20083)
                     /locus_tag="ACG8XC_000432"
     CDS             complement(19049..20083)
                     /locus_tag="ACG8XC_000432"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP9190156.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20146..20402)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20478..20816)
                     /locus_tag="ACG8XC_000433"
     CDS             complement(20478..20816)
                     /locus_tag="ACG8XC_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFP9190157.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20818..21174)
                     /locus_tag="ACG8XC_000434"
     CDS             complement(20818..21174)
                     /locus_tag="ACG8XC_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFP9190158.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21180..22367)
                     /locus_tag="ACG8XC_000435"
     CDS             complement(21180..22367)
                     /locus_tag="ACG8XC_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP9190159.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLLHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22558..23403)
                     /locus_tag="ACG8XC_000436"
     CDS             complement(22558..23403)
                     /locus_tag="ACG8XC_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9190160.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23637..24602
                     /locus_tag="ACG8XC_000437"
     CDS             23637..24602
                     /locus_tag="ACG8XC_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFP9190161.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            24979..26022
                     /locus_tag="ACG8XC_000438"
     CDS             24979..26022
                     /locus_tag="ACG8XC_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFP9190162.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26084..26548)
                     /locus_tag="ACG8XC_000439"
     CDS             complement(26084..26548)
                     /locus_tag="ACG8XC_000439"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP9190163.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            26794..27741
                     /locus_tag="ACG8XC_000440"
     CDS             26794..27741
                     /locus_tag="ACG8XC_000440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9190164.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(27952..30024)
                     /locus_tag="ACG8XC_000441"
     CDS             complement(27952..30024)
                     /locus_tag="ACG8XC_000441"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9190165.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(29957..30334)
                     /locus_tag="ACG8XC_000442"
     CDS             complement(29957..30334)
                     /locus_tag="ACG8XC_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFP9190166.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30463..31023)
                     /locus_tag="ACG8XC_000443"
     CDS             complement(30463..31023)
                     /locus_tag="ACG8XC_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9190167.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31173..32465
                     /locus_tag="ACG8XC_000444"
     CDS             31173..32465
                     /locus_tag="ACG8XC_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP9190168.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32525..33643)
                     /locus_tag="ACG8XC_000445"
     CDS             complement(32525..33643)
                     /locus_tag="ACG8XC_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFP9190169.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPNHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33657..35078)
                     /gene="arcD"
                     /locus_tag="ACG8XC_000446"
     CDS             complement(33657..35078)
                     /gene="arcD"
                     /locus_tag="ACG8XC_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFP9190170.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35324..36445)
                     /gene="mnmA"
                     /locus_tag="ACG8XC_000447"
     CDS             complement(35324..36445)
                     /gene="mnmA"
                     /locus_tag="ACG8XC_000447"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP9190171.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLIYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            36849..37448
                     /locus_tag="ACG8XC_000448"
     CDS             36849..37448
                     /locus_tag="ACG8XC_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9190172.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37586..38872)
                     /gene="efmA"
                     /locus_tag="ACG8XC_000449"
     CDS             complement(37586..38872)
                     /gene="efmA"
                     /locus_tag="ACG8XC_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFP9190173.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39143..39487)
                     /locus_tag="ACG8XC_000450"
     CDS             complement(39143..39487)
                     /locus_tag="ACG8XC_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFP9190174.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39624..40769)
                     /locus_tag="ACG8XC_000451"
     CDS             complement(39624..40769)
                     /locus_tag="ACG8XC_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP9190175.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(40949..41923)
                     /locus_tag="ACG8XC_000452"
     CDS             complement(40949..41923)
                     /locus_tag="ACG8XC_000452"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9190176.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(42003..42878)
                     /locus_tag="ACG8XC_000453"
     CDS             complement(42003..42878)
                     /locus_tag="ACG8XC_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFP9190177.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            43008..44915
                     /locus_tag="ACG8XC_000454"
     CDS             43008..44915
                     /locus_tag="ACG8XC_000454"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFP9190178.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(45456..48734)
                     /locus_tag="ACG8XC_000455"
     CDS             complement(45456..48734)
                     /locus_tag="ACG8XC_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFP9190179.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT        13971 a        10279 c         8627 g        15922 t
ORIGIN      
        1 tttttcacta aaatcactac ctgaaacagt ctaaaacact tatatatcaa cgtttaatga
       61 aactatctcc gtttcatttc agatacaaat aaatatagtc gggctccaaa tccgaaaaaa
      121 aataccccca gtagcttatc ctaagctact ggagatgtat aagtacggtc atataacaac
      181 ggggtagggt aaataaatga aaaagatgga agaatcatta ttcaataact tttttaaata
      241 cttcgttgta atcagttacc tgtccgttct taaataccac agaataatct tttttcccat
      301 tttcaagtaa ccgaagtgga atgtctttac caacttgctc tgtcactgca ttactgatac
      361 ctgttagatt agctaataaa tctgataaag attgagcatc tgtagaaatc tctgtatcag
      421 tatttatcgt gattccttta tcttcattta caagcttcca agtaggtggt atagctgtgt
      481 atgtttcgat gaatgaatca attctttcat ctatcgtctt cccagtatct acttctgtct
      541 cattactctt agatgaatcg attctttcat ccatcgtctg ctccgtaact gcttctgtct
      601 cattattctc accggaaact ctcttcacac ccaaacttat atattgtcca tctttagtgt
      661 gcatagttaa aattcccacg cttttactaa taaattcact ataaccagtt ctttcaatag
      721 agacttcaat gtatttcaca ctatcatact caaaagaaaa atagacatgt aaaagcattt
      781 tgttgtttac atctacataa taatcctttg actctacttg agaccttcct atacggaata
      841 cagctttact gtctgatatc tctataccag catccttatt attaatctgc caatctccaa
      901 gtagctcttc tggcaagcta gtagtttctt gtgaacactt taccacttca ctggtagagg
      961 tcgaaacatt cacctccgca ctgttagaag aattttccaa ggaatcagga gccttagcac
     1021 aaccaacaag tctttatata ttgtgcagcc gatattttct tcattcagta cacccttgaa
     1081 atatgcagtg ttagataaag cagttcgtcc tgatttgctg aggcacccat ctcttcttga
     1141 acgtactcct tgattttcaa ggccgcccga tactctttcg gaaaacgctg cttcactgaa
     1201 gccagcaaaa aatcatcctt ctccggcaac tcttccttct taatcattcg ggcaataaaa
     1261 aactgtaaat gcgtcacaaa tcttaagtaa agcatcgaat ctttatcaac ctttttttcg
     1321 ggaaacagat atcctagcat agccaagata tcctttagtg ctcttaccga gagcatggtt
     1381 tcttccatat ttgcttgttc actttgggca tttacaatgt gaaacgcaat gtttccagcc
     1441 tcctcttctg gcaactgaac attcattttt ttgttgatca attggatagc atagcagccg
     1501 atctcaaatt cttttgggta atacttttga atttcaatca gcaggcgatt ttgaaaaaca
     1561 atgccttttt gactgcgttc aatggcatgc ttcaagtgat caaccaaggt aaaaaataga
     1621 cttttattca gcttaaagtc aaacttggcg ttggcataat cgataattcg cttgacgcaa
     1681 ataattagat catccggagc agcctcaaac agtttaatgt aatccttttt tagagcatca
     1741 gaaagtgaat ctaatttaaa tactttttgg attttgtcag ccggaaccaa atcgcctttc
     1801 tttagttgaa atccaatccc tttccccatc aaaattactt ctttgccttg cttatctaag
     1861 caaagaatta aactgttgtt taacgtttta attactctca ttagattcac cgttctctct
     1921 aagctaagcc atttcttgcg attacttttt gataccaaaa gaagctttct ttttttattc
     1981 gttttaattc tttaagattc tcatcggttc ggtcaacata tataaaacca taacgcttat
     2041 tgaagccttg atgggtacta acaacatcaa tcgctgacca aggtaaataa ccaaaaacat
     2101 ctacaccatc tgttaaagct gcttgaatag ccagaatatg tttggctaga taatcaattc
     2161 gatcctggtc attaattcgt ctatcttcct caactatgtc gggtgcccca tagccatttt
     2221 cagtaattaa aataggtaaa tcatagcgat cgtacaattt cctcaatgtt aatcgtagtc
     2281 cagtagggtc aactacccag ccatatggcg ttttatctac ataaggattt tcagctgccc
     2341 ggtagacacc ttcttctccc aacatgatct gttgatcccc cgctctagcg gaaacatctg
     2401 atccgtcatt tttgctggca gcgatggtgg cagttgaata ataattaatc gcaataaaat
     2461 cgggtttacc ggaggataaa attgcctgat cctctgcctc aattttagga tataatccac
     2521 gatcattcaa atagttttca aacaaataat tgtattttcc ccgaactgct acatctaaga
     2581 aagaccagcc tctcaatgtt tcccaattat gggctgcaat tgcatcttct ggactggagg
     2641 tagcttgata catagccgtt aaattgattg ctggaccgat ttttccttta agattcaact
     2701 gatgatacaa gtgaattact ctagcttgag ctaataaaac atggtgatta atttgaaaga
     2761 gttctttttt tgaaggcagc tctccacttg gtggcaaacc aatcgctcca ggatgaagga
     2821 tcatcgtgtt ttgctcatta attgtcagcc aatagttcac cttgtcacca tactcagtaa
     2881 gcaatacctg tacatagtcc acaaaataat cgatagttgc tcgatttaac caaccgccct
     2941 gctcctctag aaaataaggt aaatcgaaat ggtacatggt gacaatcggt tcgattccct
     3001 gattcaggca ttcatcaatt acttgatgat aaaaagaaat tcccttttga ttggttttat
     3061 tattttcaat atccataatt cgcgtccaag cgatggaaaa gcgaaaagct tttaagccta
     3121 attctttaaa cagctgtata tcttctttat agtgatggta aaaatccgag gcaacttcaa
     3181 aattagctac cccttctgta tgctgatact tgtcaataat agacggttgt ttcccatcct
     3241 cctgataggc accctctgcc tggtaggcac taatggaggc accccataaa aacttctctg
     3301 gaaatgctga aagctgtttg tattgcacaa aaatcctctc cttaactaat ttgcatcaat
     3361 ggttcgtgtt cctttaagct tccgcttgct tcactaattc ctgcgtaatt ttcactgttg
     3421 gtcactacca caatcactgt atcatcgtag ccagcgtttc taatggcagc gaaatcggcg
     3481 ttgattaata attgtcctgc tgacacctga tctcctgctt ttacaaatac ttcaaagggt
     3541 tcccctttta aattcacggt gtcgatcccc acatggatca ataattcaat accttcttta
     3601 gaacgtagac caatcgcatg tttagattct aaaatagtaa tgacttctcc ctcaacaggt
     3661 gaataaatgc tgttttcttc tggatgaatg gctacgccct ttcccaatag tcccttagaa
     3721 aagacagcat cgttcacttc agaaatagga cacacggttc cttttaccgg attttcaaca
     3781 atgactgctt cggctattgc cgtttgctct gattcatcag aagtatcgtc aaatccaatt
     3841 agataagcaa tgataaaggt tgcaacaaag gcgattacac acgtaataat tgcgtgaaca
     3901 atattcattg gattttctcc aataaaggct ggtaaggctg ctaagccagg ggaaacaaag
     3961 gcatatctga ctaaaccgct caagccagca tacaaaccag caattgcacc accaatcatg
     4021 gaagcaacta atggtttttt cagcttcaag gttactccat agagtgaagg ctcagtaatt
     4081 cccattaaag cagtgatccc agtggacaca gctagctgct tcaactcttt gtttttagtt
     4141 ttcgcggcta cagcaaaggt agcacctgat tgagcaatat tagaagccag cattcctggc
     4201 ccattgatca tttcagaacc cgtactgctc aattgcatag tagcgattgg cgtcattgcc
     4261 caagctgtac ccgtaaccac caaaaatggc tgcaaaccac ccatcaaaaa tggaattaac
     4321 caagctgctt tgccgttaat ccattcagcc cccattgcta taagatcgtt cagcaacgtt
     4381 ccaaacggtc caatgacaac caacgctaaa ggccccataa ttaaaaatac cagcatcggc
     4441 ttagtaaaga atttaataaa cgaaggcgaa attcgatccg caaagggctc tacataggac
     4501 atgatccaaa cagtaaagat aatcggtaaa actgatcccg cataatctgc agctaatacc
     4561 ggaattccta gaaaagaaac ggcatcgcca gtagaaaaaa gctgtgaaat atttggatga
     4621 agcaaaatgc cagcaacagt aatcgccaaa attgggttga catcaaactt tatggctgcc
     4681 ccataggcta atagtactgg cataaaataa aaggctgcat cagcaatagt attcaaaatg
     4741 atgtaagttt gactactgtc tgatagtaca ccagtcaaag tcaagacggc caaaatggct
     4801 tttaccatcc cggcacctgt tatagctgga atcatcggtg taaaggtggt agaaatgaca
     4861 ctaatcaatc ttgaaataat actttcttga ttgtctgaag aggggccctt tttaattgaa
     4921 gttcccactt ccttttgaat ttccttgaag gctgtttgga cttcattgcc aatcaccact
     4981 tggtattgtc caccgctatc caccacacta attacaccaa gtgttttttc taaggctttc
     5041 gtatcggctt tccttttatc tttgagttca aaacgcagtc tggtggcaca atgggttaag
     5101 ctgaccacat tttcttttcc cccaacctgt tgcaatacat ttcttcctac ttctctgtaa
     5161 tccattattc tctctcctta aagcagtaaa aaagaccaag tcaaaccacg ccccaatcaa
     5221 cattgaagtg ttagtttgtc ttggtcttgc ctgcataatc agtaacatgc caataacgtt
     5281 attttgctgt aatccaataa tagcacatta tgatagcgca atcaacaggg aatttaattt
     5341 tttatctttt cgtttgaccg tatagggtaa tataacagtt agatcacatc aactagggta
     5401 tatctgcgta tacttactgc taaaataagt atatctaata ttaactatac cagtaaaacc
     5461 attcctacat aaccagcggg aaattcccaa aatcgtgaaa gcatcaaaaa tgcatataac
     5521 ggatcacaca acttcaacaa ttctgctctt gggaaaaatt gatgaatttg gtgagtaaag
     5581 gatggaataa acagcattcc aacaatatag gcaacaatag tgatacttgt ttttttggta
     5641 aaaatcagta agcaatagcc aatttcaatc attccgagat ataaaggtat cgatagaatg
     5701 atttgggcta gtattcttaa aaattaacta gcaaatgttt aaactgattt atttctaatc
     5761 tacatgtagt aagtatttca attccaccaa ttatcaataa aaataccaaa catatccata
     5821 ataatttttt ctttttcatt tcttttatat tgttataaca gtgaataaca taaaaattcc
     5881 cgacaatcat tgtaatattt ttttgtcacc tgtattagta tttaatttat aggaaactat
     5941 aaaaactccc tagtgaattt acacctttgg gagttcctgt ttggacggta tgcataatac
     6001 atttaaatca atttaacatt aacacctcag catatggtat ctccacatat atacggtctt
     6061 tctttggata gtactcaata tttatgttga tagcacttcc attgtctaat atagctttag
     6121 cccggtatgt gttatctcca ttttcatgag taatcgtcac atcatcacat gatcgattta
     6181 agttgtactg ttcttgtaaa atctgtgtta ccaatggttt tgatgacttc tctaatccag
     6241 atgtacctgt catttttgaa aaaataatac ctataatagc caagacaaca atacctataa
     6301 tgacaaactt cttgttcata tttactttat tcaacttttt atctcctatc tccaaaataa
     6361 tttgctctta tccctcttat cagtacattg acatccctaa aagtacacca aagattgctc
     6421 cagctacccc tgctatcatc aaaatcgtac ccattttctc atctttttct cgatataagt
     6481 agcccataat aacagcaaca gcaccaaata caacaggaat aaatgctaac gatactacca
     6541 tagaaatcca accaataatc aaatacagct tccctgtctc ttgtccatgt acgatttgtt
     6601 ccttcatttc attaattgtc tgctcagtac ttttctttac tgtgtctgtt ccggttgtct
     6661 catctgtttc ttgtagtgct ccacaaaact ggcaatactt actttcatct gttatttctt
     6721 tgccacattt tctacaatac atgtcttctc ctcctaaaat cattcaattg cttattagtt
     6781 aactgtttac tttattaaat acatcagaaa acagctcatt cattggtaca cttaatatct
     6841 cagaaagcat cttcatttga tagggtttag gaatcgttct ccctgtttgc cacgaactta
     6901 cacattgctg tgtaacattc attttttttg ctaactccgt ttgagaaagt cctttttctt
     6961 ttctgtatcg gctgagatta cttacttttt tcatgattct cccctcctgt tttattatat
     7021 acaacaatat gatgtatttc aactcataaa atataaaaaa cttcaaatac cattacaaat
     7081 acaacaactt gttgtactat actgagatgg gggtgtatta atgaaacaga aacaatatgc
     7141 aaaggtcatt agagaaaagc gaaaagctct cgggtggaca caaaaagaat tagctgaaaa
     7201 aatcttctct acacaacagg cagtagcaag atgggaaaat tctgtaacag agcctaacct
     7261 cgatagctta acagctcttt ctagagccct aggaacccct gtaagccatt ttctagacaa
     7321 tgttgtcgta gattatgagg aagagttttt ggctctctat cgttctctta gcactgaaga
     7381 tgcagttcga acaattgact atatgaagct attaaaaagg caagaaaatg aacgcaatca
     7441 gctattgaaa gattgaaaaa taaataaccc accgaacttt aacggtgggt gtattgagat
     7501 gaaataagat taagagctat ggataacctg tctagtttaa tacaattggg tcttttctct
     7561 tccttactct ccgtccatca tctcttgtta cacttgtaaa ttgatacaga ggaactatcc
     7621 cagctcctga taatatcaat tcttttattg tttcatctat ccctgcccct ttcgctaaat
     7681 atattaaaaa actactactt gctttcacat tgtatagttt cttaatatcc ttagaagctc
     7741 gttcaagagt gaatccttgt ttaacaaagt agcctctgtt ttttattaaa gcatttttca
     7801 cgctgttatc cccaacatta cgtaggcgtt cacaaacagt gttaataagt tctttctctg
     7861 tcattagata tgtcccttgc cacgataagc cctgtaaaac ttcatgtgct tcatctaaaa
     7921 tcaattgttt ttccttgctg agttctgatc gggaattttg aggaaatact cttttagcct
     7981 ctttttcgtt aaaacatatc ttaacccatt cataagaaaa atattgatat gtaaaatgaa
     8041 ttttttccag aagtggaagg atatcttcat tcaatttttg aatcacagaa gttgcattaa
     8101 aaacgtctaa tcgatagaca ttgcttctct tatgattaaa gtttcctaac gctttctctt
     8161 cctttaacct atttaggtat tcgagcatct tcaaaggaac atcttcatca tctaattttt
     8221 ttatcaatcc tataaaagtc ataagtgata gtaacttatt acatttatct atgtcataag
     8281 ttttaccata gtaagaactc atttttttag ctaattgttc ccccgacaaa atacaagtca
     8341 cactttcctt actatcatca aaaaaattaa catcagaaag caagacatca tagacacgtc
     8401 gcatttcctc tagataattc cttattgtcc tgaaaagttt gtctttcatt gattcaagag
     8461 tgtcaaaaag tacttttgat tcctctttca attttcgaaa atgtgtagga tacaagtcta
     8521 ggttctctaa tagccaatta aatgtttcta cccgactgtc tttctttaaa attagcatca
     8581 caactccaat aaaattttca ctgatatcac aattactgga atgacaataa aaaatggaat
     8641 gcccagtttt actggtaaaa atactagcag agggattatg atcgttgtga aacagacatc
     8701 ttaagttttt acttgactta agtaatttat tcatatcaat ggttaacaat ctttcataaa
     8761 tctcattaaa agacatgtcg gaggaatcaa acaggcagtc tttaaaccac tgtttcattt
     8821 cttctttgtc gtcatttttt atcaacttaa caaaatcggt ttcattgact agttcatact
     8881 ctctccctga atcactttga caatttttag aacatgtcat ggtaaaatca atcggaagct
     8941 cagtaatatc taacatgttg tcttcattaa ttattatcac tgcatctgac ttatatcctc
     9001 caaaaaacat tctattcgaa gcatttacat tgtcatcaca cccggggatg ttctttttca
     9061 agtattcgta aacagcaacc attttaccat agtcagtaat tgcttctttt aatagaaaga
     9121 caatcttgta tttttctaga ttttcagagt accgaatact tttttgtatc gcaaacgcag
     9181 aattatgaac taaattgata gataaaacct tttctacgct taagtatcta ctatcatctt
     9241 tactcaaatc tactaccttc cccttctcat ctttaacagt gttatcgaca tccagaataa
     9301 ttactctggt ttccacaaaa ctctccttgt cattgccttt attctttgtc ttgcttggaa
     9361 taaatgattg tgatgaattt agtatctcta caaactcatc tcttgtaact tctctatagt
     9421 tatctaaaca cctatctctt acatcagttt tttctttttt tgtaactggc ttctctaata
     9481 atttttcttg atctacataa attggaatat taatcattga tgtttccatt tgtgtttcct
     9541 ccttataaaa ttctgttaaa gactaaaaca tacagataat aaaaacaaac tctttattac
     9601 tatatgtttt agtcttattt gtttactcaa cctttatacc gttccatgaa actatcaata
     9661 tcagattttt taaatctaat gaccgtatca atttttataa caggtaaccc gtattgagaa
     9721 acatattttc taatcgttcc tactgataca cctagatatt cacttgcctg ctgatagtcc
     9781 atatactcta gagtgactcc agaaataatg tcttctagca taggctttag cactgtaatt
     9841 atctctttca cttgttgttc aacttgtata gaaacaatgc tatcaatgat ttgtagagac
     9901 atatttcccc ctcctttcgt gattgcttga acaaattttc taagaattat cttcgtattt
     9961 tttgctcaaa ataaaagtat tttgttatta accgattaat caaacaaaaa atgattacca
    10021 gttcatcgac atacacataa tacctcgata aaaaaggggt gtcaacatct ttgtaaaatt
    10081 tatttacaac accccttttt tgaggtatac taattttagg aggtgtggtc aatatgataa
    10141 aacgtccacc aataaattat ttagaacgaa aaaagatact cggaacaaaa ataaaagcta
    10201 taagaaagag taaaaaatta acacaaccag catttggtct tatgattaac aacggtcaat
    10261 taattgacaa aaaaactatc tacgaatggg agaagggaac ctatttacca ataccagagc
    10321 gattatcccg aattgctgat ttgggaaata tgagtataga ggagttagtt tgtggcaatg
    10381 tagaagaata tattttggga attattcttt atagagatag cattgtacta gatggtataa
    10441 catttccaga taaaaacctg ttccagcatc ttcgtcaaca gttcccacca gtacactcta
    10501 atttagatac atggctggat agatattcca aattagaacc agagatgcaa gaatttattg
    10561 ctaacaagac ttgtaataaa gttaaaaatg aaaaaattag tcttttcaat atcttaaaaa
    10621 tagaagaatt attcatcaat gctattgtag aaaaatttga caataacatt cttttcctca
    10681 ctagcagtat agaagaacta ctagaaagaa tggtagatga atggctacct attcaattga
    10741 aagacatgtc ttatcccgaa gaagctgtga gagaaattac tgataatata aacaagctag
    10801 aacaaacgat ttcatcaatt gggaaaaaat acactaagaa aaaaatgaag ggaggtgata
    10861 ccatctagtg gtaagtactc cttgtaaact aagaggagat gatttaatgg caaaaatatc
    10921 aaagtacaaa aagaaaaacg gtcaagtggc gtggatgtat aaaggacaca ttgccaccga
    10981 tccacgaact ggtgaaaaaa taaatactac gagaagaggt tttagtagca aacaagaagc
    11041 tcaaaaagat tatgacgagt acagacatca aatattatac ggtgttaaaa agaaagcacc
    11101 tgatatgaca tttgaagacc tgtataatga atggataact catcagcgta ccagtgttaa
    11161 agcttcaaca atcgcaatct cagtaagata cgcaaataat caaatacttc cagcatttgg
    11221 taagcttaaa ctatcgaata tctctgtccc atattgtcaa aaagtggtag atgaatggca
    11281 cagtaaatat gaaagttacg attatatgag aaaacagact gcacaaattt tacgctacgg
    11341 ggttgcaatg caatatattg acaataatcc tatggaaaaa acacttcttc cacgaaaaaa
    11401 agagtatgag aaaaatcgta aattttactc aaaagaggag cttaataatt tactggatgc
    11461 ttttaaagat tttggtaaca tgaaacaata cgccttcttc agattattgg catacacagg
    11521 tatgagaaag agtgaagtac tcgctttaca gtggaaagac atagacacgt ttaataaaga
    11581 actacatgtc aataaaactc ttgctgtaga tgaatttggt aaagttataa ttcaatctcc
    11641 gaaaacaaga gcttctagac gagttatctc tttagatact gagactcttt ctatcctcaa
    11701 caactggaaa ttacagcaaa aagaagagta tttaaaactt ggatacaata ctagctctaa
    11761 agagcaacat gtttttacaa cagttaaaaa cactctatac attccaaaca ctgttaatga
    11821 ttggttaaga tatattttga aaaaatataa cttgcctcgt attacacctc atgggtttag
    11881 acatacacat gctagtttat tattagaagc aggagaatct gtcaaggtag ttcaacaacg
    11941 attagggcat gaaaatagta aagtgacatt agacatctat gcacatatta caaataatgc
    12001 acctaagaaa acaggtcaag actttgctga tatgatggct catcagtaaa agagctggtc
    12061 aaaatctggt catttctagt atacaggcaa aacaaaaaag ctagaaccct tatatatcaa
    12121 gggttctagc tcctataaca ggtttaccca acactgcctt ccatctcata actgatcaat
    12181 cgattgagtt cgactgcata ttccattgga agttctttgg tgaatggttc aacaaagccc
    12241 atgacgatca tttcagttgc ttcagattcg gtcaggcctc ggctcatcag atagtagagt
    12301 tgttcttctg agatttttga gactttggct tcgtgttcta aagagacttg gctgttgtgg
    12361 atctcattga atgggatcgt atcggatttc gaccattcgt ccatgatgat cgtgtcacat
    12421 tcgatatgag agatcgaacc cgcactgttt ttggcaaagg ttacttgtcc tcgatagttt
    12481 acttctccgc cgtcttttga aattgattta gagacgatag agctagatgt atttggtgca
    12541 ttgtggatca tttttgcacc cgtatcttgg atttggtttg ctcctgcaaa ggcgatggag
    12601 agcatggtgc ctcgtgcacc ttttccatct aagtaaacac ttgggtattt catcgttgtt
    12661 tttgcaccta ggtttccgtc gatccattca acggttgcgc cttcgtatgc ttttgctcgt
    12721 ttggttacta agttgtacac attgtctgac cagttttgga tcgttgtgta acggcaatac
    12781 gcatcttttc ttgtaaagat ttctacgatg gcggcatgca ggctgttact tgaataagta
    12841 ggtgccgtac aaccttcgac atagtggatg cttgctcctt cgtcaacaat gatcagcgta
    12901 cgttcgaatt gccccgtgtt ttccgcattg atccggaagt acgtttgtaa tggaacatcg
    12961 actttgacgg cttttggcac gtaaataaag gtcccacctg accatacggc tgaattcagc
    13021 gctgctaatt tgttgtcggt tggcggtaca agttttgaaa agtactcttt gaataattca
    13081 gggtattctt tcaaagcaga gtcagtgtca gtaaagacga ttcctaactt ttcgaattca
    13141 tctttcatat tatggtacac gacttctgat tcatattgtg cggaagcacc ggctagataa
    13201 gctcgttcag cttctggtat accaatcttt tcaaatgtat ctttgatttt ttctggtacg
    13261 tcttcccagt cacgagcggg tttgtcactt ggtttttggt aatatttgat ggcatcgaaa
    13321 tcgatgtctg aaaggtcggg gccccatgtt tgcattggca ttttgttaaa tgtttctaga
    13381 gatttcagac ggaattcaag catccattct ggttcgcctt tgattcttga catctcacgg
    13441 atcacttctt ctgtcaagcc ttctcctgta ctgaaaaccg gttcgacatc atcgtgaaac
    13501 ccgaatttat attcttctaa ttctggtacg cccatgttct cactcctctt ccttgtggta
    13561 atgatggaaa gctgcggtac catcgttttc cattgctttt cctagtgctt tccaggcaag
    13621 tgtcgcacat ttgatccgag cgggaaattt agctacacca ccaagcatag cagcatcgcc
    13681 taattggtcg ggatctttga cttcttttcc ttggacaagc tgtagaaagt ctacagttaa
    13741 atgttctgct tcttctattg attttccgat gacggcgtct gtcatcatcg aggcacttgc
    13801 cgtactgatg gagcagccac tgccgtcaaa ggcgatattt ttgatgattc cgtcttttac
    13861 atcgacttgc aattcgatca catctccaca tgtggggttg ttcaattcga tcttgcttgt
    13921 cgagtcagtc aagcttccac gatgatgcgg atgggaagaa tgatccaaga tcacttgacg
    13981 gtataaattg tctaatttag atagtcccat gttggaaaaa ctcctttgtt gccatgattg
    14041 cttcaactaa acggtcggcg tcttgcgttg tgttgtagaa ataaaagctc gcgcgagcag
    14101 tcgctgatac ttcaaggtac ttcaatagcg gttgcgcaca atgatgacct gctctgacgg
    14161 ctaccccttc catgtctaaa gctgtcgcga catcatgggg atgtaaccca tccaaattga
    14221 atgcaacgac tcctgtccga aaattcgggt cttgtggacc ataaacagtc aatccatcaa
    14281 ttgcttgtaa ttttggcaac acataagcgg ctagttcttc ttcatatcga tggatattgt
    14341 ccataccgag tttttgcaaa taatcgattg catggcctaa tgcgatagcg ccggcgatat
    14401 ttggtgtccc tgcttcgaat ttccaaggta gttctttcca tgtactgtcg tataattcga
    14461 caaaatcgat catttcgcca ccaaattcaa caggttccat ttcttctaac cattttcgtt
    14521 taccatataa aacaccaata cctgttggtc cgcacatttt gtgtccgctg aaagcaaaga
    14581 aatcacagtc tagtgcttgg acatcaatgg ccatgtgcgg ggcagattga gcaccatcta
    14641 ctacgatgac cccgccgttt tcatggacaa gttcagctaa ttcttgaaca ggattgaaga
    14701 cacctaagac attggataca tgagcaatgg agacgatttt ggttttgtcc gtaatctttt
    14761 tcgctgcatc ctccatatcc aggaagcctt cttctgttag ttcgatatat ttcaagacag
    14821 cacctgttcg ttttgccaac tgctgccacg ggataacatt agaatgatgt tccatgtaag
    14881 agagcactat ctcgtctcct gctttgatat agcggtcgcc gaaggttctg gctacccaat
    14941 tcaagcttgt cgtcgtacca cgagtaaaca gcacttctgc tgtttctttt gcatggatga
    15001 acgcccgtac tttttctctt gcttgttcat aatcatgggt cgcgcgttct gctaacgtat
    15061 ggacaccgcg atgaacattg gcgttatcgt gttcgtaata gtacgctaat gcctggagta
    15121 cttgttttgg cttttgggtc gtcgctgcat tatccagata aactaaaggt tcatcattga
    15181 cgtcttgaaa taagatagga aaatcattgc gtagcttttt agcatcgatc atccattcaa
    15241 cttcccttcg attacttcga ctaattcttt ccaaacttgt tctacaggga ttgctgttaa
    15301 gacagaaccg aggaatcctc gaatcactaa gcgctctgct tcttctttat gcaatcctct
    15361 actcatcaga tagtacattt cttctggatc aactcgtcct acactggcag catgtccagc
    15421 agttacttcg ttttcatcga tcaaaagaat aggattcgca tcaccacgtg ctttatcaga
    15481 aagcatcaag acacgacttt cttgttgggc atctgcacct tttgcccctt tcaagatgtg
    15541 gccgatacca ttaaaggtca atgttccttt ttctcggatc actccgtgtt gcaaaatatg
    15601 cccgatcgta tgaggggctt tattggttac tcgtgtatcg attccttgga tttgtctgcc
    15661 cgagctgatt gccacgattt tgacttctgc gtgtgcacct tctcctgcta aatctgagtc
    15721 aaagtcagcg atcacatgac cgtcattcat gacaccaatt gcccagtcga cagaagcatc
    15781 tcgtaaaatg tgcccacggc gattcatata agaagtaata ttttctccta attgatctac
    15841 ggctgaatat ttgatttttg aaccagcttt agcgatgact tccacgatga tattacctga
    15901 tgattttgct acttgttctt tggttgtctg gaaacgttcc agataagaaa attcactatg
    15961 ttcatctgcc acgatcaaca catgcttgaa gaagtgctca tcggatgcgc catcttgaat
    16021 gaataaggac tcgagtggtt catcgatcac gacattttta ggcacataca ggaaaatacc
    16081 gctgttcata aaagctacat ggagtgcagt caattggtct tcgttcatgt ttactgcttt
    16141 tttcatataa tattcttgga ctagttctgg atattcgatc attgcagtga acaagtcggt
    16201 aaaaatgact cctttttctg ctaattccac aggcagttgt tcaaaaatcg tccatgaacc
    16261 ttgttggaca agcacaggat tgtctttcat tgcatcaaat gccggaatcg tgcctgtttc
    16321 actgatggtt tgtgtttctt tcacatcaaa taacgaccag cgatggaatt tcacccgatc
    16381 gatatgcggc aatggcagtt catctgcttt cgctagtgcg tcttgacgga aggttctcat
    16441 ccaatcaggt tcgcctttac ttaaagaaaa atcatttacc gcgtcaagat aatcgagcca
    16501 gtttttttct ttcattcttg ctccccctta cgcttcttct tctttatagt cgattcctaa
    16561 ttctttactg ataccagcgt agccttctgc ttccagacgt ttagctaggt cagcattccc
    16621 tgtcatgact actcggcctt ccatcatgat gtgaacgaca tcaggtgtga tatagttcaa
    16681 taatcgttga tagtgggtga tgatcaatgc accgaaattg tctccgcgca tttcatttac
    16741 tcctttagca acgactttca aagcatcgat atccagacct gaatcgattt cgtctaaaat
    16801 tgcaaatgta ggttctaaca tcaacaattg caggatctca ttccgtttct tttctccgcc
    16861 agaaaaacct tcgttcagat aacgttcagc catttcttct ggcatattta gtaaagccat
    16921 tttttcatct aactttttca aaaatgccat aacagaaatt ttgtcgtttt catcacgttt
    16981 cgcattgatc gctgcacgca tgaattccgc attcgtgata cctggtatct cacttggata
    17041 ttgcatagct aaaaataagc caagtcgcgc acgttcatct acttccaagt ctaaaacatt
    17101 tttcccgtca aacaaaattt cgccttctgt tacttcatag tttggattgc ccatgatagc
    17161 ggcagataag gtagattttc ctgtgccgtt aggtcccatg atggcatgga tttctccggt
    17221 tttcattgtt aggtttacgc ctttaaggat ttctttttcc tcgatcgaca catgtagatt
    17281 ttttatttct aagacagaca tgcattatac ccccaaattc tctttcgttc tcactcattt
    17341 tagactaatt gagaatgaag cgcaatgacc ttccttacta aaatcaaata tcttttattt
    17401 attattaaac aattttgatt tctaaattag aataggtata aaaaagatga gaaataagcc
    17461 attacagact tatttctcat cttttttgtt ccatacctta ttttgcatca gcctttactg
    17521 gtttaacaga gccgccccat ttgtctgtta tccattcttg gacttctttg cggtgaagca
    17581 cttcaatcag ttttttgatt ttgtcactgt cttcatcacc tttacgagtc gcaataatat
    17641 tcacgtaagg agagttgtcc ttttcaagca atacggcatc ttctaatgga ttcaaccctg
    17701 cgccgtaagc gaagtttgcg ttgattgcaa ctaggtcacc ttcatcattt tggtacgtag
    17761 aagctaaaag tgatgggtcg atcgtatggt tgaacttcaa gttcttaggg ttttcttcaa
    17821 tatcatcgaa tgttgctgtt tccagatcta ctccatcttt gactttgact aagccagcat
    17881 cttgtagaat tgtgatgatg cgtccccagt ctgattcaga atttgaagta atgattgttg
    17941 caccatcttt taattcggaa acatctttga cttttttaga gtacaagccc attggttcga
    18001 tgtggacagc accggcattg acaaagtcat atcctttttc tttgatttct ttgttcaaat
    18061 aaggaatatg ctggaagtaa ttggcatcaa tgtcgccttc ttccaaagat tggttcggta
    18121 atacatagtc atcgaatttg acgatttcaa gatcgattcc ttcatctttc aataatggtt
    18181 tcacatgttc caaaatctct gcgtgcggtg tcggtgaagc gccgactttc aatgtcgtgt
    18241 cgtctgcttt atttgctgaa tctttgctgt cgctgttgtt accgcatgct gctaatccta
    18301 cagtcagcaa aagcgctgct gcaaatccaa aaattttctt tttcatatcc tacatccccc
    18361 tatatcctat tatcttttgt ctgttttctt agttaagaaa tcaccgattc cttgaacgat
    18421 aaatacgatt agtaaaataa ttaaagtcgc aacgaatgtc actgttagat taccacgctg
    18481 gtagccttcc tgccaagcaa gacctccaag tccacctgcg ccgatcgcgc cggccatcgc
    18541 agtaaatccg atcatcgaga tcgttgtaac tgttaatcca gaaatcaatg cgggaatact
    18601 ttcaggtatc aagaccttcc agatgatttc ccagtaactt gctcccattg catctgctgc
    18661 ttccagtaca ccagggctga cttcacgaaa agcaatctct actagacggg cataaaatgg
    18721 tgctgcggat aaaacaagag ctggaatggc tgcttgcgca cctagcatag tgcctacgat
    18781 tgctttagta aatggaatga tcaaaaccat caggatcata aatggcgttg accggaaaat
    18841 attactgatg atcgatacga ttccataaat gattcgagca tacggtttgt ttttgcgtcc
    18901 aatagagtaa aggatcaatc ccaaaactaa tccgatgatc gtcactaaga ccattgagat
    18961 taatgtcata tatagtgtat caaggatagc ctgattcatc gtttccgggt tgatttgttt
    19021 aaaatctaga tatgtttctg aaaagctttt atccacgatg gatcacctca attcctactc
    19081 cttgttcttt gatgaacact tctgcttgat ctaattcttt ggtttcacca gtcatcaaga
    19141 cagtcaatga accaaaggaa ccttctttca cttgtttgat ctttccatac acaacgttga
    19201 catcgatgtt atagcgtcga atcgcttggg aaatgactgg ctcgttggca tttctttcgc
    19261 taaaggtcaa agttgccaaa cggccgcttg gattttcttt gatgagttca gctaatagtt
    19321 ctgtcgaatc agcttctggt tcgatgtctt gttgtacaaa gcgtttggtt acttcttttt
    19381 tcggttgtct gaagacagtc aatacgtctc cttcttctac gactttcccc aactccatca
    19441 cagcgacttt gttgcagatt ttgcggatca cattcatttc atgtgtgatc aagacgatgg
    19501 tcaaattcaa tttcttattg atcgctaaca ataagtctaa tacttcttct gttgtttgcg
    19561 ggtccaatgc gctagtagcc tcatcacaaa gcaggatctc aggatcattt gccaatgctc
    19621 gtgcgatacc gacgcgttgt ttctgtccgc cggaaagttg tgaaggatat gcttgtccgc
    19681 gtccttctaa cccgactaaa cgcaacagtt cttctgcccg ttctttacgt ttgctctttg
    19741 gtacgccagc taattccagc ggcagctgga tattttcaag tacagttctt gaccataaca
    19801 ggttgaaatg ctggaagatc ataccgattt tctttcggaa tgtacgtaat tctttgtttt
    19861 ttaattttgt aatatcttga tcgtttacga tcacttcgcc gctcgtggga agctcaagtc
    19921 cattcaggag acggactaat gtactttttc ctgcgccaga atagccgacg attccataaa
    19981 tatccccttt ttcaacgttt agtgaaacgt cattgacggc ttggatgttt ccgtttttcc
    20041 cattgaatgt ctttttgaca ttcgttagtt ggattaatga catgccattc acatctttct
    20101 cttatgattt ttcaaacaaa aaagccttcg ccctcttgcc ttaagcaaaa ggacgaaagc
    20161 ttgaacttcc gtgttaccac cttgatttac cgttacttcg caataacagc ctcttccagt
    20221 acttggactt agcttctttc cgtatactgt cgcgctgtaa cgggcactcc cgtagtaagt
    20281 ttgcttacgc tcgcttactc tgctctaaga ccatcttcca tctcattttc gttacccatt
    20341 ttcagcaaac agggctctct tcaaacgtcc gtgacatgta ctcttctttt caaagctttc
    20401 tcgtttcata taggattgct tatgattata gcaactggaa gaatcagtgt caactttttt
    20461 caagtgattt tctgatttta taattcgtca aattcttttg gatcaacatc tttgaaactt
    20521 aagatccacg cattcatttc atctgaatca ttcaagatat tgatatcttc accgatttta
    20581 tcattaacag aagaaactgt acccgttagc gggctggcaa attcattgac cgctttttcc
    20641 gcttcaactt cgatcagagg ttcatctttt tttagcgatt gtcctgcttt tggcagattg
    20701 gcaaacgtga tgtctccaag ttcttcttgc gcttcatttg tcaaaccaac acagtattct
    20761 tttccattaa ataagatcca aaggttgtcc tttttcttca aatgcttgcc tgccatttta
    20821 tatcagttct ccttcataaa ttttttcatt gaatccaatt gcaacaagct ggtcctctcg
    20881 taagatcagt ggtcgcttga tcagcatacc atctgtcgat aagacttggc tcgcttttga
    20941 agccgtaaac tcgcctactt gatctttcag tccaagttcc cgatatttca tcccgcttgt
    21001 gttgaacagg cgacggattg gtacttgact cgtttctagc caagtctcaa tcgttgaggc
    21061 cttcggtact tctttgatca tatcaacggt ttgataacta acgccatgct ggtctaacca
    21121 cgctttggct tttttgcatg ttgaacattt cgggtaccag taaaaggtgt acaattttct
    21181 tacctccttg aagatcttgc aggtgcttca ttttggtatc tcatcgataa tatcaacccg
    21241 ataccgatca tgttccctaa aatcgaggat cctccctgac taatgaaagg caatgggatc
    21301 cctgttaaag gaagtaatcc gatatttgct ccaatattct caaagacatg taacagcagc
    21361 atcatgatca aaccgcttgc gatataggcg taaaattcat tattcgtatc gaaacacacc
    21421 cgaatcatac gatagattaa aataaaatac aagaaaatga cgaatgcacc accgataaag
    21481 ccgaagtttt caccgataac ggtaaaaatc atatcggact cacgaacagg cacatagata
    21541 ttgcttacgt tgaatccttt accaaacaag ccaccggtac caattgccaa aagtccttgg
    21601 gccggctgat aactcattcc tgacgtatca tggaaagggt ccagccatga atcaatacgt
    21661 gcaaactgat aagatttgaa tccaacttta taaagcaagt ctcttccgcc ttcagtcgta
    21721 accaagaaga tcgttcctgc tccaacgact actgccaaga ttactacagg tgcgatgatg
    21781 cgccaagaga ttccagacat caggaaaatc ccgccaaaga tcgcaagaaa gacaagcatc
    21841 gttccaaagt catcttgggc catgatcaat gctaaaaccg gtattgtcac gatcaagagc
    21901 ttcccaatca gccaaaagtc cgatttcatc gtccgttctt ttacttgcgt attatgttta
    21961 gttacgatca gtgccatcat caagatgtac gcaatcttca tcaactctgc tggctggaaa
    22021 gtaaacgcac cgaatcgaaa ccagttttta gaccctgttg aagcagctaa attctgatca
    22081 tagaatttga gcaagagtcc cattaccact aaccccaaac cataaaggta aggagtcagt
    22141 ttccagatcc atttggaatt caaatgcatg atgacaacga ttgctgccgc tccaactaaa
    22201 taccagacaa cttgcatccc tacgcctttc aagactgctg gattgttcgg atcatggcta
    22261 agtgctacat ataatgagag cagaccaatc aaacataaca gaaagactgg taaaatcacg
    22321 ccataatcaa tacgattatc gttcgaagct tttaattttt tttccatgtt tcatcccttt
    22381 tcttaaaaac catactgctg attattatag cggtccagtt agataatgga aagtctttcc
    22441 atagacattt gggaaaaatt aactttccgt ttgtgcagtt gcttgagcaa atgcagtaca
    22501 aaagcctact gcttatcttt agtgtatcat agaacttttt ttgatacatg caattatcta
    22561 ccagctgaga ttctcctcgc tgtgagaagt ttctgtgctt tcattgtatc gaaggatctc
    22621 ttcggcaatc tctagcagct gatcagaaac cagattggtt cgttggatga aataaaaata
    22681 tcgatggaaa ttcaacggag tcattggcac attttctgga acagagcgct tagacaaaat
    22741 cgagcgaccg attccttcct tcagcaacgc aacaattaca tcgttacttt tcacgatcat
    22801 cttcttatct tggatattca tctcttcaaa ataacgctgc gtgtaatgaa aaacaccaga
    22861 gtttgcttct cttacaagcc acaatttgct ttttgggtcc ccagcgatga ctagctggtc
    22921 ttccatcaaa gagtatcttt gtacgccgga agtctctaat ggcttttcga taaaaccaaa
    22981 atcggattca tgtttcgcta cttgttctaa tgattcatga gaattcgtga tttcgatctc
    23041 aaaatgcact ttaggaaacc ttttgatcag atgtttcatc aactgaggca aaagatagac
    23101 agcaaaggtg tgggaagcaa cgatccgaca tacttcttct tcattttgtt cattgagcaa
    23161 tttttgctga gtttcttccc attcttctaa caaaaccagt gtccgttcat ataataagtc
    23221 ggcttgctta gttggaagca tctcttttct tccgttacgt tgaaaaagat cgatattcaa
    23281 ttcccgctcc aactgtttga tttgattcga gactgctggt tgagataaaa acagtacttc
    23341 tgctgctctg gaaaaatttc ttgtttcata tactgctcga aaagtcgtta ataacttaaa
    23401 catggcacca cttccataac tttttatgat aattactata aaaactattt gatttaaata
    23461 tacaaaaaac catctataat agaaatgtac aacaaaacag ttgctgatcc aactagtaac
    23521 ttagatgaag acaatcagct gtctgcttgc tcgtaatcat acattcctat tcattttaca
    23581 atgtattcgt tcatcttggt actaaaatct attaaataaa aaggagaata ttatatatga
    23641 agaacttttt gaaaattatt ccgatcccga tctgcgggtt gatccttggg ttagcttccc
    23701 ttggaaatct gtttaaagac tatcaccatg ttgctttagg caatctaact ggcggtatct
    23761 ctatgattct tatgatattc atttttgtta aattgtttat cttattcgaa cacacgaaac
    23821 aaacgttgca tgatccaatc atcgcctccg tttcaccaac ttttacgatg tcactcatgg
    23881 ttatttgtac ttattttgtt tcatatgaaa gtattgcacc aattgtcaaa ttcatttggc
    23941 tgtgcgctgt tatcttccaa gtgatccttg tcttttattt caattatcac catgttatca
    24001 aagctgatct ttcaattgaa gcaatttatc caagctggtt tatcatttac gttggatttg
    24061 gggtcatcac tgtaactgcc gggaacttct tcccacttat tggaaaaatt ttcttttgga
    24121 tttcattgat ctgctatgcg ttcttattgc cgatcatcat caaacgtgtc tttttcgtaa
    24181 gaaatatggc tcatccgaca cgtccgctga ttacgatcgt tgcagcacct ggttctcttt
    24241 gcctaacagg ttatcttaaa aactttgctg aacctaattt ttatcttgtc gtagcattgt
    24301 tcgctttatc tcaagtattg tatttcgttg tgctgtctat gcttccacgt ttgttcaagc
    24361 ttcgttttta tccaagctat gcagccttta cattcccgct tgttatctct gcaacagcta
    24421 tatttactac tgttcattac ttcaacagtt taggtatcta tagtagcgta ttggatttct
    24481 taaaagttgc ggaatgtctg tttgcaagtg tagttgtctt ctacgttctt gctcattacg
    24541 tacgtttctt aattaaagaa catagcaaac atggaaaatt tgtttctaaa aaaatcgctt
    24601 aataatcgat tcaataggtt gtgaaaaagc tgttctgcat caagtaataa gaacggccaa
    24661 acaaaaacag ctgttcatgt ttttgtactg gttgggctta ttaccgcaga tgcaagcttt
    24721 tgaacaccgt ttaccaagag gtcgtaaaag aagtggtcag cttcaagaaa taagaaggaa
    24781 tttacgaaaa ttgcttctca aatttttgtg aaatttcggc ttatttccgt agaagctact
    24841 tctggaacac cgtttattcg aaacaagagg tcgtacaaga cttttgtcac gacctcttgt
    24901 tcttttgtca aaatactggt ttagtgctac tatgttcgag tatgctttta gtatttattg
    24961 acgaaaggaa ccgatcaaat gaaatcattg tcacatccaa taaaatctgt aaaggattca
    25021 gcggtccgcg aaatcttacc cggtttactg ctttcttttg ccatcgctgc cttcagcagg
    25081 cttcttgcta tttatctgcc acagttaggt ggagcgacta ttgctatttt attgggtatt
    25141 ttattaggaa atactttttt taaacagcct gttctagcaa tcggcacaaa atttgctgaa
    25201 agcaaactat tagaattctc tgttttttta ctaggtgcaa ctgtcacagc tcaaacgatt
    25261 gctcaaattg gtgtaaaagg aattgttttt gtgttactgc aaatgtcagt cactatcatc
    25321 ggtgcttact tgattggtaa aaaactgcaa ttcagcgata cttcttcttt gatgatggct
    25381 ggaggaaacg ctgtttgtgg ttcttctgct attgcttcaa tcgccccttc catccatgcg
    25441 aaagaggaag aaaaaggaca gatcatcaca ctagtcaatc tactgggtac tgtgatgatg
    25501 ttgactctcc cttttcttgg attacagttg tttggtggaa atctcattga aaaaagtgca
    25561 cttttgggtg gtacactcca atctgttggt caagtagtcg ctggggcaag cttacttgac
    25621 aattctgttg tcgaattcgc catgctattt aagattctac gtattatgat gctcgttgtt
    25681 gttgtctatc tgtttgaaaa attcatgcac aagaaacaac cagctctgtt tgaaacgcca
    25741 actgatacgc agaaaaaacg aaaattaccg cttccttggt atgtattagg gtttgtgatc
    25801 ttttgtttgg tcaacagctt gttttcttta cctgtagtta ttgaccaatc ggcacatttt
    25861 atcagcagct ggtttgagat cactgcttta gcggcaattg gtctgcgttt agactttgca
    25921 aaattcatga aagaaggtac acgcttctta tcttatggat tagctgtcgg gcttctccaa
    25981 acagtgatgg ctttactatt tatttggctg atcaaagttt agaatgaaca tgaaaaaaca
    26041 gttgtgattc tcgcatggaa caatgtccga atcacaactg tttttaattt tgcagatatc
    26101 cttctaacaa caacaattgg cgtttgattt caaccccatc tattgcgttt cccatatatc
    26161 ctgtcagttt tccattttgc ccgatcactc gatgacaagg aataacgatt ggcaatggat
    26221 tggttcgatt tgcctgccca atcgctcgca cagctttcgg actatcaaca gcaacggcta
    26281 cttctttata cgttcggatt tctccataag gaatggtcga caaagctttc cagacatttt
    26341 gctgaaaagg cgtacctctc tttatagata aaggaacatc aaattctttt ctttttcccg
    26401 caaaatattc ttcaaactgt tcttgtgtgc gcgttaaaat tcgctgattc gctgattcgc
    26461 tttctgaaat tggctgaaaa gatacagcag tcaatccgga ttcatctgca tctaaccata
    26521 aaaagccgat aggtgtgtgt attttcattt tgtttgctcc tagtcaatag actggaactt
    26581 cctccttttt ctctatccaa aactacttgc caactaataa aaattgttct taaaagtttg
    26641 ataaaccgag gaaacatggt aaactaaata cgttagttta caaaaagaat acctgttctt
    26701 ttacgtaaaa tcaaatttta aaggtgttat ttaaggttta ttaaattttg aatgtaaacc
    26761 atatataaac atacgattga aaggatagaa acgatgaaag tatcaattgt tgtcccatgt
    26821 tttaatgaag aaaaaactgt tcctcttttt tttgatgagg ttgaaaaatt caaaggtgcc
    26881 catgagttcg aatatttgtt tattgacgac ggatcaaaag acggtacgtt agcagcaatc
    26941 aagaagctag cctcagctta tccttatgtc cgctttgtct cattttccag aaattttggg
    27001 aaagaagcgg ctctgtacgc tggattgcaa gcttctacag gagatttagt gaccgtgatg
    27061 gatgttgatc tgcaagatcc gcccgaactg cttcctgaga tgatcaaccg gatcactact
    27121 tctgatatcg attgtgtcgg cacccggcgt tcgactcgtg aaggcgagcc tgcgatacgc
    27181 agtttctttg cgaaaaaatt ctaccagttg atcaataaaa tcagcgatac agaaatggtc
    27241 gatggcgctc gtgatttccg cttgatgact cgtcaaatgg tggatgccgt cctagaatta
    27301 acagaataca accgattttc aaaaggatta tttagctggg tcggatttaa aacagaatat
    27361 atttcttatg aaaaccgcga acgtgtggct ggcgaaacat cttggtcctt ctggaaatta
    27421 ttcaactact cgattgatgg tatcgtgaac ttctcggatg caccgttaac gattgcttca
    27481 tttatcggcg ctttgtcttg tgtcggttct ggtattgcaa ttttgttcat tattttgcgg
    27541 gcattgttgt ttggtgatcc tacatcagga tggccttcca tggtctcgat cttcctgttc
    27601 attggtggaa tccaattgtt atgtcttgga atcatcggga aatatattgg gaaaatcttc
    27661 ttagaaacga aacagcgtcc tgtttatatc ataagagaaa cagaaaaagt gaagccaaat
    27721 gaattgaagc ctcaccaata atatacgtca aatgaagtaa acattttttt aaatgaaaaa
    27781 ggactgtgac aaaagtcttg tcacagtcct tttttccgaa taaatggtgt tccagaagta
    27841 gcttcttcgg aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc
    27901 ttcttatttc ttgaagctga ccacttctat cacaacctct ttcatatctt attacttctt
    27961 cattgctgct tttagtgctg ctgctaatgg gctttcttct ggttcttctg cttgtgcata
    28021 ttttttcagc aagcgtcgtt cttcatgttt ggtcattttc ttcttctgct cttttttatc
    28081 aagcaatttt tctgtcgttc catcgtattt acaacggaaa taagcaccgt ttttaccttc
    28141 gatgatttcc attttccggt gacattgcgg gcatcgatga ttagagactt ttggatcttt
    28201 tcttctgcgg tagttgcaat ctgaattggt gcagacatag attttgccgt cacgtgtgtt
    28261 tttctcgcgc agattggccc cacattctgg gcatttcttt tgggtaatcg agaaatcttg
    28321 atattcttgt ttgcttgatt tgatctcatt caccaagcgt tttgtttctt tttcgattcc
    28381 attcaagaag gttcctgctt gtttctttcc tgcagcaata gcttcaaggt ctttttccca
    28441 gctttctgtc aaatctggtg ttactaaaga gggatttacc aatactaata attgcttacc
    28501 tttaggagat acttgcagtc cttgaggggt acgttccatc agttcagatt tgattagttt
    28561 ctcgataatc tctgcacgcg tagctggggt tcccagactg tatttttcca tttgaccaag
    28621 caagcttgct tctgacaatg gtttaggcgg tgcagatagt tcttttttga tggcgaaatc
    28681 gggattgatt ctcattcctt tttcccattt gactgtttcc gtctcagttt cagtttcgcc
    28741 tttccatcct gcttggacga cacgattttg ccgaaaaatg aatcgttctt taccaaattc
    28801 aaccgtaact tttgtttggc tgactttatg gggttgagca aacagtccta aaaaacgaga
    28861 gacaatcatt tgatagattt ttcgctcttc attacttaat ttttcataac gtgctctgtt
    28921 ttcagttggc aaaagtgcat ggtgatcggt cactttactg tcttggaata cttttgtctg
    28981 ttggacgatg gctccgtttt tcaaatactg cttgacctca ggagcaaagt cgctgactgc
    29041 ttgcaatcgt tctttcatcg tacttttcat gtcatttgtc agatatttgc tgtctgtacg
    29101 cggataagta acgatcttat gggtctcata caaactttgt acaagactta atgttttctt
    29161 tgcagaatag ccatagcgtt gattggcttc tctttggatc tctgttaaat cataaggcaa
    29221 tggcgcagat tccgttttta atttttcttg gacttctgtg acagttcctt tgcttgtact
    29281 gatcaattga acaagttttt gtgcttcttc ttgtgttttt aaagcaaatt gatttttttg
    29341 aagcattttg gcttgttctt ttccagcttg caacgtgatc gtgaaatacg tttgtggacg
    29401 gaactgctcg attttctttt cttggtctct cactaatgct agtgtcggtg tctgcacacg
    29461 gcctgctgat aaattatccg cgtacttcac agttaaggct ctggtcacat tcaatcctac
    29521 cagccagtcc gcttttgccc gggcaagagc agattcatac aatgcatcat attctttcgc
    29581 aggtcttagt tgcttgaatc cagttttgat tgctttatct gtttgagaag agatccataa
    29641 ccgcttcact ggtttattga aatgcacata ctctaagatc cagcgagcaa caagttcccc
    29701 ttctcttccg gcatctgtcg caatgactgc ttctgagaca tcttttcgct gagccagctg
    29761 cttgattgct tttaattgat gtcctgtttt aggaagtggt ttgattccta gttttttagg
    29821 gatcattggc aacgtgtcca tttgccagct ctgccattcc ttattcagat cttccggcat
    29881 tttcaatcct aataaatgac caagggccca agtaacgatc accttgggtc cttcataata
    29941 atttttgtgc ttttgattag caccaagcac cttgcttagg tctttcgcaa cgcttggttt
    30001 ttccgctaga attagttgtt tcataaaatt cctttctgct ttctactgtg atgggctgat
    30061 gatttttcaa caacgcaagt cccttgtatt gctgtaaatc atcgtcacac tcttcacacc
    30121 agcgctcatc tccgtcattc caaaaggcgc tgagaaagtt actttttgca ttgatatatt
    30181 cataattttg atggatttcg ttccattgtt tttcgtacgc ttgtcgtgct tcttcgaaag
    30241 actcgaatgt ttcttcttct tcaatatctt cttcccagtc ttcaaaaaac caccaaggct
    30301 cattgtcccc gaacatggta attacttgat acatactcgt gctccttctt tcagcttttc
    30361 tagactcatc ttagtcactt ttcaagaaag ttgcaagaat tttctcttat aaaagggtca
    30421 tttaatgaac atcttaaaat tgttacaaat gaatctgatt gtttaaggca aagtagcgat
    30481 aaagttattg atcatccaaa aatagctgtc ttctggatcg attgttggtt cttttgtaaa
    30541 gtatcctaaa aaacgtaagg aaagataacc gtggatcata ctacgaagtt ccctgcttcg
    30601 atgaactttt tcttctttcg ttaattgaaa agcctcaaat atctgcaaaa tgatctgatt
    30661 cgtttcatgg attccatcta ataaaacagc ttcattggta tgcggtacac tgatcagtaa
    30721 ctcatacact gcttgatttg aaaaagcaaa ggatcgataa gtttctgcat agatctgcag
    30781 tgcttcctga ccactttttc ccacaagagc ccgtcgcagc tggtcattaa gttcctgcag
    30841 aagtttcgct gttagcgctg ttttgacatc acgaatattt ttgaaatggt tatacagcga
    30901 gggagccttg atatccaagc gtttggctaa atgctgaaag gttatttgct gaacatccat
    30961 ctcttctgct agttctcgaa agcagtcgat gattttctct tgagacagat ttcttttttt
    31021 catttccatg tttcctctca taaaataaat ccttcactct ttcagtttac cacgaaaaag
    31081 aaagagaaaa aagagacttt cattgataaa ctacatattg tagtttataa taaagagctg
    31141 tagatgaaaa ttttcagaaa gaaggagtct aattggctgc aagaataaag gaaaaattat
    31201 ttaggaaaag agaaacttgg gaacaaaact tgtttgtctt atggtttgga acatttatgg
    31261 ctggtatcgg atttagttta gtcatgcctt ttatgtcact ttacatagat acgttaggta
    31321 attatagtac gtcacaattg aacttttgga gcggattgac tttttcatct actttcttgg
    31381 tgactacgct tatctctcct tggtggggaa aattagccga ccaaaaagga agaaaattga
    31441 tgctgttgcg tgcttcatta ggaatggcaa tcgtcatttc tttgatgggt tgcgtgacta
    31501 gtgtgtatca attagtcgca ttgcgtctgc ttcaagggat tttttctggt tatatcagta
    31561 atgccactgc ccttgtcgca actgggacgc caaaagaaaa aagtggtcaa gttcttggaa
    31621 ccttagcaac tggttctgtt acaggaacat tacttggacc gcttcttggc gggatctctg
    31681 cttcagcgtt tggctatcgc cctacctttt tcattacagg gatcatctta tttttcgtct
    31741 tcttgttgag tctgtttttc gtccatgaaa aatttgtccc agtgaaaaaa gaaaacatgg
    31801 tttctgccaa acagattttc aaagacttga aatatccaca cgtcgtcatc ggtatgttcg
    31861 tcacaacgat gatcatccaa gcatccaaca attccattag tccaatcatc agtctgtaca
    31921 ttcgccaatt gctgcatgga catgggaatg ttacgttgat cagcggaatc attgcttcga
    31981 tacctgggat tgccactttg atagccgctc caagatttgg tcgcttaggt gacaagattg
    32041 ggagtgaaag aatacttgcg attggtttgg gttttgcgat tctggtttat atcccaatgg
    32101 cattcgtcac gaatgtttgg caattagctg ccttacgttt tttgattggt atctctgatg
    32161 cttgtctgct ccctgctgtc caagcattga tcacaaaata ttcgcctcat caagctgctg
    32221 gacggatctt cagttataac caatctttcc aagcaactgg taacgtagta gggcctatga
    32281 tcggctccag tgtttccagt gcatttggtt atcgtggcgt ctttctttcg acttctcttc
    32341 ttgttttggt caacttcttg cttgtacgac acaatacaca agagatccat cagcaagaaa
    32401 ctgcagaaga aaaagcttca ggagtctcca tagcttcagg gcactctcct tctcatagac
    32461 aataagcgta aatgaaaaac actgcttgaa cagttgtcac agactgtcct tgcagtgttt
    32521 ttgtttaggc ctgttttttc aagaactcag ataagaggaa taatcccttt tccaaggttt
    32581 cttttttaca acagtaacct agtcgggcat ggttcggtaa atcaaaggca cttcctggta
    32641 ctaatagtac tcctgtttct tctaataaat tgatacaaaa tgtctggtca tcttcttcga
    32701 tggctaattt aatgaaagaa gtagagacgt aattgggagc gacaagttca actttcggtt
    32761 cattggctac ccattgtttt aaaatcacaa ggttctctgt aatgatcttt ttgtttcgcg
    32821 ccaatatttt ttctttattc ttcaatgcat gtacagcaag atcatctgac aggactccgc
    32881 cgcaaatcat cgtgtagtct cgatactttc gaaaaacttc tgccaactca ggtccagtag
    32941 ctgtccagcc aatacgaata cctggaatgg agtaggtttt ggatagggaa ttggttgcta
    33001 tacctttttc atagacatct acgatcgaca agaattcacc tttatccgtt aaaggcgcat
    33061 atacttcatc tatcaataca tatgcatcta ctgtacgagc aatttctgcg attttttcta
    33121 aagttttccg atctagcaat gtgcctgtag gattgtttgc actattcaaa cagatgattt
    33181 ttgtttcagg tttgactaag gcttctagct gttctaaatc aaactgccaa tcttcttctt
    33241 ccttcaaatg aacaaagtca acagcggcac ctaaagattt ggggatgtca tataattgct
    33301 gataagtagg aagcatcgaa acaacatggt ctcctgcttc cactaacgcg tataaagcta
    33361 aaagattagc accagtcgct ccattcgttt gtaagatgtt ttccggatcg acggtttgat
    33421 aaagagaagc gacttcttgt ttgaaaacat ctgatccttc gatccagcca tagtccaatg
    33481 gttcgttttt gcgtttttcg aaaaatttac ttacgctagt cccatctaag ccaacgagtt
    33541 cgtctaatgt caatgcttcg atcgagcttt gtgcaatatc atataccgct ttcttttccc
    33601 atttatttag ccattcttct acaccgaaat ttgcgatttt catgtttctt tcccccttat
    33661 atcttgatcc agcctacagc aagcaacccg attgaaagca gtgcggccac acaaatcagc
    33721 ccaatcatta ctttttcttt tcccgtaatt tgatgtttgg tttcacgaca agcgatccag
    33781 tagaagcaaa aaccaggaag ataggcaata ctacataaaa ggacataaga aaatcctgct
    33841 aaaatgatgc ccatcaattc aaaaactaca gcaatcccac cgatcactac ttgttttttg
    33901 attccttctt ttaaagaaaa ctggacttga tagatcccga caagtagata acaaatcaaa
    33961 attgccgctg tacataaaga ataggcaaat tgataggcat aatcagtaac taagaaagtc
    34021 aataagaata gattagtcaa gattgcagta acaactaacg aataagttgg ggcatttttt
    34081 ttattcgttt ttccccaacc tttcggcaac agaccgtctc gagccatcaa catcgttgtt
    34141 tctgctggga gcatcgtcca agacaaccaa gaaccaatga tcgaaataat caagccgata
    34201 ttgatcagcg ctgctcccca atttcccacg attcctttta aaatatttgc catagcaggt
    34261 tgtgaaatag ttgctaactc ttcttgagat aagacgccgt atggcaagat cgaagcaagg
    34321 atatagataa acaataaaca aagcagacca atgatcgatg cttgctgggc ttctgaacgt
    34381 ttcttcgcgc ggttggctaa aacacttgcg ccttcgatcc ctacaaagac ccacatcata
    34441 accatcaaac aacctttaat ctgcaaccag atcgttcctt gctgccctgt tccattcact
    34501 agattatcag atacgttacc ccaaaaatca gcagtgaata tattcacttt aaatgcgata
    34561 aatacaaata ttaaaaatag gaataatggc actaatttac agatcgtcac gattgtattg
    34621 atgacgctcg ctccttcaac tccattgttt actaataaag tcagacccca aataaaaata
    34681 cttgcaaata aaatagaagg aacattttgt ccacctttga atatcgggaa aaattctccc
    34741 atggcgctca tcagcatcgt tgcaaaagca acatttccca gccacgcaga caaccagtaa
    34801 ccccaaccac tgatgaagcc acctaattta ccaaatgaag cttccgcgta accgaaaata
    34861 ccaccatcta gatctggacg cttttctcct aaatgattca gcgataatac caaagctaaa
    34921 ataccaatcc caacgattat ccatgaaagg atcgctggcc ctggagctgc agaactagcc
    34981 agatcacttg tgatcccaaa cacaccgctt ccgattgacg aactgacaac cagagcaacc
    35041 agtccccatt tgctaatacc cgtcgatttc ttttccattt ccatcaccct cacttataaa
    35101 attgaacaaa tcacttgcaa aagattccct tctattttgc aactgctctt gcttaacaaa
    35161 aagcaacttt attcatatta ttggatatta tgaacttttt acacaagtat ttcaagtata
    35221 aaatgaataa aaattaattt tattgcaaaa aaacagctat gctatccaaa agaaaaggtg
    35281 tttcttttgt tagccatagc tgttttgatt catttaatat cgcttaaacg tattgtaaaa
    35341 cttttgtttc ttgatatgcg tggtcgatca aaccaccacc cagacattcc attccatcat
    35401 aaaatacaac tgcttgtcca ggagtgattg cacgtacagg ttcgtcgaat acgacttctg
    35461 cacgattatc atccaataag cgaactgtca caggaacgtc ttgctgacga taacggaatt
    35521 tcgctgtaca cttgaattct tttggcattg gttcattcgt cgtaaagtgg atttcacttg
    35581 catccaagct tgtggcatac aatgcaggat gatggaaccc ttgccccaca tataatgtgt
    35641 ttgtcgctag atcttttcct acaacaaacc agggttcttg cgaatctcct ccgccaccga
    35701 tccctagtcc ttgacgttga ccaatcgtat aatatatcaa gccagcatgt tgtcctttga
    35761 cttcaccatc aagagtgacc atatttcctt tttttgctgg cagatagttg ctcaaaaatt
    35821 gtttgaagtt cttttcacca ataaagcaga ttcccgttga atcttttttc ttagcagtcg
    35881 caaggcctgc gcgttcagca attgcccgta cttctgattt ttccatgccg cctagaggga
    35941 acatcgtttt tgaaagctgt tcttgtgata attggctaag gaagtaagtt tgatctttat
    36001 tattgtctac tccacgaagc atgtggacca cgccgttttc atcgcgagtg acttgcgcat
    36061 aatgtccagt agctacataa tcggcgccaa gatccattgc ataatccaag aatgctttga
    36121 atttgatttc tttgttgcac atcacgtctg ggttaggtgt acgtcctgcg cggtattcgg
    36181 ctaggaaata ttcaaataca cgatcccaat attctttttc aaaattcaca gagtaataag
    36241 gaatcccgat ttgagcagct actttcgcta catccttata atcttccgtg gccgtgcata
    36301 caccgttttc gtccgtgtca tcccaatttt tcatgaatat tccgatcacg tcgtatcctt
    36361 gttcctttaa caaaagtgct gttacagatg agtcgacacc gccgctcatc cctacgacaa
    36421 cgcgtgtttt gctgttgtct gtcatgatat caccatcatt tcaattagat tctgaaacat
    36481 ctacgagttg aaggtcgcaa cttttcagta ttttacatta tacgtcatat ttctgaaaat
    36541 ttcatccctt aattatcaag tcgtaaaaat actgttctac tggacctatt cagtacacaa
    36601 acatgatagt caaccaaaaa atcactcatc ttttgagcat tcgatataaa aaattggcat
    36661 actcataaaa agggccagcc tttcggcaat aaggaataat tttataaatt gtgaaaaact
    36721 atttttcaaa attgcttctt atcgcttaaa gctgcacgtc tcatttacaa ccttttcttt
    36781 ttagatgtta tgagttgctc agataagtga ttttgtttac tctaagaaaa aagggaggca
    36841 aaagatacat gaattacttt atcagtgata tgcacttttt ccatgaacgc ttattaggaa
    36901 aaaacgactt tgctccgcgg ctctttgcaa atgtggatga aatgaaccaa cagttgatca
    36961 ctagctggaa tgcagtaata aaagaaaccg accatgttta tcatctaggc gaccttgcta
    37021 tgcatccgca atatgaaaaa gcaaatgcag aaattttgga actggtcact caactgcaag
    37081 gaacaatcca cttgatcaaa gggaaccatg acagtcgcgc ttttttccgt tatttagaaa
    37141 aaaatgatcc taggcttact gacggaagtc ccaaatttta tttttatgat gtaggtgcca
    37201 tcgttaaatt cgaccatcaa caatattact tgactcatta tccgatgttg ctcgggccta
    37261 acgacactat ccgcaatctt catggacata tccatcatta cagcgtacct atcgagaacg
    37321 atgtcaatgt aggcgtagac gcaccggaaa gagaactctt aaaggaaaaa ctaccatttg
    37381 gcactccgtt atctgagcaa caaatcaaag aaatctatga agcaaagagg aagtacgata
    37441 ggtcgtgaaa aaactgccct atatcaagta ttaaacaaat aaagataatt actcatattt
    37501 ttcatttgag taggcttatt gccacaggaa cattaggatc agaaatggag ttgtaacaag
    37561 acttctgtca caactccatt ccatactatt cgtctttttg ctcatggtgt tcttttacca
    37621 gttttttctg taattcttta tcataatttc gggcagctga cgttaaaaat aaaacgattc
    37681 cgcataataa aatcccgatt ccggaaaaca agaacatttt ttctacccct aaacgatcag
    37741 ctaatggtcc tgcaaataat aatccaattg gacctgccag gttcatcaaa gagttgaagt
    37801 tccctaagac acgacctaaa acattcgatt cataactctg ctggatcatt gccatcagca
    37861 gtgtattgaa atatggcgtt gcacaacctg ctcctgcatt caagataaga aagtacaaga
    37921 acccttgact atcgtttgga accaatccgc ttgcaccgat cgtcaggccc ataatgatgt
    37981 aggctataat aataggtttc attcggtctt tccatgttcc tataaaactg atcagtgcac
    38041 cgcctaagag cattccgact gcgtagacta cttccactgc tcccgcctgt ccaacggaac
    38101 caccaaagta atctagcgtc atcaatggat acagactgat tgctggcatg aacaacagca
    38161 tcacaaatgc accattgatc gttatgtacc ataacccttt attatccatc aattgctgca
    38221 gcccaaattt tgcatcttta aaaatagtaa gcggaacttc caatatttca ggagaaactt
    38281 ttgggatctt cacaaaaatc aataagccga caccaaaaac tgcccccaat acatctaata
    38341 aaataaggta attgacagga acgactgaaa acaaagccgc acctagagct ggcgcaatga
    38401 tgaaattcgc tgattggacc attcctaact gtccattcgt ccttgttatg tgagaagatg
    38461 gaacgattgt cggcataatc gactggatcg ttggcatttg gaatgtttgt gcgaccgaac
    38521 gcataaacaa agacacaaaa actagccaaa tcgggaagga ttctgaaatc gttccgacta
    38581 ctgctaaaat aagcgcgaaa atcgctacaa taatatccgt gacaatcaaa agagcttttt
    38641 tatcccagcg atccactagc ggtcctacaa atgggcttag taaaaccatg ggaatcattc
    38701 ctagtaaagt agcaaaactc aaaattgtcg ccgaaccggt ttctcttgtc aaataccaga
    38761 tgatcgcata ttggacgacc atactagtga ttcctgacaa aaactgtccc gataaaaaga
    38821 acaaataatt tctcttccaa ttcgttaaaa cgaccgactg ttcattttcc ataaacatcc
    38881 ctcttttctt tttcattttg tatagtgttt gcacaacaat cattatacgg gattcttttt
    38941 tcaaaaaata cctttttccc taaataacaa tcgtttcaat ttaaatttct ttacttagaa
    39001 acaataaatc agcaaaatat taaaaataac aacatgtgtt aaatttaaaa ataaaaaaga
    39061 caactaattt gcgtcgtcct tacaaagtta gtcatctaac aataggccgt ttcaaaacta
    39121 gttgtctttt tatatccaca gatcattcga ctttttcgaa taacccacgg ctaatcatcc
    39181 catccatcag aaaataaaat ttatcttggc tcattttctg ttttggatct ttgaaaatat
    39241 tgactgcttt caatccattt gctgtagtga tcgacatttt caatgtttga atatcttttg
    39301 atacagtgat ttttaatttg aatccttctt tgctttgcgg cgtggcatct aatggtcgga
    39361 tcaattgata attgcctccg tttgtttctg taaatccatt atcgcgcaat gtatatcttt
    39421 ttgcatcagg atgcaaacga tagctaattg gtgcgcccag taccgttgct gttgtttcaa
    39481 atgccatgat tttcacctcg ataaaattag tttctcttta ttataaaagt ttatcctgat
    39541 ttttactagg tttttcagtt aagttcccta aaaaatgaag gcgttctctc agacatctgt
    39601 tcatataaga atgacttcac tagttatttt ttgacagcac gaactaattg atcaaggaaa
    39661 tactggatgt cgtcttcggt atttcccaaa ccaaagctga tacggatgga ttcttttagt
    39721 atcggtgtat cttctccata cattgcttct aatacgtgtg aaggctcgat atttcctgct
    39781 gtacaagctg aaccagtaga aatagcgacc ccattaagat caagcttcat cagtagcaaa
    39841 tcatttgctt cgccaattaa gcgaagattc aacacatgag ggagtttatt cgttaaatca
    39901 ccatttactt gataagggat ttcgtttgct tctaatgtat caagaatcaa ttgctggaat
    39961 cctttatatt tgttgttacg tgcttcacgt tcttcaggtg acagcagttc aactgctttt
    40021 gccatacccc agatccctgc taaattttct gtacctgcac gccgcttttc ttcctgctcc
    40081 ccgcctaaaa gcaatgcagg gatatttact ccgtctcgtt tatataagaa accgacgcct
    40141 tttggcccat tgattttatg agcagagata cttaataaat cgattcctaa ttcttctgga
    40201 tggatcgttt ggtttccgta cgcttgtacc gcatctgtat ggaaataagc tggatgatcc
    40261 gctaaaagtt caccgatttc tttgattggc atcaaatttc cgatttcatt gttcccgtac
    40321 atcacagaga ccaagatcgt atcttctcgt aacgcttttt tgacctcttc gatcgacaga
    40381 ttcccttttt catctacagg cagatatgta acgtcaaacc cctttgtctc taaatatttc
    40441 atggtattaa gaacagcagg atgttcgata gctgtagtga ttagatgtct gccttctttt
    40501 tgacgcgaga acgctgtttc taagatagct gtattatccc cttctgttcc cccactgtta
    40561 aagatgatct cgtgaggttt tgcttgcaga ctgtctgcaa tgatttgtct tgctgtttcc
    40621 aatttttcat gcgcgtggcg accaaatcca tgtatgcttg aaggatttcc aaacgtgtct
    40681 tgcatgattg ccgtcatcgc ttcaattact tgtggatgca tgggggtagt tgccgcatga
    40741 tccagataaa tagttttcac ttcattcatc ccttccaaat tattgctagg cacattctag
    40801 cataaaatag cgaacaaacc aaagaagagc tcatttttgt ctagaggacg tttcatgggc
    40861 atagaaaaaa ggagctgtga caaaagtctt gtcgcagctc cttgaagctg ctcacttctg
    40921 tcacaacttc tttccctctt ttttttgttt attcgaattc tttttcttcc agacggaata
    40981 acggactcat cggtgtattt tcatggatgc gtttgaccgc ttcacccatt aattctgaac
    41041 atgtaacgat tgttaagttt tctggatgac gattttcttc tgtgaaaaca gaatcagtga
    41101 tacaaatatc tttgattggt gcattgtcta aaatactttt cgcatgatcg gacaataatc
    41161 catgtgacgc acaggcatat acttcttttg cgccgttttc ttttaataca tttgctgctc
    41221 gtgcaagcgt tgctcctgtg ttcaagatat catctaccat gatacatgtc tttccttgaa
    41281 catcaccgat cacataacca ccctcagttg aagcatcatc tgcatgatct acgatggcta
    41341 atgtgctgtt caaatattct gaaagactac gtgcacgctg aacaccgctg ttttttggcg
    41401 aaacaactac gatgtcatct cccgtcagac ctaattgacg gtaataatga gcaaacaacg
    41461 gcatcgtaaa taaattatct acaggaatat caaagaatcc ttgtacttga accgtgtgca
    41521 gatctaaagt cagtactcga gttgcccctg cttcttcgat caagttagcg atcaatttcg
    41581 cagtgatcgg ttcatgaggt ttcgccgtgc ggtcttgcct agcataccca tagtaaggca
    41641 atacgacatt gatcgttttc gcacttgctc gcttcattgc atcgatcatg atcagtagtt
    41701 ccatatagta atcattgaca ggtgcattcg ttgattgaac gatatacaca tgatcccctc
    41761 gaatactttc ttcgatattg atggaaatct cgccatcact aaattgcttc acgacgcttt
    41821 tacctaattc tgttccgaac actttcgcga ttttttcagc taatggacgg ttgccattca
    41881 aactaaaaat cttcaacgtg tcatcctgat acttattact catgtagtcc tccccaaaac
    41941 ttctctattc ttcgatagtt taacatattt ttccaagatt ttctgttaat ttcagcaaat
    42001 tcttaaggaa gtgaaactgt tccgttactc caactggcag gaatgacttt atgataatcg
    42061 ccccagttgt cgtgtacttt gtaataacgt tctcctgttt cagtttcaaa ataagcgtac
    42121 gctgtcaccc aatgattgcc atatgtactg ccaagtactt tcaaaatacc aatcatcaca
    42181 ggtttgcctt cacgtatacg ttttgtagct cgctgccatg aacctagaag agttgctcgt
    42241 gcacgctcat gattgccatt cttctttaaa aaactagaaa tccctgtact aacctgaaaa
    42301 ggaatcgtag gaagtcctag tggttggatc tggctacgca atgccttcgt caaaaccaac
    42361 gaatcagccg tatttttctt gcgaatctca tttggaagca tccacccctt acgatagtct
    42421 tgataatagg caaggagaac tgctgccgcg taagtaccac aaagatagcc tgagggggta
    42481 gcccatttct tatacctcaa aaaacgttct tctggcaatc ccacccagca atttgcgagt
    42541 tcctttcctg accttttttc ttcgatcaaa acgggaatat cttcttttct gttgatccat
    42601 ctagataact cgattggctc ttcattgatc agcttccatt ttttacaaga aagaccctct
    42661 tgataagtat attttgctgg agaatattct accggatgtc ctgattgatc gataatcaca
    42721 taactttgat cttcaaaaac cagagctaaa aaaagcaatt tactttttct tgactgcagc
    42781 tgctgagtgc tggctaattt tttaattttt ttatctactt tgaaatataa aggtttatcc
    42841 actacttgat tcagttggga tcgcctcctt tttttcatta taagtgaagg attgaaaaaa
    42901 acaaatcagt ttgattgttc gaattttcaa ttttttatgt tctatttgtt aattccttca
    42961 ttttgtaaga taatgggcac acgaaacaaa aaaggagcga tcgatgaatg aacaaagaac
    43021 gattgaaaca agatttctat gaagcagtca atgaagaatg gcaaaaaaca gcaaaaatcc
    43081 ctgcagataa accagctaca ggcggtttcc aagatttagt tgacgggatc gaccaacttt
    43141 tgatgggaga aacagacgat ctgcttgccc atccagaaaa agtgtcttca gaaaaaatga
    43201 aacatttcct tgcatattat caactggcaa acgattatga ccgaagaaat gagttgcatg
    43261 ctcaaccgct tcaacctata atcgaaaaag ttgaatcatt gcagtctttc gacgaattga
    43321 ataagcaatt tccagaatgg ctgttggata cattaccatt gccatttaat ctggatgtag
    43381 atgcagatat gaagaatgcg caaacgaacg ccttctttgc ttatccgcct tctttattcc
    43441 ttccagataa gacgtattat gcatcagacc atcccaacgg tgcacaacta ataggcgttt
    43501 tctttgatat ggctgttcag ttattagagc ttgccggcaa agaaaaaagt caggcagaag
    43561 caatcacgga acaagcagtg caatttgata aattgattgc tcctcatgta aaaagtgcag
    43621 aagaaaacgc agattacagc aaaatgtata atccgcaatc ttttgataca tttattaaat
    43681 acacagataa actggacttg aaaaaacttg tcacgggttt gattgggaca actccagata
    43741 aagtgatcgt aactgatcca gtgtacttcg atcatttgaa tgacatcctg acagaagctc
    43801 attttccgtt attcaaaagc tggatgatca ttaaaacagt acgttctctt agtggttatc
    43861 tatcagaaga attccgccaa gtcagcggaa tctattctcg cgtattgtct ggaacagacg
    43921 aagcaatgcc tcagaaaaaa gcagcgtatt accttgcctc tggacaattc gatcaagtag
    43981 ttggcgatta ctacggaaaa aaatattttg gcgaagctgc taaaaaagac gtagaacaaa
    44041 tggtgaaaaa aatgatcgcc gtttataaaa aacgtctaga agaaaatgac tggcttgggg
    44101 atgcaacaaa agaaaaagcc gtcataaaat taaataactt aggtatccaa gtaggctacc
    44161 ccgataagat cccagcatta ttcgaccagt tcaaaacagt acctgcagat cagggtggta
    44221 ctttgctttc aaatactctt gttttcagca aactcacatt aaaagatcgt ttcagcaaat
    44281 ggaacaaacc agtggatcgt tcagaatggg aaatgagtgc tgacacggtc aatgcttatt
    44341 atcatccatt tcggaatgtg atcgtctttc cggcagcgat ccttcaagca ccattttaca
    44401 gtctagaaca atcaagctct gctaattatg gtggaatcgg agctgttatc gctcatgaga
    44461 tttcccatgc atttgataat aacggcgctt tatttgacga atatggcaac ttgaacaact
    44521 ggtggacaga agaagatctt acacaatttc aaaacaaagc acaagcaatg atcgaagaat
    44581 tcgatggtct gccttttgct gacggaaaag taaacggaaa attaaccgtt tcggaaaata
    44641 ttgctgatgc aggcggtcta agctgtgcat tggaagcagc aaaaacggaa tcagatcttt
    44701 ctatcgagga attctttgtc aattgggcaa cgatttggcg tacaaaagca aaaaaagaat
    44761 accaacaact tttactgcaa atcgatgtcc atgcccctgc taaactccga gcaaacgtgc
    44821 aaccaaaaaa ccttgacgaa ttctatgaaa catttggtat cacggaacaa gacgctatgt
    44881 atctagcgcc agaaaaaaga gtgcgaatct ggtaagaggt tgtgccagaa gtggtcagct
    44941 tcaagaaata agaaggaatt tgggaaaatc gcttctcaaa tttttgtgac atttcggctt
    45001 atttttcgaa gaagttactt ctggaatacc atgtattaga ggttgtgaaa aagctgctta
    45061 gtttcaagta gtaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttga
    45121 cttactaccg agaaactggc ttttgaacac cgtttatcga gaggttgtga aatcagcgtc
    45181 ctgacctgag tattagagaa aaaaacggaa aaatagttcc tcatattttg ccgtttgttg
    45241 agctaatacc gagggtcagc tgattgaaca ccgtttagtg gagagattgt gaaaaagctg
    45301 tcctgcatca agtaataaga acagccaatc aaaagcagca aggagaacct catctataaa
    45361 aagatagaag gaagtgagac atctattggt ctcatttcct tctattttta ctttcctcag
    45421 tcaattgttt tacatcataa aaggagaaat tccacttatt tttttcgctg tttcatgaca
    45481 taaacaacta caccgattaa caaaattcct ccagctaatg ctagcaacgg gttattaaat
    45541 tctcccgttt ttgggagtcc ttgagtgcca tttttttcac tttcttttgc tgtcctatcc
    45601 ggatgagaag gttttccatt tggttttcca tttggtgatt catttcctgg attttctctt
    45661 tctggtttgt catttcccgg gggcacatcg gcagtttctc gcacagtgat cgctttttgc
    45721 tgggtaacag attctgtatg agcagaccat ttttccccat caaatatttc cgaacgataa
    45781 gtgacttcta gcgtatattc tccttttgaa ggtgctgaaa aaatagcggt atgattagaa
    45841 aattgagcac ttgactgtgt ttcaacgatt tggatctttt ctgggaaata acgcatatct
    45901 tcaaagacag gcgttccttg atttggtgtg aaagttgaca caaaaactgt aaaaggcttc
    45961 agttcaatga tatttccttc tgcattctcc gccacaccat ttgcttcttg tataataatc
    46021 ggattatttt gttcatttgg ttttgtctga tttggttctt gtacgattgc atactctgga
    46081 tattccgaag acggtgaggt ttctccgtca aaaatagcac ctacagataa tggaagacca
    46141 tccactcttt ccttttctgt ttgacgattc cccggtttaa tctgcatcag tccatgaaca
    46201 tggatacttc cattttcata gcgcgtaaca ggctgctgac cttctttcga acatgtattt
    46261 agagactcga tccaatcttt tgatatggta tgtccaagag tattttctga ttgtttctct
    46321 cgttcattcc aatagtagtt caacgagtgg tagatttgat cttcgttttg attcaatggg
    46381 cggatctgct ctctcatctc aagattctct gttcggaaag ataagatacc agaagcatgg
    46441 tagtctgtgt ttcctgctga agaggtataa agagccacat tatatgaacc gttattaaaa
    46501 cttgtactgc tattgacgat aatatctgtt ccgctattgg aatcgattcc tttcgcaaga
    46561 ttttcgaaag acaaactatt gatcaattca tgagggcccg ttaaagaaga accgcccatt
    46621 ttgaatccat taccgttacc ttctccctca attcctggta cccagccgtt tcgataagca
    46681 actgagtttt tgatgattac ttttccgatc gaacctgttt catttttagc aaaaaggtcc
    46741 caaccgtcat ctgcattatg atacgcgata caaccgtcaa atacatttcc ttctcctatt
    46801 gtcagtttgg ccgcaaatcc atcggcatct tcaaatcctg gatcggcatt ttcaaaagat
    46861 gtacagttct tgactaagtt atgcgcgggc catgcttcga aaggatcatt tgagctaccg
    46921 ctgatttgga ttcctgtatt tccattccga taagcatgaa tctcttcgat caaattatga
    46981 tttccagaaa tctgtaatcc tttttgcata gctgcactat ttgtaatgtc aaaccctcgt
    47041 aaaccccagt agtgactgaa cagtgtcatc ccatttccag ttcctttaaa atcaagtaca
    47101 ggacgcttgt tttctggatt tttctctgca atcaactgaa tcgggttttc tcttgtcccg
    47161 ttaacgcctt ttggaatcgt caatcctctt tcgaaggaat aagttccagg cgccatgacg
    47221 attgtctgcc cagcatatgc gtatctcaat gcttgtgtca gagataatgg attttgtctg
    47281 cttccgtttc ctgcatctga cccttctggt gttacataaa ctgtcgaata tttaggttta
    47341 cggtagtcca ctgtatgaag aatctctttt gtttcataac tagtcatctc tacgtattct
    47401 tctggcggat agtttttatc tggcgtaaaa ctgaaagtaa attcgttcgt tccttccatc
    47461 agttttgtct gataatcaaa ttcccgattc gcagtgatag ccaagttttt tgcgttctta
    47521 agcacctctc ctgttgcttt atttttaatt gtcaataatc cgtcagcatt tgctctaaag
    47581 gacatagtat gatcttggtt tcctgaatga ttactagagg tcacagtgta aatcggatca
    47641 atcaagcgag taggtctttc ttgcccagga ggatctgtcg caggatctga gtaagcaact
    47701 tcgattcctt ctaccttgat cgtcatattc cttgctgcag caaatcctgc ataaataacc
    47761 gattcatctg aagcgtataa tttttcccag tcatacatga tgtcagaagc catttcttct
    47821 cctgtttcag gatcgtaata atatgcctct aaaccagtat tcgttttttt catctctacg
    47881 tccagtttag taatcgagtt cgtggccgga aacagattcc catgaccgtt tccgaatata
    47941 ttataagaac ctgtttcttt ttccaagaac cctgctgaag tctctaaagg tgttgtttcc
    48001 actgagacac tcccaggagc tggcgcagct tgcggatctt cagatgatat tcctgtgatt
    48061 gcccgtgtcc ccaatccaag tcgcatcgta tatttgctgc cgctcgtgtt gaccacttct
    48121 tgtctatccg agtcccagac atattcgatc cgactgccta gtatggcaaa tgaatttgaa
    48181 taaaatgcct gcccaaacaa atgatttttt ggtactgcgt ctcttaccat taaagcaaat
    48241 ccttcttggg cattcgtata ggtccaagat tctatttcga tcgtcgcccg catcctaaag
    48301 ttgatgtctt tggggattgc ttgataataa taggtcattc catctgatcc gcttgactgg
    48361 aattttccgc cgttattcgt ggaagagagc cgcacgccgt cttctaatgg ttcaatggca
    48421 tacctactag cacttgtgct ttctccgaaa acaacccctt tccactcttc atttgcttct
    48481 tcagattgct tttttgtctt ttcattttct gattctttca tcataggatc atttccttct
    48541 tccgtatcca tatcttcact agttcttggt atttcctcca ttgttgacat cccttcaatg
    48601 ttcacgtgtg cattttcttc gttcacagac tgttgttcgt tagcagcaac ttcaacgaca
    48661 gaaattaaaa ccggggtact gagcataagg agattcatca ttagcgcact gtattttata
    48721 cgttttttta acaagtgtat tcctcccgta acaaattaga aagcgtttac ttaaagtatt
    48781 acctaattta tttttactg
//
LOCUS       DAZKCY010000009        43937 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000009 DAZKCY010000000
VERSION     DAZKCY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.009"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..2714
                     /gene="adhE"
                     /locus_tag="ACG8XC_000456"
                     /gene_synonym="adhC"
     CDS             117..2714
                     /gene="adhE"
                     /locus_tag="ACG8XC_000456"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP9190180.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2943..4049
                     /locus_tag="ACG8XC_000457"
     CDS             2943..4049
                     /locus_tag="ACG8XC_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9190181.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4066..5094
                     /locus_tag="ACG8XC_000458"
     CDS             4066..5094
                     /locus_tag="ACG8XC_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFP9190182.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5100..5579
                     /locus_tag="ACG8XC_000459"
     CDS             5100..5579
                     /locus_tag="ACG8XC_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP9190183.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5595..6890
                     /locus_tag="ACG8XC_000460"
     CDS             5595..6890
                     /locus_tag="ACG8XC_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFP9190184.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6901..7725
                     /locus_tag="ACG8XC_000461"
     CDS             6901..7725
                     /locus_tag="ACG8XC_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9190185.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7890..8789
                     /locus_tag="ACG8XC_000462"
     CDS             7890..8789
                     /locus_tag="ACG8XC_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9190186.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8915..9784)
                     /locus_tag="ACG8XC_000463"
     CDS             complement(8915..9784)
                     /locus_tag="ACG8XC_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9190187.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9937..11397
                     /gene="rhaB"
                     /locus_tag="ACG8XC_000464"
     CDS             9937..11397
                     /gene="rhaB"
                     /locus_tag="ACG8XC_000464"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP9190188.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11394..12650
                     /gene="rhaA"
                     /locus_tag="ACG8XC_000465"
     CDS             11394..12650
                     /gene="rhaA"
                     /locus_tag="ACG8XC_000465"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP9190189.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12664..13509
                     /gene="rhaD"
                     /locus_tag="ACG8XC_000466"
     CDS             12664..13509
                     /gene="rhaD"
                     /locus_tag="ACG8XC_000466"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP9190190.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13511..13822
                     /gene="rhaM"
                     /locus_tag="ACG8XC_000467"
     CDS             13511..13822
                     /gene="rhaM"
                     /locus_tag="ACG8XC_000467"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP9190191.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13904..15055
                     /gene="fucO"
                     /locus_tag="ACG8XC_000468"
     CDS             13904..15055
                     /gene="fucO"
                     /locus_tag="ACG8XC_000468"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP9190192.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15182..15940
                     /locus_tag="ACG8XC_000469"
     CDS             15182..15940
                     /locus_tag="ACG8XC_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP9190193.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            16040..16684
                     /locus_tag="ACG8XC_000470"
     CDS             16040..16684
                     /locus_tag="ACG8XC_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9190194.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16691..17698
                     /locus_tag="ACG8XC_000471"
     CDS             16691..17698
                     /locus_tag="ACG8XC_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFP9190195.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17714..18556
                     /gene="kduI"
                     /locus_tag="ACG8XC_000472"
     CDS             17714..18556
                     /gene="kduI"
                     /locus_tag="ACG8XC_000472"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP9190196.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18576..19379
                     /locus_tag="ACG8XC_000473"
     CDS             18576..19379
                     /locus_tag="ACG8XC_000473"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFP9190197.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19628..20692)
                     /gene="fni"
                     /locus_tag="ACG8XC_000474"
     CDS             complement(19628..20692)
                     /gene="fni"
                     /locus_tag="ACG8XC_000474"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP9190198.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20689..21774)
                     /locus_tag="ACG8XC_000475"
     CDS             complement(20689..21774)
                     /locus_tag="ACG8XC_000475"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFP9190199.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21787..22764)
                     /gene="mvaD"
                     /locus_tag="ACG8XC_000476"
     CDS             complement(21787..22764)
                     /gene="mvaD"
                     /locus_tag="ACG8XC_000476"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFP9190200.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22757..23701)
                     /gene="mvk"
                     /locus_tag="ACG8XC_000477"
     CDS             complement(22757..23701)
                     /gene="mvk"
                     /locus_tag="ACG8XC_000477"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFP9190201.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            24017..24676
                     /locus_tag="ACG8XC_000478"
     CDS             24017..24676
                     /locus_tag="ACG8XC_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9190202.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24761..25666
                     /locus_tag="ACG8XC_000479"
     CDS             24761..25666
                     /locus_tag="ACG8XC_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9190203.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25668..26300)
                     /locus_tag="ACG8XC_000480"
     CDS             complement(25668..26300)
                     /locus_tag="ACG8XC_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9190204.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    26451..26576
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26620..27144
                     /gene="infC"
                     /locus_tag="ACG8XC_000481"
     CDS             26620..27144
                     /gene="infC"
                     /locus_tag="ACG8XC_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP9190205.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27216..27416
                     /gene="rpmI"
                     /locus_tag="ACG8XC_000482"
     CDS             27216..27416
                     /gene="rpmI"
                     /locus_tag="ACG8XC_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP9190206.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27469..27828
                     /gene="rplT"
                     /locus_tag="ACG8XC_000483"
     CDS             27469..27828
                     /gene="rplT"
                     /locus_tag="ACG8XC_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP9190207.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28080..29417
                     /locus_tag="ACG8XC_000484"
     CDS             28080..29417
                     /locus_tag="ACG8XC_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFP9190208.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29465..31594
                     /locus_tag="ACG8XC_000485"
     CDS             29465..31594
                     /locus_tag="ACG8XC_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFP9190209.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31569..32258
                     /locus_tag="ACG8XC_000486"
     CDS             31569..32258
                     /locus_tag="ACG8XC_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190210.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32666..33352)
                     /locus_tag="ACG8XC_000487"
     CDS             complement(32666..33352)
                     /locus_tag="ACG8XC_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9190211.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(33488..33682)
                     /locus_tag="ACG8XC_000488"
     CDS             complement(33488..33682)
                     /locus_tag="ACG8XC_000488"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190212.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33732..34172)
                     /locus_tag="ACG8XC_000489"
     CDS             complement(33732..34172)
                     /locus_tag="ACG8XC_000489"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFP9190213.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34176..35021)
                     /locus_tag="ACG8XC_000490"
     CDS             complement(34176..35021)
                     /locus_tag="ACG8XC_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFP9190214.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35170..36588
                     /gene="pepV"
                     /locus_tag="ACG8XC_000491"
     CDS             35170..36588
                     /gene="pepV"
                     /locus_tag="ACG8XC_000491"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFP9190215.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36645..37592)
                     /locus_tag="ACG8XC_000492"
     CDS             complement(36645..37592)
                     /locus_tag="ACG8XC_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9190216.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37815..38165)
                     /locus_tag="ACG8XC_000493"
     CDS             complement(37815..38165)
                     /locus_tag="ACG8XC_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP9190217.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38365..39444
                     /gene="pepA"
                     /locus_tag="ACG8XC_000494"
     CDS             38365..39444
                     /gene="pepA"
                     /locus_tag="ACG8XC_000494"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFP9190218.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39588..39908
                     /locus_tag="ACG8XC_000495"
     CDS             39588..39908
                     /locus_tag="ACG8XC_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP9190219.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39930..40394
                     /locus_tag="ACG8XC_000496"
     CDS             39930..40394
                     /locus_tag="ACG8XC_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9190220.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40599..41204
                     /gene="ytpR"
                     /locus_tag="ACG8XC_000497"
     CDS             40599..41204
                     /gene="ytpR"
                     /locus_tag="ACG8XC_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFP9190221.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41243..42532)
                     /locus_tag="ACG8XC_000498"
     CDS             complement(41243..42532)
                     /locus_tag="ACG8XC_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFP9190222.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42960..43439
                     /gene="rlmH"
                     /locus_tag="ACG8XC_000499"
     CDS             42960..43439
                     /gene="rlmH"
                     /locus_tag="ACG8XC_000499"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP9190223.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            43642..43767
                     /locus_tag="ACG8XC_000500"
     CDS             43642..43767
                     /locus_tag="ACG8XC_000500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190224.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            43764..>43937
                     /locus_tag="ACG8XC_000501"
     CDS             43764..>43937
                     /locus_tag="ACG8XC_000501"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFP9190225.1"
                     /translation="MRHSNFLAFLFAPNETHNLSDEFFKMFLKRYIDSNDDTKAAINY
                     FDALLNSYEDLIVY"
BASE COUNT        13932 a         7983 c         9392 g        12630 t
ORIGIN      
        1 atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt acatagaaaa ataaatattg
       61 tatgatatac atgtgaatta tttaacaaat aaccatgacc cattaggagg ataatgatgg
      121 ctaaagtaat agaaaaagaa aaaacgaaga caattgacgt acaagcaatg attgatgatt
      181 tggcagaaaa agcaaatgca gcattgaaag aaatggaaag ctttgatcaa gaaaaagtag
      241 accatatcgt gcatgaaatg gcaatggcag ctttggatca acatatgcca ttagcgaaga
      301 tggctgtaga ggaaacaggc cgcggtgttt acgaagataa agcaatcaaa aatatgtatg
      361 cgtccgaata tatttggcat aatatcaaac atgataaaac agttggtgtg atcaatgaag
      421 atgtccaaaa agggttgatt gaaattgctg aaccagtcgg tgtggtttgc ggagtaactc
      481 caacaacgaa tccgacttcc acaacgattt tcaaatcaat gatcgcgttg aaaacaagaa
      541 atccaatcgt ttttgctttc catccaagtg cccaaaaatc ttctgcagaa gcagcacgag
      601 tggtacgtga tgctgctatc gctgccggag ctcctgaaaa ttgtatccaa tggatcgaac
      661 atccttcgat cgaagcgact tctatgctaa tgaaccatcc aggtatcgcg atcgtcttag
      721 cgacaggtgg agctggaatg gtcaagtctg cttactctac aggaaaacca gctttaggtg
      781 taggtccagg taacgtacca gcttatattg aaaaaacagc aaaaattaaa cgtgcagtga
      841 acgatttgat cgtttcgaaa acatttgata atggaatgat ttgtgcttct gaacaagctg
      901 tgatcgtgga taaagaaatc tatgcagcag taaaagcaga attccaagca caccaagttt
      961 atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc agtgatgaac gaaggaaaat
     1021 atgcggtcaa tccatcaatc gttggacatt ctgcgatgga aatcgcaaaa ttggcaggaa
     1081 tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt ggaaggcgta ggcccagatt
     1141 atccgctatc aagagaaaaa ctttctccag ttcttgccat gatcaaagct aacaacacgg
     1201 accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg aggattagga catacagcag
     1261 ttatccattc agaaaacgaa gagttgcatg tcaaattcgg tcttcgaatg aaagcttgtc
     1321 gtattttagt gaatacacca tctgcagaag gcggcatcgg tgatatctat aacgaaatga
     1381 ttccgtcatt gactttggga tgcggttctt atggtaagaa ctctgtttct cgtaacgtct
     1441 ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg gagaaataac atgcaatggt
     1501 tcaaattacc gccaaaaatc ttctttgaaa agaactctct attgtactta gagaaaatgg
     1561 aaaacgttga acgtgtcatg atcgtttgtg accctggtat ggtccaattc ggttactgcg
     1621 atacagttcg tgaagtattg gcacgacgta aaaatgatgt gaagatcgaa gtcttctcag
     1681 atgttgaacc gaacccatcg acaaataccg tatacgcagg aacgaaactg atggcagact
     1741 tcaagccaga tacagtaatc gctcttggag gcggttctgc aatggacgca gctaagggaa
     1801 tgtggatgtt ctatgaacac ccagacacat ctttctttgg tgcaaaacaa aaattcttgg
     1861 atatccgtaa acggacatat aagatcgaca aaccagaaaa aacacaattc gtatgtatcc
     1921 cgacaacatc agggactggt tctgaagtaa cgccatttgc ggtcatcact gacagtgaaa
     1981 cacatgtgaa atatccgtta gcagattatg cattgacgcc agatgtcgca atcgtcgatc
     2041 ctcaattcgt catgtcagtt ccagcatctg tcacagcaga taccgggatg gatgtgttaa
     2101 cacatgcaat cgaatcctat gtttcagtca tggcttcaga ttatacacgt ggattgagcc
     2161 tacaagcaat caagttagtc tttgactatt tagaaaaatc agtaaaaaca cctgatatgg
     2221 aatcacgtga aaaaatgcac aacgcttcta caatggctgg tatggcgttt gctaacgcat
     2281 tcctaggtat ttgtcactca gtcgcacata aaatcggcgg ggaatacggg atccctcatg
     2341 gacggacaaa tgcgattctt ttaccgcata ttatccgcta taatgcaaaa gatccacaaa
     2401 aacatgcgat gttccctaaa tatgactatt tccgtgcaga tacagattac gcagatatcg
     2461 caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt agtcgaagct ttagcaacgg
     2521 ctgtttatga attaggaaaa tctgtcggca tcaacatgag tctaaaagct caaggagtca
     2581 cacaagaaac actagataca accgtagacc gtatggctga attagcttac gaagaccaat
     2641 gtacgacagc taatccgaaa gaaccattga tctctgaatt gaaacagatc atcattgatg
     2701 cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata agaaaataga tgaaaaagaa
     2761 cttggaaaac gttgtttctc caagttcttt ttttaatttc caagtaactg aaagttactt
     2821 ttgttattta acatttaggt caattttaga cagtttttgt tcaaaaagcg atatttatag
     2881 tggacggtat tactgctata ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa
     2941 agatgattat taaagaagag atttttgtac ctaaagaatt agtgaaagaa aaaattgacc
     3001 tgctcgttga aaacctgaca acgatcaaag acgataatgg tgaatttttg ttggactttg
     3061 atggactgaa agtagatgac aaaagctgga cagtgtggaa ttggccacaa ggtgtcggtc
     3121 tatatggtat ttataaaaat taccagatga caaaaaatcc tcgcgcttat caagtggtca
     3181 atgaatggtt tgaggatcgt atggaagaag gcgctccgcc caaaaacgtc aatacaatgg
     3241 caccgctatt gactatggct tacttgtatg aagacacgaa agattcacgc tatttgcctt
     3301 atttagaaca atgggcagaa tgggtcatga atgacatgcc tcggacaaat gaagacgggt
     3361 tacagcatgc cacatatgga ccggaaaata agaatcagtt gtgggacgat acattgatga
     3421 tgacagtgct tcctttggca aaaatcggga aactgttgaa tcgcccagaa tatttagaag
     3481 aagctcgtta ccagtttatg atccatatca aatacttgat ggataaacga acaggattgt
     3541 ggtatcacgg ctggacgttt gaaggaaatc ataactatgc cgaagcattt tgggctcgtg
     3601 gaaactgctg gttgacgatt gctattccag agatcattga aatcttagaa ttatcgaaag
     3661 aagatgcatt gcgcaaatta ttgatcgaaa cacttgaagc acaagtgcgt gcattaaaaa
     3721 cttatcagtc tgaaagcggt ttatggcata cgttgttgga tgatccttct tcttacgtag
     3781 aatcttctgc aactgcagga tttgcttacg gtattttgaa agcggtccat aaacgttatt
     3841 taccacaaga atataaagaa gtcgcttatc gtgctatcca aggattgctt gaacaaatcg
     3901 atgaaaaagg agaagttcaa aacgtttcaa tcgggacagg aatgggggac agtttagatt
     3961 tttatcggaa tatcggaatc acagcaatgc cttatggaca gtcgttaacg gtgctttgtt
     4021 tgacagaatt gctcgtctct tattgttaaa agaaaggaaa cccatatgaa aaaaacatca
     4081 ctgctgtttg cagcaagttc gcttagccta ggtctgctag gtctaagcgg ctgcggaaat
     4141 aattctagtg tggcaactgt cgcagatgca gatgtaaatg agatagttac cttgcgcttt
     4201 gcttatgcaa gcaacagcca gccagtaatt gatgcaatga atgaatttgg gcgtttagta
     4261 aaagaaaaaa cagatggttc cgtacagatc cagtatttcc cagatggaca actgggtgga
     4321 gaacgtgaac tgatcgaatt gacacagtca ggagcagtag atttcacaaa agtaagtgcc
     4381 tcagcgttgg aaagtttctc taaagattat tcgatttttt cagttcctta tctattcaat
     4441 gatgaagaac atttctttaa ggtgatggat aatgaagaaa taatggagcc aatctaccaa
     4501 tcgaccaaaa atttaggatt taccggattg acctattatg attctggtca gcgcagcttt
     4561 tatatggtag acggtccgat ccatacgccg gaagatttga agggaaagaa gatccgtgtg
     4621 atgcaaagcg aaaccgctat caaaatggta gagcttttag gcggttcgcc cgtacctatg
     4681 ggaagtgatg aagtgtacac gtctcttcaa tccaatttga tcaacggatc agaaaacaat
     4741 gaatttgtct tgtatacagc tggacatggt ggtgtggcta aatactattc ctatgatgag
     4801 catacacgtg tgccagatat catcatcatg aatgatgcca tcaaagaaca gctaactgat
     4861 gagcaggaaa aagccattga agaagcagcg aaagaatcaa cggtttttga aattgaagct
     4921 tttgcaaaag caattgaaga agaaaaagct ttagcacaag aagaacatgg agtccaattc
     4981 aatgatgtag ataatacacc gtttcgagag gcagttgccc cacttcatga tatattcaaa
     5041 aacgatccta cctacaaaga cctttatcaa aagatccaag agcaaggagc gtagctgtga
     5101 tgaaaatgat tttaaacaag gtattagaga tcctgggagc tgttttgctt gttgggatgg
     5161 tcgctgttgt tttatggcaa gtattttcca gaacggtttt aggaaatcca aatacggtca
     5221 cggaagagct tgttcgattc ggtcttgtct ggtttgccat gttgtcttcc gcgtatgttg
     5281 tgggacaaaa agcacattta gccgtaacgg ttttaagtga aaaattaact ggcaaaaaaa
     5341 gtaaaatttt agaagttgtt gttcaaagtt tatttctggt atttgctgcg atgatcatgg
     5401 tgtatggcgg atggaatgct gtcacattga caatggggca gatctcgcct tctttaagta
     5461 ttcctatggg atatgtttat ctgtcagtac ctgtatcggg ggtactcatc attatctata
     5521 gcctgatcaa tctaatcgat acattaagca acaagccagc aattcaagaa aaaatataaa
     5581 aatggaggaa taaaatggca atagaagccg gattagtgtt attttttgta tttgttgtgt
     5641 tgcttgtatt agggatgccg attgctataa gtattgcatt ctcatcgatg atgactttat
     5701 tgttagttat accgtttgat gtttctgctt ttagttcagc tcaaaaaatg gtgggaagta
     5761 tcaatagttt ttcgctgatc gctattccgt ttttcgttct ctcgggcatt atcatgaata
     5821 atggagggat agctaagaaa ctagtggatt ttgcgatgtt gttcgtcggg cgcgtgccgg
     5881 gggctttggc tcatacgaat gtgttaggga atgctttatt tggttccatc tctagctcgg
     5941 ccattgccgc ttctaccgcg attggcggag tgttgatccc tcagcaagtg gaagaaggat
     6001 atgaccggaa gtttgcgaca gcggtcaata tcgcgtcagc tccgacaggt atggtcattc
     6061 ctccaagtac tgcttttatc atgtattcac ttgttgctgg tggtgcttcg atctctgctt
     6121 tgtttatggg aggttatctc gtaggcgctt tgtgggcgct aggaatcatg ctagtagcat
     6181 tcatcgttgc taaaaagaat aaataccctg ttgtcgaaaa aggacagatg gctcatgctg
     6241 gaaaaataat aaaagaggcg attcctagcc ttctattgat tgtcatcatt atcggtggta
     6301 tcttgacggg gatatttaca gcagtagaag cttcagcgat agctgtcatt tactccctgt
     6361 tgattgctat gttttactat aaaacggtaa aactaaaaga tatacctaat atgttgtttc
     6421 aatcagtagc aatgtcaggg acgatcatgt tcttattggc tacttcttcc atgatgtctt
     6481 tcgccatggc gtttaccggt attcctcagg caattagttc gttgattatg ggcgtaacgg
     6541 ataataaatt tattattctg ttattagtca atcttgtgtt attactggtg ggtatgttta
     6601 tggatgtggc gcctgctatc ttgattttta caccaatatt tttaccaatc gtcacaagtg
     6661 taggggttga tcctgtacat tacgggttgt tttcaatcat gaatctgtgt gtaggctcca
     6721 tcacgccgcc agtaggtaca ggactatatg tcggtgcaag tgtaggaggc gtaaaagcag
     6781 aggcaatgct gaaaccgctt cttccttttt atggggttat cttagtcgtc ttgtttttga
     6841 ttacttactt cccgcaaatc gtcatgtggc tacccaatat gctgagttag gagaatagtt
     6901 atgttgaaca ttacaattag aggacatgat ctttccgaag tccaaacgat agaagatctt
     6961 gcagaaaaaa caaaagaaca agggattcat acactgcagc tcgccttagg tctttcgttt
     7021 cctgaaatac cttctggcgc aaatgagatc aattctggga tgggaaatta tgtcaaacga
     7081 gttttagaaa agcaagaagt gaccgtagga attttaagtt gctatatcaa tatgattcat
     7141 ccagatttaa cgatacgaca agagttgctg acaaagttcg agcagtatct tcgctatgct
     7201 tcttcctttg gtgcgtcgat ggttgcttcg gaaactggat gtgttttgcc ggaaatccaa
     7261 tatacggaag aaaatttcac agatgaagca tttgctgaag ctgtgtccgt tatccgtcgc
     7321 ttggtaaaag ctggagaaaa ataccaaatg atggtgggga tagaaccagg tttgaatcat
     7381 ccggtgtatt ctttagcaag ggtagagcag ctgatccaag cagtagattc ggactatcta
     7441 ggaatcattt tagatccgac caatttgatc acaagtagtg attatcaaga acaagtccaa
     7501 ttggtagaag aagcttttga acgttttggt gaaaagatat gtgcggttca tttgaaagat
     7561 tttcgtgtcg aacaggaaaa aatcgtgccg gtaaatttgg gagacggtgt gatcgagtac
     7621 acaaaaatca aagaaatcat catgaagaat cgtccgtacc tgtatgtggt tcttgaagaa
     7681 acaaaagatg atgggattcg ttatggaaga agtcttcttg aataatgtaa agaagacgtt
     7741 ggttaactaa tatttagaat ggagggggcg gggctagaag atgccacgtc tcttcttttt
     7801 tataagagta tataaaagca gctctttttg attacaaaaa acgtggtata ataaaattgt
     7861 gcataagtga acaaaaggag gtgcggcaca tgctccaaaa gaaaatcagc ggtgaacaag
     7921 tcaatacgat ggtttctgat cagtatgaaa tctatcatgt caaagatgtc gtcagccaaa
     7981 ataaaacgat ctatcatcat catgatttct atgagatcca tgctacatta aagggagaag
     8041 cgacttttta tttgaacgga cgacaattta cgatcaaagc aggaagtgtg ctgctcatcc
     8101 attccaatga tttgcatcgt atcgtccgcc aatcttcaga tgtattcgag cgtgtctata
     8161 tctttgtcac cccttctttt ctagaaagtc gttccacgcc atggtctaat ctatcggctt
     8221 gttttcaacc aatgggagag aagaggagtc gtgtattgca aatagaacag gaagtattga
     8281 agcaaaaatt atcatttatc gaccatcctc ctgataaaaa aatttacggt gaagacataa
     8341 aatatgaaca agctctggta gaatacttga tttttctcaa tcaagttgta caaaaagaag
     8401 agagtgcgca agaacagaat gctgtagttc ctaatgagcg tgtggaacaa atgttacaat
     8461 atatctcaaa aaatttatct catccgcttt ctttagagga aatggaaaag cagttttttg
     8521 tcagcaaata ccatatcaca cgagaattta aaaagtacac tggatttacg tttcatcagt
     8581 atgtaatgaa gaaaaagctg atctatgcca agcacttgtt gcgagaatac cgcagctcta
     8641 gtgctgtata tagtaaaagt ggtttttctt cttatccgca ttttctgaaa gcattcaaga
     8701 gagagttcgg cataacgcct aaagaatttt tgaaacgtga tacacagaaa cagttggttc
     8761 attatgaaca ttttgaagat gaacaatgag aatgatcact aaaaaataac cgcagtagct
     8821 gctgttgttt acatagaagc agaaactgcg gttattttat ttttatctat atatttcaaa
     8881 atattttgaa aaaatgacgt acatattata tgaatcagct gaatttacga aagtctctcg
     8941 gcgatagtcc tgtttttgct ttgaacaggc ggctaaagta agcaaaattc tcaatcccta
     9001 tttcttcgga aatcattcga accgtaaatt ctgtataccg aagaaggtct ttagcgtaag
     9061 aaagtttttt gctgatcaaa taatcgattg gtggagaacc gataaatttg gaaaaatctt
     9121 tgctcagctg atatttattc aataagaatt gttgttctaa ttgatcaagt gtaatcgttt
     9181 ctttgaaatg ttcgtctaag tattgcttca gtttgtaaac gtaggcggga atatcctctt
     9241 cttcgaaatc tagctgatac ttttgcaaaa tcagttcatt cagcaactcg tggatagcta
     9301 cagaggactg gaaatcggta cgggcattcg tgccttgttg catgtggatc aattctgtga
     9361 tcaaatggtg gatcttgttt tctgatggat ctcctgttga atggaaggtg ttatggccgt
     9421 cttttgtgaa ctcctgataa aatgattgga cgttctgacc attcaaatgg atccaatcca
     9481 tttcccatgg ttcttcgctg atcgtttgat aacgctgata ttcttggcag tcaataaaaa
     9541 ataagtctcc agcttttagc tgttgctgat tatcattata tgtaagcgaa cctttgccag
     9601 acaaagtaaa ctttatcaag taagaaggta aatgtgctcg ttcagtatag taagggattg
     9661 gtgctttgaa gtaaccgatt tcttggatat aaaaaaataa attcctgctt ctttgtgtag
     9721 gcgtcaagat cactcttttt gagtcttccg accatgagtg gttggctttt tcccagtaat
     9781 ccatgtaaac actcccaact tgtcaagata atgcaattat ataccaaaat agtgtgaagc
     9841 aaaatataag attctgtatt atgatagcat tgtcaataaa tgataacgct ttattaataa
     9901 agtgctaatc aacaataaag taaaagaggt ggagaaatga atccaacatt tgttgccatc
     9961 gatatcggtg catcaagcgg acgactaatg aaaagtacga gggatgcaaa aggttctttg
    10021 atcttggaag aagtacaccg gttcaaaaac ggctttcgga acaaagacgg tcacgatcgc
    10081 tggaagatcg attatcttat caaagagatt ttgattggat ttgaaaaatt aaagcagaca
    10141 gggattgatc gcctttttgc ggggatcgat acatgggcag tcgattactg tctgattgat
    10201 gaacaagggg atcggataaa tgatccgatt gcttatcggg acaagcgtac caatggagca
    10261 gtagaacagt tttctgaaat ctattctcta gataagttgt atgagcagac agggattcag
    10321 atccagcctt tcaatacctt gttccaattg tttgctgaag aaaaagaaaa tttggcaaaa
    10381 gcacataaat tgttactgat acctgattat ttagggtatg tctttactgg taacatggtg
    10441 acggagaaaa ctaatgcatc tactatgcag ctgctgaatg cagggacaaa agaatgggaa
    10501 atagaacttc ttgaaaaaat cggtgtctcg aaggaattat ttccaccatt gacagagccg
    10561 ggaacgatac taggagatct gcaaaaagaa caattttcaa aatatgattt gccagatgtg
    10621 acctttatca cagtagcaag tcatgatacg gcgtcagctg tattaggaac gccaggtcaa
    10681 ggagaacgtt ggggttatat cagcagtggg acatggtcgc ttttagggat tgaaacaaag
    10741 atcgccaatg tttctcgcaa tgctttttct gaaaattata cgaatgaatg gggtgcccat
    10801 aacacggtgc gttttttgaa aaacatcatg gggatgtggt tgatccagga agttgcccga
    10861 atgcaagagt acgagtacag ttatgcggaa ttggcagagc ttgctgcaaa agaaaaaccg
    10921 ctgcagcaag tgatggaaat caatgaccct cgatttttga atcccccaaa tatgatagaa
    10981 gagattcagc aatactgttt tgaaacagag cagccagtcc ctgtctcatc gggagctatc
    11041 gctcgttgtg tgtatgataa tcttgctctt tgttacgcac aagagctgat caaagtagaa
    11101 cacttaactg gaacgaaatt ggaaaagctg cacatcgtag ggggcggatc gaataatcgt
    11161 ctactgaatc aattgacagc agattatgcc ggaatcattg tagaagcagg acctagtgaa
    11221 gccacagcaa tcgggaatgt tgccatgcaa atgctcagta caggtcagtt ttcgaatata
    11281 gaacaagcaa gaaatgtaat caaacagtcg tttccatgtg aaacttttta cccagatgaa
    11341 aataagcaaa acacgatgga atggtacgtg aataaaatca aaggagtaat cgtatgaaca
    11401 cgattgaaag aaattacgaa caagcaaaag aaaaatatgc aactatcggt gtagatacag
    11461 atgctgtact agaaaagata caggacatca aaatttctat gcattgctgg caaggagatg
    11521 atgtcaaagg tttcttgaca ccggatggcg aattgaccgg tggaatcatg gcgacaggga
    11581 attttccagg agccgcacga acacctgaag agcttagaca agatctggag aaggcttatt
    11641 cgttgatacc gggcaaacac aaattgaacc ttcatgctat ctatttggat acagaagaat
    11701 ctgtcgatct aaatgaaatt gagccgaaac attttgaaaa atgggtagag tgggcaaaaa
    11761 aagaagaaat cggtcttgac ttcaatccga ctttcttttc gcatccaatg atgaaagacg
    11821 gctttacatt ggctcatcca gataaagaag ttcgtgaata ctggatcgaa cacgggaaaa
    11881 gaagccgcaa aatcgcggag tactttggga aagaaactgg gcaaacaagt atcaataact
    11941 tttgggtacc tgatgggatg aaagataatc cgattgatcg ttttacccca cgggaacgtt
    12001 taatggaatc actcgatgaa atttttacag aagagttaga tgaacggtat gtacaagatg
    12061 cagtagaaag caaactattt ggtttaggcg ctgaagctta tacagtcggt tcccatgaat
    12121 tttatatggg ttacgggttg actcgtgaca aacttgtttg tctagatgct ggacattttc
    12181 atccaacaga agtgatttcc aataaattat cttcattagc tttattcagc aaaggaatca
    12241 tgttgcatgt ttcccgtcca gtgcgttggg attcagacca tgtggttttg atggatgatg
    12301 aattgcagga aatcgctaaa gaattagtcc gtaacgaatt gttggaaaaa acaaatatcg
    12361 gtctggattt ctttgatgca acgatcaacc gtatcgctgc ttgggtcatc ggtacgagaa
    12421 atacacaaaa agcactgctg aaagctatgc tggaaccagt tgagcgattg aaagaaatgg
    12481 agctggcttt tgactttact tcaagaatgg cttatacgga agaattgaaa gatttcccgt
    12541 atgccgatgt atggaattat ttctgttaca aaaatcaagt tccagttggt ttggattggc
    12601 tggaagaagt ccaggaatat caaaaagacg tgttggaatt aagaaaatag gagtgtggct
    12661 atattgaaaa aaatagatat tttacaagcg ccttatgtga aagaaatgat cgaaacgacg
    12721 tcgaatcttt atcgcttggg atgggatgaa agaaatggcg ggaatatttc ttatttacta
    12781 aaagaagaag agatcacacc gttcttggat cctgaaaaag tgatccgccg catctcgatg
    12841 atttttgatg ccagccgttt agcaggatgc tattttattg taactggttc aggaagatat
    12901 tttaaaaatg tagcgagcga acctgccgag aatcttggaa tcgttcgagt tgctgaagat
    12961 ggcaaaacac tggaactttt gtgggggctg gaaaacgaat gccttcctac aagcgagttg
    13021 ccaagtcatt tcatgagtca tatcgcccgt ttagaagtag acccagataa tcggatcgtt
    13081 atgcattgcc atgctagtca tctattagcg atgagtttta cgcatgaatt agacgaacgt
    13141 tcctttagtc gtacgctatg gcaaatgtgt acggaatgct tagttgtttt tccagaagga
    13201 atttccatca ttccgtggat ggttccggga acaaatgaga ttggggaagc aacagctgaa
    13261 aaaatgaaag aaactcgatt ggttttatgg ccacaacatg gaatctacgg gtcaggaaaa
    13321 gacatggatg aagtctttgg tttgatcgag acagcagaaa aggcagcaga agtctatacg
    13381 tacgtcaaag cgcaaggacc aatcttgcaa acaatcacgg acgaaaacct gtggcattta
    13441 gcagatgcgt ttggtgtcac tccaaaagca ggttatttgg aggaagttca tacgaaagca
    13501 ggtgtctgac atggtgaaaa aagctttttg catgaaagtt tacccagaaa aacaagaaga
    13561 atacaaaaaa agacatgatg aactttggcc agaaatgcgt acagcacttc gtgaacacgg
    13621 ggtcatcgac tattcgatct atcttgatcc gagtacaagt ttgttatttg gttacttagt
    13681 gatcgaagat gaaaagttat gggaaaaaat ggcaaataca tctatcaatc aaaaatggtg
    13741 gaagtacatg gaagatatca tggagacaaa tccagatgcc tctccagtca gttgggaatt
    13801 gatgccagtc tttgaactgt agcaacaagc aactgggcaa ccggttgctt ggggttcttt
    13861 cgtttttatt gtgaaaaata atacaaatta ggaggaatag aaaatgagta atcgaatgat
    13921 tttaaatgaa acatcgtatt ttggaaaagg agcgattgag tcgatcgtcc ctgaatttca
    13981 gcagcgagga ttcggcaaag cagctgtcat tactgacaaa ggattgatcg agcacggtat
    14041 agcaacaaaa gtaacagaac ttctagataa agcagcaatc gcttatgctt tatacgacgg
    14101 gatcgtacct aatccaacga tccaaaatgt aaaagatgga gtggcttttg ttaaagaggc
    14161 agaggcagac tgtttgatcg caatcggtgg aggttcgcca atcgatacgg caaaagccat
    14221 tgggattatt ttgacgaatc cagagttttc agacgtcatt agtttagaag gtgttgctga
    14281 tacgaaaaat ccatgtttac ctatattagc tataccaacg acttctggaa cagcagcaga
    14341 agtaacgatc aactacgtca tcacagatga agtaaatcac cgaaaatttg tttgtgttga
    14401 tcctcatgat atcccaatcg tcgcgttcat cgacagcgat atgatgatgg gaatgccgaa
    14461 aaaattggct gcatcaacag gtatggatgc tatgacgcat gctattgaag ggtttatcac
    14521 aaaaggcgct tgggaaatga ctgatatgct gcatttaaaa gcaatcgaaa tcatcggcca
    14581 ttctttggaa gcttctgtag atggcgatca aaatgggcga gaaaaaatgg cattaggaca
    14641 gtatattgcg ggaatgggct tctcaaatgt cggtttagga ctggtacacg ggatggctca
    14701 cccgttaagt gcatggtaca acattcctca tggcgtagcg tgtgcagcac ttttgccaac
    14761 tgtcatgaaa tataacaaag aatatactgg agaaaagtac cgtgagatcg ctcttgttct
    14821 agggattaaa ggagcagctg aaatgtcttt agaagatgta agagaagcgg cttgtggtga
    14881 gatcgatcgg ttgagcaaag cggttggaat cccagaaacc atttctgaat taggtgtaaa
    14941 agaagcagat attccagcaa ttgcagaaga tgctttgcga gatgtatgca caccaggaaa
    15001 tccaagagaa acgacagtag aagaaatcat tgctttatat caaagtttga tgtaaaaacg
    15061 aagcggatct tcaacattaa attgcctgta aagagaaatg gtagtttata tctggatatc
    15121 tcttttaaat tgatggtatt tccgctataa tttttcttat aaagaaatag gagaatatta
    15181 tatggaaaat aaaaagcctt atggaactgt attgatcaaa gcctctaaaa tactagacta
    15241 tttagcggaa aaccaagatg tctctttgca gacgatcgca aagggtgtgg gaatgacctc
    15301 ttctacagtc ttgaaaatac tagatacttt gttgatgatc ggctacgtaa ataaaaattc
    15361 agagaaaaac tatcgtttag gtgcaaagct tattcgctac gcgaataaaa acattgaaca
    15421 aatcgatttg gtagagttga ctttaccgtt tttagaacag ctgcagcaaa agatcgatga
    15481 gactatccac ttaggggtat tggacaacaa tgagatcttg tacgtgaaca aactagatcc
    15541 aaaacatcaa acgatccgca tgtcttcaaa aatcggaatc acccgaccat tatacaattc
    15601 tgcgatgggc aaagccgtat tagcagaatt cagtgaagag cagtacactg attatcttga
    15661 aaagcatccg ttgatccctt acacagaata tacgatcacc aatgcattac ggttgaaaaa
    15721 ggaaatcaaa acggtaaaag aaacccacat cgcatttgac gatgaagaaa tcgaacgtga
    15781 tattttttgt ataggagcct caatcgtgaa aaacgaacaa atcattggtg catttagtgt
    15841 aagtatgccg aaatatcgtt taacagatga gacaaaagaa caaataatca gtgccctcaa
    15901 gcaaacaaaa agcgaaatcg agcaaagaat tcaaaaataa tgaaaaaagc gtttgcaatt
    15961 tttcttttct gccgtataat atgggtatag atttccaagt tataaaataa aatttctaaa
    16021 taggaaatag gtggagaaga tgaaaaaaat tgatatttta tctcgattga aaaacgccgg
    16081 agtgattgcg gtggtacgcg gaaaaagcaa agaagaagct ttgaatgctt gccacgcaat
    16141 catcaaaggt ggtctaactg gtattgagtt gacttttaca gtccctcaag cagatcaagt
    16201 aatcaaagaa cttttgtcat tctacaaaga ccaaccagaa atagtgattg gcgcaggcac
    16261 agtattagac gctgtcacag ctcgcttggc tatcttagct ggcgcagagt atattgttag
    16321 cccttccttt gatcaagaaa cggcagagat gtgcaatctg tatcaaattc cttacttacc
    16381 aggatgtatg actattaccg agatcaaaac agcgctgaaa agcggggtag atatcgtcaa
    16441 actatttcct ggaagcgctt atggaccaag tatcatttct gccttcaagg cgccgatgcc
    16501 tcaagtcaat atcatgccaa ctggcggtgt gagtctggac aatatgaaag agtggttcga
    16561 tgctggtgtc gtaacagtcg gagtaggtgg gaacctttta gcacccgcag ctacaggaga
    16621 ttttgacaaa gtaacggaag tcgcgcaaca gtatgcagca aaaatgaaag aaatcaagag
    16681 gtagctgctt atgggaaaag tagtgacact cggagaaatc atgttgcgat tgtccacaga
    16741 gcagggaacc cgtatcgatt caagtgaacg attcgctgcc cattatggcg gaggggaagc
    16801 aaacgtagca atttctttag ctaattacgg acatgaagcg atttttgcta caaaagtacc
    16861 tgataacgca ttgggaaaag cagtcaaaaa gcacttgcaa tgttatggag tagatacgaa
    16921 acaggtatta tctggtggat caagactagg tacttattat atggaaactg gtagtggcga
    16981 acgcgccgcc tcagtgatct atgatcgtgc cggatcaagt tttgctgaaa tggatcattt
    17041 ggaatggtcg ctagaggagt tattccaagg aatagatata tttcatgttt caggaatcac
    17101 cccagctttg tctaaaaaat ggcaaaaaat gacgaaacag gtgatagaag ctgcaaaaaa
    17161 agctggctgc ctgatcagct tcgatatcaa ctatcgtgga aaattatgga cacagcaaga
    17221 agccagcgaa gtaatccatc agcttttacc tttagtagat atctgctcag ctggaaaaat
    17281 ggatgctctt tatttgttag gtatcgagaa agcgccagaa gcagaagaac aacctctaat
    17341 ttattattat caaaaaatgc aggaaaagtt cccgaatatt caggtgtttt attcgaccaa
    17401 gcgtaccgtt cattcagcaa gtgaaaatga attgaagggg acattgtgga tgaaccatga
    17461 atatgtggaa tcccaagtcc atagaatcac cccaatcgtg gatcgagtag gtggcggaga
    17521 tgcttttgca ggtggcgtgc tccatgggat tttatccaat atgtctccgc aagaaatcat
    17581 tgattttgct acggcagctt ctgccttgaa gcatacgatc catggggact gcaaccaatt
    17641 tagtcaagaa gaagtcgcac aatttttagc atgcggctct ggaaaaatca ttcgatagaa
    17701 ggagtagaag aaaatgcaag aaatggaaac acgttataca cacagtccag aagatatccg
    17761 tcattattca acggaacaat tgagaaaaga atttttagta gaaaaggtat ttgaaccagg
    17821 gaaaatcagt ttgacttata cgcacaacga tcgaatgatc tttggtggag tgacacctac
    17881 tgaaaaagaa ttagaaatca cattagacaa agaactaggt gttgattact tcttagagcg
    17941 tcgtgaacta ggtgtgatca atatcggtgg accaggattc atcgagatcg atgggcaaaa
    18001 agaagaaatg aaaaaacaag acggatacta tatcggaaaa gaaacacgac atgttgtatt
    18061 ttcttctcaa gatgctgaaa acccagctaa attctatatt agttctgttc cagctcatca
    18121 taaatatcca aatgtaaaaa tcagtatcga ccaagtaaaa ccaatggaga ctggtgaagg
    18181 cctgacactg aatgaacgta aaatctacca atacatccat ccaaatgtat gcgaaagctg
    18241 tcaattacaa atgggttata cgattttaga accaggaagc gcttggaata cgatgccttg
    18301 tcatacacat gaacgtcgta tggaagctta tgtctacttt gattatgcca atgaagatac
    18361 acgtgtgttc cacatgatgg gcaaaccaga tgaaacaaaa catttagttg tagacaatga
    18421 acaagccatt atttcgccaa gctggtcgat ccattcaggt gtaggtacaa gcaattactc
    18481 attcatctgg gcaatgtgcg gagaaaatat cacctataca gacatggata tggtgcaaat
    18541 ggatcaatta aaataagcat aagggagaga aaatgatgga atttagtatg gatatgtttc
    18601 gtttagatgg aaaagtagca ttagtcaccg gtgctgtcta tggtattgga tttgagattg
    18661 cacgctcttt agctacagca ggagcaaaaa tcgtatttaa taacttgaat caagaatcag
    18721 ttgatgaagg tatagcgcat tataaagaag caggcatcga tgcaaaaggt tatgtctgcg
    18781 atgtgaccga tgaagaagcg gttcaagcga tggttcaaca gatcaaagaa gatgtaggtt
    18841 cagttgatat tctagtcaac aatgcaggga tcatcaaacg gacaccgatg atcgaaatgg
    18901 atgcggcaga cttccgccaa gtaatcgacg tggatttaaa tgccccattc atcgtttcaa
    18961 aagcagttat tccggatatg atcgaaaaag gcggcggtaa aatcatcaat atctgctcca
    19021 tgatgagtga attaggacgc gaaacggtca gtgcttatgc tgcggcaaaa ggcggattaa
    19081 aaatgctgac gaaaaacatc gcttctgaat acggtcaata caatatccaa tgtaatggaa
    19141 ttggaccagg ttatattgct acaccgcaaa ctgctccatt acgcgaaaca caagaaaacg
    19201 gtgaacgtca tccattcgat caatttatcg ttggacgtac gccagcagca cgctggggcg
    19261 agccaatcga tttggctgga ccatctgtct tcttggcttc tagcgcatct gatttcgtta
    19321 acggccatgt cttgtatgtg gacggaggca tacttgccta tatcggaaaa caaccttaaa
    19381 agagctttag tacactgttt agtggagagg ttgtgaaatc agcggattga acgaataccg
    19441 aaggacagct gattaaacat catttattcg aaaaaagagg ctgggacaga agcatttaac
    19501 tccaagaaat aagaaggaat tcacgaaaat tgcttttcaa atttttgtga atttcagctt
    19561 atttccgaag gagttgcttt tacttccgcc gtttgtacgt gtttagagcg tgagacaaaa
    19621 gtgttcttta cttttgtccc acgctctttt ctgtgcgtct tccatacttc actaagtcga
    19681 tccctcttgc ttcgcaccaa tccttcactg gtccggaaaa gatcagagat tgttggtgta
    19741 gggccgcggt attcgttgcg ccgaccatag tataaagcag acgaagctct tcttgccatt
    19801 gcttcatcag tataatagtt tcttctacgc cgtgattcat cagatgcgtc agtacggttc
    19861 ctgaagtacc cacggcattt gcgccgagac aaagggcttt aaatatgtca taggcattgc
    19921 ggatacctcc ggaggctagg atttccatag aagtgtctgc ttcatttgct tcaagcaatg
    19981 aagagactgt cgattgcccc cagtcagcta aataactcag ttcccgcttg gagcgacggg
    20041 cgttttcgat ctgggtgaag cttgttccgc ttcgaccgct gatatcaact gtatgaactc
    20101 ctaaagcagc tagatcattg agcgtttctc ttgtcatgcc aaaaccgact tcttttacga
    20161 tgagggggac gtcaatggct gtttgagttt cttgaataag tactttccaa ttggtaaaat
    20221 cacggtctcc ctcaggcata accaactctt gaggtgcatt caaatgaatc tgtaaggcat
    20281 ctgcgtgaaa caatcggata gcttctttgg ctctttcgac agaagtccct gcaccgatgt
    20341 tggcaatgat tttcccgtga ggatattctt gtcgcataat cgtataagta tcggctaaag
    20401 aagggacttt caaggcagcg ctgactgaac cagtggcaat catcaaatct gcttctcttg
    20461 cgacgattgc aagatcttgg ttgatttttt ttgttttttc gcttccgcca gtcatcgcat
    20521 tgatataaaa tgggctgctt agtgtaaacc caactgcctg tgtacttaga tcgacatcag
    20581 aaactgctat ttgcggcaaa gggttatgga tgatccgtac gaagtcaaat tcgttttttt
    20641 gtttatcatg aaaggcttta gctagtgaaa catgttcgtc ttttcgattc atcggttttc
    20701 ctttctttga tcgctataga catgtaacgg cagtggagtg atttctgctt tttcccatgc
    20761 actcattaaa ggaagaatgc cagatttctg gtcaacaatt acgattccgc aatcgccccc
    20821 acctgcacca gaagattttg cggctccttc atactgttca gctaaattac acaatttgtt
    20881 caaagcaggc gtttcgatga cgaccccagt gattgcagat aaatcatgca gtaattgtcg
    20941 gttttttcga atcatctgtt ggatcaacgt tacattattt tctttaaacc ctttgatcat
    21001 ttcgttgaca cattctgtac tattttttaa gaaaagctga taagccacca ttttatcttc
    21061 tctcgaacgg tgaacttgat cgaccaaatc agaagtagag gcagggctac ccgtccaacc
    21121 aatcaataaa cgtaaatctt caggagcaat caatggctca atggatagac ctggccaatc
    21181 cagggcaagt aactcactga tagaatgctg agtttcttgt tcttggagcc aaggatggtc
    21241 gaaggttgag aaagcgatcc agccaccata acagctagca gcgatgtcgc cgcaggaacc
    21301 attatcttga actgctaaat tggctagtgc tgcaatcttg aaaatctcca actgagataa
    21361 attcaacgcg taaaaaacat tcaaggcttt gaccgttgca acggtcacgg caccgctaga
    21421 acccaatcca tatttccggc catttgaact gtctaactca cttgttactt tcagatcata
    21481 aaaggataaa agaatgtttt tttcttgtgc atacttttca gtcaagcgaa tagcagcaag
    21541 gatataatga aaaggatttt cccgaatatc taaaactaat tctccgttgc gtcttgtcca
    21601 acgtacaggc atcccactat attgtgcaga ttggatactt ccgacttttc gtgcagattc
    21661 tacagtgact gtcacgaatt gatcgactgc agcgataact gctggatggc ctgtttcaac
    21721 aactgcatat tctccggcaa tatagagttt gcctggtgca gatacttcaa tcataaaaat
    21781 ctccctttat tcaataatcg caattcctgg accagcatgt gcagtaatta cttgttcttt
    21841 gctaaataat ttggtaaatg tttcttgtac ctcagataaa tggctgtttt ccactaaaac
    21901 tttgacatta gggccagcat ccatcgtaaa gtagcatgga atgccttgct ttctcaattg
    21961 tcgcaccgca tccatcgctt ttaagctgtc aggagaccag taagtgaatg gaggctgggc
    22021 agctaaagtc gttccatgca tttttagtcc gtttctttcc atcgtttctc cgagaagttg
    22081 gaaatctttt gttttgattg cttgtttcaa ttgatataga tccccttcaa cggaatctaa
    22141 ccagccttga taaaaactgg atgtttcgac tgtccgacgc atcccatttc tgctggacac
    22201 ttcttttttc tggtcgttga tcaaaacgaa aaccattgct aaatcgtctt cgaaagaatc
    22261 ggaagggact ggcttagcgt aagaacttaa gtcgtcatgg cctttttccc attcgacgaa
    22321 accaccgaaa atacttcggc aagctgaacc agacccgcgt cgagcaaatc ttgaaagaga
    22381 gagatcatct aatccaagct taagagcagt attgcaagcg cctgctaaag cagctagccc
    22441 gctggcagag gaggctaatc cagctgcggt aggaacgaaa ttctgactga ttactgaagc
    22501 ttttagtgag aggccggctt ctttacgaaa aaggtcaaga aactgactga ctttttttgt
    22561 tgctttttcg tcttgcaagg tgccatctag ataaaattca tctaccatat agctatcaga
    22621 aaagatgact tctgtctctg tataaaatgc atccaatgtt aacgaaaggc tgttgttcat
    22681 tggaaggatg agttcttcat ttttcttacc ccaatatttg attagagcaa tgttggtata
    22741 tgcgcgtgct ttgcctttaa acataggtat gtactcctaa tccttgtatc caagtttcag
    22801 cagcacccgc atcttcaagt gcttgactga tttcttgggc ttttgttttt gtttgtgcta
    22861 atgcaatcat acagccaccg cgtccaccgc cagttaattt ggcacccaat gccccggtat
    22921 cttttgctgt ttggatcaat agattaagaa aatcattgct gattgtcagc ttttccagaa
    22981 gtgattgtga ttcatccatt gtctgtgcca acgtttctgg tgaattttca atgattgctt
    23041 gttttgcttg cttcgtcaag tatcctaatt tttggatcat ttgtccagtt tcatgaggct
    23101 gggtttcaaa gaggtgagcg acatctttga cggcttctct tgtttgtcct ttgattcctg
    23161 tatcagcgac aatcaaaaaa gcatcgatgt tcaaagaaaa gtagtcgaaa ggatgccctt
    23221 ttgtaaaata aatcggctgc aagctgctag tagcggctgc atcgattcca ctaggattac
    23281 cgtgggcgat tttttccgaa agctggacat tttctaatag tatttcacga gacaaaggaa
    23341 atgctaagta atcataaaaa gcacgagtga ctgctgtggc gactgcagcg cttgatccca
    23401 ttccacgttc agcaggaatc gtgctttcaa tggtcagttg caagtttttt tggatgtcat
    23461 gctgtttttt taagtgaatg aataattttt tgatattttt taatgcatga ggagcttcgg
    23521 ctaaatttcc tgaatagtag gaagaaacca gttgatcgtc cattgcatcc agctcttcaa
    23581 ggaatgcggt gacttttgtt gcatagaaag gaaaggcaat cgctggttct ccataaacaa
    23641 ccgcgtgctc gcccatcaat atgatctttc cgcttgattc tccttggcca tagtttgcca
    23701 taatctaact ccttctcacg taaaagtgat ttttaagcaa aaaataaagc gcccaaaacg
    23761 agttgcgctg cttcacgaat ttttttgtcc actaagggtc tcaaaaaaca ttcctccttt
    23821 attttactaa aaccatctca gaagtaaaac taagaatacg ttttctaaag aaaaaacact
    23881 tttttcttaa gaattttttg actttttttc ttctttgact agaaaaaaat acaattgaca
    23941 ctactagata ttgggggtat atttaaaaag gttggagtta aacacaatat ttagtgatga
    24001 agtgagctgg aggtaaatga gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc
    24061 aagtttaaca gaaatgatgg ctgaacactt tgatagtcag gcattttatg aatcgattga
    24121 tgataatgaa gtcttgccct tattttatgc aaaccctgaa cagtatgctt ttttgctgca
    24181 aatttatttt ttgaataaac gttttgcaag catcaaacaa gcaatgaaag atgacaataa
    24241 tgtattggac cgttcgattt atgaagattc actgcttttc catctaaatg cagatctagg
    24301 aagagcaaca gaaacagaag ttcgtgtgta tgatgaactg ctggaaaaca tgatggaaga
    24361 acttccctat gctgcccata aaaaacatcc ggatttatta gttcatattc gtgtatcttt
    24421 tgatacgatg ttggagcgta tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga
    24481 tccaagtcta tacgattatt acaaagaatt gaacagacgt tacgatcaat ggtatgaaga
    24541 atacaaagaa agtccaaaaa tccaaatcga tggagaccgc tataattttg tggaagatcc
    24601 tcaagcaaaa gaagaagtct tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac
    24661 caaagtagcc atttgattta cagttgctca aataaagggt aaacctttat ttgagcgact
    24721 tttttcttta tactggaaac aaatgtcagg aggatagagt atgcgggaaa aacactattt
    24781 taatcaagac cgtaattttt ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa
    24841 cttaaaagaa gaatatggca ttagtgacga gatgctgact tattctctag ataaaaatga
    24901 acgggcgcgc gtagagtacg attcttttga tgaagcgtta ctgcttgtat caaatgttcc
    24961 gcaccagcaa aaagtcgata atcattatga aactagtcct atcgctttta ttttaaaaga
    25021 agacggtttg tttacattta ccacgccgaa taccgagtac gtgattcgac taattcgatc
    25081 gcttttggaa cgaatgccag acatgagtgt atacagttta ttgtttcgta cattgttttt
    25141 gatttctgat tccttttttc ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa
    25201 tctgaaatta agagaaaaaa caacgaacaa gaatctgctg caactttcag atttagagat
    25261 cggtttggtt tacttagtta caggaacaaa acaaaatgta gtacttttag aacaaatcaa
    25321 ggcattggct atttatcgca agttgtcaga aaaggaaaaa gaagagttgg acgatgcgct
    25381 gatcgaagca cgacaagcag ttgaaatgac gaacttagct tcgcagattt tagaccagct
    25441 ctctggcact tataataatt tgctgaataa caacttgaat gacacgatga aatttttgac
    25501 ggtctggtcg ctgatcttga ccgttccaac tattgtgaca ggattcttcg gaatgaatct
    25561 ccagttaccc tttacccatt ccgttttcgg atggggaatc gccttgatca tcagtttggt
    25621 cttatctatt tggatgctta ttgcactgtg gcggcggatt cgttgatcta gtctttgatc
    25681 tgcaaaccaa gggcttgggc gatcacaagt gcttgctctt ggttgacgat tagtcccgcc
    25741 aaagaatctt gagataaagc gattgctgtg aagaaattag tcgtaaagtc taatcccttc
    25801 agagaagttc gaaaccagtt cgaaccggtc aactgattgt cagacaaaaa aaggtttttc
    25861 caagtgacct cataaaattc gctgtcttct aattgacaat gatcgaagga gacagctttc
    25921 aagtttgtac tactaaagga agccatcgaa gcaatacaat cagtgaaagt acagtctcga
    25981 agataacttt cggcaaaatt cgtacctgtt aatttacatt gatgaaacac gacttggtgg
    26041 aaactgccac caatccactc cagattggaa aaatcacatt tttcaaaaat gacattactg
    26101 cattcaaaat gacgtaaaac tgttttttgc atcgtaagtt tttggatatg actctctctt
    26161 aataccatat ttttggcatc gagatagctt tggtcacccc cgttgatcag aagaccagag
    26221 aagatctcct catcttctaa ataaaactct ccctttgtaa gaagaggaag agaaggtgga
    26281 ataggttcga tttttttcat gtgggttcac ctcgtttttc tttagtatag caaagaagcg
    26341 ggaatcactg ctagaaagtc tgctaatcta atcattttca gaaaaggaag acaaactgta
    26401 tgaaaaacat tgacaaaaag attagttttt gctatactat gatggttgag aaataaaagc
    26461 agaagcaccc gcttctcgcc ttagttgata atgtcactgg gctagatagt tttcactggt
    26521 tctattaggt gaagtacgcg cgggttctgt aagagaactc gtttttttat tgccgtttat
    26581 tcggtaagct tttctttctc tcataatatt tggaggtgaa tgaccatagc aaaggatatg
    26641 atggttaacg acggcattcg tgcacgagag ttacgattga tcggttcaga tggtgagcaa
    26701 ttaggagtta agacaaaagc agaagcattg aatattgcag aacaagcaaa cttggatctt
    26761 gtgttagttg cccctggtgc gaaaccacca gttgcgcgaa tcatggacta cggtaaatac
    26821 cgtttcgaac aacaaaagaa agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta
    26881 aaagaagttc gcttaagtcc tacgatcgac ttgaatgact tcaatacaaa acttcgtaat
    26941 gcacgtaagt tcttggaaaa gggcgataaa gtgaaagctt ctatccgttt caaaggccgt
    27001 gccattaccc ataaagaaat tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg
    27061 gatattgcaa cagtggaaca aaaagcgaaa atggacggac gcagcatgtt cttgacgctg
    27121 gcaccgaaaa acgacagtaa gtaatttgaa tgactaactg gctgttggac agccgatagt
    27181 cggataacga aagattttga ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg
    27241 gatcagcaaa acgtttcaaa cgtactggta aaggcggatt gaaacgcttc cgcgcgttta
    27301 caagtcaccg tttccacggc aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca
    27361 tggtttctag tggcgacttc aaacgtattc gccaacaact agcaaaaatg aaataagaag
    27421 tacaaataac agaatctgaa gatttatttt tattaggagg aattatacat ggcacgtgtt
    27481 aaaggtggaa cagtaactcg taaacgtcgt aaaagagtgc taaaattagc aaaaggttac
    27541 tacggttcaa aacacacttt atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac
    27601 tacgcatatc gcgatcgtcg tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt
    27661 atcaacgcag cagctcgtat gaatggcttg agctactcaa aattaatgca cggtttgaaa
    27721 gttgctgaaa tcgacatcaa ccgtaaaatg ttagctgact tggctgttaa cgatgcagca
    27781 gcatttactg cattggctga ccaagcaaaa gaagctttgg ctaaataagc taaacaatta
    27841 taaagaggaa agaagataac gaaaagttat gttctttcct ctttttttat ttacaatcct
    27901 ccatcaaatg aaacaggaca ataacgtata agtctaaaat agcataatta aggtttttta
    27961 attgaaaact gaacatttat ggtcaccctt taatgaaagc gctaactaca taagttagaa
    28021 tgagtttgac tgatatatta gtttgcttat cagaaaaaca aaatggacgg aggaataaca
    28081 tgagtgatat tattattggt attttgttag ttatctcatt ctttttcatg gtctggtact
    28141 gtgtaaaagg ctacaacttg atggtcggct ttgccatcat ggctactgta tggatgggat
    28201 tagcgctagt cggaaacacg ttttcaccaa acccggcgat ggaagggcaa ggagtaattg
    28261 atgtgttgac gcatatctat acgacggggc cagctgagta cgccaaatcg atcttggtaa
    28321 atgtcttctt tggtgccttt ttcgggcgtg ttctagtaga tagcggaatc gcagctaccc
    28381 tgattcgaaa ggtagttgaa cttggagggg acaagccaag aatcacgatg agcttactat
    28441 gtattgtaac cgctgttata ttcatgtcga tgacaggaat cggaccagtg atttctattg
    28501 cagttattgt gttgccaatc ttgatgtcat taggtatttc agtaccggtt gcactgttct
    28561 catttatggg ctcgattatg gctggaatct ttgctaatat cgtcaatttc aaacaatatc
    28621 aaacgattta tgctggtttc aatccagcgg ctgaaagcta tacttataat gactatttcc
    28681 aaattggtat gattgggatg attgtaagct tagttgtggt actaacggtc gctaatatat
    28741 caatgaacaa aaagaaacgc tatgcaatgg cagcaaacgt accagctgaa ggtggcgacg
    28801 caccaatgat ctcatggtta gccgtattat taccagtttt aggggtcgtt cttttagatc
    28861 ttccaatcat cttaggtttt attctagcag gtatctgggc attgctgttt actggaaaat
    28921 tgcgcggagg ttataaagaa atctgtcgtc agtttgcgaa attatttaca gatggcgcag
    28981 tagatgttgc gccaatggtt ggtttcttga tgaccttagc gatgttcaat aattcagctg
    29041 cgtatgcttc tccttatttt tcagctattt ttggagattg gataccgcaa tcaccattag
    29101 tcttggcaat cgtatttgct atattgacac cactaggatt tttccgcggt ccaatgaatc
    29161 tagtcggttc aggctcagct attttagctg tcgtattagc tgttaatcca acgatgtcgc
    29221 cagccttttt gttcccgtta tttgcaatca caacgattgc tcctcagcat ttagatatta
    29281 cacaatcttg ggttgcttgg ggattaggtt atacaaaagt aacttctcga gaatatatga
    29341 agaaatccat cccaactggt tggatcatcg gagcgatctt atgcctgatc acgttcttat
    29401 tgtacgggaa cgcctagtaa acaaaacgtc gataaaaacg attagatgaa tggaggaaaa
    29461 gaaaatgaaa cgttttgtgc gaatgggaat tgatgttggc ggtacacata cgaaagcagt
    29521 cgcaatcgat aatgcgacgc acgaaatcat cggaaaatct tcagtgaaaa caacccacga
    29581 tgatgtccgt ggagtagccg caggtgtcgt tcagtctttt caaaattgtt tgctggaaaa
    29641 taatatcagt ccagaagatg ttgtgtttgt tgcacattct accacacaag cgactaatgc
    29701 actgattgaa ggagacgtag caaaagtcgg cgtaatcggg atggcaaaag gtggattaga
    29761 aggtttttta gcaaaacgac aaactagatt gaacgatatc gatttaggga ataagaaaaa
    29821 aatagagatt gtcaatgcat tcttacctgt caaacactta aatgtagacc gtgtttcaga
    29881 aacgatttct tctttagaaa gagaaagagc ggaggtcttg gtttcttcta tggcatttgg
    29941 agtagataat ggggaaccag aacgagttgt ttatgaagca gcatcggtaa aatcgatccc
    30001 tacaacgatg gcttctgaca tcacaaaatt atatggattg acaagaagaa ctcggacagc
    30061 agcaatcaat gcatctattt taccaaaaat gctagatacg gcaacctcta cagaagattc
    30121 cgtacgagaa gcaggggtca atgtttcttt gatgattatg cgcggagacg gcggagtaat
    30181 ggaaatcaat gaaatgaaaa aacgacctgt tttaaccatg ctttccggtc cagcagcatc
    30241 agtcatgggc tctttgatgt acttgagagc gtctaatggt gtttattttg aagtaggcgg
    30301 aacaacaacc aatatcgggg tcattaaaaa tggacgtcca gcaatcgatt attcaattgt
    30361 aggtggccac ccaacatata tctcttcatt agatgttcgg gttctaggtg tggcaggcgg
    30421 ttcgatggta cgggctaatc agtcaggaat catcgatgtc ggtccacgtt ctgcacatat
    30481 tgccggtcta gattatgcag tatttacgga gacagaaaaa atcaaaggtc ctaaggttga
    30541 atttttctct cctaaagaag gagaccctgc tgattacgtc aaggtagtga tggaagacgg
    30601 agaagaagtg actattacaa atacatgtgc agccaatgtc cttggattag tgcaagaaga
    30661 acatttttct tatggaaatg tcccatctgc acgaaaagcc atccaagcat tagctgatta
    30721 ttgtcatacg acggttgaag acatcgcaga acagatcatg gaaaaatcct atgctaagat
    30781 tgaaccggtg attttagaat tggcagacaa gtatcacttg gagaaagatc agatttctct
    30841 tgtaggtgta gggggcggcg ctgcctcatt gattacttac tttagtaata aaatgggtgt
    30901 gaaatattcg attcctgaaa acgccgaagt gatttcttct attggtgtag cacttgcaat
    30961 ggtccgagat gttgtagaac gtatcatccc ttcgccaagt aaagaagata tacgatcgtt
    31021 gaaaaatgaa gcgatgaata aagcgattga atcaggcgct acaccagaat cgatcgaagt
    31081 ccacgtagaa atcgatccac agacttcaaa agtaacggct atcgctactg gttctacgga
    31141 agtgaaagca accgatctaa ccaaggagat cacaacagaa gaagcactag agcttgctgc
    31201 agaggatatg cgattaaata aaaatgaagt ttgcttattg gaaaatacac ctttctttta
    31261 tgtatgcggc gaacagaatc gttcaaaaaa tgctggaagt cttcggatca tcgatcaaaa
    31321 aggatttatc aaggtccaac gtggacatgc ttcatgtatg aaaacaactg cagccaatta
    31381 tatgacagct gtcgaacaac tttgggaaga tatggcagtt tatcaaactg aattgattgc
    31441 ccgaccagaa ttttatctct gcttaggagc aagagtctct gattttacag cgacggatct
    31501 ggaacaacta cagcttttga tggatctgga agtatctact atggaaccgg aggaagaagt
    31561 gatcgtcgtt gccggaaata tcaaacaaac ataagttaga agagttacta aacgaattac
    31621 aacagatccc cgaagaaatt tggggattct accagtttcg gcgagattta ttttggaaaa
    31681 agattccttt gtccaagcag aaaattctta tacaacaatc aatcgattgt ggtatcgaaa
    31741 cggcatgtag catcaaaaaa aagtaccctt ttgccgatgt aggagaaatt tgtgaacaaa
    31801 tggctgttcc aatcgtttcc tgtgaatcag agcaaataaa cgaacgaatc acttttgcaa
    31861 cttatgcaga agatgaaggg attcgattga tgacggagcc gttagaaaaa ttaaaatgtt
    31921 caggactagc ttcgatctct aaagaaacag cgcaagcttt gattattgga catgaactat
    31981 ttcaccacat cgaagcttct gttaagggaa tttatacgca aagcgaaaaa attgtgctgt
    32041 ggcggttgcc tttttataca catcaatcaa acattcgtgc attgagcgag atagcagcga
    32101 tgagtttttc aaaagagatg aatcaaagcg atttttcgcc ttatgtatta gaggcagtct
    32161 tactttggcc atataatgaa attcgtagcc aagggatctt agaagaaata aaaaaaatag
    32221 aaaaaagatg tacggagtat gatttagatt acaaataaag agactgtgac aaaagtcgtg
    32281 tcacagtcct ttttttccga ataaacggtg ttctcctgtc aactcctcgg tcacaagtca
    32341 aaatattcaa caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagatga
    32401 acggcaggct cacgacctct tggaaaacgg tgttcaaaag cttgcatctg cggtaataag
    32461 tccagccaag ttaaaaacat aagaagctat ttttacttgg ctgttcttat tacttgatgc
    32521 agggcagctt tttcacaacc tctcaataaa cggtgtgcaa tcagctgacc ctcggtatta
    32581 gctcaaaaaa aacggcaaaa tatgaggaac tattttgccg tttctttctc taatactcag
    32641 gtcaaaacgc tgattccaaa acctcttatt tgatgggtct gtcctgaata tcttcaaaat
    32701 aatcggggta attatgaatc acgtattctt gctcctcgat aatcatatgt aaatgcatcg
    32761 tgcaaagaaa tccgacttca tcaaagttat gatcaatcat tgattgaagc attgtctgat
    32821 gttcatctaa tactcgaccc cagtctaatt ctgaaatcgt caaacgtaac catctgaaac
    32881 ggttcaagtg cgtactataa ctttctagcc aatcccaaat ttctcgtttt ccagcaattt
    32941 cataacaagt tcggtgaaaa tcagtatcca aatcaaaaaa agctttttta ttttgagcga
    33001 ttactgcctg atcggtttgt tctagtattt tttctagttt agcttgatct tgcttggtca
    33061 ttttcgcact gcattcttgc aggatcggtt tttccaactt ttcccgcgta taacgggcta
    33121 atgaagcaga tcggatatcg atttttgaaa cataagtgcc ggactgtgga atggttttta
    33181 caagctcttg attgcgcagt tggatcagtg cttctcgaat cggtgtacgc ccgatagcta
    33241 gttgttcttc taacacttta tcggagattt tacttccagg tgctaactcg gaataaatga
    33301 tttgtttacg tatagattca taagcttgct gttgcaaatt attgataacc atttaacctt
    33361 cctttctatg cagatataat ctattataac ataaatcgct tacaaatcgt gtgggcataa
    33421 aaacacagtc tgcaaaaaaa gccctgatag gcatgcctat tagagcctcg aaaagaaaaa
    33481 tattgaatca atctggtcgt acctcttgga tcggtttgac acgaccgccg attcttttag
    33541 caaaagcttc ggattctttt ttgtctttga aaatgagcag attgtctaag tttttttccc
    33601 gagtgcgaca gccagtttta tcgatgtaac cgtgcattac tttaacgaca tacatgggga
    33661 tcacctgctt ttttcttttc attatacgaa ctactcgaca caatacaaga aaaatgctta
    33721 gacggtcttt attaagaatt tacatcatcc aaagaagcgt acgcttggaa taactgatct
    33781 aactccggat tgactttgct cagcttcact aatcgttgcg aatcagaaag taaaaaacag
    33841 tgtttactac tgccagtagt ttttaatttt ttgtcttcag ttcccacaat ttcgtagcta
    33901 aaatccaacc gtgtccccgt atacttttgg atagttggat ggatgctcac tttttctccg
    33961 taatggatca tttctttata gctgcaattt acttccagaa cggggatgat gatcccagat
    34021 tcttcgattt ttttataggg aaaccctaga tgtaccaata aggctacccg agcctcttca
    34081 aaccagcgga tataattgct gtgatgaata atccccattt gatctgtttc ataataaaaa
    34141 ggttctcgca cataacctgt aaatttctcc atgactcatg cctcgttttc cattgatttt
    34201 ttacaaaaat aattcatcca tttcggaagt gcttcgctga tttgtgtcgg caaaacaata
    34261 tattgttctt ttgccagctg gtctccaatg aaactatgaa gataaatagc tgctgcaatc
    34321 gtttggtcat tttgaggaaa ctgggctaaa aatcctgtga tcatcccagc taaagtgtca
    34381 cccatgccgc ccgttgccat agcagcagaa cctaaaggat tggtgtagaa gtgctggtga
    34441 ccataaattt ttgtctcatg acttttcaaa acgagcacag caccgatttc ctgttgcttc
    34501 tttttgtttt ctttatcccg ttgttcttgt atcggtagag aactcaaccg ttgccactcc
    34561 atttgatgag gtgtaagtac agtttgttcg ggatagggta gcaaaagatg attagcagca
    34621 agtaaactta aagcagagcc atcaatcacc agccattgtt cttttgattg gttttctagt
    34681 acttgtttga aacgttccaa actcttagaa gaagtaccca atccgggacc aatcaagagt
    34741 acatctgctt gctttattac ggaaagaaac tggtcagtat ccgtccaaac tacaatcatt
    34801 gcttctggca gatgactgtg aagagcactc cgattttttt catcagttac tacagtcgtg
    34861 agacccgctc cgctgtgtac ggcagctaaa gcactcataa tgattgctcc gccatattgt
    34921 tcattgccgc caatcaaaac gatacgtcca aatgttcctt tatgtgaaat ttttggacga
    34981 ggtgtgatga ctcttttcac tagttgttcg tttatcagca tctaaaaacc tccttttact
    35041 cattatagcg tatatttttt ttagagaaaa acagataaga aaaggaattg tcagattttt
    35101 tgaactattt ttttctgtaa tttgtggtat catgaacacg aagacttaaa taagtggagg
    35161 attaacttta tgacaattga ttggcaaaaa gaagtagaag cacgtaaaga agacttgctt
    35221 gaagacttga aaaatctttt acgtgtcaac agcgagcgcg atgactcaaa agtaacacca
    35281 gatgcaccat ttggacctgg acctcgcgac gcattgaaac atatgttagc ttatggcgaa
    35341 cgtgatggat ttaaagtaaa aaatgtcgat aactatgcag ggcatattga tcttggcgaa
    35401 ggagacgaaa cgctagggat atttggacac atggatgtcg ttcctgcagg agatggctgg
    35461 gacacagatc cttatgaacc agtaatcaag gatggaaaga tctttgcacg cggatcaagc
    35521 gatgacaaag gtccaagtat ggcagcttac tatgcgatga agatcattaa agaacttgac
    35581 ttgaaactat caaaaaaagt tcgcttcgta gtcggcagcg atgaagaaag cggctggggc
    35641 gatatggctt attatttcga acatgaagaa gaaccagatt ttggtttctc accagatgct
    35701 gaattcccaa tcatcaatgg tgaaaaagga aatgtttctt tagctttacg cttcaaaggc
    35761 gacaatgcag gagattatgt cttgaaatct tttgtttccg gacttcgcga aaatatggtg
    35821 ccaggaacag caactgcagc acttcaagta ccaagtgcag atgcagctat cgcgatggaa
    35881 gaagcatttt accaattcat cgaagcaaat ccagtaagcg gaacgattga agcggacaat
    35941 acgtatgtaa aaatcgaatt agttggtaaa ggcgcacatg gtgcaagccc acaatctggt
    36001 atcaatgcag gttctttctt ggcattgttc ttagataact atgaattcct aggatctgct
    36061 aaacaattta tccatgtagc agctgcttat gtccatgaag atttttatgg agaaaaacta
    36121 ggagtcgctt atgaagatga aaaaatgggc aaattgacaa tgaatgctgg actatttgct
    36181 tttgaagaaa atggtacaga ggaagcgaac ttcatcaata tgaatttccg ttttccaaaa
    36241 ggtgtaacag tagacggatt gcaaagtgac atcgaacaaa cagtggggca agaaggcgca
    36301 acagttactc gaggtgctcg cgtaatggaa ccacattacg taccaatgga tgatccattg
    36361 gtggctacat tattgcaagt ctatgaagac cacactggag aaaaaggcta cgaacaaatc
    36421 atcggtggcg gaacttacgg acgtttgttg aaacgtggcg ttgcttatgg agcgatgttc
    36481 ccaggatata ctgatacaat gcatcaagca aacgaattta tgagtcttga tgacttgttc
    36541 agagcaacag caatctatgc agatgccatc tatcgtttgg cgaaataatt aattaaaaag
    36601 aggatcaaga attgcaaatt atttttgcag tcttgatcct ttttttactt tctactgtgg
    36661 tattcatgat aaacatgata tagattcaat tcatattctt tcttagcgtt cgtgacgact
    36721 gtatccaaaa tcaaccctgt tactaatgaa agaagtgcta aagtcatcaa tccagtactt
    36781 aaaatcgcag aagggatgcg tgtaataaag cttgtcagca tatattcgcg aatcacagga
    36841 atccccgtga ccaatccaaa taaaaagaag aagatggtcc aaatcccaaa gaaaagcaaa
    36901 ggcttataat ctttaaacat tttaataatc atcatgatca ctttgaatcc gtcagagaat
    36961 gtattcaatt ttgattcgct gccttcagga cgatcacgat aatcgattgg gatttcaacg
    37021 aacttgaatt ttttatctaa agcatggatc gtcaattccg tttcgatttg aaaaccagaa
    37081 ctcataatag gaaaactttt gacaaagata cgattgaatc ctcgatagcc ggtcatgaca
    37141 tcaagaattt ttgttttata cattctagta attgtatttc taacaaggtt atttccaaaa
    37201 tcgtggaaag gtctcttatt ctcatcaaag taggttccat ttgataggcg atcaccaatg
    37261 accatatctg cttcaccaga acgtaatttc tcgagcaaac catgtacagc ttctgcagga
    37321 taagtatcat cgccatctac cattaaataa tagtcggcat cgatatcaaa gaacatttgg
    37381 cggatcacat ttccttttcc ttgacgaggt tcttttttta cgattgctcc accttgaacg
    37441 gccaattcgt atgttcgatc ggtcgaatta ttatcataga cataaatatc tgcttctggt
    37501 aattctcgtt taaagtctgc aatgactgtt gaaattgttg cttcctcatt gtaacaagga
    37561 atcagaacag caattttttc ttgttgttcc atttttcact ctccttaact atagaaaaca
    37621 aaagctaaat taaaaatacc tcataatcaa aggaaatgct agtttttccc gctaaatatc
    37681 atttatttaa aaagaaaata ctggaatata tgtttggaaa gctctatcta aaaaaccacc
    37741 cactacaaaa gaaggacatg atacctctct tttgaatagt gggtgcgtat tcgttctctc
    37801 acttttttga cgtattaatc tgttgcacct ttttttcttg aaatttctaa tacggttcct
    37861 gtatgagcat ctgctaaaaa ttcataataa acagtttcgc catcttctat tcgagaaaga
    37921 cctccccgat aagcactcga ccgaacggcg aatttcctaa taggttcctt ttcaaaagag
    37981 atccaagaac cttcgattgc accttctttt agaaaagatg acttgatatt ttcaagtact
    38041 tggtctgctg acaatacttt tttgcgatga taccaaatcg ttgaagctac cccgctcaat
    38101 aatcctagac cgattccaac tgctaacccg cctttaaaat agcttagttc gctttcttca
    38161 tacatgtgga catacctcct tcttctcact tgttactcct tgtgcttatt ttaccacact
    38221 ctcatatttt ctttgaaata aataaccgct accagaaaaa ataagctata ataaatacga
    38281 tcggtctgca gaaaaaatca tattttatcc gcagatcaat ataagtctga gaggagttta
    38341 gtctgaaatt tagacgaata gcatatggaa gaaaaaacat ttcaacgaat caaagagtta
    38401 acagaattac aaggaacaag cggttttgaa caagatgttc gcgcttatat ggaaagtcat
    38461 atggaaccgc tggtagatga gttacagtta gatggtttgg gcggcatttt tggtctgcgc
    38521 catcatgaag aagcggatgc accccgtgta atggtcgcag cacatatgga tgaagtcgga
    38581 ttcatgctga cacaaatcca agaaaacggg ctttttaaag tcgtacctct aggcggctgg
    38641 aatccttacg ttgtttctgc tcagcgtttt acacttaaaa cctctactgg taagaattat
    38701 ccatgtattt cttcttccgt tcccccacat ttattgcgag gaacaagcgg acaaaaatca
    38761 gtagaagtaa ccgatatttt gttcgatgct gggtttgaat caagagaaga ggcaatgagt
    38821 tacggtgtcc ttccaggaga cacaatcgtt ccttacgctg aaacgatcaa aacagccaac
    38881 ggaaagaata ttatcagtaa atcatgggac aaccgctatg gctgtacaat ggtattagaa
    38941 gctttagaag cgttgcaaaa tgaaacatta ggtcatacat tgattgcagg cgcgaatgtc
    39001 caggaagaag taggcttgcg tggttctaaa gcttctgtca ataaattcaa gccagatctg
    39061 ttctttgcag tagactgctc tgcagcagat gatacagtga cgaaaaatgg cacattcgga
    39121 catttaggag aaggtacatt gatgcgtatc caagatccag gtttgatcat gcttcctcgt
    39181 ttgcgcgagt acttgctgga tatcgctgaa acaaacaata tcccttatca atactttgtt
    39241 tcaaaaggcg gtacagatgc aggtgctgct catacacaaa acgaaggaat cccaagtaca
    39301 gtgattggcg tagtgggacg ttatatccat acgcatcaaa cgatgttcag tattcgcgat
    39361 tttgaagctg cacgagaaat gttgattcaa acactgaaag gtttggataa atcaacagta
    39421 aatacgatcg tatacggaaa ataatgcgat agtgaaacta taaaatctgg caaaagattg
    39481 ctttttgcca gatttttttt ataaaaagag gatcaatgtt tgttagttcg aaagaaaggt
    39541 gtataattta attgaaaaat acataaacag aaagtagagg acaaagaatg attatcccaa
    39601 atagtttaga agaattagca ggctacgtag aaactggaaa gagtgtattt ttctttactg
    39661 ccggctggtg cggagactgc cgttttatca aaccgcaaat gcctgaaatt gaaaatgact
    39721 tttctgattg gcaatttatt gaagtagacc gtgacaagta catagatgta gccgctgaat
    39781 ggaacatttt tggtatccca agttttgtcg tgatccaaga cggaaaagaa ttaggtcgtc
    39841 tagtgaataa agatcgtaaa acaaaacagg aaatcgaatc gttcctagaa agtgtaacaa
    39901 aaggctgaca tctgttagga ggggtcaaga tgaatcaaga atataagaat attttagtag
    39961 gtatcgacgg tagtgaacaa gcacttgctg ctttcaaaaa agcggtagaa gtggctcgcc
    40021 gtaataatgg gaaagtatat gtagcaaacg taatcgatca gcaattttat aactttatgg
    40081 gctatgcacc tattgatcaa aatctagtcg atcagcaaac tgaaacagcc aaggaaatga
    40141 tcgaagaatg taaagaatat gggaaatctg ttgattacac aaatatagaa ggaattattg
    40201 catatggttc tgttcgtgaa tccatggcag tcaaattgcc aaaaaaatat gagatcgatc
    40261 taatcatggt tgggcagtca ggtctaaatg cagtcgaacg ctttatgaca ggaagtgtag
    40321 ccagctacgt gatacgtcat tcaccttgtg atgtcttgat cgtttctgac gaagaagaac
    40381 agccaagtaa ttaagcaagt ggaacttgta cgaccttttt aatcaattaa ctaaaacagt
    40441 aaagggactg tgacaaaagt cttgtcacag tcccttgtta cgaataaatg gtgttccaga
    40501 agaggcttct tcggaaataa agcatgaaaa aaatttcatg cagtgaatgc aattttttgt
    40561 taagataaat ctcagcaaag gataatggag gaaagaacat gatttttgct tataacaaag
    40621 cacacgtcgg agacaccttg atggttatta tagccgacga taaaggaaca gaaaacactg
    40681 ttgaacgcaa atggaatgtt gctcggatca aagatgaaca aggtcaagtt gttgcttgga
    40741 actttttcca tatctccgat catctaacaa tcgaagggaa cggccaagta agcttaacag
    40801 atgaggaatt agcgaaattg aatcgattga tcaaagaagc aggattcgaa gaacagcttg
    40861 aagctgatca tgaaccgaag atcgttgtcg ggtacgtaaa aacatgcgtc ccacatcctg
    40921 attctgacca tttatcgatt actgagacag aagtagacaa tggtacagta ttgcaaatcg
    40981 tttgcggtgc acccaatatc gaagctggac aaaaagttgt cgttgcaaaa ccaggagcta
    41041 tgatgccaga tggaatgatg atctggccag gaactttgcg aggggtagat agctatggaa
    41101 tgatctgttc cgctcgtgaa ttgcatttgc caaatgctcc agcaaaaaaa ggaatcttag
    41161 tattgcctga ggatgccgta gtcggagaag catttccaaa ataaagaggt tgtgacaagt
    41221 ctttgtcaca acctcttttt tattaatttt cactattttg attttgtttt aatgccgatt
    41281 gatcgatcgt caattcaaca gtagttgttt tttctttcga gccttcatag taagtgattt
    41341 tgacagaatc tcccactttt ttcttataca aagcagattg aagttctaca ccagaagaaa
    41401 cttctttgtc atcgatctta gtaatgacgt cgtattgttt caatccagct ttttcagccg
    41461 gtgtagctgt ttggacattt gtcacgatga caccgttagt tacagatgaa ggaatcttca
    41521 agatttgttc ttgttgctga gtagaaacgg cagataagtc gaccattgtg atacctagag
    41581 ctggacgtgt cacttttccg tctttttcca gttgattgat gatattcact acatcgttac
    41641 ttggaatagc aaatcccatt ccttctacgc ttacattaga agtagattca gaagtacttg
    41701 cgatcttact tgagttgata ccgataactt gtccttcgat attaactaaa ggaccaccag
    41761 aatttccagg gttgattgct gcatctgttt ggatcgcatt gatattgact gtttcgttcg
    41821 attcattcgt acttgtcact tgtcggttca aggaagaaat gattcctgaa gtgacagaat
    41881 ttgcatattc agatcctagt ggggaaccaa tcgcaatcgc aggctcacca accttcagag
    41941 cacttgagtc accaaaagag gcaactgttt cgactttgtc tgaattgatc ttcaacaccg
    42001 ctaaatctga ataagcatct gttccaacta attcagcttt taccttcgta ccgtctttca
    42061 tcaagacttc aagaccttgc tggccatcta ctacgtgatt attcgtgact acataagcag
    42121 tattcccgtc ttttttgtag atcacgccgc ttccttcact ataagcttcc aaattactat
    42181 catctgagct gctactttct tcttgctgcc cgaatagtcc gccaaaaccg cttgattggt
    42241 tttgactttg taagttgata acagaaacga cagcgtcttg aactttatct accgctttgg
    42301 tgatatcaga atctacattg actttgacat tttccacaac tgtttcaccg gcagaattct
    42361 gattgccgct tgtagcagta gaactattgt tgccagtgcc catcgctgca taaaaaatgc
    42421 cagctgtcag tagtcctcca acgatccctc caacaagccc cagacctaac ttacgccaga
    42481 ggctattatt tttacttttt ttcatctttg gtgtcacatt ttttctatcc atgtgttcct
    42541 cctccaatta cattcattta tctactgtta gtatagtctt atttgttgac tttagtctac
    42601 tttgagactg tgaacttttt atgaaaaact ttaggaaaaa tcattgtttt cagatgaaaa
    42661 tatttgattt tctttttcca cagatactgt gtaaaactac gcggatagga ttggaaaaat
    42721 aagggataaa acatatccac agcctgtgta taaagtggat aaattcttat aaaacgtatg
    42781 tttgcctaat gaatgtgatg aaataaggta aaataacact gttttctgtg gataaacctg
    42841 tggatgttgt gcataactgt tggggaaatc tttttaacaa aaatagccaa aataaacata
    42901 tagacgaaaa aattgattat aataaacaaa gtgagcatga agaccaaagg agagaaaaca
    42961 tgaatatcaa aattatctca gtaggaaaat taaaagaaaa atacctgatc caaggtatca
    43021 atgagtatgt caaacggctg aatgcctatg caaaaatcga attgatcgaa gttcctgacg
    43081 aaaaagcccc ggagaattta agcgaagcgc aaatgagaca agtaaaagaa aaagaagggg
    43141 aacggatctt agcgaaaatc aaagaacaag aatacgtcta tgccttagca attgaaggga
    43201 aaaatcctac aagtgaatct tttgctaaac aaatcgacca attaggtatc caaggaaaaa
    43261 gccaccttgt ttttgttatc ggcggctctt taggactcag cgaagccgtc atgaaaagaa
    43321 gcaatgctca gatctctttt ggaaaaatga cgtatccaca tcaattgatg cgtctgatcc
    43381 ttgtcgagca aatctaccgt gccttccgga tcaatgcggg ggctccatac cacaaataat
    43441 gatatttttt aagagaaaaa attatcccta ttttaaataa tagcaatagg tagtggtagg
    43501 gagaaattta taaataactt ttttatttgc tttggtagcc aagtaagttg caaaagaaaa
    43561 atagatactg ttaaaaagat aatgtagaca tagaaacaaa tacgtaaaat tttagttaga
    43621 ataatggcaa ggagaaaaaa catgatagat gaaaaaaagt gtttaaaaaa tctattagag
    43681 gatatggatg atattgaaga aaactatcta aatggactgg gaaaacaaat atatttcaga
    43741 tacttaagct atctaaaaat gaaataagac atagtaattt tttagctttt ttgtttgctc
    43801 caaatgaaac ccacaattta tctgatgagt tttttaaaat gtttttgaaa aggtatatag
    43861 attctaatga tgataccaaa gctgctatca attattttga tgctttactt aatagctatg
    43921 aagatttaat tgtttac
//
LOCUS       DAZKCY010000010        42552 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000010 DAZKCY010000000
VERSION     DAZKCY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.010"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(117..1166)
                     /gene="recA"
                     /locus_tag="ACG8XC_000502"
     CDS             complement(117..1166)
                     /gene="recA"
                     /locus_tag="ACG8XC_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP9190226.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1275..2516)
                     /locus_tag="ACG8XC_000503"
     CDS             complement(1275..2516)
                     /locus_tag="ACG8XC_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFP9190227.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2756..3334)
                     /gene="pgsA"
                     /locus_tag="ACG8XC_000504"
     CDS             complement(2756..3334)
                     /gene="pgsA"
                     /locus_tag="ACG8XC_000504"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP9190228.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3543..4466)
                     /locus_tag="ACG8XC_000505"
     CDS             complement(3543..4466)
                     /locus_tag="ACG8XC_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFP9190229.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5205..6491)
                     /locus_tag="ACG8XC_000506"
     CDS             complement(5205..6491)
                     /locus_tag="ACG8XC_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9190230.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6484..7752)
                     /locus_tag="ACG8XC_000507"
     CDS             complement(6484..7752)
                     /locus_tag="ACG8XC_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9190231.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            8004..8465
                     /gene="mscL"
                     /locus_tag="ACG8XC_000508"
     CDS             8004..8465
                     /gene="mscL"
                     /locus_tag="ACG8XC_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP9190232.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8524..8970)
                     /locus_tag="ACG8XC_000509"
     CDS             complement(8524..8970)
                     /locus_tag="ACG8XC_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9190233.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9063..9308)
                     /locus_tag="ACG8XC_000510"
     CDS             complement(9063..9308)
                     /locus_tag="ACG8XC_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFP9190234.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9423..10001)
                     /locus_tag="ACG8XC_000511"
     CDS             complement(9423..10001)
                     /locus_tag="ACG8XC_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9190235.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10078..10608)
                     /locus_tag="ACG8XC_000512"
     CDS             complement(10078..10608)
                     /locus_tag="ACG8XC_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFP9190236.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10612..11472)
                     /gene="rlmB"
                     /locus_tag="ACG8XC_000513"
     CDS             complement(10612..11472)
                     /gene="rlmB"
                     /locus_tag="ACG8XC_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP9190237.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11465..11860)
                     /locus_tag="ACG8XC_000514"
     CDS             complement(11465..11860)
                     /locus_tag="ACG8XC_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFP9190238.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11857..13269)
                     /gene="cysS"
                     /locus_tag="ACG8XC_000515"
     CDS             complement(11857..13269)
                     /gene="cysS"
                     /locus_tag="ACG8XC_000515"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP9190239.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13266..13802)
                     /gene="epsC"
                     /locus_tag="ACG8XC_000516"
     CDS             complement(13266..13802)
                     /gene="epsC"
                     /locus_tag="ACG8XC_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFP9190240.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13888..14127)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14124..15584)
                     /gene="gltX"
                     /locus_tag="ACG8XC_000517"
     CDS             complement(14124..15584)
                     /gene="gltX"
                     /locus_tag="ACG8XC_000517"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP9190241.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15754..16911)
                     /locus_tag="ACG8XC_000518"
     CDS             complement(15754..16911)
                     /locus_tag="ACG8XC_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFP9190242.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16945..18315)
                     /gene="radA"
                     /locus_tag="ACG8XC_000519"
     CDS             complement(16945..18315)
                     /gene="radA"
                     /locus_tag="ACG8XC_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP9190243.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18378..18905)
                     /locus_tag="ACG8XC_000520"
     CDS             complement(18378..18905)
                     /locus_tag="ACG8XC_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP9190244.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            19135..19941
                     /gene="proB"
                     /locus_tag="ACG8XC_000521"
     CDS             19135..19941
                     /gene="proB"
                     /locus_tag="ACG8XC_000521"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP9190245.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            19938..21185
                     /locus_tag="ACG8XC_000522"
     CDS             19938..21185
                     /locus_tag="ACG8XC_000522"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP9190246.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            21261..21605
                     /locus_tag="ACG8XC_000523"
     CDS             21261..21605
                     /locus_tag="ACG8XC_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190247.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(21722..22048)
                     /locus_tag="ACG8XC_000524"
     CDS             complement(21722..22048)
                     /locus_tag="ACG8XC_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190248.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(22663..23865)
                     /locus_tag="ACG8XC_000525"
     CDS             complement(22663..23865)
                     /locus_tag="ACG8XC_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9190249.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            23982..24497
                     /locus_tag="ACG8XC_000526"
     CDS             23982..24497
                     /locus_tag="ACG8XC_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9190250.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(24696..25142)
                     /locus_tag="ACG8XC_000527"
     CDS             complement(24696..25142)
                     /locus_tag="ACG8XC_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP9190251.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(25189..26667)
                     /gene="msr(C)"
                     /locus_tag="ACG8XC_000528"
     CDS             complement(25189..26667)
                     /gene="msr(C)"
                     /locus_tag="ACG8XC_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP9190252.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(27021..27650)
                     /locus_tag="ACG8XC_000529"
     CDS             complement(27021..27650)
                     /locus_tag="ACG8XC_000529"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP9190253.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(27634..28098)
                     /locus_tag="ACG8XC_000530"
     CDS             complement(27634..28098)
                     /locus_tag="ACG8XC_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9190254.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(28585..29853)
                     /locus_tag="ACG8XC_000531"
     CDS             complement(28585..29853)
                     /locus_tag="ACG8XC_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP9190255.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(30084..30821)
                     /locus_tag="ACG8XC_000532"
     CDS             complement(30084..30821)
                     /locus_tag="ACG8XC_000532"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9190256.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(30888..31457)
                     /locus_tag="ACG8XC_000533"
     CDS             complement(30888..31457)
                     /locus_tag="ACG8XC_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFP9190257.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            31585..32457
                     /locus_tag="ACG8XC_000534"
     CDS             31585..32457
                     /locus_tag="ACG8XC_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9190258.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            32799..33671
                     /locus_tag="ACG8XC_000535"
     CDS             32799..33671
                     /locus_tag="ACG8XC_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9190259.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            33668..35626
                     /gene="nagE"
                     /locus_tag="ACG8XC_000536"
     CDS             33668..35626
                     /gene="nagE"
                     /locus_tag="ACG8XC_000536"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFP9190260.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(35958..36809)
                     /locus_tag="ACG8XC_000537"
     CDS             complement(35958..36809)
                     /locus_tag="ACG8XC_000537"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9190261.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            36932..37399
                     /locus_tag="ACG8XC_000538"
     CDS             36932..37399
                     /locus_tag="ACG8XC_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFP9190262.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(37662..38144)
                     /locus_tag="ACG8XC_000539"
     CDS             complement(37662..38144)
                     /locus_tag="ACG8XC_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9190263.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(38244..39107)
                     /locus_tag="ACG8XC_000540"
     CDS             complement(38244..39107)
                     /locus_tag="ACG8XC_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9190264.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            39705..40580
                     /locus_tag="ACG8XC_000541"
     CDS             39705..40580
                     /locus_tag="ACG8XC_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger domain-containing protein"
                     /protein_id="HFP9190265.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREREFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(40725..40877)
                     /locus_tag="ACG8XC_000542"
                     /pseudo
     CDS             complement(40725..40877)
                     /locus_tag="ACG8XC_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(41145..42476)
                     /locus_tag="ACG8XC_000543"
     CDS             complement(41145..42476)
                     /locus_tag="ACG8XC_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9190266.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT        12272 a         8959 c         7586 g        13735 t
ORIGIN      
        1 ttttatgtac ccactcgtat aattaaataa ctcacgattt ctttattcaa taaaaaaacg
       61 gcaggtcaag gagagtacct actcttcaga ccagccgttt ttgtttatca taatttttat
      121 tcttctacta aaggcaattc ttcttgtcct ttttcatctt ctgggacagc gacttcttca
      181 ccaataccat aagctgctct aactagagca gaaacttctg ccatcatttc aggatgatta
      241 gccatataga ttttagcgtt ttcccgtcct tgaccaatcc gatcttcttt ataagaatac
      301 caagcaccgc ttttatcaac aatgtctttt tctactgcca tgtctagaag ttctccttct
      361 tgtgagatcc ctaaaccata catgacatct acttcagcga ttttgaaagg aggtgctact
      421 ttgtttttta cgactttgat ctttgtacgg ttaccaacaa tatctgtacc ttgtttcaat
      481 tgttctgcac gacgtacttc caaacggatc gttgcgtaga atttcaatgc gcgtcctcca
      541 ggagtgattt ctggattccc aaacatcacg cctacttttt cacggatttg gttgatgaaa
      601 atagcgattg tttttgtttt gttgatcgaa ccagagagtt tacgcaatgc ttgagacatc
      661 aaacgtgctt gtaacccgac atgtgagtca cccatttcgc cgtcgatttc tgctcgtgga
      721 actaaagcag caactgaatc gactactacg atatctacag ccccacttga tactaaagca
      781 tcagcgatct ctagaccttg ttctcctgtg tcaggctgtg aaagaagtag ttcatcgata
      841 ttcacaccta atttttgtgc atattgcgga tctaacgcat gctcagcatc aatgaaagcg
      901 gccgttccgc catttttttg tacttctgca atagcgtgta gtgcaactgt tgttttacct
      961 gaactttcag gtccatatac ttcgatgata cgtccacggg ggtaaccgcc gacacctaaa
     1021 gcgacatcta acgcaagaga gccacttgga attgtagaga tttgttggtc gattttttcg
     1081 cccaatttca taatagagcc tttaccatag ctcttttcaa tttttttcag tgcagcatct
     1141 aaggctgctt tacgatcatc tgccaatacg ataatcctcc ttatatttac aaatcagagc
     1201 aacatttcat tatccttatc atatcttttt tagtgaaaaa aagcaagaaa aaaccgaaca
     1261 aatattcgca tttatcattt tcttaaaagc tgttttctca aaagatcaaa tccttgcata
     1321 accgcattat atctgatagc agcacgatct ctggcaaagt gatatttttc cgaaaccacg
     1381 ccgtctttag atgaaatagc gatccatact gttcctgcag attgaccttc taacgtgtcc
     1441 ggtccagcca ctccggtgaa agctacactg taatctgttc ttgctatctc acgtgcttga
     1501 atggccatct gctccacaca ttctttgctg actgttccat attctgctaa tagctccgga
     1561 tcaatctcca gaaaaccagc ttttgtttgg agcgagtagg taacgaatcc gcctggaaat
     1621 acttcagata caccagcaat gtcgcctaat gcggcttgga aggcgccggc tgtcaaactt
     1681 tccgctgctg ttacagtctg cttgttttct ttcagtagtt ccactacgac ttttgctagc
     1741 gaattatcat cgccgtatcc ataaaaatat tcccctactc gttcttggat tttttcttct
     1801 aatgctaaaa gagcttgatt ccctgcctga acatcatttg ttttcactgt cagtcgtaat
     1861 gtcacttcat ttggtttggc atatggtgcg atcgttggat tgatctgtgt ctcaatcaga
     1921 tcttttagct cagtgaccag tcgcgattca ccaatcccat aaaagcgcaa aactttagaa
     1981 attagttttt cttcagaagg aaaacgttcc tctagtaaag gacgtacttg ttccacaaac
     2041 attggtttca actcattagg cggaccggga agaaggatat acgcatgtgt atctgtttga
     2101 taaaaagtcc ctaaagcaag ccctgtacga tttggtagag gaaccccgcc ttcgataatc
     2161 tgggactgtt gcagattgtt tttggtcatt tttcgatggg ttgtttcaaa atatgccaat
     2221 aattttttgt atccttccgt attttgtatg agagacttac cgaggtgagc cgcagttgct
     2281 tcttttgtca ggtcatcttc tgtcggtcct aatccgccac ataagagtat gagttcactt
     2341 cttgtttcag ctagagcaat caactcttct aaacgctgct gattatctcc tacaactgtt
     2401 tgataataaa cttcgatacc tagatcagct agctgttctg acaagaaagt ggcattcgta
     2461 ttcacgactt gtcctagtaa aagttctgtt ccgacagcta ttatttctgc tttcattact
     2521 tcccgctccc ttatatagta tacgctgtat ttttatattt ttttatttaa aaagagggca
     2581 aggagctgtg acaaaaatct tgtcacagct ccttgttcca aataaacagt gttctcctgc
     2641 caaaacctcg gtcataagtc acaatataca taaaacatga aaagctgttt tttgaaatta
     2701 tcccttgtga ctcagagtta gacggcaggc tcacaacctc tccactaaac gatacttaca
     2761 ttgatccttt gaacacaccg ctgtttttga tgaagtaatc cacacctgaa tagatagtaa
     2821 agatcagaca agcataaagc atgatttgat ctactggaat accgatcagg ctgaatggga
     2881 tattattgat aaacagcaag ataatagcaa ccatttgtgt agccgtcttg acttttcccg
     2941 gccaagctgc agccatgact tctccatgtt cgacaagtag taaacgcaag cctgtgacgg
     3001 ctaattcacg acagacaatg atcgctacta cccaaccagg ggcctttcct tggtcaacca
     3061 acatgataaa agcagtcata actagcattt tatctgccaa cgggtccgca aacttgccaa
     3121 aattagtgac aaggcctcgc gcacgggcaa tttttccatc taaccagtct gtgatactag
     3181 ctaaagcaaa aataactgca cctactagct gtgtgacagc cagtgtggtg tctcctacgt
     3241 taaattgtcc ccaatctaaa ggtaaaccta caaccaaaat gaaaatcggg atcatacaaa
     3301 ttctaagtac agttaattta tttggtaaat tcacactatc gactcctttc ttccctattc
     3361 tacgtgacta tcagcgaagt ttccaccgat attatttact tttccaaatg aatttattca
     3421 aaggagtatt tttattgaaa ttgatctttt tgaattaacg aagaaggacc gcgacacaga
     3481 tttgtcacga tccttctttt ttatacctac aagtaaacag atgcgtaaac taatttatac
     3541 gatcattctg cgtattgaag agtaaggttc acattttttg gactctgatt gttttcccct
     3601 ggattaactg gtacggattc tccattcgct ttgatcgttg cattgcttgc tgcaccaatc
     3661 gtgatggtcg catttgttgc tgtttccggt aacacggtac tctgtgtttc atttgctgcc
     3721 agtgtccctt gataaacata ggcattgtct actagcacac ctaaccatac acgattgaca
     3781 gcagtaaatt caagggttac aggcttggta gcgtctgtga tatttactgc gatggcactg
     3841 ccagaatctt ctcctgtagc tattgccatt ttcttttctt cacttgattt tgttgttgat
     3901 tctactgtag aagactcttt ggtctgcgtg ctttgctctg aggaagagga ctcctcgact
     3961 gatccttcta ctttaagcga agaagctgtg tctccgatta ttggatcagc ttgacgatct
     4021 tgccaagtca tatagcccac gacaacgaca atcgccaaag caaccaatcc taataaaatg
     4081 acaggaagac ttgtccaaaa gcgacttgga tgcgattctt cctgatgcat tgcttttcta
     4141 gaagtactta ctgtttcagg ctgcggacgt tcagggaccg atcctgtcaa tttgcttttc
     4201 ccatcaaaaa catcgactaa atggtctcca tcttctccta cagcttcagc gtattgccga
     4261 ataaatgccc gcacgtaaaa tttaccaggc aattggtcaa aatcatcatt gtcgattgct
     4321 tccaaataac gtttttgaat ttttgtaatc tgctgtaatt catctaaaga tattttttta
     4381 ttcaggcgtg cttgccgtaa tttttctccg atggtttcaa aagccactcg ttcatcttct
     4441 ccagtctgtt tgattctccg aagcatctgc tgttgcaacg aaagacgtta tgtttgggtg
     4501 ctgtgacaaa agtcatgtca cagctccttt tttccgaata aacattgttc tcctgccaac
     4561 tcctcggtca caagtcacaa tatccaataa atataagaag ctgttttttg aacttgttcc
     4621 ttgtgactcg gagttaaacg gcaggctcac gacctctttc ttaaacggtg ttcaatcagc
     4681 tgttcctcgg tattagctca aaaaccggca aaatataagg agatattttt ccgtatgttt
     4741 ctctaatact caggtcaaaa cgctgatttc acaacctctt ccttaaacgg tgttcaaaag
     4801 cttgcatctg cggtaataag tccaaccaag ttaaaaacat taagaagctg tttttacttg
     4861 gctgttctta ttacttgatg cagggcagct ttttcacaac ctcttggtac acggtgttcc
     4921 agaagtagct tcttcggcaa taagccgaaa tatcacaaaa atttgagaag caattttcgt
     4981 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctctttg attacttcgt
     5041 ttgattttat tttaatataa gttgattaaa agagagcatg atttgtttta ttttctcact
     5101 aatttttcca acacaactaa gaataccata aaagctgatt ttggaggagg ttcctactac
     5161 aagtctaaaa cgaaatttaa gacttttcct cataaaatac tggtttattt tggtctcatg
     5221 taaaactcgc tgaaggcctc ttcacttata aatgcttccc cagctgccag tacgtctttc
     5281 atttggatcg aatcgatgat ttcaggaaga tcaaataaag ttcgatcgcc aaataaactt
     5341 tgcgtaaatt gattggcaat atattcgatc gaatttaatg attggaaata ttgtccaagc
     5401 attttcttct tcagcaaatc gagatttgtt tctgacactt ccggatcatc agcgaatcca
     5461 agaatgattt ctttgacttt ttctgctgct ttctctggtt cgtccgtatc tccgctgaag
     5521 tccgcaaagt gaaattcacg atccaagctg aattcaaaac caaagctgtc atcgataatt
     5581 ccttgattgt acatcgctag atagtttcga gaagtattgc ctaaaatcat ttggaacaac
     5641 agattgattg ctgttttata gcgtaacagt tctgttcctt cttgcggcaa tgtgtctaat
     5701 cctttaatac caagtacaaa tttatctcgt gtaatggctg cttccaatgc actttgtttg
     5761 atgatttctt ttgtgttttc agggaaataa cgtacaattt cttgctttgg agggaaattt
     5821 tttgcttctt ggttttcccg aataagtttc atcaatttct caggctccat cttaccaact
     5881 acaaataaaa ccatattgct tggctgataa aacgtccgat agcacgtata caagtcttga
     5941 gctgtgattt tgtcgatact ttcaaccgtg cccgcaatat cgatatgcaa tgggtgtgta
     6001 ggatacaagt tatttagtat gccaaaaaac atccgccagt tagggtcgtc ttcatacatt
     6061 tggatttcct gaccgatgat tcctttttcc ttgtttacag tttcttctgt gaagtaaggt
     6121 gcttgtacaa aatcgatcag agtcgtcaga ttcttttcta cctgatccgt cgtagaaaac
     6181 agatagctgg ttttcgtaaa acttgtgaaa gcattggcgg aagctccttg tttcccaaat
     6241 aattgaaaga catcaccatc ttctttttca aacaacttat gttctaaaaa atgggcaatt
     6301 ccatctggaa ctttcacctt ttctttttca ccatagggaa taaattcgtt atcgatcgaa
     6361 ccataattcg tactgaacaa accatatgtc ttgtgatagt catttttagg caacaaatac
     6421 acggtcaaac cgtttggcag tacttcatga taaagtgttt cattgatttg ctcatattct
     6481 gttttattca tttgcatttc ctcctgctaa aaagaagatt gcctgcaatt cgatttgctc
     6541 agctacttgt tgtatctctt tgatcgttac gctctccata cgtttcaacc attcttcatc
     6601 agataatttt gtttccggca gccagctgtc caaaaaagaa gcttcgattg ccgcttgcgg
     6661 agagtctaga gaaagcagat attggtttct cagcattgct tttgtctgag acagttccaa
     6721 ctctgttatt tcaccatttc gcaaactttc taattgctcg tgaatcagat gaagtacttg
     6781 atttcggttc ttttcatcga taccggtttg gacactcatg aatcctcgga acgtatccac
     6841 gctactagag gcataataag ctagactttc tttctctctg acattcatga aaagtttaga
     6901 gtgaggaaaa ccgccaaata aaccgttaaa gaccattagc gcaaaacgtt ctggttcatc
     6961 gtaatacaca tttgtctgat aagctaggtt cagctttgct tgaacgattg gctcacgtac
     7021 ttgtcgttcc ttcacgatat tcacttgagg ttgggtataa aacagatctg gataaactgt
     7081 ttcccgcgac tcaaatggta attgcccaac tgcttcagct gctttgtccg gatcaacatc
     7141 gccgacaaca aaaatgtcga tttgatcatt tgccatcatt tcttgatagt aggacgctag
     7201 cgaactagca gtgatcttgt ccaaatcttc gactacgccc aaccctggga tcttttgttc
     7261 cgggctgttt tcaaaataaa gttcttgcaa ggcataggaa gcaaaagttt gtttatcttc
     7321 cttcaaactt tccaaatata gacgcagatt gttcttttcc aagtcaaacg tcaactgatc
     7381 aaactgatga tttttaatat ttggataaaa taaaacctct tttaaaaaat ctaccgcttg
     7441 agaaaaaagg tttggatcat tgacatattc accattcaca aaactgatcc ctgcgtttag
     7501 ccaatgtaaa tttccttttt ttctaactga taatccaaag cttgcgccat aaagctctgc
     7561 aagttgcgca cttaactttg tctgatccga ataattcaaa ctatttgttt ccaacatgct
     7621 gctgagaagt gaacgtttcg tgatgacttc ttgttgcaga cgcgcagtaa aacggataaa
     7681 aatccggatc gttttgtatt gttttgttgg taagacatga agattcacgc cttctgccaa
     7741 ttgatacacc atatttatat gctccttttc acagttgatt gcccgcttac tcttttaatg
     7801 ggtcaacctg ccttttttac ttattcctct gcttgaaatt tataagttca ggcaattcgc
     7861 cgttttttct aaatgatgtt acactttata gtatagcata gatgtttttt atcaaaagaa
     7921 acagacggct aaaagcgtat attgtttttt tattttttat ttttcgatat actaaagaca
     7981 aataaaagaa gggagggtca ttcatgatca aagaattcaa agagttcatc atgcgtggag
     8041 atgtcttgga tcttgctgtc ggcgttgtta ttggtagtgc gtttacaagc atcgtaaacc
     8101 aagtggtgca aggcttgatc acaccactaa taggttggtt tgtcgcaatg atcacaggta
     8161 cttctgattt agaaggagca ctttcaattc ttgactggtc gccgacaaaa ggcgtcacat
     8221 ttgcgtttgg taacgttatt agtgctatca ttacatttct gatcactggt tttgtcttat
     8281 tcttagttgt caaagcggca aaccgtgcga agaatttacg ctccaaagaa gagcaagtgg
     8341 aagtggaaga aactcctact gcagaagatt atcttagtga catacgcgat cttcttgcca
     8401 aacaagtggc agaagacaat acacatccag tagagcaaac aaatctttcc gatacttcta
     8461 aataataaag aatctaaaaa aaagaaagcc gattttcagt gtcggctttc tttttttaga
     8521 ttcttaactt gctacttgtt cctttttcat gtcgatatgt ggaatatcat cttccaaata
     8581 cacatctgag attgccttga atccaaacga ctcataaaaa gcttgtaggt attcttgtgc
     8641 gccgatcacg attggttttt ctggaaaatt tttctggatc atgttaatcg tttcttgaac
     8701 aatctttctt cctaaaccat ctttacggtt gtcctgatga acaaggacac gtccaaaatg
     8761 gatactctcc gcttcttcat atactcgcgt gtaggcaagg atcttcccat cagtatcttg
     8821 aaaaaacgta tgccaagctt ctccatcaat gtcatcaact tcttgataag gacactgttg
     8881 ttccacaaca aaaactgcga ttcgttcttt gacgatcgcg taaaactctg ctgttgacaa
     8941 ttcttcaaat ttctttacct gatattccac taagcaaacg ccccctgctt ttttattctt
     9001 agcataccga ttcgcacaca aaaaagcaaa cgaaccttac gaatctcagg ctcgtttgct
     9061 ttttaaactg cttcagttaa aaattcgatc tcaactgtcc gagtaagtac atctgaatag
     9121 ctgtaagata ctcgttcaaa agaattttct tcaggatcca aatcaacaac gaatactgaa
     9181 ggataggttt ctgttaaaac gccgcggcgt tctgtttgac gctttcttcc tgtctgagca
     9241 accaacatga ttgggctgcc gatacgacct tctaaatctt tcttgattga agctaaagtt
     9301 gttggcatac agttcacctc tactatcaac tataccacat atttttaaaa ttttcaagtt
     9361 taccataaaa aaaaaagaaa tgcaataaaa ataaataaga agctcattaa gatttttcta
     9421 aattattcct cattataaaa tttcaagtgt ttttttattt tgagttttac tctcccgtaa
     9481 gcgctcatta cttgcttgtg atccattgct aaacattcag ccatgtctac aaaatcaacg
     9541 ccttgcatat aataatgaaa aattttagct tctatttttg aaaactggat ttcgcaattt
     9601 tcgattgctt cttttaataa aagatgctga attgctaaag gttcttgcaa tgtgatctct
     9661 gctaaaaagt ctggtccttc tgtctctatt ttttggtcga ttgagacttc atctttttga
     9721 tcacgacgct tcagcgcgtt ctgcttcctc aacagagaat agatttgatt gcttataacg
     9781 cttttataat aacttccaaa gctaacacct ttgctttttt cgtaagttct caatgcctga
     9841 tataaagcga tccttgcttc ttgttcccaa tcctcagaaa cataatttct taaataatag
     9901 gtgtttttca aacgtagtaa aagtggaaaa tatttttgat acagggcagc aaatgcttct
     9961 tcgtcaccat tacgtgcagc gcgcaataac tcctcagaca taaaataatt cctccataaa
    10021 ttcgtttatg gaggaatctt atcatcaatg attcatcgaa ctgattagaa cgatatgtta
    10081 tttatttttt ctcattaaat cgtccaattt ttgggacaat ttttttaact gctcttcatt
    10141 ccatggagaa ttacgtcgga aatcaataaa atgcatatct ttattttctt gggctattgt
    10201 tttttccgtt ttttttatcg tcttatataa ttcataagca gaagttcgta atgctccttg
    10261 ggaaaagatc atccactgtt ctgctaaatc gcttgtcgcc actgtcactt gtgtcaaacg
    10321 gtcattcagc tgaccagcga ttctctcgat ataactgtca gccgtttcgt cttctttcgt
    10381 aaaaacaaca tccaactggt atttcttata attttgttgg atgcccggaa ctagttgggc
    10441 atcgaagaca acgatgactt ccattccttc atatttcgca taatttgata aacgatgtaa
    10501 aagagcttct cttgcatccg ccattttgtc ctgcttttta agctggacaa gttccggcca
    10561 cgaaccaatc atgttgtagc cgtctacaat caacagctgt tttttcatgg tctactcccc
    10621 ctgacgttgg gaaaaagcct gatacataag caaaccagca gcaacactgg catttaagct
    10681 ctggacgtgc ccggtcatcg ggatcgtcag caattcatcc atttcttttt tcaatcctgg
    10741 actcattcct ttgccttcat taccaatgac taaagcaatt gatcctttgg cattccattt
    10801 ttggaagttt gtgccggtca tatctgtgcc aaatacccaa aatcctgttt ctttgagctg
    10861 ttgcacagct tgtaccaaat tcgtcacgcg tgcaacaggg acatgttcga ctgccccagt
    10921 ggaagtcttt gttacgatag gtgtgattcc aactgctcgg tgttttggta tgatcacacc
    10981 atcgacgcca cttgcgtctg ctgtccgtaa aatcgaccca aaattgtgtg gatcttcgat
    11041 gctatccaaa atcaaaaaga aggggtcttc ttttttctct tttgttcgtt caatcagttg
    11101 gtcaagggat aaatattcat aaggtgtaat cgcaagaacg actccttgat gcacgccgtg
    11161 atcgctcaaa gtttctaatt tttgtttagg aacccatttg actggcacag cttgttcacg
    11221 tgcgatttct ttcagttctt ctactttttc tcctcgtgaa tcttcttgaa gaaagagttt
    11281 attccctcgc ccttgttgta gcgcttctac tgcggcatgg tgcccaaaga cgaaattatc
    11341 ttctaatgct tcttcatttt gatcagaaac tgactgattt cgtccagttt gctttttatt
    11401 tgaatatcta ttttgttttt tgtcttcaaa tccacgtttc gattgatgtt tgtttgttct
    11461 ttctttaccc atttttttca ccaactttct gaatacacca gcggatcaat tcttccatcc
    11521 gctcggtttg ttttgtcaaa tgcagatagc ccatgagcgc ttcgaaccct gttgcgaccc
    11581 gatacgtcgt aacatcagca ttttttgcgc tcgtatggct tttagcattt cttcctctgc
    11641 gataataaag ttcttcttct tctgtcaaaa gatgttcttc cagcatttgc tgaatcaaaa
    11701 aggcttgtgc attcgcagaa acataattcg ttgccgcacg atgcagctta ttgggttttg
    11761 tttctccttg atcaatcagg tggtcacgaa catagatttc gtaaatcgca tctccaacat
    11821 aagccaaggc taaaccgttc aattgtgtgt aatctctcat gcttctcttc tccatcgggt
    11881 tccttgcggt gtatcttcta ataagatccc tttttctttc aacaaatcac gaatttcgtc
    11941 gcttcggaca aaatcacgat tgcttcttgc ttgatttcgt tcttcgatca attgttcaat
    12001 ctcttcatct aataactctt ctgctgtaaa taaaataccg aagatcgaaa gccattgcga
    12061 gaataaagct tctccttgtg ccaagacagc ggcagaaact ttcggctggt ctagataagt
    12121 gttcagccac ttagcaagct catagaccac tgtgatccca tttgctgcat taaaatcatc
    12181 gtccatttct tcgatgaatc gctgttctaa attttctaac aaagcgattt tatccgcatc
    12241 atctgccaaa ttctctgagg cagtttcttg gcggaatttc aaattgtcaa acgtattctt
    12301 cagtcgttgt aaattggtgg ctgcttcttt catcgtcgtt tcactgtagc ggatcggacg
    12361 acggtattgt gtggtcgcca tgaagaaacg caagacttgt ggatcaacgt ttttgatcat
    12421 ttcatgtaca gtgatgaaat tccctagaga tttactcatt ttttcatcgt cttcgccaat
    12481 cgttacatag ccattgtgca tccagtaatt agcgaatttt ttccctgttt tcgcttcact
    12541 ttgtgcgatt tcattttcat gatgaggaaa ttctaaatct tgcccgccac catggatatc
    12601 gatcgtttct cctaaatgtt ttgttgccat aacggaacat tcaatatgcc aaccgggacg
    12661 tcctttgccc catggtgaat cccaagaaat ctcgccaggt tttgcttgtt tccataaagc
    12721 gaaatctaat gggtcttctt tttgctgctg ttcttcccct gtacgctgac ttgctccgac
    12781 ttccagttca tcgatggatt ggtcacttaa tttcccataa ttttcaaatt tacgggtacg
    12841 gtaatacaca tcacctgaag attcataagc atagcctttt tcaaccaaca cttgaataaa
    12901 tgctaaaata tctggcatat gatccatgac tcttggatgc agtgtagcag gctcgacatt
    12961 caacgctttc gtatcttctt caaatgctcg gatgaaacga tcagcaactt ctggtgctgt
    13021 aatacctaat tcgtttgctg tacggatgat tttatcgtct acatccgtga aattcgatac
    13081 gaaatcgact ttatagcctc gatattcaaa atagcgtcga attgtatcga aagcaatggt
    13141 gctacgagca tttccgatat ggatataatt atacacagtc ggtccgcaga catacatacc
    13201 gacatgtccc ttttgtatag ggatgaattc ttcttttttt ctggtcaatg tattgtagat
    13261 ttttatcatt ctgatctcct cactttttct cctttgattc gaacgacttt tgctggtact
    13321 cctaccgctg tagcgtcgtc tggaacatct ttcaacacaa cggcacctgc accgattttg
    13381 gcgttttttc cgatcgttac cggtcctaat atctgcgcat tcgcagagag catggctcct
    13441 tgtttaacag ttgggtgtct tttacccgtt tcttttcctg tgccgcctaa tgttacgcca
    13501 tggaataaaa tcacatcgtc ctcgatttct gcggtttcgc caatgacgat tcccattcca
    13561 tggtcgatga acacgccagt tccaatcgtt gctcctggat ggatctcaac accagttagg
    13621 aaacgccaga attgagcatt gatcttcgcc aataaaaaga gccggtgctg atataaaaaa
    13681 tgagagaaac gatgccaaaa caaggcatga aaaccagggt atgtcaaaac gatctcagct
    13741 gtcgaacgtg cagccggatc gtttctcttt gctgcatcaa tcgctcgggt aaaccaactc
    13801 atgactttcc actcctttcg taagaatttt atgtaggaaa tcatcttctt ataaagaaaa
    13861 gaagcgcctt ttaaataagt ttccttatca aaagacgctt tccgcgtggt tccacttttc
    13921 ttcataatca atgattatct ctgacagcta acgcctgtta tacgttcttg gctactttgt
    13981 ttcctataaa acagtttttc ctgttttctg tgtttcgcca agaccactcc cagaggcatt
    14041 tcggcaacca ttcattgata ccttgcacca cccggtatct ctcttgtaat gatcagctgc
    14101 ttactttttt ctgttcatcg tgtttagttc agtacttgat ttaaatgtgc taatgatttt
    14161 tcacgaccaa gcaattcgat cgtttcaggc aattctggac catgcatttg tcctgataca
    14221 gctacacgga ttggcatgaa aagatttttt ccttttacgc cagtttcttt ttgaacagct
    14281 ttgatggcag ctttgacagt tggagcatct actacttcca tttgttctag ctgtgctttg
    14341 aatgcgtgga gaacagtagg aactgtttca ccagcaagta cttcttttgc tgcttcattc
    14401 aatacaggat gttcattgaa aaatagcgta gacagatcaa cgatttctgc agcatagctc
    14461 atttgaggtt gatacaggct aacgatcttc ttcacccatt cgattgtctt ttcatctggg
    14521 ttttctgcta cacgtccatc agcgattaga taaggcagac acatgtcagt aaatgcatct
    14581 agatccattg ctttgatgta atggttgttt acccattgca gttttttcgc atcgaaagct
    14641 gcgggtgatt tacttaggcg ttttggatcg aacattttga tcaattcttg gtgttcgaag
    14701 atttcgtctt cgcccacagg tgaccagccc aataatgcaa taaagttgaa cattgcatct
    14761 ggtagatagc ctaattcgcg atattgttcg ataaattgca agattgattc gtcacgtttg
    14821 cttagttttt tacctgtttc actgttgatg atcaatgtca tatgtccgaa ggttggtggt
    14881 gtccagccaa atgcttcata gatcatcagt tgttttggtg tattggcaat atggtcatct
    14941 ccacgtaaaa cgtgagtgat tttcatcaaa tgatcatcga ccgcaacagc aaaattataa
    15001 gttggcatac catcgcgttt ttggatgaca aagtcgccac cgatattatc ggattcgaac
    15061 gaaatagcgc ctttcaccat atctgtaaat gcgtattctg tattgcgagg cacacggaaa
    15121 cgtacaacag attctagacc ttgtgcttct ttttctgctt gttcttcagg tgtcaagttc
    15181 gcacatgttc ctgcgtaatg aggcatttct cctcttgcgc gttgtgcttc ccgttctgct
    15241 tctaattctt cttctgtgca atagcatttg tatgctcgat tgctagcaag cagctgatca
    15301 atcagcggtt gatagatttc tttacgttca gattgacggt aagggccgta ttctccagga
    15361 ttttcaggag attcatccca attcattccc aaccaagcaa ggttttccaa ctggcttttt
    15421 tcgccatctt cgatattacg tttttggtca gtatcctcaa tacgaataat aaagtctcca
    15481 tcattgtggc gggcaaacaa ataattgaat aatgcagttc ttgcatttcc aatatgcagg
    15541 tgcccagttg gacttggtgc atagcgtacg cgtactttcg tcattttctt cctcttcttt
    15601 caaactagac tttgactaac agcaaaaatt ccgtcagtta cggcttctca aatatcattt
    15661 atttaaagcc actcagcaca aattttacct tttctaaggt aaatagtaaa gccaactggt
    15721 tgaatttcct taccagttgg ctgaatttct tttttatttt gattgtttag tctttttctc
    15781 tgtctctttt ttattgtcga ttcctcgtcc tgcatgtgca ggtttagcaa agatcatgcg
    15841 tccagcagct gtttgaaggg cacttgttac aacgacttcg atacgttcat tcatgtaatg
    15901 ctgtccatct tctacgacaa ccatcgtgcc gtcatcaagg taggctactc cttgttggcg
    15961 ttctgtcccg gcttttacta ctagaacatt catcgtttca ccagggatga cgactggttt
    16021 tacggcattt gccaacgcat tgatattaag aacaggtaca ttttggaatt cagagacttt
    16081 attcaagtta tagtcattgg ttacaatgac accatctaaa agcttagcca atttgatcaa
    16141 tttgctgtct acttcactga tatcttcaaa gtctccatcg tacatttcga cagatatacc
    16201 ctcttctttt tgcaaggcat ttaggatatc taatccgcga cgaccacgca cacgtttcaa
    16261 actatcccct gagtccgcga tgtattgtag ttcgtaaaga acaaaattag ggatcatcaa
    16321 ggttccttct aagaatccag tctttgtaat atcataaatc cgaccgtcaa tgatcacgct
    16381 tgtatccaag attttatatt tatggaaatt ttctcctgat tttcgttcta acagttgact
    16441 ctcaacttga ctagctgttt cttgctcgat ttttttgctt cgtgaaccaa aaactttctt
    16501 ccattcttcg attcgtgtcg ttcccattct caatcccaga tagcctaaga tgatcatcag
    16561 taaaatcggc aatacactat tgacgaatgg aatttccata ttgtaaagag gggtcgagat
    16621 cacaacacct aaagtcaatc cgatgatcgc accgatcgca ccgaagagaa gataagtcaa
    16681 gctgacctca cttagttttt gttctacttg tttgataata gctgagatat gtttggccaa
    16741 cagcaaagat aataaaaaga aaataagtgc gcctaacagg ctgttggtaa atgtattatt
    16801 cagccatccg tttgactgtt gaccgattgc ctgccaagct gagggtaaaa atgaaatacc
    16861 caagcttgcg ccgacaatga tcatcagcaa agtaatgaca cgtttttgca tcgtttttcc
    16921 tccgtttcga caatcattta ttagttagcg gaacacacgc ttcaacgttt cagccaaagt
    16981 tgctactcca actatctcga ttccttttgg cgcttcccag ttacctaagt tgtttttagg
    17041 taggtatact tttgtgaagc cgagtttttg gacttctttg actcgttgtt caatatgact
    17101 gacacgtctg atttctccag tcagtccaat ttcaccgatg aaacattccg ttggctgtgt
    17161 tcctttttct ttatagcttg aagcaatact gacagcgata gctaaatcaa tcgctggttc
    17221 atttaatttt accccacctg cagcttttaa gtaagcatct tgattctgaa gaagcaaccc
    17281 agctcttttt tccagcactg ccatgatcag agatacccga ttgaaatcaa gacctgttgt
    17341 tgttcgtttg gcatttccga acatagtagg tgtgactaat gcttggatct ctaccaaaat
    17401 tggacgtgtc ccttccattg ccacaacgat agcagagcca gttgcaccgt ctaggcgttc
    17461 ttctaagaaa atctgtgaag gattagcgac ttcttccaat ccatgctcat gcatttcaaa
    17521 aatcccaatc tcattcgttg agccaaatcg gttttttact gctcgcaaaa tccgaaaact
    17581 atgatgttgt tcaccttcaa aatataacac cgtatccacc atatgttcta acattcttgg
    17641 gccggcgatc gagccttctt tggtaacatg accgacgata aaaatggcta tcccatttgt
    17701 tttagcggtt ttgagtagtt ctgcagtggt ttctcttact tggctgacac ttcctgccac
    17761 acttgtgatg tctggctgcg tcatcgtttg gatggaatca atgataacat aatcagggga
    17821 gatatgttcg atggcacgac tgatttcgtt catatctgtt tcagcgtata gataaaattc
    17881 tgtgttgata cttccgaggc gttccgcgcg taattttatc tgctctgcgc tttcttcgcc
    17941 ggatacatat aagacttttc cgcctatggc tgctaattgc tgagaaactt gtaacagcaa
    18001 agtggatttc ccgatacctg gatcgccacc aatcaagacc atagaccctg gcaccacacc
    18061 tccgcctaac acacggttca gttcttctaa ttttgttttg atccgaggtt cttttttagg
    18121 gacgacgtct gcgatttttg ttggttttgc tttttctcct gtcaaacttg tccgagttct
    18181 gcggtccgtt gtatcttgtt cgatttcttc gaccatcgta ttccattttc cgcaatttgg
    18241 gcagcgtccc aagaacttag gcgacacata cccgcaattt tggcaaacaa attgtacttt
    18301 tgattttttt gccattgtac cgcctccttt tatcctaatc atgagacatt aaatcaagct
    18361 taagtatctt taacgtttta tttactggaa gaaccaaatc cgccttgacg ttgagcagtc
    18421 gtttgatctt gatctgcttt taaaaacggc aaaaagatcc cttggccgat acgctcgcct
    18481 tttttgatca ctacatcctt gaaaccaaag ttggtgaatt ggaacatgat atggccttca
    18541 tttgcctcat tattgtaata atcactgtca atcacaccta ctccgtttgt caacactaaa
    18601 aaccgcttca acggattgct tgaacggtta gcaagctgca aaaattcatc ttctcccatg
    18661 taagctttga ttccagtagg cacgagggtt ggtttcaatt gtttttcatt ttctgataca
    18721 tcttttttca atccatctgc gactgtttcc aacccctttt tccagcctgc tttccagata
    18781 cttggaatca cgacctcttc tgctgcttca aaatcatagc cagcagcatg atgtgtagcg
    18841 cgctgcggta gattgatatt ttgatcttta tatttagaaa taacttcaaa tcctcttttt
    18901 ctcaccggtt ttcctcccta aataaactac tcctattcta acatatccga ggctgtaaaa
    18961 gactttcttt acaaattctt caagaaattc tgctatctta aagtaaacca ttaattcgtt
    19021 ctttttccaa acgactgcaa gttgtacaaa aaaactattt tttacatgac tttactcgtc
    19081 aaaacaagta gaatagaacg aaattaatga gtggatattt gattgaggtg tcacatgcgt
    19141 aaagaaatta aaaatgcaaa acgaattatt gtcaaagtag gaacaagtac attgatttat
    19201 cccaatggaa atatcaatct tgcggcaatc gatcagttag cttttgtcct ttctgatctt
    19261 agaaatcggg gaaaggaagt gatcttagtc tcatcaggag ctgtaggcgt tggtatgcat
    19321 aaattacatt tgtcagaacg tccgactttg attcctgaac agcaagctgc ggcggctgtt
    19381 ggtcaagccg aactgatgaa tatctataat caaagattca gtgtatataa tcaacagacg
    19441 gcacagatcc tcgtgacaag agacgtggta gattatcctg aaagtcgtaa aaatgtgagg
    19501 aatacattgg aacagctttt gattatggac acgatcccta tcgtgaacga aaacgatact
    19561 gtagcagtgg atgaattaga tcatctgaca aaatttggcg ataatgatca gctgtcagcc
    19621 atcatggcaa aacttgtcgc ggcagacctt ttgatcgtcc tttctgatat cgacgggttt
    19681 tattcagata atccgactac aaatcccgag gctcagatgt atcgctatat cgataaaatc
    19741 gatgaaaaga ttctggcaca agcaggtggt gctggtagca catttggaac aggcggtatg
    19801 cagagtaaat taaaagccgc ttctaggata tttgaagcaa acagcgcaat gattttagca
    19861 aatggaaaaa atccggcaat tatttttgat attcttgcag gaaaagaagt aggtacttta
    19921 tttaaggagg acttgaaatg actgacttga tgcaattagg acaacaggca aaagatagcg
    19981 cgcaacaact agcacttatg cctacaaacc gcaaaaatga gctacttgtt caaatggcac
    20041 aaaccatcaa aaagaatcaa caagcgatca ttgaagcaaa tcataaagac ttagaaaaag
    20101 cagtcgaaaa caatatcagt gaaacgatgc aagaccgctt gcagctgaca cctgagcgaa
    20161 tcaatgccat ggctacagag atcgaaaaaa ttgcttcgtt agacgatcct attggaaaag
    20221 tagatgagat gtggacgaac accgacggat tgagaatcgg caaaaagcgc gtgccactag
    20281 gcgtgatcgg catcatttac gaatctcgtc caaatgtcac gacagatgca gctagtctag
    20341 cattcaaatc agggaatgcc gttatccttc gcggtggcaa agaagcattc ttctcgaatc
    20401 aattattagt ccaattattg cagcaagtac tggtcagtgc tggcgaatct ccttatgcca
    20461 tccagtttgt cgatgatact tctcatgaaa ctgcaggaca gttgatgcga ttgacagaat
    20521 acttagatgt gctgattcct cgaggcggag caaaattaat ccaacgagtc aaagaacagg
    20581 caacggtacc tatcattgaa acaggtacag gaaataatca cgtatatatc gataaagaag
    20641 cacagttatc tatggcagtg aacatcatcg tcaatgcaaa agcttcccgt ccatctgtct
    20701 gtaacgcggc agaaacattg ctgatccatt ctgagatcgc accttttttc ttgccagcta
    20761 ttgaaaaaga actggtagaa cacggggtct ctttacgtgc ggatgctcgt gcgttagaat
    20821 atctagagac ggctgcttta gcagaagaca cagattggga tactgaatat ctagattata
    20881 tccttgctgt caaagtggtc gattctttgg atgaagcaat tgcacatatc aatcggcata
    20941 atactaaaca ttcagaaacg atcgtgacag atagttatgc ggctagtcaa cgtttcttaa
    21001 atgaagtgga cgcggcggct gtctatgtca atgcctccac tcgttttaca gacggatcgg
    21061 tatttggttt tggtgcagag attggtatct ctactcaaaa gttgcatgcc cgtggtccaa
    21121 tggggttaaa tgaattaact tccacaaaat atatcattta tggcgatgga caaatccgcg
    21181 aataatgttt cgtgtgaaac attattcccg ccttttgcct tttttctgta aaggttctat
    21241 ccaactagaa aggtgtcaat atgtttgaaa ttttaatgat tttttttatt taccttattt
    21301 ctctgaatat cgctgctttt ctgggagtcg gtattttatc ccttttcttt caattcaaaa
    21361 aacgaagcat agggagtcaa cgggaaaaat ggtcgcagta ttttgacaaa atcgggcaaa
    21421 aagggctggt tacacgcttg cacattagtt acatggttgc attatgctta ttagcgacaa
    21481 tcaattacta ttcttttttc gatcattcaa tcgcttatac gattacgcta ctgattgctg
    21541 ggatttttca cttatcttat aaatatcagc tgaataaaaa ccacttgaat cggactttcc
    21601 gctaaaaaat atccgtacga taagtagagt gccttgaaga tcggcaaaaa actgccggac
    21661 tttttagagg ctgtgacaaa gattttgtca cagcctcttt ttccatataa acggcgttcg
    21721 cctaccagct gcctggtaaa tctgtttcct cttcgttaag aagcgaaaat ccgtcaaatg
    21781 tttcgccatc ataataacga agcagctcat ccatggacaa gttcatcgtt ttttcttttt
    21841 cttcttccgc tgattcagcc agtagctgtt cccgatacac ctgatacata ccagctattt
    21901 tttgctgcat cgcttttcta gtttcacctt ccgcattgat tattggcaat tctttgatat
    21961 agccacgata aatcaaccga tcatctgaaa attcaatggg taattcaacg agtgtcaacc
    22021 cgacttcctg aaaatattct tccagcatct attatccccc cattcttttt cttaaatgaa
    22081 ttttagctta cctactgaaa attttccagt ttcattattt tgagaaaaaa agggaatgtg
    22141 acaaaaatcg tgtcacatcc cctttttccg aataaatgat gttcttctgc caactcctca
    22201 atcataagtc acaatattca acaaacatga gaaactgttt tttgaaattg ttccttgtga
    22261 ttcagagtta aacgacagtc tcacaacctc ttggtaaacg gcgttcaatc agctgaccct
    22321 cggtattagc tcaaaaaacg gtaaaatatg agaagctatt tttccgtttt gtttctctaa
    22381 tacttaggat aaaacgctga ttttacaacc tctgttaaac ggtgttcaat cagctgtcct
    22441 tcggtattag ctcaaaaaac ggcaaaatat gaggaactat ttttccgttt gtttctctaa
    22501 tactcaggac aaaacgctga ttttacaacc tcctgataaa cggtgttcaa aagcttgcat
    22561 ctgcggtaat aagcccaacc aaacgaaaaa tatgagaagc tatttttgtt tggctgttct
    22621 tatgacttga tgcaggacag ctttttcaca acctctgatt tcttattctt cttcatttgt
    22681 agcaaaataa ttacggtgat aaatgataca acatacactg ataagaacaa tggcacctgc
    22741 aaaataaaac gcatagcttt caccaatatg ttcaccaatc acacctgcaa taaatggtgc
    22801 taaagctcca ccgaaaaagc ggacaaaact atagctggac gaagcaacgt ttcgttgcag
    22861 acctggtatt tccatagcaa tcgtcgtcaa cagtgtattg ataattcctt ggaaaaatcc
    22921 tgctaacaca atgctgatcg gtacaaggac tggcatagaa gttccagctc ctaataacag
    22981 caaacaaata atgaataaga caaatgctgc taaaattgtc cgataagtcg taaacttccg
    23041 ctcaagagcc ggtgcaacga aaatcgaagc aatagccaga agcacacccc agccaaaaaa
    23101 tacaaaaccg acttctaatt cagaatagcc aatcaataaa aatggcgcat acgcaagtaa
    23161 agtaaagaat ccgaaattat ataacaatgc gattagtcca actgaacgaa gtttatgatt
    23221 gcttaacgct ttgacacctt cccaaaggcc tacttttctc tttggtttct cagtttcagg
    23281 taaaagtacc gctagggcta taaaaccgca aaacatcaaa aatgctaccc caaaaaatgg
    23341 ataacgccat gaaccactgc ccaagacccc accgactaga gggccaacag ccattccgca
    23401 gccaagtgct gcttcataca tcatgattgc tttttctgtg ttaccaacta aaatactgac
    23461 gatcgttgct aaagcagtag agacaaataa agcattcccg acaccccagc cggcacgtaa
    23521 cccaatcagc attccgatat tcccagataa tccgccaaga ccagcgaata caacgatgat
    23581 aaacaatcct gtcatcaatg tttttttcaa accgatccga ctagataaga aacctgtaaa
    23641 tagcatcacc acaccagtga ctaacatgta actagtaaat aacagggtcg tttcagctgg
    23701 cgtagcgttt aactgtgcag caatcgtttt taagattggg tctactagac ccactcccat
    23761 aaaagcgatc atacaggtag ctgctacaac tagcgctgaa cttttcccct ttgtttcttc
    23821 atttgtttgt tcttgtatat ctatttgatt ctccaatatt tccatccctt tctctctatt
    23881 agcatcatac atgatttatt ttacatgtca atatgcacat gtaaaacgtg gaaactttac
    23941 tgtgatataa taaagaaaat aagacgagag gtaggatttt tatgaatact ttagcctata
    24001 tattattatg cgtgctttta tccaaaccac gttctgggta tgaattgaag cagctgatca
    24061 caattttttg ggaagcccat cacagtcaga tctatacaac gcttgccaag ttagaccagc
    24121 agggttatgt gaccgttttg gacaaagacg agcactctca aaaaaagatc taccacttga
    24181 ctgatgaagg acgtgctctt gtagaggaat ggatcaaaga ggaaactccc gctcctacgc
    24241 aaaaagatga atttctagca aaaatctatg ctttttcgac gctcgataaa acaacagcta
    24301 ctggactgct attcactaga gaacaacagc tgaacaaaat catgcaaaaa aatcaaaaaa
    24361 aattgaatga cctctcgcta gacaagaaac aagaatttgg acgttatgtt gtgatccagc
    24421 gcagaattat gctttgtcag caggaactcc aatggtgtca gcaagtaaga gaacaaatgc
    24481 agcagttttt tgagtaaggg ataaacagaa cctttctaga aatatctatt gaagagcaaa
    24541 ttatcttcaa taaaagagca atacagttgg aaaagaaaaa gcgatccgaa cgacaggcca
    24601 aaacctatcg ttcggatcac tatttttcta gaactgcatt caagagacta tgacaaaaag
    24661 catgccaagg ccttttttca aataacagtg ttccatcatt cattactttg aattgctttt
    24721 agcacacttc ttgaaagcgg gccaacgatg atcaactgca acggtaaagc ggcaatcaca
    24781 ttcattttcc aagcattcat ataagcagag aataattgat caggtattcc ttgttccatg
    24841 atcacaccga ataaagacat acatgtcacc attcctaaaa ccatcaagca tgaaatagtt
    24901 aagatcattt ggaccttgct ttctggattt actggtagtt taaaggcaat ttttttcgcc
    24961 agcacaccga tgatgaagac atccaaaatg aaagccacca cgaatccagg cactagacca
    25021 atcaacaaac ttgataaatg aagatcttga tgcagccata aattatacgc gctcatcccc
    25081 aagaccatca aaaaacacat cattgttgta aaaattattc cttctttttt atttgtaggc
    25141 aatctgtacc actcctttaa atttgtcacg aaaatcatcg tagcacattt aaaaatttct
    25201 cgtaagtact ttttttattt tctgcccttt tatttcataa acttcatcag ccgtacgctc
    25261 gacaaagtaa gaatcatgag aagtaaacaa aatcgttccc ggatatattt gcattagctt
    25321 ctctaaagct tcgatcgttg ccatatcaat aaaattagtc ggttcatcta acagcaacac
    25381 attacttggc ttagtaaaaa gcaaagcaat cgtcagacgg gtcgcttctc ccccacttaa
    25441 tgtacataac ggtttcgtca aagtttcctg agcaaaccct aagtgattca gcaaactacg
    25501 gacaagcgat tctgaaaact ctgtttgtct catgacaaaa cgaatgatgg attcttcaga
    25561 tgtcatttga taaccctttt gctgatatac ttgaaagctt actttcggag agagaaggat
    25621 tccttctcct tgtttgcgga tatgttctaa taaggttgtc ttacctgaac cattttcacc
    25681 gacaatcgcg atttttttac ctaaagaaaa ctgaaaatca ccatttacca gtaatgttct
    25741 cccacttctt tccaattgga cattttgtcc catgatggga tagcggctgt ggagttcaag
    25801 agattttggt actggaaaac ggatttgttt catctgctgc ggcttggttg tttctcctat
    25861 tttttggagt cttctctcta atgttttcgc attcttttgg atggcttttt gtacactgtc
    25921 tttttgttta gaggaagaca accgatccgg tctgatttgt tgctttttct ttttactgga
    25981 cactttttcc aacttttccg cttgtttttt cttttcttgg atcgattttt ccaatcgttt
    26041 tttctctttc atgatcgact ctgcttctcg tagctgagtc tttttctcca attccttttg
    26101 agtgagatag gcactataat tccccgaaaa ctctcggatt tctccgtctt ttacttccca
    26161 gattttcgat gctagctcat ctagaaaata gcgatcatga ctaacaaaaa ccagcgtacc
    26221 ataataataa cgaagctctt caatcagtcg atcaatactt tttctatcta gatgggttgt
    26281 tggctcgtct agaagcatcc cttgttcata agaagaaaga agacgtatca actcgatctt
    26341 tcgttcttcc cctccgctcc atttccgctc tgaagtttct tccagtctta actggaaatg
    26401 actggctaat tccaaattta ggtcctttgc ttcagcgaga taggttaatt gaggcaggta
    26461 gttgaaggtg atctctgttt ggattttccc atgatcggga aaaagttcac cggctattag
    26521 tttgagcaaa gtcgatttac cgacaccatt ttctcctatg atccctatac gatcttgctg
    26581 atagacacga agagaatcaa tagatagttc tagctggttg ccaaagctga cttgtagatt
    26641 tgttatattt actgctaaat tttccatatg ttttacactc ctatttcatg taaaacaaca
    26701 attatcgata ctaaaaaaga gcagaaaaat ctgtttgcaa aaaaactgca tcacatcttt
    26761 tcctactctg aacgaaataa ctatgactaa atcaccaaat ctgtcatagc tttcacctat
    26821 tctactgtcg ttcgaaaaaa tggacatact ccatccatga aattgttgtt tatacatgtc
    26881 ttttgaattt atttagttgt tattcaaaag ttcgaaacgt aatttcatcg atgcagtcat
    26941 ttttccgacc tcctttttta ttaggttacc acacacgatt caaaaaaagc aatagctact
    27001 gaatagtaac tgaaaacctt ttattcgaac gtagtgatct tttctggtgc taaacgaaca
    27061 gactcgtaac cttcttctac cgcttttcct attgataaaa taataactgg aacataacgg
    27121 tctttatcca aaccaaatgc ctctgccaac tgatctgctt cgaatccacc aattggattt
    27181 gtttcatagc catgatcacg cgcaactaac atgaattgca tagccgctaa gctagaatcg
    27241 actttgacta catcgttcat ttcttctctt gaaaaactct tgtaataagg aatgatcgca
    27301 gctaattgtt gatctctgac ttcttttggc atcttccctg attcgtaagc ttggttgtag
    27361 atttcttcgc catattcgta acattgcata tcgccaaaaa tcatgaccat tgcagaagaa
    27421 gtatcatttt gtctcgtatt gaaacggatc aaaggacgca agatttcttt tgcttcctcg
    27481 ctttcagcga ccacaaagcg ccatggttgc atattgacag aagaaggtgc agtcgtcgct
    27541 tctttgatca tttccagcat ttcttcgtgt gggattttgt atgtttcatc atatacgcga
    27601 acggatcttc taccagtcat aattgttgaa aaatcatttt tttgttccat tattattaac
    27661 ctctttcgtt tctgttgtta tattgcaaag ttgggtattc aatttatcta aaagtgttag
    27721 aaattgtttc atttctacat cgctgaaagc cggggatagc acggaacctg tatctttatg
    27781 gtccatttca caatgcttga ttttcttctg ggctttttct gttagcgcta catacacttc
    27841 ccggttgttc tgaggatttc tctgacgcga aacatagcca tttgcttcca ataccttcag
    27901 atgacgagta actgctcctt gatcaatctg caacttttct tgcaaactgc tttgtagcaa
    27961 tggattttct tctttcagtc tcatcatgat ctcgtatcta gtaagactta taccgatatt
    28021 ttcttcaaag atctggacga ttttttgatc ggctaatttg atttgatata aaaggtgact
    28081 aatctcttct aaagacataa tttcctcctt tctaataatt gactcgtcaa cgattgactt
    28141 gtcaataata gaataaaaaa agaaaaaagt caaagaatca gctgtgtaat aaaaaaaacg
    28201 ctctcaatag aaaagtatag agaaaagaaa ttttttgtag aatgaacgga aaaaatgata
    28261 aagaaagcac aacgctaaaa ccaagattat tgattcttat caaactatag aagatagctc
    28321 ttctgcagta gaaaatttta caccgccaaa aatttccgaa atcgcctttt gcaagaatcc
    28381 cgtcgaatat tagaaattgg atcagtatgc ttaaacagta ataaaaaagt tatgacaaga
    28441 tgttgtcaca accttttttg agtaaacggt gttcaaaaac ttgcatctgc ggtaataagt
    28501 tcaaccaaac aaaaaacatg aaaatctgtt tttgtttggc tgttcttact acttgatgca
    28561 gggcagcttt ttcactacct ctttttaata accataaaat cctattcctt catatctcca
    28621 gcccatcggt gttagccaat ctctttcctt agcactcaag gtatagtgat gggtaccagt
    28681 tttttcattt ggattaaata gtcgataaat tggcttggaa ccaccagaat aaaaacttag
    28741 accttcataa cgccaaccga tttttgttaa agagttgatc tcagattgtg ccattgtgta
    28801 atgatggtct ccactgtaag gattgtataa acggtagaca ggttgtccgc tgtttggtgc
    28861 ttgccaagca atcccttcat attcccagcc tacagttggc agataatttt tttctccggc
    28921 attcaatgta tataaatgtt ctcctgtatg acggttatac agacgataca tcggagaagt
    28981 tgaagttggt gctgtatcat actgtgtcag attataggtt tcgattacat tgttcaatgt
    29041 agaaccataa ttaggcgcag tggcataacg acctgttagc caagccgtag catctttgta
    29101 agagttcgta ttgcttttcc atgcaccgga atagtagtaa acgcctggct gaaaagaagt
    29161 cgtcttcaat accgttacgt tatcttgcaa tgattctttg tacgaaggat attttctaaa
    29221 agcggcattc accgtaatcc attgcccatt cacatattcc gaagtaggca tcgtaacaga
    29281 ttggccatta tagcttcctt taatcccaaa tagattgtaa ttaggggcag aagccaaagt
    29341 actgttcccc cagccacttt caacgatggc ttgagcaatc atgactgatg catataagtc
    29401 attggcactt gccaaacttt gtgcagttga ggcaatacta tcaatgaacg cttgttgaga
    29461 attagcagat gagcgagcat aaaaagcata gccagtatag ttttcgaatt cagacaaatc
    29521 tgcttgatgg gtatgtgtca atgtttgccc atctgtcaat tggttcgtga tttcttctgc
    29581 attctctgga ttgttctgag cttctgctga tgtactgtct tcggtttgtg tttgttctgt
    29641 tgtttcagtt gttggttctt cggttgaaat tgtattttct gagacagtag ctgactgcaa
    29701 agtactttct gtatcgtttt caattcctgt ttctgaagaa ctcgatgtct gagttgcgac
    29761 agtttctgtc gattcttcag ctagcactgt tgtgttggca actgctagat ttcctaaaag
    29821 aataacgcta aataataaat tctttgcttt catctttttt ctcctttgaa ttatgaaact
    29881 tttcctaaat aacagtgtaa ctaaaaaagt aaaagcaagt caatgtccat atttaaataa
    29941 catttgtttc tttttaataa atttgctttt tctgcataaa aagacgtaaa tgaagaatat
    30001 tcttctccat tcacgcctta aattgatggt cgtttgaata actatttaac aagtaattct
    30061 ctctggatat tatccgttat ggattatact tcctgtgtcg tcggacggat aatcatgttg
    30121 ctgacagaca tccggtctgg cgtttctatc gcaaatgcta cagcagaagc tatatcttca
    30181 gatgttaatc cccattcttt ttgcgcttca tgaagttcaa gagcagcctt tttgtcgctg
    30241 atggtttgat ataattcagt ttgaacagct cctggagaga tgatcgtaga tttgatgtta
    30301 ttttgccgtt gctcttgacg taatccttcc atgattgcac ggacagcaaa ttttgttccg
    30361 caataaacgg cggaatcagg ataaacaaca tgtcccgcca cagaatcagt tgaaatgata
    30421 tgcccagatt tttgtttttc catgattgga agtacggcgg aaattccatt taacacaccc
    30481 atgatgttga tatcaagcat attttgccat tcatcacggt gaccttcaac taatggagcg
    30541 gttggcatga ttcctgcatt attgtacaat acatcgattc gaccgaactt ttccatcgct
    30601 aaatcgataa ctgcttggac ttgtgcaaaa tctcttacat ctgctgtttg aatatatagc
    30661 tcagcttctg gtaaagattc ttttattgct tttaggcgat cttctcggcg tgcagctata
    30721 actagtttag cccctttttc tgctaatagc ttcgttgttg cttcaccgat tccgcttgaa
    30781 gcgcccatga tgacaatcac tttttctgat aaatcattca ttctattctc ctcctgtttt
    30841 gaaaaatctc ttgcttatac tcactatctc ttatttcatc ttactgttca aataatccgt
    30901 tcgacaggtt tgacgatttt ggcagggatt cctgcaacga tggtattgtc tgggacatct
    30961 ttcgtaacag ttgcattcgc tgcaacaatg gcattctcgc caatcgttac gcctggcaga
    31021 atagttgctc ctgctccgat ccaagcattt ttcttgatgt ggattggctt tactgacaag
    31081 cctcttcgtt cttttggatc ggttagatgg ttcaccgtga tgaggttgac acgaggacca
    31141 atcaatactg aatcttctaa acagatgcca cctaagtcaa cgaacatcac ttgacgattg
    31201 ataaagacat ttttccctat actaatatgt ttgccgaaat ctgtataaaa aggcagcaga
    31261 acagtcaccg tatcatctac tttgtcacta atgatatttt ctagccgttt gcggatatct
    31321 gctggttcat ggtacccagt attcagcgct gctaaaggct cttcgttttc cgcttggatc
    31381 tgatggatca cttcaaagag cggatccgtt actcgaatct ctttattgac gatttgtttt
    31441 tcgatgaggc ttgtcatttt gctccctcct tttatcctta ctttataacg gacggcttat
    31501 gatgtctaat acttataaaa catacttaac tattcctttt aactatactt agtggtatgc
    31561 ttttttaaaa acaggaggga aaaaatggat attcgtatac tgacgtattt tttagcagta
    31621 gctagagaaa aaaatatcag ccgagcagca aaatctctgc atatcacgca acctactctc
    31681 tcaaaacaac tgaaagattt agaagaagaa cttggtgtga cacttttcac tcgaggctcc
    31741 cgagagatac agttgacgga ttctggtcga tatctttatt caaaaggaaa agaaatcttg
    31801 tcgttggtag aaaaaacggc gaacaattta acaaatgacg caatcatctc tggagaaatc
    31861 tacattggcg gaggcgaaac taaagcaatc tctttcattt ctgatagcct tcattcgttg
    31921 atcaaggcgc atcctgatat ccagtttcat ttatatagcg ggaatgcaga tgatatcata
    31981 gacaaactgg atagcggatt acttgatttt ggactcgtaa tcgaaccgac aaacaaacaa
    32041 aaataccagt catttcgact gccagcccaa gatcgttggg gacttttgat acatcggaat
    32101 catcctttag caaagcaaac agtcatccat ccgcaggaac tgaaaactgt tcctttgatc
    32161 atttcccggc aaacgtcagt ggacagtcaa ctagctgaat ggctaggttt cagcatagat
    32221 cagttatcga tcgtcggaac atataactta ttgtacaatg cttctttgat ggtcgataac
    32281 gggacttttg gtgcattagc tctagatggg atcatcaata cttatgggac ggaaaaagta
    32341 ttcatcccac tcgatccgcc attacaagct tcactgaatc tcatttggaa aaaagaccag
    32401 cctctttctc ctgctgctcg taaattttta gatgtcctaa cggagaaata cagttaagta
    32461 gagttttttt ccttcgcttt cagcttttta acaacgaata agttaagcaa aagatccaat
    32521 gattaaataa cataagtttc ttaatttgtt ccttcctttt atttctgata attccagtaa
    32581 attactataa ttataaaaaa agaattgaca aaacataact gtctatataa aattgattac
    32641 gttaacaata attcgtttca tgtgactaga tcatactagg catgaaaaat ctctgaggaa
    32701 catggtttcc ctaggaggtt tttcgtgtct atttttgttt ggccgcaatc aactggtcta
    32761 gcaaacgtga aaaccaaatc ttcaaaaagg gtgcgcgcat gaaaatcaaa aagatcttta
    32821 atcaaaatgc agtattggta gatgacggtg gacaggaaaa agtagctatt ggaaaaggga
    32881 ttggatttga cagaaaaaga aatgatttga tttttgatcg tgatgtcgaa cggatatttg
    32941 tcatggaacc agaaggtcaa atcaagttgc aaaatttact taatcagatt gatgaacgct
    33001 ttttatttgc agcagaaacg atcattgatc atgcggaaac tgttttgatg gaaaagttga
    33061 atgaacatgt attgatcgct ttgaccgacc acattgcttt ttctgcggag aacattaata
    33121 acggtatcgt catccgcaac aaacttttaa gagaaatcga agtgctttac agtgatgaat
    33181 tcagtattgc tcaatgggca gtagagtatc tgacaaaaga gcttgggatt ccatatactt
    33241 atgacgaagc aggatatatc gcaatccata ttcacagcgg acgaagcggt cggaacaata
    33301 accatcgcag tatccgtgaa gtgacgattg tatctgacat catccattta gtggaatcag
    33361 aattggaaac agatatccat tcagaaccat tttcactgaa ttattcaaga ctggtgaatc
    33421 atttacgact gcttctacaa cgaacccatg cgcaacaata tgctgtttta gacacggaaa
    33481 tcgtagaaat ggtcaaacga aaatacccag aaagctataa gatttctaaa aaaatccgtg
    33541 tgttgctgac caaagaatac cagctcgcga tcacgaaaga agaactgggt tacttggcca
    33601 tccatatcga gcgtttaaga agcgcaatcg tacaaacaaa tgttaacaat catgaggagg
    33661 aaaaaaattg aaagcatatc tacaacgaat gggacgttcc cttcaacttc cagttgcggt
    33721 cttacctgct gctgctttac tagtagggat cggaaactgg tgggcatctt atagtaacga
    33781 cattgtcgct cattttttac aagctggcgg taacgcagtt ttaggtcaat tacctttgct
    33841 gtttgccgtt gggttagcac tagggatgtc agtggacaaa tcaggtgctg ctgccttggc
    33901 aggtttggtc gcatttgaag ttccagtaaa tgttttaaaa actgattcag tagctacttt
    33961 attgaatatc aaaccagaat cagttgatcc atcttttgct cagatcagta acgtatttat
    34021 tggtattatt gccggattga ttgcggcagc tctttataat cgtttccacg aagcaaaatt
    34081 accgatggct ttatctttct ttagcggcaa gcgtctagtg ccaatcttgg ctgcctttgt
    34141 catgttgctg atcagtgcag tgctactgct cgtatggcct ccggtttact ctgctttagt
    34201 ttcttttgct aaattcattt tgagtttagg ctggggtggt gcaggtcttt atggcttctt
    34261 caaccgttta ctgatcccta caggattgca tcaagcaatg aactccgtgt tctggtttga
    34321 cgtagcagga atcaatgata ttggtaaatt tttggcttct gaaggaacaa aaggcgtaac
    34381 aggaatgtat caagctggat ttttcccaat catgatgttc gggcttccag caggagcata
    34441 tgctatttac agaaatgcac gtccagaaaa caaagcaaaa acagcttcct tgatgttagc
    34501 agcagctttt gcttcattct ttactggtgt aacagaacca cttgaattct catttatgtt
    34561 cgttgcttgg ccattatatg tcttgcatgc aattttcact gggatttctt tagcagtgag
    34621 tgcattcttc cattggacag caggatttgc ctttagtgcc ggatttgtcg attacttcct
    34681 atctttgaaa aatccgattg cgaatcagcc attgatgttg atcgtccaag gattagtaac
    34741 agcagtgatt tactactttg gctttaactt tgctatcaaa aaattccatc tgatgactcc
    34801 tggccgcgag gaagcagatc tttcagatga cgatactgca acgactaatt cagacaataa
    34861 atatgcggca caagcaagca aaatctacgc tgcattaggc ggagccgaca atgtaacttc
    34921 gatcgataac tgtactacac gccttcgttt gcaagtcaaa gatacaggcc tgatcgatca
    34981 aaataaaatc aaagccactg gcgtacctgg tatgaagatc atcgacggaa aaaatgcgca
    35041 agtcatcgta ggtacagaag tacaattcgt tgcagatgag atggcaaaac tacatggtgg
    35101 tgctgcaaca cgacctgcac aaacaaacac cgtggttaaa acagaaacat ttgccggtgg
    35161 aacgatcgaa caagatattt acgctatcgc aaatgggaaa ctgattccaa tcactgaagt
    35221 ttcagatgat gtattcgcgg aaaaaatgat gggtgacggc tatgctgtat taccagaaaa
    35281 tggagaaatc ttctctccaa tcgctggtac gatcacaaac attttcccaa cgaaacatgc
    35341 tgtcggaatc caaacagatg ctggtatcga agtcttgctt catatgggca tcaacactgt
    35401 tgacttaaaa ggcgaaccat tcactttata tgtaaaagaa ggtcaaaaag tcgcaagagg
    35461 tcaattgatt gctttagttg atcttgccgc tattcaatct gcaggcaaaa acacggatat
    35521 ggtcgttgta ttcacaaatg gcgacaaagt ccaaagctta gaaatcgaac ccgcacgtga
    35581 tgtaaaagca aatgataaaa tcggctctgt ttccaacaaa gcctgagcca tcgtcttccc
    35641 cagacttttt tatcgataaa caaacaggag ctgagacaaa aatcttgtct cagctccttt
    35701 ttccgaataa aaacggcgtt ctcctgccaa ctccgcggta ataagtccag ccaaacgtaa
    35761 catatgaaga gctatttttt atttgactct tcttattact tgatgcagaa cagctttttt
    35821 acaacctctg ataaacggtg ttcaaaaacc agtttatcgg tatgaagtca aatttatgca
    35881 aaaatgtgga aagccatttt cacaaaattt ttcttcatac tcaaaactaa tcggcttttt
    35941 cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc ttcaagagaa
    36001 gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa ctgatccttt
    36061 ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa tccggcttct
    36121 tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactt tttacctgtc
    36181 gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga taattctaag
    36241 atttctggag agtcactttc tactagtact tttgctgcag cttctgcata ttcacgtttc
    36301 aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt ttttaaagcc
    36361 tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa tgtatgggaa
    36421 atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa tacactgatc
    36481 gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc tgcttgtgct
    36541 gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga gacaaaaagc
    36601 aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata atcaccaata
    36661 cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg tactagtaca
    36721 tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc accaaaacct
    36781 ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt ttatttgtta
    36841 agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg gtaagataag
    36901 tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat ttagtgatgc
    36961 aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt ctagtgagat
    37021 gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga gtcagctcaa
    37081 aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg ccaaaagtcc
    37141 agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac agcttatcca
    37201 agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag aagctttaga
    37261 agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga tcacacttga
    37321 tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa acacaggcct
    37381 cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc ggaatcgtct
    37441 acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt ttcctctcta
    37501 atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca agtagctgac
    37561 cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt tttcctctct
    37621 aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt attgactacc
    37681 ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac tacagcattt
    37741 ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa ggccgtattc
    37801 gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgattacaac aagatacgtt
    37861 ccttttggga atgggatgat tcgggtagtg tttggaacat cctgactcgt ttgaacaccg
    37921 acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata atgattttca
    37981 gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc aaatgcttgt
    38041 ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac accaataccc
    38101 gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc caccctttct
    38161 attatattaa gttcatctta cataaaatta aagggaaatg acacgattac gctctttttt
    38221 ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca aatctcactg
    38281 taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc ttcgactaac
    38341 tcataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc ttgtaaagtt
    38401 tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat agcaagcatt
    38461 tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg catcaaactg
    38521 cccttttctt ccaagcgggc tccatcaaat ccataagaaa cgttgattac ttgatttgga
    38581 tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt ctgcgctaac
    38641 ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc cacacctgcc
    38701 acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc tctgacatca
    38761 ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctgctgat ttctgaaggc
    38821 agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc atatccatat
    38881 ctaaacgccg tttctgtcac tcttgatcca tctaggagat cgcgattagc caaagccaat
    38941 ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa gatgcgtttt
    39001 aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag tccttctgtc
    39061 aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt tatcttcctc
    39121 cttgcttgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa tcaggaacca
    39181 atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag cagcgtttcg
    39241 taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa taaattttgg
    39301 ggaagacaca ttctaagtaa acaaagcggt aaaaatatct tttattccct tagttaatcc
    39361 gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga acgctgcttg
    39421 gatttttctt tgcgatacat catacctctt ttatcaattg gaacaattat ttatttctaa
    39481 caaaatcact tcatttttgt cagataaata aaaaacaaaa aaagttccat ctttaaaaca
    39541 cgctattctt ttactttttc tcacattctc taagaaaaga aaaggagaat gaatttttga
    39601 caattcgagc aaaagtgatt atcaaatatg taaaatcagc tacttttttc ttaaacgttt
    39661 ctttatacta aatataaata agtgtttcat gaaaggagaa ctagatgaat aattggcttg
    39721 agtattttcc tgaaaacgtt ttggaacgcg gttacagcta tcatctgcac ggttttgtcc
    39781 gtcatttgaa ctatacaagc aagtacttgt ccgcaacagt ttctggcacg gaagattata
    39841 aggtagtgat cacttgggat gaaaagacca acatgacttg tgattgtcta tatgccatag
    39901 aaggaaaaaa atgcaaacac atggctgctg tcttattcgc ttacgaagaa cgtccaataa
    39961 aaaaatccaa ctactcactt tccgaactct cgtcccttgt ttcctccgct agttcctctc
    40021 ttgtacggga gcttttaaca gagattctta tagaacatcc gcaatttatc gaacgcttca
    40081 aagttaagat gccatttcat gcaataaatt attcagataa attaacaact atcatccata
    40141 aatacgatca tattatcaag aaaaacaaaa acaggaaaac agcgaagttc atcatggaga
    40201 tgcgaaagtt catccaagaa gcagttgaat cgctcattca gcaaaatgcc tatcttcccg
    40261 cattcgaatt gatcaatgaa gtgattgcta ctctcgaaac attctattgg gagcctgagg
    40321 atgaacgaac ccttcttctg atagaggact gttattattt atggaaagaa ttattagcag
    40381 aagcacctca tgcagaaaaa aggcaaatgt tctcttggtt cgtctgccaa gtcgatcata
    40441 ctgacgcctc ttattcaaaa cgctactcca taaaaatcct gaaagaagac tttcgggaaa
    40501 gagaattttc taaccaaaag aaaaaaatag ataaaaaaac taaagaaacg gtaaagaaag
    40561 acgatttcaa tgaaaaatag caagtaaacg ataacaacca tcctaaatcc acaagaaata
    40621 cagcagacct tgctttaaaa acacgaatca tgcaaaggat gaagtaaaga agacttaacg
    40681 cgtacccaat attagtcatt tgactattag gtacgcgttt tgttttataa gtcatgacaa
    40741 atttccttcg ttgctgggta gattttccgg cattgttgat agattttatg atacttttct
    40801 acattttgta agagaggttc cactgcatct gtatattgga taaaattctg acaacaactt
    40861 tcaggtgtgt cgaaccactc taatctttaa agataaatat taaaataacg attaataata
    40921 ttaacagtct ttatttttca ccaccaaaaa cctatctttg catatactaa atagacgtat
    40981 atacacagat aggtttgtct tgtgaaaaat aatgattatc cttaccaaaa aatgtttgtt
    41041 tttctacatg ttcgtactta tcaaaaaata tgatccttat tgaaattact ggctatttat
    41101 attccaacga cttcttgtgt taatttttgt tatctcatag taaattatgt ttcattttca
    41161 cttaatttag ttgtcacgtg ttcgtcaatt cgtaaaaacg gtaaatatat aaacacactt
    41221 gcggctataa ttaatatttg taaaacagct gctcgccagt ctccggctgt tgctaaaaat
    41281 cccgatataa ctggtggcgt agtccaagga atttgcacta ctacatgatt aattaatccc
    41341 acagcagttg caaaatatgc tactgtcaaa gaaaaaatcg gtgcaagcac aaagggaata
    41401 atcagactga tattaaatac aataggcaat ccaaaaataa taggttcgct tatattgaat
    41461 atagcgggag ttaccgataa tttagcaaag tccttataat cttttctacg actgaagata
    41521 aatattgcaa tcaataaagc aatcgtattt cccgatccac ccattaccgc aaaggtatct
    41581 ttaaaggcca tattgataat atgtggaatt tgctctttgt ttgcataagc tgccatattt
    41641 tcttgcatat tctgaatcaa aaacggatct aacaatgaac ctgatattac tgcttgatgg
    41701 ataccaaatc caaaaaatag attagcaata gatgtaatta acaaaaatcc tggtaaacca
    41761 gttgtaattt tacttaatgg ctctgtaact atcgtggtaa ttagcttatt tatatccata
    41821 ttaaacaatt gaatgattat aaatgacaat aaagcaaacg aggtaatcgt aataatgatt
    41881 gggattaata cattaaaaga ctttaaaaca gcgggaggaa tatttccgga tagctgaatc
    41941 tgtagtttct tattattaga aagtttaata aataattctg tagccgctaa tccaacaaat
    42001 atacctacaa acattcctga agctcctgta taggatatag gtatcacacc cgtcacttca
    42061 acagctttat ccgcattttc aggagtaaac atcatctgca taggtgttaa tacaacaaat
    42121 gtcgaaatag caactaatac cgctgcaata tgatttgtat aatttctgct tgcacacata
    42181 tgataagata ctgctgctac aactaataat gtagttatgc ttaatgtacc attggcaatt
    42241 gaagtaccaa tttgttgtaa cgtggtcaat gtactagtat cgacaatttc ccccatgaat
    42301 cctgttggtt ctaagatcac tgaattaata agtgtcacaa atcctgctaa catcataaat
    42361 ggcataaatg tagcaaacgc atctcgcaaa gttcttaaat ggatctgatt ccccaactta
    42421 gtcgcaacta taccaaattt ttcaaataca atgtccttcc tgctactttt gcccatattt
    42481 tttctccttt atgcttattg taatcgatta ctttttccac ccaaataata catcacttat
    42541 caaaaaaagt aa
//
LOCUS       DAZKCY010000011        41804 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000011 DAZKCY010000000
VERSION     DAZKCY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.011"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(193..1503)
                     /locus_tag="ACG8XC_000544"
     CDS             complement(193..1503)
                     /locus_tag="ACG8XC_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFP9190267.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1515..2933)
                     /locus_tag="ACG8XC_000545"
     CDS             complement(1515..2933)
                     /locus_tag="ACG8XC_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9190268.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3173..4741)
                     /locus_tag="ACG8XC_000546"
     CDS             complement(3173..4741)
                     /locus_tag="ACG8XC_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFP9190269.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHYLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4745..5155)
                     /locus_tag="ACG8XC_000547"
     CDS             complement(4745..5155)
                     /locus_tag="ACG8XC_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFP9190270.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFVAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5152..6063)
                     /locus_tag="ACG8XC_000548"
     CDS             complement(5152..6063)
                     /locus_tag="ACG8XC_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFP9190271.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPKVLIVKEGRY
                     DEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVLENLH
                     IENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFCTPAS
                     ANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKNQKTG
                     YRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNEQTVK
                     FSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6060..6938)
                     /locus_tag="ACG8XC_000549"
     CDS             complement(6060..6938)
                     /locus_tag="ACG8XC_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFP9190272.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6925..8262)
                     /locus_tag="ACG8XC_000550"
     CDS             complement(6925..8262)
                     /locus_tag="ACG8XC_000550"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFP9190273.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMRRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8265..9368)
                     /locus_tag="ACG8XC_000551"
     CDS             complement(8265..9368)
                     /locus_tag="ACG8XC_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9190274.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9442..10950)
                     /locus_tag="ACG8XC_000552"
     CDS             complement(9442..10950)
                     /locus_tag="ACG8XC_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9190275.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10916..11608)
                     /locus_tag="ACG8XC_000553"
     CDS             complement(10916..11608)
                     /locus_tag="ACG8XC_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFP9190276.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11630..12604)
                     /locus_tag="ACG8XC_000554"
     CDS             complement(11630..12604)
                     /locus_tag="ACG8XC_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9190277.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12904..13776
                     /locus_tag="ACG8XC_000555"
     CDS             12904..13776
                     /locus_tag="ACG8XC_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9190278.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14358..16556)
                     /locus_tag="ACG8XC_000556"
     CDS             complement(14358..16556)
                     /locus_tag="ACG8XC_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9190279.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16788..17780
                     /locus_tag="ACG8XC_000557"
     CDS             16788..17780
                     /locus_tag="ACG8XC_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9190280.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17840..19261
                     /locus_tag="ACG8XC_000558"
     CDS             17840..19261
                     /locus_tag="ACG8XC_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP9190281.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19254..20753
                     /locus_tag="ACG8XC_000559"
     CDS             19254..20753
                     /locus_tag="ACG8XC_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFP9190282.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20792..22186
                     /gene="uxaC"
                     /locus_tag="ACG8XC_000560"
     CDS             20792..22186
                     /gene="uxaC"
                     /locus_tag="ACG8XC_000560"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9190283.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22345..23028
                     /locus_tag="ACG8XC_000561"
     CDS             22345..23028
                     /locus_tag="ACG8XC_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFP9190284.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELSQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23030..23866
                     /locus_tag="ACG8XC_000562"
     CDS             23030..23866
                     /locus_tag="ACG8XC_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9190285.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23877..25631
                     /locus_tag="ACG8XC_000563"
     CDS             23877..25631
                     /locus_tag="ACG8XC_000563"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190286.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFMTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25907..26248)
                     /locus_tag="ACG8XC_000564"
     CDS             complement(25907..26248)
                     /locus_tag="ACG8XC_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9190287.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26316..28487)
                     /locus_tag="ACG8XC_000565"
     CDS             complement(26316..28487)
                     /locus_tag="ACG8XC_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP9190288.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28698..29555
                     /locus_tag="ACG8XC_000566"
     CDS             28698..29555
                     /locus_tag="ACG8XC_000566"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9190289.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29626..30483)
                     /locus_tag="ACG8XC_000567"
     CDS             complement(29626..30483)
                     /locus_tag="ACG8XC_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9190290.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30468..33170)
                     /locus_tag="ACG8XC_000568"
     CDS             complement(30468..33170)
                     /locus_tag="ACG8XC_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP9190291.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33183..34472)
                     /locus_tag="ACG8XC_000569"
     CDS             complement(33183..34472)
                     /locus_tag="ACG8XC_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFP9190292.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34622..35668
                     /locus_tag="ACG8XC_000570"
     CDS             34622..35668
                     /locus_tag="ACG8XC_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9190293.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35670..37823
                     /locus_tag="ACG8XC_000571"
     CDS             35670..37823
                     /locus_tag="ACG8XC_000571"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFP9190294.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38296..39747)
                     /locus_tag="ACG8XC_000572"
     CDS             complement(38296..39747)
                     /locus_tag="ACG8XC_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9190295.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39744..41468)
                     /locus_tag="ACG8XC_000573"
     CDS             complement(39744..41468)
                     /locus_tag="ACG8XC_000573"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9190296.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSIAFKEG"
     gene            complement(41461..>41804)
                     /locus_tag="ACG8XC_000574"
     CDS             complement(41461..>41804)
                     /locus_tag="ACG8XC_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9190297.1"
                     /translation="YLSVQIKGLIFLAIDFILLFALLCLFATYQYSMILDSEYTISVP
                     NLLKLSFISVFSSFSSFLKIIIGSGIILGVTWQFKGLILFGVIGLLTVWNGTMTTHWR
                     EELDKQLESYE"
BASE COUNT        12295 a         8567 c         7381 g        13561 t
ORIGIN      
        1 cgatagaatc aagcttcgca agtggttctt tttaacgact tagttatctt atcacgtcta
       61 aaaacatgtg tcaactcttt tttggaattt tcataaaatg tttcaatgaa gcgcgacaac
      121 gttgtttaat atatcattaa aaaaaataaa ggtcaagcta aaatattatt taagcttgac
      181 cttattataa tttcaacgat tgtcaaaagt attgtttgca gctgtgttgc cagtttctat
      241 agataaatca cagcttttga aacataagtt ttgtactgct tcgattcgcc cgaattcttt
      301 tgcagttatt tggcaatgag aaaagcagac atcttctata ggcttttcag gaaatgctac
      361 taaatcaaaa gctcgggaag gaagtgagta acctggagaa aggcatgcct gtacgttttt
      421 gatgtgtatg tctttgactt tcgtcatttg ctttgattca ggtatttctt cagccactgc
      481 ttgccaactt tctggcattt gagcaaggtc attgctattt tttctattgt attcattatg
      541 ccagtccatt atccaagaaa aaggaaattg tacatcaatc atattcagat tttctaccct
      601 gatattttcg atcactcccc ctctttcttt tgaagatttc attcgaatac cacagtctga
      661 ctgaaaaaaa tcgatatcgt gaatatatac gtcactaact ccagcactaa cttcgctacc
      721 aatagttaca ccgtatcctg aacggatttt acatctagca acctcgattc ttgatgattt
      781 tcgattgact cttgcaccat tgccatctct gccagactta atagcaatac aatcatcacc
      841 acaggccaaa tcacattcat agacacgcac atttgttgaa gaatcaatat caatcccatc
      901 tgtacttggt ccatcattgt tgcggatgat tactttttct actaatacat cgttagaata
      961 agtgatttgc aagttccaaa aacctgattt ctgaaaaatc aaatcacgaa caacaacatg
     1021 ttctgcatga tacagtaaac aagcgcgtgg acgtttaata gcataatccg caatccaacg
     1081 aagtcccttt tgatcgtatt cagctcttgt cccgcttttt tgatcttttc cccaatacag
     1141 ctcccaccaa tggtctcctt gaccgtcgat tattcctttt ccttctataa gaatatcttt
     1201 cgccgagaga acattcaaaa tcgctgctgg ccatttcatt tctacgccgg ctactcgtgt
     1261 atcgatttct ggaaatgctt cgatatctgt tctccccttg aggactgcac cttcttcaaa
     1321 gataagatga ctcttatctt ttaaaaaaag ggctccgact aaataaatgc cttttggaat
     1381 aaatacaggt aagtgttctt tcgcaccttg atcgattgct ttttgcagta ttattgtatc
     1441 atcttgttga gtgtcgtttg ttttaaagtt caaagcattg ataatccctt tttttgtccg
     1501 caatacgttt ctccctatct ttcattgatt tttttcaatt cttcaccaat aaatacagtt
     1561 tttgaacgat tcagtgggta acctactagc aaaatgattg cggatacaaa taagcagaat
     1621 gctggtaaca agtttgccat cgtataaatc ctttcttgta ctaaaactga ttggatggct
     1681 ccacctgttg tagacgattg gtaaccaatg agtgccagca taaatccgcc taccgctcct
     1741 gcacatgctt gtcctacttt tctcgcaaaa gaatttactc catatactgt tccatcttct
     1801 cttaagcctg tcttatattg atgataatca atcacgtcag taatgaatgc ccagaccatc
     1861 aaattgaata aaccagatcc taaagtagcg ataaataaaa gaatcagata tacccatgca
     1921 tttgttatat gaaggaaata catcagcaag tacatacatg aagaagcaag taacgcaaat
     1981 acgcttgctt cctttttgcc aaataatttt gtcagctttg tagcaaatgg tgctaaaact
     2041 actactgttc cgtaagtaaa taaaagagca acagacattg cttgtttatt atggaagtaa
     2101 tcattgaata aataagtcat attcgtccca gctaaaatct gattgatcac aatgaaaatg
     2161 tcaacaatga ctaaaataat cagtgctcga ttagctaaca aaccttttac caactgagaa
     2221 gcagatactt tttcgttttt gacaactctt actctttcag tagtcaattt acaagttaac
     2281 atatatgcga caaatgctat tgcagcacat ccgattgaaa tccaaaagaa atgattccct
     2341 gaaacgactt gttgtccact cttgttatgg gtatacatta aaatcggaat gaagaatcct
     2401 gtagttgatc cgccaatcgc agaaccaatg cttcggaacg tggacaacga ggtgcggtca
     2461 ttaggatttg agctaattgc tgctgccatt gctccatacg ggatattgat tgttgagaga
     2521 aaaatcccat aaacaatgta tgaaacaaaa atataagcaa tcttcaccgt cattggaaga
     2581 tgttggacga atggcaaaaa caaaatgaca gtcactaagc aaaatggata cttcattttt
     2641 ctgatccaag gaataaatcg gccagtttct gttaactctg agacatcaca taatcttcca
     2701 accatcacat cagcaaacgc atccaaaatt ctcgaaatca agaataacgt acctacgatt
     2761 gcccctgaaa cgccaagaac attcgtataa taaatcatca agaaactatt gactaaacct
     2821 aaaataaaac agttacctag atcaccgaac atgtagccta tcttatcgga aatcccaaat
     2881 tccttttttt ctttttttac tgtatttgct gtaacagcat tttgtgaaag catttgaatt
     2941 cctcctacct cttctagtaa acgttttcaa aattataact ctttttttct tgtttttata
     3001 ttcacaatgt tggcttttta ttgcattatc ttgacagata aaaaacaaaa aataatctaa
     3061 aaacattgat gcataagatt gataactttt tttacataac aatatataca aacaaaaaaa
     3121 ttcactaact agtgaaaata agttagtgaa cctcatttat taaagaatta aattacagat
     3181 gatcttttcc atcatacttg aaaaactcaa aatcagcata acttcctctt tttttattta
     3241 gatcatgcac tgctatcccg ataaaatttc cagtaaaccc tcctgagagt atacacatgt
     3301 ttttttgcgg acctactttc ttccattctt cagttgatac gtctttcaaa tgaaactgtg
     3361 ctgtctcttt ctttacagtg actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc
     3421 aatcttctat ttgtgctact ccgttttctg attctatcat acgtaggcat tttcccttgt
     3481 cctcattcca agtaacataa caataaatat aattggattc atttaagtac agtaccagtc
     3541 cagccatctg attaaaatta ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa
     3601 aatccgtttg ccgaatagct aacaaatgat gttcgaaggt agattgaaga gattcaccgg
     3661 aaatcagacg taaaaaacct ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa
     3721 tgcgtctggc attccagttc ttctttagct ctccttcaaa tgtatccata aactgatgat
     3781 ctttttcttg ttgtacctct tcatttgtct caatcattat ttcttccatc ggagttgtct
     3841 ggctatcttt aagtcttaac caatcgtcgg acgtccaata cacttcttga atagcggttt
     3901 ctcttcccaa aatagccgca ccgtttaatg gtctggtaca aagatatgtc atgtaccatc
     3961 ttcctgacgg ggtttgtact aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt
     4021 tgtcactagc tgtcagcata ggaaaaccag gatcaagctc gtaaggacca gtaatattct
     4081 ttgctcggca aactgtaact gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat
     4141 aataataccc gttacgaaaa tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg
     4201 caaatatttt atagatggga ccatcaagct tcttcttctc tgcatcatat tcttgtaata
     4261 cgataccgct ggatttattc ggtgtgtcca gacgataatc ccatagttca ttgacaagcc
     4321 atttttttcc cgtttcaaga taatggaaca aagaaggatc aaagccgtta ctatttaaat
     4381 aaattggttc ggaccaagga cctgtgatct cagatgcagt gatcaaataa ttgtggacat
     4441 ctttaaacgg acggcctgca cttttgacat ctgtgtaaac aagataaaac aatccatcag
     4501 cataactgat ctgtggcgcc catatacttc catttgtagg atttccttgc aaagatactt
     4561 gagtggtcaa aatatccgtt gtatgtgacc agttgactaa atttttcgac tcatatactc
     4621 ggattcctgg aagccattca aaagaggaga caataatgta ataccgctct ttcactctta
     4681 atatattagg atctggattg aatcctctta aaataggatt tttcactttg atagtctgca
     4741 tgattcaggc tccttttctc attcttactc gatataaact gaatgcccca gctgcgaaat
     4801 aaaaaaatgc aaaaggaaac gcaacaaact gagtgccaat caacaatatg aatcctgtta
     4861 taaaatacaa accagtggca gttgttaagt attttccttt agcagtcaaa aacaatgcta
     4921 ctgctaaaaa agcaagtgaa ataaacaaca caacggcaaa cagaccagtc atgtcctgta
     4981 ataatccaaa tgtattttct aaagacaaag ttgaatgatc tacagcaagc gactgataac
     5041 tttttttaaa ctctgacgca gacatattac ttaaagtaac tgctaacccg ccaaagaaaa
     5101 tcaaagatag actgccaccg accattccta gcaatagttc ccattttctt ttcatttctg
     5161 aaccccctgc ttttctaaat gtatccattc aggtcttttg atcgatgttt cataattgtt
     5221 attacgttca gagaatttga cagtctgttc atttttcttg tcatcccaat tatcccaacc
     5281 agttgggtga acatgcccac ctatcttaca cgcttcaaat aagacatttg catacggacg
     5341 ccacggtctt cctaaataga cgtttctagt atcaggttct gcagtcaagg tacagctttc
     5401 aaaacgatac cctgtttttt gatttttcgg cgtagatgct gctgtgatgt atccaggatt
     5461 tttactggct aagctatgga tatgacactg atgaaaaacg gcatctgccc caccaaatat
     5521 gaaatccacc gttccttcaa tccagcaata ttcataggtc tgacgacaat actcaggatt
     5581 ggcagaagcc ggtgtacaaa aaggagtacc atctttttgc aaatcaggca acggacccgt
     5641 gcataaggta tcctgataac cagatagtcg acagtttttt aaagatacgc gatctcctga
     5701 attgaataaa gcaacagcct gtcctacctt ttctcctgga cctgctgtat tttcgatgtg
     5761 aagattttct aatacgacat tcgatccttc taagaaaaaa gtagcacttc ggaaagtccc
     5821 taactggcta ccgtcaagat actcctgttt tgcaaaacga ttaccggaga taactactgt
     5881 tcctgctccc acaagtttaa aattggataa tctgctttct atatattcat catatcttcc
     5941 ttcttttact atcaagacct ttggcaggcc tgccaaataa tcgattcctt cttggatagt
     6001 ttcaaaatcc gtcggggtat ctatagcatt ctttccaatt atcagtttgt ctttcatcct
     6061 cataaatgct cccttatgaa ttgtttccaa atatttctca tttcttgtgt ttccatatga
     6121 ccgcctgtta aagaaaaaga agaaaagttt tctggactac ccattgattg atactttttg
     6181 tccagccctt ttctcagctt cagtacgcct tcgtccaagc acatcgtatc gttttttcca
     6241 actaagctaa gacgagggcg cggagcgatt ttttcttgga ttgctaacgt tgtataagct
     6301 gtcaatagat ttggcacata gtaataaaac ccatgatgat ctaggcggcg atgtagcaaa
     6361 agtgtttcga tgtccacctg tgccgctaga tcaatacaaa ctttgacacg agaatccaat
     6421 gctgtcaccc accagcttaa taagcctccc atagaaagac caatcgttgc gagccgttga
     6481 gtatctgtgt cttcccttgt ctccaaataa tcgatcaagc tgatcacatc ataaatacgc
     6541 attccccata acgtttttcc tgttaataaa aattgtttga acaactcgct ttcagaaata
     6601 cctccgcgtt cttcaaatcc ccaagcatca atcgaccaaa cagcatatcc cattcctgtc
     6661 agtacttctg caaaagatgg agaagctaag taaggagcac catggatcaa ttcacttttc
     6721 ccgactgaaa aatctccgcc gtgagaatgc aggtaaagga ccgtcggaaa cggtccttct
     6781 ttttctggat aagcatagag ggctggtact gtttccaatt catttaagta caattgatag
     6841 tattcaagca taaaaccgtt ttgtttttct cggtataatc gttttccact aggggtatgt
     6901 ctgctagatg gagcacctaa aaaatcattc aactgcataa gcaaagtttt tacgaacaac
     6961 cagttcatcc atattttctg attgatttga ttggaatttc tcaatactga tttctttagt
     7021 attttctaat agaaatgctg gtccctcgtg attttcaatc gacatctggt taaaggaaac
     7081 cttacccgca aagccaatat aaaatccttg tcttctcatt tcctctattc ctgtcatcat
     7141 agcaggaaca ccgggaactg catcttgggc catcgagaca tcgatattgt caaatgtgat
     7201 ttcttgtatg aattgttccg ctaatccata aataaaacct gcagaagcat ggacattcct
     7261 tgccgtgata ttcgaaaaat ggatccttct gaatgctggt gtccgttcat ctatcggata
     7321 agcctttttt tcccatacat acggttcttt ccctcgcgga ccacagaaat aataaagatt
     7381 cagtataaat ggacacatca cattatccat gactatattg gatactcgaa tatcttcaac
     7441 gatcccgcct cgacctcgtc tagatttcaa tcgtattcct cgatcagttt cttggaagat
     7501 acaatttgaa atggtaatat tacgaatact accactcatt tcacttccta atacgacacc
     7561 gccatgtcca tggaccatcg tgcaatttgt gatcgtgata ttttcgcaag cgatcctttc
     7621 gtacgtatct tcagtccctg ccttgattgc aatacagtca tctcccacgt caatatggca
     7681 attactaata cgcacatttt tgcatgattc tggatcaatt ccgtctgtat ttggtgaatc
     7741 cgctgggttc aagatcgtca agttatcaaa cgttgcatta ctgcaaagaa tcgggttgat
     7801 cgtccaactt ggtgactgga tcaatttaat atctttcaca gtaattcgat gacagttatg
     7861 gaaactcatc aatttaggac gaggataagc gagattatct ggttcatttc taaaagtatg
     7921 ccaccatttt tttccatttc catctaatgt accgaaacca gtgacagaga tgttctctac
     7981 attttgagca tagatacagg aagcataaac ttttcgatgg actccttccc aacgtgaatg
     8041 aacaacagga taatcttttg gatcgtcaga gaatttcaga accgctcctg cgcttaaatg
     8101 aagttcgaca ttgcttttta aaaataaagc tcctgttaga aattcacctg ctggaacaac
     8161 tacggtcccg cctccgtcac ttgctgcagc atcaatggct tgttgtattg cttcagtatt
     8221 caattcatca attgatgcgc caaatgtcaa aatatcatac atatttataa tgcctccact
     8281 tcaatcaatg cttgcaagaa tgcaccaacg cctttttgat cattacaaat aattggttca
     8341 ctgatgtagt aagcatatga accatctcgc tgatctgctc cgcctaaccc tgctacttga
     8401 cagtttttat tcaagttcag ccagccttct tttgtttcca gaacaaattc atcaagtaat
     8461 ccttgatgtg atttcttcac ttgtggaagc cattcttctt tgttcagcag tcccaaacgt
     8521 attcctttag ccattgcgta aacaaacata ctactgccgg aagcttccag ataatttcct
     8581 ttccgttttg tttctgttgt tacttgatac catgtcccgc tgttttcttc ttgatatgga
     8641 agtaatgcag ccagtgtctt ttctagaatt tctttcagca aagaacaatc tgtttcttca
     8701 tgaagtaatt cataggtatc tgctgcagcc atcaaatacc agccaatcga tcggctccaa
     8761 aaatgatggg atagacctgt ttttggatca gcccatggtt ggaccttctt ttcatcccat
     8821 gcatgaatca gcaaatttgt ttttggatcg aatagatgtt tataactgat ttcaaactgt
     8881 ctcaaaacat cttggtaatc tttttcttca ctaaaattct ggataaattc agcataaaaa
     8941 ggagaaccca tatataagcc atccaaccaa atttgataag gataaatttc tttatgccaa
     9001 aaagctcctt cggatgtgcg tggatggttt gccaactgct gggaaaggag ttctgctgct
     9061 tttttatatt tttcttcgcc tgtttctttg tacaataaaa agaaaagctt tcctgtattt
     9121 acatggtcga tattgtattc ttcaacgctg tagccacgga tcgttccatc ttcttgcacg
     9181 aaatagtcga tattgtcttt tatgaaatca aaataaattt tttcttgggt tcgttcccaa
     9241 agtaattgga accctttaag tacgacgcca tagtcataag accattttcc tttataccac
     9301 ttttcttcat ataagcgagg tgttctttcc atgatacttt ttgctaattt ctttgaccag
     9361 ttttccatta tttgctctcc tttaaaaatt aaaataggat ttgggacaaa taccaagaag
     9421 tatttgtccc aatcgtctat attactgtgc cgctgctttg tatgcttcct cgtactcttg
     9481 tgtgattcga tcgcctcctt gtgctttcca gtcttctaca gcttttttga atccatcttc
     9541 gtcaagctgt cccatgatgt attgcaccgt agcatcaatg acgatttttt caatttctga
     9601 tcctacttca ttgttcgtcg gtgaatccaa tggcacagaa ggatccatta cagcaaattc
     9661 ttcattttct ttgatcaatt gattagagaa ttctttttca gggtctgtat ctttgaaaga
     9721 gaacgtcact tcactaggac gagagcttga taacggttgc acctcttgtt gccaaagatc
     9781 catatcgata aattcgatag aaccatcatc attcaattca taatgcgttc cttcgatacc
     9841 gccagtcatc aatttataca catcttcttc taaaagatca tcaacaaatt gaagtaattt
     9901 cttcaattgt gcttcgtctt ttacttgaga tttgggaaat gctagcaagc cgccaatacc
     9961 atttccttct gaccaaacat gataaccgcc gccttcacca tctgagatct tgtttacagg
    10021 cactaattcc atatcatctt gaataccttg acccatcgtc ttcagattct tgatatccac
    10081 cattccagtg tagattccag ttcttccttg tgcaaattgt tcttgctgat cagtcttttg
    10141 ggtaactgca aagtcttgtg ccaaatagcc attctcaaat agatctcttg aaaaattcaa
    10201 agctttcata tactcttctg tatcaaattc tggagtgaat ttgccattat cgtctacagc
    10261 ccagccattt ggtgctccaa aataagaagt gaaaagttta aagctggtat aacgaatatc
    10321 actaggcgca cgatcgccaa aaccaactgt gtcgtccaca ccatttccat caggatcgtt
    10381 ttctgtaaat tgtttagcta cttcataaag ctcatccatg gtagttggaa catctagtcc
    10441 tagattatcc aaccagtctt ttcgaatgac taagccagca cgagcgtagt ctttttgtat
    10501 cggtacgccg tacaaggcac cgtcgatact cgcagcttcg attctatctt cagaaatttg
    10561 acttaaattt ttatagtctt ttaaatattc tgatacatcc cagaatacgc ctgattttaa
    10621 cgcattgcgg acagaagagt ttttcaacat cgcaatttga agtgtaacga tatctcctag
    10681 ttcatcagaa gccaatgcag ttgttaatct ttcttctttt gaagcatcag gaatccagtt
    10741 gaatgtcagt tccgtatcgg ttttttcttc gatcaagttc acaatcgtgt ctgtaggcgg
    10801 tgaagcatga tgcaaaatat ttaaccaagt gattttagct tttccatctt ctgtctctga
    10861 agaactgtta cttgaactgc tgccgcatgc ggcaagtgct agaattcctg caaaactaag
    10921 acctaatttt acgagcgttg cttttttcat aaattttctc cttcactgtt ttaataatat
    10981 ttttgttttc ttctgttttt tctgtatatg agttcttcca ttccaaatgg caaaatatag
    11041 aatttgctgt ccacgtgact gcggaaaaga agagactgcc accgataaag gttaaaacac
    11101 ctggcattgt ttggatgatc atcagataaa tccctaccat acttaatatc aacactaatg
    11161 agtaatgaag atgtgagaat ccgaataaaa atgccatttt cattttgaaa aaatatcctt
    11221 tccaatcata atgagccatg attgggaaaa tatagagtgc tgagagcaca tatatgaacg
    11281 cagcaatata caaagcgatc ctaagatacc aattgtgtat gaaagaaaga tccacaagta
    11341 aaacaatccc gatggcgcca tatatccagc tcatcagcaa ggtttcacga tagttttctt
    11401 taaaatatgt gaagtacgtt ttcgtcaaag gaaacgtttc ttttgagcgc agccattttc
    11461 taatcactgc aaccatcgca tacgatgcag gaccgaaagt aaaaactccc aaacctgcga
    11521 tgactaaacc gccccacaga aaattcaaat aaaccaactg taaaatacga tttagccttc
    11581 cgtttatttt atctaatgac tgcagcatat ctttccctct ctttctatct tagtaaacgc
    11641 cgtcttctcc tagctttttg gcaatcttat tagctaaaat taccataaac aatcctacga
    11701 cccctttaaa caatcccatt gctgtactga aacttaactg tccattcttc aatccagctg
    11761 tataaatata ggtgtcgaaa atttctgcaa cgcttcgatt caatgaattc aataacaagt
    11821 acatatgctc gaaccctaag tctaatgtct gaccgatttt tagaatcagc aaggtaatga
    11881 ttactggacg aattgccggc aatgtaacat gccatgtttt gcgtaagcgt cctgctccat
    11941 ccatttctgc agcttcatac aactgtgtat cgactgcagt tatcgctgaa aggtagatga
    12001 tcgtagacca gcccaactct ttccagatca cttgtccgat atatactgtg cgtgtccatt
    12061 caggggatgt tagaaaactg attttttcaa atcccattgt tgcaaggaat tcattcaata
    12121 cccctccgtc aacattcaaa aatacataag taatcgaaac aatgattacc caagacatga
    12181 agtgagggat gtaaatgatc gtttgaatcc cgtttttcaa tcgtttgttc gttacttcat
    12241 ttaatagtaa agacaaaatg attggcatcg gaaaaaacac gaagatattt aatccgaaca
    12301 agatcaacgt atttcttaat aatgtaaaga atgtcggttc tgtaaacaaa cgaatgaaat
    12361 gtttgaaacc gacccaagga cttcccatta ttcctaaaaa tggttgataa tcttgaaaag
    12421 caataacgag tccccccata ggaagatacc taaaaatcaa gaaataaagg actcccggca
    12481 gaatcatcac atataaaaat ttattcgttt tgattcctgc aattattttt tttcgtcttc
    12541 tgagcttttc ttcttttttc gatagcgtca gttgaaatgg ttcttgtttc tttacacttt
    12601 ccataagccc ctccttagtt ttgtgaatgt ttgctgctta tgacttcatg atagataact
    12661 atctgaatta cgtatataat catttttatc gtaagtaaaa acgcttacat agcaatgatt
    12721 tttgtgctat acaaaacgat aaagtgtatt ttttcacaaa taaactacgc atagcaaacc
    12781 ttctattttt ttctatctga cacttagcat aggaaatgat tatatacgta atctttcacc
    12841 gatgataaat tgaacatata gaaacagaaa catttaattt attttgagga ggagcctatc
    12901 tatatgaaaa agaaagtctc tataggagaa agaagtttta cacttttcaa caatacgttt
    12961 ctaattttgc tagctttgat ctgtatcgtt ccttttttaa atatcatcgc tacatctttt
    13021 gcttctacac aagaagtcgt cgcgaaaaag tttatccttt ttcctactac cttttcttta
    13081 gacgcgtatc gctacattct ttctacgccg accattttta gagcacttgc tgtttcgatt
    13141 ggtgttacag gtgtcggaac gatcgtcagt atgtgtgcaa cttcactgat ggcctacggt
    13201 ctttccagaa agtatttgtt tggtcgaggt ttcgtcaact ttcttgttgt attttctatg
    13261 ttgttcagcg gggggatgat tccaacgttc ttagttgttc gttctctagg gctggtcaat
    13321 tcttactggg ccatgatcct gcctgtagct gttaatgcaa tgaatatgat cattatgaga
    13381 aactttttcc aagccttgcc ggatagtttg gaagaatcag caaaaatgga tggttgcaca
    13441 gattttggtg tatttttcaa aattatgctg ccattagcgt taccgtctat tgctactatc
    13501 tcgctttttt atgctgttac ttactggaac acttatatga ctgctatttt gtatatcaac
    13561 gattcatcta aatggccgat acagattctt ttacgccaaa tcgtcattgt atcaagtggg
    13621 atgcaagcag aaagtagtgc cgttgatgtg attccaccag cacaaacagt taaaatggct
    13681 gttatcgtta ttgcaacagt gcctatgctg attgtttatc cttttgttca gaaatatttt
    13741 gtcaaaggag cactcgtagg ttctgtcaaa gggtgattat gtactgaacg caaaatggta
    13801 gttgtgtcag aagtggtcag cttcaagaaa taagaaggca tttacgaaaa ttgcttctta
    13861 aatttttgtg aaatttcggc ttatttttcg aagaatctac tcctggaaca ccgtgtatca
    13921 gaggttgtga aagagacgtt cagccttgag cgatgataaa aactctcaca aacattgcga
    13981 cgagtaaccc acaggagcaa taagaagcga ttttgaaaaa tagcttccca tatttcacaa
    14041 aattatgcct tattgtcgat agcctgtctc ttgaacgcca tttattcgta aaaaagaact
    14101 gtgacaagac ttttgtcaca gttctttttg cgtgcttagc cttgaaattt aactatccat
    14161 aaatacaaat actagggtta ttctaacttc ttttattgct tgaatcagtc cacaagaaaa
    14221 acagcctctt gattaaacgg cgtttatctt gttgattctg cggcaataaa atcagccagc
    14281 agcaaaacat gaagtgctgt tttttgctgt cctcttttat tgcttgaatc agatcacaag
    14341 aaaaacagcc tcttgattca caaatatttt tttcgatatt ctcccggcgt aatcgattcc
    14401 ttctttttaa agaaacgaat aaaattttgc ggatttcgat attgcaatct ttctgcaatc
    14461 tcttttacgg agaggttagt ctctttcaac catttttttg ctacctctaa acgatgattt
    14521 tgaacaaaat caccaaattt ttctcctgtt tcctttttaa atacattact taaatagtta
    14581 gggttataat gcaacctatc agcgatcaca tctaaagaca gatcacgatc atattctgtg
    14641 tagacgatat gcataatatt ctccgacaaa tttttaaatt catgatcagt gattgcttga
    14701 gagcattctg tgattggcag aactaattgt tctaaaacaa gtttttctag ttctttggga
    14761 tgataattgt tcagtgcttt tacataaatt tgtttcatgt tatcaaagtt ttttgaatct
    14821 actcccaata actgtcctaa ttgaacgagt tcattgatca agcggaccga aactacttcc
    14881 cgactcaaag gatttttatt ttgtgcaaac aatgcatcaa tcaattcatg tactaaattc
    14941 ttcacttcct gatttccaga acgaatggct tcaaacaatc gattttgttc ttctatcgga
    15001 tatctaataa gtgttttttc atgagcagca ggaacgattt cctgataaaa aataattgaa
    15061 cttggaccag tatttacacg ataatacagg gcttcttttg cccgatcaac cgattgcttg
    15121 ctttcattca gcgtttcgaa ccggttactg atgcctatac tgatagttaa attcaagtat
    15181 tcttttacac gtttttgaat gagtcggcaa tactccatga cttttttgct tgcttctgca
    15241 tcatctttat ccattcgata aatagtggct tgcatttctt cattcaaaac gatcggcagc
    15301 attcgatttt cctttggcac aatttccgtg atcatgttgt tgatcgctaa aagtagcaaa
    15361 tcacgctccc caccgtgact atcttctaac agatcgattt gaaccaaagc tgtataatag
    15421 cagtcatttt gagaactata accaaattgc tgcaaccgct gatctagttc tcgcctttct
    15481 actcgatttc taaataaact aagtacaaat agcgtttcca gctgtggctt ttgggtaact
    15541 aagtgtgcac ttaacgcttc tttttctcct atgatccgac tgactgattg agcgaccaaa
    15601 tctagttctt tcccactgaa tccttttgat ttaagattca gacgttcctg tatttgagaa
    15661 atcggacggg caaatctgtc cgaaaataag taggaaagaa gaccaatgag tcctaccagt
    15721 gttaaagaca ctgcaactag cccgattctc agattgcgga tcattgcgcc aattgcacta
    15781 gagtcaactt ctaatctgta aatccaatta ttataatctg atttcagtac tatatacgtt
    15841 ttatcttttt ctcttatcac tttcagctga ttgtaatcta gatccacgtc cgttaataca
    15901 gttggttgct ctttattttt tactgtctga gtagtaaaaa ggttgccaac ttcgttttca
    15961 atagaaagta tgtcttctcc actttcacca acaacttgtt ctattgtatg tttctctatc
    16021 gtagcaattc ccaatgcata tttttctttt tcaaacattg gcaaagaaat caccatttcg
    16081 attcctttgt ttttcttttg ccaaaataaa ttatttttgt tatttatgta cttctgttga
    16141 tacttttcaa cttcctcttc ggtcagttga ctcaacgatc cattgattac tccccattta
    16201 ccagccaaac tgactaaatc ataattatta tcatccataa caatcgtttc tataaaattt
    16261 agttctttcc tgatttcaga ataagtggag taatctgtat atgtcagcgg atgcttgaat
    16321 atgtccgaga aatttttggt cgaaccatac gtagaaaacg aatattcaat cgttctaatt
    16381 ttccattcaa cgttagtttt aacttgcctg agcaaattct ttttgtctgt taaataactc
    16441 gattcgatcg attcataggt attctggtaa atgaataaac taaatcctat gacaagtacg
    16501 gctcctaata aaaacatcgg taaaaatatt ttataaaata ctgtactttt cttcattttc
    16561 ttcctctcct ccttttaaaa taaaaggctt tcatttttct gtaaaattga tactcatcta
    16621 attaaatgag cattccccgc tttcaagaat agcataatta aaaataaaaa tccaagtaat
    16681 ttttaacgtg tgcaatcttt tcatatcaaa gatctattga cttatcgtag ttatcgtaat
    16741 atgatgaatg cgttaacaaa aagtgaaaaa gtaaggaaaa tgatggaatg gtaacgatca
    16801 aagatatcgc taaagcagct ggcgtttctc atacgacagt ttctcgtgct ttgaataaca
    16861 gcagtttaat aaaaaatgaa acaaaacaaa aaatacgaca actagctaag gaaatgaatt
    16921 attcgccaaa ctatagtgca aaaggtttag tgaatcaaaa aaaatatcta atcggactat
    16981 ttttctcaag tttgcataaa ggaacttcat caaatttcct agcagattcc attgcaggca
    17041 tccattcagt attagacaca aattacagtt tatctgttga aagtatcgat gttgttgatt
    17101 taacagaaat caacctgcaa cgctacgatg gactgatcgt tatgagtcaa agtgatgatg
    17161 atcagccttt catcgactat ctcaaagaaa aagaatttcc tttcgttgtt gtcaatcgtc
    17221 acatcaatga tccagcggtt atcaacgtca ctgccgatga tgcggccggt gttatgcagg
    17281 cagtagatta cggtattgga tgcggacata cgaagatcgc ttatattggc ggaaagaaaa
    17341 attttcgttc gacaaaagaa cgggaaaaag gggtattgaa aagtctaaaa aaagcgagca
    17401 tccctgtcaa tccagcctat tttttaacag gagattacag cttagaaagc ggcctccaaa
    17461 acatggaata cctattatca ctttctgacc tgcctactct ggttttttgc ggcaacgacg
    17521 acatagcgat tggtgcattg agagctgtac atttagctgg cctccgtgtt ccccagaaca
    17581 tttctctgat cgggttcgat gattctccag tcgtctccta tctcaatccg ccgcttacca
    17641 ccgtacgtaa gcctttgaaa gaaatgtgtc aagaaggaac aaagctttta ctagatttga
    17701 tcgaaggcag gacgatcgaa caatctaaaa ttgagctctc caccttgttg atccttcgtg
    17761 agtcgatcaa ttacttgtaa actacaagta attttatata aaaatgttaa cgtgtgcata
    17821 acacagaaag gaagtttata tgaaaaaact gacaaaagaa ttagtgaaaa aaaacgaagc
    17881 acctgtaaaa gttcttcagt ttggtgaagg caattttatg cgtggattta ttgattggca
    17941 gattcagcag ctgaataacc aacaattgtt taaaggtaat gttgcaatca ttcaaccttt
    18001 agaacaaggt cttggaaata tgatgaacca gcaagataat ttatatacgg tcattttaca
    18061 aggactcagc aatggcgaaa tcgttgatac ttccgagatt atcaccgtag tagaacaaat
    18121 gatcaatcct tatgatgaat gggatgctta tctatctcta gcagaaaatg acgacttggc
    18181 ctatatcgtc tccaatacaa cagaggcagg aatccaattc gtggagactg ataatttaac
    18241 agatactccc ccacagagct tcccgggcaa actgacagcc tttttatacc gacgtttcaa
    18301 attaaacaaa gctggcttta ccatcattcc ttgcgaactg attgacagaa acggcgaacg
    18361 tctaaaagaa atcattttac gctacgcaga aaaatgggat ttagaacctg cttttgttga
    18421 gtggttagaa aaagaaaatg ttttctgttg tagtttagtt gatcgcatcg tcccaggtta
    18481 tcctcgcgaa gatgcaggtg ttttcaatca acggcataac tatgaagatc aattgatggt
    18541 caaagcagaa ccattcatgc tttgggtgat cgaagggcct aaagaattgg agaatcaact
    18601 accattcaaa caggctggct tgaatgtcat catcacagat gatatgactc cttatcgtga
    18661 acgtaaggtg catttgctta atggaccgca tacagcgatg gtccctcttg ctttgttggc
    18721 ggaattggaa actgtagaag aagtaatgaa agatccttta ttctccgcat atgttgatca
    18781 gttattcaat ctagaattga ttcctatgct ttctttacct aaagatgaat tagccattta
    18841 tgctgaccaa atcaaagaac gtttcttgaa tccttttgcc catcacaaat tagaagcgat
    18901 ttcattaaat agtgtttcaa agttcagcac ccgtctgctt ccagtgttca agaagtatat
    18961 cgaagaacaa aatcaggtac caccgttaat cactgtttca ctagctgcgc tacttcttat
    19021 gtatcgaggc gatcaagtaa agccacatga tgatgaaaag acgataagcg aatttactga
    19081 tgcttggtct gataatggaa cagctattcc tcgattattg caaaacgctg cgctttgggg
    19141 agaagatttg tctcaaatac cgaatgtgac agatacagtt caagaaatag ctgcaaagat
    19201 cgatcaaaaa ggtgtccggt cagtattaca agaaatgaaa ggaacgatgg tccatgcttg
    19261 aaaccctgca accaacaatg aaattaaatc gacgagacag tgtggtcgtc gcattaaccc
    19321 ctattccaaa acaaacacaa ctgacgattg atcagcaaac gatcaccact cttgaagata
    19381 tcccgcaagg tcataagatt gcattgggtg atttgaaagc tggagacaac gtgatcaaat
    19441 atggctatcc aatcggtcat gtgacgacag acgtaactgc cggtcaatgg ctgcacactc
    19501 ataatgtaaa aaccaatctt tctggtgaat tggattatcg atatgaaaaa gatgtccatc
    19561 caagtcatta cctttttgag aaccgaacat ttcaaggtta cttgcgaaaa aatggaaaag
    19621 tagggattcg taatgatttg tttttagtac ctaccgttgg gtgtgtgaat ggcattgctg
    19681 aactgatcgt caaacagttc aaggaaaaac atccagatct gggacagttt gaccatatta
    19741 cgattttaaa gcatccttat ggctgttcgc agcttggaaa agatcatcaa aatactcgag
    19801 aaattttagc ggacgcagta aatcatccca atgccggagg agttttagta tttggattag
    19861 gttgtgagaa caatacagtt cctgaattca aaaaaatttt aggtgaatat gatgaagaac
    19921 gagtgaaatt tctagtggct caagatgttt acgatgagat cgaacaaggt gtggctctat
    19981 tagaagagct tttaacagcg gcggaaaatg accagcgcat atcagtcccg ctctctaagt
    20041 tgaatgtcgg tttgaaatgc ggcggttctg atggtctatc gggaataact gctaatcctt
    20101 tacttggtgc attttctgac tacttgattg ctcaaggagg cagcacgatc ttaacagaag
    20161 tgccagaaat gtttggcgca gaacaagtat tgatggcacg agcggaaaat gaagaagttt
    20221 ttgactcaat cgttgacttg atcaatgact tcaaacagta tttcttgtct tacggggaac
    20281 cagtttacga aaacccatct cctggtaata aagcaggtgg gatcactaca ttagaagata
    20341 agtctttagg ctgcacgcaa aaatcaggaa gctctcctgt cgttgatgtg ctacaatatg
    20401 gcgaaaaaat cagaaaacca gggcttagtt tattgcaagc tcccgggaat gatcttgtag
    20461 ctgcctctgc tcttgcttca tccgattgcc agttggtctt gtttaccaca ggaagaggga
    20521 ctccgtttgg ctcatatgtc ccaacggtca aggtatctac gaatacaact ctttttgaac
    20581 gcaaaggtca ttggatggat tttaatgcag gcgtcttatt agaacaacca atggaagtaa
    20641 tcttagaatc gtttgttcaa aaaatcatcg ctgtagctag tggagaagaa acgaaaaatg
    20701 aacaaaatga cgttcgagaa attgccattt ttaaaaacgg agtaacactt tgattttcat
    20761 aaaacagtat aaaaagaaag aaggaagatt tatgttttta tctgatgatt ttttattaaa
    20821 agacgattgg gcgaaaaagc tttaccattc ctacgcaaaa aacatgccca ttattgatta
    20881 tcactgtcac ttagatccaa aagaaattta cgaaaacaac aatttcagca atctgacaga
    20941 agcttggtta ggaggcgatc attacaaatg gcgcttgatg cgagcttgcg gcgtgcctga
    21001 agaaaaaatc acaggaaatg cttctgattt tgaaaaattt ttagcttggt gtcaaaccgt
    21061 accaaaaatt atcggaaatc cgctttacag ttggtcacat cttgaattga agcgtttctt
    21121 cggtattgac ttgttgataa atgaagaaaa tgcagagaaa atttggacac aagcaaatga
    21181 aattttggca gctcctgatt ttcgccgtcg ggaaatcgca aaaaaatcga atgtagaagt
    21241 gatttgtaca acagatgatc ccgcagacga cttgcactat catcagttgc ttgctcatga
    21301 agaaccagat ttgaaggttc taccttcatt ccgtccagat aaagcactca atattgaaaa
    21361 agaaggattt aatgcatggg tcacactact ggaagaagca gctgaccaaa cgatcaccac
    21421 ctatcaagaa ctgatagaag tcttagggca gcggatagaa tacttccatc aacatggatg
    21481 tcgtgtttcc gaccacgctt tagacatctt acgctataaa gaagcggatg aaacagtatt
    21541 agaagagatt tttcaaaaag cacgagctaa caaactatta tcttcagatg aaatcgatgc
    21601 ttatcgtaca gaaacactga tacatttgac ccacttctat cattctcata attggacgat
    21661 gcagctgcac atccatgctt atcgtaactg taatacacaa atgttggaaa aattagggcc
    21721 cgataccggc tatgatggaa tgaatgatct ttctttgact attcctctgc aaaaattatt
    21781 gaaccgtgca gaagaaacgg atcagttgcc aaagacgatc ctttattcat tgaatccgaa
    21841 tgactatcct gcgattttag ctttgatggg ctgtttccaa aaagaacaga atggaaaact
    21901 gcagttaggt tctggatggt ggtacaacga tactcgagct ggtatgcgcg atcaattgac
    21961 acaatttgct gatggtagtg ctttggggaa ttttgtcggc atgctgacag attcaagaag
    22021 tttcctgtcc tacactagac atgaatattt ccgtcgtgtt ctttgtgaat ttttaggcga
    22081 aatggtcatg cgaggagaag cacctgaaga tgaacaactt ttaggaagtc tagttcaagc
    22141 gattagttat acaaatgcta aaaattactt tggctttttt gcctaattga ttaatagtaa
    22201 cagtaacgaa tcaaaaacag gatagtgaaa taacggaatc cgtttatcca cgttttagag
    22261 aagaagacgg accaaatttg ctccgtcttc ttctcttgct gttctgcttc attgcctata
    22321 aacctttatg aaagaagaga taacatgcct actatccata ttgccggtga ttctactgcg
    22381 gcagaaaaag atgaaaacaa gcgtcccgag acaggttggg gagaaaaaat ctctgcttat
    22441 ttttcacccg agatccagat caataaccaa gcaaagaatg gacgcagcac caaatcattt
    22501 atccaggaag gaaggttagc ccgtctagct tcacagtttc agccgaatga tttcttattt
    22561 gtacaatttg gacataacga ccagaaaatc caagaagaaa gaggtacgct tcccttcggt
    22621 agctatttaa aaaatctgca gatatttttt gaatgcgcaa aaaaagcgaa cgtccaaatg
    22681 gtcttactga catctgtgac gcgacgagat tacttagaaa acggaacatt gaatccagat
    22741 atacttggag actatccaaa agcgatgcgt gcatttgctg agaaacacca tattcctctt
    22801 ttagatgtgt tctcaagatc acaagaattg tcccagacct tctcaaaaga agagacaaaa
    22861 aaattctacc tgcatcttat gcctgatact tgtaaaaatt acccagaagg actaaaagac
    22921 aatacccact ttagtccaga aggcgctgat aaagttgccc gaataattgt tgaattgata
    22981 aaagaatccc gtctgcctct tgctaattat ttacaaaaag gagtttgaga tgtttccatt
    23041 aaatttaggc atccgtgccc acgatttaga tgttcgaaat cgtgaagaaa tcgttcaaaa
    23101 agtatcacag cttcacttgt cccatatcca atttgcacca caaaaagcat ttcctgaaca
    23161 acttaaatta actgacatga cacttgggag tgctgcctat tttggtgaat acttccaaaa
    23221 aaacaatatc gaactttcca ttttaggatg ctatatcaac ctatccagca aaaatcaaga
    23281 agttcgtgca caagcattgg caactttcaa acagcaaatt gctctgtgct ctgcctatca
    23341 agccaagatg attgctacgg aaaccggtag tgtctctgtc ggttacacaa aagaaaattt
    23401 tactgaagaa gcttatcaga tcgccagaaa ttctatcatt gaattagtcg ctcatgcaga
    23461 aaattttggc gtaactgttg ctgttgaagc agggatcaat catccaatct ataactatca
    23521 gttagctaaa cgtttaatcg atgaagtagc ttcaccaaat ttaaaaatca ttttagattg
    23581 tgcgaatttg atgcataaag aaaatcatgc tgatcaggaa aaagtgatcc gaggtgcctt
    23641 ggacaattta catggttata ttacagcgct tcatctcaaa gactatatca tggaaaatgg
    23701 aaagatccgc atggtacctg ttggcaaggg ctgtttagat ttccgcccgg tactgcatta
    23761 tatcaaatat gagcgtcccc taatgtacgc cactcttgaa gctactcctg agatttatgt
    23821 tcctgaagca atagaacact tgcagtttat ttaccaagat tgttaaggag aagattatgg
    23881 aggaacgaat acagaaaagt tatcaaaaat atcatgcaaa tgcttttcag acattgctgc
    23941 atctttatca gggaaattat tggcgttttg ccatgtcagg actctttttt attatcaaac
    24001 atcttccatc ttggttaatg ccgatcgcta ttgccaatgt aatcaatgca gcttcatcga
    24061 aagattcttc tcagttgcag gccatttatt tcaacgcttt tttgatggct tgtctgattg
    24121 gtcaaaatat attgacgaat tattttcatg taaaatacca tagcacttcg atccgtcaag
    24181 tagaagctgg actaagagcg acccttatca agaaaatcca agaactatct attccttatc
    24241 aaaaagaatt gcaatctgga cgaatccagt caaagatcat ccgtgatgta gaagctatcc
    24301 aaacgttgtc ctctcaagta tttgtcagcg gattaaatat cgctgtcaat ttattcgtag
    24361 ctttaggtat cacgctttat aaaagtcctg ttgttttcat gtttttctta ttgactgttc
    24421 ctctcgccgc attcgttact ctctttttcc gtaaaaggat cagacagtcc aactcagact
    24481 tcaggcaaga aatggaagag acttctgcaa aagtgatcga agtgatcgaa ttagcaccga
    24541 ttgctcgagc tcatgggtta gaaaaacacg aaacaactcg cttaaataag cattttactc
    24601 aaatctatga aaaaggattc cacttagata tcctgcaatc tgttttcgga tcagtcagct
    24661 gggctagttt tcagatattt cagctgattt gtttgatttt cactgggata ttagctttac
    24721 aagggcgcat ccaacccggc gatgtcgtga tgtatcagac gtatttcatg acgatcgtca
    24781 attccgtttc tggaatgatc acgctgattc ctacgatttc aaaaggcatg gaatctatca
    24841 cttctattgg cgaactgttg attgctggcg atgtagaaac ttatcacggt aagaaaaaaa
    24901 tccaacattt ggatggaaat tttcgttttg aggatgtttc ttttctttat ccggactcac
    24961 tcaaaccgat tatcgatcat ttttctctag aggtgaaagc tggagaaaca attgcttttg
    25021 ttgggccatc tggctctgga aaatctacac tccttaactt attgattgga tttatccagc
    25081 cgacttcagg aaaaattttt ctagatgatc agcctttgga tgaactggat atgcgaagtg
    25141 tgcgaaatta tttagccgtt gttcctcaaa ccactttgtt gttctccgct tcgatcaaag
    25201 agaacatcac ttatggcttg aaaaacgtat caaaagaacg tttagatgaa gtcatcgaag
    25261 ctgcgcaact ttcttcactg attgaccagc ttcctgatgg tctggatacc ttagtaggcg
    25321 aacatggcaa caagctgtct ggtggccaaa agcaacgtat ctccattgca cgagcgttga
    25381 tacgtcagcc aaaaatcatt ttgctagatg aagcaacctc agctttagat aaccagtcag
    25441 agaaaaaaat ccagcaagcc ctgaattacc tgacagaaac acccactact tttattgtgg
    25501 ctcatcggct ttcaacgatc aaagaggcag ataaaatcgt tgtgatcgat gaaggaaaaa
    25561 tcgttgaaat aggtacttac gaaaaactgt tagctcaaaa aggctatttc taccgcttga
    25621 aaaatgaatg acaaaaaata ggagctatga caagactttt gtcgtagctc ctatttctga
    25681 ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatagtcat caaacatgag
    25741 aagctgtttt ttgaaattgt tccttgtaac tcagagttaa acggtaggct cacgacctct
    25801 tggtaaatgg tgttcaagag acagcctatc ggcaataaga agcaattttg tcatcgctca
    25861 aggccgaacg tctctttcac accctctgtt gtttcataaa atagttttat tcgctgtaaa
    25921 ggtcgcgaac tggtgtcatg atgatacggt tcaagtcatt gatgattgta ctgaagcctt
    25981 gctctttttg catcaagtca ttgattactt cttctgattg gattttcaca gccatttctt
    26041 gtgctttcat tgcatcttcg tcgctgaact cttgtccact catttgtttt tcttgtaatt
    26101 cgatttgcat cgcttggaag tctttgaaca gtttgtgtgc ttcttcgttt gcttgtactt
    26161 tctcgtacgc ttctttcaac gctttgaatt ctggtaattc acggatctca cgttcgattt
    26221 gattcgcgct atcgtagatg tttgacatgt tatgccctct tttcttttag tagttactct
    26281 ctattgtaac atggggttat tcgttttcaa aggggctact ctccttgata tttccgccag
    26341 aggtctttag ccgcttcacc aattccctga acgccttctt tcaccatatc gccgaagttt
    26401 ttcagccctt cttttgcttg ttcaccatat tgattcaagt tatcttgcca atttccagac
    26461 tctccattag aatttcctgt gtttccaact tgatctgcag ctaccacttg tcctcctgtg
    26521 gcataggcat ctgctacact aaatcctgtt tttgaagtat acggaaggat gccttctgcc
    26581 gctgatttga agactttccc gacaacattt ccacttgtcc cttccaaata gtgcagcttg
    26641 ctggattctt cgtaacccat ccatgtcgaa atcactacgt ctggcgtgta accgatcatc
    26701 cattggtcat ttgcttttgt ggcatcgaaa ttagtttctg tggttcctgt tttccctgct
    26761 atcgtataac ctgcaggatt cgcagcagcc gctgttccat ttgaaaatgt accaagaagc
    26821 atgctggtca tttcatcggc tgtttccttt gatatgacct gttttggttt tttgtttgtg
    26881 ttatcgacga tcaccgctcc agtcgaatca acgattttcg tgataagatg cggcgtatac
    26941 atctgaccat cattggcaaa tgcactgtaa gcacccgcca tgatcaatgg agatactcct
    27001 ttttccagtc caccaagggc taagccataa tacttatcag attttgctaa aggcaggccg
    27061 aattcttctg ctttatcata tcctttttgt aaaccgatct catgcaaaag ccatacagca
    27121 ggcaaattca aactttgtgc aacagcttga tacatcggta cttctccttg gtatgtcccg
    27181 tcaaaattat gcgcatcgta ataactttgc ggttcgtctt tcaagatact ggaaggcttg
    27241 tatccagctt ccagtgctgg tgtatatacg cttaatggtt tgatcgttga tcctggtgaa
    27301 cgacgcatct gtgtagcaaa gctgaatcct ctaaagacat gatctcctct tcgtcctact
    27361 agtgcttgta caccacctgt attcggatct aaagctactg aagcagattg gaccatggca
    27421 ccatctgaag catttgctgg gaataatgca ctgttttgat aagtagcttc taattgcttt
    27481 tgataatcct gatctaaagc tgtatagatt ttatagcctt tattcatgac atcttcttca
    27541 tcaagaccat actcgttaac tgcttcatcg atcactgcat caaaataata aggataacga
    27601 taaccagaat ctgaattatc gtatgtgtca ttaagatacg cgctgatatc tgtctggctt
    27661 tctgcttgtt cttgttcttt tgtgattttt ccgttttctt ccattacact tagtactgtg
    27721 tttttccgat cattcgcatt ttttgggtga tcgatcggat tataaatacc aggacctttc
    27781 agcattcctg ccaaagtagc agcctcagac agcgtaacat tcgttgcatc tactccaaaa
    27841 tactttctgg aagcgtcttg gacaccccat accccgttgc caaaataggc attgttaaga
    27901 tacatggtca agatttcttc tttgctgtac ttcttttcga tctcgattgc caaaaataat
    27961 tccttcgctt ttcgatcaaa cgtttggtct aatgtcagat acgcattttt ggcaagctgc
    28021 tgagtgatcg tacttcctcc gccccctgat gttccgccag acaagaccat tcggactgct
    28081 gcacgggcaa tccctttgat atcaaacccg tgatgctcgt aaaaattcct atcttctgtc
    28141 gagattactg catcttggat atagggagaa atcgcgttta attctacata tgttcctttt
    28201 tgggcgtaca gtgtgccagc ttcttcgttt ttcttatcat aaatgactgt cgattggctt
    28261 aatcctgatt ttaacgtttc gacatttgct tgttttgcta aaataaagag gtagatactt
    28321 gtcactaaga cacagatcaa accaatcagc aaaatcaatt tgttcacatg atattttttc
    28381 cagatgcgct ttcgccatcg atggaattgt ccgagatatg gtctgatcca acgccaaaaa
    28441 ctgatcaaag cagcttttat cttttctaaa atgctttgaa aatccatttg cttcttctcc
    28501 tgtctatttg attcaatcgt cttattttac catagtgaaa gagtgttttc ttcctgctta
    28561 agttgtagtt caatatgttt ttttacgtta gtgcaagtag agtaacataa gaatgaacag
    28621 aaaaatgtaa gttttttctt aaatatgttt taaaaatggt acaattagtc tgcaattgca
    28681 ctgaaaggag tacacttgtg aaaatcacga tcactttacc tatgaaccat cctgaaacaa
    28741 caattcgcga cttgcttgaa aaggaatggt tagttcctag aaaggttcga cactttttgc
    28801 gtatacgtaa aaacgtttgg atcaatcaac aacctgcttt gttccatcat gaagtaaagg
    28861 gcaatgacca gatcacattg ttattcgaag aaacagatta taattatcaa gaagttcaat
    28921 taggagatgc gaagcacatc cgtgttttat atgaagatga gcatttgatt atagtggata
    28981 agcctgcagg aatgaagact catccaaatg aaccgacaga aaacagtacc ttactcaatc
    29041 atttagctgc ttatctcgac actaaacagc aacgacctta cgttgtccac cgcttggaca
    29101 aagaaacaag cggtgccgtt ttatttgcaa aaaatccatt tgtcttgccg atacttggaa
    29161 gaatgttgga acaaaaaatg atctatcgga gatatcaagc catcgtatgg ggagaaattg
    29221 ccaaagatat gaccatcacc aataaaattg gacgagatcg ccatgatcgg agaaaaaggg
    29281 tcgtagatga taaaaatggt cagagcgcct tgacacatgt cactgtcaac aaagtacttt
    29341 ctgaacaaac acaagtctat tgtgttttgg aaaccggacg tacacaccag atcagagttc
    29401 atttagctca taagggacat ccgatcgttg gagaccctct ctaccaaaat agaacagctg
    29461 aacgactgat gctccatgca ttagaactgc acatgatcca tccatttact caagaaaaaa
    29521 tagtcggtaa agctttacct ggattatggg aatagaaata aaaaagaaca gagtcttatc
    29581 agcagataat gataagactc tgttcttttt gtgtaatttg tctgcttact tttgatgttc
    29641 gtgttctgct aacagcaaac taccgataat cccactttca ttcggcagtc cccaacgaac
    29701 gatatattca cttacttgag gtgtttccat ataaccaccc atcaattcaa caaattgctg
    29761 acggattttt tgaaggagat gatcctgatt cattacccca ccgcctaaaa taatcttttc
    29821 aggtgctaac gtcaacgtat agttgatcag tgcttgagca atataataag cctgaatagc
    29881 ccaaacagga tgatcttgcg gcagatcttg ccctttgatc cctgtgcgtg cctcaataga
    29941 cggtcctgct gctaatcctt ctaaacaatc tttatgataa ggacaagtac cttcataatg
    30001 gtcatctgga tggcgtttca cccaaatatg tcccatttca ggatgtgcga tccctgaaaa
    30061 aatttcttca ttgatcacga cacctgcacc gattcctgtt cccacggtaa ggtaaataca
    30121 actatttttt ccttgtgctg ctccttgctt caattcccca tatgcagcag cattgacatc
    30181 cgttgtccaa gcagcaggaa tattgtagcg ttccttgatt gtgccaagaa aatcgaattg
    30241 tgcccaccct tttttgggag ttgccaaaac gtatccgtaa ttatcttttg ttttgtcgat
    30301 accaatcggg ccgaatgagc caatcccgat tcccttcaat tcataacgat caaaaaattc
    30361 aaatacctga tccaatgttt cttctggtgt tgtggtaggg atacttaatt tttctaaaat
    30421 ttctccacca tctgaaaccg cacacacaaa ttttgttccg cctgcttcta ttccgccata
    30481 cattcgtttt cctcctcgaa tccgattgtc cgaatctcat aaggttggat tattgggaca
    30541 accgcttgct tcgttggttc ttcaagcaga ttcaatatga cagtttttcc attgtcttta
    30601 gttatttcca atttttctaa atgactagac atattgaaac ctcttactac tactttatca
    30661 ctaaacttgc tgcgtttcaa agctgtaatt gcaaacgttt cactttttat cgttaaatat
    30721 tgttgttttg aaatcaatgt tccttcatga tgtttgattt gttgagtgga aaaaggaatc
    30781 tgtgctgcgt atgcatgcaa ataagtagag aatttttctt ctggtccatg tagttcaatg
    30841 ctgtaattga accgatgctc acctagacac tgtgcttctg gtgttggaaa atatccccaa
    30901 tcgcctagtt ctcccacact gcgaagtagc gtcactgcga tttgtccgtc ttctgttact
    30961 tcatattcat tcaaaccaaa gtttccgact gttacaccgt actcttcatc atgaatattg
    31021 acaaatgctt gctgatgttg cggattcgtg gggttttccc aacttttaga cacatggttt
    31081 gggcgcttca ccacttcaaa aatactatct gcttcgtgat gttccacatg taactttgta
    31141 ggaaacaaga cacgtaagcg atgatctttc atctgattgt cgagagtcgt ttcaaagtcg
    31201 atttttttac tatcttttct gattgtcatg attgttttta tttgaaggat tcgtttttct
    31261 ttggatcgtt ctgcttttcg ataacgaaat tccacaacca tctgctgttc tttttctaaa
    31321 cgctcgtctg cagaaacagg gatttccatt tcctgtagaa tacttatttt tgctatttcc
    31381 ggtgtatcta ctaatacttt tacttcagag ttttctttgt tactggatag gattggctga
    31441 tcacagaaag gttgtttaaa gatatactca tttcctatat cgccgatatc ttcaaaaata
    31501 tgcagattat tcaactttcg tttgtttttg cgatcttcca ttgtaatggt accatttttt
    31561 tcaatcgtca aatgtagagg gccgttttcg atgattcgtc cactctgatg aatcattgtt
    31621 tccttttctt ctgctttcgc ttccccttct acaagcgcga atgtttccca agaaaatgct
    31681 ggcatgtttt ctaatggtaa agtcactttg atcattcttg ccatatatgg cacacggaaa
    31741 cggtctttcg gcaaatcata accaaattgg acagtttctt cagataaaat agctggtata
    31801 gtggccccac tttttgtttc tacatgatac ttacgtttag gcaaattttc taactcttga
    31861 tatagttgtt caggtattcc ttcagcgaat ctttttcttt ctaattcgat ggttatttct
    31921 gcttcaccag ttttatccat tcctgcagta tttacaatga caaatggaaa gctgtctttt
    31981 gggaatccgg tcgtatcgat tgctgcggtc agctgttcta aggaatcttg tgcaagatac
    32041 tttccaactt catctgcttt ttcaaaacga ggtatcattt cccgatggac agaatcgact
    32101 gaacaaccgc aaatactgtc gtgaggatga ttttgcatta atgtcttcca agcataatct
    32161 aattgatcat ggggatagtt tccactaact tcataagcca tcgtcgcaag tggttctgta
    32221 atattttcta gctgtcgctg gaccttggta ttccattgtt ttaagtaaac ccgagcagat
    32281 gctgtattgg ctaaagtata ccagccatcc gtttcttgac ttgtcaattc tccttctact
    32341 gagcctaagt cttctggcac atctttttga actgcttcca gatagtcagt aaaattagaa
    32401 tgaataaact cgtaatctgg aaacagttca tttgcgagat ggattgcttt tgatatatct
    32461 ttttgtaccg gttgatgatc aacaccattc atcatcaata agtggttcgt agaggcatat
    32521 tgttctgcat ccgctaattt ttgcttccaa aaggcaagag ctgcttcttt ttctactgga
    32581 atctcattcc cgttgctata ccaattggca aatagcagac caaagattgc tgtttgatca
    32641 ggtcctttcc accacatctc tgagtattgg gaagaatagt tttctgcttc caaaacttga
    32701 ttatcaaagc caattggttt tacaccacgg ccaaacgctg ctgctgaaat accagcttgt
    32761 ttcattaatt gaggcgtctg tcccatattt ccaaatgtat cgggaaaata gcctaacatg
    32821 acaggggttc cccatttacg gctttcttcc atgccaatca gcatatttct cacgttcgat
    32881 tccgagctga taagaaaatc atcttgcaag atataaaatg gtccaattct caattttcct
    32941 tcattgattg cttgttggat tgcctgtctt ttttctggcc ttacttgaag atagtcatct
    33001 aaaataatcg tttgcccatc caaatgaaaa ctgttgaagg acggatcaac ttcaaatagc
    33061 tctagtagat cgtctatcaa ttcaatcagt cgcatgtgat gctgttcgta agccatgtac
    33121 cattcacgat cccagtggct atgagaaatg atatatactt tctttttcat ttacgatacc
    33181 tcctactttt ctactctaat atcaaagtaa tccatgacta attcgcaaaa catcatattt
    33241 gcccaagaaa accattctct cgtataattt tcaggattgt tcacatcgaa tccttcgtgc
    33301 ataaggtgtg ttcctgcgtc agttgctaca agtaaattca atatccgctc tttttcagat
    33361 ttgtctgacg ttgtcattcc ttccatagct aatgcaatcg gccaaacgta tttttctggc
    33421 gtatgggagg atccaattcc ttttgcgtac tttccttcat agaaatatgg attttcttta
    33481 ctcaacaatg ttcttcttgt ttgcagataa tcttcctctt gttcagaaca atatcccaag
    33541 taaggtgcgg cgatcagatt gggcacatta ctgtcatcca tgactgtggc gtttcccatt
    33601 ccatctactt catatgcata aatcgtttgc cctttttgat taaccgttcg tccgtgattc
    33661 tgaatcccct cttggatctc tttcttcaat ttctgtgttt gctgtacaat ttctttatca
    33721 tcgagtatcg tttgaaaaat ttcttccaca taacctaaca cgaccaccgc aaacatattg
    33781 gatggtacca gatatccata acggcaagca tcatcgctag gacggaaacc agaccatgtc
    33841 attcctgtaa aagctgtttt tgcacctcgc ccgtcattga tcagcgtatc ttcttttctt
    33901 gtcgtgtctc gtacaaatgt ataaggagag tgttcatgat cttgctctgt tttgaacacg
    33961 tccaagattt ttttgatacc ttcaataaaa gattgattga attgatcagt ttttcctgta
    34021 tttttgtaaa gaagataggc caactgtatt gggtagcata aagaatcaat ttcatatttt
    34081 ctttcccaga tccaatcgtt catttttgta tgatctgttt ggtggccagc accgttagct
    34141 tcttcgttga aggcattcgc atatggatcg atattgatat aaaaaaattg ctttttgact
    34201 aacccgctga tcatgtcagc taattcctca tcttcttttg caatcactaa ataaggacga
    34261 acttgtgcag tagagtctct taaccacatc gctggaatgt ctcccgtcaa taaaaaggtg
    34321 gttccgtcat tttgcttttt gactgttgtc aacagagtat tggcaaatgc tgctgtaaaa
    34381 ttctttgccc agtccgcatg gatatcccca catttttgtg tgatatcttc cataaatttc
    34441 tgcaccgatt ttggtatatc tttataaacc actaataaaa ccttctttct atttgatata
    34501 tctttaaaga ttatacttca tacagccaat tatgtctacg ctttcaaaaa atatcttttt
    34561 atttactttt tcatccatta tggtattatt tgaatatgat atatcattaa gaggtgttta
    34621 tatgaatcaa ccattgtacc agacaatatt taatgatttg caaacggcta ttttaactgg
    34681 tgaattacct gtagacagcc aactaccaac tgaaaaagaa ctttcacgta cctataaagt
    34741 aagcagaatc acttccaaac gtgcattgac tgaattagag caattaggac ttatttatcg
    34801 cgttcgcggg aaaggcagtt tcgttaaatc cgcttctccg gaacagattc ttgcttctcc
    34861 tacaaaaaca cgacggattc tgtttcttct tccatatgtc gctgatttgt ctgttggtga
    34921 ttttagcaag ggattgaacc ctgtgatgca agataaacag attgatgtca tgatgacgac
    34981 gcttgacttt ctacaaaata aaacagcaaa agagctaatc aatgaatttg acggattgat
    35041 ttattatgct cttgatacag aacaacatct tgacttactt tttgagcttt cattgaaaga
    35101 attccctgtc gtcatattag ataaaaaaat ctatgagtta ccttttccta ccgttctttc
    35161 cgataatttc caaggcggtg cgcttgctgc ctcttatttg atcgaacagg gacattcaaa
    35221 aatcgcttat ttgtttggta cgcagactca tccacaatcc gttcgacagc gttaccttgg
    35281 ttaccttgaa gcattggaca aagctggttt gactttccac acttcactgg atgacaaaag
    35341 cgcgacatta gatgaattgc tttcttatat cactaagtat gaagtaactg cttttgtttg
    35401 tgaaaacgat gttgtcgcta tccagaccat gaatctcttg agacagcatg gttatcatat
    35461 ccctgaagat ttttcaatta tcggatttga tgatatccaa gcagcagctc ttgttgaccc
    35521 gccactgaca accatcgcac aagattttga acagttaggt aagcttgcgg gagatgcttt
    35581 ggtcacttgg ttagagacaa aagatcgacc atccgatagt aaatttccag tttcattcat
    35641 caaaagacaa tcaacaaagg agaaataaaa tgaatatcgg tcaaatagat actagacatg
    35701 caacagcgaa tcagccaaat ttctctaatg ggaactgcca gccttatact ggcgttcctt
    35761 ttgggatgaa ttattttgca ccgcaaacta cggatcaaaa cggcagctgg tggtttcatc
    35821 ctgatgaccg cgtttttcaa ggctaccgtc ttacccatca accaagtcct tggatgggtg
    35881 atttcagtca tatgctgttt acgccgatca atggaaaact acaggagaac acccttttcc
    35941 atgctcaaag ttcttaccgc ccagaagaaa gtatattttg cccgactcat ctgtcgatct
    36001 gtcaattacg ttatggcatc tgttctacat tgatacctag catgtatggc ggaatcttat
    36061 ctattgatta cagcaaaaat gacagcggtt tacttttatc ctttccggga cgccatcagc
    36121 tctttgttat agatccttac acagtggaag gaaaaatcac acattttgct ggttcagaag
    36181 atcctgattt ttctttttat tttatattga gatttgaata tcctttaaca attgatgagt
    36241 tagctgtttc caacaaaaat agcgactcga tcccgttgta ttttgaacag acaaaaaaac
    36301 aaactatccg atttgggaca tcttttatca gctcggaaca agcagcttat aacctggaac
    36361 tagaacaaaa ccaaagcaaa gagacttacc ttgctcacag tcaagaacaa tggcagtcct
    36421 attttgatcg tatacagctc acacaccatg atcaacaaca attgcggacg ttttatcata
    36481 atttatatcg ggtgttttta tttccacaaa cattttatga aattgatcct agtgggcaaa
    36541 aaatccacta tgatacgact agtcgatcta taaaacctgg tgttctctat acaaataatg
    36601 ggttttggga tacgtataaa acggtttatc ctctttattc tttgattgct gtagaaaaat
    36661 acgaagaaat gctggaaggt tttttgaaca gctatagaga aagtggctat ttgccaaaat
    36721 ggctgtctcc tgatgaacgt ggacttatgc ctgggacatt gatcgatgcg gtcattgcag
    36781 atgctgctgt aaaagatatc cgacgagact tgatgcctga atttctaacg gcaatggaaa
    36841 aaggtgctac ggttcaaagc aaacatccaa attatggacg ccaaggaaca gaagattact
    36901 tgacatatgg ctatgttcct aatcactacc acgaatcagt caatcacaca ttagactatg
    36961 cttatagcga tttttgtatt agccaagttg ccaaggtact agaagaagag gaaatgacga
    37021 agaaatatca gcagcaatca ttgaactatc aaaatatctt tgactatgag tcaggattta
    37081 tgcgttcaaa agataaagat ggcaagttca gagaaccatt ttcagctaat cgttggggac
    37141 aagattatgc agaaggaagt gcttggcaaa gcagctttgc tgtttatcac gatttttcag
    37201 gactgatcgg aaaatatggt ggcagtgaga gatataaaga aaaactcatt gagctgtgca
    37261 atcagccccc aacgtttgac ccaggaggat atggttttga gatccacgaa atgagtgaga
    37321 tggccgcgat agaatttgga caattagcta tttcaaacca gccaagtttt cactatcctt
    37381 acttgttcag ttatatagga caacctgaaa tggcacagcc actgcttaaa cagttgatga
    37441 ctcagacctt cgacgattct ccaatgggtt accctggaga tgaggacaat ggaagtatgg
    37501 ccgcttggta tatcttcaat agtctgggat tttatcctgt cacgcctgga accggtgaat
    37561 atgttatcgg tatgccatta attgacaagg ctgttttgct tttgtcaaat ggaaaggaat
    37621 tgacgattga agcaacgcct aatcggccgc agcaacaatt tatccataaa atcaaacgga
    37681 tggacaaaga atatacgaaa ttatatttta cccatcaaga tttgctgaaa ggcggcacga
    37741 tctcttacca attagggatc atccctcacc ctaagaaata tcaagcaaaa gatttgcctt
    37801 tttccttatc aaactataat taacaagagg ttgtgaaaaa gctgtcctgc atcaagtaat
    37861 aagaacagcc aagcaaaaat agatcctcat atttttcgtt tggttggtct tattaccgca
    37921 gatgcaagct tttgaacacc gtttattgag aggttgtgaa atcagcgttc tgacctgagt
    37981 attagagaga caaatggcaa aatagttcct catattttgc cattttttga gctaataccg
    38041 agggtcagct gattgaacac cgtttagtgg agaggtcgtg aaaaagctgt ttagtttcga
    38101 gtattaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tggcttaata
    38161 ccgagaaact ggcttttgaa ctccgtttat cgagaggtca tgaaaaagct gccttgcctc
    38221 aagtaataag aacagcccaa aattagattt ctagacaatt ttttataagt tatttcatta
    38281 cattggcttt ttcttttatg attctccact gttttctcgg tactctttag gggatctgcc
    38341 gacgattttc ttgaataatt tagagaaata cgtggtgttg ttgtagccaa tctcacttgc
    38401 tatctcattg atgttttgat tactgtgcaa aagcagttgt ttcgcttttt ccatacgtaa
    38461 atggttcaaa taagctgaaa aacttttttt cgtttctttt ttgaacagct gtcctagata
    38521 catgacattt aaatgcaggt tttctgacac ttctttgagt gtaagtggtt cttgataatg
    38581 ctggtgcagg attacgatta ctttttccac attgtcactg tactttttct gtctctgatg
    38641 ctttttgatg aatgacagaa gtagctgctg aagttcttgc accgtttttg cctgatggat
    38701 tttttcgatt ccttgtaaat attcctcatc ttccagatca atcaattccc gttgaatctc
    38761 cataaacagt aaaaaactgg aatatcgtat atcttccggc atcatggctg ctaactgaaa
    38821 ctgttcaaaa aaatcagtga tcattttttg agcgacttcc aattgtttat tttgcagcac
    38881 gcgtctgaaa cttgaaaaat cgatggtttg atcttttgtt tctgcttgta ctgcgtaaaa
    38941 aacatgctcg tctttatctc cataaaattg atgcaactgt agattgtctt tcgcttgttg
    39001 gaatgtttca gggattttgt ccaaagaatc tgtctcttcc ccgatactga tcagccattc
    39061 ctgatcagga aagttttctt gaacaaaaca acagaagcca tcaacttcat ctgcctccaa
    39121 caacaacgtg atcaatacac ggtctccgta atttcgttga tagtaaaaga tttgttgccg
    39181 ttcttgcaac catctattga taagagtatc atgagttctt gccagttgaa tcaatactat
    39241 cctatgctgc ttctcactta tttgtcctat tatttcttct tcacttattt catccaattc
    39301 ttcattcaac cattgactga ataacagttc ttgatagatt tcttgttgat ttttttgttc
    39361 attttgctga tctaaccgtg ttttgatttt cttgaggctt tcaaccaatt cgaacttatt
    39421 cacgggtttc atcaaataat tcacggcgcc taattgcata cctcctttca aataatcgaa
    39481 ttcttggtat cctgataaaa tcataaattc aaaattatga cgttcttttt gagccgcttc
    39541 aataaattcc agtccattta tttctggcat tgtcacatcc gttactacta aatcaatctg
    39601 tcgctgttca agtacagcta gtccttcttt ggcattctct gctgttgcta ctatttgaaa
    39661 gcctaaactt tgccaatcaa ttatcttttt taacccattc aaaatcatgt attcatcatc
    39721 taccaataac acttgataca cgctcatccc tccttgaatg caatagaaac ggtcagtcca
    39781 cctgtctcat tattctcaag cgacatataa taactgctgc cgaagctggc acgtaaacgt
    39841 tcattcacgt tttgcagacc gatagaaccg tgaagttcca ctttaggatg agacagtttt
    39901 tcttcaatct gttttagccg tttttcagtc atcccttttc cattatcttt gatgatgatc
    39961 cttacccgtt tgccttcctg caagactttg acacttagcg cattatcgaa tcgagtaaaa
    40021 tcaataccgt gtttaaaata attttcaact aatggctgga tcacaaattt aggtacttcg
    40081 atcttttcca aatcaggatc gatcataaaa tgataggcta ctcgattagg ataacgcatt
    40141 tgatacaaat agacatattt ttcgcaaaag tccagttctt cttttaaagt gatcgttttc
    40201 tcttgattcg tattgtttcg aagcaaagcg gaaaacgcat acacaacatc tgctaattct
    40261 tcacttcctt cgctgattgc atacattcgg atatattcta atgtattata taaaaagtgt
    40321 ggattgatct gtgcttgaag cgcccgcata tgtgcgtctt gctgtttgat ttctaatttg
    40381 tagatgtctt ctacaaactg attgatgcta tccaacattt cgttgatacc gattgaaagt
    40441 tccttcagtt catattccgt gtttgttatg tcgatccgcg tctctaaatt ccctgctgct
    40501 gtttgatcca tggcctgcat gacgatagat acttgctgtg aatatcgttt gaatgtttta
    40561 taaagaaaga taagcagtat attgtctaaa ataatgatac caatgatcag cggggcaaga
    40621 ttttttcccg tttcgatttt tacacttttt ttatctagta cagctaatat acgataatca
    40681 tcagacagtt cttggtgttg aacaaaattg tttttttcca gatcgtcaat cggcaattgt
    40741 gaatttttct tcagactctc tgtgatgatc atttcttgtc tttcaaacgt ttctttagct
    40801 attttattat catggaatgt gtatagtcga tttgtcgttc cagaaatcat gtataaagag
    40861 agtccattga atgacgttag attttctaaa atcatatcta gttctttctt ttgaaaacca
    40921 ataaaaatac ttcctgactc agctcctcct tgtgtgatag gcaacttgat ataaaaggcg
    40981 tctagtaatt ttggtgtccc cttttttatt tccccacctt tgttttctct agaagattca
    41041 taatactccg gtagttgatt caacacgata tatatcgcac ttagttcttc ataatttgcg
    41101 tagagatctt tgatttgatt tgggaaagaa actaattcat cggtactgtt ttgctgttcg
    41161 tatacataca tcagatactg atctatgggt tttgttaagt aggtcgtcac attttcgatc
    41221 ttttcggaat tccctacaag ctggtttttg atgatcctac tcaaggttgt tttatcattc
    41281 agcattctgc ttgtgctttg aacggcatca cttgtcatgt tttctgcctg tttttgattt
    41341 tgttcccaag tatggatact aaaaataacg gtagcagtca tcgttacacc gatcatgata
    41401 accgcgtatt tccgcaacag acgatttagt aaggttcctt ttgaaaatag aatccgaaac
    41461 ttattcataa ctttccaact gtttatctaa ttcttcacgc caatgtgttg tcatcgtacc
    41521 attccaaacc gtcaaaagac caatgacacc aaacaggatc aatcctttga attgccaagt
    41581 cacgcctaaa attataccgc taccaataat tatttttaga aaagacgaaa agctggaaaa
    41641 aaccgaaata aaacttagct ttagcagatt aggaacagag atcgtatatt cactatctaa
    41701 aatcatactg tattgataag tagcaaatag gcagagcaag gcaaaaagta aaataaaatc
    41761 aatagctaaa aaaatgagcc ctttgatttg aacagataaa tata
//
LOCUS       DAZKCY010000012        41792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000012 DAZKCY010000000
VERSION     DAZKCY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.012"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(383..2764)
                     /locus_tag="ACG8XC_000575"
     CDS             complement(383..2764)
                     /locus_tag="ACG8XC_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP9190298.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2754..3401)
                     /gene="recU"
                     /locus_tag="ACG8XC_000576"
     CDS             complement(2754..3401)
                     /gene="recU"
                     /locus_tag="ACG8XC_000576"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFP9190299.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3465..4004
                     /locus_tag="ACG8XC_000577"
     CDS             3465..4004
                     /locus_tag="ACG8XC_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFP9190300.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4094..4513
                     /gene="gpsB"
                     /locus_tag="ACG8XC_000578"
     CDS             4094..4513
                     /gene="gpsB"
                     /locus_tag="ACG8XC_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFP9190301.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4535..4902
                     /gene="rnpB"
                     /locus_tag="ACG8XC_000579"
     ncRNA           4535..4902
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8XC_000579"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5111..6277
                     /locus_tag="ACG8XC_000580"
     CDS             5111..6277
                     /locus_tag="ACG8XC_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFP9190302.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6323..7819
                     /locus_tag="ACG8XC_000581"
     CDS             6323..7819
                     /locus_tag="ACG8XC_000581"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFP9190303.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7900..8583
                     /locus_tag="ACG8XC_000582"
     CDS             7900..8583
                     /locus_tag="ACG8XC_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9190304.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9228..10418
                     /locus_tag="ACG8XC_000583"
     CDS             9228..10418
                     /locus_tag="ACG8XC_000583"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP9190305.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10520..11473
                     /locus_tag="ACG8XC_000584"
     CDS             10520..11473
                     /locus_tag="ACG8XC_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP9190306.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11542..12891
                     /locus_tag="ACG8XC_000585"
     CDS             11542..12891
                     /locus_tag="ACG8XC_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9190307.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13074..13397)
                     /locus_tag="ACG8XC_000586"
     CDS             complement(13074..13397)
                     /locus_tag="ACG8XC_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190308.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13526..14689
                     /locus_tag="ACG8XC_000587"
     CDS             13526..14689
                     /locus_tag="ACG8XC_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9190309.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14759..16108
                     /locus_tag="ACG8XC_000588"
     CDS             14759..16108
                     /locus_tag="ACG8XC_000588"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9190310.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16153..16584)
                     /locus_tag="ACG8XC_000589"
     CDS             complement(16153..16584)
                     /locus_tag="ACG8XC_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9190311.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16972..19614
                     /gene="alaS"
                     /locus_tag="ACG8XC_000590"
     CDS             16972..19614
                     /gene="alaS"
                     /locus_tag="ACG8XC_000590"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP9190312.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19814..20410
                     /locus_tag="ACG8XC_000591"
     CDS             19814..20410
                     /locus_tag="ACG8XC_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9190313.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20532..21680
                     /locus_tag="ACG8XC_000592"
     CDS             20532..21680
                     /locus_tag="ACG8XC_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9190314.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21718..22131)
                     /locus_tag="ACG8XC_000593"
     CDS             complement(21718..22131)
                     /locus_tag="ACG8XC_000593"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFP9190315.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22210..22641
                     /locus_tag="ACG8XC_000594"
     CDS             22210..22641
                     /locus_tag="ACG8XC_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFP9190316.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22740..22973)
                     /locus_tag="ACG8XC_000595"
     CDS             complement(22740..22973)
                     /locus_tag="ACG8XC_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9190317.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23156..24826
                     /locus_tag="ACG8XC_000596"
     CDS             23156..24826
                     /locus_tag="ACG8XC_000596"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFP9190318.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24973..25359
                     /locus_tag="ACG8XC_000597"
     CDS             24973..25359
                     /locus_tag="ACG8XC_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190319.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25346..26044
                     /locus_tag="ACG8XC_000598"
     CDS             25346..26044
                     /locus_tag="ACG8XC_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFP9190320.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26049..26513
                     /gene="lspA"
                     /locus_tag="ACG8XC_000599"
     CDS             26049..26513
                     /gene="lspA"
                     /locus_tag="ACG8XC_000599"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP9190321.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26510..27421
                     /locus_tag="ACG8XC_000600"
     CDS             26510..27421
                     /locus_tag="ACG8XC_000600"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9190322.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27656..28195
                     /gene="pyrR"
                     /locus_tag="ACG8XC_000601"
     CDS             27656..28195
                     /gene="pyrR"
                     /locus_tag="ACG8XC_000601"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFP9190323.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28209..29492
                     /locus_tag="ACG8XC_000602"
     CDS             28209..29492
                     /locus_tag="ACG8XC_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFP9190324.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29505..30431
                     /locus_tag="ACG8XC_000603"
     CDS             29505..30431
                     /locus_tag="ACG8XC_000603"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFP9190325.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30450..31730
                     /locus_tag="ACG8XC_000604"
     CDS             30450..31730
                     /locus_tag="ACG8XC_000604"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP9190326.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31735..32811
                     /locus_tag="ACG8XC_000605"
     CDS             31735..32811
                     /locus_tag="ACG8XC_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFP9190327.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32816..36001
                     /gene="carB"
                     /locus_tag="ACG8XC_000606"
     CDS             32816..36001
                     /gene="carB"
                     /locus_tag="ACG8XC_000606"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP9190328.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36055..36831
                     /locus_tag="ACG8XC_000607"
     CDS             36055..36831
                     /locus_tag="ACG8XC_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFP9190329.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36828..37760
                     /locus_tag="ACG8XC_000608"
     CDS             36828..37760
                     /locus_tag="ACG8XC_000608"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFP9190330.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37757..38473
                     /gene="pyrF"
                     /locus_tag="ACG8XC_000609"
     CDS             37757..38473
                     /gene="pyrF"
                     /locus_tag="ACG8XC_000609"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP9190331.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38480..39109
                     /gene="pyrE"
                     /locus_tag="ACG8XC_000610"
     CDS             38480..39109
                     /gene="pyrE"
                     /locus_tag="ACG8XC_000610"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP9190332.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39097..39411
                     /locus_tag="ACG8XC_000611"
     CDS             39097..39411
                     /locus_tag="ACG8XC_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190333.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39449..40090
                     /locus_tag="ACG8XC_000612"
     CDS             39449..40090
                     /locus_tag="ACG8XC_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFP9190334.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40133..40825
                     /locus_tag="ACG8XC_000613"
     CDS             40133..40825
                     /locus_tag="ACG8XC_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFP9190335.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40822..41724
                     /locus_tag="ACG8XC_000614"
     CDS             40822..41724
                     /locus_tag="ACG8XC_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9190336.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
BASE COUNT        13507 a         7352 c         9109 g        11824 t
ORIGIN      
        1 agatggtgca gacaaattat gataattaag catactatca gataactcaa aatgaagcct
       61 tctttcaaaa aattattcaa aaccatatat tgtatttttt atttgataat aacaatggga
      121 ttattaagaa gtggcactaa ataaaagact agtgtgtatt tagccgattt aattataaac
      181 aagtaaatgg ataaagtata tgtaatgata aaagaaaaat ggattttcta atttttaagc
      241 taaatgttag atcacacatg tatatgctgg atagtcttat aaaaaatcat gtaatacagc
      301 ttggtacatg atttttattt gtagctgttt tttacataga cacaaaaaag aaaccacgct
      361 tgtagacgtg gtttcttttt tactatctgt ttccagaagc tgaggtgctg ctggatggtg
      421 tatggtcatt tgcagcatcc gctccgctag ttgctggctc actggaagaa gcaggaggag
      481 cagatgaaga cggtgtcgag ctttcttcgg atgaagaaga aggaaccgtt gttgaagacg
      541 ctggctcgct tgtttctgct gtcgtgcttt cttttgagga ctcagcagtt gattcgcttt
      601 gactagtaga gctagagctt tgagtcgttg tctctgttgt tgagcttcca ggagtctgga
      661 catagctgga agatggaata gttgttgatg gtgtaattgc attcgatcta gctgtatatt
      721 gatctttgta gtacagttca ccgcccacac gaattaatcc gcttggcatc tcccagtctg
      781 tatttgtcac gttagctgaa acatactgca taagctcgcg ataaacatca gaggctacgt
      841 gtgacgattc tgaagtgaca ggtgtcattt ttttattgta acctgtccac acggaaatcg
      901 aataatttgg cgtatagccc gcaaatagga tatctggata caccccagaa gaaataccaa
      961 gttttgcata ctcgtcatct gtgtaattgg atgttccggt cttaccggct tgatataacc
     1021 cagcgatttg cgcattcgta ccagttcctt cagtgatcgt atcttttaaa atgtctgtga
     1081 tcatataagc tgtttctgga gacatggctg tttttccatc tggctcataa gtttcttctg
     1141 ttccgtcttg gaagacgatt ttattaacat attgaggttt gtaataagtc cctccattgg
     1201 cgaaagccgc ataggctgct gccattttta aggaagaagc tccgtatttt gtcccatctt
     1261 gttcttccgt attactagag atcgcattcg attgatggat cgtactgtat tcgatcccaa
     1321 gattttttaa gaaagaagcc actttatctg aaccgacttc attgaataat ttgactgctg
     1381 gaacattacg ggataagtaa agtgcttggc gtaaagtaat ggtgcccata tattgattat
     1441 cccagtttcc tacaggagtc gaagtccctt catagttata cggtgcgtca gtaatagttt
     1501 ttccagtaga atatttcaaa tattcaaaag ctggtccgta atctgtgaca ggtttcatcg
     1561 tagaaccaaa atcacgggaa gtattcactg ctaagttgtt gcctaaagtt acgtcttctg
     1621 cgatgtgacg tccgccaatc tgggctttta ctttcccggt attggtgtcg ataagggtgg
     1681 aggcaacttg catctcgtcg tctgggtaag agacatactg atccgtgttg acgatatcat
     1741 atagtttctt ttgggcgtcc agatccaaat tcgtataaat ctctaaaccg tctgtgtaga
     1801 cattcttatc tgttttttct tgtacttcat tgatgacttc tttgacataa ttatcgacga
     1861 tcttagtatt gtcatcggat tgagttagtt cttgcagacc atccgttacc ggtacattaa
     1921 cagcttgatc atattctgtt tgagagattt tttcgttttg aagcatcgta tacaaaacag
     1981 tatctcgtct tttcttcgcc tgatctggat aaacataagg atcataggcg gaaggggctt
     2041 gaggcatccc agcaagcaat gctgtttgcg gtaaagaaag ttctgataat ttcttaccaa
     2101 agtacatctc agaagctgtc tccatcccgt acaagccgtt tgacatatac actttattta
     2161 cgtagtaggt caagatctct tgtttcgatt ttttttgttc caatcttaca gccatccacg
     2221 cttcttgggc cttgcgtttc agagtctgat cctcggcact ggtggagaag aaggaaagtt
     2281 tgatcaattg ctgtgtcaaa gtacttccgc cttgcagtcc gccagaggta aagttagaga
     2341 gggcagaacc gatgatccga atagggtcaa ccccgatgtg tttataaaaa cggcgatctt
     2401 ctaccgacac gatcgcatct tctaatgttt tcggtaattc attcgcagaa attttttcac
     2461 gcttttctgc gcctaaatct tcgaatagtt cgccatcttg tgtatatagt ttggaagata
     2521 cggtagcgtc taatttttta tccgtcaatt cgggcgcatc ttttgcgtaa taccagaaaa
     2581 gtcctacacc tgctaaaaag aggatacaga aaaatgagag tattcctaaa aggatcttga
     2641 taaataatcc ttttttatgt gttccagagg actttccaga acctttgcca gaattcttct
     2701 tcacactctt tttaggagta ggctgtttct gtcgtctaga gcttctagtt tgttcatttg
     2761 ccattcttgc tttctccttc aatagataat gttgataaat agaagtcaac cgcgtccaaa
     2821 taaggaatcc gcggagaaat accagttttg atctctattc ctagttcttc gatcaaagat
     2881 aaaggtagtg atttgcgtcc tttttccttt tgggcgtccc agtaagcaat caaatgaaca
     2941 ctattcaaat aaaaacagcg cttcaaagac gaaaaccata acaagacaaa gcagacccca
     3001 ttttgttcga ggcatgcttt catatgttcg atttgatggg catggaagtt tttaaaggga
     3061 aaagatgttt tgttcttggt ttcttttgct tcaaagtcca agtaaaagcc tcgatagacg
     3121 ccattatagt ctgtagttga ggcttgggta aagtaggctt ccttgatcac agcagcactt
     3181 ctcttaggat agtctacttt cacgatttgg ataggggtag gctttttatg gataaccgcc
     3241 aacccgcggg aaagataata tttattactt tcattgattg cttcttcaaa gcgcatcccc
     3301 cgattgccga attcagtgat ctgatttctt ttttcttgaa ttttttcgac gggagaggca
     3361 ttcttataat aaggttggcc atttggatag cgcaatacca tctttaatca cactcctagg
     3421 ccacattata gcaaaaaagc ggatagaaag aaaaggaatc tgagatggat aagtcaaaag
     3481 taatctatgt gtctgggtat cgaagtttcg aactcggtgt ttttaaagaa aatgatccta
     3541 aaatccaagt tgttaaaaat gttttaaaaa aagaactcca tcagttagca gaagaagggc
     3601 tggaatgggt acttgtcagt gggaatttgg gcgtcgaact atgggctgtg caaatagtag
     3661 cggaattgaa agaagaatat ccggaactat ctcttggtat tttgtatcct ttttctgatt
     3721 ttggtaataa ctggaatgaa gggaaccaag aaaaaaaaca attagcagaa caattagcag
     3781 attatgtcga agctgttagc catcaaccct accaatcacc tagtcagcta aagaatcaca
     3841 ctcgttttat acttgagcat acagtgggct gtctgctgat ctatgatgaa gaattccctg
     3901 gaaaaacgaa gtattttttt gaagatgcac agaattacca agcagatcat ccctacgaaa
     3961 ttcggctgat cagtatggac gatcttcaaa attttagtga ataatcgata ttgtgtttga
     4021 tttatcaact tttttcctat acaatgaaaa aggtagaaat gttattacgt aaatgaaaac
     4081 tagaggtgta aatatggcga atttggttta tagtcctaaa gacattttac aacaagaatt
     4141 caaaacaaaa atgcgcggct atgatcccgt tgaagttgat gaattcttag acaatatcat
     4201 caaagattat gaaacttaca gcaaagaact ccttgcttta caagaagaaa acgatcgatt
     4261 gagtgcaaaa gtagctcaat tatctaaaac tcaaggagca gctcaaacac gcgtgcaaca
     4321 aacagaggca ccaaagagcg cagcagtgac caattttgat atcttaaaac gcttgtcaaa
     4381 tcttgaacgt gaagtatttg gtaaaaagct tgatcaacaa gcaagtgcag tcaaaccagc
     4441 acaacctaat ccaaataact acacaaatgc agacacaagc ttagatgata atgaaaaaac
     4501 acgtcaattt taatttttga ttaatgaatt tgcggttttc gggtaatcgc ggtctgactt
     4561 gatcaggctg aggaaagtcc atgctcgcac aggctgtgat gcctgtagtg ttcgtgctta
     4621 gcgaaaccat aagctaaggt actctttgga gtaacggcag gaaaacaagc taaggcttcg
     4681 gctatgctta agtatccttg aaagtgccac agtgacgaag cggctgggga aacccatccg
     4741 gtggaacgcg gtaaacccct cgagcgagca acccaaacaa tggtaggggc gctttttgtc
     4801 tggaagaaga acaggacaaa aaggcagtat aactgcagat agatggttac cgctttgttt
     4861 tttctccctg gaaaagcaaa gtacagaaca tggcttatag aaaaccgcaa acatcaggga
     4921 attaggtcgt gacaaaagta atgcgttata agaaacaaga aaaacatttg aaagctattt
     4981 ttcaatactt cagatgaatc gttcttatta caaaacaaac ttttgtatca ccggttattc
     5041 ggaatttgag taaaagtgtg gggtaggact gatgtcccac ctcctttttt tataaataag
     5101 gagattgtgt atggaaaaaa acaggaagtt caacctcgtc gcaactgcgg caagcgggct
     5161 agaagcactg gttggtaaag aattaagaga tcttggaatc gaatgccaag ttgagaacgg
     5221 acgtgcacgt tttgaaggaa caatggaaac gatcgctaca gctaatttat ggttacgtac
     5281 agcagatcga gtaaaaatcg tggttgggga attcgatgcg tatacgtttg atgaattgtt
     5341 tgaaaacgta aaagcattgc cttgggaaga ttttcttcca ctagatgcag cttttcctgt
     5401 agctggaaaa tcgatcaagt cgaaactata tagcacacct gattgccaag cgatcacaaa
     5461 aaaagcaatc gttgaacgtc tccgtaccta ttatcaccgt ccagcttctg taccactgac
     5521 ggaaactggc gcacattttc agttggaagt agctttgctg aaagaccatg tgatggtaac
     5581 tctggatacg acaggaccaa gtttattcaa acgtggttat cgcttagaaa aaggtggagc
     5641 accattgaaa gaaaacatgg cagcagcact tattatgttg acaaattggc gaaaagaccg
     5701 tccattttat gatcctgtat gtggttcagg tacgctttgt attgaagcag ccttaatagg
     5761 ccataatatc gcaccaggat tcaatcgtga gtttgcttgc gaatcttggg attggttcag
     5821 tcaagaaatc atggaaaatg ttcgatcagc agccgaagaa aaagcagatt atgatattga
     5881 attagatatt actggttcag atatcaatgg acggatgatt gaaattgcca aagccaatgc
     5941 agaagaaatc ggattaggct cttcgattac ctttaagcaa caagcagtca aagacttcaa
     6001 aacggataaa gaatacgggg tcatcgtggc taatccgcct tacggagaac gtctaggaga
     6061 agaagaaact gttcggaaat tatataaaga aatgggcgat gtattccgtc cattaaaaac
     6121 atggagtaaa tatatcttga ctagcgactt agcgtttgag gaattttacg gacaaaaagc
     6181 aaccaaaaaa agaaaattgt acaatggtgc attgcgtacg gatctattcc agtattgggg
     6241 aactcgtccg cctagaaaaa aagagaaaga cgaataatct tactaattga ttcattcaat
     6301 tggatgtttg gaggaatagg gaatgaaaga aaaagagttt ttacaagaag taaaagaaat
     6361 caatcttcta caacaagctt taggaatcct cgattgggat acacaaacag gtatgcctga
     6421 aaaagctagt gcataccgaa gtgaagtaga cagctatctt tacagcattt atttttcaaa
     6481 aaaaatcggt ccaaaaatca aagaagccat tcgctatttt tctgaacatc cggaagaatt
     6541 gtcggaagtt ggaactgccg tttttgccaa agtaaaagaa gagtatgatt tggaacatcg
     6601 cgtaccggaa gatcttatga gaaaatatac agctgccgtt tcttcagcac acggacaatg
     6661 gcaaaaagct agagaaacac aacaattttc tgattttcaa cacgcattga atcaaaatat
     6721 tgaattaaca aaacagttga taccttactg gagaaaagat gaaaagacga attatgatgt
     6781 tttgctgaat caatacgaac ccgggatgac tgtcgaaatc cttgataagg tatttgatca
     6841 gcttaaagaa ggaattcttt cgatccgccg tacgctccaa gaaaaaggga ctgaacctga
     6901 aacagacttt ttaaatcgga gaatgacgaa aaaacaacag aagagatttg tagtgaaagt
     6961 aatcgaacaa cttggttatg attttagtcg tggtcgttta gatgacactg tccacccatt
     7021 tatgactgga attaatccga atgatgcacg aatcacgaca cgatggaacg aaacagattt
     7081 caaaatggct gtttttggag tgatccacga ggctggtcat ggtatgtatg aacaagacat
     7141 cgatgataaa tatgcgtata caccggttta tgaaggtaca tcaatgggta tccatgaatc
     7201 acaatcgttg tttaacgaga tcattattgg aagtagtcgc agcttctgga aaaagcaata
     7261 tcctttcttt caagaatgtg cagaaggaac attcgatgat attccttttg acaaatttta
     7321 tcgtttattg aaccatacaa aatcaagttt ggttcgtatc gaggcagaca gtttgactta
     7381 ccctctccat atcatcattc gttatgaaat cgaaaaaatg attttcaatg aaggggtaga
     7441 tgttgctgaa ctacctgaaa tatggaatca aaaatatgaa gagtatctgg gaatacgacc
     7501 aacaaatgat gtggaaggca tcctacaaga cgttcattgg tcaggtgcaa gctttggtta
     7561 ttttccctct tatgcattag ggtacatgta tgcggcacaa ttacttcatt ctttgcagca
     7621 agaaatagat gtggaaaatg ttctagcgtc agacgattat tctccaatca aaaactggat
     7681 gtcagagcat atccatcaat atggtgcatc tagaaaacca aatcaattaa tcatggatgc
     7741 aacacatgaa ccgcttaatc cgcaatacct gatcgactat atgaaagacc tgtattttaa
     7801 tgtatatcag gtaaaataaa atacaattta caaagatagc tgaaattcat ctaatctata
     7861 gatgttttca gctatttact taagaaagaa gtggaacgaa tggataaatt acaagtgatc
     7921 gctgattata gtatggacca attagcgcat gatcaaacag gacatgggag cgatcatacc
     7981 aaaagagttg tcaagttagc tgaaaaaatt ttggagacgg aaccgcaggc agaccgcttt
     8041 gtgacgcttg cagcagctta cctacatgat acgattgatg ataaagtcgt taaaaatgag
     8101 aacgaggcaa agcaacagct tcgtgtattc cttcgctctt tgccaatagt agaggaacag
     8161 atctcgatga ttttttccat tatcgaaaat atgtcctttt ctaaaaatct ctctgaagcg
     8221 gccgaactat ctctggaagg taagatcgtt caagacgctg atcgtatcga agcattagga
     8281 gcaatcggca ttttgcgtac tgcttatttc ggcggtggac atgggcaccc gatttttgat
     8341 cctgaactgt atccgcaaac attcaaggac aaagaaaatt accgaaaagg cacgacagtg
     8401 atcaatcatt tttatgaaaa actgttcttg ctgtctgata aaatgaatac tgattacgga
     8461 tatgaagaag ccaaacgaag agaaaccttc atgcgtgaat tcttagaaga attttttctg
     8521 gaatggtcta ttgacgatga aaattttact ccagacaatt ggatcaaaaa taacaaaaaa
     8581 tgatggaggt tgtgacagaa gtggtcagct ccaagatatt tcggatcatt ttccgtaaaa
     8641 gttacttctg gaacactgtt taccaagagg tcgtgagcct gccgttcagc tccgagtcac
     8701 aaggaacaat ttcaaaaaat agcttctcgt gtttgatgaa tattgtaact tgtgattcgg
     8761 aattggcagg agaacaccgt ttattcggaa gaataggact gtgacaagac ttttgtcaca
     8821 gtcctttttt cgaattcaac aagtaaaact tttaagaaat attcagagag taaaattaaa
     8881 aattgctgat aaacgaagat ggaaatttta tgtattttta ttggtctatg caataaataa
     8941 attaaatatt cagaatacaa ttgattttaa cgaaagggct tctatcaatg gttgtgttgt
     9001 atttatctgc ccaggtagag taaaatagat cattatctaa aaggtaattg ttcatactat
     9061 ataagtcatt tgaaagtaaa aagatactgt aaataaaaaa tgtggttaaa taattctcat
     9121 aatcagtgta agcgttatca aaaatatagc atacttgctt tgtagatgaa tgacacgata
     9181 tatgtttggt atgttctgca agacagatga atatggaggt ggagacaatg tcttccaaag
     9241 aactaacaat gtatttagag aaagaattgg atcaactgaa agaaaagggg ttatacaata
     9301 cgatcgacat actagaaagt gaaaatggtg cttgtatcat cgtagacgga aaaaagatga
     9361 tcaatctagc atccaacaat tatcttggat ttgccaatcg cgaagaattg aaaaaagcat
     9421 gtattgaagc aaccgaaact tatggtgtag gagcaggagc agtccgaacg atcaatggtt
     9481 cgctgaaaat ccatcagcaa ttagaagaaa aaatcgcaga attcaaaggg acagaagcag
     9541 caatcgcctt tcaatctgga ttcaactgca atatgggcgc tatctctgca gtaatgacaa
     9601 aagaagatgc tatcttatcg gatgaattaa accatgcatc tattattgac ggttgccgct
     9661 tatccggagc aaaaatcatc cgaattaaac atcaagatat gaaagacctt gagaaaaaag
     9721 caaaggaagc gatagaaagt aagaaatata aaaaaatcat gtatatcaca gatggtgttt
     9781 tctccatgga tggagacatt gcacgattgc cggaaattat tccgattgtt gaaaaatatg
     9841 gactgatcac ctacgtcgat gatgcgcacg gctcaggcgt aactggaaaa ggtgcaggaa
     9901 ccgtcaagca ttttggatta agcgacaaaa tcgatatgca aatgggcacg ttatcgaaag
     9961 ctataggtgt tgttgggggt tatgtagctg gatcgaaaac actaatcgac tggttgaaag
    10021 ctagaagtcg gccattccta ttttctactt cgctaactcc aggtgcagct gcagctgcat
    10081 tggcttcgat tactttgatg caagaacatc cggaacttgt cgaaaaagtt tgggaaaatg
    10141 ctaattattt taaagaagaa ttgaaaaaag taggctataa catcggtatg tctgaaacac
    10201 ctattactcc agtcattttg ggagacgaaa aagtgactca gactttctct aagaagctga
    10261 tcgaacatgg gatttatgct aaaccaatcg tttatccaac tgtgcctctt gggacaggca
    10321 ggatcagaaa tatgccgact gcggaacata cgagggaaat gcttgatgaa gcagtagcag
    10381 tttatcaaaa aataggaaaa gaaatggaaa ttatttaact aaatttagtt ttttcgtcta
    10441 tttaaaatta gagaacatgt tcacgcatat attgttaatc atctgaagat agtagtaaaa
    10501 aagatcaggg aggatcatca tgaaaaaagt attggtgaca ggatgtttag gacaaattgg
    10561 ttcagagttg atcacacggc tacgtcaaga actaggcagt gaaaatgtca ttgcgacaga
    10621 tatacgcatg cctgaagtca ttgagaaaaa cgatatcttt gagatcctag atgtaatgga
    10681 ttacgaaaaa atgcgcagca tagcagaaca atatcaagtg gacacattga ttcatttggc
    10741 tgctcttttg tcagctgttg cagaagcaaa accacaatta gcctggaatt tgaacgtgac
    10801 tgggctggtc aatgcattgg aagtcgctag agaattccag ctgaaatttt ttacgccaag
    10861 ttcaatcggc gcgtttggtc caaatactcc aaaagatcat accccacaag atactcttca
    10921 gcgtcctaca accatgtatg gggtaacgaa ggtagctgga gaactactat gtgattatta
    10981 ttatgtgaaa tatggcgtag atactcgtgg tgtccgtttc cctggattga tttcttatgc
    11041 aaccttgcca ggaggcggta cgacagacta cgcagtcgat atttattatt cagcaatcaa
    11101 agaaggaaag tacactagtt tcattgatca aggtacatca atggatatga tgtatatgcc
    11161 agatgcgatc gatgccatta taaaactcat gaatgcagat cctgatcgat tggttcatcg
    11221 aaatgccttc aatatatcgg cgatgtcttt tgacccggaa gagatcaaag catctattca
    11281 aaaaatcata ccagattttg aaatggatta tgatgtggat cctgtacgtc agtcaatagc
    11341 agaatcttgg ccaaattcac tagatgtttc ttgtgcgcaa gaggaatggg gcttttcgcc
    11401 gaaatacgac ttagatgtga tgacaaagga tatgttggaa aaactgaaag aaaaattggt
    11461 agagacggta taaaacaaga accacatcca aaaagttttt tatacttttg ggattggcta
    11521 tggtatgaaa ggggacaagg gatggaagaa tatcaagcca cgccagtaaa agaagtaaaa
    11581 atagaaaatg aattaaaacg tacgatggga ttttttactg ctttatcgac tgttatgggc
    11641 acagtgatcg gtgcaggggt attttttaaa gccgcaagcg tagcagaagt tacaggatcc
    11701 gccagtttgc atatgttttc ttggtttctc ggtggtatga tttctgtctg tgcaggacta
    11761 acaggagctg aactggcagc tgctattcca gaaacaggag ggatgattaa atatatcgaa
    11821 cggatttacg gaaatacagc agctttttta ttgggctggg cgcaagtcgt gatttatttt
    11881 ccagcaaatg tagcagcctt gtcgatcatt ttcggtacac agttcgtcaa tctatttggt
    11941 ctttcccagt caatgattgt tcccgtagct gttacagctg ctgtatcgat cctgttgatc
    12001 aattttttag gttctaaagc tggaggggct tttcagtcga ttacgcttgt ctgcaagctt
    12061 attcctttgt ttgtgatcgt cattttcggt ttgttccgac aagaaggagt agacttccaa
    12121 ctgtttccga tccaagcagg agaaaatctt tcatttttct cagccttggg agcgggcttg
    12181 ctagcaacga tgttcgcata cgacggttgg atacacgtag gaaatatttc gggagaattg
    12241 aaaaagccgg cgaaagattt gccaaaagca atttctttag gaatcatcgg aatcatgatc
    12301 gtttatttat tagtaaatgc ggtgttctta agaaccgcat ctatcgacgg agtcgcagga
    12361 aacagcaatg cggcaagtga cgttgcaaaa atgatttttg gcggcttcgg tggaagactg
    12421 gtcacagttg gtatcttgat ttctgtttat ggaaccatca atggctatac ccttactgga
    12481 atgcgtctcc cttatgttat ggcaaaagaa aacaacctgc cttttagcaa gctctttgct
    12541 aaacttcatg ataaaactaa agttcctgta gccgcaggta tcttagagct agtcatcgct
    12601 attggcatga tgatgatcgg tggttttgat acactaacgg atatgctgat ctttgtcatt
    12661 tggatttttt atacgatggt ctttgttggt gtcattcttc ttcgtaaaaa agaacctgat
    12721 ttgtttcgac catacaaagt tccaatgtat ccgtttattc cactagtagc aatcattgga
    12781 gggactttta tcgtatcaag cacgcttatc actcagacct ttttagcttc gatggggata
    12841 gtattgacac ttgctggtat cccgatttat ctctatttaa aacgtagata gctaaaacat
    12901 tcgaatttta ttagaactgc acttctctgc caaacaaaat attttattta aatgaggagt
    12961 acgtggaaga aatgtcaaaa gtactcgtta attttgatca cgtaaatgtc gttttgtcta
    13021 cgatgaaata gcaaagtaaa agacagaaat cgggctcaac cgatttctgt cttttacttt
    13081 gatttgcgta actgaactat tcggcgtacg ccgcgataaa tatttaataa aaccaagcaa
    13141 ccagaaatga aaatgggaag ccaaatcaga tagacagggt agccaagcac tttagacaaa
    13201 gcatgtggag tcattttatc catttcaaga agtgcttgct gccaagtctg aagattgaag
    13261 actagcatga aacccgaaat aatcaggaga ataaatccta aataatgaat tgtagttctt
    13321 tttcttacat aagcgatcgt agcataaagg aacagtaaaa aaataggtac gccaaaaaca
    13381 aagtacatat tcagcatctc gacaccccct ttgtgtttat cttactttac tttgaaagaa
    13441 atgagaaata atagcctcgt tacttgcttg aaacagcaaa ggactgctaa gataagagag
    13501 atacaaagaa tcaaggagga gtcatatggc aagttatatc gaaccgattc gttttgcgct
    13561 gattgttttt ccatttttag ccttagcgat ctcatctgtt ttttttattt atgaatatcg
    13621 aaagtatgga acatttctag taacaagggc agttattttg tattctttcg ttttttattt
    13681 attgtgtgcg tattttttgg tgatcttacc acttccggca aaggagacgg ttgcccaaat
    13741 gacagggcca aagatggagc tgcatttatt tgcttcatgg caacatttca gagacaacac
    13801 ggtattggtc ttgtctgaac ctagtaccta cctgccggca atgaagcaga gtgtattttt
    13861 ggaaccagct tttaatgttt tattactcat accatttggc atttatttaa gatattattt
    13921 ccggctttct tttatgaaaa cgatgattgc tagtttctgc ctttctctat tttttgaatt
    13981 gacgcagcta agtggacttt attttatcta cccaagacca taccgcttat ttgatgtgaa
    14041 tgatttagtg acaaatacat tgggtggaat cattggtttt gtcgttacgc cgttatttac
    14101 atttatgctt cctacaagaa gacgaatgga tgaactttct tatgaaaaag gaaagactgt
    14161 ctcattgact cgacgcttgg tcgcttattt gatcgactgg acatttttat ctcttattaa
    14221 tttggtagcg gcgattttct ttaaaattgc gttcgattca ggggcagtta cttctcatat
    14281 ctggtggctt ttattagaag taggggtata ctttatgatt gtaccgttta taaccaatgg
    14341 atggacactt ggcaaaaaaa ttgtgcgtat ccgtttggtg gaagaagacc gcgaacgaat
    14401 ttctttaaaa gcattgatga tccgctacgg gtatctgtac tttctttttt atggtttaag
    14461 ctggttgggg cagttgcttc agcaaatcat gaattcgaca acgaatacgg tgttgcagat
    14521 gagtgcagta ttcatttctc tcggtgtctt gttactacaa ggtattttta ttttaaatat
    14581 cttgctatca tttttgagaa aaaaacggat tctgttttat gaaaaagcta gtcacacata
    14641 tactgttagc acgattggcc aaacaaagga tgatgattct cgaaattagc tgagcaaaaa
    14701 cgctttgtct gtgttataat gccaaagtcg tgataattta aagagaagag gtgcacgaat
    14761 gaataatttt gatatattcc gcttcaaacc gtttatcaat gaagcgttaa ccgcaaaggg
    14821 cttcaaacag cccacagagg tccaggaacg tttgattcct ttaattgcaa aaggaaaaaa
    14881 tatcattggg caatctcaaa ctggtacagg aaaaacacat acgtttttgt tgcctttgtt
    14941 tgaaaaaatc aatgcagaaa aagaggaagt acaagttgtt attacagctc caagtaggga
    15001 actagctgca cagatctatg aagcagctac acaaatcgct aaattttcta caccggagat
    15061 ccgtgtctcg aattttgtcg gcggtacaga caaacagcgt caattaaatc gtctgcatca
    15121 ccaacagcca caggttgtag taggcacgcc tggtagaata ttagacatga tgaatgaaca
    15181 agcgctgaag attcacaccg catttgcatt tgttgtcgat gaagcggata tgacattgga
    15241 tatgggtttt ttaaatgaag tagaccaaat tgcaagtcga ttaccagaaa agctgcagtt
    15301 tttagttttt tctgccacta ttccagaaaa acttcgtcca ttcttacgaa aatacatgga
    15361 aaatccaatc attgaagaaa tcaaaccaaa agcagttatt tctgaagaca ttgaaaattg
    15421 gctgatttca acaaaaggta aagatgagaa tcaaatgatt tatcaactgc tgacaattgg
    15481 tcatccttat ttagcaatcg tatttgccaa tacaaaacag catgtggatg aaattgccga
    15541 ttacttgaaa aaccaaggct tgaaagtagc aaagattcat ggagacatca ccccaagaga
    15601 gagaaaacga gtgatgcgac aagtacagaa tctggagtat caatatgttg tagcgactga
    15661 tttagctgct agagggatcg atatcgaagg tgtctctcat gttattaatg cagaaattcc
    15721 agaagatcta gacttcttta tccatcgtgt cggacgtact ggacgtaatg gcttaaaagg
    15781 aacggccatt acgttatatt cgccaaatga tgagcaagcc attgatgcga tagaaaaatt
    15841 gggcgtctca tttgaaccaa aagaaatcaa aaacggggaa atcgtcaaaa catacgatcg
    15901 taatagacgt gccaaacgag aaaaatcaaa agatgtatta gatcccacgc tgattggtct
    15961 tgtgaagaag aaaaagaaaa agatcaagcc tggttataag aaaaaaatcg attgggcaat
    16021 tgcagaaaaa aataaaaaag aacgcaaaat cgaacgcaga cagcaaagcc gtactgctcg
    16081 taaaaataag aaaaattcac ataaataaaa aacaagaggt tgtgacaaaa ggcgtgtcac
    16141 aatctcttgt ttttaagcta gacttttttt cattccgata caaagtactt catcttcaat
    16201 aaaaggttct gtgcaagtat gatacccttg tttttcataa aaatctacag ccgtcatttc
    16261 agctactaca taactagtct gacaaccttc tgaaaccgcc cgtttttcag cagcttcaag
    16321 caactgaccg cctaaccctt ttcctcggta ttcttttgct acgcaaaatc gttccggctt
    16381 gaagcaagta ggtgttttct tttcataacg caaagtagct actggtcttt tttgatcaag
    16441 aaatagataa taggtttgtt gcgggatatc ttttccgtcg aattctagtt ctgcaggaat
    16501 cccttgttct aatacaaaaa cttgatatct caaaaagcag ctagctgcgc gtatccattc
    16561 atccgctcca actatttttt tcatcatatt cctccttgtc atgaatagca gagatgtatt
    16621 gattccttta ttcttatcgt accaaaaatc gtggagtatg accaaaagga aatgaaaata
    16681 aaaattgaga tattgacagt gatcaataga atgcgtatac taattaagta cgaaaaggat
    16741 atgatttgtt ttttaagact cacagaaaat tctttcttgg ctgaaaaaag aatagtcact
    16801 aatcaaattg ctcccttttt ctattttggt agaaataccg acgcagctca gcgttatcga
    16861 gtttaagagg taatgattac acatcattgc aatcaaggtg gtaccgcgag ttttcgtcct
    16921 tggctgtgat ggtgtttttt tgtatttaat ttatagaaag caggtaatga catgaaacaa
    16981 ttaagtagtg cacaagttcg acaaatgttt cttgatttct tccaatctaa aggacatacg
    17041 attgaaccta gtgcgtcttt gatccccgtg aacgatccaa ccttattgtg gatcaactct
    17101 ggtgttgcaa cattgaaaaa atattttgat ggttcagttg tgccagaaaa tccaagaatc
    17161 acaaatgccc aaaaatctat tcgaacaaat gatatcgaaa atgtcggtaa aacagcacgg
    17221 caccatacga tgtttgagat gctgggtaat ttttcgattg gtgactattt caaaaatgaa
    17281 gccatccact gggcatggga atttttgact gatccaaaat ggatggcatt cgatccagaa
    17341 aaactttatg taacagttta tccaaaagat acagaagcaa aacgcatctg gcatgaagaa
    17401 gttggcctgc cactagatca cattattgat gtagaagaca acttctggga tatcggtgca
    17461 ggcccatgtg gtccggatac ggaaattttc tatgaccgtg gagaagctta taacgattta
    17521 gcagaagacg atcctgaaaa ctatccagga ggagaaaatg aacgttattt agaaatctgg
    17581 aacttagtat tttcagaatt caaccataag cctgataaca catttgaacc acttcctcat
    17641 aaaaacattg atacggggat gggattggaa cgcatggtct caatcgttca agatgcgtca
    17701 acgaactttg agacagactt gtttatgcct attatccacg ccgtagaaaa gctaagcaaa
    17761 caagtaaaat atggtgaaaa cccagtaaca gatatttctt acaaagtaat cgcagatcat
    17821 attcgcgccc tttcatttgc tattggcgac ggtgcattgc catcgaatga agggagaggg
    17881 tatgtcttac gccgtttgct tcgtcgtgct gtcatgcatg ggaaaaaatt aggtattgaa
    17941 caagcattct tatatcgttt agtccctgtt gttggggaaa tcatggtaag ttattatcct
    18001 gaagtacttc aacaaaaaga atttattgaa aaagttatcc gtacagaaga agaacgtttc
    18061 aatgaaacaa tcaatgaagg tttggaaatc ttaaatcaag tgatccaaga agtaaaaaac
    18121 gaaaacggga cgactctagc aggaaaagac atcttcaaac tatacgacac atatggcttc
    18181 ccggtagaat taacggaaga ggtggcagaa gatgaaggac tgaaagttga tcatgctggt
    18241 tttgaaaaag aaatggatgc acaacgcgaa cgtgctcgct ctgcccgcag tactgaaaaa
    18301 tcaatggggg tacaatctgc tttattgaca gatatcaaag tagaaagtaa attcattgga
    18361 tacacagaac aagaatcaga tagtcaatta ttggtcatcg ttaaaggtga gtctcttttg
    18421 aatgaagtcg cagaaggtga agctcaaatg atctttgatc aaacaccttt ctatgcagaa
    18481 atgggtgggc aagtagcgga tcaaggatgg atcatgaatg aagtaggcga aatcgtagca
    18541 gttgttacag atgttcaaaa agcgcctaat ggtcaattta tgcacaccgt agaagtaaaa
    18601 gcaccaatga gtgaaggaca aacctatcat ctattgatcg atcaatcgat gcgcaatcgg
    18661 atcatcaaaa accatacagc aacccatcta ctgcacaagg cattgaaaga agtacttggc
    18721 gagcatgcaa atcaagctgg ttctttagtc gctcctggac atttacgatt cgactttacc
    18781 cactttggtc aaatcacttc agatgaatta gctgaaatgg aaagaatcgt caacgaaaaa
    18841 atctgggaag ctatcccagt tgaaacaatt gaaacagata tcgatacagc caaaaacatg
    18901 ggtgcaatgg ctttgtttgg tgaaaaatac ggacatgacg ttcgagtagt aaatattggc
    18961 ggctggtcaa tcgaactgtg cggaggcaca catgtagcaa atacagaaaa catcggaatc
    19021 ttcaaaatcg tttctgaatc aggaattggt gcaggcgtgc gacgaatcga ggctgtaaca
    19081 agtaaagaag cgtacgaact acttgaagaa gaagaaaaac aactaaaaca agtagcaaca
    19141 attgttaaat cacctcaatt gaaagaagtt gtttcaaaag cggaacaatt gcaacagcaa
    19201 gtaagagagc tacaaaagga aaatgaacaa ttagctagca agttagcgaa tcaacaagct
    19261 ggtgatgtat tcaaagatcc agaaacgatc aatggaaaaa caatcattgc agcacaagta
    19321 aaagtgaaag atatgaatca gctgcgtcaa ttagctgacc aatggaaaca aaaaaatatt
    19381 tcagatattc ttgttttagc aaacgaacaa gaaggaaaag tcagtctgct agcggcaatg
    19441 acgaaagaat caaatgaagc tggactaaaa gctggcgact tgattaaagc aatcgcaccg
    19501 aaagtaggcg gtggcggtgg tggacgtcca gacatggcac aagctggcgg gaaaaatcct
    19561 gctggtattc cagatgcact taaagctgcg aaagaatggg ttgcgcaaca atagggcgtt
    19621 tacgtttgac agaaaaacaa aagttcaaac attgaacgaa acatgattat gggggcgaag
    19681 acaaacaagt cttgccctca tacttttttc aattggatgg cgcatctaat caaatggtcg
    19741 gattcggatg gtctttagtt gacgaaagtg acagaaattt ttacgattag tataaataaa
    19801 aaggggtgaa aaaatgaata gtagaaataa gacctttcga ctagtcttac gtgcgatctt
    19861 gttagcaatc atcatcgtac aagcgatggt tccttggctg gggtttattc cattaggatt
    19921 cattagttta acaattatcc atatcacagt aattattgct gcagtagtat taggcccaaa
    19981 agatggcatg gtgattggct tgttttgggg aatcgctaca attgttcgtg cctatgccat
    20041 gccaacaacg ccatttgata cattagtgtt cacgaatccg attatctcgg tagttcccag
    20101 agtacttgtt ggcttagtag caggcttggt tttccactgg atttatcaaa aaaataaatc
    20161 aataacgatc agctcagcct ttgcaggggt actgggttca ttggtcaata cagtcttagt
    20221 gttaggattc atgggattat tttatacagg tgcaacggca gatgcctatg gtgtagactc
    20281 tgctctttta tttaaaacct tagcaggggt tgcagcaatc aatgggatac cagaagcaat
    20341 aggtgcggga atcataacac cattactagc aaaagcgtta tttgcagcaa caccgttgaa
    20401 accagaataa tgaccatctt ttaatagtaa taggacgcac gatagataga aaaaggaaga
    20461 gaggagagga aaacaatcgt cctctccatc tttttacctg agcaacaagc aaactagaag
    20521 gggtaaaaaa cttggctaag aaacaagaag aaaaaaagaa acaaataccg caacagcgga
    20581 caaaaaaagg agtactagcg ggtatcttag gattggttac gaatctgatc ttgtttgtct
    20641 ctaagctaat gatcgggctt ttttcaggaa gtgtctccat tatggctgat gcagtgaaca
    20701 gtctatcaga tactgcttca tccatattga ctttgatcgg atttaaaatt gctgcaaagc
    20761 cagcagatca ggaacatcct tatggacacg aacgcttcga atatatcagt gggttatttg
    20821 tttcgatcat cattacttat gtaggatttc aatttttaga gtcttctgct gaaaaaatca
    20881 tccatccaga aaatgtctct ttaacaccca tcgtcttttt tgtcctaatc atttccatct
    20941 ttttgaaatt tgcacaaggc aaaatgtatc aggttattgc gcaatccatt gattctgaaa
    21001 cattgagagc tacagcaaca gatagttata atgatgtctt tactacactg gctgtattgc
    21061 tttcagcagc aatcgaatgg gtaactggat ggcgtattga tggatatata ggttttgctt
    21121 tagcattata tatcctttat agtggattga cgatgcttcg aaattttgta tacgaactat
    21181 taggaagtcg cccaacgcca gcagagattg aagaaatgga agagcattta aatgcttgcg
    21241 atgatatact tggctatcat gatttattgg tgcataacta tggacccaat aaacgctttg
    21301 catcagtcca catcgaagta gatgaatcaa tgaatttgaa tgtggctcat aagattattg
    21361 atctgataga aaaagacttt aaaaagtctt tggatgtgga acttgtctgt cacttggatc
    21421 caatgcctgt tgataacgaa aaatactata atatcttgcg gcagttaaag caaattcttc
    21481 ttgaaacagg tacagggctt aatattcatg attttagagt aattcatcaa ggaaaaacgc
    21541 tgcaatttga tgtagtggtc cctgagaaat tccagtttgt tgatacagaa ctagaaagaa
    21601 tcttacgtag tcaaatagaa gaaaaaatcg gagattacca attggaaatt acgtttgatc
    21661 acaattatct tttatattaa atgagaaact ataaaaagac tccttaaagt ttttaattta
    21721 aagagtcttt ttttctttta attgtttatg gagagcctga cgtgcaagat tatctgctcc
    21781 tttatttttg ttttcgggca accattgcag gatcaataaa gaaaattcgg atagaagttc
    21841 ttggattctt tgaaggtagg gaaggaagag ctcgttcttc gttgcgtttt tgtcgaccgt
    21901 agaaactagt gttttgctgt ctgaaaaaca gaaaatcgtt tcattctgta agtcgttttt
    21961 ctttagaaat tctaaagttt ttaaaaagac ttcgaactct gcttgatggt ttgtccctat
    22021 agaaagcggg atagatagct gttcttgttt accttgatag acaatcaaga ttccacctcc
    22081 actaggacca gggttgcctt ttgtagaggc atcaatgtat acttttaaca taaaaagaga
    22141 ttctcctttt tgttcaaact aatgttatgg taagattata tcattactga atcggtgaga
    22201 ggttagagaa tgaaaaaaaa tattcgatgg cagcctgaac tggcacatac gatcatctat
    22261 tggtcattga ccttgatcat tctgtttttt agtctgattc tttctttaga aaacacaggt
    22321 ccttattgga aaagcaattt tgttttaggt atttttcttg tttttctatt tctaggtttg
    22381 cggcgttctt ttattttaaa atcaaactct gtgattatac gttatgctgc tttttggaaa
    22441 gaccgagaaa tatctgtcgg acagattcga tctttgacgc caactaatac tggggtgcaa
    22501 atgatgttgc aagatgagaa agccccatct gttttcatga tgcgaaaaaa agatcaagta
    22561 gtcctttttg atcattttga aaaaaacgga cccaaatcag aatcaattcc ttatccttat
    22621 acgaaaaaaa gaaaaagcta attttaaaaa tcagaaccta aaaaaacgaa tttttacagg
    22681 aaaagaaaag agtgagacgt acggttcatc ccgtttagcg tctcactcta tttgtttatc
    22741 tattcatgca tactagtatc ggtagtctga acgttgcctt tcttgacatg ggcagcttgt
    22801 gttcctcgat tcccttcgat gatctcaaat tcgaccactt gattttcttc caaagttttg
    22861 aatccctctt cttcaatcgc agtaaaatgt acaaaaattt cttctgtgtc atcataactg
    22921 ataaagccgt atcctttttt gttgtcaaac cattttacaa ttcctgtggt caaagcactc
    22981 acgacctttc taaagtctga tcatagcctt cattataaag agtatttata agtacgtcaa
    23041 ataaattaaa aacctgtaat tttatgtttt ttttctgaat aaaaaaaggt ataattggca
    23101 tatcttaaca aagaagatat tttgttgaat aaagttcgtg aaaaggggta atcaaatgaa
    23161 gaatgatctg caaatttcac aagaaacaga gctagaaccg attgttgaag tggctagtaa
    23221 attagatctt aaggaagacg atttggaact gtatgggaaa tataaggcga aaatcaattt
    23281 ttctggaatc aatcgatcaa aggataacgc tgaggggcat ttgatcttag ttacttccat
    23341 caatccaaca ccagctggcg aaggaaagtc aacgatcact gtagggttag gagacgcgct
    23401 caataaaatc aataaaaaat ctgttattgc attgcgagaa ccctcacttg gtcctgtgat
    23461 gggaatcaaa ggtggtgctg caggcggagg atatgcacag gtattgccta tggaagatat
    23521 caacttgcat tttactggtg acatgcatgc tatcacaaca gctaataatg cattatctgc
    23581 tcttttagat aatcatattc atcaaggaaa tgaattgaac atcgatgccc gccgagtgat
    23641 ttggaaacgg gtagtcgatc tcaatgatcg tgaactgcgt aaagtaattg ttggcttggg
    23701 tgggccaatc caaggtgttc caagagaaga tgggtttgat atcacagttg ccagcgaaat
    23761 catggctatc ttgtgtcttg cggcagacct tgaagattta aaggctcgtc ttgcaaggat
    23821 cgttgtcggc tatacatttg accgtcagcc tgtgactgct ggagacttga aagcagaagg
    23881 cgctctggca ttactgctga aagatgcaat caagccgaat cttgtacaaa caatttacgg
    23941 aacaccagct tttgtccacg ggggaccgtt tgccaatatc gctcatggct gcaatagtat
    24001 tttagctacc aagacagcat tgagattagc agattacgtt gtcacagagg caggatttgg
    24061 tgcagatctt ggcggagaga aatttttaga tatcaaagta cctaacttga aaaaagcgcc
    24121 ggatgctgtg gtgattgtcg ccactgtccg tgcattgaaa atgcatggcg gaatgaaaaa
    24181 agatgaactt aaaaatgaga atcttgatgc tttgaaagta ggttttgcaa atttaaagcg
    24241 ccatatacgt aatatggaac aatatcagct acctgtgatc gtcgcaatca atgaattcgt
    24301 aacagataca gacaatgaac tcacactgtt ggagcacttg tgtgaaaatc aggggattct
    24361 tgccaagaga gcgagtgtat gggcagatgg agcagaaggc ggcgttgatt tagcaaaagc
    24421 tgtagtacga ttgattgata gaaaagaagc taattacaag ccattatacc gcctagaaga
    24481 aacgatacaa gaaaagacag aaacaatcgt caagaaaatt tacggaggaa atggtgttgt
    24541 tttcagcaaa aaagcgcaaa aacaaataga agagtttacg aagaatggat gggacaaact
    24601 gccgatttgt atggcaaaaa cacagtattc attttctgat gatccttcac tgctaggtgc
    24661 accggaagat ttcaccatca cgatccgcga aatcatccca aaattagggg caggattttt
    24721 agtagccttg actggagatg ttatgacgat gcctggtttg cctaaaaaac ctgctgcatt
    24781 gaatatggac gtgacaaatg acggagaagt gttgggattg ttttaagctt gattataatc
    24841 taccatcgaa ggagctgtat tttgactaca gcttcttttt ttagacgata gggaattaat
    24901 tttgtttaca ctaggatcat tctttttctt tattgtattt tgagctatac taaagcataa
    24961 taggagggta tgatgaaaac gacatttata agaaaaaccg gtttttacgg tatgggtagc
    25021 tcgattcagc taagaatcaa caatgaaaaa cgcagtgttc tcagtcatga acaaaagata
    25081 acgcttgatc tggaagtgcc ctttacgatg caggtcacct tttattggct aaaaagcccc
    25141 gtttatacag tgaaagaagc aaagcctgct tatgtgatca caatgaatta cttgattctt
    25201 caaatctatc cgtttttatt tctagcaact gggttggggg ccgttttcct acaaaattta
    25261 tttttcagtt tttttgcagt tattgtgttg attgcgttct ttttctatat aaaaaaccgg
    25321 gcatatttga tcaaggagga aaattatggc aaactctgat ctttttttaa tcagtttcaa
    25381 tcgaatcgaa aaatggatga gagacgagat gggaaatgca cgaaatatgg gatttaccga
    25441 acttgtcaga cgcctagctc aaagaaaaca attaatgatc agaaaatacg aagatgatct
    25501 tttacagctt gctcagttaa ggaatgcgat cgtccatgat cggatcgcag tcgattttgt
    25561 cattgcagag ccaaatgatt ggacagtcaa gcgaatccag ctgatcgaac aagaactgat
    25621 tcagcctgaa accgtgctgc ctcgatttgc aaaacgtgta acaggttttg aacaagatct
    25681 tccgttactt gaactgttaa agatagtagc agaaaaaagg tattcacaat ttccgttata
    25741 caataaagga aaatttgtag cattgattac tttacgtaat atcgggtttt ggctagcaaa
    25801 agaaagccaa aaaggtccgg ttgatttaac aaataaacga gcgttggatt tgatcatcca
    25861 aaacggaaag tacacaaatt atcattttgt tccagctaaa acacatattt acgaggttga
    25921 agcgatgttt cgtgagcaag ggactttgga agccgtactt atcactaaag atgggaatcc
    25981 cgatggtaat ctgctcggga tcgtacgacc tcgtgatatt tataaacaaa tagaaaagga
    26041 ttgatttttt gttaattatt tattggatat tcagcgcttt gctagtagga gtggatcagt
    26101 tggtcaaatg gtggatcact gataatctag cgcttggaga gacgaaaaat cttataccag
    26161 gtattttgtc actgaatcat attcgaaaca ctggagccgc atggagtatg ctagaaggga
    26221 agatgtggtt ctttactata gtgacactca ttgcagtcgt tgtgattctc acattgatga
    26281 ttaaaaatcg tgaaaaaggt aatcgctggt tcatgattgg attgagtttg attctagctg
    26341 gtgcgattgg caattttatc gaccgtattc gtcttggcta tgtagtagat atgttccaga
    26401 cagattttat gagctttccg atttttaacg tagcagatgt gacacttgta tgtggagtga
    26461 tttgtatgtt aatttatatt attttagatg aaaaggatca aagaaaaaaa tgacagaact
    26521 taaaacgacg attcaccatg aatctggtag gatcgataaa gtattgacta atttattcac
    26581 cgattacagt cgttcgcaaa ttcagctgtg gctgaaaaat ggagcggttt ctgtcaatgg
    26641 acagccagtc aaagcaaatt acaaagtaaa acaaggagac gagattactg tcttgatccc
    26701 cgaaccagaa acattagatg tggtcccaga aaatatcccg ttgacgatcg tttacgaaga
    26761 tgaagatgta gcggtgataa acaaacctca aggaatggtc gttcatcctt cagcaggtca
    26821 tacgcatggc acaatggtca atgcgctgat gtaccatatc aaggatcttt ctacgatcaa
    26881 cggcgttatt cgtccaggta ttgttcaccg aatcgacaaa gatacttccg gactgctaat
    26941 ggttgccaaa aatgaccagg cacacgaatc attagctcag caattaaaag ataagacctc
    27001 tttgcgcaaa tatgttgctt tagtccatgg agagattcct cacgaaaaag gaaccatcaa
    27061 tgcgccaatc ggccgttcga aggaaaaccg gaaaatgcag gcgataagag aagatgggaa
    27121 gtcggctgtc acacatttta ctgtattgga acgtttcgaa ggatttactc tggttgaatt
    27181 aaaactagaa accggaagaa cacaccagat ccgtgtgcat atgaagtata tcggttatcc
    27241 gttagctgga gatccagtat acggaccgaa gaaaactttg aaaggcaatg gtcagtttct
    27301 ccatgcgcaa atgttaggtt ttacgcaccc gactacaaac gaaaagatga tctttgaagc
    27361 acctcttcca gaagtttttg aaaaaacttt agaaaaatta agaaaaaaca ttgcatttta
    27421 atttagataa cggtatagta attaacaacg aaagcataaa acttaataga attcctttaa
    27481 gtttagtccc gtgaggctaa gaagggtggg agcaaggata cgtatggtgc gccataagta
    27541 gattgctcat tgacatagta tagctatcag tgtatcagta tacgctcagt ctaaacattc
    27601 ctcctgtcct cttagggcag gagttttttt atgcaaaaaa gtagggggaa tgaaaatgca
    27661 agcaaaagaa gtagtagatc aggtaaccat gaagcgagca ttgactcgaa ttacttatga
    27721 gattatcgag agaaaccata gtatccaaga tatcgtattg gtaggtatca aaacacgagg
    27781 aatctacatt gcatcaagaa ttgccgaacg cctgaagcag ctagaagata tcgatattcc
    27841 tgtaggagaa ctggatatca ctttataccg agatgacaaa aaagaaaatc cagaagaacc
    27901 ggaacttcat tcttctgaca tcccagtttc cttagaagga aaagaagtta ttttgattga
    27961 tgatgttttg tatacaggac gaacgattcg agcagcaatg gacgcagtaa tggattttgg
    28021 cagaccaaga aaaatttcat tagcagtcct ggtcgatcgt gggcatcgag aattgccaat
    28081 ccgtgcagat tatgttggga agaacattcc aacatctcgg gcggaagaga ttttagtaga
    28141 gatgcaagag cttgatggac aagatcgcat catgattttg aaagaggaag attagaggag
    28201 agagaaacat gtcggagaaa aaaggatttc ataatgatga cgtggtgtta gatatccacg
    28261 atcgcccaac cccaggacat tgggtaggat taagtttaca acacttgttc acgatgttcg
    28321 gagcaaccgt gttagtcccc atcttagtag gtatcgatcc aggtattgcg ctagtcagtt
    28381 caggactagg tacattggtg tacttattta ctactaaagg aaagatacct gcgtacttag
    28441 gaagcagctt cgctttcatt gcagcgatgc agcttttgat gaaaacagat ggttatccag
    28501 cgattgcaca aggagcagtc acaactggat tagtctattt gattgttgcg ctgatcatca
    28561 aaaagatcgg ttcagcatgg ctggataaaa tcttgcctcc aatcgtcgtc ggtccagtca
    28621 tcatggttat cgggttggga cttgcggcaa atgcagcaac aaatgctatg tataatcagg
    28681 gcacgtatga tttcaagtat attgctgtag cgctgatcac attaggatta acaattttct
    28741 tcaacatgtg gctaagaggt tttctcggat tgattccgat tttactaggt attattagcg
    28801 gatacatcgt agccttgctg tttggaatcg tggatactaa gccaatcgta gacgctgcct
    28861 ggtttgctgt tcctaacttt gagattccct ttgtacaata tgaaccaaag ctatatcttg
    28921 gagcaatcac aacaatggca cccatcgctt ttgtgacgat gacagagcat atcggccatc
    28981 ttatggtatt gaataaacta actaaacgta atttctttga agaaccgggt cttcataaga
    29041 cgttgacagg tgatggatta gcacaaatcg tggcagcctt tgtcggaggt cctcctgtaa
    29101 caagttacgg tgaaaacatt ggtgttctgg ctatcacacg tgtccatagc gtgtttgtga
    29161 tcggtggagc tgcagtattt gcagtcattt taggattcgt aggaaaatta agcgctttga
    29221 ttttaagtat ccctggacca gtcatctcag gaatcagttt tatcttattt ggagtaattg
    29281 ccgcaagtgg aatgaagatt ttaatcgaga acaaaatcga ttttgataaa aagaaaaatc
    29341 tgttgattgc ttcagttatc ttagtagtag gaattggcgg actcgtcttt gaagtaggca
    29401 catttacatt gtcggcaatg gcattagcaa ctgtacttgg catcatttta aatcttatct
    29461 taccagaaac ttcacgaagc gaagaaaact aaggaggaaa cattatgatc atcaaatcag
    29521 aacgtatcag cttaaagcat ttattgaccg ttgaggcact tactgatcaa gaagttatgg
    29581 gcttgatccg cagagggcag gaattcaagc aaggtgctaa ttggactccg caaaagcgcc
    29641 agtactttgc tacaaacctc tttttcgaaa atagtactcg cacacataaa agcttcgatg
    29701 tagcggagaa aaaactaggg ctagaagtga ttgaatttga agcaagcaca agctctgtcc
    29761 aaaaagggga aacactatac gatacagtac tgacgatgtc agcattaggc gtagacgtag
    29821 cggttatccg tcatggtgat gagaactatt atgacgaact gatacaaagt aaaacaatcc
    29881 agtgttcgat tatcaatgga ggagacggaa gcggacagca tccaacccaa tgcttgcttg
    29941 atttgatgac catttatgaa gaatttggtc attttgaagg tctgaaagtt gccatcgtag
    30001 gtgatatcac tcactctcgc gtagctaaat ccaatatgca gatgttaaaa cgtctaggcg
    30061 cacaagtatt cttctcaggt cctagagaat ggtacgacga tgaattcgaa gtgtacggac
    30121 attatatgcc attggatgaa ctactagatc aagttgatgt catgatgatg cttcgggtgc
    30181 aacacgaacg ccatgatgga aaagaaagtt tctcaaaaga aggctaccat caggaacatg
    30241 ggttaacagt tgagcgtgcg aagaagttgc aaaaacatgc aatcattatg cacccagcac
    30301 cagttaaccg tgatgtagaa ttagctgatt cattagtgga aggattacag tcgcggatcg
    30361 ttcagcaaat gagcaatgga gtattcgtca gaatggcaat cttagaagca gtgctaaatg
    30421 gaaaagcgta attataggag gatgaaaaaa tgaaaacact cattaaaaac ggtcagatca
    30481 atacacgaaa aaatatgacg acaccagcag aaatttggat agaagatggg cgaatcaaag
    30541 caatcggtac aggattctca gaagccgaat ttgatgaagt gtttgatgca aagggacaat
    30601 tgatcacacc tggtttagtg gacgtccatg ttcatttgag agagccagga tttacataca
    30661 aggagacgat cgaagctggg actcgctcag ctgctcgtgg cggtttcact actgtatgcg
    30721 cgatgccgaa tttaaatcct gtacctgata cggcagaaaa gctgagacaa gtatatgaca
    30781 tcattagaaa agatgcagtt gtgaaggtat tgcagtatgc gccaatcaca gaaaatctgc
    30841 gaagtgagaa attagttgat caagaagcac tgattgaaga aggtgctttt gctttcacaa
    30901 atgacggtgt aggtgtccaa acagctggga cgatgtatct ggctatgaaa gaagctgcaa
    30961 aaaacaataa agccctagta gcccatacag aagatgaatc tttattattt ggcggcgtta
    31021 tgcacgctgg caagaaggca gaagaacttg gtcttcccgg gatcttaagc gtaaccgagt
    31081 cttctcaaat tgctagagat ctgttgctag ctgaagcaac tggtgttcat taccatgttt
    31141 gccatgtttc tactaaagaa agtgtccgtg taatcagaga cgcaaaaaaa gcaggaatcc
    31201 acgtaacagc agaagtttca ccccatcatc ttattttgat cgacgaagat attcccgaag
    31261 atttcggctt ttggaaaatg aacccgccgc taagaggacg ggaggacaga gaagctttga
    31321 tcgaaggctt gcttgacggt acgatcgatt gcatcgctac agaccatgcc ccccatggat
    31381 tagaagaaaa aagccaaagt ttcatgaaat caccatttgg tatcgtagga agcgaaacag
    31441 cgtttcaact gatctatact cattttgtag aaactggtcg attcacgttg gaacaagtga
    31501 tcaattggtt ggcagttaaa ccggctgaga tttttggttt gaatgcagga acgctaacgg
    31561 ttggtgcccc cgctgatgta gcagtttttg atattacaca aacttgcacg atcgataaag
    31621 aagacttttt atcaaaaggg gagaatacac cttttatcgg ctggaaagta aaaggcgaaa
    31681 cacaaatgac ttttgtcaac ggaaaacttg tgtggcagaa gggagaataa aagtatggaa
    31741 cgtttgctta tattagaaga tggaacagtt ttcgaaggaa aggcttttgg tgcagatgct
    31801 agtgtcttcg gagaaatcgt ttttacaaca agtatgactg gttatcaaga aactatcact
    31861 gatcaaagtt ttaacggtca aatcattaca tttacctatc ctatggtggg taattatggg
    31921 gtaaatagag atgattatga atcgatttct ccaacatgca aaggagtagt agtaaaagaa
    31981 catgcacgtg tagcttcaaa ctggcgaaac caaatgacgc tagattcatt tttgaaacaa
    32041 aaaggaattc ctggaatatc aggtatcgat acgcgcgccc ttactcgcaa aatccgtgaa
    32101 gtcggaacga tgaaagctag tatcgttgat gctggagatt ccttcgaaca tgcatttgat
    32161 cagctgaaag caaccgttct agcaaccaat caagtgcagc aagtctcaac taacaaacct
    32221 tatccaagtc caggaacagg tcggaaagtc attgtcattg acttcggctt gaaacatagt
    32281 attttacgag aattgagtaa aagaaactgt aacctcacag tacttcccta taacacagat
    32341 gccgagacga ttttagcgct atcaccagac ggagtcatgc tgacgaatgg acctggtgat
    32401 ccaaaagatg tgccagaagc aatcgaaatg atcaaaaaaa tccaaggaaa agtaccgatt
    32461 tttggtattt gtctcggtca ccagttattt gctttagcca acggtgcaga tacctataaa
    32521 atgaaattcg gacatcgagg attaaatcac ccggtacgag aagtagcgac cggaagaatt
    32581 gattttactt ctcaaaatca tggctatgca gtcgatgaag ccacgattga tccagaaaaa
    32641 ctcatcgtaa ctcatgtaga agtaaatgac ggaacagtag aaggagtcaa acacagagat
    32701 tatcccgctt tcagtgtgca gtttcatcca gatgctgccc ctggtccgca tgatgcacta
    32761 catttatttg atgaatttat ggaaatgatg gacgcaggaa aggagcagta aacagatgcc
    32821 gaaacgtaca gatatcaaga aaattatggt gattggatct ggtccgatca tcattggtca
    32881 agccgcagaa tttgactacg caggaacaca agcctgcctt gccttaaaag aagaaggata
    32941 tgaagtggtt ttagtaaatt ctaatcctgc gacgatcatg acggataaag aagtggcaga
    33001 tcaagtatat atcgaaccga tcacacttga atttgtatca aggattcttc gaaaagagcg
    33061 tccagatgct ctccttccaa ctttaggcgg acagacaggc ctcaatatgg caatggagct
    33121 ggcagcatca ggtattttag aagaattgaa catagaactt ctaggaacaa agctttctgc
    33181 gatcgatcaa gcagaagatc gtgatctatt caaacagttg atgaaagaat tgaatcagcc
    33241 aatccccgag tctgaaatcg tcaatacagt agaagaagct gtagattttg caaataaaat
    33301 cggctatcct gtcatcgttc gtccagcctt tactttaggc ggaactggtg gaggtatgtg
    33361 tgaaaatgaa gaagaattac gtatcgtatc agaaaatgga ttaagtcttt cccccgctac
    33421 ccaatgtttg attgaacgaa gtattgcagg ttttaaagaa attgaatatg aagtgatgag
    33481 agattcggct gataatgcaa tcgttgtctg caatatggaa aattttgacc cggtgggtat
    33541 ccatacaggt gattctatcg tatttgcccc tagtcaaaca ttgtctgacc atgaatatca
    33601 aatgcttcgt gatgcttctc tttcgattat tcgagcactt aagatcgagg gaggctgtaa
    33661 tgtccaactc gctcttgatc cacatagttt taattattat gtgattgaag tcaatccgcg
    33721 tgtttctcgt tcatctgcgt tagcaagtaa ggcgactggc tatccgatcg ctaagctagc
    33781 tgctaaaata gcgcttggat taacattaga cgaaatgaaa aatcctgtta caggaacgac
    33841 ttttgcagaa tttgaacctg cattagatta tgttgtagct aaaattcctc gctggccgtt
    33901 tgataagttt gaaaacggag aacggctttt aggcacgcag atgaaagcaa ctggtgaagt
    33961 gatggctatc ggtcggaata tcgaagagtc tttattgaaa gctgtgcgtt ctttagaaat
    34021 cggtacacac catatcgaac ttcctgagtt ggcagacatc acggacgatg acctaatcca
    34081 aaaagtggtt cgagcacaag atgatcgcct gttttatgta gctgaagcta ttagaagagg
    34141 gtacaccata gaagaattag cggatttgac aaaaatcgat ttattctttt tagataaact
    34201 gttgcatatc gtagaaatcg aacagcagtt gaaacagcag ccgttggatg acaagatatt
    34261 gaaaacagcg aaacaaaacg gttttacaga caaaaaagtg gcggagctgt gggaaataaa
    34321 cgaagaagcc gttcgccaga tgagaaaaga aaatgatttg attcctgtat ataagatggt
    34381 tgacacttgt gcagcggaat tcgaatcaaa aacaccttat ttttatagtt cctatgaata
    34441 tgaaaatgaa agttcacgat cagaaaagcc ctcaattcta gtactgggat ctggtcctat
    34501 ccgtatcgga caaggggttg aatttgacta tgcgactgtg cattctgtca aagcaatcca
    34561 gcaggcagga tacgaagcaa tcatcatgaa cagcaatcct gaaacagttt cgactgactt
    34621 ttctatttcg gataaattat attttgagcc attgaccttc gaagatgtga tgaatgtgat
    34681 cgagctagaa cagccgatag gagtaatcgt tcaatttggt ggtcagacag caatcaactt
    34741 agccgaacct ttgactgctg ctggtgtaaa aatcttaggg acaacgatcg aagacttgga
    34801 tcgtgcggaa aatcgtgatt tattcgagca agcgctgaaa gagttggata ttcctcaacc
    34861 tttaggggat actgctacaa gtaaagaaga agccgtggtt atcgcttcga gaattggtta
    34921 tccagttctt gttcgtccaa gctatgtatt aggcggacgt gcgatggaaa tcgttgaaaa
    34981 ccagcgagat ttagaagatt acatggaaca tgctgtgaaa gcatctccag aacatccagt
    35041 actagtggac cgttacttgc ttggtggtga atgcgaagtg gatgcgattt gtgacggtga
    35101 aacagtactc atcccaggga tcatggagca tattgagcgt gctggtgtcc attcagggga
    35161 ttcaatggct gtgtatccac ctcaaacatt atctgaatcg atcaagaaaa cgatcgaaga
    35221 ttacacgatc cgcttagcat taggtttgaa ttgtgtcggg atgatgaata tccagtttgt
    35281 catccatgaa aatgaggttt atgtgatcga agtcaatcct cgagcaagtc ggactgttcc
    35341 attcttaagt aaggtgacag gaattccaat ggcacaaatt gcgacgaaag ctattttggg
    35401 agagaaacta aagacacttg gttatgaaaa cggactttat ccagaaacaa aagaagttca
    35461 tgttaaagca cctgtctttt cttttacgaa attgcagaaa gttgatactt acttagggcc
    35521 tgaaatgaaa tcgactggag aagtaatggg atctgacaga caattagaca aagctttgta
    35581 caaggcattc gaagcttctg gattacactt gcctgaatat ggagccgtat tatttacgat
    35641 tgcggacgaa acaaaagaag aagcactaga attagctaaa cgttttacag aaattggcta
    35701 tagtctgata ggaacgaaac atacagctgc ttatttcgaa aaaaatggtc tggtcgttga
    35761 aacagtagcg aaaatttctg aggaagcagc agaaaaaaat gtagtcgact taataagaga
    35821 tggtaaaaca caagtggttg tcaatacgat tgataaagat cggggaaatg cttctaaaga
    35881 cggtttcatc atccgaagag aagcagttga acatggtatt ccattgttta cttctttaga
    35941 cacagcagat gcgatcgttc gagtgatgga atcccgtgct ttttcaacta accctattta
    36001 acggcgagca catgttaaga caaaaagacg aaaaaccgtt gcaggagggg acagatgaaa
    36061 caagaaatga tggaaatcgt tcagcaaaaa cagctggcac caaagatttt tgaaatgaca
    36121 ttgaaaggtg atctagtaaa agaaatgcag caaccaggac agttcttgca tattctcgtg
    36181 ccgcgtgcag acttgctatt acgccgaccg attagcttga atcatatcga ccacgaaaca
    36241 aatacttgcc gtatcattta tcgggtggaa ggtgagggga ctagagtctt ctctactctt
    36301 tctgccggag accatttaga tgtcatgggt cctttaggca atggttttga tttgtccaat
    36361 cttcatacag gagacgaagt atttataatc ggtggaggaa tcggtgtccc tcctctatat
    36421 gagttatcaa aacagttagt cgaaaaagga attaagccaa cgcatttttt aggttttgcc
    36481 acacatgaag tcgtttatta tgaaaaagaa tttaatcgtc taggagaaac aagaatcgct
    36541 actgacgatg gaacatacgg tatgcacgga aatgtcggta atttattgtt aggaacgaag
    36601 acggaaccta cagctgtttt tgcatgtggt tcaaacgggt tattgaagac agtggaacag
    36661 ctgttttctt cgcatcaaaa tgtccagtta tcattagaat cccgcatggc atgtgggata
    36721 ggggcttgct atgcttgcgt ttgtcatacg ccggaagatg aaacaggaac aaaaagtgtg
    36781 aaagtttgtg acgaaggacc gatttttaaa ataggagagg taattatatg aatgagaatc
    36841 ctttagcggt caaacttcct ggcctagaat taaaaaatcc tatcatgccg gcaagcggct
    36901 gtttcggttt tggcaaagaa tacggaaaat actatgattt gaatcagctt ggatcgatca
    36961 tggtcaaggc aacgactcca aatgcccgct ttggcaatcc cacgccaaga gtggcggaaa
    37021 caccatctgg catgttgaat gcaattggtt tgcaaaatcc tggtctagac gttgttatgc
    37081 ataaacttct tccagaatta gaggagtaca atgagttacc catcatcgca aatgttgctg
    37141 gtgcttgtga agaagactat gtagaagttt gtagcaaaat tggcgaagca ccaaatgtga
    37201 aagcaatcga gctgaacatt tcttgcccaa atgtcaaaca tggcggaatt gcttttggaa
    37261 cagattctga tgttgctttt caattaacac aagcagtaaa gaaagtttct tctgttcctg
    37321 tctatgtcaa attgtcccca aatgtaacag acatcgttcc aatcgctcag gcaatcgaag
    37381 ctggaggggc agatgggttt acgatgatca atactcttct tggtatgcgg attgacctta
    37441 agacaagaaa accgatttta gccaatcaaa caggtggact ctccggacca gcaatcaagc
    37501 ctgtcgctat tcgtttgatt caccaagtag caagtatatc cagccttcca atcattggga
    37561 tgggcggcgt gcagacagtg gatgatgttc ttgagatgtt tatggctgga gcaagtgctg
    37621 tagctatagg gaccgcgaat ttcaccgatc cgtatatctg tcctaaacta attaaagagc
    37681 tccctgtgcg aatgagcgaa ctaggcatcg aatcgctgga aagattaatc aaagaagtga
    37741 gagaggaacg tgaacgatga atcaactaga ccaacgacca attattgcat tagatttttc
    37801 aactaggcaa gaggtagaag actttcttcg atttttcccg aaagaagaaa aattgtttgt
    37861 gaaaatcgga atggaattgt tctaccaaga aggacctgaa atcgtacgtt atctaaaaga
    37921 ggctggtcat aatgtatttt tagatctgaa acttcacgat attccgaata cggtcgaaaa
    37981 agcaatgcgg ggattggcaa agcttggtgt tgatctcacc tgtgttcatg ccgctggcgg
    38041 tattcgtatg atggaggcgg caatgcgagg attagaagag gggactccag aaggtggaaa
    38101 acgtccgcta cttctagcta tcactcagct tacttctaca agtgaagaag aaatgcatgc
    38161 cgatcaacta atcgaagttt ctttagaaaa aagtgtgatc cattatgcta gctgtgccaa
    38221 aaaagcagga ttagatggtg tggtttcttc tgcttgggaa gtagaagcga tcaaagaaat
    38281 ggcaggagac gaatttgttt gcttaactcc gggtataaga ccagaaggaa cagtcgcagg
    38341 agatcagaca agagtagtca caccttctca ggcaaaaaag atcggctcaa cttttatcgt
    38401 tataggtcga ccaatcactc agtcaactaa tccttataaa gcatatcaaa cgattcaaac
    38461 agaatggagc taacctaaaa tgaatgttga acaaagtatc gcaaaagact tattagaaat
    38521 tgaagcagta tttttaaatc caagtaatcc ttttacatgg gcaagtggaa tcaaaagccc
    38581 aatttactgt gataaccgaa tcacgatgag ctatccaaaa gtccgaaaag agatcgcaaa
    38641 aggattagca agcaaaatca aagaagctta ccctgaagta caagtgattg ctggaactgc
    38701 aacagcagga atccctcatg ccgcatgggt agcggaaata ctggatttgc caatggtcta
    38761 tattcgaagt aaggcgaaag atcatgggaa aggtaaccaa atcgaaggac gtatatttga
    38821 aggtcagaag atggttgtga tcgaggattt gatttctaca ggcggaagtg tcttggaagc
    38881 agctgaagca gcaaaaagag aaggtgcgga tatccttggt gttgcggcta tctttacgta
    38941 tgagttgcca aaaggaaaag caaatttcga aaaagccgaa atccctctta tgacgttgac
    39001 caattattct gtattgattg aagcagcttt agaagatcgt tatatcgatg agcaagaatt
    39061 aactctgcta aaagaatgga aaaaagatcc tgaaaattgg cagatttaag cgcgcttgat
    39121 gaagatagag aaatccgatt tgtgtctcca gaatcatcaa ttggaatagc aaaaatccaa
    39181 aaactgattg cgagaagcat cttttcatca ggtatgaaaa gaaacttcta tcgtgatcag
    39241 tttttgattt ttttaaaatt catttttcta agactggtta gcagaaagaa gagaataatc
    39301 tcttctcttt tttctattat atatagattt tctctatttt ttggtaaggg agctgtttca
    39361 aaaatggtaa agagcggaaa tgttgcgaat atcagttatt taatgatatg atattcggaa
    39421 aaagaaatct caaagggagg taacttttat ggggttgaat gaatttattt taggattgaa
    39481 caatttagag acgatcacaa gggcacctgg attttttaaa tatacagagc atacagttgc
    39541 agcacattcg tatcgtgtag catctattgc gcaagttcta ggagacattg aagaagcgaa
    39601 tggacaaccg atcgattgga aactgctcta tgaaaaatca ttgaatcatg actacacaga
    39661 gcgttttatt ggtgatatca aaacgccggt gaaatacgca agcaaagagt taagagggat
    39721 gctgcaaaca gttgaagaaa agatgacgga cgaattcatc aaccaagaga ttccagctga
    39781 gttccaagaa gtttatcgcc gtcggttgtt cgaaggaaaa gatgaaaccg tagaaggaga
    39841 actattagcc atttcagata aggttgacct tctttatgaa tcttttgatg aaatcatcaa
    39901 aaataacccg gaaaaagttt ataaggagat gttcattgag gcagtgacta cgattaaaag
    39961 ctttgaacat cggccttctg ttcagtattt ctttaaagag atatttccgg aactattgaa
    40021 ccaggaattt tatggaaaag aggcgttttt agaaaccgtc tcaacgattg taaagaaaga
    40081 agaaaaataa aaatgatatg agatgcaggg ggaagtatag ttgaaaaaga atatggatgt
    40141 cgaatggggc tattctggag acaaaggtcc ggaaaattgg gcgagtttat gtgattggtt
    40201 tgcgagaggt gcggaatttc cattgcagtc gcctattcat ttgaccaagg gggaagaaac
    40261 caaaatagaa ggagacacgc tttcctttca ttatcaaaag caacggttta ctgacaagga
    40321 attcaaaaac acgattcatt tagtaccttt tgaccaactc agttatgtag agttcaagaa
    40381 agaacgctat tatttgacag atattcattt ccacttgcct tctgaacata tcatcaacgg
    40441 aaaacaggaa gatattgaat ttcattttgt tcatatgaac agcaaaaaag agaatctagt
    40501 tgtgggtgtc atgtaccagc taatggtaca ggaaggttgg ctgtataatc aagaaaatgg
    40561 ggaaagttgg aacttagaaa aacatgagca ttgggtaaat ccggatgtgt tctttccgga
    40621 gcaaaaaagc catttccatt atattggctc attgacaact ccgcctacca gcgggccaat
    40681 ccaatggttt gtaatggatc atgtaggaaa actgaaccaa gagtttcttc tccccttcga
    40741 tggtcagtat gctcgaccga acaaccgacc aatccagccc ttgaatggaa gagaaattta
    40801 ttatttcgag gagtttgagc aatgaatatt cagcagatga aatacgtggt cgctgtagca
    40861 aacaatggta gtttcagaga agcggcaaag aaaatgtttg tctcccagcc aagtctttca
    40921 gctggtatca aagaattaga aaccgagtta gggatcacgc tgtttactcg aacgaatcga
    40981 ggggcatatt taacagaaga agggcaagac ttcttaaatc gtgccgagaa aatattggtc
    41041 cagcttgata cattagaaac tcactatcta acgagtgaaa agagagacaa tttttcgatc
    41101 gctgcccagc attatgattt cttaggtcct ttgaccgcac agctactcga ggaattcaaa
    41161 gacgagatca aacaatttcg gatcatggag acgacaacag cgagagtgat cgaagaagtc
    41221 aaagagcagc acagtgagat cggcattatt tatatgaatg agcataatca agctggtatc
    41281 agaagatatt tacaacaagg agagctaagt ggccgcagca ttggaaaatt caaaacgcat
    41341 atttttttaa ggcaaggaca tccattagct gaacacgcag agatcaccaa agaagaattg
    41401 ttatcttatc ctcaagttcg ttttacacag gatggaaata atttcccgta tttttatgaa
    41461 gaccttatcg agatccctga tcaagaaaca gtgatttata cgagtgatcg aggtactttg
    41521 atgaatatcg ttcttgaaac tgatgcttac gcttcaggat ctggtattgt gattggaggg
    41581 attcgcgagc acttgcggtt aatccctttg gcagaaggcg aactaaatga attttatatc
    41641 atttattcgg cgaaacgtcc gctcagtgaa atcgctcaaa gatttatcaa agagcttact
    41701 cggttactgg accagcagag ctaagaaaag cagatgagaa cgctgtttat tgaacaccgt
    41761 tgatcaaaaa agaggggttg tgacagaatt tt
//
LOCUS       DAZKCY010000013        40477 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000013 DAZKCY010000000
VERSION     DAZKCY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.013"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..2595
                     /gene="secA"
                     /locus_tag="ACG8XC_000615"
     CDS             61..2595
                     /gene="secA"
                     /locus_tag="ACG8XC_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP9190337.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2721..3822
                     /gene="prfB"
                     /locus_tag="ACG8XC_000616"
     CDS             join(2721..2792,2794..3822)
                     /gene="prfB"
                     /locus_tag="ACG8XC_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP9190338.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            3927..4613
                     /gene="ftsE"
                     /locus_tag="ACG8XC_000617"
     CDS             3927..4613
                     /gene="ftsE"
                     /locus_tag="ACG8XC_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP9190339.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4606..5490
                     /gene="ftsX"
                     /locus_tag="ACG8XC_000618"
     CDS             4606..5490
                     /gene="ftsX"
                     /locus_tag="ACG8XC_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP9190340.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            5642..7090
                     /gene="cls"
                     /locus_tag="ACG8XC_000619"
     CDS             5642..7090
                     /gene="cls"
                     /locus_tag="ACG8XC_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9190341.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7427..8284
                     /locus_tag="ACG8XC_000620"
     CDS             7427..8284
                     /locus_tag="ACG8XC_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP9190342.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8399..9319
                     /gene="pstC"
                     /locus_tag="ACG8XC_000621"
     CDS             8399..9319
                     /gene="pstC"
                     /locus_tag="ACG8XC_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFP9190343.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9319..10203
                     /gene="pstA"
                     /locus_tag="ACG8XC_000622"
     CDS             9319..10203
                     /gene="pstA"
                     /locus_tag="ACG8XC_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP9190344.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10218..11024
                     /gene="pstB"
                     /locus_tag="ACG8XC_000623"
     CDS             10218..11024
                     /gene="pstB"
                     /locus_tag="ACG8XC_000623"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9190345.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11040..11798
                     /gene="pstB"
                     /locus_tag="ACG8XC_000624"
     CDS             11040..11798
                     /gene="pstB"
                     /locus_tag="ACG8XC_000624"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9190346.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11811..12488
                     /gene="phoU"
                     /locus_tag="ACG8XC_000625"
     CDS             11811..12488
                     /gene="phoU"
                     /locus_tag="ACG8XC_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP9190347.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            13064..13756
                     /locus_tag="ACG8XC_000626"
     CDS             13064..13756
                     /locus_tag="ACG8XC_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP9190348.1"
                     /translation="MLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            13753..14520
                     /locus_tag="ACG8XC_000627"
     CDS             13753..14520
                     /locus_tag="ACG8XC_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP9190349.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14620..16188
                     /gene="liaX"
                     /locus_tag="ACG8XC_000628"
     CDS             14620..16188
                     /gene="liaX"
                     /locus_tag="ACG8XC_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFP9190350.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16485..16805
                     /locus_tag="ACG8XC_000629"
     CDS             16485..16805
                     /locus_tag="ACG8XC_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFP9190351.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16805..17155
                     /locus_tag="ACG8XC_000630"
     CDS             16805..17155
                     /locus_tag="ACG8XC_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9190352.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17322..18260
                     /gene="hprK"
                     /locus_tag="ACG8XC_000631"
     CDS             17322..18260
                     /gene="hprK"
                     /locus_tag="ACG8XC_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFP9190353.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18275..19105
                     /gene="lgt"
                     /locus_tag="ACG8XC_000632"
     CDS             18275..19105
                     /gene="lgt"
                     /locus_tag="ACG8XC_000632"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP9190354.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19149..20174
                     /locus_tag="ACG8XC_000633"
     CDS             19149..20174
                     /locus_tag="ACG8XC_000633"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9190355.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20189..21127
                     /gene="galU"
                     /locus_tag="ACG8XC_000634"
     CDS             20189..21127
                     /gene="galU"
                     /locus_tag="ACG8XC_000634"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9190356.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21252..21692
                     /locus_tag="ACG8XC_000635"
     CDS             21252..21692
                     /locus_tag="ACG8XC_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFP9190357.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21695..22297
                     /locus_tag="ACG8XC_000636"
     CDS             21695..22297
                     /locus_tag="ACG8XC_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP9190358.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22356..23462)
                     /locus_tag="ACG8XC_000637"
     CDS             complement(22356..23462)
                     /locus_tag="ACG8XC_000637"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFP9190359.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23672..24673
                     /gene="ccpA"
                     /locus_tag="ACG8XC_000638"
     CDS             23672..24673
                     /gene="ccpA"
                     /locus_tag="ACG8XC_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFP9190360.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25010..27355)
                     /locus_tag="ACG8XC_000639"
     CDS             complement(25010..27355)
                     /locus_tag="ACG8XC_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFP9190361.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27634..28536
                     /locus_tag="ACG8XC_000640"
     CDS             27634..28536
                     /locus_tag="ACG8XC_000640"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP9190362.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28749..30524
                     /locus_tag="ACG8XC_000641"
     CDS             28749..30524
                     /locus_tag="ACG8XC_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFP9190363.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30590..32176)
                     /locus_tag="ACG8XC_000642"
     CDS             complement(30590..32176)
                     /locus_tag="ACG8XC_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP9190364.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32427..32651
                     /locus_tag="ACG8XC_000643"
     CDS             32427..32651
                     /locus_tag="ACG8XC_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFP9190365.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32793..34544
                     /locus_tag="ACG8XC_000644"
     CDS             32793..34544
                     /locus_tag="ACG8XC_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFP9190366.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34544..36328
                     /locus_tag="ACG8XC_000645"
     CDS             34544..36328
                     /locus_tag="ACG8XC_000645"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190367.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36654..37505
                     /gene="licT"
                     /locus_tag="ACG8XC_000646"
     CDS             36654..37505
                     /gene="licT"
                     /locus_tag="ACG8XC_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFP9190368.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     EKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37598..39484
                     /locus_tag="ACG8XC_000647"
                     /pseudo
     CDS             37598..39484
                     /locus_tag="ACG8XC_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(39524..40426)
                     /locus_tag="ACG8XC_000648"
     CDS             complement(39524..40426)
                     /locus_tag="ACG8XC_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9190369.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT        13019 a         7149 c         8618 g        11691 t
ORIGIN      
        1 gaaaagtaaa gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc
       61 atggccaatt tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat
      121 agcattgcaa aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa
      181 ttacaggcaa aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa
      241 ttacttccgg aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt aggtttatat
      301 ccataccacg tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg
      361 cgtactggtg aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact
      421 ggcgaagggg tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa
      481 atgggcgaac tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct
      541 tcggatgaaa aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta
      601 ggatttgact acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg
      661 ctgaattatg ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg
      721 ctgatcattt cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc
      781 gtgaaacgtt tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt
      841 ttaaccgaag cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac
      901 attgaaaata ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg
      961 cttttagata tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact
     1021 ggtcggatca tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa
     1081 gaaggcgtag agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat
     1141 ttccgtatgt acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa
     1201 ttcagagaaa tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt
     1261 gatgatcgtc cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa
     1321 gatatcaaag atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa
     1381 acatctgagt tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat
     1441 gcgaaaaatc actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg
     1501 acgattgcta ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga
     1561 gaacttggtg gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac
     1621 cagcttcgag gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca
     1681 ttggaagatg atttgatgaa acgattcggt tcagaacgaa tcaaagcttt cttagatcgg
     1741 atgaagatcg gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa acaagtagaa
     1801 tctgcacaaa aacgtgtaga aggaaataac tatgacacac gtaaaaacgt cttacaatac
     1861 gatgatgtga tgcgtgaaca acgtgaagta atctataaac agcgacaaga agtaatcatg
     1921 gaagaaaaaa gcttatccaa gccactaatg aacatggtca aacggacaat cagtcgtgta
     1981 gtagatgcgc atacacaatt agagaaagaa aattggaatt tggaaagtat catcgatttt
     2041 gccggcagta ctcttgtcca tgaagatagt attactcttg cagatatcga aggaaaaaca
     2101 gctcaggaga tcaaagagta tctaaacgaa cgagcacaag aagtatttaa taccaaagcg
     2161 gctcaactaa atggtccaga gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt
     2221 gtggactcta aatggacaga ccatattgac gcaatggatc aattacgtca gtcaatcggc
     2281 ttacgtgctt atggacagaa caatccatta gtcgaatatc aaacagaagg ctataagatg
     2341 tttgaagata tggtaggcgc catcgagtat gaagtcactc gaatctttat gaaagctgag
     2401 atccgtcaaa acgtacagcg tgaacaggtt tctcaaggaa cagcttctca ctcagaagac
     2461 ggagatgcca aagaaaatag taatactagc gcgaaaaaac agccaatcca tgtgaacaag
     2521 gtcggaagaa atgatccttg tccttgtgga agcgggaaaa aatataagaa ctgtcatgga
     2581 aaaaatgcaa actaatttct gacagcagga ttggtgagac aaattctttt tttaagaatt
     2641 cgtctcacct tctttctgtt atttgctgat tctgttagac tagatataat gaaaaaagta
     2701 tagtgaaaga aggaaaaaac atggaaatat ccgaaattcg aaatctcctg acagaaatgc
     2761 aggaaaaggt cacaagtttc aggaggtctc tttgacttag atcagctgga agaagacatc
     2821 gcggaagcag aagcaaaaat ggctgaacct ggtttttggg atgacagcga agccgcacaa
     2881 aaagtgatca gtgaaaataa cgctaataaa gaaacctatg accaattcca ttctcttgcc
     2941 gaagaactag aagaaatcga attgatggtg gagatgctgc aagaagaacc tgatgcagca
     3001 atgcaagaag aagcagaaac acgtatcaat gcattagatg aaaaaatgaa gacgtatgaa
     3061 ttatctatgc tgttagacgg accttatgat cgaaacaatg cgatcgtcga attgcatcca
     3121 ggggctggcg gaacggagtc ccaagactgg ggcagcatgc ttttacgtat gtatacacgt
     3181 tgggcagagc agcatggcta tcaagtagaa acactagatt atcaagcagg tgatgaagca
     3241 gggatcaaga gtgtgaccct gctgattaaa ggacataacg cttttggtta tctaaaatca
     3301 gaaaaaggtg ttcatcgcct tgtacggatt tcgccttttg actcagcgaa acgacgtcat
     3361 acttcttttt gttcggtaga tgtgatgcca gagcttgatg aaacgatcga tatagaaatc
     3421 aatccagatg atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc
     3481 aataaaacag aatctgctgt acggatcact cacttgccta caggaacagt agtagctagt
     3541 caggcacaac gctcacaatt gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag
     3601 ctttatcaat tagaagtgga aaagaaagaa caagaagcag ctgcacttcg aggagaacaa
     3661 aaagaaatcg gctggggttc acagatccgt tcttatgttt tccatcccta ttctatggta
     3721 aaagaccatc ggacaaacgt agagacagga aatgtccaag cagttatgga cggtgacctt
     3781 gatttattta ttgacggcta tttaaaaatg catctacaat aagaacccat tgttaaattg
     3841 aaataaaatt gtaagatttt gcaagcatgt tgaaacattg ccatgctata atagctaaag
     3901 actgaaaaag atatggagag aacgccatga ttgaaatgaa ggatgtaatg aagaagtact
     3961 caaatggtac gaccgctatc cgcaatctct cgattgtcat cgaacaagga gagtttgtct
     4021 atgtggttgg tccgtctgga gccggtaaat ctacattcat caaattgatg tatcgagaag
     4081 aaaaagcttc aaaaggaact ttgaacgtcg caggtcatga tctaatagca atcaaaaacc
     4141 gcgaagttcc ttatctacgc agagaaatcg gcgtggtgtt ccaagactat aaattattac
     4201 caagaaaaac agtatatgaa aatgttgcgt acgctatgca agttatcggc agaaaacctc
     4261 gagatatcaa aaaacgagta atggaagtgc tggaccttgt tggtttgaaa cacaaagttc
     4321 gagtctttcc tagcgaatta tccggtgggg aacagcaacg tgtctctatt gctcgggcaa
     4381 tcgtgaatac gcctaaagta cttatcgcgg atgaaccgac aggaaatctg gatccagaaa
     4441 actcatggga aatcatgaaa ttattagatc ggatcaatgc ccaaggaact actgtcgtga
     4501 tggctacaca taacagtacg atcgtaaata cgattcggca ccgagtaatt gctatcgaaa
     4561 acggccgcat tattcgagat caagcggaag gggaatatgg atacgatgat tagaaccttt
     4621 ttccgccatt tattagaaag tatcaagagt ttgaaacgta acggctggat gacagttgct
     4681 tcagtcagtg cggtaacgat cacgttgact ttagtcggtg tattcatggc agttatcatg
     4741 aatgcaacta aattagctca ggatatagaa ggaaacgttg atgtatctgt attcgtagat
     4801 atcggtacaa aagacgaaga gatgaaaaag ctagaaaaag aattgaatga cttagatcat
     4861 gttaaaaaag ttgaattttc aagtaaagaa caggaattga aaaaaatcca aaacgaaatg
     4921 ggtgattcct ggaaattgtt tgaaggtgac aataacccgt tatatgatgt gtatgtagta
     4981 agtgctactg atccttctta tacaaaacaa gtagcagaag aagcagcaga tttgaagaat
     5041 gtttctcgtt ctgattacgg tggagcttcc tcagatcgga ttttccaaat cgctggtgca
     5101 gttcggacat ggggattagt tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt
     5161 tcgaacacga ttcggatcac gattttgtct agacagcgtg aaattcaaat catgcgttta
     5221 gtcggtgcaa aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt
     5281 ctgatcggtg cgattcttcc gattttgatc atcacatttg gttatgcatg gtttttccaa
     5341 ttgatcaatc catcattatt acgttcccat tattcattga ttcatccaca aaatatcgtt
     5401 tggaaaatca atcttttaat ggtcggcatt ggtgtgatca ttggttcact tggctcgatc
     5461 atttctatga gacgtttctt gaaattctaa taccacaata taaataaaat gagaaagttg
     5521 gacagtttcg ttccagcttt ctttttttat tggctaggag aatagaagga agcggctttt
     5581 ttccctttac tttaaaaaac aacttgggta ttcttaagtt ggtagaggaa gaggtgaagc
     5641 cgtgaaaatt atcgttgaca atttttttac gatccttttg attatgaata ttttgctttc
     5701 ctttatcatc gtttttcgag aaagaaagga aactgctcaa acatgggcat ggctgctcgt
     5761 tttgatgttc attccagttg taggatttat tttatatatt tttttaggaa gagggatttc
     5821 caaagagaaa atctttgatt tgaagatcca agggaaaatc ggtaagaacc tggaaattga
     5881 agaagacaaa caggcattga tgcgagggct ttatcctcat ccgcctactg gacaagtaga
     5941 tgtgaaacaa ttgatctata tgctcacggt atttgaaagt acgctctata cgactaaaaa
     6001 tgaaatcact ttatttacag atggtcgtga aaaattcgat gcgctgattg aagatatcaa
     6061 gcaagcagaa gaccatatcc acttccagta ttatatttat cgcagtgatg cattaggtga
     6121 agaagttcga gatgcattga cagaggcggc aagacgaggg gtaaaagtca gagtccttct
     6181 tgatgcatgg ggttccacac aagtttcttc cagctttttt cagaatttga aaaaagcagg
     6241 cggagaaatc gcatttttct ttcctctatt tgttccgtat attaatcctc ggatcaatta
     6301 tcgcaatcat cgtaaaatcg tagttattga tgggaaaatc ggatatacag gtgggttcaa
     6361 tgttggaaat gaatatctag gattggtaaa gaaatttggt tattggcgag acaatcattt
     6421 gcgtatttac ggagaagcgg tgtatatctt gcaaaaccgt tttttgatgg actggaattc
     6481 tcagcataca aaagaagtag gctattctcc caaatttttt ccgtccattc actctactgg
     6541 tgatatagct gttcaaatcg tgacaagcgg accagatacc gaacatgaac aaatcaaaat
     6601 gacttatttg aaaatgattt cgttagccaa acgggagata ttgatccaaa cgccttatta
     6661 cataccggat ggatcgattc atgaagcact gaagttggct cttctttcag gcgtaaaagt
     6721 tcatatccaa atacctaaca aaccagatca tcttctcgtt tactgggcaa cgtattcttt
     6781 cgctgcagag ctgatcgagt atggagcaag gatcgaaaca tatgagaatg gatttattca
     6841 tgcaaagacg atgattatcg atggtggcat tgtttcagtt ggctcagcaa atatcgatgt
     6901 tcgttcattt cgcttggact ttgaggtcaa tacactgatt tatgatgaac gaatggcttc
     6961 acgtgtaaga aaagcatttt ttgaagactc gaaaatttct acgcatctca caaaagaaat
     7021 gtatgaaaac cgaggaataa tcatcaagat gaaagaagga ttagcaagat tgatttcgcc
     7081 gctcctttaa aacacaaaaa ggacatgaga accatcaaca gatggagatc tcgtgtcctt
     7141 ttctaacggg gccatttctg agagtacgtc agaaatggag aaagcgtctc gcatgagcga
     7201 aattaacttt ggggtatctc ttgttttttg gggaaagcaa gaaataagct aatatatgct
     7261 attaaaagag acacatttca aagccagtat agcatagaaa cttttaatga gatagttttt
     7321 tttacaatta tgaggattta ttttgttatt taatgatttt gattcgtttt catcaagtaa
     7381 tgaaaatgat aaaataaaca tactttagga ttaaaggaga tttctaatga aaaaacttaa
     7441 attattctta ccaataatag gattagctat tcttttatca ggctgttcaa agtggattga
     7501 tcgaggagaa tccattacgg ctgttggctc ttctgcttta cagcctttag tagaaacagt
     7561 tgctgaaact taccagacag aaaatcctgg aaaattcatc aatgtacaag ggggcggaag
     7621 cggaactggt ttgtctcaag tacagtcagg agcagtcgac atcggaaact ctgatttgtt
     7681 tgcggaagaa aaatcgggga tcgatgcctc agcattagtc gatcatcgag tagcagttgt
     7741 tgggatcact cctattgtga acaaggatat tggcgtaaaa gacatcagta tggaaaacct
     7801 gaaaaaacta tttacaggcg agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc
     7861 agtagttata ttgaaccgtg cttcaggaag cggaacccgt agcacgtttg aaaaatgggt
     7921 attggacgga gaaacagctg ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca
     7981 gatcgttgcc gacacacccg gtgcaatcag ctatgttgct ttttcttatg tttccgatga
     8041 tgtagatacg ttgaatgtta acggtgtaga accgacagat gaaaatgtga caacgaacaa
     8101 atggacgatt tgggcatatg agcatatgta cacaagagat catccaaaaa aactgacaaa
     8161 agaattttta gactatatct tatctgatga agtacaagat aacatcgttg gagaactagg
     8221 ttatatccct gtttctcata tgaaagtcga acgtgattgg caaggaaata tcgttaaaga
     8281 gtaatcttta cacaaaattt acaaacttag ctaaactttt acatgttatt aaaaccaaat
     8341 tgatgttcaa tttgtacact gagtgagtat gttaaacctg attaaggggg agtcatctgt
     8401 ggaagatgtg aaaaaagtat tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg
     8461 aaaattcatc agttttttat gtatcgcgct gatcgtgttg gttgtattat ctatttttta
     8521 ttttgtagct agtaaaggat tggccacctt ttttactgat aagatcaatg tatttgattt
     8581 tctgtttggt acagattgga atccaagcca aatcggagca aatgggaaac caatggtagg
     8641 ggcattgcct atgatcacag gttcgtttat tgtgaccttt ttatctgcta ttgttgccac
     8701 accatttgcc attggtgcag cagtattcat gacggagatc tctcctaaaa acggaacgaa
     8761 gatcttacag ccggtcatcg agctgcttgt aggtattcct tctgtcgttt atggttttat
     8821 cggtttatca gttatcgtgc cttttgtcag aagtattttt ggcgggacag ggtttggtat
     8881 cttagctggt acatttgtgt tgttcgttat gatcttgccg actgtcacaa cgatgactgt
     8941 tgatgcatta aaggcagtac cacggcacta tcgggaagct tcattagcgc ttggtgccac
     9001 aagatggcaa acaatctaca aagtcgtgct acgagcagcc gtgcccggga ttttaacagc
     9061 agtcgttttt ggtatggctc gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg
     9121 taatgcagca ttgatgccaa caggacttac tactcctgct tctacattga cgtccatctt
     9181 aacaatggga atcggaaata caatcatggg aacagttgaa aataatgtgt tatggtcatt
     9241 agcgctgatc ttgctattga tgtcattatt ctttaatatc gtgatccgta tgattgggaa
     9301 gaaaggagag ctgaaataat gaacgcaaaa cgctcagata aaattgctac aggtgtttta
     9361 tatgtagttt cggggatcat cgtattgatt ttagcagctt tactacttta tatcttagtt
     9421 cggggaatac cgcacatttc atggtccttc ttgaccgaac cttccaaagc ctaccaagaa
     9481 ggcggtggaa tcgggatcca gctatttaac tcgatttatt tgcttttgat cacgatgatc
     9541 atcagttttc ctatttcttt aggtgcgggt atctatcttt cagaatatgc tggaaaaaac
     9601 tggctgacag atgtgattcg cacgtcgatt gaaatcttga gctcattgcc ttctgtcgtt
     9661 gtcggtttat tcggtttctt ggttttcgtt attcaattcc agtatggttt ctcgattatt
     9721 tctggtgcat tggctttgac tgtattcaac ttgcctcttt tgactagaac agttgaggag
     9781 tctttacaag cggtccatta cacacaacga gaagcaggat tggctttagg tttgtcacgc
     9841 tgggaaacag tgataaaagt ggtggtttca gaagcgacac cagggatttt gactggtgtg
     9901 atcctaagtt ctggaagaat ttttggtgaa gcagctgcat tgatctatac ggctggacaa
     9961 agtgcacctg cgttagattt tacgaactgg aatccattaa gtgtttcaag cccgatcagc
    10021 attttccgtc aagctgaaac attagctgtc catatttgga agatcaatac agaaggcacg
    10081 atgcctgatg gtgtttctgt ttcggctggc gcttctgccg tgttgatcat tgctgtttta
    10141 ctgttcaact tcggcgctcg tgcgattggc aaacgattat accgaaaaat gacttcagcc
    10201 taaggagaag aagcaaaatg aaaaaatata actggaatga aacaaatgtc atcaccatgc
    10261 caaaagagaa agaagtagca ctatatacgg atgatctaca tgtctggtat ggcgacaatg
    10321 aagcaatcaa aggagtggat ctggaatttg aaaaaaataa gatcactgca ctgattggtc
    10381 cttccggctg cggaaaatct acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg
    10441 ctaatacgaa aatcactggg aaaatcatgt atcaaggcgt agatattaat gcgcctcaag
    10501 tggatgttta tgagatgcgc aaacggattg gcatggtttt tcaacgtcct aatccgttca
    10561 gcaagtcgat ttatgacaat attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa
    10621 agaaattaga tgaaatcgtt gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa
    10681 aagacagctt ggacaaaagt gccttggctt tatctggcgg gcaacagcaa cgtttatgta
    10741 ttgctcgtgc gatcgcgatg aaacctgata tcttgctgct agacgagccg gcaagtgctt
    10801 tggaccctat ttcgacagga acggttgaag aaacacttgt aaacttgaaa gaagactata
    10861 cgatcatcat cgtgacgcat aacatgcaac aagcggctcg tatcagcgac tatactgcct
    10921 ttttctatct aggaaaagta atggaatatg acaaaactaa caagatattt accagaccaa
    10981 aaattcaggc aaccgaagat tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca
    11041 tgacaaaaga aattatttca tctaaagatc ttcatctgta ttatggaaag aaagaagcac
    11101 taaaagggat cgatctaagt ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg
    11161 gttgcgggaa atccacttat ctgcgttctt tgaaccggat gaatgacctg attccgaatg
    11221 taacgatcac cgggagtgtt ttatataaag acaaagacat ctatggtcca aaaacagatg
    11281 ttgttgaact aagaaaagaa atcggcatgg tattccagca gccaaaccca tttccttttt
    11341 ctatctatga aaatgtcatc tatggattga aattaaaagg ggaaaaaaat aaaaaagtcc
    11401 ttgatcaagt agttgaagaa agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca
    11461 aattgcataa aagtgcttta tctttatccg gtgggcagca gcagcgtgta tgtatcgcac
    11521 gtgttttggc agttaatccg gaaatcatct tattggatga accaacaagt gcgcttgatc
    11581 ctgtttctac tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg tatacgatga
    11641 tcatcgtgac ccataatatg tctcaagctt cacgtatttc agataagacg gcattctttt
    11701 tagatggtca cttgatcgaa tacaatgata cgaagaagat attcatgaat ccagaaaaac
    11761 aagagacaga agattatatt tcaggtcgat ttggataagg aggaaacgga atgctacgta
    11821 cacaatttga agaagaattg ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg
    11881 tgagcagcgc cgtgcacaag tcagttcgtg catacatcaa acacgataaa atcttagccc
    11941 aagaagttat cgaaaacgat ataaatatca ataacatgga aacacgcttg gagaagaaaa
    12001 gttttgaaat gatcgcattg caacagccag ttacaactga tttgcgtatg atcattacgg
    12061 tcatgaaagc tagctcggat ttagaacgta tggccgatca tgcggtgtct gttgctaaat
    12121 ccacgattcg cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag atctccgata
    12181 tgtccgatta tgtgaaaaaa atggtggaca acgtcctagt tgcttatgtc aaaacagacg
    12241 aaaaagacgc acgtacgatc gccaatatgg atcaaagagt caatgactat ttcaatcgaa
    12301 tctataatgc aacaatcaaa aatatgcagg aaaatcctga gacagtcatc agtggaacag
    12361 actacttgaa tgttgccagt tatctagaac ggatcggaga ctatgtgaca aacatctgtg
    12421 aatggatcgt ctacttggct acagggaaaa tcactgagtt aaatacgaac cacaatgaga
    12481 ttttctaaac taaagcggtg attctgccgt ttgtttagga aaagaggttg tgaaatcagc
    12541 ggttgtgaaa aagctgttct gcatcaagta ataagaacag tcaaacaaaa atagctcctc
    12601 atatttttca tttggctgga cttattaccg cagatgcaag cttttgaaca ctgtttattc
    12661 gggaaagggt ttgtgacaaa atatgtcaca aacccttttc atcatttcat agaaaggata
    12721 aagaatgcaa aaaaaaatta gcatcatcac tatatttttt atttgtgtgc ttacttcatg
    12781 ggttattcag catcaagctg ctttatatga agtacctgtc atcgagatcg aaaaagtacg
    12841 aataaaagag gatcatcaat tgattacagg aagactgatg aacacgaatc aaaaaggaga
    12901 atcgatttat ttatcggttc cttatcaaaa aaatcaaata gaaacagcaa atataaaagc
    12961 gagacaaatg tatctagtcc aaatgcatgg agatgaatgg cagctagtag caaaaaaaaa
    13021 tgatggctgg cttttcttct tcacaagtat cttcattggc gccatgttgc ttattggcgg
    13081 aaaacatggt ctatttgctt taggaagtct catcatcaat atcgttttac ttttactttt
    13141 gttgctcatc tttactaaaa tagaagggat ttctctatta aatatatcta tcctgttcgt
    13201 cgttataggg atgattatta gcatattggc tttagatgga tggaatagaa gcagtataat
    13261 caaaatttca gctgcagtta cgagtgtttt tttagcattt tttatttgtc ttttgacaat
    13321 gaatctttgg aaagaccaag gactgcgcta tgaggaactg aattatctga cacgaccgta
    13381 tcgttctgtt tttctttcta gcgtactgat tggaacagct ggaggaacac tcgataccgt
    13441 tatcacagtc attgcgacat tagaagaatt gactaagcga caaccagaaa taacagcgaa
    13501 acagttatgg ctttcgggaa ggcaagtggg tcgagatgtg attggctcga tgaccaatat
    13561 ccttttgttc gtctatatca gtgggtcagt tccatcgctt cttttatatt taggaaatca
    13621 atggtcgttc aaagaaacga tagaacagca tttatcattg gaatttttac gcgtcattgc
    13681 tggcagctta ggaatagtat tatcgatccc tatctcggtc ctcttttttc tgactgtaag
    13741 gaggctgaaa cgatgaatgt acttattata cttgttggga ttttagcaat cagcgtgcta
    13801 cttattggcg gtacgcaagg tatgtatatt cttttaggct tattcatcaa tttagggatc
    13861 tttttcttgt tgttattcgg ttatcatcag aagtggccga tactggcatt gtcggtcata
    13921 ggatttttat tgattgctgt ggttatctta ttttttatca acggttataa tctaaaaatg
    13981 cgtgcagctt ttgtgagtat actgatcttt ttattttgtt tcctactatt gcttcctatc
    14041 actgattttt tagcaatcca aggttttaca acgatagaac tagaagaatt atctggctta
    14101 gataagactt tagcgattga ttttagatta cttacgagat cgttgcttct gattagtctt
    14161 agtggtgctg ttttagatgc gagtgtagca atttctagtg ggacattcga agtatatcag
    14221 gcaaatccgc aattatcttt ttaccaattg cgccattcta gtttcgctat tgctaaaaaa
    14281 atattggcgt caacagtaat gacacttctt tttgcattca tgggaagttc tttagcgttg
    14341 atcctctggt ttattgattt agacattcct tttcagcaag tgatcaatga aaaatcattt
    14401 gttctagaat atacgatggc tattcttacc acattgtccg cactacttgt tcttccttta
    14461 acctgcgtga ctacagcata tctctttaca aaaaagaaag aacaactcaa gatggagtaa
    14521 aagttacaac ttaagtccta tgacattttt actgaatcag tgcataatat ggatgtaagg
    14581 aaaagcttgc aaacaatttt gcttaaggag gcaatttcca tgaaagaaag agaacgtatt
    14641 ttagatttag tgaaaaaagg aatcctttcg actgaagaag gcttagattt attagaaagc
    14701 atggcaactg aaaaagacga aaagcaaatc aaaaaagaag cagacaaggt aactgctagt
    14761 cataaagaaa aagatcaggc aagtcaattg atcgataaac ttgaaaacgg cgaagaagaa
    14821 atttctgagc cagttgatcc aaaggaaaga gaaagacaag atcaagaaaa cttagaaaag
    14881 atcttagatg aactagcaac tgaagcgaat aaaacttcgg cgcgtctaga tgaagtaaat
    14941 gctcaattcg ctgacaataa ggcagcacgc aatgagaaac aggaagcatt gatgcagctg
    15001 aatacaaaag aagaattagg cgagttgaca gaagaagaat tagcacagcg tcagacgtta
    15061 gaagcagaaa tcaaagagtt agaagctagt ggcgatacac tgttagaaga acaattgaaa
    15121 ttagaagcag aattaaaaga tatccgtaag aatcaatggt ctgagaaaaa agaaacgttt
    15181 actgataagt ttgaattgcc agatgattgg aaagatcaag caacagatac actgaatcaa
    15241 gtaggcgaaa aaatgtcaga agctggatca cagttaggaa aattcttgaa gaaaacattc
    15301 caaactgttt cagaaacagt gaatgacaac atggaatgga aagatgtcag cttgcgtgtt
    15361 cctgggattg cgacaacgaa atttgagcat gaattttact atgaagctcc tgcagcaagt
    15421 atccttgata tcaaggcagc aaacggaaac gtgacgttaa aaacgtggga cagcaacgat
    15481 gtgaaagttg aagctaagat caaactttac ggaaaaatgg gtgctgaacc atttgaagca
    15541 ttctctgaac ggagccagat tgaggttaat gaagatcata tctccttcca aattccaaat
    15601 aaacgtgtgc gtgcagacct tgttttctac ttacctaaac gagtatacga ccacgcagct
    15661 attaaacttc taaatggcaa catcatgatt gaaacattgg aagctaaaga tatttataca
    15721 aaatctacga atggtaacat catcgtggac caattaactg caacgatgct ggaagtagaa
    15781 ggtgtaaacg gcaatatcga tattcgaaac gggaacatct tagattctat tatcgaaaca
    15841 gtaaatggta cagtgacatt tggtgcgaca gcagaaaacc tttctgtgtc tttggtaaat
    15901 ggtgatgtac gtcttacgat taaagaagat aatttgaaaa aagtagaagc aagttcagtg
    15961 aacggaaatg tcaaagtcgc tttaccagat ggcatcggtt tggaaggaca tgctaaaaca
    16021 agtcttggaa gtatcaacag ccgtctgtcc aattacgaag tagttcgtga gaaaaaagaa
    16081 cggacaaacc aaatgttgca attccgtcgt gtttcagatg gtgaaatcgc gcaagtgcaa
    16141 ctttcaacga caacaggaag tatctattta aaagatacag ataaataaag aggttgtgaa
    16201 agagatgttc agccttgagc aataagaagc gattttgtaa aatagcttcc cctattttac
    16261 aaaattgtgc cttattgccg ataggctatc tcttgaacac cgtttaccaa gagattatga
    16321 acttgccaat tatcgccgag tcacaaggaa taatttcaaa aaaagctcgt cacgttttat
    16381 gaatatggta atttgtgacc gagaattttg caaaaagaca ccgattaaga agaaggttgt
    16441 cacaacgatt ttcttcattt aaaatagtag aacagaggtg agacatgatg aaaaaaaagt
    16501 tgacaaaatc caataaaaat gttgttttga cagggacttt agcaggaata ggagaataca
    16561 tcgggattga tccgactgtt atccgtgtgc tttatgtatt cctgagctta gttgcttttg
    16621 gaagtccaat cattttatac attttattag ctttgattat cccatctgcg cgttctgcac
    16681 gaacaaacta tggacataac aatgcatatt atgaaaaaaa caattatcgt gaacaaactc
    16741 gtgaacaaac aaaaaaacgc aaagaagcgg aaaaaatcga tgatgacgaa tggagtgact
    16801 tttaatgtcg tactttcaac gtattttagt taatacgtta acattcatat cattggctgt
    16861 tctttttcct gcgatggtcc atgtgagcag tatttggacg gccgttcttg ccagcttcgt
    16921 attgtctgtg ttgaatatgt ttgtcaaacc gattttgacg atactgtccc tgccctttac
    16981 attagtcaca ttgggattgt ttacgtttgt tatcaatgca gcgattctta aaatgacctc
    17041 cttttttgtg ggagcagaac attttagttt ttcttcattc ggtgcagcgt tgttagtagc
    17101 tatcttgatg tcgcttgtca atgtaattgt cagcgaacat aacagtagaa aataaaagaa
    17161 agctgtgccg aactatcggc gcagtttttt tatttagaat caggaatgcg ttttctatta
    17221 cattggtaat taaataaaaa cgtttttttt aattggtagt acttttggct agacaatgat
    17281 aaaatgatag agaacactct aatagaaatg aggtagcatg aatgtcagaa gttgtaaaag
    17341 ttagccaatt agtgaaaaaa ttatctttgg aaatcgtgac aggcgacgag aaaagcttag
    17401 accgtgtcat cgtaactggg gacatctcac gcccaggact agagttgact ggttatttta
    17461 attattattc ccataatcgt ttacagctat ttggcagtaa agaaatcagt tttgcggaac
    17521 ggatgatccc agaagagcgt ttgatcatca tgagaagatt atgtagtgaa gatatgcccg
    17581 catttattat ttcaagagga ttaccagctc cagaagaaat gatccaggca gcaaatgaaa
    17641 atgggatagc tgtactgcgt tctcccatct caacttctcg cttattagga gaattatcca
    17701 gttatttaga tggtcgcctt gcccctcgaa caagtgtcca cggtgtttta gtggatgttt
    17761 acggattagg tgtactgatt caaggtgaca gtgggatagg aaaaagtgaa acagcgttgg
    17821 agttgatcaa acgcggtcat cgattgatcg cagacgatcg agtagatgtt tatcagcaag
    17881 atgaattaac ggtgatcgga gaaccgccta aaatcctgga acatctgatt gaaatccgag
    17941 gaatcgggat cattgatgta atgaatcttt ttggtgcgag tgcggtacgt ggctctatgc
    18001 aagtccagtt ggctgtttat ctggaagcat gggcaaaaga taagaaatac gaccgtctgg
    18061 gctctgaaga cacgaccgtt gaaatcgcgg aagtaggcat tccacaaata aaaatccctg
    18121 tcaaaacagg acgaaacgtt gcaatcatca ttgaagtagc cgcgatgaat ttccgtgcac
    18181 gtacaatggg ctttgacgca acgaaaacat ttgaagaaag attaagtcgc ttgattgaag
    18241 aaaactcagg caacgactag caaggagtga caatatgtta gcacaaatca atcgagttgc
    18301 ttttgagctt gtagggattc ctatctactg gtatgcattg atcatcgtat caggtatcat
    18361 tatcgccatg tggcttagca gcagagaggc tgttcgggta ggattaaaag cagatgatgt
    18421 gacagatttt atgttgtggg gattgcctat ctccattatt ggcgctcgct tatattatgt
    18481 tttatttgat cttccccagt atatagctga tccaatccaa atattcaata tccgttcagg
    18541 aggcttagct atttatggcg gactgattgc aggcgcgatc acgctgtatt tttttactaa
    18601 atatcatttt atttctttgt ggactttttt agatatcgct gcaccaagtg tattattagc
    18661 acaagcaatc ggtcgttggg gaaatttcat gaaccacgaa gcatatggac cagacacgac
    18721 gcgaagcttc ttagaaagtc tgcatttacc gcaattcatc attgataata tgtatatcga
    18781 agggatctat cgtcaaccaa catttcttta tgaatcactt tggagtttga ttggatttat
    18841 tttgcttttg tttttacgaa agaaaccaca ccttttgaaa aaaggtgaag tccttttatg
    18901 ttatctaatg tggtatagtt tcgggcgttt ctttattgaa ggattacgta cagacagtct
    18961 ttacttttta ggtgtgatcc gtatttctca attattatct gctgtgttat ttgtcggtac
    19021 attgattcta tttatttggc gcagaaaaaa aggaaattta ccggattatg accgctcatt
    19081 aggaaaaaat caacaaatta tctgattttt agagcgttaa aaatgaaaaa aatttcagga
    19141 gtggtaaaat gaattataga gtagcggttt taggaccagg ctcttggggt actgctttgg
    19201 cacaagtcct cgccgaaaac ggacatgacg ttcggatttg gggacatcgt gcttcacaag
    19261 tagaagaaat caacacccaa cacacgaatc aacgttattt accagatcat tgtctacccg
    19321 attccatcca agcattcacc aatatggaag atgcagtaaa agacgcagat gcggtgctat
    19381 tcgtcattcc aacgaaagca atacgttctg ttgcaaaaga actagtgcct aaattgaata
    19441 caaagccagt gatcatccat gcaagtaaag gattagaaca ggggacacat aaaaggatct
    19501 ctgatatctt gttagaggaa atcccagtag aaaaaagaaa agctgttgtt gttctttctg
    19561 gaccaagcca tgcagaagaa gtagcgaaac atgacatcac tacgatcaca gctgcaagta
    19621 cagatgcaaa agcagcaaaa tatgtacaag agctgttcat gaataattat tttcggatct
    19681 ataccaatcc tgatgtgatc ggtgtagaga tgggggcagc tttgaaaaat attattgcta
    19741 ttggtgctgg cgctattcac ggcttagggt ttggcgataa tgcaaaggca gcaatcatga
    19801 cgcgcggatt agctgagatc agccgtttag gtgtagcaat gggtgccaac ccgctgacct
    19861 ttatcggatt aagcggagta ggcgatctag tcgttacttg cacaagtgtt cattctagga
    19921 actggcgcgc aggcaacttg ttaggacaag ggcagcccct agaagaggtg ctagccaata
    19981 tgggaatggt cgttgaaggt gtgaatacta cccaagcagc gatggaacta gcccaaataa
    20041 tggaaatcga aatgcccatc actcaaacaa tctatgaagt gctgtatgaa caaaaagaaa
    20101 tcaaaacagc cgcaaaagaa atcatgttac gtgatgggaa aaaagaaaat gaattctcat
    20161 taacaatttt ttgaaggagc gaatgtatat gaaagtcaaa aaagcagtta ttccagcagc
    20221 aggattagga acaagatttt taccagcaac aaaagctatg gcaaaggaaa tgttgccaat
    20281 tgttgataaa ccaacaatcc agttcatcgt tgaagaagcg ttgaaatcag gtatcgaaga
    20341 catcttgatc gttacaggaa aagctaaacg accaatcgaa gatcactttg attcaaatat
    20401 tgaattagaa acaaacctaa gtgaaaaagg aaagaccgaa ttattgaaat tagtagaaga
    20461 aacgacagat gtcaacttgc atttcatccg tcaatctcat ccaaagggtt taggacatgc
    20521 cgttcttcaa gcaagagcct ttattggaaa tgaaccattt gtcgttatgc taggcgatga
    20581 tataatggaa gacgaagtac cattgacaca gcaattaatc gatgattacg aacgtactca
    20641 tgcttctacg atcgccgtta tgcaagttcc tcatgatgaa acttcaaaat acggaattat
    20701 caatccagga gaagtattgg aagatgggtt atacaatgtc aaaaactttg ttgaaaaacc
    20761 agatccaagc aaagcaccaa gtgatttagc tattattgga cgttacttat tgactccaga
    20821 aatttttgat gtattgtccg agcaacaacc aggagccgga aatgaaatcc aactaactga
    20881 cgcaatcgat acattgaata aaacacaacg cgtatttgcc cgtaaattca caggtaaacg
    20941 ttacgatgtc ggtgacaaat ttggttttat gaaaacaagt attgaatatg gtctaacaca
    21001 tccagaagta ggtgcacctt tacgtgagta catcctgtct ttaggtaaaa cattagaagc
    21061 agaaggaagc aagatcgatc ataatccaaa aggaataaca gaaatcaaag aagaaaagaa
    21121 aaaataaccc ttttttctaa cgagcttcag ctgctaattg aataaaaagt agttgaagct
    21181 cgtttttatt tgtttaaaaa acacgaaaat gctatcctta tagaaaagag agacaagaag
    21241 ggagtttgat catgtcagga ggagaaattg caggattgat cgcagcaatc gcctttgcga
    21301 tacttgtcgt ttttatcgtt cgtgtgttgt tgcaagtttc taaaacttta gaaaaagtag
    21361 atcagactgt agcagaagca aatacgacga tcgaaatcgt cacaaaagac gtagatcttt
    21421 tgtctagaca agtagaaggc ttactggtca aaagtaatga actgctcttg gatatcaatg
    21481 gaaaagtcgc aacgatcgat ccattgttca cagcagttgc tgatttaagt gaaagtgtat
    21541 cagaacttaa tcattctagt aaaaacatcg cgacgaaagt cggcggaatc ggtaaaacga
    21601 cagctaaagc gacggttgcc ggaaaagtcg gtgggacagc gatgaaattt tttaaaaaca
    21661 aaaaacatac tgaaacgaca ggaggaaaat aaaaatggct aaaaaaggtg gatttttatt
    21721 aggtgcagta gtcggaggca cagccgcagc aatcgccgcg ttattgttag caccaaaatc
    21781 tggtaaagaa cttcgggatg aattagctgc ccaagcggat gatttgatgg atatggcttc
    21841 agattacaca gatatggcga aagaaaaatc gactgagtta acaggtattg caaaagaaaa
    21901 agcgcaagat ctttctcaac aagcaggtga tctagcaggt agtgtcaaac aaaaagtaag
    21961 tgaatcagca gatgaagcag gaagtgaatc tgacgatatc ttgttaaatc ttaaagaaca
    22021 atcagctgac ttatctaatc gtttcaaaaa aacggcagaa gatatgaaaa aacctgcaga
    22081 tgaattagga agtattgcta aagatacttc agaagatatc atgattgatg taaaagacgc
    22141 tgctggtaaa gcaggagata ctgtcaatga aggaatgtca gaagcaaaac cagttgcaga
    22201 tgactcaaaa gaaatgatga aaatggcaaa agaagatgcg aaagaagcta aaaaagaaat
    22261 aaaagataca atgacagatt catctacaaa tcactaaaat cacaaattta tagaataaaa
    22321 aggtgggagc ggactagctc tcaccttttt tactcttatt tttctaaaac aagtaattct
    22381 tttggtgtag tcgtgaatgg cacacagcca tcgcttgtca catatacaca gtcttcgata
    22441 cgtaccccta ctttttcagg aatatagatt cctggttcaa gagagaagca catcccttct
    22501 tcgattacta agtcatttcc ttctacaagg gaagggaatt cgtggacggt tgttccgatc
    22561 ccgtgaccta aacggtgatt gaaatattcg ccgtagccat agctgttgat cacgtttcgt
    22621 gcgatttgat caagttcacc tgctgtcaca cctggacgga cggcttcttg tgcggcaagc
    22681 tgtgcttcaa gagtgatgtt gtagattttt tcttggaagt cggttggttt ttgataagca
    22741 attgtacgag tagcatcact gcaatagcca ttccaaacaa cgccaagatc gaataacaca
    22801 agttcattcg gtgaaatctt cgtatttcct ggtgtaccat gagggcttgc agcatttgct
    22861 cctgcaagga ctaatgtgtc gaaagacatt tgtgaaacac cttgtttttt caattggtat
    22921 tcaatttctg caatgatcgc ttgttcagtc acgccttctt tcactgcttt aaagccaatc
    22981 tcaaatgcaa catcggccca tttaccagct tcgatcagtt tgtctttttc ctctagagtt
    23041 ttgatcagtt gtagacgttg gatgatggaa gttagatcag tgtaagaatc cgttcccgtc
    23101 aagatatcag agatagcatc gaagcgctcg acagtcaagg cgttcttttc aatccctaca
    23161 ttgccaggct gaccatagcg agcacgaagt tcttttccga tgatctccca agggttttcg
    23221 ctgtctaaat agccacggac atcatacgcc caactactgt tttgagcatc ttcaacttcc
    23281 aaagcaggag tgaaaaggaa aggatcttgt tcaagaggga taaaaagagc tagtacacgc
    23341 tcatgaggat cactttcgta tccggaaaag taagcgatgt ggccagggtc gctgatataa
    23401 gccaattcga cttggtgttg tgccatccat tgacgtagtt cattgatttt tacgttattc
    23461 atttggcaac ctactttcta aattaatttt gtattcaata attgtaccac tgatttatta
    23521 aaagaccaac gttctaaaaa ctgaaaaaag taaaaatttt catgaaaaca gagataaaac
    23581 ggttgcaatt cttgagatta tttgatattc tattttagaa atgtaagacg tattttcaga
    23641 aaacatataa aatatgaata ggagaacaca aatggaaaag caaacaatca ctatctatga
    23701 tgtagcgaga gaggcaaatg tttcgatggc tactgtttca cgtgtcgtaa atggcaatcc
    23761 aaacgttaaa ccagcaacaa gaaaaaaagt cttggaagtt atcgatcgct tggattatcg
    23821 tccaaacgca gttgcgcgtg gcttggcaag taagaaaaca acaacagttg gtgtaatcat
    23881 ccctgatgtc agcaatatgt tctttgcttc tttagcacgt ggtatcgatg atgtggcaac
    23941 aatgtacaaa tacaatatta tcttagcaaa ttcagatggc aacgaccaaa aagaagtcaa
    24001 tgtattgaat aacttactgg ctaagcaagt ggacggcgtg atcttcatgg gtcaccatat
    24061 cacagacgaa atccgtggag aattttcacg ttcaaaaact cctgttgtat tagctggctc
    24121 tattgatcca gatgaacaag taggtagcgt aaacattgat tatgtatccg caacaaaaga
    24181 tgcagtaaac aaattggcaa aaagcggcaa taaaaaaatc gcttttgtca gtggtgcgtt
    24241 gatcgatccc atcaatggac aaaatcgtct gaaaggctat aaagaagcat taagcgaaaa
    24301 taacctacca tatagcgaag gattgatctt tgaagcacaa tacaacttca aagatggtct
    24361 atctctagtc gatcggatcc ataatagcgg tgcaacagct gtctatgttt cagatgatga
    24421 attagccatc ggtattttgg atggtttatt agaccgtggc gtgaaagtac cggaagaatt
    24481 cgaaatcatc acaagtaaca attcactact gacagaagta gctcgtcctc gtttaacaag
    24541 tatcacacaa ccattatacg atttaggggc tgtatctatg cgcctattga cgaaaatgat
    24601 gaataaagaa gaagtcgaag aaaaaacgat tatcttgcct tatggaatcg aagaaaaagg
    24661 atctacaaaa taataaaagg acaaccattc agaggttgtg tgcctgccgt ttaactctgc
    24721 gttacaagga gcaatttcaa aaaacagcat ctcatgtttg atgaatattg tgactcgtga
    24781 ttcggagttg gcaggagaac accgtttatt tgggaaaagg ggttatgaca aaattttgtt
    24841 ataatccttt ttttgtgtct taaaaaagag agaaagtggt tgaaattaca tttatcgtat
    24901 ataaaacaat ccgtgtatag atgctgcatt ttgttcacca ccatcaaaaa aataagggac
    24961 tgtgacaata ctattgtcac aatcccttat tactgtgtat tctattaaat caatcatctt
    25021 tctcatctga ttctttagtt gcttgattcg ctcttgcata agctgaagcg gtgttccagt
    25081 aatctgtata gttttcatcg gtaccgccaa cgccaaattt aaaggcgctt ttcttcggtc
    25141 catttttcgc ccatagcgat tcaactgttt ttcctggtgt ttggatggat tttttattga
    25201 cagtaacttt tccaggtgtt tgaccagtaa atccagatac ttttttcttc atcacatcag
    25261 atgataattc gaatttctca tcagtgccaa aaatgtcagg ttttgcttgg taagcttgat
    25321 tgatcagatt ggcaagataa ttagccgaac gtatccttgc tttgctgtcc attccagtat
    25381 tgtcatcgtg ccctgcccag ctactgatgg taatagacgg agtagagaca attagccatg
    25441 aatctcggta ttcattggta gaaccagttt ttccgaccca atctgctttt cctagatttc
    25501 cgtttaagct tgagatggca tctttgaatg gtgtagtgat ttttgcattg atcacactac
    25561 gcatcatgtc attcatgatc gatgccgttg cttccgaata gatccggaca gagttgcttt
    25621 cgtgtttata aattacattt cctgcgttat ctgtgatgga atcgatgata tagccttctt
    25681 catagacgcc gccgtttgct aaagcctgaa atccgttggt ctgttgaagc gtcgtgacat
    25741 ctactgtacc taaaggagca gattcaacgc cccaattgtc atttgccggg tagttcatct
    25801 ttgccaagta gtgctcgtaa gcatattgtt tcgatccacc ggaattcaac acatcttgat
    25861 ataagtgata agccgggata ttgttggacc attctagtga ctcacggacg gtttggaagg
    25921 tgttcgatcc ttcgttagta gcattgacga ttttctcgcc agcattttct ccttcttgcc
    25981 aagttgttgc ataatctgat acacgggatt ctgatccgat aagccccatg tcgattgcag
    26041 gtccataaac aagaactggt ttgattgatg agccagcttg acgttctgta tcgaaggcat
    26101 ggttattttg attctgactg taattccgtc cgccaacgaa tccgtagatg cggccagttc
    26161 tgttatccat caatacattt ccggtttcta cttgactgcc agcaccatca tctaacatgt
    26221 atccataatt ttcaacgcca ttttgcaatg cagcatagac atctttatcg attgtcgaat
    26281 ggattgtata tcctttattt tggatcgttt gctgtgcctt ttgataataa gcgtcactga
    26341 ccgatgaatc agacatatca gcgttgtctt tttcagccaa ttgttgtgca atgattcttg
    26401 ttgcttcatt catcacagta taatacaaga attctctgtc agtctgttct gcgatttgtt
    26461 gtggtagaaa gtcttttgta aggtcatagg cttttgcttc ttcgtattgt tctttcgtaa
    26521 tttggccttc acgatacata ctgaataaaa caaaatcttt tctggcaagg ccggcagaaa
    26581 gatcttcttt tagcgcgcct gtgttcgtgt atggactata cgtgatcggg ctttggggta
    26641 atccagcgat ataagcagct tgcggcagcg tcacatcttt tgcatctaca ccaaagattc
    26701 cttttgcagc ttcctggact ccagcaatgt tttgtccctt attattacga ccaaatggtg
    26761 agacattcaa atacgtagca atgatctcat cttttgaaaa gtatttttct acttgtgcag
    26821 aaagaatgat ctcgtttgct ttacgtttga atgttgtttc atctgtaagg atctgctgtt
    26881 tgactaattg ctgtgtcaaa gttgatccac cagaagagcc aataccagta gcttcagata
    26941 ctaatgctcg taaaacagct ttaggaacgt aaccttggtg tttatcgaag tattcatcct
    27001 ctgtgctgat aattgcagtt ttcaataaag gtgaaatctt atcagatgaa atagtcgtgc
    27061 gcatcagatc tgtctggatt tttgatatct ctgtattatc tgcataaaca atctttgacg
    27121 tttctgtaat gtttcctagt tctgtctgca gtgctttttt agtcggcaat tgtgtgtctt
    27181 cgacgagata ggcaaaatag ccagcgccta ttccaatacc tagagctgcg aacatcaaaa
    27241 gaagaacagt aataaaaaca gtcaatgatt gaaaaactct aaaaataatc ttaggaacaa
    27301 accatttttc tttttttctt ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg
    27361 tcacctcaac taatggtttt atttcgctaa ttatagcaaa taatagaaca aaaaaacaga
    27421 caaggctgtt tttggcagga atttcttttt taaaagagtt aaaacgtgag aaaatgatct
    27481 ttattataaa agagttctta agcttttctt tagagaaaat ttcagttact atggagaaca
    27541 gggcaaagaa aaatcaatca ttttctttgt atatcatctt accaatatat aggaattgtt
    27601 agtaacaatt gcctttcaag gaggaatcca tccatgttat taggctcaca cgtcagcatg
    27661 agcggaaaga aaatgctgtt aggagcagca gaagaagcag ccagttatgg atcttcgact
    27721 tttatgatct atacaggcgc ccctcaaaac actagaagaa aagccatcga agagatgaat
    27781 attgatgccg gaaaagcgta catggcagaa aaaggtctaa caaatatcgt cgttcatgcc
    27841 ccttatatca ttaatttggg gaatacgatc aaaccagaaa tgtttccttt tgccgtccaa
    27901 tttctgagag aagagattgt tcgtgcggaa gctttaggcg ctactcaaat tacacttcac
    27961 ccaggtgctc atgtaggggc aggaacagaa gccggaattg ctagaattat tgaaggcttg
    28021 aatgaagttt tgacaaaaga acagacggca caaatcgctt tggagacaat ggcaggtaaa
    28081 ggtacggaaa tcggtcgaac atttgaggaa ttagcgcaaa tcatcgatgg agtaaaattg
    28141 aatgaaaaat tatccgttac ttttgacacc tgtcatacga atgatgccgg ttataatatt
    28201 aaagaagact tttcaggtgt aatggaagag tttgataaga tcatcgggtt agatcggctt
    28261 aaagtactcc atatcaatga ttctaaaaat ccgcaaggtt cgcataaaga ccggcacgcc
    28321 aatattggtt ttggaacaat cggattcgac acgctgaata agatcgtcca tgatccaatg
    28381 tttgacaata tttcgaaaat cttggaaact ccctatgtag gcaaagataa aaagaaagct
    28441 tacgcaccat atggaaaaga aatcgccatg ttcaaagcgc aaacattcga tccagaagtg
    28501 tttcctgagt tattagcaga agaagtgata ctctaagatt ttctatctga cagcagcaaa
    28561 aaaggaccag gcacattgcc ttgtcctttt ttgtttaaga aacaaaggag tactttttgc
    28621 agtctttttc atcatgcatc ttctcccccc tctttttaaa tttagacaaa caaggtatac
    28681 tggtagaaaa gtaaaaaaac gaaaagtttc acaagcaagt ggcaaaacgt gattggaagg
    28741 acgaaaagat gctgaacact ttaaaaccct tgatcaacca actaaaaaag aaacatacat
    28801 actttattct tggcatagcc ctattattta caattattcg tttgtattta tatagtaaag
    28861 caatctatgg aacagacatg tcggcaacct atgatgacca gctgctgatt cgctatgctc
    28921 aaaatatttt ggacggtaaa tggctagggg agtatacgac tacaacactt tccaaaggaa
    28981 tctcctacag cttattttta gtacttgcac ataaactgca tctttcttat gcagttttgt
    29041 tcggcatgtt cagtattttt gccagtatat tggttgcact cgcgtttaaa ccgattgtga
    29101 agaacaagta tcttctttta agtatctatt tttatttttt gttttctccg attagtttta
    29161 cacatgaata ttccactcga acatatcgta atgccctcgt gattccggct gttgagatca
    29221 ttatcgcttg tttattagct atttattacc gcaaatacgg ttctagcaga aaattgctgc
    29281 catggttttt agggctttct gtcgcttttc ctttcttttg gttcattcgc gaagattcta
    29341 tctggcttct tccttttact gtagtagctt tgggtatcag tttcgtccag atcttgctag
    29401 ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa acgactcttt cttctctcga
    29461 aacgggaatg gctgaaactt tcattgtttt tacttcctat agttggcttt tccatcgtct
    29521 atcaatggat cgaatataaa aatgagacaa catacggtat tgcaaccatc aatgaccgtt
    29581 ccagtggaga atttggacgt ttgatgaaac aactgatccg aatagatgat gaaacgaact
    29641 tgaataaaga aaacagtgag atatgggtct cacatgctgc actaaataaa gcattagccg
    29701 catctcctac attccatact gtttctaccc gtattgacga attatatacg actcacgcgt
    29761 ggactcaagg tggaaaagta aaagaattac ccggagatat catcttttgg gcattgcgag
    29821 atgtggtaag cgaatctggt tattaccaaa acaatgcaaa agaaaccaat gatttttgga
    29881 aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg aacaatcaaa aagaaaaaag
    29941 agttctatct gatgggatca ggagatggaa agacgatgag tgatctgcca atcgtattcg
    30001 atttccttca atcaggatac gcttatgcat ggcgatataa agatttccat caaggaggaa
    30061 attatagttt cggaacgcaa gaacaagtca aacaggctca agaattatta aaccttccgc
    30121 taatggagaa ttggatcgat agtaatgaac cgagaacacc taatttccaa ttaacaaaat
    30181 cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt tctggctatt cccgtgctga
    30241 ttgcttcaat agcagggata ttacttatga tactaggaac cgtatttgca aaagctgatc
    30301 gtgcctatta tagttcctct ttgattgtgg tcaccggatt aatactgaca caattggttt
    30361 ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga aaaaaaggac tattttttaa
    30421 gtgtctacac tggtgcaggc gtgccattaa cgcaattcat cgtagtaaca agcatgctag
    30481 ttttcagtaa attaaatctg aaaaaagctt tatcttctaa ataaaaagac ccttggtagc
    30541 tagattttta acctaactat caagggtctt tgtgtggaaa aatcagtttt tattcgattt
    30601 cttctaccgt ttcctgcaaa agctgttcat attttttgtt tcctttatat aaatcaccaa
    30661 tcgtccatgc caacaggaga aggacaagaa caatgattac ataagcgatg tgatcagcca
    30721 atgctagttg agcggaagtg gcttgctcgc cgaagaaact ttcgatctgt cctggtaatc
    30781 ctcgtaagtt caagtaagtt agactaatca cagagaacca gccaagaagt tttacgatga
    30841 atgaattttt aaatcggcta cccatctccg cttggctgtt ggtcatcatc agtaaaggaa
    30901 tcatcgaaaa cggcaacgca aaagccaaga acacctggga ttcattcatt aagttattca
    30961 aagcggcatg ttcttcgatc gttccttttg tacttgtaaa caaaacacaa atcaatacag
    31021 gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca aatcgggata cgcatatgga
    31081 tgaatccttc catgatgact tgaccggtca acgtaccagt aatagtagag ttctggccag
    31141 aagcaagaag tgctacagca aataaagtgg acaagatacc agattttgct acagcaatca
    31201 aaataccatt gctcatagct gatggatcag ataaagcttc atacagacca aaaaaggaag
    31261 gatcttggac agaaccggtt ttgaagacag cgacacccat gatcaatagc agtgcattga
    31321 ctagaaaagc cattgacaac tggatattag aatcccaagt agaaaaacgt acagcttgtg
    31381 ctacatcttc ttcgttttgg tgatcgatct tccgagtttg tgaaactgct gaatgaagat
    31441 ataagttatg cggcatgact gttgcaccaa taatccctaa agctccagtc agcggagttt
    31501 cgccgccgat gaccggtgag tctgagaatg tatgactgtt agggataaag ccagcaaata
    31561 aactgtgcca atctgggtta gacaagccaa cttgatagac aaaaacgaat aaaatgacga
    31621 aaattaagca gacaaccagt gcttcgatct tacgaaaccc gacttttgtc agtagtaaga
    31681 gaacgaaaac atctaataca gtaataaaga cggcaatcac caatggaata tcaaaaagta
    31741 agtagagggc gattgctgca ccaatgactt ctgcaatatc tgttgccata atggctaatt
    31801 cagtcaagat ccataaaatg acacctaatg tcttacttgt gcgtgctcgg attgcctgtg
    31861 ccaaatccat ttggctcacg attcctaatt ttgctgccat atactgtaaa agcatagcaa
    31921 tcagactaga catcaaaata actgacatca ataaatattg gaaattttgt cctccggtga
    31981 tcgaagtaga ccagttaccc gggtccatat acccaactgc tactaacgca ccaggtccag
    32041 agtaagcaag aagtgtttta aaaaaacttt tcccctttgg tacatcaact gttccattta
    32101 cttcagctaa agataaacca tttgtatgtt caataagtcg atgcttatga aattgtttat
    32161 tggattgatt attcaagaaa aaaccacctt tcctatttat ctacgattac attctacacc
    32221 tcaaaaagaa aaaattaaag aaaaagattc ggtacgccta attttttttc atggcgcgtc
    32281 ttgctagatg acgaaagaaa ttttgttctc ctctaaaaaa accactattt tttgcatgaa
    32341 actaaagaat acgcctttct tttttcaata aactttagta aaataagtca ttgagagcaa
    32401 ttcggaaata gaaaggagag atttatatgt tatcaacagg tattgtcgta cttattgcta
    32461 ttattgcttt gctaatcggc gcagtaggtg gctttttcct agcgcgtaaa tatatgaaag
    32521 actatttaga aaaaaatcct cccgttaatg aggacatgtt gcgatcaatg atgatgtcaa
    32581 tggggcaaaa accatctgag aagaaaattc gccagatgat gcagcaaatg aagaaccaag
    32641 ggaaaaaata ggttctgacc caacgacttt ttacgggctg attttttcag tccgtttttt
    32701 atttcgtaat ttgttagact attcagatta ttcagcaaaa aagacaagca cagacaatcc
    32761 taggctcttg ataaagacaa aggaggagta aaatgtcgat ttttagaaaa ttaggctggt
    32821 tttttcgtca ggaaaagaaa cattatttaa ttggggtttt ctcgctattc gcagtggcct
    32881 tggtacagtt agttcctcca aaagtgattg ggatcatcat tgatgagatc gccgataaaa
    32941 atttatcaat aaagatcatt ttcgggtgga tcttcctatt gattatcgct gcttttttac
    33001 aatatctttt tcgttatatc tggcggacga atatttgggg aagtgcagct cgattggaga
    33061 aagaacttcg gcagcagtta ttcaaacatt ttacagaaat ggacaatctc ttttaccaaa
    33121 aatatcggac tggggatttg atggctcacg caacgaatga tttgaatgcg atccagaatg
    33181 tagcaggagc tggtatcttg acctttgcag attcagtgat tacgggcgga acaacgatca
    33241 ttgcgatgat tttgtttatt gattggcgct taacactgat tgcgctgatt cctctgcctt
    33301 tgctggccgt cacttcacga gtgctggggt caaaactcca tgatgctttt cgtgattcgc
    33361 aagaagcttt ttctagtatc aatgataaga cacaagaaag tatcactggg atcaaagtca
    33421 ttaaaacatt tggacaagaa aaagaagatc tagcggattt tgaagaaaag attgaagatg
    33481 caattattaa aaacaaacga gtcaattttt tagactcctt atttgatcca ttcattacct
    33541 tgattatcgg tctgtcttat gttgcaacaa ttattttagg tggaaagtat gtcttggaag
    33601 gcacgatctc gatcgggcag atggtctcat ttattagtta tatcggtatg cttgtttggc
    33661 cgatgtttgc gattggtcga ttgttcaacg tattggaaag aggaaatgct tcttatgatc
    33721 gcgtcaatga acttttgcat gaaaagacgc atatcgtgga acaactgggt gcagtgcaga
    33781 ccccggcaaa aggcgagctg aatgtagcta tttcctcttt taattatccg aaagaagaat
    33841 caccagcatt agaacaaatc aagtttgcga tccaagaagg agaaacatta ggtattgttg
    33901 ggaaaactgg tgcgggaaaa acgacgatca tcaaattact gatgcgtgaa tacgatgatt
    33961 atcaagggaa aatccaattt ggcggacgtt cgatcaaaga atattcattg aatgctttat
    34021 taggatcaat cggctatgtg cctcaggatc acttcttgtt ttcgatgacc attcgagata
    34081 atatccgttt ttctaatcca aaactttcag aagaagaagt agagcatgcg gcttctttag
    34141 catttatcaa caatgaaatc aaggaattac caaaaggtta tgatacctta gttggagaac
    34201 gtggagtctc tttatctggc ggacagaaac aaaggatttc gattgctcga gcattgatca
    34261 aaaatcctga attattaata ttagatgatg cactctctgc agtagatgcc aaaacagaag
    34321 aagctatctt gcataattta aaagaaataa gaaaagagaa aacaactatc attgcagctc
    34381 accgtttaag tagcgtgatg catgcaaaag aaatcattgt tgtcgatcaa gggaaaatca
    34441 ttgaacgagg aacccatcat gagctgcttg cactaggcgg ctggtatgaa caaatgtggg
    34501 agaaacagca attagaagcg aagatagaag gaggcgaggc ctgatggatg aaaattatca
    34561 atcggaatgg acaaaatcga ttccacttaa agaacagaaa aagatcatca aacgactgat
    34621 gaagttcgcc aaacctttta gaagaacgtt catcgttgct atcttatttg cgtttgcttt
    34681 gtctgtgatc aatgtcctat tgccaaggat catccaaact tttatggatg atcatctggc
    34741 taaacagtcg gctacgacac aagttatttt gttttttgct ggtatgtact tatttggagt
    34801 catagtaaaa agtatcattt ggtttttcca atggtatttg tattccatgg cctcattgaa
    34861 gacctatcag tatattaggg taaaattatt tgaaaagctg catacactag gtatgcgtta
    34921 ttttgatcag acaccggcag gatcaaccgt ttctcgagtg acgaatgaca cagaaacgct
    34981 atttgaattt tggtacgtct ttttgatggt attgactggg atctttgcag tcgtttcttc
    35041 attccttgcc atgtttcaaa tcaatggaga aattgccttg tggactttgg tgtttttgcc
    35101 gattttagga atcgtcatct ggtattacca gaaattcagt tctcgtattt accgaaatat
    35161 gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa tcgatttccg gcatgcgtat
    35221 tatccagcag tttcgccaag aagctcgttt agctaaagaa tttgaacaag tgaacaatga
    35281 gtatttagac acgcgttatg cgatgatcaa aacgaattcg attctgttaa gtccgatcat
    35341 caattttcta tatgcattgg cgattgcgtt ggtgttgact ttgtttggga tcgatgcgtt
    35401 gcattctccc gtagaagttg gactgatcta tgcatttacg acctatgtcc agggattttt
    35461 taatccaatg tctcaaatga tggacttttt gagcatcttc acagatggga tggttgctgg
    35521 tagtcgtatc ttgaagatta tggatacgga agaatatgca ccacaacaaa accctggagc
    35581 agatcaaatg attacttcag gtaagattga gtttcgcaat gtaagctttt cttatgatgg
    35641 agagcacaat gtattaaaca atatctcttt tgttgctaat ccgggagaga ccgttgcatt
    35701 agtgggacac accggaagcg gaaaaagctc gatcatcaac gtattgatgc ggttttacga
    35761 attttatgaa ggtcagatat tgattgatga tcatgacatt cgagaatatc caatggaaga
    35821 gttacgtaag aaaatggggc tggtcttgca agatgctttc ttgttttatg gggatatcgc
    35881 aggaaatatt cggatgatga atccagctat cacagacgaa cagatacgcc atgcggctga
    35941 gtttgttcaa gcaaacaaat tcatcgatga attaccaaaa ggatatcact caaaagtgat
    36001 cgaacatggt gccagctatt ctagtggaca acgacagctg atcacttttg cgcgaacaat
    36061 cgtgactgat cctaaaattc tagtattgga tgaagcaaca gcaaatatcg atactgaaac
    36121 agaaggattg atccaagaag gattggcaaa aatgcgtcaa ggacggacaa cgattgcgat
    36181 tgcccatcgg ctgtcaacga ttcgagatgc agaattgatt ttagttttag acaaaggtca
    36241 tatagtagaa cgcgggaatc atgaaacact tctagcgcaa aatggtttgt atgcagatat
    36301 gtacaaactg caaagcagtg gtatataaaa gatgttgtga cagtagaatt ttgacctaag
    36361 cactagagtg aaaataaaac atagcttctc atatttttca tctgtgagag ttagtgtcgt
    36421 agtcaccaga tttaaaaaaa gattcgacag ctagtcagaa gtgcctaata gccactaaga
    36481 aagcgttgac aaaagcattt gctttcgata atataaagaa aacgatacaa cttggatagt
    36541 gattgcctga tgcaagctaa acctgatgag tctcccatgg tttcatggac ggctttttgg
    36601 gtttttcttt ttgttttgga tacagcccta tacatcatgg aaaggagcaa aacatgctga
    36661 ttgagaaagt actgaataac aacgtcgtaa taaccaggga tgaaagaaat caggaaatga
    36721 tcgtgatggg aaaaggtttg gcattcaaaa agaaagtggg tgacccgatt tttgactcat
    36781 gcatcgaaaa aaaatttcag ttgacaaatg acgggctttc acaacagttc caagaattgg
    36841 cacaagcgat tcctcttgag tacttagaaa tttcttgtga aatcattgaa tatacaaaag
    36901 aggctctaca agtccagttg aatgattctg tgtatattgc attgacggat catttgtata
    36961 cggctatcga acgtcacaaa atgggaatat ttgtaccaaa tgtattgctg tatgatatca
    37021 aacaactatt tcctaaagag tatgccatcg ggaaaaaaac agtgaagaaa atctcggata
    37081 aataccacat caagttatca gaagatgaag ccggatttat cgcattgcat ttagtcaatg
    37141 cacaaggcat tcaaagccag caagacaata cctacctgtt tactataaca atacaggata
    37201 ttctaaatat cgtccgctat tactataaga ttacatttga tgaagattca gtccattttt
    37261 ttcgattcgt cacacactta cgtttttttc tgacacgcgt cctagatgga gaaaaatcag
    37321 aagtgggaga agtttctgag gaattgtttc aggttattca aaaaaaatat aaggatacgt
    37381 ttggttgcgt tgagaaaata gcagactttt tagccgagac gttcaactac cagatgtcca
    37441 cggacgaaaa aatgtattta acgatccata ctgcgcgttt agtgcatgag aatcgttgta
    37501 aataaaaaaa ggaggataaa ttaacaagaa cgtatgaaat aagggtagtg acattcagtt
    37561 ggctaaacct tccactaata aattggaggg aatagaaatg ggaaaatacc aagaattagc
    37621 gagaaaaatc gtagaaaatg tcggcgggaa ggaaaatatc aatggtttga cacattgtat
    37681 cacaaggctt cgtttcaaat taaaaaacga agaaaaagca aatgatgaga tcttgaaaaa
    37741 tatggacggt attgtaacgg taatgcgagc gggagggcag tatcaagttg tcattgggaa
    37801 tcatgtgcca gtcgtgtttg aagaggttat aaaagcagga aaccttactt ttgatgaggc
    37861 tgtttcaaca gaaaaaatga gaccgtttga tatgcttatt gatggaatat cgggctgttt
    37921 ccagccattc cttgctatcc tagctgctgg cgggatgatt cgtggactga cagcattcct
    37981 tgtcttctta ggcgcgtttg accgcggttc tggcacattt gttatgtttg ataatatcgg
    38041 tgattctgta ttccaattta tgcctgttat tatcggttta acagctgccc gtaaatttaa
    38101 agtaaatgaa ttcgtaggga tgttgcttgg cgcagcatta atgaatccta gcttgtcgct
    38161 ggaagctttg agtggtgcag cagaagcacc actaacgacc atattttctg gcactatttt
    38221 tgaagcacct atttatcaaa cagtattcgg cattccctgg attgcgcgta actacgcttc
    38281 ttctgtcatt cccattatct ttattgtgtt gttagctagc caaattcaga aaccaattaa
    38341 aaagttagta cctgagatga tcgcgaattt cttcgttcca ttcttcaccg ttcttattac
    38401 aatgccttta ggttttttac tcgttggacc agtattcaca ttcgcaactg atattttaat
    38461 ggctggattt gaaacacttt tagcactttc gccagttatt tatggagcaa tcgtaggctt
    38521 cttctggcaa attctggtga tgtttggtct ccattgggca atcgtaccaa tgggattgat
    38581 gcaattttct gtgaatggtt ggcaaaatat tatgacacct gttgcagtag tgtcttttgg
    38641 acaaactgca gcacttaccg cactctactt taagcttaga aatccaaaag acaaagcaat
    38701 tgcaatacca gccattgttt ctggtattgt ggggattaca gagccagcta tttatgggtt
    38761 tacattacca cgtaaaaaaa tatttatttt tacctgtgtc ggtggtgcgt tagggggcgc
    38821 gtatagtggg ttaatgaatt taacttcttg gaatcaaggt ggactaggca tctttacaat
    38881 tcccaactat attagaccag atggtgattt aacagatgtg attaatgtat tgattgggat
    38941 cgccattgcg atggtggtat cctttacgct gacttttttc ttttggaagg atgaagcagg
    39001 tgaaacagat gatatacaga aaaaatcagg taaagaaatt gtaaaaacac cgatacaagg
    39061 acaaatcgct ccactaaatg cagcaaagga tgcagctttt gcacaaggaa cactaggtcg
    39121 aggaatcttg atctaccctg aaaaaggaga agtacgtgcc ccttttgacg gaacagtcat
    39181 gaccctgttc ccgacgaaac aggcaattgg aatggtttct gaaacagggc tggaattgct
    39241 gattcatgtc ggattggata ctgtgcagtt ggaagggaaa tattttgaaa gcttagtgca
    39301 gcaaggataa agagtgaaac aaggcgatct gttgctacgc ttcgacaaaa agaaaatcga
    39361 aaaggaagga tacagtttag aaacacctgt gatcgtcacc aattacacgg attatttgga
    39421 tattttggaa aaccaagggg agacggtcgg gccagacgat gcacttatca ccgcactgac
    39481 ctgaacggtt aggtgaagaa tggactaggc ttaactattc tttttattcc acttctttat
    39541 tttcaaaatt gaaaggctct ttcatgatgg ctgagctgat agggcgttta tcgaattttt
    39601 gaccgacaga aattagatgg actaaattct gttgataatt ttctgcaaaa acacaactat
    39661 ataacacgtc tagaagataa caaattgatg tattgatcgt aaaattacca atttttgaat
    39721 acagtcgctc gcgtgtcgta agtcgcaaag cggctgtact cattgcagat agcgttgaat
    39781 caccgatact ggtcaaagct aaaattggaa catgattttg tttcaatatt tctgcggtac
    39841 gaaggatcac attgttttca cccgtataag aaatcagaat cccgcacata tcactcgtgc
    39901 aattgtacgc ttcatagaat gattcaccaa aagtggtgac aatcgtgaca tccattttga
    39961 tgcgcttcat tttcagcatg aaatcttgag aaatcaaagt attcgagtta ctggcgaaaa
    40021 tcacgatttt ttttgcttga agcattagtt gtttggcttt ttgcagttgg tcatgatgaa
    40081 tgagagaaag tgtatcatga atcgtcatct gttctaaaga agccatttta tttgcgatcg
    40141 tcataatacc gtctgtggtg gaaaaaggaa gattcgcatc tacgtcttga aaattccctt
    40201 caagataggc tgtttcagct aaaaaagcat tacgaaaact gacccagcca tcaaaaccca
    40261 ttttttttgc tacacggatg agtgtagagg ggtggacata acatcgctca gcaatcgctt
    40321 ggatggtcat ttcgccaagt gctggagaat cgcttaagag aaactggatc acttcatttt
    40381 ctgaaggaga aaaggagatt tctttgattt tttcacgtaa taacatgtag ataccttctt
    40441 cttatttttt tacgtaaatt ataacttttt tgaaaat
//
LOCUS       DAZKCY010000014        38908 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000014 DAZKCY010000000
VERSION     DAZKCY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.014"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..135
                     /locus_tag="ACG8XC_000649"
     CDS             <1..135
                     /locus_tag="ACG8XC_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9190370.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(152..691)
                     /locus_tag="ACG8XC_000650"
                     /pseudo
     CDS             complement(152..691)
                     /locus_tag="ACG8XC_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(688..2583)
                     /locus_tag="ACG8XC_000651"
     CDS             complement(688..2583)
                     /locus_tag="ACG8XC_000651"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9190371.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(2641..4416)
                     /locus_tag="ACG8XC_000652"
     CDS             complement(2641..4416)
                     /locus_tag="ACG8XC_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP9190372.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(4466..5962)
                     /locus_tag="ACG8XC_000653"
     CDS             complement(4466..5962)
                     /locus_tag="ACG8XC_000653"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9190373.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(6156..7778)
                     /locus_tag="ACG8XC_000654"
     CDS             complement(6156..7778)
                     /locus_tag="ACG8XC_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9190374.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(8227..9540)
                     /locus_tag="ACG8XC_000655"
     CDS             complement(8227..9540)
                     /locus_tag="ACG8XC_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP9190375.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(9785..10009)
                     /locus_tag="ACG8XC_000656"
     CDS             complement(9785..10009)
                     /locus_tag="ACG8XC_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190376.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(10029..10451)
                     /locus_tag="ACG8XC_000657"
     CDS             complement(10029..10451)
                     /locus_tag="ACG8XC_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9190377.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(10594..11304)
                     /locus_tag="ACG8XC_000658"
     CDS             complement(10594..11304)
                     /locus_tag="ACG8XC_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFP9190378.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(11322..11819)
                     /locus_tag="ACG8XC_000659"
     CDS             complement(11322..11819)
                     /locus_tag="ACG8XC_000659"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9190379.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(11856..12308)
                     /locus_tag="ACG8XC_000660"
     CDS             complement(11856..12308)
                     /locus_tag="ACG8XC_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9190380.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(12443..13048)
                     /locus_tag="ACG8XC_000661"
     CDS             complement(12443..13048)
                     /locus_tag="ACG8XC_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP9190381.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(13498..14118)
                     /locus_tag="ACG8XC_000662"
     CDS             complement(13498..14118)
                     /locus_tag="ACG8XC_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9190382.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(14120..14989)
                     /locus_tag="ACG8XC_000663"
     CDS             complement(14120..14989)
                     /locus_tag="ACG8XC_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9190383.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(15012..15419)
                     /locus_tag="ACG8XC_000664"
     CDS             complement(15012..15419)
                     /locus_tag="ACG8XC_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9190384.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(15438..16727)
                     /locus_tag="ACG8XC_000665"
     CDS             complement(15438..16727)
                     /locus_tag="ACG8XC_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9190385.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(16754..17056)
                     /locus_tag="ACG8XC_000666"
     CDS             complement(16754..17056)
                     /locus_tag="ACG8XC_000666"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9190386.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(17081..19174)
                     /locus_tag="ACG8XC_000667"
     CDS             complement(17081..19174)
                     /locus_tag="ACG8XC_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9190387.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(19663..20427)
                     /locus_tag="ACG8XC_000668"
     CDS             complement(19663..20427)
                     /locus_tag="ACG8XC_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFP9190388.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(20538..21407)
                     /gene="mdcB"
                     /locus_tag="ACG8XC_000669"
     CDS             complement(20538..21407)
                     /gene="mdcB"
                     /locus_tag="ACG8XC_000669"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFP9190389.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(21432..22508)
                     /locus_tag="ACG8XC_000670"
     CDS             complement(21432..22508)
                     /locus_tag="ACG8XC_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9190390.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(22532..23125)
                     /locus_tag="ACG8XC_000671"
     CDS             complement(22532..23125)
                     /locus_tag="ACG8XC_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFP9190391.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(23129..24805)
                     /gene="mdcD"
                     /locus_tag="ACG8XC_000672"
     CDS             complement(23129..24805)
                     /gene="mdcD"
                     /locus_tag="ACG8XC_000672"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFP9190392.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(24795..25094)
                     /locus_tag="ACG8XC_000673"
     CDS             complement(24795..25094)
                     /locus_tag="ACG8XC_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFP9190393.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(25196..26842)
                     /gene="mdcA"
                     /locus_tag="ACG8XC_000674"
     CDS             complement(25196..26842)
                     /gene="mdcA"
                     /locus_tag="ACG8XC_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFP9190394.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(26849..27760)
                     /locus_tag="ACG8XC_000675"
     CDS             complement(26849..27760)
                     /locus_tag="ACG8XC_000675"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9190395.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            27880..28737
                     /locus_tag="ACG8XC_000676"
     CDS             27880..28737
                     /locus_tag="ACG8XC_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9190396.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(28924..29286)
                     /locus_tag="ACG8XC_000677"
     CDS             complement(28924..29286)
                     /locus_tag="ACG8XC_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9190397.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(29475..29657)
                     /locus_tag="ACG8XC_000678"
     CDS             complement(29475..29657)
                     /locus_tag="ACG8XC_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190398.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            29632..31071
                     /locus_tag="ACG8XC_000679"
     CDS             29632..31071
                     /locus_tag="ACG8XC_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9190399.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(31078..33630)
                     /locus_tag="ACG8XC_000680"
     CDS             complement(31078..33630)
                     /locus_tag="ACG8XC_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFP9190400.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(33744..35075)
                     /locus_tag="ACG8XC_000681"
     CDS             complement(33744..35075)
                     /locus_tag="ACG8XC_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9190401.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(35041..36519)
                     /locus_tag="ACG8XC_000682"
     CDS             complement(35041..36519)
                     /locus_tag="ACG8XC_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9190402.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(36824..38659)
                     /gene="tdc"
                     /locus_tag="ACG8XC_000683"
     CDS             complement(36824..38659)
                     /gene="tdc"
                     /locus_tag="ACG8XC_000683"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP9190403.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        11388 a         8256 c         6512 g        12752 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tatttttata gcgtttgaat actgtttcag
      181 cgaaacgaac gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca
      241 aatataacgg catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac
      301 attcgtcaaa cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac
      361 catgaggaaa aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag
      421 caaataaacg gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat
      481 aataatactt gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc
      541 attgatctgt ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag
      601 ataatccttt ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc
      661 cccataaaaa tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct
      721 tgtttgatta tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac
      781 aataaccatt gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat
      841 caataaatcg cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc
      901 atttaaattc actgtatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac
      961 taccccaatt gcatgtttcg ttggaaaaac tgttgaaact tccccgttta caggactgta
     1021 gatttttccg tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt
     1081 tggatcgttt acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat
     1141 atcctttgag gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca
     1201 ggaactgatc atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata
     1261 aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc
     1321 agctactcca ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt
     1381 aagagtcaca ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga
     1441 taaagataaa ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc
     1501 tgcttgcgca gctacggcag tcatacatgc aggacctagc gtatcacgac cgtagaaaga
     1561 aagattgttg gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg
     1621 ccaaaatccg ccgataacag caccagataa aacagggcta aagttataaa cagctaaata
     1681 tccagctgca atcagctgac ttgcataatc tgcaattgga cctacaacta ataaagtaat
     1741 tgggaccatc acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa
     1801 gcttttgcga atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat
     1861 aaccgtactt gtataccttg ctagaatcac gggcatccct agaaatcgca aggttatgct
     1921 atcactatat gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat
     1981 gaaacgatct gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata
     2041 gaaaaatgca tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa
     2101 attcaaactc gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc
     2161 taaagtcggg gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt
     2221 ctcagttcct ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac
     2281 gtcatacact tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac
     2341 tacagtgatc acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt
     2401 tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc
     2461 gacaaatcgg acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt
     2521 aattaatgcc gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc
     2581 cattcaatta gtacccctta taatagcgaa cgaaactgta caatagtgtg tatggtttac
     2641 ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc
     2701 aaaggagaca aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag
     2761 ctgatactct gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc
     2821 ggcatttaac ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc
     2881 tatcttttta tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt
     2941 tttctttttt tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctcccaaga
     3001 ttcaaactca tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc
     3061 ttgctcagta gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag
     3121 atacactggt tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt
     3181 ttttagtgta ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt
     3241 tcgaaaagga ttcaacgctt cggcaagata caaaacaaaa taagcgcttt cagctaaatt
     3301 gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata
     3361 acgggagatg atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt
     3421 tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg
     3481 aagatctaaa aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt
     3541 gtttttttca tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg
     3601 tttttcttga ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc
     3661 taccatcgga ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag
     3721 aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt
     3781 atatgatata agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat
     3841 tgtactgatt tgttgaggca accttgcgtg ttggatcacc gtttctgttc caacaagaga
     3901 taagaaaatt ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac
     3961 ttccagttct aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga
     4021 tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt
     4081 ttgaaacaat aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt
     4141 atgtttcgtc acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa
     4201 ataacctgaa cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt
     4261 tttgatttca gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct
     4321 tgttatccaa tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa
     4381 gtccatttgc ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca
     4441 atagtcactt gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac
     4501 cattagactg gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc
     4561 ttccgctccc atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc
     4621 ctgttcctgc cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac
     4681 catcttttat tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg
     4741 gatcgtggat tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta
     4801 tgaataatgg taattcataa cggtctgtca attcattcat gacataacga aaccctagag
     4861 gatcaattgg ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac
     4921 catttgctcc gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat
     4981 aataactaaa tgcaatataa tctgccgtgt tagcttgcaa gcactgttcg tctccatcta
     5041 acattactgg tgcacagtct tcttccttcc atgtcctgta aatataagct ggataatgcc
     5101 ctcggcacat gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata
     5161 cattctctgg atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac
     5221 ccatcttagc atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa
     5281 acatatgatg acaggcttga taaatcactt tttttgttag tgtagcaatg ggttcaagtt
     5341 tatctctagc agggaatgta accaatccag cagctgcata aggcatacga aaagcattat
     5401 tgatctcatt aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct
     5461 gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta
     5521 ccaatgctaa gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat
     5581 ttaatgtatt gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt
     5641 ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact
     5701 ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa
     5761 gataatggag tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa
     5821 tactaggatg ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt
     5881 cttccaacca tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt
     5941 ctggaaatat ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa
     6001 gtatagctcg attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa
     6061 attgaatgct actcttcatt tttaatattt atatataaca aatacataag acgatccccg
     6121 aaagaaaaca ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct
     6181 tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca
     6241 ataaccctta aagcttcacg tggaacagtg aacgtctgct tttcactgga catattacaa
     6301 ataacgagcc attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc
     6361 ttttccacta attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc
     6421 aatttcttgt acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt
     6481 tctttataat ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca
     6541 cgggcgttcc attgcatagg cgtcctagca ttgtctcttc ctttcgcatt gattgattcc
     6601 aagatttcct cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca
     6661 atatcttttg cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct
     6721 tgatagatat aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt
     6781 tcttttcgat acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta
     6841 ttccaaaata aactattcca tccttcatca cctaaagatg tttgccattt ggataatact
     6901 tttttcagtt cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga
     6961 tctaatccta tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa
     7021 taaagtttag cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg
     7081 tcaccaaaag ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt
     7141 gtgatttctt tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccccca
     7201 atacctttat cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt
     7261 ttccaattca aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt
     7321 tcatccaact gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca
     7381 ttgaccggat cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc
     7441 tcaatgaacc acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg
     7501 atatttcttt tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct
     7561 gccatgattg cttgataatt actgatatca tacccattgt catcattcgg agactcgtat
     7621 actggagaaa gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata
     7681 atcccccgca aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt
     7741 tgataaacaa ctgaatggtg ccaccatttt tgttccatga ttttcctcct ataaatggat
     7801 gtttctattt tttgcttaat catttatttt agtctctctc actaaaaatt gtaacgcttt
     7861 caaatagcta ttgcaccttg ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa
     7921 ttccctcaac tgttttttga aaggattgat ttatgcaggg aagaatgctg atttcacaac
     7981 ttctttataa acggtgttca ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat
     8041 atgaggaact attttttcgt ttttttctct aatactcagg ccagaacact gatttcacaa
     8101 cctctttata aacggtgttc actcagctgt ccctcggtat tagctcaaaa aacggcaaaa
     8161 tataagaaac tattttttcg tttttttctc taatactcag gccagaacgc tgatttcaca
     8221 acctctttat ttcacattca atatccacat gaatcccaca aatacaaaga acagaccata
     8281 catgattgga gatacttctt ttcctctttt agctgctaac atggtaattg gatagaagat
     8341 aaaacctaat gcaatcccat ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa
     8401 gaatgaaggg atcgcaatct ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat
     8461 caatactcct accataatca atgctggtgc agttacttgt gaagtaacaa ctgatagtaa
     8521 tggagaaaag aacaagccta acaagaagaa aatccctgta gtaatagctg tgatacctga
     8581 acgtcctcca acggcgatac cagctgatga ttctacaaat gcaccaacag gagaagtacc
     8641 aaataaggaa cccgcaagca tagctgttga atcagaagct aaagctttcc ctacacgagg
     8701 catcttgttg tctttcatga aacctgcttg gtttgctaat ccaactaaag ttcctgctgt
     8761 atcaaagaaa gtcacaagca agaatgtcag cactacgacc cacatttgaa gagaattgat
     8821 gtcccctaca tgttttaatg caactaaaaa tgttggtttc aaacttggtg ctgcggcaac
     8881 aatttcagaa ggcattggaa tcaaacctgt gaccagacct aaaatcgttg tagctgccat
     8941 cccgatgaag atgccacctg gtacacgccg aacaagcaag atagcagtta cgactaatcc
     9001 aaaaatcgtc aaccaagtag aaccaacgtt gagtgatcct aatcctacta atgtagattc
     9061 attcgaaacg atgatcccac cttcgctcag accaaggaaa gcgataaata gaccgatacc
     9121 gccagaaatc gcatatttca aatctgctgg gatcgcatca atgatcattt cccgcagctt
     9181 gaagattgtg atcaaaataa aaatcaaaga agcaacgaat acgccggcta atgctgtttc
     9241 ccatggaatc cccattccta aacaaacaga ataagcaaaa aaagcattga tcccaagagc
     9301 aggtgccgtt ccaattggat aacgggctaa tacccccatt aaaacacagc cgatcgcact
     9361 ggctaatgca gtcgcagtaa acactgctcc ttcatccatt ccagaagcac ctaatacagt
     9421 tggatttaca aataagatat aagccattga gataaatgtt gtaaatcctg ccagtacctc
     9481 acgttttacc gtggtgttca atttttctat ttcgaagtag gaaagtattt tttccttcat
     9541 cttccatccc cctaaatccg aacattataa ataaagtccg ttaaaatttc gatttattaa
     9601 taaaacaacg tgttttatca tatactaaaa caaaacataa gtaaacaaaa aattcggatt
     9661 ttaagcaaaa tccgaacgtt ccatctgatt aaatcccttt atctcaaagt tcatgaacat
     9721 tcactttgga ttacaagaat ttctttattg tgttaataaa gtttcttcat tctagtctca
     9781 aaaatcaata gtgtgatttg attttaccat ttcgtttctg ttcttctttg atgaattctt
     9841 ttgtatcgac aggattcaga ttatttagtc cgccaaaacc tgtatcgacc atttttcccc
     9901 agccttttgt cttctttttt cctgatttga gattctttgc tcgaagcttc tcactatgtt
     9961 ttgctcgccg atacttcaaa taaccttcta atcttcccat tagatccact gtttttcctc
    10021 ctttttattc aatcagacga ctcagctttt cctataacaa aataattgcc ttcagggtca
    10081 gaaaaattga aagtcaagat gccattgtta tctgtgattt ctcctggttc ttccatgttc
    10141 tcgtagatag tttcaaattc atccgtaaaa aatactaaag aaggcggtgg tcctaatacc
    10201 tctggtgaaa acttctgcac aaatactttt gggaatacgc ctaattctaa ttcttttgag
    10261 atgctgagta caatactctt aaattcttct ggcaattcga ttgtttcaga aatttctgca
    10321 ccaatttttt ctacaaagaa tcggcaaatc atctctacgt cattgacata aagcatcaca
    10381 cgtgtttttt gtatcatcat ttgacccttc tttctatttg attttctttc ctgtctataa
    10441 actgattcca ttatatcacg aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa
    10501 taaagcaata gtttctagga ttgctttcct ccacaaactt tttttacaaa aaaaaaaacg
    10561 gcaatgaatg ccgctcttct tatttcgatt cttttataat tctgcttctg attcatcttt
    10621 ggttagtgtg tgtctaacac tagttacgcc aaatgtttga tcgatttttt ccttcaacgc
    10681 ttcgattgcc tgtgcgccac gtgccgcttg ttcagcattg atggattcaa tgaccagcac
    10741 cgctgcttgt gtttcttccg tcagcattgt tctttgggct tcacgccagt tgagacagcg
    10801 acagatcgct ccttgttcat catagtagat gatttcttcc ggaagtgccg gagcatcgga
    10861 atctgcacct aaaggcagga atgattcgcc accttgggct tttcctaaat gcagatttcc
    10921 tgcgatcttt tcgatatctt ctccgccgca aggtacgcca aaagacaagg aaatactatt
    10981 atagatatct acaagcggat tgattgggaa aaattcccgt tcttgatttg cacgtttcag
    11041 taaagcttca atagaagaac gtgcaccttt tttagtcttg aatgtggtaa atgcctgacg
    11101 ccactgagca atgacttcgt tttggctgaa tgtttcttct gtaaggtaat ttcttgcttc
    11161 ttttgctgcc tcattcaata attgatgata cgtttcttca ctttcttttg ttacgtggtt
    11221 atcgatacca tttacggata agatttcgat ggttgcttca ggaaatactt cccagaaatt
    11281 ggtatccact attacttttt tcatctgttc acacctcttg attagaataa ttgcttgatg
    11341 aatagatagt ctgtttgggg atcatctccc atataaaaaa catgttcgct gtatttgata
    11401 aatccttttt tacgataaaa agcgagcgca acatcgttgt gttcccaaac accgagccaa
    11461 atggctgttt tttgcttttc ttttgctttt tcaatagctg tatctagtaa aaatgtaccg
    11521 ataccttgat gtttaaattt ttttcgtaca tagattcgct cgatttctaa aaaatgatca
    11581 gccatttcct cagattgact cgttcctgta tttagtttca ggtaacccgc aggctcttga
    11641 tcgatactca ctaggaaaaa atcggtttct ttatctttta gctcttctgt aagtttttct
    11701 agtgcataag cttgtgtcaa gtaatcagct aaatgctccg gtgaattatc ttttccaaat
    11761 gtatctgtaa atgtttcgat actaatttgt tgtagtaagt ctacatctga aggcgtcatt
    11821 tttcgaattt cgtatttatt cacggcttct tcctttcaat agatcctttt attgcctttt
    11881 ttgacaaaat tccaatcttc gctaacattc ttgcatactt tatttaaaag atattccaat
    11941 tgttttgctt cttcgtcaga aagaccattt aatgcgactg cattggagta gttgttttct
    12001 cgaatgatga aaggatagat ttctgctcct tttttagtcg gatagagttt cttgattttt
    12061 ttattttctt tatcttccaa acgttcgatc atcccattgg attctaattt cttgattgct
    12121 cgggcagtcg ttgtacgatc tactttgatc atttctgcta gtttttcttg gatgattcca
    12181 ggattttcgc aaatacgtac taaataaaga tactgtcctc ttgttaaatc gacttctttg
    12241 aattcgatat tgctaataga atctagcgct cgggcaatca ttccgatgtc tcgaaggatt
    12301 tcagccattt catcccacct ttattttgtt tgtaagacta gtttacctct ttttgttgca
    12361 aatgcaacta ttttcaataa aaaaatcaag ttctttttta ataactagta aagtatattc
    12421 ttttcttctt ttacgaaaaa gctcaatcac aatagatctg gtttttgttt tctaaaataa
    12481 gtaactgttt cttcgttgtt cgcttttctg atagctttgg caactcttct atactaaaaa
    12541 atcccatgtt ggaagtttca ttattctcaa taaacttggc ctcttcaccg tggatcgcac
    12601 aagcaaaaac aagtttataa tactgaaaaa gctgtggaat atctttttgt ttattcgtat
    12661 caaaaactgc tcgcaactga gtagtttcta ctgttaatcc tgtttcttca tagacttctt
    12721 tacgcacatt ttcttcagga gacaatccta tttcagcaaa tccaccagga agtgaccatt
    12781 ctttcgtccg tttatcttcg attagtagga ccttgccatc ttttttgata taggcacgaa
    12841 catctacttt tggtgttgga tgtccttctc caatcgttgt gagcgctttt atttcttcga
    12901 aagtttcttt tcctatttca ctgatcagtt ccaatgaaat cgtccggatc tcctcaaaac
    12961 gctctttatc aaatacatct tttccataat atagaccagc ttgagaaagt gcgagtagtc
    13021 ttttgtattg agccaacttc ttgttcattc tcttcccttc ttttttagca aatgtatgac
    13081 tatgtaatca agtatagaag actggtttaa ttacggcaaa taaatataat gaaatctgca
    13141 ataaataaaa gaatgttaaa aaactcgaga acaaaagaca ttcgacttct ctggggctgt
    13201 gaataaatgg cgttcaaaag cttgcatctg cggtaataag ccccaccaaa cgaaaaatat
    13261 gacgagctat tcttgtctgg ctgttcttat taattgaggc aggacagctt tttcacaacc
    13321 tccccactaa acggtgttct cctaccaact cctcggtcac aagtcacaat attcatcaaa
    13381 catgagaagc tgtttttttg aaattgctcc ttgtgactcg gagctgaacg acaggctcac
    13441 gacctctcgg taaacagcgt tcaaaagcca atttttcgat attaagccat ttctttgtta
    13501 cttattgtca ttgatttctt tttctaattt atccaatagt ggagtgtaat ccacttgagt
    13561 atagtctgtt acatcaaatt catatagtgt tcctaactgg aatttgtcat attttcggtc
    13621 ggcgatgatc tttttgaatg cttctttcgt aatatgattg gtccctttcg ttcgatcttc
    13681 caatacgtca ccatagtgat aatgatccat gatatagctc aaatgacgat ctgattcacg
    13741 atcacgtttg taccgacgtt gacataacac ttcaaagtct gctgttaggc gaatcgtgat
    13801 cacctgatat ccgtatctat ctgctaattc ttggagtcgt gctttttgct tatcactaaa
    13861 aggatactcg gacacaatca ctttctttcc tgcttccata tacaaatcaa gtgctttgta
    13921 atagaaagga taaactttcg tcctttcttg ttccgcccgt tcttccaaat tgttaaaccc
    13981 gatggattct gcatagtatt ccttgatttc atcgagcgtg atggcataca tatctggaaa
    14041 tcgttcccgt agtttattca taagatatgt ttttcctgta ccaggactac cagccagaag
    14101 aatgaaatac tttttcatct catttatttc ctaagttaat tggactttcc aagttcataa
    14161 acagtccaat cttatgcttg acaatttctt ttactgcttc atttgctgga atcacattat
    14221 ggcgaagaga cttgttgatt tctgtttcac taaaggtttc tttagctact ttcgtccagt
    14281 tgactaaaag ctccgttcca acattgaatt tgcggatccc gttattgatc aattcaggat
    14341 aatcttcttc tttcacacct gttcctccat ggataactag ggggacatca actactgcat
    14401 cgatttcttt cagcaaggga atgtttactt ctgtttttga tttgaattgt ccatgattcg
    14461 taccgatagc aatagctaat gcatccacgc ctgtagcttc tacaaatttc actgcgtctt
    14521 ccggtcttgt atataccttg tcattttcag cgacatggat tccttcttct gtaccgccga
    14581 ttgttccaag ttctccttct acggaaacac cgtgcgcatg ggcatattct actactgctt
    14641 ttgtcttcag gatattttct ttgaaaggca gatgggaccc atcatacatg acagaagtat
    14701 atccgctgtc gattgcttct ttgatatcat caaagtctcg ggcgtggtct aagtgcaaga
    14761 ctgcatcaac catttcttct tctgcaatag ctgtcattac attgactaac acatgcatgc
    14821 cgatatattt agcggtatcg acacttgttt gaataatgat cggcgaaccc atttcttttg
    14881 ctgcgcctag catagcagga agcatttcta ggttatgcgt attaaaagct ccaactgtaa
    14941 aattcaactc ttctgctgtt tttgttactt ctttcaatgt tgtatacatc ttttattgcc
    15001 accttatctt tttatttgtt tcctttgatc gttacttgtt ttgcaatttg tcgcatctca
    15061 tccattttgc ccatgtagta atcgatccct tgatcatcac cagcacgagc agcatctgca
    15121 cgcttctggt tatagaggct catcagtttt ttatacccat ctccgatcgt ctgtttattt
    15181 tccaaactct tttctaaata ttggatggct tgatcgttca catacaattc agaaaattta
    15241 agtccaactt gttcataaag tttcgctaat ttatgcttat cttgggtagt ggaaatctct
    15301 tgtttcaatt gtgtaatctc tgattgaatc ttttcggtcg cttctggcga tagggctttt
    15361 tcttcttttg ccagaacttt tttcggttct ttttcttttt tcttaaaaaa tccaaacatc
    15421 attttcccta ctttcaatta aaatgctttc aaaactgggc ttaagatcca ggcatataag
    15481 agtgctgcgg ctactgtatc tacatcggtt gctgtagcat tgatgaatcc catcgaatta
    15541 aagatcgtta ctagcaaagc gggcaataat gtaataaaga aaccatgagc aataccgcca
    15601 atgatcgctc ctcggcgtcc gccaactgcg ttgccaaata tccctgctgt ccctccggca
    15661 aagaaattgg ttagcatacc aggtaaaatc atcgctaagc cgaacatggg caaagtaaac
    15721 atggcaataa tcgtaccaat cgttgtcgta atgaaaccta aaatgacagc atttgggcta
    15781 taaggaaaga aaactggaca atctaaggca gggatcgcat caggaactaa tttcatagct
    15841 atccctcgaa atgccggcac gatttcacct aacagtagac gtacaccagc tagcaataca
    15901 tacacaccca ctacgaattg gatggcttgt aaaaaggcaa acatgatata gttttgatct
    15961 cctgataatt cagaagcgaa tggttcgcct gcaaataaaa cagtgatgat atatagcggg
    16021 accatcacga ccatgacaga caaataagta tcttgcagaa attcgaaatg tgtcggcaac
    16081 ttgatatctt cgatcgactt tttattttcg cctttctcac caaaaagctt agcaacaccg
    16141 gcttcaaata agtacccgat cgtacaaaaa tgacctaaag caatatcatt tgatccagtg
    16201 attttacgaa tgatcggttg cgctactgcc ggcatagcaa tggcgaaaca agctccgata
    16261 aaaccaccga ccaagatcaa tacgatccct cgcagaccgg caaaatagcc aaagactgtc
    16321 gtcatcgttg ccatccataa aattgcttga ccagttaaaa aaatgtactt ccattttgtg
    16381 aaacgagcca aaataatatt gaatacaaaa atggctaaga acgtcagtgc aatatctcta
    16441 cctaaaccca aatcattcat tgcttgacca ttgatagatt caatagaagg gatgatccct
    16501 tgcatatgaa atccttcagt aaagattttc ccgaaataag ttaaacttcc aacgatgatg
    16561 ctcgaaccgg cactcaacac ttgaaatcct aggagtgtct ttaacgtgcc agagatgact
    16621 tgtcctgctg atttcttctg cagtagtaga cccaacatag caatcaatgc aatggtgatc
    16681 gatgcctgag tcaaaatatt ttcgatgata aaattgataa cgcccatgtt tttaccctct
    16741 tttctttttg tatttagata actcccttat cttgcagaac tggggtgatt ttttcttcaa
    16801 tttcattttt tgaaacaata tttttcaaat agatcatcgc cgttttagga tcaactgcga
    16861 acttttcgaa ctgcgaacga aaattttctg ctgtgatgat cacatctggt ttcatggaaa
    16921 ctgcggaaga aatatcacaa tggtccagtt tggcttctac tcctaatttg ttcaaaacat
    16981 cctcagcaga catctgtgct gcaaaactac ttcctaaacc tgcaccgcat acgaataaaa
    17041 tgttcaattt gttcatcttt gttctccccc gtatcattta ttatttctca actactgaaa
    17101 aaagcagttg tttgaaatct tcgatatttg tgcattcaat caatcgatcc aacttctctt
    17161 cgttgttgat caattcaatt agttctttca tgatgttcag atgcgagtag gagtctactg
    17221 ctgccaaaca aaaaataatc tttactggat ccatttcagg attaccgaaa tcgattggtt
    17281 gctcaatcgt agctacgctg actcctaatt tattgacccc atcttctggt cgggcatggg
    17341 ctagtgccat gtgtttgcca atcacgatat acggtccgta atcattcact gcacgaatca
    17401 tcgcttcgac atagcgctct tcgacaatgt tttctttaac caatggtcta gcagctagtt
    17461 caatcgattc ctcccaagta gtggcttttt ctttgatcat gatattgctg tccgtcaaaa
    17521 tatcttttaa cataggctgg atctcccttt tgttgatttc taaattgttt gtttcgaata
    17581 atttttctaa cttgccatag atgattcgat ctactcggcc accactttct ttcatgacat
    17641 ctattatttg agtgaataac tccgtcgatt catgactgtt aggctctaac cgctgtagtt
    17701 cttcatttct tttcaaaaag tcttctataa ccaagatatt cgtctcagtt aaaatcggat
    17761 cgacaactaa aagcggctta tttcgataat tcaattgaaa ggtgctgaaa accagattga
    17821 catccaattt atctactaat tcgatttctt ttgaacttaa aactgcagta atctcgatct
    17881 gaggaaactt ttctttcaga ttctctgcta acagatttcc agtagccatc ccatggttac
    17941 agacaacgac tgatttataa acataattca agtctcgctt gattacactg gcgatcgtcg
    18001 aaaagtggat gacaagaaaa gctatctcgt cctccagtat cttgccacca attacttctt
    18061 cgatcgtagg aacaaaatga gtgactgcgc ggtagattgg gaaataattt gttcggatgt
    18121 tttctttcaa tggattcatg acttggatat gattcttgat tcttacaatc agtgcagcca
    18181 tgtgcttata gagattttcg aataaagctt cttcctttag atggaaaggt acgtgtgtct
    18241 cgttctctac aaattggatc aattggatcg ttagtaattg agcttgtacc cactcgattg
    18301 aattggagat atctctagca ttgaacgtct caatagtgaa acgaagataa tttctttcta
    18361 cttgcgggac attgtccaat tgaaattcct cgatcatgtg gtcaataaat gcagaaaagc
    18421 tactgtcttt tatttctgta tttttccaat taatggcaga gataaattca tgtttcctga
    18481 ttcgctggat ccagatcaat gtaaaaagca ggatttgttt tttataaata tcatcttctt
    18541 gtttcaacaa cttattggaa tagatctcct gtattttttt gatttccacc aaaggaaaat
    18601 aatggagaaa gattttttga gccggtgtta attgtgcatc ttgattcata aagaagtcgg
    18661 tcattcccag attctgattg ataaaatcat agatcatcgt acggatagat cgttctgaac
    18721 ctttataaac cagtccttgc tttccataac tgacgatctc tatcccataa ctgaccagtg
    18781 ttgctcgtaa ccgtctcata tcttcatcta atgtactttt agagatttga aattcttctt
    18841 cttttttgtt tagatggatc gggattcggc tgtaagcaac ggctaaaact agatcaaaaa
    18901 tacgttcgcg ccgattcaaa aagccgtctt tcacatcttt tatttcagtt tctaatcgag
    18961 cttgcttttc cttttccagc ttcaacaaaa aaactttgtt gcgaattgta cggatctccg
    19021 gaagcttttt actgctcaac cagatattcg cttcaagaac ttcctgacgg atcgtccgct
    19081 gactgacatt gaactcactt gctagatgtt ttgcttttac tggatattgg gctgtcatga
    19141 ttctgtgaag tatttcaatt gttcttgttt tcatagaaat gatctccttc tgatttcaag
    19201 tatatggttc cgcttacata ataaaaagta gatatttctg ccacttgaag tggaaagaaa
    19261 gtaagcaaag caaacaaaaa ataagccata tgctttttca tctcctcaac gaaaaagaca
    19321 tacgcctatt attacaaaaa ttttgcccat ctatacaaaa aagaggttgt gaaaaagctg
    19381 tcctgcatca agtaataaga acaactaaac taaaatagtg cttcctattt ttcgtttggt
    19441 tgttcttatt atcgcagatg caagctttta aacaccattt agtggagagg ttgtgacaga
    19501 gatgttcagc cttgagcgat aagaagcgat tttgaaaaat cgcttcccat attttacaaa
    19561 atcgtgcctt attgccgata ggctatctct ggcacactgt ttattcagaa aaaggagctg
    19621 cgacaaggct tttgtcacaa ctccgttcat aaatatttcc tgtcaatcca gcataatcgg
    19681 ctgaataaaa tcggcttgcc atcttttttc tttctcagaa aaatcaaaat accatatctg
    19741 agaaaaatac tgtcgttgtg tcttacttcc tgagatcaat cgcccatctt ttcgatcgac
    19801 tgaataatca cagcatctga aatcaattcg gatggcaaaa ctattttcgt ggatctgtcg
    19861 ataatttcct acttcctgaa tagttatttt tttagtatga tttcttacac cgtctgcttt
    19921 gtttttttgc agaactttct gatgttcggc aaacagcttg tctgtataac agcttctggc
    19981 tttttcagga gtttcttctt cccaagcgga ttggatcttc atgaatatct tttggatttc
    20041 cagaaactgt gctttctttt ctttagccgt tcctgacatg cgggacaata tctccgcttt
    20101 cagccttttc ttttgtcttt tattcttgac gatagaaaaa gcagaaaccc ctcctactga
    20161 caaaacagct aatgcaagaa atcccgtttg ctcataacta gaaggacctg tacctggacc
    20221 ataataccca taagaatctc tgccgtaaga acgactgcca aaataactgc ccccatatga
    20281 acgaccacca aatgaacctc tgcccgaact tcgaccgcta gatgtacctc caccatgacc
    20341 acccgcgaca gcgaatcctt gggtcggcag agcaaacaaa cataggccca ctaacaaaat
    20401 gattgctaaa taattgattt ttttcattgt cttcctcgtt atcttttatt acaaaaaact
    20461 atttttatga tagccttgct aaaaggaaga gtcaataacc agatgcataa actgtttttt
    20521 tagcaatttt gtcttattta gtcattgagt aacatcggac aaatgtatgt gtcggcagtc
    20581 cataattcct ctatcttcag catgaacaag cttgcagaca agagatctgc gcttcctcca
    20641 ggagagattt ggttttcctg acaaaagtgc aacagtgctg taaatggacg atttggtaaa
    20701 gcactttgac tagcagcatg agcaagtttt tgcatttcca ctaacgtttt ctgatcactg
    20761 cgatgcaata cgcatgtgtc atctagcgaa ctcatcaaaa ctaacaacat atgcatttgt
    20821 ttctgtacat atgaatcatt atctagctcc ttagctgctt ggattgcaag cctgatatgc
    20881 ggatacccta atatcgcttc ttcatatgca ccatttacgc catatttttg cttcacccta
    20941 tgaccatgag tggttttttt atgacgatat tttgtatcag gaaaagcagc aagcttgctg
    21001 acatcatcaa gtattttcga taaagaaaaa ggctgttcca tactgatttt ttgtgcaatg
    21061 acactgatca atagccccat tgcccaaatc gctcctttat gggtattgat gccatttgta
    21121 gcaatcagca ttgccttttc cgcttttcgc ccaatctcag caattctttc tcttaactcc
    21181 tgatcaatag catgatgcca tgctatttga gccatttctt caaaaaaagg agttagcgtc
    21241 ttagcagact taagaaataa agcatagtcc atatcggaat gtgcacccgc atccagtgca
    21301 tccactaatc caggttttgg acttaatgtt acttctgcga tcaatgcttc ctctaccaac
    21361 gcagcgagtt gctgagtcac tgcttttttt cgttcgtcat ttatcatgac actcactcct
    21421 tcttcctact attacttgct gaaacctttt ttgatagcta cccaaatgat atgaagtaac
    21481 cagcttaaag caaatgcaca acttgccgtc attagataag ctacaagaat catgactcct
    21541 gaaggtaact gcagccaact atctttaaac aataataaca gtacttggaa aacaaataca
    21601 ttggctaaat acgtacgata agcataaaca gacaaccatt tgaacaatgg caattttggc
    21661 gaatcgttta caatcatttt ggaagcgcct atgaagacag caaagatgat gactaaactg
    21721 tataaacttt ggatcgtatt caagtaaggt gcattcgcaa agctcaagtc tcctggatgt
    21781 gcgagcaatg aacggacaga aaagaacgcg ataactaagg caacaggtaa gaaaagataa
    21841 cgtactctgt ccaaatattg atagactttt tcgtgataaa caaatgcggc taccccataa
    21901 atcatgaaac tagcaacaaa ccgatctaat aagtaccagc tgtcatgata aggcccagtc
    21961 aatacttgtg tttgatagaa cagataccat gcgacatata aaatcgttga accaatcaat
    22021 actggccata catatttttt attagcaaat actttgtaac cgagccaaac aaaaaacggc
    22081 atgaacaatt ggatctgcag catcataacg gtataccata aatgaggcgc tccatcacct
    22141 gtgacatatt tcagtaaaaa gcttgcagta tccgtatacg gagtttgttg ttggactgct
    22201 ggaaatagcc aaagataagc cgtcgtccat agaagatacg gaacgactag ttcaaaaaac
    22261 ttttctttca aaaattccca ataagacaaa tgctgacttg tttttaccat attataaaca
    22321 attgcaaaaa taaacattgg tgctgtgtat ttaacgatga cgtaaaaggt accaagaaaa
    22381 gctgtatttg ttgcgctgtg gctgtttgcc aaagcaaagc tcaatatcgt ttgcatcata
    22441 acagccgtta cagctgatac tttctggaag tcacttacgt tgattttgat ttttttgttt
    22501 tcaaacatct ctttctctct ctttttcttt attaccaaag ttctttggtt aatttaggtc
    22561 cttcgactgt tttcaataga atttttttct ctggctctct caaatactct tttagtgaaa
    22621 aaccgccttg ccgagtcact acttgggtat cgaattgctc aaaaaaagca ggatgcgatt
    22681 gaattacctg aattggcaat tcaatcggtg aatttgcgta cagcagcaaa tcaaaatcac
    22741 tggtttcttt tactgccgga atcccagtcg ataattcaaa tcctaagctg ccgccgattc
    22801 cccatttaca tcctgataat atgtttctag cagcatgaaa ttgctgataa acaggataat
    22861 ccatgatttt ctcttgtcta tacgattctt gttcgatgat gtcccacgga tgaatgactt
    22921 gtttgacggc atgttttgag atttctgttg accatctctg attgcgggca ggtccacgta
    22981 aaccaatcgg tagtatttct gcgtgatccg tcgacgcacg acgaactgtt ccgaacatct
    23041 tttcagttag aacgacccat tcaggcaagg tgaacccttc tatttcatgt ttatcaaaaa
    23101 ggacaatatc atgtgcttga atcatttttt atgcatccca ttcttcgtcc attttggcac
    23161 ggactttatt tgtttcgaca cgtccgaatg ttttggctgt ttcgtttgtg tatcgatttg
    23221 tcagcatcgt gtcatttgta ttgcggacat cttgaatggc ttcattgatt gcttcgatga
    23281 ctttgctgac attttgttct gaagcagctt ggtcggtgat atcttctagg aaatgataga
    23341 tcgctcctag ttttgtataa ttattgatat cgtaagcgat agctggaact ttttcagcag
    23401 ctgcttcgat ttcagcaacc gaacgttttg tcacacgtgc agcactttct ttagacatcg
    23461 cttggacggt aatttcttta ttgtttaaag caatcaagcg gcttgattgt aatccatgtg
    23521 ctaaaaatgc acctgaaata gcatttcctg caatcaatcc aataactgga tgtccagctt
    23581 gacggagttt tgcataagca gcagcactat tagccaaagc cacatggata ccgatcaatt
    23641 cttcgttata gccgtatgct tggcttggta catcgatcac aacgatgatt ggccgtttca
    23701 cttttttgtc tttatcttct tcgtaaacgt gatccaaaat ttgtcccatg cggaaacctt
    23761 caactaaacc aacttcaccg tttcgtacac ggtacatcgg gtttttagga tcaggtacga
    23821 ttgatgtcac gatggcttct tctatgaatt tacttgattt accataatac acagtactaa
    23881 tagaagaagt tggatttttc atgccagtta gtgccgcaaa ccaatcgtaa ccaacacttg
    23941 ctgcagctgg ttcatttcct tctgctactt ctggtaagtc aattgatttt gcttgatggt
    24001 tagcaattgc ttcattgtaa tcgtcaatgg ccattggttt tgaaagatct aaagaatcaa
    24061 gtaatgataa ataaaatgct gagctttcac tgcgatgcgt gtcttttcgt tcgtccattg
    24121 ctgcgatgac agcagctttg attgcttcta cactatcagt cactaattca tcaatgattt
    24181 ttgattctac ccgttgacga gatcctaatg tattccaaat caagtctttg tcactagaat
    24241 caaattctcg aacgcctgct tcttgttcaa taacttctgg tccattcaaa ccgactctcg
    24301 ctttttttgt tgcgattaaa taactcatta atgctgaagt aatcgacata ccgccaaatg
    24361 atcctacacg tcctggaatc aaaccgatga ctggtacata ttgttttaga gcaatgatta
    24421 catttccgat ttctgaaata gataacagtc cgtagtttgc ttcttgcaaa cgaacaccac
    24481 ctgtatctag tacaatgact ggaaagatct catttccttt tttattttct tctaacacat
    24541 gttcaagtgc agcaacgatt tttgcaccgg aaacttcacc gataccgcca ccttggaatt
    24601 ttccttccat agcgattact actgcttgtt tgccattgat cgtgccacgg ctgacaacga
    24661 tcccgtcatc actttcaggg acgattcctt gtgctggtaa acttggcgcg atgatttgat
    24721 cgaatggatc aatcaattca cgtccattgt caagtagagc gttcactcgt tctctggcgt
    24781 ttaattctac aaaactattt ttcatctgaa agttcctcca ttgcttgcgt caatcgtagg
    24841 tacacaactc ctggtgtagc accgaagtca ttgatcacga tatctgctga aattggataa
    24901 cgatcaaaaa atctcgttaa tacattgttc catacttcgc cgaagccatc gcttcctgta
    24961 caaattttta cagtagattc tgttttttct gttggaaaca tcaagatttc caaatctcct
    25021 gaaccgacca ctcctacatg gattttctta ttgatcggct gtttcgttgg atatttataa
    25081 gctaattgtt ccatggatct ctcccctttt tgtaaaacag agcatacacg ttctgcttat
    25141 tcgtctttct tcttccatct gggtaaatgg cgacaaaagg tttttctcgc caattttatg
    25201 accaagttct gaatttcatt ggtggttcat acaggccatc tgaccattcg atcaaatcat
    25261 cgatggtttg tgcagctaat aatgaacgtt tcgcttcact acgtttgatg cctagatctt
    25321 ctggtaattt cacgatgccg gctgcacgca agctatctag tgattctttc gtgctagtca
    25381 ttccaagagg tgtaacaccg ccaattgctg caattgctgc ttgtcgttct tcttttgttt
    25441 ttgctttgta caaataagca attccttctt ctgtcacgat atgtgtagta tcttcgctat
    25501 aaatcatcac tggtgcattg gctaagcctg cttgtttttg aacagctagt gcatctaatt
    25561 cttcgacaaa gactggtttt ttatttgaac catatgtttc aaccatttga acaactaatt
    25621 tttggccttt ccctaatgga tcatcattgt cgcgaagtga ttgccatgct ggtgtagaat
    25681 gacgacgacc gccaggatta tgtcccatgt taggcgcacc gccaaaacca gataaacgtc
    25741 cttttgttac ggtagaagag tttccttcgt aatctaattg caaggttgaa ccgataaaca
    25801 tatcgatggc atattgtcct gcagcttgtg ataatgttcg gttactgcgc atcgtgccgt
    25861 ctttaccgac aaaaaagaca tctgggcgag cagcaatata tttttccata ccaacttcgc
    25921 caccaaaact atggatgctt tcaacccaac ctgattcgat ggctggaatc atcgttggat
    25981 gtgggttcaa tacccagttc ttcgcgattt tcccttttaa ccctagtgat tcaccataag
    26041 ttggcagtaa caattcgata gctgctgtat tgaatccaat accgtgattc aaagattgta
    26101 cgccgtgttc tgcgtagatt cctttgattg ccatcattgc cattagaatt tgcaattctg
    26161 tgatgttttg aggatctcgt gtgaataaag cttctaattg ataaggttta tctgctttca
    26221 cgacaacgtc tacccagtca ccagggatat ctacacgtgg taaagaatcg acaacttcgt
    26281 tgacttgaac gatcacgata ccatctttga aagctgcagc ttcgatgatt gttggtgttt
    26341 cttccgtatt gaaacctgtg tacaagttgc cgtctttatc tgctttatct gcagctacta
    26401 aaacaacatt tggaatcaaa tcgatgaata agcgtccata aagttccaaa taagtatgga
    26461 tatcacctaa tttcattgtg ccgtcttcaa tcatttgggc gacacgcaag ctttgtgcac
    26521 cagcataaga gaaatcgatc ttgcttgcga ttcctttttc aaaaatattc aaatgttctg
    26581 gtcgtgagat actagacatg atcaaatgca gatcattcac tttttctgca tctacttttt
    26641 caagactttc tgaaagaaaa cttgcttgtt tttgattgtc gccttctaat acaactttgt
    26701 cgccgggagc aatcagtttt tccattactt ctacgatttt ttcagtttct acatacttgc
    26761 cattgagcaa gttgctgatt ttttccaatc gctgcgcttt tttttcgcgt ttagaagccc
    26821 agttgcggtt tggtttttcc ataattagtc atctccattt ttgatataga aacgtggcgt
    26881 catcaatacc atgttggtct agattgatcg tacggatccc tttatctaca ccaaattttg
    26941 tatgaatcag ctttgtcaaa gtattgccgg ggggaaattc aatcgtcaga tccattccta
    27001 attcttttgc caagtccatc atttggttcc actgcacagg gaaagctagg ttttccaaca
    27061 aatctttacg aatctcttct gcgtcctgag tggctcttcc atccgtattt tttatataca
    27121 aacatctagc atcagataga tggacagact gcatatacgg cttgaatttc ttgaccgtat
    27181 cattcatcaa gagacagtga gatggcacag ggactttcag cagcttcgct gtttgcgctt
    27241 ggtgccgttt ggcttctgcc aacgtaacgg ataaaccttc gatactccca gataacgtat
    27301 gttgcatcgg acaattttca tttgacagat aaactggatg gtcaggatca aatgtttttg
    27361 ctacaatttc ctcagcttcg cttctcgtca cacctactac tacgcccatt gcataaccgc
    27421 ttggataagc ttctttcatt aagactgcgc ggtgatagac tagttccgct gcattctcaa
    27481 atctcaaaga tcgacaagca acagctgcac aaaaagcacc aatcgaatgt cctgctgcaa
    27541 tatcaggctg aataccgttt ttttccattt catctaaata aaatactgct tttgtcagta
    27601 atgctagctg gataaaaacc gtatcttgat agttttcttc tttatcaact aaccgatacc
    27661 ccgtcacatt ttctaaacgt tccatgtaca tttcagggat atccgctaac atccctaact
    27721 tctgactgcc ttgtccagga aataagaaac ctgtactcat gagcctcgcc tcctcagcac
    27781 ttactttttc cactgctatc ttagtcaatt tctgctataa taaaaaatgc caaaaaaaga
    27841 aaaaaccata acaaaaaggt atggatggga tggataatta tgaaattgct tcatctgcag
    27901 tattttaaaa aagtattcga aacaaaaaat gttacccaag cagccaaaga attatttatt
    27961 tctcaacctg ccttgagccg tgcgatcaaa catttggaaa acgaattagg tgtccctttg
    28021 ttttaccata gcggcagaaa cattgagacg accgtttatg cagaagaatt ttatccatat
    28081 gcagtcaaga cattagacac attagaagaa ggaatcaaat tattgtcttc ggtcaatgag
    28141 aagatcgtta cttccgtcat attgtattta gaggtagcct ctgtttctat tccgaacttg
    28201 gtccgaattt ttatggaaaa gcatcctgat atccaattaa cgatcatgca acatcattta
    28261 ccagatgata ttaccgatcc tgtactgtac atcacttcgg agatgaagcc tggcatgacc
    28321 aatattccta tcatgaaaga acctgtttat gtcgccattc ctaagaaaca tcctcttgct
    28381 aaaaaagaac atctgcaatt agaagatatc caacgcaccc ccatcttgat gctgtcaaag
    28441 agcaacgcct tccgtcatac actggatgca gcacttgaag cgaaaagtat cgaattgacg
    28501 atcagttcga cgacagatga tcctgcgaca ttacgttcta ttgtcaagca aggattagct
    28561 gttagctttt tcccaaaagt ttcttggtct tacgataaaa atgatccttt cgtcttgcgg
    28621 gaaatcaaag atttgccctt gacacgaaca atttatttat cttctagctt cacagaagat
    28681 catcctttga tcaaaatgat tgcaaaaacg atgcgggaat tttatttagg aaaataaaaa
    28741 aaaaagagct gggacaaaat tcttgtcgca gctctttttt cgaacaaacg gtgttccaga
    28801 agtaatttct tagaaaataa gccgaaattt cccaaaaatt tgagaagcaa ttttcgtaaa
    28861 ttccttctta tttcttgaag ctaaacattt ctgtcacaac ctcttctcta ttgtcctaga
    28921 cattcattgc gttgatttaa aacttccatc aatcaaatct gctagttcat ctagcttttc
    28981 tattttacgt agatcaatag agatccaatg ttctttattc atgtgatagg ctggaaaata
    29041 cccctctttt cctcgcaaaa ttccgccaag ttcaggatcg accttcaaat caagaatatt
    29101 gatgatccta tggtcacttg catcatcata gattttttct tctggaacag acatcaggac
    29161 cccaaaccat tttttgcttt tttgatgtct gaatacgcaa taatcaggaa attttttaaa
    29221 aatatattct ggagacaaat tgtatgtttc attgatgtga tcgattagtt cttttttcgt
    29281 tagcatctgc ttccatctct ttcttattga ttcgctttat tcaaagatac cacgtaagaa
    29341 tagaaaaatg aatacaagta gttgctaatc aaatacttcg atatttttct tttttatcaa
    29401 ctcttaaatg ttcaatccgc ctgtgttggt cttgctacca tctactatta aaatgtttat
    29461 tattgtttgc ccacctattt tttgatttta gtggtatcat ttttcaagtt catgatgaaa
    29521 ttcttatcat cagtaaatgc tccagaaaaa taaacaatga ttgaaaaatc agttgaaact
    29581 tgaccaccgt ttattttctc cgtattctta gaatagaagg gagtctgatt gatgtattca
    29641 ctaatgaaaa aaatcattac cgaaaaagat attcgacgtc atgtttcttt agtagaacaa
    29701 ttgctgaatc atacaaagat aaccgtaaat gaattagctg aaatcatagg gacaaccgaa
    29761 cgaacgattt tttctgacct tcaatctatt cgctctcagc tgcccgaagg ctgggacatc
    29821 atcagcgatc aagcaggaat cagtttgcaa aaccagcaaa atttattaac aaatgacctt
    29881 tgggagattt ttttcaaaca atcagtcagt gtcgaactgc tgaaaaatct gctttttaca
    29941 aaaaaagttg ctgtccctga ttttctagcc gaccatgggc tttcttatgg gacattgaaa
    30001 agacatgtga ctaaaatcaa tcagcgttta gcaagttacg acttgcagat cgatttgacg
    30061 aaatatacag cttgtattct tggaaaagag cgtgcgattc gtacttttta tcatcgattg
    30121 ctgattcctt tcactcataa caactatttt ttcgaggatt attcgatcca tgaatcccat
    30181 tattttcaat ttttaagaaa cctcagtcaa actgaactag cggttgaaac agaagagatt
    30241 ttcggaacat gttggttctt tatcaatacg atccgtataa aagcgaattg ccgattagat
    30301 tcttctatcc ataccaacag cacactctcc tccctgtatg actctgcatt gaaaaaattg
    30361 tatttaacgg aaggtattta tcttaaagac actgagcttt cttttgcttc tttctgcttt
    30421 ctggaaagtt ggaattacaa caataactat ggacaagaaa ccgctcgctg cctgcatcat
    30481 tctccatttt tagaggtcct cgaaacgttt gtagaggaac tagcttcaga gctgtctctt
    30541 gatcaactaa aaaaaacatc tctgactgat aacttggctc tcctcatatt aaaatatcat
    30601 gaatcgccga tattgattga agcattggac cgtcaatacc atcgtttttt agaaacatat
    30661 gatcagcaat atgcacactt gtatcaacag aaagagaatt tactgaacga tctaaaaaaa
    30721 gaaatagaaa tcaatgaacc aaattacttt ctccaactct tgtctctact gatccaacaa
    30781 acgatttttt ctatcaaacc gaatttgttc aatgtctatt ttattttcca aggagaacct
    30841 tcatggaagg cgttcttgca gcaggaatta aaagattact taggaaaacg tgtctgcttc
    30901 ttacctatcg aactaaccga tctttccact atttcttttg aaaaaacaga tatcttgatt
    30961 tccaatttcc ctctagattc tctagatatc cctattgtat acatttcttc tattccaacg
    31021 aaaaatgagc tcaatcgttt gactgaactc actttcaaaa cttttttata gtaacggcta
    31081 gcgactgtat tctctagctg ttttttcttt tttgcgaaac agtttgttca tgatcccttt
    31141 ttctatttct acaactaaga atagaagaat cacatttgct ccaatcaata actgttgtgt
    31201 catcgttaaa cttactgtac cgatcagctg ctggccgact ggtagaaaaa taaccatccc
    31261 ttgcaataaa gcaaggatac ctaatgaaag aaaaagcgct ttgttttgca aaagaccttg
    31321 gttcaatgac ggcttggaca actctctgca attgatcatg taaacagctt gagctaagac
    31381 gatattttgc agaagaatgg tttgctgtaa gctttgccct tcaaactgca tagccaataa
    31441 ataggcagga accatgatca gtagggagac atagatgatc cgaaacaaat gataaccaga
    31501 taggattcct tgctttggat ctcttggtgg gcgcttcatg atttctggat ctggcgactc
    31561 gaaccctagt gcataagata aagtgattgt cgttaccata ttgacccata aaatctgtac
    31621 tggggacaaa ggtagcgggc gattcagcat aagtgcccaa atcaagatca atccttgagc
    31681 taatgcagtt ggcaagaaga aattgatggt tttttgcaaa ttatccaaaa tccgccggcc
    31741 ctcttttacg gcttttgcaa tcgtatggaa attatcatct cctaaaacca tatcagctgc
    31801 ctgtttgctt acttcactac ctttgattcc catagcaatt cctacatcgg atcttttcaa
    31861 agcaggtgca tcatttacgc cgtctccggt cattccgacg atttctccat ttttctgcaa
    31921 agctttaaca attcgcagtt tatgttctgg tgtcgttcga gcaaagacat cgactattgg
    31981 cacttgctga atcaactctt catcagacat aagatccaaa tcgatccctt ctaaaacttt
    32041 ggcagtatgt tttaagccaa tttgttccgc aatcgctttt gctgtgtctt tgtgatcccc
    32101 tgtaatcatt ttcactgaga tccctgcttg ttggcattcc tttacagctt tgactgcact
    32161 ttcttttggc ggatcgatca atccggcgat ccctgcaaaa gttaattggg ttaaagtctc
    32221 atgcgtgagt tcctgtttcg aatctacttt tttataagca aatcctaaaa cacgctgacc
    32281 ttttctcgca aattctgcag ctgtctgact ccatgcttct tgttctgcag aagaaagagt
    32341 tgatttagca aataatactt ctggtgcacc tttgacgtaa atcaccgctc ctttttcctc
    32401 cgcatgcatc gttgccatat atttgtattc agaactgaac ggaatctttg cttctatttt
    32461 tttcaaaggt agattggctg tttctgtgtg tcgaatcagc gcaacttctg tcggattgcc
    32521 acttaaattt tctgttttct gttcttcttt tgtttttaat tcctgacagt ttttcataat
    32581 ggacaacacg caggcttctt cttttgcagc tacatccaca actgtcattt cattttttgt
    32641 caatgttcct gtcttatctg aacagatcac tgtcatagaa cctaatgttt ctaccgaagg
    32701 aagtcctttg atgatcgcct tttcttttgc catttcatgt actccaacgg atagaatcat
    32761 ggtcaaaaca gctggcaatc cttcgggtac cattgacacc accaaagcaa tcgtagccga
    32821 aagaaggata tgccaatcca taccatatcg aaatgtcgta aaaataacta agaataggct
    32881 caaacaaaga atgccttgaa aaatttgttt gtttaatcgg ttgatttttt taatcagagg
    32941 cgtcgtttgt tgttcgaccg attgcaatgc ttgattgatt ttaccgattt ctgtcgtgtt
    33001 tcctgtttct acaacgacac cgactgcaga accagattgt acaagcgttc ctgaaaaagc
    33061 catatttttc tggtcgccga tcgattcttc ttctgtcaaa acggcatgac ttttttcaga
    33121 tgcttctgat tctcccgtca aaatcgattc ttccactaat aaatcatgtg cttcgattaa
    33181 tcggatatct gctggaacga catctccggc acgtaagaca atgacatctc cttttaccag
    33241 ttctttcgca gggatttttt cttgcatccc gtttgatagt actatcgctt cttgtcccat
    33301 cagctgcttc aaaccattca atgattcttc cgcttttctt tcttgccagt aactaacgag
    33361 tccattgacg atcactacga ataaaataat acttccttca atatattcac tagaagcaaa
    33421 tttcaaaaaa gaggcaatga tcaacacgat catcagcaga tcagtaaaat gctttgctat
    33481 ttttttccat cgttttgtct gctgccttgc ttttatttca ttatatcctt gatcagtcag
    33541 gcgtttctgt cgttcttctt gcgacaagcc ttctgttgat gtttgatagt ttttcaatac
    33601 atcttctacc gtcaatctgt accagttcat cctatcccct cctgtttaat tgatactgtt
    33661 attatacgca gggaagtttt ttcaggcgta tcgattattt caggggggtc tgaaataatt
    33721 cgttttgcaa aatgtatctg ggcctaactg atatttctat tgcttattct tttatataca
    33781 aaatttaaga atacaaacca gataaccaat aaaaaaggaa taaagcgtgt cgtgctttcg
    33841 aataacaagc tgaaagcaat tgaagtaaaa aacaccaaca aagcaagatc tttatatctt
    33901 tgtgtagtgt tccgtttctt ttctttaata aattgaacat gtgcacacaa gatcgaagac
    33961 cacacaaata gaaaacaact cgttgtcatg cctgccaaaa gctgaaaaaa caatcgacta
    34021 tcagctacaa aaaaatgtag acaaagaccg gcaaaaaata acattcctgt aattaaaata
    34081 ccgtgtaaag gtacgccttt cttagaaagc gcacaaaacc aatggggcaa acttttttct
    34141 tttgctgttt gatataaaat cctgctagta ctgtagactg cactatttgc tgaggacaaa
    34201 gaagcaacca aaatcattag attgacaatt gctgctccgc cttggcaatg gatcacttgc
    34261 agcatagtca ctaaaggact ttcttttaaa tctaaacaat gccatggaac aacaaataaa
    34321 atcgacaaaa tcgttcctat atagaaaaag agaatacgcc aaggcagaca gttgattgct
    34381 tttttcaaat tcttttgcgg atcacttgtc tcgccaacgg ttaagccgat catttcaatt
    34441 ccagcaaaac tgaataaaac cataggaaat gagactacca ttccagcaaa gccaaacggt
    34501 agtaattcat cgatctgtcc aaaattttct ttgattccga tagactgggt atgaaaatct
    34561 gagacactca atcctatacc caataagata aaaaggaaaa tccccaagat cttgatgatt
    34621 gagaaaaaag attccaattc accaaaccac ttgactgcaa caaggttgac tgccattaag
    34681 atcattagtg cgctacctcc gcttatcaaa gctggtacat gtacaaacca gaaatggata
    34741 tacaatccta ttgctgttaa ttccgcaagt ccagctccaa gccagcaaag ccaataagtc
    34801 caattgacaa aaaatgccca ctttttgcct aaataatagt caacgaaatc actgatagag
    34861 ttaaatgtac tatttgctaa aaataaatca cccatggccc gcatcaaacc ataaatagcc
    34921 aaaccgcata tcaaataaac gagaataaca gctggccctg ctgatgcaat tgcctttgct
    34981 gaacctaaaa ataatcctgt tccaattgcg ccacctaatg cgattaactg gatatttcga
    35041 ttagttagag tccgtttcat tgccggtttt tccatcaagt tcaccttttt ctttttcaag
    35101 cagcatggcg acttcctgtt cccaagaagg tttctttatt ttttcaatac agagtggtac
    35161 tacaaacaaa atgacactga tggctgtgac tgcaattaga ttgatccatg ttccagcttt
    35221 catcgtcagt ccgacagaaa atccgattgc cgcaaataaa acaataacga cagtccaaac
    35281 tccaagattg ctctttttct ctttttttcc aattctaaat ggtcgattca actttgcatg
    35341 tgtataacgc aatctcaaaa ctgcgatccc catgatgaca tagacgatac agtagataac
    35401 agtagttgaa tttacgagca tcaaaaatgc ttgattgacg ccaggaatca gcaaataaaa
    35461 aagtgcaaag attgagataa tgacagactg agtcaaaata acggatttgg atacgtcaaa
    35521 ttgattcgtc ttccaaaaat tgaattttgg tggatacgca ccttttctag cactttctgt
    35581 cactgtcttt gctggtcctg aggcccaagc agataattgg actgctacac caatgaagac
    35641 aagtaaacta aacagattca caataagatg tggcaaacca agaatttggc aataaatcga
    35701 aatagattgc gcgatattat tcaattccat ttgtcctttt ggtacgacat ttgctacaac
    35761 taaagcattc aaaatattga cgaaaaatat aaaaattaac gcggcaaata ctccgcgaat
    35821 atacgtttta actggatttt ccaatcgagt gatatacaca gaggacattt cgataccagt
    35881 aaaaataaac atgatcggcg caaaataaac aaatgaggat gcagtcgtag tatctggtat
    35941 taatttgtcc catgaaaaac tgcctaatgt tccggttggt acgatcccaa ctttgatcca
    36001 cgcagcaaac cctaataaga gcatcatcgt caaaggcacg tacaatccta accaaacacc
    36061 gacttttccg ccgatttttg ccatatcaaa tttcaaattc aaaaaagtga tgatccaata
    36121 aacgactaaa atgacaacta atgtaaattt gctgttcaaa ccaagcggca cattatcgat
    36181 aatattacca aacaacggag cgagtgctga agcgaccatc accatcccag gaaacatttg
    36241 gacccataac agccatgaga caacgaaccc ccattttttt cctagagcat tcgataccca
    36301 aagttctgga ccaccttttt ggggaaacat tccagcaaat tcaccagaga tcaatgagat
    36361 tggaaaagcg tacaataata ctgcaaccag catgtaaaaa atcatcgtcc agctcgttgc
    36421 tgctacatct ggaatatttc gtacacttgc tacaagggcg caggtcattc cgataaaggt
    36481 catggtcgtg atttgctttg atgaaccagt atctttcatc tttattcctt ctttcaatag
    36541 cgacatatgt ttttgttttc ttttattttt gttcaagaac agaaatgaca aggtttcatc
    36601 gattcagtac aaaacccagt atcaccgaaa catccattga acgatcttag tgaactgggt
    36661 ttgatacatg aaacgaacga tccaacttat agaacgtttt ataaaaaaga atatcttggc
    36721 gaggaatata tgaaaaacag ggggaaatac aattgttttg atgaagcaaa taattgtctg
    36781 ttctccatat gtctcttgtt attctttcac acaaaaatca gttctattgg atgatttcgt
    36841 ttagtttctt ttccattgct ttggcgattt tttttgtata aaaattgaaa attcgatcat
    36901 cattcaaata aggagtcatg atagccgcac gtagcagagt gattttctgt tcttttttcc
    36961 attcttcttt cgttagaccc attctttcaa caaaacgaat cggagaatca ccgtaatctt
    37021 cttgtgtaaa tgtagtatga gaagtaataa accgttcacc ataaacatcg ccatccatat
    37081 aagaagaccg atcgaacatt ttttcattta attcattgat agcgttcaaa tctgtacagc
    37141 cttgttcttt gaatacccaa ttgaccatat taaaatcagg atgattcaat ggatagactt
    37201 cgattgtttt tcctttaaca gtaaaagtca gatgatcgag aaattctctg aatctttgag
    37261 ctgcctcgat tgaagcccca atcaattgac catatccggt cacattcaat ggcaatacac
    37321 gatgtgctgt ccagactgcg gcggcggttg ctccagcttt tgatccttcc aaaatataag
    37381 ctcctaacat atctggagcc tttactgatt tttcaaacac atatggcgcg aaataagaaa
    37441 tgatatttct catattttta tgtttgatta cgattcctcc agcagcataa gggacatagc
    37501 ccatcttatg aggatctatc gtgacagaat ccgcttctga aatagctctg aacccttcat
    37561 atacctcttt gtcaatcgtt acgcagtggt ggaaaacatg atgttcttca aagaattcag
    37621 ctaaggaagc atacggcaca aattctcctg cttcgtttag aaacagtgaa cgcgcatagc
    37681 cgccatatgc ggcatcaaca tgtaaataga aatagatccc ttcatctttt aacctttctc
    37741 ttaattggac gattttatct acactgtcta cttgaccttc ttctgtcgtt cctacgacag
    37801 caaccacacc tagaattggg attttttgtc cagctaattc acgaattgtt ttttccaaag
    37861 cattaatatc cattcgataa tcctcttgaa ctgggatagc aaccatctga tctaagccga
    37921 ctccgcaaat atctaacgcc ttcatccaag aatagtgttt tgtttgaggg accagccatt
    37981 tccctaatct ttggatattt tttccgctac gggaagaagc tgctttgacc tcatccattt
    38041 cttcgtccgt aaagctttcg gtcatttcca aaatttcttc aacagataaa ttcaaaagtt
    38101 cccactcaga catttttttc actttttcag gcagcacttc cttgacagct aaaggaatcg
    38161 atttgatgca acgggcgtac cataatcctt caagattagc aatagaacca tctgctgcaa
    38221 tgtgtcccca tccgtctgtc atattgaata atgaagcaaa atcctgtcct acttctgctt
    38281 ccatttgcga agttgccata gaagattcca atgcaacatt atttgggttc cacagcatcg
    38341 catagttgta agctaataaa gcaggcatca atgtttcagc attcatttgt ccccagtaac
    38401 ggcctgctga atgccatggg atcgatcctg cgcgtaatcg ctgactgact tcatccaaga
    38461 cctctatcat atggtcgcgg gtggctagat aatctggagt gagcttatct ccttcgctaa
    38521 ttgcaggcat atctgaagga atataatttt ctctccatcc aagatgttca tctaccatct
    38581 tgttcaacaa cattttataa actggtccat tttcagcttt gtcgcctata aagacggcct
    38641 tgatatccat atctttcatt tttcttccct ccatattttg ttggtcaatg tcttgcagat
    38701 acatgtgttt atctgtaaca cctcttcact atagcgccaa aaatgagaaa ttttttttta
    38761 taacatcaaa tccacgtaga tttttacaaa caaaaataaa taaagtcgga tttttctgat
    38821 aattcaacgc aaaaaagcga acaatttttc aataaatcag tcttctaaaa tagataacct
    38881 aaaaaaagcg aacaaacaaa taaaatga
//
LOCUS       DAZKCY010000015        35986 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000015 DAZKCY010000000
VERSION     DAZKCY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.015"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACG8XC_000684"
     CDS             complement(38..1189)
                     /locus_tag="ACG8XC_000684"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9190404.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACG8XC_000685"
     CDS             complement(1202..1639)
                     /locus_tag="ACG8XC_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9190405.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACG8XC_000686"
     CDS             complement(1654..3420)
                     /locus_tag="ACG8XC_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFP9190406.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACG8XC_000687"
     CDS             3684..4361
                     /locus_tag="ACG8XC_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9190407.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACG8XC_000688"
     CDS             4364..5491
                     /locus_tag="ACG8XC_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9190408.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACG8XC_000689"
     CDS             complement(5691..6131)
                     /locus_tag="ACG8XC_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9190409.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACG8XC_000690"
     CDS             complement(6142..8175)
                     /locus_tag="ACG8XC_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFP9190410.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACG8XC_000691"
     CDS             complement(8177..9880)
                     /locus_tag="ACG8XC_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9190411.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG8XC_000692"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG8XC_000692"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFP9190412.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACG8XC_000693"
     CDS             complement(10963..11988)
                     /locus_tag="ACG8XC_000693"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9190413.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACG8XC_000694"
     CDS             complement(12197..13066)
                     /locus_tag="ACG8XC_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9190414.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13770..15662)
                     /locus_tag="ACG8XC_000695"
     CDS             complement(13770..15662)
                     /locus_tag="ACG8XC_000695"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190415.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15659..17386)
                     /locus_tag="ACG8XC_000696"
     CDS             complement(15659..17386)
                     /locus_tag="ACG8XC_000696"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190416.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17402..18052)
                     /locus_tag="ACG8XC_000697"
     CDS             complement(17402..18052)
                     /locus_tag="ACG8XC_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFP9190417.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRVSRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18256..18552
                     /locus_tag="ACG8XC_000698"
     CDS             18256..18552
                     /locus_tag="ACG8XC_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9190418.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18744..19364
                     /locus_tag="ACG8XC_000699"
     CDS             18744..19364
                     /locus_tag="ACG8XC_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9190419.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19456..20622)
                     /locus_tag="ACG8XC_000700"
     CDS             complement(19456..20622)
                     /locus_tag="ACG8XC_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9190420.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20634..20987)
                     /locus_tag="ACG8XC_000701"
     CDS             complement(20634..20987)
                     /locus_tag="ACG8XC_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9190421.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            21115..22137
                     /locus_tag="ACG8XC_000702"
     CDS             21115..22137
                     /locus_tag="ACG8XC_000702"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFP9190422.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            22156..22317
                     /locus_tag="ACG8XC_000703"
     CDS             22156..22317
                     /locus_tag="ACG8XC_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190423.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22576..23010)
                     /locus_tag="ACG8XC_000704"
     CDS             complement(22576..23010)
                     /locus_tag="ACG8XC_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190424.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(23120..24979)
                     /locus_tag="ACG8XC_000705"
     CDS             complement(23120..24979)
                     /locus_tag="ACG8XC_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9190425.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24980..25120
                     /locus_tag="ACG8XC_000706"
     CDS             24980..25120
                     /locus_tag="ACG8XC_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFP9190426.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(25097..26257)
                     /locus_tag="ACG8XC_000707"
     CDS             complement(25097..26257)
                     /locus_tag="ACG8XC_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9190427.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26564..27988)
                     /gene="araA"
                     /locus_tag="ACG8XC_000708"
     CDS             complement(26564..27988)
                     /gene="araA"
                     /locus_tag="ACG8XC_000708"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP9190428.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(28004..28702)
                     /locus_tag="ACG8XC_000709"
     CDS             complement(28004..28702)
                     /locus_tag="ACG8XC_000709"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9190429.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28659..30320)
                     /locus_tag="ACG8XC_000710"
     CDS             complement(28659..30320)
                     /locus_tag="ACG8XC_000710"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP9190430.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30538..31938
                     /locus_tag="ACG8XC_000711"
     CDS             30538..31938
                     /locus_tag="ACG8XC_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFP9190431.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(32190..33269)
                     /locus_tag="ACG8XC_000712"
     CDS             complement(32190..33269)
                     /locus_tag="ACG8XC_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9190432.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIIEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33355..34314)
                     /locus_tag="ACG8XC_000713"
     CDS             complement(33355..34314)
                     /locus_tag="ACG8XC_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFP9190433.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34326..35836)
                     /locus_tag="ACG8XC_000714"
                     /pseudo
     CDS             complement(34326..35836)
                     /locus_tag="ACG8XC_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
BASE COUNT        10187 a         7542 c         6348 g        11909 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagcgaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctctctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tcctgccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggtttccg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgatttttc gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgacgttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacatgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc agacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag acgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa
    13381 ttttgtgaaa atggctttct acatttttgc ataaatttgg cttaataccg agaaactggc
    13441 ttttgaacac cgtttaccaa gaggttgtga gcctgccgtt taactccgag tcacaaggag
    13501 caatttcaaa aaacagcttc tcaggtttgt tgactattgt gacttgtgac cgaggagttg
    13561 gcaggagaac accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga
    13621 gatacaaaac ggaaaaatag cttctcatat tttgccgttt tttgagctaa taccgaagga
    13681 cagctgattg aacaccgttt attcggaaaa agggactgtg acacgacttt tgtcacagcc
    13741 cctaaaaaaa ataataaatt tgtactattc tatgaagctt cttcttcaga aaactgactg
    13801 ttataaagat ctgcatagaa tccgtctttt tccatcaatg tatcgtggtt tcctgtttct
    13861 acgatcgaac catgattcat cacaataatg ttgtctgcat cgcgtattgt agacaagcgg
    13921 tgagcaacaa cgaaacttgt ccggttttcc aataggcggt tcatcgcttt ttgaatcagt
    13981 atttctgttc gtgtatctac actagatgtt gcttcatcaa ggatcaggac atctggattt
    14041 gctaagaatg cccgcgcaat tgtgattagc tgacgctgcc cttgcgaaat attgcttgca
    14101 tcctcgttta atacagtgtt atatccttct ggcaatttac gaacaaaatc gtctacgtga
    14161 gcagctttag cagcctcaat gatttgttcg tctgttgcat cgtcgttccc atatttgata
    14221 ttgtcataga tacttcctgt aaacagccaa gtatcttgca gtaccattga aaactgtgcg
    14281 cgcaattcat cacgtgaaag atcacgggta tctacaccat cgtaacgaat actacctcca
    14341 gaaacatcgt agaaccgttc aagaaggttg atcagtgtcg tcttccctgc accagtcgga
    14401 cctacaatag cgaccatctc accaggtttg acatccaagt taaaatcagt cattagcaag
    14461 ttatctggtg tgtagccgaa ttgaacattt tcaaaacgaa ctttgtatgg tgtatctttt
    14521 tcttctggaa ggccagattt ttcattggac atctcttctt catccaacac ttcaaatacc
    14581 cgttcggctg aagcaactgt tgattggata gtgttcatca aacttgcaag ttgagtgatt
    14641 ggttgagaga actgtgttgt atattgcaag aacgcttgaa catcccctaa tgtcatgttc
    14701 ccatttgcaa cttttactcc gcctagtaca gcgacgaaca catagcctag atttttaacg
    14761 aaattcatca acggcatgat gattgccgaa ataaattgag ctttccaacc agcggcatac
    14821 agattctgat tttctttttc aaatacttcc tggtcttttt cagtatgatt gaatgtttta
    14881 acaatggtat gaccaccata tgtttcttct acttggttat tcaataaacc taaacttttt
    14941 tgctgatctg cgaaaaacac ttgtgatttc ggcgctacga tcatcacgat gatcaagctt
    15001 aaagggatca tcaataacgc gactaacgtc aattgccagc tgattgaaag catcatcacg
    15061 atgaccccaa tcaatgtgac tgcactagtt acaaattgag tcaaattttg ctgcagcgtg
    15121 cttgcgatgt tatccatatc attgattgct cgagacatga tatccccatt tgtatgtgta
    15181 tcataatagg aaatcggtac acgattcatt ttggcttcca aatcatgacg taattcataa
    15241 actgttcgtt gggaaacacg tgtcataata aattgctgta aaaagttgaa tacagcagaa
    15301 atcaaataca tcacgattac tgtcgcaatg atctgcgcga ttttatcaaa gtctatcgga
    15361 aattttgtta cttgttggcc ttgttgcatc tgttgagcgc cagccattac acctttgaat
    15421 atttctgtgg ttgctttccc caagaccttt ggtgtttgga tttggaagat cacggctgct
    15481 attgctagta caaatacagc gatgatcgca accatacgtt tagacatata gccaaacaat
    15541 cggcgtaccg ttccccagaa atttttaggc ttttcacctt ttacaccgag atttctgcca
    15601 ggtccaccat gtcctggacc gcctgctggt ccatgagagg gacgtgtgtt tgttttactc
    15661 atgctaagtc ctcctctctt agttgtgaat gaacgatttc ttgataagtt tcgtttgttg
    15721 ctaatagttc ttcatgtgtt cctttgccaa ccagttttcc ttcatctagc acaaggatca
    15781 tatctgcatc cattactgta cttactcgtt gagcaactaa aacggtgata ctttctttca
    15841 tattcgtttt caatgcttgg cgaagattgg catccgtttt gaaatccaac gctgagaagg
    15901 aatcatcaaa cacatagata tctgctgttt tgactaatgc ccgtgcaatt gccagacgct
    15961 gtctttgtcc accagagaag tttcctcctc cttgttccac atggctatct aagccatctt
    16021 ccatctctga aacaaaatct ttagcttgtg cgatttctaa tgctttccaa atcatttcat
    16081 ctgttgcatc cggcttacca tactgcatat tgtctcggat cgttccagag aacagaactg
    16141 ctttttgagg aacaaagccc attaattctc gcaaattata ttgagtcatg tcactaatgt
    16201 ccgttccatt tatttggatc gaaccagatt cgatgtcata taatcgaggg atcaaattga
    16261 ctaaggtcgt tttacctgaa ccagtccccc ctatgattgc gacgatctcg cccgacttgg
    16321 cttggaaatc aatatcttcc aatgcaagtt tttctgctcc atgatatcga taattgacat
    16381 gattgaaggc caatgttgcg ttttttccag caaaagaaac tgttttagga ttttcagggt
    16441 catggatacc gattttttca tccaacactt cattaatacg gtcagctgaa gcttgtgcac
    16501 gaggcaccat gataaaaata gcggaaagca tcataaagct gatcaagatc tgcatcgcat
    16561 aagtcataaa tgcaatcaga ttaccgactt ccaaagtcat atctgctatg tagtgtccac
    16621 caaaccaagt gattgctacg tttgtaccgc tcatgatcaa tgtcatcatt ggcatcatca
    16681 atgcgacgat cgtattaact ttgattgccg tatttgtata atctttattc gctaaatcaa
    16741 aacgattttc ttcaaagcgt gtcttatcga atgctcgaat cactctgacc cctgtcaatc
    16801 cttctcgaaa taccaggttt aaacggtcag ttttcttttg catacttttg aataatggaa
    16861 cagcaaagta catgattccg ccgatcaaaa taatcattac tgggatgaca actaagaaga
    16921 tttttgtcaa ctgatgatct ttttgataag caagtatgct cgcgccaatc aacgtgattg
    16981 gtgcattgat catcatacgt aaaaacattt gtgtaaccat ttggatttga gtcacatcat
    17041 tggtcgttct tgtgatcaaa gaggccgttc catatttatc aaacgcatct tttgtcagac
    17101 tctctgattt tttatagaca tccgtacgca attgcttacc cagtttctgt gattcacgag
    17161 tagcaaaaaa tgtatttgcg atagcagcta agatactgat caaagaaaag ccgatcatca
    17221 caaaacctgt atgccaaata taatcgacat ctccttgcgc taccccttta tcaataatat
    17281 tggaggtaag tgtcggcaaa tataaatctg ccagtacttg gatcaccatg aaaactgcag
    17341 ctgcaattgc tgaagtcaag gagatccgtt ttacgatttt tagcatctag ttcccttctt
    17401 tctatttata tactccgttt ttcagccaag aaagtttcaa attgaattca ctgattgctt
    17461 gctctactga gtaatcttca gatgttgaaa ataagcgata cgaatgcgca attgttgtaa
    17521 atacagtatt catcactaat tggatcagta attccaattc atgatcattt tttactttca
    17581 gcttcgtccg atcgatcaat tcaagcaact cttttgcatc ttgtttgtgt tctttatgtg
    17641 gatcgtgagg cgttttttgg cgactcgtat gaggagcgac ttttcttgag acacggtaat
    17701 ccatgttcat aaacaagtta cgataaaaag aagcattctc gccttccaaa atctcataaa
    17761 tcattttaga aaaataggat tcgaatcctt tgaatagatc accatcggat tctttcatta
    17821 gttgtaacat atctttagag ctgtcacgtc tcattgtctc aaagtaataa aaataaagat
    17881 cctctttatc ttcaaaatac tgatagaaac tgcctctagg gatatccgct aatttgacga
    17941 tattagcaat cgaagcttct tttaagggta ctctagaaaa ctcgattctc gcagcctcga
    18001 ttagtctttt ttgtttttct ttaggcaaat ggaaaaatgt ctgtgtcggc atatggattc
    18061 tcctttcttt caagatgaca cattgtcata tgacgctatg tcattataac acctcctacc
    18121 tgttttgcaa gacatttgca tagttttttt cattatcatc cgatacatta aaaaagtgat
    18181 aagaggaatc cttccatttg aattaatgag agttgcccat taaaatagcc acatactaaa
    18241 caaaggatga gataaatggc ccaatcaatt agtgtccaag acttcaataa attgcctctc
    18301 gatgctgaca gcatcgtttt agatacaaga aataccttag attacacaaa aaaccactta
    18361 agccactcgc tttctatccc cgctgctatc ttgccaagac aattaaaaaa tctagatcca
    18421 aagaaacttt attatattct aagctataca ggcaggcgat cagaagcatt agccgatcaa
    18481 cttactgcta atggttttcg tgctgtttat atcattggtg ggatggaagc tcttcagtcg
    18541 agtgttgctt gaatattgct tgttagattc aaaagaggct gtgacaaaaa atcgtgtcac
    18601 agcctctttt ataccctgtc tttttttagt tatttattct tcaaccttat caatatgact
    18661 tgatagcctt ttctttttcg agtaaactga ttttgttagt catagttata acaatcatag
    18721 caataaaagg agaaggtaag catatggcat ctgttttagt agtcaaaggt catcccctaa
    18781 ctgcagaaga atcgcgcaca gttaaggcat tgacgtcttt tttaacaagt tataaagaaa
    18841 atcatccaga tgatgaagtg actgttttgg aactttatcg cgatgatatt ccggaaatcg
    18901 atgaagaatt attatcaggt tgggaagcat tacgtgctgg tgcagaattc acttccttat
    18961 cagaaagtca acaacaaaaa attgctcgtt tcaatgaatt gactgatcag ttccttgctg
    19021 ctgataaaat cgttatcgca aacgccttat ggaatctaaa tatcccaaca aaattaaaag
    19081 cttggttcga taccgtcaat gtagctggaa aaacgttccg ctatacagaa aacgggccag
    19141 aaggattagt gacaggtaaa aaagcactgc acatccaatc taatggcggt gtatacaacg
    19201 gacaagactt tgcctctcaa tatgtaaaag gtattttaaa ctttgtaggc atcgatcaag
    19261 tagatcagct attcatcgaa ggaatcgatt atgaccctga ccgtgcagat gagttgatgc
    19321 aaaatgcatt agataaagct gctgctttag gtaaatcatt ttaaaaattg agataaatgc
    19381 atacaaaaga gacagaggaa aaaatcttca aagcagcatt tgtgccttgg atattttccc
    19441 atctgtctct ttttgttatt ctttctgctt tttcaacgga ttttcttcat aaagagaaga
    19501 gattgttgtg taccattcaa aaacatgagt aatcactact ttagctgctg cataaacagg
    19561 aatccccaag atcacaccga tgattccgaa caatttccca gaagtcagca gaactaataa
    19621 aatcgttact ggatggattg ctaactgatt ccctagtact aatggagaga tcaagcgtcc
    19681 ttctattgtc tgctctactg caaaaacgat cagtactttg atgatcatga atggacctgc
    19741 tacaatggct aaaaagatag ccggaatcat cgctaaaaat gatcctaagt aagggatcag
    19801 gtttaaaaaa cccgcaataa tccccaacgt gacagcatac tctaaaccaa tgaccgcaaa
    19861 accaatcata aacatgattg ccacagcaaa agccacagtt agttgtcctc ttatataaga
    19921 agaaacttga ctattcattt cactcagcac attcaacgtc ggtttttgca tgcgtgtcgg
    19981 taaaaatttg acaaaataag gtgctaattg tttaccgtct ttcaatagat aaaataagat
    20041 aaacggcatc gtcagcagag ccactaaaat agtcgctacc gcgccgacaa aacttccaag
    20101 actctgaact gtactcttag atatatcttg gatcatatcg cctgccgaat tcatgaactt
    20161 atcacctgct gtttccaact gttcgcgaaa ttggttgaac agcgggtccc gcaaaatctc
    20221 ggttaattta ttatcaatcg tatctacata ttgagggaag ttactgatga agctcactgt
    20281 ttgttcctga atcttaggga tgatcactac gctcccccat gcgattagtg caacaaccaa
    20341 aacaaacagc ccaatgatac tgtaaagcct tggtactttt cgcttttcaa agtaatcaac
    20401 taccggattc atcaagtaat acaagattcc tgctaaaatg ataggcagac ctacgattgc
    20461 taaaaattgc cagattggtg caaacaaata tgaaactttt gtaaataaaa agacgatcag
    20521 caatattaac aatacaacaa gtaaggctgt gactacttga ttatttaaaa accagcgcca
    20581 aaaccacgat accggtctgt tcttcttatt ttttccttcc ataattcctc ctgttagtta
    20641 taaaaaattt ggcttcccaa accaatagca ggcagtctat gcggatgaag atagattcca
    20701 tttgcaattt gtccttcttc tggacaagca tcaaaaatca atgatacgtg agctactgtc
    20761 tctaaattcc cattggccat cggaccgaca taatcgatcg tatatacctg atcatcaaaa
    20821 gcaattgttc cgcctgcttt caaagaaaaa ggttcgtctt ttgatacttc ttgaatcacc
    20881 gaatattttc gtaaggcagc tgttgctgtt ttatcgaaca aaatcagcat gggttcttcc
    20941 ttgtttattg cttctgtccc aatttctgta atctttgcct gaatcattgc actctctcct
    21001 ttaattgctt tcaaaaacag tataacacat tctcattctt tagccgtgtg gaataattat
    21061 gtgatagaat aaaaacgaaa actatttcat tacaattagg aggaatcagc tcatatgatc
    21121 gaaaagataa ttttaggaac ctatacgcgt cgcgaaagta aaggaatcta tacgatcgac
    21181 cttgatacag aaaaagaaga attagccaac ctttcattag ctgttgaaga aaatagccct
    21241 acctatgtag caaaaagtaa agctggcaat ctttacacag ttactagtgt ggatggatta
    21301 ggtggtgccg gtgcgtacga taaggatttt cattttttga atgcggtaac agagtctggc
    21361 gctcctttgt gttatgtagc ggttgatgaa gcacgtcaat tactatacgg ggcgaactat
    21421 cataaaggtg aagtaaatgt ctaccatata ttaggagatg gcgaactgaa agcagcagat
    21481 tcaatattcc atcaagaagc gacaggtcct cataaaaacc aagatcatgc gcatgtccac
    21541 tatacggatc tgactcctga ccaacgatta gtcgtatgtg atcttggtac agatcgtgtg
    21601 tatacttatg atgtgtccga aaacggcaaa ttgaatcttg taactacctt cactgcagag
    21661 ccaggaacag gccctcgcca tcttgtcttt catccaaatg gacaattcgc ttatttattt
    21721 ggtgaattag acagcacagt ccgcgtgctt tcttataata aagaaacagg tgcttttgaa
    21781 gaattacaga aaatatcgac cttgccagaa gaattcgacg gtgaaaatgg aggggcagct
    21841 atccgcattt caaatgatgg caagttcctt tatgcttcta accgaggaca taactcgatt
    21901 gctgtattcg cagtttttga agatggttcc cacattcaaa acaagcaaat catctcaaca
    21961 gaaggggatt ttccccgcga tttcgcacta gatccaagca ataattttgt cgtgtgtgcc
    22021 aatcagaaca ccgataatct gacattatat cgccgtcatc ctgaaacagg attactggaa
    22081 ttactgaaaa aagatgtcta tgtaccagaa tgtgtctgtg tttattttga tcgttagtaa
    22141 gaaaaggaga atgaaatgaa tcagcaagac gtattagaca gactacggga agaactaaat
    22201 attcctttct ttgatgggaa aatcgaagat aaagagtata gcgaagagga atatcagaaa
    22261 ctcaaagccg atttacagaa ctactttgaa caatatgtac agaatgtaga aaactaatca
    22321 gctttccata ctatctgaaa agaacatcag gacaagactt ttccgtctat cctgatgttc
    22381 ttttctacaa ataaaaaacc gccgagatgg ttagactcga cggaaaagga gtatttactc
    22441 gattttgagt aagtacaagg ttgttgttgg ttataggtat gtcattatca taaaggattt
    22501 tgatcactct atcataaagg taatattaca tttatattag ataactctag tttttattgt
    22561 tatcaaagat ttcttttatt ttttcaacaa caaattagtc attttttgga tggcagctgt
    22621 caattggtct gtcgcttggt cttgttttgc ttcagatgta ttgaatagat cttcatctac
    22681 ccctgattca agatcgatac tttggatcat ttcttcacag cctgctacat atgaaagata
    22741 agctttttca aaatttttat gaatgcccat tgcttttgct ggtggacgca aagtactgac
    22801 tttcataagc attgcccgat atttttttgt tccttcttca aataacagga tgatctcttc
    22861 aagagtcgat ttgtccagtt cacttgtttt tccttcatca attgcgtttc tgatcacttc
    22921 atatttcgga tgcattcctt ctccgattgc ttctgtttcc ttgacgattt gatcgatgga
    22981 ctgcacataa tagccaatat tcggtaacat gttcgttttc ctactttctc cattgttgtt
    23041 attatttatt ctacttgttt ttatcctcaa atgacaagta cttgaaaaaa tttaatggtt
    23101 tctatacagc tcgccattct tattcgtgct caaagaaacc atctgctaaa ctatattgtt
    23161 tccctgcgac aatgtcatat tggatcaatt gatattcgtc gtaaatctct tgttgcgagc
    23221 gattgaattg cgcttcttta taccactgtc cgttttgata gtacagttct cgttcaaacg
    23281 ccggaagttc ctgttctaat gataaaagca gctgataaaa gccatttgtt gtctgattcg
    23341 tctgttccat tagattcgct gcaaaataaa acggactggt gattgcatcg tgagtcgttt
    23401 gcttttctaa ttctccttta ctgtcgaaca taagaaattg tgtttcatgc aatgtttgtt
    23461 tgtcattttt tgcttggatc gtatctgaat agatccctgg aagatgatcc ccccaaaaaa
    23521 caactaaagt ccttctagaa agattcttca attcttccgt gaactgcttc agtgccgtgc
    23581 tgctatagga aatatcttgc agataattct ctaagtcaag tgtcccactt ccatctgaag
    23641 tttttgcagg atagtctaga ttatcatact ttccatcgta gggcatatgt gtctgcatcg
    23701 tgactaaatg aatgaactga ggtgtttttt cttccttgag aagtgtcatt acttctttgt
    23761 aagctgactc atcagaaata tatggattat tctctatcgt atccgtatag gtcatcgtac
    23821 gctcgctgat gaactggtca aatcctaatg tctgataaac atcctctctt ttatacatcg
    23881 aagtattgta tggatggatc gctgtcgtct ggtatgattg tgcgtttaac gctgaaacga
    23941 tagaaggaag ctgatccatt tttggtacaa gcatcgtgta aggggtggtg agttgcgcat
    24001 taaataacgc catagaaaaa ccagtcaatg cttcaaattc gatatttgct gttccaccgc
    24061 catagttttg agaaagcata ttgccactat atgtttgatc agctacttca taataatctg
    24121 ccaatggctc accagatact tctacgccgt ttaatcgact aggatctgag aaactttcac
    24181 tcatcacgaa cacgatgttc ggcgactctt cctctacagt tttatttttt tcatcggcta
    24241 gcttctggta tttttcagtg atttctttga tcttttcttt cgaatatcct tcaggttcat
    24301 ccattggttc taccttcaga ttgtacaaaa acccaccgat aaaaccagtg ttatagtagt
    24361 tcattttctg gctgtaaggt atccatagtg ccgttttatt atatgctttt cgcaacaaat
    24421 tatcgggatt gttgaaatta ctgatatacc ctagcagtcc aattgccaca agcagtgtcg
    24481 ttaatcggat caattggaat cgccttcctt ttttcaggct tcgaaacatg taccagcaaa
    24541 acaatcctat cacggttcct gcggcagcta agatcactgt aaacagcata gtgccggtca
    24601 tatctttcag caatccgatt tcagtgatca tcttcaaatc gtctggataa atcggttctt
    24661 gccgataact cattttcata taatttgcaa aacctaatac accgatggct acgacatacg
    24721 taagggaacc taaagggaca gatcctgcta gtgcaatcag aaaaataaga ataatcagca
    24781 agacgagaca ggctaagaag aatttttccg tatgccaaga aaaagcaaat ttcaacgcaa
    24841 ggtctacgga tagatcattt tgacaccact gcaaaaataa attactaaaa acaacaagaa
    24901 aaaagatact gatgattgct gctgttcctt ttaaaatctt tttctgtttg gtcgaaagtc
    24961 tgcttatata tcttttcaaa tgcttgctcc ttcatttcta tcaataaaag aatgttgctt
    25021 ctcttatttt agttgtacga aattcgaaag taaagaacgg aaagaagaat taatgaaatc
    25081 caaatattct ctttctttat ggcctttctg gtttctttaa taagcggacc tttcgtactc
    25141 cagaaatttt ttctagctgt tgtttcactt gattgagctg ttcaaaatca gtactgtcta
    25201 tgtcaatcaa tatgtagaca tgctcattct tatggttacg cgatatatct gcgatcgcta
    25261 gctcgttctc attcagcaga gaaaatatct ctgctaatat attggttttc ttttgataaa
    25321 atatagtgaa tcgaaacggc gcttggaaaa acattcgtgc ggatggataa ttgaccgctt
    25381 ctctaaccgt accgaacaaa agataatctt ttaaggcttc aactgccaaa cgtccgctgt
    25441 ctgccaaagc ttcctgagtc gatcccccta gatgaggcaa cataagtata cggtcatttt
    25501 ctaagaactc ttcttctgga aaatcagtca agtatttagc cagatgttgt ttttccaatg
    25561 cttgcataac agcatattta tcaacaattt cacttcttcc tacattgata agtacagcat
    25621 cttttttcat tttttctata ttcgcctgat cgatcaatcc ttttgtatct tctgtcaacg
    25681 gcagcatgac tacgatgtaa tccgaggtag aaagaactgt ttctaaatct gcttgccgaa
    25741 cataatccaa ctgggcatcg cggatagaat aacccaagac atccatccct aagctatagc
    25801 aacttagcgc tacttttgtg cctatcgctc caagaccaag aagaccgatg gttttgcctt
    25861 gcaattctcg tcccacataa gcacttcttt tgttttctgc ctgttctaaa atattaggac
    25921 ctgttagtgt ttgtaccata cgacttgctt cgattatcgg acgggaagat aagagcaaac
    25981 agcacaaaac aagctctttt actgcattag catttacacc tggtgtattc atgacgatcg
    26041 tcccgttttc agaagctttt tcaacgttga tcgtgttcac cccaacacca gctcttgaaa
    26101 ttgctagcaa atctggtgta agccattcat ctttcacttt cgtactacga ataactattc
    26161 cttcggctgg caaatcatct attattttga aatattcccc ttgtaacttt tctatctctt
    26221 gttctggaat agcatcaatc aaatgaacgt gatacataaa accccctcct tactaacaag
    26281 tatctcgaaa tttctgcaat tagacaactg tttaccattt gacttcctag aagcaaaaag
    26341 agcagttaga gcagagtagg ctgagtgata aaaataaggc tatgacaagt tactgccata
    26401 agccttattt tcgagagcaa aatattgagg gtgagatatc ttgataaatg gtgttccgtt
    26461 agctgacatt gataataaga ctaaatttca aaaaagttga aaagcaaatt tcgtcaatct
    26521 cttcttattt cttaaaacta agtggctttt tcacaacctc tttttatcta ataaccactg
    26581 tttccaactc aactaatttt gcccacgttt caatttgatc tgttgttaga tttagcgaga
    26641 caacggtatg atgaccgcca cctgcctgaa tccatgaatg gacaccttca tggaaattcg
    26701 gtttagtttt ccataacaca cgtgcaacag gtaaattagg tgcttcagtc gttggttcaa
    26761 aggcttctac ttcattgatc agcagtttat aatgcgtacc gaaatctgcc atcgatacga
    26821 ctaccccttc tcctgctttc ccatcaaata caagacgcgc cggatcttct ctatctccca
    26881 ttgataaagg agaaacaacg attcttggtt tcgtcgcagc aagcgtcgga tctacttcca
    26941 tcatatggga ttccaagatt gcttcttggc cactagctag ctcgtaagta taatcttcca
    27001 tgaatcctgt atctttccct cgagccatga ttttcagcaa ccgatcaatc gctgcagttt
    27061 tccagtcgcc ttccccagca aaaccgtatc cttctgccat caaacgttgt acagctagtc
    27121 caggtagttg tttcattcca tacaggtctt caaaattagt cgtaaaagcg ttatagcctc
    27181 cttcttctaa gaaacggcga ataccgattt cctgctgagc ttgtactttt acatgttcct
    27241 cccatgtctt ttcttcataa tcaccatagt cgaaggtata caattccttg tactcttcaa
    27301 ataattgatc gacttcttcg tctgatacgc gatcgattac ttgtacaaga tctccgatac
    27361 caaaataatc aacagtccaa ccaaattgaa tctgtgcttc cactttgtct ccttctgtga
    27421 cacccacgtt tctcatgttg tcaccaaaac gagccacttt tattccaaaa ctttcattat
    27481 aggctactgc aacatccatc caatcagcaa tctctttttg gatctcaggt cgtttccagt
    27541 agccaacgac gattttattt tgtttattta atcgagcatt gatgaaccca tattcgcggt
    27601 caccatgagc ggattgattt aaattcatga aatccatatc gatcgttttc caaggaatgc
    27661 tttcgttata ttgcgtagct aaatgaagca atggtttttg tagaagcttt gtcccgcgga
    27721 tccacatttt tgctggtgag aaagtatgca tccaagtgat cacacctgct acttcttctc
    27781 gataattgac ctctttcatg atttttgtga tcgtatcagc agtaactgct aattcttgaa
    27841 ggcggattgg ataaggcaat tggccatttt cattcagttc atcgaccatt tcttgtgcat
    27901 gtttctttac ttctcgcagt gcttcttccc catataaatg ctgtgatcct acaacaaacc
    27961 aaaattcttt ttctccaata ttcaacataa ttagccactc cttttatttg ttttgtccgt
    28021 aatacgcatt ttttccgtgt tttcgtaaat aatgtttatc taaaatccgt tgaggcagct
    28081 cttctgaaaa tgaattgagc tgtctggtaa ataaattcat cttgcatact tcatctagta
    28141 cgactgcgtt cattaccgca ctttgcgcat ctttccccca agtaaatgga ccgtgtccgt
    28201 gtaaaagtac ccctggaatt gctaagggat caattttgcg ttctttgaat gtttctatga
    28261 taacttttcc tgtttcatat tcgtacccgc tgtcgatttc ctgttgtgtc aaaaatcgag
    28321 cacaaggtac cgatccataa aaagtatctg catgcgtcgt ccccatcgca ggaacatcca
    28381 atcctgcttg tgcccagatc gtcgcccatg tcgagtgagt atggacgatt cctccgattt
    28441 gaggaaatga acgatataaa acagcatgag taggcgtatc cgaagaggga ttgagtttac
    28501 cttcaatgat atgattgtcc aagtccacaa cgaccatgtc gtctgctgtc atctcttcat
    28561 aactgactcc gctaggtttg ataacgaaat atcgtgtttc aggatcaaaa gcgctcacat
    28621 ttccccatgt atatttgacg agtccgtgtt taggcaagtc aagatttgct tggtatactt
    28681 cttctttcag ttgttccagc attttctatc actccttttt cttgcaattg atgaatcgct
    28741 gttttttcaa tgtcgatccc ctcacggtaa cgtctcataa atatctgata gcctgctatt
    28801 tcttctgctg aaggtgtgat ggtgattcct tggtcttgtc caaacacgtc ttctgccaag
    28861 aagttttcta aagacttccc atgtcgatcc atcatatagg ctgctaataa cgcgattccc
    28921 cacgctccac cttctccggc tgtgtccatc actgtaactg gtgcctccat agcagatgct
    28981 agaatccgct gtccaacttc tggtgtttta aaaatccccc catgccccac taaacgatca
    29041 atttggacat tctcagattt caatatatcc attccgattc tcattgcacc aaaagcactg
    29101 gctaaatgga tccgcatgaa attcgctaaa tcaaaacggc tacctggtgt acgtacaaat
    29161 aatggccttc cttcatttac tcctgtaatg ttttcaccgg aaaaataacc ataagaaagt
    29221 aatcccccgc aatccgtatc gccttctagc gctttgttga ataataccgt gaataattta
    29281 tctctgctta tctccatacc caaagaattg gtaaattctt caaaaatact gacccaagca
    29341 ttgatatccg aagagcagtt attcgtatga accatgccta ctagttctcc tgaaggtgta
    29401 gtgaccatgt cgatttccgg atggacattt tctagttctt tttctaatac gatcatagcg
    29461 aaggcggaag taccagccga aatgttccct gtccctttgg ctacactgtt cgtagcgacc
    29521 ataccggttc ctgcatcccc ttctggcgga caaaccggga ttcctggtct caaatttccc
    29581 gtagggtcga tcagataagc ccctgattcg cttaacttgc cagcttcttc tcctgctact
    29641 agaactttag gcaaaatgtg atccaagtca atcgctacgt gcgctttttc gattaactga
    29701 ccaaaaatat ttttcatttc ttgatggtag gttttcgttg aaggatcgat aggaaacatg
    29761 cctgatgcat ctcctacgcc tagtactttc tcaccagtaa gctgccaatg gacataacct
    29821 gcaagcgttg tcaaaaaatt aatttctttc acatgttctt ctttattcaa aatagattga
    29881 tacaaatgag cgatactcca tctctgcgga atattgtatt gaaatttttc cgttaatatt
    29941 cgttctgctt cttcagtgat tgtatttcgc caagtacgaa atggtactag taactgttcc
    30001 ttgctatcaa atgctagata accgtgcatc attgcactga acccaatcga cccgattgtc
    30061 gtaagattct cgccatagat ttcgaatact tctgctgcca tttttcggta actgacgcgc
    30121 agtcctttcc aaatatcttc taatgaatag gtccagatac cattttctag tttgttttcc
    30181 cactcataac taccagcggc aataggagta aaagatgaat caatcaatac agctttgatc
    30241 ctagttgatc caaactcaat ccctaaagat gtacgaccat ctatgacatc taacatactt
    30301 tgcgcattta cttccttcat aatgatttaa ctccctcctt ctcgtcaaac ctgatcaaac
    30361 cgtcttttta gtagcgctta caatgattat tgtatacttt tgtacgtaca tgtcaagcag
    30421 ttatttacaa gaatacaata aaaaacactt gttttgtacg tacataagtt ataaaatgaa
    30481 attgcttaca aaatctgatc aaccgccttc tcgaaattct agaaggagag aatacttatg
    30541 aaaaaagaaa aaaaagtttc ctcatcgttt atttattttt ttggcgcatt tggcggcatt
    30601 ctatttggat atgatattgg tgttatgacc ggcgccttac cttttttaca gcatgattgg
    30661 ggtttggcag gtaaagcttc tttgatagga tggatcacgt cttcggtcat gttaggtgcg
    30721 atccttggcg gctctttatc tggtcttctt tctgataagc ttggacgacg aaaaatgatt
    30781 cttctttctg cacttatttt tatggctggt tctgtccttt ctgcatctgc ccctcacaat
    30841 ggttcttatt ttctgattgc agcaaggatt cttctaggtt tagctgttgg cgctgcatct
    30901 gccctagttc ctgcttatat gtctgaaatg gctcctgcta gactaagggg aagattgtca
    30961 gggatcaatc aagtgatgat tgcttctggc atgctccttt cctatgttgc agattatttg
    31021 ctaaaagggt tgcctgaaac gatggcttgg cgcgtgatgt tagggctcgc tgctgtcccc
    31081 gcgctgattt tatttttcgg tgttttagct ctaccagagt caccgcgttt tcttatgcaa
    31141 tctggccgct tggaagaggc aaaaagagta ttgaactata ttcggacacc aaaagaagct
    31201 gaacaggaat tcgaacaaat ccagctgaat gtcaaacaag aaaagacaac cggaacttct
    31261 tggcatactt tgtttttaga aaaatatcgt tccttagtca ttgccggtat tggtgtagca
    31321 gtattccagc aatttcaagg agctaatgct attttttact atattccgct gatcgtagaa
    31381 aaagcgactg ggcatgcagc gagcgatgca ttgatgtggc cgatcatcca aggaatcatt
    31441 ttagttgctg gttctttgct ctttttggtt attgcagaca agttcaatcg gcgaacttta
    31501 ttgaaaatag gcggtagcgt gatgggactt tctttcatcc taccagctgt tttaggaact
    31561 gtactggatg ctcatacaaa ttcgttgttg attctcttgt ttttatgtat ttatgtcgca
    31621 ttttattctt gcacatgggc tccactgaca tgggtaatcg taggcgaaat attcccacta
    31681 gctgtccgcg ggcgagcatc tggcctagcc tcttctttta attggattgg ttctttccta
    31741 gttggcttgc tttttcctgt gatgactgct tctatgtctc aagaaatcgt tttcggcatt
    31801 tttgggatca tctgcttttt aggggtttta tttattcaag agatcgtacc agaaactcgt
    31861 gggaaatctt tagaggaaat cgaacaaagt gcatctaaaa aaacttatcc taaacgtatt
    31921 tctatccatc attcttaaag aggttgtgac agaagtggtc agcttcaaga aataagaagg
    31981 aaccgcttag tttccagtca caaggaacaa tttcaaaaaa cagcttctca tgtttgatga
    32041 atattgtgac ttgtgaccag ttggtaggag aacaccgttt agtggagagg tggtgaaaga
    32101 aacgttcagc cttgagcgat gacaaaagct ctcacaaaca tcgcgacgag taaccgtagg
    32161 agcaataaga ggcggtaaaa acccgtctct tatttttcgt tttctttcac tgtttcccgt
    32221 tcaactagta caggttcata gagaatattt ggctgtgcct gtcctttttc aatcgttgtg
    32281 acgatccacc gtgcggcatc ttgtcccaac ttttcttttg gatgcgtcaa tgttgtcaat
    32341 tttttattcc ctaaatggct taaagaagaa tcgtctgttc ctacgatcga taggtcatct
    32401 ggaatatgat agccattttc tgtcaactta tctagcatta aactagctat ttgatcgtta
    32461 tagcaaatga tgccatctaa agcttgttct tctttcaacc gttgtactgt tttttcagcg
    32521 attgtttctc gtgtttccgt ggtgaaagta atgatatctt ccgtcaaaaa acgcacatta
    32581 tactgttcac acgcgctgat aaaacctttc atcctgtatt ttccttgaag atcgtcgatt
    32641 tttgtgatga acaacagacg ctgatgtcct ttttttatca aatattctgc tgccataaaa
    32701 ccgactttca catcatctaa acaaatataa gaggcattta actcttcata agcagcattg
    32761 atcatcacca tcggaatgcc ttgttctctc aaagcaacat aaagtgctaa gtttgggtta
    32821 tactgattac ttttcgtagg ctcaatgatc aaaccatcta ccccataagc aatcattttc
    32881 ttcaaacact ctttctccaa ttcgtgatcg ttattcgtac tggctaaaag caatgaatac
    32941 ccagattctc gtaattcatt ttcgattcct cggataatcg acggaaaaat ataatcagag
    33001 agatatgtgg tgatgacacc gatcgttttt gttctgttca atggtgtttt ttctttctta
    33061 tagctgtcat ctacatatgt gcccgaccct ttttctttac gcagatagcc ctcatttacc
    33121 aacacagcga ttgcttgccg aatcgtatgt ctgctgacgt catatatttc ttgcagcttg
    33181 aattctgagg gaatggttgt atttacttcg tattcctcac gaaggatctt ttgttttatc
    33241 tcttctgcaa tatcttgata ttttgccatt tgccttgccc ctttttattt gtacgtacat
    33301 atatcataac gagtctgcaa ggtttattca agcaaaaatc aaggtttctt gtgctcataa
    33361 ggcatacgga acaggttcac caaattctgg aaatccttgc tcattccacg taaagcattg
    33421 cgcgttcgcg tgccgattag gattgtctaa cggatcccct ttttggttct tctctggtcg
    33481 agcatgatag atcaaaatat caaagcaccc gtcttctgaa accgtaaacg agttgtgacc
    33541 aggaccaaat tgcttatttt tttcagaaga aacgaatact ggctccgata atttatgcca
    33601 tgattctttt aataaaagat tcttgtgctc atcagcccac agcaggccca tggcatagtt
    33661 ttcatctgtt gcactaccag aatacgtaat gaatattttc ccattgcgag taaggactgc
    33721 tggaccttca tttactgaaa aaccccgttt ttcccattca tactcaggta tagaaaggag
    33781 gatctgcttc ccctttaacg tccaaggatt atccatttcg gatagataaa gattagaatt
    33841 cccagtaatt tcaggatctt gttgggccca cacataatat agctttcctt gagaagagaa
    33901 agttgttccg tctaaactga aagtttccct tttcgttttt atttgtccct tttctatcca
    33961 atctgtattc attggatcta tatgctgatt ttcaagtacg aacatgcggt gatgatgaga
    34021 ttcatcacgt atagttttat gattgcttgc agcaaagtaa atataccatt ttccattgat
    34081 ataatggact tcaggtgccc agatcaattc actcatcggt ccttcttcat gagcctgcca
    34141 tacaatcagg cttgcagcat gttctaattc attcagtgac tttgcccttc ttaattcaat
    34201 cacttggtag cctggaaccg atcctgtaaa ataataataa ccatctgtat gtttatatac
    34261 ccaaggatct gcccgttgcc gaacgatagg attattataa agtgtcttca tcataacaaa
    34321 tttcctcaca ttttctttat tctaattaca ttccaagagt atttttctat ccctacattt
    34381 aattcgtcat gtttttctat tgaaaaatag tttatttcag gttttatttt ttctggttct
    34441 tcacgtgtat ttttgtccaa cagatcgcta ctggttaatg tgtagtgctc tccttgatct
    34501 agcaaattga aatcatgttc aatcgttatc ttttgttgtt catttgtgcg attgatcaag
    34561 aaaatagtca gctgattgcc gttatctacg atcacactgt ccacataagg aacatttttt
    34621 atcttttcac aatcataaac agggcaaatg atttgttgtt tcaatacttg acctttagca
    34681 tatttcgata agtgttgcat cacataataa gttggattcc tccatgcttg tcctttagca
    34741 tctgtcagta ttaaaggtat tgtgtttact aacaatgatt gacaactaat ctttacgcga
    34801 tctgcgtgtc gtaaaatagc cagccctaat gaaccaaata ataatgtatc ttccatagta
    34861 aagtaacacc aatcgatggg actaccgact tcaaaatccc tatgtctttt ttccggttgg
    34921 gcatgtacat tccattcatc aaacgctaga tacattgttt tctcacttct caaatatcct
    34981 tttacataat cacaagtagc aacgatttct tcaatttgac gatcgaatcg ataagctttt
    35041 gcaaggtatg tcgctgtatc atcacgctca tttttaggag gtttagtcaa gtcttcttca
    35101 tcatacttat caatatagtt atgcaaagct aaataatcta cattctcata agcttcttct
    35161 aatgcgatac gatcccattc agggtacgat ggtaaacgtg gcgtagagct tccaactaag
    35221 attgtttcaa ttgaatcatc cactaacttc atgacttttg aggcttcatt tgctaaccta
    35281 ccatattcat aagcactctt agcccccact tgccatggac ctctagctca ttccctaaac
    35341 accataattt aacgccaaaa ggcttttttt gcccattttt tattcttaaa tcactccaga
    35401 aggttccttt tggaaaatta caatattcta ctaaattttt tgcttcatca attccttttg
    35461 ttcctaaatt caccgtgaat atcggtttcg cttgtacatg actgatccat ttaaaaaatt
    35521 cgtggagtcc aaaagaattt ggttctatac ctcgccatgc taaatcaatt ttttttggtc
    35581 gttgttcttt cctacctaca gtatccaccc aatcataacc agatgtaaag tttcctccag
    35641 gatagcgaat gacacctaat tctaattcct ttacataaga gaggacatct tgtctaaacc
    35701 cgtcaccatc agctgtttca tggcccggtt cataaatccc tgtatagacg acacttccca
    35761 tatgctcaac aaatgagcca aacagccgag aactgatctc tcctaccgta aaatcctctc
    35821 tgatttttac tttcacttct tcgtccaaca gctttcccta cctttcattt cctatagtca
    35881 agataataga tacaagtttc catacgccac cagcaaagat aaaaataaaa ggagggaaaa
    35941 gaaatatgag cagtacaaat cctaataaca atacgcaaca aagaat
//
LOCUS       DAZKCY010000016        35064 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000016 DAZKCY010000000
VERSION     DAZKCY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.016"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACG8XC_000715"
     CDS             complement(59..187)
                     /locus_tag="ACG8XC_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9190434.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8XC_000716"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8XC_000716"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9190435.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACG8XC_000717"
     CDS             complement(1674..2729)
                     /locus_tag="ACG8XC_000717"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9190436.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8XC_000718"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8XC_000718"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFP9190437.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8XC_000719"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8XC_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFP9190438.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8XC_000720"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8XC_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFP9190439.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8XC_000721"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8XC_000721"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP9190440.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8XC_000722"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8XC_000722"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFP9190441.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACG8XC_000723"
     CDS             10491..12050
                     /locus_tag="ACG8XC_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFP9190442.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACG8XC_000724"
     CDS             complement(12161..14092)
                     /locus_tag="ACG8XC_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFP9190443.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8XC_000725"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8XC_000725"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9190444.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACG8XC_000726"
     CDS             complement(15021..15773)
                     /locus_tag="ACG8XC_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9190445.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAIKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACG8XC_000727"
     CDS             complement(15932..16141)
                     /locus_tag="ACG8XC_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190446.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACG8XC_000728"
     CDS             complement(16152..17480)
                     /locus_tag="ACG8XC_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190447.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACG8XC_000729"
     CDS             17616..17771
                     /locus_tag="ACG8XC_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFP9190448.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8XC_000730"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8XC_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP9190449.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8XC_000731"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8XC_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFP9190450.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8XC_000732"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8XC_000732"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9190451.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACG8XC_000733"
     CDS             complement(21455..22387)
                     /locus_tag="ACG8XC_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190452.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACG8XC_000734"
     CDS             complement(22544..23203)
                     /locus_tag="ACG8XC_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFP9190453.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8XC_000735"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8XC_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9190454.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACG8XC_000736"
     CDS             complement(24244..24534)
                     /locus_tag="ACG8XC_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190455.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="ACG8XC_000737"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="ACG8XC_000737"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP9190456.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG8XC_000738"
     CDS             complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG8XC_000738"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP9190457.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="ACG8XC_000739"
     CDS             complement(28200..29369)
                     /locus_tag="ACG8XC_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFP9190458.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG8XC_000740"
     CDS             complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG8XC_000740"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFP9190459.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACG8XC_000741"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACG8XC_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP9190460.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="ACG8XC_000742"
     CDS             34320..34841
                     /locus_tag="ACG8XC_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFP9190461.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         9471 a         7619 c         6292 g        11682 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttta tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagcc cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attc
//
LOCUS       DAZKCY010000017        34397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000017 DAZKCY010000000
VERSION     DAZKCY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.017"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG8XC_000743"
     CDS             352..1041
                     /locus_tag="ACG8XC_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9190462.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG8XC_000744"
     CDS             1028..2236
                     /locus_tag="ACG8XC_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9190463.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG8XC_000745"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG8XC_000745"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP9190464.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8XC_000746"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8XC_000746"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP9190465.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACG8XC_000747"
     CDS             complement(6339..7034)
                     /locus_tag="ACG8XC_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFP9190466.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8XC_000748"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8XC_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP9190467.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACG8XC_000749"
     CDS             complement(8233..8424)
                     /locus_tag="ACG8XC_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFP9190468.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACG8XC_000750"
     CDS             complement(8445..9335)
                     /locus_tag="ACG8XC_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP9190469.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACG8XC_000751"
     CDS             complement(9322..10089)
                     /locus_tag="ACG8XC_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9190470.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8XC_000752"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8XC_000752"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP9190471.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8XC_000753"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8XC_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP9190472.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8XC_000754"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8XC_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP9190473.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACG8XC_000755"
     CDS             14660..15043
                     /locus_tag="ACG8XC_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190474.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8XC_000756"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8XC_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFP9190475.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACG8XC_000757"
     CDS             complement(15987..16805)
                     /locus_tag="ACG8XC_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFP9190476.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8XC_000758"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8XC_000758"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9190477.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8XC_000759"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8XC_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP9190478.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8XC_000760"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8XC_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP9190479.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8XC_000761"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8XC_000761"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP9190480.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG8XC_000762"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG8XC_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFP9190481.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="ACG8XC_000763"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="ACG8XC_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP9190482.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG8XC_000764"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG8XC_000764"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP9190483.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG8XC_000765"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG8XC_000765"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFP9190484.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG8XC_000766"
     CDS             26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG8XC_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP9190485.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="ACG8XC_000767"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="ACG8XC_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9190486.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG8XC_000768"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG8XC_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP9190487.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="ACG8XC_000769"
     CDS             28113..30086
                     /locus_tag="ACG8XC_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFP9190488.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="ACG8XC_000770"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="ACG8XC_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP9190489.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG8XC_000771"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG8XC_000771"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP9190490.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="ACG8XC_000772"
     CDS             32965..34257
                     /locus_tag="ACG8XC_000772"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP9190491.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10496 a         6691 c         6807 g        10403 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatacctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga ccaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta
    25201 tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga
    25261 agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa
    26641 gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattggatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca
    34381 cagtcccttt ttccgaa
//
LOCUS       DAZKCY010000018        33893 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000018 DAZKCY010000000
VERSION     DAZKCY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.018"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..901
                     /locus_tag="ACG8XC_000773"
     CDS             71..901
                     /locus_tag="ACG8XC_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFP9190492.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(1145..1394)
                     /locus_tag="ACG8XC_000774"
                     /pseudo
     CDS             complement(1145..1394)
                     /locus_tag="ACG8XC_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1585..1779)
                     /locus_tag="ACG8XC_000775"
     CDS             complement(1585..1779)
                     /locus_tag="ACG8XC_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190493.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(2284..2469)
                     /locus_tag="ACG8XC_000776"
     CDS             complement(2284..2469)
                     /locus_tag="ACG8XC_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190494.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(3319..3660)
                     /locus_tag="ACG8XC_000777"
     CDS             complement(3319..3660)
                     /locus_tag="ACG8XC_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9190495.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(3660..3890)
                     /locus_tag="ACG8XC_000778"
     CDS             complement(3660..3890)
                     /locus_tag="ACG8XC_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9190496.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            4177..4515
                     /locus_tag="ACG8XC_000779"
     CDS             4177..4515
                     /locus_tag="ACG8XC_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190497.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(4720..5282)
                     /locus_tag="ACG8XC_000780"
                     /pseudo
     CDS             complement(4720..5282)
                     /locus_tag="ACG8XC_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(6014..6595)
                     /locus_tag="ACG8XC_000781"
     CDS             complement(6014..6595)
                     /locus_tag="ACG8XC_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9190498.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(6778..7404)
                     /locus_tag="ACG8XC_000782"
     CDS             complement(6778..7404)
                     /locus_tag="ACG8XC_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP9190499.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(7426..8757)
                     /locus_tag="ACG8XC_000783"
     CDS             complement(7426..8757)
                     /locus_tag="ACG8XC_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFP9190500.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(8912..9313)
                     /locus_tag="ACG8XC_000784"
     CDS             complement(8912..9313)
                     /locus_tag="ACG8XC_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP9190501.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(9399..9605)
                     /locus_tag="ACG8XC_000785"
     CDS             complement(9399..9605)
                     /locus_tag="ACG8XC_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190502.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(9700..11202)
                     /locus_tag="ACG8XC_000786"
     CDS             complement(9700..11202)
                     /locus_tag="ACG8XC_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFP9190503.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(11220..11675)
                     /gene="argR"
                     /locus_tag="ACG8XC_000787"
     CDS             complement(11220..11675)
                     /gene="argR"
                     /locus_tag="ACG8XC_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9190504.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(11773..12390)
                     /locus_tag="ACG8XC_000788"
     CDS             complement(11773..12390)
                     /locus_tag="ACG8XC_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9190505.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            12611..13984
                     /locus_tag="ACG8XC_000789"
     CDS             12611..13984
                     /locus_tag="ACG8XC_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9190506.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLVSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            14059..15171
                     /locus_tag="ACG8XC_000790"
     CDS             14059..15171
                     /locus_tag="ACG8XC_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9190507.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(15243..16880)
                     /locus_tag="ACG8XC_000791"
     CDS             complement(15243..16880)
                     /locus_tag="ACG8XC_000791"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP9190508.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(17048..17656)
                     /locus_tag="ACG8XC_000792"
     CDS             complement(17048..17656)
                     /locus_tag="ACG8XC_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9190509.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(18040..19191)
                     /gene="queG"
                     /locus_tag="ACG8XC_000793"
     CDS             complement(18040..19191)
                     /gene="queG"
                     /locus_tag="ACG8XC_000793"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP9190510.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(19210..19962)
                     /locus_tag="ACG8XC_000794"
     CDS             complement(19210..19962)
                     /locus_tag="ACG8XC_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190511.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(20103..21053)
                     /locus_tag="ACG8XC_000795"
     CDS             complement(20103..21053)
                     /locus_tag="ACG8XC_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP9190512.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(21054..21893)
                     /locus_tag="ACG8XC_000796"
     CDS             complement(21054..21893)
                     /locus_tag="ACG8XC_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9190513.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(21890..22636)
                     /locus_tag="ACG8XC_000797"
     CDS             complement(21890..22636)
                     /locus_tag="ACG8XC_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9190514.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            22874..24244
                     /locus_tag="ACG8XC_000798"
     CDS             22874..24244
                     /locus_tag="ACG8XC_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9190515.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(24299..25195)
                     /locus_tag="ACG8XC_000799"
     CDS             complement(24299..25195)
                     /locus_tag="ACG8XC_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9190516.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(25309..25479)
                     /locus_tag="ACG8XC_000800"
     CDS             complement(25309..25479)
                     /locus_tag="ACG8XC_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190517.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(25476..26153)
                     /locus_tag="ACG8XC_000801"
     CDS             complement(25476..26153)
                     /locus_tag="ACG8XC_000801"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9190518.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(26244..27149)
                     /locus_tag="ACG8XC_000802"
     CDS             complement(26244..27149)
                     /locus_tag="ACG8XC_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFP9190519.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTGESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            27324..28778
                     /locus_tag="ACG8XC_000803"
     CDS             27324..28778
                     /locus_tag="ACG8XC_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFP9190520.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPTLSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVASASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            28794..29825
                     /locus_tag="ACG8XC_000804"
     CDS             28794..29825
                     /locus_tag="ACG8XC_000804"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9190521.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            29832..31019
                     /locus_tag="ACG8XC_000805"
     CDS             29832..31019
                     /locus_tag="ACG8XC_000805"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP9190522.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            31175..32557
                     /locus_tag="ACG8XC_000806"
     CDS             31175..32557
                     /locus_tag="ACG8XC_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP9190523.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            32529..32915
                     /locus_tag="ACG8XC_000807"
     CDS             32529..32915
                     /locus_tag="ACG8XC_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190524.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(33115..33783)
                     /locus_tag="ACG8XC_000808"
     CDS             complement(33115..33783)
                     /locus_tag="ACG8XC_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP9190525.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         9689 a         6858 c         6108 g        11238 t
ORIGIN      
        1 ctttctttga attttttaat ctttcgtagc agaatgagaa tgtattaaaa atagattagg
       61 aggaattttg atgtttagac accagaaaga attgcagttt gaagcaaaac cagatcgtcc
      121 gaatccatta atcgcgaaat atctgcagga gctgatcggc ggccaatatg gcgagatgag
      181 tgtagctatg cagtacctgt tccaaggctg gagctgccga ggagaagaaa aatataaaga
      241 tatgctgatg gacattgcaa cagaagaaat cggccacgtg gaaatgttgg cgacaatggt
      301 tgctcgttta ttagagggtg cgcctgttga agatcaagaa gcagcgatgc aaaaagatcc
      361 aacaatcggt gccattatct ctggaatgaa tccacagcac gcgatcgtag ctggcttagg
      421 acctcgtcct gctgacagcg tgggcaatcc atggagcggt gcctatatca ttgcgagtgg
      481 caacttgtta gcagacttta gagctaattt aaatgccgaa tcacaaggaa gacttcaagt
      541 agcacgtatt tacgaaatga tcgatgatcg tggcgtaaaa gatatgctgt ctgttctcct
      601 tgcaagagat agctatcatc aaaatctatg ggaatgtgca gtgaaagaat tagaagaaaa
      661 agaaggttca atcttagtcc caagtacttt cccgcgtgac aaagaacgaa aagacattgc
      721 ttatgacttc tataacttct cagagggaga ccaaagtagt gaaggttctt gggctagtgg
      781 caaagcactg gatggcgaag gcgaatatca atggttgaaa gaacctaaga tcgaaggcgc
      841 tgcaccaaaa ttagaaccag taacaccaaa aatgtatggg acacctccat tcaagaaata
      901 aatatctacc cgtagaaaca cccgtttttt gttccacacg ctttgtgagc aaaactgact
      961 aaagtcatta atcataacca cccttcaaaa gggtggtttt ctctagagct ataagctcaa
     1021 atcaccggcc agcgcctaaa gacgctggct ttcatatttt atgttcaagc catattgcct
     1081 ttgccagttt tgcccacctc ttaaaaggtt tatgtactac tccttagccc ttaaaaggat
     1141 cgtcttactc tcgtacactc agcttatcta acgcactatc atgtttttct tgttcttgaa
     1201 tgtacttttt gatggtcgct tcattttgtc ctacttttgt cacatagtat ccttccaccc
     1261 aaaagtgtcg atacccaaat ttatatttta gatttgcaca atctcttgat gatctcctga
     1321 atattggctc gatattgatt ataaattatt tttcttctat actttggtgt gaatacaatg
     1381 tggtatttac acatctactt tgtacgggct aaactatttc cctttattgc tccacccgca
     1441 tagcaagtgg ttttgtattt caatcgctcg gcgcgctcaa ttaggtctaa agacaataaa
     1501 tctaaaaacc cgctgcttca ctgacagaaa caacgggttt tcagaaaaaa atgaaatgaa
     1561 aaagatctct tacggcaatc tattttattt ttttgatttt ttataaatgg aaaatgcttc
     1621 aagaagtcca agaaataaag ccgtataaaa acaagaacct atgaaatctt tatttattag
     1681 atattcaaca gaaatcccga taacactagc caacaatata tttatcagaa gtgtaaaata
     1741 aggatgcttt ttatagaata gaactagtgt tgttttcata gactctcctt tcttcatatt
     1801 tccaattaat cacaaattca cggcattact agaaaaatta aaggggcgca gaagtggaaa
     1861 aatactcttc gactttccct acaaaccagg ccatcttgtg tttaataact ataccaaatc
     1921 taaggtacct ctaaatctaa attccatttg tggcggtgac ctccaatttt taactcttct
     1981 atctcccatc cagtatacat aaccttgata cttcgaccac ttatatatcg aacacacaaa
     2041 tacctatcca ttgcaggagt actacctaac ttagttgcta cttgcatcta aataacagct
     2101 gaaaagatag gacttactct tagtaaagta tctgaactat taatttttaa actatcattt
     2161 ttatcctcaa ctacatgcac atctgttgca acttcacttt ctgtctcatc cagataaatg
     2221 attaaggaat cgggaattgc aatatcttca gctgacgctg gaatagttga gctaaattag
     2281 tcatcatatc tttattataa aataaccaac agcaaaaaat acaatgaaaa acaaacttgg
     2341 aactaatgta tcattaataa atgttgaaaa attcagcaac tcattactgt atgattttag
     2401 cagcccaacc aacatatacc ctataaaagc taataagatg gttaggaatc ctttagaatt
     2461 tttactcatt catagacact tccttaaata acttttatta tagaaggtat tgtaaataat
     2521 aatattgaca aaaagtgact caaaataaaa aaaaacttac attatactgg aatgaactgt
     2581 attgatagtg tacctaaaag ctctactgac ataagatcgt gtaatcggta aagtaatttt
     2641 attaattata cgggatgtaa tcaattagag aataaactaa tcataaaagg tacttttaca
     2701 ttcataattg tcgctttgag attctacttt ggttcactat ctgacccaat tgcacatttt
     2761 cgatcattac aagtattggt gtcgttagaa caactaaata aaaactagcg gtagattttt
     2821 cagaatctat gctaatttct gtatggatgt tccagtgatg gcaaatttct cttttcattg
     2881 tttttcttgt cgcatgatat gcctttttgt gttattcagt taaactatag aaattctttc
     2941 ctagacagat cttgatccca aattttacgt ttttgagcat cgatatagtt taaaatgaca
     3001 ttactcatat ctaaagagtt tagatttttt aggactaaaa ctgtcagttg ttctttttct
     3061 tctttctgtt aaagtattgt cgcgactttt cgtatacgtt aagatatgtc gtgaaaaaac
     3121 aagttttgtg acacaaaaaa acgaatgact tgaggaaatc acctcaatca ttcgtttaaa
     3181 atgctatata ttctttagtt taagatacgg aaattaatgc catcgaccac actagtagaa
     3241 tgaacgacat ttacttggcc tttttttaat ttcgttatgt ttagcatata aaaatattat
     3301 agagtttact tacaatgctt aaaacatata ctgtataatc tgatcaattt tagccatgtc
     3361 ttgcaatggt aagttttcta tttttttgag ttttcgttcc ttaaaatcaa gagattttaa
     3421 ttgtgtaata attacttgtc cttttatatc tagctcttca ggtaatgagt aacgtgtcgg
     3481 taaattcttt atagtggaag taattggaca tatgaccgaa aacattgttt tacggttaaa
     3541 ttcataacga gagacgacta gtcctggtcg tcttttttga atttcttttc ctacggaggg
     3601 atcaaaatca atccatacaa tatcaccttt tttaggaata taaattcttt cttctgacac
     3661 tataaaattt ctctcccttc aggagttaaa tcttcccatt cgtctttctc ataatactcg
     3721 gcctcttccc caccactaaa tggatcttca atttttggaa ctaacgtaat tgttccatca
     3781 tcagaataca caacgatata ttcttggttt tctgaaggtt tctttccgtt atcagaaggt
     3841 aaagttacca ctacagaact tccttgtaat ctagattttg taacaagcat tatcatcact
     3901 cccttaataa aagtatacta tataacacta agtaacgcaa aattcccctt tgtaaactat
     3961 ctagaaaatt gttttgttta aagtatatct tattggatat aaatagaaat aaattgtagc
     4021 cagttcattt tttctttaac taaaaaaata atccaagaaa cattgaacct atggatccaa
     4081 aagctaccca aatactatag ctaacccccc gctaccttca attttgcaga taaaacgctt
     4141 tgggagaaaa tttcaaagac aacaggccat cctatcatgc ccgattataa acaaaaagct
     4201 ggctattcct tttgctctga caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa
     4261 gaagtttcaa ctgttcggat caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga
     4321 atttcaacta gacaagactt tgctattgta gaacgtgaga tcgtagctat ttttgtagaa
     4381 gaagggattt tacatgatcc aactcatatc aatgtggata aatggcaacc tcttatctat
     4441 aaattcagag aatatacaac tgccaaccag tcgttaactt tgaattttag attccaagaa
     4501 ttcaaagagt cataatgata aacatcgtta ggtataaata aaaaagactc gcagcacgag
     4561 taaaaaacaa atagaagcta tacattagaa aggtcgactg tgaccctata gatgcagtat
     4621 atgcagctta tgagcatttc ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt
     4681 ttcgtttttt gccgctcttt ctttgaaaaa tcgattatgt tataccttct ccattactaa
     4741 ttcaggcatc tgctgagtaa tgatttttcg cgcaaaagaa atatctgtta cccaaggttc
     4801 gatttgttct tcagtcaaga caagcggcat tcggtcatgg attggtgcga ctgtgcggtt
     4861 aggttcagta gtcattatga ttgattctgc cattttttca tcctgatggc tacgataaaa
     4921 accgccaata tacactattt tttccttatt tgaaaacata aatttgtctt tttcttcatt
     4981 ccactcattg ggaaaacaca acgattttgc aaaaatggtt gatgaaaggt ctttttttct
     5041 tctactgtct ctgcacgggc attaatcatc aattgtcctt ttttaaatcc ggtaaatccc
     5101 cacttcatga tttccggagt gaggatgttt tcttcctctt ttcttaacgt cacaacaaga
     5161 tttgacggaa aaacttctcc agctgctaat tctgctgcct gcttcggttg tttcgtctgg
     5221 gcttctcgat aataattctg aaggagctct gactctagat caaaaaaata acgaccacac
     5281 atcctaatcc ctcccaatct ttttttctat catacactgc aacaagaagt tcgcaccaac
     5341 aaaacgcttt caatttttgt agaactaaaa aagttgtgac aagtgacaaa ctctccagtg
     5401 aatggtattc tcctgccaac tcctcggtca caagtcacaa tattcaacaa acatgagaag
     5461 ctgctttttg aaattgttcc ttgtgactcg gagtgaaacg gcaggcttac gacctctggg
     5521 taaacggtgt tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtagaa
     5581 agctattttc acaaaatttt tcttaatact cgaaactaag cgactttttc acaacctctt
     5641 ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
     5701 gagcagctat ttttatttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
     5761 tctccactaa acgatgttca atcagctaac cttcggtatt agctcaaaaa acggcaaaat
     5821 atgaggaact attttgccgt ttgtttctct aatactcagg tcaaagcgct gatttcacaa
     5881 cctctaatac acggtgttcc agaagtagct tcttcgaaaa ataagccgaa atttcacaaa
     5941 aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca
     6001 caacctctaa ttattaaaga accttttgcg aagttaacaa tctttctttg atcaattgaa
     6061 aattctgcct aagtagtgat agatcctgtg tgactttcaa caataagata cttccctgga
     6121 tttcagcaat caacgctttg gcttgacggt ctgcttcttc atcggacagt ttttcttcag
     6181 aaagtgccag gatattttgt ttcgtatact tgacccattg attgactaat tctagtagca
     6241 acagacggta ttcttcatcc actgtactca actcaataat caaattacct acaggacagc
     6301 catgataagt gatctgtgtc tcatgatcag cgaaaatcca gtccaacatt tcttctaatg
     6361 cttcttctgg ctgttttcca gcatttaaga tgtcttcgaa caattctgtc tgccatacgt
     6421 ccatatgcgc atggatcacc gctagacaaa cttctttttt tgaagaaaag taataataaa
     6481 attgaccttt tcctgtatca gaagctgcta gaatatctcg gattgacgtt tcatgatagc
     6541 cttttttata aataagatct ttggtcgctt ccaagatctt ttcctttgtt ttcaatgata
     6601 ttctcctttt tcatttctca tagtcctata ttatacagta aattcatcat tttttcttct
     6661 taatcgttta aaaaagactg tgccaaatta tctgacacag ccctcactat aaaactgttc
     6721 tcatatagct agatgttcat tgattcaaga actgcgagga caaaagcaat tcctatctta
     6781 tgatagttgc tctaatactt cctctgttgt catcacttga tctgcaataa agccaaagtt
     6841 cgtcaacgct gcatccattg cttcttggcc aggagcacca attgcatctg tcgctaccac
     6901 aacattgaat ccactctcta ccaattcacg catatgggaa tcgctacaca agtttccgtt
     6961 cattccacca agaattactg tgtcgattcc attttttctc aattggagcg ccaaatcatt
     7021 gctttctggt ccaaagattt tatgaggaga gctgatgatc gtattttcat ccaaataagg
     7081 tttcaattct tcgataaaat cggctcctga accttcttcc actgattcat attgactttt
     7141 tctagcaaac atctgctgct caagcatcat atgttctcct gcaccgccaa attgccattt
     7201 atggtcatgt gggtaaaaat aatgaggaga aataaagagt ttgtatcctt tttcttttgc
     7261 ttgttttacc atttcaatca tattttcgat cgtattgtat ttttctaaga tatcttttgt
     7321 caaaccaaag ccaccgcctg ctggtttcaa gaactcgact tgcgggtcag tgagaactac
     7381 tgctgtattt ttattattga acatggtgga ttctccattc gttatttatt ttaatacatc
     7441 atttagagct tcactcattg tcggatgaga atagatttga tctctcaata acgtataagg
     7501 aagttttcca ttcatagcta gcgaaatcaa gttgatgact tcatgagaat cttccgcata
     7561 gatacttgcc cctagaatca agtcagtttc agggtcgact aaaactttga atacaccttt
     7621 aggatcttct aatacttgtg ctttagggac accgcttgtc atatacttga ataatttgta
     7681 attgacgcct tgtgctttgg cttgtttttc tgttaaacct acattggata aagtcggagt
     7741 gataaataca ctgtaaggca cagctttccg gttattcgtt gttcgtttat tctcgccctt
     7801 caactgatcc ataatgattc gataatcatc tagagagata taagtgaact gcaatccgcc
     7861 tttgacgtct ccaatcgccc aaatgttgtc tgcatctgta cgaaggtatt catccacagc
     7921 gattgctccg cgatctgtta gtttaacatc tgtattttct aatccaagtg atgctgtact
     7981 tggttttctt ccagttgcag ccaaaacttt tgacgcttgg attgttactt cttggccatc
     8041 ttctgtaaag gtgacggcag ctgttgattc ttggtctgct acttgatcta cagatacacc
     8101 tagatggaat tcgattcccg catttgtcat gtcttctaaa atcatttgcg cgatatcttc
     8161 atcttcacgt ggaagaaatt cagaatgggc atcaagaacg acaactttag atccgtattc
     8221 attgaacatt gatgcaaatt ccaagccaat atatccagcc ccgatgatca ctaatttttc
     8281 cggtaattgt tcctgatcca tcgcttgcgt agaatcaatc aagtagcgac ttgtattcaa
     8341 tcctttgatt ggtaaaatga ttggttcaga accagtattg atgaaaatcc gttcgccttc
     8401 aacaatcact ttttcaccat tattcgtcac ttcgatttga tggttagaaa gaaaacgtgc
     8461 tgtaccgtcc atcactgtcc cagttgcctc atcgctgatc atatgataat ttttggcgcg
     8521 aagcatgccc gtcaattttt ctttccgagc aacagcctct gtaaagtcga ttcctctttc
     8581 tccattgaaa atcaatgatt tagaaggaat acagccgatg ttgatacatg ttccgccata
     8641 catacgtgtt gacttttcaa cgactaaaac cgattcacct tttgaagcta acgccttcgc
     8701 taaagtctta ccgccctttc caaaaccgat cacaatattt tgatattttt ccatcatttt
     8761 tccctcgctt cttttttacc taccgattgg tacaaaaata tgataaccga taattgtcta
     8821 ttctccaaat catctgcttt atttgtcaaa aaaaaccaga caaaaagcgg tcctttcacg
     8881 tttttagtct ggttcgattt cctttattct tctaaacaat cgttacttct acatcgatat
     8941 ttcctctagt tgctttggag taagggcaga attcatgcgc atcttctgca attttttggg
     9001 cttcttcttc gctgatgcca tcgatatgca cttctaatac tacaccgatc tgatagccgc
     9061 catcatctaa attataaaga gagacacgtg cttttactgt ggactctgct gtcattcctg
     9121 cttctttgat catatgttgt accgccccat tgaaacaaga agcataacct gctgcaaata
     9181 attgttctgg atttgttcct tctgcatgga ttcctggagg agtgattttc atatgcatcg
     9241 agccatttgg tgcctcgact tctccattgc gcccgccatg gttgatcatt gctgtttcgt
     9301 atgctttttt cattgtactg ctcctttcga ttgaccattt gctacttggc tataattata
     9361 gtgtatgaca agtaaggcgc tttctacaaa aattctgctt attcgaatac cagaatgccg
     9421 tcttctacta tttttccaaa aaacaaaaaa atactactcg tgaacaacag cgtcatattt
     9481 gttccaaaat aaatagcaat cttttctaca taatctttta tatgattgat tttaagatac
     9541 atatatacca gaaagttgac cccataaacc aatactgtta aaaaaataac catattcgtt
     9601 accatttttt cctccttttg ctggtctccc taccagcact cgatttaacc ataccacttt
     9661 ttttgccgtc caagcaacta gcctttcaaa aagactggtt tattctgctg ttaatgattc
     9721 aagatcagaa aaatgtccga ataattttgc taattttact aacgccaact tgttctttcc
     9781 gaattcatca ttttctatct gtaatactag taacggctca tgtaagctta aacggatcaa
     9841 aaaccagccg tttccgtatg gctcttctag atcatatcga ataccttctt cattttccgg
     9901 gtcgaagttc atcccttttt cttcttccaa cagtcgcttc atttcttcta atatcttgtt
     9961 tccttgttcg cgtggttcat cgctgaacaa ttttaatcgg atttcttgta cttctacggg
    10021 ctgtttcaac tctttgattt gttcatctaa tgtgatacct tttcttttta attctggcaa
    10081 aagcatcagg atctttgcga tgacatatgc accatcatcc agaaaatagt tttccttgaa
    10141 tgcagcgtgt ccgcttgttt cgatggctag ctgtgtatct ttcccaagac ggttaagttc
    10201 taatgcttta ttgattacat ttcgatagcc ggaaatataa cgaacttgga tgcctccttt
    10261 ttcttcaatg aatctttgta gatgagaaga agtaggggaa ttcgtcacga ttcttgttcc
    10321 tggatgctct tttaatacga ttgtacttaa tactgcgatc aaattatttc gattgatcac
    10381 tgttccagaa gcactgacaa ctgcagaacg atccacatct gtatcgaaaa tgatcccaag
    10441 atctgcttca tttgctaaaa cagcctgctt gatactttcc atcgcttctt tattatcagg
    10501 atttgggata tgattaggaa atcggccatc tggttccaaa aattgggaac ctgttgaatc
    10561 cgcacctaag ctattcaata ctttttcagc aaagaatcca ccagcaccat ttccggcatc
    10621 aacaatgatt ttccaaccat ttagtggttt gctttctagt gttccaatcc cctttttaat
    10681 ttttgtgact aaatcttgcg cataaggtgt caaaaggtct gctttttcta cgcttccagt
    10741 ttgttcgctt tttatttttt gctgctgtga taggataaaa gcaatatctt ctttttctgc
    10801 tcctccttct gaggtaaaga attttattcc attataataa tagggaagat ggctagcagt
    10861 cagcataacc gcagcatcgc acgagaactg ttcaaattga gtggacataa acatagcagg
    10921 cgtagtagct aattcgaaat cgatgacatg tatcccttgt tctttcagac tttggatcaa
    10981 cgcctctttc agtgctggtc cgcttatcct actgtcatga ccaatcccaa tcgtccattt
    11041 tttatttgga tcggttgatt tcttcttttt ctttatccag tttgctagtc cccagccgat
    11101 cttttttacc atgtctgtcg tcaagtttac ttcatatcct tcaactggta atgcgattcc
    11161 tcgaatatcc gatccatttt gcagcgcttt catatctttc atataaaagt cctcctcatt
    11221 tattccctca ttttttccag cttttctgca aatcgctgtg cttcttcttc tgagcggcaa
    11281 ataataatga atgtatctac accggccatc gttccgacaa cttcagggta agccacttca
    11341 tctagccaat tcgttaccac atcggcacca tttcgttcag tcaatacgat aaccatgaac
    11401 tgaacagact ggatacgcaa aacctcgcgt ctaattgctg tacgcaaacg ttcttcattt
    11461 aaagaaacag atggctggtt aaataatgta tagcgtacta ttttgttttc attttgactt
    11521 tttactaact tcatttctcg aatgtctcga gaaatcgttg cttgagtcac atcggcgcct
    11581 gcttcatgca gccgcgcaag cagctcttcc tgtgtcccaa taggttgttc tgtaatgatc
    11641 tggcggatca gtgcttgtcg ctgtttcttt ttcatattag acacctctca atgttgatta
    11701 tagcaaaaga taagtaatat ttcttcatat tttggtattt tttactttta ttttttgttt
    11761 tcattcaggt tgctaacctt tttctatttt aacacgcaaa atcgtcttca ttttttcagg
    11821 tgctgatttt cttggatcgc tgatgtaaat ctctttgtgt tctttagaag ctctttcata
    11881 gccttctttt gcgatgaatg cttctaattt ctcaaacgtt gctggttctt cggcataggg
    11941 cccgacgtgt aagatttggg cacaaagacc ttcttccatc tttttgaata ctacttgttc
    12001 tttcaagtct tccggaagtt tgctagaacg ctgaatggct tcttgtgcta tcgcttctgt
    12061 aacaaagtca ggctgtcgga tcatcaattg ataagaaaaa tgttcttttt tcatcactgg
    12121 ttcatgcaaa tacttttctt gcagtcccca ttgtccttcc agtggataga cagtatacat
    12181 ctgataatca ggaatcagcc aatttttttt aggcgacatg cgtattgcat agctgactgc
    12241 atatagacag gctactcttc gtttaaagtc ttcctgattc gggtcgccga tccctctgat
    12301 gctaaagaat gattttttag gaacctctag tatttgcggg ctttttttca tactatagct
    12361 cttgtcttta cgccattcaa ttttttccat ttttcttcct ccgcgaaatt ccatttagtt
    12421 atttattatc atacctcttt ttgagtgact tggctaatcg tgttgacact atttttcagt
    12481 ttgtgctaga ataagtgaag taaataaatc gtacagataa cacgtttcgg cgtgtcgaga
    12541 gagaggcggt ttctattttt ataatagaaa tcactctctt tttttacgaa attttttaga
    12601 ggtgatgtaa atgacaaaac aaaaacagct gcttttagta cctgcagtaa ttgcaacagg
    12661 gatgatgtct tttgcgggcg tattgatcga gacggccatg aatgtaactt tcccaactct
    12721 tatcagacag tttgatttaa cgactgcaca agtccaatgg gtaacaacga tttatctttt
    12781 gatgatttcg atcattgttc cgctgtctac ttatttgaat cgcaatttta gtctcaaaaa
    12841 actcttttta gcttcaaatc tgcttttctt ggcaggtata atgatcgatt tcttctctcc
    12901 taccttcatg gtcctattgt ttggtcgatt gctccaaggt gcagcgacag gtatcgctct
    12961 tccgttgatg ttccatatta tcttgacatt tgcccccctc aatcgacgag gcgcgatgat
    13021 gggtgtcggt actttgacaa cggccattgc tccagcaatt ggccccactt atggaggcat
    13081 tatgacttca cattttacat ggaatcatat cttcctgttt cttgtgcccg ttttgattct
    13141 ttctttgatt atcggctggt ttgctattcc cgagatggca gtacaaaaaa gtggttattt
    13201 ggattggata agcgttttag gaatttgtct tttgtttagt ggaagtctaa catttttgag
    13261 tacgttggga acagtttcca gctggattgc tttaatcatc ggcggtattg gctggttcct
    13321 tttttactat cgaagtaaaa aagtggagca tccactgatc cgactgtcta ttctttcaaa
    13381 taaaacattc cgcttgtttt tgattggctt cttagtttat cagtttttat tacttggtgt
    13441 atcttttgtc ttgccgaatt tccttcagat cgttcaagga agtagtcctt ttgtcgctgg
    13501 attagcgatg cttcccggcg cagcggttgg tgctttgctt tctccttttt ccgggaaaat
    13561 gctggatcaa tatggtccga agaagccaat ttttgctggg ctgattcttt ctttgattgg
    13621 ttggctagca ttattcctac tcataggaca cgcttcagtg ggtgtattga ttgcttgtca
    13681 tgtgttttat atgatcggta tcggattatc ttacagcaat atgatgacca ccggaatgaa
    13741 tgcactgaat gaagaactac aaggagacgg gaacgcaatg ttcaatacct tgcagcagtt
    13801 ttctggcgct gtggctactt cattagtagc ggtcattatc aattacgtcc agcatcatac
    13861 ttcccataac tatgaagtgt ctactacatt agtatcaaaa gcagcattag gcgtcttgtt
    13921 gctgttgctt ctcgtaagct ttgctcgatt cgcctattat ctgttctttg ctaaaaaagc
    13981 gtagattcca tatacggcag gtgttgcatc tgccgttttt ttgtgtgaaa atggttgttt
    14041 gtaaaaggag gcttttttat ggaaaaaatt gccattatcg gtggcgggat cattggtatg
    14101 accctagcca gttacttaga tacatcaaaa ttcaatgttg ctttatttga tgaagggaca
    14161 ggacaggcaa ctaaagctag cgctgggatt atctcccctt ggttgtcgaa acgcagaaac
    14221 aaacaatggt atcgtctggc taaagacggc gctgcttttt tccctaaact tataaaagat
    14281 ttccaacttg atcaaacgat ttacgcacaa tcagggacac ttttgcttcg acctgaaaaa
    14341 gatttagctg aattggctgt tcttgccgag aaacgaaaag aagaagcgcc agaaattgga
    14401 aacatcgaat ggctttcacc tgaacagaca tctcaagcac ttccgttatt gaaaccagca
    14461 gtttcactgc gtatcagtgg tggtggtcgt ctggatggaa aagcatattt agaacatctt
    14521 ggtcagcgtt tagcacaaaa acaaatggag cagatccagg caaaagtcac tttgcataag
    14581 aagagcgatt cttggctcat caagggaccg tttggagaat acacagctga tcgtgtaatt
    14641 ttgacacctg gcccggcact taagccttta ttacaagaat taggctatgc tgcagatata
    14701 cgtccgcaaa aagggcagct cttagcattt cagacatctt ataagatgag tcaagactgg
    14761 cctgttgcta tgcttgacgg agaagctgat ttcattcctt tcatggatgg aaagatcctt
    14821 ttaggtgcta cccatgaaaa cgatcaaggc tgggatttgg ctcctactag cgaagcttat
    14881 aagggattga ctaagagtac agaacagttc ttgactgatc cagatcagat aaaaaactgg
    14941 ccatacgagt tccgtgtagg tacccgtgct tatacgtcag atttttcccc atttttcggc
    15001 gtactgccag atgatccaag cttactggct gctagcggtc taggctcttc tggcttgacc
    15061 acaggtcctt atatcggcta tctattagcc aacttcttaa atactggtca ttggcctacg
    15121 gaagattatc aaaaggacat ctccacctat attacgaaaa atccatcata gaaaaacagc
    15181 tttttcaagt aaggatcccg agcgatattg cgcatggatt acttcttgaa aaagctgtta
    15241 ttttagttca atttctgaat caaattctcc aaactttctt ttgcttcatc tggcaagaga
    15301 tcttttccta tctgagcttc aatgaatcgt aaacgaatat caagtcgttg tttcaccaga
    15361 tttttatagg acgtatcctc aaaagaaaca ggaaaatctg gtataggtaa atagtggatg
    15421 tcttcaaatg gaccaaacga cttgaattct gccgtttgtt gcacgattct ttcaacgatt
    15481 gacaatgtcg tttcaggttt gattttttta tcatcgaaat atccagtatt caggacatag
    15541 caggctgttt gctgtttcaa aaacaactct cgaaaagctt gatagtcttc cctaagagga
    15601 tagacacgga aaggattagc aaatggttca atgactagct catctagctg tctttttgta
    15661 gtgatatttt ccgcgtctga tcgttttgtt gctaatgttg caccaaaaga cgctgctaaa
    15721 actgggtcat tgatcttgat aattggcgga agcgaatcat ctttcatgat ccaaaaaaca
    15781 gcatcgatct tttcgctaag atgatcgact cggtcgggtg tcgcataacg tgatttgact
    15841 gtccgcccat ttccgtttcg tatatctcct gttactagga ctttttctcc tttacttgta
    15901 catgtcacgc ccacattctg acaagtaagg aaagaagaaa tggccggatc atcagacgga
    15961 tagtcttgtg ttttatcaaa gtaagaaggt tccaaggcag tagcaaaacc attttttcga
    16021 gaaataacga aagcatcgtc atgcaagatc gaaacagagt gaccttccgt atgttttgct
    16081 aaggtaatcg ttgattttcc agaaccggac aacccaaata cagccatcgt ataggtctgg
    16141 tcattactag catattgttt catcccaccg tggcaggcaa taaagccatt gcgatttccc
    16201 aatgcccaag caagcgtcaa agtcgctttt tttaattctc caaagtaacg taatcctagc
    16261 acagccgctg tattatggag agcatcaaac aaaatgagtc catttggaaa tgtttcatgt
    16321 gtccattcgg gatcacaaaa gatataaaga tctccctcat caaaggaaac agacttttga
    16381 taagtctcta agatcttcga attcgctggc tgaaaattca gcaaataaga atacaaattg
    16441 ttctcaaatc cttctggaat cagcaaatga ctttgcacag aaaattcttc agataggcca
    16501 acgattactt ttgtatggta aaactggcgc ttgcgacttt gatacacagc ttctcttaaa
    16561 attttctcgt atttgctttg ttccttgtct aagtcctcaa agatccgccg agcacttgct
    16621 gttcttccca caatgcttcc atcattgaat actagtgtcc taacatcagc tggtaaatct
    16681 agttcttcag catgtgagat tgttaaatct gtgatgatcg ttcctggact cccttttgct
    16741 agttgataag ctgtcttcct atcaaaaact tcatgaacat tattaccgta aaatgtcgtt
    16801 tctacaatcg atcgaatact ggataacagt ggatttccac ttgtgatttc gtttaattcg
    16861 agactctctt ttgtactcat ggctcctcct cattccctaa acaaaaaaac gttttcttta
    16921 ttatacatta ttcgagtttt tatttgtact gctgcgctgt gggaagcaaa cgagttattt
    16981 ttcacacaaa aaaaccgaga acttgaataa acaagtttct cggtttcatc attttccttt
    17041 tatcggctta gtaccagccg tttgctaacc agaatgtttt agcagcatcc catgaaccat
    17101 aacgagatgt tacatattgt tctgctacac gttcttgatt ggcagctgaa tagtcgccgt
    17161 ttaagtatga agcatctaat tgataacggc cgatgtaacg tccgttagta gctgtgtatg
    17221 aaccgcctga ttctttttgt gcgatccatt cttttgcaga gcttgaagtt ggcgctgttg
    17281 cagcagctgg ggcagcagga gctacttcaa ctgtttcaac aggtgcaacg tatgtatgtt
    17341 cttgcgttgc tgtttcagtt gtagatgctg tattttctgt tgttttgttt tctgttgttg
    17401 ctgaagaatc atttgaagtt gggattgtta attgttcacc aacataaatg cggttgatgt
    17461 ttgcgatcga atttttttca gcaattgcat cgatcattga gttgtctcct gcaaattttt
    17521 gtgagatctt agataatgaa tcacctgatt ttactgtata aacttcatca gcgtgagcat
    17581 ttgtagttcc tacaaacatt gcagtagcac cagcagcaaa agttgtacct aaaacaattt
    17641 tcttaaatga attcatatta aactcctttt tttctccgtt ttttattctt tcgacgctat
    17701 taactttatc atttagaaat atttcatagg taaaagaaac atgacattta atgacaaaat
    17761 aacgaattat ttaatatttg taacatttaa acaaatattt ttttgataca aaaccataaa
    17821 agccttgttg tataagcaaa gtaatcgttt ttctatgttt ttttattttt gaatgaaaaa
    17881 cgcagattct gtaacattca ctgcaataat ccataaaaaa aagcaaaaaa atttttgatt
    17941 tttatcaaat tctcttaaaa aaatgtaaaa aatacaaaaa aaccagtaat tgttacaaca
    18001 attactggac aaaaactttg aataatgtta actatttttt tattttaact ttctccgcaa
    18061 taatttctgg gcatgttcca attcgtttat aacgacttct tctgtttctt tagctaatat
    18121 ttcatctaat acatcaagcc atttttccgg ttcttttgtt cctagacgtc caatagccca
    18181 agctgctgtt cctctgatga cagggcggac atctgttaaa cattctgaaa tctcaggcaa
    18241 tgccgttcga tcacctagat tagctaaagc aatcaatgcg tttctctgca aaggtttctt
    18301 cccacgccat gaaccagcta aatgcccaaa ttgctgtttg aagtctttat tagacatcga
    18361 aagcattggc tttaattttg gatatatttc gtctactttt ggttccatct cttcatgaaa
    18421 atgaaaatct tttccttgat tgtaagggca gactaactgg cagatatcac atccataaat
    18481 gacatttcgc atttttttac gatattcttc cggcatcatc ccttttgtct gagtttgata
    18541 agacaaacat cgctgggcat tcattcttcc gtcaccaagc aatgcttttg tcggacaagc
    18601 agtcacgcag cgcatacagt caccgcaacc aaattctcct ggtttatcag gctcaaatac
    18661 aatattagta acgatctcac caagatagac aaatgaaccg aactcttcag tgatcaacaa
    18721 tccgtttcgt ccaataaatc ctaaaccagc tcgctgtgct acagcgacat cgatcagttc
    18781 tccagtatct acttgcggag ctagacgcca ttcctcttct ttttgccatt tttctgctct
    18841 tgtttggatg aattcgatca atcggttcaa acggtctctg agtatatcat ggtaatcgat
    18901 cccccaagaa gcacgagcaa actgcccgcg tttttcatcc ctaggcactt cttcatggat
    18961 cctagtagga tacgcaagtg caatggaaat gatcgatttc ggctgatcaa atgtcatttc
    19021 tggataaaga cgctcatcga tatttggatg ttcaaagcca gaagtatggc cagctgcttt
    19081 ttgttcttcc aacgattctt ttaatgaatc aaaaggttcc gccgtggtga acccgatttt
    19141 gtcaattcct aatcgtttgc tttcttgtat gatttcttct tttaatgtca tggtcttcct
    19201 cgcttgctgt taacggctgt ataacccata atatagtttt tcttttttca gctggacaaa
    19261 ctggatctgc tgatcggcaa tcttcttttc ttccatccat ttttttcttg cagcaaatac
    19321 ttcccaataa tttgttgaaa ttaattttct tttgttatct aatgcattaa aaaaattcaa
    19381 tgaaactggt tttgtactta acagttcatg gagccttacc tcttcccatg gcgaaaactc
    19441 agacagatcg cgggaataca atacttcttc atagagattt ttttgctgtg tgtacgtgaa
    19501 tgtttgcggg attttcccag gcctagcaat ttctactaga cgattataaa acatattcat
    19561 taatttgctg ttggcaataa attgactttt ataataggcc gactggatct ttttgattac
    19621 tccttctgct ttgatgactg aaaataatgc aggccatgct ttgacggtta tttcgtcgtt
    19681 ttgtttaact aatacgccag gtgtgaagcc gactggagaa aggaacacaa aataaccgat
    19741 accggtattt tcatctaaag gctgaaattc atcaaacaac catttgccta tgtacagctc
    19801 ttcatctggt tctgttgtct cattttgatt tgctatgaat aatttagcta attctgcttc
    19861 aagaaattga aggtcttgat ccttgtcggt attttcctgc tggctgatca atcgaaagac
    19921 ttcttcttta atcgtatatt tatctttacg tgtaaaattc atagccacac cttccttcct
    19981 tactctaact ataacggaat ttcttcaaaa aaggtatctg tacgacgaaa aacagcataa
    20041 ttttttttca tatctcttat aaaaaatgca aggtctgctc ctcaagctag accttgcact
    20101 tttcagcttt cttttgtttg tgacaaacct tcatggatcg tttcaatatt ccatttcatc
    20161 atctgatagt aagaatctcc ttgttctcct tcttttgcta tggaatcagt gaacagttta
    20221 tcatagattt tcaaacccgt ttcttttgct acccgttcca tgcttcttga gtctacactg
    20281 gtttccacga ataaaaccgg tacttctttt gctcttatct gatcaatgat cgctttcatt
    20341 tgatcaggcg taccttgact ttctgtattg atctcccata tataagctgc tggcaaatca
    20401 tatgctcgtg aaaaatactt gaatgcccct tcacttgtta ctaataattt tttgttctct
    20461 tcgatagaag caaaactttc cttcgcttgt gtatctaatt cttttagttt ttccacatat
    20521 gcttctgcat tttttttata gagtgtcgca ttttctgcat ctttttcaga gaatatacgt
    20581 gcgatttcct ccacatattt tattccgtta gataggtcta gccaagcatg gggatctgct
    20641 tttgtatgtt cttcaccgct agttaaataa agaggttcta catttttgct aactgcaaaa
    20701 taatcttttc cttcttcttt tttagccgtt tccatcaagt tatcgaacca gctgttacct
    20761 gtttcaagat tcaaaccgtt gtataaaata acatctgcat cgcttgcctt tttgatgtct
    20821 tctggtagta cttcatattc atgcggatct gttccgactg gtacgatact gtggatatct
    20881 atttgatctg ttccgacctc ttttgccatg tccgccaaga tcgaattggt agccacgact
    20941 gacaactttt ctttacttcc ttcatccgct tggtcagaag cctgctttcc gcaaccggct
    21001 aataccaaga aactaactgc caagattaaa aagagtgatt tttttgtttt catctatctg
    21061 cttcctttct gaaataaaat tccttttgtt ggagaaaata aaaaagcaag gataaaaaat
    21121 gcggtacatg ctaacacgat cgtagctcct gatggccaat tgtaactata actgaaaaac
    21181 acaccgacaa ctgcacttag cataccgatt cctgcagctg taatgatcat tttcagtaaa
    21241 tggttcgtta gcaagtaagc cgtggcggct ggtgtgatca acatggcaat caccaagatt
    21301 gttcctactg tctgtaaact gactacagcc actaatgtca aaaagaacat caaaagataa
    21361 tggatcagcg atgtgttcag cccataagct tttgccattg tcggatcgaa tgatgtgatc
    21421 ttcaattctt tataaaagaa aaagacaaat atcaagacga ttccactaac gagtgctgtc
    21481 agcaacaaat cgctttccct aacagctaag acattcccaa ataaaatatg atacaaatct
    21541 gtcgcactct gggcatatga aatcaagata atccctaatg caaaaaagga gctaaagaca
    21601 attccaatcg ctgtatcatt tttcaatcgg ctccgctgag tcacgaaacc aatcactgct
    21661 gctgttagca tcccaaaagc agtggctcca aagatgtaac taaaaccaaa catataagaa
    21721 atggctacac ctggaagaac tgcgtgagag atagcgtctc ccatcaaaga catccctcgt
    21781 aaaataataa aagaaccgat gactccagaa gcaagtccta caagcatgga agtgaccaaa
    21841 gcgtattgca ggaattgata attcattaat ccatcaataa aggattggat cattttttct
    21901 ctcctccttt tactaaatgt ctgatgatct caccataggc caattgaagg ttcttcgatg
    21961 taaagacttc ttgtactgga ccggaagcga tcagctgttt atttaaaaga ataacctcat
    22021 cgaaatattc ttctacttta tgaagatcat gatggacgat caaaatcgtt tttcctgaat
    22081 tcttcaattc ctgcaagatg tcaaagatct ttcgttcact taacgcatca atccctacga
    22141 atggttcatc taaaaagatc cattctgctc cttgggctag agctcttgca ataaaaactc
    22201 tctgaagctg tccacccgat agttcgctga tctgtctttt ggcatattct tccatcccta
    22261 cttttttcaa agcttgtctt gcacgttctt tttctttttt cccaggtctt tgtccaattc
    22321 gtaaagatgg atatgttcct aaaagtacaa cgcctaatac atcaattgga aaggaaagat
    22381 ccaattcact tcgctgttcc acgtaggcta tttttttacg gactgagttt accgactgtc
    22441 cagaaaaggt gactttacca gtagtagctg gtatcaactc tagcaaagat ttcataaatg
    22501 ttgattttcc tgcaccgttt ggtccgataa taccggtaat tttttgtgga tttatcgtca
    22561 agttgatagt tttcaaaaca gttttccctt gataagcggc tgaaaggtct tgaattacaa
    22621 tggccgtacg ttccaaatct tacactcctt tttaggttta cctaactttt tatttaaata
    22681 tagtttaact aaaatggaaa gcattttcaa gtagaatgat gaaaaagttt ttttcgaatt
    22741 ttcctcgcat tttatgaaaa ttttctgtag aatcacatca agagttcaat aaggctaacc
    22801 taataatgaa aaacatgtgt tgaaacgttt gattattttt tcttgttgac tcaacaacga
    22861 aaggagtttt tgtatggaag aagaaaagtt aactcgaagt cttagtgccc gtcatatcca
    22921 aatgatcgca ctaggtggaa cgatcggtgt cggtctgttt atgggtgcct cctcaaccat
    22981 acgttggaca gggccctcag tgatgggagc ctatgcgatt gcaggattct ttttgtattt
    23041 gattatgcgt gctttaggcg aaatgcttta cgttgatcct tcgactggtt cttttgcaaa
    23101 ttacgcgagc gagtacatcc atcctgtggc tggctacttg accgcttgga gtaatatttt
    23161 tcaatatatt gtcgtaggaa taagtgaagt gattgctgta ggatcttata tgaattattg
    23221 gtggcctgat ttgccggcaa ttattcccgg aatcattgtc gtcttgtttc ttatgctggc
    23281 aaatcttatt tctgtcaagg cgtttggcga attagaattt tggttttcga tcatcaaagt
    23341 cataacgatc attttgatga tcattgcagg tcttggggta atcttatttg gatttggtaa
    23401 tcatggtcag gcagtaggga tttcaaatct ttggaaaaat ggtggattct tcactggagg
    23461 cgtcaaagga tttttctttg cattatcgat cgttgttgct tcctatcaag gaatcgaact
    23521 gatcggaatg actgctggtg aagcagagaa tcctaaaaag acgatcattg aagctgtcca
    23581 gtccactatc ggtcgaatct tgatttttta tattggtgct atttttatca ttgtgagtat
    23641 ctatccgtgg aatcaattga gtgagattgg ttcacctttt gtacaaactt tttctaaagt
    23701 cggcattaca tttgctgcag gactgattaa ttttgtcgtg attacagcgg cattatcagg
    23761 ttgcaattca gggattttca gcgcaagtcg tatggtctat acacttgcca taaatggaaa
    23821 gatgtctaaa aaatttctga aactatctcg tcacggtgcg cctttttatc ctgtcgtagc
    23881 aatctcaggt ggaatcttaa ttggtctgat cttaaattat cttctaccaa tgttcatcaa
    23941 ccagtcagaa aatattttcg ttttcgtata cagctcaagt attttgcctg gtatggttcc
    24001 ttgggtagtg attttgatca gcgagattcg cttcaaaaag aagcacgcag ataaaatgca
    24061 tgatcatcct tttaaaatgc cactatctcc ttatacgaat tatgtgacac ttgctttttt
    24121 agctatcgta ttgattttca tgtttatcaa tcctgaaacg agaatctcat tgattattgg
    24181 actcgttttc ttgctttaca tgacgatcca ttactttgta cgcgaaaaaa gaaccggaaa
    24241 ataaaaaaag aacgaagctt caccttagtg gaagcttcgt tcttttttat tgcttttatc
    24301 aattcaattt tagcgtctca ccgggagtca ggaaataatt ctccatgctc atcccattca
    24361 gcgacaagat cgtttctact ggtacgccag ttcgttcggc tatttgattt ggaccttctc
    24421 cttccaaaac tacggttgtc gcagcctgtg aagtcgcgct gttggtatta gtttcagagg
    24481 aatctgttgt gtcttgtgtt gttcgagctt gcatttcaga agcttctacc gactctgctg
    24541 tctcagaatc agaagatgtt gtgtcttgtg tgcttgttga ctctgcttga agagaggcgc
    24601 tagtctgcaa ataaatatcg gaaatcttag cgacatgatt cgtataatta gccgataatt
    24661 gattgatcaa agtatctaag tctacaccgt ctttattttc tgctgcctga tacgctgcac
    24721 gtaattttct gatcccttcg tcggagattg cttgaagctc acgtgctttt tggttagcaa
    24781 ctgctgataa accagctatt ccctcttgat ttccctgaat ttctgtctgg tattcttcaa
    24841 tcaatgcagg cgtcttttct tcgatctttt tcgaataatc atttacgatt gcttgaagtt
    24901 gctctggcaa accatttgct gttttttctt cagaagtttg tgtctctttt gttttagttg
    24961 atgactcact tgtttgtttc gtctctgttt tctttgtcgt tgtttccgtt atggtcgtac
    25021 ttttcgcttc tgacgaattt tttccatgaa aatatagacc cgcaactcct cctgctatga
    25081 tcaaaaacac ggcaactgct gccccaatca tgatcatccg cttacgagct gcctgttttt
    25141 gctgtcttct tcgggtcatc cgaacatttt ctccccactc attttcatca tacatttttt
    25201 tcaccaccaa tttttatgta taatagcgtt atcaaacgct tcatattctt attgtagcaa
    25261 ataattgtcg ctgtgcacat ttttatcttt gttatttata agaaaatttc accagttgag
    25321 atgattccaa gattgtccgt cgatttctcc ttgcagctgc attgttcgtt cgtctatctc
    25381 agccatcagt ttctttattt catataagag cgcacggtct tgatattctg atttttcgct
    25441 taattcatct aattgttgga tcagccaatc atttgtcata atgaagttcc tttcatttct
    25501 tgcagtgtaa aggtcaagtc ttcgatctgc tcttttgcct cagtcagtgc ttctacgata
    25561 tatgcttgtt tttcagattc ttcttcctgc attttctgat tttcctgtac tctttgaata
    25621 aaccagtcaa tccgctgagt cagcgatggt ttgccttcca atgagagata ctgctgataa
    25681 gcaattagct tctcccaacg tttttcctgg gataaataca taaattgtgc taaaacgaat
    25741 ggcggaagat aggtcatatg gaatttattt gcgattcgct gatacggcct caaaaattcg
    25801 gaaatcgtat attcttcttt tcctccagct tggtattctt tttccgccac acctgttgtc
    25861 aatacgattc ctagttcttt cccttttaat acatgtgctg ctcgttccaa gacattatct
    25921 tgccaaattt ttaaatgagc aggagaacta taccagtaaa acggaaactg gaaaatgatc
    25981 cgatcatgct gggccaagag ctgtagttca gtctctatat ctatttttcc gtctggataa
    26041 cttgattcca aatgatgcca agtagctggt aaaccagcag aagcttcttt gaaaaattgc
    26101 tgatttaaag attcttcgat cgttggatgg ctaatgatga taagtgtacg catccctttt
    26161 cctccttttt agtcttcgct ttacagtgta gcataaccgc cacaagtttc ttttgccgat
    26221 gcttgtcatt ttaaccagac taatcaggtt gtccaagaaa ctgcggtttg tttttccaac
    26281 tgttttttca aggtatccaa agcaatattc ttaaaatttt cgtttttcat acgtttacga
    26341 tagttcgtag ccgtttctcc ggtactcttt ttgaacattc ggctaaatac gactagacta
    26401 ttgaacccta cttgataggc aatactttcc acggattcac ccgtcagaga aagaagaaac
    26461 gcccctcgat tgatccgtac cccgatcaaa tattccttga tactggtacc catatgattt
    26521 ttgaatactc gcgacaaatg gctccgattg accgctaaat gctgggcgat ttcctcaaca
    26581 gacaattttt cagcataatg tgcctcgatg tatttgactg tttcaacagc taaagaggaa
    26641 tgaacctttt gtacaccacc gctgaccttt ccgccatctt ttaaaaggac tgataaaaaa
    26701 cggtaggtta atgctgttaa ctgtaattca ttttccaagg tttcttccgt atctttcatg
    26761 cattctaaaa taatgtcgat atatttttgt atgtcagaag agaccatcgt gtattggtct
    26821 tctctgaaaa gcgactgccg aatgatttct tctgcagcac tcccaccaaa tgaaagccag
    26881 caatacatcc acggatcttc tccatctgat aagtaaaacg tcgagtctcc tggacgaatc
    26941 aagaacagat cacctttttt caattggaat tgttgatttt tcacatgata agatcctttg
    27001 ccttccatca cgacatgcag gatgtaatca ttgcgaatag caggaccaaa agaatgaaac
    27061 ggcatcgttt tagagaaacc acaaaaatca aaatacaatt ctttttctac ttgttgatgt
    27121 tcctttttta aaaaaatcga ttgctccacg tctatccctc cttactcctt tagtataatg
    27181 caacaaaaag ttaaataaaa gtcacagaaa tatattaaaa aggaagcgat ttcttgttaa
    27241 gtttaagtca ttaaaagatc tttgttattc gttgtaaccg gtttacttag tattatatat
    27301 tgaacaggag aggaatacag attatgaaaa gcaaaatgat ccaacgtgtg tcctacgctt
    27361 taggcgcttt tggacacgac acattctatg ttacattaag cacttatttt atggtattcg
    27421 tgacaagtca gatgttcgca ggaacagata aggcaacgaa tgcaaaaatg attgctacag
    27481 ttacaagctt agttgtaggt atccgcttag ttgaaattat ttttgatcct ttgattggtg
    27541 gcatcatcga taatactcgt acaaaatacg gtaaattcaa accatggtta gttatcggcg
    27601 gcgttgttag ttccgtactc ttggtagcaa tctttaccaa tttctttggc ttagctactc
    27661 ataatacgac tttatttatc atattatttg caatcgtttt catcttattg gatagttttt
    27721 attcatttaa ggatatcgct ttttggtcaa tgattccaac tttgagtacg gaaacaaagg
    27781 aacgtgaaaa atttgctacg tttgctcgtt tcggctcttc tctaggtgca aatggtacta
    27841 ctctagtcgt tgtccctatc gttactttct ttacctatct tgttactcat aaacatattg
    27901 aaggcgcttc aggctggttc tggttcggtg tgattgctgc tgttatttca ggcggtactg
    27961 cgattatcac tgccttgggc acaaaagaag tcgatactgc gatccgtcag caaaatgaaa
    28021 aagctgggat caaagatgtg ttcaaagcag ttgctcaaaa tgatcagttg atgtggcttg
    28081 cccttgctta tatcgcttat gcgattgcca atgtagcaac aactgccgta ttattctact
    28141 tcttcaaata tgtacttggc caaccaactg aattttggat cgttggtcta gttgctgcct
    28201 tacttggttt agtagctgtt ccactattcc caatgcttac acgcgtcatc tctcgtcgct
    28261 acgtttacct tggagggatc gcaatgatga tttcttctta ctttttcttt acgatcgcag
    28321 gttcaagttt gccaatcgtt atcatcgggc tagtattgtt ctacttccct caacaattga
    28381 ttttcttatc cgctttgatg acgatcacag actctgttga atatggtcaa tggaaaaatg
    28441 gtatccgtaa cgaagctgtt actctatcaa ttcgcccttt actagataaa atcgctggtg
    28501 ctttgtctaa cgggatcgta gggtttgtcg ctagcgcttc tgggatgact ggtaatgcta
    28561 ctgctgctga tatcactgct catggcgtaa cgactttcaa agcttatgca ttctatgctc
    28621 ctgctgcttt gatgattttc tccgctatta tttttgcttg gaagattaca ttgacagaga
    28681 aaaaacatgc acaaatcgtt gagaaattgg aaaacaaatt ggctccagct gatacaatgg
    28741 aagatgaact tcacgatgca gtagttcctt cccattaaga aaggatcttg aaaatgaaga
    28801 ttacagaaaa agattttggc caaggttatc acttgataac cttggagaac aaaaacaagc
    28861 tagcactttc tatctctgat ttaggtgcta gaatcgtcag tttaaaatca aatgatcgag
    28921 aattagtttt gggatttgac accgctgaag aatatatcga aaaagatccg tatatcggtg
    28981 cttctatcgg tcgcaccgca gggagaatcg aaaatggcca cttttctctc aatggaaaaa
    29041 cttaccaatt agccacagat ccaaaaactg gacataatct gcatggtggt gctcctggat
    29101 ttgaattaaa aaaatggtct tatgtcattt tgaatggcga aaatgaagct tctgtcattt
    29161 ttactactac aagtcccgac ggagaacatg gttttccagg tacaatggat gtggaaatcc
    29221 gctatacttt gacaaaagat aatatctggc gagtgacctc tcgcggcacc agtgaccaag
    29281 atactttatt caatccgaca aatcatgttt acttcaactt gacgggagat gcttctcagt
    29341 caatcgatca acatgagctt tggctcaata gcgaagctta tgctcctttg cgaacagata
    29401 gtattcctat aggggtaaaa gaaaatgctg caggaagtgc tttcgatttt caaataccta
    29461 aaaaattagc gtccgttttt gctagtgatt tggatcaaaa aaatcttgtt gacggaatcg
    29521 atcatccatt tttcttaaaa gaaaccggct tgggtaaaga agccgctcgc ttgactagcc
    29581 ccgataaaag aatccaagta gatattgcta cagatgcttc tagtgtggtt attttcactg
    29641 caaatttcgg cacagaaaca ccagaaatgc gcaaccgaaa actggcacat cacggtggca
    29701 tcacatttga gactcaaaca gctccaggtg cagagcgctt ctcagcattt ggttcgattc
    29761 atttaaaagc cggatcagta tttgaaacag tgacagaatt caaaatcaaa acaagaaagg
    29821 aataactcat catggaaatc aaaagaagct taaacacaaa atttgccgaa acattcggac
    29881 ctaaaaaaac tacacaatac ttctcaccag gacgcatcaa tttgattggt gaacacacag
    29941 attacaatgg aggccatgtc ttccccgctt ccatcactta tggaacgcaa ggtgtagcag
    30001 cacctcgaga cgataataaa atattagttt attccacaaa ctttgacgat atcggtgtca
    30061 ttagttttac tttagatgag ctagaatatg ataaaaaagc aaactgggca aattacgtaa
    30121 aaggaatggt ccttaaatta aaagaagctg gacactcaat cgatcatggg tttgagttat
    30181 tagtggaagg aacaatccct aatggtgcag gactttccag tagcgcttca ttagaattat
    30241 tagtaggtgt tgtattagaa gatctgtttg atttaaaaac agatcgcttg gaactagtcc
    30301 aaacaggtaa aaaagtagaa aatgaattca tcggtgtcaa ttcaggtatc atggatcagt
    30361 ttgccatcgg cttcggtgag ctagatcatg caatcttatt agatacaaat acgctgaaat
    30421 acgaaatggt tccagtgaaa ctagatggtt atgcaatcgt catcatgaat acgaataagc
    30481 ggcgggaatt ggcagattca aaatacaatg aacgccgtag cgaatgcgaa gaagccttgg
    30541 cacgtttgca aaaaactttg cctatctctt ctttaggaga cttagatgaa gaagaatttg
    30601 aatcaactat tgaccaaatc ggcgatgata cattgatacg cagagctcgc catgccgttt
    30661 ctgaaaatca gcggactttg aaggcaaaag ctgaattaga agctgggaac ttagaagcat
    30721 ttggtcaact gttaaatgat tctcaccact ctcttcgcta tgattatgaa gtaactggga
    30781 tcgaattaga tacactagta gatgctgctc aaaaacaaga aggtgtctta ggtgcacgaa
    30841 tgacaggagc tggatttggt ggctgtgcga ttgctcttgt caaagaagaa aatatacctg
    30901 ccttcaaaaa caatgtctat gatgaataca tgaaagttgt cggctatgct ccggaatttt
    30961 atgttgcaca cattggaaat gggacaacaa agatcgatcc agaagctgtt catgtttgat
    31021 aaaaaaatca acgatctcag ctaaaaacaa atagataaaa ataacctctc agcaatttcc
    31081 aaatgtgagc cttttgctcg tatttgaaaa ttgctgtttt ttattgacac ggaaagagaa
    31141 cgtgtgttct aattgtgttg aagaggtgat tgttatgtgg gcgaatcaaa atccggtatt
    31201 tgtttatgaa aaagaacctt caagagatat tttatgtatt gactgcaaat ctttttacgc
    31261 ctccgtagag tgcgttgaaa aggatcttga tccccttaca actaaattag tagtcatgtc
    31321 ctatccgtcc gattcgacag aaacacgggg tagcggatta atcttggctt ccagtcctgc
    31381 agcaaaaaaa gcttacggca tcaccaatat cagccgtgcc agagatcttc ccttccccta
    31441 tccaagtgat ttatatattg ttgccccaag gatggcctat tatatggaaa agaacagaga
    31501 aatcaattct atctataaaa aatatgcaga cgaagcgaac catcatgtct attccgtcga
    31561 tgaaagcttc cttgatgtaa caaatagcat ggcactcttt ggtaaaacaa atgcgtacga
    31621 catggctaaa ctgatccata aagatgtgtt tgagcagacc ggaatctaca cgactatagg
    31681 aattggcgat aacccgctac ttgctaaact agccttagac aatggctcaa aagacggaaa
    31741 gcaaatgttt gctgaatggc gttatgaaga tgtaccagaa aagttatgga aaatctcaga
    31801 tatcactgac ttttggggaa tcggcaaacg aacagcaaaa cgtctgaaac aaatgggtat
    31861 acattctgtt tatgatttag ctcacgcgga ttattatcac ttgaaaagca agctaggggt
    31921 catcggtact cagctttatg cccatgcttg gggcatcgat cgaagttttt tgggacaaga
    31981 atatacacca aaatcaaaaa gtatcggaaa tagtcaaatc ctagatagag attatacaag
    32041 acgagaagaa atcgagatcg ttatcaaaga aatggccgac caagtcggca ctcgcttgag
    32101 acgatctgca gcaaaaacac aagtagtcag cttatgggta ggatactctt taggctatct
    32161 tgatccaaat ggcaagaccg ggtttcacca gcaaatgaaa atcgaagcga caaatgctag
    32221 cagagatcta gcagatgctt tattgaccat tttccaccag cattaccact accaggatat
    32281 tcggcgtgta ggcgtgaact gttccgacct cgtatacgcg acaggtttac agcttaacct
    32341 ttttgaagat cctgaaaagc agataaatga agcgaaagtt gattttgttg tcgatacgat
    32401 tagaaaaaaa tacggctttc aatcaatcat ccatgcgcat agtctcttgc aaggaggacg
    32461 cgccatcgca cgtagttctc tcgttggcgg gcatgcaggt ggtatgagtg gattagaagg
    32521 tggaaaagat gagacggaca gcaaaaaaat tcattgacta taacgagtat cgagatcggc
    32581 catttggatt aaaatggggg actgcttttg cgatggaaga actagtcaac ggtatcaaaa
    32641 aaaatgaaga agaagcacgc aaacagcctc ttcctaaaca acaaatgaca ttgcaagaaa
    32701 tcgatagtat actgacagaa gcctttttat ctcgtaaagc agtaatcatt cagctcaatt
    32761 taaaagatga atttggaaga ctcttggatg aggtcgaagg gcacttcatt ggtgaagcct
    32821 atgaagatta ctttatgatt gaccatcagc cgatattgtg ggaagacgta cggtatattg
    32881 agttaaagga aacaaaaaaa tggtttgtga catgagaggt tgtgacagaa gtgatcaact
    32941 tcaaaaaata agaaaggatt tttcgaaaat tgcttcttaa atttttgtgg aattttggct
    33001 tatttttcga agaagcaact tctggaacaa cgtatattag atatcagagg ttgtgaaaaa
    33061 gtcgcttagt tttgagtaac aagccaaggc gctgatttca caacctctgt ctatctatcc
    33121 atctattcgg taagattcat gcatgacgtc ttctttattt agtatgtaga gccctttttc
    33181 ttctgaccat tcaacataaa tcagctgttt tacatctgca tagccttctt tcttcaagtt
    33241 ctccataagc catgcttcag tcaattgatt ggactgaagc actttatgct gaatttgccc
    33301 ttcatctaca agcaggatcg ataaagcttt gtcttcatct gattttttca atacactcat
    33361 ttgaccggca ttctctagga taacatgttt ggcattttca agaaaaaagc attcttgctg
    33421 tcttaagatt gctcgcaact gctccatctc gatatggtta tttgtcaact ctgtcatgtt
    33481 gatgacgccg tctttgatta gaacagatgg ctcgcctttg acccaacgat tgaccttttc
    33541 atttttttgg acgatacgtt cgataccata aatcattact gcccaaagcg caatggcaaa
    33601 taggacatgc cctacatgga cattgtcatc ataggttgat tcttctaaga tcccacctaa
    33661 aacaagcgta tagactaaat caaacggtgt gatttctgac atggatttct tcccaagcaa
    33721 acgtaccact aataataaac cgatcaaccc gaacaccagt ttcattgtaa ttgaaaaaat
    33781 cattttttct ccccctaact atctgttaat taaattatat atgctttctc acgaacacgc
    33841 atggaatata caaggaagaa atgtatcctt ttactaaaaa aaggactgtg aca
//
LOCUS       DAZKCY010000019        33323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000019 DAZKCY010000000
VERSION     DAZKCY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.019"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..200)
                     /locus_tag="ACG8XC_000809"
     CDS             complement(<1..200)
                     /locus_tag="ACG8XC_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFP9190526.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWE"
     gene            complement(197..715)
                     /gene="msrA"
                     /locus_tag="ACG8XC_000810"
     CDS             complement(197..715)
                     /gene="msrA"
                     /locus_tag="ACG8XC_000810"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9190527.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(735..1448)
                     /locus_tag="ACG8XC_000811"
     CDS             complement(735..1448)
                     /locus_tag="ACG8XC_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFP9190528.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(1451..2347)
                     /locus_tag="ACG8XC_000812"
     CDS             complement(1451..2347)
                     /locus_tag="ACG8XC_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9190529.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(2505..3347)
                     /locus_tag="ACG8XC_000813"
     CDS             complement(2505..3347)
                     /locus_tag="ACG8XC_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9190530.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3479..4132
                     /locus_tag="ACG8XC_000814"
     CDS             3479..4132
                     /locus_tag="ACG8XC_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP9190531.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(4195..4722)
                     /locus_tag="ACG8XC_000815"
     CDS             complement(4195..4722)
                     /locus_tag="ACG8XC_000815"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP9190532.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4742..5689)
                     /locus_tag="ACG8XC_000816"
     CDS             complement(4742..5689)
                     /locus_tag="ACG8XC_000816"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP9190533.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(5701..7584)
                     /locus_tag="ACG8XC_000817"
     CDS             complement(5701..7584)
                     /locus_tag="ACG8XC_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9190534.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(7747..9825)
                     /locus_tag="ACG8XC_000818"
     CDS             complement(7747..9825)
                     /locus_tag="ACG8XC_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP9190535.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(9838..10113)
                     /locus_tag="ACG8XC_000819"
     CDS             complement(9838..10113)
                     /locus_tag="ACG8XC_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190536.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(10475..10930)
                     /locus_tag="ACG8XC_000820"
     CDS             complement(10475..10930)
                     /locus_tag="ACG8XC_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190537.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(11084..12292)
                     /locus_tag="ACG8XC_000821"
     CDS             complement(11084..12292)
                     /locus_tag="ACG8XC_000821"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFP9190538.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(12404..12736)
                     /locus_tag="ACG8XC_000822"
     CDS             complement(12404..12736)
                     /locus_tag="ACG8XC_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP9190539.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            12884..13759
                     /locus_tag="ACG8XC_000823"
     CDS             12884..13759
                     /locus_tag="ACG8XC_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9190540.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(13841..14422)
                     /locus_tag="ACG8XC_000824"
     CDS             complement(13841..14422)
                     /locus_tag="ACG8XC_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFP9190541.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(14428..15687)
                     /locus_tag="ACG8XC_000825"
     CDS             complement(14428..15687)
                     /locus_tag="ACG8XC_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9190542.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(15689..16735)
                     /locus_tag="ACG8XC_000826"
     CDS             complement(15689..16735)
                     /locus_tag="ACG8XC_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190543.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(16970..17245)
                     /locus_tag="ACG8XC_000827"
     CDS             complement(16970..17245)
                     /locus_tag="ACG8XC_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFP9190544.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(17488..18798)
                     /gene="der"
                     /locus_tag="ACG8XC_000828"
     CDS             complement(17488..18798)
                     /gene="der"
                     /locus_tag="ACG8XC_000828"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP9190545.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(18980..20209)
                     /gene="rpsA"
                     /locus_tag="ACG8XC_000829"
     CDS             complement(18980..20209)
                     /gene="rpsA"
                     /locus_tag="ACG8XC_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP9190546.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(20344..21024)
                     /gene="cmk"
                     /locus_tag="ACG8XC_000830"
     CDS             complement(20344..21024)
                     /gene="cmk"
                     /locus_tag="ACG8XC_000830"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP9190547.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(21073..21708)
                     /locus_tag="ACG8XC_000831"
     CDS             complement(21073..21708)
                     /locus_tag="ACG8XC_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFP9190548.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(21774..23177)
                     /locus_tag="ACG8XC_000832"
     CDS             complement(21774..23177)
                     /locus_tag="ACG8XC_000832"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP9190549.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(23164..24195)
                     /locus_tag="ACG8XC_000833"
     CDS             complement(23164..24195)
                     /locus_tag="ACG8XC_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9190550.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            24226..24447
                     /locus_tag="ACG8XC_000834"
     CDS             24226..24447
                     /locus_tag="ACG8XC_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFP9190551.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(24612..25349)
                     /locus_tag="ACG8XC_000835"
     CDS             complement(24612..25349)
                     /locus_tag="ACG8XC_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFP9190552.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(25502..26842)
                     /locus_tag="ACG8XC_000836"
     CDS             complement(25502..26842)
                     /locus_tag="ACG8XC_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9190553.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(27079..28602)
                     /locus_tag="ACG8XC_000837"
     CDS             complement(27079..28602)
                     /locus_tag="ACG8XC_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFP9190554.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(28981..29592)
                     /locus_tag="ACG8XC_000838"
     CDS             complement(28981..29592)
                     /locus_tag="ACG8XC_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9190555.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(29715..29840)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(29922..30638)
                     /locus_tag="ACG8XC_000839"
     CDS             complement(29922..30638)
                     /locus_tag="ACG8XC_000839"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP9190556.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(30644..31213)
                     /gene="scpB"
                     /locus_tag="ACG8XC_000840"
     CDS             complement(30644..31213)
                     /gene="scpB"
                     /locus_tag="ACG8XC_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFP9190557.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(31197..31967)
                     /locus_tag="ACG8XC_000841"
     CDS             complement(31197..31967)
                     /locus_tag="ACG8XC_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFP9190558.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(31964..32335)
                     /locus_tag="ACG8XC_000842"
     CDS             complement(31964..32335)
                     /locus_tag="ACG8XC_000842"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9190559.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(32354..33241)
                     /gene="xerD"
                     /locus_tag="ACG8XC_000843"
     CDS             complement(32354..33241)
                     /gene="xerD"
                     /locus_tag="ACG8XC_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP9190560.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         9294 a         7053 c         5661 g        11315 t
ORIGIN      
        1 ttttcccatg cttcgtcaaa gatatccatg gacggcaaat agtcgacatt cagttccaga
       61 taattagaaa tttcttcgta gctagagctg tgcttaggaa attggatgtc tttaccggct
      121 tctgtagcga aaacagataa agcatcatgt gtgccaccgc ctcgcagtgt cattaaataa
      181 tgaaaaaagc ttcgattcac ccttcgttcc tccaattttc ttcgataaag gcagcccggc
      241 gtgcatggtt tcttttaaaa ttttcaggat gctttttata atactcttga tggtcttctt
      301 ctgctagata aaatggttgg gctggttcga tttttgtaac gattggctgt tcgaaacgtc
      361 cactttcagc taactgtttc ttactttttt cagcaatgat ccgctgttct tctgtcaaat
      421 aaaagatcac cgggcgataa ttgtcaccgc gatcctcgaa ttgtccgaaa gcgtcagttg
      481 gatcagtttg ctgccagtaa agttctacta attgttcata tgaaatcaat gttggatcat
      541 agataatctc cactgcttct gtatgtccag tcgtttggct ttttacttgt tcataagtcg
      601 gatctggtac atgccctcca gtatatcctg acatcactgt atggatacca ggaagcgtgt
      661 cgaaaggctg gatcatacac cagaagcagc ctcctgcaaa tattgctcgt tccattatga
      721 ttccccctct aatactactc atccgatgat tttttatttt cttttgtctt gctttcagta
      781 ctgctggtgc tagttttgtt ggtatttgag ttgtttgaag catcagaggt atcttttggc
      841 aaatatagac taaaccgaat gtcatcattt actaagtcga ttttatctgc tttaatatac
      901 atcccgtttt gcattcggaa ttgatccaac ctaagcatga tagtcagatc atctgtattg
      961 acttcgatcc aatctggtaa tttatagttt ctcttgatca tattcatcac atctttgatt
     1021 ggcaaactca atgttccgat agaaagactt ctagctttta gttggacatt tccgttttcc
     1081 atcacatacg gatcaaaata taaatagaaa tcgataggaa aacctagtac ttcgaaagta
     1141 ccattgagta gtgcttcatt ttccaaataa aatttatatt caatatcact atctttttga
     1201 tactcactaa ggaaaaagct gatgatttga ttgacttttt ctttatttga attgatcgtt
     1261 aagacaggat ctccttgtct ttccgtaaga gctggtattt ctgggagttc tggttcccga
     1321 ttagcaaaaa tacggctgcc taaaaatacg gtcgaaccaa gtactacccc aagaaggata
     1381 aggaaggcga atttccagta atttgttttc tttgttttgt ttgaagatac gtcagatctt
     1441 gttttcatgt ttattgatca tcctccttct ctaaccattg gttttgtgtc tcgatcagct
     1501 gatctctcac tgcatttgcc atcaattgat aacctaaatt atttggatgg aaatgatctt
     1561 cttcatagag cacattattt ttgatgccat ttagattttc tgtcgtgcta gttgtttgat
     1621 ctgttccttc ttctgtgaca cctacttcct gattcaatcc ttgataaagc aaatcattga
     1681 ttgggataaa gtagcagttc ggcatttttt ttgtcaccgt ttctgttcct ttgttccaat
     1741 tgttaacaat tgtctgcatg tcagtgatct caggaaagtt caaataaaat ggattgtaga
     1801 tgcctaaaat gtaaataggt gcggttttat tgagggatcg aaattcttcc aatagttctg
     1861 tgacattttc ctgatagttt ttcagtggct ttttaaagga attgaccgtt aacccgaata
     1921 aattattttg gataactttc atcaaatcat taccgccaac agtcagtgtg ataaaatcag
     1981 ctgttttgat attgtttcga atttcttctt ttgtttttac tcgctttaag atctggtcac
     2041 ttcgttcacc atttacacca tagttttctg tttcaaccgc ggtcaaatta taacggtctt
     2101 ttagatcatt tgcgacaagg ggaacgaatc ctccttgctt ggtttgatct cctactcctt
     2161 cagtcagcga atctccgata gctatgtagt gaagtttttc ttttttattt ggatcttctc
     2221 tcacagtagt tactggtttc attagtgggc cagctttcgg tatagaagca gctaaacctg
     2281 catagatgat gatcgcaatt aataatggga acaaaacgag gatcaaatgt cgtttcgtta
     2341 atttcatttt ttcacctctt gtattcgatt ggttctaatg ttatcaaaaa ttttatcaaa
     2401 aaggtagtct ttatgaaaaa agaaaacaat tattttccta attttataaa gaaaaagaga
     2461 ataagctgca agcctttcag gctgagggct tatcctcttt tcgactaatc tacataatac
     2521 atgattgcaa acgcgtcttt accagtatgc gtagcgataa ccggattcgt atgcagtaca
     2581 gggatatgca tgtccgggaa taattcttgc aaaccctctt tgaattcttg cgccaattct
     2641 tttgcatccg catgtgaaat accgatttgt cgcacatttg gcaattcttt cagttcattt
     2701 ttcaaattat taaaccattt atcgaacgtt ttctttcctc gccccttagt tacagggatt
     2761 aattcagaat gggcgaaatt catcaccacg cgtatgttca aaacactaga cagcaaacca
     2821 gttgttcggc tgattcttcc accttttacc aagttatcta gtgtggatat tccgatgtat
     2881 aattttgtat tgtcttgtac ttttttgact tcttcaagga ttttctctac tgttttacct
     2941 tcttttgcta attcagctgc tcgaattacc tgaaaagata atccttggtc agtggtatca
     3001 ctgtcgataa ccgttacttt cgtttttgtt agattactgg cttgtcttgc tgcttctact
     3061 gttccactca agccttttgt catatggatc gaaatcactt cactgccgtc ttctcctagc
     3121 tcatcataca tcgagatgaa ttctccaatt ggtggttggc ttgttttggg cagatttttt
     3181 gctttcgcca tcatttccat aaatctttcg ccgtctagtt ggtcatcatc gggatatact
     3241 gttccgtcga tcatgatgga tagcggcatg acacgaatgc ctaaagcatc gcgtacattt
     3301 ttttccatcg tacatgaaga atctgttacg atttttacat ttgtcattat tttctctctc
     3361 tctttcttta acaagatgcc ttattcatgt taaactacat gtaagacaat ttctattttt
     3421 agtataacag acaaataatg atttttcatt aattacacct agtaaaggag ccgttttcat
     3481 ggaaaaaacg aaatttaatc gaacgtatga aatattaaat gaagtgttta atgcagtcac
     3541 ccatggaatt gggactggat tgagtatcgc gggactagtg gttcttttag taaaaggtgc
     3601 ccacttgcat tcctccctcc atattgtctc ttatgctatc tatggatcga tgatgattct
     3661 tttattttta acgtctactt tgtttcattc attgattttt acaaaagcaa aaaaggtgtt
     3721 ccaagtattc gatcatagtt cgatttttct tttgattgca ggaagttata ctcctttttg
     3781 tttgctgagt atccgaggat ggctaggttg gactttattt gtattaatct ggttgtttgc
     3841 gattcttggc attatatata aagcgttgac gttgcataaa cgggagagag tatcaaaagt
     3901 atctacgatc atctatattt ttatggggtg gctctgtgtc gtggctgctg ttcctcttta
     3961 tcactctctt ggaatgactg gtgttatttt gatggtagca ggcgggcttt cttacacgat
     4021 aggcgcattc ttttatagct tgaattctat ccgctatatg catgttgttt ggcatttgtt
     4081 tgttatgtta ggtgcaggtt ttatgtactt ctctgtttta ttctttactt aataaaaaaa
     4141 ataaagctat ggatgggaca gctctaaata acagctagtc tatccatagc ttttttatat
     4201 cgtcaattcg cctgtcccgc tttttacagg attctcatta cgctgatacg tttcaaattg
     4261 atgcggataa cgattttttt catccatttc tccttgacta gtattgatca gtgaccaatc
     4321 agaccagtca acaggtggaa aataagcatc tccatcgaat acatgatgga tgactgtacg
     4381 gtataatatc gtacaaaatg gcagaaattc ttgataaaca gctgaccctc cagcgatgaa
     4441 aaagataccg tctgtttctt tttccagagc aagcacgtct tccacactat gcatgacatg
     4501 cacgccttct gctttatagg aagaatctct cgtcaaaaca atcgtctgac gattcggcaa
     4561 cggacggcta cccatccctt cgaatgtttt tcgtcccatc actagtgtat ttgcttctgt
     4621 catttgtttg aagaatttca gatcatttgg caaacgccaa ggaagacggt cttcttttcc
     4681 aatcaatccg ttttcatcct gtgcccaaat tgcagctaac attttctcat cctttctgat
     4741 tctaaacagc gataggagct ttgatcgccg gatgtgggtc atatccttct aattggatat
     4801 cttccatctc aaaatcaaat actgactgct tttcctgatt caaaactaaa gtcggaaatg
     4861 cgcgtatttc tcggctcaat tgttctttca tttgttcgat atgattgata taaagatgag
     4921 catctcctaa tgtgtgaaca aattcgccaa ccgctaaacc tgtttcatgt gcaattaggt
     4981 gagtcagcaa cgcataactt gcaatgttga acggaacgcc tagaaacaca tccccactgc
     5041 gttgatacaa ttggcagctt agccgtccct cttgaacata aaattgaaac atcgtatgac
     5101 aaggaggcaa agccatgcta ggaacatctt ccggattcca tgcagaaacg atcaatcttc
     5161 tagaatcagg agtctttttg attgcttcga tcacttgttt gatttgatcg ataaagccac
     5221 cgtcttttgt ttcccagtga cgccattgat agccgtagat gtgtccaaga tcgccgtatg
     5281 tttcagcaaa ggaatcatcg gttaggatct tctcgcaaaa aagcttgctc tgctttttat
     5341 attcttcatt gaattcagga tctgtcaaac atctatggcc aaaatcagtc atatctggtc
     5401 cattatagtc tggacttttg atgtagcgtt caaatgccca ttcatcccaa atatggtttt
     5461 tattttgtaa tagatagcgg atattcgtat ctccttttaa aaaccaaagc aattcgcttt
     5521 tgatcaatcc aaaaggtact cgtttcgttg ttaacaaagg aaaccctttt tgcagatcaa
     5581 accgcatttg gtatccgaat aagcttaatg tgcctgtgcc tgttctgtct tctttacgat
     5641 gcccttcttc caacaatttt cttcctaagt caagatacgc ttgttccatc ctttttcctc
     5701 ctaatcttct acccattcac tcaagtattc ccaccgtgcc attttttctt caagttgtgc
     5761 ttctgtttca gatacgtcgt tttgcaattc ttgtaatcga gtgaaattat ctccttgatg
     5821 attcatctct tcttggagca atgagatttt ttcttcaagt tctgcaatct catcttcaat
     5881 cgtttcccat tctttttgtt ccatgtaaga taatttcttc ttttcagcag gttccttgct
     5941 ctgtattttt ggtttcgctt tttcagcagg ctgctgggtc tctttttgct tcagtaaata
     6001 actggacatg tttccgtaat aaagttcttg ttttccgttt ccttggaata ctaataactt
     6061 atctgctacc ttgtccaaga aataacggtc atgggaaaca gtgatcacag cacctggaaa
     6121 acttctaaaa taatcttcca aaatcgtcaa tgttgctatg tccaaatcat tggtcggttc
     6181 atccaataat aaaacattag gttgctgaat cagcagtttc aacaaaaata accggcgttt
     6241 ttctccgcct gaaagtttgc ctatcagtgt cccatgcata aatcttggaa ataaaaatcg
     6301 ttcaagtaat tctgctactc cgatttgact gccatctgct gtttttgctt cttcagcagc
     6361 ttcttgtaag taagcaatca tccgtttatc tggtgccatt tcttcatttt cttgtgtata
     6421 atacgcaaga tgaaccgttt caccgataga cattgtaccg ctatccaggg gaattctgcc
     6481 agctagaata ttaagcaatg tcgattttcc cgcaccattt ttaccagtaa tacctagtct
     6541 ttcccgagat tgaatcaata aatctaaatg ttctagcagt gtttggtcat ttatacggta
     6601 actgccatct ttgatttcca gtactttttt ccctaaacgt tgtgtcgcta ggttcagctc
     6661 taaatcatct tcttggttta cttggtacaa gttctccttt agatcttcga aacggttgat
     6721 ccgtgcttgc tgcttggtgc cacgtgcctg tacgccagcc ctcatccaag ctaattcctg
     6781 tttataaagc cgcttgcgct tttcttcttg ttctacttct acacgatcac gttctgcttt
     6841 ttctaagacg taagcttcat aatttccttt atactcgtat aattttccaa atgaaagttc
     6901 gaagattcgg tttgctactc tatctagaaa ataacgatcg tgggtaacca tcaatagtgc
     6961 gccacggtat tgtttcaaat agttttccaa ccactcgatc gcttcataat ctaaatggtt
     7021 tgttggttcg tctagtaata gtaaatcggg ttcatcaatc aacacttgcg ccagactaac
     7081 tcgttttttc tgaccacctg ataaagtact gattttttta tctaattcac tgattcctaa
     7141 tttttgcaaa ataatctttg cgttggtatc tgtagtccaa gcatcttctt tattcatttg
     7201 ttcttctgct ttagcataac gtttttgcac atcagggtct tctccgtttt cccctagttc
     7261 gatcaatgct tcctcatatg ctcgaacagt ttggatcaag gggctgtttc cttgaaagac
     7321 tgcttggaga acagttaatt catcggaaaa ttcagctgct tgagaaagat aaccaatacg
     7381 gtaatcagtt ggataaaaca ctgtttgccg atcaccgtcg ctgctatcga taccggcaat
     7441 aatatttaag agacttgttt tacccgtccc gttcgtccca atcaacccga tacggtcttt
     7501 ttcgtggacc aaaaaagaaa tctggtcaaa caaatctttt tcaccataag tttttttgag
     7561 gtcagttact tttaattctt tcaacatgtt ccctccttat cttggatatt gtatcataaa
     7621 aatagtgaac ttcaggaaaa tttttttggt atttgttctt tttgtgttct caaaaaaaga
     7681 acaggaccga ttcgatcctg ttctgatatg ttttgttttt tataaaactt gaaaattttt
     7741 acctttttat tttattggac tcgttttttt tgactttgct cgaaacgcaa atcggaaaga
     7801 aaagttatca gatggtttgt tcatcgtata agattctaac aaattgatat tcctgcgata
     7861 gctgttcgca gaaaacgtcg tgattttttc agcaacttcg tattcatcaa tgatttttct
     7921 ttcaagataa taacctctta gcattttttt cgtaaacaga ctatcttgct ggatcatcca
     7981 cgaagaaatc aaattccccg agttcatttt tattccttca agtttgcgtt gctggataaa
     8041 aaattgaacc atcgtttcat cataaatatc agtcaagtta tctaaacttt caataatcaa
     8101 ctgagtattt ttttgatatg tctctctgat ttcttggata tcttcatcac taataaatgt
     8161 ctgacgatca gcatatcttc tttgccaaaa cattttcgga tggagaacga ttcgcattaa
     8221 cctacggaca acaatgaacc aaaagccgat aaaagacaaa atctggcttg tgatagaatg
     8281 ttcgaatcga gaaagaaaac gggagtaaaa acgataacca ttagaagaaa gcttgcccga
     8341 acgataactt tcatccaatc catcccgttc gatcgacaaa atcagtgctt gtatttcttg
     8401 tacttcttgc ttttcatcat cggttaaatc ttccaagtac agatcacgta tccgttcttg
     8461 ataattttct atcactgctt cagtggctaa aaattctttt gtattacgat caatttcctt
     8521 tatttctttt cgcaaaactt caacgacctc ttctaaaatc atcaaagcat tcaaatccgt
     8581 gcttttcgct tcctctgttt ccgtcagcat aggcaagaca atgatcccta tgaccaaagt
     8641 cactaagatg acacccgccg tcagaaacaa aagcaaagac cgctggtaaa acatcatatt
     8701 attgattgaa aatggcaaaa taaaaatcgt agccaaactg actgttccct ttacaccgcc
     8761 aaatgttaag atcaagatct cgttcaactg ttttttgaac tttttactgc catctttgaa
     8821 tacataaaaa agactgatag aaatgaatct gatcacaaaa agcatgacag agattaatac
     8881 gataatcgct aataaaagac cgtttggata taaaccgtct ccccatactg gcgagaacac
     8941 ttgcgtcaat tcgattccta aaaatataaa tactaatgca ttcagtgtaa aagcaatagt
     9001 tgtccatgta ctgtgggaca agctcgacaa ttctgcatca aacacagaga ttttcctaaa
     9061 accagaagct tggagaatac ctgctgccac tgcagcaatg attccagata catcaaatgc
     9121 ctctgccaat acataggcta gaaaaggaag caaaagttcg atcagcagat accctgtgac
     9181 atcttgtgct gatgcttttt cgatcaaatg aattattttt tgtttgaacc agacaagcaa
     9241 aaagccgatc aaagctcccc caatactaga aactactaga ctcatccccg cgtttactgc
     9301 tgaaaaagat ccggtgatca gcgcaccaag agcaaactgg aaggcagtaa caccagatgc
     9361 atcattcaaa agaccttctc cttccaaaat atgcatcgcc tttttaggga tattcactcg
     9421 tccagaaaga gaactgactg ctaccgcatc tgtcggacct aacgctgctc caaaagcaaa
     9481 gcaggcagca agtgggacac ttggcaacag gaatgataac gtcgcaccta ccccgacaag
     9541 ggtcaaaatg actcctccaa atgccaagaa caaaattgtc ccgaaatttt tgagaatcga
     9601 agaaatatct gctttttctc cttctctaaa taacaatggt gcaataatca taactaagaa
     9661 caattcaggt tcaaaatcga ttgattgtcc ccactctgtc agacctaata ccactccaag
     9721 gaaaatttgg atcagtggag ctggaatcat tggcaaaatg cggctagcta tgttcgataa
     9781 ggtgatcgtt actgcaaata caataactaa ctcagcaaat tccatcgtcc atccccttta
     9841 ttttttattt tgtgtaagca tttttaaaaa ttgttctttt ttctttaaaa ttttggtttc
     9901 gactacttca acctgtgttt ggttcccctc taattcacac tgtacacgtt ttgactccaa
     9961 acgaagcaga tcatgtttca gacgttccaa ctgacgatac atcaatatat ttcttttacg
    10021 caacttacaa agtatttgac ggcggtcatc catcgaaagg gcgccatagc gatcatgcaa
    10081 cagttttttt tgttgttcaa aactctctct cattttccag atctcctata cacaaacttc
    10141 catcattata gcaaagttct ttttggatga aaaatatttc attgtttcat cgtttattgc
    10201 tagttagaaa tttccataaa tttttcaacg cctcattcta ttccaaatag tatcagatat
    10261 caagtaaaac ttaagaaaga taaaagtttc tgacaaaagg atgtttactt tcccattcct
    10321 gcctttcttg ttcttttatc acaacaaaaa aatggggctg cgacaaaaat cttgtcacag
    10381 tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat aagacacatc
    10441 tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc ccgtatacat
    10501 cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt tcgctgattt
    10561 ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat gggaagtaca
    10621 caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct tcgtatttca
    10681 atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt gtcgcaactg
    10741 cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt tcaacaaatt
    10801 cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga tcgccgatat
    10861 gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc gctgtcattt
    10921 ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac ctcagtttag
    10981 cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt atctgcccac
    11041 tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc aatttttctt
    11101 tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct acgacggctt
    11161 tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca gtttcttcta
    11221 gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa cgatcatagg
    11281 attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat tcttctactt
    11341 gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct ccagtttctc
    11401 gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta gaacacttcc
    11461 acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg atcaataatg
    11521 cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa cgtaaaagtg
    11581 cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca ataaatgcat
    11641 ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca tttatacgtt
    11701 ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag gtttcttcct
    11761 ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt aaggcatctt
    11821 catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct agatcttgca
    11881 gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca ttgatcgtaa
    11941 aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga tctggtctac
    12001 ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt tcatcatttt
    12061 ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga aaaagctgtt
    12121 tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc tggttcagca
    12181 agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc tctaattttt
    12241 tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc atttttccaa
    12301 cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga ctgtttgaca
    12361 agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg tccttacgct
    12421 caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa acttcagtta
    12481 agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca ccaagttctt
    12541 ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa gtgtggttga
    12601 cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct aatggagaga
    12661 aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata ctgtataaag
    12721 aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt aacgcaaatc
    12781 ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag gaatttctaa
    12841 atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca aacgaaaaac
    12901 agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg gtcttgttta
    12961 cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct tgatcttaag
    13021 agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc cattgatttt
    13081 actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag gtacactttc
    13141 tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt tacagcatga
    13201 tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg ggatcgtcta
    13261 ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag aacaaaatta
    13321 cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac ttgcttcatt
    13381 aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg ttttcagtcg
    13441 tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg tcgtctccaa
    13501 caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg tgactttcct
    13561 ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg tcgttagctc
    13621 tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag ctttcctttc
    13681 aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc cacagacaag
    13741 attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag attccacatc
    13801 cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta tgttcactta
    13861 cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca tcagataatt
    13921 tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca taaagcattt
    13981 ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca gtatcttctt
    14041 cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat agatattctc
    14101 ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc caattcaaac
    14161 ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa aacaatttca
    14221 tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca aaattcgccc
    14281 cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa ttcaagatcc
    14341 aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa aaattcttct
    14401 tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta ataatgacag
    14461 tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt gcgctctttt
    14521 cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg gttcatgtga
    14581 tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt ctaattcatt
    14641 ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca tctgattgat
    14701 cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta aagtaagtaa
    14761 tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt ctgctcgctg
    14821 tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga cttggtaagg
    14881 attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt cttcttcttg
    14941 aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt ctcgaagttc
    15001 ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa aagctagctg
    15061 gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta cgatagctgc
    15121 ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg agattcctga
    15181 aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct ctttgaaacg
    15241 atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat caggaagcaa
    15301 cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa cttggtacaa
    15361 atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca ctttttccag
    15421 gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta acgggatgtt
    15481 cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt ttgcttcgat
    15541 caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg ctgggtcttc
    15601 ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg ataaagcttg
    15661 caacattttt tcgctatttg tattcatttc aattccctct tatctcactg acgatttgtt
    15721 gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat ccgtctatta
    15781 tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa agaacttttt
    15841 ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca agcaagtcca
    15901 gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga ttccacacga
    15961 aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg ccattttcgg
    16021 tcagataaaa agcttgttca aacagtttac cataatttga caggttttct gcctctaatt
    16081 gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt
    16141 tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc attccgcaac
    16201 agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg attgtttgat
    16261 cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt ggtagctgca
    16321 aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca gggatcatcc
    16381 atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc atgatccctt
    16441 catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg acgaaatcaa
    16501 gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata taccagtcat
    16561 tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca taatagcgct
    16621 gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga ctgactagcg
    16681 tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt tccatacgca
    16741 tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa agataagagc
    16801 aaagctcacc tcataacttg caaataaata aaaattaaat aaaatacatg tgttttctat
    16861 gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc taggaaataa
    16921 aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct tatttaacag
    16981 cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga atttcgattt
    17041 cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact tcaaagttac
    17101 caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata gttgaaaata
    17161 cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa gcaacttttt
    17221 cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga gatatgatag
    17281 aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa atattgatta
    17341 acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg tttttaccaa
    17401 ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt tttcttttga
    17461 aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa ttcggatgga
    17521 cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt cataagaaaa
    17581 atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga cagcgacttg
    17641 tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat tgattgctac
    17701 agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct ggttcatact
    17761 tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg cagaaacaaa
    17821 aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt cgaaatcacg
    17881 cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa taattccacg
    17941 accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc cttcttcagc
    18001 attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac gcatcacgct
    18061 gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg tatctatcat
    18121 taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc gggtcgttcc
    18181 ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca aagaggattt
    18241 accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct cttcttcagt
    18301 ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta gtccatggct
    18361 tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga tatcgtttct
    18421 catttctgga ttatctactt tattgactgc taaaatcact ggtttattac ttctgtataa
    18481 aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga ctacacaaat
    18541 gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat ccataaaagg
    18601 ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac ctaaccattc
    18661 accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa tcgagatacg
    18721 ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc caacgattgc
    18781 aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac ttagtttaac
    18841 gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt ttattcctta
    18901 ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc tttcatctca
    18961 gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct tctacttctt
    19021 ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct tctggtaatt
    19081 cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct aaagctttga
    19141 tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact ttcacttcgt
    19201 caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt tgagagatat
    19261 ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt gtcaaacgtt
    19321 taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct gaccaaggtc
    19381 caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg atagacaaca
    19441 ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct acatgactat
    19501 gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca aatgcgccaa
    19561 aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata gttgaaagaa
    19621 tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat aagatcaaac
    19681 gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct ttatagtctg
    19741 aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca cgtacaccga
    19801 cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg accttgccct
    19861 cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg cgttttgaaa
    19921 gtagatagct gccgttttct ttatctttgc cgatcgtact gataacgact aaatctaata
    19981 catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat aattcttttg
    20041 ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt ttgtcttcaa
    20101 tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg cttttcattg
    20161 cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata acacttgtcc
    20221 tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga ttattcaaaa
    20281 aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat tttttttctt
    20341 aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg caacgacttc
    20401 tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct tcagcggaga
    20461 gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca gtgtttcaag
    20521 atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac gctcacttac
    20581 gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc cgatgtctct
    20641 tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa ctaattcttc
    20701 tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg gttgacggat
    20761 ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt gttgcgaaaa
    20821 agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa aagatacatt
    20881 gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg tataaatata
    20941 attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag caggcccgtc
    21001 gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa aagccgcaaa
    21061 gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa agttgtttgg
    21121 atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct cagccacttg
    21181 ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg atgaagaagg
    21241 tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct cacttgagga
    21301 agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat cttgagacac
    21361 agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg actcctcttt
    21421 tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag ttgctgctgt
    21481 tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa tagcgataca
    21541 taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct tttgttgtct
    21601 ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt cccatggttc
    21661 ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg ttttacctcc
    21721 taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa aaatcaatgt
    21781 tccatgtttt ttttaaataa atttagtaaa atcgtttccc aatgaagttg ttcagtctta
    21841 gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc acaacaattc
    21901 tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct gcggcagttt
    21961 tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt ttcctgacgt
    22021 ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc catccatttt
    22081 ttttgcagct catcaaaggg agcttgcttt gtggcatatt ccaaataaag ctcaaaactt
    22141 tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc cccactttga
    22201 taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat ttcttgtaca
    22261 tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc agatttatcg
    22321 atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt tacaaactgc
    22381 tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc aactgtaaaa
    22441 cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca ataaatgatt
    22501 gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg ctgcgttttc
    22561 cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt tggtggtcga
    22621 tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc cgttagtgcc
    22681 agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata ttctggacgg
    22741 aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa cgcaatattt
    22801 tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact caaaaacaaa
    22861 aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga aagtaaacta
    22921 ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg atcttccatt
    22981 agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc cgtcaattga
    23041 tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc tccatcaagc
    23101 aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa atattttttt
    23161 aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt gatacaaacg
    23221 aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc actctcgcac
    23281 ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc actcgtatgg
    23341 aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc ctctttttaa
    23401 atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct gcttggtgat
    23461 catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt gccaaagcga
    23521 atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt gttgcagata
    23581 aacctctgac tcatcattgt tttttgccag ctgataagga agcagccctg ttcgctcatt
    23641 tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg aaaacagctg
    23701 caccaaactc tcatacgcag tatcaattaa aatttcacgt ggcaattggc tttcactaag
    23761 ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat agtcttgaat
    23821 acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc aactagtttc
    23881 ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat gttcggacaa
    23941 catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga tccaaccttg
    24001 ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg gaaaactgtt
    24061 atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg ttcgttttcc
    24121 cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa atagcgataa
    24181 aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc actactatgt
    24241 aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc gccagatatt
    24301 ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc tcatcaacag
    24361 ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg tccagttcga
    24421 gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg tacactaagt
    24481 tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat cccgacttat
    24541 tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg accctatagg
    24601 acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac gatcacagtt
    24661 tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc atagctgcca
    24721 aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg taaatactgc
    24781 ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt gatcgataaa
    24841 acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc catgaagaat
    24901 acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc cattacgcca
    24961 cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt gaccttatcc
    25021 gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc gactgaaaag
    25081 ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac gagagcacca
    25141 ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc aaaaaataag
    25201 taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga tggttcgtac
    25261 gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat gatgactaag
    25321 ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct gagtgacccc
    25381 tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc aaactttcca
    25441 gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg tatgtttatt
    25501 tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct ttatacatac
    25561 tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca gggaacatcg
    25621 caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct gatacaaacg
    25681 gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc actaactcac
    25741 tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc aacccatatt
    25801 tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta acagggattc
    25861 tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca aagttcatcg
    25921 ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca gttgcatttt
    25981 cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct gaaagagcaa
    26041 ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg atacttttac
    26101 caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt ttcaatgctt
    26161 cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca acattcaacg
    26221 ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa gcttgcaata
    26281 agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct tgcgtaattc
    26341 cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca tctgtaccaa
    26401 agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct ttgacggcat
    26461 acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact aaccagccgt
    26521 cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct gctggaacaa
    26581 cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct gaataaccgt
    26641 aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt tccaatactt
    26701 tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa tctgcttcat
    26761 ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt tcatgatgtt
    26821 gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag ggtcatgtca
    26881 aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg ctccatcagc
    26941 aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg agctattgac
    27001 ttatccacgt gcttatcgat agccgtttat cggtttcttc attcttatga tagaccttgt
    27061 acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac agatggctac
    27121 aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat cgaaacgagc
    27181 aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta ctaaaccaga
    27241 tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt cacccatagg
    27301 tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca agaatgacat
    27361 agggatatag aaaataaatg taataatgat gaatacactt gaagataatg aacttaatcc
    27421 ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa ccacttggat
    27481 accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac cagctaaaaa
    27541 ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga tggacattac
    27601 taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca tgcttttacc
    27661 tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt caaaaatcgt
    27721 gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac caaaagtaat
    27781 aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac gaccgattgc
    27841 ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg attttgttgg
    27901 atctttttcg atctttgatg cataacgata cacatataca atagaaatag ctaacaaaat
    27961 aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa cagcttgtga
    28021 agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt gagaccctac
    28081 taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga ctgggatcag
    28141 taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc ctaatgcaaa
    28201 taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct tcacaactga
    28261 agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc caccaacaac
    28321 caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct cccccgctgg
    28381 tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt gaggattttg
    28441 ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac tatagtgccc
    28501 agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata agaccgtata
    28561 cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct tcctttcttt
    28621 tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt atcagaacaa
    28681 aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc cctatttcct
    28741 cctctttttt caggttttta aagctttctt ctttttcatt gtagcgtttc ctttttttta
    28801 taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa gaattgattg
    28861 atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt tacattattg
    28921 catgtcttgt atttttttcc aaaaaaaaaa gaccaagaaa tttcttgatc tttttgatat
    28981 ttatttgact gctgtatgac gttgttcgaa ctgatgttgt tttcgactta accatggaag
    29041 aagttgcgca tgcagcacta aaaagacagc actcacgatg aatcctttga tcaagttgaa
    29101 aggaacgatc ccaatcagta catagtttgc taaagacatg cccagcatct gtgttgcgtt
    29161 cagccctaaa aacattaagt aaagaggcgt aataacgaaa taatttgcga tactcataaa
    29221 aatagttaga gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc ctttatgctg
    29281 tttctttaca ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg ataagaaact
    29341 agcagtgtct cctaccaaat tatccggaga aaaacctgta gtcagcagat gtaatactga
    29401 acgcaagaac gctgttgcaa ctccggccgc tggaccgaat aagaacatgc tgatcaatac
    29461 cgagatatca ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg gaaaagcaaa
    29521 atactgtaaa actaatgcga tcgcagcaaa catcgctact gcaaccattt tctgtacacg
    29581 ggtgttcttc atttgaaatc ctcctgttag gactcctctt cagcgaactt tttgacgttt
    29641 ctctccaaat gaaaaaactc tattttccag ggaaaataga gttagagtta tttgatcaag
    29701 cgtcgtcaaa taagctctat cttctttatc cagactatac tgtcggtatc ggaatttcac
    29761 cgattcagct atttttttgc aaaataggtc gtggactata accaccggtc gggaatttca
    29821 ccctgcccct gaagacgaac cttatataat ttttgtttac atattcatta tacatatcta
    29881 ttcaaaaaac gcaacatcta acacttataa aaagtaagtg ttcattttga gtggttcagc
    29941 aaactgctga cttctttttt gttcagttca cggtaatccc caggacgtag gccttgtaaa
    30001 gtcagatctc cgtatttttc acgttttagt ttttgcacag gaagaccaac tgcttgaagc
    30061 atatttttca cttgatgatt acgtccttcg tggatcgtca attccacgat actgtggttt
    30121 gtcttaggat ccactgataa aatttggaat ttagctggag atgtttttcg tccttcgatc
    30181 tttattcctt ttgtcaaagg tgcaagcatc gattttgtag cgatcccttt tacctttgca
    30241 acgtaaactt tatccacttc atgttttgga tgtgtcaggc gctgagcaaa atctccatca
    30301 tttgtcagta gcaagatccc cgaagtatcg taatccagtc gacctacagg atagatacgt
    30361 tcttttacca tcggcaaaag atccgtaaca acttttcttc ctttgtcatc tgcaacagca
    30421 gaaataactc ctttaggttt gtacaataaa tagtaaccgt attcttcttg ataaattggg
    30481 acttcatcca cttccacagt gtcatgtttc ccaactttta tccctaattc ccttacaact
    30541 tgtccgttta cttttacacg tcctgcaagg atgaattctt ctgcttttcg acgagaagtg
    30601 atgccagcgt gggcaattac tttctgcaat ctctccatta caattattct cctttattca
    30661 ttccatcaaa gaatagatct ggtgacacgt cttcttcaag tgaaatttcc atttcttgtg
    30721 tatcaggcag ttcttctaat gatttcaaac caaaataatc taagaagtag ccagttgttc
    30781 catataatat cggccgacct ggtccttcta ctctaccttt ttcttcaatc agtttatggg
    30841 cagctagacg ctgtaccgct cctgaaacct gaactcctcg aatctgctcg atttcagctc
    30901 ttgtgattgg ctgtttataa gcgatgattg ctaaggtttc gacggcggct tgagataatg
    30961 tgtttgatgt cccttgcgca tatcgcttaa gtaagggagc cagctctttt ttagtcgtta
    31021 atacataatg gctttccgtt tcaaaaatat gcaaagctga gtgcgagttt tgagtgtaag
    31081 attcttttaa ttctttcact aaacttgtga cttctgcttc gctgagattt aacagtccgc
    31141 ttaattcttc attactgatc ccttcctcgc caacaataaa aagaagggct tcaagttcac
    31201 ttaatttcgt catgttcggt atctcctact gctggataaa gcataattga ttcatagttt
    31261 tctttttgtt gcgcaatgat ctgccctttt ttcatcaatt ctagcaaagc cataaaagta
    31321 gtgacaactt cacttttaga aggtgactca aaaaaagaat cgaaagtaca gccatgcgca
    31381 tttcttttta tacgctcttc gataaatacc attcgttctt cgatcgttgt gtcatcgggc
    31441 gtgatcgttt tttccacttt ttgagaaaat ttcttacgtt ctaagatttt ctgcatggca
    31501 cggatcaaat cgattttttt gacttgtcct cgcttcaacg gcttttcagc atccgctaac
    31561 ccatcgatgt tcataggttc ttttgtgtaa taaagacttc gttcctctgc tttttctgtc
    31621 agtctttcag ctgcatattt atatttacgg tattctaaaa gctgagtgac caattcttct
    31681 cgaggatctt cttcccataa ttcttcatcg tctgtgacga attcttggac aggaagcaat
    31741 gtcttgcttt tgattgccat caatgtagca gccatcacta agtattctcc tgcaacagct
    31801 agctcgagtg ttttcattgt atggatataa ttcatatatt gctccgtgat cgtagcgatc
    31861 ggtatatcat atatatcgat ttccatttgc tgaatcagat gaagcagcag atcgagagga
    31921 ccctcgaata catctaattt aatattgatc tccgtcaact cattcactct ttcttctttt
    31981 tctttgctag ccattttcca atgtaaggct ctgtgatttc agttgcacgg atggagacat
    32041 ccgggtactt ttcctgtaat tcatctaaca tttgaaagcc catattctca ccgcgataag
    32101 acggattcaa agaaatgtca tggatgatca gctgatcgtt cgttgcccag taaataccaa
    32161 taatcccttg aatgttggtt gaaccttctg gcgtaaagaa aaaaagttca taatcgtctt
    32221 cagattcgta cgtatcaatc tccttcaaca aagaagtttt acctgtcagt ttcttatgga
    32281 aactcaatag tcccatagca attttacggt tttctttccg gtaatgtgtt aacatcttct
    32341 caccttccta tgactaagcc cttggaaaat gctgcttata aacatccgtc attcttttct
    32401 tcgtgatatg tgtataaatc tgagtcgtag agatatccgc gtgacctaac aattcctgta
    32461 ctgtccgcaa atcagctcca ttttctagca gatgtgtggc aaagctatgc cgcagcgtat
    32521 gtggtgttac ttctttgtaa atcccagcct ctcgtactaa ctgcttcaga tttttccaga
    32581 ttccttgccg agaaagacct ttcccatgat tattgacaaa tacatgcatt tcagatgcgt
    32641 cctttacaag taatggacgt gcttcgtcta agtagcgttc cagccattgg atcgcataat
    32701 cacctaaagg aatgatccgc tctttatctc cttttccaac tgtctgtacc agtccaagag
    32761 aaagatgaag atctcccaat ttcaatccaa tcaattcact gacgcgcatc ccagtagcat
    32821 acatgacttc caagattgca cgatctctta tccctaacgt ttttgtcgta tcaggtgctt
    32881 caatcagctt ttctacttct gtcagagtca atgtacttgg taatttctgt gctttcttcg
    32941 gcgtatcgat atgttgcatc ggatcatgat cagtgatacg ctcttgccgt aaaaactgat
    33001 ggaatcttct gagacttgag atcatccgag taacagttgc tgtcgctttt ttttcttcat
    33061 gcaacttctc cagaaaacgg atgactaaaa agcgatcgac atcctgccat gaagcaattt
    33121 tctgctcctg tagaaaatgc aagtactgtt ccaaatcacg ttcatagctt tgtcgtgtat
    33181 tggcggacaa tccccgttca atattcaagt agtggagata atcgttgatt tgctctttca
    33241 taataatccc cctcatgcct ctctatcata tcagataccg cttcattctg atagtctttt
    33301 tccataatct gagcaaagaa aaa
//
LOCUS       DAZKCY010000020        32558 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000020 DAZKCY010000000
VERSION     DAZKCY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.020"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..284
                     /locus_tag="ACG8XC_000844"
     CDS             15..284
                     /locus_tag="ACG8XC_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190561.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGINNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLSELEQV"
     gene            289..453
                     /locus_tag="ACG8XC_000845"
     CDS             289..453
                     /locus_tag="ACG8XC_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190562.1"
                     /translation="MDLKTFTAQIELMHQEALRQSASYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
     gene            446..748
                     /locus_tag="ACG8XC_000846"
     CDS             446..748
                     /locus_tag="ACG8XC_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190563.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKK"
     gene            745..1056
                     /locus_tag="ACG8XC_000847"
     CDS             745..1056
                     /locus_tag="ACG8XC_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFP9190564.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            1053..1271
                     /locus_tag="ACG8XC_000848"
     CDS             1053..1271
                     /locus_tag="ACG8XC_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HFP9190565.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            1282..2097
                     /locus_tag="ACG8XC_000849"
     CDS             1282..2097
                     /locus_tag="ACG8XC_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFP9190566.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            2236..2592
                     /locus_tag="ACG8XC_000850"
     CDS             2236..2592
                     /locus_tag="ACG8XC_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFP9190567.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKAKERAELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKAHSIQHGFI"
     gene            2552..2797
                     /locus_tag="ACG8XC_000851"
     CDS             2552..2797
                     /locus_tag="ACG8XC_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190568.1"
                     /translation="MIKKLIQFSMDLYDIESGATVSVESDHLIISFADERQIIIWVVD
                     DMLYPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            2794..3192
                     /locus_tag="ACG8XC_000852"
     CDS             2794..3192
                     /locus_tag="ACG8XC_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFP9190569.1"
                     /translation="MIPKFRAWDKRENTMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLG
                     HRNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            3189..3527
                     /locus_tag="ACG8XC_000853"
     CDS             3189..3527
                     /locus_tag="ACG8XC_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190570.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            3524..3754
                     /locus_tag="ACG8XC_000854"
     CDS             3524..3754
                     /locus_tag="ACG8XC_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190571.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            3761..3964
                     /locus_tag="ACG8XC_000855"
     CDS             3761..3964
                     /locus_tag="ACG8XC_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFP9190572.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASETIRKFGISILNDDRKWEAER"
     gene            3961..4254
                     /locus_tag="ACG8XC_000856"
     CDS             3961..4254
                     /locus_tag="ACG8XC_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190573.1"
                     /translation="MKLKDGFYTDAYGIGGLMMDLPTKNPIKQEESEIKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKRLAKSKENLAVVRLVDMEVIKS"
     gene            4323..4724
                     /locus_tag="ACG8XC_000857"
     CDS             4323..4724
                     /locus_tag="ACG8XC_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP9190574.1"
                     /translation="MNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTK
                     NLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLG
                     YSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            4799..5266
                     /locus_tag="ACG8XC_000858"
     CDS             4799..5266
                     /locus_tag="ACG8XC_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFP9190575.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(5666..6019)
                     /locus_tag="ACG8XC_000859"
     CDS             complement(5666..6019)
                     /locus_tag="ACG8XC_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190576.1"
                     /translation="MAKIDDYRSDMIEYIELFAKNSNNNIIIQTGGATICGTPIDFDA
                     EVKVNPLIDAMMDSFAEFRSKKIDDIEKDDEQSLVVKSIFLKDVTIIGERTTNIPFLV
                     VFADQISAISLGNLE"
     gene            6566..6778
                     /locus_tag="ACG8XC_000860"
     CDS             6566..6778
                     /locus_tag="ACG8XC_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190577.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQSKYFYDQL
                     ILQGFNEGQAMEFMMRTFSASNQQKE"
     gene            6785..7012
                     /locus_tag="ACG8XC_000861"
     CDS             6785..7012
                     /locus_tag="ACG8XC_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190578.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIIEMTREAT
                     PNEIDKYNLRYCGHGYWKDEYIQQNIERYIK"
     gene            7009..7293
                     /locus_tag="ACG8XC_000862"
     CDS             7009..7293
                     /locus_tag="ACG8XC_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190579.1"
                     /translation="MNDNYDYIKLIEKIRAEKDMDELANLFMNIISLVGLKMDEVAAL
                     NYFIAEQTIRAEHNAKFLKDRLDLDVKGLGVEGIFKVQEALVNVYVEKMQ"
     gene            7334..7906
                     /locus_tag="ACG8XC_000863"
     CDS             7334..7906
                     /locus_tag="ACG8XC_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190580.1"
                     /translation="MKLKDYIREGYNIVTTPNLAYKIQEDYPNVLVFTDRALDAIPIG
                     KLLVDRYYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWHTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            7907..8218
                     /locus_tag="ACG8XC_000864"
     CDS             7907..8218
                     /locus_tag="ACG8XC_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9190581.1"
                     /translation="MIDVSTKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            8212..8469
                     /locus_tag="ACG8XC_000865"
     CDS             8212..8469
                     /locus_tag="ACG8XC_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190582.1"
                     /translation="MVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEETTTVEDI
                     SINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            8673..9191
                     /locus_tag="ACG8XC_000866"
     CDS             8673..9191
                     /locus_tag="ACG8XC_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9190583.1"
                     /translation="MKQKRIEKGLTQADVATKSGISSRYYGSIENGKNSPSIETLNSI
                     SIVIGCSLHFLLNDRMDDMEKRVELEKKRLQKIFDKIPHDKLNLVEGLIIQAARLRVL
                     LDDNWKDIIENGEYEKFKQSENQIAYDRKRPIVENYDNRDKTYQAIVKQLTDLLPQGT
                     RADKKSKLLGRK"
     gene            9194..10885
                     /locus_tag="ACG8XC_000867"
     CDS             9194..10885
                     /locus_tag="ACG8XC_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP9190584.1"
                     /translation="MLFNQHVDQYIRKWKSGELILNEKRIQLLKLIEKEILPRDDIYY
                     FDEEQINNYIEFSQAWYFELDEWEKFISAFIFLFYKEDDEVVFDEFVINMGRGGGKNG
                     FISTLANYFVSSLHGIDYYDVSIVANSEKQAKRSFQECYRVINKKGNEDLKEEFEAYK
                     SSLTGLETQSVFEYKTSNASSQDGGREGAVIYDEYHEMENTDIVDVFSGGLGKVDHGR
                     QFFIGTKGFVREGYFDIKYRECEDILNGLSEFKGVFPYICELDAIEEMDDPKNWPKAN
                     PALQPPLNKRGKRLFNEVMKKYKKLAKEPSGRSAFVTKRMNFLEDNMENSVASREEIM
                     ATNRPFFELDTVPIGSLDFGSVRDFATCGLLFKNRKKSEYAFKSFTFAMKNFCDVHYG
                     YSLKEQLVGSEKRAPIKTWEKMGLMKVIDEPSLNPKYIVDWFIQAREEYGVRVIVVDN
                     YKADILGPMLEKEGFEVVRLKRPSSLHPLLAPRIEDGFANHKFIFGDNPLMRWFTNNV
                     YVKETQAGKQFLKKEEVKRKTDGFQAFVHALYKATELDEQIDYDDAFDMLDEIEF"
     gene            10917..11783
                     /locus_tag="ACG8XC_000868"
     CDS             10917..11783
                     /locus_tag="ACG8XC_000868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFP9190585.1"
                     /translation="MRIKGKNISWVFYMLGILAFTFVAIKLSEIIIPNDSVVSKNLVL
                     ERAKLIISILGPALTLIVFVRTIQTQLESSKNLIKDNVSKDFYNLLNLFKSIQDKMNS
                     ELFSTIEKRIKVPINNINTNLDVRENKETTKEVQEDLKEYSASLNKVLSESVKKINEI
                     FEDNYKDIGSYLKVFHRILKILNNRYESKDISHEEYKMYVGILRSQIEDYEFYIILIN
                     SLFTLRGTGMGIELIETSFFGDELDIDLKQHFEHPEFLTEIIKDNFVEKKSYRESVKT
                     RKKIENYLKIER"
     gene            11912..13084
                     /locus_tag="ACG8XC_000869"
     CDS             11912..13084
                     /locus_tag="ACG8XC_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9190586.1"
                     /translation="MSLFDIFKQSIKNEDPSDWIPDFVFGDEESARAYLKIMAKSTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWAMSDVIYMEYNNEELERFTR
                     GLFEDYAELFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDKIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIADVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            13071..13775
                     /locus_tag="ACG8XC_000870"
     CDS             13071..13775
                     /locus_tag="ACG8XC_000870"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFP9190587.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            13780..14940
                     /locus_tag="ACG8XC_000871"
     CDS             13780..14940
                     /locus_tag="ACG8XC_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9190588.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTVEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVEYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            15016..15294
                     /locus_tag="ACG8XC_000872"
     CDS             15016..15294
                     /locus_tag="ACG8XC_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFP9190589.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            15275..15646
                     /locus_tag="ACG8XC_000873"
     CDS             15275..15646
                     /locus_tag="ACG8XC_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFP9190590.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            15662..16066
                     /locus_tag="ACG8XC_000874"
     CDS             15662..16066
                     /locus_tag="ACG8XC_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190591.1"
                     /translation="MSNVKFEGVQETIQALEKKFGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIDEVVRKNATYRNYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSEPLYFDAVGEEVKKRL"
     gene            16063..16473
                     /locus_tag="ACG8XC_000875"
     CDS             16063..16473
                     /locus_tag="ACG8XC_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190592.1"
                     /translation="MKDMLNIIYEALCLNEYIRSMTYNENTEQYRIKFYEQPETADKT
                     GPMITLRPVDTPNEAYHGSNKELSVEHMVQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFSETKRFVDARRYEGNTKIYDTEY"
     gene            16534..17121
                     /locus_tag="ACG8XC_000876"
     CDS             16534..17121
                     /locus_tag="ACG8XC_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFP9190593.1"
                     /translation="MTLVGFKKMTIGIFDSTGKIPAANLYVIEGEQDKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITALKEMVFPAAGE"
     gene            17194..17661
                     /locus_tag="ACG8XC_000877"
     CDS             17194..17661
                     /locus_tag="ACG8XC_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFP9190594.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            17707..18015
                     /locus_tag="ACG8XC_000878"
     CDS             17707..18015
                     /locus_tag="ACG8XC_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190595.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            18210..24974
                     /locus_tag="ACG8XC_000879"
     CDS             18210..24974
                     /locus_tag="ACG8XC_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9190596.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGITGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRDLATKLPASHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKMLKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKALDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDNTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPKV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDIVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPNDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPNAKGTNFHPG
                     GAAMVNDQKGPTYEELITLPSGEAFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYADG
                     IGNITTVSSTLNLDALILAINELTTVLRTQLMAETTNAKDTKLSDETKVADPIIPGSL
                     SEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGVAW
                     NKNLLNGWNSLTGTFISTINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYGS
                     FIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMVG
                     GMGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHEE
                     YVQMPDGRGFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDLL
                     DDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDGA
                     VNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRYT
                     AAANYAFEKFPSKIAYVITNGRVRDRMGLSGTGSSGQWTNWPDGDHFDHIHLNGSMGS
                     GDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGND
                     GLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIGK
                     GHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGDDKKRSNTT
                     NTADNSAELVTLIKQQQKQHNELMMILRAILGKDLSLKSSDIGQAANHYMGSDLNKLR
                     YTNGGV"
     gene            24978..25799
                     /locus_tag="ACG8XC_000880"
     CDS             24978..25799
                     /locus_tag="ACG8XC_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail domain-containing protein"
                     /protein_id="HFP9190597.1"
                     /translation="MFYKLQFNQNGKLFDPQRKEKIVCKEIKRQAPVYEVNYEDFEGT
                     NGSREANASFRPFELVLTFDIFYKNKHDKELLLTEFYELIFVGYQYYISYDLSPGKRF
                     KVNPTNFEITEEENDYSTIEITFSIPSGSAESSATTLTDFNLEEEWQFSQGLVAEDYK
                     YTHQTSHFIIYNAGSFEIDPREHYLRIALEGESEGNVTIFNKTTGDRFIYYPSLSTNL
                     GQTLVLDGVIPKLNGVSCGINTNHGLINLVEGVNEIEIQNITRVKSSWDFRFLYK"
     gene            25808..26851
                     /locus_tag="ACG8XC_000881"
     CDS             25808..26851
                     /locus_tag="ACG8XC_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9190598.1"
                     /translation="MTELIIRNYEQTKEEILVGYDKGSFYENWQQNETWEIGFTVTSN
                     SLNQEVFDLVEYESSVFYNGQEFVIKEMTRKALGQLLTKQVVATHIYYTVQDGYQYNT
                     VTGAKSISQLLTHVFSAGSRGFTWEVIDPNKKFLTVEQENFGNANYLKLINEILSDYN
                     AVVIPNNKHLTFYPASEYGQQTEEQIRYKYNTDEVSFDIDTYSLKTQIKGYGKLKDGA
                     NTENPKDSDYIFTPITYTSPESQKWGIRIQDPVKDERYTVSGNMLERLKTDLQDYPNI
                     SGSVTLKWKISPNKGDHVPFIYEPLNINTYIQVVGIKTYPAIPNKPPEITLSNTKKTM
                     TSILAEMAKKGVI"
     gene            26851..27882
                     /locus_tag="ACG8XC_000882"
     CDS             26851..27882
                     /locus_tag="ACG8XC_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190599.1"
                     /translation="MGLLKLISNRISTEWKEKFNKNIDYLNDLEKKLSDQDKSMNSRI
                     DNLVLHSGGESPNEVVDARVNNKGETFPTLHGRLVEHETLTDEQISELNTNMDSQKEQ
                     VQQLNKSVQQIIGGYNEPINIYVSKDGSDILGDGSEEKPFLTIQTAVNNVPLITTSQI
                     TIWVGSGAYLEDIMIRNLNFTSFLIRPIDNFDTIDPLKSDLPVKIRSICFTACKGYCQ
                     VAGMQIVDTANGADYGIRNEQSGYMAINRCKFAENTKTLARYNAVYVGGTSKINMYGD
                     TTLINQKVAIYAVLMGEIFVSAFGSGNDVGILCENGTVRGTVSTSFATTPTKTTGYGL
                     IITKGTVLQ"
     gene            27882..29984
                     /locus_tag="ACG8XC_000883"
     CDS             27882..29984
                     /locus_tag="ACG8XC_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFP9190600.1"
                     /translation="MVYKTNESIIMIQAEATSPNRTNVVFWSHDRGTAKLRMKLVRKN
                     GIPQSLPEGTTVPIRLMLKSATAEGGYGKHDYLATIEDRVNGIVSIVLEDNILGYVGI
                     VEGSVYIDFPNDRSLDTAGRFTFDIKRSPIDDSTPELEDYYFNGFSQTIDKIEKILAD
                     GKQEIEQKITESETQIDGKLKETSNKITKANQDVATINTNIDKANDRIDQTNQQISDL
                     GKLKKMYSNSIDFGNYDYSGNPNLMANINADSFSQGSGALSVVDDGDEVVITLDPNHK
                     LEIFKAKSQPALVVGKTYTMSVEIMLEDDFTGDPSNISLRYIKMPSWLTVLYTPYTLT
                     NTKGVWQKLTVTAKMTTAIDNAESWFIMLQNKDANNSLSGKLRLRHAKLEEGSTATPF
                     QPNLLAEPYNMCREYPNENIADPKVKFPIESGDHQIYQGYTEEELMIGQTYTITLKGT
                     KPASQTFVAYNHWTARLGELKPVDGLTDVWSLTFTPTNVVAMPKLFRVYQYPRSTVGA
                     CQIDWLKIEKGNTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKGLNNAVNVYD
                     PQRVEGLKNFADGIQIAGDKVISENDCTVYTLTKDNSQSFIDGYATFIKHGKEVVVNG
                     TVKFKKAYAFGVPLDDEVPDEFIARIVHGMLTGQSGTNSVSKAMYVQKDLGKIMTNSE
                     FAANEWFTFHGNYWVGVK"
     gene            29985..30431
                     /locus_tag="ACG8XC_000884"
     CDS             29985..30431
                     /locus_tag="ACG8XC_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190601.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGLRADGEPL
                     TIADQMFDPKLNQWIVLANTLDHNDLNNLKAMYEALEHENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPTLKGGE"
     gene            30433..30570
                     /locus_tag="ACG8XC_000885"
     CDS             30433..30570
                     /locus_tag="ACG8XC_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFP9190602.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            30605..30847
                     /locus_tag="ACG8XC_000886"
     CDS             30605..30847
                     /locus_tag="ACG8XC_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFP9190603.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKNNNKWAWGYMIGLGITIIGYFLTKL"
     gene            30863..31060
                     /locus_tag="ACG8XC_000887"
     CDS             30863..31060
                     /locus_tag="ACG8XC_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFP9190604.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMIVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            31071..32090
                     /locus_tag="ACG8XC_000888"
     CDS             31071..32090
                     /locus_tag="ACG8XC_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9190605.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQIA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            32280..32353
                     /locus_tag="ACG8XC_000889"
     tRNA            32280..32353
                     /locus_tag="ACG8XC_000889"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32314..32316,aa:Ile,seq:cat)
BASE COUNT        11629 a         5071 c         6965 g         8893 t
ORIGIN      
        1 taagaggtgg aaaattgaaa atcgattatc tagaactaat taatgaaata gcaaagtata
       61 aaactggtga ggaaatagaa atcctgagag acgtatatga tcaactcgaa gaagctggaa
      121 tcgaaggaat taataatgat cgttcgagtt ggagtaaact cagatactat ttcgcactct
      181 atatcgatac aacacaatta agaaatttag cttatacaaa attactattt gttgattgcg
      241 ttaaaggatt gcaaaaacat cttagtgaac ttgagcaggt gtaatcagat ggatctaaaa
      301 acatttacag cacagatcga actaatgcat caagaagctt taagacaaag cgcctcgtac
      361 gaagacaagt ggctcaacac gttccatggt ggacgtgaga gcgcacttga tcaagtactc
      421 aaattattga aaggggaacg tcgggatgga taagaaagcg gcaatgaaac gaatcatcga
      481 actgacacat tctgagaatt ggcaagaaga caaagaaata gttacagaag tccaaaagct
      541 cggtaaatca atgtggactg aaaagcccaa acggagaacg ccgagaaaga ttgcaatttg
      601 gcatggtgat cgaattctag taacaggtac tgctgaacag ttatctgaaa ttactggtct
      661 gagcaaaaac atcatctggg atagagcaag gagcttatgg attgattcaa aaggacgaca
      721 atttaggtat gtggaggaga aaaaatgaac gaactaatca caaaagtaga gcagtgggct
      781 aaagacaagg gactggatca agcagatcca aaagcacagt ttttgaaagt agctgaggaa
      841 ttcggggaaa tcgcttcagc aatggcaaga agtaatgatg agctatttaa agatagcgta
      901 ggagacgtaa tcgtcacgtt gattatcctt tccatgcaaa aagggacaaa cgtacaagag
      961 tgtttagaaa tggcatacaa cgaaatcaaa gggcgcacag gaaaaatggt agatggtgta
     1021 ttcgtgaagt cgagtgattt ggaggacagc aaatgatagg tttaacaata ttttggtgtg
     1081 cgattggtgg tttactagca tttctaggat actacctcat ctgtgaaagt agaaaaggaa
     1141 gaaaatatac agtgacaggt attgtcctgt taattatcgg aattctagtt gcagttggag
     1201 cagaactgaa tctttatgta aatggtacac aagaagattt ggtgcgattt atgttttgga
     1261 tgaagaatta ggaggaaaaa aatgaacgaa aacaaattaa tcaaattagg tgtagcagga
     1321 gcagtaatcg taggtattgg agttatcgga ggatttaagt tcttcgaaaa aatcgataat
     1381 ggatatgtgg gtgtgcgcta ttcaatgaac ggcggtatca aagatgaagc actgacgcaa
     1441 ggtgtgaaat ttgtagggat tgacaaagtg atccaatatc caattcgctt gcaaactatc
     1501 caatcaaaaa atatttcagt atctacaagc gacggcaaaa agacaacgat tgatatcaaa
     1561 tatgactaca aagttgattc aactaaagca gcaaaaatgt acaaggaatt tgggaatatc
     1621 acttcggaag atatcgaaag tggatggtta aaatctaagc ttcaaaaggt cgctcgtgaa
     1681 gtttatgcga aatatagtct gcttgatgtc ctttcaggag attcctctaa agttgaagct
     1741 gaggtattaa cgaactttgc taaatcagtt gaatctaaag ggtttgaagt cgaagacgta
     1801 acacttggtg ttccagatgt cgataaagaa acacaaaaat caatcgatgc gatcattcga
     1861 gctggtcaag aaaatgaaaa agcgaagcta gatgcagaaa ctgcaaaaac tcaagctgat
     1921 agtgaagctt acaagaaaac aaaagctgca gaagcagagg cagaatctaa tcgcaaagtc
     1981 gccgaatcag taacagacaa tttgattcgt tatgaagaag ctcaagctcg caaaaagcat
     2041 ggatgggtaa cagtaaatgg agcagatact gtagttacgg atgaagcagg caaataatat
     2101 gggattcttt atggctaaaa ttctcttgtt cttaggttta gtaggagcag catatctcgt
     2161 gtatgcgctc ctttcccaaa ctgatgacaa agaagatgac aacaacgatg aaatgaaatt
     2221 ttaggaggag aaataatgga tctcattaca caatacagtg atatcatcct caagaaaatc
     2281 atggcaaaga ttcagaaaga taaaaaagca aaagaacgag cggaattagt taagttggaa
     2341 atggctgaaa caggagtagg agtgcgaagt agcaggcatt ggaaagcagc agcaaacatt
     2401 gaattttatt acaacgaaat tcaaaaaggg ttcgatcaga tgcgtgagct ggatcggcaa
     2461 acaaattgga gcaagaaact tcatcaagat cgtttcaaat ttgtagagaa gtataaagaa
     2521 atattagaag agtatttgag gaggacagcg aatgataaaa aagctcattc aattcagcat
     2581 ggatttatat gatatcgaat caggagcaac agtatctgtg gagtctgatc atttaatcat
     2641 aagttttgct gacgaacgtc aaattatcat atgggtagtt gacgatatgc tatatccaga
     2701 aattgtacat gattttgaag aatcaaaagc ggttgagttt gaaatagtga aaaaagtaat
     2761 ggaattgatt gaaaaatacg aggaggacag caaatgatac cgaagtttag agcgtgggat
     2821 aagcgagaaa acacaatgag agatgtagct gtcttacatt ttactaaaaa cggcaaaaca
     2881 aactttattg aatattggat aaatcctacc gaattgaaat catatcacgt gcgaaacatc
     2941 gacctcatgc aatccacagg aatgaaagat aagaatggtg tggagatatt tgaaggggat
     3001 gtagtaatta catcaaaagg agcgattgga tatgttgcgt atcttcaaca agaagccggc
     3061 tttgtagttg tactaaagaa aagtgattat agattaggcc ataggaatac tggcgaaagt
     3121 tatgatgtag caactgctca tgaagtcatc ggaaatatat acgagaatag cgagttattg
     3181 gaggtagagt gattatctta gctactgact acatcgattc tttaaaagat gaaaatggga
     3241 atgtcccagc attgctaaat tgtggaatta aaagcaagaa tagaatcatt ttgttacgga
     3301 tatcggctga tttagcaaaa tatctttatc tgagcaagac agcagtatat tacactgcgg
     3361 ctacacgtta tcagtacaag ggaaaaacaa tcagtcagga ttattatgat cgtttaatca
     3421 gtcctgacat gcatggaaaa tcaaaatcag cgatcaagtt atttggcgca atagagatat
     3481 atgccgatga ttttccaaat ttatggttga aggaggaagc ggaatgaaat acgaaatacc
     3541 actaagtgaa gcgggcattc aagcaattat caatggtcgg gaggttaata aaaaacttcc
     3601 tgatggtact gaattagtca tcagacaaag ttatttgaaa gatatggcag ctccagtatt
     3661 aattgatcgt tttaacgtga ctgattctgt ggtagagaac cacttgaaag aatttcgatc
     3721 aagtataaac gacactttca gattagggag ttgattgaca atgaacacca gacaccgcag
     3781 agtagcaaaa ctaagaaaac aggaactgaa tgtactaaag acaaagtttg aaaaagaata
     3841 tggaatttca gcagaagaaa catataaagt ggcaagtcag tttgttgctg atgcgagtga
     3901 gactattcgt aagtttggga tttcgatatt aaatgatgat cgtaaatggg aggcagaaag
     3961 atgaaactaa aagacggatt ttacactgat gcttatggaa ttggtggttt gatgatggat
     4021 ctaccaacta agaatcctat aaagcaagag gaatcagaaa ttaaagtcgg tgacatggtt
     4081 cgctgcgaag cagaagggtt catctatcca tttcgtggat atgtagagca tctctataat
     4141 cactcagcga tcattcgcat tgaaaacacg atggaatgtg acaagcggtt agcgaaaagc
     4201 aaagagaatt tagcagtggt gagattggtg gatatggaag ttataaaatc ttaattttgc
     4261 ttttttacaa ctatcctaca acaatgagcg ttattccaca actggaagga gaagaggcaa
     4321 ttgtgaacat tttagagaat atagatatta agcaaacaag gaaaaatgct agacgattac
     4381 taaaaagata cagaaattta gaacgtttgg tcggaccagt gaaaatagac ttttccgtga
     4441 tgactgttac caaaaatttg aaattcacaa ttgacagtca aaacgaagaa atcattgaag
     4501 cgataagtac tcgagattcg gttatcgaag cattaacacg gctaagtaga atccatttcc
     4561 aggtacttta ttatagttat tgttttccta ataagatgtc aatgtatcaa ataggagaaa
     4621 aattaggata ttccgataga actatcgaga gaatgaaggc agtagctttg gttgaatttg
     4681 ctgaggcgta taaatcagga gaactcattt cacgtacaaa ataaaaaaag tcggattcct
     4741 ccgactatga ataatatttc cgacataagt attatatcat aattggggga atcagaggat
     4801 ggtacttttc gatgtaaaga aatatgagac accaagcgcg aaagatgttg atatggaacg
     4861 cacaaaacat aatgtcgctg tgtttctttc agcatatcta tcagctagat gtagagtagg
     4921 gcaacctcga gaaccaaaag tgacagcatc ttattccttg gttccacctt ctacagctga
     4981 tcatatattt gaagcagaaa gaatgctgat cgataaagaa gaagcacagg aagaatttga
     5041 gtatctgcat aaattgtttg ttcgaggcta ttcagcgata cagcatccgc ataagcctga
     5101 tgtgactgaa aggcgcaaga agatattcta tgatcgttat atcaatggtc tgcccattta
     5161 tgtaactgct caaaggaata atactagcga agaatcggtt aaagtagaat caaacagaat
     5221 tatcatccaa tttgcttcat cgttagaact ggttgctttc aagtagccag tttttacact
     5281 ttttataccc ttttattacc aatttggttt ccattttata ccttttttat accaatcact
     5341 tacctattca acgttgtatt atgatagtgt cgaaagatta gtgataggtc ttagacaaaa
     5401 taaaatgtaa gggaggaaat ctccctcatc gttttaaatt aagcctcgat agacagcagc
     5461 ggaaatatta agaataagga tgtgaactca actccttctg aattcttcgt atgctgctgt
     5521 ctattaaatt atgtgttgga gggtaagaat atgaaattat cgattgaagg tacttcagga
     5581 gaaataaaag aaatcctctc agctatttgt aatagccgag agattagcaa tttaatcaat
     5641 atagataatt taaatgaatc tattgttact ccaagttccc gagagaaatt gcagaaatct
     5701 gatcagcaaa aacaactaaa aatgggatat tagtagttct ctcgcctatg atggtaacat
     5761 ctttcagaaa gatagatttg actaccaaac tttgctcatc atctttttct atatcatcaa
     5821 ttttttttga gcgaaattct gcaaaagaat ccatcatagc atcaataaga ggatttactt
     5881 ttacttcagc atcaaagtct ataggagttc cgcaaattgt tgctcctcca gtttgaataa
     5941 taatattatt gttagaattt ttagcgaaga gttcaatata ttcaatcatg tctgaacggt
     6001 aatcatcaat ttttgccatt tttttaccac cttaaattat ttcagcggac cactcgctga
     6061 taactaaaat tatacgctta gtatttattt tcacaatatt aatttgtcac tgtggcggaa
     6121 aggtagacgc taagcatgcg tgctaggtca atgcttcggc aaccatgcaa tgttcgattc
     6181 attgccagtg acttaaggaa cctacggaaa caattcatct tatcggatgc cgatgaattg
     6241 gctgactagt cgggatgcca ctagcagtta aaaggcataa aatactagcg cagacgtgcg
     6301 ccactctcag gtgtaggtta ggagagaaac attaattggg gttattagga atacgataac
     6361 ctgcttgcga caaagctttg tactgtcgcg ttggtcatgg acagagacgg tattctgttt
     6421 cagtattcgt tagcaaccga agaatcggcg gtttaaaaat ataggggtgc gcaatttcgt
     6481 acgcgttttg tgcatcgtgt aggttgctat tacatattag atcactcatc gagtgatctt
     6541 tttattttga aaggggagta aacaaatgaa cgaaaaccaa ttaagagagt tgtttaaaac
     6601 gaatgaagca aacaaaacaa tggaggcgac attctacgaa actcaaaaaa gcttagcgtt
     6661 aatcgcaaaa caatcaaagt atttctacga tcagcttatt ctgcaaggat ttaatgaagg
     6721 acaggctatg gaatttatga tgcgaacctt ttctgccagt aaccaacaga aagagtgata
     6781 cataatgaga aactactggt atatatcgct aactaatgaa tatcctcgaa ccattgatga
     6841 ttgttcagtg cgtgttgtgc gttctgtaca aatcaaaaag aagtactcca tcattgaaat
     6901 gaccagagaa gctacaccaa atgagatcga taagtacaat cttcgttact gtggccatgg
     6961 atattggaaa gacgaatata ttcaacaaaa cattgagagg tacatcaaat gaatgataac
     7021 tatgattaca tcaagttgat tgaaaagata agagcagaaa aagatatgga cgaactagct
     7081 aacttattca tgaacatcat tagtttggtg gggttaaaga tggatgaagt agcggcacta
     7141 aattatttca tcgctgagca aacaattaga gcagagcaca atgctaagtt cttgaaagat
     7201 aggttagatc tagatgtaaa aggattaggc gttgaaggga tattcaaagt gcaagaagcc
     7261 ttagtgaatg tttatgtcga aaagatgcaa tagcattaac aagtaattat tggatgtttt
     7321 caggagggaa actatgaaat taaaagatta tatcagggaa gggtataaca ttgtaactac
     7381 accaaatcta gcttacaaaa ttcaagaaga ttatcccaat gttttagtgt ttactgatag
     7441 agctttagat gccattccta tagggaagtt attagtagat cgttattata gcaacaatcc
     7501 agctgttctt agtgctaagc cacttcaaaa cgcttatgct attgaacgct ttacttgcga
     7561 gtttactgga tatgagacgg tagtaccaat gacaggcgag acaggaagaa aagtaattca
     7621 acagatcaaa aagaggattg ctgaattgac gttagatgat ggaagtaata cgaaccttct
     7681 agaaatcaaa ctaagagata ctgactcagt accagaagtt tattataaag gtcaacgaat
     7741 ggatgagatg ccgataggtc ttgtcgatat tacttatcat tggcatacgg aagaatatga
     7801 atgccaagta ccaaaaggag ctaacgacat cactattgaa tattatgatt cacataataa
     7861 caagtactta gatagaaaaa tcattgggca caagagagat atgtaaatga ttgatgtttc
     7921 aactaaacaa gcaagagcta ggttctatgg atcaactgag tggaggaaac ttagaagtca
     7981 atgcttagag cgagatcatt atgagtgcca atggtgtaaa gcagagggta gactaacaac
     8041 tcaacatgat tcaattcttg aagtggatca tataaaggag ctggagtatc atccggattt
     8101 agctctagat ataaacaacc taagaacctt atgcaaagat tgccataaca aaaggcacaa
     8161 ccgtatgaaa tatcgtggcc aacaaaagaa aagaaagtgg gatgatgaat ggtggtaaga
     8221 aaaacagcaa acagagatga tgtcaaacga gcagaagcaa tcgctatggt tgataaggct
     8281 attgataagt tggttctatt taagagtcag ctcattgaag aaacaacaac tgtagaggat
     8341 atatctatca acaatagact aataggtaac gctaatgaag taacagggac atttaatatt
     8401 aatgtagaca ttgattatac cttgagtcca tttgcacata ttgtagaaag caacgagcaa
     8461 gacggctgat aaatcagtat aaagcgaaga tatatagagt gaaatacccc cggtcaaaag
     8521 attttgagaa gaaataaaat ttttgggaac cggtggatgg ggttaattct gcaaatgcaa
     8581 cgtatttatt ttacccctcc cccctccgcc gcccctttag taaatataga aggaaggtga
     8641 ttggtttgaa ttctaagttg gttggcacga gaatgaaaca aaaaagaatt gaaaaaggat
     8701 tgacacaggc tgatgtagca acgaaaagtg gtatttctag taggtattat ggaagtattg
     8761 aaaatgggaa aaattcccct agtatcgaaa cgttaaactc aatttccatc gtcattggat
     8821 gcagtttaca tttcttactc aatgatcgca tggacgacat ggaaaaacgc gtagagctag
     8881 aaaagaaacg tctgcagaag atattcgata aaattcctca tgacaaactt aatttagttg
     8941 aagggctgat cattcaggca gcaaggttac gtgtcttgtt ggatgataac tggaaggaca
     9001 tcattgaaaa cggggagtac gagaaattta aacaaagcga gaaccaaatt gcttatgata
     9061 gaaagcgtcc tatagttgaa aattatgata atcgagataa aacttatcaa gccatcgtca
     9121 aacaactcac cgatttatta ccgcaaggca caagagcgga caagaaaagt aagttactcg
     9181 gtaggaaata aaaatgcttt ttaaccagca cgttgatcaa tatattcgta aatggaaatc
     9241 tggtgagtta atccttaacg aaaaaaggat acaactttta aaactaatag aaaaagaaat
     9301 attacctcgc gacgacattt attacttcga cgaggagcaa ataaataatt atatcgagtt
     9361 tagtcaagca tggtattttg agttagatga atgggagaaa tttatttctg cattcatttt
     9421 tttgttttat aaagaagatg acgaagttgt ttttgatgaa tttgttatta atatggggcg
     9481 tggcgggggt aaaaatggct ttatttcaac attggcaaat tattttgtca gttcgcttca
     9541 cgggattgat tactatgatg tttcaattgt agctaatagt gagaaacaag caaaaagaag
     9601 ttttcaagaa tgctatcgag taatcaataa aaaaggaaat gaagatttaa aagaagagtt
     9661 tgaagcgtat aaaagcagtc taaccggttt agagacacag tctgtttttg agtacaaaac
     9721 tagtaatgca agctctcaag atggcggtcg agaaggtgca gtaatatacg acgaatacca
     9781 cgaaatggaa aacacagata ttgtagatgt gttttctggt ggactgggga aagttgatca
     9841 cggacggcaa tttttcattg gtactaaggg gtttgttcgt gaaggttact ttgatattaa
     9901 atatagagag tgtgaagata ttttaaatgg tttgtcagag ttcaagggtg tatttccgta
     9961 catatgcgaa ctggatgcca ttgaagaaat ggatgacccc aaaaattggc cgaaagcaaa
    10021 cccggcactg caaccaccgt tgaataaacg tgggaaacgt ctgtttaacg aagtcatgaa
    10081 aaagtacaaa aagttagcta aagaaccttc tggacgctcg gcatttgtta ctaaacgaat
    10141 gaattttctt gaagacaata tggaaaactc tgttgcttca agagaagaaa taatggcaac
    10201 aaatcgtcca ttttttgaac tagatacggt accgattgga tctctcgatt tcggtagtgt
    10261 tcgagatttt gctacttgcg gtttgctatt taaaaatcga aagaaatcgg aatacgcgtt
    10321 taaatcattt acgtttgcaa tgaaaaattt ttgcgacgtt cattatggat actcgttgaa
    10381 agagcaattg gttggcagtg aaaaacgggc accaataaaa acttgggaaa aaatggggct
    10441 gatgaaagtt attgacgagc catctttaaa tcctaagtat attgttgatt ggtttattca
    10501 agctagagaa gaatatggag tgagagtaat tgtcgtggat aactataagg cagatatttt
    10561 agggcctatg cttgaaaaag aaggatttga agtagttcga ctaaaaagac catctagttt
    10621 acatccatta ttagctcctc gtatagaaga tggatttgca aatcacaaat ttatttttgg
    10681 agataatccg ttaatgcgtt ggtttactaa taatgtgtac gtaaaggaaa cgcaagcagg
    10741 caaacaattc ttaaaaaaag aggaagtcaa gcgtaaaact gatgggttcc aagcatttgt
    10801 acacgcattg tacaaagcta cagaattaga tgaacagatt gattacgatg atgcttttga
    10861 tatgttggat gagattgaat tttaaggtat acttttcttt acaaaggggt gctttcatga
    10921 ggataaaagg aaaaaacata tcttgggtat tttacatgtt ggggatttta gcttttacat
    10981 ttgtggcaat aaaactatca gaaattatta ttccaaatga cagtgtagtt tcaaagaatt
    11041 tggttttaga aagagctaaa ctaattattt caattttagg accagcatta acactaattg
    11101 tttttgttcg aacaattcaa actcaattag aatcttcaaa aaacttaatt aaagataacg
    11161 tcagtaaaga tttttataat ttgctaaatt tattcaaaag tatacaggat aaaatgaatt
    11221 ctgaattatt tagtacaatt gaaaaaagaa ttaaagtacc tataaataat ataaatacaa
    11281 atttagatgt tagagaaaat aaagaaacta ctaaagaagt acaggaggat ttaaaagaat
    11341 atagtgcatc attgaataaa gttttaagcg aaagcgttaa aaaaattaat gaaatatttg
    11401 aggataacta caaagacata ggtagttatc taaaagtatt ccataggatt cttaaaatac
    11461 taaataacag gtatgaatca aaagatattt cgcatgaaga atacaaaatg tatgttggca
    11521 tattacgtag tcagattgaa gattacgaat tttatataat tcttataaat tctttgttta
    11581 ctcttcgcgg aacgggtatg gggattgagt taattgaaac atcattcttt ggtgatgaac
    11641 tagatattga tttaaaacag catttcgaac atccagagtt tttaactgaa atcattaagg
    11701 ataattttgt tgaaaagaaa tcgtatcgtg aatcagtaaa aacaagaaaa aaaatagaaa
    11761 actatttgaa aatagaaaga taaatgataa cttattatct cttttgagaa taagatacac
    11821 gttacaagat ggaaataaaa ttattataag taatggagta tagtcctaga aatagggctt
    11881 tttattttac cttgaaaggt ggtgaaaata tgtgagttta tttgatatct ttaagcaatc
    11941 aataaagaac gaagatccat cggattggat tcctgacttt gtgtttggtg atgaagaatc
    12001 tgctagagca tatttgaaaa tcatggctaa aagtacagtt cttgattttg ttgcaagaac
    12061 tatgtcaacg ttagaagtta agtttaaaaa taaagatggg acatcagatt gggattacat
    12121 tctaaatgtc cgacctaatt ctgatatgtc agcagctaca ttttggcaaa aattttttta
    12181 tcggctctta gatgacaacg aagtgttagt gatatttaca gatgataatc aattgctgat
    12241 tgctgatgat tttagtcgaa cagagtatgc cgtgtatgat gatgttttta caaatgtgat
    12301 ggtgaaaaat tttgtctttg aacgttcatg ggccatgtct gacgtcattt atatggaata
    12361 taataacgaa gaattagaaa ggtttacacg aggtttattt gaagattacg ccgagttgtt
    12421 cggacggatt ctagaagttg caatgcgcaa taaccaaatt cgtgggtctg tctccattga
    12481 ttcaacagga acggcaaatg atgaaaaagg aaaagatggt aagactagat ctcaaagatt
    12541 acaagaatat attgataaga tttggaactc ttttaaaact aactcagttg caattgttcc
    12601 caaagtgaaa ggctttgagt atgaggaata caccaataaa caaggcgtat cgaatcaatc
    12661 actagatgaa ctgaataaga tgaagtcgtc tttaattgcc gatgttgcca acgccatagg
    12721 agtacctacg gcgcttattt atggcgaaaa agctgaacta gattcaaata ttaaagcttt
    12781 tcgtaaattg tgtattgctc cactagttaa aaaacttcag gacgaattgg tggcaaaaat
    12841 aattagtcga caagagtaca aaaacgggga acgtatagaa gttaaaaaag ttctccaaaa
    12901 agatctatta gagtatgcta ctcaaattga caaaataaca tctggtggtg cgttttatat
    12961 tgacgaagtt cgtgatgaag ttgattatga tgagttgcca aataatgaag gaaaaattcg
    13021 tattcgtacg aaaaactatg aagaaactgt gaagggaggt gagaacgaca atgccgaaag
    13081 ctaaaaaagt accatttcag tttactaacg aaattcagaa tggtaagcac attctcacct
    13141 tgagtggaaa tgtacagaaa aaatattggc gtgatgatga tgtcataaac gctaaagata
    13201 ttcgtgactc tctagatact gtaaccgatg atattgtaat caagttgaac agcccaggcg
    13261 gtgatgtgtt tgaagggatt gagatttaca attatttaaa agatcaccca tcaaatatta
    13321 ctgtagaagt tacagggctt gccgcaagcg cagcaacgtt tattgtcgct ggtgccaatg
    13381 atgtgattat gaatgttggg acttctttga tgattcatga agcttctaca tttgcttggg
    13441 ggaataaaca ggacatccaa aaaacgttaa acgcattgga aacgatcgat gattctatct
    13501 tagctattta ttcagataag actggccaat ctgttgataa gttgcgtgaa tggatgaatg
    13561 aagaaaaatg gtttacagca gatgaagctg tcaaatatgg atttgccaat tctgtgaagc
    13621 gtgaagaacc acaaaacgaa cctcaaaata ttgcagcgat gattcaagat gctgttgccg
    13681 ttgcaatagc gaatttaaag gaacctgtta ctaataaaat cgaacaagaa tcaaaacaaa
    13741 aatcattaat cgcacgattg cgaaaaggag aataaagata tgttaaaaat tacagataaa
    13801 actgcagatg caaaaaaggc atttaatgct atttcttcga aagaagatgc aacacccgaa
    13861 caaattaatg ctgctctaga agaatatgta actgccattg cagaagatgc cggcaaacag
    13921 gtgcgtgctg aatatgaaga attgaaaaat gtgacggaca atcgtgtatt agaggctcgt
    13981 ggaattccta cactaacagt agaagaaaca aagttttata atgaagccgt aaaatctgga
    14041 ggtttcgatt ctgacgtagt ttggccagaa acgattttgg aacgtgtttt tgaaaatctt
    14101 caaaacgatc atcctatttt aggcctcatt aactttacac caactgtagg tcgtgtcaaa
    14161 gtaattagag cacgtcgtaa aggtgttgct gtttttggac cattgcataa agaccttgaa
    14221 ggccaactag atgctgaatt tggtgcaact gaatttgttc aacttgcgtt gaccgcattt
    14281 ttcttaattt ctaatgatac cttagattta ggaccacgat ggattgaccg ttttatcaat
    14341 ctttctttaa gtgaagcagt acgtgatgtt tgggctaaaa aaattattac aggttctggt
    14401 aaagatgagc caattggatt gcttaaagat ctagatggtg cggtcacaag tggtgtatat
    14461 ccagataaag ctagcgcagg gacattaact tttgcgaaag agaagattgt tcttgaactt
    14521 gcaggggtga tgaaaaaatt atctaaatac acatacaaag tagatggtga cgatgaaggt
    14581 gaagtggaat accgttctgt tgctggacga gtgtatctaa ttgttaatcc tgttgattac
    14641 tgggatatcg tagctagagt tacatttgca aatttaaaca atgtgtatgg gactacgatg
    14701 ccatttatcg ctgttgatca tatcatcgaa tctgtagacg taccagaaag taaatgcatt
    14761 gcatacgttg ataaagagta tgaagctact caatcaagag ctgaaaaagt ctatgtatat
    14821 aaagaaactt ttgcaatgaa acgcgcaact ttgtatgctg tcgacatgct tggtaatggc
    14881 tatccaacaa ataatgatgc agcacaagta tatgacttgg actttacagc gggggagtaa
    14941 cgtcccctgc aattggtgag ataacaccga cgactgatgg ggcagtcatt aatctttcgt
    15001 aaatggggtt agagtatgaa cactgaagaa tttataaaag aatataaggc ccgatttcgt
    15061 attttccata cttccgaaga tgaggatatc gggaaacagt tagagagcgg gtttgccgat
    15121 ataaaatctc taatcggcag ttttgatcct tccacttatg agaaaggaaa agaattagtt
    15181 tatgaacgta ctcggtatct acggaacgaa tcactagagt acttttacga caattttcaa
    15241 acaatgatta tggatgcatc aattgatctg gcaggtgctg gatatgccaa ttaaatcaaa
    15301 ttataagaag cctaaaattg tagctggtga tctaaatacg ccagttactt tttttgagtt
    15361 tcaacctaat aagggtccag aacctggtga tgaagaaaaa aaggaactgt atttttgtac
    15421 agcactagtt tacaacccat caatgaaaga tcgtgacatt cttggcacaa aagaaggcgt
    15481 tacgataaaa atacgggatc cccatcaatc ttatattcct actaacaaac ataaagtaag
    15541 gatattagat tatcgatatg gagataaagt ttgggaaata atcgatgtct caccggattt
    15601 ggaagaaaat tcaattgtaa agatagtatt agaattaatt acttaaattt ggtgataaac
    15661 gatgagtaac gtaaagtttg aaggcgttca agaaacaatc caagctttag aaaaaaagtt
    15721 tggagaaaag aaaactaaga cattaacaaa aaaagcaatc aatgttggcg ctgaaaaagt
    15781 agaaaaacag ttgcaaacgg acatgaccgt ttttaaagac aaaggttata cgattgatga
    15841 ggttgtacgt aagaacgcta cgtatagaaa ttacaaggca gaagctgaaa tcggttggaa
    15901 tggtcctcat cagcgttata gaatcattca cttaaatgaa tggggatata cacgaaaagg
    15961 taagcaaatt aggccaagag gttttggcgt aatcactaaa tcgcttaaaa attccgagcc
    16021 actatatttt gatgctgttg gggaggaggt taaaaagaga ttatgaaaga tatgctcaat
    16081 atcatttatg aggctctttg tttgaatgag tatattcgtt caatgacata taacgagaat
    16141 accgagcaat atcgtattaa attttatgag caacctgaaa ccgccgataa aacagggcca
    16201 atgatcacac ttcgtcctgt agatacacct aacgaagcat atcacggaag taacaaagag
    16261 ctttctgtag agcatatggt acagattgat gttgaatcga aatatagagc aacatgcaag
    16321 cagattcaat atgagataaa aaaagagatg aaaaaactag gctttggcca aatttctggt
    16381 caagggttag atgaatactt ttctgaaaca aaacgctttg tagacgcgcg tcgttacgaa
    16441 ggaaatacaa aaatttacga taccgaatat tagaaattaa gacacgaaaa taacgtgtct
    16501 ttttttatta cccaaaatta ggaggaaaat tttatgactt tagtaggatt caaaaaaatg
    16561 acaatcggga tctttgacag cacagggaag attccagcag caaatttata tgtaattgaa
    16621 ggtgaacaag acaaaggggc gactgtatct gctgaaatca gtgggctatc aaaagaacca
    16681 tcaaaagttt acggatcgaa catcgcttac tatgtctctc aaaaaggaac aggcgatgtt
    16741 tcagctactt ttggattgtt agatttacca acagaagtaa atgacaaaat tcttggttac
    16801 aaaaccgata caaacaaaat cagcttcttg ggagaagata ctgagccacc atattgtgca
    16861 attttgatgg aatcggagga tttgaacggc gatacagcaa tgcttgcgat gttcaaagga
    16921 aaattcagtc gtgaatctat taatcttaac actacgacaa atgaagcttt cgaaccagaa
    16981 gcagaagaat atgtattttc agcgattgcc aatgatgttg aaggtgatgc taaaggccaa
    17041 acagtcgcaa aatatgttgg agacgaagaa gcttctatta ctgctttgaa agagatggta
    17101 tttcctgcag cgggggagta actagctccg ttgttggagc aatcactccc actattaatg
    17161 gggtaacagt tgaattaagt taggaggata actatgccag atacatttaa aatctataaa
    17221 aaaaatggta ctgaatttac aaaagtcgca gagggtgaaa gtccactttc tattactgga
    17281 attgcagcaa atactcaagt tgctaaagga gactaccaag ctacgcgtgt ggttggaggt
    17341 caagaatcag taaaagtaga tattcctgca tttaccactc ttccaatttc agttactggt
    17401 gtgacgttgg ataaaacgac agctgaagtc gaacaaggcg gaaccttaaa attaactcct
    17461 actgtaacac cagcaaatgc aacagacaaa acaggaagct ggagcagttc gaatacagct
    17521 gtagctactg ttactggagg caacgttaca gtaaaaacag acgctgaagt tggcggaact
    17581 acagaagttt catttactac tacggatggc ggtaaagtag caaaatgtac tattacagtt
    17641 attgaaaaag cagcgggcta gtcttcggac tagctttttt atttggaaaa taatggagga
    17701 atctaaatgg caaaagtaag aattgaattg aaagaagaat caggaaagaa agtctatgaa
    17761 aacatagaca caacaggtaa agattatcga aaagctttag aaacaatcaa aaagctaaat
    17821 gaagatggag taatgatttg gaatcgttta gacatttatt tggattttgc tgtaggtatc
    17881 ttcagggata gcaaattgac agctgaacaa attcttgatg gcttaccatc tgagaaggta
    17941 atgagcacat tggacgacat cctaggagaa gtaatgggga ttgaaagtaa tccagaccca
    18001 gacgcaaaaa agtaacaccc gaagaagcag aagaaatgta cttaaaccta tgcagagagt
    18061 tagtgaaagc gggatggtca ctatctgaca tagaagataa ttcctttgaa atgatgatga
    18121 aaattgcgtg cacttcgcct aagaaagaga aacaaaaaga agttgatctt aaagacttct
    18181 tgaaatccat gtagagagga ggtaaacata tggcaaatgg aaaaccttta ggcaatatga
    18241 ttataaaact agacttagac agttcagctt tttcaaaagg tctagccgga gctaaaaacg
    18301 ctgttaacca tcaaatgaaa gcaatgaaat cacagatgca agtaatgaac tcttcaggga
    18361 attctctcgg tgctttacaa gcaaaatata atggtttagg cggagttcta caagcaaatg
    18421 aaaagcaagt agaattgctc actaaggctt ataaggatag ttttgactct aatggaaacg
    18481 ctactgcttc gactgctaaa tacgctaatc aattgaatca agcaacagct agatcagcaa
    18541 gttacgaatc tcagatgaaa acaaccgttg gacagattgc tcggatgaaa gtggaaacgg
    18601 aagggattac ggggaaacta aaagctaatt ctgatgtatt gattaattct gggaaaaaaa
    18661 ttgaagcctt tggtaaagga gtttctggga taggatctgc cctaactgtt ggagtaacag
    18721 ctccaatagt ggcaggtgca actgctgtta ctagtgcagc tatttcttgg gaaagtgctt
    18781 ttgcaggagt aaaaaagaca aatgatgaag ttgtagattc gaatgggaac gtagtttact
    18841 cctacagtga tttagaaaaa ggccttcgtg atttagcgac taaattgcca gcaagccacc
    18901 aagaaatagc gaacgttgct gaagcagcag gtcaattagg gattaagaca gaaaatgtag
    18961 ttagtttcac caagacaatg attgatatgg gcgaatctac taatatgtca gcagaaacag
    19021 ctgcaacttc tttagcacga tttgctaata taacccaaat gtctcaaaaa gactttgata
    19081 aattaggatc agtcattgtt gatctaggta acaattttgc tacaaccgaa tcggaaatca
    19141 ctgaaatggg actgcggtta gcgggtgctg gtaaacagat tggaatgagc caaggcgaaa
    19201 ttctaggatt tgctactgct ctatcctctg ttggtgtcga agctgaagca ggtgggtctg
    19261 ccttttcgaa ggtcatgatc cagatgcaac tagctgtaga aaaaggattt ggcgcttttg
    19321 atcagctgaa acagatggca gaagaacaag gtgttccgtg ggttaattta gtaaatgctg
    19381 tgagagacgg tggaaaatcg ttaaaagcag tatcagagca aatgggattc acttcatctg
    19441 atttaaagaa aatgtataaa gaagcggaca attcgaaaag ttcgcttgaa aattttgcga
    19501 atgtggcagg tgttacaagt gaacaattcc agaagttgtt caagagtgat ccttcagaag
    19561 caatcatcaa gttcattcaa ggactgaaag attcagaaaa acatgggact tcagcaatca
    19621 aagttctgga tgacatggat ataaaagaag ttcgactacg cgacagttta ttgagagctg
    19681 caaatgctag tgacatattc aaaggagcaa ttgaaaaagg aaacggcgcg tggaaacaaa
    19741 atacagcttt aacaaatgaa gctaataaaa gatacgaaac aactgaatca aagctaaaga
    19801 tgttgaaaaa cgaagtgatt gatacagcaa tcgatcttgg aggtccattt gtagacgcgt
    19861 tgcgtgatgg tctaaatgta tcaaaaccgt tgataaaaaa cttagggaat atggcgaaag
    19921 ctttctcagc catgagtgat gatcaacaaa gaaacattat caaatgggtt ggattagcag
    19981 cagcagctgg tccagcgctg aagcttttag gaactggtac ttcagtaata ggtaaagtta
    20041 cttcaactac tggaagattg acgaaaagcc ttgttgattt agcagcgaaa gcagcagaaa
    20101 agaaggctct ggatgccttt gcatcgtcgg taacaacagc tggtgctgca gctgcaaaga
    20161 cagcaggagc tggaggtatt ggcgggttag tccctatgat gggaaaaaca gctagtgctg
    20221 caagtactgc tgcgggcgct agcggaatag gcgcaatgac aacatcacta ggtttgttag
    20281 gtcctgctct acttggtatt gttggcgttg gtggcgcact agcagttggc tatggagcat
    20341 ggaaattatt tggtgaagaa gcttggaatt cctcgcaaag agtaaagcaa tggggaacag
    20401 atgtcggtga ggaaatagat aatactcttg acggagtaca gaagaatatt gaaggtgcaa
    20461 atggtcaatt cggtcttctc aaagagggtt ttactgttga tgatgcgaat aatatggcgg
    20521 ctaatttcgc aaccgctgga acatctttgg aaacatcttt aacaggtaga ataaaggcat
    20581 tagacgatgc actaaaaggg ctacctcaat cagttcagga tgccatgaaa gaagtagctg
    20641 aatcagaaga agaaacctta gggcaatctc tttctaaaat aaaagacaac aatgaagaaa
    20701 taaaaaaaat cagagaaaga gccgcaaatc agaacagaga cttgactgtt tcagaactac
    20761 aacagataca ggatctgatg aaaggaactt ctcaagcgta tgtggatacg ctcgatgttt
    20821 ctgctaaaga cagaaaaaag atactatcag ccatgaatgg tgatgttgca caggctacag
    20881 aagaagaagc gaagacgtgg ttacaatctt tagcgaaaca aaaagctgca actaccgaaa
    20941 attatagaca aatgcttgaa gaaaaaaagg cggctttaaa ggaaaaggga gtaagcaaag
    21001 aattcattga tgcattagaa gatgaaatga atgattacat caaaactaca aatcaaggat
    21061 tcgatagcca aatagctgct ataacaaata aatatccaca attgattaac gaagtgagtt
    21121 tatcaaatgg tcaattattt agcaaaacgg cagctgctat ggacaatact ggtcagtata
    21181 caaaagtttt tctcgaaagt aatgaaaaga ttttagccca agcgaagaaa acatcggatg
    21241 aaatagcaga taatgccgaa aaaaatgcag aacaacttgg tatggtagct gatgaggcta
    21301 cgcatgctgg atcaacttgg aatggattag ttttagacga aaaaacagga aaagtaaaaa
    21361 ctaacgtaca agaagtagtg aatgaagcaa cgaaggattt cactaaatgg aatgaactta
    21421 aacctgttgt tcatgatgcg aaactaacaa cgaacgcaaa agatgtcatt tccatagcgg
    21481 cagtacaaaa tggttattgg gatgatatgg aatggaaaga aaaagaagca ttattagaag
    21541 atgagtgttc taaaaatgta tacaaggctt tagaaaatgc tggtaaatgg aatgagttag
    21601 atattccttc taaagaagca atcctaactt caaatactcc taaagttatg ggtgaaactc
    21661 tcatgaattt gggactatgg gatacttaca aacttcaagt gaaagattta gatttgaatg
    21721 cgtataaagt atacgatgct atcgatggat cagaagaaaa attaaatagt tgggctcaat
    21781 tagataaccc cacaaaagaa ttgttattag ataactcaga tttttctaca aaacttttta
    21841 attccgaaga aatgtttaaa cgttggaatc agttatcatc taatgcaaag tttctttcag
    21901 ctaataatac taaatttaca gaggttcttt tatcatctga cagaatgtgg aatcaatggg
    21961 taaaccttcc tgataatgag aaaagattac ttgcagataa cgaagattta gctaccactg
    22021 ttttatcttc agaagaaata tacggaagat ggactcaatt accagataat caaaaatata
    22081 tgttagctga caatactgac ttagcaaaca aaatatttgc ttcagaagaa tcatggaatg
    22141 catggaaagc aattccagac atagttaaaa acttgcttgg taataatatt gatttactgg
    22201 ctaaagtgaa agatgggact gtaagtgtag aagaatataa caaagttatg ccacttctta
    22261 aaactctata tggagataac gttaatttaa aagaggcaat cagtggggct aaaagctcta
    22321 tagagaatta caacaaaaat atttttccta atgataaaac tgcagtagga cacgatgagg
    22381 cttcacaagc tgctaaagat gcatttgaag cgttcaacat cttccaaaca aaaataccag
    22441 ataaaattac taaaactgta tcagctgact ttataggtcc tatgcctaat gctaaaggaa
    22501 cgaacttcca tcctggcgga gcagctatgg taaacgatca aaaaggacct acttatgaag
    22561 agcttataac cttgccaagc ggagaagctt ttatacctaa aggacgtaat gtcgttttag
    22621 acttaccaag aggttcgaaa gtactgaacg ccactaaaac taaacgtcta gtacctaaat
    22681 atgctgatgg tataggaaac ataacgacgg tttcatctac gctaaactta gatgctttaa
    22741 ttttagctat taacgagcta acgacggtat tgagaactca actgatggca gaaacgacta
    22801 atgctaaaga tactaaacta tcagatgaaa caaaagttgc cgatccgatt attccgggca
    22861 gtctttcaga aaaatcagat cagtatctag gaattggagc tcaatggtta accaatctaa
    22921 tgaatggttg gaactcagcg gttcctcaat atatgaatag tgaaatggtt tttattacaa
    22981 actatctcaa tgcgctaaaa cttcaaaaca atcctaatta tacgcaagga gtagcatgga
    23041 ataagaactt actgaatgga tggaatagtt taactgggac atttatttct acgatcaatt
    23101 cattttgtaa tcaagcaatg gtcacgctta gaaactacaa cactcctatg tacaacaatg
    23161 gccgaacgtg gcagcaaaat aatcttaatg gttggaattc attgtatgga tcattcatag
    23221 cccgtgtaaa tcagcttggt aacgattcga ttaacaacct tcgttctaaa agtggtggtt
    23281 tttacaatgc agggacattc ttgttgcaat ctttaataaa cggaatgaat tcgttaggta
    23341 attctctttc tacaacaatg aacaatgtcg ctaatacaat ggttggtggt atgggcaaag
    23401 gggttaatgg cgtcatatcg ggagttaact atgtgcttaa ggaagtcgag tcaagtaaga
    23461 atatcggaaa ctggccaatt ccacaatacg ctaagggaac tgatggacat cctggtggac
    23521 ttgctatgat taatgatcaa aaaggcctag ttcacgaaga atatgtacag atgccagatg
    23581 gacgtggttt tattgccaaa ggtagagacc ttcttgttaa tttgcctaaa ggtgctcagg
    23641 tcttaaatgc ttctttaaca aagaaattaa aggaacgttt aaatattcca cactacgaaa
    23701 aaggtattgg caatttagat attgttgatt tgttagatga tgaaaaaaga atgttagaat
    23761 tcttgactag caaagttgac ttttcaggca ttaatgaacc atggcttgat atgactaaat
    23821 caggaacgtc attaatgtct aaagctgcaa atacaatgct tcaatcaaaa ttaagtgaat
    23881 tcttcactca tggcaatttt gatggtgcag taaatgccaa tggtgtttat caatatttag
    23941 ttgatgttgc acagaaagta atgggtaagt tccccggact tacagtaact tctggatatc
    24001 gagcaggaga tgcttactat cacggaaaac ggcaagctat agacttggct tatccaggta
    24061 tatctggaga tccgagatat acagcagcag ctaattacgc ttttgaaaaa ttcccttcaa
    24121 aaattgcgta tgtcattacg aatggacgtg tacgtgaccg catgggatta tctggaacag
    24181 ggtcaagcgg acaatggacg aattggccag atggcgatca cttcgatcat attcatttaa
    24241 acggttcgat ggggtctggc gatatattca atggaggagt tggaggaagt ggtgttgcac
    24301 gctggcgttc ttatgtgtct aaagcattga aaatgaatgg attaccagct acagcagctt
    24361 atataaatgc ctggatgtcc caaatccaaa cggaatctgg tggtaatgag aaagcaatcg
    24421 gcggcaatga tgggctcgct gaaggcaatg caacaggatt acttcaaacg aaacccggaa
    24481 cttttgcagc taacgctttt cctggtcacg gaaatattat gaacggattt gataacatgt
    24541 tagcagcaat caattatgct aagaaacgtt acggagttgc aggtatgttg caagttatcg
    24601 ggaaaggaca tggatatgcc aatggtggat tgattaccaa agacggacta tatcgagcgg
    24661 gagaaggaaa taaaccagaa atggttattc cattgacaag aaaaacaaga gctattgaat
    24721 tgatgggtca agcattagct tttctttctg gcgatgacaa gaaacgctcc aacactacaa
    24781 atacagctga taattcagca gagcttgtaa ctttgattaa acagcaacaa aaacagcata
    24841 atgagttaat gatgattctt agagcgattc ttggaaaaga tttaagtctc aagtcttctg
    24901 acattgggca agctgctaat cattatatgg gctctgattt gaataaactt cgatatacga
    24961 atggaggtgt ttgataattg ttttacaaat tacagttcaa tcaaaatggg aagttgtttg
    25021 atccgcaaag aaaagaaaaa attgtatgta aagaaatcaa acgtcaagca ccagtgtatg
    25081 aagtgaatta cgaagatttt gaagggacga acggtagcag agaagctaat gctagttttc
    25141 gtccttttga attagtgctg acttttgata tcttttacaa aaataagcat gataaagaac
    25201 tgctattaac agagttctat gagctaattt ttgtcggtta tcaatattac atttcctatg
    25261 atttaagccc aggaaaaaga tttaaagtaa atccaacaaa tttcgaaatt actgaggaag
    25321 agaacgatta ttctacgata gaaataacct ttagtattcc atcagggagt gctgaatcca
    25381 gcgcaaccac tttaacggat ttcaatctag aggaagaatg gcaattctcg caaggtctag
    25441 tggcggaaga ttacaagtat acgcatcaga ctagtcactt cattatttac aatgctggaa
    25501 gctttgaaat tgatccacgt gaacattatc tacggatcgc attagaagga gaatcagaag
    25561 gaaatgtgac gattttcaat aaaacaactg gcgatcgatt tatttactat ccatcacttt
    25621 ctacgaatct cggacagaca ttagtcttgg atggcgtgat tccaaaattg aacggtgtaa
    25681 gttgtggaat caatacgaat cacggtctga tcaatttggt tgagggagtc aatgaaatcg
    25741 agatccaaaa tattactcgc gtgaaatctt catgggattt ccgtttctta tataagtagg
    25801 tgattgagtg actgagttaa ttattcgaaa ttatgaacaa accaaagaag aaatccttgt
    25861 cggttatgac aagggttctt tttatgaaaa ctggcaacaa aatgaaacgt gggagattgg
    25921 ctttaccgtt accagcaatt cgttgaatca agaagtattt gatttagtcg aatacgagtc
    25981 ttctgttttc tacaacggac aggaatttgt gatcaaagaa atgactcgca aagcacttgg
    26041 acaattgctg acgaaacaag tagttgcgac acatatctat tacaccgttc aagatggtta
    26101 tcagtacaac acagtgacag gtgcgaaatc tattagccag ttactcacgc atgtatttag
    26161 tgcagggagt cgcggcttta catgggaagt cattgatcca aacaaaaaat tccttaccgt
    26221 tgaacaagaa aatttcggta acgcgaatta cttgaagctg atcaatgaga ttctatctga
    26281 ctataatgca gtcgtgattc cgaataataa acatctaact ttctatcctg ccagtgagta
    26341 cggccagcag acggaagaac agattcgcta taaatacaat acagatgaag tgtcgtttga
    26401 tattgatacg tattccttga agacgcagat caaaggctat ggaaaattga aggatggtgc
    26461 aaatactgag aatcctaaag atagtgacta tatatttact cccatcactt atacaagccc
    26521 tgaatcacag aagtggggaa tcaggataca agatcctgtt aaagacgaac ggtataccgt
    26581 atcagggaac atgctcgagc ggttaaagac agacttgcaa gactatccaa atatttcggg
    26641 atccgtaacc ttgaaatgga aaatcagtcc caacaaaggc gatcacgtcc cattcattta
    26701 tgaacctttg aatattaata cgtacattca agtggtagga atcaagacgt atccagcgat
    26761 accaaataag ccaccagaaa tcacattgag caacacaaag aaaacaatga cgtcgatact
    26821 cgctgaaatg gcgaagaaag gagtgatttg atggggttat taaaattaat cagtaaccgt
    26881 atctctacgg aatggaaaga gaaatttaat aaaaacattg actaccttaa tgatcttgaa
    26941 aagaaactgt ctgatcaaga caaatcaatg aacagtcgta ttgataatct cgtgcttcat
    27001 tcaggtggcg aatcgccgaa tgaagtggtt gatgctcgtg taaataataa gggagaaacc
    27061 tttcctactt tacacggcag attggtagaa cacgaaaccc tgacagatga acaaattagc
    27121 gaactgaata caaacatgga tagtcaaaaa gagcaagttc aacaattgaa caagtctgtt
    27181 caacaaatta ttggtggata caatgagcca attaacatct atgtttctaa agatggtagt
    27241 gatattttgg gtgatggatc agaagaaaag cccttcttaa ccattcaaac agccgtaaat
    27301 aatgttcctc taattacaac gtctcaaatt actatttggg taggtagtgg ggcttactta
    27361 gaagatatta tgattagaaa tttaaatttt acatcatttt taattcgacc tatcgataat
    27421 tttgacacta ttgatccatt aaaatcagat ttacctgtta aaatacgatc catttgtttc
    27481 actgcttgca aaggttattg tcaagttgct ggaatgcaaa ttgtggatac agcaaacgga
    27541 gcagactatg gaatcagaaa tgagcaaagt ggctatatgg caatcaatag atgtaaattc
    27601 gcagagaaca caaaaacact tgcaagatat aatgcagttt atgtcggcgg aacatcaaag
    27661 attaatatgt acggagacac gacgttgatt aaccagaaag tggcaattta cgctgtattg
    27721 atgggtgaaa tttttgtcag tgcgtttggt tcaggaaacg acgtaggaat tttgtgcgag
    27781 aatggcactg tacgaggaac tgtatcaact tcttttgcca caaccccaac aaaaactact
    27841 gggtacggtc tcatcatcac gaaaggcacg gtgttgcaat aatggtatac aaaacaaatg
    27901 aatcgatcat catgattcaa gcagaagcca ctagtccaaa caggacgaat gttgtttttt
    27961 ggtcgcatga tcgaggaaca gctaagcttc gaatgaaatt agttcggaaa aacggcattc
    28021 ctcaaagctt acccgaaggc acaactgttc cgattcgctt gatgctcaaa tctgcaacgg
    28081 cagaaggtgg ttatggaaaa catgactatc tcgccaccat tgaagatcgt gtaaatggta
    28141 ttgtgtccat tgtgctggaa gataatattt taggctatgt tggaattgtc gaaggtagtg
    28201 tttatattga tttcccaaac gaccgctcgt tagatacagc tggccgtttt acttttgaca
    28261 tcaaacgcag tccaatcgat gatagtacac cagaattgga agattattat ttcaatggtt
    28321 tcagtcagac tattgataaa atcgaaaaaa ttctagctga tggaaagcaa gagattgaac
    28381 agaaaattac ggaatctgaa acgcagattg atgggaaact gaaagaaaca agcaacaaaa
    28441 tcacgaaagc caatcaagat gtcgcaacta tcaatacgaa tattgataaa gcaaatgatc
    28501 gtattgatca aacgaaccag caaataagtg atctcggaaa gctgaaaaag atgtactcca
    28561 acagcatcga tttcgggaac tatgattata gtggaaatcc gaatttgatg gctaatataa
    28621 acgctgatag cttctcgcaa ggtagtggtg ccttatctgt tgtagatgat ggtgacgagg
    28681 tagtgattac gcttgatcca aaccataaat tagaaatatt caaagcgaaa agtcaaccag
    28741 ctctggtagt ggggaaaaca tataccatga gtgtagaaat tatgttagaa gatgatttca
    28801 ctggagaccc tagtaatata tctctaagat atattaaaat gcccagctgg ttaacagtac
    28861 tatatacgcc ttatacatta actaatacta agggtgtatg gcaaaaacta actgttaccg
    28921 ctaaaatgac tactgcgatt gataacgctg aaagctggtt tataatgcta caaaataaag
    28981 acgctaataa cagcctatcc ggcaaactac gtttgagaca cgctaaactc gaagaaggct
    29041 caacagctac accattccaa cctaacttat tagcagaacc ttacaacatg tgtcgcgaat
    29101 atcctaacga aaatattgcc gatcctaaag ttaagttccc aatcgaatct ggcgaccacc
    29161 aaatatatca aggctacaca gaagaagagc ttatgatagg tcaaacgtat actatcacgc
    29221 ttaaaggaac aaaacccgca agtcaaacct ttgtagcgta taatcattgg actgctcgtt
    29281 taggagaact aaagccggtt gatgggttga cagacgtatg gtctctaaca ttcacaccaa
    29341 cgaatgttgt ggcgatgcct aaacttttcc gtgtttatca gtatccacga tcaacagtag
    29401 gcgcatgcca aattgactgg ctcaagattg aaaaaggcaa cacccgaacg cccaatatta
    29461 gtcagtttaa atacttcggt gaaggattga aagacagcaa caatccgaat gattacagtt
    29521 gggatgtcac acctgaatat actgaaaaag gcttgaataa tgctgttaat gtgtatgatc
    29581 ctcagagagt tgaaggtttg aaaaactttg ccgatggtat tcaaatagca ggagataaag
    29641 tgatcagtga aaatgattgc actgtttata cattaactaa agacaacagt caatcgttta
    29701 tcgatgggta tgcaacattt attaaacacg gaaaagaagt tgttgtaaat ggtacagtaa
    29761 agttcaaaaa agcttatgct tttggtgtac cacttgatga tgaagtccca gatgaattta
    29821 ttgcaagaat tgttcatggc atgcttacag gtcagtcagg aacaaatagt gtatcaaaag
    29881 cgatgtacgt gcaaaaagac ttaggaaaaa ttatgacgaa tagcgaattt gccgcaaatg
    29941 aatggtttac attccacggt aattattggg taggagtgaa ataaatgaaa aacatttgga
    30001 aatacggacg tactggtgga gagtacgcag gaaaagtatt ggacgacatg cttgtatccg
    30061 ttccttacac ggatcagcca ccgctcgaag gattacgtgc tgatggcgaa ccgctaacga
    30121 ttgctgatca gatgtttgat cctaaactga accaatggat tgttttagcg aacacactgg
    30181 atcacaacga tttaaacaat ctcaaagcga tgtacgaggc actggaacac gagaacggcg
    30241 atttaaaaca gctcaacgcc aaactcatgc taagcgatgt agcaattaaa caggaaaata
    30301 ctgcattgaa agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt
    30361 cgttacaaaa tagcaaagac atttcagaaa ttaaagagca actaaatcca actttaaagg
    30421 gaggtgagta gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttt
    30481 actgacgatc aagttcgact attcgttcca ctatgcatta cagacgaaga agcagataaa
    30541 atcattagca aagaagagag cgcatcttaa gtgatgcgtt tttttgttgg aaagttggtg
    30601 gaacatgaaa gaagaagcgc tccaagacgt tgtggagaga ttagtaagaa ttgaaacaaa
    30661 attagacaac tacgaatcac ttagagaaaa ggctgatagt gcaaaagatt tagcagataa
    30721 agcctattca gtagcactaa acaatgcaga agacatcaag gaaatgaaga acaataataa
    30781 atgggcttgg ggctatatga ttggcttagg cattacgatc attggctatt tcttgactaa
    30841 attgtaaagg aggtgagaag aaatgatttt accagataag tattatcaag tcattaaatg
    30901 gacagtttta acagtattgc cagctgcatc tgctttagta gccatgttag ggaaagcgta
    30961 tggatggaat ggaacagata tgatagtgtt gactatcaat gcagtagcaa catttttagg
    31021 cgttatcact ggtgtgtcgg cttataattt gaaaaaatag gaggaaacaa atgaaaaaga
    31081 aaattactat tactgcgatg agcctattaa cggctctttt tttattgcca attaatgggt
    31141 ttgcctatac tatcaacaat gaatttaatt tgggcgcaaa tgaaggtagc tcacaaatag
    31201 caaataatca gtacatttta ctgcatgaaa cggctaatga aacagcaaca ggacgcaatg
    31261 aagcgcagta tatgcaacgt tcatggacta gcgcttatac tgcttatatt gtgggagacg
    31321 gcggaattgt ttatcaagtc ggtcaacctg gttatgtaca gtacggtgct ggttcgtatg
    31381 ctaatgccaa cagtcctgtg cagattgagt tacaacacac acatgataaa gcaacgtttg
    31441 agaaaaacta caaggcatac gttgaattgg ctagagattc agcaatgaaa tatggtattc
    31501 cattaacatt agacacgcct tataaccaac caggaatcaa atcacattta tgggtaacac
    31561 aaaatatctg gggagatcat actgatccat acggttatct ttctgaaatg ggcgtaagta
    31621 aagaaaaatt agcatatgat ttagctcatg gatttaccga tgaaaatcca acaacttctg
    31681 aaaacaagcc tgtcattgat ccaacacgag ctggtgcagc taatcctaca ctgacagatg
    31741 gaacgaatta cgcccacatt gatcagtttg gagaaatcga aaatgcaaat ttgcatgtag
    31801 ctggatggca cattgctaat tataaatacg agtatatttt cattatggac tacaatactg
    31861 gaaaagaact agctagagta agagctgatg ggatttatag accagacgta aatcaagctt
    31921 ataatacttt aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata
    31981 agaaagtata cgtcatgatg cgggcaacga atgatccaga gggaaacact aaaggcggtg
    32041 cgcaagattt ccatgacaaa cgttggtatt taaatattcc taaacgataa aaatagctcc
    32101 tcgttgagga gcattacata actatattga caactataaa aattattcga taaaatagtg
    32161 atgttatcgc atatcttcac tatcacccat aaatagtcac actccaagct atgcgataac
    32221 aggtttgttg ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag
    32281 tacccttagc tcagttggtc agagcagacg gctcataacc gtccggtcgt aggttcgagt
    32341 cctacagggt acattaacgt agccatttga atcgttctgt gttagaattt ttttgaagag
    32401 tattatacaa gctaaagctt ttcttcattg ccactcaaat gagtggcttt tttatgtatc
    32461 cttttatgga ttaatgaaag gatgtttcac atagttatac ttctgtatat ttgaaaagtt
    32521 ttactttgat ttttaaatag aaagacattt gggttaaa
//
LOCUS       DAZKCY010000021        32271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000021 DAZKCY010000000
VERSION     DAZKCY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.021"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            114..2723
                     /gene="clpB"
                     /locus_tag="ACG8XC_000890"
     CDS             114..2723
                     /gene="clpB"
                     /locus_tag="ACG8XC_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP9190606.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2874..3560)
                     /locus_tag="ACG8XC_000891"
     CDS             complement(2874..3560)
                     /locus_tag="ACG8XC_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190607.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3538..4032)
                     /locus_tag="ACG8XC_000892"
     CDS             complement(3538..4032)
                     /locus_tag="ACG8XC_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP9190608.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4052..5272)
                     /locus_tag="ACG8XC_000893"
     CDS             complement(4052..5272)
                     /locus_tag="ACG8XC_000893"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9190609.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5419..7254
                     /gene="lepA"
                     /locus_tag="ACG8XC_000894"
     CDS             5419..7254
                     /gene="lepA"
                     /locus_tag="ACG8XC_000894"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP9190610.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7450..8361
                     /locus_tag="ACG8XC_000895"
     CDS             7450..8361
                     /locus_tag="ACG8XC_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9190611.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            9372..9608
                     /locus_tag="ACG8XC_000896"
     CDS             9372..9608
                     /locus_tag="ACG8XC_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190612.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            9721..9963
                     /locus_tag="ACG8XC_000897"
                     /pseudo
     CDS             9721..9963
                     /locus_tag="ACG8XC_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10053..10292
                     /locus_tag="ACG8XC_000898"
     CDS             10053..10292
                     /locus_tag="ACG8XC_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190613.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            10439..14722
                     /locus_tag="ACG8XC_000899"
     CDS             10439..14722
                     /locus_tag="ACG8XC_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFP9190614.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVISVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            14821..15186
                     /locus_tag="ACG8XC_000900"
     CDS             14821..15186
                     /locus_tag="ACG8XC_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190615.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            15188..15640
                     /locus_tag="ACG8XC_000901"
     CDS             15188..15640
                     /locus_tag="ACG8XC_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190616.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            16089..17564
                     /locus_tag="ACG8XC_000902"
     CDS             16089..17564
                     /locus_tag="ACG8XC_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFP9190617.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            17587..17898
                     /locus_tag="ACG8XC_000903"
     CDS             17587..17898
                     /locus_tag="ACG8XC_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190618.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            18259..19515
                     /locus_tag="ACG8XC_000904"
     CDS             18259..19515
                     /locus_tag="ACG8XC_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9190619.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWARLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            19512..20057
                     /locus_tag="ACG8XC_000905"
     CDS             19512..20057
                     /locus_tag="ACG8XC_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190620.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            20058..20558
                     /locus_tag="ACG8XC_000906"
     CDS             20058..20558
                     /locus_tag="ACG8XC_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190621.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            20917..21195
                     /locus_tag="ACG8XC_000907"
     CDS             20917..21195
                     /locus_tag="ACG8XC_000907"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190622.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            21215..21559
                     /locus_tag="ACG8XC_000908"
     CDS             21215..21559
                     /locus_tag="ACG8XC_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190623.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            21578..22489
                     /locus_tag="ACG8XC_000909"
     CDS             21578..22489
                     /locus_tag="ACG8XC_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP9190624.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            22568..23560
                     /locus_tag="ACG8XC_000910"
     CDS             22568..23560
                     /locus_tag="ACG8XC_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9190625.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            23557..23775
                     /locus_tag="ACG8XC_000911"
     CDS             23557..23775
                     /locus_tag="ACG8XC_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFP9190626.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            23788..24198
                     /locus_tag="ACG8XC_000912"
     CDS             23788..24198
                     /locus_tag="ACG8XC_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFP9190627.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            24250..26778
                     /locus_tag="ACG8XC_000913"
     CDS             24250..26778
                     /locus_tag="ACG8XC_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9190628.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            26794..29004
                     /locus_tag="ACG8XC_000914"
     CDS             26794..29004
                     /locus_tag="ACG8XC_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP9190629.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            29012..30103
                     /locus_tag="ACG8XC_000915"
     CDS             29012..30103
                     /locus_tag="ACG8XC_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9190630.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            30124..30666
                     /locus_tag="ACG8XC_000916"
     CDS             30124..30666
                     /locus_tag="ACG8XC_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFP9190631.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            30861..31118
                     /locus_tag="ACG8XC_000917"
     CDS             30861..31118
                     /locus_tag="ACG8XC_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190632.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            31132..31464
                     /locus_tag="ACG8XC_000918"
     CDS             31132..31464
                     /locus_tag="ACG8XC_000918"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190633.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            31509..32057
                     /locus_tag="ACG8XC_000919"
     CDS             31509..32057
                     /locus_tag="ACG8XC_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFP9190634.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            32064..>32271
                     /locus_tag="ACG8XC_000920"
     CDS             32064..>32271
                     /locus_tag="ACG8XC_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190635.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNE"
BASE COUNT        11031 a         4997 c         6860 g         9383 t
ORIGIN      
        1 aagtcaaaaa actatgtgta tattttgacc aaaattgacc aaagcgatat aatagttttt
       61 gtaaagatca aagaaataag tgatcttacc tttaattagg agggtatacc tgtatgaata
      121 ttgagaaaat gactacgacg cttcaagaag cgattgctga agctcaacag attgcggtca
      181 ctcgaaaaca tcaagatatc gacattgccc atgtatggaa aatcttttta caatctaatc
      241 attttggtcg aaatttctat acggatgccg gattggatgt cgaatctttt gaacatgaaa
      301 ttgatcgtct actggatgag tatccagttg tttctggtgg aaacgtccaa tatggacaaa
      361 acctcagcca gaatttcttt cgattattga atgaagcaga ccaaatacgt gaaagcttcg
      421 gcgacgagtt tctttcaacg gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc
      481 cgttaactgt ttatttgaat aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg
      541 aagagatgag aggaggagaa agagtgacct cacaaaacca agaagaacaa tacaaagcat
      601 tagaaaaata cggcgttgat ttagtacagc aagtcaaaag cggaaaaatg gatccaatca
      661 tcggtcgtga tgaagaaatc cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata
      721 atcctgttct gataggagaa cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc
      781 aacgtatcgt acgcaaagat gtgcctgaaa atttaaaaga taaaacgatt ttttcattag
      841 atatgggtgc attgatcgct ggagcaaaat tccgtggtga atttgaagaa agactaaaag
      901 cagtcttgaa agaagtcaaa aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc
      961 acaatatcgt aggggcagga aaaacagaag gaagtatgga tgccggaaat ctgttgaaac
     1021 caatgcttgc tcgcggtgaa ttacatttga tcggtgccac aactttagat gagtaccgtc
     1081 aatatatgga aaaagacaaa gcgttggaac gtcgctttca aaaggtatta gtcaaagaac
     1141 caacagtgga agatacgatc agtattctgc gtggattaaa agaacgtttt gagatccacc
     1201 atggtgttaa catccatgac aacgcattag tagcagcagc tactttatct gaccgttata
     1261 ttactgatcg gttcttaccg gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga
     1321 ttcgggtaga aatgaactct atgccaacgg aattagatca agttactaga cgtttgatgc
     1381 agttggaaat cgaagaagct gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct
     1441 tgaaaaattt gcaagaagaa ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc
     1501 agtgggaaac agaaaaagaa gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata
     1561 aagcaaaaca cgaattagaa gatgcagaaa ataactatga tttagaacgc gctgctgtat
     1621 tgcgtcatgg tacgatacct caattagaaa aagaattgaa ggaattagaa gctaaagcaa
     1681 aagacagcga gattaagatg gttcaagagt ccgttaccga aaatgagatt gctcaagtcg
     1741 ttggcagatt aacgggtatc cccgtaacga aacttgtaga aggtgaacga gaaaaactaa
     1801 ttaaattgaa tgaaacactt cataaacgag tgattggtca agatgaagca gttgatgcgg
     1861 tcagtgacgc ggtaattcgt tcacgggctg gattgcaaga cccaaatcgt cctctaggtt
     1921 ctttcctttt cctaggacca actggtgtcg gaaaaaccga attagcgaaa gcgttagctg
     1981 agaacttgtt tgactcagaa gatcatatgg tacggatcga tatgagtgaa tatatggaaa
     2041 aacatgctgt gtctcgtcta gtcggagctc ctccaggcta tgtaggttac gaagaaggtg
     2101 gtcaattaac ggaagctgtc agacgaaatc cttatacaat cgtcttgttg gatgaaatcg
     2161 aaaaagcgca tcccgatgtc ttcaatatct tgttacaagt cctagatgac ggacgcttga
     2221 ctgattcaaa agggcgagta gtagatttca aaaatacggt tctgatcatg accagcaata
     2281 tcggttcaca gttactgctg gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg
     2341 ctgaacaagt caacacgcta ttacgtggaa acttcaaacc agaattcttg aaccgaatcg
     2401 acgacacgat tttatttaca ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa
     2461 tggtggcgca attggcacaa cgtctggaac atcaagaaat cctgctgaca atcagtgatg
     2521 aagccaaaac gtggattgct gaaaatgctt atgaaccagc atatggtgca agaccattga
     2581 aacgatttat cactaaggaa gtagaaacac cactagctaa agaaatcgtt gcaggacatg
     2641 ttatgcctaa atcaaaagta acgattactt tacttgatgg acaacttcat ttcaaaacag
     2701 aagagctaga agaaatcgtc taatcttcca ttgagactat aaaagaaaaa aggggtctga
     2761 cacaactagt gtcagacctc cttttttcaa ataaacagtg ttccagaagt agtttcttcg
     2821 aaaaataagc cgaaatttct tgaagctgcc cgcttctgtc acaacctctt ttattaggta
     2881 tttttcactt tttctaattt ataactttca aagtaacgct catctttttt actttgagac
     2941 catgcaaaca atcccattaa ggcagcacct atcgtatttg tcagcaagtc gcccatcgta
     3001 tccatcaatg ctgctcgtcc gacaaacagt gtgccatcag aagcagcaaa acgttgaaga
     3061 ttcatgccta agaactggtc gcataagaat tcccagaact cccaaaacac gccgcaaagt
     3121 ccagcaaacg caaagccgaa taagaggaac aaccaaggac tggtttttga tatctcagca
     3181 tctttcatca agtaaccaat caatccatag ccaagagctg tcaggaccat tggactcacc
     3241 gcatgtaaga ttttgtccca aaaagagatg atactgatca aatgcatccc tgtgcctaaa
     3301 aagacagaga tgaacaagaa aaaccagtaa aacaaaacga ttgctgctgg tattttaagt
     3361 ttgaaaactt tcaaaatgac ttgtggcatg aaaatcaatg caatccctaa taaagattcc
     3421 acgataaatg tgattccttg agaagtcgaa tatttttgtg tgataaattc ataaaaatta
     3481 tagcagacaa cgaatacacc aaaagcaagt aaagctattt gcaccttttt gatttcttta
     3541 atcactgaaa gtccctccat ctaaaaatct ttttacttct gaaagcaact caatttctga
     3601 agaaaccact tttccgttca atttgatcaa acctattgta tataagttca ggtagtgaaa
     3661 ttgattttca gctgtttctt gtagtgcagc gatttttttc gggtgggttc cgccttgctg
     3721 tcgagtatca gtatataagc cgatcactgg gatgcctttc gcataagcca caccgatttc
     3781 cgatgctacc ccggcatcga tcgtcagccc atctagtaaa gcaatcatta aatcactagc
     3841 caacactttt tctgtgtcag caagagcaat cattcgactg tctgcataag cagttttgtc
     3901 attgattgct tcattttctt gaggaagata gatcgaaagc tcagaagaga gctctctgat
     3961 ttctttaact aactgttggt tatataacaa atcactttta gcaaacattg gtgcggcaaa
     4021 ataaattttc atctaaacat catccttttt atcaaaaata atcgctaaag taagcttcca
     4081 tgtttggaat gatacgctct agtgacttca gtgtttcttc atctgtttct aaccgttcga
     4141 ttcttgccaa ataagaagca cggcgataat tcatcatcaa gcaagtcaat gtttggatat
     4201 ccagtcgaac ggattttcct ataggttcat ttaagacacg gacttgtccc tgttcatccc
     4261 atgttaaagc gaagacacca ttattccaag gagcgacagg atcttcaatg atgaaatgaa
     4321 aagcatcagc tgttccaaca aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc
     4381 gagccataaa atatggttcg atctgctctt tgatctgact atcttcaatc aaaaatgcta
     4441 aaggctcatt tttataaata tctccatgta cccagtagat catggaaaaa tgtgcggaaa
     4501 taaagttcca taagccattt cgagcttctt gatcaagata gaacatttct tttacgtgaa
     4561 atatttcttc agctacccag tagaacagaa cacctcttgg ttcatggttg ctatcgtagt
     4621 agacagcggc tgtgcgctca tcttcatttt cgaaacgcca atactcttcc caatgaaaat
     4681 cactgcggaa taaagcacca tggttttgtt gggcaaattt gtcataaacg gaaaatacgt
     4741 ctggatgatc aactggtaat cgctcaacca taccacttac tgggatttgt ttaggtagct
     4801 gtgtatctct gattttaaag gaaagtttat cagacatgat ttcccatcct ttacgccgat
     4861 agtaggggat actgtaagga tacaaataag agatccattg cttattcttg cgcatattat
     4921 ccaatgcaac gataattaga tcttgcatca atccatggtt agcatattct ggataagttc
     4981 caactccagt cacaccaccc attttataac gtgcaccgtg aatattgact tcacaaggat
     5041 agatagcgat ttgagagatc agtttatttt cgtggaacca tccgaacact ttcgatactt
     5101 ccaaaatcgg ttttttggaa cggataaaag ctcgtttatt ctcaaacccg ctttcctcga
     5161 tgtctgcttc agtcacttgg aacacatagg aaagcaattc attgaattga tcgatatgtt
     5221 cttgagtgac cggttttaat tcaagatttt ctctaaaatc tttttgatcc attttttcat
     5281 ctccatttat tagcttgata acagtatagc aaaattctac aaaaaaaact tctatagctg
     5341 actaattaaa aactctttaa attactgctt ttcgttggta taatggaaag tgctgattat
     5401 atgaaaagag gagacgctat ggatataaag aaaatgaaag aacgccaaga gcagattcgt
     5461 aatttttcga tcatcgctca tatcgaccat gggaaatcta cattggccga tcgtatttta
     5521 gaaatgacgc acactgtgac gtctcgcgaa atgcaagatc agctgttaga ctcgatggat
     5581 ttggaacgtg aacgtggaat cacgattaaa ttaaatgctg tagaactcaa ttacacagcg
     5641 aaaaatggag aaacatatat ctttcacttg attgacaccc cagggcacgt cgacttcacc
     5701 tatgaagtct ctcgaagcct tgctgcctgt gagggtgccg ttttagtggt cgatgcagca
     5761 caaggaatcg aagcacaaac attggccaat gtttatttag cgttagacaa tgatttggag
     5821 atcctgccag tcatcaataa aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa
     5881 gaaatcgaag acgtaatcgg aatcgatgct agtgaagctg tattagccag tgccaaatca
     5941 gggattggga ttgaagatat tctcgagcag atcgtggaat atgttcctgc accaagtgga
     6001 gacttagatg caccgctaaa agctttgatc tttgactcta tttatgatag ttaccgcggt
     6061 gttgttctaa atgtccggat cacggacggt atggtcaaac ccggagacaa aatcaaactg
     6121 atgagtaatg gcaaaacatt tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt
     6181 gctagagact ttttgatggt cggtgatgtc ggttatatca cagcaagtat caaaactgtc
     6241 caagatacac gagtagggga tacagtaacg ttagcagaca atcctgccgc tgaagcttta
     6301 ccaggttacc gaaaaatgaa tccgatggtt tattgtggtc tttatccaat tgatacttct
     6361 cgttacaacg atttgcggga agctttagaa aaactgcaat tgaatgatgc tgctttacaa
     6421 ttcgaaccag aaacctctca agcattaggt tttgggttcc gctgtggatt cctcggactt
     6481 cttcatatgg atgtcgtcca agaacgcttg gaacgggaat tcaatctaga attgatcacg
     6541 actgcaccat ccgttattta ccatgttaat aaaacggatg gtacgactgc tacagtagat
     6601 aacccggcag attttcctga acctgtaacg attcaggatg tagaagaacc attcgtcaaa
     6661 gcacagatca tggtcccaaa tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa
     6721 cggggagaat tcattacgat ggattattta gatgattacc gagtaaatgt cgtctataat
     6781 attccgcttt ctgaaattgt ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc
     6841 tatgcctcat tggattatga aatgtctggt tatcaaaaaa gcaagttagt gaagatggac
     6901 atcttgctga atggagaaaa agtcgatgca ttaagcttta tcgttcaccg agactttgcc
     6961 tatgaacgtg gaaaagcaat cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt
     7021 gaggtgccaa tccaagcagc tattggacaa aaaatcgttg cccgttcaga catcaaagcc
     7081 ttgcgtaaaa acgtcttggc taaatgttat ggtggagatg tttctcgtaa acgtaaactc
     7141 ttagagaaac aaaaagaagg gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg
     7201 caagaagcct tcatggccgt tctgaaaatg gatgagcaag atagtgtgaa ataacttata
     7261 atcaatcgaa aaacctatta aataaacctt tttgagtaga tgcaacactg aggattatct
     7321 gtataaaaga ttttaaactg tcatttggtt atcacggcga tactaaaatt aacaaaattg
     7381 gaatattttt cttgaagatt tttagctgtc agtatatgat gaaagcatct cataggaggt
     7441 gcttttttca tggttgataa ttcaagtgga agggttctta aatctctaag aaaagagaaa
     7501 aaactatctc aaaaaaagtt agctgattta gcaggtattt cacaaagtac tttagttaaa
     7561 tatgaaaaag ggtcaagaaa aatccctaaa gacgtagata atactctaag caagattctg
     7621 aatatagaaa ctctaataaa agatgaggaa gataagattg agattctcat cggtaaacta
     7681 attgcataca gagatatgaa taagttactt aacaaagagt tagctgataa tataggaatt
     7741 agtgaagtat tactcagtta tgtgttaaat agaaagagaa atccgtcaaa agaaatgcaa
     7801 aaaaaaatcg atatttttct attaagtaat gaaaaagaaa ttttgaagga gattaatcgt
     7861 gatagtgaga tatttagttt atcaaaagac gataaaattg ttatgggaaa aagaatacga
     7921 gaagtccgaa agaaccggga agagacgctt gaaaaatttg gtaaaaactt taccatctat
     7981 acaggcaaaa atgttataag tagatgggaa aaaggtatta atattccaga tattgaaaaa
     8041 ctaatgaata ttgcctatct tggtaaggtc actgttcctt accttatgta cggagaagat
     8101 tataagaata tacttccaaa agatgaaaga gttagcgatt ttaaaaagat aaattctttt
     8161 agtatgggat taaggatgag gaaaattagg aaagattatt atctagaaag ggaggagttt
     8221 ggaaagcttt tttctccttc aatttcaaaa tggtccatag ataggtatga gaatggtaga
     8281 gatataccca atactaatcg aattatccaa tatgcttata taggaaatct ttctttagaa
     8341 tttttgattt acgggatata aaaatagttt acgtcgatac ttattaagtt atcgacgttt
     8401 tttattatag aaaaaattta taaaaggagg tttcttgata tgataagaaa caaaaagggt
     8461 ttgttgaaag ttgctttact ggcagctttt ttctaagtaa ctttttgaca agctcaatgg
     8521 caacacaagc ggaagaagta atacaagaac taactgttat gaataacagc tagataaaat
     8581 gggctatgta tgttatccaa caatttagta atcactagaa gcctagcgaa agttcgcttg
     8641 ataagttata gatgagtatt gatttgtagt gtttccaaat caaaagggta cagagccaca
     8701 tgaaatatgt tgtcctctgc agcgtcagga tgggaatcca taccatggag attcggagat
     8761 ctgtgaggga atacttacgt ggttaatatg tcagttgtgt tggacgtagt aatggtcata
     8821 gtgggtagat tgactgaatt cttatatgag aaaatgaaac caacaagaag atagggttat
     8881 ttatacagaa ttggggatct tttctggaaa agtaatcgga taatgcgaga ttcgtacggt
     8941 cttttttaaa tttctctaaa aaaaacggca tcgaatgggt tgggtgattg ataagcaatg
     9001 aatcatagac tgatatagaa taaaaaatga ttctatttat ctttttacat ctatcgtttt
     9061 ttcaagtagc cgcaaattat atcagcattc aacaattttt gtatacaagt tctagtaaaa
     9121 ggatctgaag aaaaagttgg ttgttgtgaa gataaaaaaa gaatcaggtt ttactgagtt
     9181 tttcttcaaa atttgtgtta ttaggttagg acaaagataa gaagaagtat gaagatttaa
     9241 aaaaggaatg aggaaaagat agtaatttcc gattattgga gtttttttat ttataaagaa
     9301 gaagcgaata agttgtgatt cctagatttc gcgcgtgatt aattaatgag caaaaaatgc
     9361 ggaaagtttt agtgtttaat acacgaggac aaagtttctt aggtattgaa ggcacacgtg
     9421 taacggttga tgggtggtgt gattgtttcg aggttggaaa acatgttgaa cttatggcat
     9481 ttactgagct aaaagataag aacaacgtgg aatttttcga gagtgatatt ggttgggacg
     9541 atcatgaaga agtttatgga aaatttattt tgcggaaggg aaattcttat acgaatgggg
     9601 aagcataagt agcgatttgt tagagatcat tgataggatt gaaattgtcg ggaatattta
     9661 tgagaatcta gagttatttg aggtgataga atgagtgatg gttttgaaca agtatcaata
     9721 attaaaaatc aagttcggga gattcttaga aaaaaaggct atttggtaga tagttatttt
     9781 gaaggagatt acgaaacatg ggtaggtgtc tatgctcgtc cagagaataa acctacctat
     9841 ttagatccta cgacgtcaga agatgcctat ttacaaaata ggtatcgagt agatggtttt
     9901 aagcaagact ttgcggagtg gtttgaatgg gaaattgaaa acggagaagt aaaagaagaa
     9961 taagagtact tgttgtatca aaataagcca gtagaacaac caaaccagta gctagtaatg
    10021 acaataatac tgaggaatcg gatcattatg gtatgattga tatttctctt ccagaagatc
    10081 ttaagccaga tcctgtaaca attggcaata gtgtcatgat ttttgattca ttagatgaag
    10141 ctatgtcata tggttggaaa gaaattaagg atgaaggtag taattggtat ggcatgagat
    10201 ttggtgatgg tgaagttgcc aaaaaagctg gtattccaat tgggaaatgg acagttgatt
    10261 tccacgaagc aggttctttt tttgttctgt aattatagtg ggagggtaaa atcattgatg
    10321 aaatgtgttc agataagagc gccagataaa aatagaagag acagttcacg tcgttatcac
    10381 ttttggtaac ggcgttttgt tttattatat aaaattctca ggaaaaggag gaattcttgt
    10441 gtatgcaaaa acgaaaatga attggttgaa agcactcgca ttggtggctt tttctcaatt
    10501 ctgtttcttg gtgggaactg ctcaagcaca tgcagaagaa acgacggcta tacaacctac
    10561 gatggaagcc attgcccaag tcgcttctga agtgacagaa gtccaaccag cagaagccgg
    10621 ggtcccagct ggtgaagcca ctgttgaaca ggcccctgtg acagaagcgc caacggtcaa
    10681 ttccaaaacg actctcgatc agacgattgg ggaaacacaa cacccaggga atagtcctgg
    10741 tacttcacaa ccagttgaag aaactgcctc aattacaacc cctgcttcag aaacgtccac
    10801 tctagaagga gaaaaagctg ccgaagcaac gccttctgtg cagccagagt cttctgagaa
    10861 acaagcggaa gaaaagaaac gaatagaaga aacagtggaa caaccagcgg ctactgctga
    10921 caaagaagga aaaaatgagg aaaacgttga aaaaacaatc tcttcaaccg cagctagtca
    10981 aaatcaacca acgaaaccaa acaaagaatc tacggctagt attgaaaatc aaaagaaaaa
    11041 agagcagaac attccaaaaa caggtacacc tacactgtta gtaacagtac caaccaatcg
    11101 ttcggttcgt gttcgccgtg caaccaatgc agctgtttta ggtggtcgga aaattgattg
    11161 gggcgaaaat attggtgtta cttctagtcc gaacaatcta aacttcactt taaattatat
    11221 ggttcgtttt gagattaatg gacaagtggc gttttgtatt gagcctggtg tctcagccgc
    11281 agaaggtcaa gtttatacag ctactgcttt gaaaaactat ttgaaaaatt caaatgtacg
    11341 gaaaaaaaca agcttgatct cttatttagg atatgtcaca aataaagaca aatccgatga
    11401 acaatacatt gcggcacaat ttatgatttg ggaagctttg ggcacaaaaa tcccaatcaa
    11461 aaatatacgt gttgattatg atcgtcgaaa agcagaaatt caacgtttga ttaataactt
    11521 tggtgaaaaa gcaactttta ataatcaaac acatacggta aaaattggcg aaacgttgaa
    11581 agtaaagaat aaaaatgatt ttttgaaata tgtgaaagac attcaaaaac caaaaggtgt
    11641 caatgtatct attcacggca atgaagtatg gattacagtc acaaaagagg caccggataa
    11701 agcaaaaatt caattgaata aaattaaagt tcaaggtgcg ccattagcct atcgtcgtcc
    11761 aggagctcaa acaattggtg tgttacatcc aacagaacct aatgcgtctg ttattaattt
    11821 acaaattcta aaacaaggaa acattcgtgt gtttaaagaa gatgcggaaa ctggtgtgaa
    11881 agcacaaggt gcagcttctt tagaaaatgc ggtctatggt ttgtatcaag cagatgggaa
    11941 aaagattaaa gaaattacct tgaaaaaaat taacggcaaa gtacaggcag aaattaaaga
    12001 tcttgatcct ggcaaatatg ttttaaaaga aatcaaagcg ccaaaaggtt atgtgctttc
    12061 tgatgaagca attgaaattg ttgttgaacc tggaaaaact gtcaatgcga ccgcaaaaga
    12121 tcaagtgatc aaaggaacca ttgatttagt gaaagttgct aataaagatt tagtcgattc
    12181 aacgaatcca gataacaaac caaaattagc tggcattgaa atcactttga cgtcaaaaac
    12241 aactggcaaa gtggtgaaaa ccgttgtgac agacaatgat ggttatgcta cttttggtaa
    12301 aaataccgtt gtctttgata cgtacattct ttctgaaacc aaaggaaaag aagggtataa
    12361 actatttgaa ccacttgaag tcacaatttc tgaaaatggg caaactttcc attatgtttt
    12421 agaagataaa gtaattgaac aacgtcttaa agtggtgaaa gtggatcaag aaacaggaaa
    12481 agtgattcca cttgctaata cagaattcaa gatttttgat actttagcca atcgttatgt
    12541 cacaatggag attccaaatg atacagaaac aacggatatc tttaaaacca atgaaaaagg
    12601 ctttttccta actaacggta cacttactta tggaaaagat cgctatcgtt tggaagaaat
    12661 caaagccccc gtaaattacg ttttgaatca aaacccatta gtgttctcga ttaccaatga
    12721 tacagaaccg atcaaagtga tccattttgc gaaccgtcaa gcacggaaaa gtgtaaaatt
    12781 attcaagtat gaagaatgga acaagaagaa aacgccgctc gcaggtgtga actttacctt
    12841 gtataacggt aaaaatcaag caattggtga atataccact gatcaaaacg gggaaatttt
    12901 agtgaaaggt ctagttgccg atgactatta cttcttggaa aatgccccat tagaaagttt
    12961 ccaaccaaat acagataaac aagcattcac ggttgcattc aaacaaaatg cgccagatga
    13021 tggggaaacg ttagtgattt ctgtacgtaa ttacttgatt ccaccaaaac tagaaacaca
    13081 agcgtcaaat aaagccgatg gtgaaaaagt ggttgatcca ttagaaacga tcgtcattaa
    13141 agacagtgtt tcttatacca acttgtttgt cggaaaagaa tacaccattt caggagtatt
    13201 gatgaacaag gcaacgaatg cgccaatttt acaagatggc aaagaaatca caagtacctt
    13261 ggcctttatt gcagacaaag tcaatggcac aaaagacctt gaatttgtgg ttaatgctag
    13321 tgttctgaaa ggtcaaacga tcgttgtttt tgaaaaactt ttccgtgatg gcattgaagt
    13381 tgcagcacat gcggatatca acgatgaagc ccaaagtgtg aaagtgaata atccaaccgt
    13441 tcataccacg gctgtctttg aagacggatt gacagtcgca gacccaacag gattgtttac
    13501 cttaaacgat accgttgaaa ttaccgagat tcttccagga aaagcatact tggtcaaagg
    13561 gatcttaatg gataaagcaa ccggtgaacc cttgcttgtg catggaaata cggtagaaac
    13621 gagtgttggc tttattgcca aagcagaaaa tgaaacggtg atcgtaccat ttacctttga
    13681 tttgacaggt ataaatggtc gtgaaatcgt cgtgtttgaa agtcttttta gaaatgatga
    13741 agaactagca aaccataaag acatcaacga caaagggcaa acggtacgag taactaaccc
    13801 aagtgtacgt acaacggcta cacataaacc cactggcttg aaagaggtta atccattaac
    13861 gaaagtcacc attacagacg ctgtgaaata ccacgatttg gtcgttggaa aaacatacac
    13921 agtcaacggt gttctaatgg acaaagtaac ggaaaaacca gtgctagtag atggaaaaga
    13981 agtcacaagt accttaacgt ttgtggcaga aacaacggat ggtacagtca atctagactt
    14041 tattctagac gcacgtgcgt tgcgaggcaa agaagtcgtg gtctttgaag atctgtttag
    14101 agaaggcgta ttattggctt ctcacgcaga tattaccgac aatgaccaaa ccatcaaaat
    14161 cagtaatcca accattacga cgaaagcgac gaataaaaat ggcggaaaag agatcttggc
    14221 cttgccaaat caaactgtgc ttgaatgggt caaagtagac ggtcttgcga ttggtcaaat
    14281 ctatcgctta attgttcaag gctacttaac accagacggc acaccatttg aaggcactca
    14341 tgcggaaaaa gtctttgcgg ccgataaaga aatgatggaa ttcttatttg aattcttagt
    14401 ggacggtcgc aatcttgcag gaaaaggctt gagttttgcg gaatggctcc aagcgaaaac
    14461 ggaagatgag aaagaaacgt ttgaagaagt ggcgaaacac aatgtagatc taacaaacaa
    14521 agatcaaacg gttcaaattg tggaagcgcc aaaaccaccg caaccagtga aaccagaaca
    14581 acgggttaaa ccagctactg ttgtaccaat gttgccaaaa acaggtgaac aaacaagtag
    14641 tatacttatg gttcttggat tagtagtatt agtcattgct ttaggtataa cactttataa
    14701 agtacgtaaa gccaaagatt agtcatgagg aacaagcggt taggattttt aaccgcttgt
    14761 ttgattgtaa tgtagaaata tttagaattt attttggttt ttaagaggag gaaaaacttt
    14821 atggcagaaa aagcattgcg tatgaagaaa gagtttttag gtcaagtatt tttattgggt
    14881 tcacgggaac ctaatccacc aaaggaaatt aatggtgtgt ctgggcgata tgttcaatta
    14941 caagcagagt gttcgcgaga agaacagcct gctttttttg tttatctcac agctgaagag
    15001 gataaaagat tacgattacc agaaaaagag gtgcgtattt catttgaaga agcttatttt
    15061 gtttttcgta aagaacgagt gaatagtcgt gaaacaaatg atatgggaca aacagagcgt
    15121 cgaacaactg gctatcaaga tgttggtgtc tttttaactg gtgtaaaaat tggggaggtt
    15181 ttgtaagatg ggattaaaat ttccaaataa ttatgacatt acagaagcat tagatgaaac
    15241 ttgtttagga gccgtacgct ttggcgcatt agaagagtct gatcgtgtat ttgataatga
    15301 aacaggcgat tatacgggag aaattcgatc acagcgagca caattagttt ttggaaatcc
    15361 agatgaacca aagattgcac gacgagttaa atttccagca gatgtagatt tgactggttt
    15421 taaacgacgt gatcctgtga aattaattga tccagaatat catgtggtat atattccagc
    15481 acaagatggc aatcgtgaat attcagattt agtgatttat gcgaaaggat tagagaaagt
    15541 agaagctcca acaaataatc ctaatatgaa acaatcacaa gaaaaaggtt tagatcaaaa
    15601 acaaccaagt gttgattcta aggaaaaaga gaaaaaataa ggtttttttg aaagaatagg
    15661 aaggagtctt cctattcttt ttatctaaga tagaaggaag gggagatttt gaattcagat
    15721 aaaaccaatc aaatactttg agagattgtg cttgttatca aaatatccaa caattgattg
    15781 agtattttgg agacaatcca acgatttttg tacaaacaaa tgcatatatt tgtgcggtta
    15841 ttgggcagtg tatgttttta cgtgatcttg ttcgtttttc attaccgtac cagtggaaac
    15901 aagatcatca ggaagtgatt tggattcgta tagtacgaat atgtaatatg tgtgcacacc
    15961 cttataaaaa tcgattaaat ctttttcgat tatgtttttt tactaaatgt gatattcctg
    16021 tattagttgc tttttgtcaa aagtttttag cacaggaaaa gtaaaaacag aaaggaggaa
    16081 tgtgacaatt gaaagctcat tttttcaagt atcgtggtcg gcgaattcgc tatttcaatc
    16141 gttatttatg gttttgctat tatggtttct ttttgtttcc atttttgatt ctgttaggtt
    16201 ggtttagttg gaaagtattt tatccacgtt taagtacgtt ttcgaattgg caaatgtatc
    16261 ttattcctgg aataagtatt gtgttgtatt tgttgctggt tagtgggtta attttagggt
    16321 tgatgcattg gtcgcgacta aaagaaggat attttgcctc tgtttattgg cgtcagttac
    16381 ttgttcgaat gttaattgac aatcggtttg tttatacaaa gaaacaagtt agtgagaaac
    16441 aaacaaggga aaaaatgaga ttaccaaaag tttattttta tcaaaagaaa caagaattag
    16501 tggtttcttt tcctttagat ggtgggcgtt ttcacgaacg atttttaaaa atgggtcatt
    16561 tgttatctga agtatttttg cgtgatttaa ttcgtgaaga acgagaaaaa gcccgaatca
    16621 attatgtatt cctttcagat tctggtcgga ttcctattga tcaatgtata gcagaaaatg
    16681 gacgtattca tttaatgaaa actttggatt gggtatatga tcaatcacca aatatgttga
    16741 ttgctggtgc aatcggtggt gggaaaacct atttattgta ttctttaatt caagcttgtt
    16801 taagtgttgg gacagtagat atttgcgatg gaaaagcggc agatttagca gcattaggtg
    16861 acgtcgatgt attcaaagga catgtgtttt acaaaataaa tgaagaaatg attatttgtt
    16921 tacgaaatgc attaaaagaa atgaatcaac ggtatgccta tatgaaaacc atgaaagatc
    16981 ctaaatatga accagggaaa aattatgctt attatgggtt accaccgcat tttatctttt
    17041 ttgatgaatg ggcagctttt tatggttcgt tggatatgga agcgagaaat agagtcgata
    17101 aattgattca gccattagta ttaaagggtc gtcaagcagg gatgtacatt attttagcaa
    17161 tgcaacgacc agatgcagaa tattttccaa gtggtgttcg ggataattta acattccgta
    17221 tttctgttgg tcgattgtct cccattggtt atttaatgac ttttggagaa gattttaaaa
    17281 ataagccatt ttacaacaaa gatcaacctg gtcgtggata tgcttttcca ggatttgatg
    17341 tacctgtaga gttttttgcg ccatttgttt acaaagatta tcactttatc aaagaatttg
    17401 agaaatttac tgaaatgatt gtctatgatt ttacagtaaa agaggatagt caagatcaaa
    17461 cgatgggaga tgataatcag ttgttacaag aacaactaga gcgccaaatg tatttagcaa
    17521 aagatccaaa aggaacaagt ttttctgatg cgcatatttt gtaatcaact aaggaggtga
    17581 agttaaatgg cagagagttg ttattataga gtagctacag caatacgaat gattaatcct
    17641 tctctttctt ccagaacgtt ttatgattgg ttgaatcgta ttgaacaagt aactgattac
    17701 cgctttttgc gcaaagaacg tgtttttaca ggaaaagtta ttaatcaagt attgctaacg
    17761 aaaaaagata tagaacgatt gactcgattg tatcattatc gagtagattt agaagaggat
    17821 ttaactttgt caatttatcg cgttttttct cctgaaaaat atagtgaaat aacaaaatta
    17881 gatcatttaa ttttgtagtt attatctatc gggagggctc tagacgtagt ctggtagccg
    17941 cactctgcct ggcggtgcgc attaaggctg cgaagcatgg gcctataatg cgcactcgct
    18001 agcagcgtgt gcagctcaca aagaggtcgt gcgcttgcgc acggcctctt tgtgtaaagc
    18061 cccccgatat ataatatggg gggcagaaat aacataacac atagaaaaaa gttttataaa
    18121 cccttttgta tcaagtgatt atctaaggtc agcagttgta ctacttttcc caaaaaacga
    18181 gggggactag ttgatatatc ggtgtttcta aagggttaaa aaaaatcgat tttgttattt
    18241 tataaaagga gcgagaaaat gacaccgatt gagctaaaaa gctacagaaa gaagttaagt
    18301 gcaattcata atataagtgg agataaaaag ccgctatcac aagcaagatt tgcgcaattg
    18361 atgggctgct caaaagatta cattagaaag atggaacaag ggaaaagaaa aatcccagag
    18421 gattttgaac aacgaatcaa ggaacgcttt cgattgtata tgaaaagcgg aatagatctt
    18481 cgagcaatga ttgattattt gcgtctttcc ttttttggga cagactacaa aacagtgatt
    18541 caacgtgtat tacgaatgga tctgacagaa tttgaaacac gtccaacggg cttgtatctt
    18601 tttgactgtc tttctgttcg tgggaatatc tgggtgttct ggcattcaaa acgaactgta
    18661 acagaaaatg tacttgtgca attttcagga caaggttgcc gggaatatga ggaagtaatg
    18721 cgtgaacaac aaacagattg gcgaacatgg ctatattcca tttggaaaaa tcaaggaaag
    18781 ttggaagctg gttatacacg tgtgcaatgt agccgattcg atattgcgtt agatgaactt
    18841 tggggattgg aaacggaaga acatttcgat ctctttgaat tactagaaaa acaaaaagca
    18901 ggtttattag aaatggactt taagagattc cagaatattg gcggttgttt tttagatgat
    18961 ggctattttc gaagtgaagg attatctttg tattttggat cgcgaaagag tcctctcttt
    19021 tttaattttt atgagaaacg ttttgaaatt gcgaaccgag aaaaaatttc agaattagaa
    19081 gcattaacga aatatggtat ttataatcga tatgagttac ggttagccaa tgagaaagca
    19141 acacaagcag tagaagcatt tattttaggt gattcaccga aacgtttagg agagattggc
    19201 gttggtttaa ttaatgcaaa acttattgtt tgggctagac tggaaagtga tgcacggatt
    19261 gttgataaac gatgggcaaa attatttcgt tcgatggaag aattggattt tacagtaaag
    19321 ccagaaaaat tctcattaga tcgttcagaa cgctggtttt tgaaacaagt agctccgaca
    19381 ttaaagttgt tacaagaagc agatcgtatt ttaggaagag actttgtaca agaagggatt
    19441 gataatgccg aactaaatga cgagcaacaa aactatctag aatggatggg aacgtcagga
    19501 gggaaaattg catgaatgca tatacgacaa cagaatggct gcgcctgttg gatttaaagg
    19561 ctgcaatcga agcattaaat gaaaagatgg ttgatttgtc ttattttcgt tttcgagttc
    19621 cttatattga acaagcggta aaagctggac gttatcagga aaaagagaac tggcaagaaa
    19681 ttgcacgctt attagaagtt cgtaagggat acgaacaaga actcgaagag ttggaattta
    19741 gccgaaggaa aggaaggcta gaattcattc gcttttatcg attttcattg ccaatacctg
    19801 cgattttagc ggttaaaaaa ggctgcgata aaatgaagat atatgaaaac tgtgtggcag
    19861 cacttagcag cgaaaaacct cttatggaag aaatttcatt ggtgactgtc acttgggaaa
    19921 tgagtcgcca gccaacagaa gaacagacat atctttcttt agaagagatt gagatagaat
    19981 tagaggaaat tggtcgctat gccacttgtt ctacctattg tggatcggtc attagtattg
    20041 ctggtgtgat tgtctaaatg aaaaaaatta tgatttatgt tggtgcgtat tggtcacgtg
    20101 atccaacagt attagaaaat ccagaggcgg tacactattg cttgcgccaa ttattctatc
    20161 tgtataaaga acgattagaa tcgttgattc gtcagttacc atatactgac agacgtttag
    20221 atgaacttct tctaaggtat ccagcaatgt ataaacgacg aaaaaataga ttgttaccag
    20281 aagagtatcc cattgaaaaa catgaacttg aagggcgatt tgtggcttat ttctatgatg
    20341 atgttcgtat gcgcttagta gaacaacgaa tggaaataga aaatgatcgc tactatttta
    20401 tcaattattg taagaagcgt aagtatcaag taactgatga tttttatgat tgtattttac
    20461 aagatgggga agtgattcta tccaaaattg ctccaagttt tcgtgaactt gtgccgattg
    20521 attttttgaa aaaatgtcat ttgcggattc ttccgtaaaa aagagctata aaaggaaaga
    20581 aaagtagaag ccaaatcggt ttctgctttt ttattttttt taggaaggag aagatggtat
    20641 gtcactggaa tttttaggag agccaattga aaaggaagag ttcgtttcgc attatatgga
    20701 tttgtttatt caggtgataa aaaactttgt gcgattgatg aattttatcc acgcgaacaa
    20761 gactatctag ccgctgaacg aataaaaatc agtttattat gggaaaaatt cacagatgaa
    20821 aataaccgac cgccagatta tcgctattta tcagattgtt taacgaataa tgtgctttct
    20881 aaagaatacc tatttcacaa acgtgagaaa aatcaaatgt cggttgaaca tttttgtgat
    20941 tgttatattg cattattgcg tagtcaacag ttattaacag tagaacgata tggtgcagag
    21001 tttggttatg tcttgcaagc tgagcgtcat agagctacaa gatgctatga aaaagaagat
    21061 caagtgattg atggctatga agaattatgt attcgaaaca atcgctattt gcaacaacgt
    21121 ttatatgaac aattgatgat ggatttttca aatctctata gagggtatga atgttcgatg
    21181 attcaagaaa ggtagaagaa aagggggaat ttttatgtgg cgattattat ttttggtggt
    21241 atgtggtatg gcattgttct atgtgtatcg tgtctatctc tatccacgaa tggaacgatg
    21301 gacaagttgg tatagagatc ctttaaaaca acgaaaagga ttgaagcggt atcgttatga
    21361 attacttgtt gaattttcgg atgggcgaca agcacagtat tatttttttg caaactatcc
    21421 aaaatacaac accagtgaac tattgattca attattcaaa aataatccat atgttgggtt
    21481 agaagaaaac ggacgggagt tcttttatcg ttcagataat attagtgcgg cgtctatgaa
    21541 caaaacacga attgcataaa aaattggagg aataatcatg aaaaaaacaa cattagcagt
    21601 cgtaacaaca ctaggaattt cattattttc gtttcaatta gggagttcat tggtttatgc
    21661 agatgaaact gtagaaacac ctaagtcaga aattgtagaa acattgccag gggaaggatt
    21721 accagatgtt gagacagagc taaatggagg aacaacggaa aaaccagcag aacctgaaca
    21781 attgcagtca gagaaacctg aaaaagagcc agtgacacca gaaccagagt ttcctgaatc
    21841 aactttaccg actgattcga tcgaagaaga aggtgtgcat agttcaacaa caacggattc
    21901 aagtatagtc acaactccgg aacaatcagg aacaaccagt tcttctgaaa agaatactga
    21961 ttcgtcaagt acggaacagc caacgatgtc gtctaccagt gaagaacaaa aagaagaacc
    22021 gaaacaacct gttgaaaaat cagaagcaat tccaaaagaa aataaaccta ttgttccagc
    22081 aaaagaagtc acagtatcgg taaccccaag cggtgaaatt acgacgaata cttctcaagg
    22141 aatgagtgta ccgattgtca ctagtaatgt agaggaattg acccatattc caacaccaac
    22201 caccccatta aaagtagcag gtgatcaaac aattgttggt gtaaaagatg gtattccatt
    22261 aatccaagat agtgaaggga atttggtaaa ggatttgtcg attcctgtaa agaaacttcc
    22321 aagtggcaat attgaaatca aaacagcaga tggaaaaaca aaagtcttac caaagactgg
    22381 tgaagaaata catgttgcac tttctgttct aggaggagtg ttgacaagta ttgctggatt
    22441 tgttggatat aaaagaaaga gaaaaggaat agaaaatgag ggtaattgaa tggactattt
    22501 ttcagaaaaa caagcttatc gttgtcaatc gtacgtacta gtaaaaacat actagaagga
    22561 gtagcttatg aaaaaacgac aactacggcg gaaagaaaaa gtttatacag agaaagtacc
    22621 gaagcaaaag aaaatccatt caaaaaaagc agtgaaatgg atttggtgtt tggttttatt
    22681 tctttgtttt tcagggggct tggcattttt tcaatccatg aaaacgaagg ttcaagtgtc
    22741 gtttttgaaa gatgaaatcc aacaattaca aaagaaagaa gtacaaagtc ggtcagaatt
    22801 aacgtacaca cctgaaattg aatcctttat gaatcgtttt gttgctttgt atatgaatac
    22861 attcgataat catgaacaag aacaacaacg tcaagaaaca ttaaaaaaag agttttatgc
    22921 acaaaacttt attcaagaac caggaagtgt ttcaggtgtt cgaaaattaa ctgctgcttc
    22981 gtttattgga ttaaaagaaa ttgaaaccat tccaactgct agttataaag taacttatga
    23041 tattcgctcg atgaatccag agagtaagaa agaaacaagt gtgacaatca cacaagtact
    23101 caatcttccc ttttctattt caaacaaggg ctgtcgtatt attgcttatc cttattttac
    23161 ggcactacca aagaatacag aaaaacaacg aatgattact tatgatgaca cattatacga
    23221 aacggttgat gcgtcggaag cgaaagatgt tgaaggattt gtggaagatt tcttagataa
    23281 gtatgcgaaa aacaatgcaa aagatatgtc atacatgatg aaagaacctg aaggactcaa
    23341 tcaaacagct aaagttgatc aagtagagat gaaagtattg cgttataaag aaacgaaaaa
    23401 accttttatt gtaaaagcaa cggtcgtctt tcgagatggt gatacgtcac ttttacaaaa
    23461 agaacaaatg acgattgtca ttacgaaaaa aacaaatcag tattatgttg aaaaattaac
    23521 acataattgg ttggatagga aaggagaaga acggaaatga cactgaaggg aattgtagat
    23581 tttatattaa cgcaaggtgg ctatttttta ataatggtgg ttgtttatct gggattgcga
    23641 aattttatga aatcaaaagt aggaaatgca ttaatatcta tcgcagcagg tgccgcagtt
    23701 tatttcttct taaatgatcc aacacgtgta tttgatgcgt ttggtaaaat cattgaaaaa
    23761 ttatttatgg attaggagga tagtgaagtg gattgggaac cttatgatta ttcgattgtt
    23821 tatgcggaac cttatcgcgt acaacagatt acaagcaatt ggcgtcttcc atttccagtt
    23881 gcattagtca atgcagcaac ttttttgttg gtcgtaatct ttgaatattt atttttacgt
    23941 tcatttattg gttgggtcat ttcttttttt ccaccagcag gagttgtttt ttatatagga
    24001 ctcccctatt tatgtgtgtt agcactggca aaactaaaac ctgatggaaa aaaagtgcat
    24061 ttttatttat gggattggtt ttgttatatg attcgtatga tgaatggtga ttgtgtgtat
    24121 tataactggg acaaggtatc aaacaatgca ttggaagaat tactttttgt gaataagtat
    24181 gtttatttag aggattagaa aaagggacaa tcaactggtt gttccttttt tattgggagg
    24241 tgaaattcat tggtgcttgt aaaagaacca gtaaaagcat tcaagaaaaa ttgtttgtta
    24301 aatggagagg attgtatctg ggcctactat catgtttttc cacgaacaat ttcaaaaaac
    24361 acaccaaaaa agacacagga aataaaagaa atgatggctc gtttactgtt gcttttagca
    24421 gattatgaag atgtggagtt ggtgatgtat ccacgaacaa tgaatttacg gacgaagttt
    24481 gacacattag gaaagggatt ttctcccgat agtcaagcaa ttggtgcgta ttatgcaaaa
    24541 gaaacagtga cattgttgga acgagaaggt caagtctatc gtccttcatt tattttagga
    24601 atcaaagtca aagaaaaagc aacggtggag agttttcatg atgtaataaa tctaatctgg
    24661 ttagatttag caggaagttt agcacgctta tttggattag aagtagatga tttggaaact
    24721 ttgttttctg aaagtgatcc ttatgtaaaa gaagtgacac aattacttta tagcttttct
    24781 attcgtccct tagtaaaaaa agagctgttg tatctcaatc gtctgcaata tattcgagga
    24841 atggctcaca aagtagaaga acaaaataat tgggaagtac tgaaagacaa tcaaggaacg
    24901 tatgtacgta taccgaagga acataaaggc cttcttcaat tagaagatga gttagggagt
    24961 agttatttag cattgttgcc tattggggaa acacctgaaa atatgagaaa ttgtgatcta
    25021 tttcaatttg ctcaggatct ttctttccca gcagaattgc gaatgaaaat tcactatcca
    25081 cgcttgaaag gacagggagg tattggttta aaactgaatt ggctatcgga acggttcaaa
    25141 acagagcata aggaattggt tcaaaatgat gaagaaccta gtgaacgctt attaactgtt
    25201 cgaggattag taaaacattt aaagcaacga gtagataatg ggcaacctgt cgtcgattgg
    25261 atcgcatgtt ttattgtata cggtcaaacg gttgaagaag ttcgtcaaaa tagtgcaacg
    25321 gttattcaac aacttcatac gattatccat atccaccgtg caaaaaatga tcaacgagaa
    25381 ttgttctata aatgtttatt agggcaacca ttgggacggc agttacattg gaagcaccga
    25441 acaactgtag aagcagttgg cgaatggttg ttttcagcag tatcagaatt aggtatggaa
    25501 tccggctggt atattggtcg gcaagataca ttaggggatg gtggttctcg aggagaaaaa
    25561 acaccattag aagaattaat ttatgcgtca aatcgttttg tctttttgaa tcttctagcc
    25621 attgcggaag gaatcaaagg tgcgttgttt gatgcgcctc atgtagcagt aactggaaaa
    25681 accggtaaag ggaagacctt tttagttggc ttgctctttt tgtatagttc gtttatgaat
    25741 gtacaaagtt tatttgtgga tcccaaagga gaaaagcgca aatggtttac gaaactcatt
    25801 caagacccgt attatcagaa acattaccct ttgtttgtag aacatttgca aaaaatgagt
    25861 tatgtcacgt tagatgctac aaatccccgt aattatggtg tattagatcc aattgtttgt
    25921 ttatcccgag tagatgcgaa acaagtagcc caggatatga ttgatgagtt aagtccgtta
    25981 ggtaccaatc accttttaaa aggggcggta ttaaagggaa tccaagaagt tttagttcaa
    26041 aaagaagccg gacaacaagt tggcttgatg catgtggtcg actatttaga acaaatggaa
    26101 caaaaagagg ttcgagaata tggtgagttt ttacgcttga cggtagaaga ttcgatttta
    26161 cgtttaggat tttcttatgg agaaaacccg ggattagact ttaatgccaa aacaacgatt
    26221 ttagagatcc aagatttgaa attaccaaat gacaatgtcc gaccagaact atatacagat
    26281 gcggatcgaa agtctttgtg tttgatgatt agtttaggtc gtttttgtga aatgtttgga
    26341 aaacgagata gtagtaagaa aacagcgatt tattttacag aagcatgggt atttaataat
    26401 tcgaacgcag gacgtagtat tattgcagca atggcacgtg tggggcgttc ccagatgaat
    26461 caattagtgt tagatacaca atttattggt gacttaggtt cagataaaga aaaagggaat
    26521 tttggtgtcg tctttgcttt tgatgaagac agtgagcgag aaaagatttt gaatcattta
    26581 cgtttggaag taaatgaagc gaacttagct gaaatgagcc acatgattaa aggacagtgt
    26641 tggatgttag atccctatgg acgtgtagga aaactcaatg tacacagttt gtttgaagaa
    26701 atgaccgctg cattacaaac aacggaaaaa acaagtcact ctcaagtaga agaacaggat
    26761 acgttaatca tgcaatagaa atgaggtgaa acaatggctt atataaagca taaggaaaag
    26821 aaatggtgcg ttttgttttt tttaacgatt ggattcggac tgctttttgt tggtggattg
    26881 gtgtatgcgg cagaaaagca ggtggatcaa tcgatctatg agattgggaa atttcatatt
    26941 gatacctttt cttcttattt atctgatagt gaattttcat taggcaaaat gttatttggg
    27001 gcaaatggca cagattttct aaaaatgatg acgaatatgt tctttagttt aagtaaattg
    27061 atttggcaag gccttgattt tgtcattgag aagctctatg aaggagctgc aatgaacgag
    27121 ttaattcacc agtttttcac tttttcggat actatttatt caaaaatata cgatcctatt
    27181 ggtgcgggga ttgtcgtctt gtatgtgtta tatctattta ctcttcgtat atttagaggg
    27241 aaacattacg caaaaatggt gtttattcga tttctcttcg ttgtcatttt ttcaatggtt
    27301 tggtttggct atggtagtaa cgtacctagt aaaggagaag cttttaccaa agggttaaat
    27361 gatttgtccg tggaggtaga aggctttatt ttttctgcta caaatagtct agatgaattg
    27421 gcgacaaaag atgtcaatgg tgttttagtg ccaacgacac aaaaggaagc cattcaaaaa
    27481 gtgcgccaac tttattataa agcggctgtt gttgatccgt atatattgtt aaattatggg
    27541 acaacaaatt tagaagaatt gtatcaagca gagattgaac cacaagaatt tctagccaag
    27601 gaagcgtcaa gtgaagaatt atctgatatt gagacaaaag tgaccactgc tgcaggagaa
    27661 gatagaggga aaaagaaggc ttatcgtgtc tatattcaac caactaaagc tgtttataaa
    27721 gcagtcgttg gttctatggt gccattgctt aatctaagta ttggattacc acttgtagtg
    27781 attggcatga ttcgttttat ctttcaaatg ttggtgttgt gtttattact tgtgattcct
    27841 ttttcattac tcgcttcttt tgttccagta tttgattata tgctcatgaa taccttaaag
    27901 tcctttttgg gcttgatgtt tcaaaaatct ttttacagtg tgattatttt ggtggcattt
    27961 ttgatctata acatagtaga cgatttaatt ccaatgaata ctgtttttgg ttttttagga
    28021 aatatgtttg tcaaagcact cctatcaatt ttactcattg taaaacggaa agatttatta
    28081 aagaaactag ggttaggagc tgcagatcaa actataaaca acacgaaaaa catagcaagt
    28141 cattctttgt ctcaaatgaa acaactgcct ggtcaactat caactgctgg acaaaatttg
    28201 gcaatcaaag gtgcaggatt agcagggaat gtgtatcgag caacgaatcc actcgcttct
    28261 gcagcgatac atggatttca aaagtttcaa caaaaacgta caccacaaga atcggtaaaa
    28321 gggaggcaag tggttaatcc ctttgaacta ggtgggcgtt caaatcaagc cttgccttat
    28381 agtggtcgtc atgttcgtta tcatggggta gagcctgaaa tgtcttctac caaatcgaaa
    28441 acagagtgga attctggtct ttcaaatggt ttgattccaa attcgtccat gaattcagta
    28501 agaccaaaac aacaggtaca atcattggca caattgagta agcaacaagg gaataatcaa
    28561 agaaaaccat tgttaaataa aggtatcgct tttgttggtc aatcgattcc gttgccttct
    28621 ggaaaagtac ccgtgccaca attaacagct gctggttctc tttcacaaca gattcgccaa
    28681 gtgaaaaatc aggcgaaaaa tgggagttat ccagcaatgc agcagttatt gaaccaatac
    28741 tctgttccta attcgtcggt agccactcta tcaggaaatc gttctgtgca acaaccgggg
    28801 caacgttcgt ctgaaagtaa accgtcgatt tccgcaccaa aatcgttaca acctgcattt
    28861 tcttctttag ataagccaac agagcaatcg gaaaaaatag aaaaggatcc taaaacactg
    28921 ccgattttgg acgcaaaacc aggaactgat tttactttac caaaaggacg aacagtccaa
    28981 aaaccattat cgaagaaagg atgaggaaga aatgaagaaa gggtgtctag gttgtcttct
    29041 tttctttatt cttccctttt ttctattgcc attctttttt tcaggagaac tttctgagca
    29101 tggaggagaa gctacaggtc tagctgtcgc ttttaatggg gaatataccg aaggattacc
    29161 gattttttca gagattaaag gacgtggtcc aatcacagat gaacatgcgc aagcagcagt
    29221 tggtgcagct gtaaagtatc agttattacc gagtgtgatt ctttctcaat taggctatga
    29281 atcagcttat ggacagtcac aatctggaag aaacgatacg aacttttttg ggattacctg
    29341 gtttcctggt tgtccctacc cacaaggttc tgcacgtgga attggtggtt ctgaaggtgg
    29401 tttctatatg aagtttccga acttaaaagc ttgttatgac tattatggct ttatggtggc
    29461 gagccaaagc aactttaatc aatgtgtggg caataaagat cctggaagtt gtttattgat
    29521 tctaggtcgt ggaggctatg cggcagcagg tattaccgaa gggagtccgt attatcgagg
    29581 agcaatgagt atcataacgt cctataactt aacggaatat gatacttttg cgatcaagca
    29641 ttggaatagc ctaccaacaa aaaaaacttc gcttattgca ggaaaaagta aaggcaatgc
    29701 gaaggttttg gatcggatca ttggacgatc cgtaaataat ggggaatgtt atggtggtac
    29761 ggcatggtat gtatctcaat taggtggccc acagttaatg ggttctggac acatgtttgc
    29821 gatgttaata ggggaagatt ataactggac aacctatggt tggcaagtaa tcatgaaccc
    29881 aaaaccaagt gatttacaag caggagatgt ggtgaattgg caagcaggtg gtgcattgtc
    29941 tcctggaatc tatggtcaca ccggaattat aacagatgtt tcatatggcg gacaacattt
    30001 ctctagtgtg gaacagaatg ccgagcaagg acggattatg gctcggtatc aacgcacgta
    30061 caatcaaaca aaaattcgta gtctggttcg taaggtgaaa taagaaaggc agtgactaga
    30121 aacgtgaaaa aagggataat tttagtattt agttggttat gtttgatagg tcttactggt
    30181 tgtcattggt tttcttctga aaaacaaaaa acacaattga gaacgacaga agaaacaatc
    30241 gaagcagtaa aagaaacaag tggaactctt caaagtactg aaagtaacag tcaaattggt
    30301 tcattagaag acgagacagc gcaacaaatg caaatagatg aaatgacaaa aaatttctta
    30361 aaagattttg ctagaaaatg gagtaatttc acagatgttt ataaacgcaa tcaagctgtg
    30421 agggactata tgacagatcg ttgtattaaa gacaatagta ttgatgtaga cccgcatgta
    30481 caaatgaaaa caactgggaa actttaccag gtgacacaag atatagagaa tccggatcaa
    30541 tatttgttat ttggagaaga aacaatgaat aaggttactc aatttgtatt acttcaattg
    30601 aaagtagatc aagaagctca aaagattgat gcgatcaaga tttattatgt acggagtgcg
    30661 tattaaaata aaaaatgaac actcatattt attatacacc gaagaaatag aaaaaaacag
    30721 actgtttttt taagaaaaac tatgtcattc tttacttgaa aaaagttgtt ataggacaga
    30781 aaggataaaa caaaaacgga aaaaatatgt cttatgaata actataagaa ctattctatt
    30841 ttttaggaga tggatacgag atgaaaaaac taattggcta tagtgtgatg tgtattggat
    30901 ttgttggtgt gggattgttt gtatcgactg aatctgcttc tgcggctgaa tggtataatc
    30961 ctatctggta taatccgaac attgaatgtc aaaaaggcta tccgtatgga ccgacaaatc
    31021 tgaaaaatgg aaaatgtaga gtaaattgga atggcgttgt agatagtgta attactggtt
    31081 caatggactc tgctctacat ggctttacgg gtcactagaa ggaggaagaa aatgtctgaa
    31141 gagaaaaaag aaatcgttga atcattagta gctttaaaag attctctttc taagattgaa
    31201 tatgttgata ggcaagaaat tcaaaaacta attgatgata ctattattga aatccaagat
    31261 gcgcgttgtg aaggtataaa aatttctgta gctttatcta aagtaataga aaaaatgaac
    31321 cgttcattag cgtttaatgg attgaagcta gatagacaaa ctagtctgat atgggatcat
    31381 ttaaaagact tgtatgacaa gtctaaaaga agtgaacgga ctgctgtatc aatcttaaaa
    31441 ggcctttggg gaatgaattc ataaacaaga tatttaaaaa gtaacttatt ttttatggag
    31501 gagaaattat gaaaagaata attaaaatat ttggtgtttt aggtgttttc ttattaggtt
    31561 taagtacttt tgttagtact ggaacaagtg tgtatgccac agaaaaagtg aatacagttg
    31621 atttagaaat aaagaataaa ttagtagatg aattaacttt ttattttgat acagttggtt
    31681 ttttagacga acatggtgta taccatataa aggattttgg tgctattcgt gaacgaatca
    31741 gtcaagataa cgaacaagga agagcagcaa gagtctttta tgaacatatt attgttccta
    31801 gaatggagaa agttgattgg aaacgatatg ctggatgtgt agccattaat tctcttcctg
    31861 gaggaggagt ggcttgggct ataggtacgc agggagattt tattgaagca ttaaaagctt
    31921 ttaattttga aaaagctgcg gatttattat taaaaattgc agaaaaacaa ttaggaaaag
    31981 ctgcagctaa agagttagct aaggtaaata ttgtagcagg aattgcagca gctgctgcta
    32041 gctgtgcatt agagtaaaag tttatgaaaa gtttagaaca agagcatatg gcttttaaac
    32101 aagcaatgtt taaagaaaat atctatttaa accataacta tattcgtgtt tctaaggcat
    32161 gttcaccagt attgaatatg cttggaggtg gaaatggatt atatcattta ctgtttgtcg
    32221 atgtttgttg gttaattttt ttaccagatg aactagttat agtcaatgaa a
//
LOCUS       DAZKCY010000022        31295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000022 DAZKCY010000000
VERSION     DAZKCY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.022"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..445
                     /locus_tag="ACG8XC_000921"
     CDS             53..445
                     /locus_tag="ACG8XC_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9190636.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            473..1813
                     /gene="glnA"
                     /locus_tag="ACG8XC_000922"
     CDS             473..1813
                     /gene="glnA"
                     /locus_tag="ACG8XC_000922"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFP9190637.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2539..2784
                     /locus_tag="ACG8XC_000923"
     CDS             2539..2784
                     /locus_tag="ACG8XC_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190638.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3006..4616
                     /locus_tag="ACG8XC_000924"
     CDS             3006..4616
                     /locus_tag="ACG8XC_000924"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFP9190639.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4593..4805)
                     /locus_tag="ACG8XC_000925"
     CDS             complement(4593..4805)
                     /locus_tag="ACG8XC_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190640.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            4908..5777
                     /locus_tag="ACG8XC_000926"
     CDS             4908..5777
                     /locus_tag="ACG8XC_000926"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP9190641.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            5942..7213
                     /locus_tag="ACG8XC_000927"
     CDS             5942..7213
                     /locus_tag="ACG8XC_000927"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9190642.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7210..8505
                     /gene="rho"
                     /locus_tag="ACG8XC_000928"
     CDS             7210..8505
                     /gene="rho"
                     /locus_tag="ACG8XC_000928"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP9190643.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8604..8867
                     /locus_tag="ACG8XC_000929"
     CDS             8604..8867
                     /locus_tag="ACG8XC_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9190644.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9312..9761
                     /locus_tag="ACG8XC_000930"
     CDS             9312..9761
                     /locus_tag="ACG8XC_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9190645.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9818..11236
                     /locus_tag="ACG8XC_000931"
     CDS             9818..11236
                     /locus_tag="ACG8XC_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9190646.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11236..12096
                     /locus_tag="ACG8XC_000932"
     CDS             11236..12096
                     /locus_tag="ACG8XC_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9190647.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12270..13043
                     /locus_tag="ACG8XC_000933"
     CDS             12270..13043
                     /locus_tag="ACG8XC_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9190648.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13190..13555
                     /locus_tag="ACG8XC_000934"
     CDS             13190..13555
                     /locus_tag="ACG8XC_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFP9190649.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13703..14911)
                     /locus_tag="ACG8XC_000935"
     CDS             complement(13703..14911)
                     /locus_tag="ACG8XC_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9190650.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(14930..15208)
                     /locus_tag="ACG8XC_000936"
     CDS             complement(14930..15208)
                     /locus_tag="ACG8XC_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190651.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15401..16057
                     /locus_tag="ACG8XC_000937"
     CDS             15401..16057
                     /locus_tag="ACG8XC_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9190652.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16499..17296)
                     /locus_tag="ACG8XC_000938"
     CDS             complement(16499..17296)
                     /locus_tag="ACG8XC_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFP9190653.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17293..18477)
                     /locus_tag="ACG8XC_000939"
     CDS             complement(17293..18477)
                     /locus_tag="ACG8XC_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFP9190654.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18502..19749)
                     /locus_tag="ACG8XC_000940"
     CDS             complement(18502..19749)
                     /locus_tag="ACG8XC_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9190655.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            19964..21709
                     /gene="dnaX"
                     /locus_tag="ACG8XC_000941"
     CDS             19964..21709
                     /gene="dnaX"
                     /locus_tag="ACG8XC_000941"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP9190656.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21729..22043
                     /locus_tag="ACG8XC_000942"
     CDS             21729..22043
                     /locus_tag="ACG8XC_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP9190657.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22068..22664
                     /gene="recR"
                     /locus_tag="ACG8XC_000943"
     CDS             22068..22664
                     /gene="recR"
                     /locus_tag="ACG8XC_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP9190658.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22728..23372
                     /gene="tmk"
                     /locus_tag="ACG8XC_000944"
     CDS             22728..23372
                     /gene="tmk"
                     /locus_tag="ACG8XC_000944"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9190659.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23387..23716
                     /locus_tag="ACG8XC_000945"
     CDS             23387..23716
                     /locus_tag="ACG8XC_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFP9190660.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23730..24668
                     /gene="holB"
                     /locus_tag="ACG8XC_000946"
     CDS             23730..24668
                     /gene="holB"
                     /locus_tag="ACG8XC_000946"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP9190661.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24704..25528
                     /locus_tag="ACG8XC_000947"
     CDS             24704..25528
                     /locus_tag="ACG8XC_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFP9190662.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25521..25868
                     /locus_tag="ACG8XC_000948"
     CDS             25521..25868
                     /locus_tag="ACG8XC_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFP9190663.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            25937..26809
                     /gene="rsmI"
                     /locus_tag="ACG8XC_000949"
     CDS             25937..26809
                     /gene="rsmI"
                     /locus_tag="ACG8XC_000949"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP9190664.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            26918..28039
                     /gene="dinB"
                     /locus_tag="ACG8XC_000950"
     CDS             26918..28039
                     /gene="dinB"
                     /locus_tag="ACG8XC_000950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP9190665.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28093..28695)
                     /gene="nrdG"
                     /locus_tag="ACG8XC_000951"
     CDS             complement(28093..28695)
                     /gene="nrdG"
                     /locus_tag="ACG8XC_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFP9190666.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29010..31199)
                     /gene="nrdD"
                     /locus_tag="ACG8XC_000952"
     CDS             complement(29010..31199)
                     /gene="nrdD"
                     /locus_tag="ACG8XC_000952"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP9190667.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT        10042 a         5618 c         6614 g         9021 t
ORIGIN      
        1 tttttttaat tggtacactt tagccaactt ataaaacgaa aggggacgtc tgatgggaga
       61 aaaggaattg cgccgatcta tgtccgtatt tccaattggt acagttatga agctgactga
      121 tttgtctgct cggcaaattc gctattatga agaacaagat ttagttcatc cggaacgaag
      181 cgaaggaaac cgacgtatgt attcgctgaa tgatattgat atgttgcttg agattaaaga
      241 ctatctttca gagggattga atatggctgg gatcaaacgt gtatacgaaa tggcacaggc
      301 gaaaaaacaa gaagcaaaaa acaagaagcc gttgacagat catgacgttc gacaaatttt
      361 tcgtgatgag attttggcgc aaggtgggtt aagcaaaaca ggtcattatc agtcgcaagg
      421 attgcaaaga caattctttg actgaataaa ttaaaaaagg aagagggata ccatggtaaa
      481 gaaacagata acaggtgaag aaatcaaacg aatcattgag gaagaaaacg tacgattttt
      541 acgattgatg ttcacagaca tcttaggtac gatcaaaaat gtagaagttc cagtgagcca
      601 gatcgacaaa gtattagaaa ataaaatgat gtttgacggt tcttcgattg aaggatttgt
      661 tcgaattgaa gaaagcgata tgtatttgta tccagatctc tccacttgga tgatcttccc
      721 gtgggaaagt gctcatggaa aagttgctcg tttaatttgt gatatctata atccggatgg
      781 gacaccattt gccggcgatc ctcgcgggaa tttgaaacga gcattgaaag atatgcgcga
      841 ccttggtttt acctctttca atttaggacc tgaaccagaa ttcttcttat ttaaattaga
      901 cgaagacggt gggatcacaa cgaccttgaa tgataaaggt ggttattttg attttgcacc
      961 aacagattta ggtgaaaatt gccgccgtga catcgtgttg gaattagaaa gtctaggctt
     1021 tgaagtggaa gcttcacatc atgaagtagc gccaggacag catgaaattg actttaaata
     1081 tgcagatgtg gtagatgctt gtgataatat ccaaaccttc aagctcgttg tcaaaacgat
     1141 tgcacgcaag catggacttc acgcgacttt catgccgaaa ccgttatttg ggatcaatgg
     1201 ttctggtatg cattgcaata tgtctttgtt caatgatgaa ggaaatgttt tctatgataa
     1261 agacggtgaa ttggaactaa gtgaaacagc ttatcatttc ttaggtggtt tgctgaaaca
     1321 tgctcgtgcc tacacagccg tttgcaatcc aacagtcaat tcttataaac gtctcgttcc
     1381 aggttatgaa gcgcctgttt atgtcgcatg gagtggccgt aatcgttcgc cattagtacg
     1441 tgtaccagaa tctcgcggtt tatccacacg tttggaattg cggtcagttg atccatctgc
     1501 aaacccatac ttggcaatgg cagtattgtt gcaagcagga ctagacggta tccgtaatca
     1561 gatcacaccg ccagcagctg ttgaccgtaa catttatgta atggatgagg aagaaagaga
     1621 agctgcacat atccaggact taccttcaac tatccataat gctatcaaag aattgcgtaa
     1681 ggataatgta atgattgacg cattaggaca acatatcttc tcaaattttg ttgaagcaaa
     1741 acgtctcgaa tgggcggcat tcagacaaac tgtttccgag tgggaaagag aacaatatct
     1801 agaactttat taaaacagga ataccgtgaa ttagaggctg tgaaatcagc gttttgacct
     1861 gagtattaga gaaactaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat
     1921 accgagggtc agctgattga acaccgttta gtggagaggt tgtgaaaaag ctgccctgca
     1981 acaagtaata agaatagcca agcaaaaaca gcttttcttg tttttgaatt ggctgaactt
     2041 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttttgaaa aagacgttta
     2101 gtttcgagta ttaagaaaaa ttttgtgaaa atggctttct acatttttgc ataaatttgg
     2161 cttaataccg agaaactggc ttttgaatac cgtttatcca gaggttgtga gcctaccatt
     2221 taactccgaa tcacaagaaa taatttcaaa aacagcttct catgtttgat ggatattgtg
     2281 acttgtgacc gaggagttgg caggagaaca ccgtttattt agaaataagg aggcacgact
     2341 tttgtcgcat ctccttattt tttacttatg tgattcgtta ccttcaatga atccctacaa
     2401 ggcacttttg ttgaaaaaaa ggtcatcttt gaaaaaatta gtggaattgt tttgtttttg
     2461 atattattta atagaaaaga tatgccaaaa atccgagaac tgtggactac gttttttacg
     2521 taaaggaggt aaataagggt gaaagaaaaa gattgtttct acgataagct atataaactg
     2581 ttgctgcaag taggaaaaag tagttctttg ttaggtggga tattatctat cataatgttt
     2641 gttttatttt tttatctatt atttcactta aaatttttca gtaacgattt tctgatttgc
     2701 ttaggtttaa cgttagctat gtttatatca agcttattat gtgggttaaa ttttttttgc
     2761 actcataaag gacttaagaa ataatttctc aaaggatatc gaaaaagaaa aaattgtaag
     2821 cggatagtat tcatttgagt acttgacttt cccttagtca gcttgtatta tacaccttgg
     2881 gcaccctttt agagggtcag aagtttacag aaatttcggc tccctattct tattgaagaa
     2941 tagggagctt tgtttattgt ctgagacttt tcctgcacat aaattaaaaa ggagtttttg
     3001 aacttatgac aaaatatatt tttgtgaccg gcggtgtcgt ttcatcaatc ggaaaaggga
     3061 ttgttgcagc ttctttagga cgcttattga aaaaccgcgg cttaaaagta actatccaaa
     3121 aatttgatcc atacatcaac gtggatccgg ggacgatgag cccatatcag cacggagaag
     3181 tattcgtaac agatgatggt gctgaaacag atttggatct gggccactac gaacgtttta
     3241 ttgacatcaa tttgaacaaa tactccaacg tgacgacagg aaaaatctat tctgaagttt
     3301 tgcgtaaaga acgtaagggc gaatacttgg gagcaactgt tcaagttatt cctcatatta
     3361 caaatgagat caaagaaaaa atcatgcgag cagctaaaat gactgattca gatgtgatca
     3421 ttacagaagt agggggaaca gtaggggata tagaatcttt gccattcctt gaagcacttc
     3481 gtcaaatgaa agcagatgtc ggtacagata atgttatgta tatccataca acattgattc
     3541 cttacttgaa agctgctgga gaaatgaaaa ctaaaccaac gcagcacagt gtaaaagagc
     3601 tacgtggttt aggtatccaa ccgaatattt tggtggtacg tacagagcaa cctgtttctc
     3661 aaagcgtgaa aaacaaattg gcacaatttt gtgatgtcga accagaagca gttatcgagt
     3721 cgccagatgt agatacactt tactccatcc cattagtttt acaagcacaa ggaatggatc
     3781 aaattgtttg cgaccattta aaactagata caccagaagc agacatgaca gaatggaaag
     3841 aattagaaga acgtgtcttg aacttgaaga aaaaagtacg gattgcgctt gtcggtaaat
     3901 atgtcgagtt accagatgca tatatctcag tagttgaagc attgaaacat tctggtttcg
     3961 cctataattc agacatcgag atcgattgga tcaaagcaca agaattaacg agagaaaatg
     4021 tagaagaacg tttgaaagac gcagatggaa tcttggttcc tggtggattt ggtgatcgcg
     4081 gagtagaagg gaaaatcgaa gcgattcgtt atgctcgcga aaatgacgtt ccgtttttag
     4141 gtatttgtct tggtatgcag atggcttgtg tcgaatttgc acgtaacgtc gttggattag
     4201 aagatgcgtc atcagctgaa acgaatccag atacagcaaa caatattatt gatttaatgg
     4261 ctgatcagga aaacatcgag aatttgggtg gaactttacg ccttggatta tacccatgta
     4321 aaatcaaaaa aggaacgaag acggctgaag catatgatgg agccgatgtt gtgcaagaac
     4381 gtcaccgtca ccgctatgag ttcaataaca aatatcgtca attgtttgaa gaacaagggc
     4441 tagtcttttc aggtgtctca ccagataacc gtttagtaga aatcgtagag ctgccagaga
     4501 aaaaattctt tgtgggatgc caattccacc cagaattgat ttctcgtcca aatcgcccgc
     4561 aaaaattaat caaagcattt gtcggagcat cattagcaga tcacgaaaca aaataatttt
     4621 tgaacttgat agaaagagaa aagtagcaag agaaggggat aagcaatttt cttgtcactt
     4681 ttctcttttt ctgatttgtt attatttgta ggaaaaaaga tagaaagcaa aagacttttt
     4741 tcgattaaag cgctattgga tattttattt tttgcaaaaa acttcttgaa aattttcatt
     4801 ttcattgatt ttagtagaaa actaccgaaa tgtttggtaa aatagggtcg ttggttaaga
     4861 attgtcttaa caaatttagt tttacaaaac ttaggaggaa ttttattatg ccagtagtat
     4921 caggagctga atttttaaaa gctgctcgta aaggcggtta cgctgtcggc ggattcaaca
     4981 caaacaactt ggaatggact caagcaatcc ttgaagcggc tgaagcaaaa caagcaccag
     5041 tacttatcca aacatcaatg ggtgcagcta aatacatggg tggttacaaa atctgtaaag
     5101 atttaatcac tgatttagtt gaagcaatga acatcactgt tccagttgct atccacttag
     5161 accatggtga ttacgaagct gctttggaat gtatcgaatt aggctatact tcagtaatgt
     5221 tcgatggttc acatctacca tttgaagaaa acttgaaatt agctaaagac gttgttgaaa
     5281 aagcacatgc aaaaggtatc tcagttgaat gtgaagttgg ttcaatcggc ggagaagaag
     5341 acggaatcat cggtaacggc gaattagctg atattgaaga atgcaaacaa atggttgcta
     5401 caggtatcga ctacttagct tgcggtatcg gtaacatcca tggtcaatat ccagaaaact
     5461 ggaaaggatt agcttttgac cacttacaag caattgctga tgctgtaggt ccagacatgc
     5521 ctttagtatt acatggcgga tcaggtattc ctcaagatca aatcgaaaaa gctatctcaa
     5581 tgggtatctc aaaagtaaat gttaacactg aattccaatt atcatttgct gcagctactc
     5641 gtaaatatat cgaagctggt aaagataaag aaggtaaagg attcgaccct cgtaaattat
     5701 tagctccagg taaagcagca atcgttgaag atgctaaatc tcatatcgac tggttcggtt
     5761 cagctaacaa agcgtaagca tccacataac agatacgata tataggaatg gagaacgact
     5821 tttgaagtcg ctctccattc ttttttactt aaaacggcag accatgctcg tttctttttt
     5881 ttatctatga taaaatacaa gcatatccat gaaaaaagat taataaacaa ggtggaaaca
     5941 gatgaaaaaa atcgtaatca aaggaaaccg acctttagct ggtgaagtaa cgatcagcgg
     6001 tgcaaaaaat agtgcagtcg cattgattcc agctgcaatc ttagctgatt ccccagtgat
     6061 cttggaaggt gtgccggaca tacaagatgt tcattcatta attgaaattt tagagatcat
     6121 gggcgcaact gtccattttt ccaacaacat actagaaatc gatcctcgag aagtcgtatc
     6181 gattccgatg ccgcacggaa aaatcaatag tttgcgtgct tcttattatt ttatgggaac
     6241 actgcttggt aaatttggcg aagcggtagt aggcctgcca ggtggctgtt atttaggccc
     6301 tagaccgatt gacctacacg tcaaaggatt tgaagctcta ggtgctgaag tgacaaatga
     6361 acatggtgcc atgtatttac ggacagaaaa taaaggattg cgtggcaatc ggattttcat
     6421 ggacgtggtt tcagtcggag caacgatcaa tgtgatgtta gctgctgtaa aagcaaaagg
     6481 acagacagta attgaaaacg ctgcacgaga accggaaatc atagatgtgg caactttact
     6541 gaataatatg ggggcaaaag ttcgaggagc gggtacagat gtgatccgta tcgaaggagt
     6601 cgaaacatta catggctgtc ggcatttcat gattcctgac cgcatcgaag caggaaccta
     6661 tctagcatta gcagctgcag taggaaatgg tatcaaagtg aaaaatgtga tctttgaaca
     6721 tttggaaagc tttatagcaa aattgcagga aattggtgtc catatgaaaa tcagcgaaga
     6781 tgaaatcgaa gtttacccct cgaaagaatt aaaacccgca aacatcatga cctatcctta
     6841 tccaggattt gccacagatc tgcagcagcc acttactgcg ttactattga tgactacagg
     6901 cacttcagaa atcatcgata cgatctatgc gaaacgtgtg aaccatgtgc cggaactagc
     6961 acgaatgggc gctgatatta ccgtggaagg aaatatgatc atcgtgaatg gtccaaataa
     7021 acttcacggc acagaagtcg tggcttctga tcttcgggca ggtgcttgtt tagtgattgc
     7081 tggactattg gcagaaggga caacgacgat ttacaatgta gattatatct tacgcggtta
     7141 tgatcatatt atcgagaaac taacagcctt aggggcatca attgaaatga tagaggaagt
     7201 gggagctgaa tgagcgatta tttgacaatg gcagaactag agaacaaaac gttaaaagaa
     7261 gtttatagtt atgccaaaga atttaaaatt ccttattaca gtaagatgaa taaaaaggaa
     7321 ttatctctag ctgtgattcg tgcacaagca gagaaacaag gattttatta tatggaagga
     7381 atattggata tcgttggtca agatggttac ggttttttac ggccaatcaa ttatggacca
     7441 agtgcagaag atatctatat ttctgcctca caaatcagac gattcagttt gagaaatggg
     7501 gacaaagtgg caggaaaagc ccgtccacct aaagaatcgg aacgttatta tggtttgatg
     7561 cacgtggaaa gcgttaatgg aaaagatccg gaagaagcaa aagaacgtcc gcattttcca
     7621 gccttaacac ctctttatcc gcaaagacag cttcaactgg aaacaacacc gcaaagacta
     7681 tctacccgaa tgatcgatat cttttctcca atcggttttg gtcagcgtgg attgatcgtc
     7741 gcaccgccaa aagcagggaa aacttctgta ttaaaagaaa tcgcaaatgg tatcaccgaa
     7801 aactatccgg aagtagaatt aatcgttcta ctgattgatg aacgaccgga agaagtaacg
     7861 gatctagaac gaagcgtgaa aggcgacgtg gtatcttcta catttgatct gcagccgcaa
     7921 aaccatactc gagtttctga attagtattg gaacgcgcaa tgcgtttagt cgaagataaa
     7981 cgagatgtcg ttatcttgat ggatagtatt actagacttg ctcgggccta taacttagtc
     8041 gttccaccaa gcggacgtac attgagtggc gggattgacc cagccgcatt gtataaacct
     8101 aagaaatttt tcggggcagc aagaaacatc gaagaaggtg gcagtttgac gattttagcg
     8161 accgctttag tcgatacagg aagccgtatg gatgacgtga tctatgaaga attcaaagga
     8221 actggaaatc tagaattaca actttcacgt gaattatctg aacgtcggat cttcccagcc
     8281 atcgatatca aaaaatcagg tacccgaaaa gaagaattat tgatgccaag tgaagaatta
     8341 gaagaaattt ggaaacttcg aaaaaatatg actggagatt cattagaata tacggaacaa
     8401 ctgatccgct ttttgcgtaa aacaaaaaac aatcagcaat ttttcaaaga atttcaagat
     8461 gtctcctttg gtaaaaaaaa tccgaccaga aatctgaaaa gataattgca accttgactg
     8521 aaacatgtta agatgttaat gttgtaaatc gacaaatcaa ctctggttca gcaaatggag
     8581 cagggcaaag gagcgaaaaa atcatgaaag aaaacatcca tccagattac cacccagtag
     8641 tattcatgga ttcaacaact ggtttcaaat tcttgtcagg ttcaacaaaa acttcacaag
     8701 aaacagttga atgggaagac ggtaatacat acccagtcat ccgtgtcgaa gtaacttctg
     8761 actcacatcc attctacact ggacgtcaaa aattcacaca agcagacgga cgtgtggacc
     8821 gtttcaacaa aaaatacggt ctcaaagacg caaacgctgc ggaataataa aaacgctgat
     8881 aactcaacgt ttagcagact atctgatagg gatagtctgt ttttttatgt tttttgccaa
     8941 tttaaaactt cgtttgatgc tgaaaaagtc gtaggatcat aatgcacaaa acaatgaaaa
     9001 actagtatat agttttaatt atccacttga taaaaacacc ttatattaac gaaatgaaga
     9061 ggggttattt cgaagtaaaa aaccatttac ctaaaattag aagctaagct ttcctttcaa
     9121 ggaaggcttt ttgttttgtt atagacttgc gattgtttgt gagctagatc gttcatttta
     9181 atgacttata cacgcggatg atgggtcttt ttcctctgtt cattcataat attgaacggt
     9241 acagctagcg atgctatact gacctcagtt ccaagaaaac gttaacattt tttgtggggg
     9301 agaggttttt tatgaggttt gatcaattga tcgcagaaga actgattgtt ttcgatccat
     9361 cgatagattc aaaaggatca ttatttgagc gaatagggga tgtgttgaaa aaaacaaata
     9421 aggtaagaaa tgttcaaaaa gtcgttaagg cactattcaa acgagaggag gagatctcca
     9481 caggaataga agatggattt gggattcccc atgcaaaaag caaatatgtg acaaaaccag
     9541 cactgtgttt ttttcataca gccaaattaa cggactatag cgggatagat gaaatgccaa
     9601 ttgaatatat ttttgccatc gtagttcctg aaaaatcagc agatatgcat ctagagattc
     9661 tcagcacact ctccagacga ttgatgaatg cagattttag aaagggaata aaagaagcca
     9721 ctacgccagc agatatcatc agacagatta gtcaagaatg aaaatagtag agtctttgtc
     9781 cgagtatcga tagtgaagaa aaaggaggaa aataatcatg aaaatcgttg gtgtaactgc
     9841 ttgcccgact ggaattgctc atacttatat gtcggcagaa aaattagaag taacagcaag
     9901 aaatctaaac catcaagcaa aattcgaaac acaaggtgta aaaacagaaa atcagttatc
     9961 tgaacaagaa atcaaagagg cagatgcaat cattcttgca gtagataaag aaatcgaatt
    10021 ggaccgattt gccgggaaaa aagtaaagcg tgtctctaca agccgagcaa tcaaagaacc
    10081 gcaagtagta atagacgaag cattgagaga tattggcgta ttcgttgtgt ctaatgaaaa
    10141 agaaccagct gcaaatgaga aaaagccgac aatctataat catttcatga atggtgtgaa
    10201 ttacatgctt ccatttgtga tcgcaggcgg aatcatcatc gcactgagtt ttgcctttgg
    10261 gattacagcg gctgatccca actcagcaga ctataatgtg ttagcagctg cattttcaag
    10321 gatcggagga gatacagcgt ttgccatgat ggttcctgcg ttaggcgcgg ggattgctac
    10381 atcgattgct ggaaaagcag gttttgcccc aggaattgtt gccggattac tggcctctac
    10441 aggtggttca ggttttcttg gcggaatgat tggtggcgtg ctagcagggt atatctgtga
    10501 ttttctggca aataaaatta gcgtaaaaaa agaattttct gctatttatc agctgattgt
    10561 cgtccccttt ttatcgatct taattattgg gcttttgatg gtatttgtga ttgaacaacc
    10621 gatcacatgg gtgttggatg ctttgacaaa ttggctgaat agcttgggaa atacatccgg
    10681 gctattgttc ggattactta tcgggatcat gatggcagct gatatgggtg ggccgatcaa
    10741 taaatcgact tcgactttct cgatcggttt gatgtcagct ggagtaaatg caccgattgc
    10801 tgcttgtatg gctgcaggaa tgacacctcc cctaggtatt gctttagcca ctttattatt
    10861 caaacataaa tttaccaaag aagaacggac ttcaggtcaa tcatgctgga ttttaggcgc
    10921 ttcgtatatt acagaaggtg ctataccgtt tgcggtatct gacccgatcc gcgtgatacc
    10981 tagtttgatg acaggttcag cagtcgctgc agggatttcc atggcagtag gagttacttc
    11041 aatggcacct catggaggga tttgggtgat gctaattcca aatgttatga acaatttacc
    11101 tatgtatgtc ttggcaattg catcaggtac agtcactaca gcctttatgc ttggactatt
    11161 gaaaaaaaca gtgaaagaga aaaaacaaga caagtggacg aaaaatgaaa atctaacaaa
    11221 tgaggtggaa gcgtaatgtt atatacaatg aaagagttat tgaaagtggc taaagagaat
    11281 caatttgcag taccagcctt caatatttgc agctatgata tgatgaaagc aatcatcgaa
    11341 gaagcagaga gattagaagc accagtaatc ttagaaattc atcctgatga aatcgcctat
    11401 ttaagtgatg agtttgttgc ggcagtaaga gcctatgctt atacaagtaa actgccgatc
    11461 gttatccact tggatcatgg cggtacgata gaagatgtct tacgggcgat tcgaaacggc
    11521 tattcttccg tgatgatcga tgcgtctttg aaatcattcg acgacaatgt acagattacg
    11581 aaaaaagtgg ttgcgttagc tcatgcggta aatgtctcag tggaggctga gcttggaacg
    11641 attgggaata atggttctgc tgaaggaggt gctgaaacaa tcatttatac agatcctgat
    11701 caagcgaaga tttttgtgaa agaaacagga atcgatacac ttgctgtagc aattggaaca
    11761 gcgcatggct tgtatccgaa aaacagaact ccaaaattaa atatcgactt actgaaagaa
    11821 ttgaaccaac aaatcgatct tccatttgtt ttacatggcg gttcaggtaa tccggacgca
    11881 gaagtcagcg aagcagtaaa atacggagtt gccaaagtga atttgagctc agatcttaaa
    11941 agcgtctttt tcaatgcttt gagagaaata ttgagtgaaa atccatcgat gtacgagccg
    12001 aatgcaattt atccagcagc taataaaaag gtacaagaag tggtcaaaca taaaatggaa
    12061 attttgaaca cagtaggcaa ggctagttta tactaaaaat aaaaatgtgt ggtcaagagg
    12121 gtatgacaat agtcttgtca taccctctta accacacacg atgttcagaa gtagtttttt
    12181 cgaaaaattt cttcttattt ctcgaagcca atcacttctg tcacaacctc ttttttcctg
    12241 ttactatata aagtaaatgg ggtgatttta tgttgaatga agtggagaat agacagcagg
    12301 agattgtgaa ttatctaaaa aacaaccaat ttgcaagagt caccgatttg atcgagttag
    12361 taaattatag cgaagccacg attaaacgtg accttgtttt tctggaaaaa agtggattga
    12421 tccgtagagt tcgcggtggc gccatgctta ttgacaatcg aaaaattgac gtgccttacc
    12481 tgatgaagat caccaaatta gaagaagaaa cagaaaaacg taagattgca gatacagctt
    12541 cttctttgat tcgagatgat atggtcttat tcttggattc cagtacaacc tctttacact
    12601 tagtcagaaa cctagcaaga tttgatggtt tgcagatcat tacgaatggc atcgtcacag
    12661 ctgctatgtt gagtgaatat acaacggcaa aagtatctgt attgggaggg gcaattgttc
    12721 cgaaacgagc aacaatcaac ggttcaaaag cctataacga tgcattgtca tattatgcgg
    12781 atttagcgat aatgagttgt cgcgggctag atttcagcca aggagtaaca gaaactcatg
    12841 aaggagaggc aatggtcaaa aaagctttta gaaaacaatc agaccggctt gccgttttag
    12901 ttacacagca aaaaattgca cataaattca tgcatcaagc cattgctttt catgaaatcg
    12961 attatctggt gacaagtaaa gaactgacaa aagaacagta tgcgaaagta aaggaacacc
    13021 atatcgaatg tctgtacgga taaattatta cgtgaatgtt ctaagtggct tcctcatttt
    13081 tagataggac cttatctttg ctcgcttctc tcagccgatg gtataataat gaaaaggtta
    13141 tcttgttaga taatcttact tttttatcca ggagaaagga gtgcagaaaa tgaaacatgt
    13201 gaaggcgttg gtagtgaagg caatcatgat ctgggcgatt ctgtgggtcg tgctgacagg
    13261 tttatacggt gttagcttta tggattcaac gattgttggg gtaattattg tagtcatgat
    13321 ctatgtgcta ggggatctgt tgatcttacg taaagtaggg aacatcgcag cgacgatcgc
    13381 agatgctggt tcagctgcag ttatcttgtg gctctatcta tattttatga atgatacagt
    13441 cgatatttgg atgaaagtat tgattcctgc tctattgatc ggtgtggcag aatggttctt
    13501 ccacaagtgg ttgctaagtc aaggcatcgt accagatgaa cggaaaatgg aataaatcaa
    13561 cagttaaact attcttatcg ataaatgaaa gctttaaaaa taaatagatg tttaattaag
    13621 aataatcata aactattttt actcaaaaaa ccgctcagat tattttcaga tctgaacggt
    13681 tttttttatc tattcatcaa gatcaaattt catttttcaa tgattcaatc aattcgccct
    13741 ctgcttcatg actatgtttt cggccattaa caagatagag tgctgtacat atcaatggaa
    13801 tgactccggc aatcacatac aatcctctaa atgaagtaag agattttagc atccccatca
    13861 aataaggacc aacgcctaag cctaggtcta aaccaataaa gtaggtagat aaagcaatac
    13921 tgattcgatg ttcgcggacg atttttaaac aaacagcctg tccatttgac ataaacgtac
    13981 cataacctag gccgactaat gctccagaga taagcaatgt cacactattc gtcgttacac
    14041 tcagtaaaaa taatcctgct gttaagaata aatagctagg atacatcacg tattgttctc
    14101 ctttgacatc gaaaatcctt ccagtcaatg gacgtgtaaa tgtgatgacc aaagcatata
    14161 cgacgaagaa aaatgaccca gcagcaacta agtggattac ttttgtataa gaagataaaa
    14221 atgataaaac acttgaataa gaaagaccca tcaaaaaggc aattcctgta atgaaaagtg
    14281 ctttcttttc aataaaacta ttgatattcc atgagcgaag agatattttt tgttcttcag
    14341 ataatggaat attttttaca ggaaagacaa ggcaggcaat cgtaacaaag aagaccagta
    14401 caattgaaaa ccagataatg aaacgaaaat cagtcatatt cagcaaaatc attcctaaaa
    14461 atggcccaac agcggcagct aaactagtac ttaatccata ataattgatg ccttcccctt
    14521 ttttagatgc agggatatat gcagtaacga tagcatttgt agctgtagaa actgtaccgt
    14581 agccgaatcc attcaagaat cgaactaaat acaatgcccc gatagttggc gtataaagat
    14641 atgctaatgt tgaaaggaga tagaagatcg cgccgcctct taaggtaaac ttccgaccaa
    14701 atagttctaa ctgtttcccc atgaataagc gtgccagcaa tgttccgatg atatagatcc
    14761 cagaagcaaa acctgcttgg ctcaagctgg cgtgtaaggt gtcttgcgca atgactgcca
    14821 tgatgaccat caacagataa taaatcaggt agacaataaa attgatcaat gtgattccaa
    14881 taaaatattt attaaataat ttcttttcca tgtatgttat acattcccct taagctgaat
    14941 atgcttttaa tttttgtatt tgaacttcta catcgtgata attggatgga tagcgatcaa
    15001 caagttggcg aataaataaa tctcttgtag acataaacag atttaaaaat tcttcgcatt
    15061 cgtttgttgg attctcatcc actgctttga tcaattcgat cgagttttga tacgcatcat
    15121 ttaaaaagac taaagaagct ttcatatcaa atcttgtttg atatgctgtt tctaatcgat
    15181 taatgattgt gttcatccag taattcatgt tttttccctc tattcttaaa aaattttact
    15241 tctgcagtat aacgaataaa aaaaaggaaa aaaatcgcat aagtgatgga aaaaggtact
    15301 taacaaatgt taatatcatt agtaaaatac ctttggactt taaaaagttt taaagtactg
    15361 tatgatcttt ttagatgaaa gtttggatgg gggagcatca ttggatagta gagtcttaaa
    15421 ggaatatttg gataaaaatg attttccgat tatacgcaaa ggatatcata aatatttgac
    15481 attcgaaggg atggaagatt cccatacgta tatcttgaaa gagggaacgg taaaagcaag
    15541 tgttatttca aatgatggga gagaatttaa ccttcgctat atcaaagatc ttgaaatcgt
    15601 ctctctttta aaagacgaat attcacaatt catcgattcg ccgtataata ttcgagttga
    15661 atcagaatat gcagagtttt atcgaatcaa tcgtgtgcgc ttttggcaaa atatcaacca
    15721 agacccaaag ctacaacact atgtaaaaga gtattaccgc tatcgtctgc tttattcgat
    15781 gaaaaaaatg cagcaaatgc tattaaacgg aaagctggga gctgtttgta cgcaacttta
    15841 cgaattatat gatttattcg gtgtgcggac agaagaaggg atgctgatcg atttttcgat
    15901 cacgaatgaa gaaatcgcca aattttgtgg catctcaact gctagcagtg taaacagaat
    15961 tttaaaacaa ctgaaaaaag atggtattat caccgttaaa gaccatcata tcgttatttt
    16021 gaatatggac ttgttagaag acaatattat cttttgatga acaaagaggt tgtgaaatca
    16081 gcgttttgac ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    16141 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct
    16201 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    16261 tattgtgact tgtgaccgag gagttggtag gagaacaccg tttaccaaga ggttgtgaaa
    16321 aagctgttct gcatcaagta ataagaagag ccaagtaaaa acagtctctt atgtttttaa
    16381 cttggctgga cttatcaccg tagatgtaag cttttgaacg ccgtttattt gcaacaaaag
    16441 gggctgtgac acgatttttg tcacagccct ttttttgcga acattcattt agcaattttt
    16501 agaaacttct gccgccaccg ccaaaagtcc cgccaccgct agaatgggta gtacttccgc
    16561 caccgccgct tccaggaggt gggcttttgg ggattcttct tgtcgtgaca aaagaatttg
    16621 tcagacgatc ggttttttca gttaatttga tactggattt ttctcggaat ggatatttgt
    16681 agatgcctga tttcaattga taacgcgaaa cagtcacaaa ataaaagaca agactaagga
    16741 taagagccaa aacaacagca atgatgattt ctactgtcgt caaaacctta tagcgtgtaa
    16801 ttttgccagt ttcttcgtct acacgataat gtcctcctgg aactccgtct tccacatatg
    16861 ttgctgattt agacaggtaa gcttttgcag cattgtaata ggcagcattt gtcatctgat
    16921 cataaacatc atctaagatc gaatcgattc tgctatcagt caaataatcg atcatattac
    16981 cagaagtcga aatgtagatt tcacgctgtc ccatatctaa tagcaatact gagccatttt
    17041 gatcatttcc gactgcgtct cttaagtaat tatcagcaaa gtctcttggt tcttctgaat
    17101 tagatgtagt cgttacgatc aagactcgtc ctttgatttt ttcattcagc ttttctgcct
    17161 gttgttccaa agattggatt tgatctgttg tgaataaccc agcctcatca tttaccgtag
    17221 ctgtatctgc atgagtagcc accggccagc caacacttac tagcaaaaga aagaataaaa
    17281 aaaatttttt catcatatca agtaacctcc gatcagaaag aggatcgcta gcccagcgaa
    17341 aatcccaaga gcggtcagtc ccaatttttt ctggctgatc ggcaggacac cgctgacctt
    17401 tcctgtttgt ccattcattg cgtagtagta gactttttta cttgagtcat tgcttcgata
    17461 agtaacaagc cataccggca gtaatacgta attatttact tcgtttgtga ttgaagcaga
    17521 agttcgcaca ccggtcaatg ttgtatatcc atttgcggta tcttttaaca gcttctctga
    17581 atagtctttt aattcagatt gaatctgtga tttgattgcc tcatattcga tatctctttt
    17641 ttctgcttgg aacccggcta aatactggct tttaaaatca attgctttat ctaacgggaa
    17701 aggttgcacg ccttcgacca tcttttgttg cgtatttttt gataaggcat tttttactag
    17761 ttctttaaaa gaaagcttgc ctgctcttga aacagcaaat tgcttcgttt ctgtgtattc
    17821 gatgtcacct atccgccata tgcggataac tgtaccattc gcctgcatgg caccatctac
    17881 ttcagcatct gtagcccaat aagggaaata cacacctgtc aacttatcga tttgatcctt
    17941 attgaaaaaa gctttgggaa caaaccattt tttcttggtc caagcgagaa atctgttgat
    18001 tgcttcttct ttctcgacag caaaaggaag tactttattt ggcaagaatt ctccgcttaa
    18061 acgtcccgat agtacgacgg gattatggca gtagtaacaa taagtcgcag cagttgtcgc
    18121 atcagtcacg atctctgcac cacagttggg acaaagaaaa agatctatcg ctgcggtatt
    18181 atctgcagat tcgtccgctg tatctgaaaa accgcctgcc tcgtcaaccg ctttgcgctc
    18241 ggcttcattc atatcggcaa gctgttcttg ggctgtaaaa gtaaaatctt cggtaggtga
    18301 aggttcttct ttgctttctt cgccagccat acgcgcttct ttttgctttt gctcatattg
    18361 gctgacttct tcttctgtat agatattcag gcagtattcg caatgaaatt tttggtcttt
    18421 gggatcaaaa gtcaacggac cgccgcaatt aggacattta tgtgtaagaa catccatcag
    18481 ctgcacacct ttctaaataa attaattaac ggggattcct ttaaacggtt taccacagtt
    18541 tggacagaat ttagggatgc cgttggaaag atccacgatc tcattgcatt cactgcatcg
    18601 catttgtagc ttaggctgaa cgttttctac aggtttggca gctccgcagt tggaacagaa
    18661 ttttccggaa ttttctgtcc cgcaaactgg acatgtccaa gtatccgcat ttccttgtgc
    18721 agtttgttga ttttgttttt ctgcttgttg tttgatttgt tcttggttat tttgagctgc
    18781 ttgagaaaga taagaaccat ttgcattcat tcccatacct acacccataa agccggtcat
    18841 tgctcctgct tcattttttc cggctgcttc cataccacgt gcaatggaac cttgcacgta
    18901 tccttcacgt acgctaggat cacctagcat cgccccttcg ttacgcatat tgatcagttt
    18961 cacagaatca tcggtgtaag aaatgctggc tacagccaca gaaacaatct ccatcccacg
    19021 taattcacgc caagaatcat ccaatgcagt atccatgtat ttgcttaatt ccaaactctt
    19081 agatggtaca taagaaatcc gttgaccatc tgcagacatc tgattgattg ctgattgtag
    19141 cgctgttagg aattcagcta aatactgctc attgatatca ttgatttcca cttgtgtttt
    19201 gtttttaggg attgcgtttg tataaaataa aatcgggtca acgacatgaa tagaataagt
    19261 gccatgtgcc cgtaagaata attctgcgtt atagaaattg tcaaagtagt tcaacggcgc
    19321 gcttgtaccg aatttgattc cctttatttc ttggagattg atataaaaaa cttgttgttt
    19381 ctgcggtgtg acgccgccaa atttgaatcg gtcaaaagtt tcagcaattg cttctttcag
    19441 tgttccattg aacatggaag gagctgcgtc atttttaatg gtgtaataac cttcctcagc
    19501 ggtataatcg atgatttttc caccatcgac caaaagcatc atcatgtttg gatagacatg
    19561 gacgactgtt ccgtctgtta aaacatcttc tgttccttta cgattggagc cacgtttgtc
    19621 atctttgcgg actttgacac cttttgtcat gaccgttgta tcactcatat tgtctggttc
    19681 gatgacttct agccattgat ctgccagacc gccgccaacc atacttgttg ctgctttgat
    19741 aagacccata aaaaattcct cctctgattc agctagtgcc aaacaattat tgtttattta
    19801 ttataccata ggaagatgtg aaacttctcg gactagagac ggaattgaaa tggaaaattg
    19861 cgtattgttt aaatacctga aaaagccgag aaggatagta gtttttttac ctaatatcac
    19921 gtataataag agatagtgaa ttttttaaaa aagggggatc cttatggcat atcaagcact
    19981 ttatcgagtc tggcgttcgc agcgtttcga agacatcgtt ggacaaaaag ctgtgacaca
    20041 gacactaaaa aatgctattg tttcccacaa aacctcacat gcctacttgt ttacaggacc
    20101 cagaggaacc ggaaagacga gtgcagcaaa gattttcgca aaagcaatca actgtccgaa
    20161 cagtaaagac ggtgagccat gcaacgaatg cgaaatgtgt cgatcaatta ctgctggtac
    20221 gcaagaagat gtgatcgaaa tcgatgctgc cagcaataac ggagtcgaag aaatccgatt
    20281 tatccgtgat cgggcaaatt atgcgcctac aaaggcagcg tacaaagtgt acattattga
    20341 tgaggtacat atgctttcaa caggagcttt caatgccttg ttgaaaacat tggaagagcc
    20401 aaaagaaagt gtcatattca tcttagcaac gaccgaacct cacaaaatac cagctacgat
    20461 catttcacgg actcagcgat ttgattttaa acggatcaat acggcagata ttgtcgaaca
    20521 tcttgaattt atcttgaaag aatcgactat cagctatgaa ccagctgcat tgaatgtgat
    20581 tgcccgttct gcagaaggcg ggatgcggga tgcattgagt attcttgatc aggcaatctc
    20641 gtttagtgaa ggaacaatta ctttagatga tgccatgcaa gtgacaggta gcttaactga
    20701 cgaaatgatg gacagttact tgagtgcttg cgtgaaaaaa gaagtaccag aagcattgga
    20761 aacattgaac caaattctcg cagccggcaa agaatcacgt cgctttctag aggatatgtt
    20821 acaatactgc cgtgacttgc tgatgtatca gcaagcacct aacctagttg aggaacaatc
    20881 gagtacgatc agaagtacat ttacagaatt ggcacaaact gtttcagcgg atcagctgtt
    20941 ttcaatgata aagattttga gcgatactca aaatgaaatt cgttttacca atagtgccgc
    21001 tatttatttg gaagtcgcta cagtcaaact tgcacagcta ccgtctgctt ctgttccttc
    21061 agcagatgta gcatttgcag atgatcagat agtaaaacag ctacaacaag agattaagga
    21121 actaagaaac gaaatcaagg aactacgcag taatacagga attgagaata caaaagtaca
    21181 gccggtacag caaacaaatc ataaaaaaca gacaagtacc tatcgtgtac cgacggaacg
    21241 agtatataaa gttctgcggg aagcgacaag acagcatctg aaccaagtaa aagaagtatg
    21301 ggaagacttg ctgatgagtc tatctgtcac tcagcgagca atgcttaaag caagcgaacc
    21361 agttgcagct ggacctacag gactagtgat tgcgtttgat tatgagattg tttgtcaaag
    21421 agcagcaaat gacgaagaat tgcagctttc ggtacacaac aacttaagcc gaatgatcaa
    21481 agattatgcg cctgatcctg tttatattac aagagaaagc tggccagaac ttcgaagaaa
    21541 ttatttgacg caggcacaag aagagggaac agaaaactct tttgcttctg aagactcaga
    21601 aggagaaatc gagttgcttc ctcaagaaca agaagtacca gaagaaacac ttgtagtaaa
    21661 agcagaggaa ctgtttggct ccgaagctgt tacagtggta gatgattaat aaaaaggagt
    21721 agataattat gatgcgtgga atgggcaata tgcaaggcat gatgaaacaa gtacaaaaaa
    21781 tgcaaaaaga aatggcagaa gcacaagagc tgctaaataa aacagagttt acaggtaaag
    21841 ccaccaatga attggtcaaa gcggttttta caggcgatcg tcgcatgaaa gatatccaaa
    21901 tcaatgaaga agtcgttgat cctgatgata tcgagatgct tcaggatctt gtcgtaatgg
    21961 cagtcaatga tgcgatcgca caaattgatg tggaaactga gaaaaaatta gggaaatacg
    22021 caaaaggttt gccaggattt tagttttaaa ctgaaggagt aatgaatatg caatatccag
    22081 aaccaattgc gaaactgatc gacagctaca tgaaacttcc aggaatcggt cagaaaacag
    22141 cgacaagact cgccttttat acgatcgata tgaaagatga agtagtaaat gaatttgcta
    22201 aatctttgtt aagtgtaaaa cgcgatttgc atttttgtag tatctgcgga aacatcacag
    22261 aagaagatcc ctgcgaaata tgtagaaatc cttcacgtga tcgaagcgtt atcctagtgg
    22321 tcgaagagcc gaaagatgta atggcaatgg aaaaaatgcg agaatatcac ggactttatc
    22381 atgttctcca tggtgtgtta tcaccgatgg aagggacagg tccagaagac atcaatattt
    22441 cttctctgtt gcagcggctg catgatgcag atgtaaaaga agtgattatc gcaacaaatg
    22501 caacaacaga gggagaagca acagctatgt atctctcacg tctgatcaaa cccgcaggta
    22561 tcaaagtgac tcgtctagca catggtctgt ctgtaggctc tgatatcgag tatgcagatg
    22621 aagttacttt attgaaagct gtcgaaggaa gacgtgagtt ataaatttct aattaagtgt
    22681 tttttcagat acaatataaa agcagcacaa gagatggggg caacaatgtg aacggactat
    22741 ttattacaat cgaggggcct gatggtgcag gaaaaaccag catcttgaat gaactttacc
    22801 ctcttttgaa agaagtagca aaaacagaaa tcattcaaac aagagaacca ggaggcattc
    22861 cgattgctga agagatccga gcagtgatcc ttgatccaaa aaatgatcgg atggatgaac
    22921 gtacggaagc actgctttat gcagcagcaa gaagacaaca tctagtcgaa aaggttctgc
    22981 ctgctctggc aaaagggaaa atcgttttat gcgatagatt tgtcgacagt tctcttgctt
    23041 atcaaggtgc aggccgacgc atcggcgtta ccgagattgc tcggttgaat gagtttgcta
    23101 cagaaggtac tacgccggat tttacccttt atcttgacgt agattcagac accggattaa
    23161 gaagaatcaa gaaaaacaga caaaatcaaa tcgaccgttt agattcggaa ggtttggaat
    23221 tccaccagcg tgtacgtcat gcttacttaa agctggcaga agagaatccg gaaagaatcc
    23281 acaaagttga tgcaagaaaa agctttgaag aagtgctgca aacaagttat catacaatta
    23341 ttgaacagta tccgcaattt tttgaaaact aggaaggtga acgtttatga aaattatttt
    23401 agcgattatc caagacaaag acagcaaccg tttatcaaat gaattgattg atgcaaatat
    23461 ccgtgccaca aaactatcat cgacaggcgg cttccttaag gctggaaaca gtacgtttat
    23521 cgtgggtatt gaagatgacc gtgtagaaga agcattggaa atcatcaaaa aaacgtgtga
    23581 atcaagaaag caatttgtat ctacacctgt tacactggat atttcgatgg atgggggcgt
    23641 cccttatcca gtagaagtag aagtaggcgg tgccacagtc ttcgtcttac ctgttgaagg
    23701 attccatcaa ttttaggagg agctgagcta tgaatcaaga aataaacttg gctcaaatgc
    23761 aaccgatcgt gtatcagcag cttcaacgga gttttgagca tgggcgtatc gctcatgctt
    23821 atctttttga aggggagaaa ggaacaggaa aacatgaagt aggtatatgg cttgcacagc
    23881 acctgttctg tacccaaatg aaagacaatc tgccttgtgg aaaatgcaac aattgtcagc
    23941 ggatcgaaaa gaaagaacat ccagatgttt tggttattga accggaagga caaaccatca
    24001 aagtcgatca gatcagacgt ttgcagacgg aattcagtcg cagcggttac gaatcaagaa
    24061 aaaaagtttt tttgatcaaa gaagctgaaa aaatgaatag cagtgcggca aacagtttgt
    24121 tgaaattctt ggaagagccc cctggagatt ttttagcggt gttagaaaca gatgcagtag
    24181 gaagaattct ccctactatt caatctcgtt gtcaagttct gcattttcaa gaactttcga
    24241 aagaagcact gatccatcga ttgcaagaag agcagattcc attggaaaaa gcaaaacttt
    24301 tggctttctt aacaaacagt ttaggaaaag cagttgaaat ctctcaagat gaatggttta
    24361 atgatgctaa ggacttgatt tatcaatggt tcgtttactt gcaaaagcaa gatactcaag
    24421 cctttatcta tgtccaaaaa aaattggtaa aatcatttaa agaaaaaaca caacagttta
    24481 caggattatc tgtcttgatg ttttattatc aggaagcaca gcgtcaagca gtagcaaacg
    24541 gcgaatatgg caaagtgaaa cagataaacc agataattga acggatattg atcgcagagc
    24601 agaaactacg cagcaatgtc agttttcagg ctgttgcgga acagttcgtt ttgcatacga
    24661 ttttttagct tgccgcaaag gatgatgatc aaggaggatg gaaatggtag aagtagtagg
    24721 tgtccgatat agagatgcag gtcatatcta ttattttgca ccaggagact ctgaatatgt
    24781 ttatagtgac aaggtattag tcgaatctca gcaatcctta catattgcta ctgttgtcct
    24841 tcctaaaaaa gagattgatc ccaatgacct tcctgaagct atccgtccga tcttgcataa
    24901 agcgtcacaa gctgaattag aaaaagacaa aaaaaacaaa aaagatgctt atgatgcatt
    24961 gaaaatcgca aaagaaaaag tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga
    25021 atacacattt gatcgaaata aattgatttt ttctttcaca gcggacgggc gaattgactt
    25081 tagagaattg gtaaaagatc tggccgctgt atttcgtact cgtattgagc ttcgacaaat
    25141 tggtgtccgc gatgaagcaa agataatagg cggaattggt ccatgtgggc gtccattatg
    25201 ctgttcttct tttttaggcg atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag
    25261 tttatctttg aatccaacaa aaatttccgg tttatgcgga cgcttgatgt gctgtctgca
    25321 atatgagagc gaagaatacg agacagcgaa aaaagaactc cctgactttg gaaaagtaat
    25381 tgctacacct gatggtaaag ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag
    25441 tattcggctg actggacggg aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt
    25501 tatccaagta ggtgagatga atggataaga gatcattata cgacggattg aatcaattag
    25561 aatcagagtt gagaaataca ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga
    25621 tcgtagaaaa aaatacaact ttggagatgg aaaatcaacg tctgcgggaa cacctgcggg
    25681 aattgaacca agttgcacaa gctccagctg aaaatgtgaa gcaggaactt tctaagtctc
    25741 gtatgaatct tgaaaaaatt tatgaagaag gattccatgt ctgctacgat ttatatggat
    25801 caagaaggga caatgacgag ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg
    25861 gtcgatgacc aataacaaga ggagactgga gcggagaaat actgctccag tctttttatt
    25921 aaggagattg tgacagatgt ataatcaaaa aagtttcaag ggaaatccgg tatatgggga
    25981 tttatatctt gtaccgacgc caattggaaa tttagaagat atgagctaca gaagtgttcg
    26041 tatgctgcaa gaatccgatc ttatcgcaag tgaggatacg cgtaatacac aaaagttatt
    26101 gaatcatttt gaaatccata caccccaaaa aagtttacat gaacataatt ataaagaacg
    26161 tgtccctcaa ttgattgctt tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc
    26221 aggaatgcct tccatcagcg atcctggaca tgagctagtt ttagcctgca taaaagaagg
    26281 gatttctgtg atctctatcc caggacctac agctgggatg acggctttga ttgcttcagg
    26341 gctagtgcct caaccatttt tattttatgg cttccttcca cgtaagaaaa aagaacagaa
    26401 agaaacattg aaaaagctaa aagaacagac agttacgctg attttttatg agtcacctta
    26461 tcggatcact gctacattga cgaatttctt agaaattttt ggaaatcgcc aagtcgtttt
    26521 gtgtcgggaa ttgacaaagg tccatgaaga ataccttaga ggaagtacgg aagagctact
    26581 cgcttatatc gaagaacatc cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt
    26641 gttcgaaatg ccagtgacgg aagttgatga agagttggga acgttaaagg aacaagtaga
    26701 agcaaagatc gcttcaggcg aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa
    26761 tgggctgaaa aaacaagaag tctaccgtca gtaccatgaa ttagactgag atagtataag
    26821 ttgtctataa tgaaagacta atgaggaaat cagctactaa cttttcaaat ctcttgaacg
    26881 cgaacgaatg ttcttgtata atgaataaga ggtgaggatg atggagctgc aatttccttt
    26941 acataatgat acatcaagaa aaatcattca tattgatatg gatgcttttt tcgcttcggt
    27001 tgaagaacga gatcatcctg agtttcgcgg acatccatta gtaattgcca gacaccccag
    27061 tgacactggt gggcgtggag tagtgactac agcaaattat gaagcaagaa aattcggaat
    27121 tcattcagca atgagtgcac aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc
    27181 tggcgaccat cataaataca gcgatatttc caaacaagta cgggaagtat tctatcgtta
    27241 tacagatatt gtggaaccag ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa
    27301 aatcaattgc aagtcagcca tcaaaatcgc ccgaatgatc caagcagata tttgggagag
    27361 tgtccagcta acatgttctg cgggagtaag ttacaataag tttttggcca aattagcatc
    27421 agactatcaa aaacctagag gaatcacggt agttgcccct caagacgctg tgccattttt
    27481 aaaagctttg ccaattgaaa aatttcatgg ggtagggaag aaaactgtac ctagaatgca
    27541 tgagctaggg atttatacag gaaaagacct atatgaatgc acagaaatga tgttgattcg
    27601 aaattttgga aaaatgggct attctcttta tcgtaaagtc agaggaatcc atgattctcc
    27661 agtaaacgtt acaagagagc gaaaatctgt gggaaaagag catacttatg ggcagccgtt
    27721 gcaaacggaa gaagcagtgc tgactcagtt gcgccaacta gctgaaaaag tagaagaagc
    27781 actgagaaga gtccaaaaac atgggaaaac agtagttttg aaggtcaggt acacggacta
    27841 ttctactgtg accaaaagag tgacattgcc agaatatatc tataaaaaag aagcactctt
    27901 ttatcaagca agccttatat gggaggaaat cttgggagta gaaaaaggaa tccgattgtt
    27961 agggattact ttgacgaatc tggacccgat gacttatgaa aacatcgtcc ttccattatg
    28021 ggaaaatcaa gaaatataaa aacagctttg agagttgaaa ccggaaatcc gttttctctc
    28081 ccaaagctgt ttttatatca agtcttgctt tttgatttgt tcataggttt ctgttgcatc
    28141 cgtacatttt tcccaaatca caacttgatt ggattctaaa gatttttgga cgtcaatgat
    28201 ccgctggttg ctacttccgc gaaattgtag ttttaggtca cgtttgctta attcaaagcg
    28261 tccgtcaaca aggatatcga tttgcgaaag gagttctaat ttatcttctg aatcttgtag
    28321 caattcatca aaggtatagc ctgtccatga ccagatatct tttgtcgtac cgaactcttt
    28381 tcggatacgt cgtacgactt gcagacaaac atctgtattc aaaaaaggtt cgccaccaag
    28441 taaagttaat ccttgcacgt aatcgttgcg caagtcagcg atgatttgtt cttctaattc
    28501 aggtgtgaat ggtttgccat atcggaaatt ttgtgcagct ttgttgaagc agccttcgca
    28561 ggcaaataag cagccactaa cgtaaagact gttgcggaca ccttcaccat cgacaaaatt
    28621 aaacggtttg tagtcagcaa tatagttttg gctgtagtct tttgctaacc attcttttgg
    28681 ttttggattt ctcataaaaa aacttctttc atttaaggtt caaagatata gctattgaag
    28741 ataaaatcta ctcgaccaca taaataagaa caaactgacc taaatccatt gttcaagaaa
    28801 gcttgttctt attttgatag agggcgttca aagatacaaa gccatcgaaa gagggtggtg
    28861 aaaaaagact tgtcatagcc tcttattctg aataaacggt gttcaaaagc ttgcatctgc
    28921 agtaataagc ccaactaaac gaaaaatatg aggaactatt tttgtttagc tattcttatt
    28981 acttgatgca ggacagcttt ttcacaacct catttcatgt gtttcacacg tgaggaaatt
    29041 tctttatggc ggccatgtac catcggacga gcttgcggat ttcctaaata gccacaagta
    29101 cgtttgacta catcacatgt ttttggatca tgatttccac attctggaca ttcgaaccct
    29161 cgttctgtcg gtttgaaatc accttcgaaa ccacattgat agcaatgatc gattggcgta
    29221 ttcgtaccaa gatacccgat tttgtcatat gcaaaatccc atacagcttc taaagctttc
    29281 ggattttgtt ggagcatagg gtattcacag tagtggatga atccacctga acaatatttt
    29341 ggataatcct tctcaaattc caatttttca aatggcgttg ggtttttacg cacatcatag
    29401 tggaaactgt ttgtataata ttctttatct gtgatatttt cgatgatgcc gaatttttct
    29461 gtatccaaac ggcagaaacg gtcagttaga ctttcacttg gagttgaata gacactgaaa
    29521 tgataaccat attcatcccc ccatgcatct gcattagctt ttaattcttt gacgatatca
    29581 agtgtgaaag cttttgcttc tgggtttgtt tcccattcgc caccgaagaa agctgaagca
    29641 acttcataca atccaatgta gcctaacgat acagtagctc gacggttttt gaataattca
    29701 ttgacagaat cggtacgtga aagacgtttc ccgaaagcac catacatata taaaattggc
    29761 gcatttgctg gaatggcttc tttacaacgt tctacacgat agagaagcgc atctttcatg
    29821 atttctaagc ggtcagacag taactgccag aatttattct gatctccttt tgattcgatc
    29881 gcgattcttg gcaaattcaa tgtaacaaca cctaaattca ttcgtccgac atttacttct
    29941 tgaccatttt cgtctttcca cccttgcaag aatgaacgac agcccatagg tactttgaaa
    30001 cttccagtta attcaacgat tttatcgtaa ttcagaatat caggatacat tcttttcgtt
    30061 gcacattcca atgctagttg tttgatgtcg taattaggat cttcagggtt taagttcaca
    30121 ccttttttca aagtgaagat cagtttaggg aagatagctg tccgcttttc actccctaat
    30181 ccattgattc gaatctgtaa aattgctttt tgaatttctc tttcaaacca gttttctcct
    30241 agtccaaatc ctaatgaagt gaaaggagtt tgtccatttg aagtaaataa ggtattgatt
    30301 tcatattcca aactttgcat agcatcgaag atatcttttt tggtttttgc ttttgcatat
    30361 tctttttggc gttctggcga atcgatccat tcttccgcgt cttttagatg ttttttatag
    30421 ttcaattctg caaaaggagc aagcaattca tctgttcggt cggcagaaca gccaccatat
    30481 tgacttgacg ctacattggc aataatctgt gagatttgtg ctgtggcggt ctggatagat
    30541 tttggcgatt cgacttcggc attgccgatt ttgaacccat tgttcagcat gcctttgaaa
    30601 tcaatcaagc agcaattcgt catcggtgta taaggatgat aatccaaatc atggtaatgg
    30661 atatcgcctt tttgatgcgc atttgctata tgtggtggca acattttcag tccgattgat
    30721 tttccaacga tcccggctgt cagatctcgc tgtgtattga aaacattact gtctttattt
    30781 gcgttttcgt tcacgacagt ttggtccttg ttgattagct tttcaatcga aaagttgata
    30841 tctgttgctt tactgcgagc aaaatctcgt ttggtacgat aattgatgta ctcttctgct
    30901 aattcatatt cgtttttttc tagtaatata tgttccacga tgttttgaat ctcatagatt
    30961 ttgacatttt cagcaaaccg gcttttgatt tcttggacaa ccaagtctgc aatcatttga
    31021 attttttcat aagtaagtgg tgataatgag ccgtgaattt tcttttctgc tttgatcagt
    31081 gcttcattga tcttttgttc atcgaaatca acatggcggc catcgcgttt gatgactttc
    31141 atctgggatg tctctggctg ctgagtaacg acagtttttt cttcataatt tactttcatt
    31201 atcttcaact cctgctaaat tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa
    31261 ctaaatatcg gctatatata gtattcaatg tgctg
//
LOCUS       DAZKCY010000023        30932 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000023 DAZKCY010000000
VERSION     DAZKCY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.023"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..311)
                     /locus_tag="ACG8XC_000953"
     CDS             complement(147..311)
                     /locus_tag="ACG8XC_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190668.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            606..1934
                     /locus_tag="ACG8XC_000954"
     CDS             606..1934
                     /locus_tag="ACG8XC_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFP9190669.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2136..2969
                     /locus_tag="ACG8XC_000955"
     CDS             2136..2969
                     /locus_tag="ACG8XC_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP9190670.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2985..3722
                     /locus_tag="ACG8XC_000956"
     CDS             2985..3722
                     /locus_tag="ACG8XC_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFP9190671.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3877..4845)
                     /locus_tag="ACG8XC_000957"
     CDS             complement(3877..4845)
                     /locus_tag="ACG8XC_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP9190672.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5077..5910
                     /locus_tag="ACG8XC_000958"
     CDS             5077..5910
                     /locus_tag="ACG8XC_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP9190673.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5944..6783
                     /locus_tag="ACG8XC_000959"
     CDS             5944..6783
                     /locus_tag="ACG8XC_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9190674.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6809..7369
                     /locus_tag="ACG8XC_000960"
     CDS             6809..7369
                     /locus_tag="ACG8XC_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9190675.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7568..9289
                     /locus_tag="ACG8XC_000961"
     CDS             7568..9289
                     /locus_tag="ACG8XC_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFP9190676.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9454..10596
                     /locus_tag="ACG8XC_000962"
     CDS             9454..10596
                     /locus_tag="ACG8XC_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP9190677.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10612..11823
                     /gene="thiI"
                     /locus_tag="ACG8XC_000963"
     CDS             10612..11823
                     /gene="thiI"
                     /locus_tag="ACG8XC_000963"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP9190678.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12396..13043
                     /locus_tag="ACG8XC_000964"
     CDS             12396..13043
                     /locus_tag="ACG8XC_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP9190679.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13172..13388
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13436..16081
                     /locus_tag="ACG8XC_000965"
     CDS             13436..16081
                     /locus_tag="ACG8XC_000965"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP9190680.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16391..17704
                     /locus_tag="ACG8XC_000966"
     CDS             16391..17704
                     /locus_tag="ACG8XC_000966"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFP9190681.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17694..18347
                     /locus_tag="ACG8XC_000967"
     CDS             17694..18347
                     /locus_tag="ACG8XC_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9190682.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18402..19094
                     /gene="radC"
                     /locus_tag="ACG8XC_000968"
     CDS             18402..19094
                     /gene="radC"
                     /locus_tag="ACG8XC_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9190683.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19244..19444
                     /locus_tag="ACG8XC_000969"
     CDS             19244..19444
                     /locus_tag="ACG8XC_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9190684.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19698..21377)
                     /locus_tag="ACG8XC_000970"
     CDS             complement(19698..21377)
                     /locus_tag="ACG8XC_000970"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP9190685.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21379..21591)
                     /locus_tag="ACG8XC_000971"
     CDS             complement(21379..21591)
                     /locus_tag="ACG8XC_000971"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFP9190686.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21984..23714
                     /gene="efrA"
                     /locus_tag="ACG8XC_000972"
     CDS             21984..23714
                     /gene="efrA"
                     /locus_tag="ACG8XC_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFP9190687.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23711..25471
                     /gene="efrB"
                     /locus_tag="ACG8XC_000973"
     CDS             23711..25471
                     /gene="efrB"
                     /locus_tag="ACG8XC_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFP9190688.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25566..25763
                     /locus_tag="ACG8XC_000974"
     CDS             25566..25763
                     /locus_tag="ACG8XC_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190689.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25916..26401
                     /locus_tag="ACG8XC_000975"
     CDS             25916..26401
                     /locus_tag="ACG8XC_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP9190690.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26505..27098)
                     /gene="clpP"
                     /locus_tag="ACG8XC_000976"
     CDS             complement(26505..27098)
                     /gene="clpP"
                     /locus_tag="ACG8XC_000976"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP9190691.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27278..27805
                     /locus_tag="ACG8XC_000977"
     CDS             27278..27805
                     /locus_tag="ACG8XC_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190692.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27896..28633
                     /locus_tag="ACG8XC_000978"
     CDS             27896..28633
                     /locus_tag="ACG8XC_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9190693.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28637..29554
                     /gene="miaA"
                     /locus_tag="ACG8XC_000979"
     CDS             28637..29554
                     /gene="miaA"
                     /locus_tag="ACG8XC_000979"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP9190694.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29556..30785
                     /gene="hflX"
                     /locus_tag="ACG8XC_000980"
     CDS             29556..30785
                     /gene="hflX"
                     /locus_tag="ACG8XC_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFP9190695.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(30819..>30932)
                     /locus_tag="ACG8XC_000981"
     CDS             complement(30819..>30932)
                     /locus_tag="ACG8XC_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9190696.1"
                     /translation="FISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT        10216 a         5286 c         6597 g         8833 t
ORIGIN      
        1 ctgccggagt aaataaagct tatgcaaaga aaaagaaatc caatcacttg ggtttctttt
       61 tcttttatta ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac
      121 gaaagagtag agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa
      181 taaatcagca ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc
      241 ccaaagttat ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc
      301 aggtttttca ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt
      361 attttgaaag ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc
      421 attttcgtta tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac
      481 atttttcttc atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga
      541 gattgtattt ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg
      601 attggatgaa agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa
      661 atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg
      721 aaaatgtttc agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga
      781 aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc
      841 gtcataagat gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt
      901 ttttctggga taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa
      961 aagaggcaat cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg
     1021 gccaatggga tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga
     1081 tgccggaaag tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac
     1141 tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta
     1201 ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag
     1261 gaacacctcc aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg
     1321 accgaggact gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt
     1381 atgttagcgt gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag
     1441 agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa
     1501 ctttcaaaaa attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg
     1561 atgtaggtca atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg
     1621 atgatctatt tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa
     1681 gtctgatgac ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa
     1741 aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga
     1801 gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga
     1861 aagagcaatt aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg
     1921 gtgctttagc atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg
     1981 cttaagccag ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta
     2041 taattctaaa taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac
     2101 gaagtagaat aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg
     2161 atctgaaatt aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc
     2221 gtataaaaac cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat
     2281 tttttgaagc agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata
     2341 cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg
     2401 gaaaagggga tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc
     2461 cacaatttgg ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg
     2521 aagaagattg gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag
     2581 aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg
     2641 tattgaaatc ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga
     2701 gtggtaatgt gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt
     2761 atgggaatcg ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat
     2821 ttttagatgc ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga
     2881 tttaccagtt atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc
     2941 aagtagatca attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata
     3001 ccaaaaagca tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt
     3061 cgatgttagg agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca
     3121 gtgaattcgt caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta
     3181 atcgtctccc tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata
     3241 agtttttctg ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa
     3301 tagaatctgt tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag
     3361 agatcattgc tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg
     3421 aaaagattga ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta
     3481 atcagcatgt taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact
     3541 ttttgactac gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg
     3601 ggaatgagat cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac
     3661 atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg aaaaataaat
     3721 aaaaaagagg ttgtgacaga agggggcaac ttcaagaaat aagaagaaat ttacgaaaat
     3781 tgtttctcaa atttttgtaa attttcgact tagttttcga agaagctatt tttgtcacag
     3841 cctctctttt taagtttagc tattagataa tgatgtttag ttttggataa acgaaatcaa
     3901 ttcttcaccc gacaaaccat agtttacagc aatcaatagt tttaaacgtg ctttttgact
     3961 attaatactg ttacagaaaa taatccccat tttttctagt tcttttcccc cgccttcata
     4021 atcataaaca ggctctgcga ttccattgaa acaacgagaa acaagtacta cagggatttt
     4081 ttggacaaga agcttttcca aagcagctaa ggcttgtgga gggagattcc ccgcacctaa
     4141 agcttcgatt accaaaccgt ctactttcgt atgggcgatg gcttccaata agtccccttg
     4201 catcccagca tatgctttaa cgatcgggat agtcccatct acagcggaaa tgtccagtcg
     4261 ctttgtttct aaaagttcct gcaagaataa aattcgattc ttagttacta agccgatggg
     4321 acccaatgta ggtgttcgga aagtagcaac atttgtggta tgggtctttg tcacgtagcg
     4381 tgcagaatgg atctcatcgt tcatgaccac caaaaccccc ttatctagcg cttcttcaca
     4441 acttgcgaca cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa gttcattact
     4501 tgaacgcatt gccccagtca aaacaattgg taatttgttg ccaatcgttg tatcaagaaa
     4561 aaaagcagtt tcttctaatg tatctgtacc gtgtgtgatc accactccgg atatacctga
     4621 tgcataagcg gacaagatac gttcttttaa taccaacatg tgagaaggca tgatgtgtgg
     4681 tgagggaaga ttgaagatat cttcaactaa taattctaca tgcttgggga ttgtgatttc
     4741 tgcatctaat aatgggtttg ccacatcagg actaacaccg cccgtgagca tgttttcttt
     4801 catggcgatc gttccgccgg tgtgtagtac aagtattttt ttcattttac agacttcctt
     4861 acgaataatt ttgcatagcc gttaaatctt attgtatact atataaaaga gttttgctat
     4921 cagtgaagaa taacgaatag cctaacttat cataaaaaag ctgaccttat taaaatggaa
     4981 aagactcaac cttgacctcc taatacaatg gagtatctgg ttgagtttct tttacaagaa
     5041 agcagccaag gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg attaacatcg
     5101 tttaaagaac atgacaaatt aactggtaaa aagaataaca ctctttatga atatgcaggg
     5161 tttctaccct tagtggaatt agatactgcc tattatggaa ttccccgaaa gtcctcagtt
     5221 gagaattgga taagtgaagt acctgaatca tttcgctttg tagtcaaagt ctatcaagga
     5281 atcagcggtc aaggagactg gaagccttat tatcagaccg aagaagagat gattgcgcat
     5341 tttttagatg ttatgtcgcc aatggtagat gctggaaaga tgttttgctt tttagtccaa
     5401 ttcccctcat cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa acttagaaag
     5461 tggtttgagg gttatcctgt ttctatagaa ttgcgggatt attcttggta cagcgatgaa
     5521 tttcttgaac gtacaagaaa ctttatgtct agagccgctt tttctttagc catcatcgat
     5581 gagccacagt tgctgaatac aacgattcct tttgatccat ttgtcaccaa tccgaacttt
     5641 gctctttttc gattccatgg taggaatgcg caaggatggc aagcaaatga taaagagtgg
     5701 cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac tgcaagaact agcacatgaa
     5761 gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga tttttaataa taattctggt
     5821 ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat taaatatcaa gccagatgga
     5881 ctcaatccaa cccaaatgga tctgttttaa gggcttattt tattcaagaa aggggcgaag
     5941 tctatgatca aagctatttt ttttgatatt gacggaacat tggtcaccaa tcgttcaaaa
     6001 gcgttggaat caacaaaaca agcaattgaa tatgctcgga aaaacggcat tttatgcggt
     6061 gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt ggaattagat
     6121 atgtatgtcg tgtataatgg tcaactggtt tttactgcag atcgcacgat tatcgaccac
     6181 ccttttgaac aaaaagtatt ggaacatatt gttgaatttg cagatgagaa ccatcgacaa
     6241 atcgtttttg gtgcgagaaa tcggttagat ggatcaacga cgatgctatt aggacaatcg
     6301 atttttatca agcgactagt cagcttttta ccgcgtaaat tcccggtacg tctgatgaaa
     6361 aaaatcctcc aggtttttag ccctcaccga caaaaggatc gttatgaaac gttagagata
     6421 ttaaaagaac caatttatca atgtatattg ctcagtccgg agtcagaaca gaaaaagtta
     6481 gaacatcgct ttcccgagtg tacgtttcag cgttcaaata cgtatactgt agatatcatc
     6541 ccaaaaggtg gttcaaaatt actaggcatc caagctttcg caaatgctgt cgaaattgaa
     6601 atggaagaaa ttatggcatt cggcgatcat tacaatgata tcgagatgct gaaaggtgta
     6661 ggaattggag tggccatggg gaatgctcag atcgaagtaa aacatgctgc agactttgtg
     6721 actcaatcaa atgaagaaga tgggatatac gaaggactga aacacttcgg tctgatttat
     6781 tagtcaatga aagaaggaaa aaatcagtat ggaagcagag acaccttttc aaaaagtaga
     6841 aacattccac aaaacttttg atccaagaag accagagata cccacggctt ttacagaaaa
     6901 tgaagctcat acacgtgctg cgttcaaaat agaagaactt gttgaatttg ttgcagctac
     6961 ttcacctaat aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag cattagataa
     7021 agcagaagaa aaaatacttg gtaaaacaca gtggggaagt gatccgctgg ttgaacaagt
     7081 agatgcactg acagatatcc tttactttac ttatggttct ttttcattac taggaatagt
     7141 gcctgatcga atattcgaaa tcgtccatca ggcaaatatg gggaaattat ttccagatgg
     7201 aaaacctcat tatgaccctg taacaaataa agtgttgaaa cctgaaaact ggcagatgga
     7261 atatgctcca gaacctaaaa tcgcggcaga aatccaacgt cagattcaag aagcgattgc
     7321 taagaagaaa tccgctgata taggcatgtg cacaaatgaa aaagagtgat cagagtttca
     7381 ctatttttgt tatctttata cgatggaaaa acaaaagatt ttcgtttaag ttagtgaatg
     7441 catgaaaata gtatgctata atctagaata gatgtctttt gcacgagaga cgtattattt
     7501 ggatttatgc caagcagaac gccatctgtt gcataaaaga tgcatactga aaacggagga
     7561 accataaatg ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta tagtcgccat
     7621 tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac caagatcgat tggatgcctt
     7681 agaaaaacgc aaggaagaat tatttgactt gccagtgatt gaagaagtcg atgatgttaa
     7741 aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt gaatggagtc aaaaatggaa
     7801 tgacatatct acttcctcgt tcgctgaact agaaagtcaa attttcgaag tggaaaacct
     7861 caatgaaagt tttcggttct taaaaggaaa aaaagcagtt gttgaagcag aagctacaat
     7921 ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat tgcgggaaac
     7981 ggaagaacgc aactcactag ctgttcagca ggcacttgac gtctatgaag aactgaaaga
     8041 aaaagtcaga gaagacgagc atgaatacgg accagctctt tttgaattgc aaaaacagat
     8101 caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta aacacttctg gagaccctgt
     8161 tgaagcccgc gaggttttgg ataaagcaga aaaacatacg tatgaagtgg aagctctgat
     8221 gaaaaaaatt ccaccgctgt atgaggagct ccataccact ttccctgaac aacttgaaga
     8281 aatcagtgat acttacgaga aattgatgga agaacaatat gtattcccag aagaagatct
     8341 tgctgaagat atcgcaaaag taagtcgaag aattgaaaat tcattagcca atcttgaaaa
     8401 aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga ttgattcgtt
     8461 atatgatatc ttagaacggg aaatggaagc tcagagatat gtaaaaacta accaatcaac
     8521 gattgcagag tatatcaaac atacaacaaa gaataaccgt caattattga ttgagctgga
     8581 tcacacatct caaagctata cattgaacca caatgaaatt ggccgtgtac gaggtttcca
     8641 aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg attccacaga tccgtcaaca
     8701 cgaaattcct tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag tcttagaaga
     8761 tatcgaaaag caacaagtag agatcgacga ctcattacat gaattgagaa aaggcgaaaa
     8821 agaagcgcaa gaaaagatcg atacattcga attcaaattg cgcagtttga aacgttttgt
     8881 agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta gaattctttt ttgttgctac
     8941 tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca tggaagaggt
     9001 caatcgttta gttgctctat gtgaagaaga tctagaatta ttagataaga agacacatga
     9061 tttagtagat gcagcagcat taacggagca aatgctccaa tatgcaaatc gttatcgtca
     9121 tacacacgaa gaagtgagag aagcaattga taaaagctac tatttattca ataaagaata
     9181 tcactatcaa gaagcattag acgaaatcgg tacagcatta gagcgagtag aacctggtgc
     9241 tttcaaacgc atcgaagatt tttattttaa tcatccagac ttagtataaa gcaaaaaagt
     9301 tagtaaacca gcaagagcaa gacggattat tgtcttgttc ttgtcttttt ttgtatattt
     9361 gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag caccttggac aaccgttata
     9421 atagaaaaga atttcaaaaa agaggggttt cttatgatat attttgataa cagtgcaaca
     9481 accccaatct ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg gattattggg
     9541 aatccatcaa gcttgcatga tttaggcaat caagctaacc ggttgttgca gcaagcaaga
     9601 aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa tctattttac ttcaggcgga
     9661 acagaaggag ataactgggt attaaaagga acgatgatcg aaaaaagaga atatgggaat
     9721 catatgatca tttcaggagt cgaacatccg gccgtcagcg agacagcaga acaattaaaa
     9781 gaattaggaa tcgaagtaag tatcgcccca gtggataaac gcggatttgt acgtgtcgac
     9841 gaattgaaag aattaataag aaaagagaca gtattagtct ctgttatggc tgtgaataat
     9901 gaagtaggat cgattcagcc catccaggaa atcagtgatc ttttggatga ttttcctaaa
     9961 attcatttcc atgtagatgc tgttcaggcg attggaaaag taccattctc gcaatggttg
    10021 acagaaagag ttgattttgc aacattttca gctcataaat tccatggccc acggggaact
    10081 ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat tactgacagg tggagggcaa
    10141 gaaagaaatc agcgaagtgg gacagaaaat gttccaggta ttgtggctat ggttaaagct
    10201 ttacgtttat cattagagat aaaggaacaa aaacctgagc agacacaacg cttaaaagta
    10261 gctctaatca atgcgttgaa gacgtatgag aaagtgacga ttttttcaga aggaactgac
    10321 tatgcaccgc atattttatg ttttgctcta aaaggaattc gcggcgaagt tctcgtccat
    10381 gccttagaag aaaaacaaat atttgtttct actactagtg cctgctcctc acgtaaacat
    10441 atggcaagca gtacattgca cgcaatgcat gtaccaaatg atctggctac atctgctgtt
    10501 cgaatcagct tagatgaaaa caattcgctt gcagaagtcg agcaatttat gattattttc
    10561 aagcagcttt ataagaaatt ttctgcaatc aattaaaaga ggagtgttct tgtgaaatac
    10621 agtgaaatta tggtccgtta tggggaactt tcgacaaaag gaaagaatcg aaaaagcttt
    10681 atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag actttccaga aatcaaaatc
    10741 catgctgatc gggaccggat gcatctttta ttgaatggcg cagacgggca gctagttatt
    10801 ccaaaactag caaaaatatt tgggatccaa aatttctctc caagtattcg tgtagaaaaa
    10861 gatgtcgaag ttttgaaaca agctgttcaa gacatcatga aagaaattta tactgggaaa
    10921 gaaacattta aaattatcgc taaaagaagc gatcaccaat ttgaattgac aagtaatgaa
    10981 ttgaatcaaa cattaggtaa tgctgttttt gatattttcc cgcatatcca agttcaaatg
    11041 cgtcaaccag atattccatt acgtgtagag attcggcgtg atggcgctta tctatcgtat
    11101 gaaacgatca agggagctgg aggattgccc gtaggaacaa gcggaaaagg aatgcttatg
    11161 ctttctgggg ggattgattc tcctgtagca gggtactttg ccatgaagcg gggagtggaa
    11221 attgaagctg ttcattttgc tagtccgccg tatacaagtg aacaagcact gcaaaaagca
    11281 aaagatctaa cagccaaact agcaccttat gtgggaacga tccattttat cgaagtgcca
    11341 tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac aaggatattg gatgacaatt
    11401 actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca tggtttagtc
    11461 attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag tatggtggct
    11521 atcaatgaag taacgaatac gccaatcatt cggcctgtag cgacaatgga taaacttgaa
    11581 atcattgaag tagcagaaca gatcgataca tttaatcttt ctatccagcc ttttgaagat
    11641 tgctgtacga tttttgctcc tccacagcca aaaacacgcc ctcaactgga aaaggtccat
    11701 caatatgaag agcgtttagc aatagatgaa atgatccaac gagcactagc tggcttgaag
    11761 atggaagaga tacaagcaga atcgtctaat gaaaaaactg aagaattttc agaattgcta
    11821 taagaggtta tgaaaatagt gttttgacca gagtattaga gaaacaaaac gaaaaaatag
    11881 ctccatgtat tttaccgttt ttgaactaat gtcgaagatt agctgattga ataccattta
    11941 tcaagaagtt atggaaaagc cggttctttt ttactaactg aaaaataaaa ttttatttag
    12001 agatcaagct acagttgata aatgatataa aaaagagaat ttcccatgaa atagcgaggt
    12061 tgtgacagaa gtgggcagct tcaagaaata agaaggaatt tacgaaaatt gcttttcaaa
    12121 tttttgtgaa atttcggctt atttttcgaa gaagttactt ctggaacacc gtgtatcaga
    12181 ggttgcgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
    12241 atattttacg tttggttgag cttattaccg cagatgcaag cttttgaaca ccgttttatt
    12301 tgtctcacac ccttttcctt cctacttgta accttgctcc cgtgttagaa acatgttaca
    12361 ctaaatatgt gaaattatta acaaggagcg tcgttatgcg agaaaaaaca gatacactgc
    12421 caaaagcaac tgcgaaaaga ttacctttat atttacgcta cttgaagatg ctggatgact
    12481 caggtataag tagaatcaaa tcaaatgagt tcagcgaaat cacccaaatt ccttctgcaa
    12541 cgatccgcag agacttctcg caattaggcg agctaggcag aagcggatat ggctatgatg
    12601 tcccttttct tatcgatgta ttcaataata tccttaatac taaagaagaa aaacgaatcg
    12661 cattagtagg atatggaaat cttggcaaag cattgaaaca caacaatttt agaagaaatg
    12721 aaaatctaaa tatcgtgtgc gtctttgata atgatccagc actgatcaat cgagtaattg
    12781 atggagaaat gatctatccg attgatcgtt tcgcggagat agccaaagca aaaaatgtga
    12841 cagtagcaat ctctacggta ccaagtaaat attcacaatc tgctattgat gaaattgtca
    12901 aaggaaatgt aaccgctata cttaattttg cgcctgacag agtaactgtc ccagcttatg
    12961 tcaatgttca atacattgat ttgacaaccg agttgcagac gcttatttac tttgatgaga
    13021 attactcaga agttttttct taggaaaatt tgagtaggct tattgatctt gcgagaactc
    13081 gtcgtaattc tttcaaaaat tttttagtac taaatgaagt ggttttttca cgaccacttg
    13141 ttgactaaaa aaaaaaacca ttgtagaatg aagcataacg ctgaacaaga ggagtaactg
    13201 gcacattcat tcttagagag agaactatct gctgaaaggt tctcaatgac aaccagtgaa
    13261 ggtagcttgg gagtttctgt cttgaatgga gtagagatag acgagtcgtg atcgttaact
    13321 cacttgagag gcaataatga ttgattattg caatttaggt ggtaccgcgt aaatcatacg
    13381 tcctagaata cgaaagtatt ttaggacgtt ttttagttta aaggaggaaa acagcatgac
    13441 ggaagaaaat aacctatcaa caaaatataa tccgcaagaa gttgaagctg gacgttatca
    13501 gcattggtta gaacaagatt tgtttaaacc gagcggagat aaaaaagcaa aaccttattc
    13561 aatcgtaatc ccgcccccaa atgttactgg aaaacttcat ttagggcatg cttgggatac
    13621 gaccttgcaa gatatgctga ttcgtcaaaa gagaatgcaa ggatatgata ctttatggct
    13681 gcctggtatg gaccatgcag gaatcgcgac acaagcaaaa gtagaagcga agcttagaga
    13741 gcaagggatt tctcgttatg atctaggtcg tgaaaagttc atcgatcaag tgtgggaatg
    13801 gaaagaagag tatgcgagcc acatccgcga acagtgggct aaaatgggct tatctttaga
    13861 ttacagtagg gaacgtttta cattagatga tggattatct gaagcagtac gtaaagtttt
    13921 tgttacctta tacgaaaaag gtttgattta tcgtggtgaa tatatcatca actgggatcc
    13981 tcaagcacga acagctctat ctgatatcga agttattcat aaagatatcg aaggcgcctt
    14041 ttatcatatg agctatgaac tagcagatgg atcaggcgta gtagaaattg cgacaacccg
    14101 tccagaaacg atgttgggag atacggcgat tgctgttcat ccagaagacg agcgttatca
    14161 agcgctcatc ggcaaaaaag tgattctacc tttagtcaat aaacaaatcc caatcattgc
    14221 agatgaatat gtagatatgg aattcgggac aggtgtagta aaaatcactc cagcacatga
    14281 tccaaacgac tttgaggtag gtaatcgtca tgatcttcca cgcgtaaatg ttatggatga
    14341 tgatggtacg atgaatgaac tagcaggaaa atatgctgga atggaacgtt ttgctgctcg
    14401 taaagcaatc gtgcaagatc tgaaagaaat cggtcgattg attaaaatcg atacgatgat
    14461 ccacagtgtg ggtcattccg agcggacagg tgtcgttgtt gaacctcggc tgtctactca
    14521 atggtttgta aaaatggcgc cgttagcgaa aaaagccatt gataaccaag aaacagatca
    14581 agcagtggaa ttcttccctc caagattcaa tcaaacgttt ttacgttgga tggaaaatgt
    14641 ccatgattgg gtaatctcac gacaattatg gtggggacat caaattcctg catggtatca
    14701 caaagaaaca ggtgaaatgt atgtagggat ggaagcacca gcggatagtg aaaactgggt
    14761 acaagatcct gatgttttag acacttggtt tagttcagct ttgtggcctt tctcaacaat
    14821 gggctggcca gatacggaaa gcgaagatta taagcgatac ttccctacta gcactttagt
    14881 aaccggttat gacatcattt tcttctgggt aagcagaatg attttccaaa gcttggaatt
    14941 tactggcgaa cgtccatttg agaatgtctt gattcatgga ttgattcgtg acgaacaagg
    15001 gcggaagatg agtaaatcat taggtaatgg aatcgatccg atggaagtaa tcgaaaagta
    15061 tggagcagat gctctccgct ggttcttatc gaatggttct gcaccaggac aagatgtccg
    15121 cttcagctat gaaaaaatgg atgcttcttg gaactttatc aacaaaatct ggaatgcttc
    15181 acgtttcgtt atcatgaata tcgaaggaat gaccgtagaa gagatcgatt tatctggaga
    15241 aaaatcagtg gctgaccggt ggatccttac tcgtttgaat gaaacgatcg aacgagtaac
    15301 tgagctattt gatcgttttg aatttggtga agcaggtcga caactgtata actttatctg
    15361 ggatgatttt tgtgactggt acatcgaaat gagtaaagaa gtactttatg gagatgatgc
    15421 tgtttcaaaa caaaccaccc gtagcatctt agtccataca cttgatcaga tcctacgttt
    15481 attgcatcca atcatgccat tcgttacaga agaaatatgg gaacatattc ctcatcaggg
    15541 aaattctcta gtagtggcag aatatcctgt tgtacgtcca gagtttgatg atgaaacagc
    15601 ttctaaaggg atggaagtat tgaaagaatt gattcgagcc gttcgaaata ttcgttcaga
    15661 agtgaataca ccattgtcta aaccaattac gttgctaatt aaaacaaacg atgctaaaat
    15721 cgatcaattt ttagttgaaa atacaagcta tatcgaacgc ttctgtaatc ccgaagagtt
    15781 gacgatatca agtgagatca ctgcgccaga tctagcgatg tcagctgttc tgacaggtgc
    15841 agaaatcttc ttgccgttag caggattgat caatattgaa gaagaaatca aacgattaga
    15901 aaaagaactg gaaaagtgga caagcgaagt aaaacgtgtc caaggaaaac tttctaatga
    15961 acgttttgtt tctaacgcgc cggaagaagt agtagaggca gagcgtgcaa aagaaaaaga
    16021 ttatttagag aagcaaacgg ctgtcaaaga acgaatcgaa agtttgcgta ctattcaata
    16081 agaatagaaa aagagagtgt gaaatcagct agctattact aaaagctagc tgattgaaca
    16141 tcgttacagg aaagagtttg agcctcactc ttagcttcga ttcataagga gatgtgacat
    16201 aaattttgtc gcatctcttt tttagtctac tcttattatg taggagaagg agggaggtgg
    16261 atcatgtatc aagagtcaat aaagtagttg ataaaatcgt tcctctctct gtttcttttc
    16321 tctttgtcat acttttattt ttcttatgtg ttctatttgt tataataaaa gagaaatggg
    16381 ggaattgttg atgacgattg aagaagcaat agaatggatc catagtcgat taccatttgg
    16441 ttcacgtcct ggccttgacc gagtcgaagc tttattagaa gtggtagggc atccagagag
    16501 aaaagtaaaa acgatccata tcgctggaac aaacggaaaa ggttctactg tagcttattt
    16561 gagaagccta ttagaagagg ctgggctgac agtaggtaca tttacttcac cttatatcga
    16621 aacgttcaat gaacggatcg caatcaatgg caaaagcatt ccagacaatg atttgatcca
    16681 gcttgtaaaa aaatatcagc cactgatcaa acaacttgat caatttgaag cagtagctgg
    16741 tattactgaa ttcgagacat taacagcaat ggcatttgat tattttttga tgaagaaagt
    16801 ggacgtagcg attatcgaag ttggcttagg cggcttattg gattctacca atgtagctgt
    16861 accaatgctg acgggaataa caacgattgg acttgatcat accgaaatac ttggagatac
    16921 aattgaagaa atcgctgcac aaaaagctgg aatcatcaaa caaggcgttc cagtagtaac
    16981 aggaaatatt gccccagaag cttttactgt catccaacaa acagctaaag caaaaaatgc
    17041 accgctttat gtatacgggg aaaattataa aataaactat ctgcatcctg atgagatgtg
    17101 gggagaagta tttgaatttt ttaatgaaat aggaaagatc cctcaattaa ccactccgct
    17161 tttagggcaa catcaagctg aaaatgcagg agtagccatc gaattgtatt ttgtttactg
    17221 tcaattggaa aagctgcctt tccgagaaaa agatgttttg aagggattga agaaatctcg
    17281 atggccagga agaatggagc gtttgagtaa cgagccatta gttgttttag atggtgccca
    17341 taatacgcat gcaatgaacc ggcttgttga gaatttgaaa aaagaattca aaggatatca
    17401 aatcaacatt ttgttttccg ccttacgaac taaaaatgtt agagaaatgc tagaacgatt
    17461 gcagcaagta ccaaatgtcc atatttattt gacgaccttt gaacatccaa aagccatcga
    17521 attaagtgaa gtatcagatt tagcaagtga gagagtatca atcgtttcat tgtggcaatt
    17581 cggattagca gatatattgg aaaagatgac aaatgaagac gtattagttg tgaccggttc
    17641 actttatttc atttcagaag ttcgccattt attaatacaa ttaggaggag atcatgaagt
    17701 ataatgcagt gatttttgac atggatggtt tgctgttcga cacagagtta gtgtattacg
    17761 aagccacaca agtagtagca gacaagatgg gttttcctta tgataaagac ctgtatttaa
    17821 aattcttagg tgtttcagat gaagaagtat gggcgaatta tcatatgatt ttctcggaat
    17881 ttggcaaaac aagtgtacaa agattcatag atgattcata ccaggaaacg cttgaacgtt
    17941 tttcgcaagg agcggtacaa atgaagcccg gtgtgttgga actcctctct tttttagaag
    18001 aacatcagat tcccaaagtt gtcgcttcca gtaaccaacg agcagtcatt gaacttttat
    18061 tgaagaagaa caatctgttt gaacgatttg agacgattgt ttctgcagaa aatgttcaac
    18121 gagcaaaacc agacccagaa attttcttgt tagctaatca gaagctaggt acagcgaaga
    18181 aagaaacatt gattttagaa gattcacaaa atggtatttt agcagcttct gcggctgaga
    18241 tcccggtagt catggtacca gatctgcttc caccatccca agaattgatc aaaaaaacag
    18301 ctggggttgt ttcttcttta catgaaatcc ctgcattgtt aaaataaaaa tccatctctt
    18361 ttcgtatctt attttagata agatagaaag gagatgtttt tttgggaaaa cgattgatca
    18421 aagaagtacc tactagctct ttaccaaggg aacgtatgga aatttatggc gcggaagctt
    18481 tatcggatca ggagcttttg gcaatcttac tgcgtaccgg tcaacaccct tatagtgtga
    18541 tgtcgattgc tggaaatctg ttaaaaacgt ttggaggact ggcctcttta cgacaggcta
    18601 ctttgcatga gttagaagag attcgaggga taggacgcgt gaaagcaatc gaaatcaaag
    18661 ccttgatcga attaggtcga cgaattcaga cggatcatga aagagcggct ccaccggttc
    18721 gtaccagcta cgaattggcc cagcaattga ttctggaaat gaaagatcac aaacaagaac
    18781 atttggtttg tatttatttg gacacgaaga atcaagtgat cttaaaaaaa acagtgttca
    18841 tcggttcgct gaatcagtca gtcgctcatc ctagagaaat tttccattat gctgtacgtt
    18901 attgtgcagc aaggatcgtt ttagcgcata atcatccttc tggtaatgtt attccatcgc
    18961 agcaggatat gaattttacc aaaaggatac aaaagtgtgg agaaatgatg gggatcactg
    19021 tactggacca cttgatcatc ggacgcaaac gatattttag tttgagagaa gaaggaatga
    19081 tggaagagaa gtaagttgct ttcttgcaaa aaaaatgaag caagtgtaca attatattgt
    19141 aattttgttt gggaatagaa gttgaaacat ctgttgcacc tctatcgtac acgagcaatg
    19201 ttacaattta aattagtgct tttggcacga ggaggatatt attatggaaa acggaacagt
    19261 aaaatggttc aacgctgaaa aaggatttgg atttatctca cgtgaagatg gaagcgacgt
    19321 attcgttcac ttctcagcta tccaaggtga cggattcaaa acattagaag aaggtcaagc
    19381 agtgactttc gacgttgaag aatcagaccg cggcccacaa gctgcaaacg ttgtaaaagc
    19441 ttaattttat ctgtaaatca aattgatggt agacatcaat ttttacgaga gaacaaagcg
    19501 agtaacaaaa atacctgcca caagagacaa tttctcttgt ggcaggtatt tttttgtatg
    19561 aaaaagtaaa taaatttaag aggctatgaa agagccacct tgtatcaagg aataagaaca
    19621 aacaaaaatg gcttctcatg tttttcattt gttttctctt atattcagga caaggtgctg
    19681 atttcataac ctcttattta ttcttcatct ggtgtcatta ccattggcaa aatcattgga
    19741 tgtctttctg tacgttcgaa taagaaaggc tgaagcgcgc cagtcatcgc ttctttcaat
    19801 ttatattctg aacagtcagg tccattcaaa cattctctta aagcgtggaa taagattcgc
    19861 tgagcttcat tgatcatttc tcctgattct cgcatgtaga caaatccacg agagagaata
    19921 tctggaccag ccaagatttc ttttttcttg atatccactg tcgcgacagc taatacaaga
    19981 ccttcttctg aaagcaggtg acggtcacgt aaaacgacat taccaatatc accgatcccg
    20041 ctaccatcga tatacacatc tccagccaca aaatgcccag caggacgcgc agaatcgcta
    20101 gttaacgcta aaacatcgcc attttccatg atgaagcagt tttctgcagg aacacctaca
    20161 tcttgtgcta gagaagcatg tactttcaac atgcggaatt ctccgtggac aggaataaaa
    20221 tattttggct tcatcaaacg aagcattaat ttttgttctt cttgtccacc gtgaccggaa
    20281 gtatggatgt tattgatttt tccgtgaatc acttctgcac cagcttcaga cagcaaattg
    20341 atcaaatgat tgacacttgt tgtattacca ggaataggtg aacttgaaaa gatcactgta
    20401 tcgtctggct ggatttggat ttgacggtga gtaccattcg cgatgcggct caacgcggcc
    20461 atcgcttctc cttgagaacc tgtacatagg atcatcgttt cattggcagg caagcgattg
    20521 atttcgtgtg catctacaaa tgtgccgtct ggaactttga tatatcctaa acgttgaccg
    20581 ttgacgatgg cattttccat gcttcgtccg aaaacagcga ttttacgacc agttgccaca
    20641 gcggcatctg ctgcttgctg caaacggaag atgttagaag caaagctggc aaaaatgata
    20701 cgtccttcga tttttgaaaa gatcttcaaa atagaatgcc caatcgtttt ttctgattta
    20761 gtaaatgttg gaatctcagc atttgtacta tcagataaaa ggcacaagac tccttcttcc
    20821 ccgatacgag ccattttatg caagtttgct ggttcgccaa caggagtgaa atcgaatttg
    20881 aagtctccag tcgctacgat atttccagaa ggtgttttca ccacgatccc taatgcatct
    20941 gggatactat gggtcgttct gaaaaagctg acagaagtct ttctaaaacg gatgatcgta
    21001 tcttcattga tctcatggag ctctgcatct cgaagcaagc catgctcatc taatttgttt
    21061 gtgatcaacg ctaatgcgag aggaccagca tagataggaa tatttgcttg tttcagcaag
    21121 tagggtattc cgccgatgtg gtcttcgtgc ccgtgagtaa tcaccaaggc ttttactttg
    21181 tgtaagtttt gtatgatgta gctgtagtcg ggaatgacat agtcaattcc aagtaaatcg
    21241 tcttccggga atttgatccc ggcatcaatc aaaatgattt catcttggaa ttgtacccca
    21301 tacgtatttt tcccgatttc acctaatcca ccaatagcaa agacgcctgt ctcgttattt
    21361 ttgatggaaa gtttcatatt aaaactccac taatgagaaa tcagcatttt ctttttcgta
    21421 ctctagatga cttccttcta gaagttgaac gaattcaata ttgtatgggg tattttcttc
    21481 cacacgttgg cgagcgtcta catcgctttt tgcctcaatg taaagagatt ttgtttcttc
    21541 ccgtttaggg ttgcgtactt ttgtttcttg ataataaact ttatagatca ttgttttgtc
    21601 tcctttatct gcgtaaagca gtgatttagc tagttgattt ttttagacaa cacaaaacga
    21661 ggggacaagc cacctggttt ttcatatgct ttgactagaa tactggattc gagtcaaaga
    21721 gacaagcctc gtttttgctt atctgttcgg atattttttc aaccgtacct tctgtataaa
    21781 aaatatacaa caattaattg ttattctagc ataaaacagc aaataagtat agaaatcaac
    21841 tagattgaaa gaaaaaatca atgtttatca tcccgctata atcataatga cttttttatg
    21901 agagaagtgt tctattaata ggctcgttgt gctatcattt ttagtaatga aaatgtaaca
    21961 aaagattaaa aggagatgag aaagtgaaac taatgtggca ttataccatg cgttataaga
    22021 aattcttgtt tttcaatttc atatgcgtat ttggttttat tttgattgaa ttaggacttc
    22081 ctacgatttt agcacgtatg atcgatgtag gaatccgcaa tgatgatttt gattatgtga
    22141 agcaacaagg gatatttatg atcgttatta ctgtaatcgg tattattttg aatatcttgt
    22201 taggttattt tggttcgagg attacgacta atatcgtagc agatattcga gatgatttgt
    22261 tcgaaaagat ccaaagttat tcgcatcagg aatacgaaaa tctaggcgta tcttcattga
    22321 tcactcgtac aacaaacgat gcttatcaga tcatgttgtt cctgcaaaat attttacgta
    22381 tcggttttat ggcaccaatg atgttcattg tcagtttgta catggtcatg cgtacaagtg
    22441 tgacactcag tttgtatgta gtaggtgcat tacctttact attattggca gtcgtagcga
    22501 ttgctaaggt gtctgaaccg ttatctaaaa agcaacaaaa aaacttagat aaaatcaaca
    22561 gtatcttgcg ggaaaatctt tcaggattgc gtgtgattcg tgcattcgtc aatgaaaagt
    22621 ttgaagaaaa acgttttgct gtagtcaatg agaattatgc ttctagttca aaaagcttat
    22681 tccgtttgat ggctgttgca caaccgggtt tttatttctt atttaatatc gtaatggtat
    22741 tgatcatctg gagcggggca gtgcaaatcg atgcaggcga gcttcgtgta ggagatttga
    22801 ttgcgtttat cgagtatata ttccatgcct tgttctcatt catgttattt gctagtgtgt
    22861 tcatgatgta tccaagagca gctgtttcag ctggacgtat ccaagaagcc ctagatgcca
    22921 aaccttcgat tgtagaagat ccaaatggta tatctgatac aaaaactaaa gggtatatcg
    22981 aatttcaaaa tgtaacattt gcttatccag gccattcgca agagccagtg atacgaaatg
    23041 tcagttttac agcctctcca ggagagaccg ttgcttttat tggaagtacc ggtagcggaa
    23101 aatctacgtt gatccaattg atccctcgct tttatgatgt gagtgaagga aagatcttga
    23161 tcgatggagt agacgtgaga gattatcggc taagcaagtt gcgagataag attgggttta
    23221 ttccgcaaaa agcgctgcta tttactggaa cgattgcaga taatttgcga tacgggaaag
    23281 aagatgctac gcaagaagaa atggaacgtg cagctgagat tgctcaagct tcagaattca
    23341 tttctcgaaa accagatgga tttaatgaac atttatcaga aggtggttcc aatttttcag
    23401 gtggacaaaa acaacgattg gcaatagcca gagccatcat acgccgtccg gaaatctata
    23461 tttttgatga cagcttttct gcattagatt atcaaacgga tgcgaagtta cgtgctcgtc
    23521 tgaaaaaaga aacgacagaa tctactgtat tgatcgtagc acaacgtgtg ggaacgatca
    23581 tgcatgcaga taagatcatc gtattaaatg aaggagaagt cgtcggtatg gggacgcatc
    23641 gggagttgtt ggaaaaatgc ccgatttatt atgatattgc cgcttctcaa ttatcaaagg
    23701 aggaattagc atgaaaaaaa ccttttcttc cttaaaacgt ttagctcgat atatcagacc
    23761 gtatcgtgag acttttcttt tagtgatcct atttacagtt ctgacagtgg ctttcaacgt
    23821 ggctttgcca tatgttacag gacttcctac tacagaaatc agccgtaata ttgccaatgg
    23881 cgaatcaatc aatttttctt atatattcaa atgtctcatt tggatcacag ttgttggaat
    23941 agggtattgt atttcgcaat tactttctgg tgttttgatg acaaatgtcg ttcaaagtgc
    24001 catgcatgat ttgagaagag acatagaaga aaaaatcaat cgtcttccgg tttcatattt
    24061 cgataaaaac cagcaaggaa atattttgtc tcgtgtgacg aatgatgtag atgcagtcag
    24121 cggtgcttta caacaagctt ttattggtat cgtaaatgcg atattgggaa ttacgttggc
    24181 tgcagcaatg atgttttata tccagccgat gatggcgctg atctctatga tcatgattcc
    24241 aacatcgatc tggatctcaa aacgagtgat caatgcttcg cagaaatatt ttcaaagcat
    24301 gcagaattct cttggtgagc taaacggcta cgtccaagaa aatatgacag gttttagtgt
    24361 attgaaagta tatggacgcg aaaaagaaac attggaagga ttcaaaaaag ttaatcacag
    24421 cttgaaatac tttgggtttc gtgcagcatt catttctggc ttgatgatgc ctctagttca
    24481 attaactgcc tatgctactt atattggtat ggctgttcta ggaagctttt atgttattac
    24541 tggggcgatc gttgtcggtc aactacaagc attcatccaa tatatttggc aaatcagtca
    24601 gccaatgggg aacgtgacac aattgtcttc agttttgcaa agtgcatcag cggcaacgaa
    24661 acgtgtattc gagattttag atgagccaga agaaaaagaa aacaaagtag atgcttcact
    24721 gccagaaacg attttaggag atgtgacatt cgatcatgtc gattttgcat atgatccgaa
    24781 gaaaccatta atccaagatt tgaattttga agtgaaagct ggtcaaaccg tagctgtggt
    24841 cggtccgacc ggtgccggca agacgacatt gatcaatcta ttgatgcgtt tttatgacgt
    24901 agacaaagga gcaataagga ttgatggaat cgacaccaaa tcaatgagtc gcagtgatgt
    24961 gcgttcgcta tttggtatgg tcttgcaaga tgcatggctt tatcaaggaa cgattgcaga
    25021 taatatccgc ttcggaaagc ttgatgcgac agattatgaa gtagttgatg ctgcgaaaac
    25081 agcaaacgta gatcatttta tccgaacgat gccagatgga tatgatatgg ccatcaattc
    25141 tgagggagac aatgtgtctt taggacaaaa acagttgctg acgattgccc gagcagtggt
    25201 ctccgatcct aaaatcttga ttttagatga agcaacaagt tcagtagaca cccgtttaga
    25261 agcgttgatt caaaaagcaa tggatcgcgt gatggaggga cggacaagtt tcgtcatcgc
    25321 tcaccgttta tcgacaatcc gtgatgcaga tctgattcta gtgatggatc aaggtcagat
    25381 cattgaaaaa ggaacgcatg agagtttatt ggcacaaggt ggattttatg aaaaactgta
    25441 taacagccaa tttgctgagg aagcagaata agtcatataa gaataaaaca gtttaaaact
    25501 tattttttgt tctctattct tgataatggt aaagtaaagt tatcaagaaa agtgaggaca
    25561 cggagatgga acagttttta aatgaaaaaa ttactgaagc caaaagagaa gttgatcgtt
    25621 taacgcacgt ctgtcagacg aagccttttg attcagctga tttttttgaa aatgagtttc
    25681 aaatcgaacg cttggcagca caaatagaag tgtacgaatt aattttaaaa aaagtaatag
    25741 aagaaaatat agatgtacaa taaaaacagg taaaacgtca gttaccagat actgtaaata
    25801 gacatctggc ctgagcaggt tttacctgtt ttttttctta tgaaataaat atatgctatg
    25861 atacctttat tcaataggat tagtttctac aaaaaatgaa aggagaaaaa taaatatgga
    25921 acggtttata gcatcaaatg ccacagtgat tggtgatgtg acattgtcag aagatgtgac
    25981 gatctggtat caagctgttt tacgcgggga cagtaattgg ataaaaatag gacaaagaac
    26041 gaatatccaa gatggtacaa tcatccatgt cgatcatgat gcacctgtag atattgcgga
    26101 gaatgtgacg gtaggacatc agtgcatgct acatggctgt acaatcgaaa aaggggcgct
    26161 gatcgggatg gggaccacga tattgaatca tgcggttatt ggtgagaata gtttgattgg
    26221 tgctggatca ttagtaacag aaggaaaagt tattcctcct aatgtcttgg cttttggtcg
    26281 cccagctaaa gtgatccgtc ctttgacaaa agaagaaata caaaaaaaca aagaaaacat
    26341 ccagcattat gtcgagatag gtcggatgca tgcgcaagga aaattcaagg agcaaagata
    26401 ggcagtagca tccataaaat aaaaaaggat ggtcagtttg agttgaccat ccttttttat
    26461 ttggtttaga taagagagct taaaaaagct tcctttgaaa cagattaatt taatgaagaa
    26521 ctgttttcca tgacttcatc gatcaatcca tatgcttttg cttgttcagc tgtcatatag
    26581 ttgtcacgat ctgtatcttt ttcaatcact tcaagcggtt gtccagtgcg ttcagctaaa
    26641 attttgttta agcgttcgcg tgtttgcaaa atatggcgtg ccgcaatttc gatttcagtt
    26701 gcttgccctt gagcgccgcc aagaggttga tggatcatga tctcagcatt tggtaaagca
    26761 aatcgtttac cttttgtacc agctgtcagc aagaagcttc ccattgaagc agccataccc
    26821 ataacaattg tttggacatc tgctttgaca aagttcattg tgtcgtaaat agccatacct
    26881 gctgtaactg aaccaccagg agaattgata taaagataga tatctttttc agaatcttga
    26941 gcgtctaaga aaagcaattg ggcgatgatc gaattagcaa gatcatctgt tacttggccg
    27001 cttaacatga tgatacggtc tttcaataaa cgtgagtaga tatcgtaagc tctttcacct
    27061 cgtgaagatt gttcaatgac tgtaggaatt aaattcataa tagagttcct ccttgatttc
    27121 gtttagaaat agtgtaccat aatttagttt ctaataccta aaatgtagta tacatttttg
    27181 gtcaaaaaag gtcaaataaa aaacttaggc aaattagtcg cataaacccg ctttatggta
    27241 tgataagcag agaaacaaaa caaggaggaa caacagattg atagttggta ttactggcct
    27301 tatcggagtg ctgacaggtg ctgtagttgt ctatatcggc atgatactgc aaatgcgtca
    27361 cttggaaaat aaagaacagc gtagacgtga attggaattg aaagtcaaag aaatcgagac
    27421 gctgaatcaa ttaaataaaa agatcaacga gatcttgcaa aaacggacaa cgctgatgga
    27481 agaatatgtc agctttgatg catttgatga ttgttatatc acgattgacg attttgcgta
    27541 tctccaatca ttttcagcgc aaaatagttt ttatctgcca aattatattt tagacgagct
    27601 atttaagaat atctcccatc gaaaagtgat tttatctcca gatgaaacaa tgaagattgg
    27661 tggatatgct tataaaggcg gacgcgtgat tttagagaat tttacggata gtctgactga
    27721 aatgatcaat gaaaaaaaag ctcagttgaa gactatgacg cgccagccga ttggtttctt
    27781 ctcgaaagat gatgtttgga gctgatattt tgaagcattg ccgttgcaca tgagagcgtg
    27841 gtaatgcttt ctttttttct taatgataca atagaactga acggagtgaa gaaagatgtt
    27901 acaaatctat gcccatcgcg gaagcagcgg cacccaccca gaaaatacgc ttcctgcatt
    27961 tgcagaagct gtacgagtag gtgcagatgg tatcgaacta gatgtccatc taagcaaaga
    28021 tggttatttg atcgttatgc acgacgaaga agtggaccgg acgactaacg gtaaagggct
    28081 gattcgagaa aaaacattgg aagaactcaa aaaattgaat gcagggggca aattcagcaa
    28141 agaatttcca gcagctaaag ttcctgaatt gagagaagtg ttaggactgc tcctccaaaa
    28201 aaattaccga gggatgctaa tgattgaaat taaaactgat cagtatgaat atcccggtat
    28261 cgaagaaaaa ttagccgaaa cattaaaaag tagagattgg ccttttcaac atgcatattg
    28321 cagctttcat ttggattctt tggaaaaaat cgcattactg gaaccggaga tacagctgga
    28381 cttattgatg agtacttctg ataaaaagat ccaactagca gcagaaaccc cttatataga
    28441 aggagtacat ccaaaaaata cttggttgtt cgaacatcca gtccaggcag ctgattttcc
    28501 aaaagcaatt cgtgcatgga cgattaacga tgaagaagaa atggccaaat gtcaagaact
    28561 caaaatagat ggaataataa ctgattttcc ggagaaagct cttcggtgga aacaatcaac
    28621 gaaaagtagg taaaatatgg aaaaagtatt agtgatcgtc ggtccgacag cagtcggtaa
    28681 aacagcgcta agtgtggaac tcgcacaact atttggagga gagatcatca gtggtgattc
    28741 attgcaaatc tataaaaatc tggatatcgg tacagcaaaa atcaaagaag aagagatgaa
    28801 taatgttccg catcatttga ttgatgtgat cgaacctact caacgttact cagccgctga
    28861 ttttcagcat tcaggtcgac aattgattac cgagattgcg aaccgagggc acctacctat
    28921 catagctggt ggtacgggtc tctatatcca atcccttcta tatgattatc agttaggagc
    28981 gacagaagaa gcgaatcctg agattagaaa aaagtatgaa acgttagctg aaacaattgg
    29041 ttcccaagca ttatggaatc agttgaaaga aaaagacccg gcagctgctg agaaaatcca
    29101 ctggaacaat caacgcaaag tgataagagc tctggaagtt ttagagacga caggacatag
    29161 tatcacggca cctaaagaag agccaaagca attgtatgat tacctcatga ttggattaga
    29221 tacgaagcgt agtattcttt atgaacgaat caatcaaaga gtcgatttga tgctagcaga
    29281 aggtttagta gcagaagcgc gtaaggtgta tgagcttgga gaagtccagg ccagtcaagg
    29341 tattggatat aaagaatttt tcccttattt tgaagggaaa gaaacattgg aagaagcaac
    29401 agaacaagtc aaactacatt cgagaagata cgcgaaacgt cagttgacgt ggtttcgtaa
    29461 ccgtttggat gcacagtggt ttgatcttat aagtgagcca aatcaaaaaa gtcagattga
    29521 gcaaacaatc aaaaaatggc tggaggcgga ataaaatgga agaaaaagtg atcatcgtag
    29581 gggtagaaac cgaagcaaac cagcgttact tttcagagtc aatggaagag ctagttcagc
    29641 tgacgaatac cgcatcagga gaagtcgtat tcacaattac acaaaagaga ccacaagtgg
    29701 atcggcaaac gattatcggt aaagggaaat tacaagaatt agttcaacaa gcggatgcgc
    29761 atgaagcgga tttgatcatc tttaatcatg agctaactcc gagacagagc caactgatta
    29821 ccgatgctgt tggtcttccg gtaattgatc gcgtgcagct tatcttagat atttttgcga
    29881 tgcgtgctcg ttcgaaagaa gggaagctgc aggttgaact tgctcagtta gaatacctgc
    29941 tgcctcgttt agtagggcaa gggaaaacat tatctcgtct tggcggtggg atcggaacaa
    30001 gagggccagg tgaaacaaag ttagaaacag accgccgaca tatacggaat aaaatttttg
    30061 ctgttaagaa agaattgaaa gaagtagaag ctcatcgaga gcgcaatcgc caaaaaagaa
    30121 aaaatagcga gatttttcag attggactta ttggctacac gaatgccggg aagtcaacta
    30181 tcttgaatct attgacacaa gcggatactt attcgaaaga tcaattgttt gcaacgttag
    30241 atccattgac taagagatgg cgttttgctg aaggatttga gattactgta acggataccg
    30301 tcgggttcat ccaagacctg cctacgcaac tgatcgatgc tttccattct acattagaag
    30361 aaagtcaaaa tatggatctt ttgctacatg tagtcgatgc tagttctcca gatcgtattt
    30421 tacaggagca gactgtctta aaattgatgg atgaactgaa tatgaaagaa atgccgatac
    30481 tgactgttta caataaggca gaccagatcg acccagcgat gttcacgcct acgctattcc
    30541 caaatgtatt gatttctact cagacgaaag aaggtaagat ggcgttgatc gaagcaatca
    30601 aacgtcagct gatggaacta atgacccctt atacactttc tgtgccaagc aacgaaggac
    30661 agaaattaag cgagttgaga agacaaacaa tggttttgga cgaagcgtac atggaagaaa
    30721 caaacgaata tgaagttaaa gggtttgcaa aaaccaattc aaaatggtta agaaaaaacc
    30781 aataaaattt tattttttta acaatattat ttgtcaaatt aagcgagaca aaactttcca
    30841 tttacgtaac tcaaaaaaac ttctaatggt gtttggtaat ttagtgattt tctagggata
    30901 tggtttcttt tggacgcaac ggatgagatg aa
//
LOCUS       DAZKCY010000024        30475 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000024 DAZKCY010000000
VERSION     DAZKCY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.024"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..3664)
                     /gene="rpoC"
                     /locus_tag="ACG8XC_000982"
     CDS             complement(11..3664)
                     /gene="rpoC"
                     /locus_tag="ACG8XC_000982"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP9190697.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3743..7360)
                     /gene="rpoB"
                     /locus_tag="ACG8XC_000983"
     CDS             complement(3743..7360)
                     /gene="rpoB"
                     /locus_tag="ACG8XC_000983"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9190698.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8088..9056
                     /locus_tag="ACG8XC_000984"
     CDS             8088..9056
                     /locus_tag="ACG8XC_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9190699.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9074..9979
                     /locus_tag="ACG8XC_000985"
     CDS             9074..9979
                     /locus_tag="ACG8XC_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9190700.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9976..10782
                     /locus_tag="ACG8XC_000986"
     CDS             9976..10782
                     /locus_tag="ACG8XC_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190701.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10939..11907
                     /locus_tag="ACG8XC_000987"
     CDS             10939..11907
                     /locus_tag="ACG8XC_000987"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFP9190702.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12224..12748)
                     /locus_tag="ACG8XC_000988"
     CDS             complement(12224..12748)
                     /locus_tag="ACG8XC_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFP9190703.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13035..14732
                     /gene="scm"
                     /locus_tag="ACG8XC_000989"
     CDS             13035..14732
                     /gene="scm"
                     /locus_tag="ACG8XC_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFP9190704.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSS
                     TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDN
                     RTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(14887..15873)
                     /locus_tag="ACG8XC_000990"
     CDS             complement(14887..15873)
                     /locus_tag="ACG8XC_000990"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP9190705.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(15896..16462)
                     /locus_tag="ACG8XC_000991"
     CDS             complement(15896..16462)
                     /locus_tag="ACG8XC_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFP9190706.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16476..16892)
                     /locus_tag="ACG8XC_000992"
     CDS             complement(16476..16892)
                     /locus_tag="ACG8XC_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFP9190707.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17349..19283)
                     /locus_tag="ACG8XC_000993"
     CDS             complement(17349..19283)
                     /locus_tag="ACG8XC_000993"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9190708.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19585..20520
                     /gene="pplA"
                     /locus_tag="ACG8XC_000994"
     CDS             19585..20520
                     /gene="pplA"
                     /locus_tag="ACG8XC_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFP9190709.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20610..21713
                     /locus_tag="ACG8XC_000995"
     CDS             20610..21713
                     /locus_tag="ACG8XC_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFP9190710.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21726..22679
                     /gene="menA"
                     /locus_tag="ACG8XC_000996"
     CDS             21726..22679
                     /gene="menA"
                     /locus_tag="ACG8XC_000996"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP9190711.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(23493..24839)
                     /gene="gor"
                     /locus_tag="ACG8XC_000997"
     CDS             complement(23493..24839)
                     /gene="gor"
                     /locus_tag="ACG8XC_000997"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP9190712.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(24973..26226)
                     /locus_tag="ACG8XC_000998"
     CDS             complement(24973..26226)
                     /locus_tag="ACG8XC_000998"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP9190713.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(26232..27155)
                     /locus_tag="ACG8XC_000999"
     CDS             complement(26232..27155)
                     /locus_tag="ACG8XC_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP9190714.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(27376..29868)
                     /locus_tag="ACG8XC_001000"
     CDS             complement(27376..29868)
                     /locus_tag="ACG8XC_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFP9190715.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(29875..30342)
                     /locus_tag="ACG8XC_001001"
     CDS             complement(29875..30342)
                     /locus_tag="ACG8XC_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFP9190716.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         8811 a         6478 c         5699 g         9487 t
ORIGIN      
        1 tttaaaatat ctattttgtt tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa
       61 tatctgaaat actgtagaca ttttcgcttg ctacaccgac ttcttttggt tccatgtcac
      121 gataacgttg cataccagta ccagctggga tgatcttacc tataataaca ttttctttca
      181 aaccaagaag aggatctttc ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt
      241 cttggaatga tgcagcagat aagaaactat ttgtttctaa tgatgctttt gtgataccta
      301 gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac tagcgtattg tagttttgat
      361 ctttaaagtc tgcgatgtct aataatgtac ctggtaagat atctgtatca cctggatcca
      421 ttacacgaac tttacgcagc atttgacgaa ccattacttc gatatgcttg tcgccgattt
      481 ctaccccttg catacggtaa acacgttgta cttcacgaag caagtagttt tcaacagata
      541 atacatcgcg aacttgcaac aattgttttg gatcgattga tccttctgtc aatggtgcac
      601 cacggtggat catatcgcct tctgctactt tcatacgtgc tgtataagga acagtatacg
      661 tacgtgtatc tgtttttcct ttgattgtta cttctttggt acgtgttgca ggatcttctg
      721 agatatcgat gacatcccct gttacttctg tgatcactgc ttgccctttc ggattacgtg
      781 cttcaaagat ttcttggaca cgaggaagac cttgagtgat atcgtctccg gcaaccccac
      841 ctgtatggaa tgtacgcatc gtcaactgag taccaggttc accgattgat tgagcggcga
      901 tcgtaccaac tgcttctccg acttcaacgt cagagccagt tgcaaggttg cgtccgtaac
      961 aatgtttgca gacaccatgt tttgtattac atgtgaagac agaacggatt gtcactgttt
     1021 cgacacctgc atcaacgatt tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa
     1081 tgatcaattc atttgtttca ggatgacgta cttctttacg ggtgtaacgt cccactaaac
     1141 gatcttctaa tgattcgatg atttcatttc cctcgcggat ggcttggatt tcaagacctc
     1201 ggtcagtacc gcaatcttct tcacggatga ttacatcttg ggccacgtca accagacgac
     1261 gagtcaagta acctgaatcg gcagtcttca aggctgtatc ggtcatccct ttacgagccc
     1321 cgtgagttga gataaacatt tccaagacgg atagtccttc acggaagttc gaaatgattg
     1381 gcaattccat gattcgacca ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg
     1441 taaagttgga aatgttacca cgagctccag aatcagacat catgaagatt ggatttttcg
     1501 cttccaaacc ttccatcagt ttttgttgga tgctgtcttt tgcagcattc caaacagcaa
     1561 tgacacgttc ataacgttcg tcatctgtga tcagaccacg acggaattgt tttgtaatag
     1621 tttcaacttg tttatgggca ttttcaatga tttcttgttt ttcattcagt acactgatgt
     1681 cagcgatccc tactgtgata ccggcatgtg tcgaatgttt gtaccctaag tctttcatac
     1741 ggtcaagcat cttagaagtt tctgttactt tgaatcgttt gaagacttct gcgatgatgt
     1801 ttcctaagtt tttcttcttg aacggtaata ctaattcttg ctctttgata tgtgcaggaa
     1861 tatcagtacc agcttcaaca aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg
     1921 ttggttcatt cagataaggg aattcaggag gcatgatctc gttgaagatg atcttaccaa
     1981 ctgttgtgat catcgtacgt tctttttgcc attcagtaaa tggtttttca cctaatgagt
     2041 tagggttcac accgatacgt gaatgtaaat gaacatatcc gttgcgccat gcgataacgg
     2101 cttcgttttg atctctgaag accattcctt cgccttcgcg tccttcttct tccattgtca
     2161 agtagtagtt acctagaacc atatcttgag aaggagtaac aactggttta ccatctttag
     2221 ggttcaagat gttttgagca gctaacatca acatacgtgc ttctgcttgt gcttcttcat
     2281 tcaatggaac gtggacagcc atttggtctc catcgaaatc ggcattataa gcttcacata
     2341 caagtggatg caaacggatt gcgcgtcctt gtactaaaac tggttcgaat gcttggatac
     2401 caagtcggtg aagcgtaggt gcccggttca gcaatactgg gtgttccttg attacgtctt
     2461 ctaaaacatc ccatacttcg tcttcttgac gttcgatttt acgtttcgca tttttgatgt
     2521 ttgaagctag ttcgcgttga acaagctcac gcatcacgaa tggtttgaat aattcaatgg
     2581 ccatttcttt tggaagtcca cattggtaca tcttcaagaa aggtccaaca acgataacgg
     2641 aacgacctga gtagtcgaca cgtttaccta gcaagttttg acggaaacga ccttgtttcc
     2701 ctttcaacat atgagaaaga gatttcaatg ggcggttacc tggtccagta actggacgtc
     2761 cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg cagcatacgt ttttcatttt
     2821 gaacgatgat tccaggtgca ttcaagtcta acaaacgttt caaacggtta ttacggttga
     2881 ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa
     2941 ccattgggcg taaatctggt ggaataacag ggataacgtc cataaccatc cagcttggtt
     3001 gatttcctga agcgcggaat gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt
     3061 tttgtccttg tgcagatttc aattcttctt tcaattcggc tgcttctgca tctagatcaa
     3121 cattatcaag taattgttta atcgcttctg cacccatagc agcttggaat tcttgtccat
     3181 attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg
     3241 tatttcctgg ttcgatgact acatatgaag caaaataaat gatttcttcc aaagcgcgtg
     3301 ggctcatgtc taatacaaga cccatacgtg aaggaatccc tttaaagtac cagatatgtg
     3361 aaacaggagc tgctaattca atatgtccca tgcgttcacg gcgaactttc gaacgagtaa
     3421 cttccactcc gcatcgatca caaacgattc ctttataacg gatacgtttg tattttccgc
     3481 aggcacattc ccagtctttt gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt
     3541 caggttttaa ggtacgatag ttaatcgtct caggtttttt tacttcaccg taagaccagc
     3601 ttctgatttt ttcgggagaa gccaaaccta tttgcatact ttcgaattta tttacatcga
     3661 tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc aagacggctg atcttcgacc
     3721 gccttgcagc tcatagaccg gattagtcta aattatcttc tgcggtttca aagttttgga
     3781 tgacatcgtc tttttcatct tccacttgtt cagcagcttt cttttctaat tctttggcag
     3841 tttgctgttc ggcaaatttt gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt
     3901 ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa gacacgcatg tccagaccta
     3961 gtgattgcaa ttctttcacc aatacgcgga atgattccgg tacacctggt tttggaattg
     4021 gttcaccctt gacgattgct tcgtatgttt tcacacgtcc gactacgtca tctgatttgt
     4081 atgtcaagat ttcttgtaaa gtataagccg caccgtaagc ttccagtgcc catacttcca
     4141 tttcaccaaa acgttgtcca ccaaattgag ctttacctcc caatggttgt tgtgtaacca
     4201 atgagtaagg tccgattgaa cgtgcatgca atttgtcatc gaccatgtga gcaagtttga
     4261 tcatgtacat cacaccgaca gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt
     4321 acagaacagt tttcgcatcg ctggccatac cggcttcacg aactgtttcc catacgtctt
     4381 catcacttgc accatcaaat actggcgttg ctacgtggat acctaactga cgtgcagcca
     4441 tacccaagtg taattccaat acttgcccga tgttcatacg tgaaggcacc cctaaaggat
     4501 tcagcatgat atcgattggt gttccatcag gtaggaatgg catatcttct tccggcataa
     4561 tacgggatac aaccccttta tttccgtgac gaccggccat tttatcccct tcatggattt
     4621 tacgtttttg aacgatataa acacgaacta acatgtttac gcctggtgac aattcatctc
     4681 cagcttcacg agtaaagatt ttcacatcgt gaacgatacc gccgccgcca tgtggtacac
     4741 gtaatgatgt atcgcggact tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt
     4801 cttctgcaga taattctgtt accccttttg gcgtgacttt ccctacaagc aaatcgccat
     4861 cttggacttc tgcaccaata cggatgatac ccatttcgtc taaatctttt aatgcgtctt
     4921 ccccgacatt agggatttcg cgtgtgattt cttcaggtcc taattttgta tcgcgggctt
     4981 ctgattcata ttcttcaata tggatagaag tgtatacgtc atcttttacc aaacgacggc
     5041 tcatgatgat cgcatcttca tagttgtacc cttcccaagt catgaaacca actaaaacgt
     5101 tttggcctag agccatttcg ccttgttcca tagatggtcc gtctgccaga gtatcccctt
     5161 tttcgacttt ctcacccaaa tgaacgattg gacgttggtt gtagcttgtt ccggagtttg
     5221 aacgacggaa ttttgttaca tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga
     5281 tttcagaagc atcaacgtat tcaacgacac cgtcatgttt acataacaaa gcagctcctg
     5341 agtcatgcgc tgatttgtat tccataccag taccaaccca tggtgcttga ggattgatca
     5401 acggcactgc ttgacgctgc atgttggcac ccatcaaggc acggttggag tcatcgtttt
     5461 ccaagaaagg aatacatgct gtcgcaacgg caactacttg tttaggtgat acgtccatgt
     5521 agtctacttt atcaatagat acttctaagt tttcactttg agcacgagcc ataacaacgt
     5581 cttgagcaaa tgttccatct tcattcaatg ggctgtttgc ttgggctacg atgtaatggt
     5641 cttcgatatc agctgtcaag tagtcgattt ggtcagtaac acgacctgtt tcacggtcta
     5701 cacgacgata aggtgtttcg ataaatccga atttatttac tttcgcataa ctggacaaac
     5761 tattgatcaa cccgatgttt gggccttcag gagtttcgat cggacacata cggccatagt
     5821 gggagtagtg aacgtcacga acttcataac cggcgcggtc acgagtcaat ccgccaggtc
     5881 ctaaggctga aagacgacgt ttgtgtgtca attcgccaag agggtttgtt tgatccatga
     5941 actgtgacaa ctgagaagaa ccaaagaatt ctttgatact tgccacaact ggacggatat
     6001 tgatcaattg ttgaggtgtc aatgtttcag tatcttggat cgacattctt tcacgaacca
     6061 cacgttccat acgtgctagc ccgatacgga attggttttg taaaagttca ccgactgaac
     6121 ggatacgacg atttcctaag tggtcgatat catcgacatt gccgatacct tccatcaagt
     6181 tcaagaaata gctcattgaa gcaatgatgt cggctggacg aaccgttttt acagctgctt
     6241 ctggatagcc gtttccgatc acgtttactt cacgttcagg gtctcttggt gaaaatactt
     6301 tgatgacttg gacagtcatt ggatctgtga ccacaccgtc ttctgatgga tagtaagtca
     6361 ctgaatttag gccattatca atgaaaggag ctagtgtttc catgacttgg tgagtcagaa
     6421 ctgttccttt ttcaacgatg atctcgcctg tttctggatc aactaatgtt tcagctaatg
     6481 tcagattcaa tagacgtgtt ttcaaatcta attttttgtt tactttgtaa cgaccaacgt
     6541 ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt
     6601 ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg
     6661 ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc
     6721 tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa tcctaacgca cgaactaata
     6781 cggttaaagg aatcttacgt gtacggtcga tacgtacata agagatgtct tttgcatcag
     6841 tttccatttc tagccaagct ccacggttag ggataacagt agaaccgaat ccttcttttc
     6901 cgtttttgtc gacttttcca tggaaataga cacctggtga acggactaac tgagaaacga
     6961 ttactcgctc tgccccgttg atgatgaatg ttccttgttc tgtcattaat gggaaatcac
     7021 cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta
     7081 catgtaatgg tgcagagtag tttgcatcat gcgcacgagc ttctgctact gtatactttg
     7141 gttctttcaa ttcataatct acaaattcca aagatagatt gccattgaag tcatcgattg
     7201 gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa gaaccattgg taagagtctg
     7261 tttgaatttc gatcaaattt ggtaattcca acacttcact gattcttgcg aaacttctac
     7321 gttcgcgatg ttttccgtat tttactacgt gtccagccaa gctcttcacc cctctaaaat
     7381 gtcattcaaa aagactaccc gatcaattgt tacaataata tttcaaatat tatttttagg
     7441 taacaacaga cattttttgc ggcttttagg caaaaaaaaa ccaaaaatag aacattctct
     7501 tgaaacctta cttcgtgagc ttctactttt gtttccttat attaaagccg aaacggacaa
     7561 tatttcgttt ctgccttttc ttcatcagat agtttttgca acttcatata ctacacttat
     7621 tttctataaa agtcaacact tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat
     7681 gttttcaatt ttatgttttc aattatctga caattcttaa ttgacagacc tttttatctc
     7741 ggttatactt tagaaaattc attttgtgga ggaacttcta tgcgaaccaa catcaaccaa
     7801 atcgcaccaa cacaactgaa taacttttac tttagctact ttagataggt ttgtattcat
     7861 catgatggat acaaacgtta aacgacagtt tgtatctatg atggctaaag aactttgtat
     7921 tgcagcaaaa agccacgtag acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa
     7981 aagtatcaag cattctactt tttcaattta ttttccgact tttcaatcca tttggacatt
     8041 ttgtctgaat ggattttttg tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata
     8101 taatcatcgg attaggcgct gccctgctac tagctttagg aggttgcgca caaaaagaca
     8161 attccgcaga ggcatcagat ggacaaaaaa caattggcgt attgcaactt gtacaacata
     8221 actcgttaga tgctgcctat aaaggattca aagaaggatt agcagaagaa gggtataaag
     8281 aaggcgataa tctaacaatc gattatcaaa atgcccaaaa caatcaagac aacctgaaaa
     8341 gtatgagtga gaaactcgtc aaagaagatc ctgatttgct acttggaatc gccactccag
     8401 cagcacaatc tttattaaat gaaacaacca ctatcccgat gactgtcaca gcggtcacag
     8461 acttggaaca agcccattta gtcacatcca atgaaaaacc tggcggcaat gtgactggca
     8521 cgacagatat ggtgccaatc gaaaaacaaa tcggcttact gttaaatatc gtgccagacg
     8581 ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa ctctaagatc caagctgatt
     8641 tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca
     8701 caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga tgtagatgga atctatattc
     8761 ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa
     8821 aaacacctgt cgttgctggc gcagttgatc aagtcaaaga cggcggtcta gcaacagtag
     8881 gaatcgacta cgaaagcttg ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg
     8941 gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga tttggaactg tatgtcaata
     9001 aagacatggc caaagcatta ggcatcaatc cagacagcat caaagaagct gaataaaaca
     9061 caggaggatt ctcatgacaa atttgctctc tcttttctta tcatctgcat cgcaaggggt
     9121 cttatgggcc ctacttgcga ttggtgtata cgtcactttc cgtatcttgg acattgccga
     9181 cttgactgct gaaggaagct ttcctttagg tgcagggatc gcggcagtaa gtattacaaa
     9241 tggctatcat ccattgacag cttgtcttct aggcttttta gggggtgcgg cagcagggct
     9301 tgtttccgga ctgcttcaca caaaattgaa aattcccgcc cttcttgcag ggatcatcac
     9361 catgactggt ttatattctg tcacatcccg tgtgatgggt gcgccgaaca tctctttatt
     9421 gggtcagcca acgatctttc attgggtcga gtcattcggc ataacaaaag acaacgctgt
     9481 actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt ttacttgttt tgttcttacg
     9541 tacagaaaca ggacttgcta ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa
     9601 cgggatcaac acagataata tgaagatcat cggttatatg atttctaatg gatgcattgc
     9661 tctttctggc tccttacttg cgcaaaacaa cggctttgct gatttaaatt ctggtattgg
     9721 aacgatcgta attggacttg cttctattat catcgcagaa gttttgttac gtaaccagcc
     9781 actatttctc cgtctcttga ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc
     9841 gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct
     9901 agtgatctgt ctttctttcc cgcagatcca gcaaaaattc catttacctc aaaaaaagaa
     9961 aggcggtagt gtcaaatgaa tgacgtctta acattggaaa atctccatca tacctttgaa
    10021 aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta atctttcctt agatcccggc
    10081 gacttcgtga cgatcatcgg tggaaatggg gctggtaaat ctactttgct gaacagcatc
    10141 gccggtacat tttctgtcga tcagggtcaa attttattga atggaaaaaa tatcacgaaa
    10201 aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat tccaagatcc taaactaggg
    10261 acagccgttc gtttaacggt agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa
    10321 cgcggcttct tccgtggtgt caaaccacaa gaccgaacat ttttcaaaga ccagctggtt
    10381 cggctaaatt tggggttgga aaatcgttta tcctcagaaa tcggtttgct gtctggagga
    10441 caacgtcaag ctattacgtt attgatggcc acattacagc gtccagaact gatcttatta
    10501 gatgaacaca ctgcagcgct tgaccctgct acttccaaaa cggtgatgga attgacagaa
    10561 aagctgatca ctgagcagaa tctcactgct tttatggtca ctcatgacat ggaagacgcg
    10621 atccgctatg ggagtcgact gatcatgctg catcaaggaa ggatcgtcgt agatatccag
    10681 caagaggaaa aacagcagct cacagtgccc caattattag cgttgttcaa acaaaacagc
    10741 ggcacggcat taaaagacga tcaagtactt ttatcttcat aatcagaaag aggatgggac
    10801 aaaaaatgaa cgcccaccct cttctttttt ctctttcctt ctttcgtttt tgctttatgt
    10861 atcaaccgtt gtttcttcga aaactatttt gttctctcct tcttcctgat agaatggatg
    10921 ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca
    10981 ccagattttc tttccggaga aaagttggca cagcaactgg aagtttcccg gacaagtatt
    11041 tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg
    11101 tatcgttacc tgccctctga cgtactagat gcaaaagaaa tccagcaaga gctacggtcg
    11161 tttattcctg atttgaaagt acaaacgatg ttgacttctg aatctacgat gaaagatgcc
    11221 aaactggcag cagctcaagg agattctgtt tcgaagttat ttgttgctga tacacaagaa
    11281 caagctcgtg gacgttttgg acggcctttt ttcgctgaac ctggacgagg aatctacatg
    11341 agtctcttgc tccatccaga caaacggttt gatgaattgc ctcaatatac gatcatcgca
    11401 gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc gttctgcttc gatcaaatgg
    11461 gtcaatgata tctacatgga tggaaaaaaa gtctgtggga ttttatctga agcagtcagt
    11521 gatatggaaa gcggcaggat cagtcatatc atcattggga tcgggcttaa tttttctgtt
    11581 cctcaaaatc attttccagc tgatctccaa gaaaaagcca tctctgtctt tcccgacgga
    11641 caagctagtg tcacaaggaa tcagctaatc agcaaaatct ggcaagaatt gttcacttat
    11701 ctttctcacc cagaaaaagc gttggacttc tataaagaaa aatcattcgt attgggaaaa
    11761 accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat
    11821 catggcgaac tgatcgtcca aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc
    11881 agtctcacat ctatccgtac gcaataatta tcagatttca caaaaggaca cctgatctca
    11941 tacagtgtcc ttttttctga attctcttca aaagactctc cttattctat cagctgacag
    12001 tttctctatc ctctagaaaa aggtattcta aaaatatcta taaagcatgg tacactttct
    12061 ttaaatgaat caatgcaaaa aacaagagag tcatcagttt acacgatcaa aagagatagc
    12121 aggccgcttc ctttttcaat cttatagata gaccatgggt aacgataaaa acaaaaaaga
    12181 acttgcgact tattttgccg taagttcttt tttagctagt gccttaatct cttaatccca
    12241 acaaacgtct cgcatagatc acctgaccag agtgcatagc agcatcatca ataatagaaa
    12301 cgagacgcat tcctcgtgtg actggtggtg tccagttctc gtcaattact tcatctagct
    12361 cggaaggttc cagccttctt aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc
    12421 ctagcagatc ttctatttga ttagcttgga tctgctttgc ttgttccaga ctatgcttcc
    12481 aatcttgttc gttatctgct atcgtcatcg gaaaccggct ttcccagtct tgactatgcc
    12541 aaattggaga agttcctgct aaatcagcaa tctggaaatc taattctcga gcggtatgcc
    12601 aagccagcca agtcattgat ttgatctgcg gggctctatc ttccactgga aaacgattgg
    12661 cctcctcaat agagacgcct tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac
    12721 tctcgcatag catatctaaa taatccattt attttcctcc tttttattta tcctacttac
    12781 tttttcctaa ttctaggata cctacttata tgttactaca cacccttatt ttttacactt
    12841 taacagtcac catcatcaat agtttggaga ttgaaagaaa aagatagtgt cgaactatca
    12901 attgaacaag aagcaacaat atgaacgttt cattaatatt ctttaaataa acaatgattt
    12961 ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa taggaaaatg tttgaaagga
    13021 aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt cctagttgct ttgttgttca
    13081 atatcttttc gtatggtgtg gccgtttcag cggaaacata cattcctaaa gaccctggga
    13141 atatttataa tgatctaact ggtaactatg gcttgttagg aattgctagt caattccacg
    13201 tatttgctaa aaataagaca actgtgaatg cacatacaga cggaaatata gcaacacatg
    13261 aacttgatgc aaaaaataat tttggtacag agcttcatga aggtttattg aaccaagaaa
    13321 taaattatat acaaaaaact gattctttcg ctaattcttc aggcatacct acaggtagca
    13381 caagaatgaa taaatttgtc gttggcagta acgtggatgt agcacttcaa gatggtcatc
    13441 ctacagtcaa tggatccagg cttgatcact taactccaga tgatttttat aaagatcgtg
    13501 ctgggcatga atatattgat ttcgagcagg aatttgctaa attgaatgta gcagcaaatg
    13561 atcttgcaac gatcactcca gctaaaacat atactgctgc agattttcca gacatgaaca
    13621 atcggacaat cgatttaact gatttatcag ataccgggtt tttattggta aatatcgatg
    13681 cagaagtgtt aacgatgaat acgccacttc aaatcattaa tccaaatgat caagtagttg
    13741 tatttaatgt catcaacagt tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg
    13801 atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa tatctcttgg aattttggaa
    13861 atgagatgac tgatttgaca atcagtgctc cttttcaagg aacaatcctt gcgcctaatg
    13921 ctactatccg cattactcaa aaccaagacg gaacgattat aggaacaaat gtgattttag
    13981 atgcagctac caatcgttgg gatcctaacg aaatatttat cagcgacaat ggtacagata
    14041 cgacagacac aagcgacagt acagatacat ctaccacaag tgatagctca gattcatcaa
    14101 cttcaagcga cagtacagat actacctcta ccacaagtga tagcacggat acatcagctt
    14161 caagcgacag tacagatact acctctacca caagtgatag cacggacaca tcagcttcaa
    14221 gtgacagtac agatactacc tctaccacaa gtgatagcac ggacacgtca gcttcaagcg
    14281 acagtacgga tacatctacc acaacaagtg atagcacgga cacgtcagct tcaagtgaca
    14341 gtacagatac tacctctacc acaagtgata gctcagattc atctaccact acaagtgata
    14401 gcacggacac gtcagcttca agtgacagta cagatacatc ctctaccaca agtgatagct
    14461 cagattcatc aacttcaagc gatagtacag acacgtcgtc taccacaagc gatacacgtc
    14521 atgcaagtgt ctccttctca aaaacaagtt cggataacag gacgaacttt aacttaacta
    14581 ataaaacaag caatgacaat ggaaacaaat caaaaagagc acttcctaaa actggaagtc
    14641 aatcgaataa ttggataaca ctcgcaggcg ttatattact tgtaatcggt ttaagaatca
    14701 cttttatgag ttataagaaa tcgaaaaggt aaaaaaatat tcaagataaa aaagagcaac
    14761 gtcgctaacg gacgttgctc tttatttttg ctttttatca taaatacaag aactacaatt
    14821 caatttgtca aaaaacacgt agatttcctt ctgagaaatt tatttacgta tcgaaccttt
    14881 ccctattcag tctttgcgat ccaaaatcag ttgggtaagg gaaaagagat tctcttttgt
    14941 tcgaagagag gtggcaggta atttctttat ttccttcaaa gccttttctg tgtaagaggc
    15001 cgctaacttc ctagcttctt ccacgcctcc tgtatgttca acgattttgc gtacggcgtc
    15061 gacatcttct tctgtcattg ccgctttctt ctctaaataa ggtagtagct ttggtttgtt
    15121 tttttgcaaa gagtagatca acggtaaaga atagacccct tgacgcatgt cttctagtac
    15181 aggtttgccg atttcctggc tctcttttgt ataatctaaa atatcatcga taatctgaaa
    15241 ggccatcccg atagaaagtc cgatatctcc tgcttttttt gcgaagcgct cgcttgttcc
    15301 actttcaaaa gcgccaacag aacaacttaa ttggaacagt tcagctgtct tacctgaaat
    15361 attttccaaa tattctttta cagaaagttc ataattatag cgatcatcca tctgccctaa
    15421 ttcgccattc aatacttttt ccatactgcg agaattcaat tggagacttt tcaaggaagt
    15481 ggagtaatca gacaatagtt taaaacagca gacaaataaa tagtctcctg catagacaga
    15541 aacgacatta ccaaactgac tacggatatt aggctgacca cgtcgcagat ccgcttcatc
    15601 aacgatatca tcatggatca gtgtggccgt atggagcatt tcgatagaag ctgccaaagc
    15661 gatggctttg tctcgatctt gctctggtcc aaattgtgag aacaatagct gataggcagg
    15721 acgcaacagc tttccgccgg catggatcgt ttctttgatg gcttgttcga caggtttgtt
    15781 tttcaggcgg acagtttctt ccatccgttt tagtgtttct gtcaattctt tacttaaagc
    15841 aggatagttt tcccacattg gatgtagctt cataaaaaaa ctccttgtgt gacgattatt
    15901 ttttggtaaa cattgggctc catttttgtt tggttccctg ctcataatct ttttggaatc
    15961 gttgaagtgt ctgcacatgt cgtaaaagat aattcgctgc gatcccgatt gcaatccctg
    16021 ataaaatccc taaaactgaa aggactggca agtacaacat gactgtccat gattgtgcaa
    16081 tgaaagaagc catcaaaagc tgtccgacat tatgcatgaa accgccggcc gcactaattc
    16141 cgatgatact gacacgtttt ggtcctaatt gcttgaccaa taacattcca aaataactaa
    16201 gcaaagcacc actcatacta taaaggaatg tggaaatcgt tccgcctagc aaagtagtca
    16261 ggaatagtcg tagccaaagt agtaagaagc tgtcttgttt tttcatggta aaaatggcaa
    16321 cgatcgtgat tagattggct aagcctagtt ttgcaccagg cgcaaaagaa aaaggataag
    16381 ggatcatatt ttccaataaa ccaatgatca ctccttgtgc aacaagtagc gaaatataaa
    16441 tcattttttg taatttactc atggtgtgtg aaggtctaat aaatcagact gtcttcgtca
    16501 cctccatctg ttgaacggac ttcgatcact aatttatgtg gcaaacagac gatggtttcg
    16561 ccatttttcg ttgcccagcc tcgacgtacg cagatctggt catcgcaatc tgcttctttt
    16621 atccgtactc gatcgccatc tatctcgatc aagttataat ctccgtgttc atctttgtaa
    16681 gtatatgtgt tgctctcttc tccagccatc aaagggaatg ttttgatttc tgtgccatcc
    16741 acacgcagta ccgcttcttt ttctgctgtt gcggtgtttt gctgaagacc aaaaatcatg
    16801 actggtaaaa aagaaagaac agtcaaaagc aaaataataa aaacatccca aggtttgagc
    16861 agactttttt cagcaaattc tttaaggctc atttttgttc tcctttttcc agattaactc
    16921 acccaatttt agcataaaaa caagaggttg tgacacctgt gtcttgcttt gagtaataag
    16981 aacgaagaat agaaaacagc tttccatgtt ttctaattct ttgggcttat taccgagaag
    17041 cagcaggtgg aacaccgttt ataacaagag gttgtgacag aagtgggcaa cttcaagaaa
    17101 taagaaggaa tttacgaaaa ttacttttca agtttttgtg aaatttcggc ttatttttcg
    17161 aagaagctac ttctggaaca ccgtttataa caagaggttg taacagaggt tgtgacacct
    17221 gtgtcttgct catggaggct tatctcgggc atcttattcc tttaatttta cataaaaagg
    17281 aggctgtgac ggtttgtctc agcccctttc tattgaaatc ttgcattcaa ataacgttct
    17341 ttatctattt atttcttgcc gtactgggaa cttggtctac cgtaccacca attacctagt
    17401 gtacgttgta gccatggaat tacccagcga tccaacccaa tcgcacgtcc ggaaccgttc
    17461 atcaatgcga aagcaacaaa aatgaaccaa atgtttaccc agtaaaacat gcctgataag
    17521 cagaacgtga tcaccaatac aatcgttgtc gcactgcata gccatgtgaa taatccagca
    17581 atcaatgcta atgccaaggc aatttcaaag aataccatga atttttggaa gaacattgct
    17641 acatcattgt ttggcatgat cacttgcatg atctttgtga accagtctgg tgacttctca
    17701 atcacagcca ttggttcttc accgtatgca taactcaaac caaaatgtgc agcttgagtc
    17761 gctgcttctc cggcacttgc ccctgctccg ctggcagctg tggttgcatc accagcagca
    17821 gaagcaccac tggcaacatc tgttacattg tattcccatg tccaagggaa cacactgttt
    17881 ccagtgaacc atgatccttc accaaaccaa gtacttggtt tgaacacttc accattccca
    17941 atgatttttt tggaagcttc aacgaaccaa actaaaccat agaaaatacg aagtggcaca
    18001 ctccataaga cattgccgta acgagaagaa tgtcctcgag tcacgttgcg atcatctttg
    18061 atatggaaaa actcatgcat taaatattgg aacatataat aaccagaacg aatatcaaag
    18121 aagtatttca aattcacgat atgtttcatt acgatagcta aaaatccgct taaatggatc
    18181 ttatcaaata ggttagctac cccccatttg gcaccaatag aaaccataaa tccttgatag
    18241 tttcctttaa atttatgctt ttctccgcct ttgatcgaag ccacgatatt cgctgcggct
    18301 gtgtgtcctg tttgttcagc agcttgaacg atctgcggtg tcggtgttcc tgggaattct
    18361 tcataaaaga ctaaatcacc aatgatatag atgtttttat cttcgtagcc ttttgcctgc
    18421 atatattcat tggcgatcaa ccgctgtccg cgtgctgatt ctaatccaaa gtcagcagcg
    18481 tcagaagttc ctttgacacc agctgtccaa atcagtgtgt gagtcggaat gttgcgtccg
    18541 tctttcaatt tgatatgatc agcagccact tctacgattg gtgcattcaa taataattcc
    18601 acgttgtgct tacgcaaata gcgttcagct tttgcagcat catttcgatc caacatattc
    18661 aagatcgtcg gcattgcctc tacgaccatc agtgtgattt cgtttggatc taatttataa
    18721 tctttggcta ggcgattttt ccagtcgatc agttccccaa tcatttcaat accggtaaat
    18781 ccagaaccac agacaacgaa agtcagcatg gctttacgca gttcatcatc tggttccaat
    18841 gcagcttttg ctactgtctt ctcaatatgt tcccgaatcc ttacagaatc ttcaaatgac
    18901 caaagggtga atccgtgtgc tttgacacca ggtgtaccaa agtcattcgg ctctccgccc
    18961 attccaagga tcagctgatc aaaaggatag gaacctaatt tggtcttgac gatttttcgt
    19021 tctttatcga taccgattac agtgtctgtg acaagttgta cattcttttg acgagcaaac
    19081 aaatgctgca gatcgtattg gattgcttct ggttctacac gtccgccagc tacttcatgc
    19141 aactcggtca tcatagtatg gtacgaatga cgatcgatca gagtaattgc tacatcttct
    19201 tttttcagtt tctttgccaa gtattttgta gctgaaacac cggcatatcc tgctcctaca
    19261 acgacaatat tttgctttgc cattgatcat ccgctcctta tagactatct atataatgaa
    19321 tatatgataa ttcttttagt ttgatactta ttacacaatc tccatatatt atatagatgt
    19381 tctgttcttt tgtctaactc cttttggatt tttttcaaat tttaaaataa agcgtttttt
    19441 cacgaaattt gctgaaagaa aacgtttgaa atattacatg ggttatttat caaaaatagt
    19501 tgacttatag gctaaaaaga ttagaatgta taatgaatca caaagaaaaa ttgtggacta
    19561 gaaatggaat ggtggggtat taaaatgaag aaaaaacgtt ttgtaactgg atttgccgta
    19621 ttagcatttt cagctctagt gttaggcgct tgcggcgcag atgacaacgg aagctcaaac
    19681 tcttcttctg aatcatctac agcacaatct tcgactgcaa aaagcacaac agaatcatct
    19741 gcaaaagttg tagctggcgg agatttgcaa gacggcacat ataaattaga agaaaaaaat
    19801 tattcaaatg gttaccgtgc agtctttgaa atggttgtaa aagacggcaa aatcactgaa
    19861 tcaaaatatg ataacgtcaa tgaaaacggc gaatcaaaaa caaaagatgc agaatacaac
    19921 aaaaacatgg aagcaaaatc tggcacaaac ccagaaaaat acattcctga gttgaatgac
    19981 caattcttaa aagcacaaag cgcaagcggt gtggaagtcg taactggagc tactcactca
    20041 tctgaaagct tccaaaacta cgcacaacaa ttgatccaag ctgctcaagc tggtaacaca
    20101 gatacaatcg aaatcgacaa tggtgctgat ttgaaagacg gcacttacaa gcttgaagaa
    20161 aagaactact ctaacggcta ccgtgtgcaa ttcgaaatga cagttgctgg cggcaaagta
    20221 actgaatcaa actttgatta tatcgacaaa gacggtaaat caaaacaaga tgatacagaa
    20281 tataatgaaa atatgaaagc aaaaagcggt acagaaccaa aaacttacat ccctactctg
    20341 aacgacgaat tcgtcaaagc aatgggcgaa gaagatggct cacctgctaa tgtagaagtt
    20401 gttacaggtg ctacacacag ctcacattca ttcatcatgt acgcacaaca attagtgaat
    20461 gcagctgaaa aaggcgacac tcaaacaatc gaagtagata atatcgtaac agaaaaataa
    20521 tcacataatc atactaataa aagttggagg gtgggacgat agccagatta ggcagtcgtc
    20581 tcacctttca aaatgaagag aggaaaaaga tgcggaaaaa actactattt tccttaatca
    20641 cactgggcat ctcttttatg gtgctaactg gctgcagcaa ctcagcaaat aaaacaacca
    20701 ctgaaagttc agcagctacc aagatattga aagatccata ttctgatgaa cagttcttgt
    20761 tagggactta cgttcgtatc cgtgtgtatg atgaaggcaa agaatcagca ttgaaacctg
    20821 ccttcgatcg agtaaaagaa cttggtgata aaatcacgat caatcaaaaa ggttctgaga
    20881 tcgatgaagt gaatgagcaa gctgggatca aaccagtaaa agtttctgat gacgtctaca
    20941 cgttagtcaa gcgggcctac gaatatagcc aagattctca aggcggattc gatatggcga
    21001 ttggtgcaat cacacaatta tggcgaatcg gttttgatga cgccagaaaa ccttcccaag
    21061 aagaaatcga tcaggcattg aaattagtgg attatcataa gatcgaacta aatgacaaag
    21121 aaaaaactgt ttatctaaaa gaaaaaggaa tgatcatcga tttaggtgca atcgccaaag
    21181 gttacattac ggacgaagta gtgaaagtct tgaaaaaaca aggagtgacc actgccatcg
    21241 ttgacttggg cggtaatgtc tatgtcttag gccacagccc aagaggcgaa aaccaagact
    21301 ggacagtcgg catccaagat ccgaacatgg ctcgcggatc tgttttaggt tctattaaag
    21361 aacggaacaa aacattagtt acctctggaa tttacgagcg ttatttagaa gtagatggca
    21421 aaaaatacca tcatttgttt gatccaaaaa cgggttatcc gttcgacaat gatatcgcaa
    21481 gtgtcacgat cataacagat aaatcaattg atggtgacgg gttgtctaca gcagtctttt
    21541 caatgggtgt gaaaaaagga ctggaatacg tcgaaagcga attgaataac gggacggaag
    21601 cgatcttcgt gacaaaagac gataaagtat atgtgaccga tccaatcaaa gacaccttca
    21661 aattatcgga ggactcccat tacacgatgg gtgaccgttc tgaactaaaa taaggagtac
    21721 gaaccatgac actaaaaatc tttttagaag tagttgaaat acgaacaaag gtggctagtg
    21781 tatttccttt tgcggtaggg gtattattct ctatcgcttt ttttcatgag atacattggc
    21841 taaacacttt tttatttttt attggaatgc tagtttttga tttagcgact accgctatca
    21901 ataattacat ggattatgaa aaggctcatt ctgaagtata taagtatgaa gaaaatgtca
    21961 ttggccgcag aaatctatct cctgtattga ttcgaaacat gattttagct atgattgcat
    22021 ttgtttttgt tatcggcgtt attctaacga tcaatacggg ttggctttta cttttgatgg
    22081 gatttatctg ttgttttatt ggagtattct atacattcgg accgattcct ttatctcgaa
    22141 tgcctttagg ggaaattttc agtggggtga ctatgggcct tggaattttt gccatggtcg
    22201 tttatgtcaa tacattcggt tcagatgtct atgatctgac agtcaacttt tcatctggac
    22261 aatttgattt ggtaggaaat ctttgggcta tagcagcaat cgtattagct tccttaccct
    22321 tggtgtttac gattgccaat atcatgttgg ctaataattt gcgggatctt gaccgtgata
    22381 ttgaaaatca tcgctataca ctagtttttt atatcggacg tcctgcggga acagtcttat
    22441 tccagctatt gatgtatgct tgctatttcg tgattttaat ggggttgttg agcggtatct
    22501 atgaatggcc gatcttggtc accttcttga ccttgccaat tgtgaagaag catttggatc
    22561 aattcaagca aacgttaccc cagcctatca gtttcagcta ttccatcaaa aatatggtct
    22621 tattcaatgc cagttatgta ttggggctat tactaacaat cattatgcaa gccatttaac
    22681 agcatgaaga ggttgtgaaa aaactgccct gcatcaagta ataagaacag ccaaataaaa
    22741 atagctcctt atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca
    22801 ccgtttatca ggaggttgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg
    22861 aaaatggctt tccacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
    22921 caccgtttat cagaggttgt aaaatcagtg ttttgtcctg agtattagag aaacaaatgg
    22981 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa
    23041 cactatttgt tcggaaaaag tgactaacta tgacacaatt tttttcacag tccctttttt
    23101 cacttgattt gattcgtacc agcgaacttg acgagaactt tcttggtttg gtaactatat
    23161 ggtattcttt taacatcctc gtttgcttct tctaatttat ttatacaaag tcacactttt
    23221 ttaaacatct gttcttcgat acatttctat ttcttttaaa catcgatacc aatattttga
    23281 acttaagagt gcataccaaa tggattcatt tactcttcgt cagttacgaa gataatcaaa
    23341 atctccaact tccctactta tttcgttttg agaattatcg aagcaatctt acataaataa
    23401 ctaaaaaaca aaaaaccaga aaatcagtat actgattttc tggttttaca tggtttgttc
    23461 aataaaaagc tgttggaact gttaatatta aattatcgca tcgttacgaa ttcttccgct
    23521 cctgttggat ggatggctac tgtattgtca aagtcttctt tagttgcacc catcttgata
    23581 gccacggcaa aaccttgcag catctcatcg acaccaacac caatagcatg taagccgacg
    23641 attctttctt cttttccgac acagatcaat ttcatttcgc atttttggcg ataatcattt
    23701 aatgcaaagt acattggggt aaagcgtgaa cgataaactt tcaattcatt ttcgccatat
    23761 ttttccaatg cttcttcttc tgtcaaacca attgttgcaa ttggtggatg cgtaaagacg
    23821 actgtcggaa caagtttata gtcaagataa gaatctttct ttccattaaa taaacgttct
    23881 gataaacgtc taccagctgc gattgcaaca ggagtcagat ctaatttacc gatcacatca
    23941 ccaacagcaa aaatatgttc atccgttgta ttttggtatt tatctacttt gataaaccct
    24001 tttttgtcca attccacatt tgtattttcc agacctaatt tttcggtatt cggtacacgt
    24061 ccaccggcaa aaagtacaca atcaccagtt aaggtttcac cattttcaaa tagcaccgta
    24121 tattcgtcat tgtttttagt aatttcttta gcaatatgat gtgcgtaagt ttttattcca
    24181 gcttcttggt acatttggat caaattgtcc gaaagcattt tatcaaacgt tctaagtggt
    24241 cgttcttttc gatatgccca catgacttca ctacctaaac cacgaaatac acctgaaagt
    24301 tcagccgcaa tgtatccagc acctaaaacg attgctctct taggtaactc tgttaattca
    24361 aaaaatccat ttgaatcaat tgcgtattct ccaccaggaa tggtcatttt acttgcacgg
    24421 cctccagtag cgatcaagat tttaggagca cgataggttt caccgtttac ttcgatcgtt
    24481 tgactatcga taaaagtagc gtaacctttt atagcctcga catgattgct atctaatcct
    24541 ctttgataag caccgtgtag aaaatcaata tatttttctc gtctttctac tagaccggcg
    24601 aaatcaacat tttttaaatc tgcttggata ccgtaacttg aggcatctcg ttcaattgtt
    24661 tccaaaatcg tacttgcttg ccacataact tttttaggta cacaaccgac gttgacacat
    24721 gttccgccca attcattcgc ttcaatcagc aaaacttttg cgccatgcat ccctgctctg
    24781 tttgctgaag caataccgcc agagccgcca ccaattacga tgtaatcata tatttgcatt
    24841 ttgttcctcc ttattgaaga gatcaatcaa aacctttatt taacgatcct ttttctacag
    24901 aaaacaagtg taacactttt cttgaatttt gtatgcgaag atgcttaaaa cagggatata
    24961 tcagcttttt ccttatttac gttttcggat catatctcca ggtttcaccg gctgtgcgac
    25021 tggcatagtt aaaatcatca ttggatttgg tgctcggtca atggattcgc cttcttcatt
    25081 atgcataact tcaacggttt gactgaaatg actgaatcct ggaccataaa attcgatttc
    25141 atctcctaca gaaaacaagt ttctctgacg tatcgtagca atctgtgttg cctcatcata
    25201 agataatact tcaccgacaa atttatatac tgggatttta cgacgttcac cgaataattg
    25261 ttcgttttca gttggtgtat tgtagtaaaa acctgtagct aattctcgtt gtgctacttt
    25321 ccataactcg tcgatccatt cttgcttgca gacatagttt tcaggatctt ccatataact
    25381 gtctacagcc gctttataga catttgacac cgtagataca taatggatcg atttcattct
    25441 tccttcgatc ttgaagctgt ctacgccgtt ttcaatcaat tctggaatat gttcgatcat
    25501 tgacatatcc actgcactca tcgagaattc ttcttccact tcgcctgtat cagttaaact
    25561 gtttcgttct tgaccaaaag gcatatcgaa caatccatac ttccagcgac aagattgaga
    25621 acatccgcca cggtttgcat cccgcatcga catatggttg gacaatgtgc atcgaccaga
    25681 ataagagata cacatggcac catggataaa tgcttcgatt tcggtatccg tatttttacg
    25741 aatctctgcc acttcctcca ttgaaacttc acgagctaaa acgacacgtt ccaacccttc
    25801 ttctttccag aattccaatg tctcaaaatt agttgctgaa gcttgagtgg ataaatggat
    25861 cggcagacct ggggcttctg tcgcacaaat ttcgatcaaa gctggatcag aaacaatcac
    25921 tgcagaaata ccgacatcac gcaaactgcg gaaaaattcc cctgctccat cttcatttcc
    25981 ttcatgggtg accatattag cagcaacgta taccttcgca tcatgttctt ttgcaaaagc
    26041 aactccttca gccatttctt cataagtaaa attacctgca cggcttctta aaccatatgc
    26101 attcccgcca atatatactg catctgctcc ataatggatc gcggttttta acttttccag
    26161 tgttcctgct ggcgctagga tttctggtcg tttcaatttt ctttttactg tcatttcttt
    26221 catcatactc cttatttgat agcagaagga tcaatataaa agaatcctgt atctaattca
    26281 cggttttcag gatgcaattt actgatttcg ctagtcattt tttccgcttt ttcttgatcc
    26341 cagctacctt ctaaaatttg tttttttgct tcggcgaata gtcgagtgat gtttacgaat
    26401 ttttcgcctg gagtaaataa tccgtccaat ttccatgtac agtaatgatg ctgataaagt
    26461 tcattcaatt cattcgagag attcagatca ttgctggcaa aaatatgtgt gccatgacta
    26521 tcttcataaa tagaataatg tgtctcttct tttttaggtt ccgagataaa aagacctctt
    26581 tcacgatctt tttgttcgtc ttgtttcgta tagttatagt aattctgtag cagtggacgt
    26641 ttggattgat ggatgcatgt agcaccatac acaagggttt ctacaggaat atcgagtttt
    26701 tcttccatcg ccttcatctc ttcaaaagga acttcacgag caagtactgc tccaactgcg
    26761 ccttttttcg cccagaaatt gatttgacgt gcgctcgtta ctaatgtttc agcatcataa
    26821 atatacggta tacgcaattc aggatttttt ttcatcgtat aaataatacc tgtatcacca
    26881 actgtgatca catctacgtt tatttctttt aaaaaagcca aatattcagg tacatttttc
    26941 attttttcag gatgaatgat cccatttaca gcaatattcg cttttttacc atacttgtgt
    27001 gctaattcta ccaattgtcg ctgttcttct cgtgtaaatg aagcaggcag acgcaatcca
    27061 aattgctctt caccgaaata tagtgtatct gcacctgcag caagcaactg ttctgcttgt
    27121 tcaatagttt ctactgtggt tatgagttcg atcataattt tctcctccta caacttcata
    27181 atagtagcat agctctaaaa actttccaat acttcttcta tttaaagtcg gatttttgaa
    27241 aattatttca caaaaaatca aaataaagtt cgatatattc tgtccaaata taaaaagaga
    27301 ttgtgacaaa aatcttgtca caacctctta atcaagtctt actattataa aaattcagct
    27361 acacaggcca agtaactata gggaacctac tgtttccttt ttaggtccgc gaacattcaa
    27421 tgtgatctta ccttttgaag cacctactgt tacgtggtca cctaaacgaa tttctccgcc
    27481 aagcaaagcc tcgctcaatc gatcttcaat ttctttttga agtgcgcgac ggattggacg
    27541 tgcaccatat tcaggatcaa atccagcttt cccgatcaca tcgattgcag atggtgtgat
    27601 tttcacttga atatcttgtt ctttcaatcg tttgataatc gctttgctca tgatttggac
    27661 gattgtatgg atttcatctt tactcaatga atggaagacg acagtctcat cgattcgatt
    27721 caagaattca ggtcggaaag ctttctttaa ctcttctaag atacgttttt gcatggcttt
    27781 gtgatcttta gtaacatctt ggacattgaa tccgacattt ttttcttctc gaatttgtgt
    27841 cgctccgata tttgaagtca taatcatgat tgtatttcta aagtccactt ttctaccttt
    27901 agaatctgtt aaatgaccat catccaatac ttgcagaagt aaattgaaaa cgtctggatg
    27961 tgctttctcc acttcatcta agagaatcac tgaatatggt ttttgacgaa ttttttctgt
    28021 caactgtcca ccttcatcat accctacgta tcctggagga gacccgatta atcggcttgt
    28081 actgtatttt tccataaatt ctgacatatc aacacggatc agggcatctt cacttccaaa
    28141 catgacttct gaaagtgctt tggccaattc tgtttttccg acaccagttg gtccaaggaa
    28201 catgaatgaa ccaattggac gatcaggatc tttcaatccg cttcgtgcac ggcggatcgc
    28261 acgtgaaact gctttgacag cctcatcttg accaacgaca cgttcatgca gaagtccttc
    28321 cagttctaac aagcgttcgc tttctttttt ctccaactgc tgtagaggga caccagtcca
    28381 ctccgataca actgtagcaa catcttcttc tgttacacga tcagaataac cttgctcctg
    28441 ttttgcttca attgcagaaa ctttttgcag ctttttacgt agtgcttttt cttgtctgcg
    28501 taattgagca gcattttcaa agtcttgttt ttggatcgct gcttcctttt cttgtactag
    28561 ttcatcgatt tctagtttga tcacagttga ttttgttaag tgatcggttt gatccaaacg
    28621 gactttcgct gctgattcat cgatcaaatc aattgcttta tcgggtagtt gacgagaagt
    28681 gatataacga acagaaagat tgactgctgc tcgtacagct tcatctgtga tctccactcc
    28741 atggtgctct tcatatcttg tacgtaatcc ttgaagaatc acttctgctt cttcaggagt
    28801 tggctcatct acttgaatgc gagcaaaacg tcgctctaat gcagaatctt tttcgatata
    28861 tttttgatat tcatctaggg tcgttgctcc gatcgtttgt aattctcctc gagctaacgc
    28921 cggtttcaag atattcgaag catcaattgc tccttctgct ccacctgcac caattaaggt
    28981 atgcaattca tcaatgaata agattacttg accatcatta tagatttcgt cgatgacctt
    29041 tttcattcga tcttcaaatt caccgcgata tttcgtacca gcaaccaaag atcccatatc
    29101 aagcatcatc aggcgctttt cttgcatgtc ttctggcact tcacctaaaa tgattttttg
    29161 agctaaccct tcagcaatgg ctgttttgcc tacaccaggt tcacccacca gtacagggtt
    29221 attctttgtc cgtcggctaa gaatctggat aagccgtttc acttctctcg aacgaccaac
    29281 aactgggtcc agcttgtttt cttttgctaa tttcgtcaaa tcacgtgcta atgaatctag
    29341 cgtaggtgtt ccttgcggtg tttgccgttt atcttgtaca tttacatttc tacgacgatt
    29401 cattcctttt ccagcagaat caccagctag ccccattttt tgtttcagaa cttgacgggc
    29461 ttttgaaagg ctgataccta agttgatcat gattcgagta gccatgatac tttcatcttt
    29521 taataagctc atcaaaatat gttctgttcc aactttttga gaaccagttt tcttcacttc
    29581 gctgctagct aaagcaaaaa cttgtttcat tcttggtgaa tatggaagaa agactccttt
    29641 agggtaagaa cggataccac cataaccaat cagatgttca atttcttcat aaatttccgt
    29701 ttcattcaaa tccatttcac gtaatacttt tccagcaatt ccattttgct cgaccactaa
    29761 ggctagtaaa acatgttctg aactaactga ttgatgtttc aaccgttttg cttcttcttg
    29821 tgcaatttct aatactgctt gggctctttc agtaaaaagt tcttccatct aatctcactt
    29881 cccatcttca taacttaaac gttccagaaa tgctcggaga atccgagcac gtgtatgatc
    29941 ttcatattca ttgaaattca atgtgttttt tgcaattgca ctcaacatca agtttccttc
    30001 tttttttgta attatttggt cttcataaag tttttgaata atagcaaaag catttttctc
    30061 actgattgta tcatcaaata actgatttat ctgctcaagt aattgttttt tatctgaaat
    30121 ccttactttt gcaattcgta tataaccacc accaccacgt ttgctttcta ctagataacc
    30181 acgttggatg gtaaaacggg tattaatcac atagttgatt tgtgaaggaa cacagttaaa
    30241 gaggtctgcc atttcaatcc ttcgaatctc gatcatttca ctattttcca gaattttctt
    30301 tatatatgat tcaattaaat ccgaggtatt ctgatgtgcc atcttattca ttccctttga
    30361 ctaatattga ccttaattat aacgaatttg ataatagaaa gaaagaagaa cgctttttgt
    30421 taaataaatc ctttagaatc aacaaaaaaa aatacggaat atactattcc gtatc
//
LOCUS       DAZKCY010000025        28044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000025 DAZKCY010000000
VERSION     DAZKCY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.025"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..1181)
                     /locus_tag="ACG8XC_001002"
     CDS             complement(78..1181)
                     /locus_tag="ACG8XC_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFP9190717.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1810..2280)
                     /locus_tag="ACG8XC_001003"
     CDS             complement(1810..2280)
                     /locus_tag="ACG8XC_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFP9190718.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGLFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            2610..4250
                     /locus_tag="ACG8XC_001004"
     CDS             2610..4250
                     /locus_tag="ACG8XC_001004"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFP9190719.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            4404..5726
                     /locus_tag="ACG8XC_001005"
     CDS             4404..5726
                     /locus_tag="ACG8XC_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFP9190720.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5794..6228)
                     /locus_tag="ACG8XC_001006"
     CDS             complement(5794..6228)
                     /locus_tag="ACG8XC_001006"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9190721.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(6293..7129)
                     /locus_tag="ACG8XC_001007"
     CDS             complement(6293..7129)
                     /locus_tag="ACG8XC_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9190722.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSIAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSGGAVVGGIAGNIVFEVFLRLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWIWYLICGGILGFLASFLGIGGGPI
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSGFMRFDLSMLFYIIPAAVI
                     GGLWGAKFSRILSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(7703..8008)
                     /locus_tag="ACG8XC_001008"
     CDS             complement(7703..8008)
                     /locus_tag="ACG8XC_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFP9190723.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(8033..8464)
                     /gene="ruvX"
                     /locus_tag="ACG8XC_001009"
     CDS             complement(8033..8464)
                     /gene="ruvX"
                     /locus_tag="ACG8XC_001009"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP9190724.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(8461..8727)
                     /locus_tag="ACG8XC_001010"
     CDS             complement(8461..8727)
                     /locus_tag="ACG8XC_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFP9190725.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(8982..9707)
                     /locus_tag="ACG8XC_001011"
     CDS             complement(8982..9707)
                     /locus_tag="ACG8XC_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFP9190726.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(10068..10138)
                     /locus_tag="ACG8XC_001012"
     tRNA            complement(10068..10138)
                     /locus_tag="ACG8XC_001012"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10104..10106),aa:Gly,seq:ccc)
     gene            10255..11007
                     /locus_tag="ACG8XC_001013"
     CDS             10255..11007
                     /locus_tag="ACG8XC_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP9190727.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            11023..11502
                     /locus_tag="ACG8XC_001014"
     CDS             11023..11502
                     /locus_tag="ACG8XC_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP9190728.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(11753..13333)
                     /locus_tag="ACG8XC_001015"
     CDS             complement(11753..13333)
                     /locus_tag="ACG8XC_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFP9190729.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(13326..14069)
                     /locus_tag="ACG8XC_001016"
     CDS             complement(13326..14069)
                     /locus_tag="ACG8XC_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190730.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(14245..15021)
                     /locus_tag="ACG8XC_001017"
     CDS             complement(14245..15021)
                     /locus_tag="ACG8XC_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9190731.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(15269..16105)
                     /locus_tag="ACG8XC_001018"
     CDS             complement(15269..16105)
                     /locus_tag="ACG8XC_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFP9190732.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(16116..17045)
                     /locus_tag="ACG8XC_001019"
     CDS             complement(16116..17045)
                     /locus_tag="ACG8XC_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9190733.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            17237..17590
                     /locus_tag="ACG8XC_001020"
     CDS             17237..17590
                     /locus_tag="ACG8XC_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP9190734.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(17664..18320)
                     /locus_tag="ACG8XC_001021"
     CDS             complement(17664..18320)
                     /locus_tag="ACG8XC_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP9190735.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            18546..20480
                     /locus_tag="ACG8XC_001022"
     CDS             18546..20480
                     /locus_tag="ACG8XC_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9190736.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(20566..21789)
                     /locus_tag="ACG8XC_001023"
     CDS             complement(20566..21789)
                     /locus_tag="ACG8XC_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9190737.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGHSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(21783..23654)
                     /locus_tag="ACG8XC_001024"
     CDS             complement(21783..23654)
                     /locus_tag="ACG8XC_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFP9190738.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(23675..24793)
                     /locus_tag="ACG8XC_001025"
     CDS             complement(23675..24793)
                     /locus_tag="ACG8XC_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9190739.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(24876..25709)
                     /locus_tag="ACG8XC_001026"
     CDS             complement(24876..25709)
                     /locus_tag="ACG8XC_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9190740.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(25702..26619)
                     /locus_tag="ACG8XC_001027"
     CDS             complement(25702..26619)
                     /locus_tag="ACG8XC_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9190741.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(26846..27970)
                     /locus_tag="ACG8XC_001028"
     CDS             complement(26846..27970)
                     /locus_tag="ACG8XC_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9190742.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         8570 a         5607 c         4781 g         9086 t
ORIGIN      
        1 ttagtgacat ccttgaacta taaaaatagg agtacggcat catctgccgc actcctattt
       61 ttttcgtcat ttatttctta aataaaacga ttctcacgca attttatttt tttgatatct
      121 tctactgttt gtgttccagc taattgcatg accatttcca attctgtttt aaagaaatca
      181 aacacttgat ggactcctgt acttcctcct aaagacaatc cataaataac gggtctgcca
      241 atagcaacaa gatcggcacc tgatgcgatt gctttgaaaa catgttgtcc acgtcgtaca
      301 ccgctgtcaa acacgattgg tacacgttta tctactgctt ctgccacata ttgcaacgaa
      361 tcaaacgcag caggcccccc atctaattga cggccaccat ggttagatac ccaaattcca
      421 ccagcaccag attctaatga tcgatagaca tcttcttctg attgaacacc tttcacgtag
      481 actggcaaat cagaatgagc agcgataaat tctacatctt ttggactaag tttttgtttg
      541 gaagatttat atacagcatc catcgtttgg cctactcctg attgataagc ctgaacgatc
      601 ggcattggca gcgggaaggt aaacccgttt ctgcggtctg tttcacggtt tccgccaact
      661 gttgcatccg ctgtcaatac gattgctttt gctccattac gcttggccac ttcaagaata
      721 tccaaattga ttccattatc tttgctcata taaaattgaa accattgcgg tgctttctcc
      781 ccgcctgctt ctcttatctc ttctaaagta caagaagcgt aagaacttgc tgtatagatc
      841 gtcccaaaat ctgctacacc ttttgcagaa gctttctccg ccttcacatg agctaatcca
      901 tgagcagcaa ctggtgccat gatgattggt gcagtcagca tttcttcgtc aaaatgtgta
      961 gttgtatcgg gtaattctac atctcttaat acatgaggaa taatcaaacg atgattgaat
     1021 gcccgttcat tttcttgata agtaaataag tcgccagcac cactgctgat gtaaccataa
     1081 ccaccagctg gaatcacttg tgaagccgcg atttctaaat cttccatatt gataaaatct
     1141 actattcctt catttgtacc tgcttgatat gttttttcca tctcaatgcc ttctctctat
     1201 aaaatttgaa tgatgaacaa tatgatagcg tttacacttt ttatattact cttcgacatt
     1261 gatacaggca aataatctgt ttacatttca gccattccag atgtcactac tctggaagaa
     1321 atgattgtaa taaaaataga taaaaaaccg atgggacttt gacaaaagta gtgtcaaagt
     1381 cccttttttt gaataaacgg tgttcaaaag ccattttctc ggtattaagt caaatttatg
     1441 caaaaatgtg gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt
     1501 ttcacaacct ctcgataaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
     1561 tggcaaaata tgaggaacta ttttgccatt tgtctctcta atactcaggt cagaacgttg
     1621 acttcacagt ctcttcctta aacggtgttc aaaaacttgc atctgcggta ataagcccaa
     1681 ccaaacgaaa aataggagga gctatttttg tttggctgtt cttattactt gatgcagggc
     1741 aggtttttca cgacctctga ttcacggtgt ttctttttca tcaacatttt tacgaaatac
     1801 tgtggaagac tattgaaata agtgtggatt gtttctttgt gtttcaatcg tttcttgcca
     1861 ttcttcattt aattttggat taccgttttc ttccttgaac tcataaggtc ctaattcaaa
     1921 aagcccagac agtatttgat tcacgatttc atatagttca gtgtgatcag acacatgatt
     1981 cttgaagatc atctcattat caccacgttt gaacctcaca ttcgggctct catgatgaac
     2041 ctctatgcat ttttctcctt tttttaactg acggacttct gaaaacatct cataaatatc
     2101 ttctccagta aaaccttcag caagcgccag actgtccaca agaccgccta ctgtcagttc
     2161 agagattatt gcttgattgg aatctaattt taatgtatag cttgtcgctt ctactacgcc
     2221 acatgcattg caaccgcctt ctgatagttc gctttttcta atcaattgaa cttctttcat
     2281 tttctcttcc tcctcttaat tttctactta tgtattttta tcacctgaca taggtgactc
     2341 ctatcaacgc tatcatgtgc ttttttcctc agaaatccta tttatccatt ccatctatcc
     2401 cttattcttc tacttttctt tctctagcat ataagagata cgcatagtat tcaagcaagg
     2461 attgtcgata taaaaagaac aaatactgct attttgattt cttttggcta tattacatat
     2521 aaataaaatt tatcatacct gctctttttg actatatcat catctgactt ctatacttcg
     2581 tttgtattaa aacttaggag gaatcattca tgcttacagg gttaaactta ttaaataatc
     2641 cttttctaaa taaaggaaca gcttttacaa aagaagaacg ggcaaaatat ggaatcactg
     2701 gtatgcttcc aagtactgtc caaacactgg aacaacaatc tgttcaagct tatggtcaat
     2761 acttaagcaa acaaacagat ttggaaaaac gtatcttctt aatgaatcta ttcaatacga
     2821 atcggacatt gttttataaa ttgatgggcg aacatttagt tgaattcatg cctgtcgttt
     2881 atgatcctgt cgttgcggat tcaatcgaac aatacaacga aattttcttg aaaccgcaag
     2941 atgctgcatt tctttcaatc gacgaaccag aaaacatcaa agcaagtttg aagaacgcgg
     3001 cagatggacg agacattcgt ttgattgttg tgactgatgc agaaggaatc ttaggtatgg
     3061 gcgactgggg tgtgaacggt gttgacatcg ctatcggcaa attgatggtc tatactgctg
     3121 ctgctggtat taatccagca caagttttgc ctgtatcgat cgacgctggt actaataatg
     3181 aagaattatt gaataatccc ttatacttag gcaatcgtca tgctcgtgta gaaggcgaaa
     3241 cttactatga attcatcgat caattcgttc aatcagctac cgaattattc ccagaattgt
     3301 tattgcattg ggaagatttt ggacgaggaa atgctgcagc gatcttagaa aaatacgaag
     3361 atcaaatcac tactttcaac gacgatatcc aaggtacagg aattgttgtc ctagcaggtg
     3421 tattaggcgg attgaatata tccggtgaac aattgaaaga tcaaataatt ttgacatttg
     3481 gtgctggtac tgctggtgtg ggtatcgcaa acatcctttt agatgagatg atccgtcaag
     3541 gtgtacctga agaagaagca cgccaacatt tctatcaagt agataaacaa ggtttattgt
     3601 ttgaagatac agaaggattg actcctggtc aaatcccatt cgctcgcaaa cgctcagatt
     3661 tcactaatgc tgatgaattg acaaacttag aagcagttgt aaaagcgatc catccgacaa
     3721 tcatgattgg tacttctacc caacctggag cattcactga agaaatcgtg aaagaaatgg
     3781 ctgctcatac accacgtccg atcatcatgc ctttgtctaa tccaacaaaa ttggcagaag
     3841 caaaagcaaa agatttaata gaatggacgg acggaaaagc attagttgga acaggtatcc
     3901 ctgctgctga tgttgaatat aatggtgtaa cttaccaaat cggtcaagca aataatgctt
     3961 tgatgtaccc aggtttagga cttggtttga ttgcttcaac agctacacga gtaaatgctg
     4021 aaatcatttc tcaagctagt cgagcattag gcggaatcgt tgatgtaaca aaaccaggag
     4081 cagctatctt gcctccagta gcaaaaatca ctgaattttc tcaaacgatt gctgaaactg
     4141 tcgcaaaatc agttgtcgca caaaacttga atcgtgaaga aatcacggat atcaaagaag
     4201 cagttgaatc tgctaaatgg gttcctgaat ataaatcttt agaagattaa gatcgttata
     4261 gtaaaagaac atacagaaac tttgtatgac gtctaaaaag aaaaaagcga ctgcggcaaa
     4321 acagcggcca gccaactcct gctcttttgt cgcttttctt tggctttttt catatgatac
     4381 agaataagaa aggatctgta aaaatggcac aaaaaacaac aactgcaact gcagttgagg
     4441 aaaagaaaaa agaaggtttt tggtccacaa aaatcaccgg cgtaagtttg ccactttatt
     4501 tgatcatggt ccttgtcctg atcattgcaa tggctttagg gaaattaccg acgaatatga
     4561 ttggaccaat cgcagttttg gttattttcg gtaatttatt tcattttatc gggacaaaaa
     4621 tccctatcgt caaaagttat ttgggtggtg gttccgtttt tgcgatcttc gcttctgctg
     4681 cgattgctac atttgggatc ttacctgaat atgtagtgaa atccacagaa aactttgtca
     4741 gcaatatggg ctttttggac ttttatattg ctgccttgat tactggtagc atccttggta
     4801 tgaaccgtaa tctattgatg aaagcttctg ttcgttttat tcccgttgca cttatttcaa
     4861 tggtcgtctc cttttttgcc gtaggactcg tgggcatgct tatcggaaat ggttttgcaa
     4921 attccgtttt atacatctca ttacctgcga tggctggtgg tgttggagcc ggagccgttc
     4981 ctttatcaac gatttatgca aatgttttag gtacggatgc ttcttctatc atttcccgcc
     5041 tgattcctgc tacagcaatg acaaatgtct tagctatcat cggtgcagca ttagttgctc
     5101 gtgctggaca atcaatgcca aaattcaacg gcaatggtaa attaatggaa aaaggcgatc
     5161 ttggcgatgg aaaacctcga actgtgaaac cttccattcc acagttaggt gttggtttga
     5221 tggtatcatt gactttcttc atcttaggac aaattggtaa ctattttgtt cctaaagtcc
     5281 atgcatacgc atttatgatt attatcgttg tcatctgtaa aatcgccaac ctattaccag
     5341 agtattatga agatgcagct atcatgttca ataacttgat tgtcaaaaac ttgacagcag
     5401 ctgttcttgc cggtatcggt attgcgttgc tgaatctgaa tgtattagct tctgctttga
     5461 catggcaatt tgttgtctta tgtctgacaa gtgttatcgc gatttcagtt gtttcaggat
     5521 ttgtcggtcg tttgtttggt ttatatccaa ttgaatcaac tattactgct ggcttatgca
     5581 acaactcgat gggcggaaca ggaaatgtag ccgtcttatc tgcagctaat cggatggaac
     5641 ttattgcttt tgctcaaatg ggcaatcgac ttggtggtgc catcgttttg atcatctctg
     5701 gtttcttgat gcaattactt tcttagtcaa aaaaacaaaa atacctgatc atcaagtatc
     5761 ggcttggtga tcgggtattt ttttgataaa gatttacagg attttcaaaa atcttttttg
     5821 cattttatcg gaatgataac cgatgtgttc ataaaattgg tgtgcttcta ctctgtattc
     5881 tgcagaatta agccttattg tcgttattcc tcgttttttc gcttcttctt ctacgccagt
     5941 catcaaagcc tttccgatgc ctcttttttc agcatattta gacacagcaa gtgccataat
     6001 attgaacatt ggatcgctaa aaatcgattc ataggactcc gcttgtacat agccagctgc
     6061 ttttttagaa gtaggatctt cataaaccaa gaaaaaattt ttctctggct ccttcagcag
     6121 tttcttcatt tgtctcaccg taacggttaa tggtatatca taccctaatt gatccgcatt
     6181 gatttcacgt agtgcttctg catcttctaa acgcatttcc cgtaacatcc aaatcaccag
     6241 ccaatttctg attttttaaa atgaattttt ttatcatagt atactctttt tttcatttag
     6301 aacaatatgt ttcagtgatt ttgacttgcg ttagtctaga ttctcctaat gtttgtaaga
     6361 tgacaaaggc attgtaacaa ttgaccaaaa gaaccacgag gacaattact tgaaaaataa
     6421 aggtcacttt ttttggagac aaaatcctgc taaattttgc tccccacaaa cctccaataa
     6481 cagcagcggg gataatataa aacaacatag ataaatcaaa gcgcataaag ccacttgtta
     6541 aagcaatcgt tactaattta gccagttgag aaaagaaaat cgtagataga gaatacagcg
     6601 ttgcttcttt tattggcaag gcaaacatca gcatcagtaa tgaaacattg atgggtccac
     6661 caccgatacc taaaaagcta gctaaaaagc ccaagatccc cccacatata agataccaaa
     6721 tccatgacgt tagatgaaat tttggctgat gatgcttggt gtagaagaac gcaaaaacca
     6781 aggtcaatac cgtcaagaaa atttggatca gctgcacatg cttttcattt tcaaacaacc
     6841 gtaaaaaaac ttcgaataca atgttaccgg ctattcctcc aactacagct ccaccagata
     6901 cccaaccgac aatctgccaa ttcagcgatt tcccacttga tacttgtcgc actgtcgaaa
     6961 caatagacat cgtgaatact gcaaccgttg agtaaaaaga aatacctgca atagaatggg
     7021 caccgataag atcaagaatc ggtttgatca acacgcctcc gcccatgccg gaaatagcgc
     7081 caactgtatt tgccagaacg atcaccacaa aataaatcag accaatcata tcatcttgta
     7141 tatcctttct cttatttata tcttttagcg atttttactc tttgcttata ttatacagta
     7201 aaagaaacaa acgaggcttc gaaagctttt ctacttttta aggaaatgta gtactaaacg
     7261 aaaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
     7321 ctcctcataa ttttcgtttg gttgaactta ttaccgcaga tgcaagcttt tgaacaccgt
     7381 ttaagaaaga ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca atttcaaaaa
     7441 acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttggc aggagaacac
     7501 cgtttaagga agagactgtg aagtcaacgt tttgacctga ttattagaga aacaaatggc
     7561 aaaatagttc ttcatatttc gccgtttttc gagcgaatgc cgaggggcag ctgattgaac
     7621 atcgtttatt tggaaaaagg gattgtgaca agacgtttgt cacaatccct ttttttatat
     7681 cttattaatt cattcttttg ttctattctt cttcttccgc catgaatgta ttgaatactt
     7741 cttcaatcat gtcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat
     7801 cttcgttttc gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag
     7861 caggatataa aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagtagga
     7921 tttcatataa tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcat
     7981 ggtcgtggga atggttatgt tctgtcataa gattacctct tttcttttat tattaaaata
     8041 attgcttacc tgagccgtct aaataatttt gtaagatcat cacagctgct aatttatcga
     8101 tgactttttt tcgtttggct cttgaagtat ttgcctgctc tactagcata cgttctgcct
     8161 gaacagtggt taatcgttca tcttgataat caactggcaa ggagaatagt tctttgatct
     8221 tttctccgta tgacatggaa gcttctgccc ttggtccgat ggaattgttc atatttttag
     8281 gtaatccaac tacaaaccgg tctacttcat attgtgctac taattccttc aaccgatcaa
     8341 agccaaactc ttcttctgct tcattgatcc gaatgatctc tactccttga gctgtccagc
     8401 caaaggggtc actgatagca acacctactg tcttagaacc gacatccaag cccattactc
     8461 tcataaattc actccatgat ttgagaggta gtccttgaca attacttcca tgatctcatc
     8521 gcgttcgtga cggcgaatca aatttcgagc atcacgataa cgaggaatgt aggccgggtc
     8581 tccagaaagc aagtagccga caatctgatt gattggattg tagccttttt cttccaaagc
     8641 ccgataaaca atcgctaatg tttcgctcac ttcttttttg cgactatcgt caaaaccaaa
     8701 acgtacggtt tcatctgtaa aacccatgat tctacacctc tttcccgcaa ggtaattcca
     8761 gcttaccttc tacaacctta ttttactcga agaaagggaa aactacaaac aaatattcca
     8821 aacttccgaa taaattaaga aacactattt gtatgttttt ttcctttttt ctgtagcttt
     8881 atcaaaagta ctcttttcat aaagtatacg ctatatttct ttgaatgtat gtttttacac
     8941 aattttttca taatattcat aaaaaattcg aatagctgct actaattttt tgtttttgtt
     9001 gcttgtcctg tctcataatt gatcgtgtag ccatcttgaa cattgtagat aaagacattg
     9061 aattcaattt ttttgtcttc aatgctttct gcttccaatt gcactcccct ggctaccagt
     9121 tcattcccct caaaatgagg agtgacacgg tagcgtacat gatgatttgt ttctttgata
     9181 tatgccgcaa tatcattttc gtgagcaagc atgtatggag cattcaacga acgtgtccct
     9241 gtcatcaagt ttttaatgtt gttattttga ccagtcagct ggaaaccaat taaatggctg
     9301 cgattatata accattgccc gtcacttaat ttcttttgtt tccaacctgt cgggtcaata
     9361 tacaaaggct ctctcttttc agtagggaac aaatcttttc caagcatagc attggcttgt
     9421 cctactctgt tcaatttatc aatatttgaa tagctttccc atgttccttt ggaaaggcta
     9481 agatcctctt ttgtaaattc cggctgattc ccattgattt ctatttcttg atgttttcca
     9541 tcatattctg gcacatccgc tacttgagaa gcttgtggtg tggaatcaaa aatagttgta
     9601 atctgctgaa agatatctga atctttttgt gtggtggaaa caccgaatgc ccctgcaatc
     9661 acagcagcga cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt
     9721 tactaacagc tccctaaatc ttaacatatg ttcccctata aataaatact agaaagaaat
     9781 agaaacagca aaaaataaaa cctgttttaa gatggcttaa aaatacaaaa aagagacgga
     9841 ttcctcgtct ctaatcagtc accactctat ctgtaaggta tttattatta ttcaaaaatt
     9901 ttcttcatac tcaagacaat acactgattt cacaaccgct tggtaaacgg tgcgcaaaag
     9961 cttgcatctg cggtaataag tccatccaag ttaaaaacat aagaagttgt ttttgcttgg
    10021 ctgttcttat tacttgttgc agagcagctt tttcacaacc tattttaagc gggtgacggg
    10081 aatcgaaccc gcgacatcag cttgggaagc tgggatttta ccactaaact acacccgcaa
    10141 tacaaaaaca gtatacgcct cttttattcc ttcctcaagt ttttttattc tttacgcttc
    10201 tggtatgttt tgtagtaaaa taaaactaat caatcaatta ttggagaaga tgctatgacg
    10261 ggaaaagaat tagatctgtt gctggacact tgtttactcg ctgggaaaat catgatggaa
    10321 agcaatgcgg agatgtatcg ggtagaagat acgatgagtc gaattgcttt agcttctgga
    10381 aatttccgtt tggtcagcta tgtcactcaa accggtctct ttattggtct tgatcgtacc
    10441 tcgaccatcc gaatggaaca aattaccaac cgttcgatta atttagaaaa agtagtaaaa
    10501 gtaaatgatc tttcacgtaa atatgttgct tcagagctaa cattagaaga actttactgc
    10561 gaactgcaag caatcgaccg agaccgttct ttctttcctg tctggttgca aatcgttagc
    10621 gcggcagtga tcagtggaac aattatggtg ttatttggcg gaaagatgac cgatttacct
    10681 gtgactttag tcattggagg attgggatac gcagcttatc tatatagttt gaaatttttt
    10741 agaatcaaat ttttatcgga atttttatct tctttattga ttggcatagc cgctatctac
    10801 agcgtacgtt ttggcttagg aagcaatcag gatctaatca ttatcggatg tgtgatgcct
    10861 ttagttcctg gagttcaaat tacgaacgct atccgtgatt tgctagctgg acattatgtc
    10921 tcaggagttt ctcgcggaac agaagcgatg atgactgcta cgatgatcgg ttttgcgatt
    10981 gcttttattt tccagctttt ttattaggaa aggaacacaa gtatggggac gatatttatt
    11041 cagttttctt ttagtttttt agcgactgcg gcatttgccg ttattacaaa tgtacctagg
    11101 cgttcattga tttgttgtgg tctttctggt gcttttggct ggatgatcta ctggatctgt
    11161 gttcaaatag gcggatctgc tgcttttggt tcattgcttg gttctttagg agtagcattc
    11221 atcagcgatc ttttctctaa acgtttgaaa atgcccgtaa cgattttcaa tattcccggg
    11281 atggttcctc tagtccctgg tggtttagct tatcaagcgg ttcgaaatct cgttactgaa
    11341 tcctatcgag aagcggctca ttatgcggtc caagcaatta tgattgctgg agcaatcgct
    11401 ttaggtcttg tattatcaga agtctttaac cataacatcc gtaatttcaa agtaaagcgt
    11461 gacgcaatca tgatgaaacg aaaaaaacgg ctttttaaat aaacgaagtg taacaaagaa
    11521 aaggttgtga aagagacgtt cagccttgag cgatgacaaa atatctcaca aacattgcga
    11581 caagtaaccg caggagcaat aagaagtaat tatgagtatt aaaaaaagtt ttgtaaaaat
    11641 agcttctcac atttttacat aaaattgact taatactgag aaactgactt ttgaacaccc
    11701 tctatctgta aaaaaggggc tgcgacatga tttttgtcac ggcccctctt ctttattcaa
    11761 attttttcca aaacgttcgt tgataataaa cgatccatag agcggaaagt attagaacta
    11821 aaccgatcac aggaatattc actaaccaaa atggataaaa caaagccaac attccgtata
    11881 agacgataag aataatactg atgatcaatg aacccatcat agttgcgact aaacccaaac
    11941 ttcctgaacc tctgttaaat aattggttaa tattggtcca gttcagcagc agcaaccgat
    12001 aatctctagc gaaataacgt agtgacaata agtagcagcc gaataaagaa ccaaatagca
    12061 agctagggat aaaaaacaaa ggcagctgaa aagacagtcc gcttaataat gcgatcccac
    12121 cagttaaaaa actctgcaat atccatccga atctgaactt ttcttttaaa tattggttca
    12181 tagagattgg caaagaacgg ataaacagaa aattttcttg atccaaagaa ataagattgc
    12241 tgataaaaga agtctgatta actgttaaga aagccaatgc gatccctacc aggaaaacaa
    12301 caccgattaa cttattatct aaatcagtta attttatttc gcctcctagg gcaaaagcaa
    12361 gaataaaaat gattggagtt aacaaggaag aagaaagaac ttgcataatc aaattaggtt
    12421 cttttactaa ttgagcattg taactaaata aaagctgatg cacattttgg ttctttttat
    12481 gggttctttt tattttgaac tcactgtttg aatcagtcag ttgctctgta agctttggta
    12541 ctatcagctg ttttacagca aggaaaaatc ccagagccgt gatagctagt attccccaac
    12601 ccaataaacc ttgttgagaa agtggctgac tggcagcatc atagaatggc aataatatcg
    12661 aaatcggatg ccgatctgtt aactcatttt caatagtccc cgtttgatga ttcatccaca
    12721 tgattccagc aaatgcgatc agcatcgagc tgccaagcaa gatactagaa actgtttttc
    12781 tatgtttttt aaacactgct gttcgagcca aaccaaaaac aatcaaacta gccagacaaa
    12841 aaagaataat caaaaataga gaaaacaaaa ggattgagat aatgatagta agcaaaatcc
    12901 ctagatcagc tcgccatccg gttaaaataa acatgactag aagcggaaaa acaaaaggaa
    12961 tgatggtcaa agcaactacc aaaatctttg ctgaaaaaac ctgttcttgt ccaaaaggca
    13021 gaggcaaaaa tacttgcagg tcatttcctt caaaaaagat attataaatg actgatatac
    13081 tttgagttaa acctaacata ccaaaaagtg ccacgtaata agtaaagaaa cctggcaatt
    13141 taacaaagtt catagcaaac atagcaaatc cgtaaatcag taaaaaaatc agccctgaaa
    13201 aaagattttg agacaaaaga tagatagtta atcgctttcc cttttttcct ttctctcttg
    13261 ctttattcgt gagctgtgga ttaacatata aaagattaac tcgaattaat tctaataatt
    13321 gtcgcttata catacggtcc atctcctgtc gcttgccttc cagccatttt caaataaatc
    13381 ttttctagcg tttcatcagg tgagccattc aataattcac taactgcccc ctgataaatc
    13441 aattcacctt tcttcaaaat agccagttta tcgcataact gttgtgcggt atccaataca
    13501 tgtgtagaaa aaatgactgt ttttcctttt gcggcatgag ccttcatcat ttctttcaaa
    13561 tcaaatgctg cttgaggatc aagtccttgc aaaggttcat ccaataccca aatatctgga
    13621 tctggcagca acgtgccgat caaaatcgtt ttttgacgca ttccatgtga aaaacttgcg
    13681 attgtttcat tttgatgact atacatatca aaaagcgctg tcaattctgc taaacgcttt
    13741 tctttttttt gtgtgtctaa ttcataggca gctgaaatca agtcccaata ttctccagcc
    13801 gtcaattgta aaaaaatatc tggggaatca ggcacataac cgattttttg tttgatactc
    13861 aatcgatttt ccgtcaattt cattccatct actaaaatcg ttcccgatga tggttcaata
    13921 atactaacta aacttttaat cgtagttgat tttccggcac cattatgtcc taaaaatcca
    13981 aaaatttccc cttgtcttat cgagagagac aagtctttta atgcttgttt tttcccgtaa
    14041 cttttactta cattgatgaa ttcaatcatt tctgtactcc ttttgtttgt tattactttt
    14101 tattgtaccc ttcgttattg gtggagtaaa tgtcaaaaag agtgcataac aaaaaaggat
    14161 agaaacgact aggctgctac ttgggttgtt ctcttctaca ggaaaacact cttctaacag
    14221 gtaagatatg agtctttgtt ttctttaaaa tgattcattt tctatctttt cttgcataaa
    14281 attctttagt ttatcaatac ttctctttcc catctctagt tgttctccat ttgcaaatgt
    14341 tactatttcg tttactctgc taatagatgt aatgttttca gtattaatga tataagcttt
    14401 taaatcttta cataaatact catattcgag tgatttttta tattttttaa ttgtgccgtt
    14461 tacgagaatc tcttcatctt tcaatactag gcctatttta tttttataac ctggcatgct
    14521 cattagataa aggatttcat cgtaatggat atagtgatcg ctattaccat aacggacagt
    14581 tgtccacctt tttttcgtag catgttgaac aggatagttt tctttttctt tcaataagta
    14641 actttggatg ctttttttat ttttttcata agtgaattct tttatcaaat aaccactagg
    14701 cgtaatcttt cgattaataa cagttattgc tttattcgga tgagacgtta gataaacaat
    14761 tttacacttt tcattctttt ctctaagcat ttcgcctagt tcgataccgc tataataggc
    14821 gttcaaatcg atgtctagga aaaaaatatc tgaggaatag atttcattat ttttaatgaa
    14881 ttcaacaaat tgtataacgt tcaatccctg aacgatctca aatttccaag gacttaataa
    14941 aattctggat tcttccaaaa tttctactag cattttttca aaaaccaaac tatcatccaa
    15001 aataaatata cgcgttacca taaaaagtac ctccactcga tattttacta tcaaaatcat
    15061 ttcatacaac ttttcaacat acatgttagg aataaattgt aattctcttc acaatctttt
    15121 ccaacacctt tgaaaccgat atcaaatact ttcaggatgc tgcttctaaa ataaatatca
    15181 aacaaatgaa tacatcatgc aaaaaaagcc agacaaccga cgtccttgcg tgtcagctgt
    15241 ctgagctatg tttatttatt ttgttctttt aaaaagaacg atacacatac tgatttttcg
    15301 gagcagagac tttctcagcg tttgtaacaa ctacagatgg gatttcacgc ttgaatggtt
    15361 gataatagtc tgattggatg gttccttcta ttttatacca ttcatcattt ttaaccgtta
    15421 ctccttccgg catatgcgtc aacaaaccaa agactcctga atctgccaca caatggataa
    15481 tgccgaatcg gaacaagaag atatctgtat tgctatcgtt cggtgtttgg taaacaaatc
    15541 cttcatagct gatttttttg ccaatgaatt cgctagggta attataaatt agctccataa
    15601 cttctaaata gttttcatct gtgatcttga tagtttgctt atccccatac ttttctttca
    15661 actcattcat ttggtctaag tagtctgatt tgttgaaata aatactggta tccggcttta
    15721 agtattgtgt ctgcatctcc ggatccccta ctgattcttt acttactggg aaattaaatc
    15781 ctttggcttc tacgatcgta gtgtcgagac taactgttgg aaacaacgtc cccacgatta
    15841 ctggcaaagc tagcagtaca taagcgattc cacgttgata aggttttgac aacccatggt
    15901 catgatcgtg ctcgtgtgcc ttttcttctg cttttccgtt tttgacccac aaatacagct
    15961 gaactaaagc caaaagagca gataaaatca ttgaaagaat agctaagtag cgataatgga
    16021 cattgatata ttgatcgagt ttgccagaaa cttgcaagta catcatcaat tcgaaatacc
    16081 cagataaaat caaaaaccgc agcatatttt ccctcctaaa taaataatgt gaatccaata
    16141 acgctaaggg caatcagtcc gatcagcgat aaaataaagc gtcctgtaaa atatcgtttc
    16201 atcatcagta gatttttgat atcgaccatt gggccaaaga ccaaaaaggc agctacaggt
    16261 gctgtgccga acagacttaa caaagaagat cctataaacg catctgcttc tgagcaaaga
    16321 gacatcgtaa aggccaataa cagcatgatg ataatggcta aaatctttgt tgagccgagc
    16381 gttagcataa ttctagttgg taaataagtc tgcatcgcag ctgcgattaa tccgccaatg
    16441 atcaaataac gacctgtatc aaaaaattcg tcgattgaat gtgtcaacac atgcccagtt
    16501 tgatggatca gctgattttt tggttcattc ttctcacttt gttcttcttc atggacatga
    16561 tgatggtgat tttctgctac tttagacgtg attttttctt tcaatacagc gtctttttga
    16621 aaataggcaa gccagacacc aatcacaagg gcaacaaaaa agctgcctat cacccgccag
    16681 cttgccatct gccaagaatt gccaaatgcg ataaaagtgg aaaaaatcac gattggattg
    16741 atgatcggag cagtcagcat aaaagcaaac gctgtgtgaa ctggtacgcc ttttttaaca
    16801 aattgatgaa cgatcggtac gatgccgcat tcacatgaag gaaagaaaaa tcctaagatc
    16861 gaaccagtaa aaatagagaa gatacgattt tcaggaagcc atctcttcac acgttctggg
    16921 gtcaaaaata cttgtaaggc gcctgagata agacagccta ataatacgaa aggtaatgct
    16981 tcaataacga tcgataagaa aatcgtactc atctgcaaaa ccgaatttgg aataaaagaa
    17041 aacatgagtt tcctcctttt tatttctagt tcattcgctt tgcaaatata cgcttttatt
    17101 caaaaaaaac aagttgctct gtaaattttt acacaactct tattttttac caaagcactt
    17161 caattgactt tttctgttat tcccattaaa ttaaggtctc atacatatca cttctatcat
    17221 aaaagggaga ataaaaatga aacaaaccac taacttgact gaagttcaag acatactgca
    17281 acagtcatcc gttgccgtca tttatctttc gatgcctaat tgcagtgttt gtcacgctgt
    17341 tcgtccacga ctagaggaac ttctcacaca ttatgatatt ccggcacttc atttggatgc
    17401 atttgaaaca ccagaagtag ctagtaaatt tgaagtgctg actgcgccag ttgtcctgat
    17461 ttttcatcaa ggaaaagaag tctttcggca agcacggttc atcgacttta aaaaaatccg
    17521 ttatctacta gatgaactga cagcagaaga tgattccctt tcttatgaag aaatcttccg
    17581 aacaaaataa tcgttgtcca aacagaaaaa agcgattgaa caagtgaaag ttttgccttc
    17641 atttgttcaa tcgcttttta tttttattct gctgtatttt cggaatgcgc atttgcgtca
    17701 gaatgcaaga aatagatcaa ggtttgcaat tcattcgtca aatcaacatt ttggatcaat
    17761 acatctgacg gagcattcaa tcgagcagcg gtaaagttca agattccttt gattcctgct
    17821 tccgttaact gattgactac ttcttgtgct ctttcagcgg gtatcgttaa aattgctact
    17881 tcgatttgtt ggatcttgat ttgttctgac atatcttcca ttggataaac ggggataccg
    17941 tctacgatgc gcccaacgat ttcctcattc acatcaaaag cacaactgat acgtatgcta
    18001 ttactttgat ggaatttgta ttttagtaaa gcacttccca agttccctac accgatcagc
    18061 gcaacatttg tcaattgatc gtcgtttaat gtctttgcaa aaaagtgcat aagatcttct
    18121 acatcataac catacccacg ttttcctaat tcgccaaaat aagaaaaatc tcgacggatc
    18181 gttgcactat ctacttgaac ggcttcactt aattcagttg aagacacttt tttctttcca
    18241 gcattattta aaatccttaa atagcgataa tacaaaggca gtcttttagc tgtcgccttt
    18301 ggaataatat gatctttcac gaaatgacct ccacgtattg tctaattaca tctttgattt
    18361 ctccatcata gcagtattta taggaaaaaa tcaattccat tgctcagata ttgtgaaatt
    18421 ttattcttta ttcatttatt tatcatttct gcaaatctcg cagtataatg gaatttatgg
    18481 tagactacta attgaaaaat agttttttat ttgaggagaa ttcacaaagt agaaaagagg
    18541 attccatgat attattacaa gccaatcaga ttgctcgcca ttttggtgca gaagtcttat
    18601 ttgaaaacat acatttagaa atcagtacag gcgctcgtgt cgctttagtt ggcagaaatg
    18661 gcgctggaaa atcgacttta ttaaagatca ttgctggaat tgaagcacct gatgaaggaa
    18721 agatcgctaa aaataaaaca gcgactctcg gatacctcgc tcaggacaca ggacttgatt
    18781 ccaatgaaac agtttggaac gagatgctga aagcttttga acaagttaga caaatggaag
    18841 agcggatgcg cgagctagag attttcatta gcgaaggtac gccagacaca cctgcttata
    18901 gttcttattt aaaagaatac gaccggctgc aacacgattt ttcagatcga aacggctatg
    18961 gttatgaaaa tgaaattcgt tccgtattgc atgggtttaa attcgattct tcattttatg
    19021 atcagaaaat cgatacttta tcaggcggac agcgcacacg tttagcttta gctcgcatgc
    19081 ttttgcaaca ccctgacatt ttgattctag atgaaccaac gaaccattta gatatcgaga
    19141 cactttcctg gcttgagaat tatctgcaaa gctatactgg tgcattattg attgtttccc
    19201 atgaccgtta ttttttagat aaagtagtca atgaagtcta tgaattaagc agaagaaaaa
    19261 tgcgccatta caaaggtaac tattcgaaat atctagattt gaaagcagaa cagttagcaa
    19321 gtgaatggaa ggcttatgaa aagcagcaaa cagaaatcca taaattagaa gacttcgttg
    19381 ctcggaacct tgttcgcgcc tctaccacaa aacgagcgca aagccgcaga aagacattag
    19441 aaaaaatgga ccggctggat cgtcctcaag gggatgaaaa acaagcaaac tttctattca
    19501 gtatagaaaa gccttctgga aatgtagtct tgcaaatcga agatgccgct atcggatatg
    19561 ataatgtcac cttatctgaa ccaatcaatc tagatatccg tcgtcaagaa gcgattgctc
    19621 tagtaggtcc gaatggtatc gggaaatcga cgttgctgaa atcaatcatt caccagatcc
    19681 cttttatccg tggaaaagaa acgctaggga caaatgtcac catcggctat tacgatcaag
    19741 gtcaagctga tctgcataca aacaagacga tcctagcaga attgtgggat gagcatccta
    19801 ccactccaga aaaagagatc cgaagtgctt taggtgcttt cttattttct ggcgaagatg
    19861 tagaaaaacc aatccctctg ctaagtggcg gtgaaaaagc acgggttgct ttagccaagt
    19921 tagcgatgaa caaagaaaat tttttgattt tggatgaacc aacaaaccat ttagatattg
    19981 acaacaaaga agtattagaa aatgcattga tcgattatga cggcacactg ctttttgttt
    20041 cccatgaccg atatttcatc aatcggatcg cgacaaaagt cgtcgaactc tctgaaaatg
    20101 gcagcaaagt ctatctagga gactatgatt attatttaga gaaaaagcag gaagaagaag
    20161 aaatcgccga attgttggca caagatgagc cagaatctgc tcctgctccc acgaacaaat
    20221 ctgattatta ccaaagcaaa gaacagcaaa agcttatccg tagccttcaa cgaaaaatta
    20281 ctcaggttga agaagaaatg gcgcggatcg atgaatggat cgatcagttg gaacaagaaa
    20341 tggtcgcgcc agaaaacctg gaagatcacg taaaactcaa tgagctgaac caagatttag
    20401 aagctgctag acaagatcaa gaaacaaaat tgacggaatg ggaagaacta agcttggaat
    20461 tagaagaata tgaaaattaa cgaaaaaacc aaaacagaaa ccattcatgc tgttttggtt
    20521 tttctttttt tattttatca aacaaaaatt ccatatttac ctttttcacc aataagaaga
    20581 ccaattacta gtcattctca ctaaagcttc taaataatag taatcacccc aaatacaagg
    20641 tgtatttacc ccattatttt tgggcttatt ataaacagac tgtgttaaaa agccatcttc
    20701 ttcttccgtt gtagcatatc tttctttcaa ttgtcgcata attttccaaa cagcagactg
    20761 gtataagttc ttttctgttt tgtctaatag acctgattct attaattcta ataatccaca
    20821 aaccatgatc accatactag atgtatcttt ttcttctctg ctagaatcat caaagatcaa
    20881 atcccaataa caaatggtat cttctggcaa ccgattcata aaataattgg ctaatatttt
    20941 gcttaaatca aaatattttt gttctttcaa ataacgataa cttaatgcaa aaccgtaaat
    21001 cccccatgct tggcctctag cccaacagga attatcacta tgaccttgat gcgtacttcc
    21061 atatcttggt tttccatctt tgacatccat ataaaatgta tgataagttg aaccatcttc
    21121 tcttactaaa taatctgctg cattttgaat atgagcctga gcagcttttt tatatttttg
    21181 atttccagtt tcttcactcg cccaatacaa taatggaaga ttcatgcaac aatccatgat
    21241 cattctacct tgttgtgaag gatcactagg atctccccaa gcttgtataa ctcctgcctt
    21301 ctcccaaaaa cgctgaagta atgtttctgc agcatcaatt gaagcttcct tcatttttcg
    21361 atttcccgtt attttataat ctgctacggt tgataaagaa tacaaaaaac caagatcgtg
    21421 atgattagtg ataatttgac agtcaatcct gtggataaaa tctatatctg ctttttcagc
    21481 tgtttgttga aataaaggat cacccgtctt ctcaaataac aaccaattaa ttcctgtcca
    21541 aaatccttct gtccaaccaa tattgtctct taatggataa cgtcctttta taggacactc
    21601 gcttgggaat tgatactgac tgttactgat acgtttgatt aacctctccg tgtgtctgcg
    21661 acaacgctcg atctcattat tccattcttt ttccattaat tctttataac attcatttat
    21721 ccatttttct ggatgtatta tctcttcaaa catcacttta tcaagcattt ccccaccagc
    21781 ctttagcata attttagtct ttttttattg tttttatttt ggatagttag cccggttgtc
    21841 ccatcaaaat caattttggg acgattgttc catacttcta aactataatc ccgatcttga
    21901 tgagcaacca ctcctgttcc gagccaatgg gttccttttc caaatgtacc ctctaatcca
    21961 ggaataaaag tgttttccca tgtcatcaag tttgtattgg gcaaacccat aatagctgtg
    22021 gattttcgtt taatagtatc gtcacttaaa cagataatac ctgaaaagcc agcttcactc
    22081 accctatagt tttccttgtc ttcttctatg attattcctt ctttctcagg agaacattca
    22141 atagcaaacc ctgtctcata tagaacatat tctgtatcta attctacttt gtgtacgcga
    22201 atctgccagc cttcaaaagg aataagccaa gttctgattt ttgcctgtaa agtaggttcc
    22261 catgtagagg caagtacctc tttcttacat acaatgtctc gtacattatt tcgaacgatg
    22321 aactgtccat aggttttgat gctctcattc ttaaaactgg tcgtgatata tgtgtccgct
    22381 cgctgaatcc ctagcattga atccattgcc atttctgctt tcaattgatt cgctcgcggt
    22441 atcgaaaaac caaattgact agaataagca aattttaaat atttttcatt ggtcaatgta
    22501 tggaaatttg gccctaattg tcccgcattc aaaaaaaatg tatgtccatt atcatgttgt
    22561 atgagcaaat tggcttctga aagaagttta gttccttcac gaataggcat aggcatttct
    22621 ttagccgtcc aatactcatg gtcttcaggt agcgccaaca ataaaaaaat cttattcagc
    22681 cataatggag ataacgtact attatagggt tcagtcaatg tcaattgagg gtaggtataa
    22741 ccaatattca ggataccttt ttcatcaaat atttcttttt gcatccacca tcttatattc
    22801 cgattgacta accccttgat ttcacctaat ttaaaaggca aaagatcggc ccacaacata
    22861 gctgaccaaa agcttgaaac tgcaaaacga taaatcatgc ttcgaccaaa aggcacattt
    22921 gcaccatctt ccgcaaaaaa atgaatatag tctctagaaa aatcaatagc tcggttctta
    22981 tatatctggc taatgggctt ttctggttct agtacgctat aaatcaaacc ataataatga
    23041 aatgcaaaag gattataata atcttggcga cctcttgaac tgtctcgata ccagccattt
    23101 tgaagataac aattttctat attatcaaat gcttctttca gtgctgattc tttaactgga
    23161 taaccaaatt tttggataat tgttccgaca attatcttaa aaaattgcca attaccatct
    23221 gcacattcat attcaaaaat ttgcgagaac caatgaataa tctggctttt ttctcctgta
    23281 tctaattttt tccataataa ttgttcatga tgatataacg ctaataacat tgcgggcatt
    23341 tctaccatac gctggtctct ttcatgtatt tctccccaat aatcgggatg ggaaggatct
    23401 gttccagctt ttattcctga tagaattaat ttcaaccatc gttcatctaa ttggtcaatt
    23461 gcgggccccg ctccccataa aacccgagag aatccttcca ttcctgcaat tttatttcca
    23521 tatcctaccc cttgatgtat ataatcaatt tgtgcgccac ttgctcgata aaacggctgg
    23581 agtggttgat ttatcttttt aaataagtca aagtaatcct gtctggtcct taatagtggt
    23641 actggatgtt tcattgtatt ttctcctctt tttactacca aatagctaat tccgactgtt
    23701 ctaatcgaag taagccttca ataaaaaaat aatcagaata gatcagtggc acattataag
    23761 tcagttctct atggtatgct tctgctccgc ctccaatgat cccatcttca tccatattcc
    23821 aatttgcata gtgcttttct gtcttttgga gtatattgat agcagcttca tgatacattt
    23881 ttgatcgtac agaggaaaca tatcctgcta attccagaag accgcatgca gcgcatactc
    23941 cagcactggt atcaatattc gaatctttta cagcaaaatc aatgagagga atataaccag
    24001 ttttttcgat tttctgaata aaatttactg ctatcttttc tgctatagct agatagtacg
    24061 gattttttgt gtaacgataa cttaacgcga atccatataa agcccatgca tttcctctgc
    24121 tccaacaaga attctcattg tatccttgcc cgccaagtaa ccgaatatat ttccctgatt
    24181 ctgcatcaaa ttctgcgata tgcccggttg atccatcttt cctcactaga tacttagctg
    24241 ttgtatctgc atgctgccga gcaattttag agaacctcgg atcaccagtt tccctcgaag
    24301 cccaatacag taatggtaaa ttcatcatac tatcaataat catccaacca ggtttatggt
    24361 cattccatgc tactaaatag ttccctgaaa tattatagcg tccagcaagt accgtagctg
    24421 catgtaaccc tagttgtcga ctatgtttgt tttgagactg ttgataatgt gcaatagccg
    24481 atggtaagaa taaaaaacct acatcatgat gaacgtctga aaaatggtac agggcagatt
    24541 ctaataattg ttcttgttgt attgctgcct ctttgaactt ttctcgtttt gtcttgtgaa
    24601 agagctgcca taaaatacca ctccaaaaac cattggtcca ccagtcgatt cctctgtcct
    24661 ttattacgtc tgtatatttt ccgttgtaag aaatatatgg aattttattc cccattctgt
    24721 ctaattccgc atctatcttt tcagaagcta cattcaaaat ttttcttgac cattccttcg
    24781 tctgttcatt catggatatc ccgccattct tttaattttt atgaatgtgg agttgctcct
    24841 agaacgaact agatggttaa cattcgttat tttttttact tatttgcagc tccaatgtca
    24901 ttctccttaa atttagactg cgcccacaca taaatcaaga ccattgggat cactgaagtt
    24961 gctaaaatta gcataattgc gccataatta attggctcac ctgtactaga aaccgtcata
    25021 tttttgataa ttaagggaac cgtatattta tctttatctg aaagcatgac taatggaaat
    25081 agatattgat tccaacttcc cataaagata agtaaaccac tagtaataat cgtaggtgat
    25141 aaaatcggta cagcgatttt cgtaaaaatc acccactcat tggcaccatc gattctggca
    25201 gcctccatca gtgctgtagg aaattgactg ctatattgtc gcatcataaa cactgaagtt
    25261 gtactggcaa tcgaagggat gatcacacct gcataagtat tcgataatcc taaacgactc
    25321 ataatgtaaa ataaaggaat caatgtggta aaaaaaggca ccatcctaga tagcataact
    25381 gtattgaaca acagtttatt ccccataaaa cgatactttg ctaatgcata acccgccatt
    25441 gtagaactgg ctgtagctaa aactgtactg attacagcaa taacaatcga attccaaata
    25501 atcaagggat aattatatct cgccatgatg gcttgccagt tttttaatag catatcccca
    25561 aacttgaaat tgattccgcc acttaaaatt tcttgattac tttgcgttga agctacaatc
    25621 atatataaat atggcatcaa aaatattaag gcaccaaaca caataatgac gtacgtaaaa
    25681 attctgcttt gtatcttttg tttagtcatt tttatcacct ccgaatttaa attgaatagc
    25741 cgataatata acagatacaa acaccatcgt ccaggcaata gcagaggctt ttccaaaatc
    25801 aaccatttca aaacttgttt tatataaatt gactcctacg accaaagctg aattttctgg
    25861 tccattggaa gtcccaaaga atatatttgg gatattgaaa ttacccaagc cattgatagt
    25921 ggctaaaact gtggccatta aaatgattgg ctttagcatg gggatcgtta ataaaaagaa
    25981 ttgccgaaat ttcgatgctc catctatttc agcactttcg tatatctctt tagaaatccc
    26041 ttgaagacca gctaaaaata aaatagtgtt atatcctaca ctaagccaaa tcatcgtgat
    26101 gatcattgat attcgtatcc aaaaaggatt tgttaaccac tgtacaccgt taattcctag
    26161 cgcattcaaa aatccattga ataacccagt cggattgaaa aaagtcatga atacggaagc
    26221 tacagccacc gttgatgtaa tggttggaat ataatagaca gttctaaaaa atccttttcc
    26281 ttttaactgt gctgaattca ataaaacagc aaagtaaata gctaatagca tcgttattgg
    26341 aattgatcct gccatgataa tcaaaacatt cgtgtaagat ttaataaaca atggatctcg
    26401 gaagatagct agataattgt caaatccaac aaattcaaat gagtagccaa ttttcataaa
    26461 actaaaagca aacgagataa tgatgggaat caaaatcgtc acaataaaga aaataacaaa
    26521 aggtgtgacc aatccataag cacttttttt atgagacgta tctgttgctt ttttcttgct
    26581 gttctttatt gtcttgcttg gtaacgaaga ttgcatcatc gttaagtcct cctaatattt
    26641 tttaatcacc agttaatttt acgccgtact tatgagcaaa attttctgct tcttttagat
    26701 atgaattttc aaaatcacct gtttgccaat agttatatgc agcggtagtc aaataagacg
    26761 tagcatctga ataagtggct ggtaaaacag tatttgatgt attttcattg attttattca
    26821 atagttcata gtatttttgt cctccttagt agtcaaactt tttctcagct gcttctgttt
    26881 gataggccaa tgagttagat gtagccacgc ccagctccag tgcgttggct aagcattctt
    26941 catctgtcag cataaatgta agaaattgtt gtgccacttg tggatttttg gcatctttag
    27001 ggacataaaa acttgatcct cctgatactg caccttttcc tggattttct tttgaaaaag
    27061 gaatcagtgg agccatgcgc catttgtcag atgcatcagg aaaattcaaa gacatattcg
    27121 tagcatacca gccgccagca acaaacattg cagattcttg aaatgctttc tcagcatctt
    27181 gtgcaccgta gaaatgtaca atatctgagt cgatcatttc tttgattaac tttgcagcat
    27241 gtttcgcttc tttagtattc agacggatat ttccttcttc atcaagcaaa gattgattct
    27301 gctgggctag catgttgata aacaattctt ggtccccaga ttctggcaag gcaattccaa
    27361 aactaccagt tttttctttt aatgcttttg ccacctggat atattcttcc caagaactga
    27421 tttcttcaaa atctacacct atttcagcaa atttttttgg ttgataataa caaagtgctg
    27481 gggtaaaatc actagcccaa ggaataatcg tttcattact cgtttgttga cgcaaaatat
    27541 tcaatttttg atcataaaac tgatcgccat atttgtcata aaatccatat tcatcagcac
    27601 tataaaaagc atcttgaaat ttttccagca cacctgctgc tgaagtgtct gccatggaga
    27661 taatatccgg catatcttcg tgtgttacca attttggggt gatcatttgt actgttgctg
    27721 ttcccgaagc tacattcaca atttttaagt caatatttgc tccagttttg cggttataga
    27781 tttttgcagc attttcaaca tagtttgtca gtttgccgtt tgcgacatat acttctaatg
    27841 tagtggcttt ttttatcatt ggatctgcga tgtttgctgt tacttcttcg ttagtttttc
    27901 cacatgcact taagctgaat aatagggtaa tgtttagcaa agctatcacc aatttgtatc
    27961 tttttttcat gtctatccct tctttctttt caagttctta tataaaacgc tttcacttat
    28021 gtatattaac ggaattagta acac
//
LOCUS       DAZKCY010000026        27818 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000026 DAZKCY010000000
VERSION     DAZKCY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.026"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..1316
                     /locus_tag="ACG8XC_001029"
                     /pseudo
     CDS             189..1316
                     /locus_tag="ACG8XC_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            1414..1602
                     /locus_tag="ACG8XC_001030"
     CDS             1414..1602
                     /locus_tag="ACG8XC_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFP9190743.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1660..2013)
                     /locus_tag="ACG8XC_001031"
     CDS             complement(1660..2013)
                     /locus_tag="ACG8XC_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9190744.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2544..2966
                     /locus_tag="ACG8XC_001032"
     CDS             2544..2966
                     /locus_tag="ACG8XC_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9190745.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2963..3193
                     /locus_tag="ACG8XC_001033"
     CDS             2963..3193
                     /locus_tag="ACG8XC_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9190746.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3691..3891
                     /locus_tag="ACG8XC_001034"
     CDS             3691..3891
                     /locus_tag="ACG8XC_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFP9190747.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3918..5111
                     /locus_tag="ACG8XC_001035"
     CDS             3918..5111
                     /locus_tag="ACG8XC_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9190748.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5179..6744)
                     /gene="guaA"
                     /locus_tag="ACG8XC_001036"
     CDS             complement(5179..6744)
                     /gene="guaA"
                     /locus_tag="ACG8XC_001036"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9190749.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6803..7726)
                     /gene="coaA"
                     /locus_tag="ACG8XC_001037"
     CDS             complement(6803..7726)
                     /gene="coaA"
                     /locus_tag="ACG8XC_001037"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP9190750.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7922..8527
                     /locus_tag="ACG8XC_001038"
     CDS             7922..8527
                     /locus_tag="ACG8XC_001038"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9190751.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8835..9851
                     /locus_tag="ACG8XC_001039"
     CDS             8835..9851
                     /locus_tag="ACG8XC_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9190752.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9992..11293
                     /locus_tag="ACG8XC_001040"
     CDS             9992..11293
                     /locus_tag="ACG8XC_001040"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFP9190753.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11339..12001
                     /gene="deoC"
                     /locus_tag="ACG8XC_001041"
     CDS             11339..12001
                     /gene="deoC"
                     /locus_tag="ACG8XC_001041"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP9190754.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            12022..12420
                     /locus_tag="ACG8XC_001042"
     CDS             12022..12420
                     /locus_tag="ACG8XC_001042"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP9190755.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12486..13568
                     /locus_tag="ACG8XC_001043"
     CDS             12486..13568
                     /locus_tag="ACG8XC_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9190756.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13780..15342
                     /locus_tag="ACG8XC_001044"
     CDS             13780..15342
                     /locus_tag="ACG8XC_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190757.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15335..16468
                     /locus_tag="ACG8XC_001045"
     CDS             15335..16468
                     /locus_tag="ACG8XC_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9190758.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16468..17424
                     /locus_tag="ACG8XC_001046"
     CDS             16468..17424
                     /locus_tag="ACG8XC_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9190759.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17523..18689
                     /gene="deoB"
                     /locus_tag="ACG8XC_001047"
     CDS             17523..18689
                     /gene="deoB"
                     /locus_tag="ACG8XC_001047"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9190760.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18713..19531
                     /locus_tag="ACG8XC_001048"
     CDS             18713..19531
                     /locus_tag="ACG8XC_001048"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9190761.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19548..20255
                     /gene="deoD"
                     /locus_tag="ACG8XC_001049"
     CDS             19548..20255
                     /gene="deoD"
                     /locus_tag="ACG8XC_001049"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9190762.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(20553..21239)
                     /gene="gpmA"
                     /locus_tag="ACG8XC_001050"
     CDS             complement(20553..21239)
                     /gene="gpmA"
                     /locus_tag="ACG8XC_001050"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9190763.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(21411..21992)
                     /locus_tag="ACG8XC_001051"
     CDS             complement(21411..21992)
                     /locus_tag="ACG8XC_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9190764.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(22125..22808)
                     /gene="rpiA"
                     /locus_tag="ACG8XC_001052"
     CDS             complement(22125..22808)
                     /gene="rpiA"
                     /locus_tag="ACG8XC_001052"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP9190765.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            23127..23540
                     /gene="rpsL"
                     /locus_tag="ACG8XC_001053"
     CDS             23127..23540
                     /gene="rpsL"
                     /locus_tag="ACG8XC_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP9190766.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            23624..24094
                     /gene="rpsG"
                     /locus_tag="ACG8XC_001054"
     CDS             23624..24094
                     /gene="rpsG"
                     /locus_tag="ACG8XC_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP9190767.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            24188..26275
                     /gene="fusA"
                     /locus_tag="ACG8XC_001055"
     CDS             24188..26275
                     /gene="fusA"
                     /locus_tag="ACG8XC_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP9190768.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            26447..27634
                     /gene="tuf"
                     /locus_tag="ACG8XC_001056"
     CDS             26447..27634
                     /gene="tuf"
                     /locus_tag="ACG8XC_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9190769.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT         8794 a         5093 c         5868 g         8063 t
ORIGIN      
        1 aataagcaat tttttctata agttatatta tcataatacc acaaaatcac aaattttcat
       61 tcaattatca ttttcaattg cattttataa atcattttgt atgtaatttg gtatgtaagt
      121 taaaatgaaa aaagtagtcc attttgaagt acattttgtc attttgtact ccaaaactga
      181 actacttact tgcatatata cgcctttata gtggagtact acatttacga gattcggtta
      241 gagtatcaga aaaagaaaaa ataaaagtta cagaaatgta tacttcaata aatggtgaat
      301 tatgtaagat tgatagagct tattctggag aaattgttat tttgcaaaat gagtttttga
      361 agttaaatag tgttcttgga gatacaaaac tattgccaca gagaaaaaag attgaaaatc
      421 cgcaccctct actacaaaca actgttgaac cgagtaaacc tgaacagaga gaaatgttgc
      481 ttgatgccct tttggaaatc tcagatagtg atccgcttct acgatattac gtggattcta
      541 cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac
      601 tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc atttatatgg
      661 agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca aatcctttct
      721 gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg cagtatgaga
      781 gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg gaagggatac
      841 gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa atctgtttta
      901 agtacggttt atactatagc cctgttagta ctccagcaga ttttcggatg cttactccta
      961 ttgtactgga gcaagccttt agaaaagctg gaacagaatt gttagagcca tatcttagtt
     1021 ttaaagttta tgcaccacag gaatatcttt cacgggcata taacgatgct cccaaatatt
     1081 gtgcaaatat cgtaaatact caactgaaaa ataatgaggt cattattatt ggagaaattc
     1141 ctgctcgatg tattcaagat tatcgcaatg atttaacttt ttttacaaat gggcttagtg
     1201 tttgtttagc agagctaaaa ggatatcagg ttaccactgg cgaacctgtt tgccagaccc
     1261 gtcgtctaaa tagtcggata gataaagtaa gatatatgtt caataaaata acttagtgcg
     1321 ttttatgttg ttatataaat atggtttctt attaaataag atgaaatatt ctttaatata
     1381 gatttgaatt aaagtggaaa ggaggagatt gttattataa actacaagtg gatattgtgt
     1441 cctatttgtg gaaataaaac aagactacga atacgagtgg atactatact taaaaatttc
     1501 cctttatact gccccaaatg taagaacgaa actttaatta atgttcaaaa aatgaatata
     1561 ataacaatca aagagccaga cgccaagacg cagagccgat aatttgagaa atgaaactct
     1621 catcttatcg gctctttttg tttatctgaa ttttactgac tagccttcaa tatttcttgc
     1681 ttcattgata ccttttgcta gtgcttccat taaggatagt tctttgtctg taaagctatc
     1741 catgtatttt tctacctgta accttcgagt gcttttaatc aaattatctg taggtaagaa
     1801 aaattcatca atagatacat ggagtaatga tacaagatca tacagcactt gtgtacttgg
     1861 gtgttgtcct ttgttttcta tatttgttaa ataacgtggg tcaatttcaa tcattgttcc
     1921 cacttgttca cgagtgagac cccgtttcat tcgagcctct ttaatcgcta aaccaacggc
     1981 tctaaaatcg tatttatctt cttttttacg catactaaat cacctctata cattttactg
     2041 ttcctatata accataaaca ggtatagaaa aacatgttat atagtttatg agttcctata
     2101 gaacactgtg gaatttatta atgcgatgaa tccaatttca aatagaaagg tcgctgattt
     2161 tatttaccta tacaacttta taaaatttag tcatactcct agaaagtaga taaaattggc
     2221 gacttaatgg agggaaaagt cgccatgaga aagataatct gtcatctagt tctcttaatt
     2281 aagttcttat caaaaaaact tatccacctg tactggatag atatgaccat caatgtggtt
     2341 ttgcagatta catagataga aaaatcaaag catatgaaca gagaaaacaa tctgtttatg
     2401 tgcttttttg gtcacttaat aaatatcttt taggatttct cttcatttat gcaacaaatc
     2461 ccccgttcgt gtcggggttt agtatgaagg gagataaaga agcacatgcc actccctttc
     2521 acgacagaaa gggggtgaaa agcatgaaac catcttcttt tcaagaaacc atagaaaatc
     2581 agtttgacta tatctgcaaa caagttattg aagatgaacg aaaagattac ttcaaacatc
     2641 tagacagact atcagcaaaa gaagtctcgt tttctgatat agataactac cttgtcaact
     2701 ctttttctac tgttgatacg tacgtcactg atttccaact atttaaatta tttggattaa
     2761 gaatcaatgt tgaaagtgat ttattaagtg aagccctgcg tcatctttca gagaaaaaac
     2821 ggaacattat tttacttcat tactttatgg atatgagtga tgtggagatt gcggagttac
     2881 taaatttgaa tcgttcgact gtttatcgtc atcgaataag tggactagcc atgattaaag
     2941 aattcatgaa ggagtgtaaa gaatgaaaac agcttatcca agggttccct tccccctcat
     3001 tgtaaaggcg acagatggcg acgtggaagc aattaatcag attgttaaac attatcgagg
     3061 gtatacctca aaacgttcat tacgtcgcat gacagacgaa tatggaaatt ctcatatggt
     3121 tattgatgaa accctacgtg gcagaatgga aacgaaactc atctctaaga ttttatcgtt
     3181 cgaaattaaa tagtcccatt ttttcgtgga agcgtgtcga atcgtatcca cgcttcccga
     3241 tttgggaggt ttgttattcc atcaaagcat attgtgtgtt cagtaggttt tgataggcta
     3301 acaagccaaa cgttctttga caaccaaata aaggcaatcg aatacgtttc ggtaagaaaa
     3361 gagctgatga actaacacgc catgacctac aaaattagtg agcgattcat gttagtaatt
     3421 cgaaagtaat ctttagcagg attgctgcca tgactttctt atcgtgataa tgatactccc
     3481 gtatagccat agtccgagcg ttagaagcgt cacaggcaat gggtacggct acatgagaac
     3541 catgcagaag tgaaattcct gtgagctttg ctagagctgt tcgattgcta agatattctt
     3601 ctcttgtcag tgtattatta taaaactatt tgatataatt ataggtgaat attggagttt
     3661 tcctagccta taattgggaa aggagtaata atgataacgg tcacaaaaaa ggatttagtt
     3721 gagctaggat atggaacttc tttttcagca gacatcataa aaaaagcaaa agaattgatg
     3781 attacaaaag ggcatatcta ctatcaatct cgaaagctag atagagtccc tcgtgaagct
     3841 gttgaagaaa ttctaggaat tcggttttca aatgaaaaca ggtctgagta gtttttgcac
     3901 agtaaggagt aaaaactatg gttaaagacc caataaaaaa agcaaagaat ggaacctatt
     3961 attttcgtgc aaatctaggt tatgattcta aagggaaaaa aattcaaaaa tatcgtagtg
     4021 gattcactac taaaaaagaa gcaagagaag aatattcgaa actgctattg atgaaaccag
     4081 aagaattaag tgagaataag gataaaatga cgtttaaaca ttatatcttt gaaattttcc
     4141 ttccatggta taagacacag gtaaagctca gaacatatga aaatcgctta ccaactatta
     4201 agaagcattt ctcttatttt gataaaatgg ctgtttccga tattgagcct attgatgtgc
     4261 aaaattggca actaaaatta tcaaagaagt gtaagtcttc ttatgtaaga gctgttcaag
     4321 gactattttc agttgctatg gatcgagcaa ttgtccttgg gataaccacc acaaatccat
     4381 ctaagattat tggcaatgtg aaaaagcaaa agtctaaaat tgaattttgg acaaaagaag
     4441 aatttgaaaa agtaatctct ctaatttaca aagaagatta ctatcaacac tttttattta
     4501 tctctctgtg gtttttattt atgactggaa tgcgaattgg tgaagctact gccattcaat
     4561 gggaagatat tgattttgac tcaggagtac taaccatcaa caaaacactc tattacaaga
     4621 atcaatcgaa ttatagattt gttgaaccaa aaacaaaggc gagcgttcgt catattgtat
     4681 tagataaatg cacattaact tatttatccg aatggaagga agtacaacaa aatctaattc
     4741 agacagactt tgtaatgagc tataacggta ttcctaccca aaaacataca ctatcatatg
     4801 ctattgaacg ctatgctaag ctggctggta tccatcgaat cagaatccat gggttacggc
     4861 attctcatgc ttccttgtta atcagtatgg gagaaaatcc acttatcatt aaagatcgtc
     4921 taggacacga agatattgag acgactttag gaacgtatgg acatctttat cctaacagta
     4981 atttcgaggt agctaacaaa ttaaatggag ccatttcctt taaagaagca aaacaaaatt
     5041 atgatacttc accaaagaat caatttactg tagactactt gcgaaacaaa gaaatgaaaa
     5101 aagtgcaata aaagtgcagt aaaaagataa gaagagccag aaacccttgg tataagagga
     5161 tttctagctc tcctacaact attcccactc cactgttgct ggtggtttag aagtgatgtc
     5221 atacacgata cggttgacgt gggctacttc gtttacgata cgtacagaga ttttttgcaa
     5281 gacttcccat gggatacggg caaagtcagc ggtcattccg tcgattgaag ttacagcacg
     5341 gataccgact gtgtagtcgt aagtacgtcc gtcacccata acaccgacag agcggatccc
     5401 tggaagaaca gtgaaatatt gccagatatc acggtcaagt ccagcagctg cgatttcttc
     5461 acgcaagata gcatcagatt cgcgtacgat ttctagcttg tcttcagtga tttcgccaag
     5521 aacacgaata ccaagaccag gaccagggaa tggctgacgc caaacgattg aatcaggcat
     5581 gcctaactga gtccctaatt cacggacttc gtctttgaat aatgtgttta atggttcgat
     5641 caattcaaat tgcatatctt ctggcagtcc acctacatta tggtgagatt tgatcgtctg
     5701 tgctgtttct gtaccagatt cgataacgtc agtatacaac gttccttgtg ctaggaaaga
     5761 gacgccttct tcaccagcaa gttttgttgc ttcgtcatca aaaacatata cgaattcatt
     5821 cccaatgatt ttacgttttt gttctgggtc agatacacct ttcaacttgt ctaagaagcg
     5881 atctcttgca tcgactttga tgatgttcaa gccgaatttt ccgcctagac tttccattac
     5941 ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca aagatagatg tcaattggtc
     6001 accgatcgct ttttgtaaaa gaaccccaac gacactagaa tcaacgccac ctgaaagacc
     6061 aagcaagact tttttgtcac caacttgttc acggattttt tctacttgca tatcgataaa
     6121 gttatccatt gtccagttac cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa
     6181 ttcgattccg tattcggaat gacgtacttc tgcatggaat tggataccat aaaaattacg
     6241 tgttgtgtct tggatagaag caatcggaca atctttgctt gttccgactg tttcaaagcc
     6301 ttcaggaact ttagttacca agtcaccatg gctcatccat actgtttgtt gttttggtgt
     6361 ttctttaaac aagacagctt gatcgcttgt tacttctaac aaggctttcc catattcgcg
     6421 gtttttagca ggctctacgg aaccgccaag tttgtacgtg atcaattgca taccatagca
     6481 aatccctaat acaggaatac ctaaattgaa gatctcagga tcgatcccaa atgcattctc
     6541 atcatataca ctgtttggtc cgccagaaag aatgattccc tttggattca tcgcttttac
     6601 ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag actccaagat cacgaatacg
     6661 tcttgtgatc aattggttat actgactacc gtagtctaat acgataatct tttcaacgtt
     6721 cgtcatatcg gcaacgtttg tcaaatttat tacccctatc cctttaaatt ttatctcaaa
     6781 atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat
     6841 tttcagtttt atggagaatt atatcggctc ttcctcgtgt aggcaagata tattcctgta
     6901 aatttttcaa attcacattt ttccatacat cttttgccat tttaaaggct tcttcacgat
     6961 ctccgatagc aaaacgataa taataattct ctggattttg gaaagccgta tccagtaacg
     7021 caccaaaacg atccaaatac catttttcga tcagcacggg atcggcatcg acatagacag
     7081 aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc aggtaattga agcgtattga
     7141 tcccctcaac gatcaagata tcaggctgct ggatcaattc gtacttgtct ggaatgatat
     7201 catacacatc atgtgagtaa acaggtactt ttatctcttc ctgaccagat tttacacggt
     7261 tcaagaaatc gatcagcatc tccatatcat aactttcagg aaatccttta cggtccatga
     7321 ttccacgctc ttttaatata cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa
     7381 cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag cagtctggct gttgtacttt
     7441 ttccaacggc tacacttccg gcgatcccga taataaaagg cggtacttct acgtatttat
     7501 gaagaaataa acctttactc aagaacaaag attcaaattc tttcatataa agataaatca
     7561 gatgagcaag aggtacatag atatctcgaa catcctgcat ggaaatgcgg tcattcaaac
     7621 ttttgatatt atctagttca tcttgtgtca aaggtgcttg cccattacga taaaattctc
     7681 gccattcttc tcttgccaca cggtagtagt tcattggatc attcatatta gcctcctgcg
     7741 ttctcccatc aatggaacta ttttaacaca aaataaacgc aaaccccacc acttaagaga
     7801 gattgccgat ctttttgtat aatctttata atagaaagaa acggagtctt attcttgact
     7861 tgcgcacgga tacataaaag gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga
     7921 catgacaaat cattactatt ctgaacgccc agatacggcg cacgattttg aacaatgggc
     7981 ttttgagtta aaaggcagaa cctttcaatt tgtaacagat tctggtgtat tttcacgtga
     8041 aacagtggat tatggttcac gtgtcctgat tgatgctttc gattgggaag agttacctga
     8101 tgaaggaaag atcttagatg tgggatgcgg gtatggaccg attgggttag ctttggcatt
     8161 tgcgacacag cggtttgttg aaatggtcga cattaatgaa cgagcggtaa gtctagcgca
     8221 aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc catcaatcga atatctatga
     8281 agcagttcat gaagaaacct atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa
     8341 aaaagttgta catgaaattt tgacaggcgc atatcctcgg ttgaagaagg gcggtacact
     8401 gacaattgtg atccagaaga aacaaggagc gccgagtgcg caaaagaaaa tggaagaaac
     8461 atttggtaat gcagaaatcg tcacaaaaga taaaggatat tatatcatca aaagtgtgaa
     8521 agaataacta gctgaaaaat agtggagcga cgaaagagtg gaactgggcg tgcatgtacg
     8581 ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa acaaagaact attttttctt
     8641 caaaaagaaa tagtaggaac agagtcgttg ttttttacga taaaataaat aagatacaga
     8701 acttgtatag aaggctgcca agtcggtaga taaaggaggt ttttatcaaa aaacatactt
     8761 tccttttttc tctattcttt tcttgggggt tttggtacac tgatagggaa tttagataga
     8821 agggagccaa taggatgaag ctgaccatca gagatatcgc agaaatggca ggcgtatcag
     8881 taacaactgt ctcacagatt ttgaataaca aaggaagcag atttagtgat aagacaagaa
     8941 aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc cgattatttc gcttcaaatg
     9001 tgatcaatcg acattcaaag accatcggaa tgatcgtccc tgatgtcact gatttcttct
     9061 tctccaaact gatcgaaggt gtagagagct atttgaatcc tttgggctat gtgatcatgt
     9121 tatgcaattc tcgtcatagc caagaaaatg agatcaagta tttacaagag ctttcccacc
     9181 gctcagtgga cggaatcttg ctggccacac caaatatttt acctgagaaa tatgcattag
     9241 acagcggttt ttatcaaaaa atgccaatca ttttaatcga tcgcggattg aatcgtcggg
     9301 ataacggacg gcttatcgta aaggaatatg agggcgccta tcaagctgtt tcgctgttta
     9361 ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac cactggatac tatcaattag
     9421 aagaacgttt caacggatat cgtcatgcgc tgaaagatca tggcctgcct tttaatggga
     9481 aatttgttga acatggagag ttgactgtcc aaggtggata tactgcatca aaaaaattac
     9541 tcaaacataa agatatcacg gctatttttt gcgggaatga tgccatggcg atcggatgct
     9601 atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc ataggattcg
     9661 atggattaaa gctgtcagaa tacatgattc cgcaattgac gacagtgcag cagccaagtt
     9721 ttgatattgg attttatgct gcacgtttcc tgatcgatac gatcgaattt cctcaaagaa
     9781 aagttccgaa caaagtgttc gaaacaaaat taattattcg tgagagcgta aaagtattga
     9841 caaagggcta actcggcggt atattgctta tgtaagcatt tacaaaggct agttagtgct
     9901 ttttttattt ttagtaaacc agtttactaa agtggtttac ctttcctaaa aataatgtat
     9961 aataccgaat gaagacaagg aggatcaggc aatgcgaatg gtggatctga ttgaaaaaaa
    10021 gagggatgga aacgagctgt cgaaagagga aatcgagtac atcgtcacga actatacgaa
    10081 tggaaagatt ccagattatc aagtcagtgc attattgatg gcaatttttt atcaagacat
    10141 gacaaatgaa gaaatcacga acttaacatt ggcgattgct aattctggtg atgtcatcga
    10201 tttgagttct cttgaaggaa taaaggtaga taagcattcg acaggtggcg ttggtgacac
    10261 gaccacattg attttagccc ccctagtagc tagtgtcgga gtgacagtgg ctaaaatgtc
    10321 tggacgcggt ctaggttaca caggtggtac actagacaag ctagaagcaa ttcccggatt
    10381 tcagatcgag ctttcggatg aagaatttgt acgcattgtt aatgaaagta aagtagcggt
    10441 aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct tgcgtgacgt
    10501 cacagcgacc gttgattctc ttccgctgat tgccagttcg attatgagta aaaaaatcgc
    10561 ggcaggagca gatgcaatcg ttttggatgt gaccactgga gacggtgctt tcatgaagaa
    10621 catcgaagat gcaagacgtc tggcaaagac catgacgagc attgggaaac tggctaaccg
    10681 agaaacagtg gcggttatct cagatatgtc agaacctctt ggcgaagcta tcggaaatag
    10741 tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag atctagtaga
    10801 aatgtgctac gccttaggaa gccaaatggt tgttcttgct ggcaaagcta agacaaccga
    10861 tgaagcaaga acgttgcttc aagaagctct ggaatcaggt aaagcgttag caaaattcaa
    10921 agaaatgatc caaaatcaag ggggagaccc agcgatcgtg gaacatccag aacgaatttt
    10981 gactgcacgc tacacaatgg agcttcctgc taaacaaagc ggagttgtgt caaaaatcgt
    11041 ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa ccaaagaaga
    11101 agatattgat cacgctgttg gattgaaact gcataaaaaa attggtgaca ctgtgacaaa
    11161 aggtgagtca ctattgacga tctatagtaa cgacgaagaa atcacttctg tcattgaatt
    11221 attatataaa aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga ttcatgacat
    11281 catcacagaa taaaggaaaa aagcggactt taccgtttta aagaaaaggg gaaaagaaat
    11341 ggaactaaac cgaatgatcg accatactat tttgaaagca gacgcatcta aagaagctgt
    11401 gatgcaaatt atcgaagaag caaaaaaata ccatttttat tctgtctgca tcaatccagc
    11461 atgggttgcg ctagcgaaag aacaattaca aggtgagcca gtagccgtat gtacagtaat
    11521 cgggtttcct ctaggagcaa acacacccga gacaaaagca tttgaaacaa cagatgccat
    11581 caataacggt gcagatgaag tcgatatggt catcaatatc ggtgctttga aatcaaagga
    11641 tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag atcgtgcgct
    11701 tgtcaaagtt attattgaaa catcgttatt agatagagaa gaaatcatcc gagcttgcga
    11761 aatagcaaaa gctgcaggcg ctgattttgt caaaacatca actggtttct caacaggcgg
    11821 agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa tgggtgtcaa
    11881 agcttccggc ggtatccata acgaagaaga agcaatggca atgatcgaag ctggtgctac
    11941 aagaatcgga acaagcgcag gagtagcaat cgtatctggt tcaactggtg aaggctatta
    12001 attttttcaa aggagaagct catggcagaa ctcaaacaag aatggatcaa cacagctatt
    12061 gaagcgttag ataaagccta tgttccttat tcccattttc cggtaggcgc ttgtctcgta
    12121 acggaatcag gcaagattta tcaaggcatc aacattgaga atgcatcatt tggattaacg
    12181 aattgtgccg agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa aacatttacg
    12241 catctagtcg tagcggggca cacccccgat cctatctctc cttgtggtgc atgtcgacaa
    12301 gtaatggcag aattctgttc ccctgatatg ccggttacgc tagtaggaga caatggtgtg
    12361 acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa agatctataa
    12421 ctcatgttca ttcagtcagc tgactgttga aaaataaaac aattatttta ggaggcttta
    12481 cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt cgcactagca gcagggttat
    12541 ttttaggtgc atgcggcaac aacggctcta ctgattcatc cggtggcaaa agctcatcag
    12601 acacaacaac tgcagcattg atcacagaca caggtggtgt agacgaccgt tcattcaacc
    12661 agtcagcttg ggaaggacta gaaaaatggg ggaaagatca aggactttca cgaggaaatg
    12721 atggcttcca atatttccaa tcttcaaatg aatcagatta catcccaaat attgaccaag
    12781 cattgaatgc tggattcaaa acgatttttg gtattggtta taaactaaaa ccagcgatcc
    12841 aagaacaagc aacaaacaat acaggtacga actttgttat tatcgatgat gtcatcgatg
    12901 gattagataa cgttgtatct gcaactttca aagacaacga agcttcttat cttgcaggtg
    12961 ttgcagcagc ttatacgact gaaacaaacg tagtcggctt tatcggcggt gtaaaaggtg
    13021 aagtaatcga tcgttttgat gctggattca aagccggtgt tgatgcaggt gcgaaagaat
    13081 taggaaaaga aatcaaagta ttgaatcaat atgctggtga cttctctgct ccagataaag
    13141 gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc catgcttcag
    13201 gcggtacagg taacggtgta ttccaagaag caaaatcatt gaacgaatca ggcgacaaaa
    13261 aagtttgggt tatcggtgtt gaccgcgacc aatcagacga aggcgaatac acattaaacg
    13321 gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc agttggaaca gtagtagaag
    13381 atttggcaca aaaatcagcg gacggtaaat tccctggcgg cgaacacact gtttatggat
    13441 tgaaagaaga cggtgttggt ctaacagaag gacaactttc tgacgaagcg aaaaaagcag
    13501 ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa actcctgaag
    13561 aaaactaaaa ccatttctat agccttgtta gacacatgtt tgacaaggct atggtttgat
    13621 tagaacaaga attgttcatt tatccaagct gccttgtgac agtttggata agggaacaac
    13681 tccctgaagt aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat cttagtcaat
    13741 cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga aaatattgtc
    13801 atagaaatgc agaatattac caaagaattt ggaacattca aggcaaatga caacatcaat
    13861 ttacaagtaa aggcaggaga aattcatgcg ttgcttggag aaaacggtgc tggcaaatct
    13921 acattgatga acgtgctttc cggattatta gagccgacat cagggaaaat tttgatgcgt
    13981 gggaaagaag taaagatcac aagtccgacc aaagccaacc aattagggat tgggatggtc
    14041 catcagcact ttatgcttgt tgatgccttt actgtaacag aaaacatcgt gttgggaagc
    14101 gagcctagtc gcgcagggat gcttgaccat aaaaaagcac gaaaagagat ccaaaaagtt
    14161 tctgaacagt atggattgtc agtcaaccca gatgcttacg ttcgtgatat ttcagttgga
    14221 atggaacaac gggtagaaat tttaaaaaca ctttaccgag gagcagatgt actgattttt
    14281 gatgagccga cagctgtatt gacccctcaa gaaattgatg aattaatcgt gatcatgaag
    14341 gaattagtca aagaaggcaa gtcgatcatt ttgattacgc ataagttaga tgaaatcaaa
    14401 gcagtagctg accgctgtac agttatccgc cgtggaaaag gaatcggtac agtcaacgtt
    14461 aaagacgtaa cctcacaaca attagccgat atgatggttg gaagaacggt ttcattcaaa
    14521 acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa tctagtggtg
    14581 aaagaaaatc gtggattaga agccgtgaaa aacttgaact tagaggttcg tgctggcgaa
    14641 gtacttggta tcgctggaat cgatggaaac gggcagtcgg agttgatcca agctttgact
    14701 ggtttgcgaa aggcagaaag cggacatatc aagctaaaag gggaagacat cactaataaa
    14761 aaaccccgaa aaatcactga acatggtgta ggacacgtgc cagaagaccg tcataaatac
    14821 gggttagtcc tagatatgac attatctgaa aatatcgcac tacaaaccta tcatcaaaaa
    14881 ccttacagta aaaacggcat gctgaattat tcagtaatga atgaacatgc cagagaattg
    14941 atcgaagaat atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc tttatcaggc
    15001 ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga tctgttgatt
    15061 gttgcaaatc caactcgtgg actggatgta ggtgcgatcg aatttattca taaacgtctg
    15121 atcgaacaaa gggacaaata caaagcagtg ttactgatca gtttcgaatt agaagaaatt
    15181 ttaaatgttt cggatcgtat tgctgttatc catgaaggag aaattgtcgg tatcgttgat
    15241 ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg cgggctatac cttagaagaa
    15301 gcacgaaaag agttagaaaa gggggctggc gcacatgaat gatcgcagcg aaaaattccg
    15361 taatttatta gtgcctgtac tttcagttgt tttaggattg atcttaggcg cgatattgat
    15421 ggctgctttt ggttttcagc ccatccaagg ttattcttct atgctagatg cagcccttgg
    15481 ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg ccgttgattt ttacagcatt
    15541 aggtttttct gtagctaact cagctgggtt tttcaatatt gggctttctg gacaagcact
    15601 ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt ccagatctgc caaaagttgt
    15661 tttgcttccg ttatgcgtga tcttaggtgc cttagctggt gcgatggcag cggctgttcc
    15721 aggtttactt cgagcatttt ttggaacaag tgaagtgatc gtaacaatca tgatgaacta
    15781 tatcttatta tacgtaagca catttgcttt acatgaagtg atgcctgaaa gctatcgctc
    15841 aagtttggac tcttccaata tgatcagtca aaatgcctca ctgcggttac catggttaac
    15901 acaagctttt ggcggctctc gcgtaaactc aggattattt ttagcaatca tcgcgcttat
    15961 tttcgtttgg ctgctgatga agaaaacgac tttaggattt gaaatccgtt ctgttggatt
    16021 gaatcctttt gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg ttttgtcgat
    16081 ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt gaaggattag gaacgtatca
    16141 aaacttcttt gttcaaacga cttcgctatc tattggattt gacggaatgg ctgtttcctt
    16201 gcttggttct ggatcagcaa tcggtattct tttatcagcg atcttgttca gtattttgaa
    16261 gatcggtgga cttggcatgc agacaggagc aggtgttccg tttgagatcg tcaatgtatc
    16321 gattgctttg attatcttct ttgtcgggat caacttcctg attcgtttcg tcatggctaa
    16381 agtcttccaa gggaaaaaac aagaagagat cgttgcgaca atagaatcga aaccaacgaa
    16441 tcaaaaccat gaagggggcg aactataatg tcgactatcg aattaattgc cttgatcctt
    16501 acttctacac tggtttattc aacaccgcta attctaacgt cactaggcgg aactttttct
    16561 gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca tggtcatggg tgcttttagt
    16621 tccatcgttt tcaacttgac gatgagtcca tcacttggtt ctatgacacc atggatcgct
    16681 attttagtgg gtggcgtcgt aggtatccta ttttctctga ttcacgcagt agcaacagtc
    16741 agtttgcgtg ctgaccatat tatcagcggg acagtaatta atctgatggc acctgcttta
    16801 ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa ctgataacat cacgcagaat
    16861 ctggggtatt tttctttccc aggattagca gatattccgg tcattggacc aattttcttt
    16921 aaaaatacat tcttacctgc ttattgtgct attttgattg ctgtcctagc ttgggtgatc
    16981 atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc tcaagctgcc
    17041 gacacattag gtatcaatgt atatgctatg cgttattcag gcgtactgat ttctggcttg
    17101 ttaggcggta tgggcggtgc tgtttttgct cagtcgattt ccggtaactt ttcagctggt
    17161 acgatcgttg gacaagggtt catctccatg gctgctatga tctttggtaa gtggaatcct
    17221 ttaggggcaa tgggtgcctc attgttcttt ggttttgctc aaagcttgag tatcattggc
    17281 gcacaattac cagttatttc ttcgattccg ccagtattgc tgcaaattgc tccatatttg
    17341 ctgacaatca ttgtgctggt tgttttctta gggaaagctt ctggtccaaa agcaaacggg
    17401 aagaactata tcaaatcaaa ataatctttt agcgaataaa aagggtgggt tacgggaagt
    17461 gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa gaaggaataa
    17521 tcatgtttaa acgcgtacat ctgatcgtca tggattcagt aggaattgga gaagcaccag
    17581 atgccgataa gttcggtgat gttggttctg atacattagg acacattgcg aaagaagcag
    17641 gattgaacgt tccacattta gagcagcttg gtttaggaac gatccgtcct cttgaaggtg
    17701 tggaatcagt gaaagatcat caaggctatg cgacgaaatt agaagaagtt tctgtaggga
    17761 aagatacgat gacaggccac tgggaaatca tgggattgaa tatcaaaact ccatttcgtg
    17821 tgtttcctaa tggattccct gatgatctga tcaaacaaat cgaagaattt tctggccgta
    17881 aagtcgtatg taacaaacct tatagcggaa cagctgttat tgacgattac ggtgaacacc
    17941 aaatgaaaac aggcgattta attgtatata cttctgccga tcctgtcctt cagatcgcgg
    18001 cacatgaaga aatcattcct ttagaagaat tatatcggat ttgccaattc gttcgtgata
    18061 taacaaaaga tgatccgtat atgattggcc gaatcattgc acgtccgtac gtgggtgaac
    18121 caggagactt cacacgaaca agcaatcgcc atgattatgc gttgaatcca ttcggacaca
    18181 cagtccttga ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga aagatcaacg
    18241 atattttcaa cggacaagga atcactgaat ctgtccgcac gaaaagcaat atggatggcg
    18301 ttgaccagct gctgaaagtt atgaaaaacg atttttcagg aatcagcttt accaatttag
    18361 tagattttga tgcattatac ggtcatcgtc gggatgtagt cggctatgcg catgcgattg
    18421 aagaatttga cttacgaatt ccagaattgc ttgaagcgat ggaagaagac gacttgctaa
    18481 tgattacagc cgatcacgga aacgatccga cgtttactgg taccgatcat acgcgagaat
    18541 atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca aggggcattg cctcaaggat
    18601 attattcaga tatttcagca acgattgctg agaactttag tgtttcttcg actgaaaatg
    18661 gcgaaagctt cttgaaatta ttaaaataat ctttttaaat aggagaaagc aaatgaccaa
    18721 gttaagcgag atgttacaag aaacgaccca gtttatcctt gataaaggag taaaagaaat
    18781 tgattttggt ttgattctag gttcaggatt aggtgaatta gcagatgaaa tcgaagatgc
    18841 tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt tcgacagtag ttgggcatgc
    18901 aggaaagcta gtttatgggt ctctggcagg caagaaagtg ttagccatgc aagggagatt
    18961 ccactactat gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg tgatggccgg
    19021 actaggggcc cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg aatcatttgt
    19081 accaggagat ttgatgctga ttaccgatca tatcaatttt actggtgaaa atcctttgat
    19141 cggtccgaat gaagatgcaa tgggccctag atttccagat atgagtcaag cttatacgcc
    19201 tgactatcaa gcaacagcaa aagcagcagc agaaaaacta ggtttggatc ttaaagaagg
    19261 cgtatatatg ggctattctg gaccaactta tgaaacgcct gcggagatcc gaatgtctcg
    19321 gacaatagga gccgatgcag tagggatgtc gactgttcca gaagtcatcg tagcagctca
    19381 cagcggatta aaagtgttag gaatctcttg tattaccaac ttagcagcag gtatgcaagc
    19441 gaatctgagc catgaagaag ttgtagaaac aactcagaga gtcaaacaat cttttaaagc
    19501 attggttaaa gaaacattga cattattgta ataggaggaa caataaaatg agtgtgcata
    19561 ttgaagcgaa agaaggcgag atcgccgata agattttact tccgggagat cctttgcgag
    19621 ctaaatatat tgcggagact tttttggaag atccagtttg ttacaatcag gtacgcggta
    19681 tgttaggctt taccggcaaa tataaaggag aacgtatttc tgttcaagga acaggaatgg
    19741 gaatgccatc agccaccatc tatgcccatg aattgatcca gtcgtatggt gtaaagaaat
    19801 tgattcgagt aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt gatttagtac
    19861 tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa gcctttcatt
    19921 ttcctccaat cagtgatttc aatctattac tgaaagcata tgagatagct aaagaaaagg
    19981 gctatacggt tcatgtagga aatgttcttt cggaagattc tttctacaaa gatgatttga
    20041 cagagacgtt ccaattagct gaacttggcg ttttaggagt ggaaatggaa gcagcggctc
    20101 tttattactt aggcgcgaaa taccatgtgc agacattaag tttgatgacc gttagcgacc
    20161 atttgatcac cggtgaagaa acaacagctg cagaacgtca atctacgttc aatgaaatga
    20221 tcgaagtagg attggaaaca gcgatagcag aataagaagg aaatgaaaaa gctgccctgc
    20281 attaagtaat aagaacatcc aagcaaaaat agctcctcat gtttttgact tggctggact
    20341 tattattgca aatgtaagct tttgaacacc gtttaccaag aggttgtgaa tctgccgttt
    20401 aactctgagt cacaaggaac aatttcaaaa aacagctttt catgtttgat gaatattgtg
    20461 acttgtgacc gagaagttgg caggagaaaa ccgtttatac gaaaaaaggg actgtgacaa
    20521 atcgtgtcac agtccctttt ttatagtgtt ttttataggt agtatttttt agcaactgtt
    20581 aagtcatcat ttaattcata aacaagtggt tgacctgttg gaatttcaag atccatgatg
    20641 tcttcgtcag agataccttc gatatgttta gctagggcgc gtaatgagtt accatgtgca
    20701 gctactaaaa cagttttgtt gtctagtaat gctggagcaa tttcatcttg ccagaatggc
    20761 aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag gaatatcgcg tttgtctaac
    20821 attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag tttcttccat taatggagga
    20881 agagtatcgt aagaacgacg ccagatgtgc acttgttcgt cgccgtattt ttcagctgtt
    20941 tcttttttgt tcaaaccttg taatttacca tagtgacgtt cgtttaagcg ccatgatttg
    21001 atttgtggta cccaaagttg atctgaatat tctaagatca agttacatgt tttgattgca
    21061 cgagttaaaa cagaagtgta agcaacatca aattcgattc cagcttcttt gattttgcgt
    21121 ccgccttctt ttgcttcttc gattccttct ggtgctagat ttacatcagc ccaaccagta
    21181 aattggttca atgcattcca ttcactaaga ccgtgacgag aaaaaactaa ttttggcatg
    21241 tttagctctc cttttaagat aggtttatat ttctgaaatt ttcagttctg ttttattgta
    21301 caattttttg gaagagattt ccatcgaaaa attagataag attctatgag aaaacaagta
    21361 gagaatataa aaaaacgaac agtgcccacg caactgttcg tttttaggta ttagatgcat
    21421 aattgctcga tttctttttc aggtaaattg atatatttat aacatgttcc aactgaaaca
    21481 ccgcattttg aagaaataaa ttggatcgtt tctttttttt cataatagag acgtcggatt
    21541 tttttgatcg tttttgcatc gatttttggt cttccgccga tttttccttt tttacgagct
    21601 tcatctaatc caactaatgt acgttctttg atcaatgcgc actccatgtt tgctaggttt
    21661 tccattaatt ggaaatagat ttttcccatt tcgcgcgtat cgattccttc atcaagacta
    21721 atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt ctgttaattg ccgcgtagat
    21781 ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc cttgttgcat gtcagcgatc
    21841 actgactcca aatgaacagc agtagaatca ttttgttcca cttcatatgt ctcatggaaa
    21901 atttcttgac aaccaaagtc agataattga tcaacctgag ttgttaaatc aacgtcattt
    21961 atggttgtgc gtgcataacc aataattttc atggtgattc ctttctagca gtgattttct
    22021 aactggatat aacagtaaaa atgtagcata aagtacgtca aaaagatagt tttttcgcaa
    22081 attttccgct gtcttttttt attggatgat caagcagttt ttctttattt acgacggatt
    22141 gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta ctttagaaac catgtttagg
    22201 aaaagcccat gttctactac cccaacaagt tcatctaaat aggtagctaa gctttctggc
    22261 tgttcgatga cttctagatg cagatcaatg atgtaatgac caccatctgt ggttaagact
    22321 tctccgtttt cgtctgtacg caagacgggt tggaatcttt tttcttcaaa aatatgaagt
    22381 agctgttggg agccataagg gatcacttca actgggagtg ggaacttccc aagtttatcg
    22441 actaatttag aagaatcaac gatccaaatc gtttcttttg agtacgtagc gactattttc
    22501 tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa aatcagcact gatttcatca
    22561 gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa tactctttaa cggtattcct
    22621 aatttttccg cttgttcttt tgtcgcattt gaagtagtaa ctcctgtgat acgtaaacct
    22681 tcttcacgca tacggcgacc aatctcttct accatataat aggcggttga accggtcccg
    22741 agacctacga tcatcccgtc ctttacgaat ttcgcggctt caatgccagc taattgtttt
    22801 aaattcatct ttgcgtccac ctttttttag tttgagcatc gtgctttcct cactatttta
    22861 gcgtgattta cggtaaaaag ataccattct tttttattcc aataaagctt tttcaaaaaa
    22921 aattgcatct cttgttgaca tgttttttca ttcgtgatat tctaacaaag gtgctttatc
    22981 tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa gcatatttga taaggatgca
    23041 gacattgcat ttatttttta tcacaaaaaa agaaccacct ggatgtgtgg aactagacgc
    23101 aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa ccaattagta cgtaaacctc
    23161 gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa caaaggatat aacagcttta
    23221 agaaaactca aactaacgta aactcacctc aaaaacgtgg ggtagcaact cgtgtgggta
    23281 caatgacacc taaaaaaccg aactcagctt tacgtaaata tgcacgtgtt cgtttgtcaa
    23341 acttaattga agttactgct tatatcccag ggattggtca taacttacaa gaacacagtg
    23401 ttgtattatt acgcggtggt cgtgtaaaag acttaccagg ggtacgttac catatcgttc
    23461 gcggtgcttt agatactgct ggtgttaacg accgtaaaca aagccgttct aaatacggaa
    23521 ctaaaaaacc taaagcttaa ttttaattat taaacaaatg aaacttgctt ctactatata
    23581 gaagtaatta ctatgaacaa tatttcggaa ggaggagtta cggatgccac gtaaaggtcc
    23641 tgttgcaaaa cgtgatgttt taccagatcc tatttataac tcaaaattag taactcgctt
    23701 gatcaaccgt gtaatggttg acggaaaacg cgggattgca gctaacatta tctataatgc
    23761 ctttgacttg atcaaagaat ctacaggtaa cgatccatta gaagtgtttg aacaagcaat
    23821 gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt gttgggggtt caaactacca
    23881 agtaccagtt gaagttcgcc ctgaacgccg cgttactcta ggtcttcgtt gggtagtaaa
    23941 ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc ttagcaaaag aaatcatgga
    24001 tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa gacactcata aaatggctga
    24061 tgccaaccgt gcgtttgctc actatcgctg gtaagattct ctctgtttgg tcggctttta
    24121 taagtcgacc tcagatagtg tccttatttt tagcaactag atagtaaaga gaggagaaac
    24181 aaggagaatg accagagaat tttctttaca aaacactcgt aatatcggta tcatggccca
    24241 catcgatgct ggtaaaacga ctgctactga acgtatcttg tactatactg gtaaaatcca
    24301 taaaattggt gaaactcatg aaggagcttc acaaatggac tggatggaac aagaacaaga
    24361 acgtggtatt acgatcactt ctgctgctac tacagcacaa tggaagggtc accgaatcaa
    24421 catcatcgac acaccaggac acgtagactt cacagttgaa gttgaacgtt cattgcgtgt
    24481 acttgatggt gctgtaactg ttcttgatgc acaatcaggt gttgaacctc aaactgaaac
    24541 agtttggcgt caagcaacaa cttatggtgt acctcgtatc gtatttgcaa acaaaatgga
    24601 caagatcggt gctgacttct tatattcagt atcaacttta catgaccgtt tgcaagcaaa
    24661 cgctcaccca atccaattac caattggtgc ggaagacgac ttcacaggaa ttatcgacct
    24721 tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga acagagatcg aagaaacaga
    24781 aattcctgaa gaataccgcg aattagctga agaatggcgc gaaaaattgg ttgaagctgt
    24841 agctgaaact gatgaagaac ttactttgaa atacttagaa ggtgaagaaa tcactgaagc
    24901 tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta gaattctacc cagtactatg
    24961 tggttcagca tttaagaaca aaggggttca attactattg gacgctgttc ttgattactt
    25021 gccatcaccg ttagatatcc cagctatcaa agggattgac cctaaaactg atgaagaagt
    25081 agaacgtcct gcagacgaca gcgctccttt ctcagcttta gcatttaaag ttatgactga
    25141 cccattcgta ggtcgtttaa ctttcttccg tgtgtactct ggtgttttac aatcaggttc
    25201 atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt ggtcgtatcc tacagatgca
    25261 tgccaactct cgttcagaaa tctctgaagt ttacgctgga gatatcgctg ctgctgttgg
    25321 tttgaaagac acaactactg gggacacact atgtgatgaa aagagtctag ttattcttga
    25381 atccatggaa ttcccagaac cagttatcca agtcgctatt gaacctaaat caaaagctga
    25441 ccaagataaa atgggtgtgg ctttacaaaa actatctgaa gaagatccta ctttccgtgc
    25501 tgaaacaaac gtggaaacag gcgaaacaat catcgctggt atgggtgaac ttcacttaga
    25561 tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa gctaacgtag gtgcgcctca
    25621 agtatcttac cgtgaaactt tccgtgctgg cactcaagcc gaaggtaagt ttgtacgtca
    25681 gtctggtggt aaaggtcaat acggtcacgt atggattgaa tttacaccaa atgaagaagg
    25741 tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt gttcctcgtg aatacattcc
    25801 agctgttgaa actggtttga aagatgctat ggaaaatggt gtcttagctg gctatccatt
    25861 agtagatatc aaagctaaac tttacgatgg ttcataccat gatgtcgatt caaatgaaac
    25921 tgccttccgt gttgctgctt ctatggcatt acgtgcagca gctaagaaag ctaatccagt
    25981 aatccttgaa ccaatcatgg ctgtagaagt tgttatccct gaagattatt taggtgatgt
    26041 tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt atggaagctc gcgctggcgg
    26101 ccaacaagtt gttcgtgcga tggttccact tgctgaaatg tttggttatg ctacaactct
    26161 tcgttctgct actcaaggtc gtggtacatt tacaatgaca tttgaccact acgaagatgt
    26221 accaaaatca gtacaagaag aaatcattaa gaaaaacggc ggaaaagctg aataatttct
    26281 taaaaccgga tagtcctgca aagagattat ccaaagagac tatattttta atatagtttc
    26341 gtgtaaaata attatcgatg atatgggcat gtggtgtacc ttgtgtacat catataccta
    26401 tcagacaaat aaaaaatata ttgtattttt aggaggacat tttaaaatgg caaaagaaaa
    26461 atttgaccgt tctaaaccac acgttaacat tggtactatc ggacacgttg accatggtaa
    26521 aactacatta acagctgcaa tcacaactgt actatctaag aaaaacggcg gccaagctat
    26581 ggcttacgat caaatcgatg gtgctccaga agaacgcgaa cgcggaatca caatctcaac
    26641 agctcacgtt gagtatgaaa cagatacacg ccactacgct cacgttgact gcccaggaca
    26701 cgcggattac gttaaaaaca tgatcactgg tgctgctcaa atggacggag ctatcttggt
    26761 agtttctgct gctgacggcc caatgcctca aactcgtgaa cacatcctat tgtctcgtca
    26821 agttggtgtt ccttacatcg ttgtattctt gaacaaagta gacatggttg atgacgaaga
    26881 attactagaa ttagttgaaa tggaagttcg tgacctatta acagaatacg aattccctgg
    26941 tgacgatgtt cctgtagttg ctggatcagc tttgaaagct ctagaaggcg acgcttcata
    27001 cgaagaaaaa attcttgaat taatggctgc agttgacgaa tacatcccaa ctccagaacg
    27061 tgacaacgac aaaccattca tgatgccagt tgaagacgtg ttctcaatta ctggacgtgg
    27121 tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc gttggtgacg aagttgaagt
    27181 tgttggtatt gctgaagaaa cttcaaaaac aacagttact ggtgttgaaa tgttccgtaa
    27241 attgttagac tacgctgaag ctggagacaa cattggtgct ttactacgtg gtgttgcacg
    27301 tgaagacatc caacgtggac aagttttagc taaaccaggt acaatcacac ctcatacaaa
    27361 attctctgca gaagtatacg tgttgacaaa agaagaaggt ggacgtcata ctccattctt
    27421 cactaactac cgtccacaat tctacttccg tacaactgac gtaacaggtg ttgttgaatt
    27481 accagaagga actgaaatgg ttatgcctgg cgacaacgta acaatggaag ttgaattaat
    27541 ccacccaatc gctatcgaaa acggtactaa attctcaatc cgtgaaggcg gacgtacagt
    27601 tggtgccggt gtcgttacag aaatcaaagc ttaattcact taagttttaa agtaaagcaa
    27661 catttattcg gacgtaatcg ctcggaacga aggtcttagg atctttgttc cgagcttttt
    27721 tatttattgc aggatcagga aaaaagacag gagtattata aatagaagaa acacgaaata
    27781 aaagagtcag tattttctaa atgaaacttt atgaaaac
//
LOCUS       DAZKCY010000027        27703 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000027 DAZKCY010000000
VERSION     DAZKCY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.027"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(267..1211)
                     /locus_tag="ACG8XC_001057"
     CDS             complement(267..1211)
                     /locus_tag="ACG8XC_001057"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP9190770.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1507..2451)
                     /locus_tag="ACG8XC_001058"
     CDS             complement(1507..2451)
                     /locus_tag="ACG8XC_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9190771.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2672..3886
                     /locus_tag="ACG8XC_001059"
     CDS             2672..3886
                     /locus_tag="ACG8XC_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9190772.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3908..5923
                     /locus_tag="ACG8XC_001060"
     CDS             3908..5923
                     /locus_tag="ACG8XC_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFP9190773.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6006..7379)
                     /locus_tag="ACG8XC_001061"
     CDS             complement(6006..7379)
                     /locus_tag="ACG8XC_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9190774.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7405..7689)
                     /locus_tag="ACG8XC_001062"
     CDS             complement(7405..7689)
                     /locus_tag="ACG8XC_001062"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9190775.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7691..9706)
                     /locus_tag="ACG8XC_001063"
     CDS             complement(7691..9706)
                     /locus_tag="ACG8XC_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9190776.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9885..10190)
                     /locus_tag="ACG8XC_001064"
     CDS             complement(9885..10190)
                     /locus_tag="ACG8XC_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFP9190777.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10279..11310)
                     /locus_tag="ACG8XC_001065"
     CDS             complement(10279..11310)
                     /locus_tag="ACG8XC_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9190778.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11517..12392
                     /locus_tag="ACG8XC_001066"
     CDS             11517..12392
                     /locus_tag="ACG8XC_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9190779.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12544..13386)
                     /locus_tag="ACG8XC_001067"
     CDS             complement(12544..13386)
                     /locus_tag="ACG8XC_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9190780.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13478..15355)
                     /locus_tag="ACG8XC_001068"
     CDS             complement(13478..15355)
                     /locus_tag="ACG8XC_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9190781.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15352..16773)
                     /locus_tag="ACG8XC_001069"
     CDS             complement(15352..16773)
                     /locus_tag="ACG8XC_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9190782.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16776..20165)
                     /locus_tag="ACG8XC_001070"
     CDS             complement(16776..20165)
                     /locus_tag="ACG8XC_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9190783.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20422..21813
                     /locus_tag="ACG8XC_001071"
     CDS             20422..21813
                     /locus_tag="ACG8XC_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9190784.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21886..22533)
                     /locus_tag="ACG8XC_001072"
     CDS             complement(21886..22533)
                     /locus_tag="ACG8XC_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9190785.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22541..23227)
                     /locus_tag="ACG8XC_001073"
     CDS             complement(22541..23227)
                     /locus_tag="ACG8XC_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190786.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23233..24306)
                     /locus_tag="ACG8XC_001074"
     CDS             complement(23233..24306)
                     /locus_tag="ACG8XC_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9190787.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24528..25202
                     /locus_tag="ACG8XC_001075"
     CDS             24528..25202
                     /locus_tag="ACG8XC_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9190788.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25207..26547
                     /locus_tag="ACG8XC_001076"
     CDS             25207..26547
                     /locus_tag="ACG8XC_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9190789.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26696..27682)
                     /locus_tag="ACG8XC_001077"
     CDS             complement(26696..27682)
                     /locus_tag="ACG8XC_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFP9190790.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8077 a         5242 c         5031 g         9353 t
ORIGIN      
        1 aaatatgaac gaattagata tgaatcattg tatttgccgt tgcggaagga ttttttaatt
       61 aaccacagat aaacattgat aacgattcgc ttattgtaat caacagatta aatttcatat
      121 gttcatatgt acggatacat tttcggaccg attaatttta accattctgt ttatttgctt
      181 acctattaga tatcaataaa aaactgctta acagctattt cagctgttaa gcagttttag
      241 gatttttagt tcatattcat tgtttcttat aatacagtgt tcaatacttc tcttaaagca
      301 cttgttgatt tgctgaacat tgctttttct tcttcgttga ttgataattc aacaacttca
      361 cgaacaccat tttcgtcaac gattgatggc acacctgtaa agatatcttt ttcgtcgtat
      421 tcaccagtta aataagcaga tacaggcaat acagcttgtt cgttattcaa gatagctttt
      481 acgatacgtg cagtactcat accaatacca tagtaagttg cttttttacg atcaatgatc
      541 tcataagcag cattacggac tttatcagca atcacatcta attcatcttg ggcaatacga
      601 tggtcttttt ctacaatttc aaacactggt ttaccaccga cagttgtatg tgaccatgca
      661 gcaacttctg aatcaccatg ttcacctaag atatagccgt ggacgctgcg tgggtcaact
      721 tttaatttca atgcgatttc tttacggaaa cgagttgtat ccaatgttgt tccagtaccg
      781 ataacacgtg atgttggcag accagattcg ttccaagcga tatatgtcaa aatatctact
      841 gggtttgaag caacaacgat aataccgtcg aatcctgatc ccatgatttc ttttacgatt
      901 tggcgcatga ttgacgcatt tgttttaact aaatctaaac gagattgacc aggtttttgg
      961 ttgataccag ctgtgataac aacgatatca gcatctttac attcttcata gccacctgac
     1021 catacagcca cattttcatc gccccaagcc ataccatcta aaaggtccaa tgcttcccct
     1081 tctgcttttt cttgattcac atcgatcaat actaattcat tagagattcc ttggttgatc
     1141 attgcataag cgatacttgt tccaacaaaa cctgtaccga cgattactac tttacgactt
     1201 gtttttttca tgaataaaca gctccttttc ataactattt gttactttca tgaaaagtat
     1261 agtcttgttt attcatttta tcaacatgtt aattataata gttgttatta taatttttga
     1321 tggaaacgaa ttcctgaata cgcataacag ataaatgaca aatgtttaca cctatatccc
     1381 ctgataatac aaccaaaagt ttagaactga aaataaatcc aaaaaaattc gctgcaacga
     1441 tataataaga cagcaatcgc acctaaaaca attttccttc atatgcttta gtgaaagatt
     1501 ttcttcttaa tgtttatttc tccattcatt gggagtacat gttgaatact tcttgaataa
     1561 tttgaataaa gaagtcgttt gtttatatcc gatacgatta gcgatttctt ccaacgaaaa
     1621 actcgtttct tgaatcagtt ccaacgcaaa gtcgaatttt ttctttagaa ccagttcttg
     1681 aaaggaagca tcaaaataca ttttcaattt attactaaga tagaccggat tgtagccaaa
     1741 atgagctgct gtttctttta acgtacaaga agtaaaatgc ttatcgatat aatctaatac
     1801 tctaagcatc tcattttctg cgcatccatc tgctgatgaa aagctttgtt gttctaataa
     1861 atgagtcaat tcgatcagta atagtgatgt atagagattt aacgaacgga tcttatgact
     1921 gtatgtatca aaataaatca acagcatatt ttcaatcaaa tgcgtcaaac gacttgtttt
     1981 tccgccgcga aaatgaatga attgtgtatg ttcccctttt tcttttgatg catttatcaa
     2041 aaaagaagaa agtagattat ttgtgcttgc caaatgattc aatatctctg tagataaggt
     2101 ttcttctttt aataagatgt tgattaaaat atcctcttgc cctgtgtatt cgattctttg
     2161 agtagcttct ttatcaatga tcaccacgtc actttcttcc atctctatcc tttctccatt
     2221 aataatttgt aaacatgtac cgctatacat ataattgatt tcaacaaaat catgtgtatg
     2281 ctcgggaact tccgcatatc gacggtgttt actgatatga atatttccct gttgaaatag
     2341 atcccctcgg tggatttttg gcggtgtctg aagatggtaa gctaagttta aatcattgat
     2401 attcattcct tgttcacgct gcagagtttc gattgattct tcgttacgca aaaaaaatgt
     2461 ctaattcagc tagttccaat tgactccctc ctttttgtaa gcggtttctt tcgcttatta
     2521 gttcacgctg tactgctatt atatgcaaag ctatttttgg tttcataaag actttctatg
     2581 gttgggacat gaacttcctg tgattccgct attgagagaa taaccacttc atactatatt
     2641 tttgataagc gcttacaaaa atgaaaggaa catgcttatg aataaaaaga tgatgaaatt
     2701 ttctctgctg tcagtctctc ttattttgac ttctgctggt gcagtcacgt ctgcaattcc
     2761 cagtatgtca gctacttatg ccacgcgttc tttatctgct gttgaattat taacgactgc
     2821 tccctctttt atggtcacta tttttgtttt gattagtagt tttattgcta ggaaaatcgg
     2881 acaaaaacaa actgtgatca tcggattagt gattgcatcg atttgtggta tcctccccct
     2941 cttctcacaa agttacagta ttgtcttagc ttcaagagct ggactaggta ttggctttgg
     3001 attgatcaat tctttggcag tcagcatgat tgctgccttt tatcacggag atgaacgagc
     3061 ctctatgatt ggttttcaaa gtgcatttca aggatttggt gcagctttaa tgacatttgt
     3121 tgcaggacag ctagtacagt tcggttggca atataccttt ttagtctacg ccatcagctt
     3181 acctattctt gtcttgtttg ttttgtttgt tcctacacct gaaacaactg aacttcaatc
     3241 gcatggcaca gccccaacaa gacaagtaat caaaacgaat cctaaaatga ttagttatgg
     3301 tatttttctc ttgctggtca tcgtggtata taatgctgta tcgattaaat tagccacagt
     3361 cctgacgact tattctctag gaaacgaaac taatgcagcg aatatcttaa caatcatgca
     3421 attggcaagt atggctgccg gagtttgttt tggctggctt cagacaaaag cacatcgcta
     3481 tatgcttaca ctttctttac tactgatggg aatcggcttt gtattgattg ccactgtgat
     3541 aaacctgtat gtgattgggt ttggtgcgat cctaacagga atttcttttt cgttattcat
     3601 tccttactta ttcaaccaag taagtatcca agcgcccaag tacgcggaga gtttcaatac
     3661 ttcaatctta ttggttggcg ctaatttagg tactttcatt gcaccatatg gtttgctctt
     3721 tctctctttt gcttctcttg ggagtaaaac acttgctgtt ttcgtcaatg gcagtatctt
     3781 gctgttgatt agcgcgctta ttagccttct agtagtacac aggacgaata gaaagacagt
     3841 gataagagta gatgaagaaa taaaagaaca attgacaaca aaataaattt gatggaggtc
     3901 gtgtaaaatg acaaatgaaa aacataaaga tagatggctt tggtatcctg gagactttga
     3961 gatccgccat ggactgctac aaaatttcca acgggaagaa cgaggttttg actggccagc
     4021 gtactggtac atggatgatt gccatcggaa cgtcaagttt aagagatatt actttcttga
     4081 tcaaccttcc atgtttaaag tgacgatcca aggagttggg tatgtagaaa ttaatggaca
     4141 aaaacatcca tgtggaaaat ggctgacttg tccagctggt aaagcaaaaa ttcgtatctt
     4201 tgtaggacat acatcagggc taccagcgat gtttatcgag ggagacgaag taaaatcaga
     4261 tattggctgg acagcaagta attttattga agaatatcca gctggctgga gtccgcttta
     4321 tgtcgatatc gcaaaagatc caaatcaaat ctattatcaa aaagaatgcg tccatccagt
     4381 cactgaaacg gagattggcg gaggaatctt attcgatttt gggcgagcag tgaatggctg
     4441 tgtagaacta atatctctgg atgaccatat cgaatctatc actctatgtt atggagagtc
     4501 cgatgtagaa gctatggata tcgattattg ctactataag caaataggaa tgacaagaaa
     4561 aatgaaagct cgtaaacgcg cttttcgcta tttgtttata ccagaggttt ctactggaat
     4621 ggttcaactt caagtttttc atgaattttt atctctttcg aaacaagctg ctttttcaag
     4681 cgattcttct ttactgaatc agatctggtc agtatcagaa gaagccttca agttatgtag
     4741 tgatttgttt tttatcgacg gtatcaaacg agatcgctgg atttggtctg gagatgcgta
     4801 tcaatcattc atgatcaatc aatatctttt ttttgacgag gaaatcaata aacgaacgat
     4861 attagcatta agaggacaaa atgaaatcaa gcagcatatg aatactatcg ttgactattc
     4921 gatcttgtgg ttgattggta tcgaaaacca ctatatgatg acgaaagact atgaattttt
     4981 acgcatcgtc taccctaaga tgcaatcatt aatgagatac ttgatggagc aaacgaatga
     5041 attagggttt atttatggga gagcaaaaga ttggatattt attgattggt ctgaaatgga
     5101 taaagaagga acgcttgctg cagaacaagt cttattactg aaagcctatc attccatgat
     5161 cacttgcgga gatatcttag ctgaaacata tggagagaac gaaaattatc atactcatct
     5221 ctatcaagaa aaatatcaac tattgaaaca aaacctattt gactactttt gggatgaaga
     5281 acttggcgct tttatcgatt gctatgaatc tgggagaaga aatgtgacgc gtcatgcgaa
     5341 tattttcgct gttttattcg attttgctaa caaagaccaa cagcaatcga tcttaaagaa
     5401 tgtcttattg aatgacacta tcacacagat tactactcca tatttcaaat tctatgaaca
     5461 agatgcttta tgcaaactcg gacagacgaa tatcgtctac caaacgattc ttgattattg
     5521 gggagggatg ttatctcatg atgcggtaac tttctgggaa gaatacgacc ctactcaaac
     5581 aggaaatgaa aagtacagta tgtatggaga tccttatgga aaaagcttat gccatgcatg
     5641 gggagcaagc ccaatctatc ttttaggacg ctattttatc ggtttgtatc caacaaagcc
     5701 aggttatgaa gaatttatga tacaacctaa tctagattca tttgaaacac tgaattgtac
     5761 gctacctatc aaagatggaa agatttcgct tgaaaaaaca aaagataaga tcgtgattac
     5821 ttctgataga acaggcggaa cagtgaaaat gaatggtaaa agttatcctc ttctagcaaa
     5881 acagccactt gtcttaacaa gctattcaaa aataaatgca taaaacaaaa aactgcttgc
     5941 gacatatcag aagactttaa atcttcttct gatattgtcc aagcaatttt tattttgaag
     6001 tttgcttaat tctgttcttt agaaacaatg tttgttcttt ttccggcaaa aagaaagcta
     6061 gataatacga caagtaaaaa gaaagcgatg attccaactg tcaaggctat ttgaccgtat
     6121 tctcctagtt tcccaagtac aagtcctggt actagataat cagtatcggc aaatgtagaa
     6181 gaagcgattt taagatctcc taatactgga aggataaata atggcagcca actaatcaat
     6241 aagctattga aagcagaacc taataccgcc cctcttctac cgccagtcga gttgccgaag
     6301 acaccagaag cagctccgca gaagaaatgt cctactaccc caggaatgat aactgtagtt
     6361 tgcaagccta tcatagcaaa catggatagt gttcctacaa agaagctaac aaaaaagcca
     6421 attaatacag cattaggtgc atatggaaaa acgataggaa catctaatgc tggtttagaa
     6481 tcgggtacga gttttttagc aatcccttgg aatgctggaa cgatttctgc caacaccatt
     6541 cgaacccctt gaaggacgat aacaaaccca gctgtgaatg ttccagcttg gatcagtgca
     6601 aagattacag cgttgtctcc attgcttagt tctctttcaa caaatccagg accagctaac
     6661 aaagcaagtg cgacatatac gatcgacatc aagagcatga tcgatacagt agaatctcgc
     6721 aaaaagctga aacttttagg aatctttatg tcctctgtag aacggtcttt gtttccaaaa
     6781 aactttccga tccatccact agaagcataa ccgatatttc cggtatgccc taacgcaact
     6841 tgattgtttc cagtgatgtt cttcataaac ggttgacaaa gagcaggcgt aatagttaat
     6901 agtgttcctt cgaagacccc gcctaataag atcatccaca aatttgcagt aaaccccgcg
     6961 cttactaaaa tgactgctgt tagacaagaa acataaagca ttgcttgacc tgtaagaaaa
     7021 atgaatttat atcttgtgaa tctagcaagt aaaatattca cgatcatccc tactagcatg
     7081 atcaaagcag taggtgtacc gtattgttgc aaagcaatcg ccactactgc ttcattgcta
     7141 ggaactactc cttgagtatg cagagctgtt tggaacatcg aagcaaaagg ggttagtgaa
     7201 ttactgatta aaccagcacc gccgatcagt actaggaatc cgacaaatgt tttgatagtt
     7261 ccttggatca cttctgaaaa tttcttcttc tgaagaacga gaccaagcag tgcaatcaaa
     7321 gaaacgagga tggagggctg actgacgatt gacacaaaca aattcaacaa tttatccatt
     7381 gatagttcct cccttgaatc atttttattc ttttgacaag atcttcgtta ctttttcttt
     7441 tacttcttcg ctgtcaatga tcgattgcaa aggtacgagc ttttcatcag gtatagaagt
     7501 tagcgtatct ttcaaagaat gctcaatgaa gaaataatca gccatatcgc tagatacact
     7561 tccaacatcc gtatgactta cttcaaactc gtccacatct gctccaagat ctgcaagtgc
     7621 ttgtttgatg ttcatctcta ccataaaact tgttcctaat ccactttgac atactgcaaa
     7681 aattttcata tcattcgtct ccttttctta tgatcgctaa tatttcttct tttgttgatg
     7741 cgtgtaataa atcttgaaga ttttgtttat tagaaagtag ctttgttaaa tttgccaaag
     7801 cccgaagatg tgcctcatta tcgatagcag ctaaacagat gaacaaacta atgggatggg
     7861 ctggattatc aagcagatcc acaggttgtt gaagtttcag caaagacatc cctacttcgt
     7921 ttacgccatc ttctggacga gcatgaggta atgcgatgtg atcgccaata tggataaacg
     7981 caccatactg ctccacgcgc tcgatcatag ctttgatata actttcttct attttatttt
     8041 ctgataatag cggctgtgcg gcaaatgtga ttgcttcttg ccagtttttt ggttgatccg
     8101 ataattggat catttcttct gttaataatt cagatagcat aggacgttga tcctccttct
     8161 tttctaaaag tttgttcatt ttacgattta atacttgata gagcttttct tcgtctacgc
     8221 cttcttttaa ttcgatataa ggcagtagcg ccgacaacaa ttcttgggca gaaggcattg
     8281 aaaatcctgg tatcagccaa tctctttgta cttgattgat cagctgattt ttttccaagt
     8341 cagtcatgat tggatgcaga acatagagag actttttctt atcagtaagt gtgagaggta
     8401 cagtagaaaa aataatatcg taagaagttt cgtcaacctc gtgtaagcgg tcagctgaat
     8461 tagtctctgt aaaaaagatc atcgggaata attgctgaag ttcacttttt aagatcaatg
     8521 aagagctaat gccacttgga cagacgatca cggctcttat ttttctattt ctttcttctg
     8581 cgtccgcttt tctgatctct ccgccaaata gtatagtgaa aaaaccaatc tcttcttcag
     8641 ggatgctctt ccctattcgt ctttctaacg gaagtaatgc ttttcttgtc atttcgtaga
     8701 tggaagcata ttgatgctta atttgatcga tcatcacatt aggtaaataa aaagaatagg
     8761 aaatacgaaa ataggcaggg accaagtgat aaaagacatt catcaataat tcacgaaacg
     8821 aagcaaattc tacggccatc aggaattcca tgcgatggat gacttctgaa gaacattcca
     8881 gcaaaaaatc aagtgcaggc tcctggacat ctccttcaac gatcgatgct aagcaaagag
     8941 agaaaaatat aatctccgaa ttttgatgtt cggaatagcc aaggccagtc aatacttgtc
     9001 gactaatgtc agtgactgca agagaagcca acagttcagc atcttttacc agttccatgg
     9061 atgtttttcc aattcgaatg gttgtcaaac tcaaataata agctaattca tccattcgat
     9121 ttggtgcgaa tcgaagatct gatagtgaaa tcgttttatc tagcaaatct ctaactttgg
     9181 cgtaataatc aatggaaagg gttgcggtta aatacgtgag cccaaatctt ccagcaagtg
     9241 actgcatcaa acgagcagtg cgattttttg cttctcttct taatattgct tctgacccta
     9301 gcagcctaaa accagttttc ctagtatacg acagctgaca atcttccact tctaattcat
     9361 ttcttatctt tttgatatct gacaggactg tccctttaga aacctgaaga aaatcttgaa
     9421 aatgaaaaac agaaacagac gttgcagtcg aataaaccat caaataaaga agatctcttc
     9481 gctcttgttc ggtatacaca gcttcaaatc gagtaatatc caaaaatttt tctgcccagt
     9541 cgattgctcg gaatttcttt atacggatac gggtatcatc cacatattcg gcaaaaggag
     9601 ttaattgttt gatcgcctcg cttgtattta cttcgatgat ctctgctgct ttttctagac
     9661 tgatttctct tggtgttttc aggagataac taattttgta gttcacagcg ctgttcacct
     9721 cctacttata ttctagtatg gtagcgcttt taaatcgagg ggtgtttggt acaacttaca
     9781 gttggatttt ttttacgact gtaccgaacg atttctatcg aatggataca gaaaaaaagc
     9841 aggtgaccaa atgtctcctg cttttatgaa ttgatttcgt ccgatcaatg atgatcatgt
     9901 ttattttttg aggtcagttc ttcaaatttt tctttgacat cttcaaccgc tttttcgata
     9961 tctttttcaa agtgattggg ctcattaggc tttgtttcct cttctacaga atttgaacca
    10021 gataaagctg tttctttatt ttctgtttca ccagagtttg tgccagctaa tgattcttct
    10081 ttgtcttctg tttcaccaga attagttcct gctaaagcca cttcttcttc tgttttaggg
    10141 gccattgaac ctcttaatcg tttgtgtttt tcaccgtcat aatttgtcat gttggttcct
    10201 cctagtagtt ctatactttt tcatcgtaca ataagacagt ttatgcgtca aaaaacaaac
    10261 ccgattctta caaatatgtc atatttttcc ttctgattct tgaatgcttt tgattccttc
    10321 ttccaaaatt ttcatttgta agattgtttc ttcatctttg atgacttttt ctgcgccatt
    10381 aactaatgtc tcgtaaatcc cttcgtagac acggctgtaa tcaccaacct cggaaattac
    10441 tttttcttca tgataactcc cttgttcatc tatataggtc aatacaccat aatgttcggg
    10501 caaatcgata ccaaagtctt catgatcagg catgtaaaac attttcagat gttcttcttg
    10561 tcgatctttt gtttgtttca cgaacatccc tttttttccg taaacgacga aacttggacg
    10621 ttctttgatg cggaaataac tagattttat cgaaattttc atatttccat agaaaaaatc
    10681 cagatcgaag taatcattca ttcgtccttt ccctaaaagt tgacgtacat catagctgat
    10741 atcatctggt tcgccgaaat aagaaagaac ttgatctact gtatgacaag catgtccata
    10801 taaatagctg ttcgcagtag aaaattcatg cgtattttct ggtacttccg gtctgaaata
    10861 atcataatgc atttctactt ctaaaatatc ccctaatttt ccgctttcga ttactttttg
    10921 gactgtcaag aagtcagaat cgaaccgtct gttttggtaa cattggataa atagaccttt
    10981 ttcttttgct aatgcaaata actcttctgc ttcctttgac gtttctgcaa aaggtttctc
    11041 tactagtaca ttcttaccat gttcgagtgc catttttcca tattcataat gaaattgact
    11101 aggtgtagtg atgacaacta attggatctc aggatcatcc caaagatcag acagctgatc
    11161 agtatattcg atgtcttcga atgttttcca cactggttca gatggggaaa aaattttttt
    11221 gatattgatt ttgttcgtaa gttttcttga aaaaggcaaa tgatagcgat ttgtactctt
    11281 tccattgcca agataagcga ttgtaagcat gagtggtgcc tccttttatt gatgttttca
    11341 gtatatccta gaaaagcgca gaaagtattt ataatagcta ttctggtaaa aaagttcaga
    11401 aaaacacttg ttatcattcg gtttggtgaa attaatgttc aaagtaagga taaaaatcaa
    11461 aaagatagta gcggaaccaa gtgtttatca gtaagataga atttggaggt cagatcatgc
    11521 ttattgaaga aaaactaaca aaacaagagt tgtttaccac gacagagaaa cggattgtcg
    11581 attacatccg taggaatatc gaagctgcag tctacatgac gattgaagaa ttagcaaaag
    11641 ctacgtatac ttcccattca gctatcattc gtttatgcaa aaaaatggga ttcagcggat
    11701 ttaaagaatt ccgtttcgag ctggggcggg aagttcatca attgattgct cagttcaatc
    11761 aaacgattga tcctaatttt cctttttcaa gtgaagatag cactcagcaa attgcaaaga
    11821 aaatggcgga tttatcgatc cagtcaataa aaaaagcaca attgcagatt gaacatgaag
    11881 atttagatca aatcggtaaa gtattgaaca aggcacagcg aatctttctt tttgcaaaag
    11941 gtgattctca aatcacggca aggaaattcc agaataaaat ggtcaagttg aataagtttt
    12001 tgatcatggc tgaagaatac agtgattctt cttggaatgc agccaatttg actagcgagg
    12061 attgcgccat ttttattagc tacagcggtc gcatccatca ttatgaacga ataatgacct
    12121 atttgaagca tgtagaggcc cctaccttgc tgattaccgg aaatcagcat tcagaaatgg
    12181 ccaaacaggc tgatatgtgt cttgttatat cgcaggagga atatgatttt gctaaagtag
    12241 cgactttctc ctctcaaatt gccttcgatt acgtattaaa tacgttattc tcagtgatat
    12301 attctcaaaa ttttgaagag aatatccgca acttgaaaaa caaacaagaa ttgatgcaaa
    12361 aaggccttct taaagaatat cggaaaactt aagaaaagat cctcatttag taataagaaa
    12421 gatagtgttt gataggctct ctttgatatc atcataaaaa caggctgaag ttttcttttt
    12481 caaacctcag cctgtttgtt tacggaacga ttcgaataga tatattatag aaatgaggaa
    12541 tagctaactt ctgtttctta ttttgaacga ttgacgaaaa agatatataa gaaggaggaa
    12601 aatcagtata catgcagcta acaatccata caggaaaaat cgtttgttct cggatacttt
    12661 ctgtacttct ttttgaactt cttttgcatg ataaggaacc cggtgccctc tgacaagtaa
    12721 gcgatgactg ttgaccatat aaggtgtaca agttaggagt gtcacgaaat cctgcccttt
    12781 ttcgattctc agatcttttg tatcagtcgg ttctaccgtc ttgatctgat cgacttgata
    12841 agcgagtgtc cgtccattga tttcgatata gaaagaatca cctaaagtca gtttcggtaa
    12901 gtctgtgaac aactttgctt gaggcagtcc tcgatggctg gaaataacgg catgtgtatt
    12961 ttctcccccc gtaggataag acgttccttc caataatgag gcaccttttt ctagcagcac
    13021 agtattcgtt tcatcaaaaa ttggcaaatg gacattgatc tcagggatcg tcaaaatacc
    13081 gatcgtatgt ttctcaaaat aggttctatc ctttttcgca ggttcttttt tcttagtaaa
    13141 gggatctgca cctggatttc caccttcttt tgctaattgc tggtttttct ttgtcatgtt
    13201 ttcttggatt ttagccatga cttcttcatt ttctttgacc gcttgttgct gataatgaga
    13261 gatgatctgc tgatccagat aattattcag cgcgtcgcta acaaatggat aagctagtat
    13321 ccctattcct gagagaagca agaaaagcat cagcagatct agaagtttcc ttttctttgg
    13381 tttcatttgt gttctccttg tgatgactgt ttgataataa aagaaaaagg agcatttgcg
    13441 taatccgctg ctcctttttc caattcttgc aaaaagacta aatctgactg tgcttgcgtc
    13501 tagcaaagta cagtccagca atcagtagaa ggactactcc tgcaccgata tacacataga
    13561 ttcccttacc gcctgttgaa ggaagtgtac ctttgtgttt atttggtatt ttttcaggag
    13621 aaatcaactg tcctgctgtt acataagatt gttcatcgat agtaaatgct acacggtttg
    13681 ttaatggaac atatttttct ggcgctttcg tttcttccag atagtacgtg ccatatttta
    13741 gacctgtcac atcgattaaa ccgttacttg tggttgtaaa aaccgttgct gattctttcg
    13801 ccgataccca gctgacggct tttgtggatg ggtcgatcga taaatatttc gctgtgtcac
    13861 tatcttgatc acgaacgacg aattctgctc cagcaagtgt ttggtctgat gtaacgtcac
    13921 catctacttt aacaaatcgt ttgcccccag taacgacatc gactgacggt ggtgtttgat
    13981 catttgtatg cccgttatcg acatttgctt ggttagaaaa tcctttggtt ggatctgctt
    14041 tttcgttcaa atgcatatag taaacgaatt tcaatgtacc gccaggagtt aatgaaggaa
    14101 tataattcgg atcaactgaa accgtgaatc cgtctgtttg ctcagtgaca gaataattta
    14161 ctgggtcgat ttctttattt ccatcatata aggcataagc atacgttcct gaagaatcat
    14221 tatcaaatgt taaagcagcg tcatgagtat cgataagttt gaatgttgtg tacttgtttt
    14281 gcgttccttc tttatcagcg atacctaatg ggatattgac agaaatctca tattgaattt
    14341 tttcaccgat tgcgacatct tttccattca attgaggtgt tgttttatca actttagacg
    14401 tatcattttt gatggtcttt tctactggtg tttggctatt tgccaggatc tcaaaaggcg
    14461 tgattgtttg cttttcaatc atttccgcat tatcaggagc ttttacttct tctaaattat
    14521 attttccaac ttctaaatga ttaacgatca actggccttt ttcaatagat gcctcggcaa
    14581 agtcattgtt gccgatgtca taagaatgac cagtaatgaa atgtttggct ttggtttgat
    14641 cagttgtcca agtgtacaat ccatctgtga cactttggat gtattttttg acgcttggtg
    14701 ttccttcttc tttagaaata ataaattctg ctccatttag tgcttcgttt tcggcagtac
    14761 cgatttttgt aactttcaac gttccatcat tacagactgt atttttaggg tagagatgga
    14821 tagtatctag ttcttctgtc ccgtatttat aagagccatc tgcttgtttg atcatctcat
    14881 atacagggaa agctaaaacc atgtttgcgg cagctgacac tccgtctttt ggttcttctt
    14941 tgatcgtgta gactgcatct ttcccatttt gttttttagg taaagataaa gtgacatttc
    15001 catcagcatc tgtcgttcct gaagcaactg gtgtaccagg agtcaaagac tggactgctt
    15061 gttttgctgc atccacggac gctcctttat ctcgttgcgc ataaaattct tgagtgacgt
    15121 tataaactga aaatgaaata tcggctaatc cttggtattg atcgaattcg ctcatttctt
    15181 tcccgctgtt ttggattaaa ggatcgggta aatcagtcat tttcttttta tgaaggatga
    15241 cttgagctgc tgtctcctct gcaaacactt tttttgcgcc gaagctgttt gtgagtaatg
    15301 gtaaaataag gaaaaggact cctaacataa cgtttatttt tttatgattt ttcatttttt
    15361 cactcctgtt ctttttttga taaacaacca gattgcaaag cttgcgaata ccataccaac
    15421 aacgcctaaa agtgtatttt tcgtttcgtt tgtttttgga agaaatggac ggctattttc
    15481 ttttttattg actactttca atatactgtc cgcctcttta tcctcagaaa gaaaagttga
    15541 aatggtaaac ggaatttctg tttcacttaa aatataacca gaaggtgctt cgatctcttt
    15601 taatcgataa cttcctgttt tcaacccgga aattgaaaaa gcgccatttt tatcggaaat
    15661 caggtgaagc gcatctgatt cgttctttgt ccacttgtat ccgttggctg tttgctggag
    15721 gtaatttcct gctttgtttt taataaggaa tactgcgcca gctaatcgtt tatctggttt
    15781 atttgtctcg attttgacaa attgcttact ccctgtatat acagagactt tctttgtact
    15841 gacaccaaaa ccaaaatcca gtgttccgtt gttgttgatt tccttgttcg cctgtgctgt
    15901 actgcttagc tgcatctgat aagaaatggt cagttttttt ccagcatatt tttccaacga
    15961 agggatcgaa aataaaacat caaatccgtg aggtttcttg tgtatgacaa agccttctgt
    16021 cagcttttct ccattcgatg aaatcgttaa actattaggt aaaaacgtta atgcttcatc
    16081 cgcactatct gacaattcga acttttgata gtcaaggata tttacaggaa ttgtcgtatg
    16141 caaggaataa gtgatttttt caccaatcgt aaaatcattt ctaggctctt ctaatacctt
    16201 ctcaaaaggt ggatcagggt agtcgttttc ttcattttta ggatacaaat ggatagttga
    16261 aagtttttga ttgttttgtc catgtacagg aagaacaact accatattct ctgccttttc
    16321 tttgacgact tctggtactt tggattcaat gaaaagatac gctttatctc ttttttcttg
    16381 atctttagcg gccagagaaa aagaagcgat accatcttcg ttgttaagag ttgtagttgt
    16441 tgcttctgcg gtaaacatac cggaagacgc accgtttttt gcgatctctg cttgtgcttc
    16501 ttctaccgtt ttgccctttt cccgtagatg gtaaaaagaa tctgtgacat cataaacttg
    16561 gaatgtgaca ccatttaatc ctcgatacgt ttgtaataaa gctttttctt gtccgtcatt
    16621 tggatgattt ttcggcagtt gcccgtttgg gaaaagcaat ttgtgtaaag taatctgaac
    16681 catttctgtt tctgtttctg ttgctacctg agtaaaagct ggtttaaata gtaacaagaa
    16741 gagacacata ctaagccaac caagtttttt catatttagc tgccccctct ttcttttttc
    16801 aaaaaaagat accaaataga aaacgcacaa ccaatcatcc ctactagata gattcctaaa
    16861 cggccaattc ctcccgtttc aggtaatggt acttttgcct gattcgtgat gtctaaagaa
    16921 atctggttgt ttttggctcc tggtgacaga acagattcat gatcttgtcc atccacttga
    16981 attgacccat cttcgtgtat aactatagta actggctctt ttagaccttg gtatccttct
    17041 ggtgtaaaag tttcggtcag cgtataagtt ccaggtttta aattttcgaa tagaaaggta
    17101 tctgtttctt gtccatcttt tggcgattct aagtccattt ctggtccctg cagtctgaat
    17161 tttgctcctt ttaatgtctg tccgttgtct tcttttttgt gtaccgttag atcaaacgct
    17221 tttagtcgat tttgatgagt gagcgtccat acattatttg tcatctcggt attttttccc
    17281 gtgtaaatga tttttccata tttatcgatt tggaattcat aatttcccgg cgaagtatca
    17341 tatccggcag gaccagctgt ttcaacgact cgatatttac caggtgtgag tgaatcaaaa
    17401 ctggcaagac ctttttcatt cgttgtttgg ctgtcaattg gctgataagt accatttgct
    17461 tcttttttct gtagggaaaa tgctgctcct ttaagattta tctctttgtc cgtcccttgc
    17521 atcgcatact tacgtactgc tacaggaact tcaacaaaag gattttcaat agtcaactgg
    17581 atcgtatcat cggaagtagt gactgttttt ccatcaatgg ttaccgtacc atcagaagcg
    17641 atcttgattt cccaagtagt ctttttgctt aatccatgcc cttctggagc ttttgtttct
    17701 gtcagcgtat aggtcatttc tttttgcaat ttgacatttt ctggaagaga ataggttccg
    17761 tcgccatgat ctgttagtaa agtatcaata gaatctcctg ttaatttgaa aacagcgcca
    17821 ataagatcct tttcacctgt agaggaattt ttcgttattt tcaagtttaa cggtgtgaat
    17881 tgtttagtat ttttccatgt cattgcatct atttgttgag aatcgtatcc tggtacaggt
    17941 aattctttga ttgtttggta actgaatgct tgaccttgat tattgtattg aggtagtgat
    18001 aatatctctt gataactttc tccgctattt gcagataatt tgtcaatgtc tgcacgttcc
    18061 cacgtatttg ttgtatcttt agctggttta atgattcgaa tatatccgtt tttccaagct
    18121 gcagcatttg tcgtatgttc ccgttgaatc tcaaaagtaa cttgatctgg acgatcagct
    18181 agattgttgt caaattcttc ccataacttt ttgatgtgaa gactgactcc gggagcttta
    18241 gcagatggta taccgaattc agctaattca ttggtatcaa tgtttggctg gaaagttgtc
    18301 ctgccgttca tttgatacca gaaatttgga tggaagtcct cattttctgt ttggattctc
    18361 acttggtaat ggatttggat ttcttggtct tttcctaaat agatctgatt gctcttgatg
    18421 gtatttcctt ctatggaaat agatggagtg accgttgtag gacttgtccc cacactcttg
    18481 actgaaagtg taccaggctg ataaatgaat ggttctgcga ttggatcatt tatttgtcca
    18541 ttgcttacag ttgctgagat ctgtacagct tttttggcta ggtattcaga gatatctgtt
    18601 gcatgatcag ctgattcata gtaaagatcg cctttttcat ctgatgaaac catttgtcgc
    18661 atacgagact ctacttctgc ttttgagaga ccagctgccg gatcgctttg aagttggatg
    18721 ccaagaccat gtatttcgat tcctcgttct ttgagtgcca tcgcttctcc gatggttgcg
    18781 ataaacgtac tgtcgattct tctggataga ttgttttggt caggtgcatc atagattctt
    18841 gagattagag aagtatttcc cggccgatct tgcgtattag aaaactgagt tccgtaataa
    18901 ttgctgcttg attgtgcgtg tacccgctgt actttatagg aaaatgttgg tacaccatcc
    18961 gtcagcaaaa cgatcacttt tttatgtcca tttggaacgg atagcatgct tcctgcatct
    19021 cttagtgctt tttgtgtaaa agtaccacca tttgtccgtg aaggtgtaat ggattttact
    19081 tgatttttca ctgaatcaaa tgaacccatc tgtactgcac cgttactgta gctatatcct
    19141 tcgcttgagt agccgacata tcccatattg attttgtctg tgataccgct atctgctaaa
    19201 gtatcgacaa aacgatcgac accaatcttt acttcaccga tccgattatt gtcgttcata
    19261 cttcctgacc agtctacgac caatacgaga tcaagaggcg tgatatcttt ttgtacattt
    19321 ccacgagcat tcaaataaac atcaaacaat ccgggtgtac ttgtttcctt tgcatacttt
    19381 cgtatcgcat agtctgcttg attaccggtt cctccatatt cgatatacga atgggtccga
    19441 tcattaggat ctccattcca agaatttata ccatcccatt gatttgttcc gtttttattg
    19501 ccttgatgat caagaacatt tgtatttcct gtaggttgcc aactggcttt aggataagtg
    19561 ccctgatcat ctgttgtata ttctgtagga atcgtttgat tagatggcac actcacagct
    19621 ggcaaggata atgtaggcaa tgtaaatggg ctgacagaat tttcgttttc ttttgatgat
    19681 tcttcttcta tttcgtctgg ctttggtact gacacatctg tatctttctt attttctgtc
    19741 tcatcaataa tgtcatattc tttattatcg acgatcagct tgtttgcctg gctgtggtaa
    19801 agtgctggat cgattggaaa actcagatcg atagtttgtt tagtgtttga tgagatttcc
    19861 aacgtaattt tcccgctatt tacctgataa gcatagccat ctccttcttt tctttccaca
    19921 tcacttaagt aattttcgac tagaccaatt tctttttcta tcttttcagc actggtgtta
    19981 ttcgcattta gcttgatgtt cagtgctttt tttccagact gatccgaaat gacttcagct
    20041 gaaattgttt ctaaaactgt tggatcatct gtagtgtctg ctattgcaga taatggcaca
    20101 aagtttgata aactcaatat tatcagcatg aaaataactt tcagtttctt ccctgtggtc
    20161 gtcatttttc ccctcctttt tctttttttc tgttttgacg tgaatagtga aaattattaa
    20221 aataaacagg cgttacataa ttttatttat tttaatatga taattattat acgaagggcg
    20281 ctaaagggaa tcacaccaat tttcatacca attggtttca tttatgaagt tgattccttc
    20341 gattaatgag cgtttcattg aaagaaaagg gttgagggag aaaaacatat gatcaggttg
    20401 ttggaaagaa actatagaaa gatggaaaaa atcattcgaa ttctgtccat ttctgacaag
    20461 tggatgacta aaaaagaact tgccaaacga attggtagtt cagaatctac atttgtaagg
    20521 tatatcgaag agatcaaaat ccgctgggga acggatgtaa cgattcaatc ttcacaaaaa
    20581 ttggggtatc gtcttgaaca tttgaatgta ccagcttatt taaatgttct gatggaaatg
    20641 tcgcaaagtt ctacaaacag tcgtttatta aatgagatta ttgtcaatcc gggtaaaacg
    20701 attgagtatt attgtgaaaa aatttccatc agccggtctt cttttacacg taagttgaaa
    20761 caatgcaatc aaattttaga aagctattta ttaaaagtga tcgttgacca aggttttcag
    20821 ttaactagcc ttaaaagtga gctccagttg cgtatctttg ttaccttttt ctttttaagt
    20881 tactacggaa gacgcacgtt gccatttcca atcgaaaaaa agaagatcaa agaaattatg
    20941 gtcaagaata attgtcagat gaatttgttt caggaagaaa gcccatatga gcaatctttc
    21001 tttattatgt attttgttgt gcatctcatg agagaatccc aaggaactcc atttgaatgg
    21061 aaagctggag actcttatgt acagttgata aatatcgata aaaaagactt cttaatcttg
    21121 aaaaatctct taggaaaatt gaagtacagc agctatataa aagtagtgaa ctattttgtc
    21181 aatacgattt tttcagaatt ttcggagaaa gaaaaaaatg aaatcagtga gcagatacga
    21241 aaaaatatgt cctactgttc gttttcatcc gctgattgca aagaaaaaaa cgaagaatat
    21301 gttgttcaat tattaacgca aatttatttt ttctcccgta ttttcccata cgatacgact
    21361 catttgacga tccgtgcaac ttactttgca cagcagatgc aacaccgcca gatacgtcta
    21421 tatgctggat ttcgtgcaga acttcaattt tattccactc tactgaatca gaatctgatt
    21481 tattatttat ctagctatat cttttggatg cttatcggta atcctgaaat tcaccattat
    21541 acttctgata aaaaaatact gattatcagc gacttaagtc ttcgacatag tcaatatatt
    21601 gaagaatata tcagtgatat tcttgctgtg cataaaattc actctgagac aactgctatt
    21661 actgaagatc agctgtcgaa atacaatctt ctcgagtatg atctgatcgt tacgaatcaa
    21721 cccattttga atgcccatgt tcctcatata ttgattgatg atagtgtgtc attcgcaaat
    21781 gaagaagaac tcatccgctt attcgagtta tgaataatca aaaaacagct atgtataaac
    21841 caccctgata aaaatttcag gacaaataca catagctgtt tctttttatt gtattccttc
    21901 agcaatccaa cgagatttat agcgaaagtc ttttaacaac tcttcttttg tccaattatt
    21961 tgcaatgaga tccgtcaaag tgttcattac gagggtcatg gaaaagtata ggaaactaac
    22021 tgcttcttcc gacgattgaa tggagaaatg attgattctt aataattcca gccatttttc
    22081 gttcattgga gaatcagccg atagttctct acgtttggtt gtctgctgat catttcgctg
    22141 ggttaacaag tataaccttt gccaatagat actttcgtga tctaagtcgc agcaccactc
    22201 taaatatttt tcgatcccca aaaagaaatg ttgtttgttt tcgctaatga attttaagat
    22261 atcttgatgg actatgtgag aactgctttt gatcgtatac aaataagcat cttccaaatc
    22321 ttgaaaatat ttataaaaag caccgcgcga catccccatc ttgtcaacga tttctgagac
    22381 tttgacttgg ctgatgtgct gtgagcaaaa tatctccagt aataggtcat cgattctctg
    22441 tttctttgcg ttcgataaat tgaaaaaagt ttcttttggc atccacatct ctcctttatc
    22501 tctgattata cgtggatagc ccttcattgt catgaaggat tcattccttc tttttctctt
    22561 cttctgtcaa gtacccatct tccattcgat aaacgcgatc actccattga atcatacgga
    22621 catcatgagt aaccataata gtcgcttttt gtttctcatg agcttcttta actagtaatt
    22681 tgacgacttc atatgcatga tctgtatcca aactagctgt tggttcgtct gctaagatca
    22741 agcttggctc attatataaa gcgcgagcaa ttgccactct ctgtctttct ccaccagaaa
    22801 ggtctttagg ataactattt ttaagtgact ggatatctaa agattgcagc aaggaatcga
    22861 tttttgttga atcttctttt tttgaagtaa cttggtcaac tagttcaaat tgttccttta
    22921 ccgttaaaaa aggaatcaaa ttagagcttt ggagaataaa tcctatttct ttaaagcgta
    22981 aatctgctcg ttttttttct ggaagctcag ttagtgagtg accgttgatc actatttctc
    23041 cggaagtcgg tgtttgcaat cctcctgaaa tggtaaggaa agtactttta cctgaaccag
    23101 aaggaccaat gatgctgacg aattctcctt cctttacata aaaatcaatt gctttcaaag
    23161 ctgagacctt tgtatgacca gaaccaaatg ttttggtaac ttttttcatc gttaatattt
    23221 ttttcatttt tattaacctc ctattgctgt aattgggtct actttggtta tcgtacgtat
    23281 cgaaaacaat ccgcctagca ttgcgacaat gatcaaaatc ccactatata gcaaccattg
    23341 tgaccaaaaa acagcaaaag gcatcgcgct tggtaaaatc agactagtca aataagcaaa
    23401 caaaagagca agcagaacgc ctaccacccc aacaataaag gactgtgcta ctaaagattt
    23461 agcgatgaag aaatttttga tcccttgtgc tttcattact ccaaaaatag cagtcttctg
    23521 aagtgtcatc acgtacatga aaataccgac cacggcagca accactaaga acaaaaagta
    23581 tatcatcgca tttaaggtca tattttgggc agaatagcca ggaatacttt caatgaatgt
    23641 cccaatagtt aataactgta gacccttcga tgctttttct ttcgaaattt tagctgaatc
    23701 ctttattacg atcccattga tcggctgttc ttcttcagaa gcaaacggct gttgtccata
    23761 cttcaaagca gtccacgtat ctagggacgc atagatgacg ggagaaactg tgtaataagt
    23821 ttcaggaaag ataccgacaa tcgtcaaagg atcgtcgtaa ctgccaattt tgatcgtatc
    23881 tccaatttta tagcctgaat cagccagatt ttgagagatg atgatttcat tctttgtttt
    23941 aaattcatgc cctttcgtga gttttggtaa taaaaatgct tctttcgttg tgccgaaaac
    24001 tgtaacattt tctttgtttt ttcctttgat tgctccgctg tacaatccga ttggtgcttt
    24061 ctctcctcct tcaatgtatt tcagatctcc tcttgttaat tgtgaagctg caaatacttg
    24121 atttgcatcc tccgacaaaa caatctcttg tgcgtcccaa tcatcgattg cttttttaaa
    24181 ctcttctgaa agtccattcg ctagtcctga caacatgaat acgacatacg caatcaagaa
    24241 cataataccg acgatcaagc catatcgtaa tttagaatag cgaatttctt tgatagctag
    24301 aaacatttgt ttttcctccc tatgatagat tgatagtgac aaaatgtcac ttgttgaata
    24361 gtgtaactct aaagtgacaa tttgtcactt gtgttgtttt tttcttatca ataaattgtc
    24421 taagacatct ttcgataaaa ccttaacatg agtcataaaa aatttattta agccttattt
    24481 atgctttcca tgataaacta aagtgaccaa atcaatgagg tgggaaaatg attaaactat
    24541 tagtagtaga agacgataaa gtattatcca ataatataaa agaaatactc gctgcaatcg
    24601 gcgagataac acaagtttat gatggtagtg aaggactgtt cgagttggaa agtggagttt
    24661 atgatcttgc aatcttggat ttgatgatgc cggaaatgaa tggatatgaa gtgttagcaa
    24721 aaatacggaa agaaaaaata cagactccag tattgatttt gactgcaaaa gatagtttgg
    24781 aagataagat tgaaggattt gaacgaggtg cagatgatta tctaacaaaa cctttctatc
    24841 gagaagaact gatcatgcgc gtgaaagcat tattgaaacg ctcgctaggt ctctttgatg
    24901 aaaatgttct gaagtataag gatatcagtt gtgatttaaa aagcaatgaa gtcttgtata
    24961 aagaacaagt tttgcccata caaggaaaag agtttgaatt gctcgtttat tttttacaaa
    25021 ataagggaat cattttgaca aaagagcaga tatttgaccg gatctgggga tttgattctg
    25081 atacgacgat tacagttatt gaagtttata tgagcaactt acgaaaacat ctgcgaagta
    25141 ctggattgga taaagaaatc aaaaccttac gtaatgtcgg ttatatactg caaagggtgt
    25201 gatgcaatga atggagatat tacacgaaag caacgtagtc gcttttttgc gacaaatgta
    25261 gcagcatttg cttttatctt ttttcttttg ggattcatta ctttacagat attaaaccaa
    25321 tctgcataca gagaaacaga tctgtcatta gagaaaataa attttgattc tcgaacagta
    25381 caattagaga tacaaagata tcagcaaggc gatccctttt taaacccgac tcaaacggaa
    25441 caaaacagaa caaacatgga acagccatta atggaaccat caaatgaccg ttttaacact
    25501 caagtgatct tgtggtctaa aaatggtgag atattgaata aagcagctat tggtgggcgt
    25561 ttcaaccaaa tcaaagaatt aaaaatggat acgaaaaatc tgaataccat ccaaaatatt
    25621 gaattaaatg aagacagtgg ctcatctgcc ctctctttcc gttcgattac gaaaaaagcg
    25681 gagacgaatc aagatggcgt tgcctacatc caaattttag aaaatacaaa tcaaatcaag
    25741 aattcattag cgacttttcg cacgattatg attttgtgta tgattgtttt ttggattctc
    25801 tctattggta tcagttatta cttgtctagt ctaagcatgc gccctattat tctttcttgg
    25861 agaagacaaa aagaatttgt tgagaatgct tctcatgaat tgcgcacacc gttaacgatt
    25921 attcaaaata gtttggaaca tctttttacc aagcctaatc atactattat tgaagaatcc
    25981 gaaagcatcg ctcaagcatt gtcagagacg aggcgcctca ctggattgac ttcggatttg
    26041 ctgacaatcg ctcggaatga ttctgatcag cagctgcttt caaagcaaat gatcaataca
    26101 caagaatata tcgaacaact tgttaaacca tttcaagaga tggccatcat agatggaaaa
    26161 gagtttattc tccataacga tgcaacatta gaagtaccaa ttgatgaaaa aaaaatccat
    26221 caagttttgg ttattttgct ggataatgca ttaaaatata cgaatccaga agataagatc
    26281 attttggatt catctgttaa ccataaagaa tggctgattc acgtaaagaa tactggccct
    26341 tctatcagtg aagaagatca agcaaggatt tttgatcgat tttatcggga agatcaatct
    26401 cgggcgaaag agactggcgg atacgggctt ggcttggcaa ttgcaaaaca ggtaatcgaa
    26461 cagcatcatg gacagatttc tgtatccaat tatcttccac aaggagtcga ttttaaagtc
    26521 caattgccgt tgaagcctaa aaaataatgc ttatccctaa aaaagggctg tgacaaaaat
    26581 cttgtcacag cccttttttg ttgcgaataa acggtgttcc agaagtagct tcttcggaaa
    26641 taagccgaaa tttctttaag ctgaccactt ctgtcacaac ctctttagct tacgcttacg
    26701 attttttatt gcggtcttca atgtgtacat caccgcttgt gtcaatatcc aattcttctc
    26761 ggctaacatc ttctgtcact ttttcaacat cttcatgttc ttctttatga atcccaactt
    26821 cttcacgaat aactgtatgt ttgcttacat ctacttgctc ttctttgatt gggatagtaa
    26881 tcgtgtcatc ttccaatttt tcgtctgttc cctgtgaaga ttgatctgtt actggtttgc
    26941 gttcgatgac gatttcttca cgtttgaccg gtacttctac cgtttcagta tcgttgacca
    27001 catgtttgtg gatgtcgact tctccagttg tcacatcatg tgtatttaca tctaattttt
    27061 cttcttttag ttgaattgta tcatcatcta gatttggttt gcgatcgatg tcttcagtct
    27121 tgcttgtacc cattgtatca gtctcgccag gtacgtccat cggatcagtt cctggtgtca
    27181 tattcaatgg atccgttgta ttttgaaagc ctgtcgtgcc acctacgtcc attggatctg
    27241 ttccagtgcc agtttctaat ccagtcccca ttgtgcttgc tgggctaaca gtatcttgtg
    27301 tatccattgt atcttctgtt gtttctggct gacgatagcc ttttactgtg atgacaaatt
    27361 ttccatcaga aatatctcct ctatattgat ataaaggatc attttctggt gtagccgtct
    27421 cagaatcata ggtatcaaaa gaaaaagtat cttttatttt ttcccataaa gaacggtctt
    27481 cttcaccatc catccgattt tcatctgttt caacatctat accagacaat ccgtcaaatc
    27541 ctagtctaga tgctgcttca tcagtagtat aaataacgat atcatctcgt tcataccctt
    27601 cgttacgcaa ccgttcaatt actgataatg cttcttccct cgtactatag cttccgatga
    27661 ccgttaaatt tttctcttcc atgtaaattc ctcctagcaa ata
//
LOCUS       DAZKCY010000028        26628 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000028 DAZKCY010000000
VERSION     DAZKCY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.028"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..168
                     /locus_tag="ACG8XC_001078"
     CDS             <1..168
                     /locus_tag="ACG8XC_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFP9190791.1"
                     /translation="LCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(228..581)
                     /locus_tag="ACG8XC_001079"
     CDS             complement(228..581)
                     /locus_tag="ACG8XC_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9190792.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1086..1508
                     /locus_tag="ACG8XC_001080"
     CDS             1086..1508
                     /locus_tag="ACG8XC_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFP9190793.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            1505..1735
                     /locus_tag="ACG8XC_001081"
     CDS             1505..1735
                     /locus_tag="ACG8XC_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9190794.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            complement(1961..2212)
                     /locus_tag="ACG8XC_001082"
     CDS             complement(1961..2212)
                     /locus_tag="ACG8XC_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190795.1"
                     /translation="MSVCFIKSSFPNHLFGFHKSCFTSNRTALAKLTGVPPLHGSHVA
                     ILIACDGFITLGLLTVWEYHYHDKNVVAGNPAKDCFSDH"
     gene            2196..2399
                     /locus_tag="ACG8XC_001083"
     CDS             2196..2399
                     /locus_tag="ACG8XC_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9190796.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            2481..3698
                     /locus_tag="ACG8XC_001084"
     CDS             2481..3698
                     /locus_tag="ACG8XC_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9190797.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     regulatory      3927..4006
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            4004..5197
                     /gene="metK"
                     /locus_tag="ACG8XC_001085"
     CDS             4004..5197
                     /gene="metK"
                     /locus_tag="ACG8XC_001085"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP9190798.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            5327..6799
                     /locus_tag="ACG8XC_001086"
     CDS             5327..6799
                     /locus_tag="ACG8XC_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP9190799.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            6882..7670
                     /locus_tag="ACG8XC_001087"
     CDS             6882..7670
                     /locus_tag="ACG8XC_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9190800.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7737..8405)
                     /locus_tag="ACG8XC_001088"
     CDS             complement(7737..8405)
                     /locus_tag="ACG8XC_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFP9190801.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            8520..10232
                     /locus_tag="ACG8XC_001089"
     CDS             8520..10232
                     /locus_tag="ACG8XC_001089"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190802.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            10235..12004
                     /locus_tag="ACG8XC_001090"
     CDS             10235..12004
                     /locus_tag="ACG8XC_001090"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190803.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRVTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(12209..12775)
                     /locus_tag="ACG8XC_001091"
     CDS             complement(12209..12775)
                     /locus_tag="ACG8XC_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9190804.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(12775..13731)
                     /locus_tag="ACG8XC_001092"
     CDS             complement(12775..13731)
                     /locus_tag="ACG8XC_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP9190805.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(13731..14387)
                     /locus_tag="ACG8XC_001093"
     CDS             complement(13731..14387)
                     /locus_tag="ACG8XC_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFP9190806.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            14757..16775
                     /locus_tag="ACG8XC_001094"
     CDS             14757..16775
                     /locus_tag="ACG8XC_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9190807.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    16845..17051
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17202..19616
                     /gene="leuS"
                     /locus_tag="ACG8XC_001095"
     CDS             17202..19616
                     /gene="leuS"
                     /locus_tag="ACG8XC_001095"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP9190808.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            19964..21517
                     /locus_tag="ACG8XC_001096"
     CDS             19964..21517
                     /locus_tag="ACG8XC_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFP9190809.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(21898..23682)
                     /locus_tag="ACG8XC_001097"
     CDS             complement(21898..23682)
                     /locus_tag="ACG8XC_001097"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190810.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLYSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(23697..25418)
                     /locus_tag="ACG8XC_001098"
     CDS             complement(23697..25418)
                     /locus_tag="ACG8XC_001098"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190811.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            25518..26279
                     /locus_tag="ACG8XC_001099"
     CDS             25518..26279
                     /locus_tag="ACG8XC_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9190812.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IRERKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         8590 a         4728 c         5473 g         7837 t
ORIGIN      
        1 ttgtgtcctg tatgtggaaa taaaacacga ttaaagataa gggaagatac tgaattaaaa
       61 aaattccccc tctattgtcc gaaatgcaga caagaaaatt taattgaaat aaagcagttc
      121 aaagtaactg tgattacaga gccagacgca aagacgcaga gccgataaaa tgagattaat
      181 acaatctcat tttatcggct ctttccgtta tgtatggatt cttttaatta gtcttcgatg
      241 tttcttgctt cgttgatacc gctggctaaa gattccatta aggatagttc tttgtctgta
      301 aagctatcca tgtatttctc tatctgtaat cgtcgggtgc tttttaccaa gttattagca
      361 ggtaagaaaa attcatcaac ggaaacatga agtaacgata caaggtcata aagaacttgt
      421 atgctggggt gttgcccttt attttcaata ttagttaagt accgtgggtc aatttcaatc
      481 aatgctccca cttgttcacg agttaaacct cgtttcaatc gagcttcttt aatggctaaa
      541 ccaaaggctc taaaatcata tttatcttct tttttacgca tagtagacca cctctataca
      601 ttttattgtt cctactgaat taaaaacagg tatagaaaaa cgtgttatat ggtttatagg
      661 tttatattta ataaaaagca ctactaaacg ccaataaaaa aaaccgttat atggtagtgc
      721 tatttacgct gttaaaatat tgtatattac ttccaaatgg cggtttgttg gaggtcaacg
      781 tcgccatgaa gtacatcata tacaataaat ttccttacat tgggttcttg tcaaaaaaag
      841 tcgtctatct gcaatagata agtacgtcca ccaatgtggt tttataaatc atatagatag
      901 aataacagaa gcatgtaaac agagaaataa atctgtttat atgctttttt ggctattcag
      961 aactttttta caaagtttat ttatcagtaa tgcaacaaat ccccctttca cattgggact
     1021 aagagtgaaa ggagataaac gagcaaggct cacttccttt cctagacaga aagggggtga
     1081 gaaacatgaa accatcttct tttcagacca caatagaaaa tcagtttgac tatatctgta
     1141 aacgtgctat ggaagacgag cgaaagaatt atatgcttta tctttcaagg attgcaaagc
     1201 gtgaggtgtc cttttcggat gttggcgatt atcttgttag ccagtttgcg acaacagata
     1261 actattcaac tgactttcag atttttacac tcaatgggtt atcagtaggc gttgaaaatg
     1321 atttgttgag tgaagcatta cgtgagttgc cagacaagaa acgtgaaatt ctactgctgt
     1381 tttactttat ggacatgagc gattcagaaa ttgcagacct gttgaaattg aaccgttcta
     1441 ctgtctatcg gcatagaacc agtggactag ccttaattaa aaagtttatg gaggaatttg
     1501 aagaatgaaa acacaatatc ctatgattcc ctttcctctc attgtaaagg caacagatgg
     1561 cgataccgaa gcgattaacc agattctaca tcattacaga gggtacataa cgaagcgttc
     1621 cctacgactt atgaaagatg aatatggcaa tcaaagtatg gtcgttgatg aagtcttacg
     1681 tggaagaatg gaaaccagac tgattacaaa gattttgtca tttgaaatta agtaatatcc
     1741 tctctccttt cgtggaagcg tgctaaacca ttccacgctt cccgaacagg gaggtttgtt
     1801 attccaccaa agcatattga gctttcaatg tgttttgata ggctaacgag ccattgttct
     1861 ttgaaaactg aataaaagta atcgaatacg tttcgataag aaaagagcca acggaactaa
     1921 ccgccatgac ctatcttata aagatagcga gcgattcatg ttagtgatcc gagaagcaat
     1981 ctttagcagg attgcctgca acgacattct tatcgtgata atgatactcc catacagtca
     2041 atagtccgag cgtgataaaa ccgtcgcagg caatgagtat ggctacatga gaaccatgca
     2101 ggggtggaac tcccgtgagc tttgctaaag ctgttcgatt gctggtaaaa caacttttat
     2161 gaaatccaaa taagtgattt ggaaaggagg attttatgaa gcagactgac attcctattt
     2221 gggaacgtta taccctaacc attgaagaag cgtcaaaata ttttcgtatt ggcgaaaaca
     2281 agctacgacg cttggcagag gaaaataaaa atgcaaattg gctgattatg aatggcaatc
     2341 gtattcagat taaacgaaaa caatttgaaa aaattataga tacattggac gcaatctagc
     2401 gtcgccaaag ggtcttgtat atgataaaat agtattaagt cgtatcaagg ctctttccat
     2461 aaaggaaagg agcaaatgcc atgtcagaaa aaagacgtga caataaaggt cgaatcttaa
     2521 agactggaga gagccaacga aaagacggaa gatacttata caaatatata gattcatttg
     2581 gagaaccgca atttgtttac tcgtggaaac ttgtggctac agaccgagta ccagcaggaa
     2641 agcgtgattg tatctcactt agagagaaaa tcgcagagtt acagaaagac attcatgatg
     2701 gtattgatgt tgtaggaaag aaaatgacac tctgccagct ttacgcaaaa cagaacgctc
     2761 aaagaccaaa ggttagaaaa aacactgaaa ctggacgcaa atatcttatg gatattttga
     2821 agaaagacaa gttaggtgta agaagtattg acagtattaa gccatcagac gctaaagaat
     2881 gggctattag aatgagtgaa aatggttatg cttatcaaac catcaataac tacaaacgtt
     2941 ctttaaaggc ttcattctat attgctatac aagatgattg tgttcggaag aatccatttg
     3001 actttcaact gaaagcagtt cttgatgatg atactgtccc taagaccgta ctaacagaag
     3061 aacaggaaga aaaactgtta gcctttgcaa aagctgataa aacctacagc aaaaattatg
     3121 atgaaattct gatactctta aaaacaggtc ttcgtatttc agagtttggt ggtttgacac
     3181 ttccagattt agattttgag aatcgtcttg tcaatataga ccatcagcta ttgagagata
     3241 ctgaaattgg gtactacatt gaaacaccaa agaccaaaag tggcgaacgt caagttccta
     3301 tggttgaaga agcctatcaa gcatttaagc gagtgttagc gaatcgaaag aatgataagc
     3361 gtgttgagat tgatggatat agtgatttcc tctttcttaa tagaaagaac tatccaaaag
     3421 tggcaagtga ttacaacggc atgatgaaag gtcttgttaa gaaatacaat aagtataacg
     3481 aggataaatt gccacacatc actccacata gtttgcgaca tacattctgt accaactatg
     3541 caaatgcagg aatgaatcca aaggcattac agtacattat gggacatgct aatatagcca
     3601 tgacgctgaa ctattacgca catgcaacat tcgattctgc aatggcagaa atgaaacgct
     3661 tgaataaaga gaagcaacag gagcgtcttg ttgcttagta gtacaaatga atttactact
     3721 tatttaccac ttctgacagc taagacatga ggaaatatgc aaagaaacgt gaagtatctt
     3781 cctacagtaa aaatactcga aagcacatag aataaggctt tacgagcatt taagaaaata
     3841 taaaaagata attagaaatt tatactttgt ttaagtataa aaaatgaaaa aatacgagca
     3901 gaaaactgtt ttatctgcta tacttggttc gttgcaagtt cccgaaagga tccgcaggac
     3961 ggagatgcct tgtaaccgaa tttaactagg gggaatcatt ataatggtag aaagacactt
     4021 atttacatca gaatctgttt ctgaaggaca tccagataaa attgctgacc aaatcagtga
     4081 tgcaatcttg gatgcaattt taaaacaaga tccaacagca cgagtagcgt gtgaaacgtc
     4141 tgtaacaact ggtcttgttt tagtatttgg agaaatatct acgacggcat atgtagatat
     4201 tcaaaaagtc gtacgggaaa caataaaaga aattggttat acacgtgcaa aattcggatt
     4261 tgatggagat acagcagctg tattggttgc gattgatgaa caatctcctg atattgctca
     4321 aggagttgat gaagcacttg agatccggga tgaagataaa aaagacgtat tagatgagat
     4381 tggtgctggt gaccaaggtt taatgtttgg gtttgctgta gatgaaacac cagaattgat
     4441 gcctttacca atcgctttga gtcatcgtct ggtacgacgc ttggcagatt tgcgtaaatc
     4501 aaatgaatta acgtatttac gtccagatgc aaaatctcaa gtaacggttg aatatgatga
     4561 tcaaggacaa ccggaacgcg tagatacaat cgttatttca acacagcatg atgatgcagt
     4621 agataatgaa acaattcgtc atgatgtcat cgaaaaagta gtgaaggaag ttattccagc
     4681 tgaattatta gatgatcaaa cgaaatatta tatcaatccg actggccgat ttgtcattgg
     4741 tggtcctcaa ggggatgccg gattaacagg aagaaaaatc attgttgata cgtatggcgg
     4801 ttatgctcgt catggtggcg gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc
     4861 tgccagctat gctgcacgtt atattgctaa aaacattgtt gcagcagggc ttgctcgcaa
     4921 agcagaagta cagctagctt atgcgattgg tgttgctcaa cctgtttcga tctcaatcaa
     4981 tactttcggt actggaacgg ttccggaaga agaactgatt gctgcagtga gagaaaactt
     5041 cgatcttcgg ccagcaggaa tcatcgagat gcttgatttg cgtcgtccga tttacaaaca
     5101 aacagctgct tacggtcatt ttggtcgtac agatgtagat ttgccttggg aacatacgga
     5161 caaagtagat gcgttgaaag caagtttagc aaaataagtt atattcagct ggaacgatcg
     5221 ccaagtggtg gttgttccag ctttttttat ggcagttaac ctcttgaaaa agatagtaat
     5281 tatttatcta tttttagatc ctgtcataat gaaagggagg aataagatga aaaaagaaac
     5341 aaatgttact ttggtcacag tgagtatttt cattgcaacg ttcatgacgg ctattgaagg
     5401 tacaatcgta acaaccgcta tgccaacgat tgtcggttca ttacatggaa tggaaatcat
     5461 gaactgggta ttttcgattt atttgctgac aaacgctatg cttactccga tttacggaaa
     5521 acttgcggat aaagtgggac ggaaaccaat ctttattttt gggacattat tgtttattat
     5581 cggttcgtta ttttgcggtt tatctcaaag catgattggg ttgattgctg ctcgtgcgat
     5641 tcaagggatg ggtgctgggg caatgatgcc agtagctttg acgatcatcg gcgatcttta
     5701 ttctattgaa aagcgagcaa aagttttagg tttgaatagt tcagcctggg ggattgccag
     5761 tgtttttgga ccgcttgcgg gaggattcat cgttgaaacg atcagctggc actggatctt
     5821 ttttatcaat gtgccgatcg gaattctatt aattattttg atttggctat atttaaatga
     5881 accaaaaatc atacatgagt ctaaaccaat ggatattttt ggtagtttta gtttgatggc
     5941 catgcttgtc tcattgctgt tagggttcca attattagga gacgaaggaa tgagtccgct
     6001 tgtttctgct ttgctgctag gtagtatcgt aatgttaggt atttttgttt ttgccgaaaa
     6061 aagagctaaa gatcctgtca tcatgatgca tttattcaaa aatccactat ttgttgtggt
     6121 caacttggct gcagcattgg ctagtggttt tttgatggga atcgatgttt atatccctat
     6181 gtggatgcaa ggtgtattag ggctgaatgc agcaattggt gggctggtat tagcaccatt
     6241 atcactagtc tggatggtcg gttctttcct tgcaagcaag tggatgactc aaatgaaaac
     6301 tcattgggtc ttgagaattg gactactaat cgcagctatt gggggcgctt ggttgtatct
     6361 tatgcctttc agaagtaact atttatggtt ttttgctata tcaacggtca tcggtatagg
     6421 cttaggtgtg accatgacta cctcgactgt cagtgcacaa aatagtgtat ctcgtgacca
     6481 gcttggtgta gcaacttctt ttaatacgtt agttcgaaca attggtcaaa cagtcatggt
     6541 ttctattttt ggtttattga taaatggggt aactaaacga gaacttgcag cagcaaattt
     6601 aacaaatgat gcagatatca tgaataaact ggtgaatcca caaacggcta aatatattga
     6661 tgcggatatt ttgttaccat taagaaaaat tctttatgat gggttacatt ccgtttatct
     6721 agttggcttg ttactcgtga tcattgcttt ggtcttaacg cttttcgttc atgaaaaaag
     6781 tggtaaaata aaacagtagt ttcggttaag atatgtacgt agccgaatca ttcaaattta
     6841 aagttattca aaaataactt cttactaagg aaggcgaaaa tatggcattt attcaagcaa
     6901 atatttattc gaatgtgtta gaaatggaag tcaatctaaa tgtgatacta ccgcaaacaa
     6961 caaaaaaggt gattggcacc agttcagagc aagagacaac ggatatacct gttttatatt
     7021 tacttcatgg aatgggtggc aatcatagtg tatgggaaag acgtacgtcg attgaacgtt
     7081 atgtagcaaa ctacggatta gctgtcatca tgccatctac tgatcttggc tggtacacag
     7141 atacagttta tgacatgaag tattggacat tcattgcaga agagctacct gagatttgtc
     7201 atgaattatt cccgcaatta acaagaaaaa gagaaaaaac atatgccgct ggattatcga
     7261 tgggcggata tggcgcgtta aaactaggcc ttgcaaaatc agaaaatttc gcagcagtaa
     7321 tctctctttc aggcgctgtt tctgtaggaa atcgaatgga tgatcttttg atggttcgta
     7381 aagcatcttt ttgggaaggg atctttggac cattggatca aatcgaaggt tcaaaaaatg
     7441 atccgcaata cttattgaaa gaactagtaa aaagcggaaa accagttcca agattttata
     7501 tggcttgtgg ggaatctgat tttctttatc aggcaaatca ggaaatggta tctgctatgc
     7561 aggaacaagg cttggcggtc acctttgaac aaggacctgg cgatcataac tggatcttct
     7621 gggaccaatg gattcaacgt gcattggcgt ggctaatgga agaaaaataa aaaaagagtc
     7681 ttaaaaagat agctcctata gagatcacaa acatagtgat ctctatagga gcttttttac
     7741 tgctcgtttt ctcttctttt attttgttgg cataagcctt caatataggt ttgtgcactc
     7801 gttagaaaga gtgctttttc attcaattct gcatgggact gcatactttc tttaaatccc
     7861 tctaattgat tttcaactac ataattacgc ataaatagaa ctgtttcttt caaaaattca
     7921 tttccgtcca taattttttg tcgaagctgc tgttcatgct gctgatccat taactgtcca
     7981 atcaataaat tgtggagaga ggcaatggcc tgatgacttt gggtgactga aaatccttca
     8041 cgaacaagta attggatcat ttgctccaaa tgttctaatc gactcggaaa agcgggcaca
     8101 gtattgattt ctaattctgc cccacaaggg aaacgtgtat aaaggtcata gtaattttcc
     8161 atatactgca gcaactgttc attccaaggc aaagttggat ctggaagcgc taattcttct
     8221 tccattgttt cagccatttt ttgcaagagc tgttgtttgt ttttgaaata ccaatataaa
     8281 gcaggtgctt gaacatgaat tctgtttgcg atgtttcgca tggaaagctg cgaaagagct
     8341 tgtgtttcag ccaggatatc aaaggcggcc tgaatgatga ttgcttgaga aagctttatt
     8401 tccattttat caacctcgtg tattgcatta tctttttaaa catggtaaat tgttagtcgg
     8461 tctaatttta cagtgttaaa ttagtcctgt caatttaaac aaaggggtgt aatcaacgaa
     8521 tgaaattaat cttacgacat gcgaaaaaat ataaagtagc tgtttttatt tccttgctgt
     8581 ccgtagcagt catggttgca gcagcattat ggcaacctaa gttactacag caagtattgg
     8641 aagctattat tacagaagat aatgatgaaa tgaaaacaat tggtatttac ctgatcagtc
     8701 tggctttgct aggattagcc gcaggtgtaa cgaacacgat tttctctgca aaagtagctc
     8761 aaggcgtaag tgcagatatc cgagaagaag catttcgaaa gatccagaca ttttcatttg
     8821 gaaatatcga acagttctct gctggaaatc ttgttgttcg tctaacaaat gatatcacgc
     8881 aaatccaaaa tttagtgatg atttctttgc aatcactttt tcgaattcca ttcttattta
     8941 ttggtgcctt cattttagct atgatcacga tgccgcaatt atggtggatc atcgtagcgc
     9001 ttatcgttac ggtatttttg attacggcac tttcatttac aaggatgggc aagcacttca
     9061 tgatcattca aaaactgatt gatcgtgtga atagcattgc aaaagaaaac ttactaggga
     9121 ttcgtgtagt gaaatctttt gtacaagaag aaaaccaatt gaagtcattc agcaaagtaa
     9181 gtgaggactt aacaaaacac aacatcatag tcggtacatt attttctgtg atgatcccgt
     9241 ccttcatgct ggctgccaat ttggctgtgg ttggggcgat cttttttgtg agcgatcttg
     9301 ttaaagatga tcctacgtta attggtggaa ttgcttcatt catgaactac ttgatgcaga
     9361 ttatgatggc aatcattatt ggcggaatga tgatgatgat gacttcaaga gcagccgtat
     9421 cgctgaagcg gattgcggaa attttagatg ctgaaccaga tcttacttat ttagacgttc
     9481 cagaacaaga gttgacgggt tctgtcactt ttgaccatgt ttcttttcgt tatcctggtg
     9541 aggatacaga tacgcttaaa gatatcagct tttctatcaa acctggagaa atggttggta
     9601 tcgtcggggc gactggtgcc gggaagtcaa cactagcaca attgatccct cgtctatttg
     9661 atccgacaga aggcaaagtt gaagtaggtg gtgtagatct tcgtcaagta aatgaaaaga
     9721 gtttaagaca aacggtttcc tttgttttgc aaaaagcaat tttattctca ggaacgattg
     9781 ctcaaaactt gcgccaaggg aaaaagaatg caacagaaaa agagatggaa catgcttctt
     9841 ctattgctca agcaaaagaa ttcatcgaaa agttatctga tcgttacgaa gcaccaatag
     9901 aagaacgtag cagtaacttt tctggtggtc aaaagcaacg tttatccatt actcgtggcg
     9961 ttatcggtaa tccaaagatc ttgatcctag atgacagtac gagtgcactg gatgctcgct
    10021 ctgaaaagct ggtccgagaa gcactcgatc gagatttgaa gggaacaacg acgattgtca
    10081 tcgcacaaaa aattgcttct gttgtcaaag cagaccgcat cttagtattg gatgaaggca
    10141 gattagtcgg cgaaggaacc catgaggaat tggtagctgg aaaccgtgtc tatcaggaaa
    10201 tctttgaaac tcaaaaagga acggaggagt aagtatgact gatctaataa aagcaagtaa
    10261 atttttctat cactatttga agcggtacaa actctccttt ttctttatct ttatcacagt
    10321 gatcattgct acctatctac aagtaaaggc acctcaatac atcggggaag cttttcaaga
    10381 attagctaat tacgtaggag gcttgatgca aggcgtagac gataaaagca aatttgtttc
    10441 tgtaatatgg aaactcttgc tgttttatgt actagctagt gcagccaact ttatctatag
    10501 tattttgttc acacaagtag tagggaagtc aacgaaccga atgcgtatcg gtctattcaa
    10561 caaattggaa aaattgacga tccgtttctt tgattctcat caagacggtg aaattttaag
    10621 tcgtgttacc agtgacttag ataatattca aaatagtttg aaccaagctt tgcttcaagt
    10681 catcacgaat gctgttttac ttgtcgggat cttgatcatg atgttccgcc aaaatgtaga
    10741 gttagcttgg gctacaattg cttccactcc agtagccgtt ttgatagctg tcttgattat
    10801 ccgcaaagca cggaaatatg tggatcttca gcaagatgaa gtggggaaac taaatggtta
    10861 tatggatgaa aaaatcagcg gacaacgtgt catcatcaca aatggcttac aagaagaaac
    10921 gattgaagga ttcttagagc ataatcaaag tgttcgcgaa gcaaccttca aaggacaagc
    10981 ttattctggt ctactatttc cgatgatgca aggaatgtca ctggtcaata cagcgatagt
    11041 tatctttttc ggcggctggt tagcattaaa tggtgatttg gaacgttcgg tggcattagg
    11101 cttagttgtg acattcgtcc agtattcaca acaatattac caaccactga tgcagatttc
    11161 atctggatac agcatgatcc aattagcgat tacaggggca agacgattga atgaaatatt
    11221 tgatgaaaaa gatgaagtca aaccagaaaa cggtaaagca tttgatggtg ttgaaaaagg
    11281 tcttgcgttg cagcacgtag attttggtta ttctccagaa aaactagtct tgaaagatgt
    11341 ctcgatcaat gtggacaaag gagaaatggt tgctcttgtt ggtccaacag gatcggggaa
    11401 aacgacgatc atgaatctgc taaaccgttt ttacgatgtc aatgatggtt cagtagtcat
    11461 cgatggaata gatatccgag attatgatct tgatgcatta cgtagacatg taggaatcgt
    11521 tttgcaagaa tctgtattgt tctccggaac gatcaaagac aatatcacat tcggcaaacc
    11581 ggacgcttca gatgaagaag tcatcaatgc agcaaaacaa gcgaatattc atgacttcat
    11641 catgacgtta gaaaaaggtt atgatacaga gatatccgaa gaaaataact tattcagtac
    11701 aggtcaaaag cagttgatca gtattgctcg aacgatcatc acaaatccat ctttattgat
    11761 tttggatgaa gcaacaagta atgtagacac ggtaacagaa gcaaggatcc aaaaagcaat
    11821 ggatgaagca atcaaaggaa gaaccagctt tgtgattgct caccggctga aaacaatctt
    11881 gaatgcagat agaatcgttg tcttgcgtga tggggaagtc attgaagaag gcagtcatca
    11941 cgagttatta gcagaaaatg gattttatgc ggaactttat cataatcaat ttgtatttga
    12001 ataaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca atcaaaaaca
    12061 gcttctcatg tttttgattt ggttggactt attaccgcag atataagctt ttgagcaccg
    12121 tgaataaaga ggttatgaaa tcaacgctct aacctgagta ttagagaaaa aaacgcaaaa
    12181 catatcattt atgttttgcg ttttttattt atcttttttt ctcaatacaa tataaaatag
    12241 gcggctggtt cttttggttg ataaaccgat aattcagcac attatatgtt tcctgtggca
    12301 gtttggaaca aaaagaatca accatatcca gttctttttc tccaccttca tgaccatagt
    12361 agacgacaag gatcaatcgt ccgcgaggca caagtcgttt caatatttct tccatagctg
    12421 tgcgtgtagt ctctggcata gtgatgattg ctttatcact ttttgggaga taccctagat
    12481 tgaaaatccc tgctttgaga ttttgttgtt cagctatcac ttctcctaac gtttcgtggc
    12541 cttgtagaaa caatgagaca cgttcttcta actgacgctg ttctagcctt tcacgagtag
    12601 catcgattgc ttgttgctgg atatcaaaac tatagacatg accagttttt ccgatgtgtt
    12661 ctgctagaaa aaccgtatcg tggccattgc ccatcgttgc atctacgacg tgatcccctg
    12721 gttgcaagat ttcttgcagt aatgtatggc tgaaatgcaa agcagtttgc aacatcagac
    12781 gctcaccttt ttccgtacat catattttcc ttggaagcta tctctgcgaa gaagttcttc
    12841 atcaattgca ttcaagactt cccatttttt caagctccac attggtccaa tcaaggaatc
    12901 ccacggcgca tcccctgtca aacgatggat gatgatctct tttggtatca tttccagctg
    12961 gtcgcaaatg acattgacat agtcaggacg gctcattaat tgaaggcgac cttcatgata
    13021 gtcccgcaac attctcgttt tgcgcatcag atgcatcaaa tggagcttga ttccttggat
    13081 atctgaatct aaaatggtgc gtctgacatt ttctttcatc atttctaaac tttcgcctgg
    13141 taatccgttg atcaaatgcg tacaaacacg gatattatgt ttgcgtaatt tagccacgcc
    13201 atctaaataa gtttgataat catgtgctcg gttgatcagt tttgaagttt cttcaaaagt
    13261 tgtttgcaat ccaagttcta cccataaata catccgctcg ttcagttcag caaggtaatc
    13321 aactacttca tctggcagac aatccggtcg tgtaccgact gataatccta cgacacctgg
    13381 taaattgact acttgctcaa atcgttcttt gattatttca actggcgcat gggtgtttgt
    13441 aaaattttgg aaataaacga tgtattgttg gacatgaggc catttttgat gcatcatgtc
    13501 gatttctttc tggaattgga ttggaagagg gtcttcaggt gcaacgatca tgtctcccga
    13561 accagataca ctgcaaaaag tacaaccgcc atgggcaaca gtcccatctc gattcggaca
    13621 gtcaaatcct ccatcgatcg gcaccttgaa aattttcccg ccaaactcat ttcgtaaagc
    13681 gtaattccaa gaatgatatc ttttatttcc ttctgaataa ggaaaagtca ttaaaagtcc
    13741 ttctttctgt aagagttatc tatttgtttt cttttttctc tatacactgg ataagtcagg
    13801 cacagccaag aaagactgag taagtagccg ccgatgatat cagtaggaaa atgaacgccc
    13861 agataaatgc ggcttatccc gacacttact ataataaaca tcatcagcaa ttgaagcaat
    13921 gtgcgtaagg ttcttttttg aacaaaagct ggaagcagaa atagtaaaga accgtaaaga
    13981 atcatactgc ctgctgcatg tccactaggg aaactgtaac ttgtttcaac tactaagtgc
    14041 gccaatgaag gacgctctcg catgaaaaat agtttgatca ccgggttgat gattccagca
    14101 attacaataa atccagaaac gaaccaaaat gtttctgccc tttttttctt ataccacatc
    14161 cataaagccg cacaagcaaa taaaatgatg acggtaagag gattaccgaa tttagtcata
    14221 aagagttggt agctgttcag atcaggataa gaagctctga caatgcctgt aaaccactga
    14281 tcgaaaccag aaagccacga cagatggaac tttacgatcc agcccaaaat gaaaaacagg
    14341 acaagaaaga agctaccagc cacctgaaat gatgttttat atttcataca attcctcctt
    14401 tgaagcaatg acttattata gcatttttgg tccagcgaaa aaaacggtaa agagataaaa
    14461 ccaatgtatg attcctcttt gaataaccaa tattcttata aaaaaaataa ctgtaatcgg
    14521 aaagacattt tttggtaatg caaatgaaat gttagggaaa taaaatcagt gtttttcgaa
    14581 atccttttaa acaagaacgt agacgtagga agaacttgaa aatgaacaga agttgttcat
    14641 tacggtaaat tacaatatga ttacaaaaaa caacaaagct cttaaataac ttttaataaa
    14701 tattaagaga agttataatt attaataata gataaattga agttggagga tagaagatgg
    14761 aaaacattgc aagaaaagaa cggagacgtt tagaacagac caaacgtttt cgaaaagcaa
    14821 aacgaggggc agcaatcgtg ggaactgcga tggtaggctg ttctgtcgct gccccattag
    14881 tacagccggt tcctgttcaa gcggatgaaa cacctgttca gttcggcgca agaatcaata
    14941 cagcggcatt tatcacagaa attgctactt atgctcaacc aatcgctcaa gccaacgatc
    15001 tatatgcatc agtgatgatc gcacaagcag ttgtagaaag cggttggggc agtagtgcgt
    15061 tatctcaagc accttattat aatctttttg ggataaaagg cagctatcaa ggacaaaccg
    15121 tctacatgga tacgcttgaa tttttaaata atcaatgggt aaataaaaag gagcctttcc
    15181 gacaatatcc ttcttttgct gaatctttca atgacaatgc ctatgtactt agaaatactt
    15241 catttggcaa cggttattac tatgccggta cttggaagag caacacgaaa tcctatacag
    15301 atgcgacagc ttgtttgaca gggcgttatg cgactgatcc tggttacgca ggcaaattaa
    15361 ataatatcat cgcaacttac ggattgaccc aatatgatac accagctagc ggaaacgcag
    15421 gcggtggagc gactacaggt ggcaataata ctggaagtgc aggtagtact ggcggttcaa
    15481 gcaattctgg taatacaaac tcaggttcag caacttcgac cacttatact gtccaatctg
    15541 gtgattctgt ttggggaatc tcaaataaat tcggtatcac aatggatcaa ttgatccaat
    15601 ggaacaacat ccaaaacaat ttcatttatc caggacaaaa actgacaatc aaaggtagcc
    15661 aggaaaatgg ctcttcgacg aataattccg gtaacaatac caattcttca ggaaatgctg
    15721 gtacatcaaa cggaggacaa acaactggtg cgaaatacac tgttcaatct ggtgattctg
    15781 tttggaagat ctcaaatgat cacggaatta cgatggctca attgattgaa tggaacaata
    15841 tcaagaacaa tttcgtttat ccaggacaac agctaatcgt atcaaatggc ggttcttcta
    15901 atgctggagc aaatactggc tcaaatactg ggaatacaag tggctctagt tcgtccagct
    15961 caagttcttc taatacagct tcaggaacga aatatacagt taaagccgga gactctgttt
    16021 ggagcgtctc aaataaatac ggaattacga tggctcagct aatccaatgg aacaatatcc
    16081 aaaataactt tatctaccca ggacaacaac tgactgtttc aaacagtgga tcaacaaaca
    16141 gcacgtcagg taatacaggt tcgtcaacca atacaaataa cagcagttca gttaatactg
    16201 gcagcagttc gtctcaaggc acttataccg tgaaagcagg tgaatctgtt tggagcgtgg
    16261 caaataaaaa cggcatcacg atggatcagt taatcgaatg gaataatatc aaaaacaact
    16321 tcatttatcc aggacagcaa ttgatcgtta aaggtggaac ttcggcaaat acaaatacag
    16381 gttcaactac ttctgcagcg aaaccaaata caccgaatac gtctgctacg acaagtacat
    16441 catccactgg taatacgatg tataccgtga aagcgggcga gtcagtttgg agcgtagcga
    16501 acaaacatca tatcacgatg gatcagttaa tcgaatggaa taatatcaaa aataatttca
    16561 tctatccagg acagcaagtg attgtgaaga aaggttcatc tcaatcaaca aaccaacaat
    16621 cagttactgg ttcgaaaacc tatactgtga aatctggaga atctgtttgg agtgttgctg
    16681 acagtcatgg aattacaatg gctcaactaa tcgaatggaa caatattaag aacaacttta
    16741 tttatccagg tcaaacgttg attgtagcta aataaaatag ctgttcgaag aaataacgat
    16801 tgaattccac aaaaatattt gttactataa ggctacttaa atatgacaac acttgaaaag
    16861 gaagtagtaa aaagactgaa gtttcagaga gcgcatggct ggtgagaatg tgcacggatg
    16921 cttttgaact cgcctttgag ttgcccttgc tgaatacccg agtaagcaag cggcggtgac
    16981 ggccgttaca acgttcgagg ccttttcact aaggtaaaat taggtggtac cgcgtagatt
    17041 acgtcctata aaaagagcaa tctttttata ggattttttt atttcttttg tgaagtttga
    17101 gaaaacaaag caaaaggaaa taaaaataaa cagattctgc cggttttttc gttatattta
    17161 taatagaaac aagtgttttc aatagaacag gaggacagca aatgagttac aatcataaag
    17221 aaattgagaa aaaatggcaa aaatactggg caaaaacgaa tactttcaac acccatgatg
    17281 atcctgaaaa gccaaaattt tacgcactgg atatgtttcc ctatccatcc ggtcaagggc
    17341 tacatgtagg gcatcctgaa ggttatacgg caacggatat cctttcacgt gtgaaaagaa
    17401 gtcaaggata caacgtgctg catccaatgg ggtgggatgc attcggtctg cctgctgaac
    17461 aatatgcgct agatacagga aacgatccag ctgagtttac ccaaaaaaat atcgaaacat
    17521 tccgacgcca aatcaattct ttaggattta gttatgactg gaatcgcgaa gtgaatacaa
    17581 ctgatccgga gtattataaa tggacacaat ggatctttac gaagctttat gaaaaaggtc
    17641 tagcttatga agcagaagta gcggtaaact gggtacctga attaggaaca gttatttcaa
    17701 atgaagaagt gatcgatggg aaaagtgaac gtggtggcta tgatgtagtc agaaaaccaa
    17761 tgcgccaatg gatgctgaaa atcactgctt atgctgatcg cttattggat gatctagaat
    17821 tagttgattg gccagaaagc attaaagaaa tgcaaagaaa ttggatcggt cgttcagtag
    17881 gagcaaatat tgaatttaaa gtggctggta cggataaatc ctataccgta tttacgacac
    17941 gaccagatac tctattcggt gcaacttact cggttcttgc acctgaacta gatcttgtta
    18001 gagaaattac gacccctgaa caaaaagctg ctgtagaagc ttatatcgaa gagacagcta
    18061 aaaaatcaga tctaaagaga acggatcttg caaaagaaaa aacaggagta tttactggtg
    18121 cttatgcaat caatccagtt aatggcaagg aaatcccaat ctggatcgcc gattatgtat
    18181 tatcttcata cggcacagga gccatcatgg ctgttccagc acatgatgaa cgtgactttg
    18241 aattcgctca aactttcgga ctagaaattc ttccagttat cgaaggtgga gatgttgaaa
    18301 aagctgctta cacagaagat ggtacgcaca tcaactctga atttctagac ggcatgaaca
    18361 aacaagaagc aatcgataaa atgaatgaat ggctagaaga aaatggcgta gggaaaaaag
    18421 aagtcagcta tcgcttacgt gactggctat tctcccgtca acgttactgg ggtgaaccaa
    18481 tcccaatcat ccattgggag gacggaacag tcacagctgt cccagaagaa gagctaccat
    18541 tacggttgcc taagacaaaa aacatcaagc caagcggtac tggagaatct ccattagcaa
    18601 atatcgagga gtgggtcaat gtcgttgatc ctgtaacagg taaaaaaggc cgtcgcgaaa
    18661 cgaatacaat gccacaatgg gcaggaagct catggtatta cctgcgttat atcgaccctc
    18721 ataacaaaaa agaactagca gattatgaaa agttgaagcg ttggctacca gttgacatct
    18781 acattggtgg agcagaacat gcggtacttc atttactata tgcacgattc tggcataaat
    18841 tcttatacga tatcggtgtt gttcctacaa aagaaccatt ccaaaaacta tacaatcaag
    18901 gaatgatctt aggcgaaaac aacgaaaaaa tgtcaaaatc tcgtggtaat gtagtcaatc
    18961 ctgatgatgt ggtcgaaact tacggtgcag atacactgcg catgtacgaa atgttcatgg
    19021 ggccattaga tgcttcgatt gcttggagcg aaaatggctt agaaggaagc cgtaaattct
    19081 tggatcgtgt atggcgtttg atcgttgatg agaacaataa aatgcgtgac cgtatcacta
    19141 cattgaatga tggcaagctt gataaagtgt atcatcaaac agtgaaaaaa gtgacagaag
    19201 actatgaaaa tcttcatttc aatacagcaa tctcccaatt gatggtcttt atcaatgaag
    19261 cctataaagt ggacgctctg ccttatgaat atatcgaagg atttgttcaa ctactcgcac
    19321 cgattgctcc tcacatgggt gaagaactat gggcaatcct tggaaatgat ggtggtattt
    19381 cttacgcacc atggccaaca tacgatgaag cagcacttgt tgaagacgaa gtagaagttg
    19441 ttttccaagt aaacggtaaa gttcgtgcga aaagcaatgt tccccgagac ttaggaaaag
    19501 atgagctaga aaaagcagca ttagccaatg aaatagtgca agaatatatt gaaggcaaaa
    19561 cagtcagaaa agttattgcg gtaccaaaca agttagtcaa tatcgttgct aattaattgg
    19621 ctctttgtca ataaggactg atgagttgtg caaaatcaaa tctgggccag aatgaacttc
    19681 attctgtctc agattttttg ttatttcaaa ttgattaatt gattgatcaa aaaggttttg
    19741 attttcatgt tctcaaacac gttcaatgtc tttttcttct gaatggtctt gtaagagatc
    19801 tctgtaatca tcaaattttt gtctattaat cttagtattt tttgatgtat aaactacctg
    19861 aaattgagag tacataccaa attcataaat tttttattag tgggactagt gattgtatgg
    19921 attttgacgc ataatgaatg aagtatgaga aaaggagaga caaatggatc aagaactatt
    19981 tattatcgaa gtattatcag ctaaacaaaa aattatcgag aaagcgaaaa aaagagcgaa
    20041 aagaataatg ttcaagcttt taagcttagt atttttctct ggtttgcttt ttttcggagc
    20101 tgtatcgttc ctatatcgcc atatgagtgc agtagtccgt tcagctgaag ggatgcaaaa
    20161 attaaataat ttgtatcact tttcgtgggg agttggcgga ataacaattg ttgtgcttat
    20221 tgtttttctt ctacttattt ggctccgtta ccatcaattg tcgcgtcatt tcgatcaatg
    20281 gcttagtcaa agagcacaac agtttttaca taataaacca acttctcaag atgatgacta
    20341 tctttatttt gagaagactt cgacgagtca agcttttcgg atacaaaaaa gtgattgcca
    20401 gttattgctg agatcacttg atgatttacc tatctactta ggcgaaagca aatcaaaatt
    20461 cggaatgaac acattccata tttttttact agaaaacgaa cccagatggg aaaaaagcca
    20521 gtcttcgcct ggtggatacc tgacaactaa gatgagtaca ttagtcactg gttttttcct
    20581 cattctttta gctggaacaa tctattatgg gagatacact cagccaaaaa tgtcttctca
    20641 tataaatgct gcttctcaca tgacaatgaa ctcaactaac gatacgcata tacaaaagac
    20701 aaatgaaggg ttgttagctg atcctaagca aaaggtaaat ttgacaacac aaaagataaa
    20761 tgatctcaaa cttgatacag agacgcactc tctctatatg acaacagata gtggtgctaa
    20821 ttgggtattt gtgccgattg attcgcaatg gttgcgtttt ggcgaataca ccctgacaac
    20881 ggggctgatt cctgaaggtt attggatgga caacacatat gatatttccg ccgatttctc
    20941 atggtttatc tattcttctg gggatgatgt acgtctgctg acttcaagag ataatggtaa
    21001 aacatggcaa acgtctatta tcacccaatc agttcagccg atacgttatc gaaaagcggt
    21061 attttatggc aacgatggtc tatttatctg tacatcagcc agtgggatgt ctgcagaaac
    21121 tttgtacatt tacactacta tggatggcgg aatcacttgg ataaaaagtg gaagtacaat
    21181 gatcaatcag ccagtacaaa atgtcagctt cgttacaact tctttaggtt ttgtttcgac
    21241 acgtgaaagt ttgttttata caaacaacgg aggacgctca ttcaaagaat cagttctgtc
    21301 gattccggaa aactatcaga caggggggat agatcttttt caatcaccaa atgaagttac
    21361 tcaaataagc gcgaatacgt tagagacaaa attttatttg ttgaaaaaag gaccaatcga
    21421 tgcagggaaa atgtttgcct gcctcttccg ttcaactgat aacggggaaa catggcaatt
    21481 cgttcagcaa ctatcgcaag tagaacaagg agattaaaga agagagattg tgagcctgac
    21541 gttcagcacc gagtcacaag aaacaaattc aaaaaacagc ttctcatatt tgttgaatag
    21601 tgtaactcgt gattcggagt tgacaagagc aacaagaagc gattttgaag tatagcttct
    21661 cgtattttac aagatcgtgt cttattgccg ataggttgtc tcttgaacac cgtttattcg
    21721 taaaaaggga ttgtgacaag tcttttgtca ctacttaccg aacgaaactg aaggagctat
    21781 ccaaaaagta tcattagtat tattctttgt gtttctagtg gcaccactag tacatttaaa
    21841 aaagctatcg atcatggact aatcatacat gatcgatagc tttttagata atgtttgtta
    21901 cgctatctga aattgactat tataaagact ggcataaagc gttggtgatt ggagtagttc
    21961 agaatgtgtt cctttttcga cgatatcgcc atttttcatt acaaggatca tgtctgcatt
    22021 ttcaatcgtt gacaaacggt gtgcaatcac aaaacttgtc ctatcttttg tcacagcttc
    22081 cattgcttgc tgaatcaatg cttctgttcg tgtatctacg ctagaagttg cttcatctaa
    22141 aataacgaca ggaggattcg ctagaatgac tcgagcaatt gtcaataatt gttgctgtcc
    22201 ttgagataac gtgccgtttt cgctagaaat gatcgtatca tatccgtctg gcagtgttcg
    22261 aataaagtga tcacattgtg cgatttcagc cgcttcgata atttgttctc ttgtggcatc
    22321 cattttcccg taggcgatat tatcagctac cgttccttca aacagccagg tattttgtaa
    22381 aaccatccca aaagaacgac gtagttctcc acgtgaaagc ttggtaatat caagaccgtc
    22441 gaattcgatc tttcctttat tcagttcata aaaacgcatc agcagattga ctaaagtcgt
    22501 ttttcctgca ccagttggac caacgatcgc taccgtttgt tttggcttta ctgaaaaatc
    22561 gacttgattc atcaatattt tatctgatgt gtaaccaaac tgtacatttt tgaaagctat
    22621 ttttcctttc ggttgttcta aaagtcttgc atctggaagg tctggaattt catctgtttc
    22681 atctaatatc tcaaatatcc tatcgattgc tgccattgcg gattggatcg agttgatcac
    22741 ataagaagcg gtagaaattg gttcagaaat ctgattgatg tactgaagat aagcttgtaa
    22801 gaagccgatc gtcacaccac ctgatagaac aagcatagca ccgaataccg cacttatgat
    22861 gaaggcgagt tgattaatga aacgaatggc cggatagata gcaaagttca taaattgagc
    22921 acgtttaaaa gcgttgtatt gctttttatt tacttcgtgt atagcagctt ctgcatgagc
    22981 ttgctgattg aatgttttga ccactagatt accagctaag atttcttcca ttcgattatt
    23041 taaaacaccc agttcatact gattctgttc agaaatctgt ttgtttttgt tcgcaatctt
    23101 agttgtaaca attgaactgc caacgatcag caataagacg agcaaagtaa gcttgctttc
    23161 gatataaaac agcatcagac cagcagcagc tattgttacg acagaagtaa aaaattgatt
    23221 gatacctgtc aataacactt gagaaagttg attgagaccg gtagttgtcc gactaatgat
    23281 gtcacctact tgatgattgt caaaaaacga catcggtaat gttttgaatt tttttgtgat
    23341 ttcttttcgc accctcaatg tgatcttttc gcttaacgct gccattgtcc gttcctgaat
    23401 aaatgaagtg atgctgctta agactgaaca gccgatcagc aataagacag ggaacaacaa
    23461 tgcttgtttg atatcactga aagaaagctg tccaatacct gtttttctta tttctgataa
    23521 gagatcatcg atcccgattc ccatgatcaa gggcatggct acgactaata ggttcccgaa
    23581 taaactgtat agaaacaatc cgtaaaaata tttatgttca ggtttgatca ttttccaaaa
    23641 acgtatcaaa gcacgcgtgt tcaacgtttt tttcttttcc atcgtatccc ttctttctat
    23701 attccttgtg attttgcaaa ctcctgataa gtttggcaat tagacagtag ttcttcatga
    23761 gtaccttgtc caacgatttt tccttcatct aaaacaatga tgttatctgc ttgttggatt
    23821 gtactgaggc gttgagcaac aatcacgaga gttttctccg acatgtgttt atgaagggct
    23881 gctcttaatg ctgcatctgt tttatagtct aaagcggaga aactatcatc gaatacatag
    23941 atctctgctg gtttgatcaa cgctcgagca atacatatcc gctgtttctg gccccctgaa
    24001 taattagaac cgccttgagc aacaaagctg tccaatccgt caggaagggt gtccactagt
    24061 tcttctaatt gagcgatctt gatcgcttta gacaactctt ctgttgaggc atattcattt
    24121 cccatctgca agttgcttaa aattgtcccg ctgaataaga aagccttctg agggacataa
    24181 ctgatgtggg agcggatctg ttcttgagtc atttgagtaa gaggaatacc accaagggtt
    24241 acttttccta tcgtagggtc actaagtctc aagagaagtt tcgccaaact actttttcca
    24301 gatccggtcc cgccaacgat cgctgttgtt tttcctttag gtattgtgaa atcaatatct
    24361 tctagaactg gatcagagat ttcagtatat ttaaatgtga catgttcaaa agcagctgca
    24421 tttgtctgat caaaggttgg ttcagctact gttttgatcg tctcttcctc aatttcgatc
    24481 tctgcttgca gtatttcttc tatccgtcgt aatgaggcaa acgatttagg gataatgacc
    24541 aataccatag ctgcgatcat caagtaagca agtgtcagca tagagtattc gactacagca
    24601 gtcacgacac cgatttgtaa tgctccttct gctacaagat ttccgccaaa ccacaaaata
    24661 gaagcataaa cgattcccat gagcaaaaaa gcaattggag tgacccaagc aaatattcgg
    24721 ttcactttga tcatttgatt agcatagtca gcaaagctct tattcgttcg cttttcttca
    24781 tatgattgat tatcaaaggc acggatcata ttaatcccag taaagaattc tctcaatgtg
    24841 atggtgatcc gatccatttt tggctggata ctcaaagata aaggtgcccc tcgtttcatc
    24901 agtcgataaa caatccatac ataaaaaaca acagagatca atgtaaccca tgttagtaat
    24961 agagaataac gaaacatcat gagaatggtg aaaacgcaaa taaccggtgc tggtaagatc
    25021 atttggcaaa ataaaacaat catttgctgg acattatcga catcattcgt cattcttgtc
    25081 aaaagagatc cagaactaaa acgactgata tccttcattg acagctgttg gaatttacgg
    25141 aaatatttct cccgcgtttg gaatccaaac ctagcggaag ccaatgctga aaagtaacta
    25201 gaggcaactg cagcgattgt tcctagaaac gctacaagca gcatttcaac tccaattttt
    25261 tttataactg aggcttcgcc acttgctata cctgtgtcga tcagctttgc tactaatttt
    25321 ggtacaccta atgtaccggc gatttgtaaa cagatcatca caaaggtaag gagagcgagt
    25381 cctttgtttt tctgtaagaa tgtttttaag atagacatat tattcctcct tttttcctga
    25441 tacttttcga ttctatacct tcaagtaact tgaaggtcaa tagaaaaaag aataaactga
    25501 aaaagaggtg agaaaaaatg aaaaaaatgt atacgattgg ccaagtatcg gaactatttg
    25561 atttgccaaa atcaacgatc cgctattggg atgaacaagg attaatccgt tcaagtagac
    25621 aagaagagaa tgattatcga ttgtttgata tcgatgatat ttttatgctt tatgatatcg
    25681 ttttttatag aaaattagat ataccgatca aacagatgaa gaatttatat ggaaaaacat
    25741 tgacggagtt atatgagaca cttgatgaaa cagaaagaag aatccataaa gaactggtgg
    25801 tgatgaaaca aaaacaaaaa gagataaggg agcgaaaaaa acagttgaaa ttgatgatcg
    25861 atacaaacga ggaagaattt cctgtagaag agatcccttt tgactgtatg atctcaacgg
    25921 agtttgaaga tattgtggaa atcaaaaaat ttctacctaa ctattcaagc tttgggatga
    25981 tgagtatgcc agcttccagt tcacagacga tgtatggatt ttttatcgac cctagtgaag
    26041 tacatttatt tactcaagac gtgatttggg agaaaaaaga tacggctgtt tatcgtcgat
    26101 tcttattgaa atccgagatg aaccatgcgg aacgtaataa tattttagaa atcagagaaa
    26161 gaatgtatga aaaaggatgg aaaacaggag aagtgatcgg ccagtatcta ttgacgaata
    26221 cggatgaaaa caatatcaga actgaatatt atcatgcatg gatagaaatg aaaaaatagg
    26281 aaatatgtgt ttaagataac tcgaaaatga tggttgtttt agttataaag aaaaattttc
    26341 tggttcgatt acgggtttaa tgtgggggat tattagtcgt ggatagatag aattttagtt
    26401 ttttagagga aatatgtctt taaaaatgga atggttgcaa ttcttagcga tatatacgat
    26461 gtaattgtcc tcttaaaaaa gtatggaaaa ataattgtat tgggtgttat gtataattca
    26521 tggtgaactg agactaatga gaagaaaaat tcatattgtt atgagtgaga ttgtatcaga
    26581 tgagaatgat aaattaaata ggtgcataaa agcattacac ttaaaata
//
LOCUS       DAZKCY010000029        24755 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000029 DAZKCY010000000
VERSION     DAZKCY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.029"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..571
                     /locus_tag="ACG8XC_001100"
     CDS             <1..571
                     /locus_tag="ACG8XC_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFP9190813.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            575..1216
                     /locus_tag="ACG8XC_001101"
     CDS             575..1216
                     /locus_tag="ACG8XC_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9190814.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1213..2130
                     /locus_tag="ACG8XC_001102"
     CDS             1213..2130
                     /locus_tag="ACG8XC_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9190815.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2127..2798
                     /locus_tag="ACG8XC_001103"
     CDS             2127..2798
                     /locus_tag="ACG8XC_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9190816.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3339..3827)
                     /locus_tag="ACG8XC_001104"
     CDS             complement(3339..3827)
                     /locus_tag="ACG8XC_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFP9190817.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4028..4294)
                     /locus_tag="ACG8XC_001105"
     CDS             complement(4028..4294)
                     /locus_tag="ACG8XC_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP9190818.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4294..4557)
                     /locus_tag="ACG8XC_001106"
     CDS             complement(4294..4557)
                     /locus_tag="ACG8XC_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9190819.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5078..5218
                     /locus_tag="ACG8XC_001107"
                     /pseudo
     CDS             5078..5218
                     /locus_tag="ACG8XC_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5360..7183)
                     /locus_tag="ACG8XC_001108"
     CDS             complement(5360..7183)
                     /locus_tag="ACG8XC_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9190820.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7781..10486)
                     /locus_tag="ACG8XC_001109"
     CDS             complement(7781..10486)
                     /locus_tag="ACG8XC_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFP9190821.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10792..11739)
                     /locus_tag="ACG8XC_001110"
     CDS             complement(10792..11739)
                     /locus_tag="ACG8XC_001110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFP9190822.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11929..12198
                     /gene="rpsN"
                     /locus_tag="ACG8XC_001111"
     CDS             11929..12198
                     /gene="rpsN"
                     /locus_tag="ACG8XC_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9190823.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12374..12736)
                     /locus_tag="ACG8XC_001112"
     CDS             complement(12374..12736)
                     /locus_tag="ACG8XC_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9190824.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12747..13868)
                     /gene="alr"
                     /locus_tag="ACG8XC_001113"
     CDS             complement(12747..13868)
                     /gene="alr"
                     /locus_tag="ACG8XC_001113"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP9190825.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13884..14234)
                     /gene="acpS"
                     /locus_tag="ACG8XC_001114"
     CDS             complement(13884..14234)
                     /gene="acpS"
                     /locus_tag="ACG8XC_001114"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP9190826.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14364..15875)
                     /locus_tag="ACG8XC_001115"
     CDS             complement(14364..15875)
                     /locus_tag="ACG8XC_001115"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9190827.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16197..17549)
                     /gene="murF"
                     /locus_tag="ACG8XC_001116"
     CDS             complement(16197..17549)
                     /gene="murF"
                     /locus_tag="ACG8XC_001116"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP9190828.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17702..18778)
                     /locus_tag="ACG8XC_001117"
     CDS             complement(17702..18778)
                     /locus_tag="ACG8XC_001117"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9190829.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18974..20719
                     /locus_tag="ACG8XC_001118"
     CDS             18974..20719
                     /locus_tag="ACG8XC_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFP9190830.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(20999..21436)
                     /locus_tag="ACG8XC_001119"
     CDS             complement(20999..21436)
                     /locus_tag="ACG8XC_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP9190831.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21420..22793)
                     /locus_tag="ACG8XC_001120"
     CDS             complement(21420..22793)
                     /locus_tag="ACG8XC_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190832.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23115..23603)
                     /locus_tag="ACG8XC_001121"
     CDS             complement(23115..23603)
                     /locus_tag="ACG8XC_001121"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9190833.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23625..24227)
                     /locus_tag="ACG8XC_001122"
     CDS             complement(23625..24227)
                     /locus_tag="ACG8XC_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9190834.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            24326..>24755
                     /locus_tag="ACG8XC_001123"
     CDS             24326..>24755
                     /locus_tag="ACG8XC_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9190835.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT         7146 a         5227 c         4584 g         7798 t
ORIGIN      
        1 agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc caatttgata caccagaaaa
       61 tattttacgt catcctgcaa acgatttcgt agaagagttg attggagaag atcgcctact
      121 acaagccaag ccaaatgcga caagagtcgg cgaagttatg ctaaatacag caatcacgat
      181 tacaccggaa aaatcattac aagaagcaat caaactgatg agggaaaaac gtgtagatac
      241 gttgttagtt accgataatt cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa
      301 taaaaaacga ggaaaagtat ccagtgtcgg cgacattttg aataaacatg ttttttacgt
      361 aaaagaatcc gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata
      421 cgtgccggtc gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg catcattagt
      481 ggatatcgtc tacgatgtga tttggggaga agatcccgca actactgatg ttgcagagtc
      541 agctactcct gaagaatcta aggaggtctg acctatgcag cagttttttc atcagcacgg
      601 ttcagaaatt ctctcaaaag gttttgaaca tctttatatt tcaggacttg ctttgatcat
      661 tggtatcatc attgctgtac cgcttggtgt cttactgaca cgttttccaa aagctgccac
      721 gattgtgatt ggcttaacca gtgccttgca gacaatccct tctcttgctt tattggcatt
      781 gatgatcccg ctatttggcg taggtaaatt acctgcgatt atcgcactct ttatttattc
      841 attattgccg attttacgta atacgtacat tgggatgaaa aatgtcgatt ggaattatcg
      901 ggatgtggca aaaggcatgg ggatgactga gttccaatcc attttttccg ttgaattgcc
      961 cattgctatg ccaacgatca tggctggtat tcgtttagct gccgtttacg tgattgcatg
     1021 ggctacactt gcctcttata tcggcgctgg tggtttaggc gatttgattt ttagtggact
     1081 gaataattac caaccagatc tcattttggc aggtacgatc cctgtaacaa ttttagcact
     1141 attggctgat tggcttctag gtctcttgga acaccggctg acaccgatcg cacttagaga
     1201 ggagaatgaa gaatgaaacg tatactaaaa atgaccatgg ttcttttctc tcttcttata
     1261 cttgcgagtt gttccttccc tggtcttgcc agcaatacag atgaagacac catttcgatc
     1321 acaggtggaa tcacttcaga agcacagatc ttagcaagtc tagttgccgg aatggttgaa
     1381 cattatacag ataaaaatac cgcaatcatc aacaatctgg ctactacaac tatcaaccat
     1441 caagcgatga tgaacggaga tgcttcaatt tcagcggctc gctacacagg aacagattta
     1501 acgactacat tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa
     1561 gatgaatttg agaaaagata cggtcaaacg tggttcccgt cctatggttt tgaaaatacg
     1621 tatgtctttc ttgttcgaaa agacaccgca caaaaatacc atctatctaa agtcagcgat
     1681 ctgaaaaatg tagcggatga attagtagct ggtgtagata cttcttggat caatcgtaaa
     1741 ggagacggtt atgacggctt ccaagaaact tatggcttct ccttcaattc gatattacct
     1801 atgcagattg gacttgttta tgacgcagtc gaagcaggaa aaatggatat tgttcttggc
     1861 tactctacgg atggacggat cgccagttat gatctagtca tgttggaaga tgatcgtcaa
     1921 ttcttcccac catatgatgc tgctccagtt gtagacaaca agatattagc tgatacgcct
     1981 catttaaaag aagccttaaa taagttaggt cataccatca gtacggaaaa aatgcaggaa
     2041 ttgaactacg aagcagataa taatctagtg gaaccatcag tagtagcaca acgtttctta
     2101 caagaaaacc actattttga agggaagtga agcaattgga taaaatgaat gtctttcaac
     2161 aatttttcta ttatttccag gaaaatggct cctatatctt tgcacaattt atccgtcact
     2221 tcctgatttc tatctatggt gtcctctttg cagcagttgt cgggattccg gttgggatca
     2281 tgatttctag gagacgtaaa ttagcaaact gggtgattcg tttggcgaat atcatccaga
     2341 cgatcccttc tctagccatg atctccattt taatcattgg attaggcctg ggagtcaatg
     2401 tcgtgattgt tactgtattt ttgtattccc tcttaccgat catcaagaat acgtataccg
     2461 ggatgatcca agtagacaaa aacatccttg atgtcggcaa aggcatgggg atgactgcta
     2521 gacaacgact attcatggta gagttaccac tctctgtttc tgttatcatg gcaggcataa
     2581 gaaacgcgtt agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg
     2641 gtcttggaga tatcattatc cgaggaacca atgcaacaga gggcacatcg attattttag
     2701 caggtgcatt gccaactgca ttaatggcga tcatcacgga ctggctttta ggtattttgg
     2761 aaagacggct agatccagcc agccgtacat cacgctgatg ttattgtaaa aaacaaaagc
     2821 ttctgcgacc atttgttggt cgcaaaggct tttttagttc tgtcattttt aaggcaataa
     2881 tccactctga ttcttccctc cttatgggca aagagttgct tttcttagct atttatccat
     2941 tccgtatact ccaacttcat actgccaacg ggttcatatc tgcatccacc gactaagttt
     3001 ttttcttatc cgctaaggca ttttgccaat ccattttccc atcactcgtc atggaattca
     3061 taccacatga tctctaaata ctcgtctgct tgttctttga attgggcatc aattcttaca
     3121 tcctttggcg tggctttcat ttaagccttt tccatcagtc ctcgattgtt cgatgccttc
     3181 agctgcatca tcaaaactgg tgactgccgt atcggtaact tcgatcatct tttatacaat
     3241 cgggaacttg ccatcctcat ccttggctaa aaacatatct agccattcca gcttctcgcc
     3301 atgggaggtt tgcggttcct aaaaccggta cacgattctt atggactgca tccgtcgcaa
     3361 ctagacttgg cgatacgatg atccttcacc agaagagcac ccccgtaaac caattgatcg
     3421 atatattgcc aataagaaaa gacattggcg taaaagatat cgatccctcg tgatgcattg
     3481 ccgttgatac tgctattcat ggccgaaagc gccacgactt taacttcttc cgtttgagtg
     3541 acattcatgt tcggcaatgt cgctttggat acccgctctg ctaaaggaac gacactggta
     3601 ttgtatttcg tatctgtatc ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa
     3661 taagacgact ccggttgatt gtccattgct tttttcaaca tacttttggc tgtttctcga
     3721 taaacgacat ttccaattgt cttcgcctca ctttggcgat aatcgatggt cgttgccact
     3781 gccaacacta acaacgatgc tccaatttta ctgattttcc ttgtcacact tcttcctcct
     3841 tgttttacat aaacccgtca acattccatt cctaaactca aacaatgacc cttcaaaggt
     3901 ggagatatag gaacaaatta aagaaatcat gcaataaatc caagaaggtt ggttttagaa
     3961 caaaaaaaga gtctcctttt tggggagact ctttgttaac tatctaaaaa ggttggctct
     4021 tctcacttca ctcataatgt gtccaggcag aataaatttc tacaacttta ttttcctcat
     4081 caactttgta aaccactcga tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg
     4141 taggtctcag cttttcaaaa gactgcgtgg gcatatatgg atcttccttc aatgtctgaa
     4201 tcacttcttc aaattgtttc tttaaattcg tttgcttgat ttttcttaaa tccactttcg
     4261 cagagttttt gattgcgact gtatagttac tcatcaaaga gcttcccaat caatatcatc
     4321 gatattcgta aagctactgt cgtctttttc gcggtctcgt actttatcta aagtacctgt
     4381 ttgctcaaga aaaagggttt cttgaatcgc tctccagtct tccagcccaa tcaatacagc
     4441 attgtttcca ctacgatcac taataatctc gatttcagta tgattttcat ttacctcttt
     4501 caataactga taaaaatttt tacgagcagt gctaggattc atcgttgtat tagccattgt
     4561 ctctcctcct ttttctgata taattatagc gtacttgatt aagtacgtca aaaaaataca
     4621 ataaagcact taaattcagc aaacttttcc tcttttttac atagagaaaa agaccccttt
     4681 ttagggagga tctttgtcca cgctcacttc ttactccttt acgactagcg gtagccaaac
     4741 gtaaagtaat tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt
     4801 ctatgcaaca acaccaatac acgaaggttc ctcgttgcag tgtgacttga ttgatatctt
     4861 gaataacctg aggatacgca cgaaagattt gtaacggtta ttcaaaactg attttggccg
     4921 tcaagtcttc tactgcctca aaaatgattg cctctttcgt ctcctgtcat tcatgttcac
     4981 tatcgatttg atacttgctt agaccgttgg gtttgtgaat caccaattaa aaatggcact
     5041 gagttatggt aacaaaaaat ggtcacttga cgaggctgag attcctactt atgaagacac
     5101 atggcactta agagtcgagg taaaggaaga agctgaaaat ataggaggct gtacattatt
     5161 tggcaaagga ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt acagctaaat
     5221 aggaagaata ataacatttc atacgctgtt cccatcttta atggcttata gagggcacct
     5281 ttagctgaga ataagacaaa acagaccctc tagcttcaat taagagctag agggtctgtt
     5341 tttgatttta aaacttggtt tatttcttca aatggtagct ataggtgctg acaacgatat
     5401 tttcacgcca agacaatcgc aatcttaacc gcaaacttgt ttggttatgg agaatattct
     5461 gccagcgtct gtttggtaca aattgaggaa tcaacacagt agttgtatag ttttgtttcg
     5521 ctgcattttt acttacgata tctacataac ggataattgg attttcaatc gagcgataag
     5581 aagagtggac gattgaaaaa cggacatctg ggaaatgctt tttaaattct gcttcgattt
     5641 ctttttcttt ttcgacatct tcgtctagcg aaacatgcat cgcaacgaca taatcaccga
     5701 tcgaacgtgc gtaattcaac gcaccgatat ttacccgtgt cacattcccg actaatacaa
     5761 gaacggtatt cccgtcataa tcatgtagtt ttgcatcatt ttctaaacga agctgttctg
     5821 ctactttttt ataatgatca tggatcttat aaaaaatgaa cataacgatt ggcataatga
     5881 tgaagaaagg ccaaatgtcc cctaaccgat agacaaacaa aatcgcaatg attgcatatg
     5941 agatgaacgc acccgtaata ttggcaatcg atttgcttaa ccatcttttt ccttcttttt
     6001 tccatttgac gaccatccct gtttgagata aagcaaatgg aatgaacacg ccgatagagt
     6061 ataacggaat caagcgttct gttgagccat ggaaaataaa cagcaatacg atcgaaccta
     6121 gcgctagtgt gataatccca ttggaatagc ctagtcgatc cccacgatct tgatacatgt
     6181 gaggcatgaa tttatctttt gccagattgt atgcaagaac tggaaaagca gaaaaaccgg
     6241 tgttagcagc aactgctaaa atcagcgctg tagcaaattg aaggacgtaa tacaaaatac
     6301 catgtccaaa gacagcttta ccaatttgag acaaaacagt gacttctttt tcaggaacga
     6361 tcccatacca atagttgata aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca
     6421 tcatcgtcaa tgtagcagcg gcattttttg ctcttggctt cttaaagaat ggtacagcat
     6481 tactaatcgc ctccacccca gttaaagagg aagaaccaga agaaaatgct cgtaaaatca
     6541 aagcgataga aacacccggc acagccgctc ctggaagtgc agtagcttgc aatggcacag
     6601 cacctgtcac gatattatat aaaccaacaa cgatcagtaa cgagatcaca aggataaatg
     6661 tgtacaccgg aaataatagg aacgatgcag attccctcag cccacgaaga ttcagcatca
     6721 tcagtagcaa aacgattgtc acagaaatag cgacttgatg accgtagagt gctggcaccg
     6781 ctgatgtgat cgcttcagca cctgctgaga cagatactgc tactgtcaac atgtagtcga
     6841 tcaataaaga gccaccggaa atcaagccag cattctttcc aagattctca ctgctgacga
     6901 catatgctcc cccgccatga ggataagcat ggatgatttg acgatatgat agcgttaatg
     6961 aaatcaataa aataataaca aaagcggcaa tgggtagcga ataccagatg gctgcagcag
     7021 acaaagcgac taaaacgaca acgatttgtt ccgtaccata tgcaatagat gataacgcat
     7081 cagacgacaa taatgccaaa gcagcaaatc gggttaattt gtgctcatca ttttcagcag
     7141 attttaaagg cttacctact aacaaccttt ttaagtaatc caatttctaa actcctttac
     7201 acacaatttt acttcaagtt ttatagtcga aaatttatag taaacaagat gttatagtac
     7261 gctcactgac cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa acagaaaaat
     7321 atcaagcgct ttttcaaacg ctttaataca gcaaaaatct aacatttttt tatgggcttt
     7381 ttctttgaat tctataataa aaggatagaa cttgacttag aaaatcaagt tctatccttt
     7441 ttgatatctt aacttttgga agagagatgt aaggctgctt tctacccatc attcttacta
     7501 ctcgaagcat agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct
     7561 gcggtaataa gctcaaccaa attaaaaaca taagaagctg tttttatttg gtcgttctta
     7621 ttacttgatg cagagcagct tttttacaac ctcttggtac acggtgttca atcagctgac
     7681 cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac tatttttcca tttttttctc
     7741 taatactcag gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa atatggctct
     7801 catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag attgccgttc
     7861 ctacgattcc aaagacaaca aacaaaccga tcatgatcca gattgctttc gtagcatttg
     7921 gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta aatggcaaga
     7981 acggattgat catttggaag aatttccctg agacttggat cggatagttc ccgcctccgc
     8041 cagagatcga cagtactaag atgataatag cgatcccttt accgacatta tcgaataatg
     8101 cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca aatgacacgc
     8161 tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat gtaacgatca
     8221 agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt
     8281 tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca acacttgaaa
     8341 gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta gctgaaccgt
     8401 tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta aagaagtcac
     8461 tttcttttgt tgcgtctagt ttcaataatt tgatgacttc gcctagatcg acttcttgtt
     8521 cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt ggccagtcat
     8581 tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt ttttcattta
     8641 ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt tggataaaat
     8701 cagcggccaa tcctaatttt tgctgaatga tcggtaattc attgttgtat aggtctgccc
     8761 cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta ttggcatcat
     8821 ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt gccactgcat
     8881 tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc
     8941 tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga gaagtgacag
     9001 tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta ttaacaacgg
     9061 catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga acagctgtgt
     9121 tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga tttagctgat
     9181 caataattgg ctgtaatttc gtatttccag cacttgtttg caatttagtc aggaactcga
     9241 ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg ttcaatacat
     9301 tgctgatgtt tttcttttca tcatcagtca gcttaccatc ttcaatagct gttcttacag
     9361 tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgacttcaat gtaacatcta
     9421 cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat tttgtttggt
     9481 ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata tcttgtactt
     9541 gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt gtctcttgga
     9601 tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc ggcattttgg atctctggga
     9661 ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc attttgtcat
     9721 tcaatgcaac gactttttca cccatctcat ctactttagg gatgtaatcc acgaattcat
     9781 ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg aggacttgtt
     9841 ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata tctttaactt
     9901 tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat actttcaata
     9961 aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc gaacttgctc
    10021 ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta tattcgattt
    10081 ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa tcctttggca
    10141 agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt tcttttgaat
    10201 caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact tgctccccaa
    10261 tggccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca gcaattggca
    10321 gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac gcatatagcg
    10381 aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt
    10441 tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt ctcccccttc
    10501 ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat cggctttttt
    10561 caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc tcttacatat
    10621 tgagaaataa ttaaggatta cagcctttac tttatcatga aataaaaggg aaagataatt
    10681 ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca ttttttgtct
    10741 ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt cttatttttc
    10801 gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat gtgcttggat
    10861 gacatgctta agtgaagcag acagattcat tgaaataaaa acatgaaagg ctcctttttg
    10921 gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc ttggaacaaa
    10981 cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt catttaatgc
    11041 aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga tacttccttt
    11101 cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat ttgttttatc
    11161 ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt tactggatgc
    11221 actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg acccgaccgc
    11281 acctgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca aatggttgat
    11341 tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc aactcatttt
    11401 atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt aagtcccgcc
    11461 gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg aaccaatctc
    11521 tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg ttcgtatctc
    11581 tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg cttttatttc
    11641 tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt caaaaacatc
    11701 ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct ccctttttct
    11761 ttaagtttac gtttatgtct ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg
    11821 attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta tctaaaagcg
    11881 taaagattac gatttacgct aataaaaaat aattggagga atttgttgat ggctaaaaaa
    11941 tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc tgctttacgc
    12001 caggaattaa aagcgcaaaa agattacgaa ggcttgcgta aacttccttt agatgctcgt
    12061 ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata catgcgtaaa
    12121 ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat tccaggagta
    12181 aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg cgcatggacc
    12241 actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc tggtactaga
    12301 agcagacgta tcttttttta cgcacagctg gtcctttttt atatgtaaag gctgcatggt
    12361 tacttcgcta tatttattta gtaacaggag tcagtgcttc gatacccaca ctaattttca
    12421 atgctcggtc aacttctgcc ataatgggtc gatccaaatg gcagaccttt tctttcaatc
    12481 ttgatttatc gatcgttctg atctgctcca acaggataac ggagtctctt tctattcctg
    12541 ttccagtagc tttgatccca atgtgagtcg gaagcttagg tttagccatt tttgccgtta
    12601 tggccgcaac aatgatcgtc gggctaaaat ggttacctag attattttga ataaccaaaa
    12661 ctgggcgtgt tccgccttgc tccgatccca cgactggaga aagatcagca aaataaatat
    12721 ctcctctttt taccatagga tcctccttat tcttgatatt ctcttggtat ccgctgtcct
    12781 aatccgcaag ccacttcata gtggatcgta cctaattgat ctgctatatc ctgcatagtg
    12841 atttctttgt ctgcattttt gccgataagc gtcacttttg ttccgacagg aaattcttga
    12901 ggcaatcgga tcatcagttg atccatacat acacgcccaa tcgtttcaca gtagttccct
    12961 tctaccaata gagaaaaacc ctgcatcttt cgtggccagc catctgcata accgatcggt
    13021 atcgttccga tccactctgc ttcaggagtg atatatgttt ctccatagcc gattccttca
    13081 cctgctggga gttttttgac ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat
    13141 ggataaactt ccggtagtgc gtgcccagag ggattaagtc cgtacattgc tactccataa
    13201 cgaatcatat tgccgattgt ttcatcatgc cataaagccg ttgcactatt gctgacatgt
    13261 acatatcgag gaagttcggg aagttttttc agcacttcta taaagcgttc tttttgcaga
    13321 ttccaatatg tatcatctgc ctgatctgct gtagaaaaat gggtaaataa accttcccat
    13381 tctaatacac gactttcttt gatgaatgca gctgcctctt ttacttcttc aggtgtacaa
    13441 aatccgatac gacccatccc agtgtctgct tttagatgaa tcgaaagagg cgtctctagc
    13501 ttacttttct ctaaaacagc agctgctgct tgcaaccatt cccgtgtacc tgcagttact
    13561 gataaatcat gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt caagataagg
    13621 atgggctgag taaatccagc ttctctcaac tcgactcctt catctaaatt tgaaacacaa
    13681 aagcccgaag ctccacctgc aactgctgcc tcagctgttt caatagctcc atgaccgtat
    13741 ccgttcgctt ttactactgc aaacaattct tttccttgcg gaagtcttgc aacttcattt
    13801 gctacatttt cagttatagc ttttttatgt atcactgctt tcgttgggcg gtgccaagcg
    13861 acaaccatcg tttttccttc tttctagtct tccaaaataa tttgtgcaaa agctgtttca
    13921 tttgtatgag taatactgac ccatacattg ccttcatgag gtgaacgcgt cacaacaggc
    13981 tgaccatttt cattttttaa aatctcgaca tcttgaaagg taacttttcc gatcccagtt
    14041 ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg
    14101 cgatgaaaag gcaaggactg gaatagtttc atttcatcag aggtcaagat tcgtgcaatg
    14161 aattttggtt tttcttcgat cagccgagtg atcctaggaa gttcgactgc atcgatccca
    14221 attcctttta tcatatgctt ttctcttttc aataaattta gaacactctc attctaccaa
    14281 attataacca ttttgccaca aataagtcca caaataacaa aaatgaaagt ttctcttaaa
    14341 aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt
    14401 ttatcattgt gacgtttacc gccatctttt tggctgttat tatttttgct ataaccgcct
    14461 tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt tccgccacca
    14521 cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc tggtgtgatc
    14581 ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa
    14641 tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtattt atctagacca
    14701 ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc tttgaatgct
    14761 tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca
    14821 tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc ttttccggca
    14881 cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat atcatagttg
    14941 tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt cgctacaagg
    15001 atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa
    15061 tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc caattcgtct
    15121 acacggcgtt ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac atcaaacaag
    15181 cgagtcatga tatcgaattt ttcgtattct tttgcgcgga catagtattg atcgatcaag
    15241 tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat gaatttaacg
    15301 ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca
    15361 ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa catttcatct
    15421 gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg
    15481 tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa gccacggatc
    15541 tgacggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt gtcgcgccct
    15601 aaacgatata attcttcttg tgtttggata gctaattcac gtgttggtgc gataactaaa
    15661 ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc aaatgcagct
    15721 gttttccctg tacctgtctg tgcttgtcca ataacgtctt ttccagctaa tgctagtgga
    15781 atcgtttctg cttggatagg cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac
    15841 aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata tggttgttaa
    15901 ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt
    15961 tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc accgaaaaat
    16021 cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct ttatcattat
    16081 tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt tctcttcatc
    16141 tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac agatttttat
    16201 ttcatttgct gaagttgttc taccacttca aataagccca tgctgttgct gccttttagc
    16261 ataatactat ctgttgctgc aagattttgt ttgatagcat caatcaattt attcttttct
    16321 ttttcaaaat agaacacagg tagttggggg tatttttggt tcaattcttc ttttagtgca
    16381 tgcatttgtt ccccataaag gaaaatcatg gcgaatttct caggatcgat atgttcactc
    16441 atttgcgcat gcatatttaa agactctttc cctaattcca gcatatctgc taacacagcg
    16501 attctgcgtc cttttgttgg cagattttta aaagtatcta ataccagtcc catggctgtt
    16561 ggatttgcat tgtagacatc gctcaaaata gctgcaccat ttcctgccgt tagccattcg
    16621 gttctgtttt gagtgataga aacatgggcg agcccttggc gtatctcagg tatggacagt
    16681 ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata acttccaggc
    16741 agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt tccttttgct
    16801 gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt ttgtactaat
    16861 gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa tccgtctgat
    16921 acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga cccaagattt
    16981 tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc cataagtgaa
    17041 agttccgtga tttcattggc gtgatccatg cccatttcca aaacgatctt ttctgtatct
    17101 tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc
    17161 ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt ggtggtttta
    17221 ccattgcttc ctgtaattgc aacgacttct gcgcctactt tttccaagta ataggttgcc
    17281 aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatt ttttggagct
    17341 tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc ttgctggata
    17401 aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc ggttatcttc
    17461 cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct gcccacggct
    17521 tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa
    17581 agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat ttctctattc
    17641 gtatttagaa gctagaatga acgcggttac gcaatcactc cagcctcttc tttatttctc
    17701 tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt cctaactgga
    17761 tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag agtgggtaca
    17821 tactgaactc cgtaaatcct ggcatagagt ttaattcatt caggaataat tcatttttat
    17881 ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc taacatcgtg taagctaact
    17941 tcgcgtactc ttgcgctttt tgataaactt cttctggcac ttcggctgga atctgcattt
    18001 cgattttatt attgatatat tttgcttcat aatcatagaa tgctacgtct tttacgactt
    18061 caccaggcaa agtcgtccga acgtcttcat ttcctaatac agcaacttcg atttcgcgcg
    18121 cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt gctaaagcat
    18181 tttgcagctc ttctcggttt tctgcttttg taatgccgac actagaaccc atattcgccg
    18241 gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact tttttaggat
    18301 tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca ccagcagctt
    18361 gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat acgcctgcgc
    18421 cgacataagg catattcaat gtctctaaga agccttggat cgttccatct tccccgtttg
    18481 gcccatgtaa aactggaaaa acgatggctc cttcttcttt gatttcgccc ggattgatca
    18541 cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat
    18601 ctttgctggc aggtttttct gttagtaatg gacctttgac ccattgtcct tcttttgtaa
    18661 taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact gaaaatgcgg
    18721 aaagaatgga cacttcatgc tctgcgctgc gtccgccata tagtaaagta atcttcaaaa
    18781 gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta gcacaaaaca
    18841 aaaataaacg aataacttat ttataaaaaa acgagccagc ttctaaaaga acgagcgtca
    18901 tttatttttt gtcagctaga ttatttgtcc cgttttttat aaaaagttat gataaagaaa
    18961 aggagggatg atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt
    19021 gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa
    19081 aaaagccttt gaagaactag gccccacctt catcaagata ggacagatgc tttctgttcg
    19141 agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag atagtgtacc
    19201 tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc tttcattatc
    19261 agatatattc gatgattttt caaaatcgcc ttttgcctct gcttctatgg ggcaggctca
    19321 tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag atccaacacc ctaacatcgc
    19381 cgaagagatc cgacttgatc ttcaactatt tgaacgagcg atccctttga tcaaatacat
    19441 tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac gttccttgat
    19501 caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc aaaagaacaa
    19561 tggctggaaa gagatccgca gccccaagat ttatgacgct ttttgttcga aaaaagtgat
    19621 cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta atgaacgccg aaaacaaaac
    19681 tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa caagaagttg caaagctttt
    19741 agttgagaac tttatgaaac aggtgtttga tgatggtttt ttccatgctg atcctcatcc
    19801 agggaatctg ctattccatg tactaacaaa agaagaacag acacaagcta gccgaaaaac
    19861 tgaaacagtc catgaaaaag agttcggttc ctttgctttt cgagcctcca cttctgcaga
    19921 agaccctgtt gcgccatata ccatcaacta catcgatttt ggtatgatgg ggcatctttc
    19981 tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacga aagatgctta
    20041 tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg
    20101 gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta ttgatgaaat
    20161 caacttgcaa gaagtattca cacatgtcgt gacgatttgt catcaaaata atctgcagtt
    20221 cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg tgatccgcgt
    20281 cctcgatcct gaagtttccc tcatggaagt cgcttcgcct tttgctcaac attattttct
    20341 cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat tggaagggat
    20401 gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt ggacttcggg
    20461 acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag
    20521 tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag ggtcctccct
    20581 gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag ggatatttac
    20641 gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga tccaaatgta
    20701 taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca agaaataaga
    20761 aggggtttta cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga
    20821 ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata
    20881 agaacaatca aaaagggaac atggaacaca tttatcctgt ttccctgttc cctttttgat
    20941 gtattcgagc ataaataaaa taccttcgtt acctactatc ttctgtactt ggtaaacctt
    21001 agtcaattgc cttttttaac gcttgacgaa aagacatatg atctttctcc gtaaagggtt
    21061 ttggaccctt tgtccttatg ccagcttttc ttaactttcc actatgataa cgttgttcta
    21121 gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga gtgatggaac actcgcacac
    21181 ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag tcattcttct
    21241 taaccatacc gacgatttta taatccgcac tatcagctcc gcggtctacg tagacgattt
    21301 ggacatgatt agcgtattct ttggtcgtat aatggtccac actggtcacg ataaccacgt
    21361 caagaccata ggattctcct aattttatga catcttcttt tactggagaa ccatctccat
    21421 caatgaatag cctcattctt tttcgccttc ttccataaat gataaacagt tactaatccg
    21481 ctcagtaaaa gaccaacatt gatccatttt gccaagataa gcagttgtac actttctatc
    21541 ggcactgatt catggttcag cgtgatcaat aatagatgga ttggctctcc catttctttt
    21601 gctaaaacaa acaaaagcgt catcaataaa aactggtgga tcacactccc tttattttga
    21661 gtagtatact tgacaacgat ccggcgatcc attggaatag ctgtatcagt ttgataatat
    21721 tccttattta gccagctgcc tgttactacg tgccagaaac taagcagaaa gacacctatc
    21781 gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac tcagtccaag caattgcata
    21841 gccaatggag accatgttaa acgtttcaaa atccatggtc cgactatttt tgctgcaatc
    21901 aagcctgctg cataaggcaa atacataaaa atcgaaagtt tttctaatcc gtacagtatg
    21961 ccaacataaa aactgctaaa taaaaaaagg aaattcccta gcattccatt tagaaaagta
    22021 aacagattta gccagcttgg caacttccat tccttcttct ggtgtcctaa aacaaacacg
    22081 aacaagatga acagcagcaa gaaaccataa atagcaagat caaaaagcct ttcatcgaat
    22141 aacaatcggg catttctcaa aagaaacaat aagccaaaga atatcaaaaa taaaacaagc
    22201 tctcgcgtag ctaccagatt tcttttgatg tctttgaaat ccagttcgta acgcggataa
    22261 tgactaaccg tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca
    22321 atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca gatagctgca
    22381 aataagtact ttttcccatc catattgata aaaccttgcg tcttttcgtg aaaattgacc
    22441 gtggtattag caggtacaag ccatgctgca ctcagcccta gcaaaacact tgaaaaataa
    22501 aataaaggaa aataaaatac gcctaatatt ccaagcaatg aaccagcacc accaatcagc
    22561 agagcgatca tcagcaacgt gtaactatct agaccaaatc gtagactgtt gactaaaaat
    22621 agtccagtca tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag ccaattatgc
    22681 gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt acttaagata
    22741 aagggcgttg tttgtgtaaa attacgccca aatcgtttga gtttttcgaa catgacaatg
    22801 gctccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa ggggaaataa
    22861 accaatttta cagaatatat tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt
    22921 ctgcattttt acggtacttg attactcaag cagacaagcc cttctccaca aaagtcttgc
    22981 ccgacacccc tgcatccggc aggtgataaa gatagaaatc aattatcctg tgcctccaaa
    23041 atcaattagg agctctgtaa accagagtcc ttctcgaccc gtcgcataaa tttactttaa
    23101 cacagttcct atgtttatgc gagagctttt ttcattttat aatcgaccat ttgaaatcct
    23161 tgaccgatat ctgaaataag ttcttctgta atctcaaatc cttcatgctt ataaacctcg
    23221 attgcttgat gattcccctg attcactcgc aaaaagatct ctctttcctt tgtttgcgca
    23281 gcaagttctt cataccagcg aaaaactgaa gaagtcaatc cttgaccacg tactttattt
    23341 agcaaatata atttacttaa ataaattttt ccatttatct cttcataagc agtgtaaccg
    23401 acagtctttt cttcgctttt taataaaaag taggacacac cttctgtcag ttctttttga
    23461 atctgttttt ttgactgata agttttcagc atgtaatcca cttgtttttg accaatgatc
    23521 ggcacaaaag cttccggcca tacttcttgt atgactgcca tcagctcatc cagttcattt
    23581 tctgttactt ttattttttc catacaccca cgtccttttt tacattataa atgaccttgc
    23641 gcttgatgga ataaaaagag catgatcatg ctatacacac ccaccgacca tggtttcaag
    23701 aaaatcacaa tctgcataaa ttccctaaaa gataatttcg tcaaaccagc tactagacag
    23761 atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt tttaatatct
    23821 ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact tttttggcga taaaataagc
    23881 tggacaaagg ctacgccata gtaacggaca agcaaaaatc cgattgcttc cccaatcact
    23941 aggccaatac aactatataa aagcccaaat ccattgccaa acatcagcat gcccgctact
    24001 gaggagatcc ctcccggcaa aataggaatg atcacttgga tcacttgcaa cagaacaaat
    24061 atcatcactg catacttgcc aaattgctgg ataaacgctt gcaaagacgt gctcgatcga
    24121 aaaatcccat gatgataagc ataaagaatc aacacaaaga aaaaaaacag accaataatg
    24181 ggcatgatct gaagacattt ttgtaaaact gatccttttt tagacatcta tattccccac
    24241 atcttttttc atttattaaa actgcctcct tatgatgtgc accccaaaag ttagactaga
    24301 aatctaatta aaaggggtgc tttttttgcg gaaatataca tttgttttta agaaaaaagt
    24361 agtttcagac tatttaaata acgaaggcgg ctacaaatat cttgcacata aatatcaaat
    24421 aaatcgtaca ctggttagac attgggtaag gatttataac tatcatggtt gggaaggctt
    24481 ggttggaggt ggcaaaagct acactacaaa atttaaactt gatgttatag aatatatgga
    24541 aacaaatggt ctttctatcc aagaaactgc taaaaaattt aatatcggtt caaatagaac
    24601 tctaagtaaa tggatagagc aatatgaaga aggcggtgct tcttcacttg agagccaaaa
    24661 aaggggcaga aaaattagta tgaattccaa gctaaacatt cctaaaaaac ttaaagatga
    24721 gtctcttgaa gaagaagtta ttcgtttaag agcag
//
LOCUS       DAZKCY010000030        23885 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000030 DAZKCY010000000
VERSION     DAZKCY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.030"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACG8XC_001124"
     CDS             complement(55..789)
                     /locus_tag="ACG8XC_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP9190836.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACG8XC_001125"
     CDS             1116..1526
                     /locus_tag="ACG8XC_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP9190837.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACG8XC_001126"
     CDS             1519..2220
                     /locus_tag="ACG8XC_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFP9190838.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACG8XC_001127"
     CDS             2220..3806
                     /locus_tag="ACG8XC_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9190839.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACG8XC_001128"
     CDS             3825..4046
                     /locus_tag="ACG8XC_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFP9190840.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACG8XC_001129"
     CDS             4043..4804
                     /locus_tag="ACG8XC_001129"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFP9190841.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACG8XC_001130"
     CDS             5184..5693
                     /locus_tag="ACG8XC_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP9190842.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACG8XC_001131"
     CDS             complement(5768..6307)
                     /locus_tag="ACG8XC_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFP9190843.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG8XC_001132"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG8XC_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP9190844.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACG8XC_001133"
     CDS             7320..8171
                     /locus_tag="ACG8XC_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP9190845.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACG8XC_001134"
     CDS             8199..8834
                     /locus_tag="ACG8XC_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFP9190846.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACG8XC_001135"
     CDS             8854..9627
                     /locus_tag="ACG8XC_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9190847.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACG8XC_001136"
     CDS             10035..10832
                     /locus_tag="ACG8XC_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFP9190848.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACG8XC_001137"
     CDS             10810..11223
                     /locus_tag="ACG8XC_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9190849.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACG8XC_001138"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACG8XC_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9190850.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACG8XC_001139"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACG8XC_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP9190851.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACG8XC_001140"
     CDS             16000..16560
                     /locus_tag="ACG8XC_001140"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFP9190852.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG8XC_001141"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG8XC_001141"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFP9190853.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACG8XC_001142"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACG8XC_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFP9190854.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEELTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACG8XC_001143"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACG8XC_001143"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP9190855.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8XC_001144"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8XC_001144"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP9190856.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8XC_001145"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8XC_001145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP9190857.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACG8XC_001146"
     CDS             21907..22485
                     /locus_tag="ACG8XC_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9190858.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACG8XC_001147"
     CDS             22537..23019
                     /locus_tag="ACG8XC_001147"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFP9190859.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACG8XC_001148"
     CDS             23136..23510
                     /locus_tag="ACG8XC_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190860.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7809 a         4291 c         5123 g         6662 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaatcg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga actaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg
    23881 aagtc
//
LOCUS       DAZKCY010000031        23674 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000031 DAZKCY010000000
VERSION     DAZKCY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.031"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(609..2105)
                     /gene="lysS"
                     /locus_tag="ACG8XC_001149"
     CDS             complement(609..2105)
                     /gene="lysS"
                     /locus_tag="ACG8XC_001149"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9190861.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2216..3220)
                     /gene="dusB"
                     /locus_tag="ACG8XC_001150"
     CDS             complement(2216..3220)
                     /gene="dusB"
                     /locus_tag="ACG8XC_001150"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP9190862.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3221..4108)
                     /gene="hslO"
                     /locus_tag="ACG8XC_001151"
     CDS             complement(3221..4108)
                     /gene="hslO"
                     /locus_tag="ACG8XC_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP9190863.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4325..6436)
                     /gene="ftsH"
                     /locus_tag="ACG8XC_001152"
     CDS             complement(4325..6436)
                     /gene="ftsH"
                     /locus_tag="ACG8XC_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP9190864.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     TNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6526..7071)
                     /gene="hpt"
                     /locus_tag="ACG8XC_001153"
     CDS             complement(6526..7071)
                     /gene="hpt"
                     /locus_tag="ACG8XC_001153"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP9190865.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(7071..8516)
                     /gene="tilS"
                     /locus_tag="ACG8XC_001154"
     CDS             complement(7071..8516)
                     /gene="tilS"
                     /locus_tag="ACG8XC_001154"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP9190866.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8636..9106)
                     /locus_tag="ACG8XC_001155"
     CDS             complement(8636..9106)
                     /locus_tag="ACG8XC_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFP9190867.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9152..9577)
                     /locus_tag="ACG8XC_001156"
     CDS             complement(9152..9577)
                     /locus_tag="ACG8XC_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFP9190868.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9644..9913)
                     /locus_tag="ACG8XC_001157"
     CDS             complement(9644..9913)
                     /locus_tag="ACG8XC_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFP9190869.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9924..11519)
                     /locus_tag="ACG8XC_001158"
     CDS             complement(9924..11519)
                     /locus_tag="ACG8XC_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9190870.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGVALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11534..15055)
                     /gene="mfd"
                     /locus_tag="ACG8XC_001159"
     CDS             complement(11534..15055)
                     /gene="mfd"
                     /locus_tag="ACG8XC_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP9190871.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(15072..15632)
                     /gene="pth"
                     /locus_tag="ACG8XC_001160"
     CDS             complement(15072..15632)
                     /gene="pth"
                     /locus_tag="ACG8XC_001160"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP9190872.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15985..16959
                     /locus_tag="ACG8XC_001161"
     CDS             15985..16959
                     /locus_tag="ACG8XC_001161"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP9190873.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17129..18529
                     /locus_tag="ACG8XC_001162"
     CDS             17129..18529
                     /locus_tag="ACG8XC_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFP9190874.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18607..19329)
                     /locus_tag="ACG8XC_001163"
     CDS             complement(18607..19329)
                     /locus_tag="ACG8XC_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9190875.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19408..19914)
                     /locus_tag="ACG8XC_001164"
     CDS             complement(19408..19914)
                     /locus_tag="ACG8XC_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9190876.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            20037..21836
                     /locus_tag="ACG8XC_001165"
     CDS             20037..21836
                     /locus_tag="ACG8XC_001165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFP9190877.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21904..23478)
                     /locus_tag="ACG8XC_001166"
     CDS             complement(21904..23478)
                     /locus_tag="ACG8XC_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP9190878.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6467 a         5110 c         4304 g         7793 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa gcgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcggca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt ttaacgactc
      301 agttatctta tcatgtcgtt agtaacatgt caataacttt tttgttatgt ttttgaataa
      361 atattttctt ctgtcttttg gacaagtaat aatataccaa ctaaatttag cttggtcaac
      421 gaattttttg tattttttca agtttttttc aataaacgat tattcataac ttttctttct
      481 ttatcgttcg ttttcgtctt ctttaatgtc tagaatagtc ggcattagct tttgaacttt
      541 cgttattatt tattcctttc tacacagcaa aagctgccta aggaattcct tagacagctt
      601 ttattcgatt atctcattgt tgggaatagc aaaacatccc gaattgattg tgcatcagtt
      661 agcaacatta ctaatctgtc gatcccgatg cctagaccac cagttggcgg cataccgtat
      721 tctaaggctt ccaagaagtc ttcgtctaca ccgtgagctt cgtcatttcc tagttcacgt
      781 tctttttctt gcgcttcgaa tctttctctt tgatcaattg gatcgttcag ttctgtaaag
      841 gcattagcaa attcgcgccc cactatgaat agttcgaatc gatctgtgaa tcgaggatct
      901 tctggatttt tcttcgctaa aggcgaaact tcgactggat gtccgtatac aaacgtcggt
      961 tgtgtcaatg tttcttcaac aaatgtttca aagaattcat tgataacatg tccgacaccc
     1021 atagcttcgg ttacttctac attgtgtttt tcggctaaag ctaatgcttc ttccaatgtc
     1081 atttcttgcc agaagtctac accagtttgt tctttgattg catctaccat atggatgcgt
     1141 ttaaagtcac tttctaagtc gacagcttgt ccatcatatg tgatttttgc tgttcctagt
     1201 actttttcag ctgcgttacg gataatacct tctgtcagat tcattacatc attgaaatca
     1261 gtatatgctg tgtatgcttc taacatcgta aattctggat tatgtgtagt atcaattcct
     1321 tcattacgga aaacgcggcc gatttcataa actttttcca ttccgccaac aattagtcgt
     1381 tttaaatgaa gttctagcgc aatacgcaaa tataaatcaa tgtccagtgc attatgatgc
     1441 gtgataaatg ggcgtgcggc agctccacct gcctcattgt gcaatactgg tgtttctact
     1501 tcaatatagc cattgccatc taaatagcga cggatttcgc tgataatttg gcttcgtttc
     1561 ataaatcggt caaagctttc gcgattactg attaaatcca agtagcgttg tctgtagcgt
     1621 tgttcaatat tcgttaagcc atggtattta tctggcagtg gacgaagtgc ttttgacaaa
     1681 atcgtgattt ctgaagcttt gatagaaact tcgcctgtgt ctgttttcat gatttgtcca
     1741 gttacaccaa agaaatctcc aaggtctgca tgtttgaata cttcataggc ctcatcacct
     1801 acttggtctt ttcttacata aatttgaatt tggccttcac gatcttgtag atgcgcaaat
     1861 ccagctttcc cttttcctcg gcgagtcatc atacgaccag ctacactagc ggtcaatccc
     1921 atttctgcta gttcttcttt tgttcttgga tcaaatagtt catgcaattc ttctgagttg
     1981 tgcgtgcgtt caaatcgttt tccaaatgga tcgattccat cttcacgcaa ttgttccatt
     2041 ttttgacgac gtacaagcat ttgatcattt aaatcttcta cttgatgttg ttgttcctgt
     2101 gtcacaaaag ttcctccatt ctcatgttaa tctctttcct ataatgccaa tgaaacacaa
     2161 aaaaagcaag ccgaacttgg aatttcggct tgaaaattca cgaaaaactt agaaatcaca
     2221 acgtttgttt caatagtact ttttccgctt tttctacaaa ttgatccagc aggtcattga
     2281 tttcttgctg tttttctgtc tgattgattg ctactttcac tttcgctgct cgaggaatcc
     2341 cttttaaata ataagccgca tgtgttctga attctctaca agctactttt tctcctttga
     2401 gatctgctaa gcgttgtaaa tgaagtttag ctgtagcaat tttttcggct ggtgttggtt
     2461 caggaatcag ttctcctgtt tcgagataat gctgtgtgcg gtgaatcatc catggattgc
     2521 ccagcgctgc acgaccaatc atcacaccgt ctgcaccgac atagtcaagc atccattttg
     2581 catcttctgg cgttttgaca tccccgttcc ccataaatgg gatcgtcaaa tgttttttca
     2641 cttctttcaa tacttcccag tccgcttttc cttcatacat ctgaacccgt gtacgtccat
     2701 gcatagcgat ggcacttgct ccagctgatt gtgctgctaa tgcattttct acagcaaaga
     2761 cgtgctcttc atcccaacca atacgcatct tgattgttac tgggatattt actgctgaag
     2821 aaacagcttg gaccatttcg tatactttct caggatctaa aagccatttt gctcctgctt
     2881 ctgctttgat tactttattt actggacagc ccatattgat atctattata tcggcagctg
     2941 tgttttcttc gacaaattga gcagcttcaa ctagactatc tttatttccg ccaaatatct
     3001 gtaaacttaa tggatgttct gtttcttcaa tgtggagcat ctctaaagtt tttttgtttc
     3061 tgaaatgaat accttggtca ctgatcattt cacatacgac taagcccgca ccaaattctt
     3121 tgacagtgac tcgaaatgct gaatttgtta ttccagccat cggtgcaaca acgacacgat
     3181 tagggatttc cacattgcca attttccatg tgtgttccac ttaaatccct cctaaggctt
     3241 ctttcttcaa tccatctaat tcttcttcgc tatacttgta tttgttatta cagaaagaac
     3301 aaaccgcttc tgcaccgtga tcttcgtcta tcattgcttg gatttcttct gcacctaacg
     3361 cgataattgc ttctgcaaat ttttcttttg aacagtcaca tttgaattga acaggcattt
     3421 tttccaaaat gttcaattct tctgtaccaa gcaatccttt caatatttct tctggtgtct
     3481 gtccttcatc taagagggta gagattcttg gtgtcgtttt taattgttct tcgatcttgc
     3541 tgattgtttc ttcatcggca cctggcatga tttggatcat gaatcctccc gccgtcttga
     3601 tgctgtcgtc agtattgacc aatacactca gtccaacagc tgaagggatt tgttctgaga
     3661 tagcaagata gtaagtaaaa tcttcagcga tttctccaga aacaatcggc gtttgcccag
     3721 aaaaaggttc ttttaatcct aagtcttttg tgacagtaaa cattccttgt gttccaactg
     3781 ctccccgcac atcgatttta ccttcactat ttagaggaag actgagatgc ggatttttga
     3841 tgtaaccttt cacatctccc tttccatttg catctactac aattcctgtt gctggaccat
     3901 cgccttggat tcgaacagtt agtttgtctt ctcctttgag cgtcgcacca agtagcagac
     3961 taccgaccaa tgtccgtccc aaagcagctg aggaggtgtg ccaagtatca tgacgtcttt
     4021 gtgcctccgc tacggtattt gttgcttgaa cggcgtatgc tcggacgaat ccgtcgtgag
     4081 caagtgcctt aaccaaataa tctttcattt gttatgtact cctttttccg tttttaaact
     4141 catagtgaat catactagca actactgctt ttttcaactt tggaatgctt cttttaaaaa
     4201 taattcagaa tcatctagaa aacaggacat caccctattt acaacttaaa taaagaaatc
     4261 tcccgaaaac cggtttttgt tccggttttc gggagtcata tcagatcaat tgacctatat
     4321 ttttttattt gtattgatcg tctgaatgat tatcttctga ttgttgagaa tctgaatcat
     4381 gttctgcttt attttcctca ttcagttcat gttttgcttc ttcaaaatca cgtttttctt
     4441 ccgcttgttt ttcagcgtct ttttcttcca aagcacgttt tgcttcttca aaagttttcg
     4501 cttctttttc gcttggataa tcatttgact cagtaccttc aggcatcaca ccgtgttcaa
     4561 acaatgactt gattgctttg gcatccaatg tttcgaattc caaaagtttt tcagcaatca
     4621 gtttatgctg tgcacgatga gcttcgatga tttctcgagc tttatcatga gcttccatca
     4681 agatttttcg tacttcttga tcaatctcga aagcgacttg ttccgagtaa gcttttgttt
     4741 gaccatagtc gcggccaaca aacacttgat ggtttccttc atattgaaca ggacctaaac
     4801 gatcactcat tccatattct gttaccatgc ttcgtgctag agctgtcgct tgctcaaagt
     4861 cattggaagc accagttgat tggacattga agatgatttc ttcagctgta cgtccaccaa
     4921 gtaagcctac gatttgttca aacatatctt ctttcgtcat caagaactga tcttcttttg
     4981 gtaaagcgat catatatccg ccggcgcgtc cgcgagggat gatcgttact ttgtggacga
     5041 cccgagcgcg actcaatact aatccgacaa tcgtatgacc agcttcgtga taagcaacca
     5101 tttcgcgttc ttttttgctg attacgcgat cttttttagc tggacctgca atcacgcgat
     5161 cttccgcttc atcgatatca gaagcatcga ttttcttttt atttcttcta gcagctacta
     5221 acgctgcttc attcaagaca ttttctaaat ctgctccggc aaaacctggt gtctgttgag
     5281 ccacaacttt caagtcaacg tcatctgcca tcggtttatt ccgtgcatgg actcgcaaga
     5341 ttgcttcacg accttttaca tctggacgtc caactaaaat ctggcggtca aaacggcctg
     5401 gacgtagtaa cgctgggtct agtacatcag aacggttggt tgcagcaatg acgattactc
     5461 cttcatttcc gtcgaagcca tccatttcta caagcaattg gttcaatgtt tgttcgcgtt
     5521 catcatgtcc gccgcccata ccggcaccac gttgacgacc taccgcatca atttcatcaa
     5581 tgaaaatgat agctggtgcg tttttcttcg ctgtttcaaa caaatcacga acacgacttg
     5641 ctccaacacc gacaaacatt tctacgaaat cagaacccga gatcgagtaa aacggtactc
     5701 cagcttctcc ggcaacagct tttgctaata aggttttacc tgttcctgga ggtccttcta
     5761 aaagtacacc tgccgggata cgggcaccta attcaacaaa tcggcgagga tctttcagaa
     5821 attcaacaac ttcaactagt tcttgttttt cttcttccgc acccgctaca tcagaaaaac
     5881 gtacgcgatt agcttttttg tctgcttctt tggcttttga tttaccaaag ttcatgacac
     5941 ggccgccacc gccaccgccg ccgccttgtt gtcccatcat catgtaaaag aaaaagatga
     6001 tgattacgat tggcaagaag ctaagcaata gggaaaccca tataccgctg cttgattctt
     6061 cttttactgt tgtagcaact ttgttttctt ttgccagttc tgttacttgg ctcaatgttg
     6121 tatcatttgt caaaatgatt gttgtgaaat gggtagtagt cgtatctgtt gaacctaaga
     6181 tagctaaccc accgctgtta gcgacggtct gctgttcttt gtattcacca gtgatcttat
     6241 atacaccatt tgcaggctgc acttcaaatt ctttgatctt gcctgcttgt agttgttctg
     6301 tgaattttga gtactcgata tctgacgatc gctggccgtt attaccaaaa atgaaataaa
     6361 cgaccattac catcgctaag attacgagga cataatacaa ggcatttttc atcatgccgt
     6421 tgttcttttt gttcatagtt gccctcccta agcgtcaatt taatttaaca gttttttatc
     6481 aatcctgacc attctattgt accataattt aattgagata cagcattaat ttgattgata
     6541 aacactaggt ttcaatacac cgatgtacgg aaggttacga tatgcttctg cataatccaa
     6601 gccataaccg accacaaatt catttggtac gttgaagcca acataatctg cttcgatatt
     6661 tactactctt ccttcaggtt tgtcaagtag tgtaacaatt ttgactgatt ttgctttgcg
     6721 gtatttgaaa agatccacta aataagcaag tgttcttccg ctgtcaatga tatcttcaac
     6781 gatcaacaga tcgcgacctt ctacattcgt atctaagtct ttgacgattt ttacttcccc
     6841 tgacgagact gttgcattcc cgtagcttga aacatccatg aaatccaatt ccaaatacgt
     6901 atcgatagaa cgaacgatat ctgccatgaa tggtaccgct ccttttaaga cacctactac
     6961 taggggattg gtgttttggt aaatttctgt taactctttg cctagttctt tgcaacgaac
     7021 ttgtatctct tcttgtgaga tcaaaattct ctcaatatcc ttttccaaca tcaggttctc
     7081 cttccaattg cttctttttg atatttgtaa agaagtatgt agtgtatttt atcagtttct
     7141 tcacgaatac tcaaatatga aaaggcaaaa ggtatcaagc tatatatatt tcttttggca
     7201 tctgtaatga tccaagcatg ctgcctttgt gaattaggta ctttctgatc gataaaatat
     7261 ctagatactt tttttgtgag attctctgtt aatttaatgc ggtctcctgc ttttcttttt
     7321 tcgacataca gttgcacatc agcaggaatc caaatgtcat gagaaaatag agaccatgaa
     7381 tctgttgctt tgatgtcccc tgcattttca ggtttgacca gaccaatcca ttctgtttca
     7441 ttcaaatata ttccttgttc tggaatcagc aacaagcgct ggtcttctgt ctggctagtt
     7501 gcttgttttt cttttttaga gagtacaaca aaatgatact ctcttttcag tatccaatta
     7561 ttgccaatac tgagttgcca ttggcttttt ttctgttgcc actggtctaa aatcgactcc
     7621 atttgttgtt ctttcaaaat aattcctgtt tttgagaatg ctttgtcgaa aaaggagtaa
     7681 aaaaaatagt atcgttcagc atctgtcatt tcttcaatca tttctgcaga aaatcgaaat
     7741 tgttcctccc actgttcgac ttccttttcg atcaactgtc ccatatattt ttgaatgact
     7801 tggtctgccc attggatctg ttgcgaaaat tgctggaaat gtctcaacgc ctgtgtattt
     7861 tcttgtttca attgaggaac taccaaatga cgaagccgat ttctctggat atctaacaat
     7921 tggtttgtct gatcctcaaa gtaaacaagc tgcgattccg ctgcataagt atataggtct
     7981 tctttgctaa aactcagcaa cggacgaatc agtctgcccg tagcaaatgg ttgtacttct
     8041 ttgattccag aaaaagtatt taactgtccc ccacgaacga ttttcattaa caccgtctcg
     8101 atttgatcat ctccgtgatg agcagtcatc aacgtgtcat attgtccttc ttccatcacg
     8161 cgagtgaatt cttgataacg aaagttacga gcttctgttt ctatggcgga tttggctgga
     8221 tcttcccaaa tacttacata taaagggaga ttcttctctt cacaatatga gcgaagatag
     8281 gcagcttctt ctgctgattc ttctcttaat tggtgattga tgtggacgat acctaaagaa
     8341 aaaccgattt ggcgttgcac ttgtaaaagt aaatggagca aaaccatcga atcaacacca
     8401 cctgataccg ctgctaaaat tctcgatcct tgttgccaga atccttgttc attcccgtgc
     8461 cgaataaatg actgctgtaa tagcggtgct tttttcccgc ctttttcaac gttcatcctt
     8521 tccacaccct ttcttcctaa aaaaaagaga gcgggaaaac gtctaaaacg acaattaacc
     8581 tgccctctgt tgatactatg acccaaatag tcgcactatc attatttgcg gctttttagc
     8641 tgcgacgtcc gccacggcca ccgcgctttc cttctgtgtt tcgcttcaat gaagataagc
     8701 ggtcatcgct atctttcaaa aatgaactca tcaatgaatc gaagtcttct ttttgagctg
     8761 aaggttgact gcgagaaaaa gattttttct gtgctggacg atttcggttg tcacggggat
     8821 ctttttgttg atatttttta ggacggcttt cttctccaga ttcagcaggt tgttcctgcg
     8881 ctttccggat agatagtccg atttttccat catcatttac tgaaataact ttaactgtta
     8941 cttcatctcc tacacttaaa acatcatgga tgtcttttac atagccattt gacacttcgc
     9001 taatatgaac tagacccgtt tttccagcac ctagatcaat aaacgcacca aaatttgtga
     9061 ttcctgatac ttttccttgc agttttgctc ctacctcgat tgacataaaa aaaatgttcc
     9121 tcctaattat ttcccttttt tatattcctg cttattttgc agttgtagaa ctagttgatt
     9181 gatctgtttc atttgtcgag ctgtctgtat ttgattctaa tataggataa actttttcac
     9241 cgtcttttga gtaaaagaat ttgcttctgg caagtttagc tacatactca tcatctttca
     9301 gcaatgaaac atcttggtgc agctgatcaa cgtctgcgtt gacttctttc atttcagtat
     9361 ttgcttcgac tctcaattcg ttcaattgat gcatcctttg catgtcatgg aaaatctgca
     9421 cacctattat cgcgaaaatg accaaagcac caacaaaaat tgcagccagt ctgcgtcttc
     9481 gaaaaatcag ttgacgttgt tgttgctgaa attcagcata ctttttcttt gcatattccg
     9541 tatctagcgc agcgatttta ttgctttttt tcgtcacttg gattcgctcc aatctttttg
     9601 attgtctttt tcattatacc aatatcgtga aagcttgtct aatctatttc tctagtgttt
     9661 ctgtgcgatt ttcagaaata atttcgtaca ttttttgtgc atcttctttt ttggttgatt
     9721 catgtaattc cgtaattttc acttccaacg ttttgttgcc aaactggact ttcaaaatat
     9781 cgcctacttt gacatcactg gaagatttcg ccaatttacc gttgatttga attcttcctt
     9841 tatcagctac ttcttttgct actgatcgtc ttttgatgat tcgggaaatt tttaaaaatt
     9901 tatctaatcg catatcttat tcctcacttt ttaatttttt tatttaatcg caggattttc
     9961 ttaccaaaag gcaacattag ccattctcgt actgtaaaca gacgaagacg aatcactgca
    10021 taaatgaaca cagaagcccc gatcccaact cctatcacgc tgacaaaaaa agcagtggaa
    10081 cgatgagtta caggaccaaa tactatattc aatacaccat aatacagcag cagtactact
    10141 gtcatacaaa gaaggctgaa catcaacttc cgtccaaaat gccgttcttt ccaaaaagca
    10201 tttagctctt ttgtttcaat tcgaacaaaa taccaaagcg tcacacctaa tcctaaaagg
    10261 gtagacaggc ttgcacctgc tgttcctaat aaaccggtca gaaaagaagt agtcaatcct
    10321 tttaccaata gtccccatcc tgcgcctttt aaagaagggc gaaacgagtt cttgctttga
    10381 gcaatacttt gataagcttg gatgactgca gtgaaagcaa tggaaaaaac aaataataca
    10441 agcgctgcat ttcccgcata atctttaaac aacgcataat tgatataagg gagcagcaag
    10501 gctaatccca aagacgcagc taatgctaaa gcggtcgtca atctaagata gattttggct
    10561 gtctgtaaaa actgacgata gatccggttg gtcaaatgac gagtcaacgc aggcaggaat
    10621 gtcgcactta atgcagtggc aatcactaaa cctaattgta ctaaaggctg acctcgatca
    10681 tagacccctt ttgctatctt cgcaccgtat tctgacagtc cgtaaacttc caaagcattt
    10741 tttacaaaga aagagtcgat cagctggaaa aagataagca atccactata aatcgaaacc
    10801 agtccgcctt ctaccagaaa acgccggatc aagggctttg acggctgttt gaacaatgga
    10861 aaccgtcgga agcttaacgc tactccatga atcttttgtt catagtagtg aaggatccca
    10921 tatgcgcata aaccgccaaa aacagctcca ctcattgctg ccgtccctgt ttggtacacc
    10981 gtccatccaa gctttctaaa agcaaacgca ctacagacaa tcacaaaaac acgaacgaac
    11041 tgctcaacta cttgagaaac agcagttggt tccatcaaga atcttccttg gaaattccct
    11101 cgataaaaag atagaccagg catcagcaaa aaggtaaagg aaactacttg gatcagcggt
    11161 ttcaattgta tgtctcccat catccaagca atccagctgc taaatgcaaa gacaaacaac
    11221 cacaaaaaga cacctgtcca aaatactaag ggataaagat tttgcaaagc ttgctttcgc
    11281 tcgaaaggat tctttctttc tgcaacgtac tttgatatga actgcggaag accggataaa
    11341 gctaaagtca ttgctatccc ataaattgga taaacttgct gataaacata aaaaccttcg
    11401 tcgccgacta agttttggag gggaattcgg taaagggcac ttaatacttt tgctatgaaa
    11461 gaagcaatcg tcaatacaaa tgctccttgc atgacgcgcc gcatttcttt atgatccaaa
    11521 tcattgctcc ttcctacgct gacagatatt tttgctcacg caacgctttg acaaactttt
    11581 ggatttcctg aagccatact gcttcttcca tttggttggg gatcgtcaag cgtatcacca
    11641 tctgttcttt ttcaacgcct agactggctt ttagttttgt cgctgtcagt gcttcgaata
    11701 actgttccac actatagcgt tttgttccaa ctttacttag agtaaagact atatgcgcct
    11761 cctgtttcct tatcgtttct aggagcgctc gatcgccatc cattttgatc tgcccaattg
    11821 tcaagagatg tgctacttct tctggatatt ctccaaaacg gtcgagcaga tcatcttcta
    11881 gttcatctag catttcttgc gaatcaagtt cacgaattcg tttatagatc tcgattttct
    11941 gccgttgatc tgccacgtat gtttcaggta gatacgcatc gacaccgagg tcgatctcaa
    12001 cagtggtctt ctccacttga ctattctttc cttgtttacg agaaacagct tcgcttagca
    12061 tttgagaata catatcgaaa ccgactgcat caataaatcc atgctgctga gcacctaaga
    12121 gattacctgc accgcgaatg gaaagatccc gcatcgcaat cttgaatcca gatcccagtt
    12181 ctgtaaaatc tttgattgcc tgaagacgtt tttcactgac ttcgttaagg atcttctgct
    12241 gctcatacat aaagtaagca tatgcgacac ggctgcttct tcctacccgc cctcgcaatt
    12301 ggtataacgt ggataatccc atataatcag cgttctcaac gaacaacgta tttgcgttcg
    12361 ggatatctac tcctgtttca atgatggttg tagtgacaag aacatcatat tgaccttcaa
    12421 taaattcaaa aagcgtattt tctaattgcg cctctgtcat ttgcccatga gcataaccga
    12481 tccgtgcttc cggaacgagt tcttggattt cttctacttt ttgttcgatc gtttctaccc
    12541 gattatataa atagaatact tgtccacctc gtcccatttc tcgatggatt gcttcgcgaa
    12601 tagccccagg atttttttcc ataacatacg tttggatcgg ataacgattc gcaggcggtg
    12661 tttcgattac tgaaagatcc cgcacgccta acatcgacat atgcaaagtt ctcggaatcg
    12721 gtgttgcagt cagagtcaaa acatccactt gtgcacgtaa ctgtttcaat cgttccttat
    12781 gttttacacc aaaacgttgt tcttcgtcaa tgatcagtag acccaaatcg ctaaattcaa
    12841 tatcttttga caaaattcgg tgggttccta caacaatatc aacttgacct gtacgcagtt
    12901 gttcgatggt ttctttttgt tgctttcttg ttctgaaacg acttaatagc ccgatattta
    12961 cggggaaacc ttcaaatctt tccagcatcg tttcatagtg ctgctgtgcc aagatcgttg
    13021 ttggaactaa aaaggctact tgtttgcttt cctttatcgc tttaaaggcc gctcgaagcg
    13081 ctacttctgt tttcccataa ccaacatcac ctacgagtag tcgatccatt ggtttttctt
    13141 tttccatatc ccgtttgatc tccgctgcac ttctcaactg gtcatcagtt tcagagtagg
    13201 ggaaggcatt ttcgaattct ttttggtagg cgtcatcagg tccaaaagca tatccttttt
    13261 ctgactctct tgcggcatat aatttgatta gatcgtcagc gatatcctcg atttttgaag
    13321 agacttttcg cttggttttc gtccattcac ttccgcctag cttattgatg cgaggcgctt
    13381 tggcttcaga ggcgacatat ttttggatca gattcagttg agtaacagga ataaacagtt
    13441 tgtcgtcgtt ttgatacaaa atagtcatat agtcttggtg aacgccatct acttctagcg
    13501 tttccatccc aatgtatttc ccgataccat gattggcatg gacaacgtaa tctcctgctt
    13561 ttaactcatt atagcttttc aatcgctctg cgtttgttac tgtttgacgt ctcgtttgtt
    13621 ttttcgtcgt tttttggaaa atttcttttt ctgtgatagc tactattttt tcttgaggaa
    13681 gttcaaaacc agcctgcaaa gctccttcaa ctaattgtac ctgtcctctg atcaatgcat
    13741 ttttttctgt ttctactact aatatgtctt catctcgaag catttgttct gctttgcgta
    13801 ttctgtcctt tgtcggaata aagatcacaa cagtctgttt ttgcttgcgc caacggtcca
    13861 tttctgtttt cagcaatggc atttgtccga agaactgttg catcgtccga tattgaaaat
    13921 tatggattgc ttggaaacga agatttccca tccctttttg gaagagtgag aaaaaggttg
    13981 tggtgaactt ttccttctgc acgatttttt gtatatctgc tgaaaatgtc tgctcagaaa
    14041 acacccgcaa ttcgctgatt ttttgagtct gccattctgc cgcttccctt tcgatttccc
    14101 gatttgtttc catgattcgc tgataatcat caacgactag taaactattt gaagggaaat
    14161 aatccaagat cgtcacagat tcttggtaaa gaagatctga gtagaatttt gcttcttctg
    14221 tcgggacacc tgcttgccag cttgaagtca gctgaccgaa gtaatcttgc aaaaactcgc
    14281 ggtctgcttc ttctgtggtc gcagcaagcc gtttttctag cattttttgc atttgctcta
    14341 ttccatgttc tagatcctct ggtccgtaga cttgttcact cgttggggaa acccagattt
    14401 cttccgtttt ttcgattgag cgttgtgttt ctgcttcaaa ataccgcata gaatctatct
    14461 ctacatcaaa tagttctaca cgaacaggat aggttgtcgt taacgggtaa atatcgataa
    14521 tgcttcctcg aatactgaat tctcccggtt ttgcgaccat ctcttctcgt acatagccca
    14581 tcaaagtcaa ttggcgagga agattttcca cgtctatctc atcaccaata tgccaattca
    14641 attgcgcctg tagccaagtt ttcttattcg gcagtcgttt gtgcaaagat gcgactggca
    14701 acaaatagat acctgctttt ttttcttgaa gtgcgttaag cgttgcaact ctctcgcctc
    14761 gcgcttctgg agaagaaaag gccatttcag cagaaagcac ttcatccact ggaaataaat
    14821 ggatctgatc ttgcggaatc acatgttgca gatcttctgc taactgattc gcataataca
    14881 aatttggcat cagaataatc atgtttttat ttgttttctt aaacgctcga gcaaatagaa
    14941 gtgttttcgc tgagcccgct aatcctgtga ttagttgtct aggcattttt tcttctaaat
    15001 tgctgaccca ttcttttact tgcggatctt ttccgactaa ttgaataata tccattcctt
    15061 ttcctccttc ctcaattgaa tcgattcatc gcatccacga acgaatgccc ttctatgaga
    15121 tattcgactg ctttaacaga ttgacacatg ctttcttcta tcaaaggctg attttctttg
    15181 ctgaaaggac taagcacatg ctggactact gtttttttcc cttctggcct gccgattccc
    15241 accttgattc gatcaaagac attcgtattc aaatgggaaa taatactttt gataccgtta
    15301 tgcccgccgg cacttccctt ttgacggagc cggatttttc ccactgccag atccaaatca
    15361 tcgtagatta cgactaattc ctctggataa atcccaaaat aagtcattag tgggccgact
    15421 gcacgaccag attcattcat gaatgtttgt ggtttgacta aaaggatctt ttctccatta
    15481 tgaaaaaatt cggcaacttc tgcttcgaac ggattttttt tgaaagtagc ttgatgctcg
    15541 cgtgcaattt tatccacgac cataaagccg atattgtgtt ttgtatattg gtatttggta
    15601 cctgggtttc ctaacccgac gatcattttc atttttgttt tccacctaat tctaaaaagc
    15661 agcacaaaag gctggactgc taattagcgg tccagtccat cgagcttttt tcttttttat
    15721 acttgtcaat gcatagtata acacaaaaca aggcgtttcg gctaaaagag aatcgttaat
    15781 aaatgataaa gaaattaaaa atttcttcaa agtttaatga aataattgat atataaaaat
    15841 agtgaaataa cgcacatgta agctttttca tataatacaa ccttgttatc tgttatattt
    15901 tatagccaaa tagcgtgaca tggggcttga gaaatgttag cataagcatg ggaaaaaaca
    15961 aatcaatgga aggattgata ctaaatgact gcagctgcag aaaaaaaaga tcatcaaaaa
    16021 gttattcttg ttggagacgg cgccgtaggt tctagctatg cctttgctct agttactcaa
    16081 aatatcgctc aagaagtagg tattgtggat atcaatactg ctaaaactga aggagacgca
    16141 attgacttgt ctcacgcttt ggcatttact tcacctaaaa aaatctattc tgctacttac
    16201 gcagacgcac atgatgctga cttagttgtt attacagctg gcgcaccaca aaaaccaggt
    16261 gaaactcgtt tagacttagt aaacaaaaac ctaaaaatca accgcgatgt tgtcacacaa
    16321 atcgttgaat caggatttaa cggtatcttt ttagttgccg ctaacccagt agatattttg
    16381 acttactcaa cttggaaatt ctcaggtttc cctaaagaac gcgtaatcgg ttcaggtacc
    16441 tctcttgact cagctcgttt ccgtcaagca cttgcggaat tagttgacgt tgatgcacgt
    16501 aacgtccatg cttacatctt aggtgaacac ggagattcag aattcccagt ttggtcacat
    16561 gcaaatgttg ccggcttgca aatctatgaa tgggtaaaaa acaacccaga aatcgatgaa
    16621 gaagctatgg ttaacctatt ctttggcgta cgtgacgctg cttacacaat tatcgagaaa
    16681 aaaggcgcta ctttctatgg tatcgcagta gcattagcac gtatcactcg tgccatctta
    16741 gatgatgaaa atgctgttct tcctttatca gtattcatga atggcgaata tggattgaac
    16801 gacatctaca ttggtgcacc tgctgttatc aaccgtcaag gtatccaaaa agtaatcgaa
    16861 attcctttaa gtgacagcga gcaagaccgt atggctgctt ctgctaagca attaaaagaa
    16921 attctagatg aagcttttgc taaactagac gctgaataat ctttgaacat gacggaaaac
    16981 atctattcgg tgttttccgt ttttttattt tatcaccctt atggtttcta aattttttca
    17041 taaagtagag ataaccttcg tcttttcctc taaattatgc tatcatgaga ccgttccacc
    17101 ctaaactttt acttaaggag ttgtcgatat gacaagatct tcgcatcgaa aaaaatcgaa
    17161 acatgttgct ttgctttcag ttcttggtgt tctcttagtg ctgatcagct tttataccta
    17221 tcgcagtaca tactacacaa agcatttttt gccgaataca gaaataaatg gaataaatgt
    17281 cagcaatcta acagtcgaaa aagcaaatga aaaattaaaa gaagcttatt ctgataaaaa
    17341 actggctatt aaagaagatg gaaaagtctg gcaggaagta ccaaaatccg agctcggcta
    17401 caaagatgat ttcacttcgg agctttctcc gatcctaaat gaacaaaacg gatggacttg
    17461 gggaatgact tatgtctcag cggcagaaaa acaagaaata gatcctgtca gtcaaaacca
    17521 ccaaaaacta gacactaccg ttcagacatt gacaactaag ctgactgatt tgaacaaaga
    17581 ccggacacct actactgatg caacgatcga aaaagcaggt gactctttcg ttatcaagcc
    17641 tgaagtcaat ggaaatacga tcgacgtcga tgctgcggtc aaacaattaa aaagtgcggt
    17701 aaactctggt aaagatacaa ttgaattgac tgaattcaaa gaaaaaccaa aagtaacatc
    17761 tgaagacagc tcactgaaag aacaactcgc atccatgaat gctatcgcaa atgtaaaagc
    17821 tacttacagt ataaacggtg agactttcca gatcccatca agtgatatta tgagttggct
    17881 gacctacaat gatggaaaag tcgatttaga tacagaacaa gtacgtcaat atgtaacaga
    17941 tttaggcaca aagtataaca caagtacgaa tgacacaaaa tttaaaagta caaaaagagg
    18001 agaagttacg gtcccagttg gcacttacag ttggacgatc caaacagaca gtgagacaga
    18061 agcattgaaa aaagcgattc tggcaggcca agattttact cgttcaccca ttgtgcaagg
    18121 tggcacaacc gctgatcatc cattgatcga agatacgtat atcgaagttg atctggagaa
    18181 ccagcatatg tggtattaca aagacggaaa agtggccctt gaaacagata tcgtttccgg
    18241 aaagccaact acacctacac ctgctggagt cttctatgtt tggaataaag aagaagatgc
    18301 aactttaaaa ggaacaaatg acgatggaac accttacgag agtccggtaa actactggat
    18361 gccgattgac tggacaggtg ttggtatcca cgattcagac tggcaacctg aatacggtgg
    18421 agacttatgg aaaacccgcg gttcacacgg ctgcatcaac accccaccaa gtgtaatgaa
    18481 ggaattattc gggatggtcg aaaaaggaac gcctgtattg gtcttctaac aaaaataata
    18541 tataaaaaga aagcacccgt acgattggta caggtgcttt ctttttatta tctaacaatg
    18601 tgctacttac atttcagcag cagtaaagac ttctgaaaca tcatcatcgt cttctagctt
    18661 atctactaga cgttccaatt gctctttttg ttcctcattt aaatcaatcg tcgtttgtgg
    18721 aatcatcgtc aattctgctt gcgccaaagt gaactctttt tccaactcat ctcgtacagc
    18781 agcaaaatct tctggtgcag tataaatctc gaatacttca ggagaagttt gcagatcttc
    18841 tgcccccgcc tctaatacat cctcaaacat cgtctcttca tcaacagtca acccatcgcg
    18901 ttcaatagcg atatatcctt tgcggtcaaa caagtaactg actgaacccg tttctcccag
    18961 tgatccaccg tttcgagtaa aagctactcg gacatttgtc gctgtccgat tacggttatc
    19021 tgtcaatgca tggactaaga ttcctactcc acccggacca tatccttcat acgtgatttc
    19081 atcgtaattc gtttcatcta cggcactcgt agccttttta atcgcccgtt ccacattgtc
    19141 gttaggcata tttgcagctt ttgctttgtc cacaacaagc cgcaaagctg gattcataga
    19201 tggatcagga ccgcctgctt tagctgccat ataaatttca cgagaaagtt tctgaaagac
    19261 tttccctcgt tttgcatctt gggcattttt tcttccttgg atattatgcc atttactatg
    19321 tcctgacatg atttttcctc ccttgttctt ttgagaatac acttctattc tagtactatt
    19381 tcacaaaaaa acaaagccac agccaatcta taaacgcttc ttgaatgccg caaaaagcac
    19441 acataatatc acagcagtca gagcaccgat cgaatccaac atgacatctt ggaacatagg
    19501 tgttcgtcca ccggtcatca tctgatggaa ttcgtccaaa cctgcgtacc ctgttgctga
    19561 aagccaagca aggatcgctg tcagccacca aatttttagc ttggggaaaa cacctaaaaa
    19621 caagctgcct cccaaaataa aataggtacc gaaatgtgcg cccttcctta aaaagaactc
    19681 taaaaatttg gcgtagccca gatggtcgat actcaccata cttccaccat aattaaaacg
    19741 tatacccgct aatgtgtctt taaatggctg attaggcagc cagctttcta atcgcgaaac
    19801 ttgtgattgc tgtccgtaag tttgagaaga actgtaaaat aagattataa cgactatcag
    19861 tgcgattact aaataaatat ttccgttttt catttgtttt tttgaagaat tcataagtca
    19921 cctctgtatt ttatcttaga ctttttttga agaaacaaca atcattttca tagagacttc
    19981 ttccataaaa aagtgctata cttaattttg tattacaata tgaagggagt tttttcatga
    20041 gttattcatt aaattggaat ctagattcta ttttttccgg aggcagtcat tctgatgctt
    20101 tgaaccaacg gatgaagcaa ttggaagacc aaatgaacga gtattatcac cgcgtaacaa
    20161 aatggtcacc tagttctgac aatgcagagc agttaaacgc tattcttcaa ttgcaagaaa
    20221 cgattacgaa tggttttacc caatgcagca gctatattac tgcattgctt tctgccaatg
    20281 tcaatgattc agatgcaaag gtcttatcag gcaagcttta cgcaatgctg cctcgtctgc
    20341 aatcagccga aactgtattg tcaaagaaat ttgcagaaat atctgacaat gattggaatc
    20401 agatgctttc tcaatcttcg tttgaaacaa ttgcttttcg tttaaacgag attcgtcgtg
    20461 atggctcaca attattatca gaagctgaag aaaacattat aaatacgctt tctttagatg
    20521 gtctaaatgc ttggagcagt cattatgata cgattgtagc cagcatttcg attcccttcg
    20581 aacaagatgg acaagtagtc gaattatctg ctggacaagc cttcaataag atgatgggcg
    20641 atccagatcc aaaagtcaga gaaacacttt tcgcagcatg ggaagaagcc tggaaagaaa
    20701 aggcacccct cttcacggat actttgaatc atttagatgg ttttagactt tctgactatg
    20761 aacttcatgg tgtcacagat tttctacaaa aaccattaga gtacaatcgg ttgaagaaag
    20821 aaacattgtc tgtcatgtgg gatacaatcc agaaaaacaa acaacccctt gttaattact
    20881 tgactagaaa agctaatctt ttcggtaaag aaaaaatgga atggcaagat caagatgcac
    20941 ctattatttt aggcgatttg aaagaaaaaa cctttagttt tgacgaagca gcagcattta
    21001 ttatcgaaaa ctttaacaaa ttcagtccaa aaatggctaa atttgcccaa tctgcttttg
    21061 aaaagagctg gatcgaagca gaagatcgtc ctgggaaacg tcctggtggt tattgtactg
    21121 agttaccaga aacaaaagaa tcaaggatct ttatgaccta cagtaattct gttaatgagg
    21181 ttgcgacact tgctcatgaa ctaggacatg ctttccatag cagcacgatg tgggatttac
    21241 cttctttaaa tcgcgaatat gccatgaatg tcgcagaaac agcaagtaca ttcgcagaac
    21301 tgatcgtagc cgatgctaca ttaaaagtcg caaaaacaaa agaagaaaaa atcaatttgc
    21361 tagatacaaa actgcaaaat gctttagcta tgttcatgaa tatccattcc cgattcattt
    21421 ttgaaaatcg attctatgaa gcacggcaag aaggattagt ctcagaagat aagatcacag
    21481 agatgatggt tgaggcgcaa aaagaaggct atcaaggagc tttagctact taccatccat
    21541 atttctgggc tgcaaaattg catttcttta ttgatgacgt accgttttat aacttcccat
    21601 acacatttgg ctacctcttt agtttgggca tctatgctta cgccaaccaa aagggagcaa
    21661 attttgaaga agaatatatc catttactac gcgatactgc ctcaatgacg acggaagatc
    21721 ttgcgaagaa acatttgaat gtcgatctta cccaaccaga tttttggcaa gcaggtatcg
    21781 acatggtgac tgaagatatc caaacgttca tggaattgac agaagaattt atctgatttc
    21841 tattttatca caataaaaaa agagttagtc cagaatgggc taactctttt tgattaatca
    21901 ttcttacatg ctgacagcaa agtcaggggc aaaccattga actgaaccaa cttttacgct
    21961 ttcgcctggt tgaggagcat ggatatattg tcctccgcct aaagcaatcg ctacgtggta
    22021 agttccgcct tgtgaccccc agaataacaa gtctccagct tttgcttgag aaactgaaat
    22081 ttttgttcca gctgattctt gaggaactgt ccaaccaccg atgtcacgac cagttacttg
    22141 catgtaaaca tagcgtgtga accctgagca gtcaaagcca cttggatctt taccgcccca
    22201 aacatatgga gtaccaatat atttgtaagc ttctgctacg attgctgcac cattcacact
    22261 tccagaagga gctggagcag gtgttacagg ttttggttgt tcaggtgttg gtgttggcgt
    22321 tggagccgga gtactacttc ctgtgccgtt tcctgtatca acactttgat ctgttgatgg
    22381 tgttgtaggc gcaggtgttg ttgattcttc tgttgttgaa gattcaggca ctgttgttga
    22441 ttcttctgtt gctgaagatt caggcactgt tgttgattct tctgttgctg aagattcagg
    22501 cgctgttgtt gattcttctg ttgctgaaga ttcaggcgct gttgattctt ctgttgaaga
    22561 ttcaggtgtt gttgattctt ctgttgctga agattgtgta gctgatgatg attcttcagt
    22621 cattgaagat tgtgtagctg atgattcttc tgttgctgag ctttgtgcag tagatgaagc
    22681 ttgagtagct tgtgcttcag cctctgcttg ttcgcgtgct tctttttcag ctttttcttg
    22741 tgcagcttgt tgacgagctt gttctgctag acgttgttgt tcacgaatac gagcttgctc
    22801 tgcttcagct tctgcttttt gacggttcaa gtcagctttc ttgtcttcag cagttgcttg
    22861 ttcagcagct aatgaagttt tcagtacgtt caaatcagct tgtttagata aaagatcgcc
    22921 tttttgagat tccaaagcag cttggttttc agctaattct tttagtttag catcgttttc
    22981 agcttttttg tcttctacag cttttttatc ttgtttttgt tgttccatca agtcgttgtt
    23041 tgcttttacc atcgttgtca ttgcttgaac gcgtccaata gcatctgcta atgaatcagc
    23101 attcaataca gcatcgatgt agtttgagct tgtgttgctt acttgtgctt cgcgtgcttg
    23161 tttttggatt gtatcttcac gtttttcaat acgttcttgt aaatctgcaa tgtcttttac
    23221 taattgtgca gattcttgac gcaatgtatc ttgttttgct aataggtctt gtgcttgtgt
    23281 gttgatttca gaaacttggc tttctaaggc atcgatttgt gattgtgcat ctgcttgttg
    23341 atttttcaag tctgcaatct tttgatcttg ctgttgtatc tgagaatcaa aatcgtccgc
    23401 cgcggcagcg atcggtgatg ctacggcagt caaggtcatt gaacatacca ttactgctga
    23461 tattaaactc tttttcactc ttcattcctc cgactggctt aatttaataa atatctaaat
    23521 atatttaagt ctttcttaac gatataaaaa ttgtaccata gcagtgtgtc aacgatataa
    23581 tagttatgtt acaaaaatgt ttcaaaacaa cttttaaaaa tgacaaaact gttcgtttca
    23641 gaaaatgatc atgaaaaagc cgcgttaagt ctag
//
LOCUS       DAZKCY010000032        22837 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000032 DAZKCY010000000
VERSION     DAZKCY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.032"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..369
                     /locus_tag="ACG8XC_001167"
     CDS             1..369
                     /locus_tag="ACG8XC_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP9190879.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="ACG8XC_001168"
     CDS             549..1490
                     /locus_tag="ACG8XC_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP9190880.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="ACG8XC_001169"
     CDS             1559..2290
                     /locus_tag="ACG8XC_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9190881.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="ACG8XC_001170"
     CDS             2434..4614
                     /locus_tag="ACG8XC_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP9190882.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="ACG8XC_001171"
     CDS             4634..5116
                     /locus_tag="ACG8XC_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFP9190883.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="ACG8XC_001172"
     CDS             5132..5893
                     /locus_tag="ACG8XC_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP9190884.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /gene="murC"
                     /locus_tag="ACG8XC_001173"
     CDS             6024..7358
                     /gene="murC"
                     /locus_tag="ACG8XC_001173"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP9190885.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="ACG8XC_001174"
     CDS             7665..8150
                     /locus_tag="ACG8XC_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFP9190886.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="ACG8XC_001175"
     CDS             8143..8826
                     /locus_tag="ACG8XC_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9190887.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="ACG8XC_001176"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="ACG8XC_001176"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP9190888.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="ACG8XC_001177"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="ACG8XC_001177"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFP9190889.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /gene="coaE"
                     /locus_tag="ACG8XC_001178"
     CDS             12442..13071
                     /gene="coaE"
                     /locus_tag="ACG8XC_001178"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP9190890.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="ACG8XC_001179"
     CDS             13123..13455
                     /locus_tag="ACG8XC_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9190891.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="ACG8XC_001180"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="ACG8XC_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP9190892.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="ACG8XC_001181"
     CDS             14281..15654
                     /locus_tag="ACG8XC_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP9190893.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="ACG8XC_001182"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="ACG8XC_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFP9190894.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="ACG8XC_001183"
     CDS             16709..17149
                     /locus_tag="ACG8XC_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9190895.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17293..17994
                     /locus_tag="ACG8XC_001184"
     CDS             17293..17994
                     /locus_tag="ACG8XC_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFP9190896.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17991..19112
                     /locus_tag="ACG8XC_001185"
     CDS             17991..19112
                     /locus_tag="ACG8XC_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFP9190897.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19127..20359
                     /gene="pepT"
                     /locus_tag="ACG8XC_001186"
     CDS             19127..20359
                     /gene="pepT"
                     /locus_tag="ACG8XC_001186"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP9190898.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20491..21399
                     /locus_tag="ACG8XC_001187"
     CDS             20491..21399
                     /locus_tag="ACG8XC_001187"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9190899.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21434..21706
                     /locus_tag="ACG8XC_001188"
     CDS             21434..21706
                     /locus_tag="ACG8XC_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFP9190900.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21717..22763
                     /locus_tag="ACG8XC_001189"
     CDS             21717..22763
                     /locus_tag="ACG8XC_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFP9190901.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7697 a         3981 c         5013 g         6146 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aatattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 atacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata attatctaga
    17281 aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt
    17341 gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca
    17401 ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat
    17461 gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt
    17521 gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt
    17581 atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc
    17641 ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg
    17701 caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat
    17761 gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg
    17821 ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag
    17881 cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt
    17941 caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca
    18001 gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag
    18061 gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga
    18121 cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga
    18181 ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag
    18241 aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca
    18301 tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg
    18361 agagttattt agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg
    18421 tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg
    18481 attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg
    18541 cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg
    18601 ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt
    18661 atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag
    18721 gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag
    18781 aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga
    18841 agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa
    18901 gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg
    18961 ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta
    19021 tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg
    19081 aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct
    19141 tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac
    19201 gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga
    19261 gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc
    19321 aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga
    19381 ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat
    19441 cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa
    19501 ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa
    19561 atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc
    19621 tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa
    19681 attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg
    19741 tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa
    19801 tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt
    19861 tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt
    19921 ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga
    19981 tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt
    20041 gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat
    20101 gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga
    20161 gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc
    20221 ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt
    20281 cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga
    20341 attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat
    20401 gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct
    20461 cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa
    20521 agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa
    20581 aagcagccag aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa
    20641 ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg tggagacggc
    20701 acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta
    20761 cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa
    20821 gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat
    20881 gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc
    20941 agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta
    21001 ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga
    21061 aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac
    21121 gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt
    21181 ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc
    21241 attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact
    21301 cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga
    21361 ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt
    21421 tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt
    21481 gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg
    21541 gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt
    21601 gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc
    21661 tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt
    21721 cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg
    21781 ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta
    21841 ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag
    21901 ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt
    21961 tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga
    22021 tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga
    22081 cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg
    22141 tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg
    22201 gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg
    22261 ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat
    22321 gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc
    22381 aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt
    22441 atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca
    22501 tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg
    22561 gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag
    22621 tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg
    22681 tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag
    22741 agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat caaatcaaag
    22801 aaaaacgtta tggataagat aattttacgt ttgtttg
//
LOCUS       DAZKCY010000033        22248 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000033 DAZKCY010000000
VERSION     DAZKCY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.033"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..837
                     /gene="purR"
                     /locus_tag="ACG8XC_001190"
     CDS             19..837
                     /gene="purR"
                     /locus_tag="ACG8XC_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFP9190902.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1340..2713
                     /gene="glmU"
                     /locus_tag="ACG8XC_001191"
     CDS             1340..2713
                     /gene="glmU"
                     /locus_tag="ACG8XC_001191"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP9190903.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2807..3778
                     /locus_tag="ACG8XC_001192"
     CDS             2807..3778
                     /locus_tag="ACG8XC_001192"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9190904.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSIGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3784..3886
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3952..4521
                     /gene="thiT"
                     /locus_tag="ACG8XC_001193"
     CDS             3952..4521
                     /gene="thiT"
                     /locus_tag="ACG8XC_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFP9190905.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4602..5375)
                     /locus_tag="ACG8XC_001194"
     CDS             complement(4602..5375)
                     /locus_tag="ACG8XC_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190906.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5642..7342
                     /locus_tag="ACG8XC_001195"
     CDS             5642..7342
                     /locus_tag="ACG8XC_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9190907.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7312..7530
                     /locus_tag="ACG8XC_001196"
     CDS             7312..7530
                     /locus_tag="ACG8XC_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFP9190908.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7550..8440
                     /locus_tag="ACG8XC_001197"
     CDS             7550..8440
                     /locus_tag="ACG8XC_001197"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP9190909.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8458..8742
                     /locus_tag="ACG8XC_001198"
     CDS             8458..8742
                     /locus_tag="ACG8XC_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190910.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            9229..9843
                     /gene="gmk"
                     /locus_tag="ACG8XC_001199"
     CDS             9229..9843
                     /gene="gmk"
                     /locus_tag="ACG8XC_001199"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9190911.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            9848..10153
                     /gene="rpoZ"
                     /locus_tag="ACG8XC_001200"
     CDS             9848..10153
                     /gene="rpoZ"
                     /locus_tag="ACG8XC_001200"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9190912.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            10406..12814
                     /gene="priA"
                     /locus_tag="ACG8XC_001201"
     CDS             10406..12814
                     /gene="priA"
                     /locus_tag="ACG8XC_001201"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP9190913.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            12829..13320
                     /gene="def"
                     /locus_tag="ACG8XC_001202"
     CDS             12829..13320
                     /gene="def"
                     /locus_tag="ACG8XC_001202"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9190914.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            13313..14251
                     /gene="fmt"
                     /locus_tag="ACG8XC_001203"
     CDS             13313..14251
                     /gene="fmt"
                     /locus_tag="ACG8XC_001203"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP9190915.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            14251..15609
                     /gene="rsmB"
                     /locus_tag="ACG8XC_001204"
     CDS             14251..15609
                     /gene="rsmB"
                     /locus_tag="ACG8XC_001204"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP9190916.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            15622..16362
                     /locus_tag="ACG8XC_001205"
     CDS             15622..16362
                     /locus_tag="ACG8XC_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFP9190917.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            16359..18428
                     /gene="pknB"
                     /locus_tag="ACG8XC_001206"
     CDS             16359..18428
                     /gene="pknB"
                     /locus_tag="ACG8XC_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFP9190918.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            18593..19492
                     /gene="rsgA"
                     /locus_tag="ACG8XC_001207"
     CDS             18593..19492
                     /gene="rsgA"
                     /locus_tag="ACG8XC_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFP9190919.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            19505..20155
                     /gene="rpe"
                     /locus_tag="ACG8XC_001208"
     CDS             19505..20155
                     /gene="rpe"
                     /locus_tag="ACG8XC_001208"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9190920.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            20156..20794
                     /locus_tag="ACG8XC_001209"
     CDS             20156..20794
                     /locus_tag="ACG8XC_001209"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFP9190921.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            20949..21803
                     /gene="mreC"
                     /locus_tag="ACG8XC_001210"
     CDS             20949..21803
                     /gene="mreC"
                     /locus_tag="ACG8XC_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP9190922.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            21807..>22248
                     /gene="mreD"
                     /locus_tag="ACG8XC_001211"
     CDS             21807..>22248
                     /gene="mreD"
                     /locus_tag="ACG8XC_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP9190923.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPT"
BASE COUNT         7413 a         4000 c         4835 g         6000 t
ORIGIN      
        1 taaaaggagt tggaataggt gaaagtacgt cgcagtgaac gtctagttga catgacacag
       61 tatttattga atcacccgca tgaattgatt tccttaacaa gctttgccga acgttatgaa
      121 tctgctaaat cttctatcag tgaagatttg ggaatcatca agaaaacatt taaagaacga
      181 gggtatggaa ccttggaaac aatcccgggt gctgcaggtg gtgtattgtt catccctgaa
      241 atttcatatg aagaggcgaa aaaatatatt gaatctctag cagaacgtct atcagaacaa
      301 gaccgtctgc ttccaggagg atatgtttat ctctctgatt tgttaggaga acctgaactg
      361 ctgcgtcaag ttggaagaat cattgcttct aaataccttg gtgaaaaaat cgatgctgta
      421 atgactgtag ccacaaaagg cgtacctatt gcccaagctg tgtcctatta tctgaatgcg
      481 ccattcgtta ttgtacgccg ggactcaaaa ataacagaag gatcaacagt aagtgtcaac
      541 tatgtgtctg gatcttctga aagaattgaa aagatggaac tgtctaagag aagtttgaaa
      601 cgtggttcac gtgtccttgt agtggatgat ttcatgaaag gcggcggaac agtcaacgga
      661 atgaagagct tgattgaaga attcgaatca gaacttgttg gcatcactgt atttgccgaa
      721 tcaaaattca acggtcatcg cgccatcgat gattatacgt cactgcttta tgtcaaagat
      781 gtagatactc aaacgaaaaa tattacggtt gtgccaggaa attatttttc agaataagag
      841 gttgtgaaaa agccgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcca
      901 cattttttca taaatttggc ttaataccga gaaactggct tttgaacacc gtttatctag
      961 aggttgtgaa atcagcgtcc tgtcctgagt attagctcaa taaacggaaa aattgctcct
     1021 catattttgc cgtttattga gctaataccg aaggtcagct gattgaaaaa cgtttaatga
     1081 agaggttgta agcctaccgt ttaactctaa gtcacaagaa acgatttcaa aaaacagctt
     1141 ttcatgtttt atgagtagtg tgacttgtga ccgaggagtt ggcaggagag caccggttac
     1201 caaatagata ggcccgttat ggtctatcta tttttttatc cagaaaacgc tgggataaaa
     1261 gaaaatcact tgttttgttg aaaaaatggg tatactggga taaactgaaa tcagcaaact
     1321 atatttagga gtgacgcagt tggacgcacg ttatgcaatt attttggcgg cagggaaagg
     1381 tacccgcatg aagtcaaaat tatataaagt tttacatccc gtttctggta aacctatggt
     1441 agaacatatc attaatcggg tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg
     1501 acacggagca gaacaagtta aagcacaact aggagaacgc agtaaatatg cgttacaagc
     1561 agaacaattg ggaacaggac atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa
     1621 aggaacaacg ttagttatca gtggcgatac accattattg actactgaaa cgctgaacaa
     1681 tctttttgaa tatcaccaag gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga
     1741 aaacccaaca ggatatggac gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt
     1801 ggaacaaaaa gatgcaacac cagaagaagc tcttgtacaa gaaatcaata caggaactta
     1861 ctgttttgac aacgaagctt tgttcgatgc gttaagcaaa gttggaacaa acaatgctca
     1921 aggtgaatac tatctaacag atatcatcga aatcttaaaa gaagaaggcc atacagttgc
     1981 tgcttatcaa acggatgatt ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc
     2041 taaagcaaat gagatcatga gaaagcgaat taaccagatg catatggtca atggcgtaag
     2101 ctttgtcgat tcagcaacga catacatcga tgcaggcgta gaaatcggtc cagacacatt
     2161 gatcgaagca ggtgtacaaa tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg
     2221 gtcacattca aaaatcgtag acagtaggat tgaggatcac gtcgtcattg aaaactcagt
     2281 cattgaaagc agccatgtca agaaacatgc agatgtggga ccttatgcac atttacgacc
     2341 aaaagctgaa attggcgaaa acgtccatat cggaaacttt gtggaagtga aaaatgctca
     2401 aatcggcaaa ggaactaaag tcggtcactt gacttatgtt ggtgatgcta cgctgggaga
     2461 agaaatcaat gtcggatgtg gtgttgtctt cgtcaactat gatggaaaga acaaacatca
     2521 tacgactgtc ggcgaccaca gctttattgg ttctagcacg aatatcatcg gacctgtcga
     2581 ggtagctaaa aacagttcga ttgctgccgg gtcaacaatt acagacaata ttccagaata
     2641 tgcgctggca attgcgagag caagacaagt caataaagaa ggatatgcaa aaaaactccc
     2701 ttatctaaac taagactttt gcaaaatcac ttgatttatt taaaaaatat gactactatt
     2761 tattaggtag aattctagag ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta
     2821 ctttgaccca agattaaaaa tttttgcgtt aaattccaat cgcccattag cagagaaaat
     2881 tgcagcagca gtcggcgtcg agttaggaaa atcatctgtt actcaattta gtgatggaga
     2941 aattcaagta aacattgaag aaagtattcg tggatcgcat gtctatgtta ttcaatcaac
     3001 gagcagccca gtcaatgata acttgatgga gttgctgatc atgattgacg cactgaaacg
     3061 agcaagtgca aaaacaatca acgtagtcat gccttattat ggctatgcgc gtcaagaccg
     3121 taaagcacgt gcacgggaac caatcacagc gaaattagta gcaaacatga ttcaaaaagc
     3181 tggtgcaaca agaatgctga ctttggacct tcatgcagtc caaatccaag gtttctttga
     3241 tattccagtc gatcatttga tgggtgcgcc acttattgcc aattatttct tagaaaaagg
     3301 aatcaaaggc gatgatgttg tcgttgtttc accagaccat ggtggtgtca cacgggcacg
     3361 taaattagcg gaatttttga aatctccgat tgcgatcata gacaaacgcc gtccaaaagc
     3421 aaatgttgca gaagtaatga atatcattgg acaagtcaaa ggcaaaactt gtgtactgat
     3481 cgatgatatg atcgatacag caggtacgat cacattggct gccaatgctt tgaaagaagc
     3541 tggtgcaact agtgtgtatg cttcatgtac acatccagta ttatccggcc ctgctttaca
     3601 aagaatagaa gattcagcta tcgagcgtct agttgttaca gactcgatct atcttccaga
     3661 agatcgtaaa atcgacaaaa tcgatgaagt aagtattggc ggactaatgg gagatgcaat
     3721 caaacgtatc catgaaaaca aaccagttag tccattgttt gaaacaaaaa ataaataata
     3781 aataaactca caaaggggag tccaattggg ctgagattga atctatttct aaacccttcg
     3841 tacctgtatc ggttatgcga gcgtaggaat tgtgaataaa cagctgtttg actattttcg
     3901 aaagctgctt cctcctttgt gactttttta ttcaaaggag gaagtttttt tatgggaaaa
     3961 aatagagttt gggtagaagg aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt
     4021 ccgatccaga tcggcagcag tttttctatt tcattagggc agattccatt gactcttttt
     4081 gcgttaagaa gagggacaaa ggcagggatg ctggcaggtt tcatttgggg catcctacat
     4141 tttccgttgg gacaagttta ttatttaagt gtcgttcaag tgttgattga gtacccgatt
     4201 gcctatactt ttgcaggcgc agcagggctc atggcttcaa atgtccaaaa aggctatgta
     4261 gacgataata cgaaacaaat tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt
     4321 tccagatatt tttggcattt tattgcagga gtggttttct ggggagcgta tgcactatgg
     4381 gggatgaatc cttggctctt ttctttcgta atgaatggtg ccagcgggtt gagtacagct
     4441 attgtaacga tcatcgtttt attagggatc caacaaacga ctccttcgat ttttgtgcca
     4501 gataaacgac aggagctgta aataaaaaag aagcaagaaa cttgagacag aatcatgtcc
     4561 tagtttcttg tttccttttt attttttgat aagaaataga tctacttcaa tggcagataa
     4621 gaaagatcgg catcttctac caattgccag tgtcgatcgg tgacagagta gattaaagta
     4681 gcgatgattt cctcttctcg ataccgggtg ccttttaatt tccaaatata atcttcaatc
     4741 acatagattg ggatacgata tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg
     4801 atcccttcct gcatcaaatt acgcatttct ttcaataatt ccacactgat tttttcaaca
     4861 ggtgtttctt ttttgaaaaa agattttttt acattgatct tatctgacaa gatcgaactt
     4921 aatttactag caaaaatcct ataaaattca tctaaatagc gataataaat ccgattcagc
     4981 tgatcattaa gatcgaagta tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga
     5041 agatgtgttt tcatatgacc aaaatataat aattctgaaa tttctaaagg tgtcaattct
     5101 ttcagcatcg ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc acgcctgtta
     5161 cgagaagagg aaaagaaacg ctgaatattt tctgtaccac ggattacttt cagccctgta
     5221 tgggattcat attctccagc agaagcagat ggatcaagca agagcaagtt ttgaggaaaa
     5281 tgcacgatac tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac agcattactt
     5341 gtggtatcaa tatgtacata aactgactca gtcatcatgt tcctcctctt atttcctctt
     5401 aatctctttt attttacaac atttgcatta aaaaagcttg tttcttttat tttaaaattc
     5461 cattacaaaa tcacgttttc attaaaaaca ctctcatttt caattaattt ttgtgatttt
     5521 tgtatttttt tatttaaagg aggttttatg aattgttttc ttatggaatc tacaaagtcg
     5581 aatacgagac tcatgacctt tttatgacgt actggtatac ttgaagaaaa aggagagcgc
     5641 gatggagata aaaaaagttt acttttcaca gaaaatgtcg ctgtattcgc ctatccagca
     5701 agcaaaacaa ccccttccat tttttcaaag tcatcaagaa agtagagacg ctttttggcg
     5761 ttctgcaaaa aagcaatgtg cttttaaagc agtatcgaaa aaatataccg tctattttac
     5821 tattgctaaa agtagaggag atgttatttt taataatctg ttgattgaag gtgaaacttt
     5881 tgaatggaaa aaattttttc ggtatatgga agcttgcgct cgttttttta ttaaagaaaa
     5941 ctgttgtttt ttatttcaat ctcctctatc agaagaatgg ttgcgtattt ttcataaaaa
     6001 cggctatcaa ggaaccacac aattaaataa aaagctggtc tatcatacag cacttgtttt
     6061 aggtggagga ggagcgcacg gtgcttatca gattggtgtg tggcaggcgc tgaaggaaca
     6121 tgatatcaac tttgagatta tcacaggtac ctcggtaggt gcgttgaacg gcgcattgat
     6181 tttacaagga gatatgaaaa aagctgtgaa tttatggaaa aagctgtcga caagacaagt
     6241 gcttgcgtta ccagaaatgg ctgcagttga agaccttcgt gaacgcttct atcgagaaag
     6301 acgccaaatg acgaaaacag cactaattga aggcggggtt tcttctgctc cattggaaaa
     6361 gatggtcaaa gacaatttgg atttgtcctt actcaaacat aatcccaaaa tacgtttata
     6421 tacggtgtct actaaactgc cagaactagc ggaagtagtg actgatatcc aacagacaaa
     6481 aacagaagaa ataccagatt ggatactcgc ctctgcttca ttttatcccg ctatggctta
     6541 tcgaaagatc ggaaaaaata aatatgcaga cggtgggtat cgcaacaaaa tacctattga
     6601 tattgcgatt aacaaaggag caacagaggc atttgtcgta gatgtgcaag ggccaggccc
     6661 agcgaaaaaa attcgtatgc cagacatttt tatccattgg aaatgtcaaa cattgtggac
     6721 actaggcagc tttctgcttt ttgatagcca aaggaaccaa ttaaatttac aattaggcta
     6781 tctggaaatg aaaaaacgat taggctatta ctatgggaac tggtatacat ttcattctgt
     6841 taaacctgca agaacatact ggcgaggatt tttgtcttac ttaacaaaag aaattcagtt
     6901 agaattatct tttttcaaaa gcataaaatt ttggcaaaaa ctgcgtaatt tatataagaa
     6961 cagggtcgta ccagagacat gcggattggc gatgctagaa cttttagcaa aaaaacattt
     7021 tttactgccc aatgaaatct atcaagtaga catgatgatt caaatgatct gccaacaaga
     7081 tataaaaagt atgccggatg atttattggc acaaattggt caattaagta ctgaagaatg
     7141 gcgaaggtat cgaaaatacc agcaaaaaat acaaaatgag cgcacgaaaa ctgtttattt
     7201 tgattatctg ctacaaggaa aacgcaagga tcgtttgcag catgaattgc ttaagcaacc
     7261 agttgacaca ttactgatcc tatatttata ttatctcaag gaggaacaac catggcacaa
     7321 gaattttcat atgaaatcgt agaagaaatc gctatattgt cagaaaacaa caaggggtgg
     7381 cgtaaagaac tgaacctagt cagctggaat ggtcgcccgc caaaattcga tcttcgagat
     7441 tgggcgccag accatgaaaa aatggggaaa ggcctgacat tgacgaacga agagtttgaa
     7501 caactgcaaa aagcaataga aaatatgtaa tgaggaagga ccctgtcgaa tgaaaagtat
     7561 gactggtttt ggcaaagcca tccgcgaaac aaaagcgtat cagttagata ttgaaataaa
     7621 aagtgtcaac cagcgttttc tagatgttca aatacgaagt cctaaaatcc tgaactttgt
     7681 tgaaaacgat tttcgtcagc ttattaaaca gcagttaagc cgcggtcgaa tcgaagtatt
     7741 tgtgaatcta gcttatattg gcaaaaacga aaaacagatc gtcatcaact gggaattgat
     7801 tgattcactt atcactcaat tgacagacgg aattcaacaa cgatacggta gtcaaaatca
     7861 agtgtcaatt agtcggatgg cggaacgctt cgcgctggac gaaaacttcg taacggtaga
     7921 agaaaaaccg gaagagagca tggaagaatt ggcacagtta gctctagata cattgaaaag
     7981 cgcacttgat tctattgagg cgagtcgaga aaaagaaggt caagctttag ctttagtgtt
     8041 gaaaaagaat actgaagaat tcaaagaagt actaaaaaaa ctcgatagtt ttgtagaaat
     8101 ctatgaaaag gaatttcaaa taaggtatca aaaaaagctg gaggaatttc ttggagcgaa
     8161 agtcgatgag caacggttgt tgacggagct agccatcttg ttggaacgcg gagatattca
     8221 cgaggaactg gatcgtctgg cgatccatgt acaaaaacta gatgaactta tcgaagcaca
     8281 atgtcctgta ggtcgggaat tagatttcct gatccaagaa atgaatcggg aaatcaacac
     8341 gatcggttcc aaatcaaatg caatcgaaat taaaaatcaa gtcgtccagt ctaagacgat
     8401 cttagaaaaa atcagagaac aagtacaaaa tattgaatga cgaaagaggt agagataatg
     8461 aaatattatg acgtaacgtt ccacgaatta agtggaaaaa ctgtggttaa acgggatatc
     8521 ccttctgaaa aaaacggctt tgacgtatgg aaagatgcct gtgcagacta taatgagaac
     8581 gaactgttta ttttgatcaa cgacggtgct tacgtgacga tgaatcggaa atttattgtt
     8641 cgaattgaca cagaagaggt agaagatccg acagaaaaag cacgttctcg aaaagatgag
     8701 atcatgggtg tagtgaatac tttgtccaat atgggctttt agcaaggatg ctgaacaaag
     8761 aaatacaata aataatcaga attggatttc ttcgcttagc ttctttgctc taggaataca
     8821 tttaaaggtt gtgacaaaat tcgtgtcaca atcttttttt attttgaata agtgatgttc
     8881 aaaaaccatt ttctcggtat taagccaaat ttatgcaaaa atgtgagaaa ctattttcac
     8941 aaaatttttc ttcatactcg aaactaatcg gcttcttcac gacctcttat acacggcgtt
     9001 caaaagcttg catctgcgat aataacccca accaaacgaa aaacatgaga agctgtttat
     9061 gtttggctgt tcttattact tgatgcagga cagctttttt acgacctctt ggtacagggt
     9121 agctttttta ccaatttcta gctataatta aagataacct tgaaagttgc agaaaaaaag
     9181 tgcaaaataa acgataagta caaaaagact agttgaaagg acgtcgtcat gtcagagcgt
     9241 ggattattaa tcgtgttgtc tggtccatcc ggtgtaggaa aaggcacagt acgaaaagca
     9301 atttttgaga gtgaggataa tgattttcag tattcgatct caatgactac ccgtaaaatg
     9361 cgtgaagggg aggtcgaagg cgttgattat ttcttccgaa gcaaagaaga gtttgaatca
     9421 atgatcgaag ctggagagat gctggaatac gcagaatatg tcgggaatta ttatggaact
     9481 cctttgtcat atgtcaacaa gacattggat gaaggaaaag atgttttctt ggagattgaa
     9541 gtccaaggag caaaacaagt caaagaaaaa gtgcccgatg gtgtattcat ctttttgact
     9601 ccgccagatt tagcggaatt aaaatcacgg atcgtaggcc gaggaacaga tgcagatgaa
     9661 gtaatcgaag aacgaatgaa agttgcaaaa gaagaaatcg aaatgatggc tctttacgac
     9721 tatgctgttg taaatgatga agtgccgcta gcagttgaac gaatcaaaaa cattattgcc
     9781 agtgaacatt ttcgagtaga tcgcgtaatc ggtaaatata ttaaaatgtt aaaggagatg
     9841 tagtcttatg atgttaaaac cttcaattga ctcactatta gatcgtgtaa attcaaaata
     9901 ttctctagtg attttagcaa gtaaacgcgc gcatgaattg gatgcaagtg cacagcctac
     9961 tctagattct tttgattcag taaaaagcgt cggtcaagct ttagaagaaa tcgatgcagg
    10021 aaatgtagtc aatgatccac atcctgaatt aaaacgtgaa cgtttgaaga tggaagaaga
    10081 ggaacgtcaa gcacaaaaag agcgtgaaca acacgaattg gaatcacgta tccgtgaaga
    10141 gcaaaaaatg taataaagaa aaatagagcg aacatctaaa aagaaaagca aacccagctg
    10201 tttccttttc aaacattcgc cacttattgt ctggtagaaa aggctgtgtc tgaacttttt
    10261 gacacagttc ttttttttct tatctgctgt taattttttg ttaggatttg gcagaagata
    10321 agacaaatcc atcgatctct agtaaaatag gcaaggaact ttttgtcgct tgataagaaa
    10381 agacaaaaaa aggaggaaac gaaccatgac attaacagct gaagtcattg tggacgtacc
    10441 tactatgcag accgatcagc cattcactta tttggttccc tctgaaatgg aaacagcttt
    10501 gcaagtcggg atgcgagtag aagtcccttt tggtaatggc aatcgtcatg tccaaggatt
    10561 tgttatggct atccaaaaat cagaagaatc agctaatcca tctttgaagg cagttattcg
    10621 tttgctagac cttgctccgg tggtcaatga agaattactt tcattagctg attatatgaa
    10681 aaaaattact tacgcattca aaatcacttg tctacaaacg atgcttccga gtgtgatgaa
    10741 agcagaatat gataaattga tctatccttt ggcagacaca ccagaagttc aggtattgtt
    10801 tgatcagcga gaagtgattt cctggaagga agcagaagaa gaaggcagcc tttctcaatt
    10861 gattcgctgg cgccaagagc aattagtaga tatcaaatat gaagtccata cacgaaataa
    10921 agtaaaaacg attcgtttgg tccgttcttt gttaactgaa aagcaaatcg aagaagaatg
    10981 ggcaaaatta cggcaaaatg ccaagaaaca aaaagaactt cttttgtgtt tatctgaact
    11041 gagccaagaa gaaccaattg cttatttcaa aaataaagga atcagtactg ctgtcttgaa
    11101 tcaaggaaaa gaaaagggct ggctggagtt tatagaatca gaacgttatc gcgaccctta
    11161 taaagaccga gtatttgacc aaacgacggc attggaactg aatgcagaac aaaaaaatgc
    11221 tgtcgaacaa atcatcacag caggtcagca gcaaaaagat caggtctttt tgctggaggg
    11281 aatcactggc agcgggaaaa cagaagtata tcttcaggca atcgctgatg ttttgtcaga
    11341 aaataaaacg gctattatgt tagtgcctga aatcgcattg actccgcaaa tggtcgagcg
    11401 attcaaagga cgcttcggag aatcagttgc ggtcttacat agcggacttt cccaaggtga
    11461 aaaatatgat gaatggcgca agattgagcg ggaagaagcg caagtagtag taggcgcacg
    11521 atctgctatt tttgccccgc tgaaaaatat cgggctgatc attatcgatg aagaacatga
    11581 atcaagttac aaacaagatg agacaccgcg ttatcatgcg agagatctag caatttggcg
    11641 cagtaaatat catcattgcc caatagttct aggaagcgcg acgccctctt tagaatcaag
    11701 agcacgagct caaaaaggcg tttatcagtt attgcagtta aaccaccgag ccaaagcagc
    11761 tgcacagctt ccagctattg agatcgtaga tatgcgtgaa gagtttcaga atcatcggac
    11821 ctctactttt tctgctaatt tgcaggaaaa aatacaaaat cgattagaca aaaaagaaca
    11881 aactgtccta ctgctcaatc gtcgaggata ttcgtctttt gtgatgtgta gggactgtgg
    11941 atttgttctt ccatgtccta attgcgatat ctctcttaca ctgcatatgg atacacgttc
    12001 aatgcgctgc cattattgcg gacatgaaga acctattccc aatcgctgtc ctaattgtgg
    12061 cggcaataaa atccgctact atggaacagg tactcaaaaa gtagaagagg aactccgaga
    12121 actttatccg caagctagga ttttgcgtat ggatgtagat accactcgaa gaaaaggggc
    12181 acacgaacag attttacaaa aattcggtgc aaaagaagca gatatattat tagggacaca
    12241 aatgattgct aaaggtttgg actttccaga agtgacatta gttggggtgt tgaatgctga
    12301 tacctccctg aatttaccgg attttcgttc tagtgagcat acattccaat tgcttaccca
    12361 agtttcagga cgagcagggc gagcggaaaa agcaggcgaa gtcgtgatcc aaacattcaa
    12421 tcctcaacat tacgccatag aattggctaa aaaacaaaat tatgagcagt tttatcagca
    12481 ggaaatgcat gtacggcatc gtggaggcta tcctccttat tactttactg tgaaaataac
    12541 cgccagccat cctgaagaac aagtagcggc aaagaaaatc tttcagattg ccaatcagct
    12601 aaaagaagtg ttaagccctc aaagcttgct attagggcca acgcccagca tgattttgag
    12661 agtgaaaaac cgatattatt atcaactaat catcaaatat aaacatgaac cgaatcttcc
    12721 tcatgttttg gatgaaattt tgaatggaag ccagaaagaa cagcgacaag gtctttttgt
    12781 ggcaattgac aacgaaccat taaactttat ttaagaaagg gccaaattat gcgctatcct
    12841 atattgatcc accctaatga taaattaaaa agaactgctc agccaatcga tgtaatcacg
    12901 gacgagacaa tcgctttatt agacaattta tatgagacaa tgattgcgaa tgacggaata
    12961 gggatcgctg ctcctcaagt aggacaaaac aagagaattg ctgtgatcga agtagatgaa
    13021 ggcgagaaat ttgaactgat taacccagaa atcatcgaag caaaaggtga aagccttgac
    13081 gtagaaggtt gtctcagtat tccgcatgtc tatggaacgg tcaagcgagc agatgaagtg
    13141 accgtgcgtt attatgatcg agacggagaa gagatagaag tgacggcttt tggctattta
    13201 gcccgtgcct tccagcatga aatcgatcac ttagacggta tcttgttcat tgagaaaatg
    13261 atacagcaga tcccggaaga agaacttgaa gaatatatgg aggaacatct tgatgactaa
    13321 gctagtattt atgggcacac ctgctttttc tgtgccgatt cttgaagggc tgatagaatc
    13381 aggttacgag atccaggcag tggtaacaca gcccgaccgt ccagttgggc ggaaacgagt
    13441 aattacgccg acaccggtaa aagaagcggc tctgaagcac ggtatccgtg tgctgcagcc
    13501 agagaaaatc tccggttcgc cagaaatgga agaaatcatt gaattggtgc ctgatgtgat
    13561 cgtcactgcg gcatttggcc aattcttgcc tgaaaagtta ttgcaagtac caaaattagg
    13621 ggcaatcaat gtccatgctt ctttacttcc taaatatcgg ggaggtgcgc ctgttcatta
    13681 tgccatcatg aatggggaga aagaaactgg tgtaacgatc atggaaatga tcaaaaaaat
    13741 ggatgcaggc ggaatctatg cgcaagaaag cataccaatc actaaacaag atgatgtagg
    13801 gacgatgttt gaaaagctta gccttttagg aagaaagcta ttgctagaaa cattgcccaa
    13861 tattttggat ggacaaaaac cagtacctca agacgagtca gaagcaactt tttcgccaaa
    13921 catcacacgg gaacaagaag caatcgattg gcataaaact gcagaagaaa ttgacaacca
    13981 agtacgcgga atgcgtcctt ggccaattgc attcacgacc tatgagggca tacgctggaa
    14041 attattatcg gttgaagtat tggcagaaac tacagacaaa gcacctggta cgatcatccg
    14101 gaaagacaaa aaaaatctat ggattgcttg cggtaaacag accgtcttag ccatcaatga
    14161 attacagcca gctggtaaag gaaaacaggc cgtacatgat ttccttaacg gaagcggtca
    14221 gcaagttcag attggtcagc aggtgaaata atgaaaaaaa tccctaaaaa aatgaaaaaa
    14281 tcagtacgat atatggcatt actgacatta gagcgagtga acaagggtgg cgcctactcg
    14341 aatcttttgt tgaatgagat gatcaaacaa agcgagctaa atcaaaaaga tgtgggttta
    14401 tttacagaat tgatctatgg tacgattagc catcagcgac tattggaatt ttacgttgca
    14461 ccattgatcg ctaaagcaaa aaaagtggat gactgggtca agacgcttct ttatctttct
    14521 gtataccaat tagaattttt agataaagtc cctgcacatg ctattttaaa cgaggcggtc
    14581 gaaatcgcaa aagtaaaagg aaatcctggg acagggaaat ttgtaaatgg tgtcttgagg
    14641 aattatcagc gtcaaggagc accagatctg caaggcattg ctgatcctat cgaaagactt
    14701 tctgtcaaaa tcagcctgcc tctatggatg acgaaacgac tggtggatca aatcggatac
    14761 gaagaaactg aaaagctcgg tttatcgcta tatcagccta gccatgctag tgcccgagta
    14821 gatacacgcc gactttccag agatcaggcc atcactgttt tgcaagaaga agagatcaca
    14881 gctgaaaaaa gccaagtcag tccatacggt atagtagcga aaaaagggca tttagcttct
    14941 agctcattgt tccacgatgg cgtcatgacg atccaagatg aaagttcgat gcttgtagcg
    15001 ccgtctatgc aaatcgagcc tgaacatcat gtcttagatg cctgtgcagc acccggaggt
    15061 aagacgacgc atattgcttc ttttttagat ccgtcatgcg gtggagaagt ggtcgcttta
    15121 gatgtacacg aacacaaagt caaactcatt gaagataatg cagcacgtct aggtgtctct
    15181 tctgctgtct acgctcaaaa gatggatgct agagaagtca gtaaacagtt cgaagcagag
    15241 cagtttgatc ggattttagt agacgcgcct tgttcaggtc ttgggctgct tcggcgaaaa
    15301 ccagatattc gttataaaaa acagccggat gaattagcga atttaccaaa aatccaattg
    15361 gaaattttag aaagctgcgc accaacttta aagtcttccg gtattctgac ttatagcact
    15421 tgtacgatct taaaagaaga aaaccaagac gttgtttctg ctttcttgga aagacaccct
    15481 gattttgaga ggattgatgt tttgaccaat cctatgctac aatcatcaat cgaagagaaa
    15541 atgttgactt tgtatccgca tcagttttac acagatggat ttttcatttg ttgtttgcgc
    15601 aaaaagtaat gaggtgaaaa aatgcagatt gaatatcaat cagatgtagg aagacgacgt
    15661 aacacaaatc aagactatgc cagtgtattt accagccgtt caggtctcaa gcttgctgta
    15721 ttagcagacg ggatgggtgg tcatcgtgcc ggagacattg ccagtcagat ggcggtaacg
    15781 aatctaggcg aagcttggga aaaacaagaa gctgttgacg atgaaaaaat cgcccaatgg
    15841 ttcattcaat cgattcaaga agaaaatatg ctgatttatc agcgtggaca agaacagccg
    15901 gaatataacg gtatgggaac aacaatcgta gcggccgctc tgtcagatga ctggttcacg
    15961 attgccaatg ttggggatag cagagcttat ttgattcgtg aagataaaat catgcaattg
    16021 acggaggatc attctttagt caatgaacta gtcaaatcag gagaaatcag cgaagaaatg
    16081 gcagtgaacc atccaaggaa aaatatttta acacgttctg tcggcatgcc tggaacagtc
    16141 gaagtagatg tttctactca tctttggcaa ctaaaagatc gactgctgtt atgttcagat
    16201 ggcttgacga acatgctgtc tgaagaagaa ataggaacga tcatcaatca agaaggccca
    16261 ttgtctcata aagtaactga gctgattgat cgtgccaatg aagcaggcgg agcggataat
    16321 atcaccgttc ttcttatcga atataaggag gacagtgtat gatcgagttg ggcaaaaaat
    16381 taaatggccg ttatcaaatc actggaaata tcggtagcgg tggtatggca aatgtatttt
    16441 tggcgcacga tttaatcttg gatcgtgatg tagcagttaa agtcttacga tttgactttc
    16501 aaaatgacca gactgcgatt cgacgatttc agagagaagc tttagcagca acagaactcg
    16561 ttcatccaaa cattgttagt gtttacgatg ttggtgaaga agacaacatg cagtatctgg
    16621 tgatggaata cgtcaaagga atggacttga aacgatatat ccaaacacat tatcctgttc
    16681 cttatgaaac agcagtaaat attatgcagc agatcctttc tgctatttct ttagctcata
    16741 accatcaaat catccaccga gatttgaaac cccaaaacgt actgatcgat aatgaaggtg
    16801 ttgtaaaaat cacggatttc gggattgcta tcgctttatc tgaaacatcg atcacgcaga
    16861 cgaatacgat gctaggatct gttcattatc tgtcccctga gcaggccaga ggaagtatgg
    16921 caacgaagca gtcagatatt tatgctttag ggatcatcct ttatgagatg ttgacgggta
    16981 gcgtaccgtt tgatggtgaa tctgctgtaa caattgcttt aaaacatttt caagatgatc
    17041 ttccatctat aaaagcactt gatccaaatg tgccacaagc actggaaaac gtgattttac
    17101 gggcaacggc taaagaacct gcagaccgtt ataagtcagc cgaggaaatg tctgatgatc
    17161 tttctacagt attatcacca gctcgtgcaa acgaagaaaa atggcagcct catgtgatgg
    17221 ataatgaaac aaaagttatc acacccctta cggaagatgc accaatgcct gattcattca
    17281 aatctatgcc tctgccaaaa gacaagccag aaaacgaaac cttaccagag gaggaagaag
    17341 ttctgcctga tcctaaaaag aaaggacaga aaaaatggtg gctgatccta ttagctattc
    17401 ttgctgtttt aggaatcgga acagctgtat tctttgcttc tggcgggcga ggagaagttg
    17461 ttgttcctga tgtttctgat ctttctgaag cttctgctag agaaacactc gcaaaagcga
    17521 aattagaagt agcaacaaaa acagaagaaa ttgcagatga caaaatcgaa gaaggtaatg
    17581 tagtgaagac agacccggca gcgggaacga cagtcaagca aaaacgcgaa gtcactctgt
    17641 atattagtag cggcacgaaa aaaatcaaat tagatgatta cagtggtaaa agttatgaag
    17701 atgccgaagc aagtttgaaa gaattagggt tttctgctga cttgattcaa aaagaagagg
    17761 aattcagttc agaggtagaa agcggaatga tcatttcgca atctccatca gaagatacag
    17821 aagtcgatcc taaaacagac acgattactt tcacagtcag caaaggacca gaaacagtga
    17881 ctttgagtag tcatgtagga gaaaactacg aaactgttcg taattggctt gttacacaag
    17941 gattcaatat caataatatt agtgctactt atgactatag cgacactgta ccatcaggtt
    18001 cgatcatcag tcaggatcca gcctcgggtg aagttgtagc agaagaaact tacatcaatt
    18061 ttgtcgtaag ccaaggaaca gagcctaaat tgaatgatat ctccggctat acaaaaagcg
    18121 aagcacaaag ttatttagct agtgtcggtg cagaatatat cgggcatgag acttccgaat
    18181 attcaaacac ggtggataaa gataaagtga ttcgtacagt accaggggca ggaacgacaa
    18241 ttaccaaagg aatggttgtg aacgtaattt actctaaagg accagatcca gcgtcttcta
    18301 gttctgaaaa agactcttca gacagttcta gcagctcaag tagctccagt agcacaagca
    18361 actcttcaaa agaagaatct tccagctcca caagttcatc agctagcacc tcagaaagtt
    18421 caaaataagt gctgaataaa atatatcaga agggaatgcg acattgattt gttgcattct
    18481 cttttttgtg tttctatctc ttttgatcaa ttttcaagcc atatactttg agaagtttgc
    18541 atggaataac acctactttc ctcttcgtat ggtaaaataa acaaaaacgg aggtggcact
    18601 tatcaaagga caaatcagaa aagcaatcag cggtttttac tatgtatacg caaacggaga
    18661 gacctatcaa acaagaggaa gaggaaattt ccggaatcgg aaaatcacgc cgcttgtcgg
    18721 agatcaggtg atctttgaga gtgaaaatca aacagacggc tatcttctgg aaatcttgcc
    18781 gagaaaaaat caattagttc gcccaccagt cacaaatgtt gatcttggaa tcattgtcac
    18841 aagtatcgta gagcctgcat tttcttataa tctacttgac cgctttctag ttaccttgga
    18901 atacgagtcg atcgaaccga ttattttttt gacaaagacc gacttgtcag acgacccaga
    18961 aaaaatcgaa gagatcaagc aaacctatac taaaataggc tatccggtga ttgtacccaa
    19021 tcatacgggt gatacgacag aactgatccg ttacttccca gaacggctat cggtctttat
    19081 gggtcagtct ggagcaggaa aatcgacgct actgaatcaa atatcaccgg aactgaattt
    19141 agcaacagga gagatttctg atgcacttgg aagaggacgg catacaacac gtcatgtgga
    19201 actgattcct atctatggcg ggttagtagc agatacacca ggatttagct cgattgactt
    19261 tttgacaatt gaagcaggag aactgccgag acaatttcct gaatttgtag aagcttctgt
    19321 ccattgcaaa tttagagagt gcatgcatgc taaagaacct ggctgcgaag taaaacatag
    19381 agtagaaaca ggtggtatcg cacagacacg ttacgacaat tatttgcaat tcttacaaga
    19441 aatcgaaaaa agaaaaccaa tgtatgcaaa aaaagaaggt aaacgaaaat aggaggaaac
    19501 aagaatgaag atagcaccat ccattttgag tgcagatttt gccaatttac aaagagacat
    19561 tgagttagtg gaaaaaggag gcgcagatta catccacgta gatgtaatgg atggccaatt
    19621 tgtcccaaat atcacattag ggccaaatat tgttcaggca atccgtcctg ttactaaatt
    19681 accgttagac gtccatctga tgatcgttga tccagaaaac tacattccag catttgcaaa
    19741 agctggagca gatatcatta ctgtccacgt cgaagcaact cctcatattc atcgggcact
    19801 tcaaatgaca aaaagcttag gagttaaagc tggagtagtt atcaatccag gaacaccagt
    19861 ctccatgatc aaacatgttt tgcctttggc tgatcaagta ctagtcatga ctgtcaatcc
    19921 tgggttcggc ggacaaagct ttattgaaga aacagtagaa aaaattacgg aactcgctga
    19981 tctacgtaga gagaacaatt ggacttatgc aatcgaagta gatggcggaa tcgttccaga
    20041 aacagcaaaa atctgtaaag atgcaggggc ggatgtcttc gttgcaggat catatatcta
    20101 tggagccgaa aatccagtcg gacaaatcga acgattaaaa gaggcattag actaaatgaa
    20161 aattttatta gctgctggag gtaatccgca aaactggccg cagtctctgc tgtccaaata
    20221 cgatgcctat gtcgggatag accgtggtgc gctgtacctg cttcaagcag gctacccagt
    20281 agatctggca gtgggtgatt ttgattcatt gtcgaacgaa gagcagaagg aagtatttgc
    20341 aaaagccaaa gagtataccc gttcccctgc agaaaaagac gatacagata cacagttagg
    20401 tatcttaaaa acatttgaac gttacccgca agcggaagtc actttgattg gagctacggg
    20461 cggaagattg gatcatcttt tagccaatct ctggttaggt atggaacctc gcttcaaacc
    20521 ctttgcccat cagcttatta tcagagattg tcaaaattat ttgtcttatt atttgcctgg
    20581 agagcatata gtaagacaga taccagatat gaagtattta gcttattgct gcttgactcc
    20641 cgtctcgaat cttacattaa aaggcagcaa gtatgtattg gaaaataaag aagtagaata
    20701 tccgacatct tatgcaagta acgaatttct ttcagatacc gcagaatttt ctttttcaga
    20761 tggtatgatt gcagttattc aaagcaaaga ttgatccata aacaaataaa aaaatttgtc
    20821 tttcaggcta aaaatgacag aatctattat ttgctaaaaa aaagcttaag aggagcaact
    20881 taccttttgt ttcacggcat ttagtgataa aattgaacac aagagtctaa ttaattcgag
    20941 gtgtctttgt gaagaagttc aatcctaata aaaatattat tattaccttg gttattgtta
    21001 tcatcgtggt gacgatacta agtgtgaccg ctgcgcagcg agcgaatgaa ggaaagccca
    21061 atttcttcca atccatcgtt aatgacagtg ttgcaatggt tgataaaaca atctctgccc
    21121 cagtacggtg ggtacgaaat ggagtagatt ctgtccataa tcttttttca acttatgccg
    21181 aaaatgagcg gctaaaagaa aaaatcgata gctatgatga cgtttcacag caaaacaaga
    21241 attacgagag agaaatcgaa gcgttgaaaa aagaacttga gttgaatcag acgctgacta
    21301 gttacgaaaa agtaacggca aatgtcatta ctcgttcgcc tgacagttgg caggacatgc
    21361 tgatcgtaga caagggttct aaagacggta tcgaagcaaa tatggcagtc atgtcgcaaa
    21421 aaggattgat tggccgagtg atcgaggtca atacggcttc gtctaaaatc gaattactgt
    21481 catcctctaa tgaaagctcc aatcattttc cagtacgggt atcttcggct aatggcgaag
    21541 cgtttggttt gcttaaaaac tatgatgaaa agctccatgc cttagtggtg acccaattaa
    21601 ctggtgatac ggatatcaaa gaaggggatg ttgtccagac atccggtctt ggagggaatt
    21661 ctccagctaa cttgccgatc ggtacggtta ttaaaacgaa accagatagt tatgggctgg
    21721 atcgggaagt ttatgtgaaa ccttatgcag aaatgtatga cgtgtcagtt gtgacgattg
    21781 tccaacggct agcggaggat tagtccatgt tgaagaaaga aacaatgaag tactatctgc
    21841 caatcgtttt gttctttttg atgttgatag atggtcattt aacaagaatg ctaggggagt
    21901 ggtcgaaagg cacctatatg tcaaatgccc actttctgat attggcatta ttatgttgca
    21961 gtatggcgtt tgaaaaacgt tatttactga ttaccacgat tgttctcggg gctatctatg
    22021 acgcttacta tattggcgtt atcggtatct atgcagtagc tctcccttta attgtatggt
    22081 tgatgtatgt aatgaaagac gttatccatg tcaacatctt tactgaattt ttcagtatga
    22141 tcatctttgt cacgggttat gaattgttta cgatggtggt ccagttgatt tttaaattag
    22201 cagtagtaaa taacacgtat tttattacaa ggtttttagg acctacac
//
LOCUS       DAZKCY010000034        22219 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000034 DAZKCY010000000
VERSION     DAZKCY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.034"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..515
                     /locus_tag="ACG8XC_001212"
     CDS             171..515
                     /locus_tag="ACG8XC_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9190924.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="ACG8XC_001213"
     CDS             516..935
                     /locus_tag="ACG8XC_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFP9190925.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1027..1377)
                     /locus_tag="ACG8XC_001214"
     CDS             complement(1027..1377)
                     /locus_tag="ACG8XC_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190926.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="ACG8XC_001215"
     CDS             complement(1343..2674)
                     /locus_tag="ACG8XC_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFP9190927.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="ACG8XC_001216"
     CDS             2930..3496
                     /locus_tag="ACG8XC_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9190928.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="ACG8XC_001217"
     tRNA            3641..3728
                     /locus_tag="ACG8XC_001217"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /gene="bsh"
                     /locus_tag="ACG8XC_001218"
     CDS             4062..5036
                     /gene="bsh"
                     /locus_tag="ACG8XC_001218"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFP9190929.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5361..7256
                     /gene="asnB"
                     /locus_tag="ACG8XC_001219"
     CDS             5361..7256
                     /gene="asnB"
                     /locus_tag="ACG8XC_001219"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFP9190930.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7451..7897)
                     /locus_tag="ACG8XC_001220"
     CDS             complement(7451..7897)
                     /locus_tag="ACG8XC_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFP9190931.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8125..8274
                     /gene="rpmG"
                     /locus_tag="ACG8XC_001221"
     CDS             8125..8274
                     /gene="rpmG"
                     /locus_tag="ACG8XC_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9190932.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8362..8880
                     /locus_tag="ACG8XC_001222"
     CDS             8362..8880
                     /locus_tag="ACG8XC_001222"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP9190933.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8957..9913)
                     /locus_tag="ACG8XC_001223"
     CDS             complement(8957..9913)
                     /locus_tag="ACG8XC_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9190934.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10079..10885
                     /locus_tag="ACG8XC_001224"
     CDS             10079..10885
                     /locus_tag="ACG8XC_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190935.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10882..11871
                     /locus_tag="ACG8XC_001225"
     CDS             10882..11871
                     /locus_tag="ACG8XC_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9190936.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11868..12872
                     /locus_tag="ACG8XC_001226"
     CDS             11868..12872
                     /locus_tag="ACG8XC_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9190937.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13004..13546
                     /locus_tag="ACG8XC_001227"
     CDS             13004..13546
                     /locus_tag="ACG8XC_001227"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP9190938.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13566..14264
                     /locus_tag="ACG8XC_001228"
     CDS             13566..14264
                     /locus_tag="ACG8XC_001228"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP9190939.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14285..14497
                     /locus_tag="ACG8XC_001229"
     CDS             14285..14497
                     /locus_tag="ACG8XC_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFP9190940.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14497..15459
                     /locus_tag="ACG8XC_001230"
     CDS             14497..15459
                     /locus_tag="ACG8XC_001230"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFP9190941.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15477..15872
                     /locus_tag="ACG8XC_001231"
     CDS             15477..15872
                     /locus_tag="ACG8XC_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9190942.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16035..16400
                     /locus_tag="ACG8XC_001232"
     CDS             16035..16400
                     /locus_tag="ACG8XC_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP9190943.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16397..18207
                     /locus_tag="ACG8XC_001233"
                     /pseudo
     CDS             16397..18207
                     /locus_tag="ACG8XC_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
     gene            complement(18269..18436)
                     /locus_tag="ACG8XC_001234"
     CDS             complement(18269..18436)
                     /locus_tag="ACG8XC_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFP9190944.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="ACG8XC_001235"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="ACG8XC_001235"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP9190945.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="ACG8XC_001236"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="ACG8XC_001236"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFP9190946.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="ACG8XC_001237"
     CDS             21193..22173
                     /locus_tag="ACG8XC_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9190947.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         7208 a         3805 c         4620 g         6586 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggcaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gacgggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agagagaaaa
     5281 tattcgtccg ctatttattc tatgataaga tgcatatgaa tgaaataaac agtaatttta
     5341 tattaggatg tgaaagttaa atgtgcggta ttgtaggttt tgttaacgac aaggacaaca
     5401 aaaaaacaat tattaataca atgatggatc gaatcgttca ccgtggacct aatagttcag
     5461 gagaatatat cgataaacat gttgctttag gattcagaag attaagtatt attgacttag
     5521 agggcggtac tcagcctatt tacaatgaag atcggacaaa gatcattatt tttaatggcg
     5581 aaatctataa ttaccagcct ttgagagaag aattgattgc agctggccac gtttttcaaa
     5641 cacatgcaga tacagaagtc cttttgcatg gttacgaaga atggggaaca gaattattgc
     5701 aaaagatccg cggaatgttt gcttttgcta tttgggataa tgaaaagaat gagttattcg
     5761 gtgcaagaga ccactttggg atcaaaccat attattatgc agaaatgaac ggaacattta
     5821 tgtttggttc agaaatcaaa agttttctgc ctcatcctga ttttaacaaa gaattgaaca
     5881 aagaagcgtt gaaaccttat atgactttcc aatattctcc attaaacggc gaaacattct
     5941 tcaaagatgt ctatcgtttg cctgaaggcc attattatac gtataaagat ggaaaaatag
     6001 acattcagca atattgggat gcagattttg aaacaaaaga aacgcattcc cgacaagaat
     6061 ggatcgaaaa aatcgacgaa accgttcaag cttctattga agcacatacg gtcagtgatg
     6121 tagaagttgg ttctttctta tccagcgggg ttgattccag ttatgtaacc tctgtattga
     6181 aaccagatca ttctttctca atcgggtttg atgataaaac ctataatgaa gcgattgaag
     6241 ccagaaaact aaccgaacta ttggacttag ataatacagc agcagtgatc gacggagaca
     6301 tgtcgttcaa agcattccct ttgatccagt atcacttaga tgaacctgat tcaaatcctt
     6361 cttgcgtacc tttgtacttt ttagcaaatc ttgcttcaca aagtgtgcgg gtagtccaat
     6421 ctggagaagg ggcggatgaa ctgtttgcgg gataccaaac gtatggattc catacgaatt
     6481 caaaattcat ccgtgtgatt gctcaaggat tgaaaaaatt accaaaagga acacgctata
     6541 atttaggacg taaaatcgga aaaatgaaaa atttccatgg acggatccat ttatatgaat
     6601 cacttgctcc tgcaaaagaa tattttatcg gtcatgctcg agtgttcgaa gaatcagaag
     6661 catcagaagt gttaacacca aaatatcaaa cagcaccatc tgtggatgaa atcatgactg
     6721 ttcattacga gaaaacagaa ggaatcaaag atgaagtcaa taagatgcaa tatgtcgatc
     6781 ttcatcaatg gatgccaaaa gatatcttgt taaaggcaga taaactttct atggcaagtt
     6841 ctttggaagt ccgagtacct ttattggata tcgaagtcat gaaattggct caacaaatac
     6901 caagtaagtt tttattgaat caaaacaaca caaaagatat tttcagacaa gctgcgaaca
     6961 aacatcttcc ggaagaatgg tcgaatcgtg tgaaattagg attccctgtg ccaatcaaag
     7021 cttggttgaa agaagaacac ggctacgaac aagtcaaagc attattcgaa gcagattttg
     7081 caaaagaatt ttttgatcaa gaaaaaatca tgaagttgct agatgaccat cacgaaggac
     7141 gtaaagaaga gcaaagaaaa atctggacaa tcttcagttt cttgacatgg tataaagtct
     7201 actttgtaga tgaaacgatt ccgcaagcag aagcaatcga ctatgtgact gtttgatgaa
     7261 aatcaaatat caataaaaaa gaggttttct cctaccgttt aacactgagt gataagaaac
     7321 aatttcaaaa aacagctctt cccgttttat agatattatg acttatggtt cggagttggc
     7381 agaggaacac cattgtttcg aacataaggg ggttgtgaca cgattttcgt cacaacccct
     7441 ttacatatgt tcaataatcg atttcttttt cttttgtccc atctttttta taaatgaaca
     7501 aagttgattc tttgttttgt gctacttgct ttcctttttc gattgcttct ttttttgtat
     7561 caaaatggtt gcttgccttt tttgcccctt tagatttcac gatccattgt tcttcctcat
     7621 attctacgat cacatctgaa tcaagcagtt ttccagcacg aggattcgta tcgtgttcat
     7681 ccgtcttgct tggatttttt tctttttcga aagttcgttt ttctgattca ctagcatctt
     7741 cataccattt ttttgcttga tcaatggcaa ttgggatcgc acggttatca gggtaccctt
     7801 catctaataa tgcatttgcg atgtcgattg ctttttttct tgtcaattta tctaagtttt
     7861 tcattgatgc aggaaagtcc ttcatgttcc atggcatatt agtatccccc tttattaaaa
     7921 aacataatct atataaacaa gtgtaagggt atttatacaa aaagaaaaag attttgtttg
     7981 agaagtaaaa ccgttgtatt tatcgtcaag tagttttact taacaaaatc gtgaaaaaca
     8041 ctagcaatct agaaaaaatc atggtaagat aatcaagtct gagagtcatc ttagaggatt
     8101 cttgtaaaaa tgggagggaa aaacatgcgc gtaaacatca ctttagagtg tacttcttgt
     8161 aaagaacgta actatctaac aagcaaaaac aaacgtaaca atcctgatcg tttggaaaaa
     8221 caaaaatatt gcccacgcga acgcaaagtt actttgcacc gtgaaacaaa ataatgtttt
     8281 taaagttctc aattccttgc tggtcaaaga attgagactt tttttatctc aaaacatcta
     8341 aaatcataag gagcggataa tatgtttatt tccatgtggg cacaagataa aaatggtctg
     8401 atcggcaaaa atggtttgtt accatggcga ttgccaaatg atatgcgttt ctttcgcgaa
     8461 cacacaatgg atcgaatttt agtgatggga agaaagacct atgaaggaat gggggatctt
     8521 tcacttcctt atcgccatat catcgtgttg acaacgcaac caaattttcg cacgaaagaa
     8581 aatgcggaag taatgcattc gatcgatgaa cttttgtcgt atagtcaaac gatctcagaa
     8641 gatatctatg tttcgggtgg tagtcggatt tttcaagaac ttcttcctta cactggtcgg
     8701 atctggcgaa cattgatcga tagcacgttt gaaggcgata cgtatattgg gaatattgat
     8761 ttttcagact tcgcccttat agaggagtat gaggggataa cggatcaaga aaatctatat
     8821 gcccatcgtt ttcaaaaatg ggaaagagta aaaaataggg ttgcttcaaa agaagaataa
     8881 aatctatgcg tctagtttgt taataagaaa aaaagcatct cacgactaat cgtgaagatg
     8941 ctttttttag ttaaaatcaa tttagttttt ctttgatatc attgaccatt tcctgactgg
     9001 caataggtcc gttatatagc caactagatt caggtccgaa ttgcagtaca tgattgtctg
     9061 tcactgcttt tagattcttc cagcttggtt cattgaacat ggcagcatct ttgtcactgt
     9121 tcacaaggat gatgtagtca gcatctaatt cagctagctt ttcagaggaa acagcagacc
     9181 agtcagaagt ggcttttttg gagacctctt ccactagatc aggaacacca aattccagat
     9241 cgtcgtatac gattcttcca cttgaccgat ttttgctgac cataaaggct gagttgttgg
     9301 tcacccacaa cactgcagct gtcttgttct cgattttatc tgctagttct tctcgagttt
     9361 tcttgatgga tttttgataa tcggaaatga ttttttctgc ctctttttct tttgccaaag
     9421 ctttgccgac atcttttagt tgttcttccc atgtgacatc tgttccgttt tttacaacat
     9481 aagttggcgc gattttgcta tattgttcgt atttcccgcc ttcgacagta gcggaagaag
     9541 aaatcaacag caaatcaggc tcaaagctta acactttctc ataaggaagg tcataagaaa
     9601 tcgttgggac atcctttaaa tcatcttgca gataatcttg aatggaacca ctgccaacag
     9661 tccactgggc gataggtttt tcccctagtg caatcagata gtcttctaag taactgccga
     9721 tgatccgttt gggcgcgttt ggaatctcta ctttatgtcc aagtgtatct gtgactgtat
     9781 gagtactatt ggatgaagtc gtcgaattgg cagttgtagc tgtttcttta tctgaagagg
     9841 gggagcaagc gctcagtact ccagccagca gcaatatgct agataatgtg aatacttttg
     9901 tttttaattt catttctttc ctcctaacat aattgagaat ctttctcatt ggttattatt
     9961 cctgatattt cgaacgctgt caaccgcttc atttggctat agatatgaaa gtagcgcttg
    10021 acgaaatgtt gaaacagcgt cataattgag aatgattttc agcgaaagga tgggtgaaat
    10081 gacgagatta agagcagctg acattgcagc cggctacgaa aacaaaaaaa tcattgaaaa
    10141 catgtctgtt acgattcctg aagggaaaat cactagtttg attgggccaa atggaagtgg
    10201 aaaatctact ttattgaaaa gttttacccg tatactacct gtgaaatcag gaaagatcat
    10261 tgtcaataac tctgatatct ctacatacag ttcaaaagaa cttgcaaaaa aaattgcatt
    10321 attagcacaa actagtgaac accctacagg tatgacagta gaagaagtag tatcttacgg
    10381 acgataccct tatcaaaaac tgttcagtgg catggacacg caagatcatg aagcaattga
    10441 gtgggcgttg gatgctacag gtctagctgg atttcgtatg cgggatatcg caacgctgtc
    10501 tggtggacaa acccagcgtg tctggatcgc tatggcgctt gctcaggaaa cagatatctt
    10561 gatcttggat gaaccgacaa catttttaga tccggcacac caattagaga tcctacagtt
    10621 attgactgaa atcaatcaaa tgaaacaaac aacgatcttg atgtcgattc atgacattaa
    10681 tcatgcttca cgtttttcag attatttatt agcaatgaaa aacggtaagt tgatcacaga
    10741 aggtacacct gaagaagtga tcacaaaaga gtgtatgaaa aaaatatttg agattgatgc
    10801 aacgattgtc acgcttccaa gaacaaataa accagttttt ctaacctatg atttgttgcc
    10861 aaaagagcag gaggatcaat tatgaggact atgaagaaaa atagactcct gcttttggtc
    10921 tgttttgttc ttttgattat tttttttgtt gctctttcaa atggcgcagc gactgtggga
    10981 aaagcagatt tttggcaggc gttgtttgat tttgatagat caaaacaaag tcaccaaatc
    11041 atacgaaata ttcgtttgcc tcgtgtggcg ggagcatttc ttgtagggag ttgttttgca
    11101 ttgagcggtg cattgatgca aggagtgaca agaaatcctt tagcagattc aggcttacta
    11161 ggaatcaacg cgggtgcttc gttggcaatg gcccttagtt ttgctttttg gcctaaagct
    11221 tctgattttt ctgtgtttct aatgtcattg gcaggttctt ttgctgtaac tttgctcgta
    11281 tttggttttt tgcagttttc gaaaatgggc atgaatcctt tacagctgat tttagctggc
    11341 gtagctatta gttctttttt tactgctatc agccaagcac ttacccaatt ctttaatctg
    11401 cagcaggatc ttgctttttg gtttgtaggc ggagctgcca atatcacatg gaaacaatta
    11461 gcagtattgt ttcctttata tatacttgct gttttggttt ctttttcatt aggaaaagca
    11521 atatctttga ttcatttgag tgaaacacat gcattagctc ttggaagtca tcctaaacga
    11581 acgcggttga ttgttttctg tctagttgca gtgttagcag ccattgccgt ttcgttagtc
    11641 ggacctgttt catttattgg gctgttggtt ccgccagttg ttcgcggaat ggttggaacg
    11701 gactatcgtt atgttctacc tgtctctttt ttagttggcg gtggattggt tatgttggcg
    11761 gattttacag cgaggatgat caatccccct tttgaaacac cttttggatt agtcattgca
    11821 ttgattggcg ttccgttttt actgatacaa gtaaggaggg aacaacggtg aaaaaaatat
    11881 attggacgtt ctatttcttg ctttttgtac ttgtcatcct gtctataggt agtttaatga
    11941 ttggtacgcc ttttatttac ctggatcagc tttttgccac gttcataggg aaagaatcat
    12001 cttctttggc gctgattgtt agagaatttc gagttcctcg attactgatc agtttattag
    12061 caggagcttg tcttggcgtt tctggttata tcttgcaagg agtaacaaga aatgaattgg
    12121 cagattctag tatattgggc attaatgccg gagcaggatt tttagtgatg ctttatttag
    12181 gtttcttttc gcactattcg ctgccgtttc tattgctagt tgtagccttt ctaggtggtg
    12241 ttttagctgc ttgctgtgtc tatcttgcgg cctatgatcg aaatggcttt ttgggtatga
    12301 atcgaatctt attgtccggt attgccgtta atgccggcct ttctgcatta acattgctat
    12361 gcacgatcca attatctaag gaaaattatg gatttgtgaa tgcatggtta gcgggatcaa
    12421 tctggggtgc gagctggcct tatgtttggg cattgcttcc atgggcggtt atcttaattc
    12481 cgctaggagg actttatgca cagaggattg tattgctatc atttggtcaa gaacgcgcgc
    12541 aaggattagg ccttaacgtg gctaaaagtc aaaaaatcct tttgttgtta gctgttgctt
    12601 tggcaagcgg tagtgtagcg gtgacaggta gtttatcttt tgtaggacta ctagctcctc
    12661 atgctgcgaa gctagtcgta cgtaaagaaa ataactggac ctttgttctg agcggtttgt
    12721 tcggttccat ttttctgctt agtgcagata tggttgctag agtgattcta ctaagtgggg
    12781 aagtacctac aggaattttg attgcccttt ttggtgcgcc atattttctg tatgcacttt
    12841 ttatcagaca aaaacacgcg ctgaacagtt gaaattcatt aaaaaaggaa ggccaatcat
    12901 ttctaggtct tctttttatt ctatttatgc atttatttga aagttcgatt gctatttttc
    12961 tttttttcct ataccatttt atgagagaag gaagggtgag tttttggacc aattgaaagt
    13021 aagattacgc cggaaaggaa tcgaaaagct gaaaaatctg gccaaaagtg gcaagaaaaa
    13081 ggaaaaagaa cagcgaatct ataaacagct attcgccagc gagtactgga agtcagcaaa
    13141 agtgataggg acgactttat cgaatgaatt tgaactggat acacagccgg ttatccaacg
    13201 agcagaaaaa gaacaaaaga aaatagttgt acctaaaact ttgccgaaac gtcagatggc
    13261 gttttatgaa atggatgaac agtccgttct tgtacgttcg agttttggtg ttcttgagcc
    13321 acaatcagag aagctctatt tgccggagat gatcgatctc ctcattgtac caggtattat
    13381 ctacaataga gccggttatc gaatcggttt tggcggtggt tattacgacc ggtatttaaa
    13441 taattttcat aaaaaaacat gtagccttgt ttttcgtgaa caattgaatg aacaatggca
    13501 accggagagc tttgatcaga agatccaaca attgtttatt gactaaaatg aaatgggagg
    13561 ttttcatgaa ttatcaacag caaataaaat tgcgtgcatg gttaagacga ccgttatgga
    13621 cctatgcttt tttagggata cagacagcgg tcttcctcat gatggaatta tttccgcgac
    13681 ttgagatccc ttattatgcc ggaatgtatg gtccttatct tgttcacttc aatgaatggt
    13741 ggcgtttagt gacaccaatc ttcatccatt ttggactgat gcatttcgta atgaactcac
    13801 tgatactgta ttttatgggg cagcaaatcg aggcaatcta tggccattgg cgttttttct
    13861 tgatttatat gttcagcggg ataatgggaa atacagcaag ctttgctttt aacgaagcga
    13921 atgttctgtc tggtggtgca agtacttcga tattcggttt gttcggtgcg ttgtttatct
    13981 tagggtttca ttttaaacat aacccagcga tccagcagct agtcagacat tttcttttat
    14041 ttattgtgtt gacatttgtt tttggtcttc tggatacctc tgtagatgtt tggggacatg
    14101 tcggtgggct aattggcgga ttagtgttag ggaatgtttt gggtctaccg aaacaacaga
    14161 caagctattc gatacaccaa aggattcttt ctacactagt ttttgtattt ctttttgtaa
    14221 tatgtatttt actgggttta aaaaaatatg gtttacttgt ataatatgaa ggaagtgctt
    14281 aaagatggag agcttatatg atgtacagca gctgttcaaa aaatttggca tctttatcta
    14341 tgtgggtgca cgcatctatg acattgaatt aatgactatc gaattgcgta atctgtatga
    14401 tagccgtttg attgatcatg atacatacat gacagcgtgg cgcatattaa aaagagagca
    14461 ccgaattgaa gaaagccgaa agaaaggaaa tttgtaatgg acaagaagat tattgggatt
    14521 gatttaggtg gaacaacagc aaaatttgca attttaacac cagaaggaga gattcagcaa
    14581 aaatggagca tcgataccaa tattttggat gatggtaagc atatcgttcc tgaaatcatt
    14641 gaatcaatca atcatcgttt gaatttatat aatatgaaag ccgaagactt tatcggaatc
    14701 ggcatgggta ccccaggaag cgtggacagc gaagctggaa cggttattgg tgcgtataac
    14761 ttaaactgga cagaagttca atttgtgaaa aaactgatcg aggcaggaac aggaatcaaa
    14821 ttcgctatcg ataatgacgc gaatgttgct gctttaggtg aacgatggaa aggtgctgga
    14881 gaaaacgatc cagacgttgt attcgttact ctaggaacag gtgttggcgg aggtatcgtt
    14941 gctggcggaa atttgattca cggcgtgagc ggagctggag gagaaatcgg acatatcact
    15001 gtggatcctg aagggttcga atgtacttgc ggtaaacgcg gatgtttaga aactgtatct
    15061 agcgctacag gtgtggtacg cctaggtcgt ttcttggcag aagaatacgc aggggattca
    15121 aaactgaaag caatgctaga caacggagaa gaagtaacaa gtaaagatat ctttgaaatg
    15181 gctcaagaag acgatccgtt tgcattgatg gttgttgatc gcgtatgctt ctacttaggt
    15241 ttagcttgcg gaaacttggg gaatacatta aatccgtcaa gtatcgtgtt aggtggagga
    15301 gtatcggcag caggagaatt tttacgtagc cgtgtagaga aatattttaa agaatttact
    15361 ttcccgcaag taagagaatc aacaaaaatc aaattagcag agcttggtaa tgaagctggt
    15421 gtcattggtg ctgcctcatt agccttgaaa tttgcttaaa aaaacggagg cattacatgg
    15481 ttttatgggt aattaatatc attttgctat tgattgtgct tgcaatcatc ctttgggaag
    15541 tctatctacg agtaatggcc aaacgttcag caacgacttt gacagaagaa gaatttcgag
    15601 aaggaatgag aaaagcacaa gtaatcgatg ttcgcgaaaa agatgtgttt gatgctgggc
    15661 atattttagg tgcaagaaac atcccttaca ctgttttgaa agattcatta ggatctattc
    15721 gaaaagacca gcctgtctat atttacgatc aaaagaaaag tttgagcatc cgtacagcaa
    15781 ataaattacg taaagcaggt tatcaagaga tttatttact taagggtgga tatgacggat
    15841 ggtctggaaa aatcaaaagt aaaaaagtat aaaacagatg attgtatgac agctttaaaa
    15901 accgaatcaa tcattagatt ttgaccaaga tcacatgaac ggcgcaatgg atagttgtgc
    15961 cgttcatttt ttttattttg tgctattatc tacatatata agctaagcga aaaggtagaa
    16021 agaaggaaac aactatgatc aaattaaaac gtgtttacct tgatgctgaa aaagaggatg
    16081 gctatagaat actcgtggat agattctggc cacgaggaat ttcaaaagaa aaagcgcagt
    16141 tagatctgtg gttgaaagaa attgcaccta gtaatgaagc acgcaaaact tttgaccatg
    16201 taccagaacg atttgcttca tttgcaaaga aataccagca agaattgatt ttcgaagaag
    16261 aaaagaagca agctattttt aaactcagtc aacttatgaa agaacatcca gtcattactt
    16321 ttgtttatgg tgcaaaaaat gaacatcaga accatgctgt tttattgaaa aaatttatcg
    16381 aaatggaggc atgcaaatga aaatagggat cattggtgcc ggacctagag gattaagtgt
    16441 agcagaaagg attctccata ataatcagga cagacattct cttgaacttt ttcttttcga
    16501 tcccaatggt cctggaggtc gagtttggcg gttgaatcag ccaccggagt tattaatgaa
    16561 ttctgtttct caacaagtga cgctttttac agatgaaaca ttgtccagtg gaggattggt
    16621 aacacaggga cccaacttgt ttcaatggag taaaggacct gctacagatt atatcgaaca
    16681 gacggtaacc aaaaataagc aactaattat agaagaaatc aatcgtctgg aaaaagatca
    16741 gcaatctact cgatgtttat acggactcta tcaacgctgg tttttctcaa agttgcaaga
    16801 agaatttcca gcttctattc agtttgttta ttctttagta aaaaaagtaa gcaaaagaaa
    16861 agatggttat cttttacaga cagatcagca aagctattct atcgatcagc ttgttttagc
    16921 aagcggtcac tgggaaaatg atgagacaga agaagaaaga gtgtgggtag actacgcaaa
    16981 gaaaaatgtt ttactgtatc agcttccttc taatcctgca gatgtagatc tttcgaagat
    17041 accgcctaaa gaaaatgtct ttttaagagg tctaggctta agtttttttg attatgtcgg
    17101 attgtttaca gtatcaagag gagggcgctt cgagaacgaa aagggaaaac tggtctatca
    17161 tccatcaggc aaggaaccga ttgtatacag tggatcaaga agggggctcc cttattaccc
    17221 tcgaggaaga aatcaaaagc aaggcggagc aatggcatgg ccacggttac taacaaaaga
    17281 aaacctggag caatggcatc agtcgggatt tttgactggc gaaacatttt ttgactatct
    17341 gaaaaaagac gttgagttgt tttattacaa gaaaatcatc gaagaatatc acttgccgat
    17401 tccaaagaaa aattttgagc atgattttct ctccctctct tcagaggagt gtctcggaaa
    17461 gcatccagca ctaatccctt atagatggga ttggtcgtat ttagaaactc ctcttgctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agctttttt
//
LOCUS       DAZKCY010000035        22042 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000035 DAZKCY010000000
VERSION     DAZKCY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.035"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..394
                     /locus_tag="ACG8XC_001238"
     CDS             167..394
                     /locus_tag="ACG8XC_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP9190948.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            410..1576
                     /gene="hemW"
                     /locus_tag="ACG8XC_001239"
     CDS             410..1576
                     /gene="hemW"
                     /locus_tag="ACG8XC_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP9190949.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1811..2854
                     /gene="hrcA"
                     /locus_tag="ACG8XC_001240"
     CDS             1811..2854
                     /gene="hrcA"
                     /locus_tag="ACG8XC_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFP9190950.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2948..3511
                     /gene="grpE"
                     /locus_tag="ACG8XC_001241"
     CDS             2948..3511
                     /gene="grpE"
                     /locus_tag="ACG8XC_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP9190951.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDRYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3562..5391
                     /gene="dnaK"
                     /locus_tag="ACG8XC_001242"
     CDS             3562..5391
                     /gene="dnaK"
                     /locus_tag="ACG8XC_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP9190952.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5542..6708
                     /gene="dnaJ"
                     /locus_tag="ACG8XC_001243"
     CDS             5542..6708
                     /gene="dnaJ"
                     /locus_tag="ACG8XC_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP9190953.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7076..7813)
                     /locus_tag="ACG8XC_001244"
     CDS             complement(7076..7813)
                     /locus_tag="ACG8XC_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9190954.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7815..9317)
                     /locus_tag="ACG8XC_001245"
     CDS             complement(7815..9317)
                     /locus_tag="ACG8XC_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFP9190955.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            9576..11570
                     /gene="uvrB"
                     /locus_tag="ACG8XC_001246"
     CDS             9576..11570
                     /gene="uvrB"
                     /locus_tag="ACG8XC_001246"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP9190956.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            11580..14396
                     /gene="uvrA"
                     /locus_tag="ACG8XC_001247"
     CDS             11580..14396
                     /gene="uvrA"
                     /locus_tag="ACG8XC_001247"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP9190957.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            14659..15924
                     /locus_tag="ACG8XC_001248"
     CDS             14659..15924
                     /locus_tag="ACG8XC_001248"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFP9190958.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            15928..16659
                     /locus_tag="ACG8XC_001249"
     CDS             15928..16659
                     /locus_tag="ACG8XC_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFP9190959.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            16794..17678
                     /gene="rapZ"
                     /locus_tag="ACG8XC_001250"
     CDS             16794..17678
                     /gene="rapZ"
                     /locus_tag="ACG8XC_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP9190960.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            17675..18673
                     /gene="yvcK"
                     /locus_tag="ACG8XC_001251"
     CDS             17675..18673
                     /gene="yvcK"
                     /locus_tag="ACG8XC_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP9190961.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            18707..19642
                     /gene="whiA"
                     /locus_tag="ACG8XC_001252"
     CDS             18707..19642
                     /gene="whiA"
                     /locus_tag="ACG8XC_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFP9190962.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            20210..20866
                     /locus_tag="ACG8XC_001253"
     CDS             20210..20866
                     /locus_tag="ACG8XC_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190963.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            21045..21610
                     /locus_tag="ACG8XC_001254"
                     /pseudo
     CDS             21045..21610
                     /locus_tag="ACG8XC_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7285 a         3733 c         4834 g         6190 t
ORIGIN      
        1 ataaaataag gacaaaattg tttaggaaga atcatgagtg taaaaaaatc atccaagaag
       61 tcgattcttg gattttttag tggatttgtt cgccaaatac gtttagtatg cgtatatgag
      121 aggttgtaag caaagcgttg tggtctgagc acttaaggag gaatctgtga aaatagcttt
      181 tcatattttt gcagattttg acttaatgcc gaaggtcagc tttgcgatta ccgtttatcg
      241 agaggctgta aaagcaccgt gtagctccga tgacaataag caagcatttt tagagctagg
      301 tttccttgat tctaaatatg cggcttattt caatgagttg gtgcttgaac gcgattttaa
      361 gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa agaatttcaa tgatagaaac
      421 aataaatccc gcagaaaaca aggtttctgc gtacattcat atcccatttt gcgagcatat
      481 ctgctattac tgtgacttta ataaagtttt tttagaaggg cagccagtag atgagtatgt
      541 ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt catcctacag cgaatttgga
      601 aacactttat gtaggaggcg gaacaccaac ttctttatct gctaaacaac ttgatcgact
      661 attatcaggt gctagagaga tcctgccttt tcaaaatggt aatgagttta ctgtagaagc
      721 gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg aaaaactatg gcgtcaatcg
      781 tttatccatg ggagtgcaga catttgacaa tcgcttattg aaaaaaattg gacgtaaaca
      841 tactgcagaa gatgtgtatc aaacaatgca atttttagaa gcagaaaatt ttaccaatgt
      901 aagcatcgat ttgatttatg cattaccggg acaaacacta gaaggatatc gggatacttt
      961 ggaaaaagct ttggctttgg atctgcctca ctattctttg tactcattga tcctcgaaaa
     1021 caaaacaatg ttcatgaact gggtacgaca aggaagactc gaactgcctg atcaggaaac
     1081 ggaaaccaga atgtttgaag agacgattga agcaatggaa aagcatggac gtcaccaata
     1141 tgaaataagt aactttgggc tctccggaca tgaatctcaa cataacctca tgtactggaa
     1201 caatgaccac tattttgggt ttggagctgg tgctagcggt tatcttggag atattcgtta
     1261 ccgcaacaaa ggaccgatcc aacactattt acgcccatta cgagcaaatg aacgcccggt
     1321 attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa gaagaaatgt tcttaggact
     1381 aagaaaaaaa acagggatta ctaaaaaaca tttctatgat cgctatcaaa aaacaattga
     1441 atcggtgtac ggtgatgtgc taagggaatt agagcaagaa gggttacttg ttaatgaaga
     1501 agaccgtgtt tatttgacac caagagggac ttttttgggg aacaatgtgt ttgagcgctt
     1561 tttatttgaa aaatagtttg aagaatattt gttttttatc gaaacttgaa aagaaaaagt
     1621 ttagaaagtt tgacaatgag gtattgaagt attgatataa ctggatttac atcaattatt
     1681 tagaattagc actcttgtgt ttagagtgct aaaaaatgga gtctttctct tgacaggaga
     1741 agggcttctt gctatattta taagtgaatt agcacttaga atatatgagt gctaatgaga
     1801 ggtgaggaag atgttaacaa agcgacaaaa cgacatttta cgtctgatca tccaacacta
     1861 tacgagtagt ggcgtgcccg ttggttccaa aacattaatg tctgagggag tcaaggccag
     1921 ctcagcaacg attcgcaatg atatgaaagc attagaagat gaaggattgc tgttgaagac
     1981 tcactcttct tctgggcgga ttccatctgc tttaggctat cgttattacg tggatcattt
     2041 gttaaaacct gctcgcgtag cagaagatga tttgctgctg attcgtcagt ctctaggcag
     2101 agaatttcat gaaatcaatg agattattaa gcaatctgct gagatattat ctgagctgac
     2161 aagctatacg actttttcat tgggacctga aataaaagac cgccgactga ctggctttcg
     2221 tatcgtaccc ttgaataatc gacagattat tgctatcgtc gtaacagata tggggaatgt
     2281 agaaagccaa gtgttcactc ttcccgaata tatgggaagt caggaactag aaaaaatggt
     2341 acagattgta aacgaccgtc tagtcggaga tcctctgatg acggtgtacc ataaacttcg
     2401 tacggagatt ccgatgattc ttcataaata tttccagacg acagaaggga tgtctcagtt
     2461 atttgacacc gttcttggtc atgtttttga agaaaaggtt tatgtcagtg gacaaatgaa
     2521 tctcttggat tatgagcccc gtcaagatct tgatcagttc aaatcgatgt tttcttttat
     2581 gaaagactca gacggactta cacaaatgat cactccgatg gatagcaata ttcatatcaa
     2641 gattggtgct gaattaggca atgatttatt gcagaatatg agtatgattc aagcaagtta
     2701 cgagatagat ggtcatggaa gaggtacaat agccttgtta gggcctacta gtatgcctta
     2761 ttctcggatg ttcggtctag tcgatgttta tcgaagagaa ctagcagcta aacttgctga
     2821 ttattaccgc tcgcttgact tgtcaaactc gtaatgtgaa taacgaagct atttgcagct
     2881 gatcgttctc tctttcttag taagagcaat caaacagctg actgaataat gaaaggaaga
     2941 ctgtatcgtg aaagaaaatc aagaagaact agataaagaa atgacagatg ctcagccaga
     3001 accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt tcagaagctg aagcggaaga
     3061 attcgaaaca gcgaaattaa aagctgagtt agaagaaatg gaagatagat acttgcgggc
     3121 tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat gagcgtgagc aattacaaaa
     3181 ataccgttca caagatttag ccaaaaaatt acttccatca attgacaacc tggaacgtgc
     3241 attagccaca gaagtgtcag atgatcaagg ggcaggtttg aaaaaaggcg tggaaatggt
     3301 cttggaaagt ttgcgtaacg cactggaaga agaaggaatt gaaaaaatcc ctgctaaagg
     3361 agaagccttt gatcctaatc ttcatcaagc agtacaaact gttccagcga ctgaagatac
     3421 accagccgat acgattgtag aagtactaca agaaggctac aaattacatg atcgcgtgtt
     3481 aagaccaact atggtcattg ttgcacaata attgtgcgga tataaaactt acactttcaa
     3541 ttaaagaagg agacgttaat tatgagtaaa attatcggta ttgacttagg tacgactaac
     3601 tctgcagtag cagtattgga aggaaacgaa gcaaaaatca tcgctaaccc agaaggaaac
     3661 cgtacaacac cttctgttgt atcattcaaa aatggagaaa tccaagtagg ggaagttgca
     3721 aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa tcaaacgcca tatcggtgaa
     3781 gcaggttaca aagtagatgt agaaggtaaa tcatatacac cacaagaaat ctcagcaatg
     3841 atccttcaat atatcaaagg attcgcagaa gactatttag gtgaaaaagt cgaaaaagca
     3901 gttatcactg tgccagctta ctttaatgat gcacaacgtc aagcaacaaa agatgctggt
     3961 aagatcgctg gtctagaagt agaacgtatc gtcaatgaac caactgcagc tgctcttgcc
     4021 tatggtttag ataaaacaga tcgcgacgaa aaaatcttag tattcgacct tggtggcggt
     4081 acttttgacg tatctatcct tgaactagga gacggtgtct ttgatgtatt gtcaacagct
     4141 ggcgacaacc atctaggtgg ggacgacttt gataacaaaa tcatcgacta catggtagca
     4201 gaattcaaaa aagaaaacag cattgacttg tctcaagaca aaatggcttt acaacgcttg
     4261 aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg tatcaagcac acaaatcagc
     4321 ttgccattta tcacagcagg agaatctgga ccattgcatt tggaaatgac attgacacgt
     4381 gcaaaatttg atgaattaac agctgactta gtagaacgta caaaagtacc agtacgtcaa
     4441 gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg aagttatttt agtcggtgga
     4501 tctactcgta tccctgcagt tgtagaagca gtacgtaaag aaacaggaaa agaaccaaac
     4561 aaatcagtta accctgatga agtagttgct atgggtgctg ctatccaagg tggcgtgatc
     4621 actggtgatg tgaaagatgt tgttttactt gacgttacac cattgtcatt aggtatcgaa
     4681 acaatgggtg gagtgttcac taaattgatt gaccgcaata caacgattcc aacaagtaaa
     4741 tcacaagtat tctcaactgc ggcagataat caaccagcag tagacatcca tgtattacaa
     4801 ggtgaacgcc caatggctgc tgacaacaaa actctaggaa gattccaatt gacagatatt
     4861 ccagcagctc ctcgtggtat tccacaaatc gaagtaacat tcgatattga taaaaacggg
     4921 attgtaaatg tatctgcgaa agatctaggt acacaaaaag aacaaaaaat cacgatcaaa
     4981 tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg tgaaagatgc agaagcaaac
     5041 gctgaagcag ataaagctcg taaagaggaa gttgacctac gtaatgatgt agacgcattg
     5101 ctattctctg tggacaaaac attgaaagaa ttagaaggca aagtggacga agaagaagtg
     5161 aaaaaagcag aaacagctcg tgacgaatta aaagccgctg ttgaagcaaa caacatcgag
     5221 gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc aaaacttgac tgttaaacta
     5281 tacgaacaag ctgcacaaca acaagctcaa gaaaatccag aagcagctca aggcggagca
     5341 gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg acgataaata atcagttcac
     5401 tgaatttcga gagaaggcca aagttgctac tttggctttt tcttttgaaa aatagtttga
     5461 agtatcggtt tttgtttcaa acaaaatatg atatgataac aagcgacgtg aaaatgatga
     5521 gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt attatgaagt cttagggctc
     5581 tcaaaaggag catcagaaga tgaaataaag aaagcttatc gtaagctttc gaaaaaatac
     5641 catcctgaca tcaataaaga acctgatgcg gaagaaaaat tcaaagaagt atcagaagca
     5701 tatgagattt taagtgaccc gcaaaaacgc gctgcatacg accaatacgg gcatgcaggc
     5761 acagatccaa attacggtgc tggtggagct ggtggcggat ttggtggatt cggcggcttc
     5821 tctggtggcg gcttcggtgg ttttgaagat atcttcgact ctttctttgg aggaggcggt
     5881 cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt tgcaatatac ggtcgacttg
     5941 tcttttgaag aagcgatttt tggtgtagaa aaagaaatca agtataatcg ggaagaaatc
     6001 tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac aaccaactac ttgtcataaa
     6061 tgtcatggtg caggtacgat caatgtagaa cgccaaacgc cacttggacg agtgatgagc
     6121 cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa tcaaagaacc ttgtccaacc
     6181 tgtcatggct caggacatga gaagaaagca cattcagtta aagtgaatgt accagctggt
     6241 gtagaagaag gacaacaaat gcgtttagct ggtcaaggag aagctggaga aaacggcgga
     6301 ccatttggtg atttgtatgt ggtttttcgt gtagaagaaa gtgatatctt tgatcgtgaa
     6361 ggttctgaaa tttactatga attgccttta agttttgtac aagcagcttt gggggatgaa
     6421 gtccaagtac caactgtcca tggagatgtg aaattgaaga tccctgctgg aacacagaca
     6481 ggtacgaact tccgtttacg tggaaaaggc gctccgaagt tacgtggtgg tgcaacaggc
     6541 gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc taaatgaaca acaaagagaa
     6601 gcattacgtg cattcgctga agctggcggc atcaaagttg aagaacagca agaagacggt
     6661 ttcttcgata agtttaaaga tgcttttggc gggaaaaagc ggaagtagtt tttctagtaa
     6721 aaatataaag tagacttgcc caaattttct atttctagta aaaacgctaa gtcattaata
     6781 attaaaaacc agacgaacaa tcatacgttc gcctggtttt tttgtagttg tcatgtttgt
     6841 accaacaaaa aagccagctt tagaataaga taatcatcat agtttctgaa ggtggtatcg
     6901 actatgtact tgatatcgtc aatgaaccga tggtactttc ttatgcgatg gaacaggtag
     6961 agcgattgct tgtttgtgca ggagaacaag cgatgcggct ggtacagtta agtgaaaaaa
     7021 caagaaacta aaaatgcccg gtcgtcatga agcgaccggg catttttggt ttttattaaa
     7081 tatttaatac tttattcaag aaatcttttg tccgttcatt ttgcggattt tcaaagattt
     7141 gttgaggcgt tccgtcttct aaaaagtttc cgtcgtcaat gaacatgaca cgattggcca
     7201 cttctttagc gaagcccatt tcgtgcgtaa cgataaccat cgtcatacct tgttctgcta
     7261 actttttcat gacgttcaag acatcaccga ccatttcggg gtcgagtgca gatgtaggct
     7321 catcaaaaag catgatgtca gggttcatcg ctagagcacg agcaatagcc acacgctgct
     7381 tttgtccacc ggaaagcatt tcaggataca tatctttttt atcttctaga ccaactgtct
     7441 gcagcaattt gattgctttt ttctctgctt cttctctaga ttctttcttc aggtccatcg
     7501 gtgctaaaat gatgttttcc aaaatagaaa gatgagggaa taggttaaag tgctggaata
     7561 ccattccgat atgttgtctc accaagttga tatctgtgct tttatccatc aagtgggcgc
     7621 cgtcaataat aatttcaccg tcagaaggtt cttctaaacg attcaagcaa cgcaaaaatg
     7681 tactttttcc tgaacctgaa gggccaatga tacagacgac atctccttcg ttgatggaca
     7741 cattgatgtc gtttaatact gtgttatcgc cgtatttttt gactaaatgc tcaactaaga
     7801 ttttttctgc catcttactt caccttcttt tctaaaactt ttgctaattt tgtcaatgct
     7861 gtgatcaaaa tcaagtacat gatcgcaatg atcaagtaaa catacgtact ttgtgttgta
     7921 cgtgcaacaa tgatcttacc agtttgcagc aattcgacta caccgattgc ggaaatgatc
     7981 gttgtgtctt ttaatgagat aacgaactga ttgacgaatg atgggatcat gatgcgtatc
     8041 gcttgcggca aaatgatttt ttgcattgtc cgattataag aaagacctaa acttctggaa
     8101 gcttccattt gtccgacagg aacggcgttg atcccgcctc gaacaatctc tgcgatataa
     8161 gcacttgcgt taagagtcaa ggtgataaca ccagcaagga agtcaggaat cgtaaatcca
     8221 acgataccag gcaacccaaa gaaaataaag aacgcaagaa ccatcattgg aattcctcgg
     8281 atgatatcga cataaatact agcaattgtg cgaagagcgc gtattggtgc tacactgaat
     8341 aatccaaaaa tcacaccgat tactatagcc aaagcaaagg agatcaaagt taggagaatt
     8401 gttctccaaa gtcctttcaa caatgcttga tagttgtttt ggataagtcc aagaaatgtt
     8461 gattcatcag cagtggaaga ctcactggaa gtttctgaac catccgctac atattcttta
     8521 ataatcttat cgtattcgcc tgttcttttt aattctttca atccttcatt gaacatatta
     8581 agaagctcag gattttgtcc ttttttaacg gcaaatgcat attttccgcc agattctttt
     8641 ttgatcggtg tttccaattc ttgtccttgt ttgatcgcat aaccgattac cggataatca
     8701 tccatcattg catcgattgc gccaactttt aacccgtcgt acaattgatc agcagcatca
     8761 aaatatttga tagaatagcc atattcagat tcatggtcgc ttaaaaattc agcactttcg
     8821 gtaccaactt ttgctccgac tgtttttcct ttcaaatctt cataagactt gatcgaagta
     8881 tctccttttt tgacagctaa ttggatcccg ctatcaaagt aagaatcaga aaaatcatag
     8941 cttttctttc gttcatctgt tacagtcatt ccggcaatca ttccgtctgc ttgtcctgat
     9001 tccaaagctt gcaaagcagc agagaaacca cggtagtcca ttcggatgtt aaatccttgc
     9061 agtttggcga tgttttccat caattttaca tcgatgcctt cgtactcgcc ctttgcgttt
     9121 tgaaattcaa atggagcaaa agcagagtca ctggcaatta cgtatacgtc ttttttgggc
     9181 tctatttttt gcattgtctc gcttgtgctt gactctgccg ttgtagtgtt gtcagcttgt
     9241 gcaacatgtc caaacgcaag tgtcacaagt cctgtcatta agaaaacaaa tgagtgaaat
     9301 aaatgctttc ttttcataat caacctccta aatttccaat attttattat tataggcgaa
     9361 aagtgctgaa tcagacaaga ctgtaagctc gaaaaaatga aaaaaatgcg agtttctaac
     9421 attttatgtt ataaaacgta acatgcataa taagataaaa aagtggcgaa agtatgttcg
     9481 gtgtggtata attttttagt aaggaatgcc tttgctttcc ttttcttttt attggtcgta
     9541 aaatgaaaag gaatgaatga aaaggaggac gaagaatgat tgagagagat acctcccgac
     9601 actttgagct tgtctcgaaa taccaaccgg ctggtgatca gcctgaagcg attaaccaat
     9661 tagtggatgg cgtagtcggc gggaaaaaag cacaaatctt attaggtgcg actggaacag
     9721 ggaaaacata tacgatttcc aatcttattg agaaagtgaa taaaccaaca ctaattattg
     9781 cccataacaa aacattggct ggtcagctgt acggggaatt caaggaattt ttccctaaca
     9841 atgctgttga atattttgtt tcttattatg attattatca gcctgaagct tatgtgcctt
     9901 caagcgatac gtatatcgaa aaagattcaa gtgtaaatga tgagatcgac aaattacgac
     9961 attcggctac tagttcatta ttagaaagaa atgatgtgat cgtcattgca tccgtttcat
    10021 gtatttttgg attaggttcg ccttttgagt accaaaaaca agtagtttcg attcgacagg
    10081 gagcagaact agatcgcaat cagttgattc gtgacttagt cagtatccaa ttcgaaagaa
    10141 atgatataga ttttcagcgc ggtcgttttc gagtgcgcgg tgatgtcgtg gagatttttc
    10201 cggcttcaag agatgaacgt gccttacgtg tggagttttt tggagatgag gtcgaacgta
    10261 tccgagaagt caatgcactg acaggtgaag ttttaggaga aacggaacac gtggcaatct
    10321 tcccggcgac tcactttgtg acgaatgatg aacatatgga gcatgccgta gcgaatatca
    10381 aagcggaatt agaacagcgt ttaactgtct tgcgaaatga aaacaaactt ctggaagctc
    10441 aacgtttaga acagcgaacc aattacgata ttgaaatgat gttagaaatg ggctacacgt
    10501 cgggaattga aaattattct cggcatatgg acggacgtaa agaaggtgaa ccgccttata
    10561 cgctattgga tttctttcct gaagattttt tgatcgtggc ggacgaatcg cacgtaacca
    10621 tgcctcaagt cagaggaatg tataatggtg accgagcgag aaaacagatg ctagtagatt
    10681 acggtttccg tttgccatct gctttagaca atcggccact tcggttagaa gagtttgaaa
    10741 aacatgtcaa tcagatcatt tatgtctctg cgacgccagg accatatgaa catgaacaaa
    10801 cagataccgt tatccaacaa atcatccgtc cgacagggct gcttgaccca gtgatcgaag
    10861 ttcgtccaat catgggacaa atcgatgatc tagtaggcga aataaatgaa cgagtcgaaa
    10921 aagaccagcg ggtgtttgta acgacattga cgaaaaaaat ggcagaagac ctcacagatt
    10981 atttcaaaga attagggatc aaagtcaaat atttgcatag tgatatcaaa acgctggaac
    11041 gaacagaaat cattcgagat cttcgattag gtgaatttga tgtgttagtt ggaatcaatt
    11101 tgcttcgtga aggattagac gtacctgaag tttcccttgt tgctatcttg gatgcggata
    11161 aagaaggctt tttacgtagt gaacgttcct tagtccaaac gattggacga gctgctcgga
    11221 atgaagaagg taaagtaatc atgtacgcag acaaagtaac agattctatg cgtctagcga
    11281 tggatgaaac atcaagacgc cgtacgattc agcagaagta caatgaagaa catgggatcg
    11341 ttccaaaaac gatcattaaa gaaatccgag atttgatttc tattacgaaa gaatcggaag
    11401 atgatacaaa agaagcggta caagtctcgt atgaagaaat gacgaaagaa gaaaaagata
    11461 cgttattgat gaaattagaa aaagaaatga aaaatgcggc gaaagcattg gattttgaaa
    11521 cagccgctaa tgtgcgagat atgattttag aattaaaagc ttcaaagtag gaggacaaca
    11581 tggctaacga taaaattacg attcacggtg cacgtgccca taacttgaaa aatatagatg
    11641 taacgattcc acgagataaa ttcgtggtcg ttacaggttt gtccggttct ggaaaaagtt
    11701 ccctagcatt tgatacgctg tacgctgaag ggcagcggag atacgtagag agcctttctg
    11761 cttatgctcg tcagttttta ggacaaatgg ataagccgga cgtagatagc atcgacggat
    11821 tgagtccggc gatttctatt gatcagaaaa caacaagtaa aaatcctcgg tcgacagtag
    11881 gtactgtgac ggaaatcaat gactatttac gcttgttgtt tgcacgtgta ggacatccga
    11941 tttgtcccaa cgatcatatc gagattacca gtcaatcagt agatcaaatg gcagatcaag
    12001 taatggcttt gcctgagcgc acaaagatcc aagtgttggc accgatcgtg gtaaagaaaa
    12061 aaggacaaca taaaaaaata ttcgaacgaa tccaaaaaga gggatacgtc cgcgttcgag
    12121 tcgatggtga gatctatgat ttaagtgaag tgcctgagct ggagaaaaac aaaaaacatg
    12181 atatcgcgat cgtcatcgac cggatcattg tgaaagaagg gattcgttct cgtctatttg
    12241 attctttaga ggcggctctg cgattggcag acggctatgc tctggttgac gtgatcggtg
    12301 aagaagaaat gttgtttagc gaacactatg cctgtcctta ttgcggattt actgttggcg
    12361 aactagaacc aagactgttc tcattcaatg cgccttttgg agcttgtcca gactgtgatg
    12421 gtttaggtgt caaattagaa gtcgatcaag atttagtgat tccagataga acgaagacac
    12481 ttagtcaagg tgctatcgtt ccatggaatc caatcagctc tcagtattat cctcaaatgt
    12541 tagcgcaggc ttgtgaaagt ttcggtatcg atttagatac accattcgaa gaattaccgg
    12601 aagagcatca ggatatcatc ttgaatggga cagatgatct tttccatttc cattatgaaa
    12661 atgattttgg tggcgttcgc gatgtagaaa cgactttcga aggggtattg aagaatatcg
    12721 agcgtcgtta tcatgatacg aacagtgatt tcacgcgtga gcaaatgcgt ctttatatga
    12781 cagaattaac ttgtcagaca tgtaaaggtt atcgattgaa tccccaagct ctatctgttc
    12841 aaatcaatgg aacgaatatc gggcaaacaa atgagttgtc tattcaaaaa gcagtagatt
    12901 tcttctcaaa cttaacgtta tctgaacaag agaaagtaat tgccaaaccg attttaaaag
    12961 aagtaaatga cagactttca ttcttggaaa atgtgggact agactatcta actttgagtc
    13021 gcgcttccgg aacattatct gggggagaag cgcagcgaat cagattggct acacaaattg
    13081 gatcgaatct ttctggagtt ttgtatattt tagatgaacc atcgatcgga ttgcatcaac
    13141 gcgacaacga tcgactgatt ggctcattaa agaaaatgcg tgatcttggc aacaccctcg
    13201 ttgtagtcga acatgatgaa gatacgatgt gggcagcaga ttatctgatt gacgtcggac
    13261 ctggggccgg agaacaaggc ggacaaatcg tggctgcagg cactcctgaa gaagtagcta
    13321 atgatgaaaa ttctttgaca gggaaatatc tttcagggaa aaaatcaatc cctgtaccaa
    13381 aagaacgccg taaaggtaat gggaaagcaa tcaagatcac tggtgcttca gaaaacaatc
    13441 tgaaaaatat tagtgttgaa tttccattag gagaatttgt agcagtcaca ggtgtgtctg
    13501 gttctggaaa gagtacattg gtcaatagta tcttaaagaa atcgttagcg caaaaattaa
    13561 ataagaattc tgctaagcca ggtaaattca agacaatttc cggctacgaa agtatcgaaa
    13621 agatcatcga tatcgatcaa agcccaatcg gccggacgcc gagaagtaat ccagcgactt
    13681 atacaagtgt atttgatgat atccgtgggt tatttgctca aacgaacgag gcaaaaatgc
    13741 ggggttataa gaaagggcgt tttagtttca acgtaaaagg cggtcgttgt gaagcttgtc
    13801 gcggggatgg aattattaag atcgaaatgc actttttgcc tgatgtctat gttccttgtg
    13861 aagtatgtca tggcaaacga tataactctg aaacattaga agtgcattac aaaggaaaaa
    13921 gcattgctga tattttggaa atgacagtag aagatgctgt agaattcttc aagcacattc
    13981 caaagattca tcgcaaactg caaacgattg ttgatgttgg cttaggttat gtgacgatgg
    14041 ggcaaccagc aacgacattg tccggtggtg aggcacaacg gatgaaactt gccagtgaat
    14101 tgcacaaaat ctctaatgga aagaatttct atatactaga tgaaccaacg acaggacttc
    14161 atagcgatga catcgcccgc ttgttgcatg tattacaaag attagtagat gctggtaaca
    14221 cagttttagt gattgaacac aatctagatg taatcaaaac agcagattat atcattgatt
    14281 taggaccaga aggtggagaa ggtggaggaa cgatccttgc gactggaaca ccagaagaaa
    14341 tcattaacgt aaaagaaagt tatacaggtc actatttgaa aaaaataatg gtataaagag
    14401 gatagatagc tgagatagtt aaaaaaacat aaaaaaatga atttttgctt gttttattaa
    14461 tatcagtgtt ctagaatcat tacatggtag ttctttctac actgattggt atagacaaaa
    14521 acttcctata agttcaatcc gttcaaaaaa gaattgagca atcggaggag tttcctttga
    14581 ttgctttttt ctttatggta tgatattcct tgtgtagttg gaaaagaaaa acaagagatt
    14641 atgagtaagt gagagataat gatgaacagt attgaaaatg aagaatttat ccctatatta
    14701 ttaggaagcg acatgaacgt ttatggtatg gcacgttctt ttaatgaagc atacggcaaa
    14761 atctgccaag catatgcttc ggatcagtta gcccctaccc gctacagtaa aattgtcaat
    14821 gtagaagtta ttccaggatt tgataaagac cctgtgttta tcgagacgat gcttcgtttg
    14881 gcaaaagaaa gatattctga taagagtaaa aaatatcttt tgattgcttg tggtgatgga
    14941 tatgcagaat tgatctccca acataaacaa gagctgtcag aatattttat ctgtccatat
    15001 attgattatt ctttatttga aagactgatc aataaagtaa gcttttatga agtttgtgaa
    15061 gaatatgatc ttccgtatcc taaaacattg atcgtcagag aagaaatgtt agtaaatgga
    15121 catttggaac aggaattgcc ttttgaattc ccagttgctt tgaaacctgc aaacagtgtg
    15181 gagtatcttt ctgtccaatt tgaaggaaga aaaaaagctt ttattttaga gactagagaa
    15241 gagttcgatt taattttagg ccggatatac gaagccggtt ataaaagtga aatgattgta
    15301 caagatttta tcccgggtga tgatagtaat atgcgtgtgc taaatgcgta tgtagatgaa
    15361 gatcatcaag ttcggatgat gtgtcttggt catccactgt tagaagatcc tacaccagca
    15421 tcgattggta attacgttgt gatcatgcca gattataacg aaaaaatcta tcaaaccatt
    15481 aaggcatttt tggaaaaaat cgaatataca ggctttgcca atttcgatat gaagtatgat
    15541 cctagagatg gagaatataa attatttgaa atcaatttga gacaaggtcg tagcagtttc
    15601 tttgtgacat tgaacggctt gaatctggct cgtttcgtca cagaagatcg agtgtttaac
    15661 aaaccctttg ttgaaacgac ctatggaacg aatcaatcag ataaagcaag attatggatg
    15721 ggtgtgccca aaaaaatctt tttagaatat gctcgggaaa atgaagacaa aaaactagct
    15781 gagcaaatga tcaaagaaaa tcgttacggt actactgtgt tttatgaaaa agaccggtca
    15841 atcaagcgct ggttgttgat gaaatatatg ttccataact atattccaag attcaaaaag
    15901 tattttcatg tgaaagaagg ctagttcatg gagaattttt tcagtatttt aggcggaatg
    15961 ggcacgatgg cgacagaaag ttttgttcgc ttgatcaacc accgaacgaa agctacaaaa
    16021 gatcaagaat atttgaatta tgtcttattc aatcatgcaa cagttcctga tcgtacagct
    16081 tatattttag atcgatcaga agaaaatcca atgccgtttc tgctggatga tattgagaaa
    16141 caaaatttat tgcagccgaa cttcattgtg ttgacttgta acaccgcaca ctactttttt
    16201 gaagaattac aagcggcaac ggatattcct atccttcata tgccaagaga agcagcaaat
    16261 gaacttgtcc gtcagcatac gacagggaga gtagcgattc taggaacaga aggaagtatg
    16321 aaagctggaa tctatgaacg cgaagtcaaa aatcttggat tcgaaacagt gattcctgat
    16381 actgttcttc aagaaaaaat caattatctg atctatcatg agatcaagga atcggattat
    16441 ttgaatcaag agctatatta cgaaatctta gaagaagcgg tagagcgcct aaactgtgaa
    16501 aaagttatct taggttgcac agaattgtca ttgatgcatg aatttgctga agataaccat
    16561 tatccagtga tcgatgccca atctatttta gcagatcgga cgattgaacg agcgctagct
    16621 gaaagaagca aggcattgga tacagcatcg gaaaagtaaa aaaattggat ttggggcaaa
    16681 tatcctaaaa gatatttggt ccaaatcttt ttgtaaaaga aaaagaaaac tggtaatgat
    16741 acgcccggaa tgatataatt ttactatgca caatgtgaga ggagttcgta attatgacag
    16801 ataatttgca attagtcatc ataacaggta tgagcggtgc agggaaaaca gtagctatcc
    16861 aaagttttga agatatgggc tatttttgta tcgataatat gccgcctcga ttgattccaa
    16921 aattctggga attaatcaaa gaatcaggca aagtcacaaa aatggcttta ggagtggatt
    16981 tacgttctcg ttccttcttt gaagaaatcc aaaacatgct gatcgaaatc gaaaatacga
    17041 acttcatcga cacttctatc ttatttttag atgcttcaga cgaagaacta gtggctcgct
    17101 acaaagaaac aagacgagca catccaatgg cgatggacgg tttgatcact gaagggatac
    17161 gtaaagaacg ggcgatacta gacgacttga aagcccaagc ttccgtgatc attgatacga
    17221 cgacattgac accacgacaa ttacgtgaaa aaatcaatga gtcattcaaa tcaaagggag
    17281 accaaggatt tcggatcgaa tttgtatcat ttggttttaa atacgggttg ccgatcgatt
    17341 cggatattgt gatggatgtg cgttttttgc ccaaccctca ttatatcccg gaactaagac
    17401 cattgaccgg aaaagatcag ccagtctatg attatgtgat gtcctttgat gaaacgcaat
    17461 cattctacca taaatttttg gatcttctgg aaactgtatt gcctggatac attaaagaag
    17521 gaaaaagcag cttgacagtt gcaatcggct gtacaggcgg tcaacaccgt tctgtggcat
    17581 tgactgaacg tttggcaaat gctttaggga aaaactataa agtcaatgtg acacatcggg
    17641 acaaggataa aaggaaagag acggtgaacc ggtcgtgaaa atgaaaacct accggataag
    17701 aaaaccaaag atcgtagtgg tcggcggtgg cactggtctg ccagttatcc tgaaaagttt
    17761 gcgaaatcaa ggagcagata tcacagcagt agtgactgtt gcagatgatg ggggaagcag
    17821 cggtgcaatc agagaatcga tcgctatggc tcctcctggt gacttgagaa atgtcttagt
    17881 ggcgttatcg gatatgccac aattctatga agatattttc caataccgtt tcaaaaagga
    17941 agatcagttt ttagccaatc atacgatcgg taatctgatc attgccgcag tttccgagat
    18001 gcggggaagc acttatgagg cgattcaatt attagcaaaa atgatgcatg tagatggaca
    18061 tgtttatcct tcttcagaaa ctcctttgac cttgcatgct gtctttaaag acggaacgac
    18121 tgcagtagga gaatctaaaa tcgcgatcga ccgtaaaacc attgaccgtg tgtatgtctg
    18181 caacacaaat gataaaagtg aagccaaagc ggctcgaaaa gtcgtttcag caattatgga
    18241 cgccgacatg gtcgttttgg gacccggaag tttattcacc agtatccttc caaatttagt
    18301 gataccagaa atcggcgaag caatgctcaa gacaactgct gaaaccgtct atatctgtaa
    18361 tatcatgacc caaaaagggg aaacggaaca tttcacagat gccgatcata tacgtgtttt
    18421 acacaagcat ttgaatcagc cttttatcga tacagtattg gtcaatacgg aaaaagtgcc
    18481 tgaagattat atggacccag agatctatga tgagtatcta gtacaagtcg cacacgactt
    18541 caacggattg agaaatgaag gatgtcgagt gatttccacc gactttttgg aattgaaaga
    18601 tggtggtgtc ttccacgatg gagacaaagt agtagaagaa ttgttccgtt tagttttcgg
    18661 ttcaaaatat taagcaattt attgttggag aaaggaggag aataggatgt catttgctgc
    18721 agatgtgaag aaagaactaa ctggtttgga agttcatcga gaacatgcga aagcggaatt
    18781 agctgctttg ctgcgaatga acggctcttt aggtattgtg aaccagcagt ttattttgaa
    18841 cgtccaaaca gaaaatgctg caattgccag acgtatctat tctcttttga aagatcatta
    18901 tcaggtacga agtgagctac tcgttcgtaa aaaaatgaaa ttaaaaaaga acaatgtcta
    18961 tatcgtccgg ttgaagcaag gcacaaaaga tgtgttgatg gatcttgata tcatggatgg
    19021 tatgatgttc aatggacacg tttcggacga gattatggga aattcgcaaa aaatgcgttc
    19081 ttacttacgt ggtgctttta tggcctcagg ttcagtcaat aatccggaaa cgagccgtta
    19141 tcatttggaa atattttcta tttatgaaca gcataaccaa gacatttgcg atatgttaaa
    19201 ttattacggt ctacatgctc gaacgcttga acggcgcaat ggctatattt cttatttaaa
    19261 aggagcagaa aaaattgctg atttcttaac attgattggt gcaaccaact caatgctccg
    19321 atttgaagat gttcgaatcg tacgggatat gagaaattcg gtcaatcgtc tagtcaattg
    19381 tgagacagca aatctgaaca agacgattga tgcggcttct aaacaaatcg aaaacatcga
    19441 attgatcgaa gcaaaggtcg gtctacatgc ccttcctgaa aaacttcagg aaatcgcaga
    19501 acttcggctt caacatccag aggtcagctt gaaagaatta ggggaaatga ttccctcagg
    19561 tgcgatatca aaatcgggaa ttaatcaccg aattcgcaaa ataaatgaat ttgcggaaaa
    19621 actgcgtgaa gaagcagttt aatccaaaga aaaaactgat acatgaagca ggagatgcga
    19681 atgtatcagt tttttttaaa agttatactt gcttttcttc cgtcaaacta taattccatc
    19741 tttactggag taacataatt tggaaaggct tctattgttt ttaatacaat cgtataaaaa
    19801 gcggggagac aactgaatga agattttagt tttgtacata aatttttgct taatatagaa
    19861 gtcctgatat agataagatc aagtatttat ctataactgg cctgattaat taaaaaactg
    19921 gaaggaactg ctcgatcatc cttgagaact gctcttctta cttctggtca agattctcgt
    19981 ggttggtgga taaggatggt tgataagttc attggattag ctagtcgaaa acaaccaggt
    20041 gtaattaatg tagaagtttt aacaagggga tggagtgatt tattcagtga taatcataag
    20101 ttcataaata gtaattgaga tgtatggttg gaatacgtta tacctgaact tgaactacca
    20161 agatttggtt tagaatttga gaatcgatgt ttataaaggg agggcaaaaa tgcgtttttt
    20221 tacaactttt actatcatta tgattgcagt actttttatt tttctagata tagcaaagcg
    20281 aaatacagca tttttattat atcgggtttt acttcgagca ggtctcataa catttatatc
    20341 gatcgttgga ttttttctat tcacagttat tgtatttatt tggagaactc cagcaccacc
    20401 attgccagag attacatatg gagaatttcc ttttagactt gagtatgaat tgaatgaaga
    20461 attacatgtg atagaagaca cgttaatagt tgaatttgat ggatttggaa tgaacgaagg
    20521 catagggaga tatcgtagat ggacaagtcg tttagccagc ggagaagatt tagtgttgct
    20581 gttagaagtt agtgataata agcaaatatt ttattttcca ggacctgcta attattacat
    20641 gggagatagg ttgaatggtt ataatcatac ttttcctagt gcttctttca ttgaaaggga
    20701 aagaggcatt actagaaggg atattttaca tgacaaagaa ttattagaac aatttggtcc
    20761 gcttgatcaa aatacaataa atgaagaaga attgttaaat caatataata taaggctagt
    20821 gaattgggaa atatctgaac cgattgttaa caattttggt gattagaaaa caattacgat
    20881 aaatagaaac aagccaaatc atcagcaagt aaaagctgat gatttggcct ttcttatacg
    20941 agtagtagga attcatagcg aatattatta cgatcagtag atattttgat attaaaggag
    21001 tattacgaac tagtatgtga gaatgtagag gtgatttgcg aataatgaga aatttatcaa
    21061 atagaaacaa aatattaatc attattgttg ttatagctgt gtttcatctg ggaacgaatg
    21121 cggttttaag tagaataata ttgggtccta agccaccaag acctgcgatt acccgagggg
    21181 agtttgattt tagattagaa tatgaagtag atggggaaag gattgtgata gaagatacaa
    21241 ttgttgcatt atttgatgga ttttctgcag atgcaggtag catggcatgg tatcgcacgt
    21301 ggcgtttgca tttggcgagt gacaagaggg cacaacaatt attgttacat gagttagatg
    21361 atggtcgaaa aatatattat cattttggag gagctggatt ttatatgggg aagttagtag
    21421 cagtgtaact gtaacaagga aagatttgcc aggtcttggc attcgaaact ccatatttta
    21481 tatcgagcgt gggagagata catctattgg gaacaggaaa gggttaactc ttgaagagtt
    21541 atataatgac tttggaatcc gtcttgttag ttgggaacac aatccaccaa tagaaaatcg
    21601 ctttgaataa tcgaaatcta ttaatccatt tcatcagcca gtgaatgttg taatggaaca
    21661 atttggttta gtgttcaaaa ccattgaaat actaggctgt gatcagcaaa aatgtatctg
    21721 aataaaaaag agagcaacta ttaaatgaga ggagttagga gttgttagct ttatacgaat
    21781 caaagtggca aatacagatg cgtatgcatt tgtgtttgcc atcttgcgtt tggtagacga
    21841 gataggtata agctaaagaa acaacttgtt taaaataaac gacctagctc aacaaacgac
    21901 catctaatca actaacagca tacataaaag caactatttt tgaagtagaa ttgaaatcgg
    21961 gataacttaa aaagtgattg ggatactagt gtgaatcacc gtacataaat attactcgtt
    22021 aagggaattt gagaaatttt gt
//
LOCUS       DAZKCY010000036        21738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000036 DAZKCY010000000
VERSION     DAZKCY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.036"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(375..1328)
                     /locus_tag="ACG8XC_001255"
     CDS             complement(375..1328)
                     /locus_tag="ACG8XC_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9190964.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1351..2106)
                     /locus_tag="ACG8XC_001256"
     CDS             complement(1351..2106)
                     /locus_tag="ACG8XC_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190965.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2103..3062)
                     /locus_tag="ACG8XC_001257"
     CDS             complement(2103..3062)
                     /locus_tag="ACG8XC_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9190966.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3055..4005)
                     /locus_tag="ACG8XC_001258"
     CDS             complement(3055..4005)
                     /locus_tag="ACG8XC_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9190967.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4155..4607
                     /locus_tag="ACG8XC_001259"
     CDS             4155..4607
                     /locus_tag="ACG8XC_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFP9190968.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4696..6963)
                     /gene="gshAB"
                     /locus_tag="ACG8XC_001260"
     CDS             complement(4696..6963)
                     /gene="gshAB"
                     /locus_tag="ACG8XC_001260"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFP9190969.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7150..8148)
                     /gene="ftsY"
                     /locus_tag="ACG8XC_001261"
     CDS             complement(7150..8148)
                     /gene="ftsY"
                     /locus_tag="ACG8XC_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP9190970.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8159..8968)
                     /locus_tag="ACG8XC_001262"
     CDS             complement(8159..8968)
                     /locus_tag="ACG8XC_001262"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9190971.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(8977..12558)
                     /gene="smc"
                     /locus_tag="ACG8XC_001263"
     CDS             complement(8977..12558)
                     /gene="smc"
                     /locus_tag="ACG8XC_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFP9190972.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12578..13264)
                     /gene="rnc"
                     /locus_tag="ACG8XC_001264"
     CDS             complement(12578..13264)
                     /gene="rnc"
                     /locus_tag="ACG8XC_001264"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP9190973.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13612..15393)
                     /locus_tag="ACG8XC_001265"
     CDS             complement(13612..15393)
                     /locus_tag="ACG8XC_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9190974.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15419..16333)
                     /locus_tag="ACG8XC_001266"
     CDS             complement(15419..16333)
                     /locus_tag="ACG8XC_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9190975.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16360..17322)
                     /gene="opp4B"
                     /locus_tag="ACG8XC_001267"
     CDS             complement(16360..17322)
                     /gene="opp4B"
                     /locus_tag="ACG8XC_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP9190976.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17322..18266)
                     /locus_tag="ACG8XC_001268"
     CDS             complement(17322..18266)
                     /locus_tag="ACG8XC_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190977.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18267..19271)
                     /locus_tag="ACG8XC_001269"
     CDS             complement(18267..19271)
                     /locus_tag="ACG8XC_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9190978.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            19593..20780
                     /locus_tag="ACG8XC_001270"
     CDS             19593..20780
                     /locus_tag="ACG8XC_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9190979.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            complement(20872..21612)
                     /locus_tag="ACG8XC_001271"
     CDS             complement(20872..21612)
                     /locus_tag="ACG8XC_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9190980.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
BASE COUNT         6050 a         4637 c         3833 g         7218 t
ORIGIN      
        1 cttattgccg agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa
       61 aatatctaaa tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga
      121 ggcgatcttg atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat
      181 caacccgtat gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa
      241 accatccgta aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat
      301 aggctcttgc tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc
      361 ctctgttatt tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat
      421 tctaaaccgc ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt
      481 ttaccagcat tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct
      541 ccaccaatag cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca
      601 tattcataag aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca
      661 aagccaaata aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc
      721 ccttcattca caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc
      781 tgatcgattg cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag
      841 atctccccta aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa
      901 gaaaggaaaa tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca
      961 gaaatgatga ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt
     1021 ccacctgctg attctacttt tttataggat gaaagatact caggaaggct gcttgtagct
     1081 gctccaacta ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg
     1141 aatacgacga ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt
     1201 atcgtcaatg tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg
     1261 ttgctatttc cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat
     1321 tttttcatct cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc
     1381 aatctcacaa atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat
     1441 ttcttgggtc gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc
     1501 ataacttgca gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg
     1561 tactaaattt tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa
     1621 aggttcatcc agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg
     1681 ttggagttga ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag
     1741 gtgttccagt gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata
     1801 aggaaatcgt ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga
     1861 ttgtcgtagg atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt
     1921 atggtctaaa tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac
     1981 cgttgacttt ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt
     2041 taactgtaca tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc
     2101 aatcatagga tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca
     2161 aattcaacga cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt
     2221 cctcctgtca gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga
     2281 taagtagaaa agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga
     2341 cccacaagag ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca
     2401 aaacgatctg tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct
     2461 agcacatgtc gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc
     2521 aaaagatact gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca
     2581 tattcattcg gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct
     2641 aaaatcatcc ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag
     2701 aaaaataaca atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta
     2761 cctgtctgct gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg
     2821 acatataacg actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc
     2881 tgaaagctga ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc
     2941 ttactcctca atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt
     3001 cctgtaacaa ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc
     3061 atatcgtcgt ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat
     3121 cacactgact ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac
     3181 taagaacaaa ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata
     3241 tattctagag acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat
     3301 caacatgact gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta
     3361 atctattccc agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc
     3421 aaatagccaa accaagagca acaatggaac aacagcatac aataactcat aatttcctcg
     3481 tatcacagtc gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg
     3541 ataagcaaaa aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg
     3601 aagcatcaat tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc
     3661 tgcaaaagca aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa
     3721 aatcatagcc actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga
     3781 aacaaattta ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc
     3841 cacaaggatc aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg
     3901 cccatctaac tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat
     3961 actacttaaa ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct
     4021 aattgagaat gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt
     4081 tcatatgttt taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa
     4141 ggagggtatt ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg
     4201 gtacaccttt agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct
     4261 gctatcttag tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa
     4321 aagaactcaa aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg
     4381 tccaaaacca atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg
     4441 attacgtcac tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa
     4501 atgaacctgt gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa
     4561 aagaaaaacc aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac
     4621 ataaattttt acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt
     4681 caaaagcttt ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa
     4741 tcttctgcct tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc
     4801 ttcgatgatc ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat
     4861 caaatcgata ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt
     4921 gtaagcttca gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga
     4981 gttctccctt agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg
     5041 ttctttcaac atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg
     5101 attcgttcca cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat
     5161 actgtcacca ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat
     5221 cacaaagaaa cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc
     5281 acgaaaagca atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat
     5341 cgtgatacct aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa
     5401 tctcggataa gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg
     5461 gaacccagct tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac
     5521 gatatagctg tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa
     5581 ttgcagacgc aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat
     5641 ggcatcgaac ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata
     5701 gggacgttca tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg
     5761 actcgatagc tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat
     5821 ttgagtcaat gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat
     5881 ttgcttcatt ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg
     5941 ttgttctagt gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc
     6001 ttctttttct tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc
     6061 tggattgata cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg
     6121 aacacctgtt gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata
     6181 aaattctttc tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata
     6241 ctgtttcata gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact
     6301 gttgcgaata ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc
     6361 actcattggt gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct
     6421 cgctgttttc aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg
     6481 gctgaacaat gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac
     6541 catctgtttt cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa
     6601 attttttaat tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact
     6661 taatggccag atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc
     6721 gagatactga aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt
     6781 ttcgctaaaa tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg
     6841 atcggttttt gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa
     6901 tcgagcttgg tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat
     6961 cattaagtga cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa
     7021 cgaaattgtt tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc
     7081 tatgttaaaa acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt
     7141 agacatgggt tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg
     7201 atcaaacggc tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg
     7261 caattcatta cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa
     7321 aacaagaccg gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg
     7381 accagtggtt gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg
     7441 gattactcgt tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc
     7501 tgcagtatca actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa
     7561 tacgacagct gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc
     7621 gccccagaca actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa
     7681 tactttttta ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc
     7741 tacaccatta acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc
     7801 atttacttca ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt
     7861 ttggacttct gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt
     7921 aattcgtatc gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc
     7981 aaaaaagtct tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa
     8041 tgttttacgc gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt
     8101 tttctcttcg ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct
     8161 actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca
     8221 cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag
     8281 catattggat catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt
     8341 tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca
     8401 taaattccaa aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg
     8461 gtatttcttt gattttagga tctaattctt cttggtcata agcgattact gccttattaa
     8521 gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca
     8581 acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag
     8641 acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg
     8701 atacttccat cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc
     8761 cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg
     8821 ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt
     8881 gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt
     8941 ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt
     9001 cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg
     9061 catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac
     9121 gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc
     9181 ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat
     9241 aatggaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag
     9301 actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc
     9361 agttggttcc gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat
     9421 tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc
     9481 catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt
     9541 tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac
     9601 tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat
     9661 atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg
     9721 cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt
     9781 ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag
     9841 cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt
     9901 taatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac
     9961 ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg
    10021 ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa
    10081 ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc
    10141 tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt
    10201 ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta
    10261 atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc
    10321 aaaacgttca agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa
    10381 acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc
    10441 ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa
    10501 ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg
    10561 tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac
    10621 cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc
    10681 taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac
    10741 ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc
    10801 cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg
    10861 ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg
    10921 ggctgcccca cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag
    10981 ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac
    11041 cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg
    11101 tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc
    11161 ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga
    11221 tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac
    11281 tgatgcttct acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat
    11341 atattgacgt tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc
    11401 aacatattga tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa
    11461 ttcttgctgt gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc
    11521 tgctgctttc atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc
    11581 tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa
    11641 aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg
    11701 atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga
    11761 ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg
    11821 tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac
    11881 atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc
    11941 ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt
    12001 gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac
    12061 accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc
    12121 tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa
    12181 ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt
    12241 ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact
    12301 attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc
    12361 aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac
    12421 ccagcggata gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt
    12481 gacgctattt tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc
    12541 aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt
    12601 ccgccgcagc ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat
    12661 catctacata aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca
    12721 atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat
    12781 gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga
    12841 atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata
    12901 ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca
    12961 aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga
    13021 tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat
    13081 actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg
    13141 ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca
    13201 aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt
    13261 ccattttcca cattcctttt atggatattt tttcgatact tccttattat actggttttt
    13321 tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg
    13381 acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg
    13441 gctttccata tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt
    13501 ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa
    13561 atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg
    13621 tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca
    13681 gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa
    13741 tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc
    13801 aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg
    13861 agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt
    13921 attccaagca gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa
    13981 agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca
    14041 ttgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa
    14101 gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt
    14161 gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat
    14221 ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa
    14281 acctgcatag aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg
    14341 taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt
    14401 ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt
    14461 atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc
    14521 atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat
    14581 ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc
    14641 tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg
    14701 atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata
    14761 tgaccagata ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc
    14821 aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata
    14881 ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt
    14941 attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga
    15001 ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag
    15061 cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg
    15121 agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc
    15181 tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc
    15241 attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga
    15301 gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat
    15361 cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact
    15421 agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac
    15481 tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta
    15541 agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca
    15601 agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg
    15661 tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa
    15721 aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg
    15781 tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa
    15841 aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg
    15901 gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt
    15961 agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc
    16021 cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt
    16081 catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt
    16141 tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga
    16201 tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa
    16261 attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt
    16321 ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg
    16381 gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa
    16441 aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag
    16501 ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga
    16561 agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg
    16621 actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga
    16681 tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc
    16741 ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc
    16801 accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa
    16861 aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta
    16921 gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg
    16981 cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat
    17041 ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc
    17101 ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc
    17161 tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa
    17221 tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg
    17281 cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct
    17341 ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca
    17401 tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg
    17461 tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag
    17521 atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg
    17581 tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat
    17641 aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga
    17701 acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca
    17761 acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta
    17821 tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct
    17881 tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc
    17941 tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa
    18001 cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg
    18061 gctgttacct tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac
    18121 tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca
    18181 tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg
    18241 tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa
    18301 tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct
    18361 tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga
    18421 ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt
    18481 ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac
    18541 agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca
    18601 gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct
    18661 tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc
    18721 gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca
    18781 cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta
    18841 ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc
    18901 tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat
    18961 gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc
    19021 gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg
    19081 ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat
    19141 tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca
    19201 tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg
    19261 ttatcggaca atcgaatacc ccttgtatat tagagttagg gttttaacct tccggttttg
    19321 attggataac cctatgaacc atcgttattt taatttggta ttaactcatt tcttttgaga
    19381 taatatattt attggattga gaggctaagc cgttcccccc ttgggccatt gaacccgcac
    19441 tcgagagact ggctcctctc tctgataagt ggattgcgta tgagttagat gttgaaagtc
    19501 cttactttac tccgtatctc gtacaaggta gtgacgcttt tcagttgagt ggatttcaat
    19561 ccaaaattct ttttttgaaa ggtcagatat acatgtactt tttaggcatt gatatcggta
    19621 aacgcactca cgttgcttct atcatgaacg aggaaggaaa ggtattacta aaaggttttt
    19681 catttcctaa caccactgaa ggagcagagt cattaattga gcgaatggtc gactattccg
    19741 gagcacccag tgactttgtt atagggatgg aagccacggg gcactattgg ctgtccatct
    19801 tttcatacct tcacgaatca gattatttga ttcacgttgt gaatcccctt caaacagacg
    19861 gatggcgtaa aggtacagaa atacggaaac gaaaaaacga tattattgat tctgttttga
    19921 ttgctgactt aatgcgctat ggctcatttg tcgaaacggt cttatccgat gaaaatgttt
    19981 tttctttgaa acaactttct cgttaccgaa cttatttagt aggtacagcg agcgacttca
    20041 aaagaaaaat tatagccgta ttggatcaag tctttccaga atacgcgacg atttttacca
    20101 agcagggcgt tttcggaaaa gcttctaaag agttattgct ggcgtttagt gccccgatcg
    20161 acttagaaaa catttcctca gaaactttgg cccaatactt agctgagtta agcaggaacc
    20221 gaatcaaggc agaaaaagcg gaccaattga agcaagcagc ttctaactcc tttggagtaa
    20281 aatttgctca agaagcgttc accttccaac tacgttcaat gattgaacaa ttaaaattca
    20341 ttgaaagcca aataaaggaa acggaaagag aaatcaagca cataatggac actttaaatt
    20401 ctgttattct cacaattcct ggaattggtc ctataaacgg tgcgactatt cttggtgaga
    20461 tcggagatat tcaaaaattt tctaacccga aaaaactcgt tgcttacgct gggatagacg
    20521 cttccgtgac acaatcaggg caatatgagg caacgcataa tgttatgagt aaacgagggt
    20581 ctccatattt gcgaaaagcc ttgttttcgg cggctttagt agcttctcaa tgtgatcctg
    20641 tttttagtgc gttttaccag aaaaaagttt ccgaaggaaa acatcatcta actgccttag
    20701 gtgcggtttc tcgtaagctt tgttacgtga ttcatgctat tttgactaaa aacgagcctt
    20761 atataattgt ttctgattag ctattgaaca ttcaaaaaaa ctaatggtaa gaagcttatt
    20821 tgttgtaccc ttttttcgga attatctgtt ataaaatatt ttttgatttg gctaattttt
    20881 acttgactct atatagttag tctctccaat aaatatagta tctccatagg ctttttttaa
    20941 attctcttca ttaaatacag attctacact tccgtgagca attagatttc gattcaaaag
    21001 aataatatta tcaaaatact tctttacctt gcttaaatca tgatgcacaa ttagcactgt
    21061 ttttccctga tgtttcaatt cttttaacgt atccataatt attcgttcac taactaagtc
    21121 tatccctaca aatggttcat ctaaaaatat gaaatccgca ttttgagcaa gacatcttgc
    21181 taaaagtaca cgttgaaatt gtcctccaga taattctcct atttgatgat tagaatattt
    21241 tcccatatcg accttttcca aagcctttga aactctttgc cattctgcat ttttaattct
    21301 ttgaaaaact ttcattccgg catatgttcc catagataca cattctttta cttttattgg
    21361 aaaagtaaaa tcaatatcag ctttttgctc tacatatgca atcttcacca gttttttatt
    21421 gatatcttca ttattaatgg agacctctcc ctgatgaggg ataatattta acattgcttt
    21481 aagcaatgta gattttccag caccatttgg accaataata cctgttaaag caccttcttg
    21541 tatttttaaa gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat taagcttttt
    21601 cacttctaac ataactttct ccttctaaat gataatgatt atcacgatca tatggtatac
    21661 aaaaattata ttttagtcaa ccctaaattt tataatttag atttttttct gttgctgaag
    21721 attagaatga caaaacta
//
LOCUS       DAZKCY010000037        21703 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000037 DAZKCY010000000
VERSION     DAZKCY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.037"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1252
                     /locus_tag="ACG8XC_001272"
     CDS             <1..1252
                     /locus_tag="ACG8XC_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190981.1"
                     /translation="IYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1397..2137)
                     /gene="truA"
                     /locus_tag="ACG8XC_001273"
     CDS             complement(1397..2137)
                     /gene="truA"
                     /locus_tag="ACG8XC_001273"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9190982.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2328..2963)
                     /locus_tag="ACG8XC_001274"
     CDS             complement(2328..2963)
                     /locus_tag="ACG8XC_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFP9190983.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3110..4330)
                     /gene="sstT"
                     /locus_tag="ACG8XC_001275"
     CDS             complement(3110..4330)
                     /gene="sstT"
                     /locus_tag="ACG8XC_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP9190984.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4549..5547
                     /locus_tag="ACG8XC_001276"
     CDS             4549..5547
                     /locus_tag="ACG8XC_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190985.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5743..7578
                     /locus_tag="ACG8XC_001277"
     CDS             5743..7578
                     /locus_tag="ACG8XC_001277"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9190986.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7638..8354)
                     /locus_tag="ACG8XC_001278"
     CDS             complement(7638..8354)
                     /locus_tag="ACG8XC_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFP9190987.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8581..9123)
                     /locus_tag="ACG8XC_001279"
     CDS             complement(8581..9123)
                     /locus_tag="ACG8XC_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9190988.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9128..9355)
                     /locus_tag="ACG8XC_001280"
     CDS             complement(9128..9355)
                     /locus_tag="ACG8XC_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFP9190989.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9434..11314)
                     /locus_tag="ACG8XC_001281"
     CDS             complement(9434..11314)
                     /locus_tag="ACG8XC_001281"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9190990.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11503..12117)
                     /locus_tag="ACG8XC_001282"
     CDS             complement(11503..12117)
                     /locus_tag="ACG8XC_001282"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9190991.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12259..12819
                     /locus_tag="ACG8XC_001283"
     CDS             12259..12819
                     /locus_tag="ACG8XC_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9190992.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13124..13438)
                     /locus_tag="ACG8XC_001284"
                     /pseudo
     CDS             complement(13124..13438)
                     /locus_tag="ACG8XC_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(13692..15327)
                     /locus_tag="ACG8XC_001285"
                     /pseudo
     CDS             complement(13692..15327)
                     /locus_tag="ACG8XC_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15882..16445)
                     /locus_tag="ACG8XC_001286"
     CDS             complement(15882..16445)
                     /locus_tag="ACG8XC_001286"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9190993.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(16460..17503)
                     /locus_tag="ACG8XC_001287"
     CDS             complement(16460..17503)
                     /locus_tag="ACG8XC_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9190994.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(17636..18040)
                     /locus_tag="ACG8XC_001288"
     CDS             complement(17636..18040)
                     /locus_tag="ACG8XC_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP9190995.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(18273..18908)
                     /locus_tag="ACG8XC_001289"
     CDS             complement(18273..18908)
                     /locus_tag="ACG8XC_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFP9190996.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(19174..20226)
                     /locus_tag="ACG8XC_001290"
     CDS             complement(19174..20226)
                     /locus_tag="ACG8XC_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9190997.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(20239..21177)
                     /locus_tag="ACG8XC_001291"
     CDS             complement(20239..21177)
                     /locus_tag="ACG8XC_001291"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9190998.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         6857 a         4284 c         3434 g         7128 t
ORIGIN      
        1 tatttataca ttttccgcct atcgtgccat tgacatattc catcgtgctt cattgggaga
       61 agctgtcgct ttaacatttt tgcctttgat actaatgggg tgttacgaaa tttatattag
      121 agattaccaa aaatggtact ggttgtctat tggcatgacg ctagtagtgt atactcattt
      181 actttctgtc gctatggttt ctgtatttat tggaggaacg ttgtttcttt ctttttattt
      241 ttgggaccaa aaaatcgcta gactcttaag tcttttaaaa gctaccgtcc tcaccttttt
      301 tttaagtgcg ggattcttaa ttccttttat tcaacaatct agagcacaag aattgaaagt
      361 cccactggga aaagaattaa gtggtatggc tcctagtgat atgctgacac atattttaaa
      421 taataactat aacaactata ctattggatt attcttattt ctcggattga ttggtgcttt
      481 tatttttata aaaaaattaa ctgctgatga tttatttatt ttctttcttg gtgtatttgt
      541 tcttttttgt tcaaccaatt tatttccttg gcaacttttt aaccatacac cagttaagag
      601 tctacaattt gtctggcggt taaatggttt ttcttcttta tttatagcct atacgatgag
      661 tatagtgatc tattatattt tttctactaa aaataattca tggaaaataa tggtctttac
      721 gtttcttgca ctaatattgc atctctcagg agtaagcaat tctatccatg ctaacaaaga
      781 ttcattaaca cttatagcac ctgaagacgc tgttgctata gctgaaaatt acaatcatac
      841 tgattatgca aacaaagagt caatctatca tcctgatatg attaataatg atcaatattt
      901 actaggtaac caggaaataa atccaacaac tcattttatg agtaacaaac ttacaattga
      961 attagataat tcgaataata aaaatactgt cttgacgaca cctatttatc gttataaagg
     1021 gcaagtggct tctattaatg gcaaactagt acaaacaaaa ttatctaagt ttggcacaac
     1081 tgaattaact attcctccag gtataaataa agtcgttatt acttatcagt acacaaaact
     1141 agcaattgct tcaagatatt tatctatcgt tactttgatt ttatttttac tttatcgatt
     1201 tactttttct aaacaaaaac aaccgagaag agaagtacaa cattctcact agatttttat
     1261 ttaagaaaga ctttttagct gtacacataa acaggctaaa aagtcttttt ttatatttct
     1321 ttaatttgct tcatacatta tttaacatcg atatttatac tgtgaaacat gcgtttcatc
     1381 cattatcaaa gtatttttat cgataataaa cttcatccaa aaacagacct tgtgcaggga
     1441 cggtttctcc tgcatgttgc cgcactttgc tttcaaatat ttcatcgatt gtttctatcg
     1501 gcatggcacc tgtgccgatt tctagtaatg tccccatgat aatccgcacc attttatata
     1561 agaatccgtc tccgacaaaa gtaaaatgaa gcatttctcc ctttcgttcg atagataatt
     1621 catcaatcgt acgcactgtg gatttttttg attttttcaa tgcagaaaaa ccaatgaaat
     1681 catgtgtacc cactaatttt tcgcaagctg cattcagctt gtccatatcc aactcttgag
     1741 ggaaataaaa gctgtggttt cgctcaaatg ctgaaggaat cacgttgttc caaacataat
     1801 agctgtattt tttgcccacc gcattatatc ttgaatggaa gcgctcaggc atttcttcga
     1861 ttcttttgat gacaatatcc cgcggaaggt aacgattcat aaagtcgatc atttctgcca
     1921 gtccaatttc tgtgtttgtt ttaaaatttg ctacttgtcc tcgcgcatgg gtgccagcat
     1981 ctgttcttcc tgatccaata atttctatct cttcatttgt catctgatgc aatacttttt
     2041 cgatttttcc ttgaatggtt ttatcgtcat ctccaagtct ttgccatcct gaatatcttt
     2101 ttccgtcata ttcgattgtc aacttgatgt ttctcatatt ttagtggctc ctctgtcttc
     2161 catttggtct tctctctacc tttttaagat tgtagcacaa aatatagctc cttgcttctc
     2221 taagacagaa gctatacaag aaaactgtgg aaatatatat ttcagaaaaa caaaaaacag
     2281 ccaccggatg aagtatccgc tgactgttca atttgacggc tcaagtttta ctgtttgaga
     2341 tgcaagatac tcaaccaggc aatgggcaca taattaaaga aaaaactgat tctttggcaa
     2401 aaacctattt tattcagtaa tggccaagaa tttatagaag gaattcttgc cagtccataa
     2461 atcgccgcaa aaagcaaact gatcacagac agtccaaggt ataagtggct tacgtccatt
     2521 tgcccatttt ttcgatagat caagaaaaga agaactggaa acaataaaaa accactgtaa
     2581 cctaaacctg atccgatatt atggacccat gtcgagacat tccacgaaga ttcatgggta
     2641 ttgatactga acaaacctgt aaagatacaa tcacctatac catagaacgc gatggctgct
     2701 gtaactaggg tagctaacac acgagatgta ctaacaaatg cagcatacaa tgcaggcaat
     2761 gacaggatga aaaagatccc tgaaaccaca gaccaaataa gaaacgcttt tcttacagga
     2821 ctggaaacct ctccaaatac actgatcacg ctcttcattt gatctaattc aggataatac
     2881 aatcctaata cgtacggagt caaaaaatca ctcagcacgc ctaataataa aaagaaaaaa
     2941 ccaaatttac gtaaaaaatc catgatacgc ctccaataac aattacctat gcttttaatt
     3001 gttattataa acgactttat cttgatttaa caaaaacctc tatttacacg aacatctata
     3061 taactcatta aaaactcgaa tcattttaaa aatactaaat atctaacgtt caacgttcac
     3121 ttaatccatc taaacaatct ttcaatttga ttttttcccc acttctttta cgcgcgccta
     3181 gctctcctat tgcagtaaac aacagatcac ttgacgaatt taaggcagtt tccattgaat
     3241 cctggataac accgataata aatccaatac cgacgatttg catcgcgaca tcattagaga
     3301 taccaaacaa actacatgct aatggaatca ataacagtga accgcccgca acacctgaag
     3361 ctccacaagc agctaaagca gacaaaatac ataaaaggac tttcaaaaca aaaggtgctt
     3421 caatccctaa agaatgtaca gcagtcaacg tcatcaagga aatggtaatc gcggcaccgc
     3481 ccatattgat cgtagcacct aacgggatcg atatggaata agactctttt gtcaatttca
     3541 atctttcagc caacatcatg tttatcggga tatttgcggc agaacttcgg gtaaagaaag
     3601 cgggaagtgc gctttctttc aaacaaaaga aaatcaatgg atagggattt tctttaataa
     3661 acaaaaacgt gaggaatggg tacacaacta atgcgacaaa cagcatcgta ccgatcaaaa
     3721 caagtaaaag ctgcagatac tctgccaatc cagcgattcc agtcgtagca attgattgat
     3781 aaaccaatcc taaaatccct atcggagcaa atgcaataat cacttgtacg acttgtgaca
     3841 acgctataga tatctgatcc accaactctt ttgtcgattc tgaacgtgct cgaaaagcta
     3901 gtcccaacaa tacagcccag aataagacgc caaggtaatt cgcatcaata agagattgaa
     3961 caggattttg tgtgacattc gtcaacatag tagataaaac actttccaaa ctttgcggtg
     4021 ctttttcaga tactgtttct tgtaaagtaa taggaacagt aaaaattgaa cttacaataa
     4081 ctgctacaag tgcagaaagc aatgttccca ctaaatataa gatcaaaata ggcttcacga
     4141 atgtcttatt cccaatttta tgcttggcaa ttgacgccat cgtcaagaaa aaaaccagaa
     4201 taggtgcgat tgccttcaag caactgacaa acaacgttcc caatattgaa ataaatgtcc
     4261 atgtggggaa aaagatacca aacaatattc cgactaaaat accaacaaca atacgttgaa
     4321 ctaacgacat atccaaaaac ttctctaaca aatgaccacc ctttcacaat tttaacaatc
     4381 attgtatcat tttatgcaaa gaaactaaag gtataatttt ctaaaaattc taacaataca
     4441 aacaaccatt tcttgctaat tttgtacttt ttctgtctac acgttgtttt ttgagatatt
     4501 ctaagttagg atgttattaa tgggcaactt atcaggaaac gttttcttat gtttttgtcc
     4561 aatttttttg tgaggtgttt attcttgaaa aaaataaaga aggagagtta tggcttcctg
     4621 acaatctttg tcgcttgtat aatgatggtt ttacagcgta tattgatttc agcagtcact
     4681 attcctacat atgcaacaaa ccctggtcga ttctacatct atacattttt acaaacttcc
     4741 gttgttattg gtgcaaattt gatcccactt tacatgggtt accaatatca aaaaatcgcc
     4801 cgtttaaaag ttttacatta tttaagtcgt tttgccttta tattcctaat agccacacta
     4861 gtttgcaata tcttttttta cgtacaagct ggcagcttga atattcgtga ttattggctt
     4921 atcctaacac ctatcagtca gaattacttt acttatgctg tttcttgtac tttgcttttt
     4981 tgtagtattc cttatacagt tggattttta aataaattat cgaaaacaac aattaaatat
     5041 ctactattat ttttaacaat tggattagtt ggctgctcta ctctatttaa taaagacccg
     5101 tttgcattac agaatggaca gagtatttta tggatttttt atttattttt aatgggttat
     5161 ggtttgaaag aatggaactg gaaagaaaag gtccgctata agcttcctca agtatttatc
     5221 agcttgattg ttttattcgg tttgattatt ttgatgacac aaatttcact catcatcaga
     5281 ggaaatacag atacagcgct gaggttttct aaaccatatt ctctcttcgc aatgtattat
     5341 actttctctt cgtttttatt tttagaaatg ctatctgata aattaggtat aaaaggaaga
     5401 ggaaattttc ttgctgtatt tttgattgtc actcaagtta tatgtaattt ccctttagtt
     5461 tcttatcgcg tatctacatt tatgaaacgt gaatttccaa actcagggaa ggcatggctg
     5521 ataaatatcg ggcagttttt tatttgatat ttattagcag tgtccctcct agttatcttt
     5581 ttattttata tacaaaaaat ccgtttcatt catcgtttca tcgaatactt cgcttttgat
     5641 tcgattgaac aattaactca gcgttggaaa aaaagaattc attggctttt tgttcataaa
     5701 agttattttt tagttgcagc atttttttac tgctttacct tcgtgcagat tttcgtttta
     5761 gaaccaaaag aaggttggaa agaaactatc caggttgcct taaaaatatt tactcagcga
     5821 caagcaccca tgatcctgac aatcatcatt attatgggat ttttcttctt actattgttg
     5881 atttccaatc gtttttggta cgccttgact gctaccttga tcattaactt actgctcact
     5941 atttcgacag taatcaaaat ggaaatgcgg gaagagcctg tctttccttc agatttaaaa
     6001 atgctgaccg gattgtcaga actattatcg atggttagcc cagtcttatt aatcgtgggc
     6061 ggattgatcc ttctcctatt attgattact agtattattg tacaacgtag actacaaaaa
     6121 cagtattcct tgaagattca ttggaaacgt cgatttatca gtatcgctgt attattagga
     6181 tgtttttcgg gtgtcttttt cattaatcac aaaaattcgc cttctttctt gctgtttaat
     6241 ttatttaaag taaacaagac tttctttaac cagcaaggcg ccgtaaaaga taacggacca
     6301 gtcattcaat tcctaaacaa tatcgacatt aaaatcatgg aagaacctgc tggctattcg
     6361 aaagaaaaaa ttaatggaat aatgaaaaag tacaacacaa aggcagatga aatcaaccaa
     6421 acaagaagtg actggttaaa caatcagacg atcgttctca atttaagtga aagcttcagt
     6481 gatcctgcac gtgtccctaa tctgacagta cctacagacc ctattcctta catcaaaagc
     6541 attataacag aaggaacagg aggattgatg ttatccgtcg gttatggtgg cggaacagct
     6601 aacatggaat gggaaggact gacaggcttg agtatcagta acctgtctgc ttcgttagtt
     6661 acaccttata cccagctagt ggagcgtcag aaaataagtc caaatattac cgatctattc
     6721 gatgaaaaaa tagccatcca tccttttaca gctgctcttt atcgcagaaa acaagtgttt
     6781 gataaatttg gttttgacag gttctattat ttagacagtc cggataaatt aacttatacg
     6841 gataaaatag aacaaagccc acgcatatcg gatgattcag catacaaaga aaccttgaaa
     6901 gctttacaaa gcaatacgga aacgtcacaa ttcatccaat tatccactat gcaaaaccat
     6961 atgccttatg atgattacta tagtgaattg aactacactg ctgaaggaac tgctgtcatt
     7021 cctaatagaa gaaaagaatt acaaacttat atgcaaggcc ttcactatac cgatacagca
     7081 gtaaaaaaat tcatccaaga aatcgacaaa atcgataaac cgattacctt tgttttttat
     7141 ggtgaccatt tacccgcaat ctatagtggg aatgattcga aaaaatatgg attagaacag
     7201 caccagacag attatttcat ttataacaac aagtatagtc gtgaacaagc agctacgtta
     7261 aataaagaag ttgttgctcc taataacttt ggtgctatgg ctcttgagca tgcgaatatc
     7321 aaagtaactc cttactatgc gttgttgaca gaagtatcta atcatctacc tgcattaaca
     7381 attgatccta cagaaagctt gtctaatcgt tttaatggaa aacaggtctt tgttacagag
     7441 gatagtcaaa ttatccaaga aaaaaattta actgagaaac aaaaagaaat tctagaagat
     7501 tatcgtctga tacaatatga tcttgttgca ggtaaccagt acagtgcaac atgggcgaca
     7561 caaaaaataa aagactaaca aataaactga gcaaggggtt gtgataaaag acatgtcaca
     7621 accccttgtt tatctcatta atttgcttgt ccataaacga tttgattaaa gccatttttc
     7681 aattcattat tcgctgtctc gttgaagtaa agataattat cgggattccc ctgattttgt
     7741 tgtgtcacca atacttttgg ctgattttgc tgaaacccga atagatagac gtgctttttc
     7801 agatatggtt ctgtctctgc atcggaatag acggctgtga tttgatagtc tgcttgacct
     7861 gtcccacttt ctgaccattc tgcagaaaca ggcactccac caatagccat cttccactcc
     7921 ccgttcaaaa tagcttgagg gactttaaga ccatataagt ccacactcat ttgattcgta
     7981 taacttttgt attgttgtcc cattgtttgg ccccatttgg tcataaatgc ctctaattgt
     8041 gcggatttgt ctgcattcca cagtgtttgt tcaacttgtt cgctgctttc tgaagaggaa
     8101 gatgaagcct ctacagaaga acttgaagta gatgtttgag ttgtttcagt cgttttggaa
     8161 gactctgacg aagtagaggt atctgctgtt tctgtacttg ctgcagttga cttgctctct
     8221 gtgctagttg ttgtagtagt tgcaactgtt gctttcgctg tttcttttgt tgattctttt
     8281 ttcgattcat tcccgttggt acaaccagct aacaaaaaag ctagaccaat tgttactaaa
     8341 atcctttttt tcaattaaaa aactcctttc gattactccc acttctttat tctatcaaat
     8401 aagcaatgtg attttattga tttttcgtat tttctcatca atctttcaaa tcttctaaaa
     8461 actgagaagc acccgctgat gttgtctgta atacatcagc gggtgcttct tttattttaa
     8521 tcttcgtgat tttccaattt acctcacgct ttatatttgt atttgaagaa acatagactt
     8581 ttagtcatca tcgtcttcct catccaaatc ttgtcggaca gtttttcctt ggagtctttg
     8641 tacttggtag atgatttctt tattgaaact taaaagttca acaagcaatt gttgcaagcc
     8701 atatttttct tctttttcag ctaatttgat tgcccgtgtg ataaagacat tttggaaatc
     8761 aaaatctttt gcaaagtttt ccaacattgt tgaggctggt aaatatttgt tttgaccatg
     8821 ttcgtaaatt tttccataac gcatgacttc ttctaaagta gacggaacga tttcattttc
     8881 aatcactaac tcatgagaga ttttattgaa ccatctcgct tcttgatcag catattctga
     8941 aaaaagggta cttaattctt gcgaagatgt acccaaaaca tacaattgtg cttggatcaa
     9001 tttcatttct tctaatttaa aatttgctaa tacatgcccc accatggcgc cagcagttgg
     9061 tttatgatgg tcgatatctg cttgttttgt ttcttgaaca aatttttctt ccactatatt
     9121 cattgattta cgcctctttt acttttgctg attggacttc ataacctaat ttttccacaa
     9181 ctgcagctaa ttgttcaggg tggacttcat ctggatcaaa gtttgctttg attttgctcg
     9241 cattaaacaa aactttcgta ttgcttaccc caggctgttg agaaaccgca tgttcgattt
     9301 tttgcataca gcttggacaa gctagtgtat ctaattgcat aataattttt ttcatcgtag
     9361 ttcttccttc ctttttcttg atagtcttat tctagattac agcttccata aaaaaattga
     9421 ttcttatcaa gttttacgat ttttcttctt tgctttcgtg tccaaaatac aataaacgca
     9481 tcgcgttaaa gataacgatc aagatactag cttcgtgaac gaacatccca ctagccatgt
     9541 aaataaaccc ggcaaataag ccgttgagta aaaataagac gacaccaatg gcaatcacta
     9601 cattttcacg ggtatttaaa acagttttct tagccaagcg gtaggcatga accaacgatt
     9661 caaaattaga ttgcatcaag acaacgtcac tcgtttcgat agcaacatcc gttcctgatc
     9721 ccatagcaat cccgatatca gccgcagcca aagagggaga atcattgatt ccatctccaa
     9781 taaaagcaac gcgcaatcct tgttcttggt attttttcac aaattgaacc ttttcgtctg
     9841 gtaataattc agcatggact tcatcaatgc ctaacatatc agctacataa tcagcagtca
     9901 tctgattatc acctgtcagc atcactaact gttttgctcc tgcttgtttc aacttagcta
     9961 attgttcagc gacttctggt cgaatcacat ctgacacacc tatgatttga gtgatttttt
    10021 tgtcgattgc tacaataatg accgagctgc ctgtttgttg taattgatgt aaatcttgta
    10081 cttgctttgg tgtgagagaa agtttgtgag cggcaatcaa tttggtgttg cccgcacaaa
    10141 ctgtatgacc atcaatctca gctgtgattc cttgtccctt gattgtctga ttatctttga
    10201 ctgctaattg ttgaaaatca atgtctttct cctgagcata atggatcacg gcgcgcccca
    10261 gaggatgatc ggataacgtt tctaatcgag ccactaaagc taagctgtcc ccttcatcct
    10321 ctgtatagtt cttgatagtg gatacagcag tagttccttt ggtcaatgta ccagttttat
    10381 caaagacaaa agtatcgatt ttggaaaaag tatccattgc ttcaccgcct ttgactaaga
    10441 tgccccgttt cgctccatta ccgattcctg ctacattcga aacaggcgca ccaatgacta
    10501 aagcgccagg acaaccaaga accaaaacag tgattgctaa acgaaaatca cgactaatca
    10561 gaccaacaac caacgcaata agtaaaacgg ttggagtata atactgagaa aatcgatcaa
    10621 tgaatttttc cgcatgggat ttggtatctt gtgcttcttc tactaattcg ataattttgg
    10681 caaaggtcgt atcatctccg accttagttg cttcaatcgt cacgtagcca tctgctaaaa
    10741 tcgttccaga gaagacttca tctccactga ttttgacgac ttgtcgtgat tcaccagtca
    10801 ctgcggcttc atccgcatac cctttcccgt caatgactcg tccgtccaca ggaatagctc
    10861 caccagtttt tactaagacg atatcgcctt cttccacgtc atcgatttcc acttcttcag
    10921 tcgttccatc ttcttggaca acactagcag tagatggggc catctcggtc aaagccttga
    10981 ttgactcccg cgttttggtc aatgtttttt gttccagata gctaccaaac aaaaacaaga
    11041 atgtaacgat agctgactcc ttgtattctc cgatcataaa ggcaccaagc acagcaatag
    11101 taacgagtaa atcaattgag ataactttga ctttcactgc ctgatacgca tggatcataa
    11161 taggtatgac cgcaataata gaagctatgg ctagagcaat gttatagaac aagacaaaac
    11221 cagtaaattt tccgataaaa ccaaaaataa tcaacacagc tgataaggcg gtgatatgat
    11281 ttcgatgctc ttggatccac ttttgtaatt tcatctttct ccctcttttc ctttcattct
    11341 tacgagacta agtatagata cttatccatc ataaaaaatt gaccactatc aagtttagtc
    11401 aaaaaggcgg ttttcttttg gtagaataca ctacaaaaaa tagttagcaa gaagactcac
    11461 taaaagtgat cttgccaact attgtcgttt ctatcgaaat tttcattgaa tgattttcct
    11521 gtacagcata catcggagat ctatttcttt atctaagatt tttatcttgc taattttttc
    11581 ctttagtctt tcaaatccac gtcgttcata gaattgatac gtacagccac tatctgtaaa
    11641 aaggaatatt tcttttcctt tctctctttt ttcaaattct gttagcaact tggtaccgat
    11701 gccttttgtt ttcaaatcag gatttgcagc taaaaaaatg atctctccat ctggcgaatg
    11761 cttctttaaa tatgctgcaa gcatttgttc atttgctgtc tcgtaagctc ctttactttc
    11821 tttagcaaat atattttgta aaaaatcgaa tatcttaaca tatatttgct gtccaaaaga
    11881 acgatgtttc tttttttctc cttttatctc cgccagcaaa acacctgcca actcgtcctc
    11941 tgcatacaaa gcaagaattt gagttgctcg attcaactcc atatacaaaa aatatcttcc
    12001 gtaaagattc agcagaagtt cattttccat atatctctca aagttcattc cttcaacagc
    12061 aaaatagata gcttttttgt aatctttctt ttctaagggt ttaaaagtta gtttcatcat
    12121 ttcgcctcct caacttcaat gattgtgtta tacttacatt ataatagaca cagtgtctac
    12181 tatctataat caatcatcta taagtttgtc gattaagcga cagctttgct ttgatatgtc
    12241 ggataaggag ataggtaaat gaaaaagaca gatagacgcg tcaaaaaaac agaaaaagct
    12301 ttggctgaaa cactatccac attattagta aataaaaaga ttcaagcaat cactatacgc
    12361 gaactaacag aaacggcaga tgtacatcgt tcgacttttt atacacatta taaggatatc
    12421 tatgatctat atgatcaatt agaaagcaac ttttttcggg atctaaatga aattctctct
    12481 tatgatccag cccatagcta tgaggaacta tacactcgct tgattgacta tgtttacgca
    12541 aatacttttt tatatcgctt atttatcctc aaccatggtg atatttcgtt tcagaagaaa
    12601 gttaaacaag ttattgaaga aaactatctg aacatatggc tatatgaaga caagaaaacg
    12661 attatcacag aagaaatgag attctttact acgtaccacg tccaaggttg tctatccgtt
    12721 ctcaatcggt gggtagaaag caattacaag tactccaaaa aagaattatt agaactctta
    12781 cagaaactga atgatcacat agagaaaatc atgccttgaa taaaaacata ctaagcttag
    12841 ttaagaagaa ccgtaacctt ctcgtataac aactgaatct tatttagcca aattgtttac
    12901 tgaagatttt ttgagtaaaa tttctataat tcctacttat tgaaaagtag tttgtagtgc
    12961 tgcttctccc tctctaagtg atatatcttg ttgtaccaat caaattcacc tcatcattaa
    13021 taaattactt ttaggctaat ttattacaac ttataggttg aagctgtaac caatttatag
    13081 tcatattata tttttatcat ccagtttatt gaattcttct cgtttacctc aattcatttt
    13141 caaggggaat cagctcatca tctaacattt tacgccatgc tcgcttcatt tcttgtgggt
    13201 atttaaatgg tttcgcttct ggatgtttgg ctttatatcc ttcgtaatcc ttactgttga
    13261 taattgcttt catattagga attggatctt cacctggcat atattgcaga ttagaagcat
    13321 gtaatttttt ctcagaaaca aaccactcat tggaaaagac atgaatcaaa tcatcactta
    13381 cttgtgtaaa atagcgacgt ttaatgacaa agatatcttc attgctatct agtgtacctt
    13441 catttctttg tgcttaaatt caaagataat tttctcaata aagatttttg attccagtaa
    13501 gattccttta ctccttttac aacatagcgt tgtcctttgt tttgaaacat cttcttcccc
    13561 tctttgccat taaaaatagc ccaattctct ataaagaaga ttaagcttca ttattataat
    13621 ataaagttta aatttggttt attacttact ttttcttatt ctctagtaag ctaatattga
    13681 aaaaaatgta atcagatcat tatttttata tgttgtaact tcctgataag aaaactaatt
    13741 aaaagaaaga ggtaactaca taaactaatt aaaaaagcta acttcatagt tggaggataa
    13801 atggaagaaa cttcaatgat tgtttctcct tgtgttcctt caatcgacac tagtcctatt
    13861 atcaatctct cttttttgtc catttacttt tgcgactgtt ccccaataat caaatacagg
    13921 aagcgttatt ttttgtcctt tagattcatg tactgtatag actattttcg aatccatcac
    13981 ttgataaggc atagtatgcc attccttgcc aattcttact tcatgattct gcaaatgttc
    14041 tttgtattgt aatgcttccg tgggtgcata gtcataaagt cctccgggaa ggagtgtcgt
    14101 gtaacgaata agattttctt gattcattac caagtgatct ctctgagtga caactttatg
    14161 ttcatagttt gcttttgttg cagcagatac agccaacagt aataaaacaa ttgccgttat
    14221 attaactact tttctttgct tagctgatgt gctcttcatg aaaacgaacg ataaaatcaa
    14281 cgagccaaac agtacttgaa agcctaagat tcgccaagga aattgaatca tttgaatggg
    14341 tgtattttgt aataaagacc atggaaaaaa tttggtggat aagagccaag agactaccgc
    14401 actaaggtaa atcacttttt ctttgcgatt aaatccttta aaaaagaaga gtggacagat
    14461 caaagaaata ataccaatga ttcctaccgt aaaggttgtc aggtcattat tcatcgcacc
    14521 aatcaaacta tccgagacat ttaatgcttg gtgttgtaag gtcgttttat ctggctcatt
    14581 aatcgattga accatgattt gtcggaacat cggataccaa aagtatgctg ttaaaagaat
    14641 cgtcattcct actgcctttg acaactccac aatcaaccgg atgctttctt tttttcttgt
    14701 ttcacttact acaaagccgc ctaagactag taagccaacg gctattacac tcataaaagt
    14761 ggaaagaaca tgtgtataaa tgagtaagct catactgact gctaatggaa ttgcacatgg
    14821 ctttttctca taaatcagtg cgtaagcata atacataact atcggtataa ccgtaagtgc
    14881 aatcgcttca gcaatagcag aacgatgata aatatcaatc gtacggtacc cataaaatgt
    14941 atataaacag ctaaatataa aagccgcatc gattcttttt aaaaatttta gtccataatg
    15001 aaacatcagc atgatagtac aaacattcaa tagccaaaca tataagatat aactaacgat
    15061 taaattatta cttatccaat aaaaaatgac tgccggaaaa aaggtcaagt agggataaaa
    15121 ataattcact ccgctcccat aatgattaaa cgtcgtaaaa ttgattggac cagaaaaaat
    15181 tgaagataac cctttgactc tagcaaaatg gaaggcaata tcccccgatg tagaaatagt
    15241 atgaacattt ctaaaataat tgattgaata aagtccagca ataattgcta ggagaatgga
    15301 caatattatt tttttcttta cttgcattac ttaattatat actcctttca tggattgctt
    15361 tatctagtat aacttaaatt ttaaataaat cgttatttat ttttactctc acttaaaaaa
    15421 gcactaaaat tataagagag ggaggtaatg gtctgaacac caagaattgt tccaacatcg
    15481 aattgcttaa ctaatgagtt caaagcttaa ttatgtggct cttatattta ccgagcgtgg
    15541 attgcgatct ctttagcgaa acaaacatct ttcataaaaa aaacagccat atccgctttc
    15601 ttggatatga ctatttacta ctactttact atctttcatt cttcttgtct cacttatgat
    15661 aaggcgctgc gtatcatgag tgaatgatat taggaaaact tcataaaata aatcttttat
    15721 acatttccac ttctagatgg tcattcatgt tagaagaatc aaactatttt tttactgtcg
    15781 taataatatt aaaacactag aacaaatcaa ttaaattcca taaaatataa ataatagacc
    15841 tagcatcatc tggtataaaa tggatgctag gtccgttata tttactcaaa attgattcta
    15901 cgaatagatc ttcttatcat ttctatttta gtagtcatgc gttgttgttc ttctaaaggt
    15961 ggtaatggta taagtagttt agataaattt gtaccattta catttggttg cccagttccg
    16021 taagaaattt tttctaataa cttccaatat aatggcgagt ctaagaagca gtctacgtat
    16081 tctgataata atttctcaac taacctaatt cgaatcaagt aagaagcaaa aacagattcc
    16141 tcacttattt ccttaaccaa gaaacttttt cccatagttc ctcctgtcct tgctatcaaa
    16201 atgtcatttt cttccaatct taaatcaact agtttagaat tactaatatc acagtaaggc
    16261 acactggacc aatttacacg tccctcttgt atatctgtga ttctcaaaaa cttaacattg
    16321 ccttgttttt tcgccgaatc tgtatagcca tattgaatct tagtagaaat acttcctaaa
    16381 taaacccata gtcgaacatc gctagatgat ttttcttgtc gttctttatg aacaacagtg
    16441 atcatcttga gttgtccgac tattttttag ttgcagcaat ctttttggga cgcttatttg
    16501 catgaagcaa cgttgctaag tctagtaata aaacattggc taacacagga ttggctttct
    16561 cgcgatacgc tgcggcgcca ctttcaaaca atactgctag acgatcttct acaacaacga
    16621 tttgttccaa atacggtgga aatttgattc gttttaggat ccggctagga gagtcccgct
    16681 cggacactaa gagttccgag gtataaggac cataagaacg actggctaag agataatggc
    16741 cataaaaagc cacaccttga atcttatcag atgtttttct gctcattgtc acggttggtt
    16801 ggattttgtc tttgcggcga attgctgttt tcatttcagc tatcgtcggc aatagataga
    16861 aagtcaactg tccttcacct tttagtgtga agttgccaga taccagttga tcattgtttt
    16921 gagtcaaata cgatgcttgt ggaatctctg agagatcagt tgtcagccaa taagcgattg
    16981 gctgtcccgt ctcttcagat gtatctgcta agatatcgat caaacgtaaa gcggctactc
    17041 gtccagtagc agatcctgtg attgtcaacc ataaaagttc ccgttttgta tcatatacta
    17101 agccccctac atgtggccgt ttaggtaaaa tcagcagttt gatcggctgt cctgtttctc
    17161 tatccagtac atgaataata gaatgatgct gatggtcata gcagtaggca gaaataaaca
    17221 caaaatcttg agaaaaagcc aacccttgcg gcgtcatcca ttgacaaacc tcattttcgc
    17281 cggtttccgg caccaccatt cttgtttctg ttaatccagg aatgttatat tgttttgctt
    17341 cagccttttc ccaagctgct tgaacaatag gataccgttt taactttttt tcaatatcct
    17401 gatgtatccg ttgctgcttc caatgcttaa actttaggac atagtaaaca agaaggccaa
    17461 tgaccagcaa cactaaaatc attattgcta cccgctgtgt catttttttg cctccttgtt
    17521 ctattcgatc aatcgtccat ataccgctat taattaccgt aactacatgt ttcttttact
    17581 agcgtttctt tataggatct ttcataatct catttatagt aacattaaga atcccttatt
    17641 ctactatagt cacttccaca tcaatattgc ctcttgttgc tttagagtat gggcaaaatg
    17701 cgtgggcttc ttctgcgatt ttctctgctt ggtccttctc aagtcctggc aaagaaactt
    17761 ctaaaacgac cccgatttgg tagcccccat cttctaattg aaataaagat acccgtgctt
    17821 tgacttcaga atcagactca agattatttt cttccagcat gtgttgaacc gcgccgttga
    17881 agcaagatgc gtatccagcc gcaaaaagtt gttccggatt tgtcccttca ctatggattc
    17941 ctgggcggtc gatcttcatg tgcatacttc cgtttggtgc ctcaacttcg ccatcacggc
    18001 ctccacgatt gatcattgct gtttcgtaca tttttttcat tgagatcatc ctttctttcg
    18061 tcgttctata ttaagtgtaa cgagtatttt ttaatctgac aaaggtaatg cttgcattcg
    18121 gcgacatcag ttagacaaca tcgatgatca gtgatagaag cagtttatct atacaaaaaa
    18181 agaagggttc tatgatattt ggtgtcctta ctcgccttga gtaacaacac caaatatcat
    18241 agaaccactt aatcagcttc acactgcttt ttctactttc gtagttgaaa aaaacttaaa
    18301 caaatcatcg gcaaatagtt aaagaaaaaa cttaatcgtt gaaagaagcc caattgctta
    18361 aagaaggcaa actgactaat cgacggaatc cgtgctaatc cgtaaagacc tgcaattagt
    18421 aaactaataa ctgttaaaac aagataaaaa tggctaagcg taccttgacc actttgacga
    18481 taaagcagaa caagtaacaa tgggaaaagt agaaagcctg catagcctag cccagaacct
    18541 atattatgga tccaagtcga tagattccaa gagctttcat tcgtattgat actgaataag
    18601 ccagtaaaaa tacaatcacc tatcccatat aaaccaactg ttgcaacaac aagtattgca
    18661 agtgtttttg atgttccaac aaataggtga tacaacgcag gcaacgaaag aacaaaaaaa
    18721 agtccagata caactgacca gaccaagaaa gcacgtctga ctggactatc tacgtcacca
    18781 aacacactaa ttacggtagt catttgattc agtttgggat aaaaaaggcc aaggatatac
    18841 ggtgtcgata aatcactcat cacacctaat aataaaaaat aaaaaccata tttttgcaaa
    18901 aaaatcattt tcttccccta ctttttagtg attattcgat acatccaaca atcaaatttt
    18961 ttctcatttt aacacatatt tttattctag ttgtcttttg aacaatcaat aacataaagg
    19021 tgagtttctt ctacatttta aaaaatcgaa tggcactgaa gagttcagtt gtcagcatca
    19081 atctcctact caaaaaggca aaaaaacgta agtcagatgt tgttgacatc tcacttacgc
    19141 atccattgat tgaaaatttc ttagtaaaat ctactagcct aaaaatttga agacttcttc
    19201 tcgatcatac agtttaaata ctttctcgca taaaaattga gtaattagtg ccgcaaaaac
    19261 agggatgact aaccaacaaa gaacaacaag aaagccattt agccctgcat ctaaagaagc
    19321 caacggacca acaagtccga ccacgccgaa accagcagaa gcaggcgtac ctgaaataga
    19381 aaatagagcg acaggaatag ctgtaatcac tgcactaact aaacaaggaa taagaatcac
    19441 aggttttcta aatagattag gcatcatcat cttcatagcg cctaaagcaa tcgcaattgt
    19501 cacacctgac ttattaacct tccaagagtt cactactaaa acaaaagtgg ttgcagctac
    19561 gcccatcgca gccgcaccag cagaaacacc atctagttgg attgccattc cgattgctac
    19621 tgttgaaata ggagagataa tcaaaaaggc aaaggaacaa ccaatcaaga tagacattaa
    19681 cagcggttgt aaggtagtga agttattaat caatgatcca attgcagaag taattgctgt
    19741 tacaaaaggg aataaataaa aaccaagtaa acctgcacct acacctacaa caatcggtgt
    19801 taacacaatc gacactgacc caaaactatt tccaatcagt aaaatcagcc cgacagcaat
    19861 cgatgctgtc agcatcgtat taataatatc gcctgtacca gcaccaacgt aaacacccgt
    19921 ttcacccaaa tcaggtgtga atttgactac accagaacca acaaaagaag ctccggctac
    19981 aaccatcatt ctcatcggat caaatccaaa ttgtaaggca atcaaaccac caatgatcaa
    20041 aggtgttgct agttgaaaaa taactgctaa ctgtgcgatc gttaccgcaa agggatacgc
    20101 actaaaatat tttaaaatgg cacctaatac tgcattagga atcaatccaa taatgattcc
    20161 tgttgctgta ccagcgagga ctttattcag aaaaattttc ggtgatattt tttgtgaagt
    20221 ttccattcaa tctctccttt attttacgtt caaactatct tcttttgcat ggacaagctc
    20281 agtcaagaaa tcacaataag gcgtcgcaac accatatttc ttcccttttc tagaaattgc
    20341 gccattgata taatcgattt ctgttaatcg atggttctta atcaagtctt gatacattga
    20401 aggataatgt aacccaattg tttccgggtc aaaacaagat tcacaatgtg caatgacttc
    20461 tgggacatct agttcaatct tctctactgc tgctactttg gcaaactcat tgacaatcgt
    20521 cgccaccatt ttatgagcag tcgatgtttt tcctagctca gccatattaa cgtctaagat
    20581 tgtgcatagt ccgttcattg ttccgttcac acatgctttg cgataaatag aatagtgaat
    20641 attgtcagaa aaatgagcat ttaatcctga ttcagacaat ttgtctgcta atttttttgc
    20701 agctgcttct tttccatcac ctaaattttg taattctact gaaccacttc caaataattt
    20761 gacctgacca ggaccttcaa gacctgccgt ccacatggta tttccaatat agatattttc
    20821 cattggtaca aatttctcaa taatatcttc atgcccgata ccatttaata gacataagac
    20881 ctctgtatct tttttgatta atgattggat atcctgcagc attttttcca gctgcatcgc
    20941 tttggtaaat agaataatca gatcaacttg atcttctttt tctacttcgg attgaagaac
    21001 gattggtagt tttgcttcta cctctttacc attaaaattt gcttgtaatc catgttcttt
    21061 gatttgttgg acatgttctg cccagccatc gattaataat acttcgttcc cactttggtg
    21121 aagcatcaac ccaaagcgac ttcccattgc gccagcgcct gcaattgcta ttttcattga
    21181 ttcttcctcc tttttctttc tattatactc atttgagaat cttttcacaa cctcgttttg
    21241 ttattcttct cttatttatc atgtttatat ccaccattca agttatttta tcagcaaatc
    21301 aataaaaact ggaatagaat cccaatttca tcgtaagaga aactagacta gcaataagaa
    21361 aatagaaaca ttcatagaaa aaatctcact cctctgatta taaaagaggg ggcattaacc
    21421 tcgtgtatac taaaaccttc cattcattcg atggatgatt ttctttttgt gaaaaaatat
    21481 tcattgtagt acgatgttgc gcaacacaga cggatggtat tgcttattat ttattaaaat
    21541 cgaacaatac acttatttaa aaacttcttt cccagatttt cttccatttt tacagttata
    21601 actagtatta tcatgaaaaa tagcctttta ttgtctatgg gaataataat ttaaaattgg
    21661 tactttttta ttaattagtt catcgcccaa taatctctcc agt
//
LOCUS       DAZKCY010000038        21116 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000038 DAZKCY010000000
VERSION     DAZKCY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.038"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            176..484
                     /gene="rpsJ"
                     /locus_tag="ACG8XC_001292"
     CDS             176..484
                     /gene="rpsJ"
                     /locus_tag="ACG8XC_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP9190999.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            515..1144
                     /gene="rplC"
                     /locus_tag="ACG8XC_001293"
     CDS             515..1144
                     /gene="rplC"
                     /locus_tag="ACG8XC_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP9191000.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1172..1795
                     /gene="rplD"
                     /locus_tag="ACG8XC_001294"
     CDS             1172..1795
                     /gene="rplD"
                     /locus_tag="ACG8XC_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP9191001.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1795..2085
                     /gene="rplW"
                     /locus_tag="ACG8XC_001295"
     CDS             1795..2085
                     /gene="rplW"
                     /locus_tag="ACG8XC_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP9191002.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2124..2957
                     /gene="rplB"
                     /locus_tag="ACG8XC_001296"
     CDS             2124..2957
                     /gene="rplB"
                     /locus_tag="ACG8XC_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP9191003.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3000..3278
                     /gene="rpsS"
                     /locus_tag="ACG8XC_001297"
     CDS             3000..3278
                     /gene="rpsS"
                     /locus_tag="ACG8XC_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP9191004.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3301..3648
                     /gene="rplV"
                     /locus_tag="ACG8XC_001298"
     CDS             3301..3648
                     /gene="rplV"
                     /locus_tag="ACG8XC_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP9191005.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3662..4318
                     /gene="rpsC"
                     /locus_tag="ACG8XC_001299"
     CDS             3662..4318
                     /gene="rpsC"
                     /locus_tag="ACG8XC_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP9191006.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4321..4755
                     /gene="rplP"
                     /locus_tag="ACG8XC_001300"
     CDS             4321..4755
                     /gene="rplP"
                     /locus_tag="ACG8XC_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP9191007.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4745..4933
                     /gene="rpmC"
                     /locus_tag="ACG8XC_001301"
     CDS             4745..4933
                     /gene="rpmC"
                     /locus_tag="ACG8XC_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP9191008.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            4958..5224
                     /gene="rpsQ"
                     /locus_tag="ACG8XC_001302"
     CDS             4958..5224
                     /gene="rpsQ"
                     /locus_tag="ACG8XC_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP9191009.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5282..5650
                     /gene="rplN"
                     /locus_tag="ACG8XC_001303"
     CDS             5282..5650
                     /gene="rplN"
                     /locus_tag="ACG8XC_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP9191010.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5687..5995
                     /gene="rplX"
                     /locus_tag="ACG8XC_001304"
     CDS             5687..5995
                     /gene="rplX"
                     /locus_tag="ACG8XC_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP9191011.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6023..6562
                     /gene="rplE"
                     /locus_tag="ACG8XC_001305"
     CDS             6023..6562
                     /gene="rplE"
                     /locus_tag="ACG8XC_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP9191012.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6581..6766
                     /locus_tag="ACG8XC_001306"
     CDS             6581..6766
                     /locus_tag="ACG8XC_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFP9191013.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6802..7200
                     /gene="rpsH"
                     /locus_tag="ACG8XC_001307"
     CDS             6802..7200
                     /gene="rpsH"
                     /locus_tag="ACG8XC_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP9191014.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7232..7768
                     /gene="rplF"
                     /locus_tag="ACG8XC_001308"
     CDS             7232..7768
                     /gene="rplF"
                     /locus_tag="ACG8XC_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP9191015.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            7929..8285
                     /gene="rplR"
                     /locus_tag="ACG8XC_001309"
     CDS             7929..8285
                     /gene="rplR"
                     /locus_tag="ACG8XC_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP9191016.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8306..8806
                     /gene="rpsE"
                     /locus_tag="ACG8XC_001310"
     CDS             8306..8806
                     /gene="rpsE"
                     /locus_tag="ACG8XC_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP9191017.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8821..9000
                     /gene="rpmD"
                     /locus_tag="ACG8XC_001311"
     CDS             8821..9000
                     /gene="rpmD"
                     /locus_tag="ACG8XC_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP9191018.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9044..9484
                     /gene="rplO"
                     /locus_tag="ACG8XC_001312"
     CDS             9044..9484
                     /gene="rplO"
                     /locus_tag="ACG8XC_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP9191019.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9484..10779
                     /gene="secY"
                     /locus_tag="ACG8XC_001313"
     CDS             9484..10779
                     /gene="secY"
                     /locus_tag="ACG8XC_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP9191020.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10841..11488
                     /locus_tag="ACG8XC_001314"
     CDS             10841..11488
                     /locus_tag="ACG8XC_001314"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP9191021.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11679..11897
                     /gene="infA"
                     /locus_tag="ACG8XC_001315"
     CDS             11679..11897
                     /gene="infA"
                     /locus_tag="ACG8XC_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP9191022.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            11930..12046
                     /gene="rpmJ"
                     /locus_tag="ACG8XC_001316"
     CDS             11930..12046
                     /gene="rpmJ"
                     /locus_tag="ACG8XC_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP9191023.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12064..12429
                     /gene="rpsM"
                     /locus_tag="ACG8XC_001317"
     CDS             12064..12429
                     /gene="rpsM"
                     /locus_tag="ACG8XC_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP9191024.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12457..12846
                     /gene="rpsK"
                     /locus_tag="ACG8XC_001318"
     CDS             12457..12846
                     /gene="rpsK"
                     /locus_tag="ACG8XC_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP9191025.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            12926..13864
                     /locus_tag="ACG8XC_001319"
     CDS             12926..13864
                     /locus_tag="ACG8XC_001319"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP9191026.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            13892..14272
                     /gene="rplQ"
                     /locus_tag="ACG8XC_001320"
     CDS             13892..14272
                     /gene="rplQ"
                     /locus_tag="ACG8XC_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP9191027.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14544..15383
                     /locus_tag="ACG8XC_001321"
     CDS             14544..15383
                     /locus_tag="ACG8XC_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9191028.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15359..16228
                     /locus_tag="ACG8XC_001322"
     CDS             15359..16228
                     /locus_tag="ACG8XC_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9191029.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16218..17015
                     /locus_tag="ACG8XC_001323"
     CDS             16218..17015
                     /locus_tag="ACG8XC_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFP9191030.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17218..17964
                     /gene="truA"
                     /locus_tag="ACG8XC_001324"
     CDS             17218..17964
                     /gene="truA"
                     /locus_tag="ACG8XC_001324"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9191031.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(18297..20966)
                     /locus_tag="ACG8XC_001325"
     CDS             complement(18297..20966)
                     /locus_tag="ACG8XC_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFP9191032.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         7018 a         3863 c         4549 g         5686 t
ORIGIN      
        1 ttataatgac aaaggtgcgt ttactttagt gaattgcaca gacgtaatca gaatgatttc
       61 gtcggctaag aaacgagagg ttgcgacacg cccggacgct ttgccacgaa cgagcgtgac
      121 ggaaattttc gtggagctat gtctactttt caaaataggc gaaggaggga acaagatggc
      181 aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac cgtattttag atcaatcagc
      241 ggataaaatc gtggaaactg caaaaagaac tggagctagc gtgtctggtc caattccatt
      301 accaactgaa cgcagtcttt acacaatcat ccgtgcgact cataaataca aagattcacg
      361 cgaacaattc gaaatgcgta cacacaaacg tcttatcgac attgtgaacc caacaccaaa
      421 aactgttgat gctttgatga agctagactt accatctggt gtaaacatcg aaatcaaatt
      481 ataaaactaa aacaaaaata atggaggtgt actcatgacc aaaggaatct tagggaaaaa
      541 agtgggaatg acacaaatct tcactgaatc tggtgaatta attccagtaa ctgttgtaga
      601 agctactcca aacgtagtac tacaagtgaa aacaatggaa acggatggct acgaagctat
      661 tcaagttggt taccaagata tgcgtgaagt tttatcaaac aaacctgcga aaggtcatgt
      721 tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa ttcaaaaatg ttgagctagg
      781 agaatatgaa gtaggaaaag aaattacagt agatgttttc caagcaggag acattattga
      841 tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt atcaaacgcc acggacaaag
      901 ccgcggacca atggctcacg gttctcgtta ccatcgtcgt cctgggtcaa tgggtccagt
      961 tgcacctaac cgtgtattta aaaacaaacg tcttgctggc cgtatgggtg gcaaccgcgt
     1021 aactatccaa aacttggaag ttgtacgtgt cgatgcagaa agaaatgtta tcttgatcaa
     1081 aggtaatgtt cctggagcta aaaaatcttt aattactatc aaatcagctg tgaaagctaa
     1141 ataattgtag gaagaaagga ggaactaagg aatgccgaat gtagcattat tcaaacaaga
     1201 tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc ttcggaattg aacctaatga
     1261 aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca ttaagacaag gaacacacgc
     1321 ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa ccatggcgtc aaaaaggaac
     1381 aggtcgtgca cgtcaaggat ctatccgctc accacaatgg cgtggaggtg gcgtagtctt
     1441 tggaccaaca ccacgttctt acagctacaa acttcctaaa aaagttcgtc gtttagcaat
     1501 gaaatctgta ttgtcagaaa aagttgctga aaacaacttg gtagcaatcg aaggcttgaa
     1561 cttcgacgca ccaaaaacaa aagaattcaa acaagttctt gctaacttat ctatcgactc
     1621 taaagtattg gttgttctag aaaaaggtaa cgactttgct gctttatcag cacgtaactt
     1681 accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg ctagacgttg tatcaaatac
     1741 aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag gaggtgcttg cataatgaac
     1801 ttactagacg taatcaaacg cccagtgatc actgaaaaat caatgcttgc catggatgac
     1861 aagaaataca cttttgaagt ggacactcgc gcaaacaaaa ctttagtaaa acaagcggta
     1921 gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg tgaacgtacg tgcgaaattc
     1981 aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc gcaaagcgat cgttacattg
     2041 actgaagatt caaaagaaat ccaattattc gaagctgctg aataagcact aatagataac
     2101 tcattaaatt aggagggaaa aacgtggcga ttaaaaagta caaacctacc tcaaatggtc
     2161 gtcgtaacat gacaggttct gattttgctg aaatcacaac atcaacacct gaaaaatcat
     2221 tgttacaacc attgaaaaac catgctggac gtaacaacaa cggccgcatc actgtacgtc
     2281 atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga cttcaaacgt aacaaagata
     2341 acgttgttgc aactgttcaa acgatcgaat atgatccaaa ccgttcagct aacatcgcgt
     2401 tagtacacta tgaagatgga gtgaaagctt acatcttagc acctaaagga ctagaagtgg
     2461 gtatgaaact tgtttcaggt ccagaagcag atatcaaagt cggaaatgct cttccattag
     2521 aaaacattcc agttggtact gttatccaca acattgaaat gaaacctggt aaaggtggac
     2581 aattgatccg ttctgctggt acaagtgctc aagtacttgg taaagaaggc aaatacgtat
     2641 tgatccgctt gaactctggt gaagttcgta tgatcttagc tgcatgccgt gctacaatcg
     2701 gttctgtagg taacgaacaa cacgaattaa tcaacatcgg taaagctgga cgctctcgtt
     2761 ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa ccctaatgat cacccacacg
     2821 gtggtggtga aggtaaagct cctatcggac gtaaagcacc agtttctcct tggggtcaac
     2881 cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc agacaaactt attgttcgtc
     2941 gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca ttttgagagg aggttcacca
     3001 tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca tttaatgaaa aaagttgaag
     3061 ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg gtcacgccgt tcaacagttt
     3121 tcccaagttt tgttggatat accatcgcag tttatgatgg acgtaaacat gtaccagttt
     3181 acatccaaga agacatggta ggacacaaat taggtgaatt tgcacctaca agaacttatc
     3241 gtggacatgc tgctgacgat aagaaaacga aacgctaatt tgaggggagg ataaatccaa
     3301 atggcagaac aaatcacatc agctaaagca actgcaaaaa cagttcgcac ttcacctcga
     3361 aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg tcgcagatgc aatttcaatc
     3421 ttgaaattca caccaaacaa atctgctgga atcattgaaa aagtattgat gtcagcagtt
     3481 gcgaatgcag aaaataactt tgacttagat gttgaaaact tggtagtaag cgaagctttt
     3541 gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg caaaaggatc agcttctcca
     3601 atcaacaaac gtacaagtca tatcacggta gtcgtatcag aaaaataagg agggataatc
     3661 agtgggtcaa aaagtacatc caattggaat gcgtgtaggc atcatccgcg actgggatgc
     3721 taaatggtat gctgaaaaag agtatgctga gttcttacac gaagatttaa gaatccgtaa
     3781 atttatttca actaaacttg ctgatgctgc tgtatcaaca gttgaaatcg aacgcgcaac
     3841 aggtcgcgta aacatttcaa ttcacacagc taaaccaggt atggttattg gtaaaggcgg
     3901 ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta actggtaaaa gaatccatat
     3961 caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa ttagtaggtg aaggaatcgc
     4021 acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa aaacaagcca tccaacgctc
     4081 aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct ggtcgtctaa acggagcaga
     4141 tatcgctcgt tcagaaggat actcagaagg aactgttcct cttcatactt tacgtgcgga
     4201 cattgattac gcatgggaag aagcagatac aacatacgga aaactaggag ttaaagtgtg
     4261 gatctatcgt ggagaaattc ttccaactaa aaaaaacact gagaaaggag ggaaataatc
     4321 atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc gcggaaaaat gcgcggggaa
     4381 gctaaaggcg gaaaagaagt agcattcggt gaatacggtt tgcaagctgt tgattcacat
     4441 tggatcacaa accgccaaat cgaagctgct cgtatcgcaa tgactcgtta catgaaacgt
     4501 ggtgggaaag tatggattaa aattttccct cacaaatctt atactgccaa agcaattggg
     4561 gtacgtatgg gttctggtaa aggggcacct gaaggatggg ttgcaccagt aaaacgtggt
     4621 aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag ctcgtgaagc gttacgtcta
     4681 gcttctcaca aattaccaat gaaaactaag atcgtaaaac gtgaggaaat gggtggtgaa
     4741 tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc gaaatgcttg atcaagaaaa
     4801 acaattaaaa gaagaattgt ttaatcttag attccaatta gcaacaggtc aattagaaaa
     4861 cactgcacgt attaaagaag tacgtaaatc gattgcacgc atcaaaactg tgttgcgtga
     4921 acaagctaag taattatgga aggaggccat taagcgtatg actgaaggaa gaaatcaacg
     4981 caaagtttat caaggtcgcg tggtttcaga caaaatggat aaaacaatta ccgttgctgt
     5041 cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg aactattcta agaaatacaa
     5101 agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc gtgagaatta tggaaactcg
     5161 tccattatca gctacaaaac gtttccgttt gttggaagta gtcgaagaag cagttattat
     5221 ctaataacac gagattttcc tatatagatt gaatgcaaaa tctgaaagga ggatacacaa
     5281 cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac tcaggcgcac gtgaaatttt
     5341 gacgatcaaa gtccttggtg gatctggtcg taaaactgct aacatcggtg atgtgatcgt
     5401 tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa aaaggtgacg tcgttaaagc
     5461 cgttatcgtt cgaacaaaat caggagctcg tcgtacagac ggttcatata ttaaatttga
     5521 tgaaaatgct gcagtgatta ttcgtgatga taaaagccct cgtggaacac gtatctttgg
     5581 gccagttgca cgtgaactac gtgaaaacaa cttcatgaag atcgtttcac tagcaccaga
     5641 agttttataa tcttgagaca cttatcaaag gaggtgcgaa acagttatgt ttgttaaaaa
     5701 aggcgataaa gttaaagtta tcactggaaa agacaaaaac aaagaaggcg ttgtactagc
     5761 agcgttcccg aaaaaagata aagttattgt cgaaggtgta aatgtcgtta aaaaacacca
     5821 aaaaccttct caagctgctc ctcaaggtgg aattgttgag atggaagcgc caattcatgt
     5881 ttctaacgtg atggtggttg actctactgg tgtagctggc cgagttggct acaaagaagt
     5941 agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa gttttagata aataaaagtt
     6001 taaggaagga gggtcttact gaatgaaccg cctaaaagaa aaatatctta aagaagtaac
     6061 tccatcattg atggaaaaat ttaactatag ctctgttatg caaacaccta aagttgaaaa
     6121 aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac gctaaaaact tagataaagc
     6181 agttgaagaa ttaactttga tcactggtca aaaaccaatg attacaaaag ctaaaaaatc
     6241 aatcgctgga ttccgtttac gtgaaggaat gccaattggt gcaaaagtta cattgcgcgg
     6301 agaaagaatg tacgaattct tagacaaatt ggtttctgtt tcactaccac gtgtacgtga
     6361 cttccatggt gtaagtaaaa aagcctttga cggtcgtgga aactatactt taggtattaa
     6421 agaacaattg atcttcccag aagtagatta tgatttagta gataaagtac gtggtatgga
     6481 catcgttatt gtaacaacag caaactctga tgaagaatct cgtgagttgt tgacacaatt
     6541 aggcatgcca ttccaaaaat aattaaagga ggcgaactac gtggctaaaa aatcaatgat
     6601 tgctaaaaac aaacgtcctg caaaattttc aacacaagct tatactcgtt gcgaacgttg
     6661 cggacgtcca cattcagttt atcgtaagtt ccatctttgc cgtatttgct tccgcgaact
     6721 tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc tggtaaaaag taaattcgca
     6781 taagaaggag gtaaaagatc aatggtcatg acagatccaa ttgcagattt tctaactcgt
     6841 atccgtaacg caaacatggt taaacatgag tctttagaag tacctgcatc aaaaattaaa
     6901 cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc gtgatgttga atacatcgaa
     6961 gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg gtaaaaacgg cgaacgtgtt
     7021 atcacaaact taaaacgtat ctctaaacca ggtttgcgtg cttatgtcaa agctgacgaa
     7081 gtaccaaaag tattgaacgg tttaggtatc gcaatcattt cgacttctga aggtgttgtc
     7141 actgataaag aagctcgtga aaaaaatatc ggcggcgaag ttatcgctta cgtatggtaa
     7201 tcaagtagaa tctacaagga ggtgtcttta agtgagccgt atcggaaata aagtcgttgt
     7261 tatccctgaa ggagtaacag ttactcaaga cggaaacaac gttacagtta aaggaccaaa
     7321 aggtgaattg actcgtactt tttcagcaga tatcaaaatg aatatcgaag gaaacgaagt
     7381 aactttcact cgtccaaacg acagtaaaga aatgaaaaca attcatggaa caactcgtgc
     7441 gaacttcaac aacatggttg taggtgtcag cgaaggtttc caaaaagcgt tagaactaat
     7501 cggggttggg taccgtgcac aattacaagg atcaaaactt gtattgaacg ttggttactc
     7561 acatccagta gaaatcacac caccagctgg agtaactgtt gaagtaccag ctaacacaca
     7621 agtaattgtt aaaggtgcga acaaagaaga agttggcgaa ttagctgcaa acattcgtgg
     7681 aactcgtcct ccagaacctt ataaaggtaa aggtattcgt tatgttggcg aatatgtacg
     7741 ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa attgcataga agccagcagc
     7801 aggaaaaagg catttggctg aagtttacct tcaaaaaaat acatttttac ttgtgccgca
     7861 gcttctattt ttcgaaacgt atttactgct tgaaatagta gtacaataaa aataaagagg
     7921 tgacaattgt gattacaaaa ccagataaga acaaaacacg tcaaaagaga catcgtcgtg
     7981 tacgtaacaa tatctctggt actgctgagc gcccacgctt gaacgttttt cgttctaaca
     8041 aaaacatcta cgctcaaatt attgatgacg tagcgggtgt aacgctagca agtgcctcta
     8101 ccttggataa agaaatttca ggtggaacaa aaacagaagc agcacaagct gtcggtaaac
     8161 tagttgctga acgtgcagct gaaaaaggca ttaaagtagt agtctttgac cgtggtggat
     8221 acctttacca tggccgtgtg caagctttag ctgaagctgc tcgcgaaaat ggactagaat
     8281 tttagaagaa ggaggaatac cattcatggt ttatatcgat ccaaaacatt tggaattaga
     8341 agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt aaaggtggac gtcgtctacg
     8401 ttttgctgct ttagttgttg tcggtgacaa aaacggacac gtagggtttg gtacaggtaa
     8461 agctcaagaa gttcctgaag caatccgtaa agcaattgaa gatgcgaaga aaaacttagt
     8521 tgaagtacct atggttggtt caacaatccc acacgaagta atcggtgctt ttagtggtgg
     8581 tagaatccta atgaaacctg ctgtagaagg ttctggggtt gctgctggtg gaccagttcg
     8641 tgccgtatta gaattagctg gggtagcaga tatcacttct aaatctttag gctcaaatac
     8701 accaatcaac gttgttcgcg caacagttga aggtttgaaa caattgaaac gtgcagaaga
     8761 agttgcagaa cttcgcggca aatcagttga agaattaatc ggataaggag gacaacaata
     8821 atggctgaat taaaagttac tttaaaacgc agtattatcg gacgtcctca aaaccaacgc
     8881 gatacagtta aagcgttagg tcttacaaag attaacagtt cagtggttaa gccttctaat
     8941 gaagcaatta aaggcatgat caatactgtt tctcacctag tggacgttga agaaatttaa
     9001 tttagaaaaa tcataactag aacattaagg aggtgccaaa cctatgaaac ttcatgaatt
     9061 aaaacctgct gaaggttcac gccaagtacg taaccgtgtt ggtcgtggta cttcatctgg
     9121 taacggaaaa actgccggac gcggacaaaa aggacaaaaa gctcgttcag gcggcggtgt
     9181 gcgtttagga tttgaagggg gacaaacacc attgttccgt cgtttaccaa aacgaggatt
     9241 cacaaacgtg aaccgtaaag actatgcagt cattaatctt gatgtattga accgctttga
     9301 agacggagct gaagtaactc ctgttgcttt agttgaagct ggaatcgtga aaaacgaaaa
     9361 agctggaatc aaagtattag ctaatgggga actaaacaag aaattaactg tgaaagcagc
     9421 aaaattctct aaagcagctc aagaagcaat tgaagcggct ggtggatcaa tcgaggtgat
     9481 ctaatgttta gactattaaa agatgctttt aaagtaaaag acattagatc gaaaatccta
     9541 tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac atattactgt accaggcgta
     9601 aatgctgctt cattgcaaaa cctttcttct ttgccatttt tcaacatgtt gaacttggta
     9661 agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg gggtttcacc atacattact
     9721 gcttctatcg tcattcagct tcttcaaatg gacatcgtgc ctcgatttgt tgaatggtcg
     9781 aaacaagggg aagttggtcg taaaaaactg aatcaagcga ctcgatatct gacattagta
     9841 ttggcattta tccaatctgt cggtatcact gctggattca atcaatacgc tcaattaggt
     9901 ttcgtgaata atcctaacgt agcaacttac gtaacgatcg gtttgattct aacagccggt
     9961 acgatgtttg ttacttggct aggagaacag atcacagaaa aaggtatcgg aaatggcgta
    10021 tcaatgatca tttttgcagg tatcatttct agattgcctg aaagtatcag agaaatttat
    10081 gaagattact ttataaatat tgatccttca gacatatgga aatctgctat ttttgtggcc
    10141 attttactag tagcggtctt gttgatcgtg acattcgtaa catttttcca acaagctgaa
    10201 cgcaagatcc cgatccaata tacgaaacgc gtagctggcg cgcctacaag cagctatctg
    10261 ccgttgaaag taaatgcagc tggagttatt ccagttatct ttgctagttc gtttattgct
    10321 acgccaaatg ctgttttaca agcattgagc ggtagattcg gttctgaaaa ctggtacaaa
    10381 gtgatgatgg agatctttaa ctataataca gcacctggtg ctacgattta tacgattctg
    10441 atcgttgcct tcactttctt ctacgccttt gttcaagtaa accctgagaa attagcggaa
    10501 aacttgcaaa agcaaggaag ttatatacca agcgtacgcc ctggtaaggg gactgaagag
    10561 tatgtctcaa gattactgat gcgtttaagt acagttggtg cagtcttcct tggtttagtg
    10621 gcattgcttc caatcattgc tcaaatggtt tgggatcttc cacaatcaat cggattaggc
    10681 ggtacaagct tactgatcgt tatcggtgta gctttagaaa ctgcgaaaca gttagaaggc
    10741 ttaatgctga aacgtaaata tgttggattc attgagtaag aaagcgtgtt gaggtttccc
    10801 ctcaacacac gttctcacac atttattagg agggaaaaca atgaacctca ttttaatggg
    10861 gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc attgatgctt atggtattcc
    10921 gcacatctca acaggagata tgttccgcgc agcgatgcaa aacgaaacag ctcttggcct
    10981 ggaagcaaag tcttatatgg ataaaggtgc attagttcct gatgaagtaa caaatggaat
    11041 cgtgaaagag cgactagctg aaccagatac agagaaaggc ttcttattag atggctttcc
    11101 acgtacgcta gatcaagcaa aagctctgga cgcaatgcta aaagatttga ataaaaaaat
    11161 tgatgctgtc atcgatatcc atgttggtga agaaatcttg gtcgaacgtc tagccggccg
    11221 atttatctgc agcaattgtg gagcaactta ccataaaatt ttcaacccta caaaagttga
    11281 agacacatgt gatcgttgcg gtgggcatga attctatcaa agagaagatg ataaacctga
    11341 gacggttaaa aatcgtctgg ctatcaacat caaaaatagt gaaccaatct tggcttatta
    11401 taaagaacaa ggtttgctaa acactattga tggtgctcgt gaaatcgatg ctgtatttgc
    11461 agatgtacaa aaaatcattg aaaaatagca cgattgcccg tagccgactt gcattatgca
    11521 gttactcttg tcaagttcaa taaattatga tagaatatgt cagtccgact tctagagaat
    11581 cctctagatg atccattttg agaattttga ggtgggaact gcagtttccg ccattttatc
    11641 gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt ggcaaaagaa gatatgattg
    11701 aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat gtttaaagtt gaactagaaa
    11761 acggacacca agttcttgct actgtttcag gtaaaattcg tatgcattac atccgtatct
    11821 tacctggtga caaagtaacg gttgaattgt ctccatacga tcttaaccgt ggccgtatta
    11881 cttatcgctt taaataattg tactccgtaa aactacagga ggtattatca tgaaagtaag
    11941 accatcagta aaaccaatgt gcgaacattg taaagtaatt cgtcgtaaag gacgcgttat
    12001 ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa ggataattgg aggtgctata
    12061 aatatggctc gtattgcagg agtagatatt cctcgtgata aacgtgtagt agtttcactt
    12121 acttatatct atggtattgg taacactact gctaaaaaga tcttagctga tgctggtgta
    12181 tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa cagatgctat ccgtgcagaa
    12241 gtagataaat taaaagttga aggggatctt cgtcgtgaag tgaacttgaa catcaaacgt
    12301 ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc gtggattgcc tgttcgtgga
    12361 caaaacacga aaaacaatgc acgtactcgt aaaggcccag ctcgcgctat cgctggcaag
    12421 aaaaaataat tcttaagtga aggaggttaa aacttcatgg cagcaaaaaa agtgaatcgt
    12481 aaacgccgtg tgaagaagaa tatagaagca ggtattgcac atatccattc tacattcaat
    12541 aacactatca tcatgatcac tgatactcac ggaaatgcat tagcgtggtc atcagctggt
    12601 tcattaggtt tcaaaggaag caaaaaatca actccttttg ccgctcaaat ggcagcagaa
    12661 actgctacaa aagcagcaat ggaacacgga ttaaagactg ttgaagtaac agttaaagga
    12721 cctggttctg gacgtgaagc agcaattcgt tcattacaag ctacaggttt agaagtgact
    12781 gcaattcgtg acgtgactcc agttcctcat aatggtgccc gccctccaaa acgccgtcgt
    12841 gtttaatgag tgccgctcat tttgagtttt atcagtaacg tcattgaaca agacactttt
    12901 cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa aaaccaagaa tcgcaaaaat
    12961 tgatgaagaa aaagattatg gcaagttcat cgttgaacct cttgaaagag ggtatgggac
    13021 tactttaggg aattccctac gtcgtatttt attatcttct ttgccaggtg ctgccatcac
    13081 taatattcaa atcgatggtg tactgcatga attctctacc atcaaaggtg ttagagaaga
    13141 cgtcactcaa atcatcttga acattaaagg cttagcgctg aaaatgtatg gccaagaaga
    13201 aaaaaccctt gaaatcgata ttaccggacc tgctacagta actgctggcg atattatcgt
    13261 tgatagtgat gtagaaattc ttaacaaaga tatgtacatc tgtagtgtct ctgaaggagc
    13321 tacgttccat gctcgcttaa cagtgaaacc tggacgtggt tatgttcaag cagatgaaaa
    13381 taaaaaagaa gatatgccaa ttggcgttct tccagtcgat tcaatctaca cacctgttcg
    13441 tcgtgtgaac tatcaagttg agaacacacg tgttggacac cgtgaagatt ttgataaatt
    13501 gacgatggag atttggacag atggttcaat tgaaccgttg gaagcaatga gtttgtctgc
    13561 gaaaatcatg actgaacatt tggatatttt tgtgaactta actgatgaag caaaaaatgc
    13621 tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa atgttggaaa tgacgatcga
    13681 agagttagac ttgtctgttc gctcttacaa ttgtttgaaa cgtgcaggaa tcaatactgt
    13741 acaagaactt acaaataaat ctgagcctga aatgatcaaa gtccgtaact taggacgtaa
    13801 atcattagaa gaagttaaag caaaattgca cgacttaggt ttaggtttac gtaaagaaga
    13861 ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat cgtaaactag gacgcacatc
    13921 tagccaacgt aaagcgatgt tgcgtgattt aacaactgat ttattaatca acgaacgtat
    13981 tgtgactact gaagctcgtg cgaaagaagt tcgctcaact gcagaaaaaa tgatcacttt
    14041 aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg gctttcgtac gtaatgaagt
    14101 tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa tcagctttac aaaaactgtt
    14161 caacgatatt gcacctcgtt acgcagaacg tcaaggcgga tacacacgca ttttgaagac
    14221 agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta gaacttgtct aaaatcacac
    14281 taataacatc actttataga tgattctttt agagtgttat gatgttggaa gatatttttc
    14341 cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt ggggagtaag cactcatcat
    14401 aaagtgtttt tttatacctt caattacctg tcatgacaga tatgtttgaa ggcataaaaa
    14461 aacattttaa gcgagaaata attgaaaatt ttgtcttttt ttgatagtgt tagtaggaac
    14521 tttaggatga agaaggagta atcatggagc caattattga attagaaaag ataaattata
    14581 aataccagcc agatgatctt cgccccgcat tgaaagatgt ctcctttaca attgataaag
    14641 gtgagtggat cgctattatt ggacacaatg gttcaggaaa atcgacactt gccaaaacga
    14701 tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt gggaaatcaa atattggatg
    14761 aagaaaatat ttggacgatc cgacagatgg ttggaatgat ttttcaaaat ccagacaatc
    14821 agtttgttgg ttcgacagta gaagatgacg ttgcttttgg tcttgaaaat caagggattc
    14881 ccagagaaga aatgctagtg cgtgtgaaag atgcacttga gaaagttaga atggctgagt
    14941 ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa acaacgtgtt gccattgccg
    15001 gcgtggttgc cttacgacct gatatcatta ttttagatga agcaaccagt atgctagatc
    15061 cagaaggtcg tgaagaagtg atctctacga tcaagaagat caaagaagaa agtcaattga
    15121 ccgttatatc tatcactcat gatattgatg aagcagctaa tgccaatcgg atactagtga
    15181 tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga aattttttct gctggtcctg
    15241 aactgatcga cttaggtcta gatctaccat ttcctgaaaa actgaagagc gcactgaaag
    15301 aacgtggggt tgatgtgccc agtgaatata tgacagaaga aaggatggtg gattggttat
    15361 ggacatccgt tttgaacaag tagacttcac ttatcagccg aatacaccat ttgaacaaag
    15421 agccttattt gacatcaata tgacgatcaa agaaaacagt tatactgcat tagtagggca
    15481 taccggaagc gggaaatcaa ccttacttca gcatttgaat gcgcttgtta agccaacaag
    15541 cggtacagtc catattggag aacgagatat tcagccggat acggataata agaatttaaa
    15601 gcccatccga aaaaaagttg gcatcgtctt tcagtttcca gaagcacagc tattcgaaga
    15661 aacggtagca aaagacattg ctttcggtcc taaaaacttt ggtgtcagcg aagaagaagc
    15721 actagtccta gcaaaagaaa cattagaaca agttgggctg gatgaaagct atttggaacg
    15781 ttcgccattt gaactttcag gagggcaaat gcgtagagta gcaatcgctg gtgtgcttgc
    15841 catgaggccg gaagtacttg tattggatga accaacggca ggacttgatc cccagggcag
    15901 aaaagaaatg atggagatgt tttggcgtct tcataaggaa cagcagatca ctattgtatt
    15961 agttacgcat ttaatggatg atgtagccaa ttttgcagat tatgtctatg tgttggaaaa
    16021 gggacgaatc gttaatagcg gggagccgca agaggtcttt caaaacattg aatggctaaa
    16081 agaaaaacaa ctaggcgtac ctactgccac cgagtttgca gaagaattaa tggctaaagg
    16141 gatgaacttt gctaccttgc ctttgacagc tgaagaatta gcagacgcta tcgtccatta
    16201 tgctggagga aatgcccatg atgaataaat tgatatttgg tcgttatatg cctggagatt
    16261 ccttgatcca tcgattggat ccccgagcta aattattagc cagtttttat tttatcggaa
    16321 tcattttttt agcaaataat tgggaaacgt accttttgtt ggcatttttc acattacttt
    16381 ctgtcttctt gtcaaaagta gatttgaaat tttttatccg aggcattcgc ccattgatct
    16441 ggctaatcct cttcactgta gctttgcagg tcttgtttac aagtggcgga gaaacttatt
    16501 ggagttgggg tgttttccat atcactgaat ttggtgtgat gaacggcctc tttattttct
    16561 gtcgattcgt gttgatcatt tttatgtcga ctctactcac gcttactaca ccgcctttgg
    16621 aattatcgga tgcgattgag tacttgcttc gtccattgaa agccgtacgg ttcccagtcc
    16681 acgaaatttc attgatgctg tcgattgctc ttcgttttgt gcctacattg atggacgaaa
    16741 cagaaaagat catgaacgct cagcgcgctc gcggtgttga ttttggtgaa ggaaacctag
    16801 ttcagaaaat gaaagcagtc attcctttgc tgattccttt atttgtcagc agcttcaacc
    16861 gtgcggaaga tcttgcgact gctatggaag caagaggcta caaaggtggg gaaggaagaa
    16921 cgaaatatcg tgtattgcat tggcataata gagacacgat ggttatcatt gcttttgtat
    16981 tattaactgt gctactgatc tttttaagag gataaacgtt cagtgggccg ctatctattc
    17041 ttttcttgct gatctgtttg caagaaagga tgcaagcagt gtatgacaag aaatattagg
    17101 gtctgtggca gactaaggtc acaggcctct ttcttttatc tgaaaagaaa gaacgaaaaa
    17161 attttataga aaaatcatta ttccagataa ccttttagga acaaaggaga gaaaaacatg
    17221 gtacgttaca aagcaatcat tgcgtacgac ggaacaaatt ttaatggatt ccaaaaacag
    17281 ccaaatgggc gtactgttca agaagaagta gaaaaaacat tacaaaaaat ggcaaatgga
    17341 aaagaaatca cggtctttgg ttcgggaagg acagatgccg gagtacatgc gatcgggcaa
    17401 gtaatccatt ttgattatcc agaggagcgt ccgttagaac gaatgcgttt cgcgctagat
    17461 acccagtcac cagaggatat tgctgtaaga caagtagaaa ttgtttcaga agacttccac
    17521 gcaagatatt tggtcaaaga aaagacgtat caatttcgag tggatatagg gaaaccacgc
    17581 agtccttttc gacgccatta tgcaagttat tttccttacc cacttgattt ggaaaaaatt
    17641 cagcgggcgc ttcctgattt attagggact cacgatttca cctctttttg tgcatcagga
    17701 agctcgatag aggataaaat acgtacgatc tacgaggcta agatggaggt aaatgaaaca
    17761 aaagacgaat tgttgtttac atttagggga aatggcttct tgtataagat gatacggatc
    17821 ttagtaggaa cgcttttgaa aattggcaat ggccgattgc ctgaagatag tatcccagat
    17881 attattgcga aaaaagatcg taatgcagca ggacctacag cacaccctga aggactttat
    17941 ttgtatgaag tgaaatatga ataagaggtt atgatagaag tggtcagctt caagaaataa
    18001 gaaggtattt acgaaaatta cttctcaaaa ttttgcaaaa tttcggctta ttttcgaaga
    18061 agctacttct ggaacaccgt ttaagaaaga ggtcgtgagc ctgtcattta actctgagtc
    18121 acaaagagca atttcaaaaa acagcttctc atctttgatg aatatcgtga cttgtggacg
    18181 aggcgttgat aagtgaatca tctttttgtt acctattttt tctgtaacca aaaaacgatg
    18241 gaacgttgtt ttgattgccc ttggtgcgga cagtctgaac aggttccatc gtttctttat
    18301 tttctacgtc ttcttctgtg tgtgtttcgt ggttgttctt ctattgctgg atcaatcgtg
    18361 ccataaaagg ggcgattgcg gtcaaggtac cagacataaa gaataaacaa tgcaatacat
    18421 aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca agtaactgaa agtgacagta
    18481 tgtttacctg ctggaacttt tatcatcaag aatgcgtctt tgaaattgct gaccttgact
    18541 ggtttcccat caattttagc tttccatcct ttgtcataag gaatagttgt caccaacatg
    18601 gaatcttcag cggtatttac agtcccagaa gcttttctgc cagtggcttc catttcgaca
    18661 cctttttctt tgatctgatt cattgcggct tgataggcta caacatctag accaacgact
    18721 ttaggctcca tgaaacttat ttctttcgtc ccataaaaac ttgcagtaaa tgtcactgtt
    18781 gtctcttgag gataatagcc aagatcatag tattgaccag aaatgttgat ttgcgttttt
    18841 cgcgatgtac cattgacagt gatcgtcgca ctagaacttt ctaattgcgc aaaattggtt
    18901 gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat ggacttccca agtgagctct
    18961 tttggctggt tatctgctgt ttctgagtaa gtgacgccag tcgttgtctt tgtgatcgtc
    19021 gtattcaact gattcttcaa agtaggtgta tagaatgtaa aatactgttc atttagacca
    19081 gacaatgtat tgatcaagtt tgtctgactg gtcaaattat ctgtcactgg ctgctcaaca
    19141 tcattaacgg acaacggtgc agtaaatgct agtggcaatg cattgacatt ctgatacagt
    19201 gtatagtcgc cgctttgacc agcttggcgg aagccatatt tactgaacgt gctttcgttt
    19261 ttagaaatgt tatatttgat tcctgtaaac ccatccatca gcaaagtatt attgttataa
    19321 cggatattca aatttgtccc tcttgaacgg aaacctaatt tatccaggta agaggaagaa
    19381 ttacgatttc ttatagaaga aaacaaactg ataccactgt aaccatagtt gatcgaatcg
    19441 tttgcagata ctggatccag attttccaag cggtaaaacg agtcggaatc tttttttgtt
    19501 tcatttacaa gagctttcaa gtcaggataa ggatcagtat ataaactgcg ggaagcataa
    19561 ttccaatcat caagaatgcc atggatcata ctgttcgtat tgaccagtgc ttcagcagac
    19621 atgatcaata ataacaatat actcagatga ttgaatgaaa gttttttatg ctgataaaat
    19681 cccacagcaa aagcataaat ccctaagaaa accactgtca atacaaagga agtcatcgtc
    19741 acataggtgt agcttccttc aggtttgatg ccccatgcca ccgcaaagag agcagctacg
    19801 accaaaatga ttcctaacaa cagcccacca tcgtcttgtt ctatcttttc ccatcctttt
    19861 cctgcaagca ggagcactaa gaaagagaac agataggcat aacggaataa aaacatattc
    19921 ggtgcatgca taccatgcca gaacaaattc aatggaacaa gataaaaact tgcgataagt
    19981 atggcgaaaa gactgccgta caaaaattta tttttcaacg gtactttccg actggcaaaa
    20041 taaaacagac agaggactaa tggtagcaga cctgcgtaaa cgaacggaat cgatccgtat
    20101 tttgtcgtgt cataaacacc aatcatattt ttcatgatta gatccagata agaagtcgct
    20161 tctgttttca acgtagtgat ctgactcagt gcctcgccat ttgaccgaag atccagcact
    20221 gctggcaata caatgatcat tgaagcgccg cctgccagta aagaagtgat accataagga
    20281 acaatcgttt ttttatattc tgaccagttt gtcaatagac gggcgataaa atataagaaa
    20341 gaaaatacgc caatcataaa acccatataa aaactggata aaaacaagag taaataactg
    20401 ataaataaaa gtttcggccg tttaagatcc ataacacgat gaatccccaa aatcactaat
    20461 ggaagataaa caaatgcatc cagccacatg atgatctccg aatgtgcagt gataaatgag
    20521 atcaatgcgt aactgacgct aagtgcaaca tgcccccagc gaggaatctt gaacgtacgt
    20581 gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa gcaaagtcag aacatacagt
    20641 gcatctggca tcatctggtt tggaaaaaag aacaccagtg gcgtgaagat cccgcctaga
    20701 tagtaagaaa ttaaagataa ataattcaat cctagagaag cattccatgt ataaaaaata
    20761 ctttgctttc caagaagaac atttcggaag ctggcatgaa aattagaaaa ctgtgaaaag
    20821 gcatcacttg ccaaaatact tctgctgctt ccaggataga ttccgatact tagataaaca
    20881 agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg cgtagcgtcc gttttctcta
    20941 aagaatgact ttaaattttt tttcatattt attttccacc aaatttctat gaagttgtat
    21001 tcagtttatc atatccttgt ttttctctgc ttaaaaagag tctagttttc acaaaaaaat
    21061 aaaatttaga ataaataact tcataatttg ttcgtataac aaaaataaac gcaaaa
//
LOCUS       DAZKCY010000039        21057 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000039 DAZKCY010000000
VERSION     DAZKCY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.039"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(265..1572)
                     /locus_tag="ACG8XC_001326"
     CDS             complement(265..1572)
                     /locus_tag="ACG8XC_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFP9191033.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(1575..2156)
                     /locus_tag="ACG8XC_001327"
     CDS             complement(1575..2156)
                     /locus_tag="ACG8XC_001327"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9191034.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(2272..2369)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2728..4020)
                     /locus_tag="ACG8XC_001328"
     CDS             complement(2728..4020)
                     /locus_tag="ACG8XC_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9191035.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(4017..4640)
                     /locus_tag="ACG8XC_001329"
     CDS             complement(4017..4640)
                     /locus_tag="ACG8XC_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191036.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(4643..5479)
                     /locus_tag="ACG8XC_001330"
     CDS             complement(4643..5479)
                     /locus_tag="ACG8XC_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9191037.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(5496..6383)
                     /locus_tag="ACG8XC_001331"
     CDS             complement(5496..6383)
                     /locus_tag="ACG8XC_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9191038.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(6399..7682)
                     /locus_tag="ACG8XC_001332"
     CDS             complement(6399..7682)
                     /locus_tag="ACG8XC_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9191039.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(8365..9048)
                     /locus_tag="ACG8XC_001333"
     CDS             complement(8365..9048)
                     /locus_tag="ACG8XC_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9191040.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(9066..9683)
                     /locus_tag="ACG8XC_001334"
     CDS             complement(9066..9683)
                     /locus_tag="ACG8XC_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP9191041.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(9693..10517)
                     /locus_tag="ACG8XC_001335"
     CDS             complement(9693..10517)
                     /locus_tag="ACG8XC_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFP9191042.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(10510..11070)
                     /locus_tag="ACG8XC_001336"
     CDS             complement(10510..11070)
                     /locus_tag="ACG8XC_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191043.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            11244..12200
                     /locus_tag="ACG8XC_001337"
     CDS             11244..12200
                     /locus_tag="ACG8XC_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9191044.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(12505..13461)
                     /locus_tag="ACG8XC_001338"
     CDS             complement(12505..13461)
                     /locus_tag="ACG8XC_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFP9191045.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            13565..14167
                     /locus_tag="ACG8XC_001339"
     CDS             13565..14167
                     /locus_tag="ACG8XC_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9191046.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(14255..15754)
                     /locus_tag="ACG8XC_001340"
     CDS             complement(14255..15754)
                     /locus_tag="ACG8XC_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP9191047.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(15770..16294)
                     /locus_tag="ACG8XC_001341"
     CDS             complement(15770..16294)
                     /locus_tag="ACG8XC_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP9191048.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            16592..17341
                     /locus_tag="ACG8XC_001342"
     CDS             16592..17341
                     /locus_tag="ACG8XC_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9191049.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            17332..18117
                     /locus_tag="ACG8XC_001343"
     CDS             17332..18117
                     /locus_tag="ACG8XC_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9191050.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            18086..18802
                     /locus_tag="ACG8XC_001344"
     CDS             18086..18802
                     /locus_tag="ACG8XC_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9191051.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(18904..19923)
                     /locus_tag="ACG8XC_001345"
     CDS             complement(18904..19923)
                     /locus_tag="ACG8XC_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFP9191052.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(19942..20943)
                     /locus_tag="ACG8XC_001346"
     CDS             complement(19942..20943)
                     /locus_tag="ACG8XC_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFP9191053.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         6323 a         4481 c         3666 g         6587 t
ORIGIN      
        1 ctttttatgg ataaacggtg ttctcctgcc aactcctcgg tcacaaggaa caatattcaa
       61 caaacatgag aagctttttt ttgaaattgt tccttgtgac tcggagatga acggcaggct
      121 cacgacctct tccttcaacg gtgttcaatc agctgaccct cggtattagc tcaaacaaac
      181 ggcaaaatac gaggaactat ttttccgttt gtttctctaa caatcaggtc aaaacgctga
      241 tttcacaacc tttcttttgc tctattactt cttgaaggct tcttccaata tttcttcatc
      301 ttttttcaat ccattaaaga tcaaattcaa tatgatagcc gtaatgctac tcattacgat
      361 gccatttcca gtgaacattt gcaatgtttg cggcatttga ctgaataatg tcggcgtaac
      421 attgaaaccc agcccaaatc caacagaaac agcaacaatc aatagatttt tatcattgct
      481 gaaatcaact tccaacagca tacggattcc ttggactgcc accatcccga acatgactaa
      541 cattcctcca ccaagtaccg gctcagggat gatttgtgct aacgcaccga ttttagggaa
      601 taatcctaaa acaatcaaga agaatgcaga gaaatagatg ggttttcttg ttttgattcc
      661 cgaaagttga accaatccga cattttgaga aaagccagta taagggaatg tattgaaaat
      721 accgcctaag ataacggcaa gtccttctgc acgatatccc tttttcaatt cttcttctcc
      781 aacatttttc ccagtaatgt caccaagtgc aaaatataca cctgttgatt ctaccatgct
      841 gaccatggaa atgatgatca ttagtaaaat cgaccatacg tcgaaagttg gtttaccaaa
      901 ataaaagggt tgtggaacat ggaacaacgg cgcatctcca atcgcgctca gatcaatcat
      961 tcccatgaaa gcagctaaga tgcttcctcc gactaaacca atcaataccg cgattgaacg
     1021 aatgaaccct tttgccagca tttgcacgcc tacaattagg agaatcgtca caaatgcaag
     1081 tagcagattt gttttgtcac caaaatcagc tgctgttgca ttacctccac ccattttctc
     1141 gattgctaca ggaatcaaag tcaaaccaat aacggtgata accgttcctg ttactatagg
     1201 agggaatagt tttttaattt tagaaaagac accagaaact aacacaacaa agatcccaga
     1261 agcgataata gatccgtaga tagctcctac tccttcattg gttccgatca agatcagtgg
     1321 agctactgct tggatcgcac atcctaaaac gacaggcaag ccaattccga aaaatcggtt
     1381 aactgtcaat tgcaataatg tcgctaatcc acacataaag atatcaattg aaatcaaata
     1441 agtcatctgt tcttgattaa agcctagtcc tgtaccgatc aaaagaggca ccgctactgc
     1501 tcctgcatac atcgctagca gatgctgcag ccctaaaacc gcagcttttc cattttgtgt
     1561 ttctttttcc acgattatgc gtcctcctca agaaactcaa cttgtccatt cgtcaaggaa
     1621 gcaatacgcg caagagaaac gactctcaat cccatttctt caagtaactg acgtccatct
     1681 tggaaagact tttcgatgac gatcccgatt ccttctactt gcgctccagc ttgctggcat
     1741 aattcgatca atccttttgc cgcttgtccg tttgccaaga aatcgtcgat aatcaatact
     1801 ttgtcttctg aagataaaaa tttacgagaa atagaaatcg tacttgtcac ttgtttcgta
     1861 aaagaataaa cagaagctgt caataattct tcatccatcg tcaaactttt tgcttttcta
     1921 gcgaagatca ttggaacatc taattctctt gcagcaaata aagctggtgc gatcccagaa
     1981 gcttcaatgg tcacaacttt cgtgattcct tgttctttaa atacttctgc aaaccgtttt
     2041 cccatttgtt ccatcaatac agggtctact tggtgagtga cgaagctgtc gacttttaat
     2101 acgccctcgc ctaatatacg tccgtctttt tgaatacgtt caactaattc tttcacttta
     2161 tttgctcctt tatcatttat taataccgaa ataaccgctt ttgtgcaaaa acctactatg
     2221 tttctgttaa agaaaaacac cctccacttt atcataagaa aagaagggtg aaatagccaa
     2281 tcttttactt atagtccagc atttacggta ctggctagaa actgtcatcc atattatgac
     2341 gatatataag taggtatgta aaactaataa tagtgtaaat tcattttctt gcaactgttt
     2401 tgcacgtgaa ggcagtttgc catatttgaa taaaaaatgt tgtttcaaat attgaaaaca
     2461 gtctaacact actttctgaa actgacaacg tggtttactt atttttttgt aaaatctaat
     2521 gatttttaat gcattattcc tttttgttcg ggtttaaata gttattttta ttttctccta
     2581 tctaaaaaag cagcccacat aaaaagaagg catgacaaaa agcatgtcat agcccctttt
     2641 tccgaataaa tagtgttcaa aagacagcct atcggcaata aggcaagatt ttataaacta
     2701 cgggaagtaa tttccgtaaa tctattctta ttcttttaag ctgacaagct cgtcaatcac
     2761 tactgctttt cctgaagcaa tagaatgatt tgctgcgatt cctgtcaaaa tcgacaaaat
     2821 accatctacg tgagttgctg cccgattcca cggatcttct tctttttctt caccaaatag
     2881 atcatctaat aaacgcggat ctcctccgcc atgtccgccc gcttcttgtt cgacgattac
     2941 ctcatatggc tcaccgaaca tcggatagac atggatggac tgaagaacgg ctgctccttc
     3001 atccttttta tctccacctg cattgatgta agacttttcg atcactttca tttcgatccg
     3061 tcctttgctg ccattgaaag aaacattgaa tccttcccac ggcatatacg cgtttagcga
     3121 atacgtcaaa atcgcaccag tttcatattt gaccgttacg cctagtgtat cttcaataga
     3181 gatatcatcg gaaaatacac tgcggtcacg aatatatcca ctgtcttttt ctgcttctaa
     3241 atacatttct cgcaattcct cattggctga caggtctata gcaaatggat cagctttagc
     3301 tagttcgctt ccatagcatc gactgtaaaa tatttctact ccgcgtttct ccgcattatg
     3361 catcccataa aagtttaatc caccgaatgc aaataccttt tggggacggc tgttcaacca
     3421 aaaattcact agatcaaaat gatgggtaga tttatgaaca agaagcccgc cgctattgtg
     3481 cttatttcga tgccatcttc tgaaataatc tgccccgtgt tgcgtattta acagccattc
     3541 gaagtggata gaaaacacat caccaatcac atcactttct atcagttcct ttatcttcgt
     3601 gtgatacggt gcataacgat aattaaaagt gactcgcagg cttcttcccg tctgtttttg
     3661 gaccgttatt atttcttgag ctttttcctc atcgatagtc agaggttttt cgcaaataac
     3721 atcatatcct tttttcattg cacgaatgat ataatcatga tgcgttcgat ctactgacgt
     3781 aataatgact tcagccggcc gtacgtcatc cagtagttgc tcaaattgtg atggcataca
     3841 tgtctttact tttttctgtc catacttgtt cacaagtatc cgatttgcat aatccatcct
     3901 tgtttgagat acatcacaaa aaccgactag ttcacaagtt tgatgatacg tagaggcaat
     3961 tgcctcataa aaaaaacgcg ctctcccacc aagtccgact tgtacatatt ttttcttcat
     4021 ttaaagtcat tctccttttc ttttaagcaa aatactgtga caaatggcag ccctggatac
     4081 agtaatgcag tgagtaaaaa atcatgctgt atacaccaga acagtccata caaaaggcat
     4141 aaataccaca gaaacccctt gacgtttttt gacaacagcc agatattcct tttgatcaaa
     4201 taaacaagac tatctgcttt atcttctttg cagatatcaa ttagcgtact aaaaaaacta
     4261 tgggatacaa aaagcaacag acacaataga aaaggaatca gataccaagc tccaatcttt
     4321 cccactaata aataccaatc aactgcaaaa ataatgacaa acagctgaaa caacgtaatg
     4381 atgaaacaca tccgtttgat ttttcctttc ctccaacata cctttttttc tgccaagtaa
     4441 tggacagata gataataaga aggatacatc atcaaaaaat aaatcagata gacaacacga
     4501 gcagacagca ctaatcctaa agtgaacaaa catcctagaa ctaaagcgaa aagagcatta
     4561 ctgataagac tggttaaaaa aatccgacag acatagtagt tgagcttgta aagaaacatc
     4621 tcttctattg gctttttcat attcagccct tcagaccgct tgtactgatc ccctggacaa
     4681 gatatttatt gaagaataag aaaatcagaa aaaccggtag cagcgacaac gtagacatcg
     4741 caaacatact cgcgtaagtc gtactagatc cagcgtctgc aaataatttg atggcaatcg
     4801 ataccgtgta agatctcggc ttgtttaaat aaatcaatgg tcccatgaag tcgtcccatt
     4861 tccaataaaa ctggatgatg atcgtcgtga tgatcgaagg tttcagcaaa ggaaaaataa
     4921 ttttggtaaa gacactatac ttgctgcatc cgtcaataat cgctgattca tctaactctt
     4981 tggggatgct gaccatgaat tgcatgatct ggtaaatgaa aaatgcttgt ccaaaaaaat
     5041 gtggtaaaat cagcggcaca tacgtcccaa ccaaattcag ccgattgtaa ataataaact
     5101 gcggaatcaa aatcacttgt gcaggaatca tcatcgttac taacatgacg acgaaccaga
     5161 atctttttcc tttgaaatcg attctcgata atgcgtaagc aatacaagtc gagctcagaa
     5221 ctgtccctat cgtcgaaact gcagagatga caaacgaatt cctaaagaaa gtaaaaaatg
     5281 aaattccagc aaaccccgcc catccttttg cgtaattttc ccattgaaaa gtctctggaa
     5341 tcaaattcag cgaaccactc ataatctctt gatccgtttt aaaagaacct gcgatcatcc
     5401 ataagaccgg ataaatcatg actagaccta atagcaaaat aaaaccgtat tgaaaccatt
     5461 tcgttactgt attattcatt tcttcctcct ctcattcaca caacgaccca tttttttgat
     5521 gatttgaaaa tcagaaaggt gacagctccc ataatcaaca gcatgatcca cgaaatagca
     5581 ctagcatacc ccatagaatt aaaagaaaaa gcttggttat acacataaag agcataaaag
     5641 ttcgtggcat cattcggacc accttgggta atgataaatg cctgcgtaaa agtcataaac
     5701 gccgcgattg tctgcataac caagttatac aaaatgatcg gcgaaagaag aggtaacgtg
     5761 atcttgtaaa acgtctgcca tttgttcgct ccatcgatct ctgccgcttc ataatacgag
     5821 cctgggatct ctttgatacc tgcagcaaag atgatcattg ccgaaccgaa ttgccaagca
     5881 gacatcaaaa caagagggat catgaccagt ttttgatcgc taaaccagga aagcgcaggt
     5941 gcgcctaatc gttccaggaa aatattcact aacccgtttt tagaaaacaa ctcccgccaa
     6001 actagagcga tagaaatact gccaccgatc aaactaggca cataatacaa agaacgaaag
     6061 aaagtcgttc ctttgatatt cctcgttaaa agataagcaa tcaatagacc aaagatcagt
     6121 ttcacaggta cagatgcaaa tacatagaga aaagtgactc ggatggagtt caaaaaacgg
     6181 gcatcactca gcatctttat ataattatcc aagccgatcc atgagactgg atttcccaaa
     6241 cgataatcgg tgaacgaata ataaagtgac aagatcatcg gtatcactgt tatcaataga
     6301 aatcctagga tgaatggaga agcaaatacg taaccgacca cattgtcttg atccaagaac
     6361 cgttttagtt tttctttttt catgccttcc tcctgctgct atcctagaat gtttgacgca
     6421 aactcataaa gactctctgc tgcttcttct gctgttactt cttcataaat gactttttca
     6481 actaaaagat tgtattggtc ttgaatttcc ggattcaaag cactatcaaa gacattcact
     6541 tcgccgttgt ccatatcgga aatcatttct aaataatcaa atgtcgcttg caaagttgca
     6601 tcatctgcac gagcgagctg ttctcgaata ttcgacatga ttggtacacc acgttctcct
     6661 tttaaaattt cattggcttc ttcactgttt tgaaagaaat caataaactt agcagcttct
     6721 tctttatggg ctgagctttt tgccatactc agcatttgcg aagaacgtaa agggatacca
     6781 gattctccat ctgcttttct gcgaggtgga ttaatcaact gaatctccat atcttcagga
     6841 gcccctgaca atatttctgt caactggtta cttgatagaa acaccattgc cgcttctcta
     6901 tccctaaccg cctgaaaaga cagatccgtc gtacttgcag ctccaccacc tggttctgga
     6961 tgggcccctt tgtctaccag atttttacgc agctggaaaa aatctgttag atagtctgga
     7021 ttttcaaatc ccaagctgtt agatagatct tcccgattat aaaaattcaa tcctttctca
     7081 acttgtggta tttgaataat acagccagct ataaaatctt cgaatcttga aatagcatag
     7141 attcctaatt cttcagtgat ttgatagctg atttgttcaa attcatccca tgtccaattt
     7201 gaagctggtt cagggatatt taattgttca aatatatttt tattgtaagc aatcgcaaac
     7261 gaattcaccc cgttagacag tccaactaat tgcccgtcgt aagtcgtcgt tgctaaatag
     7321 gattcatctg cattagaaat atcaattatc ccttgctcca catattcatc taatggttca
     7381 atctgattga tgtattgagg aaattgattg ttcagttgga aaatgtccca gatatcatct
     7441 gatgctgcta gtgtacctaa ttttgtgtaa tatccttcat aatcataaaa ttcataatcg
     7501 atcgtcacat ggggattttt ttcttcataa agctcgatta cttttactgt tgcatcgtgt
     7561 cggacttgcg aaccccacca agccatgttc agcgtgattt ttttgtcatc ttcttgccca
     7621 caagccgtca agaagaataa cataacactt aaagctaaga tgattctcat tcttttcttc
     7681 attctctgca cctctacttc cattgttatt tttcaatata ctgtattttt atacagaaca
     7741 tatttattaa acgatctttt tcaaggggaa taaaaaccaa gacttgcctt atcgttctgc
     7801 ctaggatgaa caactcaata ctttcaagaa aaattagata gtgctgattc ttctttcatc
     7861 gctaaaaaca cttcttgctt caatgatggg aggataatta cgcaaagaaa aaaggatatc
     7921 agacaactgc ccaatatcct tgacatagct aaataagcat gcttcttcgt gtgcgctctt
     7981 ccctaaaaaa cagaaaaata actagtaatt ctgtatgaaa ttaggtacaa tatacacgtc
     8041 gatgcacaag cattgactat ggcaacttga ttggtcagtt cccgctgact tttcaagccc
     8101 cgacaattta gctgctacta aattgtcggg tgcctttttt agtttttatt cgtttttcat
     8161 tctatagaaa gcgcttcaat aaatcaagac atttttttgt tagaatgcta ataataacaa
     8221 aaggttttaa ctcttcttat gtacattaca ggtaaaaaaa caaaaaaatt tctctttttt
     8281 ttgaattctg aacaaatagt actggagtac aaaagacgag gaagagccac tatctgctct
     8341 tcctcgtctt ttgattctca cctgttattc atgattcttt ttctcagttt cccgatattt
     8401 ttccatttta cgactaaaaa gctctccgct acttggtggc gtatatgtga tggcatttgc
     8461 tcctgcttga atggtctcag caatactttc ttccgttgga ccacctgtcg caatgatagg
     8521 aagttcagga tattttttac gaaaatggcg tactgcttct gcagtttctt tacctgcact
     8581 gatattgata atcgaaatac cagctgctaa cttttcatct atttctgtat atttcgacgt
     8641 taccgtactg ataactggaa tatcaactac tcgacaaact tcttttactg tctctaaagg
     8701 cgtaggaccg ttcaaaacga cccctaacga accttgcgct tctgcgaaca tgctcatgta
     8761 ggaagaacga gcaccttgtg tcaaaccacc acctacaccg gagaaaactg gaatgtcagc
     8821 tgcctcaata atgcttttag taatcgctgg atgaggtgta aaaggataca cagcaattac
     8881 tgcattcgca tttgtattac gtatgatcgc aatatccgta gtaaagatga tcgaacggat
     8941 caattttcca aatatcttga tcccgcttgc ttctaaaatg atttccggta ctcttacgat
     9001 atcttttctc aattccgtca tgatttctgg cgtaaattgt tctgccatca gccacttctc
     9061 ctttgctatc tccggtcata ctgattcaat tgatacgtct cgatgacctg gatgattttt
     9121 tgcgcataac cggggtctgt cgcataacct gcatcttgca atgcttgtgc ggcttcctga
     9181 tagttggtgg ccgtaatcac tttttcatac agcgcaggat tccaatctac tccttggacg
     9241 aacagttgtg tatggtcatc catggactgt tcccatgaat catacacttt gaagtctcct
     9301 tggatagtta tccattcttc gttaacaaat tcctttgttt ccaggcttac tttcttctga
     9361 tccccataag ccttgatccc gaaaagatta ttgtatttgc tagccaaggt actttttccc
     9421 caatttgatt ctaatatagc ttgtcctaaa atgatactag gaagcactcc ataaccatct
     9481 tgtagttctt gggcatgcgg aacaagccgt tcaataaatt gctgatggga ttgcgcttca
     9541 ttttcttgct gattatcagt aaaaggagaa gacagattct tcaaggaaaa aacaaaggct
     9601 aagagtatca ctgctacacc ggcaaccact gctggccaac ggatattctt gcgcttttgc
     9661 tttttccact tttttttcgc catgatcaat tcctactttt catttaattc attcagatat
     9721 tcttctagcg tcaattggtg tttggtgata tactcagcgt tttctttacc gacataacgt
     9781 aagtgccaag gttcatatgt gatcttagtt atatcttcgt gtccttttgg ataacgaacg
     9841 ataaatccat actgcgcagc attatctgcg atccatttag ctccaggctg atctccgtaa
     9901 cttgcatcca atacagtaga aggataactg ttgtaccagt tttgatcgac gacatcgact
     9961 gccagacctg tgtggtgttc gctgtatcct ggctccgtaa tcgtttcttt agtaatcttt
    10021 gtcgcctcct cttctgatac accttgacga ctcatcacat cttggacatt ttgttcgaaa
    10081 acttgttgtt gggatgatac agaccggtaa gctgagacca tcactaatgg aaatccagct
    10141 gcttcagcag ccttggcaaa tgcttcatat tctggagcaa tgcgtttgtc gatcaagtat
    10201 ccgttactta atgatgccaa ttgagtagac tcatcaattt ctttttctaa tttgtgatct
    10261 ggaccaacca aaactagctt ccaatcgtct gttttaacat ctggcaattg tttggacgta
    10321 cgatcttttg tttctttgtc ctttaccttg ctgctggttg tttgactgtt cttttcttta
    10381 ctagcagttg ttttggtgct tgttcgttcg actgcttgat tgccggcttg aacatgcgaa
    10441 tcatcatcgc taaaaataac atagcccatt gctgcgatca taatcacaac tgctccaaat
    10501 cctaaaaatt tattcacttg aaaaatcctt tcgttgttct tcacgttccc cattcgcctc
    10561 atgaacgtaa gtttcttcca gattttcatt cacccaatcg agaagttcga ttttttctcc
    10621 ttcgatctgt ttttgaacat ctgctggcag acgaaaattc aaaatatcat catagcccca
    10681 ttcatcatat tgaatactta ctttcaagtc aaggtatcta ccagcctgct gattattttt
    10741 caaagggatc agctcgactt ttgctgcttt gctgctaagc catttttgcg cgatgcgtgt
    10801 tttgatttgc ttgtactctg caacggcaac gtttcgtttt tcgggaaaaa taatcgtttg
    10861 acgcaaaatc ttttgcgtta gcatccattc ataatcagtg aaaagatttt ttactttctg
    10921 catcgcttca gatggcagat ttttctcgcc atctttccat ttttgccatt gttctggact
    10981 gatcagcaag tattcatcta aaaaattttt ttcagtttca aactgttcta ataatacact
    11041 cataataaga tcaatatata cttgatgcac gtttatcccc tccttttaac tattctactt
    11101 aagttttgat caaaaagcac ctcttaaatc gctgctttca ttttttttaa tgtttcaagt
    11161 cctctcctct ttattcttcg tgatttttgt tataataata gacagacaag aaactcaatc
    11221 caactatgaa ggagcgtttt cagatgaaaa aatatcaatg gggaatcgtt ggtcttggta
    11281 ccatcgcaca tgagtttgcc gaaagcttca atcaagagac gagcgaacta acagcagttg
    11341 cttccagaac ttcagaaaaa gcggaaaact tcgctcaccg ttacaatatt ccaaaagctt
    11401 acggaagcta tcaagaaatg ttagacgatg ctgaaatcga catcgtgtac attgctgtac
    11461 caaatagaca gcatatcgac catatcttgg cagcattgga agcaggcaaa cacgttctat
    11521 gtgaaaaagc catcacgatg aataaaaaag aactggctga cgctatgcgc ttagcagaag
    11581 aaaagaattt gatcttagca gaagcgatga caatcttcaa catgccactt tatcagcagt
    11641 tacgctcaat aatggataca ggaaaactgg gggctttaaa aatgatccaa gcacctttcg
    11701 gcagttacaa agaaccagac cctaaaaacc ggttcttcaa tccagaatta gctggaggag
    11761 ctctgttgga tatcggtacg tatgctgtat ctttcgctcg tttctttttg agctctcaac
    11821 ctgaagtagt tgcctctaca atggttcctt ttgaaacagg cgtggacgaa caatccgtca
    11881 ctatcttgcg taacaaagaa aatgaacttg ctgctgtcag cttgactttt caggcaaaaa
    11941 tgccaaaagt cggtatcgtt gcctttgaga acggttatgt gacgattgct gattacccgc
    12001 gtgctgatcg tgcggagatc ttatttacag atggtacaaa ggaattcatc gaaagcggga
    12061 atacatctga agcaatgaat tacgaaataa agaatatggt acacatgatt gaaggtgact
    12121 tgcccaatcg ctctctcttc ttgacaaaag atgtcatcga aatcttggat caaatgcaag
    12181 ttctatggaa aaatatgtaa ttttaaaagg ttgtgaaaaa gctgttctgc atcaagtaat
    12241 aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggctggact tattaccgca
    12301 gaggaaagct tttgaacacc gtttaagaaa gaggttgtga gcctaccgtt cagctccgag
    12361 tcacaaggag caatttcaaa aaacagcttc tcatgtttgt tgaatattgt gacttgtgac
    12421 cgaggagttg acaggagaac accgtttaaa gaagaggttg tgaaatcagc tttttgacct
    12481 gaatattagc tgaattcaca acctctattt ttggattttt tgttgggaga attctgcctt
    12541 gatttcaata aaacttttaa aagactgcaa aaactggaac aattctgctc gatcctcgaa
    12601 ttcagggcga ctagtcggca gaggcatttg ccgatacaat acataaactt cttcaatacg
    12661 tgttaaaatt tgtctgccat catttgactc tgaaaatgtt tctgcagtat aaatcaataa
    12721 accatagata tgttttccat aataagctgg ttgctggatt ctctctagat tttccagcat
    12781 gtctttaatc acattggcct gtgctcttct catagagaag taagtttcgt agtatagcgg
    12841 ctgtctgagc cagtagtttt cttgatgttc tctggcatct tcttggcttt cgcgaatgaa
    12901 tgtcagtaaa tgttcgcatt tgatctgcac atcttcttta ttttctgata aaagaaattc
    12961 tgccatttgt ctcaaaatat ttcgaaattc tttttcgatc tgtatttggt tttccttcag
    13021 ctgcttcgta cgatctggca tgtaaacatt tgcaagcaaa gcaaatacta atccaatcgc
    13081 cattaatccc gcttcattgc cgatcagctg ccaagacatg ttttcctcca ctaaataatg
    13141 agtgaccaaa accgaattta ctactatccc ttcggttaat ttgaagcgag aggcaagcgg
    13201 gatgaacagg aataaaaaaa gactgaagcc aaaaatagta tacccaacag caaaaaaact
    13261 gaacagtgct gttgccgtag ctaatataaa ggctgcgatt cgattcatgc ccgtacgcaa
    13321 tgtagagcgc ttggtatttc ctacacttaa taatgcaatg atccctgcag ctggccaata
    13381 caacagcgat aagctacccg ctgtgaccat cgaaaagaag acactgatca ctgttttgac
    13441 tgttcgcatt cctatacgca tttacttccc ccttttttat tttaattata cttgaataat
    13501 gaaaaactag ctatcagaca aaagtttagg tatgatagta aaaagaccaa aaaggagaat
    13561 ctctatgcaa tttggccgtt ttcgactagg tatgcgtacg atcaaaagtg ccttagctgt
    13621 ttttttatgt atcttgtttt ttcatttgac tcaacgagga atcccgatga ttgctgcatt
    13681 atctgctgtt ttttctttgc gtcaggactt gactacaacc gtttcttttg gaaagtctag
    13741 gatcatcggc aatacattag gtggtacatt agctatcatt tatttttttg tcaaaagcta
    13801 ttttcacaat gattttttgg tagaattgat cttgcttcct attctagtta ttattgtgat
    13861 cgtcgtgtca gatggtatta acaataattc cggtatcatt tccgcaatag ctacgttgtt
    13921 gctgattgcg ttgagcatcc ctcaaggcga atccgctttg tatgctatcc aacgtgtact
    13981 agatactttt atagggacat tcattgcaat cggtatcaat ttctttcttc gtcctcctga
    14041 aacagaaaag aaggaagaac tatcagaaga tttggtcgaa ctccaaaaga aagaacgatt
    14101 gctaagaaca aatttacaag agatagaagc taagatccaa aaacaaaaga aaaatagcga
    14161 ggactaattt tcttttgcaa taaaataaaa aaatggagag aagaacttta aaaagtcttc
    14221 ctccattttt gattgaacgg tgaagtgtac tactttattc ttccttcatc gagttttgtt
    14281 ctgcctcttt tggcgcttca tacatattta cttggctagt agtcccacgt gtcgacaaat
    14341 tctctacgat ttccttcaag tcaagtcctg ttgtcgcttt tagtgtttct tgagtagaag
    14401 aaagcaaatt tgtggcataa tttgtcactc ggttcgctcc actggtttct cctgaactgc
    14461 ttcctgtatc gacaacagag attttttcga tatttcctag cggctgtgct gcttcgcgca
    14521 tcaattgcgg tagcatatcg ataaccatac tcaagacagc tgcttcgcca tattccttga
    14581 aggcattggc gatcttttgt tttgcttcgg cttctgcttt tccttttgct agtgctgctt
    14641 cggcttctgc ttgtccagct aaacgtgtct tattggcttc cgcttctgcc agtgcttcca
    14701 cacggaatcg ttctgcttcg gcttcagtca cttctttgac tttttgagct tgtgcttctt
    14761 gttctttagc gtaccgatcg gcatctgctt ttttcttcac ttccgaatca tattgctttt
    14821 cacgacgtgt gatttctttt tcttccagtt cgatttgttt ttgacgttct acgattttaa
    14881 tctgcatttc ctgttcgatt acttgttgtt gtgctcgggc actttctaaa ttgtaggctt
    14941 ggtctgcttt tgctttagca acatcttgtt cttgtttgta ggttgctaat ttcagttctt
    15001 tttcttttaa tgattctgcg atctctgttt gtcgctgtaa ttcagctgct tgtgattctt
    15061 tttctgcttg agcttttttg atacgcgttt cttttagagc ttccgcttct gctatatctg
    15121 catctctttt gacttgggcg attcttggtt tccctaaaga gtcaagataa ccgtttttgt
    15181 ctctgacctc tttgatagtg aaagaaacga tgatcaaccc catttttgca aggtctacag
    15241 aagcgacttc ttgtacgctt tgactgaact tatcgcgatt ttgatagatt tcttctacag
    15301 tcatagaacc aagaatcgag cgcaagtgtc cttctaatac ttctcttgct tcattttcca
    15361 gttcttctct tgtcttgcct aaaaactgtt ctgctgctgt agcaatctct tctacagatg
    15421 aaccgatttt gatgatcgaa gtaccatcac acatcaccgg tacgccttgt tccgtataca
    15481 cttcaggggt ggagacgtcc aatttactcg ataatagact gatccgattc gaacgttgaa
    15541 aaactggcaa gacgaatgcg ccacctccac gcacaatttt gattttgtta ttgctttcat
    15601 caatgtgcac atttttgctt cctaaataac ttccactgat gatcaacgct tcatccggtt
    15661 ttgctgtctg gtacttcgct acaaagacga tcagcaacat cagtaaaatc aaagctgcga
    15721 ttgcgatcgc gataaaaaca actggtattt ccatttaaca cacgctcctt taagctttta
    15781 tcatgctttc ataaggcatg acataacaga ttctttcctt gatctcaatg atcaatactt
    15841 tcgttccccg ctcagctgtc tgttcttggg ggctgtaaaa acaagctgga cgactaatgg
    15901 aaccggtaac acttttcaac tgaatttctc ccatacctcg tacagggatc ggtgtgatca
    15961 caacagctac ttgtccttcc agagtttttt cagaagacga catcgttgat tcggcatgtt
    16021 tcatcggcat caatatatag aaattaagca aaaataccaa cactatcgat atgcctccac
    16081 tagcaataaa taaaaataaa gatgattgat ctgttaaacg ctcacctaga aaaccaaata
    16141 agcttgtgaa agttaaccaa gggataataa gcatcggatc aagcgggcca tcaaaatcga
    16201 atacatctcc aaaaataatc aataggactg ccaatcctgc acaaatcagc aatgtatacc
    16261 aataaatcgt ctcaagtgcg attcccccta tcatatacac gcctccttcc atttctacgt
    16321 ctatcatacc aatgtttgaa aattctgtat agcttctttt accgactcag tggtgttttt
    16381 tataggaagg ttgtgtttat cgttgacaat ttgttaaatt tttgttttca tgttaaaatt
    16441 ttcacaataa attctttcga ggtagcctat gtttcctttt tttaagaaaa aaccattaaa
    16501 gctccagaac acgatcatcc ttgtcgtttg tttctccaca ttcgtttctt tgattgtttc
    16561 tgccatattg atcaggaatt ttattctttc gatgaaccga tctagtgtgt cagataaaat
    16621 ctctagcatt gctaaaatcg ttgccaatga taaactcatc caaaaaaagc tgcagactgg
    16681 tgacccgaca catgcgattc aaggaataac aaatgaaatc atgattgcat cgaatttaga
    16741 ttttatcgtt gtcatggacc ctgaaggcat tcgatattct cacgcagacc caagttacat
    16801 cggtcttcct ttctcaagtc atgaagatgt gaaaaaagtg attggaggac aagaattttt
    16861 ctctgaaaaa gaaggaatgt tagggttcgg cttccgctat ttcgtcccta tttattatga
    16921 tcaagagatt attggtgcag tttgtgttgg cttaacaaac aacacgattg acaatcaagt
    16981 caaagatgga caaaaaatga ttgttttttc attgattatt ggacttagcg taggcatcat
    17041 aggcgccgtt tttcttgccc aaaaaatgaa aaaagtactg cttggacttg aaccacaaga
    17101 aatagcagaa cagttaagtg aaaaaacgat tatcgaaaat gaagtagatg aaggaatctt
    17161 agccatctct tctgataaaa ctatcctctt gatcaataca cctgcaaaaa acattttcta
    17221 ccaaacagag cagctattgg atttcgaagt aggaaaaaaa tcagtgataa tctatatgac
    17281 gttttgttta aagagacgat tgagcatcaa acgaccgttc ggactcaatc catgtattta
    17341 aatgggatag aaactatttc tagcgttaca cctcttttta gcaatgaccg ttttttagga
    17401 gcagtcgcaa ctttacgaaa tcgatcagaa gtaatgaaat taatggagga gcttgggggg
    17461 acgaaacaat atataaactc catgagagct caaacccatg aattcatgaa taaacttcat
    17521 gtcatttctg gtttgattga tttgaagaag tatgaagaag tcacccattt tatccagcag
    17581 ctaaatcaaa attataaaaa agagattggc caagtctcaa gtctgatcaa agtacctgca
    17641 attgccggtt ttttattagg caaaataaac gaagccaaag aacaatctgt tgaaatgctg
    17701 ttagaaagcg attcttttat ccctaaaatg gagatggatg actccatcca ttcacttttg
    17761 caagtgttag ggaacttact tgataatgca aaagaagccg tgctacaaaa acatgaaaga
    17821 aacggacagg tccggattca tttaaactat gacacagaag ctgaagtatt catcgtgacc
    17881 gtctgcgatg acggtgtcgg gatatcagaa gaaatctcag aaaaaatatt tacgctaggt
    17941 ttttcgacga agggaatcaa tcgtgggttt ggattgaatc tcatccattc aatcgtcaaa
    18001 cgccacggtg gatttttaga attttccaaa caaacaaatg gcggtacaat cgtctatcta
    18061 gaattaccaa aaaaggaaga tcaaaatgat gacaaatgtc ctgataatcg aagatgatcc
    18121 aatggttgca gaattgaatc gtacttttgt ggaaagcatc cctaatatga acgtaatcga
    18181 aagtttcaga aatgtagaag aaggaaaaaa atatttcaga caaaaaaacg cagatctgct
    18241 gctgcttgat gtatacatgc ctggcgaaag tggtatccat tttttatctt ggcttagaca
    18301 gcaaggaaat gatatcccag tgattttgat cactgctgca gatgatatcg aaaccataaa
    18361 gaatgtgatc cgctacggtg ccttagatta tttgatcaaa ccgttcactt tgcaacgttt
    18421 tcagctggcg atcgaaaagt atcttgcttt ctctcagaca atccaaacga ctgaaaaagc
    18481 cgatcagaaa ttgatcgatc aagtattcaa ccacaccact cccattcacg aaataccatc
    18541 attcatcgat ttgcctaaag gtctgtcaaa actgacttta aaaaaaataa gtgcatttat
    18601 tctaaaagaa gatactattt tttcaacaaa agaactggct caaaaaagct cactgtcacg
    18661 tatatccgtc aagaaatatt tgaatttttt agtagaacaa gatatattga acgaaagaat
    18721 caaatatttg gaaatcggcc ggccgctaac ctgttatgaa ttgacaaaga atgggcggaa
    18781 aaagctcaca gattactgtt gagtcatcga attatcccat acaaaatagt ccaagtatac
    18841 agaagtaaca actgtttact tggactattt ttaaacattt tattgacgaa tatccatgtt
    18901 ttcttatctt aattgacggg ctttaggaag agatacctca tttttcgttt ttccagtttc
    18961 tattatttca cgaaagttct cgaaacttgt cgaaatcata tttgataatg cctcatctgt
    19021 atttgaacct acatgcgggg ttacaagaac tctagggtac atttctacta attgctggat
    19081 agcagggtct ggaatcgtct cccatggctt gaaggatcgg aaaaaaacct ctgtttcatt
    19141 tgcaaaaaca tcggttgcaa aaccagctaa atgattgctt ttcaaagctc ttaaaatagc
    19201 ttcattatct tgaagctctc cacgggctgt attaatcaag atgctacctt ttttcatttg
    19261 agctaaaaat ccttcattga tcatttgatc gttttcacct gggatatgag gtacatgaat
    19321 actgacaata tcgcttttgg ctaataactc ttcaagcgag tggaacgtca atactttttt
    19381 agcagcttct gtctgatgaa tatcgtagcc gatcactttg gctcctagtc ccttaaacaa
    19441 aacagcttcc gtcaacccta tcctgcctgt tcctatgatc cctactgtgc aatttctgat
    19501 ttcacgacta aacatttgct catccactcg aaaatctttg aaagatgttc ggatcgtcgt
    19561 atacgcagta tttctcaaaa gcatcatggc aaaagtcaaa gataactctg ctatcgcatt
    19621 tggcgaatac gatggtacac gggctactgt cataccaaaa tcagctgccg cttgtagatc
    19681 gatatgattg actcccacag tccgtgtaaa aacataacga atcccatatt cctgcatttt
    19741 tgcaagattg gtctcatctg ctatacaatt tcccctcaac aatacagcat catggtcctt
    19801 tgctgacacg atattgtcat gtgtcaacag ttcttcataa agactgagat cataatggta
    19861 aatattcaat ttttcaaaaa aatctatctc gttcggacga acaccgtaac atgcgattct
    19921 catactttct tcctccattt cttatgaaaa taaacctatg ccatgaatca tttgtaatac
    19981 aacgatccat acaggtgtca gtaccacagc aaaaatagtt gacagtaagg atgcattgga
    20041 ggctaaaagt gcctccctat caaaactgat agcataggct gctgccactg ttgcagctgg
    20101 tgtagccatc atgataacga ttgttgccag tgcttcaaaa ttgactggta gaatattcgt
    20161 tgccgtcaaa actgctagaa tcacgatatt caatgcaggt acaaatacca ctttgactag
    20221 actgtaatac catgaagttt tttcagatgc agcttttttg aagcttactt cacctaatgt
    20281 tgcgccgatt gctagccacg ccaatggtga acttaaacca gctaaataat ccatcggctg
    20341 aaatagccac ggtgcagtct ggtcaattcg caaaaacgct acttggctta tctctccttc
    20401 tgcacctgta actgacactt gtggcagata cccttggaac agccaaatga aaaggccggc
    20461 aaaagtagcg ataatgattg gattgacgaa catcgctttg atatttttag cttccatctt
    20521 caagccactc atttttatgt atccataaga atacaaaaag attcggtagc caatattgaa
    20581 aattgaggaa tacatcacgc cgacaggacc ataaatcgca ctgacgatag gcgttccgaa
    20641 aaaggtcgta gaaccaaaga tcgtcaggac acgcaacgtt gtttgcttat cccctttata
    20701 gcttaagaaa aaaggttttg aaactagaat aagaataata taaataacta atccccaaat
    20761 caagacattg attccttgca tcagtaattc tgtattgata tcttgcatga aagctcgaaa
    20821 tgccagtgct ggcaaagcca ctgttaagac gactttcgat agaatttttc caacagattc
    20881 agaaaaaatc cccttttttc tacaaacaaa ccctaataat ataataaaaa ctgttgaagt
    20941 gattgcactg acaatcgaca tatctgtcaa agtcgatcga aaaatgtcaa ataagttcat
    21001 ctttctcctc ctaattatgt tcatttattc acaataaaaa aagcttttat tttgcta
//
LOCUS       DAZKCY010000040        21054 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000040 DAZKCY010000000
VERSION     DAZKCY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.040"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            242..406
                     /locus_tag="ACG8XC_001347"
     CDS             242..406
                     /locus_tag="ACG8XC_001347"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191054.1"
                     /translation="MPATLNAYDGYDYRKKIVKKLYGATYGNVVSVLSVHHQASVTVA
                     EVEDAAVNGE"
     gene            602..769
                     /locus_tag="ACG8XC_001348"
     CDS             602..769
                     /locus_tag="ACG8XC_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP9191055.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            795..1139
                     /locus_tag="ACG8XC_001349"
     CDS             795..1139
                     /locus_tag="ACG8XC_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP9191056.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1144..1425
                     /locus_tag="ACG8XC_001350"
     CDS             1144..1425
                     /locus_tag="ACG8XC_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191057.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1528..1842
                     /locus_tag="ACG8XC_001351"
     CDS             1528..1842
                     /locus_tag="ACG8XC_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFP9191058.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1820..3514
                     /locus_tag="ACG8XC_001352"
     CDS             1820..3514
                     /locus_tag="ACG8XC_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP9191059.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3534..4712
                     /locus_tag="ACG8XC_001353"
     CDS             3534..4712
                     /locus_tag="ACG8XC_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9191060.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4675..5361
                     /locus_tag="ACG8XC_001354"
     CDS             4675..5361
                     /locus_tag="ACG8XC_001354"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFP9191061.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5361..6521
                     /locus_tag="ACG8XC_001355"
     CDS             5361..6521
                     /locus_tag="ACG8XC_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9191062.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6531..7406
                     /locus_tag="ACG8XC_001356"
     CDS             6531..7406
                     /locus_tag="ACG8XC_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191063.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7403..7714
                     /locus_tag="ACG8XC_001357"
     CDS             7403..7714
                     /locus_tag="ACG8XC_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191064.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7704..8057
                     /locus_tag="ACG8XC_001358"
     CDS             7704..8057
                     /locus_tag="ACG8XC_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9191065.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            8047..8448
                     /locus_tag="ACG8XC_001359"
     CDS             8047..8448
                     /locus_tag="ACG8XC_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191066.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8441..8845
                     /locus_tag="ACG8XC_001360"
     CDS             8441..8845
                     /locus_tag="ACG8XC_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191067.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8857..9465
                     /locus_tag="ACG8XC_001361"
     CDS             8857..9465
                     /locus_tag="ACG8XC_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9191068.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9485..9847
                     /locus_tag="ACG8XC_001362"
     CDS             9485..9847
                     /locus_tag="ACG8XC_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191069.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9850..10032
                     /locus_tag="ACG8XC_001363"
     CDS             9850..10032
                     /locus_tag="ACG8XC_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191070.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            10049..13480
                     /locus_tag="ACG8XC_001364"
     CDS             10049..13480
                     /locus_tag="ACG8XC_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9191071.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            13531..14268
                     /locus_tag="ACG8XC_001365"
     CDS             13531..14268
                     /locus_tag="ACG8XC_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9191072.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14278..16569
                     /locus_tag="ACG8XC_001366"
     CDS             14278..16569
                     /locus_tag="ACG8XC_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFP9191073.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16593..18719
                     /locus_tag="ACG8XC_001367"
     CDS             16593..18719
                     /locus_tag="ACG8XC_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191074.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            18736..18885
                     /locus_tag="ACG8XC_001368"
     CDS             18736..18885
                     /locus_tag="ACG8XC_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191075.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            18882..19328
                     /locus_tag="ACG8XC_001369"
     CDS             18882..19328
                     /locus_tag="ACG8XC_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191076.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19330..19467
                     /locus_tag="ACG8XC_001370"
     CDS             19330..19467
                     /locus_tag="ACG8XC_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFP9191077.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            19505..19798
                     /locus_tag="ACG8XC_001371"
     CDS             19505..19798
                     /locus_tag="ACG8XC_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191078.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            19795..20019
                     /locus_tag="ACG8XC_001372"
     CDS             19795..20019
                     /locus_tag="ACG8XC_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFP9191079.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            20016..21041
                     /locus_tag="ACG8XC_001373"
     CDS             20016..21041
                     /locus_tag="ACG8XC_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFP9191080.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT         7414 a         3185 c         4442 g         6013 t
ORIGIN      
        1 tgctgtctat tgttttttaa ttattcacac gataactaaa ggtgggtgaa gagaaatgat
       61 tccattaata atttcaattt ttgcgctctg tcttaatgtc tatatgattg gatttaaaaa
      121 tgggcaaaat aaaaaatagt agcagccaag aataatttta tagtgtcact gtggcggaaa
      181 gggtagacgc taagcatgtg tgctaggtca atgcttcggc aaccatgcaa ggttcgattc
      241 cttgccagcg acattaaatg cctatgacgg ttacgactac cgaaaaaaga tcgttaagaa
      301 gctatacggt gctacgtacg gcaatgtagt aagtgtgcta tctgtacacc accaagcttc
      361 ggtcactgtg gcggaagtag aagacgcagc ggtaaatggc gagtagcctc gtgagagcct
      421 ggtaagttct cgtgagtggt gcaatcccac tccagcgact ttaagcaacc gaggcatcgg
      481 cggtttaaaa atataggggt gcgcaatttc gtacgcgttt tgtgcatcgt gcaagttact
      541 attacatatt agatcactct ttgagtggtc tttttatttt tgcacaaagg aggaaacaac
      601 aatgtataga ccacaatact tagaacagaa gtatgaagta atcactgttc ataaaggtaa
      661 tggcgaaaaa gtatatgagt atagaagacc aataaagagc gatacatata aacgaaagga
      721 aaacaatgaa gttattccat tgtatggcaa aagaatagct aagcattaaa taagattgcg
      781 aaaggagacg gaacatgacc gaggaattct atagatggct attacagttg acaagagaag
      841 atcgtttggt taagttctat cagtctccta aatggcgcag gcttagagag aaagcgatga
      901 aacgagatca ctatgaatgc caagagtgta gaagactagg taagtatcat agagtagaga
      961 acgttcatca tataaaggaa gtcaaggata gacctgactt agctttagat ttagataatc
     1021 ttatttgttt atgtgttgaa catcataatg aagttcatgg cagatatctt acagcattag
     1081 ataaacaaga gaagaagata gaaagctttg ctaacttcga tgcaagtgaa aggtggtaag
     1141 tgcatgatca tcaatgataa tggcagagag tatgatacag aaaagattga agagtattca
     1201 tcttatactc agggattaat taaacgtttg atatacgttc gctatgtagg tattagggat
     1261 ctgttatcag ataactgttg tagtaaatac aaagtgaatc aagtaagaga agcgttgaat
     1321 aaagataata acgtcgaaag aataaaaaat gtttttggat atagtattga agagattaat
     1381 tattacattg acttcgctga agctttcatt ccgatggtga gataaccccc ccttaaaata
     1441 aatcgcaaat tttttggggg tgatgaaacg gagggggctg tcaggaaaag agattttttc
     1501 gaactttatc atgaaaggag ggctaaaatg tttaaaaacg aattgtctca aaatcgctac
     1561 agagaaaaat tacgccgctc tttaataagc caattggaaa gtcagaaaac aaatattgag
     1621 ccattcttag ataatgttga tcgttatatc agtttatggg aaacggcgat atcactggaa
     1681 gaagatatat ccgagaacgg cattagactg gagaatggta aaaagaatga atcagtagcg
     1741 ttgcttgttt ctgtcaacaa acaaatggga ttgatgttgg ataaacttgc cattactcct
     1801 gaattggtag gtgaagcaaa tgaatcaatt cctgagttat aagcatattg aaaattggtt
     1861 caaagctata gaagaaggca ctatcaaggt atgcaaagag caattattgc taaaaaatta
     1921 tctagaagaa agagtcttta ctagagaaga tatttacttc gataagcaga tggtagagga
     1981 ttcaatcaat ataccagcac aatactttcc attcgaatta attccgtggg aaaaatttct
     2041 acaatgtttt atttatggtg ttcgatggaa aaaagataaa acactagtgt tcaatagata
     2101 tctttcatta atgggacgtg gtaatggtaa aactggtttt gcttcttgga acaacttctt
     2161 tctactaacc gctaaacacg gtattaaaaa ttatgatatt gatatctatg ccaataatga
     2221 aagccaagca aagactagtt ttgatgatgt atttaaagta attaaagatc atcctgattt
     2281 agataaaaaa gtatttaaag ctacgaagga agttattcaa aatatcgcta caaacagcaa
     2341 acttcgttat aacacggcaa atgctagaac aaaagatggg aagcgaccag gtgcaaaccg
     2401 ctttgatgaa attcacgaaa atgaagatta ttcaatgata aatgtggcta cttctggtgg
     2461 tggtaaaatt cgagattata gagaatttta tgatacaact aatggtcatg ttcgtggtgg
     2521 tccgcttgat gacattatag aagaatcaaa aatgattctt tctggagaac ttggaattga
     2581 caaggatgga gcagaatttt ctagtttgtt tccatttatt tgtcgcttgg ataacgataa
     2641 tgaagttgat gatcccgaca tgtgggaaaa agcttgtcca actattaatt acaatgcaga
     2701 tttaaaacgg aaaatgtttc aagaatactc tcaaatgcaa cgtaatgctg gtttaagact
     2761 tacgttcatg accaaacgaa tgaacagacc tatggaagat acacgatttg ctgttgcttc
     2821 atatgatgat gttctgcata cgaaagaaaa agaatttcct gaaaaaatgg atgaagtgat
     2881 aggaacagtc gattttgctg atagacgaga ttttgccagc gttgggttgc taggaaaata
     2941 cgataaagat gtttatttta cacaacatac ttttatccac gaatcagccc ttcgattaca
     3001 aaacatcaaa cgagaggtta tagatatttc tatagatcaa ggaaaatcac agatcgttca
     3061 tggaaaaaat atagaagctg attatattgt aggttggttt cttgaaatga gtaataaata
     3121 ttatattaaa aaaatcgcta tggatatgta ccgtgcaaaa atattgaagc ccgctttaga
     3181 agaagcaggt tttactgtgg aaattgttcg aagcggatct gttacacatg gtatgttaaa
     3241 agatctggtt gatgaccttt ttattaatca acgtttattt tttggtgacg atgcgattat
     3301 gcgttggtat tgcatgaatg tatatgaaga gcatatttct aatggaaata tacgctatga
     3361 aaaaatagaa cctgaaacta gaaaaacgga tggctttttt tcattccttc atggtttgaa
     3421 ttttttagat gatatttatg attctgctcc tgtaacagtc acaaatagct cagtagaaaa
     3481 tacaggaact ggatttactc ctctagtatt ctaacttgaa aggaggtgag aaagtgggga
     3541 tttttcaaaa ggcggtagga tacttcacaa aaaaagcaac ggttccttta gaagaatact
     3601 tttgtaaatt gcaagttgat tttgtgtatc gaaaatttgc gattgaaact tgtattgatt
     3661 tgattgcaaa tgcgatgagt aaagcggaat tcaagtcata tgaagatgga aaaaataaaa
     3721 agaatgatct ttactatagg ctgaatgtag ctcctaataa gaaaaataat gcaacagaat
     3781 ttagaaaaaa actgatcagg agattaatat tctacaatga agtattgatc gtttctccgt
     3841 ctaataattc tagcgaaata tttattgcgg atagttggga tgtcacagaa tatgcattga
     3901 aagatgatgt gttttctcaa gtgcaaatta acaacatagt ccttgataga gaatttctag
     3961 aaagtgatgt tatctatata aaatacgcag atcaacaaat taggcaacta gtcgatgcgt
     4021 attatcaagc gtatgggaaa ctcatttcta gtgccatgaa tgtttacaag cgctctaacg
     4081 ctcgtagata cgtactgaaa gggaatttat tccgaccgca agacaataca acacaagatc
     4141 aaatcaataa aatgatgaca tcacaattta aggcttttat ggaagctgat aatgcaggtg
     4201 cggtatttca attacaaaat gagtacacat tagaagattt cagcggaaac ttccaaagca
     4261 attcaagaga tataaaaaac ttaatagacg acatctttga gatgacagca gcagcgtttc
     4321 acgttccgaa aaacctacta aagggagaca tgagtgggtt atcggatcaa gtggacgctt
     4381 ttttaatgtt cgaaatcata ccgattgctg aacttattca ggatgcgttt aacgctagtc
     4441 tctatgaagt agaagaatac ttgtcaggga attttgtacg tgtggataca actatgatca
     4501 agattactag cttcaaagat ttggttgacg ctattgatgt aggcattaga aatggagtat
     4561 ttacaatcaa cgaaggaaga gaacgcgttg gaaatgatcg ctctgataag gcgatggcag
     4621 atgaaatatt tataactaaa aacaatcaac aagtatcgaa aggaggtgag gcgaatgacg
     4681 acaatgaaaa catttctagc agtaaagaat gaaggcgcag taccgcaaat ttttattcag
     4741 ggatttattg gttctagttg gttctttgaa gggaatactg acaagggaat caaaaatatt
     4801 ttggatagtc taggtgatca agaagaaatt gaagtagtaa ttaattcaaa cggtggagac
     4861 gtatttcaag ggattgctat tgggaactta cttaagtcaa ataaagcaaa agttaacgtt
     4921 gtgattaacg gcttagccgc tagtgctgct tcaattatcg caatggctgg cgatactata
     4981 aaaatctaca acaatgcaca attgatgatt caccgcgctt ccacatacgg agaaggaaat
     5041 gtcgatgact tccgtacgat tgctgaccaa ctggaatcaa ttgataaatc ggtaaaggct
     5101 tcatataaaa cacgattcaa tggcacagat gaagcattgc aagaacttct tgaaaaagaa
     5161 tcgtttatgg atgcagaaac agctttgagt tatggattgg tcgatgaaat tatcgatgca
     5221 gaaaatagct caggtactga agctaaaaaa gaacaaagcg ttgaagaaat tttgaatgac
     5281 gttaaagaaa aaagagcaga aaaaattgct gcatttacag cagcattaaa taaaacattt
     5341 ggacaaggag atgtaaaata atgacagtta aaaatttaaa aggtgtaaca gctgcaagcg
     5401 accaattgat gaaagctttt aaagatggta acgaagaatc ttttagcgca gctatggtaa
     5461 gcttatctaa ggaaattcag gataaaattt tagaagaagc aacagcaaaa aatcaagatc
     5521 aattagtatt aatgaaccgt ggtcagcgtg tattaactac acaagaaaca aaattctata
     5581 acgaagtagt gaaaaacgaa ggttttgcag gggtcgaaga attagtgcca gctactgtat
     5641 ttgaacgtgt atttgaagat ttagaacaat ctcatccact attgcaaaaa attacttttg
     5701 ttaacacaac tggtgtaaca gaatggattg tgtcacgtgg agtcaatcca gcatggtggg
     5761 gtaaactttg cgaagctgtt aaaaaagttt tagataatgg ctttgatgta attaacatga
     5821 agcagttcaa gctatcaggt tatattcctg tatgtaaggc aatgcttgat ttaggtccag
     5881 tatggttaga tcgttatgtc cgtactgttt tagtagaatc attgagaatt gcattagaac
     5941 aagcaattgt tgatggtact ggtaaagata tgccagtcgg aatgatgcgt gacatgagca
     6001 aacaaactag cggagaatat gctgaaaaaa aagcagaacc tattacagct ttagatgctg
     6061 taactatggg cggtttgatg gcgcgactat caaaattcaa tatcgaaggt gtgaatgatc
     6121 cgatttatcg taatgtgaat ccttctgatg tggtcctaat tgtgaatcca actgattact
     6181 ggtctaaagt attcccagct aagactgtac taactgctaa tggagaatac gtacaagtat
     6241 tgccagtacc agtttcagat ttgcagtcaa cggctgtgcc agaaggaaaa gcagttattg
     6301 gggtagcctc agattacttc atgggtgtag gatctacact aaaaattgaa gcttcagatg
     6361 aataccattt tgttgaagac gaacgcattt atctagctaa acaatatgca aacggtcaac
     6421 ctaaacgtaa cgatagtttc attgtattag atattagcgc tttgggaact actactacaa
     6481 ctacaaaacc aacaaccaca acaactacaa cacaagcgta ggtgatcaga atgaagtata
     6541 ttctttgtca gccggcaatc aatcggttta aatgggagct tgaagtttgt ttaactaatc
     6601 tgaagaaact aggaatcaaa gatatcgtat tgcttttcag cagacacgat gatcagattc
     6661 ctattttttt tgagaaggaa tatggtgttg aagttcatgt gtacgatgat ctgcgggacg
     6721 acaaagagta tattccttcg attaaaccat atttatggtg gaaatattta gaagaagatc
     6781 attcgcgtga ggacgaccga tatttctata tcgattcgga tgtcattttc aataaaagaa
     6841 ttaatttgcg caaattgcct tctaaagatg atgtttggta ttgtagcgac tgctgtagtt
     6901 atctaagtct tgattatatt agaagctgtg aaaacggaga aaatattcta aaagatatgg
     6961 caaacattgt aaatgttaca gtagaatctt tggaaactat aaacactaat tcaggaggcg
     7021 cacagtgggt tattaaccgt cctaaagcga attattggaa aaaggtttat ctggattcta
     7081 atcggctata tcgctacctt agagggcaaa aaacaaatat acaaatctgg acagccgaga
     7141 tgtgggcaca gctttggaac atgatgtatt tcaatattgg tcctaaagtt cacgaggaat
     7201 tagacttttg ttttgctact gatccaatag aaaaagttaa agaagtaaaa atcttacaca
     7261 atgctggagt aacaacaaac gatgaagatt tatttttcaa agggagatac gtgacttcta
     7321 cgccttttga tgaagattta tcatttgtaa acaagaaaaa atgctcttac gcatatgtta
     7381 aagcaattaa ggcggtggtt agatgacgcc tgaacaagtg actgaagaat tgctaatagc
     7441 tgtgaaggat aatatttacg ttacctggaa cgaagaagat gagtcaatta aaaagatgat
     7501 agctaaaaat gctgtttatc ttcaaagtaa agtgagtaca acactttctt tttctcctga
     7561 aagcttagaa tacggattgc taatcgaaag atgtagatac gactggaatc gtgctttaga
     7621 tgagtttgaa caaaatttcg ctagtgagtt attaggtttc attcaacatt atgcgctaca
     7681 agaatatatt gcaggtgatg tgaatggcga ataatcgtag actcgaagaa acattcaacg
     7741 atggttggtt aaagattttg acgcaaacta ccaaaagaaa tgaactagga aaaaagattg
     7801 gtgtagaaga tacagaaatc acttctttaa aatttagaaa tctttccatg agagatagtg
     7861 atataacagc tatggatgcg atgggatcga aattaactaa gaaagtaaag actccatttc
     7921 atccaatcgc caagaaattt aataaagatc aatattttat cgtaatcgat agtatgcgtt
     7981 acaacgttat ctatgccgat tacgataatt tttatatcta tttttatctt gaaagtgtgg
     8041 gtgaatatgg tgattgataa ttctaaagaa aaagaacgtt taaataagca aatttctgct
     8101 atcaaaactt ccttagaaga gcattttggc ctcaaactct ttcaagactc cgtaggcgag
     8161 gatgagctac ctgatgattt taattacttc attctcgaaa caggagaaat agaaatgatc
     8221 actgagccaa aatatagcgt gggtcaaaat ctatatctaa ctttctattc agaaaataga
     8281 gaagatttaa caggagactc actagatatt atttcattga ttcaaaatcg ttcgattcgt
     8341 tttcagagaa tggatcccaa tcatttaaaa ctagagaacc aagatcgcta tatcgatcaa
     8401 ttggtattta cgtttagacg attattgaag agtgattgtc atggctaaaa atagttggga
     8461 gctaaaaata aatggacatg atgaacttct tgtgcggatg gaacgctatt caagcgagag
     8521 cgaacgactg attaacgaag cattgaaatc aaaaggttcg gctattgcag tggataggat
     8581 tacagaaaaa attcctgttt ctgaagcaga tttaagaaga gggcaccaac acgcaaaaaa
     8641 tagtcgtcca cttaagactc aatacattaa tttgggtttc atcattagac ctacaagaaa
     8701 atttgagtat ttaaaatatc ctgatttggg gataggtact tctaaaagaa atcagccgga
     8761 cgaatttatg agaagaggat taggtcttgc acttgatcca attacagaac ttctgattcg
     8821 tcaattcgat aaattaaata aataggggga acaacaatgg ctaaaacaaa aactgtagta
     8881 acaacgttcg ataacgtaag tatcaaacga attgctttta attttaagaa cgcaggaaat
     8941 gcaatcgcaa cgaattgtaa tggacaatta gatggcgaaa cagaaatgca aacggtggtt
     9001 aaaaaatgtg gagcgacaga agtaaaatca aaatctaaac caatcaatat gacggtaaca
     9061 attactgcac atgtaccgat ggaagtttat cgacgtttca atgggttgaa acaagatgga
     9121 cgtattaaac caggcattta ctcttacggt cctgattccg taggcgaaga tttctcactt
     9181 gctgcagaga tcgtggatga cttcgaagaa aatagaaagt tagttggtat gttagcatgc
     9241 acttcgaata caggattaac attctctatt gaaaatggtg cggatgaagt agctgcgtta
     9301 gaactagaaa caaaagttat gcaagatgaa tttggtaaat tttatcatga agcaattgtt
     9361 gcagaacttg aagaagactt aacagatcaa tggatgacga atctatctgc tgatgtgatt
     9421 aaaaaaactt cagttgtgac aactacggcc actcaatcac agtaaaaaaa acggaggtag
     9481 cgaaatgaac gaagattact caaaaattga actaaacgat ggaacaattt tgaatttaga
     9541 acctaaactg aatatcaaga aattattgat gatcaataga gattttaaca cagacgagtt
     9601 tgcaaaaatg actgtgggaa aaggatccat ggatatttct gttattcaag gtgcaaaggc
     9661 tgtgtatatt gcttaccgcc aagcgaacat gactgattat atttcattcg atgaatttat
     9721 cgataaatgg gattttgata tggctactgc cagctatatt tatcaattga tgatgttcaa
     9781 acaagcacgc gatgcttatc aaaaagaatt tgaaaaagca aataaggaaa aaaagcttca
     9841 aaagtaaaaa ttccaaagct cttagttgaa acgtgggtcg atgtctattc gatgttgacc
     9901 gacgtttttt ctatgccttc agatttggtt ttaagcgata tctgtttaga tgacattttg
     9961 caaatggctt acaacaagag tgcttatgaa ggatggaaaa actatgcaat aaaccaatcc
    10021 cagaaaaact aaagaaagga ggtaaaaaat ggctaaaaag agaacagaag cagaagtaac
    10081 tttcatagct aacgatgacg gattgaaatc tacgttaaaa gaaatcagtg ctgaattaac
    10141 taaaaataga gcagaattaa aactagaaca agctcaatta caacagactg gttctgaatc
    10201 agacaagtta ggaagtaaat tatcttcttt agaaaagcag tatgaattac aaagtcaaaa
    10261 agttgaagta actagccaac gtttagccaa tgcgaaaaaa tattatggag aaaattccac
    10321 cgaagttcag aaacttgaaa gagaactgat taatcaacaa acagcgcaac aacgtttgtc
    10381 aaacgaaatt gataaaacga gtaatgcact agctcaagca aaaggcgaaa tacagacgta
    10441 cgagtctaca atgcaacagt tggatagcga acaaaaaaat gttcaagcta gtgcttctct
    10501 gattgaatcc gaatacaaaa aatggcaagc aactgctggt caatcagctt ctgaagccga
    10561 gaaattagcg aaagcccaag aatatgtttc tcaacaatct gaaaatgcgg agaaaacgat
    10621 agatatccta agacgacagt tagaagctac acagtctgaa tttggcgcta catccacaga
    10681 agcaatgcag atggaagcga agcttaatga tgctgaacgt gaatttgaag agttaggaca
    10741 agctgctaaa aatgtagata caactaactt ggacgatatc ggaagcaaaa tagatatgaa
    10801 taatttaatg gaagcttctg acgttttaag cgacattggc gataagctta cagaattagg
    10861 gaaacaagca gtggactctg ctaacagtgt aggtagttcc cagagtaaga tacaagctaa
    10921 ttttggtttg tctaaacaag aggctgaaga attaacgaat gtagccagag acatttatta
    10981 taaaggtttt ggagaatcgt tagatcagtc cacagatgca ttgattttgg taaagcgtaa
    11041 tttaggagat ttaaataatc aagatttaca aaatatcacg gaacaagcta tggtcctaga
    11101 aaacaccatg ggcgctgata tggatgaaac gttacgtggt gtaaatggct taatggtcaa
    11161 tttcggcttg agtgctcaag atgcaatgga tttaatggtt tcgggtactc aaaacggttt
    11221 agataaaacg cacgaattag gcgacaatat ggcagaatat agccaattat ggagtcaaat
    11281 gggatattca gctgatgaaa cgttcggaat gcttcaaaat ggtttagatg cgggtgctta
    11341 taaccttgat aaagtcaatg acttagttaa ggaaatggga atatcgttaa cagatggtcg
    11401 atttgagcaa aacatggata tgtttagtga aagtactaga aaagcttttg aagagtggaa
    11461 aaatggcgga ggaacacaaa aagacgttat taattccatg attcaagatt ttagcaatat
    11521 ggatggtcaa tacgaccaat taaataaagc ttcgacaatt tggtctgcgc ttggcgaaga
    11581 taatgcgatg aaagttgtcc aatctttaac tgatgttaac catacatttg atgatgttag
    11641 tggatctgca caaaaaatga atgaagattc tactactccg ttgcaagagt tgaacgggaa
    11701 aatagctgaa ttaaaggatt cattagctcc tataggcaac acaatcatag atgcactcga
    11761 accagtaatt gattttctag gaaagatggc tgatgcgttt aataatcttc cacaaccagt
    11821 acaggatttc attgttgcta taggtggtct tacagcagca tttggcatac tgttaccggc
    11881 aatactagcc gtgtcatatt tatttggtcc gatgatgctt ataattggag gaattatagc
    11941 tgttatagca ggcgttattg tggcaattaa gaactggggt gcaattactg actggtttag
    12001 tggtttatgg aaaaaattta ctgattggtt gggtgatact tgggaaagta taaaagacgg
    12061 agcctcatca gtttgggatg gggttaaaga aacctggtct ggatttgtag attgggttca
    12121 agatatttgg caaggagttt ctgattggtt tggagagcta tggagcggat tagttgaagg
    12181 agcttccaac atctggcaag gagtccaaga aacttggcaa acattcgttg attgggtttc
    12241 aaatatttgg aacggagtca aagaagtatg gtcgattatt tgggcagaca ttgtaggaat
    12301 tgttcaaata ccatggacat taataacgtc attgattcaa gctggtatta atattatcgt
    12361 gggtattttt gatgtagctg gacagttatt gggcgcagct tggcaagctg tttggacacc
    12421 tatttctgat ttccttaaaa atacttggga tactatgaca caatggataa gtatcgcttg
    12481 gaatgggatt gtaactacat tccatactat atttgatcca gtagtggcat ggtggaatgg
    12541 tatatggaca gctattagta ctacggcttc aaatatttgg aattcaatta gtgcaacagc
    12601 ttctagtatt tggaacagta tcaagaatac aatcactagc ttggtacaag cagctgctac
    12661 agtaattcaa aatatttggt caactgtatc tagttggtta ggtggaattt ggaattcaat
    12721 cagctctaca gcatcaaata tctggaatag tgtgactagt agtataagca atgctataaa
    12781 cgcagctaaa agtgccattc aaagtgtttg gaatagtata tcttcgtgga ttagcggaat
    12841 ttggaacggt atcaaaaata ctgctttgaa tctttggaat ggaattacaa gcactattag
    12901 ctctaaagta aacgatggaa aaaatgcaat ttcaagcggt tggtccaatc taacaggtat
    12961 tgtttccgac atattcaata atgttaaaag tacaattgct aacatatggg aaggcatcaa
    13021 aaagactgtt agcgctccaa ttgattggat tagagacaaa atcagtggca tttttgataa
    13081 tttgaatatt tctataccac atattccgtt accggaattt atcatggaag gcagctttaa
    13141 cccgctaaaa ggtcaaatcc cccatttgcg tgtaaaatgg catgctaaag gaggtatctt
    13201 tactaaacca actttactag gtggaatgaa cggtgtcggt gaagcaggac ctgaagcagt
    13261 tttacctttg aaaagatctg ttttgcaaga aattggtgat cgtatcttga gtagcacatc
    13321 agtttcatct agggcacaaa cgattcaacc tgtgaataac tacgaattca atttcacaat
    13381 tgatggtaac gcagatgagg ttactatgaa gcaaacaact caacaaatca ttgatagcat
    13441 tacaaaaatt caaaatgata atgcttcggc atggcgttaa acaggagagt atttctcctg
    13501 tttttttagt attaaaaagg atgtgaaaaa atgactgatt gtatacattc tataatcgat
    13561 ggatttcctg attatttgca taaattggct ttagctgaaa gaccaaccat accttctcca
    13621 aaaagacaga gagttgaaac ttctgtttta ggtaggttag gtggcttagt acaagattac
    13681 tcgtttgaag atatgtcgtt tacattgcac tataactatt tagaggatgt ggaagaccat
    13741 caagcgttca agcaatcgtt ttatatcatg cgtcattggt taaactatgc aaagaaatta
    13801 gaattctctg acgatcccaa cgtctattac gttatccaga ctatcgatat tggggatgca
    13861 gaaaacgata ttgttgaatg gggagagttc gatgtaaata ttactgcgaa accattcgca
    13921 agagttcaag aagatgtacc tataaccgta gataaaccac agtcatttaa cttgctgaat
    13981 aatagtttag aagaaagttt tccaaagatt atcatcactc cttcagctac ttcatgccag
    14041 ttcatcttaa atgattatgt gtttagtttc gaaggcttag tagcaggaac tgatgtagtc
    14101 attgatagtg atttgatgct ttgctacgaa gagcaatcgg acggagatat tttagatcgg
    14161 tccaacaaaa tgaagaccat gcaatatccg acattgcaag tggatatcaa tcattttaat
    14221 tgcactggtt tgagcaaaat acaaatttat cgtaacgggt taaggtaggt gaaatagatg
    14281 atcgataatt taataactat ttacgataaa aatgacgcga ataatttagc tgaacattta
    14341 tatgatacgc aaggtttagg cgctttgtca gactggttaa cagctactgt tagcaataaa
    14401 ctaaacggag ccgagatatt tcagggtact tattcaataa acggaactaa tgcagatttg
    14461 attgtagacg gacgtattat tcagtgttat gtagatgaaa atcgagcaaa acagcgtcta
    14521 cggatttatt atgcaaaaac ttctgtaata ggcaatacga tagaagtaaa agctgaacct
    14581 attttcaatg atataagaaa atcggtgttg aataaatatg acagcggaac agaaaagatc
    14641 actgctactc aggcatggca aaacgcaaaa gttttagcga aaccagctat cccttcgcag
    14701 ttttctttct cgtcattagt agatacgctt gctaatgtga agatagaaaa ggcgaatttt
    14761 ttagaattct ttggtggaaa agagggatct attctagatc gatttcatgg tgagtttcta
    14821 aaagataata acacattacg tcatgaaaaa agtctaggta cggatcataa aatcaaagcg
    14881 atttatacta aaaacttaac tggtcttgac ttagagattg atgctcaaag tgttttagtt
    14941 ggagtttatc cattcattag cagctcttca gaaggagaag acgagatcac tctaccagaa
    15001 gaagttattt tcacggatta cgtggatgat tatcctgctg gatatgtttc ttttgttgat
    15061 tttaaagaca aagcgactga tgtagccaca ttaagggaag ctgctaaaga ctggttgaaa
    15121 acaaacatag ataaacaaaa accacaagtg agtggttcga ttgaattagt accattgagg
    15181 catcaaagag gctatgaaaa atttgttgat ctagaaaaag tttcgatggg tgacggagta
    15241 gatgtgtatc atccacagtt aaaagtgaat atgtcagcga gaattgtgga atatacgttt
    15301 aatgttctaa ccaattcata cgataaatta gttgtaggaa acgtcaaaac aaacttctta
    15361 gaaaacacag agaataatgt cagcaatttg attaatgatg ccattgatca attgaaaaac
    15421 ggtggcgaaa tcagtgattt actcaatgat attgtagatc atcaaactga tatgattact
    15481 ggtcaaaatg gtggttatgt tttattagat cctaaagaag cgcctagtcg tattttgatt
    15541 atggacacac cagataagaa taccgcaagg aatgttttac aaatcaacaa tgctggtatt
    15601 ggtttttcta aaactggcat taatggaaca tatgaaacgg catggacgtt agatggcgga
    15661 ttcaatgcct cgtttattac ggctggtgaa atagtaggaa ttactattag aggtactaca
    15721 ttaattagtg atggtgctga ttatagaaca agtattgcta atggcaaaat gacttggtac
    15781 tcaaaaaaag ttaacaaaga tattatggag ctagaagcac gtgattatgt aagtgctgat
    15841 gccggtattg tatcatacac catgaaaact ggtggtggtt tcatgattag aaatccacag
    15901 ggtaacttgg tttttagtac gtgggataat ggtaataaca gaccgtttct atcttttggt
    15961 gcgcccaatt tcaggtatag caatgctagt tatgtaactt ctggcgacgg tagttcttta
    16021 agcattaatg gtagtgcggg taactcatgg gaatttaagg tagctggtag gactatgaaa
    16081 tttactagtg atggtatgct aacgttacca ggttgttttt ttggttcatg ggaagatggg
    16141 aaacttgcta ggtttgaaca atcaacggta caagtatata aagattttac tgttagaggt
    16201 actaaaaact caactgtacc aacagaacat tatggacaac gactattgaa cgcttatgaa
    16261 actccagaat attatttcgc tgattatggg gaagccgtta caggtgacaa tggtaaagtt
    16321 cgtgttgata ttgaccccat gtttgctgaa acagtaaatc taagtcggta tatgacacat
    16381 gtgacaccta cagaactagt tttgtgtgct gttactcatg aagatattga ccatttcatc
    16441 attgaaacta gtaagccaaa cgtattagtt agatggaatt tagtggcaca ccgtctaggg
    16501 tatgaagata ttagattaaa agaggataca gcatatgata gcacagtgct tgaccaaaaa
    16561 cgtttttaaa acgaagacaa ggaggtatat aaatggctag cagtttatat aatttggctt
    16621 tagatttcag caaagaatta aactacacca aagctattat ggctcgtcaa ggtgataaag
    16681 ggattacggt gacggttaaa ccgtttctaa atggcttgca gatggatacg agtggcggaa
    16741 catttacttt aaaaggaaca acaccatcta accgttacgt agataatgtt gcaactagtg
    16801 taactagtga agaagtcacg ttttctcttg atggcacatt tatgagtgaa gcaggatatt
    16861 ataaacactg ctacgtagaa tatagaaaag acaatcaaat tttaacaacg caagatatca
    16921 tttttttctc actaggagtg tctgacattt cgcaaggcca agccgatgaa tatgtttcgc
    16981 aattagaaga gttgattcga aagtacaacg aaacttttga tgcttttatg gctgaaatca
    17041 aaggtagagt ggatagctta aatcaacaga ttactgattt aactggtcaa gctaaaacgc
    17101 tacaagacaa gttagatgct ctgaaagaag aaatttctaa gttaggtaac ttacaagtga
    17161 tgtacagtaa cagcatcgac ttcggggact atgattattc ggagaacccg aatttaatgc
    17221 catatattac agaaccttgg gttggacctt tgttaggtaa tggacataca gttaaagata
    17281 gtgttaaaag ggttataact catactaaaa ctagaacagc taattccggc gacatattat
    17341 cgcttggctt aggtattccg tgtacagcgg aagctaataa taggtatctt ataaccactt
    17401 tacgaccatc tacgacatac acattaagcg ttactatgag tgtcggttca gattggactg
    17461 gagagaccaa tactataggg gttaggctta gatatttaaa tgaacaaggt ggtatagaac
    17521 tgcccattaa tgcattaata ccagcaaatg tagaacgcga taaaatggtc actcatacat
    17581 ttactggtat aacaaaagat aatgtaacta gcataacaaa ctgttatgtt gaaatattct
    17641 cacttaatag tgaatataaa ggaacggtta gtgtcagcta tgacgttaag ttaaaagcac
    17701 actaccctaa cttactagat ggcccttact ggttaggtaa agttccttta ggcgagaata
    17761 ttgctgaccc tacagttgtc tttccacaca aaactagtga atatatggtt tatggtagac
    17821 gcaatacaga aaactatatc gctgaccaaa catataccat atcaatgaaa gcaactaaac
    17881 taacagttca atcgtttgcc gtgtatattg ctgctggccg cgttaaagta ggagacatga
    17941 agcctactga ggggttagct aatacatggg agttaacttt tactgtaact aagcaacata
    18001 tagatagtgg ggtaactaat tatctggaaa tataccaata cccgtcagct acaaaaggag
    18061 ctgttcaaat tgagtggctc aaactagaaa aaggcaatac tcgaaccccg aatattagtg
    18121 agtataaata tcgtggtact ggtatgcgtg attcaaacaa tccaaaagat tatgtttggg
    18181 atctagcacc agaatatgtc gaagataatt tggccacaga tattaaaatt tctgaaatta
    18241 ctggtaaagc aaacaattat accgatggga aagtatcgga gattaattcg cagttgactg
    18301 cttcaattaa tgaagtagac accacagcta aggatgctca aacaaaagcg aatgctaatg
    18361 cgactgctat agatgaatta gacaataaga tcgatgaacg cattaatgat acagctacta
    18421 ccacattaac agttacaaac gggaataccg gatcagcaaa gctttatcgt gaaggaaaaa
    18481 cagtttctat atattttgtg gctttaaacg gaaaaagcag tggtggaaat gattcaacga
    18541 tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg gttggctcga
    18601 tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga gccattaaat
    18661 ggcgaagaaa ctcaagttat ggatcggatt atacctttgc aattacttac acgatttaga
    18721 aaagcgtgaa tcgatatgaa ggcagcatat agaccaattg aaccttacgg attcgagcaa
    18781 atcattgtga atgatgaaga acatttaccg gaagaatgca cagaagtcga accaccgatt
    18841 ccaaattgga aaccgaaatt caattactgg gagggaaata aatgaaaaac atttggaaat
    18901 atggacgtac tggcggagag tacgcaggaa aagtattgga cgacatgctt gtatccgttc
    18961 cttacacaga tcagccaccg cttgaaggaa ttcgtgctga tggcgaaccg ttaacaatcg
    19021 ctgatcaaat gtttgatcct aaattgaatc aatggattat tttagcgaac gcactagatc
    19081 acaacgattt aaacaatctc aaagcaatgt atgagtcgtt agaaaatgag aacggcgatt
    19141 taaaacagat caatgccaaa ctcatgctaa gcgatgtagc aattaaacag gaaaatactg
    19201 cattgaaaga aaaagcggat agtttagcac aaatcaattc aaaaatgatg cttgcttcgt
    19261 tacaaaatag caaagacatt tcagaaatta aagagcaact aaatccagct tcaaagggag
    19321 gtgagtagta tgtttagttt tagcgatgtg aaaatgatgt atgattgggg ctgtttcact
    19381 aacgaacaag taatggtttt cgttccgttg tgcattactg aagaaaaagc agataaaatc
    19441 attagcaaag aagagagcgc atcttaattg atgtgctttt tattttgatt caaggagttg
    19501 tcacatgatt aatttagggg aatggggagc gatagcagga tcaataaccg ctatcgtttc
    19561 tttgatttta ttagtaataa aaccaattac tgcatctttc tcgaagatta ctgagactct
    19621 ttcaaaagta agtcacaatt tagatttgct gactaaagat ttagaatcga gcaaatcaga
    19681 tcgattgatg attcatgaag aactaaagaa acacgatgaa agattagata cacatgcaga
    19741 aaaattggta gaacacacgc aacaaattaa aactttattt agggagagaa gaaaatgaat
    19801 aataaaacgt tcgaagtact aaaatggttc gcactggtaa ttattcccgc actagctact
    19861 ttcgtggggt tagttggtaa agcgctcaat tggcagtaca cagatatctg tgttgtcatc
    19921 attactggtt ttggcgcgtt tttagggagt gtgttgggtg tatcaaatcg aacctacaaa
    19981 atgttctcgg ctgaaagcga agaaggagga aacaaatgaa aaagaaaatt actattactg
    20041 cgatgagcct gttaacggct ctttttttat tacccattaa tacgtttgct tatactatta
    20101 atgacgagta taatttagcg ccgaatcaag gagactccag attagcaatt cctaacaaaa
    20161 ttattttgca cgaaactgga atagatgcac cagcaagaaa cgtagccgcc aacatgaaaa
    20221 ataattataa cggaagcaat tcttatacta cagatgttat tggtgacggt gggattgttt
    20281 accgtgtggg tgagcaagga tatgtttcgt ggggagctgg taacgctaat ccttatgcgc
    20341 ctgtacagat tgaattacag cgcacatatg ataaagcatt gtttgaaaaa aactatcgag
    20401 cttatattga atatacaaga gatagtgcaa aaaaatatgg aattccattg actcttgatc
    20461 aaggaacttc tttatttaca aaaggaatca tttctcattt gtgggtgaca aattatgttt
    20521 gggggaacca cacagatcca tatggttact tatcgcaaat gggagttagc aaagaaaagc
    20581 ttgcttatga tttagctcat ggatttaccg atgaaaatcc aacaacttct gaaaacaagc
    20641 ctgtcattga tccaacacga gctggtgcag ctaatcctac gctgacagat ggaacaaatc
    20701 acgcccacat tgatcagttt ggggaaatcg aaaacgcaaa cttgcatgtg gctggatggc
    20761 acattgctaa ctataaatac gagtatattt tcattatgga ctacaatact gggaaagaat
    20821 tagctcgagt aagagctgat ggaatttata gaccagatgt aaaccaagct tataatactt
    20881 ctggaaatgt tggttatcat gtatctttca atatgcgtaa ttttcctagt aagaaagtct
    20941 atgtaatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
    21001 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcg
//
LOCUS       DAZKCY010000041        20754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000041 DAZKCY010000000
VERSION     DAZKCY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.041"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(590..1615)
                     /locus_tag="ACG8XC_001374"
     CDS             complement(590..1615)
                     /locus_tag="ACG8XC_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9191081.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1890..3575
                     /locus_tag="ACG8XC_001375"
     CDS             1890..3575
                     /locus_tag="ACG8XC_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFP9191082.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3589..4872
                     /locus_tag="ACG8XC_001376"
     CDS             3589..4872
                     /locus_tag="ACG8XC_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9191083.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4942..5811
                     /locus_tag="ACG8XC_001377"
     CDS             4942..5811
                     /locus_tag="ACG8XC_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9191084.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5808..6644
                     /locus_tag="ACG8XC_001378"
     CDS             5808..6644
                     /locus_tag="ACG8XC_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9191085.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6670..7737
                     /locus_tag="ACG8XC_001379"
     CDS             6670..7737
                     /locus_tag="ACG8XC_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP9191086.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7734..8561
                     /locus_tag="ACG8XC_001380"
     CDS             7734..8561
                     /locus_tag="ACG8XC_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9191087.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8606..9631
                     /locus_tag="ACG8XC_001381"
     CDS             8606..9631
                     /locus_tag="ACG8XC_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9191088.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9628..11811
                     /locus_tag="ACG8XC_001382"
     CDS             9628..11811
                     /locus_tag="ACG8XC_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFP9191089.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11811..12941
                     /locus_tag="ACG8XC_001383"
     CDS             11811..12941
                     /locus_tag="ACG8XC_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9191090.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12938..13615
                     /gene="pgmB"
                     /locus_tag="ACG8XC_001384"
     CDS             12938..13615
                     /gene="pgmB"
                     /locus_tag="ACG8XC_001384"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9191091.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13699..14226
                     /gene="msrA"
                     /locus_tag="ACG8XC_001385"
     CDS             13699..14226
                     /gene="msrA"
                     /locus_tag="ACG8XC_001385"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9191092.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14427..15113)
                     /locus_tag="ACG8XC_001386"
     CDS             complement(14427..15113)
                     /locus_tag="ACG8XC_001386"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9191093.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15193..16731
                     /locus_tag="ACG8XC_001387"
     CDS             15193..16731
                     /locus_tag="ACG8XC_001387"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP9191094.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16851..17318
                     /locus_tag="ACG8XC_001388"
     CDS             16851..17318
                     /locus_tag="ACG8XC_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFP9191095.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17852..18280
                     /gene="rplM"
                     /locus_tag="ACG8XC_001389"
     CDS             17852..18280
                     /gene="rplM"
                     /locus_tag="ACG8XC_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP9191096.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18296..18688
                     /gene="rpsI"
                     /locus_tag="ACG8XC_001390"
     CDS             18296..18688
                     /gene="rpsI"
                     /locus_tag="ACG8XC_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9191097.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18777..20003)
                     /locus_tag="ACG8XC_001391"
     CDS             complement(18777..20003)
                     /locus_tag="ACG8XC_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9191098.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
BASE COUNT         6685 a         3732 c         4338 g         5999 t
ORIGIN      
        1 ttaaaaaaga ggttgtgaca aattattgtc acaacctctt ttttggtttg tctaaaataa
       61 tttgtctaac tagaagaaaa gtgggaaagg atcttaacaa catcggttcg tatccaatta
      121 aggtatcgga acgatcatct tccaagagaa gtcaatcaac aaaagtaaag atagatcatt
      181 ttttgaatgc aaaataaatt gatgggaaga tagagcggtt atttttaata ttttgctagc
      241 tattattgaa taattcctcg aataaaacaa aaccagtaga catgtctgtt attctattta
      301 tgttttagag ataagtatcc ccaaataaca tggattgaaa taagggtaaa tgtgtttaaa
      361 aaaacgaact ataaaaactt atacacaacg tggttaaaaa tagttttttc ttccctgtgg
      421 aaaaaatagt ttaacacaaa cattatagtt atccacagct atctaaaaca gatggaaaat
      481 aagaaaaatt cgctaaaata ccgggagggg gtaacgaggg atgagtgatt gaaaaaagaa
      541 cagagtaact gacttatcct cagtgtttct gttcttttat aattctgttc tacatttctt
      601 gttgttggat cgaatctgtg agagtaatct cggttgaaag aatgattctt ttttcttcag
      661 ggtattggtg gtggattagt tggaataaaa gttctgtggc tttgattcct aactgatttg
      721 tcggaatgtc tatccgagtc aactgtggcg aaaataggga ggacaataag ggatcactga
      781 atcctgcgat cgaaagatca tcaggtatct gctttccaga ttgttctgcc actttgtaaa
      841 tactcaatga ttttaatgca tcggtagaaa aaatcgcagt tggtctttct cgtagcttca
      901 ttaaatcatt cattgctgcc atgctatctt ctaagcgaaa gccgccgtct ataatccatt
      961 cattacggat atctaggtgg tgatcaaata agcattgacg gtaaccattg agacgatcat
     1021 tggatacata aacttctgtc ggagcatgga gacagccgat atgacggtgt ccattcttga
     1081 tcagtgtttc caccaaatcg taactatcct ggaaattatc cgtgtccact gagtagatgt
     1141 tcgtgtagtc gtttccgact tttccgatta ccacaatagg aatattgtat tgatccaatt
     1201 caaaaagaag tgtttcatcc atcgtagagc ttaaaataat aattcctcgg atcatttttt
     1261 cttggatctt tttcgcgcat ttttctagct cttcttctgg tgtttttgcc gtttgtagaa
     1321 tcaagtcgaa gttattttgt tcagaaacgg cagaaatgga ttgaataaca tctgaaaaaa
     1381 aaggattgcc ataggtcgta tgagaagtac gatgagataa tgctagaatc gcgtcaaaac
     1441 catttcctga cagtcctctt gcaattttgc tcggttcata attcaattct ttgattgctt
     1501 gaagtacact tgcttttgct ttatcagaga tatttggctg gttatttaat actctggaga
     1561 cagttgattt tgagacatta gcttttttcg caatgtcata aatagtaggt ttcaatttta
     1621 tatgcacctc ctttttggga gcgttttaga ataattccat agtacaatag acattctttt
     1681 ttgtctatga ttttgaaaat ttaggaatag cttgcatgat catgagagac catatttttt
     1741 taagaagcta ttaattattg atacataagc tttcttattt gtatttttgt gtatatcaaa
     1801 attatttaaa aaaatgctat tgacagtaag cgttttccat aatatgatca aatcgttggg
     1861 aacgctccca aaaaagaaag gggaatccga tgtatacaaa acttttacaa gcgatttatc
     1921 gcgactcaga tttgtctgag aaaaccgcct ctatgattga acaaaaagtc agtgtttatc
     1981 agcagaagaa aggccaggca ggcaaactgc attgggacga agaagatatt ttcttgatta
     2041 cttatgggga tcaatttttt gaagagcagc aaccaacttt gcgtacattt aagaaatttt
     2101 atcaaaagta tttggcagat acatttagag ttgtacattt cttgccattt ttcccatata
     2161 catctgatga tggtttctcc gttgtaaatt atcaggcaat caacgaccgt ttgggcacat
     2221 gggaagatat agaagaaatg agaagcgaaa cacggttaat gttcgacttt gtctgcaatc
     2281 atatgtctgc cgaaagtact tggtttcagg aatatttaaa cttgaatgat acgtacaaag
     2341 atttttttat tgatttgcca ctggatacag atacaagcag cgtgactaga ccaaggacca
     2401 gcccgttact tacaacgttc attgacaaaa acgggaaaga aaaacacgta tggacgactt
     2461 ttagcgaaga tcaagtagac ttgaatttta aaaatccaga agttttattg aaaatgatcg
     2521 atattctttt gttttactta gaaaaaggcg cttcatttat tcgcttggac gctgtgggat
     2581 ttttatggaa agaactagga acttcttgta tacacctgcc agaaacgcat aaaatcatcc
     2641 aattattccg tgcaatcgct gatgaagcag ctccaggaac ggtattgatc actgagacaa
     2701 atgtgccaca caaagataat atcagttatt ttgggaatgg cgagaatgaa gcacagatgg
     2761 tctatcagtt ttcgctacca cctttgaccc ttcaagcgat tcgttcgggt aatacaagta
     2821 agttgaaaga atggttaaaa accatcgatt tcgcatcgaa aaaaacaact ttttttaatt
     2881 ttttagcctc ccatgacggt atcggattga atccggtacg aggtattctg ccggaaaaaa
     2941 cgatccttga attagtggaa cagctgaaga aagagggtgc ccgagtaaac tacaagaaaa
     3001 acccagatgg aacgaccagt ccatatgaga taaacgctac gtatctagat gcgttaagtt
     3061 ctccaacaga tacgacggaa tggagaagca aacgcttttt agctgctcat tcatttttgg
     3121 taacgttacc aggcgtgccg gcaatttata ttcaaagtgt gctgggaagt cgaaatgatt
     3181 acaaaggcgt agaaaaaaca ggagagaatc gaaaaatcaa tcggcaaaaa tttttgttag
     3241 cagatattga gaaagagttg cagacagatc cattaagaac agcagtatat ggtgaattga
     3301 cccagatgct atctattcga aaaaaagaaa aagccttcca tccgaatagt ttgatttact
     3361 tactggactc agcagattct tttatcagtt ttattcgcga agcgaaggaa gagaaagtct
     3421 tagttatcca taatttttcc aatcaatcag caggttatct tttaccaaaa ggagattacc
     3481 tagacctgtt ttctggacaa attatccatt gcagtcaaga acgaaagttg atattccagc
     3541 cgtatgaata tatgtggctg aaaataataa aataagaggt ggaaaaaaat gaaaaaaatt
     3601 attgttacta cctgtctgct agctactgga tttcttctta ctgcttgtgg tgaaagtcaa
     3661 agctctaacg aatcacaaca gacaacgatt caattcatgc actcttctgt cgaacaggaa
     3721 cgattaagtg tgatcaatga actggttgct gattttgaaa aagaaaatcc agacatcaaa
     3781 atcaagcaag tgccggtaga agaatcagcg ttcaatacaa aaatcgttac gttagctcgt
     3841 tccggtaaat taccagaagt aatggaagtc agtcaggatt ttgctaaagt gatggataaa
     3901 gatgaactga ttgatcagac ggcagtccaa aacgttatcg aggaagctgg agaagataat
     3961 tattatgacg gagccaaaaa ccttgttcgt tcagaagatg gaaaaagcta tattgctgca
     4021 ccaattagcg gctgggttca aggtatttgg tatgacaaag aaaaattatc agaagctggt
     4081 ttttcagaac cagaaaattg ggatgatatt ttaaaaattg ctcaacattt cacagataaa
     4141 gagaataaac aatatggaat tgctatgcct actgctgaag gaacaatgag tgagcaagcg
     4201 ttctctcaat ttgctttgtc aaatggagca aatgttttgg atgatgaagg cgaggtaacg
     4261 atcgatacgg aaaagatgag agaagcattg tcgttttatc aaaacttatc ccaatacacg
     4321 atgccaggct ctaatgatgt gacagaaatc aaagatgctt tcatgaatgg aacagcacca
     4381 atggcaatct attcaactta cattttgcct tctgtttatg aagcggggaa ctcagaaaat
     4441 atcggttttg ctatcccaac acaaaaagat caagctgtct atggcacagt atctggattg
     4501 acgatttctt ctggtctaga cgagaaacaa aaagaggctg ctgaagcatt cacagctttc
     4561 ttatctcaac cgaaaaacat ggaaaagtgg gtcttgatgt ctcctggtgg tgctcaacca
     4621 gtgaataaac aagtagtcga actggatggt tacaaagaaa atgaagtcat caagtctttt
     4681 ggcgagctgc catctgagat tgctagtgct tttgatgaag tacaagtctt tggactagtc
     4741 ggggaaaaga atttcacgaa aatgggagat atcacaagct caggtgtgat cggaaaagca
     4801 gtgaaccaag taactgtcgg aaacgaagat gtcgatcaag ctttagctga cgcacaaaaa
     4861 aatacgaaat aacaagaagt tctgccaatg gtgttcgaaa aaagtagata gctattggca
     4921 gattatcagg gggaggtttc aatgaatcgt ccaaggaaac attctgactt aaaattagcc
     4981 atgggacttt tagcaccaag catgctttta ttagctgttt tagtgctgat tccaatgctt
     5041 tccaatattc agatcagttt tttcgatatg ccgctgaatc ctaactcaga cgccgttttt
     5101 atcggattaa gcaattatct tgaaatttta agtgatagtg aattttatcg ttctttaggt
     5161 ttaacagttg cttacacatt cttagttgta gcaggcagta cagctttagg attagccgta
     5221 gctattttgc ttaaccgcaa gttcagattt aggaaaacgg tgcgttcact gattttatta
     5281 agttatgtta cgccatctat ttctttgatt tttgcttgga aatatatgtt caataatagt
     5341 tatggaatcg tcaatgatct gttagtagat aggttgcagc ttttcaacga agcaccgctg
     5401 tggttcgacc agccagttgc tagcttcttt atggtcgttt tgtttgctat ctggagatac
     5461 tatccgtatg cgtttatttc atttttggct attttgcaaa cagtcgacaa aacgctttat
     5521 gaagcagctg aaatggacgg tgcaaatgct tggcagaagt tcaagatcgt gacactgccg
     5581 gcaatcaaac cagtactagc aactgtgatt gttttacgat ctatctggat gttctatatg
     5641 tttacagatg tctatctact gacgaataaa gtcaatatct taggcgttta tttgtatcag
     5701 acagcctttg cgttcaatga tcttggaaaa gcagcagcta tatcgattct attgttcatc
     5761 attattgcag cggtcgtctt gctctcacga aagaaggtgg acttgaaatg aaaaataaaa
     5821 agagcaggaa aatcttattt tacgcgacga ttttgttgct attagctgtc aatttgtttc
     5881 catttttgat catgctgatg acatcattca aggattcaca agaagcaatt gcaacgacac
     5941 aaactatttt tccgaaagaa tggacttttg aacattatca ggatattttc aatgctgaga
     6001 tttttccatt catcatgtat ttcaaaaata gtttagtgat ctccttgata gcctccgtaa
     6061 ctgcagtagt gattgggatc agcggggcat acgctttgtc aaaactacgc ttcaaagctc
     6121 gctcgaaaat caatgctagc ttttacaccg tttatatgtt ttccggtatt ttgatcgttg
     6181 ttccattatt taaaatcatt tccggtctag gtttgtatga taccaataca gcactgatta
     6241 tcacgatggt cgttcaaaca cttcctactg cgatattcat gctaaaaagt tattttgaca
     6301 cgattccgac tgatattgaa gaagcagcaa tgatcgatgg tcttagccgg atccagatca
     6361 ttattcggat cattatacca ttggctattt ctggaatcat ttctgtcttc gtttattgct
     6421 ttatggtttc gtggaatgat tatttatttg cttcgatttt cctgtcgacc tccgaaaaat
     6481 ttactttacc aatcggtttg aacacattat tcagtacacc agactacatt tggggacgaa
     6541 tgatggccgc ttcattggtg accgctttac cagtcatcat tatgtatgcg ttatctgaac
     6601 gatttattaa aggtgattta gtcgaaggcg gcgtaaaagg ataaaaagaa aagaaagagg
     6661 aaagaaaaga tgaaaaaatt agttgcatta gaaccaagaa tagccggatt caaagattac
     6721 gaagaccgtg agatcgaaaa gaacgaagta cgtatccgag taattttcgc tactccaaaa
     6781 ccaggaacag aaattgtcga ctttagagga ttaagtccat ttatcgatga agaattttca
     6841 gaagaatggc gcctattctt gccaagagaa gaaggaagcc aacgcggtgt ggtatttggc
     6901 gaatttcaat taggaaatat gattgtcggt acgatcgaag aaacgggcag tttggtgaca
     6961 gagtatcaaa aaggagagtt agtctgcact tatggaccca ttcgtgaaac gctgattaca
     7021 aacggtgttg ataattataa actacgtaaa cttgatcggt tggatcaatg gaaaaatgca
     7081 gtatgttacg atcctgctca attcgcttta ggtggtatcc gggaagcaca tgttcgagca
     7141 ggtgatgctg tagcaatctt cggtttaggc gctatcgggc aaattgcgat tcaattagct
     7201 aaaaaagcag gtgctactac catcgttggg atcgatccca tagcgcagcg tagagaatta
     7261 gcagaaaaat acggtgccga ttacacgcta gatcctatga aagcagatgt cggttttcgt
     7321 ctaaaggaat tgacagataa acgtggagta gattcagtta ttgaaacgag tggcagtgct
     7381 gccgcattac aagcttcttt aagagggatc ggctatggcg ggatcatttc ttatgttgcc
     7441 tttgctaaac cttttccagc tgggtttaat cttggtcgcg aagcacattt caataatgca
     7501 aaaatcgtct ttgctcgagc atctagtgaa ccaaacccag attacccaag atggaaccgt
     7561 aaacgaatcg aggacacatg ttgggaatta ttgatgaacg gttacttgga ttgtgaagaa
     7621 atcattgatc cagttgtgcc atttgcagaa agcgcagaag cttacatgcg ttatgtagac
     7681 cagcacccag aagaaagtat caaaatgggc gttcaagtcc gaggaggaga acaatgaaat
     7741 tcggtacgca agatcaagca tttttcccaa gagacatttt agaaaaattt agatttatca
     7801 aagactgtgg atttgaagaa tacgaaatcg acggtaagtt gttaatagaa aatatcgaca
     7861 cagtcaaaca ggcaatcaaa gaaacaggga tcccagtagt gacagcttgt aacggctacg
     7921 atgggtggat cggtgacttt atcgaagaac ggcgtcttaa tggtgtacgt caaattgctg
     7981 agattttgcg tgcactaagc gaggttggag gcaaaggaat cgttatccca gcagcttggg
     8041 gaatgttcac ttatcgatta ccgccgatgg tttcaccccg aagcaaagaa ggagactata
     8101 aagcagtcag cgaatcactt cgttatttag ataaagtagc cgaagaaaca ggtacggtta
     8161 tttatttgga gcctttgaac cgataccaag accatatgat caatttgtta gcggatgcgc
     8221 gtcattacat tgactctaat caattgaaac atgtgcagat catcgctgat ttttatcata
     8281 tgaatattga agaagatgat atttcaaaag cattgactac gcaaaaagag tatgtgggtc
     8341 atatccattt agcagataat catcgctacc aaccaggatc tggcgcaatt gattttactc
     8401 gccattttca aacgctactt caaaatgagt atgaaggtta tatggtatat gaatgtcgtt
     8461 tgcgaggaga aaatgaagtg aaaatttatc aggaatcatt gcaatacttg aaaaactgtc
     8521 tggaaacagc gcgtcattta aatgatttag agaaagaata gagaaagaat aacaataagt
     8581 aaataagcaa aggagggaga aataaatgaa gaaagtagca atcataggcg ctggacaagt
     8641 agcagaaaaa gtacatgctg cttattatca aaaaagatca gatgtcttag aattggccgc
     8701 tgtggttgat ccagtcagcc agcgagcaga agacttttgc cgacgtacag gattttctca
     8761 tgcgtataca tcagttacac aaatgctgaa tgagatttct cccgatatcg tgagtgtctg
     8821 tacacccaat cgctttcact tcgaacatgt attggctgcc ctcgaagcta atgcttcagt
     8881 gatgtgcgag aaaccaccag ctatgacagc tgatcaagca agaaagatgg cagagacagc
     8941 cgataaaaac gggcagattt tggcttatgg attccagcat cgtttttctg acgaagcaac
     9001 aatgatcaag aatcatcttg atgttctcgg ggatatttat tacgtagaag cgaatgcgct
     9061 tcgtcggtct ggtgttccag gctggggaac gtttatcgac aaagaacttc aaggaggcgg
     9121 gcctttgatc gattatggca ttcacatgct tgatacagcg atgtatatta ctggatttcc
     9181 taaagtaaaa agcgttcggg cgtacagctt ccaaaaaatt ggtacgaaaa aaaattcagg
     9241 gaaattcggg aaatgggatc caagcaaatt cacagtagaa gatgcaattt ttggcacact
     9301 ggaattagaa aacggcaaga tcatccaact gaatacttcg tttgctttga atatcagaga
     9361 agacaaactg aatgtggctt tttgtggcga tcaggcaggt gctaatctgt atccaggcga
     9421 actattcgaa gatgtagatg gaaagctggt ttccttacaa aagaccacgc cagttaatga
     9481 cccacatgaa ctaagtatgg ctcattttgt gaatcgggcg ctcggtgacc agactcagat
     9541 gattgcagat ggctggcaag gatatgtcat ccagaatctg gtggaaaatt tatatcgttc
     9601 ggcagaaaca ggagaagtta ttaccttatg aaagtagaag aaaaacagtt tgatttagag
     9661 tatatcaata agtatgcgag tcagatgacc attggaaatg gattttttgg tatcagagga
     9721 agccaggagg aagactatcg gaatcaagta aggggaatgt ttgcagctgg tgtatataac
     9781 cgacctttag gagcagaatc agccgagtta gtgaatctcc cagatgtcat cagatgggaa
     9841 ataaaaatcg acggtgaaca tttttctctt gagacgatga aagttcttgc atacgagcgg
     9901 gcactagatt tagaaatggg agagcttaaa cgctatgcgc tcattgaaac tgctcaagga
     9961 aaaaaagtgg aagtcattac ttgtcgtgtt gctgatcaaa ataatctcca tcaggcagcc
    10021 ctgaaagtgg tcatcaagcc gttgaccgaa gactgccaag tagatgtgaa aacgggtatc
    10081 gatggacagc aaaccaattt tggtacacag cagttggttg aaaaagaact gcgagtattt
    10141 ggtgacgatt tgatcagtgc atcctaccag acaacagaaa gtaaaattga tattgggatt
    10201 gcagctaaat tcaataaagc aggtgtattc caagcaaaaa atcgccggat caccgcttcc
    10261 ttttcagaaa agatcagaaa aaatacgtcc tttacgctgg agaagatcat cgtgctctct
    10321 acctctcttc agcaaacaga cccgtttaag aaaagtattg cccaagccca gaaaagcgtt
    10381 agttatacag aaattttaga gcagtcgatg tcttattgga cagatttttg gcatcagcat
    10441 cggataacga tccaaggcga ttcttctttc gatcagttag cgatggactt cgctgcttac
    10501 catttacaga ttatgactcc ccgcaatgat ccaagtgtta gtgtaggggc gaagggacta
    10561 actggcgaag gttataaagg gcatgttttc tgggatacgg aaatttttat cctgcctttc
    10621 tttttatatc agtttcctga agtagcaaag aatttgctgc tgtaccgtta tgatcgatta
    10681 gaaggcgcac ggcaaaaagc aagagattat cactatcaag gtgcactttt cccatgggaa
    10741 agcgcacaca ctggtcagga agagacaccc aaatttgctg cattgaatat caaaaccgga
    10801 acaagacaag aagttgcttc agctgccgca gaacatcata tcagtgcaga ccttgcttat
    10861 gccgttcaag agtatgtcca agcaacagag gatcaatcgt ttttagaaac aaagggaaaa
    10921 acgctcattc atgaaacagc agctttttgg ctgagcagag ctgtaaaagt cgatgatcat
    10981 ttagagatac acagagtcat tggtccagat gaatacacag aatatatcga caataatgca
    11041 tatacgaact atctggcgca ttataatgtg gccttagctg ttaaatatca agctggcgat
    11101 ccgctttttt tgaaagaatg tcaaaatttt ttagataagt tatatttgcc agtgcctaac
    11161 aaagaacagc tgattccgca agatgacacg tatctcagta agccagttat tgatttgacc
    11221 acttacaaag aacgtcctgg cagccagtct attttacttg attatgcccg tagcgaagta
    11281 aatgaactcc aagtcagcaa gcaagcagat cttgtcatgc tgttatactt attacctgag
    11341 tatttttctc aaccaatcgt ttctaagaat ttgcattact atgaaaacaa aacaatccac
    11401 gactcatcgt tgagtaaagc gattcacgcc atagaggctt gccgtatcca agaaatggaa
    11461 tgggcgtatc atttattcca agaagcgtgc ctgattgatt taggaccaga tccgcattca
    11521 tcagatgacg gaattcatgc tgcttcatta ggagcactat ggctagcagt tatatttggt
    11581 ttttcaggta ttacagtaac gaatggaact ctttctattt ctccaagact tccttcagca
    11641 tgggaaaaaa tttcttttcc ttttatttgg aaaaatcaca ggttgctatt tgttttgaca
    11701 aaaaaacaaa tagtaataaa aaaagagacg aaagaaacat tacctatttt tgtcaatgga
    11761 gaccaatacc agctggaaaa agaactgatc ttggaacgga ggggaaaata atgcgtattc
    11821 tgattgcgcc agattctttc aaagaaagtc tgacagcctt agaggcagca caagccattc
    11881 aatcaggttt tcaagaagtt tttgttcagg ctgaatatga tctgctacct ataggcgatg
    11941 gaggagaagg aacggtttca tcattagcag acagtttaag gttgaagcgg caaacagcag
    12001 tcgttactaa tgcatttggc aaaaacaaag aaatccagta cgcttcagac ggtcaaactg
    12061 cgatttttga aatggcagaa atcgtcggat tgaaagatgt cccactaaac caacaacagc
    12121 cacttaggtt atccacgcga ggagtaggag aactgatacg acaattgttg caacaaggtg
    12181 ttcgacacat catcatcggc gttggcggaa gtagtaccaa cgatggcggc atcggcatgg
    12241 cagaaggtct aggttatgct ttttacgatc aagataatca aaaagtagaa gcactgggag
    12301 aaaatctagg taaaattgct acagttgata aaacgaacgt gcctattgag ctgattaaag
    12361 ccaaaatcac tgtcattact gatgttacta atcttttatg cggaaaagaa ggcgcttctt
    12421 ttgttttcgg accacaaaaa ggcttgaaag aaaatgaatt agcagtggct gaccaagcca
    12481 tgcatgattt ttatatgaga tttgcccctc aagtaatcga ggcatcgggc agtagtgctg
    12541 gtggtggaat gggtgcagga ctgttagcat ttgcttcagg gaaaatagtc tctggcatcg
    12601 actacgtatt ggatttactg gagttcgata aacgggtaca gcaagcagac ctcgtaatcg
    12661 taggcgaagg acgtctagat ggacaaagtc taaaaggaaa agcgccagtt gggattgcta
    12721 gaagagtacc agagggcgtt cctgtgatag ctatttgcgg aagtgttggt aaaggaagcg
    12781 aaaaagcagc agatgttggc atttcggccg tttttccgat ccttcctgca gctgtgcctt
    12841 tacaagaagc tttacagcaa gccactgata atcttcatcg cacagctaaa aacatcgcat
    12901 ctttacttca agtcgttcat tttcaaggag gtaatcgatg aaaatcaaag cagcactatt
    12961 cgatttagat ggcgttcttg tagacacagc tcgttatcat tacgaagcgt ggctggtttt
    13021 agctaaccag ctgtccattc cattcactga gaaagaaaac gagcaactga aaggaatatc
    13081 cagaaccgag tcactagagc gattgctgtc atttggaaaa atggaacaga aattctcaga
    13141 aaaagagaaa tcagcctttg ctgagcaaaa aaacaatcta tatcttcaag cgattcaaaa
    13201 aatggatgaa acatccgttt tgcctggtgc aatagctgtt cttgagtact tgaaaaaaac
    13261 aaacatcaag atcggattgg gatcagcaag caaaaatgca cgcctcattt tagaaaaaac
    13321 gaatttgaca agttattttg atgtccttat tgacggcacg caagtatcaa aagcaaaacc
    13381 tgatccagaa gtttttctca aaggagcaca gcaattgaat gtaccgccaa atgcatgctt
    13441 ggtcattgaa gattcagaag ctggttgtca agcagctttg gctggtaaca tgcatgtatt
    13501 gggaataggt gaaaacataa atcttccttc tgctgaatat gtgattcctg atttaaccgt
    13561 ttttgatcaa gtacgttcgt tttggcattt atcagaagcc gtatcacgta tataatagtt
    13621 tccgtttttt ctgtcacaag caaaacgtta tcctcctttt ggaaatcaag gtatgatgag
    13681 aaaaaagggg gattttatat gacaaaaaaa gcagttttcg caggcggatg cttttggtgt
    13741 atggtcaaac catttgaaga gcagccaggg atcatttctg tgacatccgg ttatactggc
    13801 gggcatgtac caaatccaac ttatgaacaa gtaacaacag gcacaacagg gcatacagaa
    13861 gcagtagaga tcgaatatga tccagaagtt atttcttatg atcagttggt ggaaatctat
    13921 tggcagcaaa ccgatccgac tgacgcatta ggacagttcg ctgatcgcgg tgattcttat
    13981 cgtccagtga tctactataa tgatgaagaa gaacgaaata ttgcagagcg ttcaaaacaa
    14041 gcattgcaag acagcgggcg attcgataag ccaatcgtga caaaaatcga accaagaagc
    14101 gaattttatc ctgcagaaga ttaccatcag gattattaca agaaaaacaa gctccattac
    14161 aatctttatc gagaaggttc tggacgtgca ggtttccttc gtcgacattg gggcgtcaag
    14221 aaataagaag ccgaaaaagc tacttatgga acaccgtata tcagaggttg taaaaccagc
    14281 attttgagct aatactgagg gtcagctgat tgaacaccat ttagtggaga ggttgtgaac
    14341 ctgtcgccta gctccaagtc acaaggattt ggtaagagaa cactatatat cagaggttgt
    14401 gacaagtcgt ttgtcacaac cttttttcaa tacctaagat gaaggacaat cagcaacgca
    14461 ctgctcaaaa aaggaatgaa aggcagcttt tccaactttt ttttatgaca gataaagtaa
    14521 acagaaaaaa cggatattcc aaacagacta gcaagaaaca agatctgaag caactcttct
    14581 agagacaagc caccactcca agaaagcatc aacagagtat cgccagctcc gagataggat
    14641 tgaccatagc gaaggcacac accgcctacg ataaacgtta acagcacact ttgccattgg
    14701 aagacgccgg cctgcaacca aaaaatccat aaaattgccc aagaacagaa tagcgtgtga
    14761 gaatggacca tgaagtaaaa taaatccatc aacgaaaata gcaaagccga aagcatccat
    14821 atgaacgtcg aaaaatttct tgcggaagaa aaagtaaaga accaggcaaa aagaccacca
    14881 cataaggctt ctgccaacaa atagtagaag ggcaaccgac acctgcaata gcggcagcga
    14941 aatcgttgga cgatgatcga gagaatcgga atcaattcat accaagaaag cgaacgacag
    15001 catttgacac aatgagatct aggataaata atagagtgac ctaacggaat ccgctgggcg
    15061 gttaaacata aaaatgatcc gatacagcaa ccaatgataa aataaagtaa cacgccatct
    15121 cccccttttc aaaaccaaga tacgcaagct taaactaaat ttttgtacat acataaaatt
    15181 tgaggtgaaa aaatgaacgt ttcaatcgga ttagatatcg gaaccacaca aaccaaagca
    15241 gttgctttca cagatgaagc ccaagaagca gcatccgctt attttcgcta ccctttgatc
    15301 caagaaacac aagggatggc agaacaagat caggaattga tctttcaagg tgtctgcacc
    15361 gttataaaag aagtaattaa acagttgcct tcagacacaa tcattcaatt tgtctccttc
    15421 tcctctgcga tgcatggcct gatattgttg gatgaagaga aaacaccgtt atctcgaatg
    15481 atcacttggg cagataaccg agcagcggac gaagcagaaa aactgaaata taccgtgctt
    15541 ggcaaagaat tttatcggca gactgggacg ccgattcatc caatgacacc attgaacaaa
    15601 atcaagtggc tgcaaaacag ccaacctgaa ttattcaaaa aaactgctta ttttgtcggt
    15661 atcaaagact atatctttta tagattgttt ggcgaactga tcagcgacta cggaacagct
    15721 tcaggaactg gatattttga tatccatgaa ttccaatggg cagaagaagt gcttgactac
    15781 ttgaacattc gaatagagca attgccgcaa gtctttcctt ccacctatca agtaacaggt
    15841 ctaccatcaa aaacagcagc agaactaggt ttgccaaatg atatatcatt tgttttggga
    15901 ggggcagacg ggccgctttc taatctaggg ttaggtgctt ttggtgatac gatcgccgct
    15961 ttgaccgtag gaacgagcgg agcacttcgt tttattacta ctcagcctca gttccatcca
    16021 gaaatggaga cgttttgtta tgttctggat cagaaacatt gggtagtggg aggagcgact
    16081 agcaatggtg ctggcatctt tgattgggca gggcatacat ttttacagga agtcgttcaa
    16141 tctgctaaag aaaaaggaga aaatccatat gatgcactgc tttcggctat cgcttttgtg
    16201 ccagcgggtg ccaatggtct gatttttcat ccctatttac ttggtgagcg agcaccgctt
    16261 tgggacgcag atgcgtttgg cagttttatc ggtttacagc ggcaacatac agaaaaagaa
    16321 atgatgaagg cgattctaga agggatctgt ctcaaccttt atcggatttt aaaaggagtt
    16381 tgccaggaaa atcagctcac agagatccgt gctacaggcg ggtttgctca atctccgatt
    16441 tttcgtcaga tgatggctga tgtactgggt tacccgcttg ttttccctac tgtcacagaa
    16501 gcatccgcat taggagctgt gattttaggc tggagaagca tcggaaagat ccaaagtatc
    16561 agtgaagcac aggaattgat agaattgaaa gagcaagttg gagtacaaga aactgatcat
    16621 aaaaaatatc agaaaatcta tccattattt gtttcgacac aaaagcattt agcacagact
    16681 tatcagcaat ttgctcaact gcgagaagaa ttagctgatt ctgaaaaatg attggcttta
    16741 cggataaaaa agcgaactga aaatcgttct caggtaaatg attttgttgt acttttcatt
    16801 gaaagtctac tattatttta agtgtaagaa aatgttagga ggcaagaaag atgaaattcc
    16861 aacaaaccaa agaagtatta aatcaattag ttgcagatct aagccaaatg tctgttgttg
    16921 ttcaccaaac acattggtac atgagaggac caggtttcct tacattgcac ccaatgatgg
    16981 atgagttcat ggatgatttg aacgctcagt tagatgaagt atccgaacgc ttgatcacat
    17041 tagacggttc accttattct acattgcgtg aatttgcaga taacacaaaa atccctgatg
    17101 aaattggccg ctgggatcgt actattccag aacgtttgga aacacttgtt gcaggctatc
    17161 gttatctagc agatctttac caaaaaggaa tcgaagtttc tggagaagaa ggcgacgatc
    17221 caacacaaga tatctttatc gaattcaaaa cagccacaga aaaacgtatc tggatgatcc
    17281 aagctcattt gggcaaagcg ccagagatcg acgcgtaaaa actgttgttg aacttgaccg
    17341 aaaaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa cgtcaaaata
    17401 gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt
    17461 taccaagagg ttgtaagcct gccgttcagc tccgagtcac aagaaacaat tttaaaaaac
    17521 agcttcttat gttttatgga tattgtgact tgtgaccgag gagttggcag gagaacaccg
    17581 tttattcaaa ataaggggtt gtgacaagac ttttgtcaca gcctcttttt gtttttcatt
    17641 ttgacaggaa acgacccttt tatataaaag aaagaaaaga agtaaagttt catataaatt
    17701 tttcaatcaa gcagaaaaac gttgacattg actagattag cgggtaatat gttaaatggt
    17761 attgtttgcc ccacaaagag ccccggaaac tcgaagtgta tgtcaaacat cagaacttaa
    17821 attggaggaa aataacgtgc gtacaacata tatggccaaa ccaggcgaag tagaacgtaa
    17881 atggtatgta gtagacgcaa ctgatgttcc attgggacgt ctttcaacag ttgttgcatc
    17941 tgtactacgt ggtaaaaata aaccaacttt cacacctcat ttggatactg gcgatttcgt
    18001 catcgtcatc aatgcagaca aagtgaaatt aacaggtaaa aaagcgactg acaaaattta
    18061 ttaccgtcac agcaactacc caggaggatt gaaatcaatc actgctggcg aattgcgtgc
    18121 taaaaattct cgtcgtttga tcgaaacttc tgtaaaaggt atgcttccta aaaacacttt
    18181 aggccgcaaa caatttacaa aattgaacgt atacggtgga gcagaacatc cacatgcagc
    18241 acaacaacca gaagttttag acatcacaaa cttaatctaa taggagggaa tttcattggc
    18301 acaagttcaa tatatcggca caggccgtcg taaaaacgca gttgctcgcg tacgtctagt
    18361 accaggaact ggcaaaatta ctgtaaataa aaaagatgtt gaagaataca ttccacatgc
    18421 tgacttgcgt gaagtaatca atcaaccttt cgctgttact gaaacaaaag gcgcatatga
    18481 cgtatttgtt aacgtaaacg gcggaggata cgctggacaa tcaggagcta tccgtcatgg
    18541 tatcgctcgt gcattgctag aagttgatcc tgacttccgt ttagctctta aacgtgctgg
    18601 cttacttact cgtgacgcac gtatggtcga acgtaaaaaa ccaggtctca aaaaggctcg
    18661 taaagcatct caattctcta aacgttaata ttatttatat atcaatgttt caaggcactt
    18721 ttcaattgtt tgaaaagtgt ctttttcttg tttaagaaca catttaagaa cacatactaa
    18781 tcacatttat tcaatcctct ctgaaaggct tcaaaaacag ttttatctac tacatctggt
    18841 gtatttacgt atattctcgt tgtagaaata tctgagtgtg ttaaagcact agatatttga
    18901 ttcatatttg ctccgccttg tctagctata gttgcatagg tatgacgaag cttatgtgga
    18961 gataaatgag ccaattcagg atgtcgtctt ttaattgaat tgattcgata gtttaggtaa
    19021 tcagcgtgaa cagggcaatt tacttctccg gatggtttag tataggtaaa gagatactga
    19081 tctggtgttt gattaatttt taatctcaac aattgctcag cttgcactga tttccatttg
    19141 cttagtaatg ccattacaat ttcgggtact tgaattacgg tatcctttcg accttttgta
    19201 aatttctttt tttgatattt atccaaagca tgtttaaggc gaaccgtttg tttctcaaag
    19261 tcgatatatt tccattgcaa agcatacgtc tcactttttc tatcaccaag atatagggta
    19321 agcatgaaaa gagtatagtc atgaagagta agcaagtgat tattcaagtc agtgtttact
    19381 gcttctatcc aagcaataag ctcttctgac gataatgctt gattaccttc ccgtatcctt
    19441 ctttcttcta aagcaatttt tttaggtgca gtgatacatt gaataatttt ggtagttcgg
    19501 ttaaattcaa tataatttag tatttctgca atatcaaaca tagaacggac atatcctttg
    19561 ataattttta tatttgcgta ttctttcgat ttttttgtta attcatctga tattatctca
    19621 gtatttgaat ttagatagtt cattgcatac tcaccaaaca ttggaaggat gtgcaatcgg
    19681 aatatatctt tagtattact aattgttacg taacttggag ctctttttga tctagtagtc
    19741 tgacctagtt catatctagg aagccatttt gattcataga attccttaaa ttttattttt
    19801 ccttttaatt ctagtgaatt tgcattctcc tctcttaaaa ctgtttcaat tttccttttc
    19861 attgcatttt cggctttaat agccaattgc ctagttggaa atactttttg atacttttct
    19921 gatgtaattc cagtgatttc tctgaatttt tttggatacc tcagattagt tttatatttc
    19981 ccatttgaaa gttttgtagg caaaattcct cactccctta aaaagtaagg cagtatattt
    20041 cgctattctt agtatatgcg gcgagaagaa attttcaaat tgttttaaat gtattcataa
    20101 aatctttgat atcctctatg tcaaagcgaa ctataccgtc aattctgatt actggaaaat
    20161 tatatatacg aatccactta tctaaagtat tattatcaat tcctagaaac tcacaggttt
    20221 gttttttatt caagtagact ctcaatttta taaaatcatc tcctaaaaaa gattagaatt
    20281 tttgaattgc tttgggtaag cacataggaa tttcacctct ccccagttta cggcgagcct
    20341 tacggaagta tcattatttg ctgctattgt catggcattt tgttttccca gaacaaaaag
    20401 aaatctctaa gtatttgtcg ctaatcaggt catggcgtac ttgagtttct agctcgaata
    20461 gcagggatcg ttattcaaaa attgtattta attgtcaatg atcaaaaata ggttttttcc
    20521 tattcactat taataacgat aaacatgtat aaaaagacac actacgaaaa aagttaaatg
    20581 gagtttactg attttcatat ttgtatatgg aacagacata gtcttgaata atttttataa
    20641 atgttttgat gcttatcaac ttattcaacc cgctatccac tttaagtata atttttccat
    20701 acgccaataa agtagtttta ggagcatgtt ctcatttgag aacgctcctt tttt
//
LOCUS       DAZKCY010000042        20711 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000042 DAZKCY010000000
VERSION     DAZKCY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.042"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..569
                     /locus_tag="ACG8XC_001392"
     CDS             87..569
                     /locus_tag="ACG8XC_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191099.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            766..1656
                     /locus_tag="ACG8XC_001393"
     CDS             766..1656
                     /locus_tag="ACG8XC_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFP9191100.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWEIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1981..4455
                     /locus_tag="ACG8XC_001394"
     CDS             1981..4455
                     /locus_tag="ACG8XC_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9191101.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(4558..6294)
                     /locus_tag="ACG8XC_001395"
     CDS             complement(4558..6294)
                     /locus_tag="ACG8XC_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9191102.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6291..6995)
                     /locus_tag="ACG8XC_001396"
     CDS             complement(6291..6995)
                     /locus_tag="ACG8XC_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9191103.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7126..7628
                     /locus_tag="ACG8XC_001397"
                     /pseudo
     CDS             7126..7628
                     /locus_tag="ACG8XC_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
     gene            7588..7926
                     /locus_tag="ACG8XC_001398"
     CDS             7588..7926
                     /locus_tag="ACG8XC_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFP9191104.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7947..9365
                     /gene="ffh"
                     /locus_tag="ACG8XC_001399"
     CDS             7947..9365
                     /gene="ffh"
                     /locus_tag="ACG8XC_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP9191105.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9477..10055
                     /locus_tag="ACG8XC_001400"
     CDS             9477..10055
                     /locus_tag="ACG8XC_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP9191106.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10059..10580
                     /locus_tag="ACG8XC_001401"
     CDS             10059..10580
                     /locus_tag="ACG8XC_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFP9191107.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10659..11213)
                     /locus_tag="ACG8XC_001402"
     CDS             complement(10659..11213)
                     /locus_tag="ACG8XC_001402"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9191108.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11466..11741
                     /gene="rpsP"
                     /locus_tag="ACG8XC_001403"
     CDS             11466..11741
                     /gene="rpsP"
                     /locus_tag="ACG8XC_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP9191109.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11753..12004
                     /locus_tag="ACG8XC_001404"
     CDS             11753..12004
                     /locus_tag="ACG8XC_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFP9191110.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12128..12919
                     /locus_tag="ACG8XC_001405"
     CDS             12128..12919
                     /locus_tag="ACG8XC_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191111.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNDKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13089..13610
                     /gene="rimM"
                     /locus_tag="ACG8XC_001406"
     CDS             13089..13610
                     /gene="rimM"
                     /locus_tag="ACG8XC_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP9191112.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13600..14361
                     /gene="trmD"
                     /locus_tag="ACG8XC_001407"
     CDS             13600..14361
                     /gene="trmD"
                     /locus_tag="ACG8XC_001407"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP9191113.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14497..14844
                     /gene="rplS"
                     /locus_tag="ACG8XC_001408"
     CDS             14497..14844
                     /gene="rplS"
                     /locus_tag="ACG8XC_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP9191114.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(14976..16112)
                     /locus_tag="ACG8XC_001409"
     CDS             complement(14976..16112)
                     /locus_tag="ACG8XC_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9191115.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPNLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKMPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVDFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPKGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(16229..16921)
                     /locus_tag="ACG8XC_001410"
     CDS             complement(16229..16921)
                     /locus_tag="ACG8XC_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191116.1"
                     /translation="MKKVILGLVFGAFFLTGCSQSNVVDNTGKTNEKDSSVTLSESTT
                     QSSKNSQEVDGPLLKVGQWTKEKRDTSGKIELIGTASPQSDIPLGDIGIYISNIKLLK
                     YSNYADPSAAGYYDANNHKDSEGNFYGLQIAFKVKNNSEVDYGYNGLAHAILDNGQQI
                     DFSSDDLMHTMTTNSFFKKTESKEFYSLAYLDPSKVNDITKVTIKTGVLYNPSDYSTV
                     AESAESTFNINR"
     gene            complement(17059..17712)
                     /locus_tag="ACG8XC_001411"
     CDS             complement(17059..17712)
                     /locus_tag="ACG8XC_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191117.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKVSKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     GQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(17762..18190)
                     /locus_tag="ACG8XC_001412"
     CDS             complement(17762..18190)
                     /locus_tag="ACG8XC_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9191118.1"
                     /translation="MEMDTINLVEELKRKYRSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIDELKNLYGLPESAYRFLI"
     gene            complement(18195..18569)
                     /locus_tag="ACG8XC_001413"
     CDS             complement(18195..18569)
                     /locus_tag="ACG8XC_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9191119.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            18854..19039
                     /locus_tag="ACG8XC_001414"
     CDS             18854..19039
                     /locus_tag="ACG8XC_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9191120.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            19052..19300
                     /locus_tag="ACG8XC_001415"
     CDS             19052..19300
                     /locus_tag="ACG8XC_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191121.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKITPKEFIEKHLVDL"
     gene            19331..19510
                     /locus_tag="ACG8XC_001416"
     CDS             19331..19510
                     /locus_tag="ACG8XC_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191122.1"
                     /translation="MGKFNRALVFSTPFIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(19553..20023)
                     /locus_tag="ACG8XC_001417"
     CDS             complement(19553..20023)
                     /locus_tag="ACG8XC_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191123.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            20110..>20711
                     /locus_tag="ACG8XC_001418"
     CDS             20110..>20711
                     /locus_tag="ACG8XC_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP9191124.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAV"
BASE COUNT         6719 a         3636 c         4099 g         6257 t
ORIGIN      
        1 tctaaaaaaa aagtttttcc ccctaatttt tttagataga aaattgtata gtatgatgag
       61 agaaaaagta gtttggggga acgttaatga gaagaaaagc agtaatcgat cggctttatt
      121 tatttttttg gttttttcct atcgttttgc taccttttac atgggtaaat gctgttgtac
      181 aaggtgcgga gatattaggc ctgatccaag gaagcggatt tgtggtttct aggaattttc
      241 cagctttgac tgttttctat ttagtgataa gtgtaggtct cttattacca aaatctctga
      301 tcaatcacta caattggcag ctgctttact tttcttgctt attagtattt acatctatgt
      361 tttctttgct gcctcgcttg attatcggcc ctaatggttt gagcgagagt ggattgaagg
      421 attcagctta ttcctccatg tatttcttga cgatcaaacc atggttctat ttagtaattg
      481 cctcatttat tcttgcaaca tttttttact tctggacaga aatcaggata aaaagtgaac
      541 gatctgtatc tataaaaaat aactattgat ggtggatcat cagtaaaaaa gcatatgagc
      601 tgcgtaaaat gcatgtttct catatgcttt ttttattatt atgtaaagaa tgcgaaaaac
      661 gtttacatga tttttacata acttaactat atttttacag caaccatacg agggatttac
      721 agtcgactga taaactttag ttgtaaataa gaaaggagca ataaaatgaa aaaagtattg
      781 tttactctag gtatgatggg aattctttta gccggatgcg gctcaggatc aggaaataca
      841 gatggctctg ccacacaaaa taattcaagt gactcaaatg aacaagtaaa aatcgttgcg
      901 gtaggttcga cagcattaca gccgttagtc gatgcagcac aagaaagctt tgtacaagaa
      961 aatccaaatt atcaaatctc tgtacaagga ggaggaagcg gaactggttt gagccaagtc
     1021 gaagctggag cagttactat cggaaactct gatgtatttg cagaagaaag agacggagtt
     1081 gatgcttcaa aattagtgga tcataaagta gcagtggtcg ggatggcacc aatcgtcaat
     1141 aaagatacag atgtcaaaga tatcaccaaa caggaattga ttgatatctt tacaggaaaa
     1201 atcactaact ggaaggaagt tggcgggaaa gatcaaaaaa tcaacgtcgt gaaccgtgca
     1261 aacggcagcg gtacccgcgc gacattcgaa aaatggggac tggatggtgc tacccctgtg
     1321 cagtcccaag aacaagattc atcaggaaca gttcggcagc tggtaagtca aacaccagga
     1381 gcaatcagct atttagcttt ctcatatctc gatgattcca cacaagcact aagtatcgac
     1441 ggtgtagaac caaaagaaga gaatgtagca gataacagct gggaaatctg gtcatatgaa
     1501 catatgtaca cgaacggaaa accttcccct gaagtccaaa aattcttaga ctacatgatg
     1561 acagaagaaa tccaagaagg acctgtcaaa gaattgggct atttaccaat cacaatgatg
     1621 gaagtagaac gtgatcacga aggaaatatc aaataactag cagataaaca tgccacggta
     1681 gtcgttacta ttaaggaaaa atcaactatg tttccccaca tatacatttt tcctttaatt
     1741 gtgaaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt
     1801 gaacaccgtt tattcgaaaa aaagagctac gacatagaac tgtcgtagct tttttttgct
     1861 atatgacagg aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc catatctgtt
     1921 ctatactgga taggagaagt ttggaaatat ccagaaaacg aaatcaatcg aggtagccac
     1981 atgaagaata aatggataaa tcgaagtaaa aaaaagaaag taacacaaag atcatcagat
     2041 aaagtcatcc gtctttttcc atcgtataaa gaaggattaa cggcagatca agtcaaggaa
     2101 cgaatagaaa aaggtgcagc aaataattca gtcgatccca cattcaaaac aaatcagcaa
     2161 atcgtattgg aaaacatatt tacgtatttc aatttgatct ttcttatctt agccattttg
     2221 ctttgtttag tagaatccta taagaacttg acttttttac cagttatcat agccaataca
     2281 ggaatcgcca tttttcagga aattcgatcc aaaaagatct tggacgaact aaatgtttta
     2341 aatacagcaa aagtcaatgt catacgagac gggatagaac aaacaataga tatagaagac
     2401 cttgtaatag atgacattgt tattttagag actggacatc aaattccagc agatgcagag
     2461 gtcgtggaag ggaaactgca agttaatgag gctttattga caggcgaagc agatgaaata
     2521 accaaagaaa taggcgatcc attaatgtcc ggcagtttca ttgtttccgg aaaagcttac
     2581 gcccgattag acaaagttgg agcagattcg tatatttccc agctgacagt caaagccaag
     2641 acaatgggag aaggagaaca gtcggagatg attgcttctc ttaatcaatt gatcaaatgg
     2701 attgggatca tcattattcc tatcggtatc ctgctttttt ctcaaagtta cttttttaat
     2761 ggaaacacga tcaaagaaag tgtcgttgca atggaagcag cattgatcgg tatgatacct
     2821 gaaggactgt acttgctgac gacgattgca ttagcattaa gtgcgactaa actagcaaag
     2881 caaagagttc ttttacacaa tatgaaaagc attgagacat tggcaagggt aaatgtcctt
     2941 tgtgtagata aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa agtgatcgtc
     3001 tctaaaaaac aagaaataat cagtcagtca gatcaattgg aagcatgcat tgctgactat
     3061 gcaaaagcga tggcaggaga caatgcaaca atggaagctg ttaaagcata ttttacaaaa
     3121 accactgatc aatcctatca ccatgttatt cctttttctt ctgtccaaaa attcagcagt
     3181 gtagcgttgg cagacaaggt gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa
     3241 ttgccggaat atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc
     3301 tttggtgtgt acgatgggat tcttgaagaa ccagtattga cagaagcagt cacaccatta
     3361 ggctatattc tgattgctaa tcctatcaga aaagaagcaa gagccacatt tgattacttc
     3421 aaaaaacagc aagtagccat caaagtgatt tctggagata atccgctcac cgtatccaat
     3481 gtagctgtcc aagcagggat ttcacatgct gatcagtacg ttgatgcatc taaattatca
     3541 gaagaagaat atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa
     3601 caaaagaaaa tcttcgttca attgctaaag cgtcgtaaca atacagtagc tatgacaggt
     3661 gatggcgtga atgatattct agccatgaaa gaagcagact gcagtatagc gatggcttcc
     3721 ggaaatgaag ccgccatgca ggcctcacaa gtggtcttac ttgattctga cttttcacgc
     3781 atgccagaag ttgttgcaga aggacggcgc gttgtgaaca atattgagcg atcagccagc
     3841 ttgtttttag tgaagaatat tttctccttt ttgctctcac tgttttcagt gatctttgct
     3901 ctgacctatc ctttagaacc atcacaaatc acattgatta gtttgtttac tatcggactt
     3961 ccatctttct tgcttgctct agaagaaaac aaaaagcgaa tcagagggaa attcatcatg
     4021 aatgtgatgg aaaaagctgt gccaggtgga ctaacagata tgatcgtagt cggtgcgttg
     4081 gtgatctgcg gggtgacttt gaatctaaat aaaacagatg tttctacagc atctaccatg
     4141 cttttgattg cagtagggtt cctcgttctc tataaaatct gttcaccgct gaataaattc
     4201 agaagtcaga tcattctttt ctgtgctagc ggtaactttt tttcggtcat ctttttgcat
     4261 aagctgtttt caatcaccgg tatctcagct gtatcacttt tgctgttgag cattctattt
     4321 ttctcggcag attcgatttt taggcatttg accacattgg tagaatattt gtttgaacgt
     4381 aaaaatgaaa aagagcaaaa gccgtttagt cgcgtagtta aaaaatttct atcgatattt
     4441 aaacgagaaa attgacgaaa agtatctcaa aaaagagtaa aaaagaacaa ccactcgtgt
     4501 atctttcgaa gcaggatgag tcatccaact ttaaaacgca cgagcagttg ctctttttta
     4561 cattttcgga atggtaacgg tgaaagtcga acctgttcca agatgactgt ctacagtaat
     4621 cgtaccatga aggagttgca cgtagtcttt tacaatcgct aaccctaatc cggtcccgcc
     4681 tgaatgccgg cttcgcgcct tgtctacgcg gtaaaaacgc tcaaagatcc gttcctgatc
     4741 ctcaatgtcg atacctatac caaagtcttg gacagaaaac gatagatcat ctgttgcctg
     4801 atagcggata ataatctcgc tgtcgctttt tgaatattgg acagcatttt caatcagatt
     4861 tttgataatt ggataaagga tatctgtttt agtagtaaat ggttcgttct ttccaatcaa
     4921 tcgaattgtc aaccgtttct tttcgatgat tggctgataa ttttgaacga tttgctggaa
     4981 ataaggttct gtataaattg tctggatctc gtaagaattc tcgccatctt ttgacagctg
     5041 gatgatttcc cgaatcagtt tatctaaccg gatcgcatct ttttgcatga tctcaagaaa
     5101 agaagtcaga gtttgcgggt catcttttgc accatccagt aaagtctcgg taaaaccaat
     5161 cagtgaagtc acaggagttt ttaattcgtg agagacgttg ccaacgaaat ccttttgtag
     5221 cttttctaaa cgtcgcactt tcgttaagtc atacgccacc cctagaatct gtccggtacc
     5281 ttctgaatta gtaaagtagc gcaaactcaa gtctaatgtc tgccgacctt ctgtaagggt
     5341 gatctcctga tggatcagcg gatgctcagg agtcacttga tgaatcagct gaatgagttt
     5401 tggctcttga atgacttctg tgtaccgctg ttcaggttga tagcttacca cgtttaaatg
     5461 tagctgcatc tttggattaa gcagtagtaa acgagaatca gcatcatcaa tcaggaatac
     5521 gccaatcatc agttcgttca gtaacgtata taattgctct tctgtggtag tatacgcccg
     5581 atacatctta ctcatttgtt cgctcaacgc attgatcgtc tgatacaatt cttcccattg
     5641 atcgggtgtt tgcatgatta cttccgcctg attaggattt tgcaccattt tttttagtac
     5701 gggcagcacg gttttcaatg gctggttttt ttgatagatc agataataga tcattcctgc
     5761 aagaacaagg aagaaagaca gaaagaagaa aaagacccaa cggcgaaaac tctcagttct
     5821 cggtaaaaaa ccgcttgtcg gttctgcgat acgtaaaatg gcttctaact ggccactttt
     5881 tttgactggt aatgcgacat ataaaagatc cccattcaaa gtagtacttt tgcgcaaagc
     5941 gctacctagg ttcccaccat tcaatacggc tttgatttcg ggacgggagt ttcgctgttc
     6001 attcaatgtt tcattgtttg tgtcaaataa gatctttcca gtagcatcca ttaaagtaat
     6061 ccgttcgttc gactgatgga cgaattcttc caaagcagtt ttgttttgcg ctgcttggag
     6121 gttttcgaca tctaattgcc ggatcagcag ttgtgctttt tttgtcaggt agctttcttg
     6181 ctgttccacg atctgctgtt gaaaataatg ggaaatcaat tgccagccac caaaaaacaa
     6241 actaactaaa agaataagaa agaagagacg ttcataaatt tttttccatt tcatcgtttg
     6301 ggttcctgaa acttatagcc aaatcctcta atagtcacca agtactttgg gtgttttgga
     6361 tctcgctcga ttttttctcg caaatgacta acgtgtacat ctacgattcg gctttgtcct
     6421 gcgaagtcaa aattccaaat gcggtctagc aatgtgtctc ggtcgatcac acggtctttt
     6481 cgtttcatga aataaacaag taattcaaat tctttcggtg ttagttcaat cagatggtcg
     6541 tctacggtca cttgataatt ggtcaaatca gcagtgattt gtccaatcga tagatattca
     6601 ggctcagctt catcttttga ttgcttacga ggttcaattc gtctgaatat tgctttcata
     6661 cgggctaata cttcgcgagg actaaatggt ttggtcaaat agtcgtcagc acctatttct
     6721 aggccaatga tccgatccac ttggtcatct ttagccgtca gcatcaaaat cggtgtttca
     6781 tttttttctt gacgcagccg ctgagtgatc tccatgccat ccatcgttgg aagcatcaca
     6841 tctaaaatga tgaaatcaaa tgattgttct aatgctaatt ctaatccttc gcctccatct
     6901 gtggcactgg ttaccttgta gccttctttt tccaaattga acgtcaagag cgttacaata
     6961 gaaggttcat catcaacaac tagtattttt ttcattcaaa tctcctttaa ataggtttca
     7021 ttctgtttat attttagcat agaatcagta caaagggcta ttttacaaaa gaaataaatg
     7081 gtataatagg aaggcacttt gctgaataga aaggaagagc agtcaatgat tggaatcagt
     7141 gcatgtttag gcggtctggc ctgtcgctat gatggacaga aaaaagaagt accagaattg
     7201 atggaactag ttgaaactgg agaagctgtc atggtctgtc cagaagtgat cggcggatta
     7261 ccaattccta gagatcccgc tgaaatcatt ggtggaaatg gattcgatgt gtggaaagat
     7321 caagcaaaag tatggacgac aagcggcgaa gatgtaacgg aagcgtacaa aaatggcgcg
     7381 atcaaagcct atcaaaaatt gcaggaaaaa cacattgatc tagtcatcat gaaggaaaaa
     7441 agcccttcat gcggaaccca ttcgatttat gacgggagtt tttccggaat aaaaaaaatg
     7501 gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc tctgatgaag
     7561 aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg
     7621 ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat ggaattatat
     7681 tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag ccgacaagca
     7741 gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa aaaactccat
     7801 cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc atatattaca
     7861 gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca agcaatcgaa
     7921 gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa cgtctccagc
     7981 aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta aaagaaatga
     8041 tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta gtcaaagatt
     8101 ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt ttatcacctg
     8161 cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt tctgaaacag
     8221 cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt cttcaagggg
     8281 caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat gaaaatgctc
     8341 gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat
     8401 taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc ccggtagaga
     8461 tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata
     8521 cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa atcaaagaat
     8581 tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa gatgccgtca
     8641 acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc actaaacttg
     8701 atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc gcaccaatca
     8761 aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca gatcgtatgg
     8821 ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg cagcaagact
     8881 acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca ttcgatttca
     8941 atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa gatctaatca
     9001 aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag
     9061 atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga gcaaatcctg
     9121 atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg
     9181 aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag caaatgtcca
     9241 aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac
     9301 taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga
     9361 aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg gagcttttct
     9421 atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg gcacagatga
     9481 gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact gtctggggtg
     9541 ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt aatcaagccg
     9601 gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca tatgatcgat
     9661 tcgatatcaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta atggaaaaca
     9721 aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc aaagcagtct
     9781 tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt tctaatggaa
     9841 aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt cctctttcag
     9901 atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc acaacaatct
     9961 atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt ttccgcaaag
    10021 aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc atattttatg
    10081 gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa tggctttttg
    10141 acactccaac aggtaagcaa accacttatg aagcttttta tgaaacaatc gatacgacca
    10201 tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa gcaatttatc
    10261 cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca gatgctgagg
    10321 tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa ggaaaagata
    10381 tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa atgattgatg
    10441 aatggtatat ccagattacc cctgtattat taggggacgg gattccttta ttccaaaaag
    10501 gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa tttatcgaat
    10561 tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat gcagaaatgt
    10621 cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa tttgttagaa
    10681 aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc aacgatatta
    10741 ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg ctgcaaggtt
    10801 ggcacattca aaaagacaag tgattggcgt agatggtggt ttgcgccaaa gccgccgatc
    10861 ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact gtgtccataa
    10921 ggccttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg gttgtattca
    10981 ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg agtccattca
    11041 gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc atcgattttt
    11101 acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc tacttttttg
    11161 ttgtaagaat cttttcttaa actaccaata aaaaatccaa ttttttttgt catacgattt
    11221 ttcctccttc aaactatcta taacaatctt acaaaaaata agtaactgga gcaaataaaa
    11281 acacttactg acaaaagcta catagaagat agattactta tttttaacaa aaaagaagtc
    11341 taaaatcttc tatttcctaa aaaaaatcga atttaatcaa gctgtaaaga aaaaaagctt
    11401 tacagggtaa aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg aggtggaaat
    11461 aaataatggc agttaaaatc cgtttaaaac gtatgggttc taaaaagagt ccgttttacc
    11521 gtatcgtcgt agctgattca cgttctcctc gtgatggacg tttcatcgaa actgtaggta
    11581 cttacaatcc tttgaaagac cctgcagaag tagttttaaa agaagactta gttcttgact
    11641 ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa catcctttca aaagaaggcg
    11701 ttatgcaaaa acaccacgaa gctaaattct caaagaaata aggtgaccgg atatgaaaga
    11761 tttaagcgac ttagtcttaa caatcgttcg tccgctagtc acttatcctg atcaagtcaa
    11821 gttagacgtc gtagagtcga aagactttta cgagtacaat ctaacagtgg ctcctgaaga
    11881 tattggccgt atcattggaa aacaagggcg tgttgctaaa gcaatccgca caatcgttta
    11941 cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg ataaggaaaa
    12001 gtaaataaac gtgctgtact cgaattttcg agtgcagctt ttttgctttt ttattaattg
    12061 aagaaatgat tatttggcaa aataagcaag aaagcgtatc cttataggag gagggagtga
    12121 acagccaatg agaaagaaaa tgttatttat tccatttgct ctattgttaa gcgcttgtag
    12181 ttcggttact caagaatcag acaatacaca tctatcaagc agtatatctg aaactgttac
    12241 cagtccagaa aaaagtagta ccaaaacggc taccaccact gaacaaacaa tggctacctc
    12301 aaataatgat aaaaagactg ctttagacca gttgaaagaa caacagccaa atgttcccat
    12361 gcctctagat gtaccagtct cttcaggcta tctcaatatc gcagcgacac atacaaagca
    12421 aggttattct attttatatt acagaacaga tcgtcccctc gggttgaatg ctgacgaatt
    12481 gaatcaagaa acgcctattg caacctatct atatcaatat gggtttgctt ctagtcaaga
    12541 aacaatccaa gttttgcagc cctttgagat cgacacaaat ggtcagcaag tggacttagg
    12601 ttctcgaatc acagggtatc agcaaggtgc agcaggttca agttttcttg aatggcaaga
    12661 aggaaactgg cgtattcgaa tccgtgggaa taatatagaa ggccaagatc ccttactatt
    12721 agctaaagaa atcgtagctt atttggaaga aaacagtcta ccagcacctg aacaatttgg
    12781 taaaattact gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga
    12841 accgaaaaat gcctatacga tcactcatca agatcctatg tctgcgttga agatggcagt
    12901 ttctatgaag aggttgtgaa atcagcgcgc tgatctgagc ccatcattta actccaagtc
    12961 acaggaagta atcaaaaata gcacgccgca tttttgcata attttggctt aataccttaa
    13021 aagaaaacta gaattgcctc ttcacacatg ctaaaataaa acagacaaaa aagaaatggg
    13081 gatataagtt gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag
    13141 gcgaagtccg tgttatctca acaacggatt tcccagaaga acgttacaaa aaaggaacgg
    13201 tgttaacact attccaagac ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca
    13261 aacataaaaa ttttgactta ttgagttttg aaggacatcc ttctatcaat gatgtggaaa
    13321 aatatcggga tggcatatta aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg
    13381 aattctatta ccatgagatc atcggtctgc atgtattaga agatggaact gagttaggta
    13441 aagtaaaaga aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa
    13501 aagctgatat attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg
    13561 gagtggtccg tgtagaaatt ccagaaggac tgattgacga tgaagattga tgtattgact
    13621 ttatttccaa gaatgtttga aggtcctatg ggggaatcca tcatcggtaa ggctgtagat
    13681 aaaggattat tagatatcaa tatctctaac ttccgcgatt attcagataa caaacatcag
    13741 acagtagatg attatccata cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc
    13801 tatgataaca tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt
    13861 cttgacccag ctggtaaacg attcgatcag aaaatggcag aagaattttc acaagaagaa
    13921 cacttagtat tcatttgtgg tcattatgag ggatatgatg aacgtatccg cacattagtg
    13981 acagatgaag tttccttagg agattatgtc cttactgggg gagaattagg tgcgatggtc
    14041 atgatcgatg caacagttcg gcttttgcca gatgtgttag ggaatcagac ttcagctcaa
    14101 acggattctt attcaaccgg tttattggaa catccccaat atactcgccc ggcagaatat
    14161 aaagggatga aagtaccaga agtattgaca aatggaaatc ataaattgat tgaagaatgg
    14221 caattaaaag aatcactaag aaggacctat caacgtcggc ctgatctttt ggaaacactt
    14281 gaatggacac ctcaaatgac gaaattttta gaagaaatca agaaagaaga acaagaaaaa
    14341 acagcagatg aagccaagtg actttttcta aaatattctt tacagtaggt tgataatatg
    14401 ttagtatttt atggtgagtg caaaagcact taactattac gatattccgc tgtgagaaaa
    14461 gctcataaga atatttggaa taaggagaag aaaaccatga atccattaat cgaagaatta
    14521 acaaaagaac aattacgttc tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt
    14581 cacgcaaaag ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg tgttgttatc
    14641 aaacgccgcg gtgctggaat cagcgaaact tatacagtac gtaaagtttc taacggagtg
    14701 ggcgttgaac gtacattccc attgcataca ccacgtgttg caaaaatcga agtagttcgc
    14761 tacggtaaag tacgtcgtgc aaaattgtac tacctacgtg cattacacgg aaaagctgct
    14821 cgtatcaaag aaatccgtcg ttaatcattg ttatcagact gagaaagaac ccttggtatt
    14881 caagggttct ttttttatta tctgtttttt tatattaacg atttctattt ggtacatttt
    14941 tgattaataa aaaggggcaa aaaaggggca acaagttaca gtttttctat atcttctttc
    15001 aatttttctt ttactccttt tgtcacatgc aaataaatct tttcagtgat atcactattt
    15061 tcgtgcccaa cgcgatcctg aatagcatac agaggagttc ctagttccgc taattttgaa
    15121 atatgggtat gtctaaaaat atgtgaacta agttttttat caatcttcat gtcagcttta
    15181 tgatttctca aataggtgtt aattgctgtt agttggaaag gagttccttt cgttgtttga
    15241 aatagaaatt gacccttcgg atttaattct agaagctcat tgtaaatagc tatcgctttt
    15301 tttggtaaat caatttctcg cattccagca gcagtttttg tagaatcaga ttttttcata
    15361 tcagctattg aacgttctct atacatcatc gtcccattta taactactga tgcattatta
    15421 ttggtaatgt gtacatcgtc tttacttaat gcgattgctt ctccaggtct cattccagtc
    15481 aaatatagcc actggaaaag gagagaatat cttttattgt gtgaagttgt gaaatctact
    15541 agtcttttat attcatcatc ttctaaaaac ttatctttaa tttttatcgt ttttgactct
    15601 cgtttataat caataactac ttcgttgata ggattttttt ctacatagcc ttttttcata
    15661 gcgtatgaaa aaagaagatt caatttggat ttaattacac tgacgtactt atttgacaag
    15721 tcatctttat atatcatatc ttcaaaaata ttatttaaat ctactgtatt aataccagaa
    15781 acgatgtaag tctcaggcat cttcttcttt atagtgttta agatattatt cgtaggataa
    15841 tatgtagact ccttaacttg ccttttgtaa ataactaacc attcttcgac aagctcatga
    15901 aatgttagat taggcttctg gagtgttttt tcatttagtt ttatatcaat ttttttattt
    15961 aactccagta atgccacttt ctgagtttct cgagacttat ttttataagt aatgctcact
    16021 ttttttcttt ttctagtttt tggatcaata tatcgttcat tatatttata tacttttttt
    16081 ccgtttttat cagttttggt ttcaatccac attgttttca tctcctattt gttgctataa
    16141 taggaatgga taagtaagcc gattatagca ggtttatttt tcatcacgtc cacaaacttt
    16201 ggcgagggag ggggcgtgtt tttgtatttt aacgatttat attgaatgtt gattcagcac
    16261 tttcagcaac tgtactataa tcagacggat tatataacac tccggttttt atcgttactt
    16321 ttgtaatatc gttaacttta gatggatcta agtaagctaa actataaaat tctttacttt
    16381 cagttttttt gaaaaacgag tttgttgtca ttgtatgcat aagatcgtct gaactgaaat
    16441 caatttgttg gccgttatca agtatggcgt gtgcaagccc attataacca taatctactt
    16501 cagaattgtt tttaactttg aatgcaattt gtaaaccata aaaatttcct tcactatctt
    16561 tgtgattatt tgcatcgtag taaccagcag cgctagggtc agcataattg gaatacttga
    16621 gtagtttgat attagatata taaatgccga tatcaccaag aggaatatct gattgtgggc
    16681 ttgccgttcc aattaattca attttgccac ttgtatctct tttttctttg gtccattgac
    16741 caactttgag gagggggcca tcaacctcct gtgaattttt agaagactgt gttgtacttt
    16801 ctgataacgt aactgaagag tctttttcgt ttgttttgcc agtattatct acaacattag
    16861 attggctaca tccggtaaga aaaaaagcac caaatactaa ccctaaaatc acttttttca
    16921 ttctttattc tccgtttcta tgatattatt tttatgtagg atcttagaaa cgagatctta
    16981 gtccgtgttg cagcacgggc ttttttcttt ataacttttt tagagattat aggcaaaata
    17041 gtaggacaca aaaatatatt attgtattcc gtatttagaa aatcctaatt gaacttcacc
    17101 agaagttttt tgctgtgttg tacgcaatgc ttcttcttca gacattccat tctgtacttt
    17161 ccatgcaaca ggcgacatcc cgtatttgtt aacaaaatca gtaagtgata aagtgtcagc
    17221 gtcttgctga ccgcttgttt gttggtcttc tggattttgt tgagatgctg cttgttgttc
    17281 tttctgatct tgactgataa tattgccagc atcatctgta gtcaatccat tttcataaag
    17341 ggccacgccg aaagcttccc actctttgtt ggaccaattt gcacgatcag ctggagttga
    17401 ctgtaaagtg cgttgtttca tctgttcata tgtttcttct tgaggtgcgg tttggattgt
    17461 atcctgactg gagcttataa ctgttgggct aggttccgcc gtagcttggt tagagcttgt
    17521 aactgttgaa ctagaatctg tcttagatgt agatttgcta gtagaggaac tggtttcagt
    17581 tgtttctttt gttttactta cttttgtttc ttggttagaa gtggcatctg ttgattcagc
    17641 ttttttatta tttgaacaag ctgaaagtag cagagcagta cttaacaaca acataacgct
    17701 aacttttttc attttataat tcctctttct cgttgtaata tgtgtgctaa cacgggcttt
    17761 tttatataag aaaacgataa gcgctttctg gaagtccgta aagattcttt aattcatcga
    17821 tttttttggg atattgatca ttatcttctt tataaagaga aacaatgaga ttagcagcaa
    17881 agcaattagc ttcgctttca gatttgcttc tagatgttct tgttgataca tagtaactgg
    17941 ataagccgcg atgaaaaata gcgtgaccta attcgtgagc gcaaatgtag aatctttcct
    18001 cagagtctcg cagttcatca tttaagaaga ttattgcacg acctctaatt tcttgaaact
    18061 gtcctttggg attttcgata aaaggaacgt attgaatttt aatgcccatc ttttcacaaa
    18121 tataaaaagg attagcagac cggtatttcc gcttcaactc ctcgactaaa ttaatcgtat
    18181 ccatctccat aagctcacat ctttttgcct ttttctttgt cttctttcac aatatcccag
    18241 aaggttccaa taaggatatc ttttacgcgt tggacctgtt caggcgtcag tgtttctccg
    18301 ccataagaca tattgacatt tgaatctagt aatttatcaa gttcaactac ttcatctttt
    18361 gttgcccagt caggaacttg gtttctaccg agtaaataat cagtagttac attaaaataa
    18421 tctgctacaa gttgtagtct tttagtacta ggagttctat ttttccattg ataaatcgtg
    18481 tttttaggta tatttaattc ttcttccaat tgagttatac ttatgcctct tttatgagct
    18541 aattccttta ttctatctag taaattcatt ttcgcacctc acaagctacg aaaacactaa
    18601 taaaaaaatt agttaaaggt gttgacaact aataaaattg ttagtatact gttttcgtaa
    18661 gctaaaatat tagctaaaaa gacttgataa ctaataaaca cttcacggtc ggcaaactct
    18721 gaaatgtaaa ttactaggca tttctgtgtc ttatttagct atatctatat actaataaaa
    18781 atattagtta ttgtcaacga atattagcta attttttagc ttacagacta ttttttagaa
    18841 aggagctatt tttatgtctg agaatttaga cttaaaaatt cgagcggaga tgagaaaaag
    18901 aagaatgact ttcaaagaac tagctgcgct tgttggtatt tcaggagctt atttatcaga
    18961 tattctaaac ggcaatcgtg atggaaagaa agcacaacag catatcgaaa cagtgaaaaa
    19021 aatattggac atccgatagg gggggtaggc gatgacaaaa ctaaaaaaac aagattttgt
    19081 aaaaaaatac aattattctc catctactta tcaacgtcga atgtcggaac taaaaaatac
    19141 agcaattttc tcagcggcgt atgaacgggt cacaggacaa gaagtttgga tcaatacaga
    19201 attatacgat aaatttttgt ctttcaaatc ctataacagg ttacgcacaa gaaagataac
    19261 acctaaagaa tttatcgaga agcatttagt tgatttataa aaaacagaac attttgagag
    19321 gtgaaggtta atgggcaaat tcaacagagc actagtattc agcacaccat tcatcatcta
    19381 cgctttaggc ctctggggaa gcaggcaagc gttgatagga acgattgttt acatggtttg
    19441 gatttttatg gggcttgatg aagctgaagc tgagtacaga gcgaaaaagc caaccgaggg
    19501 ggctgactaa aaatgaatag aaaagagaaa ctagaatgta tattactatt actcagttta
    19561 attctttcac taatttctct attgggaagt ttgtattttt gatatcaaca attttgtgga
    19621 tttgggttct atttagagta aatatcattt ttgtttggtg attaaggagt ttattaaaat
    19681 taattttccc tacttcaaat gcaaggtaac aagatatagc acttttagga ggaatattta
    19741 gcggtacagc ttcagagtat attctattat catcgactga tcctccttta gcaattaatt
    19801 tcttaaatgg agtagctgac catctgcggt ttaagttagc ggatgaaagt tctaaattaa
    19861 ctaacatagc tggttctgtt gaatagttag aaatgattac ttttgtataa atcatatctg
    19921 cagcgaagta agaagcatta agttcaactt ctagttgagg gcgcattcct tttattttta
    19981 tccctgttaa aaacgtactc aatataaaac ttattattga catccattga aaaattgtta
    20041 agtgaaaaaa attcaaaata aacaccacca gtttttaact aaattatacc aaaaaggaga
    20101 gaagaaataa tgcaagaatt agtaattttg aaaaataaag aagctgtgac tacgagctta
    20161 caagtggcag aaagcttcga gaaaaaacat caacatgttt taagagatat tgatgcacta
    20221 aaaaaagatg tgtccaattt tggacagatg tttgtagaag gtaatgaacc agattcatat
    20281 ggcagaaatc gacgagtttt cttcattagt agagatggat ttttcttgct ggctatgggg
    20341 tttacaggaa agaaagctat ctacttcaaa caaaaataca ttgaagcatt caacgaaatg
    20401 gaagatgtta ttcgcaagaa tactgttcct caaacaattg aagatatgat gatctatcaa
    20461 ttagaagaaa tgaaagatgt taaaaaagat gtttccatgc ttaaagatac tatgcgaatt
    20521 agcggacaac aagagtttga aattaagcaa aaaggaaata tgaaagttat gggagttcta
    20581 gggggaaaag aaagccgagc ttatgaagaa atcagcaaaa aagtattctc aaaattttgg
    20641 tctgaattta aacgtacctt ttcaatccca agatatggcg agttacctcg taagagattc
    20701 gatgatgctg t
//
LOCUS       DAZKCY010000043        19996 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000043 DAZKCY010000000
VERSION     DAZKCY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.043"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACG8XC_001419"
     CDS             4..816
                     /locus_tag="ACG8XC_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFP9191125.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACG8XC_001420"
     CDS             813..1613
                     /locus_tag="ACG8XC_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9191126.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8XC_001421"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8XC_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFP9191127.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACG8XC_001422"
     CDS             3110..4819
                     /locus_tag="ACG8XC_001422"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP9191128.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="ACG8XC_001423"
     CDS             5031..9383
                     /locus_tag="ACG8XC_001423"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFP9191129.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="ACG8XC_001424"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="ACG8XC_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP9191130.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="ACG8XC_001425"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="ACG8XC_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP9191131.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="ACG8XC_001426"
     CDS             11221..11514
                     /locus_tag="ACG8XC_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFP9191132.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="ACG8XC_001427"
     CDS             11511..11822
                     /locus_tag="ACG8XC_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFP9191133.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="ACG8XC_001428"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="ACG8XC_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP9191134.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="ACG8XC_001429"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="ACG8XC_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP9191135.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..15127)
                     /locus_tag="ACG8XC_001430"
                     /pseudo
     CDS             complement(14623..15127)
                     /locus_tag="ACG8XC_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15292..17655
                     /locus_tag="ACG8XC_001431"
     CDS             15292..17655
                     /locus_tag="ACG8XC_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFP9191136.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="ACG8XC_001432"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="ACG8XC_001432"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP9191137.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="ACG8XC_001433"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="ACG8XC_001433"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFP9191138.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKKDEQATAEYFSQQ"
BASE COUNT         6863 a         3513 c         4388 g         5232 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg caactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcattaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgatc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg acagtctttt
    17761 ttttcgtttt taggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaaa cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgcct atttgtattg gaaaagccac
    18181 aaaagttatc gagtatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaagaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aagatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtgaaac gattcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggcaaagt tgtcagtatc tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtact
    19021 gggatttttt gacggcgtgc atattggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catcgattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgaggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacagttc atttgcctaa
    19621 agaaggtgtt tatgtatcgg aaataaaaat tggggaaact tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagaat tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcagta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaaaa aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aatttcaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc acagcc
//
LOCUS       DAZKCY010000044        19760 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000044 DAZKCY010000000
VERSION     DAZKCY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.044"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..1135
                     /locus_tag="ACG8XC_001434"
     CDS             95..1135
                     /locus_tag="ACG8XC_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP9191139.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SSPSTITKDWLE"
     gene            1975..2307
                     /gene="traE"
                     /locus_tag="ACG8XC_001435"
     CDS             1975..2307
                     /gene="traE"
                     /locus_tag="ACG8XC_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFP9191140.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            2926..3267
                     /locus_tag="ACG8XC_001436"
     CDS             2926..3267
                     /locus_tag="ACG8XC_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFP9191141.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            3286..3576
                     /locus_tag="ACG8XC_001437"
     CDS             3286..3576
                     /locus_tag="ACG8XC_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191142.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIIDEQKSCVLSIDSTLV"
     gene            3768..4001
                     /locus_tag="ACG8XC_001438"
     CDS             3768..4001
                     /locus_tag="ACG8XC_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191143.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWREHNGIKDLKPARPVIRRKKLKK"
     gene            4068..4321
                     /locus_tag="ACG8XC_001439"
                     /pseudo
     CDS             4068..4321
                     /locus_tag="ACG8XC_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4407..5078
                     /locus_tag="ACG8XC_001440"
     CDS             4407..5078
                     /locus_tag="ACG8XC_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFP9191144.1"
                     /translation="MKKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPID
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLTLPIFK
                     GANQYTLSLGAATYFYEDAEMRKGNYVLAGHNMEIPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            5131..7110
                     /locus_tag="ACG8XC_001441"
     CDS             5131..7110
                     /locus_tag="ACG8XC_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9191145.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDVDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQAL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNNS
                     KGNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELT
                     KFGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVE
                     VPSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVID
                     KTVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDT
                     AGQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASL
                     HGQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLK
                     VDSGLFGTGIKATPLESAEFAAKNAEGKYYGGLVDTDKNGVKEAVWVDDVANAAILKS
                     DKEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKAS
                     VPMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            7125..7877
                     /locus_tag="ACG8XC_001442"
     CDS             7125..7877
                     /locus_tag="ACG8XC_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9191146.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWTWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            7893..8654
                     /locus_tag="ACG8XC_001443"
     CDS             7893..8654
                     /locus_tag="ACG8XC_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP9191147.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLFSDQLKLVSNGKTKTIDQQT
                     GVFEFSIEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            8667..8927
                     /locus_tag="ACG8XC_001444"
     CDS             8667..8927
                     /locus_tag="ACG8XC_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191148.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            8924..11014
                     /locus_tag="ACG8XC_001445"
     CDS             8924..11014
                     /locus_tag="ACG8XC_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFP9191149.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKTTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            11041..11505
                     /locus_tag="ACG8XC_001446"
     CDS             11041..11505
                     /locus_tag="ACG8XC_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191150.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            11502..11744
                     /locus_tag="ACG8XC_001447"
     CDS             11502..11744
                     /locus_tag="ACG8XC_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFP9191151.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            11762..12064
                     /locus_tag="ACG8XC_001448"
     CDS             11762..12064
                     /locus_tag="ACG8XC_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191152.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            12134..14197
                     /locus_tag="ACG8XC_001449"
     CDS             12134..14197
                     /locus_tag="ACG8XC_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191153.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHEGRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYSTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEKENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHEIAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIRERKEEFYQGLRTDKLKISKELNEQLNKLYQIT
                     SEALMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEE
                     AQEQARKEYEQQLEELLNRTNQATTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            14197..16806
                     /locus_tag="ACG8XC_001450"
     CDS             14197..16806
                     /locus_tag="ACG8XC_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFP9191154.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAILMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPENDYRQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHQGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     TNSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLHQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            16803..19199
                     /locus_tag="ACG8XC_001451"
     CDS             16803..19199
                     /locus_tag="ACG8XC_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFP9191155.1"
                     /translation="MIIGGIFSGIANWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLETDPNKVESLPTDKRNALTAKNFKTANINSVII
                     PKEAAKMADEDKSGLSDIQKDRLRALQYRLELDDNGKEIPVEISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSNLNDIRKNGF
                     GNRKDVIPDNGEEQPDDKATIVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQY
                     RYKSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSMKIGLPKTQNLP
                     MKDSRLNQFKENDQSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNVSIQN
                     VVTREAAPEPIQKVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP
                     "
     gene            19251..19583
                     /locus_tag="ACG8XC_001452"
     CDS             19251..19583
                     /locus_tag="ACG8XC_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191156.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
BASE COUNT         7329 a         3001 c         3946 g         5484 t
ORIGIN      
        1 ttaatgcgtt tgcatttttt atttatcttt gctattttcg aaaagagtag gtaattttac
       61 ataaataaaa caacgtgaaa gtagaggatc tacaatgact gattttaatt tttttaaggc
      121 ggatagagtt tacaacgaat tattttatca atttccaaaa gtctttattg tttctgacga
      181 atacaaaaaa atgaaagatt caactaagat tgcctatatg cttttaaaag caagattaga
      241 gatcgcaatc agcaaacggc aaatcgatga agaaggtaat gtttatttta cttatacgac
      301 aaatgaacta tgtagcgtat taaactgcca aaaacaaaaa gcgatagcaa tcaaaaaaga
      361 gttggaatcc tttggtttat tattacaaaa gcagatggga tttaacaaac agttagggaa
      421 aaataatcct aatagactat atctagcaga attaaaagtc tcagaaaatg atatctactt
      481 actcgaaaaa tttgatagag agaataggga aaatgttgat aaatcagagg gtatgaaaat
      541 catacccacc ctcgacgaaa aatcagacgc tgaatccctt ggggctcaag agggtatgaa
      601 aatcataccg tgccaaaacg ttgataaatc agagggtatg aaaatcatac cagaacttaa
      661 taataatata ttagacacta atagacacaa tatagacact gaaaaagacc gcctacaaga
      721 tcaattgttg ttagacaatt ttgagacaat tatgacaaac gacagcattg ctacgtttgt
      781 ccctgaacga gtattaaatt tgataaaaac attttcttca agttacagtg aagctcaaaa
      841 aaccgtccag actattcata atgcaaagaa aaaagctgaa atagaaagtg gtatttcgat
      901 agtttttgaa gaactcgata gttattatgt caatgcagaa caagaattat acacgacact
      961 gttaaaagcc tatcaaaaat taaaaaccga aaaagtcgaa aatatccaga acctgatttt
     1021 tgtctatgta aaaaattggt ttatcgaaaa accaatagct gctaaagtat caagtgaaaa
     1081 acgtttgaat tctgaaagct ccccaagcac tattacgaaa gactggttag agtgaaactt
     1141 tactgacaaa gtgattgttg cttcatggag taacattaaa gttaaaaatt ttttattatt
     1201 ctaattatat tacgaatatt acaaaataat agctatttta atagaattta taagttaaaa
     1261 tattatgata aaaatcacaa acgtagcctt ttacgtcata agtgttaaaa gaaataatta
     1321 atttcaaata taaaaaaggg aacgtatgtt tgcatactcc ttttttttga gttataataa
     1381 tacgcgaaga gagataattt cattatttaa acaaactaaa aatcttgata ttacttcttt
     1441 tcaggcaata cgaatattat tcgcttacat ttttttgtat aaatttagtt gttttaaatt
     1501 taattgtctg ctatgcctgt gatgaattga ttttcgttct gcacttttgg acgataaatt
     1561 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
     1621 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
     1681 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
     1741 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cataatgtta
     1801 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
     1861 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
     1921 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
     1981 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
     2041 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
     2101 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
     2161 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
     2221 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     2281 aaagggatgg ggcgtttaat cagctgaatt gatcattctc ataacttgta ttttatcgtt
     2341 tttcgcttgc ttgaaaatat tgtcgagact tctaaaagaa cttagaatca ttagaaaaat
     2401 aaggagtaga cttgatctat caacaatttc cttcggggag tgacggggta gatttttacg
     2461 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     2521 gaatttgtgt aaataatata cacagagtaa acaagaggga atttagaggt tagacaagtt
     2581 agaatagcgc ctagattaat catgagcagc tactaaggga tctaggcttt gacgcaaact
     2641 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     2701 gctgcagtac atgtagatac ttgcgaggac gttggtcatt gggtcaatcg agcatttacc
     2761 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgatattt tttatgtatt
     2821 tttaagtaaa cgaaaaacga taaaatatga acgagtcctg aataaagtga aaaaattgat
     2881 ataaaacata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     2941 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     3001 ggttttgtga cgtttagaag aggcggaaat tgtaaaggat tagatgtttt agcattaaac
     3061 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     3121 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     3181 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagcgt tgaaatcatt
     3241 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattatgaa atttttcgaa
     3301 gtaaaatcac catactatta tgcgttaatc gcagcaaaga atgaagaaga atgtgcagaa
     3361 ttgtatgaac aagtagtaac agatatcgaa aatcgtgaag aatttattcg atgtttgcat
     3421 gagttaaatc gaaatgaagc aattcaagag gatgcgaaaa aaattagtga agaaacgtta
     3481 caaccaatcg gtatggagga agctacaaaa gacattttta caattattga tgaacaaaaa
     3541 tcttgtgtat taagcattga ttctacattg gtttaataga tccaagattc tataattttt
     3601 ttcaaagtaa aaagaaatta ggtaatgatt ctcagaccaa ttacttagga aaataaccat
     3661 acaaattaga ttttacagat aaaggattag aaggaatcac ggcgattaat aaaaaggtag
     3721 tattacctta ggcattgagt aaggaattta tttaggagga aaattagatg gcgcgaccag
     3781 ttttagatgc gatggatatg gatactttga caaaaggaag gtatgtacag ctgttagctc
     3841 gtggttattc acctaaagat gtattcaaag ccctatctaa aggaacagat atggaaaaag
     3901 aaagattacg aaaagaattc gactattgga gagagcataa cggaataaaa gatttaaaac
     3961 cagcacgtcc agttattaga cgaaaaaaat tgaagaagta agaccaaagc gtatggcgta
     4021 cataggcttt tttattttga taaaaataat gaaaggaagg aaaactcatg aattgtaaag
     4081 aaatcgaaaa tagaaaaaaa gtgtcgaaag aaatggaaga aaagttacta aaaacaatga
     4141 aacaaaaaca atgaaacaaa aacaccttaa aagattatct gtcgcgcaat atatcaatga
     4201 tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa actttgaaca
     4261 gttgagacga acttatgtga aaaaaagttc taattgtcaa ctattgttag aagtctcatg
     4321 acattcatta atagaattca tttttaaaac tttttctatg gaaatgagaa aaagtttttt
     4381 tgttttgtct gaaaggaggc agatggatga aaaaatggtt atttggtttt ttaggagtcg
     4441 ctttaatcgt cttttgcagt gtgtttggct atgttagcta ccaaaaacat gaaggagagg
     4501 tgtttaagca aaatatagaa aaaaagatgc ccatagatca aatcaatgaa catgcaaaga
     4561 gttataagga ggatgcaacg aacgttaata atgatatgtc tttgggacaa atgttgtcca
     4621 tacaaaaaga agcaatcgaa atgggcgtca ataaacaagc ctttgcacaa attcagattc
     4681 ctgcgcttgg tttaacattg ccgattttta aaggtgcaaa tcaatacaca ttgagtcttg
     4741 gtgcggctac ttatttttat gaggatgccg aaatgcgcaa aggaaactat gttctagcag
     4801 gtcataacat ggaaattcca ggtgttttat tttctgatat tcaaaagttg tcactagggg
     4861 aggtgatgga cttagttagt aacgatggcg tctatcgtta caaggtaacg cgtaaattta
     4921 tcgtacctga gtactttaaa ttaatcgatg gcgttcctga agagaactct ttcttgtctt
     4981 taccaaagaa aggagagaaa cccttattaa cgttgtttac ttgtgtttat acgtcacaag
     5041 gaaaagaaag atacgtggta cagggagagc tacaataaca aggttttaat caacgtgagt
     5101 cgttgaaata tgaaacgagg agagtaaaaa atggcaataa atcggtatgt aaaaaaagct
     5161 ggaatgcttg ttggaatgtt aggaatcatt gcaggctgct ttcagggata tcgagcatat
     5221 gcagaagatg tcacgcaaaa aacgccacca gagaaggtca atatcacggt tcataaatta
     5281 atgtatgatc aaggaacgca attgaatgtt gatgttgacg ggatcaaaaa cgatggttat
     5341 acgcacgatc aatatcctac gggtgtaacg aaatacaaca aggcagatta tggtgatgtt
     5401 gagtttacgt taggaaatat tacggatcaa gcattaccga gggaagattc tgatctaacc
     5461 aacgcgaaag tggacgaaat cgttaaagat gtagaagaca agggatctaa cagtgagtac
     5521 gtcaagaatg caaccaacaa aattacttca gctgtagatg aaaatggtga gattactttt
     5581 gctgatcaac cagcctatgt caacaattcc aaagggaatg tatatgtggt ttatgaaagt
     5641 aaatccgctg ctggattagt cacacaaaaa gcaaaaccaa tggttgtgat tgcgccaatg
     5701 actgataata caggttcagg ttttttaaaa gacattcact tatatcctaa aaatattgtt
     5761 tctaagttga gttttgaatt aacgaaattt ggtgacgatg gcacagctca aagcaaacaa
     5821 acacctttaa aaggggcaaa atttgagtta tacaagggcg aacctggaaa aggaacaaaa
     5881 ttaggagatc ttgtatcgga tgatcaagga aagctgactg ctactgattt gacgttagga
     5941 aaatactatt ttgtggaagt tccttcagaa gttgtagtgg gatcagataa agaaccaaca
     6001 gccgatcaat atttattagg cgcagacgct cgcaacgatg cgcacaataa attaaccttt
     6061 gaaattacga atgatggcgt gacaagtgac ttaaaagctt catacgtaaa ctataaagca
     6121 ccagtgatcg ataaaacagt aacaaatgga acaggacaag aacattcttt ccaaattggt
     6181 gacgcagtaa attatcaagg aacgattcat atcccaacag acattgcagg cggagcagac
     6241 ggtatcacag taaacggagt gaaaagcgaa acttctcctt acagtgtatt taaatggggg
     6301 gatacagcag gacaaggact ttcttatgtc gctgctaaag caaatatcaa agtaacaaat
     6361 aaagatggat ctgtggtatt aaaagaaaat acagattata aaatccaaaa ttcagaaaat
     6421 ggctttgtga tcgattttat cgtgaataat ggccaagtaa gtgatactgt agcaagttta
     6481 cacggacaag atcttcaaat gacttataac atgtatgtaa atgacagcgc agctgttgcc
     6541 aatccattaa caaatagcgt ggatttcgtt tataacaaca atccatttaa tcaggaagaa
     6601 caccacgaaa aaacgaaagc tgacgttgtg acttatggtg caaaattctt aaaagtcgat
     6661 agcggactat ttggaacagg aatcaaagca acaccgcttg aaagcgcaga atttgctgct
     6721 aaaaacgcag aaggaaaata ttatggtgga ttagttgata cggataaaaa cggtgtcaaa
     6781 gaagcagtat gggtagacga tgtagcaaat gcagcgatcc taaaatcaga taaagaaggt
     6841 cactttgaaa tcactggttt aactgaaggt gaatattcac tggaagaaac aaaagcgcct
     6901 gaaaactatc aaaaattaac gaaagagatc tctttcaaag tagacaaaga ttcatacaaa
     6961 gaagaaaacc gaattacgat caaaaacaat caaaaagctt ccgtaccaat gaccggtagc
     7021 aacggttttc aaacgtatgt attaatctct tgcttgttgc taggagcagg ggcactttct
     7081 gcagttgttt actttaagaa gaaagcctag aaggatcaaa accaatgatt atcaggaagc
     7141 ctaagaaaaa gcgaatcatg ggtaaatgga tcattgcttt ctggttactt tcagctgtag
     7201 gtgtcttact tttgatgcct gctgaagcga gcgtggcgaa ataccaacaa aatcagcaga
     7261 ttgccgcaat cgatcgaaca ggaacggcag cagaaacgga ttcttctttg gatgttgcta
     7321 agattgagtt aggcgatcct gttggtattc ttacaattcc aagtatttct ttaaaactac
     7381 caatttatga cggaacgtcc gacaaaattt tagaaaatgg tgtaggtatt acggaaggaa
     7441 ctggtgatat tacaggagga aacggtaaaa atcctttaat cgcaggtcat agtggacttt
     7501 ataaagataa tctttttgat gatttacctt cagtaaaaaa gggagaaaag ttttatatca
     7561 aagttgatgg tgagcagcac gcttatcaaa tcgatcggat tgaggaagtg caaaaagacg
     7621 aactacaaag aaactttgtc acttacttag aaccaaatcc caatgaagat cgagtaacgc
     7681 taatgacatg tacgcctaag gggatcaaca cacaccgttt tctagtgtat gggaagcgag
     7741 taacctttac aaaatcagaa ttaaaagatg aagaaaataa gaaacaaaaa ttatcttgga
     7801 catggctttt aggaagtact gtttttcttt cagtcatgat cattggttcg ctatttgttt
     7861 ataaaaagaa aaaataaagg agggtaacca acatgagaaa tagaaaatgg ctatttgttt
     7921 gtgcagccgc tcttttttct ttcactgcct acacacagtt gacgtttgca gacagacaag
     7981 aaagcacgga tagcacagaa caaacaatcc aaacgtcaga tagcagcacg attcaaagca
     8041 gcgatgaaac cgtcgattcg tcaacagttg attcttcaga agaaccgaaa attgctgaag
     8101 acgatcaaat agtcaaacag accattcacg ttcaaaaaat tatttcttcc gaacaattag
     8161 acaaccaggg gcaaaaactt gaaaaacaag aaggagtaaa tggcgcgaag ttttctgttt
     8221 atgatgtatc ggacatttta caaaagatgg atgttaagga tctcactact gaccaaattg
     8281 aatcccaatt aaaagatcga gtgaaaaaac tattcagtga tcaattgaag ctagtttcta
     8341 atggaaaaac aaagacaatt gatcaacaaa caggtgtctt tgaattctcg atagaagtac
     8401 aggcaaatca gaaacaagct tattatattg tcaatgaatc aagtcctgaa aacatttcta
     8461 atagcgagga tattttgcta ttaacacctg tttctgataa aaacggtgaa ttcttaaagg
     8521 atgtgtggat ctatcctaaa tcagaagctt cgcaacctaa agaagaagta aaaaaaatcg
     8581 tttctactgg cgtaaagaaa aacttctttg agaattgttg ggattttgtt actcacttgt
     8641 tttctagaga ttaaggaaga aataaaatga aactgatcaa attattatta ttcaattttt
     8701 cttgtttaac cgcagtcttt ttgtactgcg gtttattatt ttctaaacta tctattttgc
     8761 caagctacca actataccta atgaggattt taaacatacc agctgcttcc atgccgctag
     8821 atattatgga aaagctcatt aggaatccat tatttactac gatcaatttg atcgttattg
     8881 tcggaatttc actagtcatt tataaaaaag aggggatgaa gggatgaatt ttaaacactc
     8941 gtggacaaga cggttaaaat atgtttttcc cataatgatg atgttaggta cattgtttgg
     9001 gttaaaaaca gccaatgtct ttgctgaaaa agttattgtc gatccgtcaa atccaatcca
     9061 gaacgtaaag aataatttta ttattccaaa agatgttcct aatgcaaaaa caaatattac
     9121 tgtaaacgga gcgtcaaaag tttacaaata tcatgttgat gccaacaaag gtggttttac
     9181 agacgactac gtaggattag tggagggaaa caaaaatata cgctatcctt ctttccaaaa
     9241 agaatacggt gaaacaaacc ttgttttaga aggcgtaaca accaacacca aagtcaatat
     9301 tgactatgga aaaatcggta cgtataacgg gaaaaaagtc aatatcaagc tagttctatc
     9361 taatattcac ttatactcgg atacgttgcc gtggaatcta ctagataaca actatacaaa
     9421 aactcatttt cgagacgatg gctataaaaa tacgaatggt gcaatgagta aatctaaaaa
     9481 aagaacagta ctttggatct cagacaatct tttctcagga attgtttacc attcaacaca
     9541 aatgaatgtg cagctagtag ccacatatga ggacggtagc cccgttcaat tttcaggaga
     9601 tacatttatc agttttaact ctttgaaccc tgcaggagga aaaaccacgg atcttaaagg
     9661 agaatatgcc cactatgaca aaatgaatac gacagattgg tatgtcagaa aagatacggt
     9721 tctttcagaa tttaaatctt tctacaacaa tctgaacgtg gtaggtggcc acccaggagg
     9781 aagctctaag ctgacacaag cggataatga tttcaataat ttgcatgata aattaggaga
     9841 tccgaagttt ggtcaaggta ctgtgtcctt taaaatatct gaagcaaatc cgacgtttgt
     9901 gattggttct tctaatgttc aaacctggtt tactttatct tctgcgacaa tctttagtgt
     9961 tgttcctgat caacctgaaa aaacaggggt tgataaaaat ggaaataacg tgaacgataa
    10021 aatgttgcaa gtaggagaca cgattcaata tcgaatcaaa cagaaggtga atcgattagg
    10081 agtggattta ctagcggttt acgatcgatt tgagttaatc gataatttac caaaagaagt
    10141 aaattacgtc gatgcacacg ttgaaagtgg tacaaataaa aaattcgatg tatctggtga
    10201 agtgacctat gacaaaacaa aacaccaggt aaaatatgca gcgaaagctg atacattaaa
    10261 aaatcgaatg aagtataacg gcgaaactta cgaattagtg atcaatgtaa aagtcaatga
    10321 attggccaat caaaatagtg tagccaaaaa tcagggaacg tcgatcatta ataaagtaga
    10381 aaaggataca aatatcatta ccgtttattt ccctaaaatt ccagtgaaag aagtacaaca
    10441 aaatggaaaa gatgtaaacg gacgaaatga cggtaagaag ggtacgccga cagctccttt
    10501 aaacgcagga tctgaagtac aatatctagt cacacaaaaa tggcacacaa aaggcgtaga
    10561 cgctgcgtca gatcactaca aacaatttag tattcaagat ccaattgaag ctcgtttgac
    10621 atataaagaa ggttctgcgc aagtaataga taagagtaaa ggaaaagata ttacttctga
    10681 aggcacacta acctatgata gcaattcaag aacgctgaag tgggaagctt ccgctgactt
    10741 cttaagtaac aatcttttag atggacgaga aattcagctg atatttacag ctaaaactcc
    10801 attacaatca gaaaaaaata ttgataacca agccgtagta gcagtagata atgtttctaa
    10861 taaaacaaat gttgtgacaa ttggggttga tcctaacttg ccacaggtca ttgttcctaa
    10921 aacaggttct acacatttag taacgatttc agcggtcagc ttactattac ttgtgttagc
    10981 aacttttagt tatgcggttc tgagatacat ttaaaataaa aaaacgaaag aatgaataaa
    11041 atgaaaaagt taataatagt agttacttcc ttgattgcca tgattgttat tttagtcgga
    11101 tgtagtaatg aaaaaaataa acaaactaat caagataatg gcgtattgaa atcaggaact
    11161 atgtggaaag aagaagtagg tggtcttgtt tataatttga aaattataga cgagactacc
    11221 tgggaatact ctgagagtgt atggcatcca gatcctgtac aaataacagt caagaggcaa
    11281 aaagactata aaggtttaga aagatacaaa atagtagatt cagctggtgt tagggaattt
    11341 ataaacaaat ctgattcttt atttattgtt gttccctatg agaagaatgg cgtaaaaaaa
    11401 ataatttttc ttggatctag caaggatgaa aagcgaacga aagagaaatt gattcacgat
    11461 ggatcacaat ccaataaata taagttacaa aagactagcg agtgattgct agtcttttta
    11521 tttgtcctga aggaggaatc acagtggcaa aagctaaaac gccatttgaa aaagccgcgt
    11581 ctaaaatctt agaacgccgc ggtgtatctg aaacacagtg gaaacaagaa gtccttgaaa
    11641 aagcacaatt tgacctatta cggggaaaag acaaggactt agaaggatat atctatcaac
    11701 gtgaacgtga aaagcttgtt ttcgacgaaa ttattaaaca ctaaaaggag cgaacaattt
    11761 tatgtttgaa tcaattacaa tgaatttttt aacacaaccg aaagtagtag ctgcggttga
    11821 tatttttaga gcattacaaa ctgccgcaac tagtttaaaa aacaatctga tgatttttgc
    11881 tgctgctgcg gccgttgctt ccattgttat tgggggtctt ttcttcctat ttggacgaag
    11941 aggagctgaa aatgggaaga tgcacgtagg ctatgtattg atgggggtag gagttatttt
    12001 agcagcagct tctattgtgg tttcgttatt tggattgttt ggacaaacac ctcaaggatt
    12061 ataagaaagt aaaaaagtag gagtaagaag tagtcgatac tttttactcc ttttttatga
    12121 aaggagaata ctcatgtact ttacgattgc aggcaatacc aaaacagata aagagttaat
    12181 aaaaaaagca acgaaggaag taggaaagcg tacttctttt ttaccgaaaa caattaaata
    12241 ttctgtcatt gaaacggaag aacagctaca tgtgttaggg aaagtactgg aacacgaagg
    12301 tcgatcttct gttaagttga ctctcacgtt acatgacaca gaaggaaaaa tagagaaagc
    12361 tggtacagaa tacgtttttg gcccactgga ttatcaaatt tcagaaggtt tctattctac
    12421 attgatggat ttaatccgcc ttgatgtgtt taaagaaacg gatgaaacta tgagtgtgga
    12481 agagtcttat cgaaacgaaa aacttttaca agttttagaa gaagagttag agagcgacca
    12541 aagaaaagtg aatcaaggga gtgaagtcga agtaaatgaa gaagtaccat gggaacgacc
    12601 aatatttcca aagaaggagt caaaagaaaa agagacagtc tatgttgacg aacaagctga
    12661 tattccgact cagaaatcga tatttgagaa agagaacgat gtgatgggtc agttggaggt
    12721 agatcccttt gaagaaacaa tggaggatga agagacggcc gaagtagaag ttgatccagt
    12781 tgaagctaat gaagagtcaa catggactga aggggattct caggaatata ttgaaccact
    12841 tatcagtgaa gaagtagaaa aaaacgaaat agagatacaa aaggcaactg tatttgatta
    12901 tattttggat caatatccgc cagagcatga atggatgaaa gaaaaaaaca aattattttt
    12961 aaatgagctt tattcgaaat atcaattacc ccaaacctat caagcatttt tagagagtaa
    13021 agaagaatta atctcacagg gtaaaggcac acttttagaa cagttagagt ctgtccaatc
    13081 aacaaattgg catgagattg cacaagaaga tttattagcc acctttgaag aacgtcaaca
    13141 agaaacagat caagaaatta atgagtatgc gacggaacag tcgtctaatt gggagaaagc
    13201 aaaagaggaa ctaaataaag aagaacagcg agtgattgaa gaagaagtac aaaaaattaa
    13261 agagcgatac gaaaagaaac gcgacaatgc gtattccttg tgtgtagaaa agatcaataa
    13321 ttttactcag acgaatcaag aagcgttatc gaaagaaaaa gaaaacttgt taaaagaacg
    13381 tataagagaa cgaaaagaag aattttatca aggtttgcgt acagataagc taaagattag
    13441 caaagaatta aatgagcaac tgaataagct ctatcaaata acaagtgaag ctcttatgga
    13501 aaaacacgag cagatccagc aagaattaca agtacaaatt ccaaaatgga aaaaagagta
    13561 tgaaattcaa caacaagaat tgaaagaaca agaagaaaaa caacggcaaa aagaaaaaga
    13621 gcgcgaacaa ttagaactaa aaaaacgtga acaggatctt gcggaacaac aaatggcaat
    13681 agaaaaacaa cggttaaacc aagaaaagga aaaagaagca gctgctcgta aggaacgtca
    13741 agaacaaatg gatacattac gacaagctca gttaaattat ctagcacaac ctcaaccaac
    13801 gccttcagta gctgctgttc aaaccccaat gaacaaccaa acaaatctaa ctccttcacc
    13861 acagcgttcc tttaacggat ggatggtggg atgtattgca agtttaagct tgcttctagg
    13921 tggagggaca gtatttgctt ttaatcatat caatcaatct tcacaggctg attctgtagc
    13981 cacaacggcg tcaatcaagg aagaagccca agaacaagca agaaaagagt atgaacaaca
    14041 attggaagaa ttattgaatc gtaccaatca ggcaacaaca gagtcaagta tggaaaatag
    14101 caaagagagt gatcctagta aggctaaaga aggatcaagc gattcaactg tacaatcaaa
    14161 cacttccgaa agttcagaat caaatgaggt gaaataatgc gtgttagaga aacaacgaga
    14221 cgaaaagaca aacgattcaa tttagaaaag aataggtatc tttctcattt ctatcgctat
    14281 actccttcag gagaagagct accagaaaaa catgacaaac gtatttctca aaagattttt
    14341 tatagtggct tagcttatct tcctttaaac tatgttgtta caagaatacc agaagcattt
    14401 catcgatttg agattattcc aggaagttta caaggagtga atatttttca gttacaagaa
    14461 atgccgcagt tattccattt tgattcaact ggtttttgga atcctttgac cccaacaaca
    14521 agtttaggca cacgtttagt tgtttctacg atttgtgttg ttccagctat acttatggga
    14581 ttgaaaatgg acatgtggtt tcgtcctcta gcagatgggc aaaaaggaga taaccgatta
    14641 aaaacgttaa aggaaatcca acaaatttat ccgcaaatac cagaatcaaa gggaggtttt
    14701 cctggttatg gcggaatacc aattactcat tataaaaagt actggtatat tcagacggat
    14761 acagtaaata cttgtattgt tggtacttcc cgttcaggga aaggacagat agaagtttta
    14821 gcaaccattg acaatttgtc tcgtgcagaa agacaaagtt caatggttgt taatgatcca
    14881 aaaacagaat tatatgttgc tagtaaagat gaactagaag ctagagggta tgatgtgtat
    14941 gcatttaata tcaccgatcc actgcaatca atgagtgata atccattggc actcatcgtg
    15001 aaatactgga agcgaggaga catagatact gcaacgcagt taaccaatac ttttacgaat
    15061 acgatttatt atgatgccaa tgcttcagac aacaaatact ttaacgaaaa tgcgcagaaa
    15121 gctgtaaatg cgcttatttt tgcgttgtta gaacaagccg atcaaacagg ggattactcc
    15181 aaattaacac ccaataatct tgttgagctt ctttctgaaa ttggcggttt taattatcaa
    15241 gatcctgaga atgattacag acaactcaat gccttagatg aattcatgaa tcagttacca
    15301 cctggaaaca tagcaaaaaa acaatatggg gcaacaaaaa ttggttctga taaagcaaaa
    15361 gggaatattt tatcgacggc aatcacagga ttgaatccat ttaccctaac gaaaattgcg
    15421 aaaatgactt ctcaatcaac cattgattat aagtccattg gttttccgaa gtatttggat
    15481 ctcaaactac acgagagtct gttaaatcag cgaattgttg tagagtttta tcatcaaggg
    15541 aaaaaatttc atgaggaacg tgtcaaagta ggttatagag gtttttgtga aatcaacttt
    15601 gattgtcatt tagagcaggg agatcatgtt tgcttaaaat acgatcatca aggaaaagaa
    15661 tatgttgctt ggtatgagtt ttctcaaaga gtattgaaag acgaagaggg aaatatcgtt
    15721 tatcgaaaaa aacgaggaga aacccattta ccagaattag aaaaagaagc tgttctaaca
    15781 ttagatgagg ataaaagtaa cttattagta gagtctattc ggatgcacta taccgataaa
    15841 ccaatggcta tttttatgct cacacctgat tatgatccat ctaaccatat cattgttagt
    15901 atttttctta gtcagttgta taaagaatta tctacgcaat gtgttaagac taaaggagat
    15961 aaatgtcacc gtaggattca ttttatttta gatgaatttg ggaatatgcc accaatggat
    16021 aatatggaag gaatcatgac agtcacagct ggtagaaaca tgttgtggga tctgtttatt
    16081 caatcctatc agcaacttta tgcgaagtat ggggaacaaa acgcagcgat tatcaaagaa
    16141 aattgtcaga cacatattta tattatgtcg acagacgata ataccatcga agaattttcc
    16201 aaaaaagtgg ggaataagac ggttgaacaa gagaacagta ccaccaattc attaggtatg
    16261 aatactcata tcaatcgcaa tgttgattca gatcgtatct taactcctga aagaatttct
    16321 acttttttag aaggtgaaac gattgtactc gctcctttaa aacgacaaga tttgaaaggt
    16381 cataaaatac ggccgtatcc tatttttaat catggggaaa cgaatcttcc ttatgcttat
    16441 caatatattg aagatttcaa tccatcgtgg gatatcaatg atatagacat acattgtaca
    16501 catgcggact tggatcttaa ggctttacaa attgattacg attattttct acctgatatc
    16561 tatgcacgtc gactttatca caatatgcac gatcaaatag aagaggaagc taaagaaaac
    16621 atttttatta aaatcgttca atctttagac aactcaacgg cacaaaagtt acttattcaa
    16681 ttttatgaag agaagcaaat agatcgtttg caagaatacc ttgaaaaatt gcatcaaaat
    16741 gaagaaatta cagaggttat aaggaaacga cttttgctag aagctgaaaa ggcggtgaaa
    16801 gaatgatcat tggaggaata ttttccggca ttgccaactg gtggtcaggc ttatttgggg
    16861 atggggatgc agatcccaaa accgtaacga aagtcttaca agattatggt aattatctgc
    16921 agtatcaaga tgtattcagt tggatttgta atactttttt atggggtttg attaaagggt
    16981 tatataatct caatacttta ctagaaaaaa caatttatca aagctttgat ttaaaagact
    17041 tattgaacgc tgctggtgtt aatgaactgt ttcaaagttt gattagtaag gtattggctc
    17101 ttttttgtgt cgtcacatta atttatttag gcttaaaatt ttttatgagt aaacatccac
    17161 caaaaatcaa aaatatcttg gttaatctat ttatggccat gattttgatt tttggtggta
    17221 gttctttaat cgatgaaggg ctaaatatta gtaaaaactt ttacggagac gtgactgcag
    17281 caaataaagc agataaaact ggatcacctg cttttcaaat tattcaaaat aatgtgatcg
    17341 atgtgagtac gctactagaa actgatccga ataaagtaga atccctacca acagataaaa
    17401 gaaatgctct cacggcgaaa aactttaaga cagcaaatat taattcagtg attattccta
    17461 aggaagccgc taagatggcg gatgaagata agagcggtct atcagatata caaaaggatc
    17521 ggctaagagc gttacaatac cgactggaac tggatgataa tggaaaagaa atccctgtag
    17581 agattagtga tgaggggtta gcaaaatatg tgtatacttc aggctatcgc cgatacatta
    17641 gtagtccagg gattatttta gctggtgaat cttctttgtc ggttgcctat ttatttatct
    17701 tgtttacgat tgtaacttgc attattgagc tggtatttaa aaaattctat ttagtaattg
    17761 ccgcgtcaac ggactttgag acaggacaac ggatgaaaag tgctatagaa gatatttctc
    17821 aatccttctt gttattggcg tttacgattt tagaattacg aatctataca ttaatgctgt
    17881 caggtatcgg tgatttgcac gcagcaggca agatcaacgg atttttgtat gttgtcgctt
    17941 taattgtact aaccattgcg ctattcaaag gctctcaatc tgttacgaag attttcggtg
    18001 ttgatacaag tctgaaaaac ggcagcaatt ctcttcttag catgtttgcc ttaggaaata
    18061 ttgcgaaaaa tgtgggtagt ggggctaaaa accttgcaag tgctggtaag agtggtttaa
    18121 gcaatttaaa tgatattcgt aaaaatggat ttggcaatcg aaaagatgtt attcctgata
    18181 acggggaaga acaaccagac gataaagcta caattgtacc taatagtaag aaaacagttt
    18241 ccgaaatgtt cgggaaagcg aaaaatggtg tttctaaagc cgcagaaggt cttggttacg
    18301 tcaatgaacg tgggttaaaa ggaacagctg aagatcttac agaaaaagcc aaagacacct
    18361 tagaagagaa attagacggc acgagagcta aagaattggc tgaagctgtg aagaatccat
    18421 ccgaagcact agaaaaacaa aaagaaaaag cttcagaagt caaagaagga ctaaaagatg
    18481 cgtatggtga aggacaagtc aatgcagcag tgaaggcaaa tacaacaagt gaaaaagaaa
    18541 atcaagaagg tgcagcgatt ccttcagcca aagatcacca agatgagtta cattctaacg
    18601 aaaattcgat cggagaaaaa gattcagaag aaggacaaca tggacagtta ccaaaaaccg
    18661 atccagaaca atatcgttat aaatctaaca ttaattctga aaatgaaaag cgcatttcag
    18721 atttaccaaa acgaaatcaa gaagtacaaa cggatgagga ttccatcgat ttgccaaatg
    18781 ataatggaat cgaagttgga actgaaaaat caatgaaaat tggtttgcct aagacacaaa
    18841 atttaccaat gaaagatagt cgtttgaatc aattcaagga gaatgatcaa agctttgttc
    18901 accaatcaac tgcaggtact gttcaaatga aaccaagtcg tttgcctgaa aatggaaatc
    18961 tgacaactgt ttcttacaat ttatctgaaa gtggtgagac agggaatgtg tccattcaaa
    19021 atgttgtgac aagagaagct gcaccagaac ctattcaaaa agttcacaca ggttacaaac
    19081 cagcaaatgt ggcatttact gcaccaacaa aaataaatgt aacaagagaa actaaaaaca
    19141 ggaacaatga attgaaagaa caactattga gggatatcaa acaggatatc aaaccataaa
    19201 aggcctacga gttaggcctt ttttctatgt atgaaaagga gaaatcattt atggataaat
    19261 atattaacat gaaaaatcct ccaagattta gtggggaatg gaagtttttc gcctttttct
    19321 ttttagatct attaacgctt ggtggttgct ttttttgggc gaaaatagtc aataatcttg
    19381 tccacttacc actctttttt gcactcttga attatggctt atggattgcg atagctctag
    19441 tgattgtttg gcgaccttat aataacccag gtcaacgaca aatttttgcg attatcgaat
    19501 ctattttctt tgcggataag aaccactatc atgcgttaga tcctaaccga aattatcatg
    19561 gtacgtataa ggaggatgaa taaatgcgag tcaaaggaca aaattcagtt gataagaaat
    19621 ccattttgca gtttggaaaa ccagaagaac gatctatctt ggatttattt ccttataaat
    19681 cctgtacaga agagggagtc ttagttacta aagaagatca ttttcaacgt tattatcgtg
    19741 tagtttctac agacgtggaa
//
LOCUS       DAZKCY010000045        19548 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000045 DAZKCY010000000
VERSION     DAZKCY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.045"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACG8XC_001453"
     CDS             complement(287..649)
                     /locus_tag="ACG8XC_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191157.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACG8XC_001454"
     CDS             complement(723..1103)
                     /locus_tag="ACG8XC_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFP9191158.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACG8XC_001455"
     CDS             1185..2360
                     /locus_tag="ACG8XC_001455"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFP9191159.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACG8XC_001456"
     CDS             complement(2437..3021)
                     /locus_tag="ACG8XC_001456"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9191160.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ACG8XC_001457"
     CDS             3222..4220
                     /locus_tag="ACG8XC_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9191161.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ACG8XC_001458"
     CDS             4332..4841
                     /locus_tag="ACG8XC_001458"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP9191162.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="ACG8XC_001459"
     CDS             complement(4871..5530)
                     /locus_tag="ACG8XC_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFP9191163.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="ACG8XC_001460"
     CDS             complement(5532..6296)
                     /locus_tag="ACG8XC_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFP9191164.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="ACG8XC_001461"
     CDS             complement(6304..6981)
                     /locus_tag="ACG8XC_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFP9191165.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="ACG8XC_001462"
     CDS             complement(7018..8403)
                     /locus_tag="ACG8XC_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFP9191166.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="ACG8XC_001463"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8XC_001463"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACG8XC_001464"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACG8XC_001464"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP9191167.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="ACG8XC_001465"
     CDS             9342..10199
                     /locus_tag="ACG8XC_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9191168.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="ACG8XC_001466"
     CDS             complement(10280..10615)
                     /locus_tag="ACG8XC_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191169.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="ACG8XC_001467"
     CDS             complement(10682..12052)
                     /locus_tag="ACG8XC_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFP9191170.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="ACG8XC_001468"
     CDS             complement(12130..12666)
                     /locus_tag="ACG8XC_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9191171.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="ACG8XC_001469"
     CDS             12975..14456
                     /locus_tag="ACG8XC_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9191172.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGELIKAVHELYMKKGYENKTKRM"
     gene            complement(14559..15266)
                     /locus_tag="ACG8XC_001470"
     CDS             complement(14559..15266)
                     /locus_tag="ACG8XC_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFP9191173.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15345..15683)
                     /locus_tag="ACG8XC_001471"
     CDS             complement(15345..15683)
                     /locus_tag="ACG8XC_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP9191174.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15794..16756)
                     /locus_tag="ACG8XC_001472"
     CDS             complement(15794..16756)
                     /locus_tag="ACG8XC_001472"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFP9191175.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16771..18102)
                     /locus_tag="ACG8XC_001473"
     CDS             complement(16771..18102)
                     /locus_tag="ACG8XC_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFP9191176.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18110..18790)
                     /locus_tag="ACG8XC_001474"
     CDS             complement(18110..18790)
                     /locus_tag="ACG8XC_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9191177.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19009..19488)
                     /locus_tag="ACG8XC_001475"
     CDS             complement(19009..19488)
                     /locus_tag="ACG8XC_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFP9191178.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
BASE COUNT         5828 a         4023 c         3237 g         6460 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcgag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta ttccaaataa atggtattca atcagctaac tctcggtatt
    14521 agctcagatc aaagcgctga tttcacaact tctttatttt aatctcttcg gttgccgccg
    14581 aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc
    14641 agtgctgctg caaataatac gtgtcttgcc atcggtactt cttcttccgt cagtagtccg
    14701 ccttcactca aaatttggat tgctcgccga gacgcgttaa attcgactgg taatgtcacc
    14761 aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga tattgattaa cgtagagttc
    14821 cagctaagca atacacctac cataatcaag gggaacgaaa tcatcgaacc aaagttagct
    14881 gctggaacaa gtgctgcacg aatcttcaat gggacataac ccgaagcatc ttgtaccgca
    14941 tgtccgcatt catgagctgc tacacctatc gcagcaactg aagtagactg tgcagttgct
    15001 tgagacaaac tcaacatctt attacctgaa ttgtagttat ctgtgagatc tccggcaatt
    15061 tgttgaacac cgacattatt tatctgttga ctttgtaata tatattgtgc tgcttgtgta
    15121 ccagtcacat tgtttttgct tctcacttga tcgtattttc ggaaagtact attgacgtac
    15181 gctgacgcaa ttccggaaat aactaatcca agaatgacca aaagaaatgt cggatctaaa
    15241 aaaccataca ttccataacc atacattttc gtttcactcc ttttcgttta ctttatttta
    15301 tcattagtag taggaaaaga atgtgaaaag accttgaata ttctttaatt ctttttcaca
    15361 aattccgatt tcagtttcat cggtccaaat ccttctacct tgcaatcgat attatgatcg
    15421 ccttctacta aacggatgtt cttgactttt gttccttgtt taatggcacc tgacgcacct
    15481 tttactttca agtctttaat cactgtcacg ctatcgccct ctaacagacg gtttccattt
    15541 gcatctaata ccactaatcc gtcttctatg acagtttctt gttcttcttt gttccattcg
    15601 ttcccgcatt ctgggcaaat caacaagcct cgatcttcat atgtgtactc tgagccacat
    15661 tctgggcaat ttggtaatgt catcttcttt ctcctttaaa atctttagtg agcactattg
    15721 taacggaaaa agaagatctt gaaaagatac atttgtgatt tttactacaa aaggcacgtt
    15781 ttcgaacgaa gtatcattct gtttttctgc tcttatacga ttggcatata tcttggatct
    15841 ctttatagat ttcctctact tctgctaaat cttttgcatt cgcaccactt gctaatggat
    15901 ggcctcctcc atgatggcgt ttagccaatt cattgatgat cggcccctta gaacgtaaac
    15961 gtacgcggta ataccctgaa ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa
    16021 tcactccagg aagtgaaacg atcgaagctg tctctgaatc atcgattcca tatttatcaa
    16081 gaatttcttg cggaaggatt attcttcctg caccattttc atccacctgg atattttgat
    16141 aaagataacc ggaaagttta gcgactttca tcggcatttg ttcaatctct ctgttcaaag
    16201 cacttgcatc aaatggataa tttctcaata tcgaagcaat ctctaatgta cgggacgtcg
    16261 tggagggata caaaaagcgc cctgtatctc cgacgattcc tgcataaagt aaccgtgcag
    16321 ccttaacgtt catctttaaa gattcaggaa acatcaaagc aaattctgca atcatctcac
    16381 tacagctact tgcttttgta tttacccaca caagatctcc ataagggtca tcattgggat
    16441 gatggtcgat tttgatcagt ttttctgcca actgatatct ttgatcactg attctgggtg
    16501 aattagctgt atcagtcacg atgactagcg ctccttggta gaagacatct tcaacttctt
    16561 gcatttctgc taaaaagcga agcccttcaa tcgtttcacc tacttgacgt atttcttttt
    16621 ctgggaaact ttcccgtaat agttcggcta atcctacttg tgatcctaat gcatcaggat
    16681 cagggcgcaa atgcctgtgg ataatgattt tatcgtattt ttttattgta tctactattt
    16741 cttgctgtac tgtcatttca ctcctgcttt ctaagaacgt tccattactt gacaaacaac
    16801 gattgctttg gaaacgatgg tgttttcaat aaacacttcg atatccagtt tagcggaacg
    16861 tctgccgatt tccaatacac gtggacgaat gtctaattcg ctttccaatt ggatcagccg
    16921 caaataatgt aggttcactt gttcaatcaa gatatttcgt ttttggttca tcaccattgt
    16981 tttttgtgcg acatcagaaa tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc
    17041 attgaccatt tgtggtgcta ctacgaattt gaacttaggg ttttccaaac gatttccttc
    17101 acggtcagta tcaatcgtat ggatatttcc agatatctga tccgaaatcg tatctgcaat
    17161 ctgcggttgt cgctggacaa gctgcatcgc tttcattaca tcttgccggg taatcaagcc
    17221 ttccaatgtc aagtcatcgg aaacgaccgg catgacttct agcccatccc agatcatttg
    17281 atggctaaca gaggcaatac tcattgtttt ctttacactg attggttctt tcgtcatcac
    17341 ccgatcgatg atctggtttg gcgatttccc taaaacatct tttgccgtaa caatccccat
    17401 caaacgatga tggcggttga cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg
    17461 ctgataatca gaaatcgtat ctgtctgctg caaataatga gtcttttcag ctggcatata
    17521 gatatcactg acaagcatga tatctttttt gatcaattgg tcgcttaacg ctcggttgat
    17581 catggttgcg actgtaaatg tatcataaga ggtgcgtaag atcggcattt caagatcatc
    17641 agctagcttt gcaatctcag cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc
    17701 atcttccaat gctaattttt ggacttccgc ccggttgccg acgatcatta gtgatcctgg
    17761 cgtaatatag cgttccatgg catgtttagt catcgcgccg atgacgaatt tattcaagac
    17821 cttgtctaat cctgccgccc cgcctaaaac gtccccttca atgatcctga cgacctcgcc
    17881 aaatgtcaaa cgttcaatat gtttttttaa tttgcgttcg atacggatgg tccctactcg
    17941 ttgaattgtt gaaactagtc cgacattttc tgcatctttg attgcacggt acgctgttcc
    18001 ttcactgaca ttcaattctt ttgcgatgct tcttacagaa attcgattgc caacaggtaa
    18061 actttcaata tattctaaaa tcaaatcatg ttttgtcgcc attttctgcc tacacttcca
    18121 gcgtttccga gacgctcatt atttttcctt cactatcttc cagtaactct acaaaagatt
    18181 cgggatcttg ttcaatgaca gggaaagtat tgtaatggat tggaactact ttcttggcac
    18241 gaattgtttg cgcagccttt gctgcatctt ctggccccat cgtatagtta tcgccaatcg
    18301 gcaagaaagc gacatcaata tcgaattctt ctgcaagcaa tcctagatca ctgaaataag
    18361 cagtatcccc tgcatgataa atcgtttttc cttctgcttg aataataatt ccgctaggct
    18421 cgcccatata ctgaacgata ccgtctactt catagcttga actatgctgt gcatgtacga
    18481 attttaccac gccgaatgga aacgcatgtt ttccaccgat attcataccg aatgaatcta
    18541 ctcctctgct gttcgcataa tcagcaatct caacaatact gataattgtg gcttgatttt
    18601 cttttgcaat aggaatcata tctcctatgt ggtcactatg accatgagta atcagcaacc
    18661 aatctgtctt tacttcttct gcagttaagt ctgtcaatgg atttcctgta ataaacgggt
    18721 caatcatcac gcgttggcca ttttccattt caattgttaa acaggaatgt ccatgatacg
    18781 taattttcat attttacacc tcgcgttttc tctgtatctt ttttattaaa aacgatttca
    18841 tcacttgcac atcaatgaaa aaatatagct gctaaatata ttttcattgg attgatagta
    18901 cagtatgtac aaagcaattg taacagataa tacaggatgc accaagtacc aagcagatat
    18961 aatctcaagt ttgaggtctc tgctctgctt agcttctctt ctcgtccctt aactaattaa
    19021 atcagcgtaa ataggatgga cgatcttttc taaatcggct acttttatcc gaaggcttct
    19081 cccgatttca ccgcttgaca caattacttc ttctttttct tttaaagaac tgtctgctaa
    19141 aaaacaataa tcaggatgtg cgagttttat tccaataggg gtgtttgctc cgtggggata
    19201 tcctgtatgc attaatacaa aatccattcc aggaaaacca actttacgat ctttcgtcac
    19261 ttgtctcgtt tttttgtaat ctaagtgatc gttcaaagga atcaatgcga tgatcacccc
    19321 agttttgttt cctttcacta cgagagtttt acatatcaaa gctggatcaa tccctctttt
    19381 ttgtagttct tctgtgaaag attcttctac cacatgacta aaatcaagtg gttgatagct
    19441 gatcttttgt tcttctaaat aacgttcgac ttttgtttga ctcatcattg attcactccc
    19501 tttattctat cgtttattat aaagcgaggg atgctgtatt caaaaatt
//
LOCUS       DAZKCY010000046        19491 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000046 DAZKCY010000000
VERSION     DAZKCY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.046"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..180)
                     /locus_tag="ACG8XC_001476"
     CDS             complement(<1..180)
                     /locus_tag="ACG8XC_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFP9191179.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKF"
     gene            complement(194..1444)
                     /locus_tag="ACG8XC_001477"
     CDS             complement(194..1444)
                     /locus_tag="ACG8XC_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFP9191180.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(1822..3273)
                     /locus_tag="ACG8XC_001478"
     CDS             complement(1822..3273)
                     /locus_tag="ACG8XC_001478"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP9191181.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(3594..4358)
                     /locus_tag="ACG8XC_001479"
     CDS             complement(3594..4358)
                     /locus_tag="ACG8XC_001479"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFP9191182.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(4386..5084)
                     /locus_tag="ACG8XC_001480"
     CDS             complement(4386..5084)
                     /locus_tag="ACG8XC_001480"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFP9191183.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5097..5876)
                     /locus_tag="ACG8XC_001481"
     CDS             complement(5097..5876)
                     /locus_tag="ACG8XC_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFP9191184.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            complement(5892..6836)
                     /locus_tag="ACG8XC_001482"
     CDS             complement(5892..6836)
                     /locus_tag="ACG8XC_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9191185.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6877..7656)
                     /locus_tag="ACG8XC_001483"
     CDS             complement(6877..7656)
                     /locus_tag="ACG8XC_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191186.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            complement(8040..10115)
                     /gene="glyS"
                     /locus_tag="ACG8XC_001484"
     CDS             complement(8040..10115)
                     /gene="glyS"
                     /locus_tag="ACG8XC_001484"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP9191187.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            complement(10117..11034)
                     /gene="glyQ"
                     /locus_tag="ACG8XC_001485"
     CDS             complement(10117..11034)
                     /gene="glyQ"
                     /locus_tag="ACG8XC_001485"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP9191188.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(11418..12230)
                     /gene="recO"
                     /locus_tag="ACG8XC_001486"
     CDS             complement(11418..12230)
                     /gene="recO"
                     /locus_tag="ACG8XC_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP9191189.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(12373..13272)
                     /gene="era"
                     /locus_tag="ACG8XC_001487"
     CDS             complement(12373..13272)
                     /gene="era"
                     /locus_tag="ACG8XC_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP9191190.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(13287..13682)
                     /locus_tag="ACG8XC_001488"
     CDS             complement(13287..13682)
                     /locus_tag="ACG8XC_001488"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9191191.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(13666..14142)
                     /gene="ybeY"
                     /locus_tag="ACG8XC_001489"
     CDS             complement(13666..14142)
                     /gene="ybeY"
                     /locus_tag="ACG8XC_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP9191192.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(14159..16351)
                     /locus_tag="ACG8XC_001490"
     CDS             complement(14159..16351)
                     /locus_tag="ACG8XC_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFP9191193.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            complement(16375..17346)
                     /locus_tag="ACG8XC_001491"
     CDS             complement(16375..17346)
                     /locus_tag="ACG8XC_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP9191194.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            18052..19431
                     /gene="brnQ"
                     /locus_tag="ACG8XC_001492"
     CDS             18052..19431
                     /gene="brnQ"
                     /locus_tag="ACG8XC_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP9191195.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         5355 a         4093 c         3428 g         6615 t
ORIGIN      
        1 aaacttttct tttctattta cttgtctcat gtagtacgtt ttttcgcctt ttgactcagc
       61 tgaatattct gtctgttctg ttataacaaa actctcatac ttctccatca gtggttttaa
      121 catcattaac tgttcataat gccccccaga ggaagccgca aaacatatct ttttgttcat
      181 attaacctct ctatcagtca cgctgaaaaa tatctctagt gtaaactttt tctttaatat
      241 catccaagca ggtatcatat cgattggcaa taatagaatc acacatcgcc ttaaactgct
      301 ctaaatcgtt gataacctta cttccgaaaa aagtctctcc atctttcagt gcaggctcat
      361 aaatcaccac agtcgcacct ttagctttta cgcgcttcat aactccctgg attgaactct
      421 gacgaaaatt atcactgttg ctcttcatgg tcagccggta tataccgatt aaaactggat
      481 tttccctgtt gacatcatac tgattatatt cagcgtagtt gtaatagccc gccatctgaa
      541 gaacacgatc tgcgatgaaa tccttacggg tacgattaga ttcaacgata gcctcaatca
      601 aatttccagg aacatctgca tagtttgcca acaactgctt ggtatctttt ggcaagcaat
      661 aacctccata tccaaaggat ggattgttgt agtgagttcc aatacgagga tcgagacaaa
      721 caccattgat tatctgctga gtattcagcc ccttcatttc agcataagtg tctaattcat
      781 tgaaatagct gactcgcagt gctaaatagg tatttgcaaa cagctttact gcttcggctt
      841 cagtgaatcc cataaaaaga gtatccacgt tctccttaat tgcacttttc tgcagaagtg
      901 ttgcaaaaac atgagcagca tttaacaagc gctcatcagt catatcagta ccaacaataa
      961 tacgagatgg atacagatta tcatataaag ccttgctctc gcgcagaaac tctggactaa
     1021 aaataatgtt cttactcccc gttgtctcac ggactgattc agtgtagcca acaggaattg
     1081 tagacttgat gaccatgatg gtatcaggat tcacgtccat cacaacctca ataactgact
     1141 caacagcaga agtatcgaaa tgctgagtac gactgtcata gttagtcggt gtagcaataa
     1201 caacgaaatc tgcggattta taagcgctcc ttgcatccaa agtagcagtt aagtcaagtt
     1261 ctttttctgt aagatacttt tcaatatatt catcctgaat tggagacttc cgtttattaa
     1321 tcagttcaat cttttcaggg ataatgtcta ccgcggttac ctggtgatgc tgcgccaata
     1381 gtgtggcgat actcaaacca acataaccgg tgccggctac tgcgatgttg tactttttct
     1441 tcacaatatc tcctctttcc tctcttaagt atggagaatg ttactcctac ggctattcat
     1501 catgtcaaaa taacacagct tagaatgttg ataaaataat tttccagaat gtgataaaat
     1561 ctctttcatt tataaaccac acatctaatt ttatctattc tccacaagac atgtagagta
     1621 ttctttaata acaagatacg accttactaa atacttcaaa aaattatcat atttaattca
     1681 taaatataaa tcttcatttt aaatgtatgc ccaaaaattt atctgtaatg cgtcatcaaa
     1741 atctagattc caaatcttta tcaattttta tccactcatt ctaccacttt agtaatattt
     1801 tctatttata acactttcgt cctacattga tccccccctc tgaagtacag tttttactgt
     1861 cttaaataaa attttaaaat ctaaccccat actccaattt tttatatatt ccgtgtcaag
     1921 ttttgctact tcttcaaaat cagtaatatc acttctacca cttacctgcc acattccagt
     1981 aattcctggc ttaatagcaa ttcttgcacg atgatgataa tcatattctt catactctga
     2041 tttaaggcat ggtcgaaatc ctataaggct catctttcct gttagaacgt tgaaaaattg
     2101 agggaattca tctaaagacg tggatctaat aaattgaccg attcctgtct tttttgttcc
     2161 atctggaagt attttatttc cgataattct tggatcaaac tccatcttaa acataagatt
     2221 attgctcatc ttattttctt tcattaattc tacttttctt tcttctgcat ccatatacat
     2281 acttctaaac ttatacatct tgaacggttt accattctga cctatacgtt cttgtgcaaa
     2341 gaaaattggg cctggtgaat taatataaat aagtggagca ataaagataa aaataattcc
     2401 cgttgcaata catccaacaa tacctgcaag aatatcaatt gttcgtttta taaatgcctg
     2461 ttgagttgtc ataacattga tacttgttgt aagtactgta tattctccta tacgaccaat
     2521 agattgtttt cccaaagcac tatctgatac ttttgcaaga ttcaaatgta cagtaaggcc
     2581 catttcagag catcgttcaa taaattcttt catacacgaa gtacttttat tcaaaataat
     2641 aaagacttca tctacccatc cctgacataa gtaatccgct gcatttttgg aatcagaaac
     2701 aactgtcact ccctcaacat cttcaccaat catgctttta tctatgataa tgacaccatt
     2761 tattttatac attttatagt tatgctcttt cacattacga ataactactt cagcaatact
     2821 actagatgta actataataa gtgatttgtt cccctcattt ttcattttat gtttcaagta
     2881 atgtttccat aagaggcgag ttatatatgt aattacaacg tataaacctc ccatcaagaa
     2941 aagaacgact ctagaataat catttccacc ctgaacacta atgagataca aacttcctaa
     3001 cagcaccaac ataatcgctt gctttacaga agaagcaaat tctatatagt accctctttt
     3061 taatacacct ctatatgctt caaacatgaa aataactaaa agatctgcca gttcaatgaa
     3121 gactgccatg ttttgataca ttggaatcac ataaggattc caatctacct ggcgtaagac
     3181 atacgataga taaaaagcaa gctggataca caataaatct aaaataataa aatcaatatg
     3241 tttaacccat ccccttgaac ttttcttgta cattcctttt tactcccctc tttttaataa
     3301 aataaattgt attacttgtt ttctctccta aatatctttc tgaaggatta ttgactcgac
     3361 aaattggctt gtggatattc ttatatatag aacatccgta agccaactta aaactcctta
     3421 acaaaatttt tttactcata ttgtagatca aaacaagtat ccaagaaatc atattttatt
     3481 tcttttttct gcttactcaa caacagctaa aagaaaaatc atcataaaca aattcaacta
     3541 agccaacgat ccccttgtga aatcaagttt tttcttgatt tacgaataaa gagctaaaat
     3601 aaaccaaact tcttcttttt tacctcttca tagactggat aactgatttc atccccgttg
     3661 atcagatcct ttgcgacttg tttcatctgt actacttttt ccttaccaaa gtcctttgca
     3721 atctgctcat agcactcttt taagtaaaag gtgcgcttag agaccccatg ggcatcagaa
     3781 gcagccatct gcacgagatt atgtttgacc atcgttttgg ctgttttctg gatgtctttg
     3841 ccaaataccc caacataact tggcgcagtg agttgggcca aacatcccat atctaggaac
     3901 ggaagcaaat gatttgggtc ttcgcgaaaa tgcacgttgc gttctggatg gacgattact
     3961 ggtgtctttc ctagttctaa caactcaaaa aaaagccgtt ctgcatataa cggcacttcc
     4021 agtgtcggaa attcgatcag aagatatgta tcatctaaat ctgtaaataa tatttcatct
     4081 cggtggatgt cttcgatgag cgttccggta atacgtactt cttgtccttc caataacgtc
     4141 aaaggcaact gtcttttctc taattcggct tgtaacgaag cgactagaga aatgacttgt
     4201 gatttttcat tgctgtattt tccattattg tggtgtggtg tgcataaaat atgcgtgatg
     4261 ccttgctgaa tcgctttttc agccattgca atacttgctt ctaagttttc tgccccgtca
     4321 tcgattcctg gtaggatatg gcaatgtaga tcgatcattt ctcttgtctc ctctcttctt
     4381 attccttaat ttccgtagta ataaccagca tctttgctat gttctgctcc gttgtacaca
     4441 acacctaaga ctcgtgcttg taccatctcc agcagttctt ttgctttagt caaggattct
     4501 ttacgtgaca tattttcacg tacgaccaat aaagtgccat ccgtttttaa agacatgatc
     4561 tgtgcatctg ttacggctac tactggtggc atatcgaata tcaccacgtc aaataactgt
     4621 cttgcttcgg ataggatctg atccattctt ggcgagctta acaattctga tgggttgggt
     4681 ggcttaggtc cactaggcaa aatcgacaag ttctcgactg gcgttcgttg gatcacatct
     4741 gctacacttc ctgatgaact aagtgctgta cttaatccac tggcattgtt cagctggaat
     4801 gtcttataga cgacgggttt acgtaaatct gcatcgacta gtagaactcg ttgacctgat
     4861 ttagcaaaaa cgactgcaat gtttgctgca gtggtcgatt tcccttctcc aggtcctgat
     4921 gaagtcacaa caatcgtttt aatttgctga ccagctgcgg aggcaaactg gatattcgtc
     4981 cgaatcgtac gatattgttc agcgattggt gaagaggggt ttgtgagtgt gaccaaactg
     5041 actgcatgag tttgggctgc tttttgtttc tgtgttcgtg ccatttttct ttcctcctag
     5101 actcttgagc ggcttctgcg tgatggcgct gttttatcgt ttgtagcttt tggttgtgtc
     5161 ggagctggtc cttttttacg gataccagca ttcaattctt tcgctgacat ttctggtaca
     5221 gtccctaaga tcgggaagcc caatgtatcc gtcacatatt tttcatcttt tactgtccgg
     5281 tcgagtaatt caaataagaa agctagacct acgcctacca tcattcctaa cactaagcca
     5341 atcactagat tcaatttatt atttggcgaa acaggtgtcg tattggcaac tgcacctgag
     5401 atgatcgaga ttttatcgac attcatcacg tctttggcgt tctcttggaa tacttgtgac
     5461 gttgtattag cgatttgttg agccgtcact gcatttgtgc ttgttgcttg gatcgagaac
     5521 atttgtgaat tttgtgactg attgacggag atggcatctt tgatttgtcc agcagtcatt
     5581 tttacattgt cttctgtttc taaacgatct ttgacttcat ttagtactag atcactgacg
     5641 atcatatcct tatacgtatt gatcatttgc aggttcgtat tgacatcatt gacatttgca
     5701 gtatctgatt gcggcagtgt cacgatcaat tgggcttgtg agctgtattt tggcgtcaag
     5761 atgaaaaacg tcacgatgcc tgagatggcc aagccagcaa acattgagat gaggattgtt
     5821 atcacgtgtt tgcgtaatgt ttcaaaaatt tctttcaagc tgattgtctc ttccattttt
     5881 tctccacttt cctatttatt ctctagattc atttgatctt ttaattcttt tttcacacga
     5941 tcaagttctt gatcgctgac tttttggtat gagatattgt cttgcatgaa gccttctcct
     6001 tgcagctgat cttgcttgat cgtaccaaat gcatcccgat aatctaaagc aatctttttc
     6061 atttcatcaa agctcatgtc tgttttcata ttcgactcta gagcattcaa aacggattga
     6121 tagttgctta caccgttcag actcaacact tttttcgcta caccttctac gatctttctt
     6181 tgacgttctt gtctgccgta gtcaccattt ggatcttcat aacgcatacg agaataagac
     6241 aatgcctgat ccccgttcaa tgtgatttta ccgatcgtaa aatcatagcc atcttgtgag
     6301 aaggtgaggt cattattgac ttcgatacca ccaactgcat caacgagttc ttttagacct
     6361 ttcatattga ttgaaacata gtgatcgatt gggatgtcta catatttttg tacggtatcc
     6421 atcgccatgg cagctccgcc gaatgcgtaa gcgtggttga tcttgtcttt tgtgtcatgt
     6481 ccaacgatgt cgacatacgt atcacgagga atgctgacaa tcgttgtctg tttgtcttca
     6541 ggactgactg tggcaaccat catcgtatct gatcttcctt gatcgacccg acctaaatct
     6601 cccgtatcaa tccccatcaa tagaacagag aaagattctt gtttgtccag atcaactggt
     6661 tttgttcgtt ctccttgggt tcgttccact gattcgtaag ttttttcaat agaatgtgag
     6721 agatctagat ataattttgc tcctactcct acgattgcta atactaatac gacaagaatt
     6781 gctaaaataa ttagaaatat ctttttccct cttgatcttc ttcttcgttt tgccatagtt
     6841 tcctccgttt gctagccttg cttgaattct cgcttattaa tagctcgtat tgctatagcc
     6901 catttgctga gcgactgttt ctgcttttga cgcgtatgcg gctcttattt tttcattccg
     6961 gatctgttcg atgagactga gtgcggtcaa atattgatcg taagtagcag tagctgttac
     7021 ttgaccgtct ttcagcatcg tatcgaatag ctgatctagt tgcgtgactt gtgcaatttg
     7081 ggtatcagcg aatcctagat attgcataac gaccgttttc caagcactat ccttaaaaaa
     7141 gctcatattt tcacgtacat gggcgacatc tgcacttgcc aacttttctt tgatgatgac
     7201 atcatccaca gcttgctgcc agtttgatac gtttttagta aataatttat tgacagccgt
     7261 ttggatcttc agttttgatt ctgcatcctc taactgtgtt aagactgctt tcttttcttc
     7321 ttggatcgca gcagcttctt taggtaattc tgattccttg atgtcataat catctgctga
     7381 aagtttgata gaagatagtt tatgtgacaa atttgaaact gtttcttctg ttatttcagg
     7441 atttaggaaa attttttctt ttgtactgta cagcttatct acttcttttg ataaagaggt
     7501 cacggtgtct ttttgattgg tgatggcaga ttctgcgtaa ttgatccgtt gatgatgttg
     7561 ccgctgatta ttgagatggt atccaaacaa gcaaatcaca atagcgcctg tgcctaccgc
     7621 cacagtagcc aataacttga cgaacggctt ttccatagat tcactcctaa atattgatga
     7681 tagataaata aagatgtgat tcattcacat cagaatgagc gctttttatt ttaaaagcat
     7741 aattaaacta atatattcat agaaatattt ataaacaggt tttccctata taatcatacc
     7801 acaaacagtt ttcttgacta gggaaattcc taatttaaaa gtaaataaaa cagtaacaag
     7861 attattatta atctttttca tttttatttc ctcacttttc atgagcgttt tgaaaattct
     7921 agcaaagaat aaaataacaa aaattattaa aatagaatta ataacagctt ttatatttat
     7981 aacaaaaaaa cagagacaca gcacgtttcg tgcatgctct ctgtttttca aacaaaagac
     8041 tattttgtga tgatttgatc caagcttgct aatgacaaag ccatggcagt tatttgacta
     8101 agttgtgtta gtcggttctt acgtactgct ggttcatcag ccatgaccat cgtttgatca
     8161 aaataatttt cgatcagtgg acgaagagcg acaagctggt cgtagttttc gtggatcgtt
     8221 tggtcagcaa aacttgcttt cattgcttct actgctttgt gtaattcttt ctctgcgtcg
     8281 ttttcgaaca gctcagggtc tacttctgat tgtttttcaa gctgcgcttt tttcgctaag
     8341 ttcacgacac gtgtcaatgc ttccattgaa ggtttgaagt cttcatctgc aagatgttgt
     8401 ttgaacaatt gtgctgctgc aaatactttt gtcagatctt tttgttctgc attcaaaaca
     8461 gcttcgatga catcatgacg gatattcttc gttgtcaata attggcgcag acggcctttg
     8521 ataaagtcga tcacctgttg ttgtcccgct gacaagccaa tgccaaaacg atccgtatct
     8581 ttactgataa cttcactaat cgtttcttgc agaacagaca aaggaaaggc ccattttttc
     8641 gattcgatga ttcgaacgac accatatgct tgacggcgca aagcatatgg atcgtttgag
     8701 ccacttggga tcatcccaac tgcaaagaaa gaaaagatac tatctaattt atcagcaatc
     8761 gctaaaacag aacctactgt agtctcaggt aaatctcctt cactggatat aggcatgtaa
     8821 tgttcacgga tcgctgttgc tacggctggt ttctcgcctt taagcagagc atatttttct
     8881 cccatgatcc cttgtagttc agggaattct cctaccatgt tggttactag atcaaattta
     8941 tagatttcag cagcgcgttg cagatccatc aattcttcct cagatagtcc tacttgttcg
     9001 cctatgatgc gagcgatgac ttgtacccgt tgcattttct catagatgct accgattttt
     9061 tcatggaaag taacagtttt taatttttca acgtattggt cgattgaagc tttcttgtct
     9121 tcattataaa agaattccgc atcttctaaa cgagcagtca atactttttg attgcctcgt
     9181 accacatttt ccaattttac ccgatcgcca ttacgagcag aaataaaatg aggaagcaac
     9241 ataccttgat cgttccgtac ttcgaaataa cgttgatgtt ctttcataga agtgactagt
     9301 acttcttcag gaacagatag atatttttct tcgaagtcgc cagaaaagac agttggatat
     9361 tcgactagat tgttgacttc ttctagtaaa tcttcatcta gatcaatgat ccagtgattt
     9421 ttttctgcta attcgtttgc ttgtgtgatg atcatttctt ttcttttttg cggtgaagca
     9481 ataacaaatt gttcattcaa tttttcttcg tattcttttg catgcgtaaa tgtcacatct
     9541 tcgcctaaga aacgatgacc tcttgaaact ctgtctgttt tgacatccaa gatctcaaac
     9601 gggattattt cgtcatctaa caatgccacg atccagtgaa tagggcgaat atattcaaaa
     9661 tcgtattctg cccagtgcat cgttactggg aaagtgaggc ttttaatgac atctgctaaa
     9721 cctgtcaata cttcttttgc ttctttgccg tgagtgtatt tcgttacata gacatattcc
     9781 acgcctttta attctctaaa agtgatagca tctgtcgtca gaccttgtcc gcggacaaat
     9841 ccttcagctg ctttcgtcca gtttccatct gcgtccaaag caattttttt ggctggtcct
     9901 ttgacttctt cttcgctgtc tgcttgacgt tctggaatgt ttgttacttt gattgctaag
     9961 cggcgcggtg tagaaaacgt ttcgatcgta tcataagtca attggttttc atctaaaaat
    10021 tttgctgttt tttcttctaa ttgtttcatg cttggtgtga ctacatgagc aggcatttct
    10081 tccagcccga tttcaagtaa taagtctttt gccatcttat tcagcctcct tcacgttttc
    10141 tggatttgct gtgtttttat tcaataaagg gaatcctaat ttttcgcgtt ctgccacaaa
    10201 gattttggct acagcacgag ccatgttgcg gatacgggaa agataacctg cacgctctgt
    10261 tacggatact gcaccgcgag catctaataa attgaatgta tggctacatt tcaaaatata
    10321 atcatacgct ggatgaacaa gattttcatc gatacagcgt ttagcttctt tttcaaaacg
    10381 atcaaaattg cttagtagca ggtcttggtc gcttacttca aaagaatatt tagaatgttc
    10441 atattctggt tgtttgaaga tctcaccata tttcacgcca tcagcccatt ccagatcata
    10501 aacactttca acttcttgga tataagaagc aagacgttct agtccataag tgatctcaga
    10561 agtaactggt ttacaaggta gtcctccgac ttgctggaag tacgtgaatt gtgtgatctc
    10621 catcccatca agccatactt cccagcccac accggcacaa cccatcgatg ggttctccca
    10681 gttgtcttca acaaagcgaa tatcgtgttc taatggatca attcctaaaa gacgcaagct
    10741 ttctaaataa agctcttgga tattttcagg tgaaggtttc attacgactt gaaattgatg
    10801 atgctgatac aaacggtttg ggttttctcc ataacgcccg tcagctggtc ggcgggatgg
    10861 ctctacataa gcagcattcc atggttccgg tccgattgcc cgtaagaatg tatatgggct
    10921 catcgtacct gctccttttt ctgtatcata agcttgcatc agcatgcagc cgtttgatga
    10981 ccaaaatttt tgtaatgtta aaatcatatc ttggacggtc aattttttac tcatctgttt
    11041 tgcccctttc aatttttaag cgttgctgtc aataagaata caggaaagaa aacgacaaaa
    11101 agtccctatg tctgattaca gacataggga cgaataattt cgcggttcca ccctacttcc
    11161 gaataaagat tcggcagctt catttatcgt actcgcaagt gcctcttcaa aaatcgtttg
    11221 ggtccggctt ccactctccc gaactcgcta aaccacgtgt tttttactga tcttggtcaa
    11281 tgtacgtatt cattttgcag ttttctggtt tgtataatag atcctctcag tgaacgatct
    11341 atttaatcta cggttaataa tagacggaag tccctgtatt tgtcaaatag ttttgatttt
    11401 tttgttgtat ctttcattta ttcttctttt tcaggtatct ctttttcagg catatttttc
    11461 tctggcgtct ttaatacgtg tgcccaagac ttcatttgat caatgaactt tttgcttttt
    11521 agatgaatac cgacatattc atcatacaac tggtcaatga ggtcacggat tgcctgcttc
    11581 gtttcttctt tcaagtggat tccactgatc ttatcataag aaatagccga aaacattcga
    11641 acgaaataaa cagcgcgagg atctgcatga taacggtgtt cgtccattgg ccagtgtttc
    11701 tcgcatatca caccgccata tttggaagaa tagtcaaatt ttccttttgt ttccccgcat
    11761 acgacacagt gcgtccaaat tggcgcgatt ccgaagcgtt gcagtatttg cacttcaaag
    11821 atattcgtga tgatttctgc atcatctcct tggtccatcc gctgcagagc ttgttgcgta
    11881 aattggaata aatgcgggtc atattgatga tcttcgattg ctgcatctac tagatttagc
    11941 aaatacgtac cataaccgct caaaaagata tcttcttgga tgttccgaaa cgcatggacg
    12001 tctttgacac tgcttaaaaa cgacaaacct tcacttcgaa aattaccgat atagactgct
    12061 tcggtaaagg ggagcaaagc cggagctaat ggctgatttt gccgatggat tcctttgata
    12121 taaaacatca ttttgccaga agattccgta aagattttga ctaatttatc tttttctttg
    12181 taattacggc tagataaaat caagcctttc gattctacaa gctggctcat attctccctc
    12241 ctaaaaaata aatctggaaa agcagattct gccgatccag attatttatg tttcgatctt
    12301 ccgctgtttt tctcaacgta cggattttta tccagtttat ttctatacct aacctataag
    12361 aagtccagaa tatcagtact cgtcttttcg gtagccgaag tcttgcagat agactttttt
    12421 gtctcgccag tctttttgga ctttgaccca aagttctaga aatactttgt ctcctaaaag
    12481 attttcgata tcctttcttg ctttcgttcc tacttgcttc agcatttttc cgcctttgcc
    12541 gataatgatt cctttttggc tatctctttc gacgatgatc gtggcctgga tatggatttt
    12601 atcattctcg tttcgcttca tcgaatcaac aacgactgcg acagaatgag gcacttcgtc
    12661 tcgtgttaaa aacaagactt tctcacggat caattcagaa acgatgaagt attcaggatg
    12721 atcagtcact tgatcatctg gaaaatattg tgggccctct ggcatttcac tgactaatac
    12781 atccattaag cgttcgacat tgtttccttc tgtagcagag atcggaatca cttgtgcaaa
    12841 aggcatctgc gtcgaataat cttcgatgat tcccagcaac tcgtctggat ggatcttatc
    12901 gattttattg ataatcagat aaacaggaga atgcacgttt tttagacgtt caataataaa
    12961 atcgtctccc cggccacgtt tttgatcagc actgatcata aataagactg catccacttc
    13021 tttcaacgca ctgtaagctg cttctaccat aaaatctcct agacgatgct ttggtttatg
    13081 gattcctggt gtatcgataa aaatcagctg agcttctggt gtagtatata ctccttggat
    13141 tttatttcta gttgtctgtg ccttgtcact cataatggca attttttgac ccacgattcg
    13201 gttcaataat gtggattttc ctacattcgg gcggccgacg attgcgacaa accctgattt
    13261 atgttctggc atatacgttc tcctttttaa ttaaacaaca aatgatatat tttagggaca
    13321 aataaaatca acccaatgat gacagcaaaa caagcggtca ctaaaactgc tccagctgcc
    13381 atatctttga tttttttccc aatcggatga aaatgaaaat ccgtaaacat gtccactaca
    13441 ttttcaaaaa cagtattgat gatctctact atccaaacga gaaaaacagc taaaagcagc
    13501 cataaccact ctatccgatc aagctggaaa atcacgccca taaataaagc aaggataccg
    13561 aacgcaacat gtttcctcat attgcgttct tcctcaaaaa cggttttgac tccttggaca
    13621 gcaaattcca cagacatcag gaaatgtttg tttttctcta cgtttttatc ttttgagtcc
    13681 ataggcatcc aagatctctt tctgtaaacc aaacatttct ttctcatctt ctggtgtcat
    13741 atggtcatac ccgttgatat gcaaaaatcc atgtaccgct aaaaagccaa gttcccgatc
    13801 atatgaatgg ccgtaagctt ctgcttgttc agcagcacgt tcaatagaga tcatgatgtc
    13861 tcctagtaca cgaggtaatg gattctctcc atcttcaaaa atcactggga tttcgtcttc
    13921 gccttcttct tccagagcaa aactgatgac gtctgtcggt gcatcttttc cacggtaatc
    13981 acgattgatt acttgaattg ccgcattgtc catgaaagta acagacattt ctgtttcctc
    14041 tggcagcttt aaataagatg ctgcaaattg cagcaagtca tcgatttctt tcattttctc
    14101 ttctgagact tggtttgttt catccataaa ggtaatatcc attatacatc gcttctttct
    14161 attcttcttg tacttccttt atcttctcaa cttctgtttt cattttagga tatttgatcc
    14221 gagaatggaa ggtgctactt aaaccgctga ttaatgattt ttccactttt cggatttctt
    14281 tcaaggtcag tccactatcg tctaattgac catcggaaat acgctcttcg ataagattat
    14341 ggacaaaact ttcgattttt tcgctcgtcg gatgatccat tgcccgtact gcagcctcac
    14401 aactgtcagc aatattgacg atccctgctt cacgactttg cggttttggt ccaggataac
    14461 ggaattgctc ttctgttact tccggattgc gttctttcgc tttgatgtag aaataacgca
    14521 ttagcgtggt tccatgatgc tgtttgcaaa tatcgatcac catttgcggc atattataat
    14581 cttccaatat tttggctcca tctgtcacat gtccaaagat aatatgttta ctgtcttctg
    14641 gcaataagaa attgtgcggg ttttccgctc ctgaaggcaa gttttcaacg aaaaagctag
    14701 catgtttgat cttgccgata tcatgataat agcaagctac tcgagttagc aaagaacgtc
    14761 cgccgatttc tgctacagcg ttagcactta gattagctac catcatacta tgatgataag
    14821 tacctggcgc ttcttccagc aactgtttca ataaaggatg atttggattg ctgagttcat
    14881 ttaatacaat tacactgtca tcattgacca tcaattcaat atatgggtgt agaccgatcg
    14941 tcagcaagaa tgagaagagt gcaccagtga acccgcagac gagcattaac caagtcgttg
    15001 catcaccaaa gctcattcct tgatagacga tcaaaatgac atccatcatc acaggaaaaa
    15061 taatgaccca catggcagca gataatcctt gttctgagat ccgtttgcgt ctgacaatcg
    15121 ttgccatgag cccagagaaa acataagaca tcaagataat cgttagaaag ttcgtgccaa
    15181 tcgaatcata aaaaatgaaa agtgcggata ctgcttggaa aataccagcc atgatccctg
    15241 ctcttcgatt caagaaaaag cttaaaacaa gtggtgcaaa agcagcggga taaaacagcg
    15301 ggatataagt agcttgttcc gtttggaata attggaaaaa tttcatcagc actatgctga
    15361 ctgacatagc tgtcacatag aaaagcacgt attttgttct ttctcctgca ttttggaatt
    15421 gaagtgaatt ataaatcagt acgccgattt gaagcaaaac agctaaaacc atggcaatta
    15481 gcggaaaaat cgaagttgtt tgattagtca gtcctagtaa ttccaatttt ttcatagctg
    15541 aagcatcgat ttgattacct tcacgaacaa tgatttctcc ttggaagatc atcactggtt
    15601 gtacagcttc tcgtgcggat tgtttcaatt cttctgtctt tttctcattc aatacgtcat
    15661 tcactacgat accttgatcc actaagtagc gaatcatttg ttgttgtgct ggtgtgacat
    15721 tcaaatattg gatttgctct tctgcttctt gtttaaaagc ttccaattca ctttcacgaa
    15781 cttgtttgct catctgttcg tcaatgagct tcagactctc atccctcaca gtatgaatct
    15841 cagtttcagt catttcaaat atcgtcgaat aaaaattgtt cggtagtttt tgataaaaca
    15901 caacattttc tgcattcaca ttttcaaaat ttttcttcag cgcggcgatc cgctcttctg
    15961 tagtaggctt tgggatattt tcatcttcct ttgccttatc aatcttttcg tcataagatt
    16021 tgttgatggc atcattcgtc ttatcgatca attcaaatag ttgcttgatc cgattgttct
    16081 ggtcatctgc aagatctttt tggtaagtgt attctggagt gactgcttct gctgccagtt
    16141 ttcttttttg ttcggtttct tccgtgttct cgatcgtttt gttcgcacgg atactttctt
    16201 ctgctacttg gccttctctg aaatcattgg ccttttgttt gacactacta aatgtaacgc
    16261 cgcagataat gataaaaaac aagaacagca ggaaaggcaa tgctgccttt ccaagttttt
    16321 cttgtagttt ctgcagtgtt ttattcaaca tgtgcttatc ctcctaagca aagcttattc
    16381 ttgatggaga tctgcctttt cataagcttt gatgatttcg gcaactacag ggtgccgaac
    16441 aacgtcactt gcttcaaaat taacaaaatc gattttcttg atttgcttta atgttcgttc
    16501 tgcatgaaca agaccacttg tcgtccctcg aggcaagtct atctgacttg tatccccatt
    16561 gacaatcatt tttgattgat aaccgagacg ggtcaagaac attttcatct gagcgactgt
    16621 cgtgttttgt gcttcgtcca agataacgaa tgcatcatcc aatgttcgcc cccgcatgta
    16681 agctaaaggt gcgatttcga ttacgcctcg ttccatcaat cggttcgtat gatccaatcc
    16741 aaaaatttgg tataaagcat cataaacagg ccgcagataa ggatccactt tttcttttag
    16801 atccccggga agaaagccta gattttcacc tgcttccaca gcaggccgcg tcaaaatgat
    16861 tttttgcact tcaccttttt tcaaggcagc gattgctaat actactgcaa gaaaagtttt
    16921 tcctgtcccg gctggtccta cgccaaatac gatatcatgt ttggctaccg attcgacata
    16981 tttcttttgg ccgctgtttt ttacccgtat aggttttcca ttacggtctt tgacgatctc
    17041 ttcctcatac atctcaacaa aataatctaa tgtacctttt tgccccattt tcaatgccgt
    17101 cacaacatcg ggcgtactta cgtggattcc tcgtttgatc aattcctgta atgtccgaat
    17161 gactgaggca gccaaggaaa tctgggattg ctcaccaatg atctgaatga tttctccacg
    17221 tgtatggatg gttgtttccg ttgtttcctc aatcagtttt atatgcttgt cgtgtgcacc
    17281 taaaagcatg ctgacatcat cagcagccgt caatctaatc tctaaagaac tagactcttc
    17341 cgtcaaaaat agacatcccc tttttttcga ttaattcttt cctattttac catactcaga
    17401 agaaactata tagcaaataa gaaaaggttc taaaagagtt gcttaaccat aagtaccaag
    17461 aaacgttaaa aaactagccg cttccattct ctaaaggtct cgtttgaata aaaaaagctg
    17521 atcatgtaag ctatgcataa gatagaggac aagtattccg tctaacttag caagtattct
    17581 ctgggtattt ttcttgtttc aaaaaaagaa aacaaaatat ttgaaatatt cttgcatttc
    17641 aaaaaaaaca ggtgtattat aacgtcaaca aataaatata cagcaaagcg tcgaggaaaa
    17701 cagtaggcta ttcagtgatt tattcccagt gagtcatgta agtgagaaat gacaataaat
    17761 cgaatagacg aacatcattt ccgagtcaat gtcgaaaacg tagatcattg tggctgcctc
    17821 cattatcagg ctagcggata actttatcga aaagcgtccg tgaatgaaga agctttttta
    17881 agcgaaaaaa ggtggtaccg ttctagaatc tagagccctt ttcccaagtg gaaaagggct
    17941 ttttttatat tttaaggtgg tggttttcat ggaatctgat attagtggta tttctagttt
    18001 ctgcaacgtt tattttgtta ctcaaaatga taattattgg aggaaaaatt tatggaagaa
    18061 acaaaaactt attcatggaa gcaattattg atggtcagtt cacttatctt cgggatgttc
    18121 ttcgggtcag gtaatctgat ctttcctgta cacttaggtc aaatggctgg atcaaactgg
    18181 atcagcgctg gtgtaggatt tgccatctca ggcgctcttt tcccattatt agctatctta
    18241 gctgtagtag tgacaaaaag tgatggtctg tatgacttag ctaaacctgt cggaaaatgg
    18301 tatgctgcct tgttcttgat ccttgttcat ttaacgatcg gaccattctt tggtacaccg
    18361 cggacagcag caactgcttt tgaaatggct gctaaaccct ttttgcctga aaaatatgat
    18421 tcattaggta tgtttttatt ctctgctctt ttctttttac ttgcttattt ccttactgta
    18481 catccaacac gtttggtcaa atatgtcgga aagtatttaa actccttgtt tcttgcatta
    18541 ttagctatca tttttgtcgt ttctttattc caacccatgg ggaatctaaa ccaatctgct
    18601 gcagattctt atcagacgaa cgctttgaca aatggcgttt tagaaggata caacacggtt
    18661 gatgcagtag ctttacttgc tttaagtgtc acttttgtcc atgcagttcg tgatcttggt
    18721 tttaaagata aaaaagtcac agaattaaca gcaaaagctg gtagcctgag cgttgcgtta
    18781 gaagtcatga tttattttgg tctggtcctt ctcggtgcga tgagtttgaa taagatgtct
    18841 ttatcagaaa atggcggaac tgcactttct cagatcacta atttttattt aggtaacttc
    18901 ggtgctagct tcttaggggt aatggtcaca ttaggtgtct tcactactgc tatgggactc
    18961 gttgtttctt ttgcacaaga tttccataaa ctgttcccta aagtcagtta tatgacatgg
    19021 ctgcggttaa cgacttttgt ctcttttgtc gttgcgaatg ccggattgga taatatcatc
    19081 caatggtctt taccagtatt gatgcttttg tatccattgt cacttgcgtt aatcttactt
    19141 tccttaacag caaaattctt ccaaaagagt ccatttgtct atcaagtaac gatgttcttt
    19201 tctgccgttc cagccgtatt ggatatgttg gcaaattcgc ctgcattagt gagtcagcaa
    19261 actgtcgtag caagcttact aggattttat catcattatg ttccttttgc tgcattaggt
    19321 cttggctggg tcgtcccaac attgcttggt tacgctggca gcttgctctt ttattatgta
    19381 tatcgcgtat caggatataa acaagaagca agtgagttac cagaagaata accaaatcaa
    19441 acacaattta acataagaga tattaaacag gaaaaaaaca gctagaagaa a
//
LOCUS       DAZKCY010000047        18534 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000047 DAZKCY010000000
VERSION     DAZKCY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.047"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..119)
                     /locus_tag="ACG8XC_001493"
     CDS             complement(<1..119)
                     /locus_tag="ACG8XC_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9191196.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            298..1959
                     /locus_tag="ACG8XC_001494"
     CDS             298..1959
                     /locus_tag="ACG8XC_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9191197.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2029..2376)
                     /locus_tag="ACG8XC_001495"
     CDS             complement(2029..2376)
                     /locus_tag="ACG8XC_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFP9191198.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2460..3401
                     /gene="opp3b"
                     /locus_tag="ACG8XC_001496"
     CDS             2460..3401
                     /gene="opp3b"
                     /locus_tag="ACG8XC_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP9191199.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3402..4445
                     /locus_tag="ACG8XC_001497"
     CDS             3402..4445
                     /locus_tag="ACG8XC_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9191200.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4451..5521
                     /locus_tag="ACG8XC_001498"
     CDS             4451..5521
                     /locus_tag="ACG8XC_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9191201.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5511..6464
                     /locus_tag="ACG8XC_001499"
     CDS             5511..6464
                     /locus_tag="ACG8XC_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9191202.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6476..7486
                     /locus_tag="ACG8XC_001500"
                     /pseudo
     CDS             6476..7486
                     /locus_tag="ACG8XC_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
     gene            complement(7771..8592)
                     /locus_tag="ACG8XC_001501"
     CDS             complement(7771..8592)
                     /locus_tag="ACG8XC_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP9191203.1"
                     /translation="MNRFASKEEEKLYYEHEASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8689..8967)
                     /locus_tag="ACG8XC_001502"
     CDS             complement(8689..8967)
                     /locus_tag="ACG8XC_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191204.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9235..11244
                     /gene="metG"
                     /locus_tag="ACG8XC_001503"
     CDS             9235..11244
                     /gene="metG"
                     /locus_tag="ACG8XC_001503"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP9191205.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11723..12310)
                     /locus_tag="ACG8XC_001504"
     CDS             complement(11723..12310)
                     /locus_tag="ACG8XC_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191206.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12485..13807
                     /locus_tag="ACG8XC_001505"
     CDS             12485..13807
                     /locus_tag="ACG8XC_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9191207.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13962..14738
                     /locus_tag="ACG8XC_001506"
     CDS             13962..14738
                     /locus_tag="ACG8XC_001506"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP9191208.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14731..15300
                     /gene="rnmV"
                     /locus_tag="ACG8XC_001507"
     CDS             14731..15300
                     /gene="rnmV"
                     /locus_tag="ACG8XC_001507"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFP9191209.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15293..16177
                     /gene="rsmA"
                     /locus_tag="ACG8XC_001508"
     CDS             15293..16177
                     /gene="rsmA"
                     /locus_tag="ACG8XC_001508"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP9191210.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16628..17257
                     /locus_tag="ACG8XC_001509"
     CDS             16628..17257
                     /locus_tag="ACG8XC_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191211.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17381..18490)
                     /locus_tag="ACG8XC_001510"
     CDS             complement(17381..18490)
                     /locus_tag="ACG8XC_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9191212.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         6056 a         3338 c         3773 g         5367 t
ORIGIN      
        1 cagataaggg actgtgacaa gacttttgtc acagtttctt tttgtttatt cttttttatc
       61 tttgtatggc aataatagac aatataacga aaattattta atggcttttg gaagaccatg
      121 gaaaaccttg tacaaagcta ttttttctga aaaattcctt gaaacctcac gaaaaaatca
      181 ttattttatg aaaaagacaa aaaaaagtgt tgatttttta ataattattc attaaactct
      241 taaacaactt cagaaaatat taattattct gaaaattcca ataaaggggt acgtaaaatg
      301 aaaaagaaat ttacatttgg tttggttgcg gtatgcggcc ttgtacttgc tggatgttat
      361 ggcggcagtt cagccacaag cagtagttca gcaaaaagcg gttcagcaga tggaggaggc
      421 gtatttaact tagtcgtgcc tcaagaaatg cctacagctg acttatcagt tgcaacagat
      481 acaatcagtt ttacagcatt gaataacgtt tatgaaggaa tttaccgtct tgatgaagac
      541 agcaaaccac agccggcagg agctagcgag ttggcagaag tcagtgaaga cggattaact
      601 tacaaaatca aattacgaga agacgcaaaa tggtcaaatg gtgatcctgt tacagcagcg
      661 gattacgttt atggctggca acgaacagta gacccagcta cagcttctga atatgcttac
      721 ctgtatgcgc cagttgaaaa tgcagaagac attacagctg gaaagaaaga taaatcagaa
      781 ctagggatca aagcagttgg tgactatgaa ttagaaatca aattgacgaa acaaacacca
      841 tatttccaat atctgcttgc tttcccatca tttttcccac aaaaacaatc cgtagtggaa
      901 gaaaatgggg atgcctatgc atctgcaagt gataaagctg tttataatgg accattcaca
      961 ttagaagatt ttgatggacc aggaacagat acagaatgga cttataagaa aaatgatcat
     1021 tactgggaca aagatacagt aaaactttct gaaatcaaag tcagtgtagt aaaagaatct
     1081 tctactgcat tgaacttatt caaagatggc caagcagatg acgtgatctt gtctggtgag
     1141 ttagcacagc aaaacgcaaa cgatccagct tatacttctg ttaaagaagc gcggacaagc
     1201 tatatcgaat tcaatcaacg tgaaaagaat tcaccattca acaatgtgaa cctaagaaaa
     1261 gctatttctt attctatcga tcgtgaagct ttagtaaaac aagtactagg tgacggatca
     1321 gttgtctcta caggattgat cccatcagat atggtaaaaa gtccaacaac gaacgaagac
     1381 tttgcaaaag aagctggaaa attagtttct tatgacaaag ataaagcaaa agaatattgg
     1441 gaaaaagcga aaaaagaatt gggcgttgat tctttagaat ttgatttgat ggcttctgat
     1501 gatgattcat cgaaaaaagt aatcgaatat gttcaaaact caatccaaga aaacttggat
     1561 ggcgtaacag tcaaaccaac acctgttcca ttctctgtac gtcttgaccg ctcaacttcg
     1621 ggtgattttg acgcagtact aggcggatgg gctgctgact atgcagaccc aagcagcttt
     1681 actgatttgt ttgtcacagg taattcatac aaccgtggtc gctggagcaa tgcagaatat
     1741 gacgaagcag ttaaagcagc ttcatcaaca gacgctggcg atgaacaagc ccgttgggat
     1801 gatttccaaa aagcaatcaa gatcatctct gatgatatgg gtgtcgttcc ggtgtaccaa
     1861 aaagcagaag gtcacctagt gaatgaaaaa atcaaaggta tcgttcacca tgcggcaggc
     1921 gcttcatggg attacaaatg gacatacgtt gaagaataat cgttaatacc gtgagcatga
     1981 gaaaaaacga ggctgcggca aatgtcgcaa cctcgttctc atattcactt atcctaacaa
     2041 agaaaaaagc atgaaaatag cagaaagagc aattagtatt ccggcaatga tcagccaaaa
     2101 gctgtacata ccgtagctgg cagacgagag cgaggaaaat tctggttttt tctttcggtt
     2161 ccttgttcgt gcaagatgga ccgatcgctg aaaatgatcg aagaaacccg aagaaaagac
     2221 ccataaaaaa ccgccgataa tcagaaacgg caatgaccat aagaatagat cattagataa
     2281 ttgaatgagt gtaaaggaat ggttgatgag attttttatt aaaataacca gaatcagtag
     2341 aaaagctact gcgtagggga tagatttttt tttcatttga ttgctccttt atttttggtt
     2401 cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg gaaacattag
     2461 tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc acgctttggt
     2521 taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca tatacgaatc
     2581 aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt ttggataaac
     2641 cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat tttggaattt
     2701 ccttccaatt taaaaaccag ccagtcgcac atttgttagc tggaagaatc ggaccatcct
     2761 tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt ttaggaacga
     2821 tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta gcgatattag
     2881 gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt gcaatcaaat
     2941 tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta ccaacaattg
     3001 cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa atggtggaag
     3061 tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt tgggaaatcg
     3121 cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta ggaccgttag
     3181 ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt cctggtatcg
     3241 gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg gctgtaacga
     3301 tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg tacggtctag
     3361 ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg ataccaaagc
     3421 gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa aaaaataccg
     3481 aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat tcatggcgcc
     3541 gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc atcattttga
     3601 tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac gtggcttata
     3661 tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga aaagcaatgg
     3721 ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac gtaaatttct
     3781 ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg ggaatgagaa
     3841 tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt gtagcttacg
     3901 ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa
     3961 tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt tttgaaccag
     4021 gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg gcacgtatcg
     4081 ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga ttgacattag
     4141 gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct agtgtcatta
     4201 ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt ttaagcttta
     4261 tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc gaagggtaca
     4321 agacattcct gtatctgcct tatctattat ggattccggc agtaacttta tcggttatca
     4381 tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag
     4441 agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt ttgatacgta
     4501 tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag gagaaacatt
     4561 ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca tcatgcgctt
     4621 gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta acgatatcgt
     4681 tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca
     4741 agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg ctgagtccct
     4801 tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag aattgcttaa
     4861 attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc agttctcagg
     4921 aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg aaatcctgat
     4981 tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt tggagctgct
     5041 gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg accttggtgt
     5101 agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg
     5161 aacatcggaa gaaattttct acaacccgca acatccctat acttggggac tgcttggttc
     5221 aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt ctccaccgga
     5281 tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg cgatgaaaat
     5341 cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg cagcaacttg
     5401 gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac gtcgctgggc
     5461 tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg atgccaaata
     5521 aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac
     5581 atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt ttagttggag
     5641 aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat gaacctactg
     5701 atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa aaaggtttga
     5761 cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg ttgaatcctc
     5821 gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta gctaaatcat
     5881 ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg aatccaagcc
     5941 acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt ggtattgcac
     6001 gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct gcactagatg
     6061 tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa catcaattga
     6121 cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac cgaatcggtg
     6181 taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat aatcacggtg
     6241 ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca gaccatgaga
     6301 gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta cgtcaacttc
     6361 gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca tattacgcaa
     6421 aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga catagatgct
     6481 aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat tgattcgtac
     6541 ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga aaagttggca
     6601 acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag aatacccatt
     6661 cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg atcgggcttt
     6721 tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg
     6781 gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg gctacttaaa
     6841 gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg aaggatcgat
     6901 tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac tactttgtga
     6961 tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct atcatccgaa
     7021 gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc accagacaga
     7081 agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag
     7141 aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag taatggatct
     7201 ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg ggaaattggt
     7261 caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac atgctgattt
     7321 ttctattcac gactggcata aattaataac ataattgcgt tataagtccc aatggtataa
     7381 taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg taagaaagag
     7441 ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa tgaatgaaac
     7501 tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt tgtttctagg
     7561 aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca atagcagaaa
     7621 aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt tacgaataca
     7681 tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt ttttcttgaa
     7741 tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct tgagctgata
     7801 gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga aatattggag
     7861 catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta agaatttagc
     7921 aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta gaacttgcgg
     7981 ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt gactatggtc
     8041 aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg agatagccac
     8101 ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc agcgtacttc
     8161 cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt agctgactgt
     8221 gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt gctcaatatt
     8281 ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc tttctccagt
     8341 tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat aaggatgccc
     8401 ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc acatcagtac
     8461 gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt gataccagtt
     8521 aagaaattct tcttcacttg cttcatgttc ataatataat ttttcttctt ctttagaagc
     8581 aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct tattgtagca
     8641 gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt aaatatgaaa
     8701 aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca atactttata
     8761 ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa tgataaaacg
     8821 caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa aataaccata
     8881 catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag caatactaag
     8941 tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag atagatagtc
     9001 tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga tatgaaacaa
     9061 aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt gaacgtagat
     9121 agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg ctttttttat
     9181 ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag ccccgtggca
     9241 gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa acttcatatc
     9301 ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg gttgatgggc
     9361 tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga aacaaaagca
     9421 gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga tgtcaagaaa
     9481 ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga tgattaccat
     9541 aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat ttacttaggt
     9601 gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac acaacttgca
     9661 gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag tggacacgaa
     9721 gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc agaccgtcta
     9781 ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa aaacgaaatg
     9841 atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac aacttttact
     9901 tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat cgatgcatta
     9961 tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt cgaaaaatac
    10021 tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac gatctattgg
    10081 ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg gcatggctgg
    10141 ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg
    10201 cttgtggaac gttatggttt agatgcactc cgttattatt tgcttcgtgc tgttccgttt
    10261 gggtcagatg gtgtctttac gccagaagat tttgtttccc gtgtcaatta tgatctggca
    10321 aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata ttgtgatgga
    10381 ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc tacgacggct
    10441 gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa tacagcattg
    10501 gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac agaaccatgg
    10561 gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat ccatttagct
    10621 gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac gccagagaag
    10681 atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt acactttggc
    10741 gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc acgtttagat
    10801 atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca aacaaacgaa
    10861 gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga aaaacaaatc
    10921 aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa
    10981 gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag ccaagaccgt
    11041 caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat cgggaaaaaa
    11101 gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg
    11161 atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc aaaatccatg
    11221 ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt tgtgtcatac
    11281 cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta cttgaagcca
    11341 atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag aagtacgagc
    11401 ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac agaaaaaaag
    11461 cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt ttttattgtc
    11521 cttacattca taaagtttga atgcaatgac tttattacat ctatatttat atctaacagt
    11581 agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt gtgctcttat
    11641 atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg atgattgttt
    11701 tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt tttgtcttca
    11761 aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca taccagaaaa
    11821 tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt aacaagatca
    11881 accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg acttcactaa
    11941 gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc atccgccagt
    12001 tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag
    12061 gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc acaggacctt
    12121 tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt gataacgttg
    12181 caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt
    12241 ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga taagaacgct
    12301 taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa
    12361 aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa aaagaatggt
    12421 aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa gaaagaggat
    12481 gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag ctaccgttat
    12541 gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg ctagtactca
    12601 gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga tctgtgctgg
    12661 attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg taaaatatat
    12721 tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa gcttgattta
    12781 ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga cgaacttatt
    12841 acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt ctgtgacagg
    12901 gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt tgattgttaa
    12961 attaatacca attgctgtga ttgtcatatg gggattattg accccaggac aaggaactgt
    13021 ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc
    13081 gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa tggcagggga
    13141 aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga gttttgtaac
    13201 agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg atcatcttgc
    13261 cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa
    13321 gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt atacactaac
    13381 tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca gtaaacagct
    13441 gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc agttagcagt
    13501 agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt tgatttttgt
    13561 gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa aaaaagcgcc
    13621 ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta ttgcgattct
    13681 cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag cattgatcgg
    13741 tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga
    13801 agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct agatcttcgt
    13861 gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact tctgcaagta
    13921 gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt gattctcata
    13981 cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag cgagcacaag
    14041 aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt
    14101 tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat ccgacagagg
    14161 ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact ttaccaaagg
    14221 ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg aaagaagtcc
    14281 aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta ccaatcagta
    14341 ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa gatgttcgag
    14401 atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa cgatttcttg
    14461 acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc
    14521 aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat gccccttatc
    14581 tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt tatacagtag
    14641 aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa acatggacaa
    14701 atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca agagattata
    14761 gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc agatacgata
    14821 gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca tgcccaagag
    14881 actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat aagaaaaatc
    14941 attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca agctaaacca
    15001 aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt agaagcattg
    15061 aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac tagacaagac
    15121 cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga aaaactaggt
    15181 gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt gaagatgttt
    15241 cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca acatgagtga
    15301 atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac atggtttttc
    15361 atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa ggaaaatcgt
    15421 agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc
    15481 attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg
    15541 tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta ttgtacatca
    15601 agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg aggagcttcc
    15661 gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga tgcattttct
    15721 tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag tagctgatcg
    15781 tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg ttcaatatta
    15841 tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc agccaaatgt
    15901 agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag tgacagacga
    15961 aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa cgctttggaa
    16021 taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag caaaaagctt
    16081 ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac aagaattcgc
    16141 cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt
    16201 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatgt
    16261 ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga
    16321 ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa acagcttctc
    16381 atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact attatgccga
    16441 acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat
    16501 tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat gtgtcttgac
    16561 agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat attaggagag
    16621 agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat tgattgggat
    16681 gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt cctttacttt
    16741 ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg cagtggctgt
    16801 tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg cattgcgagc
    16861 attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc atccgagtat
    16921 cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt tcaattttgt
    16981 gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt actttattgg
    17041 taatatgcct gaaacatatt attctcaagg atatttttat acagtattca tcttgatggg
    17101 gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta tagcaggaac
    17161 actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa atcggaaaga
    17221 gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca aaaaaaaaac
    17281 tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc taaaaagagc
    17341 tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt ttgatgactt
    17401 gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg tcgactattt
    17461 cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta gaaccagttt
    17521 ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct gatttcacta
    17581 cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct ggacgaatac
    17641 cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt tttccttcag
    17701 gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat gtcacattaa
    17761 agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg ggtgtatcat
    17821 aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata acgattcggt
    17881 cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt gtttcaagac
    17941 gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca tctaaatttg
    18001 ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt cccaatgcca
    18061 cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat tctgtcagac
    18121 ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca tatttacgta
    18181 atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt
    18241 ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact tttccgccga
    18301 tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa gtagttgact
    18361 ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt gatagattaa
    18421 aatcagttac agaataattt tctgcattgt catatttttt atatacgttt ttcagagcca
    18481 tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt taaa
//
LOCUS       DAZKCY010000048        18306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000048 DAZKCY010000000
VERSION     DAZKCY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.048"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..2767
                     /locus_tag="ACG8XC_001511"
     CDS             44..2767
                     /locus_tag="ACG8XC_001511"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9191213.1"
                     /translation="MKTKILKELNDLLNSYDFPQYFPEECNAQYIAQKFQIKRNTASK
                     HLNELMEQKKVVKINGRPVLFFPVHTIERYISHSLDSFEFSSIDSFREKISKKKADHF
                     FDDLIGAKQSLKEAVQQGISAMKYPGGLPIMYFGSSGVGKSYLVEKLAEYCRQTGIIH
                     ENAPFIELNCAQYFNNPELLTSQLFGHMKGSFTGADSDHIGIIEAADGGIVFLDEIHR
                     LPPEGQEKLFIHMDKGVFRRVGDSGPWRLSHVRYIFATTEQEENFFLETFRRRIPIVC
                     TLPNYQDREISERKEIVFHLLEKESTLINKEILVSEAALSFLIRLDLYGNIGELDGLI
                     KQTIANKLFSGSNKKYIEIHISDLPSRYLLTENIELKNQIASQRFYIFSNGNYVHDCD
                     ETSTVTTNLRGLIHRVSKYYSECHDVNRNSEEFRRFVKDQMAEFNDRFIYQKNSEVLV
                     KYYLPEIQNIVQKISPSLFVHLSGNTVQAIATYLAIRDQWGYGKENASANEIEKLYEN
                     LKCNYLTDLNVFLLIFENALDTTLTIWDKLYISLCVISETGNEAKDINAIILAHGYAT
                     ASSIANVANRLVNQFVIDSIDMPLDISFSEVIDRLLHYIEYRKPKDGLVIFVDMGSLA
                     QIKTEIEQVIEVPTMIINNVTTEMAIETAQLIQSTSDIQKVVKKLPYSQFEKQVLYPI
                     KIRKRTIVVSCNTGLGTSIKIKEMMENNLSKDLGIEFLPYENETLRDTQQLEFLIKTK
                     DIIAIIGTDDPRVSNIPYISLEELFDGEIGKLYKVLLRVYGKEIAEAINQNIVKNSSL
                     NRLIDSLTILDAEKVVNQIEESLKDYQMRVGKTLSSLSKINLYVHISCMIERLIRNQG
                     VSSYPAIDQFLKRYETEVASIQKALSKIEKLYNIKIPMEEIAYVCNIIVSY"
     gene            2895..3317
                     /locus_tag="ACG8XC_001512"
     CDS             2895..3317
                     /locus_tag="ACG8XC_001512"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9191214.1"
                     /translation="MRQILCMSHGPLAKGMIETLSIIVGDLDNIDFRCAYVDGNNDVE
                     MIIDDYLKESMGNETIVVTDIFGGSINNEWLRRLPDLSKVYLISGMNLSLLVDLYLKF
                     QLVSSDQIEEIIDHAIQASSGSIKFCNQLDYEQTVDDF"
     gene            3335..3805
                     /locus_tag="ACG8XC_001513"
     CDS             3335..3805
                     /locus_tag="ACG8XC_001513"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9191215.1"
                     /translation="MIKLVRIDHRLIHGQVALTWTNAISADCILVANDEILNDPIQKT
                     TLKLAAPHNVKVVIKSIQDSIDAINSGKTDKYKLFVVTKTISDAKRLANGVEQIKSVN
                     IGSVKETSGSSALTHTIFVTEEERAELKGMVSEGIEVFAQQVPTDKAIDLKKSL"
     gene            3829..4611
                     /locus_tag="ACG8XC_001514"
     CDS             3829..4611
                     /locus_tag="ACG8XC_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9191216.1"
                     /translation="MDSLLVQAILLGLLTAFIVIDWMTGTNNLSRPIVSSVFVGLIMG
                     DLKMGIMMGATLELAFIGAITIGAARPPDTVSGGILGTAFAIATGKGAEFALTLAFPI
                     AALFLLVDNLLTLLVLPMIARKADRYAEKGELKNVSKMHVLGFILVKSLPRALFVGFS
                     FYLGSPVMERILNAIPTFVQDGINIAAGIMPALGFAILLQMLLKKDVFVYFVIGFALF
                     SYLHVPILGIAIFGACLAYIIVNMETKAAMGSRGEEMVEDDF"
     gene            4601..5425
                     /locus_tag="ACG8XC_001515"
     CDS             4601..5425
                     /locus_tag="ACG8XC_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9191217.1"
                     /translation="MTSSGKALTKDDLTKICIRSCALEASWNFERQQHMGFAFAMMPA
                     LEKIYSGNEEEKQKAAQRHLEFFNTAPPLSTFIMGIVASMEEENAKNPDDFDTSTITS
                     IKTALMGPLAGIGDSMFWGTLLVIGIGVGASLAKEGNILGPFLFLLIFNVPNFLLRYF
                     GVMQGYNFGTKLLENIESSGVMKTLTHAASILGLMVIGGMSGSMVSIKVAGSIGQGDE
                     ATKIQSIIDGILPNLLSVLCVFLIYWMLKKGFKTTYVLFGIFIFGIIAAFFGFLTT"
     gene            5460..6458
                     /locus_tag="ACG8XC_001516"
     CDS             5460..6458
                     /locus_tag="ACG8XC_001516"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9191218.1"
                     /translation="MLNYNAKEMKKKGKRIYGIREDVEKIAKQAYDSGISNIFFTASG
                     GSVAVMQPFEYVLMTESSIPVYSMTAAELVLMGHKQLNEKSLVILYSKSGDTKETIKA
                     AEYLKERNVPTVGIVSKEDTPLEALSTYAINYLDGRPQEMPLYFLIMSIMNLNGEFPE
                     YTRFADELKNLPTALNSVREQADEKGKKYAEEFKEDPYQIWIGSGNLWGITYSYSMCV
                     LEESQWLRTKSVSSPEFFHGTLELVEKGVCVTLLKTEGETRLLDARVEKFAVQYTDKF
                     NVFDTKDYELPGISDEFRKYFCAPVMWAALGRVSVHLEDIRNHSLDKRRYYRVVEY"
     gene            6475..7329
                     /locus_tag="ACG8XC_001517"
     CDS             6475..7329
                     /locus_tag="ACG8XC_001517"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFP9191219.1"
                     /translation="MTAYVIGFGDNVVDYYENLNQKFPGGNAVNFAVNAKKSGVKAYY
                     VGSLSSDPDGVLLQHAISSEGVDISFSEVIDEPTEQAHVTIKDGDREFIGGVRGSRLT
                     PALSAELIDLMKNSSLVHTSCHSRTETKIKELKKYDVKVSFDFSDLDKYRTHEYLSDV
                     CPSIYIAQFSVATDDQEEINRLISECKDFEVPYLLFTRGSKSPVFVDTRENKTYEGFI
                     RKVESPHDTMGAGDAYFAAFASNFVLSKREKSMIEHVIDCFHKGANLAYETLMIDGSF
                     GHATPINP"
     gene            complement(7903..9153)
                     /locus_tag="ACG8XC_001518"
                     /pseudo
     CDS             complement(7903..9153)
                     /locus_tag="ACG8XC_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            9670..9861
                     /locus_tag="ACG8XC_001519"
     CDS             9670..9861
                     /locus_tag="ACG8XC_001519"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191220.1"
                     /translation="MGKYSFEFKSTIVQEYLEGEGGLGYLAKIQGVRSKKGTRMDQSK
                     VRPKNLTFGGRYLYGKISF"
     gene            9842..11253
                     /locus_tag="ACG8XC_001520"
                     /pseudo
     CDS             9842..11253
                     /locus_tag="ACG8XC_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11328..12611
                     /locus_tag="ACG8XC_001521"
                     /pseudo
     CDS             11328..12611
                     /locus_tag="ACG8XC_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(12827..13639)
                     /locus_tag="ACG8XC_001522"
     CDS             complement(12827..13639)
                     /locus_tag="ACG8XC_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9191221.1"
                     /translation="MGDSKLTKKEINKVFWRSFALQGSESYTDMQGMGYSFSILPALN
                     KIYQGNDEKYYEAVYRNTEFFNTTPHISTFIMGMSLALEEENAKTDQFETETISSIKA
                     ALMGPVAGIGDSFYWGTFRVIAAGIGLSLAAQGNILGPILYFSIYTLLHFLGRYGCLR
                     IGYRMGANAIEKAFENNYLESFSLAASIVGLMSIGAMTSSLVRFTIPAVFKSKGGEIN
                     VQQMLDDIFPGILSLGLTFFVYFLLKKKVKVLWIILAIFVFSIVGVYIGLLG"
     gene            complement(13656..14387)
                     /locus_tag="ACG8XC_001523"
     CDS             complement(13656..14387)
                     /locus_tag="ACG8XC_001523"
                     /inference="COORDINATES: protein motif:HMM:NF015566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9191222.1"
                     /translation="MIECILIGIVGYLTTIDERYFGASMMNRPIIVGPVVGLILGDLH
                     QGILIGAALEAMFIGIVTIGAALPPDVGVAGTIATALAIKSGAGADVAVTLAMPFAVL
                     AQGLNMLAFTINSFTVSQGRKAIENRKLSSFQKWHFFPLIVRLPSAVLTFLVVYFGAN
                     ATESFVNIMPEKLMTGFTVASGLLPAVGFAMLIQGMIDKKSVPYFFIGYLLAAYLGVP
                     VVGVAVAAIMVALLVIQTKRNPVNI"
     gene            complement(14404..14859)
                     /locus_tag="ACG8XC_001524"
     CDS             complement(14404..14859)
                     /locus_tag="ACG8XC_001524"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9191223.1"
                     /translation="MIKLLRIDDRLIHGQVAMAWTKSVSADYIIVVDDASANDKMKQM
                     ILSLAKPVSTGLDIITSADFLSTFEKRKNQNLMIVTSSPKSAFEIVNAIPGEVDSINI
                     GGLRYAEGKEKVNEYVAITSEDKNYLAKLKESGFKLIMQATPTGKAKIY"
     gene            complement(14852..15262)
                     /locus_tag="ACG8XC_001525"
     CDS             complement(14852..15262)
                     /locus_tag="ACG8XC_001525"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9191224.1"
                     /translation="MYTIILASHKNLAKGMKETVEYILGPAPFIKVMTAYTDDNYDIE
                     KDTNILLDGTDKVLVITDLLGGSVNNHWMNYVYEKKLTKKITVIAGMTLSLIMELSMN
                     IEDYKLREKISMIIAESQKSIINCSELMEVEDND"
     gene            complement(15793..16752)
                     /locus_tag="ACG8XC_001526"
                     /pseudo
     CDS             complement(15793..16752)
                     /locus_tag="ACG8XC_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(17613..17828)
                     /locus_tag="ACG8XC_001527"
     CDS             complement(17613..17828)
                     /locus_tag="ACG8XC_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFP9191225.1"
                     /translation="MKKFIIPCIFLFLSILLFSTSILVGSYLDSSGMLIEPAFFCVPL
                     GYLTLGLAFFTALYSFVKQRLLKKYSA"
BASE COUNT         6045 a         2955 c         3537 g         5769 t
ORIGIN      
        1 atagtgaaaa tcatttatta ttaagtaaag gaggtgggtt catttgaaaa cgaaaatttt
       61 aaaagaatta aatgatctac ttaacagcta tgattttcca caatattttc cagaagaatg
      121 taacgcgcaa tatattgccc aaaaatttca aataaaaaga aatacggcta gtaagcatct
      181 aaacgaacta atggaacaga aaaaagttgt gaaaatcaat ggtcgtcctg ttcttttttt
      241 tccagtccat acaattgaac ggtatatatc acattcatta gatagttttg agttttcttc
      301 aattgattcg tttagagaaa aaatttcaaa gaaaaaagca gatcattttt ttgatgattt
      361 aattggtgca aaacaaagtt taaaagaagc ggttcaacaa gggatatcag caatgaaata
      421 tccaggtgga ttaccaataa tgtattttgg atcgtccgga gttgggaaga gttacttagt
      481 ggaaaaacta gctgagtatt gccgacaaac tggtattatt catgagaatg ctccgttcat
      541 cgagttaaac tgcgctcagt attttaataa tccagagtta ttaacaagtc agttgtttgg
      601 ccatatgaag ggatcgttta ccggcgccga ttctgaccat attggtataa tagaagcagc
      661 agacggtgga atcgtctttc ttgacgaaat tcatcgttta ccaccagaag gacaagagaa
      721 actttttatt catatggata aaggcgtttt tcgaagagtt ggagatagtg gtccttggcg
      781 tttatcacat gttagatata tttttgcgac aacggaacag gaagaaaatt tttttctaga
      841 aacttttcga cgaagaattc cgattgtttg tactttgcct aattaccaag atagagaaat
      901 tagcgaacga aaagagattg tcttccattt attagagaaa gagtctactc tgatcaataa
      961 agaaatatta gtttctgaag ccgcccttag tttcctgatt cgcttagatt tgtatggaaa
     1021 cattggtgag ctggatggac taataaaaca aactattgcg aataaactat ttagtggaag
     1081 caataaaaaa tatatcgaaa ttcatatttc tgatttacct agcaggtact tgctaacaga
     1141 aaatattgag ttaaaaaacc aaattgcgag ccaacggttc tatatcttta gtaatggtaa
     1201 ctatgttcat gattgtgacg agacttcaac tgtaactaca aatttacgag gtttgattca
     1261 tcgtgtttct aaatattatt cagagtgtca tgatgtgaat agaaatagtg aagagtttcg
     1321 acgttttgtg aaggaccaaa tggcagaatt taatgatcgt tttatttacc aaaaaaattc
     1381 agaagtctta gttaagtatt atttaccgga aatccaaaat attgttcaaa aaatctctcc
     1441 cagtcttttt gttcaccttt ctggcaatac ggtccaagct atagctacct atctcgctat
     1501 tagggatcaa tgggggtatg ggaaagagaa tgcttcagcg aatgaaattg aaaagttata
     1561 tgaaaaccta aagtgtaact atttgacgga tttaaatgta ttcttactga tcttcgagaa
     1621 cgccctggat acaacattga ctatttggga taaactgtat atcagtttgt gtgttattag
     1681 tgagaccgga aatgaagcga aagacattaa cgcaattatt ttagcccatg gatatgcgac
     1741 agctagtagt attgctaatg ttgccaatcg tttggttaat cagtttgtca tagattctat
     1801 tgatatgcct ctagatatca gctttagtga agtaattgac agattacttc actatataga
     1861 gtatcgaaag cctaaagatg gactagtgat tttcgttgat atgggaagtt tagctcaaat
     1921 taagactgaa attgaacaag taatcgaagt tccaacaatg attattaata atgttacaac
     1981 cgaaatggct attgaaaccg cacaactgat tcaaagtacg agtgatatcc aaaaggttgt
     2041 aaaaaaattg ccgtattctc agtttgaaaa gcaagttcta tatcccatca aaattagaaa
     2101 acgtacaatc gttgtttcct gcaatactgg tttgggtacc tccataaaaa ttaaagaaat
     2161 gatggaaaat aatttgtcga aagacttggg cattgaattt ctgccatacg aaaatgaaac
     2221 tcttagagat actcagcaat tagagtttct cattaaaaca aaggatatca ttgccattat
     2281 cggaacagat gatccgcgtg tgtcgaacat tccctatatt tctcttgaag aattatttga
     2341 tggtgaaatt ggcaaactct ataaagttct tttgagagtc tatggaaaag agattgctga
     2401 ggcaattaat caaaatattg ttaaaaatag ctcattaaac cgcttgattg acagtcttac
     2461 tattttagat gccgaaaaag ttgtcaatca aattgaggaa agtttaaaag attatcaaat
     2521 gagggtgggg aaaacgttat catcattgtc caaaattaat ctttatgttc atatcagctg
     2581 tatgattgag cgactaattc gtaatcaggg agtaagtagt tatccagcaa tagatcagtt
     2641 tcttaagcga tacgaaactg aggttgcttc tattcaaaag gcacttagta agattgagaa
     2701 actttataat attaagattc ctatggaaga gattgcatat gtgtgcaaca tcattgtaag
     2761 ttattgataa aataaagtga cactattcac agcgatggaa atagtgtcac tttatttttt
     2821 gtacgccctt tcatttaagt ggtgacagta attggcacag tatttgctaa taacaaagcg
     2881 aaaggagttc gggtatgaga caaatattat gtatgagcca cggtcctcta gcaaaaggaa
     2941 tgattgaaac tttgagtatt atcgtaggag atttggataa tattgatttt cgatgtgcct
     3001 atgtcgatgg aaataatgac gtagaaatga tcatcgatga ttatctaaag gaatctatgg
     3061 gaaatgagac gattgttgta accgacattt ttggtggaag cattaataat gagtggctaa
     3121 gacggttgcc agatctttct aaagtctatt tgatttctgg aatgaattta agtcttttag
     3181 tggatttata tttaaaattc caattagttt catctgatca gattgaagaa attattgatc
     3241 atgcaattca agcatcttct ggttcgatta agttttgtaa tcagctagat tatgagcaaa
     3301 cggttgatga cttttaaata aaggagggaa gagaatgatt aaattagtga ggatcgatca
     3361 tcgtttgata cacggtcaag ttgctttaac ttggacgaac gcaatcagtg cggattgcat
     3421 tttagtagcg aacgatgaaa ttttgaatga tccaattcaa aagacaactt tgaaattagc
     3481 cgcaccacat aacgtgaagg ttgttatcaa atcaattcaa gattcgattg atgcaattaa
     3541 tagtgggaag acagataaat acaaactatt tgtagttacc aaaaccattt ccgatgctaa
     3601 acggttggca aatggggttg aacaaattaa atctgtaaat atcggtagtg ttaaggaaac
     3661 tagtggcagt agcgctttga cgcatacaat ttttgtaaca gaagaggaac gagcagaact
     3721 taaaggaatg gtctcagagg gcattgaagt gttcgctcaa caagttccaa cagataaggc
     3781 aatagattta aaaaaatctt tgtaataaaa aaataagggg gtaatttcat ggatagtctc
     3841 ttagtacaag caattttgct tggtctcttg actgcgttca tcgtaattga ctggatgaca
     3901 ggtacgaata acttatcaag accaatcgta tcttccgtct ttgttggttt aattatgggc
     3961 gatcttaaga tggggattat gatgggggca acactggagt tagcatttat cggagcaata
     4021 acaatcggag cggcacgtcc gcctgatacg gtatctggtg ggattttagg gactgcgttt
     4081 gctattgcaa ctggcaaggg agcagaattt gcgttgacct tggcattccc aatcgctgcg
     4141 ctattcttat tagttgataa tctactaaca ttgcttgttc taccaatgat tgctagaaag
     4201 gcagatagat atgctgaaaa gggcgaacta aaaaatgtgt cgaaaatgca tgttttaggt
     4261 tttatcttag taaaaagttt accacgggct ttatttgtcg gattctcatt ttacttagga
     4321 agtcctgtta tggaacgaat cttaaatgct attccaactt ttgtccaaga tgggattaac
     4381 attgctgcag gcattatgcc agcactaggt tttgctattt tattgcaaat gcttcttaag
     4441 aaagatgtct ttgtatattt cgtaattggt tttgcactct ttagctactt acatgtgccg
     4501 attctgggga ttgcaatctt cggcgcatgt ctagcttata ttatagtaaa catggaaacc
     4561 aaagctgcaa tgggttcaag aggggaggag atggttgaag atgacttcta gtgggaaagc
     4621 tttaactaaa gatgatttaa caaaaatttg tattcggtca tgtgcattag aagcatcatg
     4681 gaattttgag cgccaacaac atatgggctt tgcttttgct atgatgccag cgttagaaaa
     4741 aatttatagt ggcaatgaag aagaaaagca aaaagcagct caaagacatc tagagttttt
     4801 taacacagct ccgccattat caacattcat aatgggaatt gtggcttcta tggaagaaga
     4861 aaatgccaaa aatcctgatg actttgatac ttctacaatc acttctatta aaactgcatt
     4921 gatgggacca ttagctggta ttggtgattc aatgttttgg ggtaccttat tagttatcgg
     4981 tatcggtgta ggtgcttcat tagcaaaaga aggcaatatt ttgggtccat ttttattttt
     5041 actaatcttc aatgtcccta attttcttct ccgttacttt ggagttatgc agggatataa
     5101 ctttggtaca aagctattag agaacattga atcctctggc gtcatgaaaa cgttgactca
     5161 tgctgcctca attttgggtc ttatggtaat tgggggcatg tctggaagta tggtatcaat
     5221 caaagtagct ggttctattg gacaaggtga tgaagcaaca aaaattcaaa gtatcattga
     5281 tggtatttta ccaaatctgc tgtcagtatt atgtgtattt ctgatttact ggatgcttaa
     5341 aaaaggcttt aaaacaactt acgtgttatt cggaatcttt atctttggta tcatagcagc
     5401 cttcttcgga tttttgacaa cttaattgaa taatctaaat aattgtaagg agagaactaa
     5461 tgttaaacta taatgcaaaa gaaatgaaaa aaaaaggcaa acggatttac ggtattcgtg
     5521 aagatgtaga aaaaattgca aagcaagctt atgattctgg aattagtaat attttcttta
     5581 ctgcttctgg tggatcggta gccgtaatgc aaccatttga gtatgtattg atgactgaat
     5641 caagtattcc tgtttactct atgactgcag ctgagcttgt cttaatggga cataaacagt
     5701 tgaatgaaaa atctttagta atcttatact caaaatctgg agatacgaaa gaaactatta
     5761 aagcagctga atatttaaaa gaacgaaacg tgccaaccgt tggtattgta agtaaagaag
     5821 atacaccgtt agaggctcta tcaacttatg caatcaatta cctagacggc cgccctcaag
     5881 aaatgccact ttatttctta attatgtcta tcatgaattt gaatggggaa tttcctgaat
     5941 acactcgttt tgctgatgaa ctgaaaaact taccaacggc tctgaattca gttagagaac
     6001 aggctgatga aaaagggaag aagtatgccg aagaatttaa agaagatcct tatcaaattt
     6061 ggattggtag cgggaattta tggggaatca cctactctta ttcaatgtgt gtgcttgagg
     6121 aatctcaatg gttgcgtaca aagtctgtct catcaccaga gtttttccat ggtaccctcg
     6181 agttagtcga aaaaggagtt tgtgtaacac ttttgaaaac tgagggggaa acacggcttt
     6241 tagatgctcg agttgaaaaa tttgcggtcc aatatacaga taaatttaat gtttttgata
     6301 ctaaggatta tgagctacca ggtattagtg acgaatttcg aaagtacttt tgtgctccag
     6361 ttatgtgggc agccctaggt cgcgtcagtg ttcatcttga agatattcgg aaccactctt
     6421 tggataaacg tcgttattac cgagtagttg aatactaaaa gtaggtgatt aattatgaca
     6481 gcgtatgtga ttggatttgg cgataatgtt gttgattact atgagaattt gaatcaaaaa
     6541 tttccagggg gaaatgcagt taattttgct gtgaatgcaa aaaagagtgg tgttaaagca
     6601 tactatgtcg gtagtctatc atctgatcct gatggcgtgt tattacaaca cgccatcagt
     6661 tctgaaggtg ttgatatctc tttctcggaa gttattgacg agcctactga gcaagcacat
     6721 gtaactatta aagatggaga tagggagttt ataggtggcg tgcgtggctc ccgattgaca
     6781 cctgctctct cagcagaact tattgatttg atgaagaaca gttcattagt tcatactagt
     6841 tgccatagtc gaacagaaac aaaaattaaa gaattaaaaa aatatgatgt taaagtttct
     6901 tttgattttt ctgacttaga taaatataga acgcatgaat acttgtcaga tgtttgtccg
     6961 tcaatttata ttgcacagtt ttcagtcgct acagatgatc aagaggaaat taatcgctta
     7021 attagtgaat gcaaggactt tgaggttccg tatcttcttt ttacgcgtgg tagtaagagc
     7081 cctgtttttg ttgacactcg agagaataag acttatgaag gattcattcg caaagttgag
     7141 tcacctcatg atacaatggg cgcaggtgat gcatactttg cagcttttgc aagtaatttt
     7201 gtcttatcta aacgtgaaaa atcaatgata gaacatgtta ttgattgttt tcacaaaggt
     7261 gcaaaccttg catatgaaac attgatgatt gatggctcct ttggtcatgc tacacctatt
     7321 aacccttagc tttgtgcttt gggcttttat tgctggaatt tgtatagtag actcatcata
     7381 actatatttt tgtatgtgaa aaggcaagag gctatgatga aaataacact ggctaattgc
     7441 atgagggagc atatcgttgt taaagcgtca ttgatagtat gaataaatat ctcgataata
     7501 ttatattgtt atttacttaa tttattgatt agtcaagcaa agataatctt ctgttactta
     7561 acgagactag taggtacaag cacttcatgt tatgtacgac ccaaaaatat tgctgttgat
     7621 tatttgctga aattattgcg agcttcaatt tatttttaaa ggacttggta ggctgcgtga
     7681 tgaaataaga cttggaaggc tgtctaaacc aatatatagt agactaaata gtaaaaaaga
     7741 gcatggtaat tgtctctttg tcaataagtc taatggtaat aagacaagtt ggagattgag
     7801 aatattttta ttgtgtaacc tattttgaag cgtacttaac tagccccggc caaaatcaaa
     7861 ttttttcggg gtatatcaag ggtttgcgtc aataacagaa tgttatttat ctatcacgtt
     7921 ccttggtggc gtgtagagtt gatggttacg tagtagcgta tctaccagac gcgtaaattt
     7981 tcttgcggtt aagacgtgtg ctcgtttgtg tttatttttg ggacctcttt acatttgctt
     8041 tgataaaaag ctttatattg aggaagatat ttttttacag agttggaagc ttcaactaag
     8101 tagtatctaa aatagcgact cctttgtttt gtcagaggtg tattttgtga ccgatagtta
     8161 acagattgtt tgcctttcca acataagcct gcgtattttg ccaattttgt ttgatcttca
     8221 aatcgttcaa tttgaccgat ttcagcgatt aatccagcgg cgtgtacctt tccaatacct
     8281 ggaacactag tcaaatattg atattctgga ataacgacaa caaattgctc ggttgcttta
     8341 tccaagtctt tgatgttctt ttcaagcgca cgaatttcac atacaaaaac atttagaacg
     8401 acattgatgg attcttatgc aagactatct aaccgataac tcacggcaat tgcacgttga
     8461 atcgatttgg catttttttc tggatcttta aagcgacatt tcctttttct tttagtaagt
     8521 cacagaaagc ttccaaagga agcacagcca attcatctag cgtgaagtct tctgtcacaa
     8581 gagtaatgac agtggcacta tagagacttg tcgattcatc gcgcatttat cgagtaagcg
     8641 tattgcattt gtacgttaga ttttccagaa agtgttgttt tgtacgagtg aagttgctta
     8701 atgagtcgat agcgagtgcg tgttaaacgc tgtaaagcca cgtacttctc ttctttgata
     8761 ggagaagttg tgaaccgttg aatacgcaat aaatcagcaa ttcttaacgc gttgtttcga
     8821 tcagtcgtat cttcctcaaa catgcgactg aattgtttca tccgaaaagg attttcaatc
     8881 ctcacaagtg cattgagttt ttttagctct gcatcttcat ggaaaaacat cgaaggatga
     8941 aagctgtaca tggacgttta attccatctc aataacgatt tgaccgaagt gattgttttc
     9001 attgaacaca agaatcagtt ctcgtgtcaa tggagccctt tcaatgtcat tagcaacaga
     9061 attttcagtc aatatagata gctgatcgtc gtctgttagg aagcaaacgt ctaatttttc
     9121 agaactaacg tcattaccaa caaataattt catggaataa aaacctattt tcgatataaa
     9181 ttctagaaaa tgcttcgata ctgtgggatt ccacagaaac tcactgtgtg gtcacgacct
     9241 cgcctaatag aatacggtaa taaaagagct aatgagttga tagtaaacac agcgctttgg
     9301 ataacagact tatgaaggta gacaaagcta caggactgat tagcaaattc tcgttgaatc
     9361 ctttccatac atccattgta ctctgagaaa tcacacaata acgaaataaa ttccggaagg
     9421 aatatgttat tcaaaaaaat ttgtgtttta tagatacttt aaaagaaact tattaggcaa
     9481 aatatatttt acgaagactg atgagttgtg caaaatcaaa tctgagtcag aatgaacttc
     9541 attctagctc agatttttgt ttaatgactt cagtcagttt cttgcattac gtgaatgaaa
     9601 atgggactgg tgatgtccaa accctaaaaa gttagaccaa aatctaactt caggggggat
     9661 ggtattttta tgggaaaata tagctttgaa ttcaagtcaa caatcgttca agagtatctt
     9721 gaaggagaag gtggattagg atatttagca aaaatacaag gagtgagatc aaaaaaaggt
     9781 acacggatgg atcaatcaaa agttagacca aaaaatctaa cttttggggg tcgttatttg
     9841 tatggcaaaa tatccttttg aattaaaact aaaaatcgtt aaagaatatc ttagtggcgt
     9901 gggcgaatat cggcatcttg ccaacaaata cggtatttca agcaagtctc aagtaagtga
     9961 ttgggtgaat gcctatcgtg aatacggaga ggaaggtcta ttgaaaaagc atcaaaatca
    10021 gaaatattct gttcaattta agttaaatgc gctagagtta tatcaaacaa gtgagatgtc
    10081 ctatcgagaa gttgccagca ctttagaaat gaacaaccct gcgcttattg ccaattggat
    10141 gcgtaatttc agagaagcag gtattgaggg gctctcaaaa gagaaagggc gaccacttac
    10201 tttgactaga aaaaaagaaa acaaaaaaga aaatacaaca actgaagaac gcgagcgtat
    10261 caaggcatta gaaaagcaag tccgatccct tcaaattgag aatgcctttt taaaagagtt
    10321 gaggaagctg cgaaaacagg aagcccagca aagacgcaag aatcaatcgc acgaatcatc
    10381 gccagcctcc gaggatcatt caaactagtt gagcttcttg aaacattgaa atttcctaaa
    10441 gcaacctata tgtattggca gaaacggttt gacagaccaa acccagacca agaaattgaa
    10501 gaaaaaatgc ttgaaattcg caaggagcat aaagactatg gctgtctgcg actaaaaaaa
    10561 gaattagaga atcaagggat tcatgtcaat aaaaagaaga ttcaacgtct gataaaaaaa
    10621 ctaggtatcg aagtaaagtc ttacactcga aaatctcgac ggtacaattc ttatcatggg
    10681 aaggttggca ctgtcgccaa aaatcgtatt catcgtcggt tttatacaaa tatctgtcac
    10741 caaaaaatta caactgatac cacagagttt aagtattatg aagtagacac cacaggtgtc
    10801 gttcgtcaga aaaagctgta tttagatcct ttcatggatt tctacaatag tgaaattcta
    10861 tcttatcgga tttctgaaaa accaaacgca ctagccataa tggaaggatt agaagaagca
    10921 atccagatga caaatgattg tccatttcgt agaaccttcc attcagatca aggatgggcc
    10981 tatcaaatga atgcttataa gaataaattg aagcagcata aaatcttcca gagtatgtct
    11041 cgtaaaggga attgtttaga taactcacct atggaaaact tctttggttt attaaagcaa
    11101 gagatttttc atggagaagt ctatcggagt ttagatgaat tgaaaacaaa aattgaccaa
    11161 tatatctatt attacaacca taaacgcatc aaaaagaaat taaattggcg aagtccggtt
    11221 cagtttcgag aagctgtaaa aacagctgtc taacagttaa ttttatcgta caaaaaagga
    11281 gtggatttct ccactccata aaagtctaac tttttggggt cgctacaaat gcttatcgag
    11341 aatttggaga agaaggcctc ttgcgtaagc gaaataagcg aagttattct gttcaattca
    11401 agatagatgc gatagagtta taaaaaacgg gtgagctatc ctatcgtgaa gtggcgaatc
    11461 atttagggat aaaccaacct gcgttagttg tcaattggat gaaaattttt gttcaaaagg
    11521 attgcacggg ctctcaaaaa tgaaagggtc ctcctccatt ttgtctaaga aagaataaaa
    11581 ggaagaaaca agcaaactga cgcctaaaga gcataatcgt atcaaagaat tggaaaaata
    11641 agtgcgtttc cttcagattg agaatacctt tttaaaagaa ttgaggaagc ttagaaaaca
    11701 ggaagtctaa caaagacgaa cgaatcaatc gcacgaatca tcgacaacct ccgaggatca
    11761 ttcaaattag ttgagcttct tgaaaccttg aaatttcctg aattaacctt tatgtattgg
    11821 caaagacgat ttgatcgaag aaattcagat caagaaatcg aagaggaaat gcttaaaata
    11881 agaagtcaat ataaagatta tggctattta cgtatgacaa aggaattgtg ggaccgtggg
    11941 ttccatgtta acaagaaaaa agtacaacgt ttgattcgaa agctgagtat tgaagtacga
    12001 tcatttacga gaaaatatcg ccgctataat acttatcgtg gaagaatcgg catgattaca
    12061 aaaaatcgaa ttcatcgacg cttttacaca agcaactgtc atcaaaaggt tacaacagat
    12121 acgacagaac taaaatattt tgaaatggat aaatacggtg ccaatcgtga aaagaagctg
    12181 tatcttgatc catttttaga tatgtacaac agtgaaattt tattttatcg catttctgaa
    12241 aagccaagtg cgttggctat catggaagct ttagaggaag ctatctgcgc aacggaatgt
    12301 atctatcgtc gaacttttca ctccaaaaag gatggaccta ccaaatgaaa gcttatagtc
    12361 ataagttgaa agaacataag atcttccaaa gtatgtcccg taaggagacc tgcttagata
    12421 attcgatcat tgagaacttc cttagtctat taaagcagga aatttattat ggaagagact
    12481 atcgtatctt tgaagaattg aagcaagcca ttgatggcta tattcattac tacaaccatg
    12541 agcgtatgaa aaggaaacta aactggcaga gtctggtgca atttagagta atagcattca
    12601 aagctgtcta aaatcatcaa gaaaagcaaa gaaaaacgga gtgaattttc tccactccga
    12661 aaacatctat cttttttagg tcactacaga atcaattctg attcatcaga cccataaatt
    12721 atagatccta ataatttata ccatgctctt tttactcata tttacattta tctttgggta
    12781 ttttatggtt taagttgatg tctgatctag ttttattctg gattaattaa cctaataatc
    12841 caatataaac tccaacaata ctaaagacga agatagcaag tataatccaa agaaccttta
    12901 cttttttctt taatagaaag taaacaaaga atgttagccc caatgagaga attcctggaa
    12961 aaatatcatc gagcatttgc tgaacattta tttctccacc ttttgattta aatacggcgg
    13021 gaatagtaaa gcgtaccaga gatgaggtca tggctccaat tgacattaaa cctacgatag
    13081 atgctgctaa cgagaaactt tcaaggtaat tgttctcaaa tgctttttca atggcgtttg
    13141 ctcccatccg atatccaata cgtaagcagc catatctgcc aagaaaatgt aacagcgtat
    13201 aaattgaaaa atacaatatt ggacctaaaa tgtttccttg ggcagctaaa gataagccga
    13261 tgccagcagc aataacgcga aatgttcccc agtaaaaact atcaccaata ccagctacag
    13321 gccccatcaa tgcagctttt atcgatgaaa ttgtttcagt ttcaaattga tctgtttttg
    13381 cattttcttc ttcaagtgct aatgacatac ccataataaa ggttgaaata tgaggcgttg
    13441 tattaaaaaa ttccgtattt cggtaaacag cttcataata tttttcatca ttcccttgat
    13501 atattttgtt taaagcaggt aatatagaaa aactatatcc cattccttgc atatcagtgt
    13561 atgactcgga tccctgtaaa gcaaatgaac gccagaaaac cttgtttatt tcttttttag
    13621 ttaatttaga atctcccata attatcactc tccctttaaa tatttacagg attgcgttta
    13681 gtttggataa ctaataaagc aaccataatt gcagcaacag ctacaccaac tacggggact
    13741 cctaaatatg ccgctaaaag gtatccaatg aaaaaataag gaactgattt tttgtcaatc
    13801 attccttgaa ttagcatggc aaatccaaca gcggggagta aaccagaagc tacggtgaaa
    13861 ccggtcatta atttttctgg cataatattt acaaaacttt ctgtagcatt ggcaccaaaa
    13921 tatactacta aaaatgtaag tactgcggaa ggtaagcgaa cgattaatgg aaaaaaatgc
    13981 cacttctgaa aagatgataa tttgcggttt tcaattgctt ttctaccttg tgaaacggta
    14041 aaggagttaa tcgtaaaagc caacatgttt aatccttgtg ccaatacagc gaaaggcatc
    14101 gccaatgtaa cagctacatc agcacctgct ccacttttta ttgctagagc cgttgcaata
    14161 gtaccagcta cacctacatc tggtggtagt gcagcaccaa tcgttacaat gccaataaac
    14221 attgcttcta atgctgctcc tatcaagatg ccttgatgga gatcccctaa gataagtcca
    14281 acaacaggtc caactattat cggtcgattc atcatagatg ccccaaaata tctttcatct
    14341 attgtcgtta aatacccaac tatgccaatt aaaatgcatt caatcattct tattccccac
    14401 cttctaataa atcttcgctt ttccagttgg agtagcctgc ataattaatt tgaagccgga
    14461 ctcctttaat tttgctaagt agtttttgtc ttctgatgtg atagccacat actcgttaac
    14521 tttctctttt ccctcggcgt accgtaaacc accgatatta attgaatcga cttcccctgg
    14581 gatagcgttt acaatttcaa atgcgctttt aggggaacta gtgactatca tcaaattttg
    14641 atttttcctt ttttcaaagg tacttaaaaa atctgctgaa gtaataatat ctagtcccgt
    14701 tgatacaggt tttgctaatg ataaaatcat ttgtttcatt ttgtcattag cagaggcgtc
    14761 atcaactaca ataatgtagt cagcagatac ggacttagtc caagccattg caacttgacc
    14821 gtggatcagg cgatcgtcaa ttcttaatag tttaatcatt atcttctacc tccattaatt
    14881 ctgaacagtt tataatgctc ttttggcttt cagcaataat catggaaatt ttttctctca
    14941 atttataatc ttcaatgttc atacttaatt ccataattaa ggacaaagtc atacctgcaa
    15001 ttactgtaat tttttttgtt aatttttttt cgtaaacata attcatccaa tgattattta
    15061 cgctaccacc aagtaaatca gtgatgacaa ggactttatc agttccatcg agaagaatat
    15121 tcgtatcttt ttcaatatca tagttatcgt ctgtgtatgc agtcattact tttatgaatg
    15181 gtgcagggcc aaggatatat tcgactgtct ctttcattcc tttcgctaaa tttttgtggc
    15241 ttgcaagaat aattgtgtac ataatccacc tccatttatt tataaataat ataccattgg
    15301 taatctaaat gtcaataaat tatttcgtat taagaataaa taaaatacct ttggtaatta
    15361 gtggggcgaa tctaaaaact ttaactaaaa ttctcttttg taaatttttt tgaacgcggc
    15421 tatcgtggtg attttttatt tctgtttgtt taatgaattt gtaaattagg aggtgatttt
    15481 tgtgatggtg taaacaaagc ctataaaaat gaagtaaagc attgtgtttt gtccagatgt
    15541 caaatctttt atgtatcggt tgtatgtata gtatgttaca cccaaaatag ataacccaag
    15601 tccaaatgga gagacagatg ttctttatat tactggcgaa gacatgaagt attcttataa
    15661 aatatctaaa aatgagtata agtttaaaga aaataattta tctatataaa ctattctgct
    15721 ttgattagca agtctttcaa tcggtaaaat attatttgaa tagatgggat atggtttatt
    15781 attttctaaa tgtcaaatta agttagacaa ttgctcctca cttatgtaac tcaaaaatac
    15841 ttctaacgat gttcgataat ttaaagattt ccttggtata tgatttcttt tggaggcaac
    15901 cgccgccaca aatttttggt caacctgatt gaaatccatt gtattaggaa gtccgtcttt
    15961 acgtaacaat ccattagaat gttcatttaa tccccgttgt gacagcgttc caggatctgc
    16021 aaaataaata gaaatatcgt tagcattact catgtttttc caattcgaaa attcttttcc
    16081 gcaatcaaag gtaatggatt tgaacaagtt tcggggaagc ttttgtagcc agtcagttgt
    16141 cactttttca atatctagtg ctttccgtcc ttcgggtttt aaagtaataa tcacttttga
    16201 caagcgctca accagcgtga tgacagcact tttatgatgg acacccacaa tagtgtcgcc
    16261 ttccaaatgt ccaaattcct gttgaaattc aggatagtcc gtgagacgtt ctgaaatatt
    16321 ccgtttgaag gcttgtttcc ctcgacgttc tttatgcccg ttgggtttcc gttttccttt
    16381 catgggtagt ttcgagcaat caaattagtt tgttttaaac ttgcgataaa gggttcgaac
    16441 agaacatgaa attgaaaact cagcacgacc gataatcaca tcgggtgtcc atccttgcgc
    16501 caccttgttt cggatatacg catcttgttc tgctggcaaa caaatgggtc gtcggccgca
    16561 ccgtgtttta tttttttgat attgttggta atagtcatgg atagattggc cttgtttcag
    16621 gaagttgtac acattgtaga tggtctgtct ggaacgattt gaggtcttag cgactacggt
    16681 aacagattga tgcatattga aataggcttc tatcatcacg agttcatttg ttgtaagatg
    16741 ggtataggtc atttgtcttc actccttata attctttggt cggaactatt ttgagtgtag
    16801 cacaaatgac ttttttagtt gtctagctta attttacaat ctgcgtttaa taaactctca
    16861 gtgggatagt ttgactccgt atatgactcc ggttgatttg attgaattca gatgattata
    16921 catactataa atttctctat tatgttgcca tttgatttga ataatgctga aagaaggatt
    16981 ttagtctata acgaacaagg gaataaaacc aataaaaaaa gatatagaat atcaatataa
    17041 caaaaagaga ttgtgacata attctctgaa aaaagctcta gaaatgacga gtcattcgtc
    17101 atttctggag ctttttaggt aaaataaaag agtacgagag gatactcgca ctctaaatca
    17161 ttcaaccctt gaaaggatca aaatcatgca tacaaattat aacatgaatc aactcacttt
    17221 agatatcaca acttcttatt ccaaaagaag atagcaccgc tgggtttgtc aatgaactcg
    17281 ttgaaaccct tgaaatcaac gagccgtatc tttttggccg ccctaggaat tgtgatttga
    17341 gtgccatgtt aaaattggtt cttttcgcat attgaaaatg attgaagagg actatcggta
    17401 ttacaaaaga caatataacc agcaggtgaa aaaatggaga aggtaattcc gaaaaaagct
    17461 tcttttggcg attgggaata attgttggta atggaggaat aaaatgaatg tttatgcaga
    17521 cgtttgatgt ctacatcaac atccattctt aatactcgaa ggttcgttgt tgaaaaaact
    17581 aacttttctt caacagagga tttacgtgca aatcaagccg agtatttctt caataatctt
    17641 tgtttgacga aactgtataa ggcagtaaag aaagcaagtc ctaatgttaa gtagcccaac
    17701 ggaacacaaa agaacgctgg ttcaatcagc attccgcttg aatcgaggta agaaccgact
    17761 aggatggaag ttgaaaataa caaaatactc aagaaaagaa atatacaagg aataatgaat
    17821 tttttcatgg tgtctccttc tttgtttttc tagattatat agaatagaac ctaggaaaag
    17881 ataagtgtga caaaattgat aggtgtgaaa ggcattgaga tatcaaccag ctttatcctt
    17941 tggagtatgc tattgtgatt aatgagacag aggatgtggg accagacgat actgaatcaa
    18001 cggaaattgt tctaaaccac catcaaatag tagtttttct agtatttgaa ggtgattttt
    18061 ttatgttcga aagtaactgt aaaaaagata aataatcggc agttattctt tcacataagt
    18121 aggttggatg caggtgagag aagtttattg tatgagagga agattggtaa tctaactata
    18181 gttttataac taatatcagt ttaagctcga ttatttttgt ttcaaagtaa aattggaaca
    18241 gaaatataga atagacatat tgtagccgat agtttattat gcttctagac acgttgaact
    18301 ggaggg
//
LOCUS       DAZKCY010000049        18013 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000049 DAZKCY010000000
VERSION     DAZKCY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.049"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..726)
                     /locus_tag="ACG8XC_001528"
     CDS             complement(28..726)
                     /locus_tag="ACG8XC_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFP9191226.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="ACG8XC_001529"
     CDS             complement(719..2071)
                     /locus_tag="ACG8XC_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFP9191227.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="ACG8XC_001530"
     CDS             complement(2294..3694)
                     /locus_tag="ACG8XC_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP9191228.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="ACG8XC_001531"
     CDS             3855..4592
                     /locus_tag="ACG8XC_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFP9191229.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="ACG8XC_001532"
     CDS             4717..5196
                     /locus_tag="ACG8XC_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFP9191230.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="ACG8XC_001533"
     CDS             5193..5960
                     /locus_tag="ACG8XC_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFP9191231.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="ACG8XC_001534"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="ACG8XC_001534"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9191232.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="ACG8XC_001535"
     CDS             complement(6863..7465)
                     /locus_tag="ACG8XC_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP9191233.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..7801
                     /locus_tag="ACG8XC_001536"
     CDS             7571..7801
                     /locus_tag="ACG8XC_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191234.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7792..8040
                     /locus_tag="ACG8XC_001537"
     CDS             7792..8040
                     /locus_tag="ACG8XC_001537"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9191235.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8146..8730
                     /locus_tag="ACG8XC_001538"
     CDS             8146..8730
                     /locus_tag="ACG8XC_001538"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP9191236.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="ACG8XC_001539"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="ACG8XC_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP9191237.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="ACG8XC_001540"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="ACG8XC_001540"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9191238.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="ACG8XC_001541"
     CDS             10631..10810
                     /locus_tag="ACG8XC_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191239.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="ACG8XC_001542"
     CDS             10832..11857
                     /locus_tag="ACG8XC_001542"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP9191240.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /gene="glyA"
                     /locus_tag="ACG8XC_001543"
     CDS             12032..13276
                     /gene="glyA"
                     /locus_tag="ACG8XC_001543"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP9191241.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="ACG8XC_001544"
     CDS             13429..14376
                     /locus_tag="ACG8XC_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFP9191242.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="ACG8XC_001545"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="ACG8XC_001545"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP9191243.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="ACG8XC_001546"
     CDS             complement(15278..15520)
                     /locus_tag="ACG8XC_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191244.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="ACG8XC_001547"
     tRNA            15641..15713
                     /locus_tag="ACG8XC_001547"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
     gene            complement(15832..16470)
                     /locus_tag="ACG8XC_001548"
     CDS             complement(15832..16470)
                     /locus_tag="ACG8XC_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFP9191245.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16593..17225)
                     /locus_tag="ACG8XC_001549"
     CDS             complement(16593..17225)
                     /locus_tag="ACG8XC_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9191246.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17576..17875
                     /locus_tag="ACG8XC_001550"
     CDS             17576..17875
                     /locus_tag="ACG8XC_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9191247.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6165 a         3155 c         3558 g         5135 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggca aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg
    15781 aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa
    15841 taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat
    15901 gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa
    15961 aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa
    16021 tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat
    16081 agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa
    16141 taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg
    16201 ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat atttggctat
    16261 atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac
    16321 aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc
    16381 aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag
    16441 ttctattttt tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa
    16501 gatagagtca gtatacatga agagttaaaa taggcaatgt ttttttatgg atatttgtat
    16561 tgctaaaaat ttatcttata tattcatttt gattatacta cttttatttg taaaaatttt
    16621 ctagcaaaac caagtgcttg caactgatta agcgtacaat tttttagatg ttccgagcgt
    16681 acttcaatca tttcaaagga gttgctactt aaatcgatat tttttaaatc agtatcaaaa
    16741 aagctagcat tttctaaatc attagtagaa aacattattt tagtgaggga acagtctaca
    16801 aaatctgcgc ttgataagac agagtgctca aaggtacttt cttttatgtt tgaaccagat
    16861 aaatttacat agtccatttt acaattctcg aataacgtgt tcccaatata actttccatc
    16921 aatttagtac ctgttaattt acaattgaca aatttcacgc gatgcaacat gctttttgtc
    16981 aatatacagc ctgaaaaatc acagttttcg aatatacagt cggtaatctc cattctttga
    17041 aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat tggatggcag
    17101 attatttggt tttcgatgac ctctttttgt gaaaggatat tatacagttc aaaatcttcg
    17161 ttttcataga tatgaaaaaa ggatccttta ttcaagcttg cttcattaac tttaggaaaa
    17221 atcatattta attacctcca ttttcataaa gtcatattaa caaaagctaa accaacttac
    17281 attagcagaa aatgagatag gttttcgcct ttttcgataa cgatagtgtc gctatctctt
    17341 gaaacgttct tagaattgcc ctgagagaca caaattaggc gtaattctaa taaaaatctt
    17401 agaataaaaa tttttactga tttaatttag aatgttttta aaataaaaat cgttgccata
    17461 acagtgtatt gaaaggtttt aacaatccag taatgattta aaaaaatcta gatgtagcgt
    17521 atactttgtt tatagaatgt agtgggaaga aatatatttt tgggaggaaa tcgtgatgtc
    17581 taaaagtgaa gaaatagaaa acatggtact aaaattatat gatctcgttt taaatccaga
    17641 gatcaaagaa aaggaacgta tacttttgat agacgcaaag acaggcttag aaaaaggtca
    17701 gtattatcct aaagtcataa ataatttgga acgtagttta cgtccgttag caatcagggg
    17761 agaattgtct aaaccagtat caccgtttta tatggagata tcaacaatag gaaaatttga
    17821 aaaagaatta ggtcgaggaa tggcttcggc accgattact tttgggcatt tataaaaacg
    17881 agttattcat ctcaatgaat taaaaagtat agtcaatctc ataatgcagg ctaggttcat
    17941 ttatttgaac agagtctgta tttttttatt ctaaataaag aaacagatgt ttctttatat
    18001 tgtattttat cct
//
LOCUS       DAZKCY010000050        17836 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000050 DAZKCY010000000
VERSION     DAZKCY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.050"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1079
                     /locus_tag="ACG8XC_001551"
     CDS             <1..1079
                     /locus_tag="ACG8XC_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191248.1"
                     /translation="FKENDYLKNKEIIIPEFDSRNILLALVRYVNWEGYENKNPYVEE
                     ACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDKKSAETKPLFQ
                     LVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVYKNFVLNDLSL
                     NELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLSEKEQLLYGKI
                     VEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVSESSVKQFFYR
                     TQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGLETEKTKIYPI
                     SLKGANIHLYHIAQELHLLKKHIS"
     gene            1300..3063
                     /locus_tag="ACG8XC_001552"
     CDS             1300..3063
                     /locus_tag="ACG8XC_001552"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP9191249.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3147..3605
                     /locus_tag="ACG8XC_001553"
     CDS             3147..3605
                     /locus_tag="ACG8XC_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9191250.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3714..4397
                     /locus_tag="ACG8XC_001554"
     CDS             3714..4397
                     /locus_tag="ACG8XC_001554"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9191251.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4544..5158
                     /locus_tag="ACG8XC_001555"
     CDS             4544..5158
                     /locus_tag="ACG8XC_001555"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP9191252.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5174..5380
                     /locus_tag="ACG8XC_001556"
     CDS             5174..5380
                     /locus_tag="ACG8XC_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191253.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     regulatory      5605..5771
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            5919..6476
                     /locus_tag="ACG8XC_001557"
                     /pseudo
     CDS             5919..6476
                     /locus_tag="ACG8XC_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            6534..8573
                     /locus_tag="ACG8XC_001558"
     CDS             6534..8573
                     /locus_tag="ACG8XC_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP9191254.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(8682..9833)
                     /locus_tag="ACG8XC_001559"
     CDS             complement(8682..9833)
                     /locus_tag="ACG8XC_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP9191255.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(9987..10517)
                     /locus_tag="ACG8XC_001560"
     CDS             complement(9987..10517)
                     /locus_tag="ACG8XC_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP9191256.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(10616..10966)
                     /locus_tag="ACG8XC_001561"
     CDS             complement(10616..10966)
                     /locus_tag="ACG8XC_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFP9191257.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(11123..12652)
                     /gene="ahpF"
                     /locus_tag="ACG8XC_001562"
     CDS             complement(11123..12652)
                     /gene="ahpF"
                     /locus_tag="ACG8XC_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP9191258.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(12662..13225)
                     /gene="ahpC"
                     /locus_tag="ACG8XC_001563"
     CDS             complement(12662..13225)
                     /gene="ahpC"
                     /locus_tag="ACG8XC_001563"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP9191259.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(13369..14031)
                     /locus_tag="ACG8XC_001564"
     CDS             complement(13369..14031)
                     /locus_tag="ACG8XC_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9191260.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            14215..15861
                     /locus_tag="ACG8XC_001565"
     CDS             14215..15861
                     /locus_tag="ACG8XC_001565"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9191261.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            15852..16934
                     /gene="uxuA"
                     /locus_tag="ACG8XC_001566"
     CDS             15852..16934
                     /gene="uxuA"
                     /locus_tag="ACG8XC_001566"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9191262.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(17042..>17836)
                     /locus_tag="ACG8XC_001567"
     CDS             complement(17042..>17836)
                     /locus_tag="ACG8XC_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFP9191263.1"
                     /translation="SVDILRPNLPICREQRILVNILARLSITKIESEETSLEQYKLLK
                     KANVPHVLYSREMVESLCTVFDLFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPT
                     FMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQ
                     PRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENCNIVISDCPLKQLPSHI
                     KKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         6084 a         3141 c         3438 g         5173 t
ORIGIN      
        1 tttttaaaga aaatgattat ttgaaaaata aagaaatcat tatcccagaa tttgactcgc
       61 ggaacatctt gctagcttta gtacgttatg tcaattggga aggctatgaa aataaaaatc
      121 cgtatgtaga agaagcttgt aacgagttga ttgattatgt agagactcat ttttttaaaa
      181 gaagatattc ggaagatgaa aagtccttta tcatacgagg aatagagatc gcaatcggaa
      241 gaaaggcatt tccccttgga ctgaaagaag aagataagaa aagcgcagaa acgaagccgt
      301 tatttcaatt agtctgtcaa ggattggaag agcaaaaagg aattctagac ttacaagaag
      361 aagatgtcta ttatattttt tctttgttca acagtagaaa ttataccaat gagaacatgg
      421 agctgttaag aaaagatttc gaagttgttt ataaaaattt tgttctcaat gacctatcac
      481 tgaatgaatt gattttacag attattagta tcgctgaaat cgacgatgca gaaaatttta
      541 ttttcaagca atccttttta caatttgtcc gtacgttgtg ggcagatgga caagtatttt
      601 taccggaaag gatctatctt ctatcagaaa aagaacaact tctgtacgga aaaattgtag
      661 aaatattgga agagtggaaa aagaaaaatc aaatccaact aagatggaat gaaaatttca
      721 ttcgaaaatt cgtcaaaagt ctttctttga tcaacagtga acaagcgggg agacaggaaa
      781 tagaaatttt tgttgtttca gaaagttcag tcaaacaatt tttttatcga acccaatttc
      841 atttgtatgc ggaagaaggc atcgctgaaa taaatcctat tttatttcgt tcattagaag
      901 aattgcctga tgaatgtcta tgtgcgaaaa aaagagtggt tttgtgcgat gctgctagct
      961 atcaaaatgg attagaaact gaaaagacaa agatctatcc aatctcatta aaaggtgcaa
     1021 acatccattt gtatcatatt gctcaggaac ttcacctatt aaaaaaacat atttcttgat
     1081 gagaccttaa ttaggagacc ttctacaaag ctttttaatt gtctgtagaa ggtttttatt
     1141 tgagtcctga ttcgtgcgaa gatctactta tataacaagc tttctctgaa atttttttaa
     1201 aatgctattg acttagagtc aactctaagt agtagaatag ataaagtgta acaaatctat
     1261 tttcattaaa agaagatagt acggaggaga gaatgtatta tgagtacagt attacatgta
     1321 ttggatgcaa tcaatagccc cgcagatgtc aaaaaattat ctgttgagga aatgaaacag
     1381 ttggcagaag agatgcgcca aaaaatcttg caaaaggaca gcgatgtagg tgggcacgtt
     1441 ggtccgaatc taggtgtagt agaattaacg attgcgtttc attatgtatt tgattcacca
     1501 aaggataaaa tcgtttggga tgtttcccat cagtcttatg gtcataagct tttaactggt
     1561 cgaaaaaatg gttttgaaaa aggacattac cgtgaagtga gcggatattc caaccaacat
     1621 gaaagtaaac atgactattt tacagtgggg cacacatcta catctatcag cttagcaaca
     1681 ggtttagcta aagcgagaga tctgaagaaa gaatcaggaa atgtggtagc atttatcggt
     1741 gacggctccc taagtggtgg cttagctttt gaaggattga acaatggtgg tgttttacaa
     1801 tccaatctaa tcgtattagt aaacgataat gagatgtcta ttgatgagaa tcatggcagt
     1861 ttatataaaa acttagctga gcttcgtcaa accaacggta aaagcagcaa taacctattt
     1921 aaagcaatgg gatttgatta tcgttacgtg gataatggaa acgatctgga aacggtgatc
     1981 cgtttatttg aagaagtaaa agatattgat catccgattg tacttcacgt ccatacggaa
     2041 aaaggtcgcg gttatcagcc ggcaatcgat aataaaatga aatatcattg gcatgtacca
     2101 tttgatctgt ccaccgggga ggcaaagcaa gtaacttctg gagaaacatt taatatgatc
     2161 atcatggatt atttggataa gaaaatgcaa acaagtacag ctcctattgc cgcagtcaat
     2221 gcagcgatac caggcgcctt tggactgaaa acattccaag aaaaacatcc agatcattat
     2281 tttgacgtgg ggatcgctga acagcactct atcactttcg cttctgcttt ggcttctcag
     2341 ggagtacgtc cagtcgtctt ccaatcaagt actttcttgc aacgggcata tgatcaatta
     2401 tcacatgatt tagcgatcaa cgagaatcca gtgattattt tagtcggtgg cggaacgatc
     2461 tcagctagtg accgaacaca ccaaggaaca tttgacattc cttacttatc aagtatcccg
     2521 aacatcaaat atcttgctcc agcaacaaaa gaggatatga tctctatgct agattgggcg
     2581 ttcgaacaaa cagcgcagcc tgtagcaatc cgaattccta cacatggtgt agaacatggt
     2641 acagctatcg caaatgatta tagtattcca gcctatcaaa cagtaaataa tggaggaaaa
     2701 gtagcaattt tagctttagg cggttttctt gaattaggaa aagaaacagt aaaagctttt
     2761 agaaaaaaag gaatcgaagc aacgttgatc aatccaggaa cgatcacaga aaatgacgta
     2821 gaaacattag ataaattaaa agaaacacac gaattagtca tcacattaga agatggtagt
     2881 ttgtcaggag gatttggcga aaatatcagt cgttattatg gtgattcttc aatgaacgta
     2941 ttaaattttg gtgcagagaa agaattcacg gatagcgttc ctgtagggca gctttatgaa
     3001 cgttatcatt taacaccaaa acaaatcgtt gaagatagtc tgcgtgtctt gaatattcaa
     3061 taattcatta tgctcaccat ccagataatt gtttatacta gtatctggat ggttttttca
     3121 ttataaaaag caaaggggtg gatgttatgt atcagcaagt agaagaatat gtcaaaaagt
     3181 tccaagaaaa atatcctagt tatcaatcag aaaaattagg tttcttttca tttggggata
     3241 cagaaaaaat ggcagacgag ttgagcgaac tagttgtaaa aaagataaag actggtacat
     3301 caagtggtta cgatctgtat aaacaagaga atgagccttt accaaaagtt ggtcaattag
     3361 acgtggtcct gaatggaaaa aatgaaccag tttgtatcat caaaaatata aatgtgacca
     3421 tcttgccttt tagtgaagtc acgaaagagc atgcttggaa agaaggagaa ggggatcgga
     3481 cattagctta ttggcggaaa gtccatgctg agttttttaa acatgcttat aaagaagcgt
     3541 tgaacataga atttacagaa gagaagctgg tcgtatacga agaatttgaa gtaattttct
     3601 tttagtaaga aaggtctgct gaaagaaata gctcattatt gtctcctgaa tagaaaaggg
     3661 ttacattttt atatgaagct gtatttttat ataacattac tggaggagat attgtggata
     3721 cactggaaca aatcaaaggc ggagtcgtcg tttcttgtca agctttggaa aacgagccgc
     3781 tacacagttc ttttattatg gggagaatgg ctttggctgc aaaagaaggc ggagcagttg
     3841 gtattcgagc aaatacgaca gaagatatca atgaaatcaa gcgaatcgtt gatttaccgg
     3901 taatagggat tatcaaaaga gattacgatg ggtcagaagt gtttattact gcgacagaaa
     3961 aagaagtgga agaacttttg gagacgcaag cagaaatcat tgcactggat gcaacttctc
     4021 gtaaacgtcc gacagaaaaa acaactgctg aacttgtcca aatgattcac gaacacggtc
     4081 ggttagctat ggcagatatt tcgacttttg aggaagcgct tcaagcgcaa gaagatggtt
     4141 ttgatctttt gtctacgact ttagcaggct atacagaata ttcacgaaag acgcagacac
     4201 ctgattttga tttattgaat caaatcatag aacaagtcga tatcccagta attatggaag
     4261 gacatactga ctctcctgaa caagtagaga aagcatatga attaggtgct ttttcagtcg
     4321 tagttggatc tattatcacc cgtccgcaat tgatcactaa aagatatgtc gaagccgcaa
     4381 gtaaagcaaa taactaaaaa acacgcacgg cacagattct tgacaaaaaa gaaaaattga
     4441 tagatagttt aaagcagatt ctaagaagat gaaaaaagca ttacttttca ttttgtcttc
     4501 aagatctgct ttttaacgaa ttttatagaa agaaggcgaa accatgtttg aacggagtcc
     4561 ttcagaattg acagaacgtg aaaattacaa gttgctgatt ggttcagtca tccctagacc
     4621 agtagcagta gtaacgacta aatcagcaaa agatatagtg aatatcgcac ccttcagtta
     4681 cttcaatatc gtcagttcga atcgacccat cttatccttg gcgatccaga gaaaacaaga
     4741 tgcagtaaaa gacacagcaa agaatattct tgagacaaga gaagctgtta tccatatcct
     4801 tgatgaggac aacgtagaag aagtaaataa aacagcagcc aatctgccta atgaggaaag
     4861 cgaactgtac ctgactgact tgactttaac tgattcagta atcatttcag taccagcatt
     4921 gacagaagca aaagttcgtt ttgaaacatc cttatatgaa catgtcgaga tacggaataa
     4981 aaataaagta acagctgatt tatttttgct agaagtacag aaatatcatt taaatgaaac
     5041 agtctatgat gataagacag gaaggatcga tccaagaaaa ttgaatgctg taagccgttt
     5101 ggcaggaaat gattatgcag caataggaga aattttcacg attgcacgtc ccgaataaaa
     5161 aggagcacta gttatgttaa aaataagcta taaaccatcg acagattcaa aagaaatgaa
     5221 aaaagaatat gaaacagtga atgattttct gcaagggcag tacttagaag ttccgccatt
     5281 gcaagaccat tttgtcgtta ctactgttac tttagatggt aaggaaatag aaatgccaga
     5341 tcaaacgatt agcggattgt ttaattactt taacaaataa atatttattc cgaaaagtga
     5401 aaaaacatat aaaaaaacac ataaaactaa aaaattatca aagaatcctg gtgcaactga
     5461 aagaatttgc aacgggattc tttcaactta tttgttattt ttttctaaaa aatattaaag
     5521 attgttaaat ttcactattt catttcaaat tccattgcct aatcattagg aaaagtctat
     5581 catgaactcg taaatagaat atagtaataa ccttgttagg tgaggctcct atacaaacat
     5641 aggctgttgc ccagaaacgt cgaaagtcgc caatgggtaa aacagaaact gttggattaa
     5701 ggctttttcc aatagctaag aatgttttct ttacgttgta tagtgctaaa actcggacga
     5761 tgagatgatg ctaggtcaga attcttgttc gaaaaggttt ctgacctttt ctttatattt
     5821 ttgtgactga cttatttgat gagacaaact cgctcctcac ctgacaagtg gattgctgtg
     5881 tgaggaggag atgcggtgaa aaaagatcga ttggtggagg tcgctcggat tcacttctgg
     5941 ttgcacaata caaccagaaa acacaacaag caaaactgat ctctatcatg agagattcat
     6001 atgtcgatat tccaggttac ggaatgaata agataaatgc agcttactct tatgggggag
     6061 ttgatttatt aaatcaaaca ttaaaggaaa atttcaaatt tgaagctccc tattatgcaa
     6121 gtatcacatt tcaagatttt atcgattgcg ttaatgaact gtttcctggt ggagtcaaga
     6181 tcgatgcaga aaaatctcta gatttagatg gcgtatatat aaacaaagga gagcaagtga
     6241 tggatggtaa tactttactc cagtatgctc gatttcggga agacaaagaa ggagacttcg
     6301 ggaggatcag aaggcagcag caagtgatca aagctatctc tcagcagctg aaagacgtaa
     6361 cttcaatatt caagctaccc aaagcggttg ggaaattact tggtagtatc caaacgaacc
     6421 ttccagaaag tgtgcttctg gattgtggta tggatttcct caaagacata tgttagaata
     6481 aacaggtata aatagtgaaa taaaggaatg acaagcaaga gaaggaggaa aaaatgaaaa
     6541 gaagtgacaa gcacggcaaa aatcgaacag gcgcttatat tgccggcgca gtgatcttaa
     6601 tagcagctgc gggtggcggt tatttttact accagcacta tcaagaaacc caagcagtag
     6661 aagctggaga aaagacagtt gagcaatttg tccaagcttt gaacaaggga gattataaca
     6721 aagctgcaga aatgacatca aaaaaggcgg caaataaaag tgcattatct gaaaaagaga
     6781 tcttagataa ataccagaat atatacggtg ctgctgatgt caaaggactt cagatatcga
     6841 atctaaaagt agataaaaaa gacgattcta cttacagttt ttcatataaa gcaaagatga
     6901 atacctcatt aggtgaattg aaagatcttt cttataaagg aacattagac agaaatgatg
     6961 gtcaaaccac gatcaactgg caacccaact tggtttttcc agaaatggaa ggaaatgaca
     7021 aagtaagcct gaccacacaa gaagcagcaa gagggaatat tatagatcga aatggtgaac
     7081 cattagcaac aacaggcaaa ctaaaacaat taggagtcgt tccaagtaaa cttggagatg
     7141 gaggcgaaaa aacagccaat atcaaagcga ttgcttcctc cttcgactta acagaagatg
     7201 ctatcaatca ggcgatttca caaagctggg tacaacccga ttactttgtc ccattgaaaa
     7261 tcattgatgg agcaacgcca gaacttccag ctggagctac cattcaagaa gtagacggca
     7321 gatattatcc tttgggtgaa gcagctgctc agctgattgg ttacgtggga gatatcacag
     7381 cagaagatat tgataagaat ccagaattaa gcagtaatgg aaaaatcggt cgctcaggtt
     7441 tggagatggc ttttgataag gatcttcgtg ggacaacagg tgggaaatta agcattacag
     7501 atgcagacgg tgtagagaaa aaagttctga tcgaacatga agttcaaaat ggcaaagata
     7561 tcaaattgac aatcgatgcg aaggcacaaa aaatagcttt tgacagtcta ggaggaaaag
     7621 ctgcatcaac tgttgcgaca acgccaaaaa ccggtgatct tcttgcgctt gctagctctc
     7681 caagctatga tccaaacaaa atgacaaacg ggatctcaca agaagattac aaagcttatg
     7741 aagaaaatcc tgaacatcca ttcatcagcc gatttgcgac aggttatgct cctggatcta
     7801 cgtttaaaat gattacagca gcaatcggtc tcgacaacgg cactatcgat ccaaatgaag
     7861 tgttgacgat caacgggctt aaatggcaaa aagacagttc ttggggatct tttaaaacaa
     7921 cgcgtgtcag tagtgatgta tcacaagtag acttaaaaac tgctttgatc tattccgata
     7981 atatatatgc ggcacaagaa acgttgaaaa tgggtgagaa aaaatttcgt acaggcttag
     8041 ataaattcat ttttggtgaa gaccttgatt tgccaattag tatgaatcca gcacaaattt
     8101 ctaatgaaga tagctttaac tcagatatct tgctagctga tactggatat ggacagggcg
     8161 aacttctaat tactcctatc cagcaagcag caatgtattc tgtttttacc aacaatggca
     8221 cacttgtcta tcctaaattg attgcagata aagagacaaa agataagaag aatgtcatca
     8281 gcgaaacagc agtacaaacg atcgtgccag atctgagaga agttgtgcaa gatgtaaatg
     8341 gtacagcaca ttctctttct gctttaggga ttccgttggc agcgaaaact ggtacagcgg
     8401 aaatcaaaga aaaacaggat gtaaaggggc aagagaacag tttcttgttt gctttcaatc
     8461 ttgataacca aggttatatg atggtcagca tgttggaaaa taaagaagat gatgattcag
     8521 caaccaaacg agcatcggaa ctattgcaat acctcaacca aaattatcaa taaaaaattg
     8581 ctttttaagt acagtaaaag gactgtggcg ttatcgtcac agtccttttt tcaaataaac
     8641 ggttttcacg atgtctcttt cacaacttct ttttgattta ctcacacttc ataaactttt
     8701 tttgtaaaat atttgagaac aagtggagaa ataagtgtac ttaataaaat cacgataact
     8761 aaaggtgaat agtactgtgg ttcgatcaat tgactttgct gtccaatttg taaaatgatc
     8821 aaagccattt ctccacgcga aatcatacca gcaccgacca tcaatgaact atgttgagaa
     8881 aaaccggcaa tctgagcacc aaaataacct ccaagcaatt tggtcaaaat cgctacaagc
     8941 gtcaatatag aaataaagag tagttgttca ctaaagtcag aaaaatcaac ttctaatcct
     9001 acactgacaa aaaatactgg tataaatact gcatatccta aagcctctac attgtggtat
     9061 acttcttctt taacctttgt ctgactgaca gctatcccag caaaaaaagc accaatgaca
     9121 gagctaagcc caacaagatc cgctacgtaa gacattccta aacaaataac tagcgatacg
     9181 atgataatag aagaatttgc gtacactttt tctgcaaatg aaagtaggaa aggcgcaatc
     9241 catttgacta ataagaaaat aaaaacaaaa taaattagct gttcaatcaa aacaagcggt
     9301 aaagaagcat tgctagaagc ttcccccgtt agaaaagata ggctgaaact tagaactaaa
     9361 acgactaaaa tgtcatctac cacagaagca cccaaaattg ttgatccttc ttttgtattc
     9421 actacgttta gttcttttaa tacttctact gaaatactga cagaagttgc cgcaagaata
     9481 atcccaaaga aaatcgcttc atttcccgaa acttggaatg ctttgcctgt caaccagcca
     9541 aaaacaaccg ggaagatgat cccaagcaaa gcaacgaaca taccaggtcg gaaatatttt
     9601 ttcaaaagag ataaatcact ttccagacca gctaaaaaca ttaataagat cacaccaatc
     9661 tccgaaaagt catggactag aatatcaggt ttgacccaat tcaatccagc ttggcctagc
     9721 aaaacaccaa caagtaattg tccaatcact gctgggatac ctaatctccg cgagatatgt
     9781 gagccaatcg ttgttgaaat caatatcagg cataaaatac caataaattc cattctcatc
     9841 attcctttgt ataaaatata gcccgaactt agcataacat acattattga aaaaatagga
     9901 aaatatactc acaaaaaaac caacaatcca aaagattttt ttggattgtt ggtttttata
     9961 cggtctattt taataactaa aaatgattaa cgtatagtca tttttcttat tctgatgaaa
    10021 ttgataatct cctggatgcc catgaaaatc aaaataaagc cgaatatttg gaatagaagc
    10081 atcaaacttt tgaatggatt gaacaataag acgaatccgc caatcaacaa cagaatacta
    10141 ctataaacac tccaactttt agcttttgta ttcatgctga agaacaactg gaacaagctg
    10201 ttgatgataa cagctaatcc aagaagaatc ggcaggatag aaacgattgc aggtgcaaaa
    10261 gcatagacga tcagagctaa gatcaggaaa aaaagaccac tgaacagacc gatactccaa
    10321 gaacctgttt tccttttaaa acggtaagcc tcaaaaagat tcaacaaccc aagcaaaaca
    10381 agataggcgg tgatcagata accgataaaa tgaaaaacag cagaagggtt tagcacaatc
    10441 gcaatcccag aaataatata aacagcagct cttaataagg cgtatttttg aatattttgt
    10501 ttaatcgtct caaacatata agaaaacctc ctttgactta ttttaacgtg aactagtcat
    10561 tttaccaaat taaatgttac aaaatgagcg aaggagaaga atatctctgg aaaaatcaac
    10621 ttctgaggat cttttccatt tttttgcctt ttgctagctc atcgatcatt ttatctaaat
    10681 aacgaatttc tttcatcaag ttatcctcga tgtcttctac tcgtacacca caaacaacac
    10741 ctgtaatcaa ggaacgtgaa ggattgggga caggggcttc cgcaaaaaat tgttcgaaag
    10801 tcacttgttt ttgtagttga tattctagtt ctgtttggga ataacccgtc agccaacaaa
    10861 tgatttcatc tacttcctct tttatacgat cttttctctc cgcttttgct acatacatag
    10921 gataaacgct tgcaaaactt gtcgtaaaaa tacgattttg cgccataatc agtcaccttt
    10981 ctttttttca aattatttac tacttatagt atataagaaa aagagaagaa aaagcgatta
    11041 ttcaaaaaaa caaaaaggat tgtgacaaaa ttcttgtcac catccttttt tccgctctct
    11101 gactttttac aatcttcata gctcaagtgc gcatcagata atcgaatgct cctaaagctg
    11161 cagtagctcc tgaacccatt gaaacaatga tttgtttgta tggactatct gtacaatcac
    11221 cagctgcgaa aacgccgggg atattcgttg cgccattttt ctcggtcact atttcatttc
    11281 ttggattcat ttcgatcgtt ctatctagcc aatccgtatt agggagtaag ccgactagaa
    11341 tgaatactcc tgaaacagct agtgtatgca tctccatcgt acggcgatcg atataggaga
    11401 gagattctac ctgattcgta ccgctgattt ctttcgtcgc tgcatgtttg ataacagtga
    11461 cattcgcaag agagactagt ttatcctgta agatttgatc tgcttttaat tctggtaaga
    11521 attctagaac atatacatgt tttgctaacc cagccaaatc gattgctgct tcaacaccag
    11581 aattaccacc accgataaca gcaatctctt ttcctttaaa taaagggccg tcacagtgtg
    11641 ggcaataagc aattccttta tttttaaact ctttttcgcc tggtacattg atagaacgcc
    11701 agtgcgcacc tgtcgcgatg atggcggttt ttgctttaag tgatataccg ttagctaagc
    11761 caatattaat aaaatgttgc tctttattta aggaaacggc ctgttgattc atcataatgt
    11821 cgatagggta actacgcaca tgactttcta cttgctgcat caattttgga ccttctgtgt
    11881 aagaggtacc aatcatattt tcaatcccta gtgtttcaac gacttgtccg ccgaaagaat
    11941 cagcgacaag tcctgtacgg attcctttac gtgctgcata gatagctgca ctactagctg
    12001 ctggtccacc gccaatcact aatacatcat aaggttcttt ttcaaacaac tcttcttcaa
    12061 tcaaagggcc agtgattttt tcaacaattt gttcgatcgt catgcgtccg ctactaaatt
    12121 cttctccatt taaaaacaca gttggcactg ccatgatttt tttatcatcg atttcttttt
    12181 ggaacatccc accttcaatc atcgtatgag taatatttgg atttaataca gctaatatat
    12241 tcaatgcctg tacaacgtcg gggcaattat gacaagtcaa gctaacataa ctgtcaaaat
    12301 gcaatgtttg tgttattttt ttgattttaa gactcaaaga ttcactgatt ttcggtgccc
    12361 gtccactaat ttgtaacata gccagtaaaa aagaggaaaa ttcatgaccg agggggatac
    12421 cagcaaaaac gattccactt tctttgccaa ctggattgat agaaaaacta ggtgtgcgca
    12481 tcgaatctct tttttcccaa gagattttag gagacatttc tgctatttcc ttcgtaaatt
    12541 cagctagttt ttgactattt tcactattat ccaaactaac agaaaaaaca ataggagatt
    12601 caatcagtgc taaatattgt tcgagatttt gtttagtttc tttgtctaac atatccacca
    12661 cctaaatctt tccaacaaga tcaaaacttg gtttcaatgt ttcgccactt tctttccatt
    12721 ttgccggaca aacttcacct gggtgggtac gtacatattg agcagcgcga atcttatcaa
    12781 tcagtgcact agcgtcacga ccgattccat ctgcattgat ttccatagct tgaacgattc
    12841 cgtctggatc aataataaat gttcctcgct gagctaatcc ttgctcttca tccaaaacat
    12901 caaaaatacg agaaatctga tgagacggat caccgatcat cacgtattcg attttaccga
    12961 tagcatctga tgtatcatgc catgctttgt gagtaaagtg ggtatcggta gagcaagaaa
    13021 agacttcaac acctaaagat ttcaatgtac tgtattgttc ttgtaagtct tctaattcag
    13081 taggacaaac aaaagtaaaa tcagctggat agaagcaaag aatactccag ttccccataa
    13141 gatctttttc actgacttct aaaaattctc cttgatgata agcatgtgca ttaaattctt
    13201 cgattttttt cccaatcaat gacatagtaa aacctccaat aataattatt tatttataat
    13261 tattgtaatt tagttggagt tgcaaagtca atggatattt tgttatttaa taaagattac
    13321 tcaaaaacga aggttaatta taaaaataga agaggtcgca agaaaatgct actgctggaa
    13381 atactcggga taacgttgag taacagtttc ctgttctttc agcatcatgt gtaagtgcaa
    13441 tcccgtttga aatcgagctt cttctggttt tttatcaact aaagccttga gtaaatcata
    13501 atgttgttcc atgatcgttt cccatggaag cccctgaatg gtcactcgta accagcggaa
    13561 acgttctaaa tgggtattat ggtcatctaa ccagttccaa acttcttttc ttcctgcaat
    13621 ttcgaaacaa gcttcatgaa ataaattatc tgcacggaaa aaagcgcgtt cgtcatttat
    13681 tttgactgct ttttcttgtt cttctaaaat cgtttctagc actattcgtg tttgtgtgtc
    13741 caatttggaa caacattcaa tcatgatttg ctcttccaac tgttctcgga caaagcgagc
    13801 attttctgca gaaacaagat taattttgga aacatacgta ccactttgag gtatcgtata
    13861 cacgagttct tgctgacgta gttgaatcaa tgcttcgcga atgggagtcc ggccgatagc
    13921 aagatgttcc tccagtgcct tttcagatat tttccttcca ggttctaagt cagcatagat
    13981 aatttgttta cgaatcgctt gataagcttg tgattgcaag ctgcttttca tctttattcc
    14041 tccaaaatca tttctatgaa cgtagtataa cagaagaggt ctgaaaaaca atctggtatg
    14101 tcacattaac tagtaaattt gtataaatag taaaattttg gttgacatag cgatttttat
    14161 tgcataaaat tcacttgaat acaaaaacta atatgttagt ttaggagggg aaaaatgatt
    14221 gatttaaaca actggaggaa ctttatccct gaattatctc aaaaagaagt agaaatacct
    14281 cattttgacc gtgcatcatt aagagaaaat ggacaaaaaa atcctgtctg gatccatttc
    14341 ggtggcggaa atctttatcg aggatttcat gcagaaatcg ctcaacgttt agcggatcta
    14401 ggcgaattgt ccactggtgt cattgtttgt gaaacattcg atgaagaaat aattgaaagg
    14461 gcttaccaag attttgatga tttgattctt gaagtggtca tgccaagcag tggagagttc
    14521 gtcaaacgta tattagctgc gacagctgac agtctgtatt gtcatccaga ttgttctgaa
    14581 cactttgata agatgattcg acactttgaa aatcctagtt tacaattagt tacttttgct
    14641 attactgaaa aaggttatcg attgaaagat acaaataata aatatatagg accgattgca
    14701 gtcgatataa aaaatggtcc aaagaagcca tcacatacaa tgagtattct tgctgtgcta
    14761 ctttataaac gttttttatc tggagcttgg ccgattgcac ttgtctctac agataatttt
    14821 tcacaaaacg gtagtcaatt acgtacttct ttgctaacga tcgcaaaaga atggcaaaag
    14881 gaaggttatg tacaaaatac atttatccaa tacttatcga atgaagaaat tgttgcgttt
    14941 ccatggacga tgattgatcg tatcaccccg aatccttcct tgtcagttag caaaatgttg
    15001 gcaaaagagg gcttttcaaa tatgtcgatt ttccaaaccg caaaaggaaa taatattgct
    15061 ccatttgtca atacagagga catccattat ttaatcatgg aagatcattt tcccaatgga
    15121 cgtccgaaat tcgaaaaagc tggggtattc ttgactgatc gtgcaacggt agataaagca
    15181 gatacgatga aggtgacgac ttgccttaat cctttgcata cgtctttagc tattatgggt
    15241 tgtttactgg ggtataggag catagctgag gagatgaaag atccagattt atcgctgcta
    15301 gtttctgaaa tcgggtacaa tgaaggcctc ccagttgtgg agtctccagg tatcattgat
    15361 ccaaagatct ttctggaaga agtaattact gtgcgctttt ctaatccaaa tattcctaat
    15421 atgcctcagc gaatcgccac ggatacctct caaaaggtgg cgattcgtta tggtgagacg
    15481 attaaaaaat atatggaaat agaagttaaa gacccgcaga cacttgtatt cataccattg
    15541 actctcgctg cttggcttcg ataccttatt ggcattgatg atcaaggaaa accattcatc
    15601 ccgagtccag acccactatt gacagacctt cagcataagc tttccgatat tcaactgggt
    15661 acgcaagata catcacatat tcatgagaaa atggctccta ttttgtcaaa taaagaaatt
    15721 tttggtgtag atctttacca agcagagctt ggcagaaaaa tagaagaaat gttcatcaag
    15781 atggtagcag gaccaaatgc cgtacgaaaa acattacagg attacttaca agaacatgga
    15841 ggaaatttat catggaaatg acttttagat ggtacggatc acaggaagaa aagattacct
    15901 tagatgaaat cagacagatt cctgcggtca aagggattgt aggcacattg atggatatac
    15961 cagtaggaga ggtatggcct agggagagga tcaaagaact aaaagaagaa attgagtctc
    16021 gtgggttaac actaaaagta attgaaagcg tcaatgtgca tgatgccatt aaaattggat
    16081 taccttcccg tgagcagtat atcgaaaact ataagcagac aatccgaaac ttggcagaat
    16141 atggaataga agtcatctgc tataatttta tgcctgtatt tgactgggtg aaatctgacc
    16201 ttgactaccg cttagaagac ggctcatcca cgttagcttt tatatctgat gatattccag
    16261 atgatccaag agaaatcgtc gcgcgcatcg aacattccag caatgaattt ggattgccgg
    16321 gatgggaacc tgagcgctta gctcctatca aatccttgct tgaagcctat gcatcagtag
    16381 acgaagaaaa acttcgagaa aattttgcct attttctcca atcaatcatc ccaacatgtg
    16441 aagaggtagg ggtcaaaatg gctgtacatc cagatgatcc gccatactcg atttttggat
    16501 tagctcgaat cgtaaagaat cgagaagatt tagattggtt gtgtcgcata gtagacagcc
    16561 cttataacgg aatcactctt tgtacaggga gtattgcaga agatccagaa aatgatgtat
    16621 atgggatttt agcagaattt tgccaaagag atcgaatccc atttgcacat gttcgaaata
    16681 ttaaattttt agctaatcga gatttctatg aggcaccaca taaatccgaa tacggttcat
    16741 tagacatgta ccgcatacta aaaacaatgt atgacaacgg atttaacgga tacatccgtc
    16801 ctgatcatgg aagaatgatc tggaatgaga caggaagacc aggatacggc ttatatgatc
    16861 gagcattagg tgctgtttat ctaaatggat tatgggaagc aatcgaaaaa ggaaacaaaa
    16921 gtaaagataa ttagtgaaaa aaactgtaat tttattatgt aaataagaag gatgttcaaa
    16981 ttcttgatac ataaatccgg aggttgttaa taagcattcg gatttatgta tcttatttgg
    17041 tctaggaagt aacttgattt tcttttttcg attgatccat gagtgcttct tcttttaatt
    17101 caaagatata ttctgagatc ctcataatca cttttttcca gtcttctggt gtgatgattc
    17161 cttttaaaat atagattttt ttgatatgag aaggcaattg tttcaaaggg cagtcgctga
    17221 taacgatgtt gcagttctcc ataaaatcat tttgtatttt gatcgattca tttgaaaaaa
    17281 tttgttctat cttaacgatc agatctttac tgatataaaa atcacgcgta tagttcacac
    17341 caatcacaat tcgcggctgt tttttgtagc gatcatacaa atgtactagg tcttcaacca
    17401 tccattgcac attttccttt tctaaaataa ctggtaacaa gtgttgattt tgttctctga
    17461 aatccatata aaattgttca atggcggttt ggacttcttt caatacgcta tgttcttgat
    17521 aaagcatgaa agtaggaagg ggcgtatttg gttgattgaa gttaagaaat ccattataaa
    17581 gtttatttcg tgcgaatgcc aatgagtaaa gaatcttttc tttttcaggt ataaacaaat
    17641 caaatacagt acaaagggac tccaccattt ctcttgaata aagaacatga ggaacgtttg
    17701 ctttttttaa aagtttgtat tgctccaagc ttgtttcttc cgattcgatt ttcgtgatag
    17761 aaagtcgagc taaaatattc actaagatgc gttgttcgcg acaaattggc agattaggtc
    17821 gcaaaatatc tacaga
//
LOCUS       DAZKCY010000051        17399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000051 DAZKCY010000000
VERSION     DAZKCY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.051"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            264..2030
                     /locus_tag="ACG8XC_001568"
     CDS             264..2030
                     /locus_tag="ACG8XC_001568"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFP9191264.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2132..3391
                     /locus_tag="ACG8XC_001569"
     CDS             2132..3391
                     /locus_tag="ACG8XC_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9191265.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3538..4839
                     /locus_tag="ACG8XC_001570"
     CDS             3538..4839
                     /locus_tag="ACG8XC_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9191266.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4842..5693
                     /locus_tag="ACG8XC_001571"
     CDS             4842..5693
                     /locus_tag="ACG8XC_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP9191267.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5706..6521
                     /locus_tag="ACG8XC_001572"
     CDS             5706..6521
                     /locus_tag="ACG8XC_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFP9191268.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6672..7838
                     /locus_tag="ACG8XC_001573"
     CDS             6672..7838
                     /locus_tag="ACG8XC_001573"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9191269.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8068..8715
                     /locus_tag="ACG8XC_001574"
     CDS             8068..8715
                     /locus_tag="ACG8XC_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP9191270.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8799..9743
                     /locus_tag="ACG8XC_001575"
     CDS             8799..9743
                     /locus_tag="ACG8XC_001575"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFP9191271.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9878..11329
                     /locus_tag="ACG8XC_001576"
     CDS             9878..11329
                     /locus_tag="ACG8XC_001576"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFP9191272.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11350..12186
                     /locus_tag="ACG8XC_001577"
     CDS             11350..12186
                     /locus_tag="ACG8XC_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9191273.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12186..12845
                     /locus_tag="ACG8XC_001578"
     CDS             12186..12845
                     /locus_tag="ACG8XC_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9191274.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12971..13660
                     /locus_tag="ACG8XC_001579"
     CDS             12971..13660
                     /locus_tag="ACG8XC_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191275.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13666..14622
                     /locus_tag="ACG8XC_001580"
     CDS             13666..14622
                     /locus_tag="ACG8XC_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFP9191276.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14666..15178
                     /locus_tag="ACG8XC_001581"
     CDS             14666..15178
                     /locus_tag="ACG8XC_001581"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9191277.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15434..15982)
                     /locus_tag="ACG8XC_001582"
     CDS             complement(15434..15982)
                     /locus_tag="ACG8XC_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9191278.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16071..17252)
                     /locus_tag="ACG8XC_001583"
     CDS             complement(16071..17252)
                     /locus_tag="ACG8XC_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFP9191279.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         5728 a         3092 c         3479 g         5100 t
ORIGIN      
        1 tatacttcta cgttaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
       61 cgcttaagat aaattcagga cactaaatag tacaggaagt agattaaaac ttttaaaagt
      121 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
      181 cgataacatt tttgaaagcg ttgtcttttg tttctctatc acttatagtg atgacgtcat
      241 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
      301 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
      361 atatcaaaaa agtatatgtg ctttgtggag atccatatac cataacaact gaaaaatggt
      421 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
      481 ttgaagtgac gagcgaaaca aggcgcttac agtatgcgtt ccatgttgtt ggggaagata
      541 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
      601 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
      661 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
      721 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
      781 tcttcggggg agatttgcaa ggtgtgaggg atcatttgga ttacttgacg gaccttggcg
      841 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
      901 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
      961 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
     1021 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
     1081 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
     1141 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
     1201 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
     1261 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
     1321 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
     1381 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcattcacag
     1441 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
     1501 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
     1561 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
     1621 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
     1681 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
     1741 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
     1801 ctaagattat ttctgaaggt tctactcagt ggctaatagt atatgatcgt gaagataagc
     1861 tctatttcac acgggaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
     1921 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
     1981 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
     2041 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
     2101 cagcacgttg aatacacagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
     2161 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
     2221 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
     2281 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
     2341 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
     2401 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
     2461 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
     2521 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
     2581 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
     2641 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
     2701 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
     2761 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
     2821 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
     2881 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
     2941 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
     3001 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
     3061 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
     3121 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
     3181 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
     3241 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
     3301 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
     3361 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
     3421 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
     3481 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
     3541 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
     3601 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
     3661 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
     3721 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
     3781 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
     3841 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
     3901 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
     3961 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
     4021 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
     4081 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
     4141 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
     4201 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
     4261 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
     4321 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
     4381 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
     4441 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
     4501 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
     4561 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
     4621 ttctccatga tctatctgtt taacaatggt ggtcccggaa gcgtaggcgg aggagctggt
     4681 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
     4741 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
     4801 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
     4861 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
     4921 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
     4981 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
     5041 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
     5101 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
     5161 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
     5221 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
     5281 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
     5341 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
     5401 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
     5461 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
     5521 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
     5581 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
     5641 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
     5701 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
     5761 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
     5821 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
     5881 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
     5941 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
     6001 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
     6061 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
     6121 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
     6181 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
     6241 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
     6301 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
     6361 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
     6421 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
     6481 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
     6541 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
     6601 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
     6661 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
     6721 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
     6781 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
     6841 cttctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
     6901 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
     6961 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
     7021 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
     7081 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
     7141 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
     7201 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
     7261 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
     7321 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
     7381 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
     7441 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
     7501 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
     7561 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
     7621 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
     7681 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
     7741 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
     7801 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
     7861 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
     7921 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
     7981 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
     8041 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
     8101 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
     8161 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
     8221 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
     8281 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
     8341 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
     8401 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
     8461 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
     8521 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
     8581 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
     8641 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
     8701 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
     8761 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
     8821 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
     8881 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
     8941 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
     9001 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
     9061 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
     9121 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
     9181 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
     9241 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
     9301 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
     9361 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
     9421 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
     9481 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
     9541 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
     9601 gcatgttaca gattggtaca gagcttcata aagaagggcc ggaaattttt tctagaatca
     9661 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
     9721 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
     9781 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
     9841 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
     9901 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
     9961 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    10021 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    10081 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    10141 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    10201 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    10261 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    10321 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    10381 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    10441 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    10501 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    10561 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    10621 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    10681 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    10741 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    10801 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    10861 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    10921 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    10981 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    11041 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    11101 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    11161 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    11221 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    11281 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    11341 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    11401 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    11461 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    11521 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    11581 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    11641 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    11701 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    11761 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    11821 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    11881 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    11941 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    12001 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    12061 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    12121 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    12181 gagtgatgta ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac
    12241 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg
    12301 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    12361 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    12421 tcgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    12481 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    12541 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    12601 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    12661 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    12721 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    12781 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa
    12841 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    12901 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    12961 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    13021 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    13081 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    13141 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    13201 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    13261 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    13321 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    13381 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    13441 aaaatatttt ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    13501 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    13561 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    13621 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    13681 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    13741 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    13801 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    13861 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    13921 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    13981 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    14041 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    14101 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    14161 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    14221 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    14281 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    14341 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    14401 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    14461 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    14521 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    14581 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    14641 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    14701 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    14761 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    14821 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt
    14881 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    14941 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    15001 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    15061 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    15121 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    15181 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    15241 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    15301 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    15361 ccttcatcga gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta
    15421 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    15481 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    15541 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    15601 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    15661 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    15721 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    15781 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    15841 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    15901 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    15961 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    16021 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc
    16081 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    16141 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    16201 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    16261 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    16321 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    16381 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    16441 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    16501 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    16561 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    16621 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    16681 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    16741 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    16801 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    16861 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    16921 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    16981 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    17041 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    17101 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    17161 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    17221 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    17281 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    17341 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaa
//
LOCUS       DAZKCY010000052        17362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000052 DAZKCY010000000
VERSION     DAZKCY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.052"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..484)
                     /locus_tag="ACG8XC_001584"
     CDS             complement(<1..484)
                     /locus_tag="ACG8XC_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP9191280.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     K"
     gene            814..1419
                     /locus_tag="ACG8XC_001585"
     CDS             814..1419
                     /locus_tag="ACG8XC_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFP9191281.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            1744..2067
                     /locus_tag="ACG8XC_001586"
     CDS             1744..2067
                     /locus_tag="ACG8XC_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFP9191282.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            2249..3619
                     /locus_tag="ACG8XC_001587"
     CDS             2249..3619
                     /locus_tag="ACG8XC_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFP9191283.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(3749..5050)
                     /locus_tag="ACG8XC_001588"
     CDS             complement(3749..5050)
                     /locus_tag="ACG8XC_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9191284.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(5280..7271)
                     /locus_tag="ACG8XC_001589"
     CDS             complement(5280..7271)
                     /locus_tag="ACG8XC_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFP9191285.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(7415..7681)
                     /locus_tag="ACG8XC_001590"
     CDS             complement(7415..7681)
                     /locus_tag="ACG8XC_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9191286.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(8191..10317)
                     /gene="pnp"
                     /locus_tag="ACG8XC_001591"
     CDS             complement(8191..10317)
                     /gene="pnp"
                     /locus_tag="ACG8XC_001591"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP9191287.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(10505..10774)
                     /gene="rpsO"
                     /locus_tag="ACG8XC_001592"
     CDS             complement(10505..10774)
                     /gene="rpsO"
                     /locus_tag="ACG8XC_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP9191288.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            11025..11588
                     /gene="def"
                     /locus_tag="ACG8XC_001593"
     CDS             11025..11588
                     /gene="def"
                     /locus_tag="ACG8XC_001593"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9191289.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(11841..12362)
                     /locus_tag="ACG8XC_001594"
     CDS             complement(11841..12362)
                     /locus_tag="ACG8XC_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFP9191290.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(12365..13207)
                     /locus_tag="ACG8XC_001595"
     CDS             complement(12365..13207)
                     /locus_tag="ACG8XC_001595"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFP9191291.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(13200..13925)
                     /locus_tag="ACG8XC_001596"
     CDS             complement(13200..13925)
                     /locus_tag="ACG8XC_001596"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFP9191292.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            14101..14397
                     /locus_tag="ACG8XC_001597"
     CDS             14101..14397
                     /locus_tag="ACG8XC_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191293.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      14653..14827
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            14965..16557
                     /locus_tag="ACG8XC_001598"
     CDS             14965..16557
                     /locus_tag="ACG8XC_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9191294.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            16570..17310
                     /locus_tag="ACG8XC_001599"
     CDS             16570..17310
                     /locus_tag="ACG8XC_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9191295.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         4979 a         3551 c         3338 g         5494 t
ORIGIN      
        1 gcttacattg atacccatca gcgaatacga ttccattttc aaatgctgtt acgtctgccg
       61 gtgtcaccct ttcgttcaca actacttgat aagtctttga aacctttttt tctggaagta
      121 gcatttcata tcccaattgt ccattcgaag tgatcagcaa tagtcctgtt gtatctctat
      181 ctagtcttcc cactggatat aatggttgga aacgatcggc acttgcaatc agatcgatta
      241 ctgtcgtctt ttctggatct gatacagctg taacagcttt ttcaggttta ttgagaagat
      301 aataggactc atttgtttct aagtgttcac ctgctacttc gatttgatgc agtaaactat
      361 ctacatttcg attttgtcga aattcgactg tgccatctat catcacttgc ttcaaagcaa
      421 acagccgctt catttccttg cgtgtcgttt ctaatttttc ttctattaat ttatctaaac
      481 gcatgctgtt tcctttctta ttactactaa ttaattatac cttgaatatt cttgcttgca
      541 aatacatcat catagttatt ccttgactta attaacagtt cacgttacgg ttaaatagta
      601 aagtgaattg gatttctcat acacagcgta atgacctggt ggagaaaagt tgaatcctga
      661 ctttctaagg ggagatgcgc gcatctctcc ttttgcgctt ttttaagcgc cctgccttcg
      721 atgatgtgca cgagattagc ctttagaatt cacttaaata aaatctgtcc gttaccaaag
      781 cagacagtca cattttaagg aggaattttg gttatgtcag tatcattaga agtaagtaaa
      841 agagaagtac gtccccgttc attaagaaac aaacttcgtc acgaagggaa agttccagct
      901 gttgtttatg ggtataaagt agaaagtacc cctatctcat tcgatggttt gacactaagc
      961 aaattgttgc gtgaacatgg agcgaacaca gtcatcacga tggacatcga tggacaaaaa
     1021 gtcaatacat tggttcacaa gatccaaacg aacactttta ccaatcattt cgaacatgca
     1081 gaatttctat ctgtaaacat gtcggaagaa acagaagtcg aaacagaagt cgttcttgtt
     1141 ggcgaatcag tgggtgctaa atctggtgga gtgttagctc aaaacttgta tacagtagtc
     1201 gtatctgcta caccagacaa attgcctgaa cgaattgaag ttgacgtgac aaacttggaa
     1261 ttaggtgatt ctattacagt agccgatctt ccaaaaaacg acgattacaa attcgtgacc
     1321 gacagtgaag agcaaatcgc tgctgtagtc gctcaaagtg aagaagaaag cacaggtgaa
     1381 ggtggcgaac cagaagtgat cggttcaaca gaagaataat actgaaacaa tcaggtggtg
     1441 ttagaagcgg cgggtcagca gcttttaaca cgcctttttt atttctaatc gtttattttc
     1501 atatggggct tccacttttg taaatggctt tacaaacgaa aaatcctaat ttaccaagct
     1561 tttcctcgca tacttttatc gtacctttat aaaaatttat actttgaaaa aaagtattca
     1621 attattgttg catttttcct gattctttat tagaatgaac cctcgttgaa gtaagtattt
     1681 ctagtttatc ctaggattaa ttataaaaat aaaacattcg ttgattcgaa ggaggttaaa
     1741 cagatgtttg aaagttttga tagcagccgc aaccgttacg cttcagttgg tgtcgtatca
     1801 agtttgccag atgaattgat tgacagtata tggtttatta ttgacctaga tcttaaggga
     1861 gtcattccat tagaaaatat attaaccttt gatttgatcg aaaatcgtgg aaaagtgacg
     1921 atgcattttt ctcaagcagg aagcaatgtg gaaatggata ttgatttgcc gtttgcctat
     1981 tcgaaccaat ttcctgctca agtatacgcc tatgatgacg gtacgagaga aacgatcctt
     2041 ttaccaagtg agattagaca atattaaggt gatagataaa cgtggatgtc ccttatttta
     2101 cagaccaggc caagttgttg attctttgac tcaattcttg gcttcttttt atggagtttc
     2161 ataaattatc attgatttct aattggtatt ctcaggataa acgctatata ctactaacta
     2221 aattttaaag atataaggag tttaaaagat gcgcgattta acaaaaggaa gccccgctaa
     2281 actgattttg atgttcactg ttcctttact gatcggaaat gtttttcagc agttctacaa
     2341 tatgatcgat atgatcatcg tgggtcagac gctaggtaaa aatgcgctgg cagcggttgg
     2401 tgcaactgga agcctgacat ttttgatcat tggctttgcc caaggtttaa cagctggttt
     2461 agccattatt accgcacaac gttacggtgc aaaagattat cggggtctga agaaaagttt
     2521 tgctgctagt gttgtcatta gtcttgtcgt cactgtgatt ttaactgtcc taagtctcct
     2581 gtttattcgt ccgatgcttc aattgatgca aactcctcct gaaatacttg atcaagcaca
     2641 aacatttatt tcaattattt tattagggat ctttgcatca gtcagtttca atttgctttc
     2701 taatgtcatt cgcgcacttg gtgacagccg cacaccacta ttctttctga tcattgcggt
     2761 catcatcaac gtcgtacttg atttgatctt tattatctat tttggcatgg gtgtggaagg
     2821 tgcagctatt gctacagtaa tcgcacaagt ttcttccagc gtactgtgtc tagtgtatat
     2881 caagaaaaaa atgcctttgt tacagttacg caaaaaagat ttcaaatttg aaaaagaaga
     2941 gtttgctgta cacctaaatg ctgctttgcc aatggcgttt caatcttcga tcatcgctgt
     3001 tggtgctatc gtgttacaag cagcactaaa tagtctaggg acagatgtcg tagctgctca
     3061 agccgctgct ggtcgtatcg accaatttgc aaatcagccg atgatgtcat ttggtattgc
     3121 aatggcaact ttcactgcac aaaactatgg tgcgaaagaa tatggccgaa tcttgcaagg
     3181 ggtacgtcaa actttactga tgagtgtcgg atttagtctc ttagcaggtg ctatcgtcat
     3241 tttcttcggt catttctttg ttcaattgtt tgttagccca tcagaaacac gtgtatttga
     3301 attagcacaa acctacttca atatcaatgg gtctctttac tggatcttag cagtcctgtt
     3361 cattttacgc tacacacttc aagggcttgg acaaagcaag atccctacga tcgccggtat
     3421 gatggaattg cttatgcgtt cttttgcagc tatcgttctt acaggaatgt ggggataccc
     3481 aggagcagca gcagcttctc ctttagcttg gatcggttct gttgccgtgt tgctttactc
     3541 ttatatgcgt tcgatgaaac aattgaaaca catgggtgaa gaggaacatt tcacattaga
     3601 acatgcagaa gcaagatagc cttgattaaa agaaatcgtt gcggctaaaa atgaaaactt
     3661 ttattgaaaa tatcttcttc gtttaccgaa aacaggtttt ttagataaca aaaaggctgt
     3721 gacaaaaaat gtcacagcct tttttctttt attctatttt tctttccgtg gactctactt
     3781 tgaaataatc gatatgtgca tgctggtatt ctaataccgt gaaacggaat gatgcaatct
     3841 caatgattgt tcctgctttt atttctttat atcggctaga agcaaagccc gcaagtgtag
     3901 taaaattcgt ttcttcaaac tctggaatct ctacatgaaa ataacgctgg aaatcatcta
     3961 atgttagttt gccggagatg atataagagc catctgactg ttccgctatc aatgcttcgt
     4021 ccaaatctgt ctcatctcgg atttccccga cgatttcttc taaaatatct tcaatcgtca
     4081 ctaagccgct tgtccctccg tattcatctt ttaagatagc aatatgcttt cccttttttc
     4141 gcatgatcgt caataatttt tttataggga tcgtttcagt tgttacgatc ggctcttcaa
     4201 tcaactgttc tactgtcagc tggtcatctt tataagattg cttgatcaaa tctggcaagg
     4261 tgacataacc taaaacattg tcttttgatt ctaagataac cggatagcga gtatgtcctt
     4321 gttcaatcga agcttttatc gcttctttga cggtttctga agcttcgaat acttgcatgg
     4381 acgtacggtc tacttgtata tcttttgcca aggtatcatc aaattcaaac acgttttcca
     4441 aataatgata ttcttctttg gtcaactctc ctttttcata agaattcaat gcgactaatg
     4501 tcagctcttc ttgagacaat gtctcttctc cttcagatac gagacgcaca cctaatagct
     4561 tccctagccc tgcagctgaa tgattcaata cccaaataaa aggaaacatt actttataaa
     4621 aataatgaag tggtttaact acgaacaaga cgaccttctc cgtattgacg atactatagc
     4681 tcttaggaag cagttcgcca ataaccacat cgatatacgt aactaaaata aatcccagcg
     4741 ccacagagat tgtactgcta atagccgaac taaaaggtag tttaccgatc actggttcta
     4801 gtaaagccgc gacagatcct tcaccgaccc acccgatgat caaaccggtt agcgtaattc
     4861 ctaattgact agctgataaa tagttatcta aatggtgtgt aacatgaaga gctagcttgg
     4921 cgttctttac attttccttt tctagttgtt ccaatcttga tcgtctgatt ttcactaaag
     4981 caaattcact tgctacaaaa aatgaagtaa ttgcaatcat caaaaataaa ataatgaaat
     5041 tgataaccat attctcccgc ctttctcttt ttctattgta ccctttttct acaaaaagat
     5101 tgaaaataat gcgtattcat atttaggaat aaaaaaggag atgcggcatt tttcagccac
     5161 atctccttat cacttcaaaa tcattgcgtt acttgttgct aaacagcttt ttcatgacct
     5221 ctgataaacg gtgttcaagt agctgacatt cgacattagt tcaggtcaaa gcgctacttt
     5281 cacaaccgct ctttcaacca tgttttccga gtatttccaa tgataagcgg aacagtttca
     5341 tgttttactt cgctgtactc tagcccaaac catgtttctg gcgaggtagc gatttttttg
     5401 attgccagtt ttgcctgcat gatttgacgt tgccattttc ctagagttgt cacattggaa
     5461 actacttcat caatcaggac aaattggaaa tcgccaactt ctcgccctgg tgtcagagag
     5521 tatctttgtg gctgttttgg caaacggcct tgtttcatca aatcatggat gacttggcga
     5581 acatatacgt tgatttcctg ttggaccctg aaacctaaga acagattcac ttggacaata
     5641 aaatcagtgt ccatcatatc tacttgatat tctttcgtat atggctcatc cgtcacttcc
     5701 acatgaacaa accaatagac tcgtgctctt ttaggccgtt tatctaaaat cgaatagatg
     5761 aactgtcggc caactttatc ttcagccata tcaggaacca agaagaccac atttgtctga
     5821 tagattggta aagaagtatc atcacgtaat tctctaagct gcgggatata atctctcaaa
     5881 gacacatcct cggttgacga ttcgcgaata atgttccctt gttcccaaat aaacatcacg
     5941 actaagatga tcaaaccaat cagaacggct acaaaaccgc ctcggaagaa tttgacaaca
     6001 cttgagataa agaaaacgac ttctatcgca ccgaagaaaa aggtaactaa atatgcaagc
     6061 caacgagaat gcccattttg gataagataa aagtaaagca gtgctgttgt catcaacatc
     6121 gtcacagtga tcgatagacc gtaagcagct tccatatgat gagacgttcg gaatgtgaca
     6181 accactagag aacaggcgat ccataaaatc agattgactg ctgggatata catttgcccg
     6241 atactgttcc caggatacat gatctttaac cgaggcagca gtttcaactt gattgcttct
     6301 gaaactagcg taaacgaacc tgtaatcaat gcttgcgaag caatgactgc agcgactgtt
     6361 gcaaaagcaa cgccgatcac gatccaactt tgaggcatca tttggaagaa tggattcagg
     6421 ttcttgattt gctgtgcttc agggttttga taaacattca acaaccacgc agcctgccca
     6481 aaataattga taaccaaaca aattttgacg taaggccagc tcagacgaat atttcgtttg
     6541 ccgacatgtc caagatcgga ataaagcgcc tctgcacctg tcgtagccag gaaaatattt
     6601 ccaagaatga acaagcctaa tttgttatct gcactaaaaa gcagctggat agcataataa
     6661 ggattcagtg cacggataac tgtccagtct tgactgaagt tcattaatcc cattacgcct
     6721 aaaaaagtaa accaagcgaa catgattggt ccgaatgctt ttccgacaat atctgtacca
     6781 aagcgttgga ccatgaataa aacaaaaata ataactaaag tgataatgac gatcacgttc
     6841 tggttattac cgaaccggtc aaaaaactgc ggaattcctc gcaagccttc aatcgcagtc
     6901 gttaccgtca cagcaggggt caatacccca tctgctagta aggcagctcc tccgatcatt
     6961 gcaggaatga tcaaatattt cccacctttt cggacaagtg tgtagagtga gaaaattcca
     7021 ccttcaccat ggttatctgc attcaatgca atcacgacat atttgattgt tgtcagtaga
     7081 gtcaatgtcc aaaaaatcaa tgagactgat ccgataacaa aatcaggatt gattcctgac
     7141 aatccgccat tatcttcaac aatcgatttc ataacgtaaa gcggacttgt cccgatgtct
     7201 ccgtagacga ctcccatcgc tacaagcagg ccagccattg atatcttttt ggtattgtgc
     7261 gttgttgcca cgtttttccc tcatttcttg ttatatggat atcctttagg taagcttgaa
     7321 tgatagatga tattcttatc catgacaccc gctttcctaa attgcttcta attatccaag
     7381 taacacactg aagtgtcaac ttatttgaat aaaattattc tttctttgga cgttctctga
     7441 tacgtcttgc cacaaatgaa taaagagcaa ggcaaaaaat tgcacccaat aatgctggta
     7501 tcaaataaaa accgccgagt tccggtccca gtggtcctag taaaaattcg ccgagccaac
     7561 tacctaaaaa accgataatg atatttccag atacgcctcc cgggacttcc tttcctaaaa
     7621 ttgcgccagc tatcaaacct aatataccgc cgataaataa cgaccataaa aaattcagca
     7681 tctcctttcc ctcctttatc catatttctc acttttattt tactctttat tcagaaggac
     7741 tccaaaaata tgcgccaaga aactccagaa aaaaagaagt tgtaacaaaa gtgttcagct
     7801 tatttccgta gaagctgctt ctggaacacc gtgaatcaga ggttgtgaaa tcagcggttt
     7861 gacctgagta ttagagaaac aaacggaaaa atagttcctc atattttgcc gttttttgag
     7921 ctaatatcga gggacagctg attgaacatc gtttattgga gaggttgtaa aaaagctgtt
     7981 ctgcatcaag taataagaac agccaagtaa aaacagctcc taatgttttt aacttggctg
     8041 gacttattac cgcagatgca agcttttgaa caccgtttat tcgaaaaaaa aggactgtga
     8101 cacggctttt gtcacagtcc ccttttttcg aataattatt gtccccctgt caccttcacg
     8161 atcacaagta cattattcct atgaattaaa ttatttttct tcatttttcg ttgaaccatc
     8221 ttcgttcaac attgcttttc ttgacagatt tacgcgtcct tgtttatcga tttcagttac
     8281 tttcacaagg atctcatcac ctagtttaac aacatcttcg actttattga cacgatcgtt
     8341 tgctaattgg gaaatatgga caagtccatc ttttcctttg atcaagttga cgaatgcacc
     8401 gaatttttcg atacggacaa ctttccctag ataaacttca ccaactttga cttctttggt
     8461 caaatcttcg atgatcttga tagctttttt gatcatttct gcatcagctg aagcaatact
     8521 tacttttcca tcttgatcga tatcgatttt cacaccagtt tcatcgatga ttccgttgat
     8581 cgtatcgccg cctttaccga taacatcttt gattttagca ggttcgatct ggatcatttc
     8641 gatttttggt gcatattggc ttaattcttc acgtggagca gcaatcgttg aagtcaattc
     8701 ttctaagatt tccatacgtg cttgttttgc ttgagttaat gcttcagtta ggatttgttc
     8761 tgtgattcct tgaatcttga tatccatctg taaagcagtg ataccatctt ttgtaccggc
     8821 aactttaaag tccatatcgc caagatgatc ttccagccct tggatatcag ttaggatcgt
     8881 atagttttca ccatcagaaa caagtcccat cgcaataccg gcgactggcg ctttgattgg
     8941 tacacctgca tccatcaatg ctaatgtgcc agcacagata cttgcttggg aagaagaacc
     9001 atttgattcc aatacttctg ctactaaacg gatcgtataa ggaaattctt cttctgaagg
     9061 gattacttgt gctaacgcac gttcacctaa tgcgccatgt ccgatttccc gacgccctgg
     9121 agagcctgca cgtcctgtag aaccaacaga aaattgaggg aaattgtagt ggtggatgaa
     9181 tcgtttgctg acttccaccc ctaaaccatc aatgatttgg tgctctccta gaggtgcgag
     9241 tgtacaagct gataaagctt gtgtttgtcc tcgtgtgaac aaacctgatc catgtacacg
     9301 tggcaataag ctgacctctg atgacaatgg acggatttca tccaatttac gcccgtcagg
     9361 acggatctta tcgatggtga tcaattcacg tacgacatct ttttcaagat cttctgtgat
     9421 ttgtttaact tctttcaaca agtgatcttc atcttcatga cctgcaaatt tctcagcata
     9481 agcttcgcgg atatcgatct tcacctgctc gatattatct tcgcgagcaa gtttttcttc
     9541 agtcattact gctgctttca ttgtagcgta agaagcatca aagatttctt ttttcaatac
     9601 ttcatcaact tgcagtaaag tcacttccat tttttctttc cctactgctt gaacgatttc
     9661 ttcttggaaa gcaactaact ctttgatagc gtcaaaacca aacagcaatg caccaagcat
     9721 gtcttcttct gaaacttctt tcgcgccgct ttcaaccata ttgatggctt gtttcgttcc
     9781 ggcaacagtc aattcgatat ctgtttgttc tgcttgttct actgttggat tcaagacgta
     9841 ctcgccattt acacgaccaa cttctacgcc tgcgattggg ccgtcaaatg ggatatctga
     9901 gattgatagt gctaaagaag aacctagcat tgctgccata gctggcgagc agtctgtttc
     9961 tacactcatc acgatgttcg ttacttggac ttcattacgg aagccttctg caaacatcgg
    10021 gcgaattgga cggtcgatca aacgggcagt caatgttgct tccgtacttg gacggccttc
    10081 tcgtttgatg aaacctccag ggattttccc tgctgcatac attttttctt cataattgat
    10141 cgttaatggg aaaaagtctg tatcttttgc ttctttagag gccacagctg cacttaagac
    10201 tactgtatct ccataacgga ctaaaacggc tccgtttgct tgttttgcta gctgaccgac
    10261 ttcgatttgt aagggacggc cgccccatgt cgttttgaat acttgttttt ctgacataac
    10321 ttctcctttt ccttctcgtg aaagcaagac gctactaaac aaatgaaaac tgccgaaagc
    10381 cgtcttttga gcagctttca tttggttagt aggagtccat ctagcttgat cacggaattt
    10441 tacttaattg atatacgagt aaaaaaagtg agattcatgt gaatcccact ctttttgact
    10501 aaaattaacg acgcaagcct aaacgtttga tcaattcgcg gtaacgttga acgtcagttt
    10561 tacgtaagta agctaacaag ttacggcggt gaccaacttt tttcattagg ccgcggtatg
    10621 aatgatggtc ttttttgtgc acgcgtgcat gttcattcaa gtggttgatt tcttcagtta
    10681 atacagcgat ttgtacttct ggagaaccag tatccccttc atggcgagcg tattcgttga
    10741 tgatttcatt cttgcgttct tttgaaattg ccatttcttt cacctctttc gattattccc
    10801 ctgcgactga gtaaaacgtt ggtgattcgc ttaaccaagt ggcaggtcat gtgcctagag
    10861 cacacagcta tactttacct gaaaccgggt tgaaaatcaa gtagttaatc attaagaaaa
    10921 ggtagttttc aaacaatctt actactactt ttccattttt gtggtaaact ataatgcgaa
    10981 aatagacttg aaacacagtc ttttaagaaa ggggtacata ttccatgatt acaatggatg
    11041 atatcattcg tgaaggaaat ccgaccttac gcgaagttgc aaaagaagtt tctcttccat
    11101 taagcgaaga agatatctct ttaggaaaag agatgcttga gttcttaaaa aacagccaag
    11161 atccaatcaa agctgaagaa ctacacttac gaggcggtgt tggcttagct gctcctcaat
    11221 tagatatttc taaaagaatc attgctgttc acgtaccaag tcctgaccca gaggcagatg
    11281 gaccttctct aagtactgtt atgtataatc ctaaaatttt gagccattct gttcaagatg
    11341 cttgtttagg ggaaggtgaa ggctgtttat ctgttgatcg agaagtacca ggatatgttg
    11401 ttcgccatgc caaaatcact gtgtcttatt atgatatgaa tggtgaaaaa cacaagattc
    11461 gtttgaagaa ttatgaatcg atcgtcgttc aacatgaaat cgaccatatc aacggtgtga
    11521 tgttctatga ccacatcaac gatcaaaatc catttgcact aaaagaaggc gtattggtga
    11581 tcgagtagag aattggcagg ttccttcgat actatgctac aagaaagctt tataaaaaag
    11641 gggagtgctc aaaagctgca cttccctttt ttatgtttct ccactaatcg aaatttagat
    11701 agacatctag aaatcgcaat tccaaaaaat tctgtgtatt ggatacgcat cttttcttat
    11761 tttttcttat ttcttccata tgggtgttta tctcagagat gatagaatag tttctaggat
    11821 aaaaagtagc agctacgatg ttatccttcg tagaggattc ctgcttcagc tagtttttgt
    11881 ttgatgtttt tcagcatatg cttttctggg tagcggatcg tatgcagatg gatgccgcct
    11941 gttaattcgg ataacaaagt agcattactt ttttgataac gtctcataaa ggaatcgata
    12001 tcttgtggcg ttttgatgtg caaagttcct gctaactcac cgtaaagggg atgttccaca
    12061 attacatcaa tgatttcacc accattttca acgattagtc tcagttcttc ttctgtttga
    12121 tcttttccat gctggacagc gattttactg atgtgcccag tattggtttc ttctgtttcg
    12181 aggatataac ctcttgctgt tgccacgata cttttacctg cagctcgtag taaagcaata
    12241 tcgcctacaa tgatctgacg gctgactcca aaagttttcg caaacttgct agcactcact
    12301 ggtttttctg tatttgccaa ggtttgcatg atttgttttc gccgtgtctc tccttcgatc
    12361 acacttattc ctccttcatt ttgttttggt attcgcgcga gtgtttcatc aaatcaacat
    12421 acgtgtctgt tattgtttcc ttatatccct tatttttcag cagactaaca actttatcca
    12481 atacttgctg ctctcttttt gcatcataaa ctgcttggtt ttcttctttt ttcacttgtc
    12541 ctattttagt taccgtatcc attctttttt ctaataaagc aaccaactct cgatcgatca
    12601 tatcgatctt atgacgtaat gtttcaagag actcttctgt tagttcttcc tctactgtct
    12661 tgatgatacg agcaggattt cctgcaatca ctacattgtc gggaaaagat ttggtcacaa
    12721 ccgaacctgc gccgactact acgttgtccc ctagagtgac acccggcgtg acgataactc
    12781 ctccgcctaa ccaaacatta ttaccgattt taatgggttt tgcgtactcc agaccgctgt
    12841 tacgtttcac tggatgtagt ggatgcgtcg ccgtgtaaag ctggacattc ggagcgaaca
    12901 tgcagttatc accgatttca atcgtgctga catctaaaaa tgtacaattg aaatttgcat
    12961 aaaaattctc cccaacaaat atattatatc cgtaatcaaa attgataact ggctccatat
    13021 agattttttt ccctgtacgg ccaaaagtct ctttcaacaa ttgggatcgc agttccgctg
    13081 attctgcttg gttgatgatt tggctttgac ttcttgcaaa catacggtcg gcaaccagct
    13141 caggatcatt ggcaaaataa agctcgcctg caatcatttt ttctttttcg gttttgaatt
    13201 cagtcataat ctgctcctaa ttcttgtatt tttttgaaaa caaccgctcc gtcttcttga
    13261 ataaacgtat aaggctccgc taaataaaca ggcgtttgat tgattactaa gattttggct
    13321 gacggctggc gaaactgtac cagatcacaa aacggatgaa cttgaaaact cgttccaaca
    13381 atgacgatca gctctgcttg gctgacggca taagccgcct gctccatgac tccatcttga
    13441 aacccttcac catataacac gatgtctggg cgtatttgtc ctccgcatgc ttgatgtttg
    13501 tcgctgtgaa gatattcttt ccagtctacc gtttgttgac attttcgaca atgacattga
    13561 tacagactcc catgaaaatt gacgagttgc gggcttttag cctttgcgtg caaaccgtcg
    13621 atgttttggg aaacaaccca aacatttttt tctttcgcta gatgtgctat ttccaaatga
    13681 atgatattgg gttttgcttc aggatgataa atctttttga caaattgata gaatttatcc
    13741 ggttcattca ccattgcttg atgactcagc aagtattccg gctgctccat cccgtggtag
    13801 attcctgaca aagatcgata gtctggcact ccagaaggtg tggatacccc tgcaccggtc
    13861 aaaaatgtaa tttgctgata agctaatatc tcttttgctg cttcgtctac tgttaattgt
    13921 ttcatgtttt gtcacctcac tttccattat agcccatact cgaacaaatt tattgttgaa
    13981 aagtcagaaa aatgggaata aaaaagtgtt atgggcattt gagcaattta tcacaatgtt
    14041 cactgacaaa agaatgaaaa cgtgttacaa tactgctgta taaaagtgag gtgaatcgat
    14101 atgttaccat tctttattta ttggggcgtt attctggctt gtatcgcctg gttagcgtta
    14161 agcgtttatt tctctgtttt ttatcttgta cgcaaagaaa atggaaatct ttgggctttt
    14221 gctttattca acgtgattgc cgcaattgtt ttagccatta cattagccgt atatcgaaca
    14281 tggggctggg gaatcaccca atactcaagt ttgatttatc taatattagc catttatggc
    14341 gtcgttgttc ttttgcaagc gatcctagga cgtgagccaa agaaagcggc agcttaaatg
    14401 atcgagatgg aactggacac aggaaatttt ctgttctagt tcttttttta ttgtattaca
    14461 tacatttttg tgtataatta tactatcgtt tgcaatggat agatgattca cattttgatt
    14521 gttttttcgg taaaagtaaa gatttctata tttatcagta ttttttcaaa aaccagcagt
    14581 tatagattga caaagcctct cgagtatcgt aaactttttt tgtataaata actgaatacg
    14641 agatgcctca atcaatgagg tagaggtcgc gagaatcatt aaccctgtgg agtgaagcaa
    14701 acatggagaa gcaggaatag ggaggatcgc cgaaatgtgc acttttttgc ttggagtgta
    14761 tgttgggacg caagttaaga gcttgcggac tgtcttagca gtgatgctaa gttgcgctat
    14821 gttttgtgag aggtttatca ccttatggaa ttttagcggc ctttttatat ctataaatcg
    14881 gtcgcttttt attttttcag ataaagcttt accgcatttc atctagaccg ctcgtgtatc
    14941 atataatcag aaagggagaa aaaaatgaaa aaaaatatgg tttcagttgc cgtactattt
    15001 gcttttgtct tctcactatt cagtgtggga tctctggcaa atgcagatga aaaaacaccg
    15061 agcgatgagt ttcgcgtagg tatggaggct gggtatgcac ccttcaactg gtctcaacaa
    15121 acagatgaga ataatgcctt gccaattcaa ggccaaaata gttacgccgg cggttatgat
    15181 gttcaaatcg ctaaaaaagt agcagatggt ttagggaaaa aattagtcat cgtccaaacg
    15241 aaatgggatg gtctggctcc tgcccttcaa tcaggcaaga tcgacgcgat catcgccgga
    15301 atgagcccta ctgctgaacg acgaaaagaa attgatttta ccgatcctta ttacgaatca
    15361 caattagtag tagtagttca aaaagatagc aaatatgcaa aagcaaaaag tttagcagat
    15421 ctttctggag caaagatcac tgctcagtta aatacattcc actatactgt gatcgatcag
    15481 ataccagatg tgcagaaaca acaagcaatg gataatttct cagcaatgag aacagcttta
    15541 gcttctggaa tgatcgatgg ttatgtcagc gaacgtccgg aaggtgtaac ggcaactagt
    15601 gtgaacaaag acttggaaat gcttgaattc agcggtgcag atggcttcca gacaaacccg
    15661 gaagatgttc agatttctgt tggtatgcgt aaaggcgacc cggatatcca aaaagtaaac
    15721 cagattcttt ctggcatctc taacgatgaa cgaaccaaaa ttatggacca agcagtcaaa
    15781 gatcagcctg ctgcaactga tagtgaagaa aaacctggca tcatttctga tttcactacg
    15841 atttggaaac aatacggcaa tatgttctta cgaggtgctg gacttacctt gttcatcgca
    15901 ttgatcggga cagttgtcgg tactacactt ggattactga ttggtgtctt ccgttcggta
    15961 cctgaatcag gaaatgcact gactcgcttc ttccaaaaaa tcggaaatgc acttctttct
    16021 atctatgtcg aagtattccg cggaacaccg atgatggtac aagcaatggt catcttctac
    16081 ggtctggcac tcgcttttgg catttcattg aaccgaacga ttgccgcttt gtttattgtc
    16141 tctctcaata ctggagctta catgacagag atcgttcgtg gggggatttt tgcagtagat
    16201 gaagggcagt tcgaagctgc gcaagccatt ggtatgaccc atggccaaac gatgagaaaa
    16261 gttgttattc cacaagtttt acggaatatc ttgccagcaa caggaaatga gtttgttatc
    16321 aatatcaaag atacagcagt tttaagtgta atcggcgtag ctgatttgtt cttccaagga
    16381 aactctgctt ccggtgcgaa tttccaattc ttccaaacat ttacgatcgt cgggatcatc
    16441 tacttagtca tgacctttac gatcaccaga atcctgcggg cggtagaaaa gaaaatcgat
    16501 ggtccttcag cttataccaa gcttgaagaa atcgacaatt caaatttgca ggaataagga
    16561 ggaagaatca tgaatgaaaa aattattgaa atcaaacatc taagaaaaag cttcggtgaa
    16621 aatgaagtat tgaaagatat cagcgtaaca gtcaataaag gtgaagttgt gacgatcatc
    16681 ggttcttccg gttctggtaa atctactttc ttgcgctgta tcaatttact ggaaaaacca
    16741 acaggcggcg agatcattta taatggagaa aatgtattag ctcctaaata taatttacct
    16801 aaatatcgaa cgaatctagg tatggttttc caatccttca acctgttcaa caatatgaat
    16861 gtcttggaaa actgtatgtc tggtcaaaca actgtcctga aacgagataa agaaactgcc
    16921 aaaaaagtag caatcgaaaa tctggaaaaa gttggtatgg atcgttatat cgaagccaaa
    16981 ccagcacaat tatctggtgg acagaaacag cgtgtcgcaa ttgctcgggc attatcaatg
    17041 aatccagatg tattgctatt cgacgaacct acttcggcgt tagaccctga aatggttggc
    17101 gaagtgctta atacgatcaa agacttagca catactggct tgacaatgct tatcgtgacg
    17161 catgagatgg aatttgcaaa agatgtatct gaccgtgtca tttttatgga taaaggtgtg
    17221 attgcagaag aaggtacacc agaagatatc tttgtccatc cgaaagaaga acgtacaaaa
    17281 gaattccttt cacgtatttt aaatgcttag gatcaatagc tgattgggac aaagagctgc
    17341 ggtgtttacc gcagttcttt tt
//
LOCUS       DAZKCY010000053        16885 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000053 DAZKCY010000000
VERSION     DAZKCY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.053"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(259..1764)
                     /locus_tag="ACG8XC_001600"
     CDS             complement(259..1764)
                     /locus_tag="ACG8XC_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9191296.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1761..2447)
                     /locus_tag="ACG8XC_001601"
     CDS             complement(1761..2447)
                     /locus_tag="ACG8XC_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9191297.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2640..4061)
                     /gene="gndA"
                     /locus_tag="ACG8XC_001602"
     CDS             complement(2640..4061)
                     /gene="gndA"
                     /locus_tag="ACG8XC_001602"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP9191298.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4231..4410)
                     /gene="rpmF"
                     /locus_tag="ACG8XC_001603"
     CDS             complement(4231..4410)
                     /gene="rpmF"
                     /locus_tag="ACG8XC_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9191299.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4468..5037)
                     /locus_tag="ACG8XC_001604"
     CDS             complement(4468..5037)
                     /locus_tag="ACG8XC_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFP9191300.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5223..6125
                     /locus_tag="ACG8XC_001605"
     CDS             5223..6125
                     /locus_tag="ACG8XC_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9191301.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6160..7944)
                     /gene="pyk"
                     /locus_tag="ACG8XC_001606"
     CDS             complement(6160..7944)
                     /gene="pyk"
                     /locus_tag="ACG8XC_001606"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP9191302.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8018..8980)
                     /gene="pfkA"
                     /locus_tag="ACG8XC_001607"
     CDS             complement(8018..8980)
                     /gene="pfkA"
                     /locus_tag="ACG8XC_001607"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP9191303.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9148..12462)
                     /gene="dnaE"
                     /locus_tag="ACG8XC_001608"
     CDS             complement(9148..12462)
                     /gene="dnaE"
                     /locus_tag="ACG8XC_001608"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP9191304.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12693..12878
                     /locus_tag="ACG8XC_001609"
     CDS             12693..12878
                     /locus_tag="ACG8XC_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFP9191305.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12940..14136)
                     /locus_tag="ACG8XC_001610"
     CDS             complement(12940..14136)
                     /locus_tag="ACG8XC_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP9191306.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14306..15742)
                     /locus_tag="ACG8XC_001611"
     CDS             complement(14306..15742)
                     /locus_tag="ACG8XC_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP9191307.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16035..16844
                     /locus_tag="ACG8XC_001612"
     CDS             16035..16844
                     /locus_tag="ACG8XC_001612"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9191308.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4955 a         3540 c         2949 g         5441 t
ORIGIN      
        1 caaataataa tatagcaatc tctttataaa attgcaatag aaaattccct atttttattt
       61 attattttta aacacgaata ttacagcatt ttattgtttt ttcgttcatc taaaagatac
      121 ttacatttaa ttaacggatg ttattctatt taattgttcg ctagtttttt tataatgaca
      181 tccctgaatt tctcacttgc ttgtattttg taccttattc tttgttggat atataaaaaa
      241 acaagtaaag cacatgcttt acttgtcttc tgcctttgcg ataggaataa agattctaaa
      301 aattgtccct tgccctacta cactttctgc taagatacgt cctttatagt tttcaaccag
      361 ttgtttagcg atagacaacc ccagaccatt tccacccttc gttctagctc ttgctttatc
      421 cactcgataa aaacgattga agatcttatt aaggtcttct ttgggtattc cttcgccaaa
      481 gtcctgaatc gcgatctcaa attcattcag agtggaagaa atagagatat ggacttcttt
      541 tcgtgtagtt gaatacttta ctgcgttatc caatatgata atgattaatt gttcaaagtg
      601 attacgataa atttgcaggg tcacttcttt tgtcaaatca tcatctaaag tgatcacata
      661 gtctggatat aatatcttga aattattata aacttgatag gtgacttctt ttcctttagt
      721 tgtttcattc gcataatgaa catctacttg ttctgctctt gataagtcta gcatctcttg
      781 aactaagctt ttcatacggc tgatttcttg catgctggcg cttaatgatt cttctaatat
      841 ctcaggatca tctttccccc aacgatttag cagactaaga tgcccttcaa taattgcaac
      901 aggtgtcctt aactcatgag atacgtcttc cacgaattgc tcttgttgtt caatatacaa
      961 acgcatacgg tcaagcatct cattgaatat ttcagctaaa tctgccaatt catcatttgt
     1021 gtcgatttca ggcatgtgga tatctgattg cggatcttta cgtatagtat ccattgtatc
     1081 tctgagtacc ttgagtggtt ttaagaaata agaagataaa aagaaaccta aaatactact
     1141 taagatcaaa gaaacaactt ctaaaacaat aagagtcaat aatagcttat ttcgaatatc
     1201 atagaaagaa gataattcat agaagctttg cgcatagccg atcttttctc ttgtaccttt
     1261 agaatatata ggctggatag ataaaaagcc agtttttcct tctagtgtaa cgatcgttgg
     1321 aactttacgt gtagtctgaa tcaggtttcg actgttttca tgggttttaa aaatcaagtt
     1381 ctcgtccaaa tcataaacat aaagatccat tgatggctgc cctagctcag ctataaagga
     1441 atcgatcttc attaagtttc cttcgaccag tgaagtatga tctacgacct catctccgtc
     1501 atttgtcgct ttcgtcagat tacgatacgt atttactaat gtcagttctt catccgagtt
     1561 agccaaacga gaagctactt ctgaaatcgt acgctctaca ttgtttcttt cttttgccac
     1621 aatcagattg atagatgatt tataggtaat gacagcgaaa attgtaaaca caacgaatat
     1681 gaagaaagaa ctcgcaaagg cccacttgat tgtcaaggag ggccctttca gttctctttt
     1741 ttccgatttt ttcggcttta tcacgagcgc atcacataac ctgttccgcg tacagtttgg
     1801 atatagcttt cctccccggg aacgtcaatt ttgttgcgaa gataacgaat atatacgtcc
     1861 actacgtttg tttcaacttc tgtttcgtat ccccacactt tatttagtaa aacgtcacgt
     1921 gctaatacca cgttcacgtt ttccattaaa gtaagcagca actcgtattc tctttttgtt
     1981 agttcaatta cttcatttcc gcgacgtacg acacggtttt ctttttcgat agtcaaatcg
     2041 cgataagtaa tagtagtttg tttagccaca tttttatcgc cttcaatatc gattcgacgc
     2101 aacaatgcac gaagacgtgc taataattct tcgatagcaa atggtttgac gatgtagtca
     2161 tctgctccgt ggtcaagtcc agatacacga tcgatcacag aatctcttgc agtcatcata
     2221 atgattggtg tatttttgac ttggcgtaca cgtctgcaga cttctaaacc gttcaattct
     2281 gggagcatca gatccaacaa tatggcatcc cactcattat ttaatgccgc atctaaacca
     2341 gttcggccgt tgtagtggac ttctgcgtta tacccttcat gcttcaactc tagctcgaca
     2401 aatcgtgcca agttcttttc atcttcaatg attagaatat tgctcattta ttattttatc
     2461 ctttctattc tactggcttt ttaaaaactc tatcattata ccgataatat gcttaaaaat
     2521 ctatagtcat tttattatgg atagcaattt taaaaaagag ctgaagtcta ctcataacaa
     2581 tcgacttcag ctctttcgtc ctctttttat tttctcattt cctaacagcc gaaagactat
     2641 tattcttcat ggtaccatga gtaatggaag attccttctt tgtcgacacg ctcataggta
     2701 tgtgcgccaa agtaatcgcg ttgggcttgg attatatttg caggcagacg ttctgctctg
     2761 tatgaatcga agtaagcgat ggctgatgag aaagtcggta caggtacgcc agcttggaca
     2821 gccaaggcaa cgacgtctct gactgattgc tgatattttt tcgtgatttc aacgaagtaa
     2881 tcatctagca ataaattttc gatctcagga tttttgtcat aggcatccgt aattttttgc
     2941 aagaatcgag ctcggatgat acatccagcg cgccagattt ttgcgatttc accaaatggc
     3001 aagtcccaat caaattcttt tgaagcgaca cgtaattgag caaatccttg ggcataactc
     3061 atgattttac tgaaatacaa cgcttctcgg attttttcga ccaattcttt tttgtctccc
     3121 gtgaactcaa aatcgttcgt acgagacaaa atcttgcttg cttgtacgcg ttcttcttta
     3181 taagcagaaa tatagcgggc aaatacagat tcagtgatca atggaagtgg cacacctaag
     3241 tcaagcgcgc tttggcttgt ccatttacct gttcctttat tcccagctgc atcaaggatc
     3301 acatctacta ctggtttacc tgttccttca tcgtctttac gtgtcagaat gtcagcagtg
     3361 atttctatca aatagctgtc taattcgcct tgattccatt ctttaaagat atcagccatt
     3421 tcttctactg aaaggtttaa aatattcttc attaagtcat aagattcagc aatcagttgc
     3481 atatctccat actcaatacc gttgtgtacc attttcacat aatgtccggc tccatttggg
     3541 ccaatatatg tgacacatgg tgctccgtct tctgcttttg cagaaatttg ctctaagatt
     3601 ggtgcaacta gcgcataggc ttctttttgt ccaccaggca tgattgaagg tccttttaaa
     3661 gctccttctt caccaccgga aacgcctgta ccgataaagt tgatgccaga gttggctaac
     3721 tcttcattac gacgcatcgt atctttaaag aatgtatttc ctccgtcaat caaaatatcg
     3781 cctttatcaa ggtgaggaag taactcttgg attgttgcat ctgttgccgg tcctgctttg
     3841 accattagca agatccgacg tggtttctcg atagattcta caaattcttc gattgtatat
     3901 gttgctttga aatttttatc tgggtgttct tctactacag cggttgtttt agcccctgta
     3961 cgattgaata aagccactga atagccgcgg ctttctatgt tcaaggctag atttttaccc
     4021 ataacggcca taccgacaac gccgatttgt tgttttgaca tatgaactcc tcctacatac
     4081 gggatttctc cctaatctct tacattatag cttaacacgg ctacattgca aactaaagaa
     4141 caacatattt tttatttacc gactacaaaa aaataaacct caaggctcca tggttagcct
     4201 tgaggtttta cattttctaa ttaaaatgta ttaagcttct tttgacatta cgtccttacc
     4261 atcatagtaa ccacaatttg cacatacgtg atggcttttt ttcatttcac cacagtttgg
     4321 acattcgttt aaccctttga ttgtcaattt gtagtgtgta cgacgttttg cttttttagc
     4381 ttttgatgtt cttctagctg gtactgccat tttgctacac ctccttgtaa agtcttgtat
     4441 ctaaattaga cacaatatat tccaatctta cttgttatcg tcgtcttttg atttagaatc
     4501 aaaaagttca gatagttttg caagacgagg atcaatttta ttctcggctt cttccatttt
     4561 tctgcgctgg tattcttctt cggaaacaac ttcccaatca ttgccttttg gaagttctgt
     4621 tgctgcttcc tcttcttttg tcaatacccg cattggaatc gccaataaga tgttgtcttc
     4681 aatcgactct gctaaattca ataatggacc ttcaattagc aaaatctcat ctgcagacat
     4741 ttgttcatct ctactttgga attgttcttc tgtcatgaat acttcgtcta cagaaaaatc
     4801 catgggcagt tcaacaggtt ctagtgaacg tgttgaagga actgtcaaag tcgtctgcac
     4861 agtgtaatga acgatatagc ctttcttatc aactgagacc aaaccttcga cttccactgg
     4921 cgcaacatca agtattaaat tgtctcgctt catgagagcg gctttcaagt cgagcgtctc
     4981 atgaaaagtt aacggtgtct cctggtattt tctcaattcc aataaagacc atttcatttt
     5041 tcatcactcc taagcaacaa aagcaattat acagggcaaa aagcatgttg tcaatgaaat
     5101 tttcttgaca ccttgcagtt cataaaaaaa cagaaaaatc tttattagaa agcctctgtt
     5161 tgctatcatc ctcagaatca tttacaataa tcattattcc attaaaggag cggatgatct
     5221 tcatgaacac gaaagatatc gaatactatg tccaaatcgt caaacaaaaa aattttacga
     5281 aagtggctaa ttatttccgt gtgagccaac ctactattac atatgcatta caacgtcttg
     5341 aaaaagagtt tgacaccgta cttattacaa gagatcgatc gcatcacgaa ttgatcgtca
     5401 cgccttctgg aaagcagctg tttctccatg cacaaaccat tctccaagag atttctactg
     5461 caagagctga aatcactcag ttgcaaaaga aaaagcttcg ttttggcatg cctcctatta
     5521 tcggcaatga atactttcct aaactgtcaa gttactttat caagcatgaa ttgatgaatc
     5581 aaattgagat taccgatgga ggctctcggg acttatacgg gatgatccga caagggagga
     5641 tcgatttagc tgtattaggc tcaacacagc caatcgtgga tcaatattta gaaacagagt
     5701 tacttttgga caaacgtttc atgatcgtcg tgccacctac acatcctctt tccaagcagg
     5761 atactgtctc tttcaaagat atagcagatg aacagtttgt actattgaac gaacattatg
     5821 tccaccccgc agcattcact aagctttcta aacaaacaca tactcatcca aaagttgttt
     5881 atcaaagcaa tgacttgacg attttaaaga gtatggtccg agaaaatgtg ggtatcggtt
     5941 ttttggcaga gattgctgtc catcctgaag atcatttagt ttgcctccct ctttcagatg
     6001 aagttcagcc tcgcttcttg atttcattgg caaaacgaac acagcagatc gtcacgcctc
     6061 ttcaagaaca agtagtccaa gttatccatg aattcgttac agcacaaaaa aacagctctt
     6121 cgtaaaagaa gagctgtttt tattttttat taagctaaat tagatagctg ttgtttcgcc
     6181 acggtaaacg atgccgcgac gtgaatcaac ggtaatcaat tcaccttctg taattgttgt
     6241 tgttgcgcct ttagcaccaa cgattacagg aatatcttgt gcaattccta caactgcagc
     6301 gtgagaagtc aatccgcctt cttcaacgat aacagcagct gctttttcga ttgcaggcat
     6361 atattctttg tcagttgatg gtacaactag aaccattccg tcttttgctt tttttacagc
     6421 ttcttcagca gatgaagcaa taactgcatt tgcaacgaca gatttttgtc caacgccttg
     6481 agcttctaat aatttagaac caatcatttg aattttcatc acgttagttg ttccgcgttc
     6541 accaactggt acaccagcag tgatcaagat taaatcgcct tctgatgcga aacctaattc
     6601 aacagctttt ttagctgcta gttcaaacat ttcatcagtt gttgatggtt tttcagcaac
     6661 tgttgggtaa acgccccagt ttaacatcaa accacgtttt gtgcgttcgt caaaagtaac
     6721 agccaagata tctgcatctg gacgatattt tgaaatcatt tttgcagtgt atcctgattc
     6781 agttgcagca acgattgttt tcacgcctag attttttgca gcacgagcta cagataaacc
     6841 gattgtttca gttacatctg ttttatcgaa ttcgttcaat tggaatgtac ctaattctga
     6901 taatgcattt tcagcttctt ggtcgatacg tgccattgtt gaaacagctt caactgggta
     6961 gtccccgttt gcagattcac cagatagcat tgttgcatct gtgccatcaa atacagcgtt
     7021 agcaacgtca gaagcttccg cacgtgttgg acgtgggttt tcttgcattg attctaacat
     7081 ttgtgtagca gtgataactg ggataccagc agcattacat tttttgataa tgcgtttttg
     7141 aaccattggt actagttcag ctgggatttc aacacccata tctccgcggg caatcataac
     7201 accgtcagaa actttgatga tttcgtcaat gttgtcgata ccttcttgag attcgatttt
     7261 agggaagatt tgaacatgag tcatgtcttt ttcttctaag atttcgcgga tatctagaac
     7321 gtcttgtgct ttacgtacaa aacttgcagc gataaagtcg atatcgttgt ctaaaccaaa
     7381 acgaatatcg tcagcatctt ttggagtaat acctggcaaa ctaatagaaa cgcctggtgc
     7441 atttacccct ttacgtgatc ctagcatacc tgcattttta acttctacga ctaattcacg
     7501 gtttgcttcg tctttttcaa tgatttgcat atcgatcaaa ccatcatcaa ataaaatgtg
     7561 tccgccaacg tgaacatcgt caaataatcc tgggtaagta accgcgattt tttcttttga
     7621 acctacatgt tcaggatcca tagagatgcg tgtttgatct cctacttcaa atttgatgta
     7681 tcctgcacga ccaaaatcag cttctgttgt gccttgaaca gttgtacgga tttcagctcc
     7741 ttttgtatct aaaagaatac cgacatcttt tcctgtcttt ttaactgctt cacggaccat
     7801 agtcatacgt gataattgtt cttcatggtc accatgagaa aaattaaagc ggaagacatt
     7861 tgccccagat tcaatcaatt gagaaataat ttctactgag ttacttgctg gtcctaatgt
     7921 actaacgatt ttggtttttt tcatttaaaa aactctccca ttcaattttg tttatttaaa
     7981 gctgtacaca gcttaaatag cttggtttct ttcactctta aaatgaaagt gaattattta
     8041 gatcatatag tgataagtct ggtttatgtt tgttgttttc aagtgtatca atgatgtcag
     8101 acgcaacgat ttcgttgtca tggataccga cacataatcc accttttcct tcttgaagca
     8161 attcgacagc gtaagcaccg aatttacttg ccaacacgcg gtcgcgtgcg cttggtgctc
     8221 caccacggac aacgtgtccc aagatagata cacgtgtatg atagtcacca aattctgaaa
     8281 gtttctcagc gaattcattt ccgcccatta caccttcagc caagatgatc aagcaatgtt
     8341 tcttcccgcg atcgcggcct tcttggattt ttttagcaac tgaagccata tcaaaatcat
     8401 gttctgggat gatgatttca tccgctccgc cagccacacc tgaccataaa gcgatgtctc
     8461 ctgcattgcg tcccattact tcgataacga atgtacgaac atgagaagtc gctgtgtcac
     8521 gaatacggtc aattgattct agtactgtat tgattgctgt atcaaaaccg attgtgaaat
     8581 cagtcccagg aatatcatta tcgattgttc caggaatacc tactgctggg aaaccgcgtt
     8641 ttgtcaaagc cattgcgcca tgataagagc cgtctcctcc gataaccact agaccttcga
     8701 ttccgaattt tttcaattgt tcgattcctt ttaattgacc ttcttcagtt gcaaattctg
     8761 gataacgtgc agaatacaag aatgtaccac cacgttggat cttgtcacct acatctgcca
     8821 catcaagacg acgaatatct ccagcaacta aaccggcaaa accataattg atcccgtata
     8881 cttccagtcc ttcgtaaata cctttacgaa caacggcacg aacagctgca ttcattccag
     8941 gtgcgtctcc gccactggtc aaaattccga tgcgtttcat aataaacatt cacctcatta
     9001 aaatacttta tcctaagtat gacttaggtt tacaaccatt tttgcgttaa ccacgactaa
     9061 cattctaaca ttatttttct cacttgtctt gatttacgga taaaaaaatg aaaaaaaatg
     9121 aaagcaagta gtatttttta ccgtaagtta tttgaaaacc acattatctt cgccaagcag
     9181 ataaataagt tgccgcttta tactcttgct gtcatccacc caataagcat ctgacagaac
     9241 tacttttcgt tgcgtttttt cgtgatacat aactactggt atatatcctg ggtgtttctg
     9301 aaaaatcact tgtaattgtt tcattatctc gggatcttcc cgttcttctg taatacgcaa
     9361 atagcaagtt tgtgaaggat actgggcttc taactggtta ggatcagcaa agctttctcc
     9421 aattagttgc agttcctgat tatatttgct gatttcgctc cgcccttgaa caaagatcac
     9481 tcgatcaacg gcaaacaaat tacgatattt acgataaaga gttggaaaaa ctgtgacaga
     9541 aatttctcca ctactgtcgg ttccttcaac aaaggccatc agttctcctt tttttgtccg
     9601 aatttctcta acttccttga tcatcactaa taaattgact ggttgattag ggacgatatc
     9661 tgtaatcagc tgaacttgtt tagctaaacg aagacgatca aacccttctg caggatgacc
     9721 tgacaaataa atacctaagt actcctcttc cagcttcaag cgttcttcta gtgtatagtc
     9781 agcgacttct tcttctttta atgccatgat tcccagcaag tcaagacttc caccactgta
     9841 aacgatattt tggatttttc cttcgagatc aagcatcaat tgtttcctgt taggagataa
     9901 ttcatcaaaa actcctactg ctgtcagtga ttgcagtaac tctaatttca gccaacgctt
     9961 atcgatccgt atgaggaatt gatccactga ctggaacggg ccattttgtt ttctttcgtt
    10021 taatatatct tctacaaaat cacgccgaat accttttatc gctgtcagtc cgaatctgat
    10081 ttcttcctta ttgaccaact caaaactgga attactttga ttaatggacg gcgaggaaag
    10141 tttgagttta tttttacgtg cttctgcaat atattctttc aattttgatg tgttatgccg
    10201 aacagaattc atcaggctgg taaagaaagc acctggatga tgggctttta gatacgccat
    10261 ttggaatccc acgaatgaat aagcaaatgc atgtgaccga ttgaatccat agtttgcaaa
    10321 tttttcgata tattcatata cggtcgctgc tgttttttcc gaatatccct gttgtaatgc
    10381 tcctgataca aaatgcgact tttcttcatc taatacgtct tttttctttt tactcactgc
    10441 ccgtcgcaga atatctgact gccctaatgt aaatccagcc atagtcgagg cgatctgcat
    10501 gatttgttcc tgatagacga tcactccata tgtgtttttc aaaatcggga tcagtgaatc
    10561 atctgggtag cgaatggctt cttttccttt cttcctggca atgaaggtat ctatattttg
    10621 catagggcct ggtcgataca atgcattgac tgcggcaata tcttcgatgt tttctggatc
    10681 taatcttcgt aaaacgttcc ggataccagc agattcaaat tgaaatactc ctgaggtttc
    10741 gcctcgttga aacaagagca gcgtttttgc atcatttagc gggatctctt ttaatcggat
    10801 ctcataccct tctactcgct taacagcttt caaagtaaaa tcaataatcg ataagttgcg
    10861 tagtcctaaa aagtccattt tcaaaagacc gattgcttct acctcattca tcgtatactg
    10921 ggtcagaaaa gcttcatcag aaccgcgttg gagcggaact agttcaagta gattcttatc
    10981 gctgataact acacctgctg cgtgagtcga aacatgtctt ggcaatcctt ctaaaatcac
    11041 tgcagttttg aataaaagtt ggtttttttc gcttgcctga actaattcta ctaattgatg
    11101 cgaagtttta taagcttggg tcaatgtgat tttcaattga ttgggtattg cttttgacca
    11161 gcggtttgct tcactttgcg ataaaccaaa cactctagct gcatctctaa gaaccatttt
    11221 tgctgccatt gtgccaaatg ttgcgatttg tgccatatga tattgtccat acttttctcg
    11281 aacataattc aatacttctt cacgtcgatt gtcaggaata tccaaatcga tatctggcat
    11341 cgtaaagcgt tcggggttta aaaagcgttc gaacagcaaa tcatattcta atggatccac
    11401 atctgtgatt tctaacacat aggctactaa tgaacccgca gcagaaccac gacctgcacc
    11461 ggtaacgatt ttgtttctat gagcaaaagc cattacatcc catacgatca agaaataatc
    11521 atcgaatccc atttgatgga tgacagataa ctcgtagtcc aaacgttctt ggtattcctt
    11581 tgtatagtca gtgattcggc gttccacttg cgaatagcat aactctctta aatattctcc
    11641 agcttgtttt ccattaggaa ctggataatg cggaagtaat ttctgatgga actggaattc
    11701 aaatgaaatc gattcagcta aacgctgggc gcctattaca gcttgggaaa gacccgcttt
    11761 ttcgaatcgt ttgatcattt cttgttctga aagaagatag acatttgctg ttagtggtaa
    11821 agatgcagat aaattttcta aaggtatttg gcttccctct ttgatatgtt tcatcacttc
    11881 aagtgcaaaa agttcttctt gttgtaaact agcgatggga tgtaatgcac aaagcgaata
    11941 attttctttt atcacatact gataccattc aggcaattga tcaggcgtgt tttctgtcaa
    12001 gccgatataa aaatttctag taaaaacatt ttcaagtaaa aactgccaat gaccgaaaaa
    12061 gttatcctct ttattgaaag cctcagaaac agaatgattt gccggaacaa caaacgctag
    12121 atggtcggtt tctgtcattt ctgcaagttc caatttttct tggatcattt ttcggctgga
    12181 aagtttcatc agctgctgat aacctatttc atcttttgcg tacgcaagca agccataatt
    12241 tttttcttca taagtataac taagatgtaa tccaatgatt ggctgtactc cttcttctac
    12301 acaaatacga tagaattcag gtgcaccgta taagacatct ttatccgtta tggctaatga
    12361 agtgtagcct tgtttttttg cttcttgtac atattctcga atacgtatcg tactttgtaa
    12421 aagagaataa gaagtaattg tataaagttg tgggaaaaac atcttgcatt ccgccttttt
    12481 tttcgttaaa attcactagc gttaaaaggt ttgatccttt tactttcatt aagatagcag
    12541 gcgctgaagt tgattattcc acttcatttg cttgcttgta atctgtagga atgagttctt
    12601 tcgcaagaac tcaaatttta ttgagatttg gtctcgtttc tttacattca aagaaaatct
    12661 ggtaaaatta agatagaaaa gaggtgttct ttatgcgata tatcgttaca ttattctggg
    12721 ctgtcgttct aggtcaagta gtcggttata ttggtgctgc attaacaagc ggaacatatg
    12781 attttacttt gacaacaatc atttcattca ttgcgggtgt cattatcctt ttgattggtg
    12841 cagttgcgcc aagaaaagaa acctctgccc attcgtaaaa aaacagccaa gagcttgaga
    12901 tttctcaagc tcttttttat tttctactcc tcaccaccat tataatctgc tgcgaagaac
    12961 tttacgaaag ttgaataaag aaagaagaat atttgacaga acaaacaaac ttgtcacaat
    13021 aaaaacagca ctgtaattat aagcatgcgc gactgtcgat cctagcatgg gacctaaaac
    13081 ttgtccaaag ttgctaaaca tctgattgta gctatagatt cggctgactc cttcttgagg
    13141 cgtaatctta ctgatcagtg tattgacaga aggcatcaaa gcgccagttg aaaagcctaa
    13201 aataaagcga agtataccta attggagcgg tgtagtgacg aatgccatag gtaaataaca
    13261 gcaaaatgat aaaatgagtc ctccaattag aactttttga ttcccaatct tatcccctag
    13321 ttttcctagt ttcggtgaag aaaagacagc ggaaacacca gctatcgaaa caatcaagcc
    13381 gctgacaaaa aggatatttt ctgtacttcc gctcaattca cgaatatata aggtcaaaat
    13441 agggctgatc gtagtgattc ctatctgtaa aatcaaggtc gtaatgaaaa gaccgatcaa
    13501 aatagagaga tgatccattt ttgaaaaaat ttctttggtg cttatcagat cttttttctc
    13561 gattggatgg aaatcttctt tgactaagaa aatggtcaat accgttgtga tcatcaacaa
    13621 agcccctgtg atcaagaata cattttccat accaaaccac tgagccagtg caccgcccat
    13681 tgaaggtccg atcagatttc cagcaatcgc tccggtagac aatgttccta acgcccatcc
    13741 gctcttttct ctaggtgcct gtgaagcaat catcgctgtg gcatttggaa tatatccaga
    13801 caaaatccca ttccaaaacc gcatgatcaa aagccaatac gcgtttggta caaaagccaa
    13861 agatcccatt gttaatgtca tccctgctgc tgcacggatc atcatgagtt ttcggccttt
    13921 tcgatcagct agatttcccc agattggtgc aaccacggca gatgcaaaag cagtcacaga
    13981 gattgctagt cccgaaaaca attcgatttg atcttttggt gttcctagtt gctcaacata
    14041 aactggtatg aatggcataa ctaaactaat acttgcacca gtgaaaaaac agccaatcca
    14101 cgcaatcact aaattttttc tccaattaat ctccatctat catccctctt tcgtctcttt
    14161 tatcatacct attttcgaat aaacaagcaa cgatctcgcc agtaaaaaga gaaaaatccc
    14221 gcaaaggtca ttttatatgt aacgttcttt ttatgtaaaa caaaaagaga tcagaacaaa
    14281 tactgttcca atcccttctc tttagttaaa ttgttgctag aatgatgaag ttgataatga
    14341 ataaaatatc aacgatccac aaagcaacag aaacttgatt gtattttcct ttgattactt
    14401 tcacaattgt atagaaaata aatcctgctg caatcccata ggaaatgcta tagcacaatc
    14461 ccataaagat cgatgcaaag aaggccggta aagcttcttc catatctagc catttgatgt
    14521 ctgcaaaaga agccatcatc attactccaa ctaaaatcaa tgctggcgca gttgcttgat
    14581 ttggtacaat ggcgatcagt ggagagaata aactacttaa tgcgaatagc actgccacta
    14641 ctactgaagt caaaccagta cgtccaccgg ccccgatccc agctgcagat tcgacataag
    14701 ttgttgtatt cgatgtacca aagatagccc ccacagaagt agcgatcgca tcggcaaata
    14761 gtgctttatc catttttgtt ttgaacccac gaccatcttc caacgcgatc tcgtcttctt
    14821 ttgagaaaat acctgtgcgg cgtcctgtac cgataaatgt tccgatcgta tcgaaagtat
    14881 cagataaact gaatgcgatg atcgtcatca atacttgagg aatcttggaa gaatcactga
    14941 ataaagattg catgccttca gaaccaaatg ctgcaccgaa agttgttcct aattctttaa
    15001 atgaattacc taacgaattc gtttgccaat caatggaact taaatcaaca acacccataa
    15061 aaataccaag gactgtggtc gctacgatac cgataagaat agccccacgg acatttttta
    15121 caactaaaat ggctgtcagt accaaaccaa tcacagccaa taagatagga gcgttgtcaa
    15181 agttggctaa cgcagggacg attccaccat ttatcgtaac atttgtcgct tttccgcctt
    15241 caacgacaga gctcgaaata gctgactggt ctgctgaaaa agataagaat cctgcatttt
    15301 tgattccgac atacgcaacg aaaataccga tcccgccacc aatcgcatgt tgaagacttt
    15361 cgggaatcgc ccgaataatc atttttcgaa tatttgtgac agtaataaaa atattgatta
    15421 atccgcaaat aaacaccatt gccaaggctt gttgccaaga ataaccaagc ccaaaaacga
    15481 ccgtaaaagt gaaaaatgcg ttcaatccca taccaggtgc ctgagcatat ggtacatttg
    15541 cgaacaatcc cattaccagc gtaccaataa tcgatgcaat gattgtcgct agaaatactg
    15601 cttggaaggg catacctgat gctgacaaaa tcgtcggatt tacgaataag atataactca
    15661 tggcaaagaa tgtcgttact cccgccatta tttcagttga tacatttgta ttgtttttct
    15721 ttaaaccgaa aaacttttcc ataaaaagtt ccactccctg aatttttttc tgatggaaaa
    15781 ctataataat aaaaaataag taaaataggg caaatccatc gaacaatttc aaattatata
    15841 gacgtttcat tcgcttttca acgttttttt aacaaaaaaa gcgaaaataa tacttaacgt
    15901 tcgtgtttag cttatgttta gctatttata tagccataac tgtagttttt gttaggaata
    15961 gctgattgct cttgttaatt acctaaaata tgctagacta aaacaaatgc tagttgagga
    16021 aggaaggacc cttattgaat aaaaaattaa ttgccatcga tctggacgga actaccctca
    16081 atcaagattc gatgattaca tctaaaacaa aagagactct aaaaaaagca atcaatgccg
    16141 gtcatcatgt cagtattgct acaggacgcc cattccgaat gagccatcag ttttaccaac
    16201 aattagaatt aacaacaccg atggttaatt ttaacggcgc attagtacac atccctaatc
    16261 agcactggga tggtgaaaga gaaacgttga tcaatcgtga aatcgctttt gaaatcttat
    16321 cgcaaaaaaa gcagctaaat ttagatttta tcgctgctga aaatcgtgat acttttttta
    16381 ttgacagttt tgatttcttt gatgaaaaaa tttttgcttc ttctcgtcca ggagagaaga
    16441 atttgctttc tcctaaaaat ttgacgacaa atcctacatc tttgcttgtc cgaacagaca
    16501 aacgttttgc aggagctgta tctgctgaat taacacgtca atttggcagt tatgtcgatg
    16561 ttcggacatg gggtggacct actgccatct tagagattgt ttcaaaagga atccaaaaag
    16621 caaaaggcgt gcaagagatt gctaactacc tgtctatcga tcaaaaagac gtcattgcct
    16681 ttggtgacga gcataatgat ttggaattgt tggattatgc aggttgggga gtagctatgg
    16741 ctaacggtac tgaccaactc aaaggcattg caaatgatgt cactcctttg agcaatcagg
    16801 aagacggttt agctgtttat ctggaaaaac tacttaaact ataatagaga attagattcc
    16861 acaaaaaaga ggttgtgaca atttt
//
LOCUS       DAZKCY010000054        16483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000054 DAZKCY010000000
VERSION     DAZKCY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.054"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..1151
                     /locus_tag="ACG8XC_001613"
     CDS             12..1151
                     /locus_tag="ACG8XC_001613"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFP9191309.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1144..1944
                     /locus_tag="ACG8XC_001614"
     CDS             1144..1944
                     /locus_tag="ACG8XC_001614"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9191310.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1944..2771
                     /locus_tag="ACG8XC_001615"
     CDS             1944..2771
                     /locus_tag="ACG8XC_001615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9191311.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNLAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2776..3483
                     /locus_tag="ACG8XC_001616"
     CDS             2776..3483
                     /locus_tag="ACG8XC_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9191312.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            3583..4449
                     /gene="rfbA"
                     /locus_tag="ACG8XC_001617"
     CDS             3583..4449
                     /gene="rfbA"
                     /locus_tag="ACG8XC_001617"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP9191313.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4463..5035
                     /gene="rfbC"
                     /locus_tag="ACG8XC_001618"
     CDS             4463..5035
                     /gene="rfbC"
                     /locus_tag="ACG8XC_001618"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFP9191314.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5057..6085
                     /gene="rfbB"
                     /locus_tag="ACG8XC_001619"
     CDS             5057..6085
                     /gene="rfbB"
                     /locus_tag="ACG8XC_001619"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP9191315.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6183..7034
                     /gene="rfbD"
                     /locus_tag="ACG8XC_001620"
     CDS             6183..7034
                     /gene="rfbD"
                     /locus_tag="ACG8XC_001620"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFP9191316.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7069..9102
                     /locus_tag="ACG8XC_001621"
     CDS             7069..9102
                     /locus_tag="ACG8XC_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191317.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9146..10426
                     /locus_tag="ACG8XC_001622"
     CDS             9146..10426
                     /locus_tag="ACG8XC_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFP9191318.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10636..11442
                     /locus_tag="ACG8XC_001623"
     CDS             10636..11442
                     /locus_tag="ACG8XC_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9191319.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11454..12674
                     /locus_tag="ACG8XC_001624"
     CDS             11454..12674
                     /locus_tag="ACG8XC_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9191320.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12664..14250
                     /locus_tag="ACG8XC_001625"
     CDS             12664..14250
                     /locus_tag="ACG8XC_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9191321.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14289..16427
                     /locus_tag="ACG8XC_001626"
     CDS             14289..16427
                     /locus_tag="ACG8XC_001626"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9191322.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         5222 a         2727 c         3507 g         5027 t
ORIGIN      
        1 taggtgccca tatgcttgtt tcttttagta tggttataag attttttctg acaattttat
       61 tatcactgat attaacacct gtattcaaaa tcatttcagt acaaacaggt atggtcgaca
      121 aaccaaatga aagaagaata aataaagttc caatgccttc agcgggcgga ctgcctatct
      181 ttatttcatt tgttatatcg actttattct tattccgtaa aattatccct caatcgtata
      241 ttgtaccgat cttgattgct tctttagtaa tcatagtcac aggagtgctc gatgataaat
      301 atgagctgac acctaaacaa aaaagtatag ggatcttgat tgcttctctt attatttatt
      361 ttgtagcaga tatacgtatt gattccttca cgcttccttt tattggatat atccaactag
      421 gctggcttag ttttcccgta acgatttttt ggatcttcgg tatcacgaat gcggtcaatc
      481 tgattgacgg tttggatggg ctggcggcag ggatttcatt gatcggttta attacgatag
      541 gaatcattgg atattttttc ctccatgctt ctacagtgta tattccagtt gttattttct
      601 gtcttgtagc aagtattatc ggtttttttc cttacaattt ttatccggca aagatttatt
      661 taggcgatac aggagctcta ttcctagggt ttatgatggc cgtcctttca ctacaagggc
      721 tgaagaacgt gacttttgtt tcatcgattt ctttgctgat cgtcatggga gtgccggtaa
      781 cagacacgtt ttttgctatc attaggagaa aagcaaaccg tgtttctttt tctacagcag
      841 ataaaaaaca tcttcatcac cgactaatca gtttaggctt tactcataaa ggtgctgtcc
      901 tgacgattta tgcgatggct ttgatgtttt ctttcacagc tatggtcatg aattatactg
      961 gaagaatcgg aacgatcata ttgattattg ccatgttgtt tgctgcaatt ttattatttg
     1021 aattgattgg tctgatcaat gagaaacatc agtttatttt aaaaacattg cgatttttag
     1081 ggaataaaga gtaccgtacg caagtaaaac agaaatattt gaagaaatgg aaccgtcata
     1141 tagatgaata acaaattaaa ccagccgatc cttattagta ttgtgacggc aaacagtgat
     1201 aaaatttttc ggacactaga taattttatt tcaactgtaa aagatgaaac agcagtaaaa
     1261 atccgaattt ttgacaacaa ttcacttccc gagtacaaaa aaagactaac agagtatagg
     1321 aaatatccct acatcgatat ctattttcat gatgaaaatt ccggatttgg ttatggacat
     1381 aatcacaatc tgatagctag cgattctcgc tatggcatta tttgtaaccc agatatttta
     1441 gtggatgaag agacagttac agcattggtc ttgttattga aagaacatcc ggaatgtgcg
     1501 atgttagcac ctaagattct taatgaagat ggaacaactc agcacctcat ccgtgagcag
     1561 ctggctgttt ttgattacgt tttgcgcttt gtgcctttca aatgggtcaa aaaaaagttt
     1621 gacaaacgac tggctgtgtt tgaatgccga ggattgccta ctgatgctaa tagttatgta
     1681 cggatgatct caggtagttt tatggtagct gatattgata aatttaaaga gataaagggg
     1741 tttgatgagc gtttcttcat gtactttgaa gacaacgact tgtgtttgag ttttgaaaaa
     1801 gcaggttaca agttattgta cacacccttt tatgcagtta tccattttta cgggaagggt
     1861 gcgcatcgaa atgcaaaact gtttcgcgtg tttttgactt ctatgatgaa atttttcaat
     1921 aagtgggggt ggcgtttttt ctgatgaaac cagaaatatt agtagtcgtt gtgctttatc
     1981 agcaactatt cagtcaatct ccagcttatg gaccattaag caaagcactt agcgaaaagc
     2041 aaattcagct tattatttac gataatagtc ctcaaaggca gcaaaatgag ctgtttgaaa
     2101 aagaaaatat cgtttatcat catgatccgc aaaatcctgg tttagcggtg gcttataatt
     2161 ttgcgttgtc tcaagcagat gaaggaacac gttgtctcgt cactctggat caggatacga
     2221 ggctagtcga cgattatttt gaactattgc gtaaagtaac ctttacagat gaatgtgttg
     2281 ctgcagttcc gatgattttt tctgggagcc gacaagtttc tcccctatat tcagatcgtt
     2341 atatcaatcg tacagctcaa gcagtagaag ttgaaggccc aacagctgaa cgaatcatgg
     2401 caatcaattc aggtgttgcc tggtctgtca aatttctaaa aaagattggc gggtttaatc
     2461 ttgctttttc tttagatttt ttggatcatt ggctgttttg gaaagtggat cagttggaaa
     2521 aaactgtgga gatccttcct gtacgaatgg aacatgatct ttcggtttta gactatgaaa
     2581 aagtttcaag taatcgatac cgttctattt tacaagcaga aaatcgattt tatcatgaat
     2641 atgatcaaag gcatttatcc agtcaccgac gacaattatt gttaagaaca gctaaacagt
     2701 tcttgaccgt caaaaaccgg catatctggc ggatgacttt gcgctcatat gttaagaatt
     2761 ggaaggtgtg aattcgtgcg ttcggtttgt atagctacct ttaatggagg cagatattta
     2821 agagagcagt tagatagtat tttatctcag ctttcagcag aagatgaagt agtcatttct
     2881 gatgatgggt cagtagatga tacatgggat atattaactg aatacgcaga aaaagataag
     2941 cgaatccagc ttttttcggg gcctcgtcaa ggattgatcg ctaattttgg ttatgcgatc
     3001 cagcagacga aaggagatct aatctttttg gcagaccaag atgatgtttg gttggagaca
     3061 aaagtgaaaa agatcactga ctattttgag cagaatccgg atcagctagt agtagtttct
     3121 gatttaatca ttgtggacag cgagctgcaa gagatccatg attcgtattt ttcgtttcga
     3181 aattccaaag aaggatggct taataatgtg ataagaagtc actatattgg tgctggtatg
     3241 gctttccggt cttctttaaa acccgatatt ttgccgatcc cgtcagaagt gccgatgcat
     3301 gatatgtgga ttggtttgct gggaggaaag aagactgggt tcctaagaga accgttaact
     3361 ctgtaccgaa gacatgattt aaacgcgagt gagattgaaa cacattctaa attacggcaa
     3421 aaaatttctt ggcgtgttca tctgattacg gcgttgatca aacgaaaatg gtttaaacgt
     3481 tgataaaaga tgtttaaatc cttaatattt ccatgacaaa tataacaatt gatgagataa
     3541 taacaaagtg aaatttgaaa tcaaaaagga gagcagatat ccatgaaggg aatcattctt
     3601 gctgggggca gcggaacacg tctgtaccct ttaacaaagg caacatcgaa acaattgatg
     3661 ccgatttatg acaaaccaat gatttattat ccaatgtcta ctctgatgtt ggcgggaatc
     3721 aaagaaattc tgattatttc aacgccacaa gatacaccac gtttcaagga attatttgga
     3781 aacggaaatg atttaggact gcagatcgaa tatgcagtac aagaaagtcc agatggattg
     3841 gcacaagcgt ttatcattgg agaagaattt attggagatg atagtgtctg cttggtatta
     3901 ggggacaata tttactatgg tggcggtctg tctaaaatgt tgcaacgagc agcttcaaaa
     3961 gatacaggcg cgacagtatt tggctatcat gtcaatgatc cagaacgctt tggtgtagta
     4021 gaatttgatg agaatatgca agctttgtcg attgaagaaa agcctgctca gccaaaatca
     4081 aattacgctg taactggcct ttatttttat gacaatgagg taatttctat cgctaaaggt
     4141 atcaaacctt cagaacgtgg agaactagag attacggatg tcaataaagc atatttggat
     4201 aaaggaaaac tatccgtaga acttatggga cgcggatttg cttggcttga tacaggaaca
     4261 catgaatcat tgcttgaagc atctaccttt atcgaaacaa ttgaaaaacg tcagaactta
     4321 aaagtggctt gtttggaaga aattgcttac cgtatgggct acatcgataa ggaacaattg
     4381 ctggcccttg cacagccatt gaaaaaaaat caatacggtc aatatttatt gaatttggcc
     4441 gctgaataag gagtccttac agatgaaagt aacagataca aaattaacag atgtcaaaat
     4501 cgtagaaatg gacgtctttg gcgatcatcg cggtttcttc acagaaagtt attcaaaagc
     4561 aaaatttgca gaacatggat tgaaatatga ctttgttcaa gacaaccatt cactatcagc
     4621 tgaagctggt gtccttcggg ggcttcattt ccaaaaagga gaagctgcac agacaaaatt
     4681 gattcgggta gtcactggag cagttttaga tgtagtagtg gatatccgca aaggtagccc
     4741 gacttatggt caatgggacg gatttatctt atctgaacat aatcatcgac agttgcttgt
     4801 acctaaagga tatgcccatg gattcgttac tttgacacca aatgtgaact tcctttataa
     4861 gtgtgataat tactataatg cagaagcaga tgggggcatc gcatttgatg atccagattt
     4921 agcgattgat tggccaattg atccatcaag agcgattatg tcggaaaaag atcaccgtca
     4981 tccgacatta aaagaatttg aagcagaaaa tccatttgtt tacggagaaa tctaagaatt
     5041 aaggagaaat tttcttatga aaaacatcat tgttaccgga ggtgccggat tcatcggctc
     5101 taatttcgtt cattacgtgg taaacaatca tccagaagtg catgtcactg ttttggataa
     5161 actgacttat gcaggaaaca aagagaactt ggccggactt cctagcgatc gcgtagaatt
     5221 ggtcgtagga gatgttgcag atgcagaatt agtggaccgt ctagtaaaag aagcggacgc
     5281 agttgttcat tacgcggcag aatcccataa cgataattca ttaaaagatc ctttcccatt
     5341 tgtacaaacg aatatcattg gtacgtatac acttttagaa gcttgccgca agtatgatgt
     5401 acgctatcat cacgtatcta ctgacgaggt ttacggcgat cttccattac gtgaagacct
     5461 gccaggacat ggggaaggcg aaggagaaaa attcactgcg gaaacaccat ataatccttc
     5521 aagtccatat tcttctacaa aagctggttc agatctgtta gtaaaagcat gggtgcgttc
     5581 ttttggctta cgtgcaacca tttcaaactg ttcaaacaac tatggtcctt atcagcatat
     5641 tgaaaaattt attcctcgcc aagtaacgag tatcttaagc ggtattcgtc caaaattata
     5701 tggcgaagga aagaatgttc gtgactggat ccatacgaat gatcattctt cagcagtgtg
     5761 gctgattttg acgaaaggca gaattggcga aacctatctg atcggtgcag acggtgaaga
     5821 agataataag accgtgatgg aattgatctt agaaatgatg ggccaaccaa aagacgcata
     5881 cgaccatgta aacgaccgcg cagggcacga tttgcgttat gcaatcgatt cgacaaaatt
     5941 gcgagaagag ctaggttggc agcctgagtt tacgaacttc cgtgatgggt tggctgacac
     6001 gatcaaatgg tacgaagaac atgaagactg gtggaaaaaa gaaaaagagg ccgttgaagc
     6061 agcatatgcc aaaaatggac aataatacag aggaaatttc actgtattaa tggaaaaaca
     6121 catgtgaggc aggcgctcgc ctgtctcttt tttaaaataa aaatcaagga gcggataacc
     6181 atatgttttt attaactgga ggaaatggac aattaggtac agaattgcgc catttattag
     6241 atgaaaaaaa tctgccatat gtttcaacag atgcaaaaga cttggatatc acagatgctg
     6301 agaaaacaat ggcttatatt acagaattaa aacccgatgt tattttccat tgcgcagctt
     6361 acacagcagt agataaagca gaagaagaag caaaagaatt agatgagaaa atcaatgtcg
     6421 atggcacaag aaatgtagca cttgcagcaa aagctgtgaa tgcacaattc gtctatatca
     6481 gtacagacta tgttttcgat ggaaaaaaca aaaatgtaga atacgaagta gacgaccaaa
     6541 cgaatccact aaatgaatat ggtcgtacga aattattagg cgaaaaagca gtagaagaga
     6601 ttttagaaga ctattatatc atacgaactt cttgggtttt cgggatctat ggacacaatt
     6661 tcgtcttcac gatgcaacgc ctagcggaaa cccgcgacaa actgactgta gtaaatgacc
     6721 agtttgggcg accaacttgg acacgtactt tggcagaatt tatggtatat gtcatcgaag
     6781 aaaaagcacc atttggtatt tatcatttgt cgaacgaaaa tagctgttct tggtatgagt
     6841 tcgcaaaaga aatattgaaa gatacagatg tagaagttgc acctgttacg tcagaagaat
     6901 ttccgcaaaa agcaacacgt ccgcagtatt cagtcatgag cctgaaaaag acagaagcgt
     6961 taggttttgt cataccaact tggcaagaag cacttgcgca aatgctggaa gctgccaaaa
     7021 aaatgaataa gtaatttcct ttttatcaaa tagaatttgg agcatgatat ggaaaaacaa
     7081 acaagaagtg cacgacgaaa agaaaagcca aaagcgaatc aggcagattc gaatttggtg
     7141 cttgacctgt ttctaaaact ccgctatctt attggcgttt tagtcatcgt ggtcatcgtt
     7201 acttttaatt taaacggaag ttcacttggt gtttgggata aatatgtgtc gcaaagagac
     7261 gatgggaaaa aaactgatgt gatttttgga cagaatcggg aagtgcggtc agacgaatgg
     7321 ctggtgcaga caccttttta cctctcacaa gctgaagagg acttttcgtt ggtgaacgag
     7381 gattattcat taaacggaca aaacatgatc attgcgtata attctcctgt aaaggatatt
     7441 acggttatcg gaaaaccatt caactgggga ttctttttcc tcggaagaga tcgaggactt
     7501 tccttttatt gggcgatgaa attagtcgtc atggtcttgc ttggattcga agtactgatg
     7561 atattaacga agaggaaaaa agctctatcg ctaatcggtg catttgcgct gacattttct
     7621 ccagcagtcc agtggtggtt catgcaacat gtgggcgatt tgattttctt tactttggga
     7681 ctgatgattg ctttttatca ttatttctac aatcatgata aaaaatggct acgaacgttg
     7741 atgatgcttt tggtggttat tttcggtatt gggtttattt tagtgattta cccagcccat
     7801 caagtgatgc tggcgtactt gctgattttt tattttgttg gcctgcttat ttactatagc
     7861 aagaaagttt catgggatct gttggatgta gtgttgattg gaggggcagt tttatttatt
     7921 ggcggagtca tggttcattt ctggctaacg tcaaaagacg cattgttagc ttctttgaat
     7981 accttatatc caggaaaacg ggtaagtact ggaggaaagt ggacgattgg cgaatttttt
     8041 tctttcctta caaattggaa aattcctttt gaagatatca actattcgaa taactctgaa
     8101 gtggccctgt tctatcactt ttttccaact gtattcctag caagtccatt tgtgttattt
     8161 ggcaaaaaag atagcgaaca aaagttactg ggacgtattt tgatgatttt ttgtctgttt
     8221 tctatctttt ggattaccgt tgggttgcca aaatggctag cagaaatcac tctgctgtct
     8281 tatgttcctc cattaagagc catccttact tttagttttg cggcttgttt attatcgatc
     8341 tggtttatcg cttatatttg gagagaagat gttattccta tttggtataa attcattttg
     8401 ttggcagtaa atgcatggtc ttatttctat gcgattacgc attcgaaaat ggataattat
     8461 ttttcggatt ttgaaaccat cgcagttgct gtgttgggaa cagtgatttt agcttttgca
     8521 ttatttggct ggagacgctt gttttacctc gcttttgtcg ccttagttat cgtatctggc
     8581 tttttcgtca atccagtcgt agaaggtaca ggagcgattt ttgaaaaaac actggcaaaa
     8641 gaaatccaaa aaatcgatca acaagatcct ggtaaagttt ggttgtctga agatgaatta
     8701 tataacttca ccccaacatt gggagtgaaa tccttcaata cagtacgctt ttatccagat
     8761 atagctgcat ggaagaaaat cgatccgaaa gaagaatacg agaagtatta taatcggtat
     8821 gctcatacaa gagcttttat cgctaaggaa gatacaaagt tcattttaga tcgtccagat
     8881 atgttttcgg tcacgttgaa ttttgaagac gcagaaaaat tagggatcga ctatgtgatc
     8941 agtaaacgac cattaactga ttacaaccaa ttatatgaag cgcagttcac gcagctatat
     9001 ggaccggata aagacggtta tatgattttt aaagtagaat atccggatta tccagatgta
     9061 ccacaaacac aaactcagga aaatcagcta gtcgtacagt aataggctag ctgagaataa
     9121 tcctgaaata ttagtagaaa ggagagtgaa gataattcaa gcaatcgtac aaaaatttgt
     9181 caacaaaatg agtgtcggtt tcttgctttt gttgctgacg ctagtctttt ttgtttggac
     9241 agcaggggta ggttttccag caactttgtt tatatataac aatagttcat tacttatcgc
     9301 ttttagtgtg gctattttgc tcattttaaa tgtttataaa gcaaaaggcg tcgattttct
     9361 ttatctaggt gttgcgcttg cccttttcat tttcttttct tactttcggg aattaagaaa
     9421 tgagtcttta tttggagact tactgtttcc tttgattatt ttattttgtt tagtaattaa
     9481 atggatcgag tggacaaaat gggatcgcgg tatttatctg attgtcttta cggtcttcct
     9541 cggaatcacg ttatatcgtg tatttacaga gatccaagta ccagaaggac aaagcatctg
     9601 ggctccaggc aattcgcttt ctgatatttg gatcaatacg aatacaatcg gaagttcttt
     9661 gatgactctt gctttattga tcagtggatt tgccagttct tttgaacgat ggtatgttcg
     9721 aatcatcggt gggatagcag tcgttgcagc ctttttaggt atttgggtct gccaatctag
     9781 aggtgcatta gtcgcgttga ttgtttttgc gatattggat cttttaccta aatcgttgat
     9841 gcgtgtcatt cgtgcgccat ttattgccta cacggttacg attcttttag ctttgccgat
     9901 ttcttatctg gcggctgtct ctgaaaaagt caatttgttt accggtcgag aagatatttg
     9961 gcataaattc tatcagacgc taggagaaaa atctgaacaa gttttactag gaatgaaaac
    10021 atttatcttc cagcgaggaa accaattttt aggaaaccac aatagttata attctatttt
    10081 gaatatctat ggattgatag gatttgggat tgctgctttg ctgcttatcc tttttattgg
    10141 tcgcttaacg ttgaaagcag atttatccaa cgggcagatg acttttatct gggccttttt
    10201 tgcggtgatg atgcagtcat ttatggagga tacattgact tcataccgat gggttccgat
    10261 tatttatatc ctgttggcta tggccgttca tcgatatgat gaaccagaag ttacggagga
    10321 attctatgtt atggaagagg catatgacga tatgacttat gatgatacat cttatgagga
    10381 acaaccagat agcgctcatt cttccaggat ccaacgctat cattgatagg cacaaaatac
    10441 gagagaggct gtgacaaccg tcgcagcctt ttttggttta ttgcaattgt ctagcatctt
    10501 ctgtaaaata gaaacgttag agaaaatctt gtatcataga aaattaagaa agaaataaga
    10561 acgcagctcg gtaccaaaaa tatggtaaaa tggcacttga ctgtaattca ctgaaaatcg
    10621 cttggaggaa cacaaatgtt taaagatttg tatttattta tgaaggacat ttatcagaac
    10681 agaaaattac tgctacagtt ttcgatcaat gacttcaaat caagatatgc aggatcgttt
    10741 ttaggaatct tatgggcgtt tatcaatccg ctatttactg tattgattta ttggctggta
    10801 tttggtgttg gactgaaagc acaaatgatc gatgcaaaat acccatttat cgtatattta
    10861 gttaccggga tgattccctg gttcttcttc tcagatgcat ttgcatcaac tacggttgtt
    10921 tttcgggaat atacttattt agtaaagaaa gtcgtattca atattcgtat cttgcccacg
    10981 acgaaaatcc tatccaatct gtacacccat ttattcttca ttctgattgg catagctgtc
    11041 gcgcttataa atggtatata tccttcagca atgtctttac agctgattta ttatttattt
    11101 tgcatgtgtg ctttcttgac aggattgact tggctgacgg catcgatcca accattttta
    11161 cctgatatca tgcaattaat tacgatcgtg ctgcagttga tcatgtggac attaccgata
    11221 ttatggagcc ctaatcagtt tgattcaggt cttgtaagca tattgaaatt aaatccgctg
    11281 tactatgttg ttcaaggtta tcgtgagtca tttttaagtg aagcttggtt ctgggagcat
    11341 ggaacatata ctttgtattt ctgggttttc acgttagtaa tgttcgtaat cggctctgca
    11401 gtgttccgta gattgaaacc acatttttca gatgtgctgt aaggagaata gctatgacag
    11461 aatatgcaat tagactaaaa gatatcacaa aatcttataa tatgtacgca aaaccttcgg
    11521 atcgatttcg tgaagccctc aatcctttta agaaatccta tcatgatgta ttttatgcgt
    11581 tgaaaaacgt gaatgtagaa ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg
    11641 gatcgggaaa atctacaatg ttgaaaatca ttacgggagt actgacacca acatccggca
    11701 cgatggaaat cgaaggaaaa atctcagctt tgctagaact aggatcagga ttcaatcctg
    11761 agtattctgg ctatgaaaat atctatttaa atggaatggt tctaggattt tcaagagaag
    11821 aagtcgatga aatggtcgat gatatcattg aatttgctga catcggcgac catatttatc
    11881 aacctgtaaa aacctattca agtgggatgt tcgttcgtct agcttttgct gtagcgatca
    11941 atgtgaatcc tgatattttg attgtggatg aagcattggc agtaggagat cttgaatttc
    12001 aactaaaatg tatggaaaaa tttacagaaa tcaagaactc gggaaagacg attttattcg
    12061 tttctcatga tattagttcg attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg
    12121 gaaaagttgt cgaatatggt gatacgatgg aagtaacgac gaattacgaa aactttttga
    12181 agaaaaaatc agttaaaaca gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc
    12241 cagatatcgt agacgtgacg aaagccgaat tgttggataa agataaaaat ccagtggata
    12301 tgctggaaca aggagaaaaa ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa
    12361 tcaaaaatcc agttttagga attgctctgc gtacagtcga caatttttat gtgtgcggac
    12421 tcaatactct actagatggt atcaagatcc cttggaaaaa aggtgagaat atcttttatc
    12481 tggagtatga aaagttaggt ttgttagctg gtgaatatta ttttgacgtt gcggtctttg
    12541 aagaaaatgc aacggttcct ttggtataca agacaaaata catgaacttg tttgtcagcg
    12601 gttcttatat cggagaaggc atcgtggtct tagaccataa atgggaagaa gggactcata
    12661 gcaatgaaat atgattttga aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc
    12721 gcacagatca aaccaggaag taaagtttta gagtttggtc ctggaaacgg ccgattaacg
    12781 aaacatttga tcggagcaaa acaatgtgaa gtgagcattg tcgagctaga taaggaattg
    12841 ttcgattttg tcagcgagtt tgcccaagat ggattttatg gagatatcga aagttttgaa
    12901 tgggcaaact attatgctgg acaaacattt gattacgttt tgtttgccga tgttttagaa
    12961 catttggtag atcctggaaa aacgttgaag aaagttagag aatttttgaa tgaagaagga
    13021 gaaattctaa ttacattccc taaccttgcg cataattctg tcatgattga tttgttcaat
    13081 aatcaattgc catgggcttc ttatggtttg ctagatgaaa cccataattc tttttatact
    13141 catgatggct tccaaaaagt ttttgaaaaa gcaggattat acatcaatat tgaagattat
    13201 ttgtacttag ctgtaggtga tacagaatta aaatcaactt atgaagaatt gccggaagct
    13261 gtacgttatg atttcaaaat gcgaccattt ggcgaagtct accaatactt cttctcattg
    13321 atgaaacatc cagtagcaca aagttccatt gcgcagccgc aaaattctaa ttatgtgaaa
    13381 gtattagaag taatccagca aacgaaacaa gatgaaataa ttcaacaaat accattcaat
    13441 aatttcacag gggaaaatga gacactaagt ttccatgtat cagaattagc agaacgaatg
    13501 gtgtttcgct tcgcgcagca acctagcttt atcgaattca gcgcagaagc tgctggagaa
    13561 aaactcacat ttatcgattc aaatgctgta gtcaaaacag taaatgattg ttatttgttt
    13621 gatggtaaag atttaccaga gttcttttta acggatattg cagggaaaga agtgactatc
    13681 cattgtcact atcgttttat cggagaattg acaccaacaa tgaaagaatt gctggaaaca
    13741 atccgtccaa tggctgaagt tacaaagcag ctttctgaga aaaatgatga attagaaaga
    13801 gaaaatcatt atcttattga gaataataca agattagatc agacgttgaa aacaaccata
    13861 aatcgctatt gcacgttgtt ggaaagtgat gaatggacaa tcaaacatag attagggcga
    13921 cgtaagaaag aaacatcgaa aaaaatccaa gaaaaagagc tttctctatg tattgacgaa
    13981 aaaatctggg atgcagaaac aaagatattg aaaatcatcg ggtggggaat cgctaattcg
    14041 gatcgccaac cattgtccta taagttatca gccgaccaat caccattttt tgaagcaatt
    14101 cctgttttac gagaagaagt caatcaagca aaacagctag ctgaaggagc aaaagcagga
    14161 tttgaactgc ggatcctttg cgagaaggaa cgttcattct tagttgaagc agtggctcaa
    14221 aatggtcaat catggatcat tgaaatgtaa ataatgatta acttataaag ataaggagta
    14281 aacgaatcgt gcaaaaaaaa gaaattgccg tatttatcga tcaaatcact cgtgaacggg
    14341 ccactggcga tttgcttatt gtgggctggg caattgatga agtgaccaaa gaaatcccta
    14401 cgataaaggt agaaaaagaa aatgtgattg ctgaagcaac acatgtcgta agattggata
    14461 tcaatcattt atataatctt gatgttaaga cccaaagcgg ttttaaggta cgtttatcag
    14521 ggaaaatgag aggaaaagca atcttagact tccaaacagc aagacatcaa aatggcatag
    14581 ctgtgaaatt aaacggtaaa tatccttatg atgacggcat tgaatcttca tgggaaagaa
    14641 aaaaaagatt actgaaaaaa gggataaatt atgcaaggac tcatggcgta aaaaaagcaa
    14701 tccgccgggt caaactggaa ttgaaaccag gatcgatcga ctatgcagag tggatcagtc
    14761 gtcatgagaa acctgatctg aaagagcaaa ggaaagaagt acaaggattt gcctaccgcc
    14821 cattgatttc catacttatg cctgtctata acgttgaaat caaatggctc gaaaaatgca
    14881 ttgattcggt acttgatcaa acgtatgatc actgggaact atgtatcagt gatgatgctt
    14941 ctactgatcc agctatccga aagtgtctag aatcctatca agcaaaagat gatagaatta
    15001 aagtcgtttt ccgacaagaa aatggacaca tcagtctggc aactaattct gcattggaaa
    15061 tggcagaagg agagtttata gcgctcttag ataatgatga cgaattacct ccatttgcct
    15121 tatatgaagt ggcaaaagtg ctaaacgtac atccagaact ggatttgatc tacagtgacg
    15181 aagataaaat cgatgcagat gggaatcgct ttgacccgca cttcaaagca gactggtctc
    15241 ccgatacatt gatgggaaat aattatatct ctcacttagg cgtatatcgg acaagcattg
    15301 ttaaggaact tggcggtttc cgtaaaggat atgaaggctc tcaagattat gatttggttc
    15361 ttcgtgtgac agaacaaatt cctgcagaac acatttacca cattgaccgt gtcttgtatc
    15421 attggcggac gatcccggga tcaactgcaa gcaatggaga agctaaaagc tacatctatg
    15481 attctggagt gaaagcattg acagatgctc tatctagaag aaatatcaag ggaagcgtac
    15541 atcctggcag aatttctggt ttttatgaaa tcacttatga tgttttgcaa gaagacttgg
    15601 tcagtgtgat cattccaact aagaatggct atgaagatct aaaaacttgt gtggattcga
    15661 tcatcgaaaa aacgtcttat ccaaattatg aaatcattat tgcagataat ggaagtacag
    15721 atcctaaaat gcaagaatta tttgcagaat acaagcatca gctgaaagac cgatttatcg
    15781 tcgaattaat tgacattcca ttcaattatt cacgtatcaa caatctggca gcagaaaaag
    15841 caaacggcaa atacttttta ttcttgaata acgatacaga agtgattgaa cctgactgga
    15901 tgacagcgat ggtttcttat gcacagtttg atcgaatcgg ctgtgtagga gcaaaattat
    15961 tttatccgga tgatacaacg cagcatgcag gtgtattatt agggatcggt ggcgttgcag
    16021 gacatgcatt gaataactat gatcgaacac attgtggtta ctttggccga ttggtcattg
    16081 acgtcaatta cttagcagtc actgcagctt gtatgatggt caaggcagct gacttcaatg
    16141 ctgtcaatgg atttgatgaa atattagaag tagcattcaa cgatgttgac ttgtgcttga
    16201 aagtttatga actgggtcgt tacaatgtct atgcacatca agcagaattg taccatttcg
    16261 aatctaaatc aagaggttat gaagatacac cagaaaaaca aagacgtttt gctggggaaa
    16321 tcaaaaaaat gcaagataag tggccttctt atattgctca tgacccattt tataacgata
    16381 atctaactaa agaaggaatc ggcgattttt cgttgagacc tgattgatta aaagaactgt
    16441 attttcattt ttattaatga aaatacagtt cttttaattt aat
//
LOCUS       DAZKCY010000055        16404 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000055 DAZKCY010000000
VERSION     DAZKCY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.055"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(153..551)
                     /locus_tag="ACG8XC_001627"
     CDS             complement(153..551)
                     /locus_tag="ACG8XC_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP9191323.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="ACG8XC_001628"
     CDS             complement(548..1321)
                     /locus_tag="ACG8XC_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP9191324.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="ACG8XC_001629"
     CDS             complement(1318..2001)
                     /locus_tag="ACG8XC_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP9191325.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="ACG8XC_001630"
     CDS             complement(2168..3055)
                     /locus_tag="ACG8XC_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9191326.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="ACG8XC_001631"
     CDS             complement(3052..3921)
                     /locus_tag="ACG8XC_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9191327.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="ACG8XC_001632"
     CDS             complement(4058..5344)
                     /locus_tag="ACG8XC_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9191328.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="ACG8XC_001633"
     CDS             5617..6195
                     /locus_tag="ACG8XC_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9191329.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="ACG8XC_001634"
     CDS             6195..7124
                     /locus_tag="ACG8XC_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9191330.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="ACG8XC_001635"
     CDS             7121..8857
                     /locus_tag="ACG8XC_001635"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9191331.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACG8XC_001636"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACG8XC_001636"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFP9191332.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="ACG8XC_001637"
     CDS             complement(9753..10262)
                     /locus_tag="ACG8XC_001637"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFP9191333.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACG8XC_001638"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACG8XC_001638"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9191334.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="ACG8XC_001639"
     CDS             complement(11563..12654)
                     /locus_tag="ACG8XC_001639"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFP9191335.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACG8XC_001640"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACG8XC_001640"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP9191336.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACG8XC_001641"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACG8XC_001641"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP9191337.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACG8XC_001642"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACG8XC_001642"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFP9191338.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4737 a         3624 c         2916 g         5127 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atgcaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg atgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gaca
//
LOCUS       DAZKCY010000056        16332 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000056 DAZKCY010000000
VERSION     DAZKCY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.056"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..1018)
                     /gene="murB"
                     /locus_tag="ACG8XC_001643"
     CDS             complement(83..1018)
                     /gene="murB"
                     /locus_tag="ACG8XC_001643"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP9191339.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1091..2011)
                     /locus_tag="ACG8XC_001644"
     CDS             complement(1091..2011)
                     /locus_tag="ACG8XC_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9191340.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2124..2876
                     /locus_tag="ACG8XC_001645"
     CDS             2124..2876
                     /locus_tag="ACG8XC_001645"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP9191341.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3740..4273
                     /locus_tag="ACG8XC_001646"
     CDS             3740..4273
                     /locus_tag="ACG8XC_001646"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP9191342.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4763..5287)
                     /locus_tag="ACG8XC_001647"
     CDS             complement(4763..5287)
                     /locus_tag="ACG8XC_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFP9191343.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5280..5753)
                     /gene="tsaE"
                     /locus_tag="ACG8XC_001648"
     CDS             complement(5280..5753)
                     /gene="tsaE"
                     /locus_tag="ACG8XC_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP9191344.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5876..6856)
                     /gene="pta"
                     /locus_tag="ACG8XC_001649"
     CDS             complement(5876..6856)
                     /gene="pta"
                     /locus_tag="ACG8XC_001649"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9191345.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7009..7689)
                     /locus_tag="ACG8XC_001650"
     CDS             complement(7009..7689)
                     /locus_tag="ACG8XC_001650"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9191346.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7815..8684
                     /locus_tag="ACG8XC_001651"
     CDS             7815..8684
                     /locus_tag="ACG8XC_001651"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9191347.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8710..9225
                     /locus_tag="ACG8XC_001652"
     CDS             8710..9225
                     /locus_tag="ACG8XC_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFP9191348.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9272..9835)
                     /locus_tag="ACG8XC_001653"
     CDS             complement(9272..9835)
                     /locus_tag="ACG8XC_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9191349.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9890..10069)
                     /locus_tag="ACG8XC_001654"
     CDS             complement(9890..10069)
                     /locus_tag="ACG8XC_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191350.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10146..10655)
                     /locus_tag="ACG8XC_001655"
     CDS             complement(10146..10655)
                     /locus_tag="ACG8XC_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191351.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10751..11131)
                     /locus_tag="ACG8XC_001656"
     CDS             complement(10751..11131)
                     /locus_tag="ACG8XC_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9191352.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11461..12210
                     /gene="pnuC"
                     /locus_tag="ACG8XC_001657"
     CDS             11461..12210
                     /gene="pnuC"
                     /locus_tag="ACG8XC_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP9191353.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12394..13671)
                     /locus_tag="ACG8XC_001658"
     CDS             complement(12394..13671)
                     /locus_tag="ACG8XC_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191354.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13750..14394)
                     /gene="pcp"
                     /locus_tag="ACG8XC_001659"
     CDS             complement(13750..14394)
                     /gene="pcp"
                     /locus_tag="ACG8XC_001659"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFP9191355.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14593..15537)
                     /locus_tag="ACG8XC_001660"
     CDS             complement(14593..15537)
                     /locus_tag="ACG8XC_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFP9191356.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15539..16225)
                     /locus_tag="ACG8XC_001661"
     CDS             complement(15539..16225)
                     /locus_tag="ACG8XC_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFP9191357.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4709 a         3386 c         2883 g         5354 t
ORIGIN      
        1 gcgccatttg cgtaaacaac cacaaaaaag agcaggacgg ccaccgtcct gctcttttac
       61 ttattttaag ttccattact tattaattgg ctaaacttct gcttgtttct gatattgctt
      121 gtttttctcc aatgatccgc acttctgttt ctaaagacac atcaaatttt tctttgatta
      181 cttcttggat gtgcgcaatc aattcaatat aatctgtcgc tgtcgcatga tcgatattga
      241 cgatgaatcc agcatgtttt tcagagactt gtgcaccgcc ccatttcagg ccttgcaatc
      301 ctgcttgttg gatcagctga cctgtatagt gtcctactgg gcgcttaaag acacttccgc
      361 atgaagggta ttctaatggc tgttttgatt gacgcaattc ggtcaactca tccattcttg
      421 ctttgattgc ttcataatcc cctgtttgca aactgaatcg agcttccaat acgatagcac
      481 gtaattcttg gactttactg tgacgataag aaaataccat ttcgtctttt gtcaaagttt
      541 tcaatgttcc atcttcaagc aaaagatcca cttcagcaaa aacatctttg atctctccgc
      601 catacgcacc tgcattcata taaaccgctc caccgacact tcccgggatg ccgcaagcaa
      661 attcaaatcc agtcaatgat tctgccaaag ctgcataagt cgtatcaatc agtgaggcac
      721 ctgcttccgc aatgaccatc gttcctttga catgaatctg tttcatttca gtcagcatga
      781 tgactacacc gcgaatcccg ccatcttgaa cgatcagatt gcttgcattt cctaatacca
      841 tccaaggtat atcatggatt cggcaataat cgatgatttg ctttacttct tcttgttttt
      901 tcgggaatgc aaggacatca gctggtccac ctgttttcgt aaacgtatag ttcatcaatg
      961 gttcatcaaa caatatagta atcgtggcta agctttcaac tatttcttct ttattcactg
     1021 aggattttcc tttcttttct caaacgtcct cattattcta tcacgcttaa tacaatcttg
     1081 ccagtcttgt tcaaagttga tgacggtagt gatctttaag cacttcctca cataattcat
     1141 gacttcttac aacttctttt tgtttaagca attctgtttc cgatttacct aaacggatct
     1201 gagaaaagaa ggcctctacc attggctcaa acccgcgttt gactagcgtg tttgtccagt
     1261 cgccaaattc tttcactttt tttccatcag catcttgaat gacaagctgg gtcagatcgt
     1321 ttacttcata agtaccattt ttacttgtta cttgatattt ttctatatta gcgccacttg
     1381 ctaaatccat cgttaaaata gccgtttgat tttgtgtttc taattgtaaa atggcatatt
     1441 ccaaaaattc ctttgtttct cttatctggt attttgttct caaaatgggt tcatctaata
     1501 aatacacagc tgtatcaata agatgtaaaa acaaatcata aaggacgaac tctgtcgttt
     1561 cttttgccgc tattcgattt ttttgcaggt ggatcaaccg cttatctggg atacgtttca
     1621 attcttctac cataggagca aagcgccggt tgaaaccgat catcaaaatt atttggttct
     1681 tatcagctaa ttcttgcaat tcacgaactt catttagatg gacactcagt ggtttgtcaa
     1741 tataaacatg gataccattt tctagacatt ttttggctaa ttcataatgt acttttgttg
     1801 ctgcgtggat catacatgct tcgatttttt cttctaaaag ctcatcgatc gtttcatata
     1861 gatgttggaa cccgtactct ttttgaattc tttcttttgt ttctttattt cttgttgcaa
     1921 aataaaaatc aagtgttcct tgttttttcg cataagttgg taaatatgct ttttctgcga
     1981 tattccctac accgattaca ccaatcttca tctactttcc tcctggatca tttagttact
     2041 ttcatcttac accatttttt actaaaatga ggtacaatag tatcgcaaag gtaaggaaat
     2101 taattcgaag gagtgtgctt ttattgaaat ttatctcttg gaacgtaaac gggcttcgtg
     2161 ccatcgtaaa taaaaatttt ttagaagttt ttcatgagtt agacgccgat tttttctgtc
     2221 tgcaggaaac gaaactccaa gcaggacaaa ttgatttaga tcttccaggt tattatcaat
     2281 attggaatta cgcggaacga aaaggctatt ccggcacagc gatttttgct aaaaaaccag
     2341 cattgaatgc cacttacgga atgggtatcg atatccatga tacagaaggc cgtctgatta
     2401 ctttggaata ttccgatttc tttcttgtaa cctgttacac gccaaattct caaagcgaat
     2461 taaaacgttt agattaccga ttagaatggg aagaagcctt ttataattat ttggaaaatc
     2521 taaaaaaaca aaaaccagtc atcgtgtgtg gggatctaaa tgtcgctcat caaaagattg
     2581 atttgaaaaa ctggaaaacg aatcaaaaaa atgctggatt cactccagaa gaaagagcgg
     2641 ctctttctcg actgttggat aacgggttta tcgatacatt ccgttatttt tatccaaccc
     2701 aggaaggtgt ctactcttgg tggaactacc gattcaattc aaggaaaaac aatgctggtt
     2761 ggcggatcga ttactttttg acgagcaaag atttagagcc aggactagct gatgcaaaga
     2821 tccatacgga cattatgggc agtgatcatt gtccagtaga attagatttg aaataaagag
     2881 gttgtgaaaa agtcgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
     2941 cattttgcat aaatttggca taataccgag aaactggctt ttgaacactg tttatcgaga
     3001 gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
     3061 atatttttcg tttggttggg cttattaccg cagatacaag cttttgaaca ccgtttatta
     3121 agaggttgtg aaaaagctgc actgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3181 ctcatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta
     3241 ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag
     3301 ttcctcatat ttttcgtttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt
     3361 ttattgagaa gttgtgaaat cagcgtcctg acctgagtat tagagaaaca aatggcaaaa
     3421 tagttcctca tattttgcta ttttttgagc taataccgag ggtcagctga ttgaacaccg
     3481 tttagtggag aagttgtgaa aaagtcgcct actttcgagt cgtaagaaat ttttttgaaa
     3541 atggttcctc acaactttgc ataaatttga cttaataccg agaagtcatg atccccatct
     3601 aactccaaat cacaaggaat gagttcaaaa actagctttt ctcaacaaat attacataaa
     3661 aaattctatg ttttttgtta aatttatttg cttttttcaa aagtatgtta cagtaatttc
     3721 ccaaggaggt tttacagaaa tgaattttgt tgccatggat tttgaaactg ccaatcatca
     3781 gccttatagt gcttgttctc ttgcgctagt catggtaaaa aatagccaga tcgttgatga
     3841 attctataca ttgatacagc ctgagactcc ttttttctgg cgaaatgtcc aaattcacgg
     3901 aatccatcaa gaagacgtac gcaatgcgcc gaaattccca gaagtctggc aaactatcca
     3961 accatatttt caaaaaaatc gtttagtcgt tgcacataat gctgcttttg atacaaaggt
     4021 cttagcggga tgcttagatt attatcatct tgaacagcct aattatcttt ctttatgtac
     4081 agtgaaaagt agccgtcgcc tgtttcctga atttccaaat catcgattga acactgtgtg
     4141 cgaaaatttg aacatccaat tagatcatca ccatgatgca ttggaagata gtcgtgcatg
     4201 tgcggaaatc ctgctttatc aagaaaaaaa attcgggaca gatccattga aaaaacttgt
     4261 gactataaag taaaagaata cttatgaaac acacaaagag gtcgtgagcc tgccgtttca
     4321 ctccgagtca caaggagcaa tttcaaaaaa tagcttctca tgtttattaa atattgtgac
     4381 tggtgaccga ggagctggca ggagaacacc gtttattgga gaggttgtga aaaagctgtt
     4441 ctgcatcaag taataagaac agccaaacaa aaatagcttc tcatattttt catttggttg
     4501 gacttattac cgcagatgca agcttttgga caccgtttac caagaggttg tgaaatcagc
     4561 gttttgacct gagtattaga gaaacaaacg gcaaaatagt tcctcatatt ttgccgtttt
     4621 ttgagctaat accgaaggtc agctgattga acaccgttta ttcaaaaaaa atggactgcg
     4681 acacgacttt tgtcacagtc ccttcgtgtg tttttctgcc ttctcaaaag gatgacagtt
     4741 tctcactctt ttcctatttc ttctattcaa tcaaataact cattttatat acatctaaaa
     4801 attcacctag atctgttctt gcccctctag gcatgaccgc ttcgatttgg aagcccattt
     4861 ttcggtaaag gtgtacggct ctttcattac gaacttgaac gtcaagctcc aatcgaaaaa
     4921 gtacacccat ttccttcgcc caactgatga tttcttctag cataagagtg cctagtccca
     4981 ttccccaata ttcctgcaga atactgatac cgacttctcc cacgtgagac aaacgaaatt
     5041 gactgtctgc cttgacagat gctgttccaa taatcgtccc ttcatagatt gcaagcagca
     5101 ataaattatt attgctttcg tatatataat ccaactcttc ttctaaggtt tctggtggta
     5161 acaggagttc tgcttcatcc aaaactaaaa attctgtctc cttattaact tgtcccatca
     5221 tcaaaagaat gtcttttgca tctcctggca ctgcttcccg caagatgatc ccaacttctt
     5281 cactcatgat tttcagccca tttttctaga attcgctgtt tgaaggtttc actttgctcc
     5341 ccgtatgaga ttagtttcac ataccgcttt tctaaatctg tatcactttt ttctaaaatc
     5401 agttccaaat aatcctccgg cagtgcttct tctaatagat ttccccattc aacaacggaa
     5461 agcccatcac cttcaaaata ttcatctaac cctaaatctg ctccgctagc tgctacccga
     5521 taaatatcca tatgatacaa cggaatccga ccttgactat attctctaat aatcgtgtat
     5581 gtcggacttt tgatcatttg cgttatccct aatccttggg caattccttt cgtcaacgtt
     5641 gttttacccg cacctagatc accagtcaat atcagattgt cacctggcat tgcggaatga
     5701 ccaattgcct ctgcgaattg ttctgtcatt gttaatcctg atagttcgat catttttcat
     5761 cttctcccgc tcttctttct ttttgccagt atatcgcgta atgaagtttt tttaaagaaa
     5821 aaatgaacta gtcaagaaca gcagctgctg tttttggcta gttcgcgtgt atttgttaat
     5881 caatcaatgt ttgtgctgct gtgataatcg ataatttata tacatcttct tcatttgctc
     5941 cacgagacaa atcagaaacc ggtttgttca agccttgtag aattggtccg attgcttcaa
     6001 agttaccgaa acgctgagca attttatagc cgatatttcc tgactgcaat tctggaaata
     6061 caaagactgt tgcttgacca gctacttctg aatctggcgc tttcagttgt gcaactgaag
     6121 gaacataaga agcatcaaat tgcaattcgc cgtcgatcat gtattgtggc gctagttttt
     6181 tagcgatttt agttgcttca actactttgt ctacttctgg cgatttcgct gagccttttg
     6241 tcgagaagct catcattgct acttttggtt cgatatcgaa caattctgct gtttttgcag
     6301 aatctacggc gatttcagcc aattcttgcg cagttggatt cacgttgatc gcacaatccg
     6361 aaaagacata tttttcttga tctcttccac gtaccatgat catcgctccg cttgtccgac
     6421 tgatgccagg ctttgttttg atgatctgaa gtgctgggcg aacagtgtcg ccagtcgaat
     6481 ggatcgctcc agagaccatt ccgtctgcaa tccccatata cgtaagcatc gtaccaaagt
     6541 aattcacatc ttttaaaatc ttttctgctt gttctttgga tgcctttcca tttctacgtt
     6601 caacaaaagt atccaccatt tcatcccact tatcgtaatt atcagggctg atgatcgtaa
     6661 agctagatgt tttgattcct ctggcatgcg ctgcatttct tatttcttct tcgttcccta
     6721 ctaaaatggg ttccacaagt tcttctgctt tcaaccgggc agctgcgcct aaaatacgag
     6781 catcagtagc ttccgggaaa acgattttga tcccacggcg aacaatttta aatcttaaac
     6841 tatcaaacaa ttccacaaga tgacctccag acgtttttat atgtattatc atacactatt
     6901 ccagaaaaaa agaacagttt attttatctg tatcagtcaa aaatgctgga aaaattaagt
     6961 ttttcacata caaagcgaac tgcactataa caggaaagcg ctgttttatt acactgtttc
     7021 aggcagttgc caatcaatcg gttcttctcc taacgcaagg agcgcatcat tcgttttcga
     7081 gaagggtttg gaaccaaaaa agccacgact tgcagataac ggactgggat gtggagatgt
     7141 tatgatgata tggcgactct tatcgatcat tttgattttt tcttgtgccg gttttcccca
     7201 caagataaac acgattggtt tttcccgttc atttaatttt tcaatgatcg tgtctgttaa
     7261 tctttcccaa cctttcccgc gatgagaata cgcttgccct tcccgaacgg taagaacggt
     7321 gttgagtaaa aggactccct gtttagccca tgacactaga tttccatgtt gaacaggtgc
     7381 gattccaaga tcatcataaa gctctttata gatatttctt aacgatggcg ggatctttac
     7441 gccaggttgc acagaaaaag ataagccatg agcttgattt tctccatgat aaggatcttg
     7501 tcctaaaatc accactttaa cttcttcata aggagtcaat tccaatgctt catagatatg
     7561 atacatatct ggatggatct tttgggacgc atattctttt tttaaaaatt ctcttagatg
     7621 cagataataa tcttttgaaa attctgagga caaaacttct tgccaactgt tatgaatgat
     7681 ttctttcaaa aactccacct cctaataaaa agaataactc acattttctt caatcacaag
     7741 ggacaatctt caccctcagc acttgattaa tatggtaaac taggaacaag aagcagaaag
     7801 gaacgataat cattatgatc aaattaattg catcagatat ggacggcaca ttattagatg
     7861 cccatatgag tgtttctcct gaaaatgccg aggcgatacg ttttgcaaat gatgccggtg
     7921 ttgagttcat ggtcgctact ggtcgaaact atcaagaagc tcgcgcagct ttagatgagg
     7981 taggaatcga ttgtgcgatg atcactttga acggggcaca ggtatttgat aaagaaggaa
     8041 attcattgtt taccgtgtcg attccaaata atcaagcaat ctctgtttta gatattctag
     8101 atgctaacgg tatttactat gaagtagcaa ccaacgatgg tctttattct gaaaatcaag
     8161 cgaaacgaat tgaaagtttt gcttccatgg tggctaccca tttacctcac cttacgtata
     8221 aaatggcgat tgccatggcc tcagctaagc tggagttatt gcacatcaca tacgtggaca
     8281 gtattcgtgc tattttggaa gaaaagaatc tggaagtttt aaaaattatt tgttttcata
     8341 cagaagggcc agctgtatta ggtccagttg gaaagcagat ttctctttta ggcgatctag
     8401 ccgttacttc ctctggtcaa aacaatatcg aagtgaacca taaaaacgct caaaaaggaa
     8461 ttgctgttgc ccatgtcgct cacgagcgtg ggatttcttt agaagaagtc atgacgattg
     8521 gcgataactt caatgatgtc agcatgctgc aagtagcagg agtcagtttc gcaatgggaa
     8581 atgcagaaat cgaagtaaaa gattatgcca aatatacaac ggacacaaac cttgaatctg
     8641 gcgtaggaaa agccatattg cgcgcgattg cagaagactt gtagttttat ctagaaaaga
     8701 gggatataga tgtcagaatt tttcattcag gaaaagcagc ttggacgtgc aactcggaca
     8761 attgtcaaaa acgaaaaagg acattccctg tttttgatgg ttggtcgctg gggaacaaag
     8821 ggcgatgcat tgtctcttta tgccatgaat ggcgatctcg ttgctcacat caagcagata
     8881 agcctgacat tcggtacgcg ttttgagctg tataaagagt tcgaaaaggt gggaaccatg
     8941 cagaaaatat tcaattggcc cggtgacttt tattatattc gtagacttca ctggacagtc
     9001 caaggtgata tctataatca tcaatatcaa atccatcatt tcaatcagca aatcatgaaa
     9061 atggataaag caacactttt ttcaggagat tactatgtat tggatgtctc aaatgatgaa
     9121 gatgctccag tttgtatttg tattgctgcg gttttagatt actggctcta ccgtaaagac
     9181 aaaagtcgcg ataagcctgt ttcttaccga cttaacttaa gttaaaaaga tgtacctgaa
     9241 atgcggaaat acgcatttca ggtacatctt tttagatatt cttttgcata tgatcataaa
     9301 gatgaccact gatcatccgc tgaccgacaa ctttgaagtc atggcgttcg tataattttt
     9361 tagcctttgg atttttttca tccacactta gaccgatgac ggagcgatta gctttcttcg
     9421 ccaatttagg taatgcttct aataatcgcg aaccgatgcc ttgtcctcta aaatcttctg
     9481 atacactgat agagtccaaa taccattcgt tgggaaacgt ttctttatca atgaacaaac
     9541 gtgtagacgc atctagctga tatttctcta agataggtac cagtggctga tcgatgattg
     9601 attcttcttc atctgtataa ccgtaagcaa ctcctgcaac tttaccgtct atttcttcta
     9661 cgatacctcg tgaataactg taacgataat ttggatcagt gattgcttct tctaaaatct
     9721 ctaaagtctt ttgttcccct gtctgcttca aaaatggcag ttccatatct tttagaatca
     9781 ctaagatcaa tggggcgatt tctggagcat cttcttttct cgcaaaacga atcatagttt
     9841 cctctccttt tctccattct aacatgcaga aaaagaaatc gcaaaatcgt tattttaaaa
     9901 aatcttcaat cttttcagta atgaaataac catgagtaac gattcttgcc gcttcagttc
     9961 ggcaataagg gcagacgact ggcatttctt tatcattatg ttcgactaca ttattgactt
    10021 cttcgtatgc aaattttttt ccgcaattag ggcatttcat tttttccatg ttatctcaac
    10081 ttcctttcat ctttactctc ttagcttact cttatattgt aaaaaagaga agcaatatcc
    10141 ttcttctatg cagatatcca tacctgacgg tcctgttctc catctattaa tcggacttta
    10201 taataaggac gttcatcttt gactagaagt gtccactctt tcacttttgc ttttttcccg
    10261 tagtagctga ctgcttcctt catcgctgct tgcggttgtt tgaactcttc cgttttttct
    10321 atatcaaaaa gatgttctgc ggccttttcc gtgtcatttc gtacggttgt tttcccagtg
    10381 gatggattga ctgctatgac ttgtccttca caaaaaaact ggtattcata tgttgcattc
    10441 gtaggattct ccaaatcttt ttcaactgca aaatgaatag cagatatgtt atgttctttt
    10501 gtttcgtttt ttacgattgc tgctgctttt ttcaatgaga tccgatattc ctggtcacta
    10561 atatcatcca tgtgaaaagc ggcattgagc agtcccgtca taccggtaat ccctattacg
    10621 atgagggcag caacaccaaa catcattttt ttcattcttc attctcctgt ttcttttttt
    10681 taaagaatat caagaaatga acgaatgaaa aatcgaatga aacgaaaaaa aagtgacaat
    10741 tttgttatct ttattcgtgc agttctaatg gcaatccgtc tggatcaaag aaaaacgtca
    10801 ttttttttcc tgtaaaggta tcttctctaa tcggttctga ttcgatccct aaggactgca
    10861 attctgcaat tgtttcttca atatcgtcta ctttgaaagc aagatgacgc aagccacaag
    10921 cttctggata gtttggtcgt tgtggcgcat tagcaattcc aaaaatctcc aattcagcac
    10981 ttcctagttt cagatctaat ttatagtcat ctcgttcttt gcggtaattt tctctgatta
    11041 cttcaaatcc taatttatta acataaaaat cacgtgattt ctgataatct gaaacaatga
    11101 tggctatatg atggatcgtt gtaaggttca tatgagcact cctattcttc gtttaagctc
    11161 ggatctatat taatctccag ttccattgta aatgtgtcag attgaaaaaa atagctgttt
    11221 ttccttttta taagaaaaaa ggcaaaaaaa acagaaagta ggtcctccaa gacactcttc
    11281 ctgttcccaa tataataaat gaaagtaaga aattatattt tcaagaaaac aaacagaaaa
    11341 ttatcataac acaaattatt tataattgaa agactagttt tttttcaaga aaagcataca
    11401 atagattgtg ttagtaaata cagaacacac tacatttagt gtcttaagga gtcctcatac
    11461 atgaagtcat attttcagtt tttagctcat cagctaaaag gctggccgca acaaaattat
    11521 tatcttttct ttttcagtct tggctgccag gtcatgactc ttgtcaatag tcccatcact
    11581 tggctgtccg ttattacatt tatcggtacc acgcttggtg tattgtgtat tttatccatc
    11641 aatgcggcaa aatcagtcaa cggtatttta ggtattttgt ctgcgatttg ttttattatc
    11701 gttgggtttt cagcaaagaa ctatttaagt atcggtgaac agattgctta tatgatcaca
    11761 ttggatatcc ccgtattgtt aagtaaagaa tggaatcata atatggcttc aaaaatccga
    11821 aaattcactg gaaaaagctg gtttatcgca atcatcagta ctttccttgt ctatcttgtt
    11881 tcaggattcc tgattggtcg ttatacagat gatcctcggc cgtgggtaga tgccattgct
    11941 ttttctatta gcttgactgc tggtatcatc agtttcttgc gttacaacaa ccaatattac
    12001 tggtggttag cttcagggct tgctcaaatg gtcctttggt atatttcatt tacgcatgga
    12061 tctgcaagtt tggcgatgtt cgtgaacagc tctgtttact tgctgaatga tgtccttgca
    12121 tttaccgttt caccttggta taaccaaaaa gaacgtgaac gtatgcaaaa aatggaagaa
    12181 gaatactaca acgcacaaca acacgcataa aaaaactgag cgtgggacaa aagtaaagaa
    12241 cacttttgtc tcacgctcta aacacgtata aacggcggaa tcagaagcaa ctccttcgga
    12301 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt
    12361 ggagttaaac gcttctgtcc cagcctctat tttttatagt aattccttca gatactttct
    12421 tgcctcatca ttgaaacgca gtgattcttc taacttctga taatattcca acgcttgttt
    12481 ctgagcttca gtaaaacgat aatgttttcc tttccatttg tcgattgtct cttgcacatt
    12541 tccagattgg atatctttca ctactagttg ccatttttct tttgagactg tacggtcttt
    12601 cttatcagca gattgtatag cttctcgttc tttacgcaat acttttaaaa aaccatttct
    12661 gactatccga ggaatgctgt cgaagaaccc ttctgtaaaa ccatagcgac aatattgctt
    12721 ccaaataaat agatagattt ttggttggat gaatcgttgg tttttcgcaa aatattccat
    12781 ctgagcagtg acttctggtg ttcgttgacg ttttgtcttt ctcaacacgg aaataaatgt
    12841 ttcaaaataa gcattaggat gtactggcac ttcttcatat acgatggatt gccaatcttt
    12901 acgtaaaaat gcgcgatagt tgatcaagaa ttcttctaca tcctttacta attcttctgc
    12961 aaagtcgtca aatagttctt tttttatctg tcgctttttt gactgcccat tgatgtgttc
    13021 acttattttc ttgatttcat tttccaatgc ttgataatcc gctatgtaaa tcggcatttc
    13081 tacttggcga aattctgcga gccgctgagc taattttata tccggtaaat atggctgttt
    13141 catccgacga ttttggtaca agacaaaaca gtatttttgc catagtcctt caggaaaatc
    13201 gatattcttc tgcttaagcc ataataattc gtctaaggcc agatcaactt ttgtcattcc
    13261 ctgatggatc gaagctgcca ctgtcggaga gacatgcagg tgatcggcaa agtggttgat
    13321 gcctagtttt aggatctttc cggtttttgg cgatttaacg atgtattgca cttttaattc
    13381 tctaccacac tcacaataca atttttgctg gcttcgtttt gttcgaaaaa acggattgat
    13441 cttgaagtca gaaaacaccc aatctgttgc agctaaataa tgttcttcca taaaacgaga
    13501 gctgattaga tatcggcggt gcttatcgac tagttcccgc tgttttttcg ttaattttgc
    13561 atagatctcc caacgttgac tgcttgtgta ttgtttagaa attttgatgg gatagctttt
    13621 gctgatttct tttggaaacc attcttcaaa gacggattgc cattcttcca cggctgtttc
    13681 tttcctttct aattacaaaa agctggctac taacataacg ttagcaacca gccagtgttt
    13741 cttgctaaat tagtgagttg ttccggcatt tccggaaata tcttctttat tttctactgc
    13801 tgcttcaact gcataagtca aagaagaagc aatgtcttct aaagacatag aaggcgtacc
    13861 cactggacga tcgatcactt gatcgggtga gaatggcacg tggatgaacc cacctcgaat
    13921 tgccgggaat tgtgtttcga tcaaatgaag caaacgatac atgatatcat tgcagacaaa
    13981 tgttcctgct gtataagaga tgtacgctgg tagaccatga tcttgtacat tttttgtcat
    14041 tgctttgatt ggcaatgagg taaagtaagc ggctggaccg ttttcttcta aagccgtatc
    14101 gaatggctga tttccttcat tatcagggat tcttgcttct gctagattga ttgcgacacg
    14161 ttcgaacgag actgctgacc gcccacctgc ttgtccgaca cagatcacga catctggttg
    14221 gtgctgttgg atggcttctt tgactacttg actgcttttt tcgaatacgg tcggtacttc
    14281 cactttgatg atttcagcac cgctgatttc atctggcatt ttttttactg cttcataggc
    14341 tggattcact ttgtccccac caaatggatc aaaacctgtt actagtactt tcatctgttg
    14401 ttcctccgtc cgattgattt aacatatggt tatcttttat cataccattt tccttgaaca
    14461 gcgtaaataa aggaaacaat agtttaaata acataacaga tcaaacttaa tatattctta
    14521 taaaaaaata gtgttccact acaatgttag aagaggttgc gacaattatt ctgtcacaac
    14581 ccctctcttc tattatttaa atagaatcat ataagcaatt tggaaaacta gcataaataa
    14641 cgcaataaac aactggtttt tgatcactcc gtatttgtct ttcatctcca gcatcgctac
    14701 aggcacgata ttgaagttag cagccatcgg tgtcagcagg gtcccacaaa aaccgcaagt
    14761 caatgcaacc attcctatta acgctggatt cgctccatga ctaaatacga atggtgcacc
    14821 gatcccaacc gtcatcacgg tgatggccgc aaatgcattc cccatgatta ccgtaaatat
    14881 caccatgcca agagcgtaaa tgatgatgcc gatgttcaca tttccttctg gaacgatcgt
    14941 acctactgca ttggaaatca ctgtcccaac tccagcgctt gtaaataccg cacctagaga
    15001 agctaaaagc attggcaaca tgcttaacgg ccctactgtg cccagcatat ctgctgcatc
    15061 atctaaaaat gtacttggtc gattatctcg agaaaagaac atcaagatta gcatggctgc
    15121 aaaaacaccg acgcccacgc cgactaaagc acctaaattg gtaaatagag caaacaagat
    15181 cgcaaagaca ccgatactca aagctggaat aaaaattttc attcctagtt tgtctgacat
    15241 tttttccata tattcttttg atggaagccg gctttctcct ttgctgactt tcttagcaat
    15301 tgctggtatg gtcatcgcaa agatcaagcc accgttcacg attgccggaa gccatcttcc
    15361 tcctgcaata actaccccta agaccgtcca gaaaaaggct gacccataac gatgaggatt
    15421 ggttttatct gttaaatttc ttactccagt gtaaatcaat acgagtccca tcacgatata
    15481 aaggatctcc agtaatttat cgcctaataa aatatcagga tttgtaaaaa agtccatctt
    15541 attgcccctt tcctggataa tatttcttcg ctaatttctt ttctttaaga tagaagtaga
    15601 cacttgccac aactaaagcc gtgatagcca ctggaacttc cactaaagca agctgtccca
    15661 atgttacgtc atatcctaga tctttcagtg ttgattggac tagcaacgcc cctgctcctc
    15721 cgatgaatag tacttggcca aagaaccagc agacattttc catagctgaa gccatacctt
    15781 ttagttcttc ttcatgctta ggatggactt ctaacccttt tgattcgatg gtccccaatg
    15841 ccatcggtaa aatgattgga cgaacaaatc cagcaactcc accaaaactc acgttaaaag
    15901 ccgcaaagat cattcgaaag atcccataaa tatctacaat cataccagca gatactttct
    15961 tgaatcgctt gatcaaggaa gccgctgatt ctttcagacc atttctttca agcgttcctg
    16021 ttgccaacat aatgatgata aaaatcgcca tcccgcgatt gtttacaaaa cttgtaccta
    16081 aaagttctaa cattccttga atgccaagac cgccagtcaa agctgtaaca actaaagcaa
    16141 ccattacgat caatatcgag tctaatttaa atgcaaaccc gagaataaca atcaagacgc
    16201 ctaaaaggct aagataattt tccatatatt ttcctccata aaatgacaat atttttttaa
    16261 ttataaagca aaacacataa aaaatatagt tgttttctca cgaaatagac actattttta
    16321 aatatttttc gt
//
LOCUS       DAZKCY010000057        16277 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000057 DAZKCY010000000
VERSION     DAZKCY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.057"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..636)
                     /locus_tag="ACG8XC_001662"
     CDS             complement(<1..636)
                     /locus_tag="ACG8XC_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191358.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDL"
     gene            complement(815..1534)
                     /locus_tag="ACG8XC_001663"
     CDS             complement(815..1534)
                     /locus_tag="ACG8XC_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9191359.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1665..2816)
                     /locus_tag="ACG8XC_001664"
     CDS             complement(1665..2816)
                     /locus_tag="ACG8XC_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFP9191360.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3033..3773)
                     /locus_tag="ACG8XC_001665"
     CDS             complement(3033..3773)
                     /locus_tag="ACG8XC_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9191361.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3777..4100)
                     /gene="rsfS"
                     /locus_tag="ACG8XC_001666"
     CDS             complement(3777..4100)
                     /gene="rsfS"
                     /locus_tag="ACG8XC_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP9191362.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4157..4750)
                     /gene="yqeK"
                     /locus_tag="ACG8XC_001667"
     CDS             complement(4157..4750)
                     /gene="yqeK"
                     /locus_tag="ACG8XC_001667"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFP9191363.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4734..5384)
                     /locus_tag="ACG8XC_001668"
     CDS             complement(4734..5384)
                     /locus_tag="ACG8XC_001668"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP9191364.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5394..5705)
                     /gene="yhbY"
                     /locus_tag="ACG8XC_001669"
     CDS             complement(5394..5705)
                     /gene="yhbY"
                     /locus_tag="ACG8XC_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP9191365.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5721..6830)
                     /gene="yqeH"
                     /locus_tag="ACG8XC_001670"
     CDS             complement(5721..6830)
                     /gene="yqeH"
                     /locus_tag="ACG8XC_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFP9191366.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6827..7354)
                     /locus_tag="ACG8XC_001671"
     CDS             complement(6827..7354)
                     /locus_tag="ACG8XC_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFP9191367.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7534..8319)
                     /locus_tag="ACG8XC_001672"
     CDS             complement(7534..8319)
                     /locus_tag="ACG8XC_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP9191368.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8312..9181)
                     /gene="accD"
                     /locus_tag="ACG8XC_001673"
     CDS             complement(8312..9181)
                     /gene="accD"
                     /locus_tag="ACG8XC_001673"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP9191369.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9182..10561)
                     /locus_tag="ACG8XC_001674"
     CDS             complement(9182..10561)
                     /locus_tag="ACG8XC_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP9191370.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10569..10988)
                     /gene="fabZ"
                     /locus_tag="ACG8XC_001675"
     CDS             complement(10569..10988)
                     /gene="fabZ"
                     /locus_tag="ACG8XC_001675"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9191371.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11013..11489)
                     /gene="accB"
                     /locus_tag="ACG8XC_001676"
     CDS             complement(11013..11489)
                     /gene="accB"
                     /locus_tag="ACG8XC_001676"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP9191372.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11492..12727)
                     /gene="fabF"
                     /locus_tag="ACG8XC_001677"
     CDS             complement(11492..12727)
                     /gene="fabF"
                     /locus_tag="ACG8XC_001677"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP9191373.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12746..13483)
                     /gene="fabG"
                     /locus_tag="ACG8XC_001678"
     CDS             complement(12746..13483)
                     /gene="fabG"
                     /locus_tag="ACG8XC_001678"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFP9191374.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13495..14412)
                     /gene="fabD"
                     /locus_tag="ACG8XC_001679"
     CDS             complement(13495..14412)
                     /gene="fabD"
                     /locus_tag="ACG8XC_001679"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9191375.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14519..14743)
                     /locus_tag="ACG8XC_001680"
     CDS             complement(14519..14743)
                     /locus_tag="ACG8XC_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9191376.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14796..15758)
                     /locus_tag="ACG8XC_001681"
     CDS             complement(14796..15758)
                     /locus_tag="ACG8XC_001681"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP9191377.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15768..16208)
                     /locus_tag="ACG8XC_001682"
     CDS             complement(15768..16208)
                     /locus_tag="ACG8XC_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9191378.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4288 a         3713 c         2785 g         5491 t
ORIGIN      
        1 taaatccaaa tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc
       61 ttccatgttt tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt
      121 gtcgatcaat cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct
      181 gattggatag ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc
      241 gatcacttta ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg
      301 acgatgtttc aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc
      361 aaataaccga aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct
      421 ataaatcagc attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt
      481 accgacatag tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa
      541 ttttgaacaa tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat
      601 acgttcaaaa ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca
      661 tacttcatat catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa
      721 agaaaataaa tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga
      781 aaaagactga atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga
      841 acacatgctg tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt
      901 gatcttctgg agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa
      961 aatcagtgat tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac
     1021 cgtaaacgat gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca
     1081 ttaggtattc aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt
     1141 caaacatgta agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg
     1201 cccgtacgtc tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg
     1261 agccgcttgg tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt
     1321 ttgctttttc gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat
     1381 caatgacgaa acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg
     1441 cgtagatttc aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt
     1501 tctttgctac gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat
     1561 agtttcataa cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc
     1621 tttttttctt tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg
     1681 atttgcccga aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta
     1741 actaacagtt ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct
     1801 tttttttctt ttaagaatct tcttccagca tcggtaaatc caagtagtcg aagataattt
     1861 ttattttgtt ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat
     1921 cgttggatcc gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa
     1981 ctatcagcag ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag
     2041 atatctgtca gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa
     2101 gattcccaag tctgcagcga acaagttgaa agatcttctt ctgtctgagg cggcacaagt
     2161 ccagctattc tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc
     2221 tgatctgtga tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc
     2281 atagggtgga catacttggc attttccttt gcataactta atcccaagat atgatttggc
     2341 gaagaaaaat caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc
     2401 ggataactat acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc
     2461 tcttggacaa agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa
     2521 cacaaactgt cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca
     2581 gctgactgaa ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa
     2641 gcttcattcg cgcgatgcca tttatcgatg atagccggtt cgcctctttg caaaaaattc
     2701 ccgctcatta cagcaatgac gacctctgct cctgttattt ttcgtgccat ttccacatga
     2761 tagcgatgtc cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc
     2821 atcctccttc atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc
     2881 ggaaaaaata cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa
     2941 gctgcactgc aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat
     3001 tcttttcttg ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag
     3061 cgctttgatt tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa
     3121 aagccgctgt tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt
     3181 agttcatctt cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga
     3241 aaatgttcga tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta
     3301 tccccttgat aatgatagct atattctgga aaaacctcat ctacttgata ggtggagtgt
     3361 acgtcaaata aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc
     3421 tgtacctctt gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga
     3481 tattgtccga tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct
     3541 tcttcatatg ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc
     3601 actttatatc catcttttgc aaaggcaacc gctaatgctc cagtaccgca tgccagttcc
     3661 aaaatatttt ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa
     3721 agtgtttcgt ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa
     3781 tccaaccatt cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag
     3841 aaatttcgtt ctggcgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc
     3901 cattttccgc cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta
     3961 tcaatgattt cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg
     4021 aaataatctg ccaacaagga aacatttcga acgtctaaag cgataatgtc ttctgcccgt
     4081 tttgaatctg ctgcttcaat cgcaattttt aattgttctt tactatctat gatgatttcc
     4141 tcctaatatg agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg
     4201 gataaatctg ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta
     4261 cagcttcgtc tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc
     4321 gacctggttc aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag
     4381 ccccagtcgt atgcacccgg attgcatgaa gaatctctgc atcggtgatt cctaattccc
     4441 gctctacaaa cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact
     4501 ccggatcaaa accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg
     4561 cataatcatg tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg
     4621 caagagccac ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc
     4681 tcatctgtat tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt
     4741 ccatattcat acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag
     4801 cggatagaac agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca
     4861 ggtacatcta cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata
     4921 cccacaaagt tgaccataga agtcagctca tcgattttat accattttgg caaatattct
     4981 accatatccc caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc
     5041 ttcatagtat cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc
     5101 aaaaaaggat tgtcttccac cgctaattcc aacatgttga gtcgatgttt tgcatcaatc
     5161 gtctgtttct catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga
     5221 cctaaattcc ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat
     5281 gttccgccca agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga
     5341 aataattgtg aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt
     5401 gcttttactt cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca
     5461 ataacacgtc caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct
     5521 gcttcttctg caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt
     5581 tctaaagctt cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga
     5641 aaaatcggct gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa
     5701 ctcataattt tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt
     5761 ggcgcccagc ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa
     5821 acgatgtctg ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg
     5881 acttcatcag gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct
     5941 gctttttcca gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa
     6001 gcacgattgc cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct
     6061 tcattcagct gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct
     6121 tttttaccta agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa
     6181 aaatgcccgt catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa
     6241 gttgtgatta catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat
     6301 tttccgacat ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt
     6361 ttttctaata gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact
     6421 ggacgaattc ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc
     6481 aatgatttag gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca
     6541 aaacggtgta gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg
     6601 atcaatgcat ctttttgccc taattcattc agtaagcgca aaaagtcgtc atccgtcaat
     6661 gagacatctt ggatctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc
     6721 ccggaagcta aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg
     6781 tttgtttgta taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac
     6841 ctcgccagcc catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa
     6901 aacgattaaa ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac
     6961 gtatccctgc acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta
     7021 tctcatcatc tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt
     7081 tcattgctcg tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg
     7141 aattattgtt tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag
     7201 taagcaactc ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg
     7261 tcaatactgc tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt
     7321 ctaccatcca tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga
     7381 aggagcacat aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac
     7441 ctctatactc gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga
     7501 ttgatcacag aaaaatttag aaacaaaaaa caactaatat tggcggaacc tcttgtatcg
     7561 tttttccagt aatttatgct gatctaatcg aacaagttcc gtcaatcttt caatcaacgc
     7621 ttttttcata atgtgtgcga ctttatatcg tggcaactgc tcgccgttga acgtttcagg
     7681 aataactcga tcaacgattc ctaactcgcg tagttctggc gcagtaattt tcatcaactc
     7741 agctgcttct gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc cttctggaga
     7801 aagcacagca taaatcgttt cttccagcat ccagacttca tccgctaaac ctagagcaag
     7861 ggcaccgcca cttccacctt caccaatgat gatggcgatg atcggtacct ttaaatcaga
     7921 catttcgagc agatttctgg caatagcctc tccttcgccg cgctcttctg ctcctacacc
     7981 gcaatatgca cctgctgtat tgatcaaggt aatcaccggt ctattgaact tttccgcttg
     8041 tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg
     8101 gatattttcc tgcagatccc gtcctttttg aataccgatc actgttaccg gtttgccatc
     8161 taatcgagcg attccaccaa caatcgcttc gtcatctgca aataaacgat caccatggca
     8221 ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc gttctttagc
     8281 tcttgctaac tgaacgacat catgtgctgt cttatccacg atctatccat cctttctgtg
     8341 tatgcaattc aagaagcgta tggattttct gtttcaattc catgcgggga acaatctgat
     8401 cgacaaaacc atgagataat aagaattcag ccttttggaa atcttctgga agttcctgct
     8461 tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat caagctttgg ggttctgcta
     8521 gaataatatc gccttccatt gcaaaactgg cagttactcc tccggtcgtc gggtccgtta
     8581 aaacagttag ataaaaaaga ccttcattgc tatgccgttt gacagcagca gagatttttg
     8641 ccatctgcat tagagaaaag attccttctt gcatccgtgc gccacctgaa gctgtgaaca
     8701 agacgactgg aagactttcc tttgttgctc gttcgaataa tctggtgatc ttttctccga
     8761 caatcgttcc catacttccc atgataaaat taggatccat cactccaata atgaagggaa
     8821 tcccctgaat ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca
     8881 tttttttaat tttttccgta tattcgggaa atgctagcgg atctttggtt acaagatccg
     8941 tatcccattc ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc catgcaccga
     9001 ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc accgatgtct ttcgtataaa
     9061 ggatatgttt gcaatttgga cactttgccc acatgttatc aggaacagat ggcgagttag
     9121 caggtttgct ctctctatta ggattgatcc gaatatattt ctttttctta aacagagcca
     9181 tctatgcgcc tcctatctcc gtttctggtg tccaattcgg taaaaattct tcttgtaaaa
     9241 atgctgtgct gtaatcacct gctatgactg caggatgact aatcaaatcc atctgaaatt
     9301 ctgcgttcgt gatgattcct tctgtgataa attcacttaa ggctcgctgc atcttcatta
     9361 atgcttcaaa acgcgtattt ccatggacga ttatttttgc aatcattgaa tcataatatg
     9421 gcgggatcgt atatcccggg taaactgcac tgtctactcg cagtcccatc ccacctgctg
     9481 gaagcagtaa gttttggatc ttccctggag aaggagcaaa atggaaagca ggattttctg
     9541 cattaatccg gcattcgatt gcgtgtcctt ggaatactac ttcttcttga cacacattca
     9601 aaggctcacc ggctgctatc tccacctgtt tttttactaa atcgattcct gtgaccattt
     9661 ctgttactgg atgttccact tggattcgag tattcatttc catgaaataa aaatcgcctt
     9721 cttcatccat cagaaactcg atcgttccgg cattttcata attgactgct ttagctgctc
     9781 gaacagcagc gtctcctagt gcttctcttt ttgtttgtga gatcactaca gaaggagatt
     9841 cttccagtac tttttgattg ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga
     9901 catgaccaaa tcgatcacct aagatctgaa cttctatatg gcgagcagga taaatgattt
     9961 tttccaaata catatcatca tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa
    10021 aatgctgtgg gagctcttct tttgattgaa ctttccgaat gcctttcccg ccaccacctg
    10081 cagctgcttt cagcatcact ggataaccaa ttctgtctgc tatttccaat gcttcttcta
    10141 ctgtagataa tacaccagta cttcctggaa tgactgggac atttgcttct tgcatcaatt
    10201 gacgcgcatt gatcttattt cccattgcat cgatcgttgc acttttagga ccaataaatg
    10261 tgatattgca ctcttcacac atggaggcaa atcggctatt ttcagataaa aaaccaaatc
    10321 ctggatgaat tgcttcggct tttgtcacga ttgcagcgct cagtacttcc tgaacattca
    10381 gatatgaatc aacggctttt gccgggccaa tgcagattgc ttcatctgct aactgtgtat
    10441 gaagtgcttc ggcatctgct tctgaatata tcgctacggt ttggatccct agttcgcggc
    10501 atgcacgaat gatacgtaca gcgatttcac cgcggttagc tatcaatact ttagaaaaca
    10561 tatttcacct atccaatcat aaaggttaat tccgcttcgg ctactttttt accgtctact
    10621 ttagcgattc ctttgcctat tccggctgaa gctttcactt tcaatatctc aacttcaagg
    10681 tataaggtat ctcccggtgt aactttctta cggaattttg ctttgtccaa tccgccgaaa
    10741 taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc
    10801 attgcttcaa caatcaatac ccctggcata acaggttctt ctggaaaatg tccttgaaaa
    10861 aagggctcat tgatcgttac atttttctta gcaacgatcc gttctccttc aaccatttct
    10921 tccactcgat caatcaaaag cattggataa cgatgtggaa tgatttcttt tatttcttgt
    10981 atgttcattg ttttccctct ttcctgcttt gcttattttt gctgaatcct aaagagtggc
    11041 tgtccatatt cgacaactga ctcgttttct acaaggattt ctgtgatcac tccgtcggcc
    11101 tcactagtta tctcattcat cagtttcatc gcttcaataa tgcagataac ttctcctttg
    11161 gcaacttgat cacctacttt tttgaaagct ggtttgtcgg gcgctggttg cagataaacg
    11221 attcccacga ttggcgaaac aacctcgatt ccatcaaata ctgccgattc tttttctttc
    11281 attatttctg gagcagttgt tgcttgttca gatatgacat tgactggctc cgaaactggc
    11341 aacgtttcgt ttgtttgaac catattcatg cctcgacttg ttttattttt attcatatac
    11401 aattcaaatt gaccttcacg caaatcgaat tccgttagcg aggattgatc aaattgactg
    11461 actaattctc tgatttcatt gatatccaaa attattcctc ccagcgtttc atgcacagaa
    11521 ccgcattatg tccgccaaac cctaatgaat tactcattgc ataagtaaaa cttgcaggtt
    11581 tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc ttgtttttct acattgatcg
    11641 taggtgggat aaattgatgt tctaatgcca gcaaagttgc caaggcttcg atcccgccgg
    11701 ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat
    11761 tttcacctaa tgcgatttgg attgcttttg cttctgcctg gtcattggct ggtgtacttg
    11821 ttccgtgagc attgatatat ccgacttgtt ccggctgtac accagcttct tccatcgcaa
    11881 gcttcattgc tttagatgca ccagaaccat ctggagtagg agctgtcata tggtaagcgt
    11941 cacagtttgc accgtatcca acgatttctc catagatttt ggcaccgcga gcttgtgcat
    12001 gttctaaaga ttcaagaact agaacgcctg ctccttcacc catcacaaaa ccattccgat
    12061 tttcgtcaaa cggaatcgaa ccacgattag gatcagttga ttctgagagt gctgttaaag
    12121 ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc tgttcctcca gcaagaatga
    12181 catcagaata gccatgtttg atgttacgga atgcttcccc aattgaattc gttccgctag
    12241 cacaagctgt tacgatagct gtacaaatac cttttgcacc tacacgcaaa gcaatatttc
    12301 cagctgccat gttggcaatc gccattggta cgaacattgg agaaactcgt tttgcacctt
    12361 tttcatgtag acgaataact tggttttcga ttgttggaag accaccgatc ccagatccga
    12421 tgatgacgcc aaaccggtct tgattcattt tttctttatc taaaccgctc atctccattg
    12481 cttctaaagc agcgtagatt ccatagatcg aaaacatatc cattcgtttg ctatcttttt
    12541 taataaaata tttatcgtaa gggaaatctt tgacttcacc cgctaaggta atccctgttt
    12601 ctgaagcatc aaatttagtg atcggtgcga tgccattcgt cccctctttt aagctattca
    12661 aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc atagccagta attaccactc
    12721 gattcatttg tttgctctcc ttttcttatc cgtgcatgac caatccgcca tcgacattga
    12781 tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt
    12841 ctacttgtcc gaaacgctgc atcggaattt gcttttccgc ttgttccttg actttgtctg
    12901 ctaaaacttc cgtcatatca gttgtgatga atcctggcgc aatggcatta catgtaatgc
    12961 ctctagttgc agcttctcgt gccacagatt ttgttaatcc tactacgcca gctttactag
    13021 ctgcataatt tgcttgacca atatttccaa tcaacccaga tacactagag agattaatga
    13081 tggccccttc tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc
    13141 caatcaaatt gatatccaga cattttttga aatcttctgc atccattcgc atcacaagtt
    13201 tgtcatttgt gattccggca ttgtttacta acacatggat cgatccaagc ttttcttctg
    13261 cttctttgat catttgtccc gctttctcat aatcagagat gtcaccggaa actcctacgc
    13321 attttacacc gaatgcttcg atttcttcta tcttttcttt tggaatctct ccgcgaccat
    13381 tcaaaatgat atttgctcca gcttttgcaa aagctaaggc catcgctttg ccgattcctc
    13441 ttgtactacc tgtgataaat acattttttc cttttacatc cataacgttc ctccttatag
    13501 tattgctatt gtttcatcaa atgtctgctt attctcaaca cgcagaactg gtattgtttt
    13561 atcgattttt ttcataaatc cgcttaatac ttttccgggg ccgacttcga tgacttgttc
    13621 gatgccgatt gtttttaact tatggatact ctcatagaaa cgaacaggtt gcatgacttg
    13681 ctgttcaagt agtcccgcga ttgtttcttt cttcattatt tccgttgttg tgttgcttat
    13741 gataggaaaa gaaggttcag agaattgaat ctgacttagg tccttcgcca gtttttttgc
    13801 tgccggttct aaaatagctg tatgaaaagg cccgctgaca tttagaggga tcattctttt
    13861 gaaccctttt tcttttaata gtaaaacagc ttcgtctact gctttttctt cgccaccaat
    13921 cacgatctgc tgaggtgtat tataatttgc aggagatacg atgccgtact tgctagcttc
    13981 atgacagctt tcctcgatcg tttcaatcgg cgcattcatc actgcaacca ttttcccgct
    14041 tccagctggg gtagcttctg tcatataagc gcctcttttt gctactaacg caactgcttc
    14101 agtgaaggat agcgcattac tagctactaa tgccgaatat tctcctaaac tcagtcctaa
    14161 agctgcatct gggatgattc catgctcctt caataagcga taatacgcga tgctgactgt
    14221 tagaatagct ggctgtgtat attgcgtctg atccaaacgt tcattttctg taaagcaaag
    14281 ttcagccata tcgtagccga gaatctcact tgcttcatca aatgtctgct ttacaacggc
    14341 ttcttcatat aaatcttttc ccataccttg atattgtgca ccttggccac taaataagaa
    14401 tgctgttttc atgtttttct ccaatatatc cagaatatat gtttgattct tcctattcaa
    14461 aaaggccgag acattcctat ttcaggaaat gtcccagccc ccaacctaat tttaatgatt
    14521 atgcagtttg tttttctacg taagtcacta aatcttgaac cgttttgatt ccgtcttctg
    14581 tttcgatttt cacgtcaaat tcatcttcaa tttcattgat gatttggaat aaatccaagc
    14641 tgtcagcttc taagtcttct tggatgtcag tagtcaattt gatttcttca gtttcttttc
    14701 ctaattcttc tgcgatgatt tcttgaattt tttcgaatac cataataata tattcctccg
    14761 atattgtttg ctttttattt ttataattta acagtctaca actccatgag aatacttccc
    14821 catgttaaac ctccgccata gcctgtaaat attactttct gctgttttcc taaggtgatc
    14881 tttccactag aaactgcttc atccaataag atagggatgc ttgcagcaga tgtattccca
    14941 tatttatcca tattggtcag gaacttttct tgcggcatct ttacttttcg ggcaattttc
    15001 tcaataattc tgaggtttgc ctgatgcaaa agcagataat caactggttt gtctttcatg
    15061 acttcttgga tattgtttgc aacgtctcgt acggcaaaat caaaaatatc tctgccagtc
    15121 atttgcaaat aatacgaacc ttctgaatct tctgaataga acggattttt attttgctgg
    15181 tagccagcgg tcaatgagct acttctggta ccgtcagctg ctaatttttc tttcagcaat
    15241 tttttctgtg gattcgcttc taagagaatc ccacctgctc catcgccaaa caacacagca
    15301 gtcgtacgat cagaccaatc gattagctta gatagtgttt caccaccaat caccagtccc
    15361 ttagttgagc cgcagcggat cattttttca gccatgctta acgcataaac aaatcctgag
    15421 cacgcagcac tgatatcaaa agcgatagca tgagttgctc ccaatttccc ttgtacttgt
    15481 gctgctacag atggtgtcgt gtaatcgggc gacattgttg ctactaaaat aaaatccaaa
    15541 tctttagggt ttagattttt ctgttttatc aatttttccg ccactaaaat gcataaatca
    15601 gaagtctcct gatttgttac acatcttcgt tcacgaatac ccgtccttga cgaaatccat
    15661 tcatcagagg tatccatcat cttggaaaga tcattattag taataatctt ttcaggaagg
    15721 acgctggctg ttgcagtaat cattccaaat tgttccatta aatatcctca cttatagtct
    15781 tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg catgctgttc ctccgtagcc
    15841 attccgtcta agatgttctt gaccatttca cgatgaaaat gctgatggac ccgaaatagt
    15901 aactttcctt ttttcgttag tcccaacttt acaacgcggc ggtcatcttc actgcggata
    15961 cgttcgacat agcctttttt tactaacttg ttaatagcag tggtcaaggt cccaacagta
    16021 attgatagtt ctttggctac ttctgaagtt gtttttttct tgtacatccc gattgcctcg
    16081 atcgtgtgca tctctgtgat tgacagatct ttgaattgag attttttcag ctcagactct
    16141 tcgattgtca aaatatcatt gaagacacta acaagataat catttactgt ttttaaatct
    16201 ggttccatgt tccaccgtcc actaattagt ttgattatca aatcttttga tgttcaaact
    16261 atatcttaac ttttttc
//
LOCUS       DAZKCY010000058        15792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000058 DAZKCY010000000
VERSION     DAZKCY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.058"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..327)
                     /locus_tag="ACG8XC_001683"
     CDS             complement(25..327)
                     /locus_tag="ACG8XC_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9191379.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(547..913)
                     /gene="ssrA"
                     /locus_tag="ACG8XC_001684"
     tmRNA           complement(547..913)
                     /gene="ssrA"
                     /locus_tag="ACG8XC_001684"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1084..1857)
                     /locus_tag="ACG8XC_001685"
     CDS             complement(1084..1857)
                     /locus_tag="ACG8XC_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9191380.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2001..2351
                     /locus_tag="ACG8XC_001686"
     CDS             2001..2351
                     /locus_tag="ACG8XC_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFP9191381.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2332..3048
                     /locus_tag="ACG8XC_001687"
     CDS             2332..3048
                     /locus_tag="ACG8XC_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFP9191382.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3048..4496
                     /locus_tag="ACG8XC_001688"
     CDS             3048..4496
                     /locus_tag="ACG8XC_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9191383.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4566..5075)
                     /locus_tag="ACG8XC_001689"
     CDS             complement(4566..5075)
                     /locus_tag="ACG8XC_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191384.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5065..5790)
                     /locus_tag="ACG8XC_001690"
     CDS             complement(5065..5790)
                     /locus_tag="ACG8XC_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191385.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6013..8133)
                     /locus_tag="ACG8XC_001691"
     CDS             complement(6013..8133)
                     /locus_tag="ACG8XC_001691"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9191386.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8363..9541
                     /locus_tag="ACG8XC_001692"
     CDS             8363..9541
                     /locus_tag="ACG8XC_001692"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFP9191387.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9557..10315
                     /gene="aroD"
                     /locus_tag="ACG8XC_001693"
     CDS             9557..10315
                     /gene="aroD"
                     /locus_tag="ACG8XC_001693"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP9191388.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10338..10823
                     /gene="ybaK"
                     /locus_tag="ACG8XC_001694"
     CDS             10338..10823
                     /gene="ybaK"
                     /locus_tag="ACG8XC_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFP9191389.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10894..12147)
                     /locus_tag="ACG8XC_001695"
     CDS             complement(10894..12147)
                     /locus_tag="ACG8XC_001695"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9191390.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12264..13613
                     /gene="gdhA"
                     /locus_tag="ACG8XC_001696"
     CDS             12264..13613
                     /gene="gdhA"
                     /locus_tag="ACG8XC_001696"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP9191391.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13725..15071
                     /locus_tag="ACG8XC_001697"
     CDS             13725..15071
                     /locus_tag="ACG8XC_001697"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP9191392.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15379..15765)
                     /locus_tag="ACG8XC_001698"
     CDS             complement(15379..15765)
                     /locus_tag="ACG8XC_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9191393.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5164 a         2825 c         3173 g         4630 t
ORIGIN      
        1 gctacctttt ttaggtagcc tactttaatc tttatacctt tctggatcaa cgaaagtata
       61 cttcaaatag tcataacgcc gatgatcgct ccgtgcgtct ggcacatcag tcacgatatc
      121 aaacaaaaaa tacacatctt tcttcattct ggtttttgtt gccggaattt taaagtagtt
      181 cttattagag taatagagat tgattaataa gctatcttcg attgctaaaa agaaaacttc
      241 tgaatcccac accttataaa aatctttgac aaatctattc gaaggatcaa atttaaacca
      301 taattgtgtt tttccttcca tcaacataat gttcacctca aaaagagtat acgaactaat
      361 gttctttttg taaagtagga atatcattga taatatggat ggaacatata tattaaaaag
      421 tgttcttttt gaatgctatt ttgaatgcca ttttcattca tttaacgaaa atctgcgaaa
      481 ataaaaaatg ctcaaaccct tataaaataa ggatttaagc atctagcgaa atctgctgaa
      541 atctcacaat ggagacggcg ggagtcgaac ccgcgtccaa acacatcgcc acttaaaaat
      601 ctacgttcat agtcaactca tttaaggatt cactttacag cttgccgagt gacaggcatt
      661 ctgtatcgct agtctgatgt tctctttttc aacttacaga cggaaagttg aaacgtatcc
      721 cactaaagtt aggaccctta ctcgagcaca tgggcgatgc cgagaggatc tacgctaact
      781 gtttttaggc agctaaagcg taagagttgt tttcgttttt agcagttata tttaactgta
      841 acgtttttac gtagacgtaa cctacgaaac gcaattcaag ctcgactgtg cctgtcgaat
      901 ccgtaacgtc cccgttagct aacaagggtt acgtgtattt ttcatacaaa attagtatag
      961 catatattgg gataaattca ataaaaaagc gaatcatatc ccattattta tataataact
     1021 gcccctttct taagaaaaaa taagcgattt tcttaagaaa agaggtagct tcaaaaaaag
     1081 atcttaaatc agatcaaaat acttcaatgc gtggacaatt ccgccttctg tatttttctt
     1141 tgtgataaag gtagaaagtt cttttaatcc gtcataggcg ttgcccatag cgattttgtt
     1201 gtcgcaagct tccaacaagg caaaatcatt cggaccatcg ccaaatccaa aagtaggaac
     1261 tttttccagc tgaagttctt tttgcagacg tttgacacct gccccttttg agattccttt
     1321 cttcactgta tcgatcgagt aaggaccatt ccgataaaaa gttagctcag gaaaatgttc
     1381 gtgatagtat tcgtcacctg attgagataa gatcagcaac atatttactg tgttttcttt
     1441 aaagcctaaa ggatcaattt ccggaacatc tgaatgaatg aagtcgtaag cattgatcat
     1501 tgtttggcta tgaccggtac accagatttt ctgatcgtta tagtaagaaa gttcatgacc
     1561 gttcaactgt acatgatcat acattttttg acattcttct gaagaaaatg tttgagaata
     1621 gatttcttcg ccgtctactc gaatgaaaga accattcatg gtgaccgcag aagtaatgcc
     1681 cgcatcttcc atgatagagt ggatttctgc ttctgtacgt cctgtagcga tgacggggag
     1741 aacattgttt tcttttaatg cgttcatagc ttttgccact tcgggtgtga tttttgattt
     1801 cgcatctagt aatgttccat caagatcaaa gaaagtgatt gcacgataat ttgacacaaa
     1861 taaaactcct tttttattgg aataagttta tcttaacaaa gaatgaacaa aagacaaacc
     1921 tttcgtgaag aattcctgaa catattggtt cagtcaacaa tttagagtat actataagaa
     1981 gagaatttag gagggaaaga atgatttttt tcagtattgg tatcttattc ttaataattg
     2041 gcagtctttt tcggattctg ccctcaaaag gaaatctgcc tttttatggg tatcattcgc
     2101 ctcttgccgc aaaaacagac gctcattggc ggctggctca aaagacaagc ggaaactggt
     2161 tttttcttat gggcttactg atggcattga tcggctatta tttaaagaca tctgggcata
     2221 cgaattattt tctgattgaa atgcttctgc tagtatttcc aattatgcct atttttatta
     2281 tgacagaaaa gaaattacag aaatatgacc tggaaactgg agggaacgat aatgaatatt
     2341 ttaatgattg aagataatca atctgtttct gaaatgatgc agatgttttt cttaaatgaa
     2401 ggttgggaag caactttcaa gtatgatgga aaagaagggc tcgatgcctt tttagaaaac
     2461 ccacaacatt gggatatgat tacactagac ctgaatttgc ccacactgga cggtatgcta
     2521 gtaggaagag agatccgcaa agtttcaagt actgtgccga ttatcatgct gacagctaga
     2581 gattctgaaa gcgatcaggt gatcggctta gagatggggg cagatgatta tgtcacaaaa
     2641 cctttcagtc ctctgacgct tattgctcga atgaaagctt tgcatcgccg tagtgagttg
     2701 gcagaagcaa gagaagaagc agtagtttca gatgatcatt ttgatatcga gactagtcat
     2761 ttcaagatga atacgaaaac acgagaagcg tatctggaca accggccaat tgaaggactg
     2821 acgcctaaag agttcgatct attgtttact ttagcaaaaa aaccacgtca agtattctct
     2881 cgggagcagt tgcttgaatt agtctgggat tatcagtact ttggtgatga acggacagtg
     2941 gacgcccata tcaagaaatt acgacaaaaa atcgaaaagg tcggaccaca agtcatccaa
     3001 actgtttggg gagtcggcta caaattcgat gattctggtg ttgcctaatg cgttatttgt
     3061 atcagcaact cttagctttc tgcggaatgg tcgcgttgat cttattgatc gttggagttt
     3121 cttttacaca actgacaaaa caaacgattg aagaaaacaa ttaccagcaa ctgtttggat
     3181 atgctgaatc agttgaaaag acaacgcaaa cctatgccga tacgctcccg cagtttagtc
     3241 aagatcaggc gtttcaaaat gcattgctgc tgacagagca agtactgaca gaacaaaatg
     3301 tgaactttat atttatcgat aaaaacgaac gtgtcattta tccgactaat acagctttac
     3361 tgaattttac cttaacagct gatcaatgga aaaatctgag gaatggacgt caagaaaaat
     3421 tcacatcgaa taaaaatatt ttaggacaag cacaggcgac ctcttacgca cttgtgccat
     3481 ttaatttgaa tcatgaattc tatggagcac ttgtagttag tcaacctgcg cgaaatatcg
     3541 ataacagcgt tcgtccaatt acactgaacc tgttcaaagg atttattttt tccagtatcg
     3601 ttgcagttat tgccagctat gcttttgcag cattccaagt taagcgtatc aatcggttaa
     3661 ggaatgcgac gaaagaagtg acgaatggta attttgatgt acagctcccc gtacatgaca
     3721 aagatgaatt tgatgagtta gcagatgatt tcaataaaat gacgaattca ttgaaagaat
     3781 ctcaagcaga aattgaagaa caagaaaatc gtcgaagaca atttatggca gatgcttctc
     3841 acgagatgag aacacctctt acgacaatca acggacttct ggaagggtta gaatacaatg
     3901 caatccctga aaatcagcga gaaaacgcga ttaaattgat gaaaaatgaa acagagcgtc
     3961 tgatccgttt ggtgaatgaa aacttggatt atgaaaaaat ccgtacgaat caaatcagta
     4021 tggtcattaa aaaattcaat ggaacagaaa cattgaaaaa tattgttgca cagttagaag
     4081 ctaaagcaga agcagcagga gatacattga cattgaaagc cgttgatgat attgatgttt
     4141 acgctgatta tgaccgattt gtccaaatca tggtcaatat tattcaaaac gcgatacagt
     4201 ttactgaaaa cggccaaatc acgattactt tagaaaaagg ctatcttgaa acgatcatca
     4261 cgattgaaga tacgggaatt ggaatgtctg aacaacaaat gaaaagtatc tgggatcgtt
     4321 attataaagt agatccttca agaaaaaata caaaatatgg tgaatcagga ttaggtttac
     4381 caatcgtgca gcaacttgta cgcttgcata aaggaaaatt agaagtagaa agcgaactag
     4441 gaaaaggaac aaaattcgct gttcgtttgc ctgacgtaga aatcaaaaaa gagtaaaaaa
     4501 caagcggtat aaaagatcat gaatcgatct tctataccgc ttgtttttac tataaaatga
     4561 ttgatttagc ccgcgggctc taaacgatca atataaggac tttgagcaat agtagcatca
     4621 tgagtaaaga gtaagatacc ttgattttct tcatgagcta aataagaaag caagtggagt
     4681 agctcttgga tctccaatac actaagttca tcaaatgtat cttcaataac aatattgttt
     4741 cgcttggaaa gcaaggcatg aagtaattgc agctttactt tttcaaaaga cgtcaactca
     4801 gctgccgcac aattaagcaa ggaaggagag atatcaagtt tttcttgata ggccaaacga
     4861 tcttgtttga tttctttttc cggtacatcc aacaggacat ggtctttaag attcagataa
     4921 ggaaacatcg gccaattttt ttggatgatc gcaaaattat ccagttttgc aacaagaacc
     4981 tcttttttca agtgctccaa tattttatgc tgtttttcga gaggacacaa aataacggtc
     5041 aagtgttcag catgaatcaa atattcaggt ttcattacgt ccaggcctcc tactttgcaa
     5101 atgatggata aaaaaagtga aagggaatag actaagaatg agacagcatc cactgagccc
     5161 aataagagtt tgggcaaatc ccttaagtat atctgtgaaa aaaagatcat tttggaaatc
     5221 gatggtaaaa aaagtctgtt gatccatcgg aattgtaagt ccgcttaccg aagtcaagat
     5281 atcacggata tctggcagtt cagtagtctt taagacaaga aaattgatcc tttgaagaag
     5341 cgcttcataa actgactgga agcttacgat ccacaataaa accaagatac aacaaagaaa
     5401 gagaggaaag aacagctgcc agaaaataaa aaggaataat ttggttttag aaagaccagt
     5461 caggtgccaa ttgatcaatt cctgcttgtt ttgcctgact gaatagtaaa agaaaaagat
     5521 aaaaaggaag gcaaatagaa aaaataaact gaatatgcct tttatataaa atcggctagc
     5581 taattctaaa gccggaagaa gctctgtaga tgcagaagcg ccaataaatg aaagccgatc
     5641 ttggacttga ttggctaaag ccgattgcaa atggtaaaga gtcaggcatg ctatcaataa
     5701 aaaagcagaa aagcttgaaa ataaacaata gagtattgtc tgctttttgt aataaattaa
     5761 tcgagcagta gcatagcgga ttattttcat acttttcacc tctataaaaa gtatagaaaa
     5821 tgaatatgaa taaagtatga aattccttta gtgaaatcac gaattaaaac cagaaaatat
     5881 tttgtaaaaa gcaagaggtt gtgaagaagc tgccctattc tattaccgta gatgcaagct
     5941 tttgaacact gtttattcga aaagaggagc agcgacaata attttgtcgc tgctcctttt
     6001 aaatttattt tatcattcgg aagaacttgt ggtcgttccg tgaagttctt ggtacgtttg
     6061 cgttttataa agatcaacag ttgattgtcc gttgtgttca ctaaagacac tagttgactt
     6121 gtcaccaaga gatttttcag cttcttttaa tttctctaag ccattcttgt agtcataatc
     6181 actggtatca attgggtcta atccactatt cgtatagaac cgaagaagat caccattatt
     6241 gatttggtca gaaacagcta actgtttatc aactttttct ttcagtgaat caatttcatt
     6301 ctgtacttcc tcagtaggtt cagtaatagc ttcaccagtt tcgttactat agatcgtacc
     6361 accgtagtaa gtatattttg gtgtaacaaa atcaccatca cggaaagaaa caagctgttc
     6421 atgatctttc gagaatagat cttgtcctaa ctgaatataa ttttttgtat ctacacctag
     6481 taagtgaagt aatgtaggca aggcatcaac ttgtccgccg taagtgtgat tgattccgcc
     6541 ctgtgtttgt ccagggatat ggatcatata agggacacgc tgcatttgtg cattgtcgta
     6601 gtttgtccaa gtagaacttg tttttccaac taattcagct agatttttat ttcttgaatt
     6661 cgagacaccg taatggtcac cgtaaaggac gatgactgaa ttatcataca agccgctttc
     6721 tttcaaataa ttgaagaatt cttgtacggc ggtatctaaa taattggctg tcgcaaagta
     6781 accgttgatc gtttcatcat ttgtattggc taacgggaat cctgcttcgt cattggtgaa
     6841 ttttgaataa ggataatggt tagatacagc aatgaatttt gaataaaatg gttgttgcag
     6901 acgttccaag tattgtacgg attgttggaa aaatggttta tcatgcaacc cgtattggaa
     6961 cgaattgtca tcattaacat cataataaga agaatcaaag aaataatcat atcctaaatt
     7021 cttgtatgtc tcgtttcgat tccagaatgt accagcgttc ccatggaata ccgcactaat
     7081 gtaaccttgt gtttgactta aaatatcagg tgcagcttgg aaagtatttt ttccgcctaa
     7141 ttgtgtgaac aaggagcctt gattcaagcc aaacaatgaa ttttccatca gcgtttccgc
     7201 atcgcttgtt tttcctgctt ttacttgatg gaaaaagttg tcaaaactga acgtgctgtt
     7261 gctgtggtaa agactattga tgaaaggtgt aacttcatgc tggagaccat tctcatctgt
     7321 caatttataa tcgatcagga attgctggaa gctttctaaa tggatataaa tcacattttt
     7381 tcctctagcg agcccgaaca aatcgtcatt tggtgctgca taatgttctt ttacataatt
     7441 ttctacttct ttcatatcat tgggacttgc ttctgcccgc acttggtttg tctggtaagt
     7501 ttgaacaccg tcataaacca tgaaagcatt caagccaaga tatttgacca aataatcacg
     7561 agagaatgtt cgagtcagca attctggacg atcagcttcc gccataaaaa gatttcctga
     7621 gaaaatcata actgctaatg tagagatcgc aaaagccatt cttgctcgga tcgggcgagg
     7681 gtccaacttg attttttttg caatcagcag aacagtcaag ataataaaat cgatccaata
     7741 gatcacatca tatgggcgaa acagtcgaat ggcactttct cccagaccac tcgctacttt
     7801 cccagcacct aaaatcgtgt tgatcgtaat gaagtccgtg aattctcgat aataagtgac
     7861 attcgaaaaa acaagcaatg tcaaaagaaa ataaatgacc atcatcgtga tataagcaac
     7921 tctagggcgt ctgatataaa gagcaattcc aagtaataac aatgtagttg caatagggtt
     7981 gataaacaag ataaagtatt gaatgccact ttcgattccg agatggaaat caactaaata
     8041 ggcaaaaatc gttttcaacc aaagtaaaac gactagtaaa gaaaaaaatc cgagcctctt
     8101 attcaaaaag gcaggtgttt ttatacattt caagataatc ttcctttctg cacttcttaa
     8161 tcacagaagt gtcctgaaac catgttacac agctcactta ttctcattta ttttactagt
     8221 atgacaatat agtgtcaatt gaaaccagaa gtttatgctg tttcattaag gttttcttta
     8281 agcttcatga aaaaatgagc agtaagacaa gtcctatgtt ttttgttata cttgggaaga
     8341 gaaagctaaa gcaggtgaag gaatgaaagt aaaagtaaaa aaaagagcaa gcaatcgatt
     8401 gaaaaaagga tatccactga tccaaaaaga agaccttgct catccccaaa aagaaaccac
     8461 gcaatgggtg gaattagttg attcgcaaaa ccaatttatc gcaaaaggat atctaggaaa
     8521 gcagaataag ggaatcggtt gggtattgag ccagcagaac atcccatttg atcaatcttt
     8581 ttttgaagaa ttttttgtga agtcaaaaga aaaaagaact ttcttttttt cagatgattc
     8641 tactaccgct tttcgtatat tcaatggaga aggagacggg atcggcggtc tgattgttga
     8701 ttattatgca ggttatgctg ttttttcttg gtataacgaa acgttatatg cacatcgtga
     8761 attattgatt caagcttttc gtgaaatcta cccggaaatc ataggaggct atgagaaaat
     8821 tcgatttgaa tcagtaaatc tgcctgagag ccagttcatt tacggaaaag aggcaccgga
     8881 acccttgatc gtaaaagaaa acggagtaaa atttgccgtt tatttaaatg aaggattaat
     8941 gactgggatc tttttagacc aaaaagaagt acggggatca ttagcagaag gtttcgcttc
     9001 tgggaaaaca gttttgaaca tgtttagtta taccggcgcc ttttctgttg ctgccgcaat
     9061 gggaggggca atcgaaacaa caagtgtcga tctagcaaaa cgcagtttaa aaaaaacaca
     9121 ggaacagttt gaagtgaatg gtttggatgt cgatcagcaa aaaatcattg ttatggacgt
     9181 attcgagtat ttcaaatacg ccaaaagaaa acaattttta tatgacatga ttgtattgga
     9241 tccaccaagt tttgccagaa ataagaaaaa agtctttcgc gtagcaaaag attatgggga
     9301 actggtcaag gatagtctgg atattttagc agaagacggg atacttattg cgtctacaaa
     9361 tgctgcgaat gtctcgatag aacaattcca aacaatgatt gaagaggaat tccatgccaa
     9421 aaatgtttct tttgaacaac ttgctttata tcgtctgcca aaagatttcc agacgacgga
     9481 taccttttta gaaggcaatt atctgaaagt gtttgtttat caaattaaaa ataaccatta
     9541 gaaggagcga tgcagaatgg ttcgggtaaa gggattgaat ataggaagcg gtagaccaaa
     9601 gatttgtgcg cctcttatcg gggccgtccg agaggaagta ttacgtgaag cagttcttgc
     9661 acgtgaagca ggggccgatt tagtcgaatg gcgagtcgat cattatcgtg aagttttttc
     9721 ttttgatgaa gtagcagaaa cattggttct tatccatgag atgcttgatg gactgccgtt
     9781 attatttacg tttcgtacct tagctgaagg cggagaacaa gacatatcga tccgtcagta
     9841 tcgcgacctt tatgaacatg tgatacacac aggattagta gatatggtcg atatcgagtt
     9901 gttcaaagta gaaagtctag gaaaaagact gatagaggaa atcaagtctt taaacattcc
     9961 tttaattatc agtagtcatg attttaaaga aacaccagca gaccctgtct tgctttaccg
    10021 attgaatgtt atggaacatt ttggagccga tatcggaaaa ctggcagtaa ctcccaataa
    10081 tgaacgagat gttttgcggt tgatggagtt gactagacgg gcaaatgctt ttgtctccat
    10141 gcctattatt acgatgtcca tgggaaatct tggtaaaatt tctcgtctgg ttggcggaac
    10201 gacgggttcc gttatgacat ttggttcctt aagcagcgat tctacttctg ctccaggaca
    10261 attaccggtc aaagcattga agcaaattat tcaattatta aatgaaccgt tgtagaacaa
    10321 gtttgaggtg atattttttg gcaaagaaaa aaatagtaaa aacgaatgca atccgtatgg
    10381 tcgaacaaaa aaagattccc tatacagagc acgaatatga gtgggacgaa agccacctaa
    10441 gtgcgtcatc ggtcgctgaa cagcttccag aaagccaatc tcgtatattt aaaacactgg
    10501 ttgctgtggg caatgtgacg gggcctcttg ttgctgtgat accgggagaa gccgaattga
    10561 atctaaaaaa attagcaaaa gtttcaggga ataaaaaagt agaaatgctc catttaaaag
    10621 atttggaagc aacgaccgga tacatacgtg gtggctgctc gcctataggt atgaaaaaac
    10681 tatttcctac ttatttagat caaattgcag aaagctatga acaaatcatt gtctcggctg
    10741 gcagaagagg gctacagatg gagttagcac ctcaagatat ctgtgcatta acgagcggac
    10801 aatttgcaga tataaaacaa taaatcatac gtataaaaga aagaaggagg ccgggatgtt
    10861 gtttgtccag tctccttctt tcttttttat acattatgct ctttgcccgg cattcattcc
    10921 tgctacatgc cctgtagcga aagccgctgt gatattaaag ccgccagtat aaccattgac
    10981 atccagtaat tctcccgcaa aatacagacc atcaatgatt ttactctcca atgttttagg
    11041 ataaatttcc tttagagaaa cccctccgcc agttacaaat gatttctcaa taggaaaagt
    11101 tttgttgatt gatagcttaa actctttgca tagttgaaca aaatcttgaa tttgtttttg
    11161 agaggcttgt tgtcctgtca gatgatccat ttcaagccgc tcaagaaaga aaactaacaa
    11221 tcgctcaggc aatataccat gccaagcatt gaccaagttc tttttggttg ctttactttt
    11281 ttctgtcaat atatgaataa gctcttgttt ggtttgtgta gggaaacaat ccagagaaag
    11341 tgtcactggt tctcccgttt tctctaattc tttattgaca aaacttgaac aacgcagtgc
    11401 agccggacca gaaataccga aatgggtgaa aagtaaatcc atcgtatgtt cagtcaatac
    11461 acgacctttt ttatttaaaa cgcgtaaagt gatgtcttgt aaagaaagcc cttgaagtgt
    11521 tttctcctga atatacggtt catccgatat caagggggac tctgtcgcat aaagaggcgt
    11581 caccgtgtga cccactcgct tcacgatctt gtacccatct ccagtagaac cagtagatgg
    11641 ataagtccgt ccaccagttg ttaagataat ggcaggagca tggaactctt cgaattccgt
    11701 gcgtataccg taaattttat gttctttgtg gatcaatttc tctactcgag tagagaatag
    11761 aagcgtcaca tctagttctt tcagacgatt gagcaatgct tcgataatcg tttttgattt
    11821 atttgttaca ggaaacatcc gtccatgatc ctcttctttt aaatgtacgc cttgtgattc
    11881 aaagaagttc atgatatcga aattattcca ttgcgcaaac gtactatata aaaactttcc
    11941 atttccagga atatgtgcaa tcaaatcatc aacaggacga ttgttcgtaa cattgcaccg
    12001 accaccacct gtcatcagta gtttttttcc tgcttttttg tttttttcga tcaagagtac
    12061 gcgtgcgcct tgttcagctg cactgatcgc tgccatcatc ccgcttgttc cagcgccgac
    12121 tacgatcaca tcatacagtt tttccattcc ctcacctcaa ggaaagtgta gcatatctga
    12181 agaaagaaaa cagaaaaaaa tgttttgagc caaaagagaa aatgagctaa aatgagggaa
    12241 gataagcaaa ggagcgtgga acgatgacaa acgcaacaga atatgttcaa gcaattcaag
    12301 aaaagctaca tcaaaaggat caagggcaag aagaattttt acaagcaatc gatgaattca
    12361 tgcccacggt tatgtctttt ttggacactc atcccgaata tatcgaaaag aatatcttag
    12421 aacttttaac ggaaccagaa agagttattc aatttcgagt gccttggcaa gatgatgaag
    12481 gaaactggcg agtaaataga gggtaccgta tccaatacaa ctcagctatc ggcccttata
    12541 aaggtggatt gcgcttccat cctagtgtca atctaagtat cttgaaattt ttggcttttg
    12601 aacagatttt caagaatagt ttaaccggtc tgccaatcgg tggaggaaaa ggcggaagtg
    12661 acttcgatcc taaaggaaaa tctgataatg agatcatgcg tttttgtcaa agtttcatgc
    12721 tggaattgtc aaaacatatc ggcccatcga ttgatgttcc tgcgggagat atcggtgtag
    12781 gagctagaga aatcggctat ttgttcggcg aatataaacg attgaaaaaa tatgatactg
    12841 gtgtgttgac aggtaaacct ttggattttt ggggaagtaa gatacgtacg gaagctactg
    12901 gttatggatt agtgtattat gtcaaacatc tattgaacga agaaaaagat tcatttaatc
    12961 aaaaaacagt ttttgtttcc ggaagcggga atgtagccat ttatgcaatc gaaaaagtgc
    13021 aagagctagg cgggaaagtt gttacatgtt cagattctag cgggtacatc tatgatccag
    13081 aaggaataga tgtgaaacta ttgaaagaag tcaaagaaat caatagacaa cgtttgacgg
    13141 aatacgcaaa ccaacgtcct tctgctattt atcatgctgg agaatctgtt tggacattga
    13201 aagaaaaagc ggatatcgct ttgccatgtg ctactcaaaa tgaaatagac gaaagcttgg
    13261 ccgaaatctt agtagaaaat gggatcaaaa tcgtagcaga aggcgctaat atgccatgta
    13321 cagtaaaagc tgtttctgtt cttcatgaag caggcgtgtg gtattgtcca ggaaaagctg
    13381 ctaatgctgg aggagtagca gtatctgctt tagaaatgag tcagaacgtc cagcgattga
    13441 cttgggaaag agagcaagtg gatgagcagc tggatcaaat catggaaacg atttaccata
    13501 cgtgtcgcca aacagcagcc gagtacggcg acgaaaaaaa tctgcttctt ggagcaaatg
    13561 ttgctggatt tgaaaaagtt gccaaagcaa tggcagcaga aggtttagtt tgaaaaatga
    13621 aactttcttg gaaatattgt cagcattttg tttatttgct tgaaaaataa taaaaaaaaa
    13681 cgtaaagtat tacctgtaga gtaaacctat aaggaggaga acaaatggca cacattcatt
    13741 ttgactattc aaaagtagca ccgtttgtca acgaacaaga gttagggtac atgcaaagcc
    13801 aagtaacagc agcccacaac gaattacggg aaggtactgg cgcaggaagc gacttccgcg
    13861 gctggatcga tttaccaacg aactatgaca aagaagaatt tgcacgtatc aaagaagcag
    13921 ctaaaaaaat ccaatctgat tctgaagtat tagttgttat cggtatcggt ggttcttatt
    13981 taggcgctcg tgcagcgatc gacttcttga atcacacatt cttcaattta ttagatagca
    14041 acacaagaaa agcaccacaa atcttctttg ccggaaattc aatcagctca acatatattg
    14101 ctgacttgat cgaagtaatc ggtgatcgcg acttttcagt aaacgttatt tcaaaatctg
    14161 gaacaactac tgaaccagcg attgcgttcc gtgtattcaa agagcttctt gttaagaaat
    14221 atggacaaga agaagcaaac aaacgtatct acgctacaac agataaagca aaaggtgcag
    14281 ttaaagtaga agcagatgca gaaggttggg aaactttcgt gattcctgat gacatcggcg
    14341 gacgtttctc tgtattaact gctgttggtt tgttaccaat cgctgttagt ggtgcagata
    14401 tcgatgcatt gatgcaaggt gccgctgatg caagcaaagc atactcaagc gacaaactag
    14461 aagaaaacga agcttaccaa tacgcagcaa tgcgcaacat tctttaccgt aaaggcaaag
    14521 taactgagtt gttgatcaac tatgaaccag gaatgcaata cttctcagaa tggtggaaac
    14581 aattgtatgg tgaatctgaa ggaaaagacc aaaaaggcat ctatccttca agcgcaaact
    14641 tctcaactga tttgcactct ttaggacaat tcatccaaga aggacgtcgc aatatttttg
    14701 aaacagttgt gaaggttgaa aaacctcgta aatcaatcac aattcctgaa caagcagaag
    14761 atttagacgg tctaggctac ttgcaaggaa aagaagtgga cttcgtaaat acaaaagcat
    14821 ttgaaggaac attgcttgct catacagacg gtgacgtgcc taacttgcta gtcaaaattc
    14881 cagaaatgga tgcttataca ttgggttata caatgtactt ctttgaaatt gctgtaggta
    14941 tttcaggtta cttgaatgga atcaacccat tcgaccaacc aggtgtagaa gcttacaaga
    15001 aaaacatgtt cgcacttcta ggcaaaccag gctttgaaga tcttgctaaa gaattgaacg
    15061 aacgcctgta agataagggt ttttcgctag atttaaataa tcagtgatag aaacatgttc
    15121 gctttaaaat gaaaactggt acccatttag gtactcaaac atactgcatc gtttattcag
    15181 aataaggggc tgtgacaaga cgtttgtcac agtcccttgt ttgtatagaa atcgattgat
    15241 tgccttgctc tttagtttgt tttaggactt taagaacagt gaggctgact tcgaaggtta
    15301 attctgtttt tttgttttct agaatcagta agcaaaaaag cactcacttc gagtgctttt
    15361 ttgcgatata ttcttagctt aagataggcc taatttttct tttacagatg agggtacctt
    15421 atcgctggta attacttttt tctctatgat ccgcttttcg cttgctttca actgcaagat
    15481 cgttccttcg gggtaagcag aaccattggc agagtcacca gaagtaatga catcgaaatc
    15541 taactgtctt ttttcaccgt tttcagcata agcatctacg ttgtatttaa aacctcttcc
    15601 aattacttcc ccattatctg ctttgatatc agtttctgca ggcattggag ctttgactac
    15661 cgcataataa tctttaccta cataagtaga ctgatagtag tcccaacctt tccatcctat
    15721 aaaagcaatc aatatgataa cgatcaaccc taaaattttt ttcatgaaag atctcctttt
    15781 ttatttttat ta
//
LOCUS       DAZKCY010000059        15776 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000059 DAZKCY010000000
VERSION     DAZKCY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.059"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..550
                     /gene="hslV"
                     /locus_tag="ACG8XC_001699"
     CDS             2..550
                     /gene="hslV"
                     /locus_tag="ACG8XC_001699"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFP9191394.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            564..1964
                     /gene="hslU"
                     /locus_tag="ACG8XC_001700"
     CDS             564..1964
                     /gene="hslU"
                     /locus_tag="ACG8XC_001700"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFP9191395.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1982..2773
                     /gene="codY"
                     /locus_tag="ACG8XC_001701"
     CDS             1982..2773
                     /gene="codY"
                     /locus_tag="ACG8XC_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFP9191396.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2855..3730
                     /locus_tag="ACG8XC_001702"
     CDS             2855..3730
                     /locus_tag="ACG8XC_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP9191397.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="ACG8XC_001703"
     CDS             complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="ACG8XC_001703"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP9191398.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4627..5061
                     /locus_tag="ACG8XC_001704"
     CDS             4627..5061
                     /locus_tag="ACG8XC_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP9191399.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5227..7272
                     /gene="parE"
                     /locus_tag="ACG8XC_001705"
     CDS             5227..7272
                     /gene="parE"
                     /locus_tag="ACG8XC_001705"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP9191400.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7284..9734
                     /gene="parC"
                     /locus_tag="ACG8XC_001706"
     CDS             7284..9734
                     /gene="parC"
                     /locus_tag="ACG8XC_001706"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP9191401.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10011..12242
                     /gene="pflB"
                     /locus_tag="ACG8XC_001707"
     CDS             10011..12242
                     /gene="pflB"
                     /locus_tag="ACG8XC_001707"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9191402.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12486..13247
                     /gene="pflA"
                     /locus_tag="ACG8XC_001708"
     CDS             12486..13247
                     /gene="pflA"
                     /locus_tag="ACG8XC_001708"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFP9191403.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13332..14258
                     /locus_tag="ACG8XC_001709"
     CDS             13332..14258
                     /locus_tag="ACG8XC_001709"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFP9191404.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14440..15333
                     /locus_tag="ACG8XC_001710"
     CDS             14440..15333
                     /locus_tag="ACG8XC_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFP9191405.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15467..15592
                     /locus_tag="ACG8XC_001711"
     CDS             15467..15592
                     /locus_tag="ACG8XC_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFP9191406.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
BASE COUNT         5551 a         2701 c         3376 g         4148 t
ORIGIN      
        1 catggtcgaa tcaccatttc attcaaccac tatctgtgcc gtcgaaaaag acgggaaatt
       61 tgctatggct ggagatggcc aagtaacaat gggtgaatct gttgtcatga aaggcactgc
      121 aaagaaagta cgacgtatct acaatggaga agttgttgta ggtttcgccg gaagtgttgc
      181 agatgcgttt actttagaag aaaaatttga aggcaaacta aacgaatata acgggaatct
      241 acaacgcgca gctgtcgaat tagctcaaga atggcggaca cagcaatcta tgcaaaaact
      301 tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt cttgtttcag gtacaggaga
      361 agttatcaca ccagatgatg gtatcttagc tatcggatca ggagggaatt ttgcgttggc
      421 tgcagcccga gcaatgaaaa actatgctca aaacgacatg agtgcgaaag aaattgcaga
      481 aaatgcgtta aatattgccg cagatatctg tatctttact aatcataata ttatcgtaga
      541 agaactgtaa gaggtgagta gatatgtatg aattaaaaca aacaccaaga caaatcgtaa
      601 gtgaattaga tgaatatatc gtaggacagc acgcagcaaa aaaatctgtt gccgttgctt
      661 tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat gcaacaagat attacaccaa
      721 aaaatatgct gatgatcgga cctacagggg tcggtaaaac agaaatcgca cgacgtttgg
      781 caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac aaaatttact gaagtcggtt
      841 atgtcggaag agatgtagaa tcaatggttc gggatttagt agaaaatgcg atccaaatcg
      901 tagaaaaaga gcagtacagc cgtgtataca caagtgctaa aaaaaatgca gaaaaacgtt
      961 tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa acaatccact aatcaatttg
     1021 aacaaatgat gaatcttttt aatgctagtc agcaaaatac cgctgaaccg caagaagaag
     1081 tgacagaaga tattcgaatc aatcgccgta cagtcttgga acaactacaa aagggtttac
     1141 tggatagtcg tgaagtaacc attgaagttg aagaccaaaa gaaacctgca ccaatgatga
     1201 acaatggatt ggagcaaatg gggatcgacc taaatgaaac tctaggtgct ttgacaccga
     1261 agaaaaaagt ggaacggacg gtaacagtca aagaagcttt agagttattg atcaaggaag
     1321 aatcagcaaa attagtcaag gatgctgata tccatagtga ggcaatcaaa ttagcggaag
     1381 aaagtggaat tatttttatc gatgaaatcg ataagatcac ttctaaatca cagcaatctg
     1441 gagaagtatc cagagaaggc gtccaaagag acatattgcc aattgtcgaa ggttcacaag
     1501 taaacacaaa atacgggatc atccaaacag atcacgtcct attcattgct tcaggagctt
     1561 tccatttgtc taaaccaagt gatctaattc cagaacttca aggacgcttc ccgatacggg
     1621 tagaattaga tgatttgaca gctgaagatt ttgtgaagat tctgaccgaa ccaaataatg
     1681 ctttagtaaa acaatacgtc gcgttgatcg gtacagaaaa tgtgtcagtt acgtttacaa
     1741 aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt caaccgtgac acagataata
     1801 tcggcgcacg cagactgcat accattttgg aaaaactact agaagatcta ttatttgaag
     1861 caccagatat gcaaatggga gaaataacga tcacagaagc atatgtggat gacaaacttg
     1921 gctcaattgc ggcaaatgaa gatttaagtc gctatatttt ataaaaaatc ggaggacgac
     1981 aatgacagac ttgctaacga aaacccgcaa gataaacaca cttttgcagc aaaagaatac
     2041 ttttgacatg caagcagatt tgccttacga taaaatggct gtcacactag gtggtattct
     2101 tgacagcaac acctatatca taggaagtga cggagtatta ctaggttata atgaacgtca
     2161 cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag aaacaatttc ctgaatctta
     2221 tactgataca gtggcacagt tgatgaaaac tgaagctaat atcccaatta ctagtgatat
     2281 tactgcattt ccggtagagt caagagagaa atatccattt ggtctgacga ctgtcgttcc
     2341 gatctttgga gctggcgaac gtttgggtac gattatctta tctcgtatgg aacaatcgtt
     2401 tgcagatgac gatctagtct tagcagaata tggagctacg gttgtcggta tgcagatcct
     2461 ttatcagaag tctcgaagca tcgaagcaga tgtacggagt gcaacggctg tccaaatggc
     2521 aatcaacaca ttatcttata gcgaattgaa ggctgtacaa gctatctttg aagcattgga
     2581 tggagatgaa ggtcgtctga ctgcttctaa tattgccgat agtatcggca tcactcgttc
     2641 tgtgatcgtc aatgctttac gaaaattgga atcagctgga atcattgaat ctcgttcgtt
     2701 aggcatgaag ggaacttatt taaaagtatt gaattcccgt tttaaagacg agttagcaaa
     2761 acacagttat tagaagtaag gggactgggg cagatagaaa tgtttgttcc agccttccca
     2821 ttttaaaaag taagtcgaag ttggaggaat ggcaatgact gttattattc aaaatgacca
     2881 gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc agtctgaaat caaaagaaca
     2941 tggtttagag tatatctggc aaggtgatcc agcgtattgg ggcagacatg caccggtttt
     3001 attccctttt gttggacgtt taaaagatga tcagtatgtt tatcaaggga aaacatatca
     3061 aatggggcaa cacggatttg ctcgggatat ggattttgaa gtcattgaac atggacaaga
     3121 aatggctagt ttcatcttaa ggagtacgcc ggagacatta gaaaagtatc catttgattt
     3181 tgaattagtg atcagctatg aattaggagg agacggcatc acggttcatt atcaagtggc
     3241 aaataccggt gaaaaagaaa tgttcttttc tatcggtgga catcctgctt tcaatgtacc
     3301 tcttgaagaa ggattgtcat ttgaagattt ctatctggca ttttctccat tgaaatcaag
     3361 attacgccta ccattgaaag gcccattcat cgatatggat caaaagacgt taggtcagac
     3421 aaatacgaat ctggctttga atcgtgaact ttttgagcaa gatgccttgg tttttgaaac
     3481 aaggggtcta aatgcgtacg ccattcgaag tgaaaaatct tcccatagcg taactttaag
     3541 ctataaagat atgccttatg tagggatctg gtctccttat ccaaaagaag cgcctttcgt
     3601 ttgtatcgaa ccgtggtgcg gaatcgctga cacggtgaac agtagtggaa acttgatgga
     3661 aaaagtaggg atcaatcaac taggtgccca tgaaatcttc aagacaaaat attccatcac
     3721 agtgaagtaa gaggttgtga ccgagaagtt agcagggaac accgtttacc aaggtgtgta
     3781 aaaaagctat cctgtaagta ataagggagt gtgacatcga tttgtcacac tcccgtttta
     3841 tctctttgga atgaaaaagt atcaggattt tttcttgctg ctattcaaac caaacgggat
     3901 tcgactttca gtatggtttc tgatacgctt gatattgtct ttatgccgat aaaagataaa
     3961 ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg ggcttagcca aaatcacagg
     4021 agctacataa ggaaggacga tcgtagataa agtgatcaat actgcactga tcatactgct
     4081 caaactcacc atacttgtac aaaataaact aacgataaag atgatacaag aataaacaaa
     4141 aaatacagga ttaaatccta aaagcattcc tgcacttgtc gcaacagctt ttcctccttt
     4201 gaacccagca aaaatcggaa atgtatggcc aagaatggcg cataccccaa accaaagagg
     4261 gttaatccca gaaaggtgga agatcatcgg caaacaagta gccaatgtac cttttaaaat
     4321 atccataagc aggacaacaa tcccggctgt tttgcctaat acacgaaatg tattggtcgt
     4381 tccagtatta ccgctcccgt gttgtcggat atcttttttg aaaaacaatt tgccaatcca
     4441 gacaccggaa ggaatagaac ctaaaagata tgctaaaata agtaataaga tcaatttcat
     4501 tttttaccct ctttcaagtt gaaaactatc ctttatttta gcatgaatag gtaacaggaa
     4561 caattgaaaa aatgaaagat acaagtataa tagatggaat aaaaagaaaa gcaaggggtg
     4621 gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt tcaactattt ggaagaagca
     4681 aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac gaacaagtta catggttgca
     4741 caggtattgc aagacaatgg atataaaatc ataccagtaa atccattatt ggctggtgaa
     4801 gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa tcgatgatcc aatcgatatc
     4861 gtagatgtat ttagacggag tgaattttta cctgaaatag caggtgattt tttgaaaact
     4921 gatgctaaag tgttctgggc acaattaggg attgaaaaca aagaagcagc tgagatgcta
     4981 agaaaagcag gacgtacaga tatcgtaatg gatcgttgta ttaagattga acttggtaag
     5041 ttgaaagaaa accaaaaata aaaatgacca caaagaagag atgatcgata gaggggaatg
     5101 tcgaaaaagc ggttaggaga taattaaaat tgatttctcg tctaccctct tttttttcac
     5161 gaccttttct agtatgataa tcgtggtgcg gttttgccgc aaagtattat tgaaggagcg
     5221 tttgccttgg caaaaaaagt aaacaacgaa tacaatgact cgtccatcca agtattggaa
     5281 ggattagagg cagttagaaa aagaccaggg atgtacattg gatccactga cagccgaggc
     5341 ttgcatcatt tagtctatga aatcgtcgat aatgcagttg acgaagcatt atcaggttat
     5401 ggcagtgaga ttgatgtgac catccatgaa gacaatagta taactgttgc agatagcgga
     5461 cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa cagttgaagt gatttttact
     5521 gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca aaacttctgg tggactgcac
     5581 ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc taattgtaac gatcgttaga
     5641 gatggcgtag agtatcagca gaaatttaaa aacggcggta aacctgacgg tactttgaaa
     5701 aagataggaa aaacaaaaaa agcaaacggg acgactgtcc attttttacc agatgataca
     5761 atattcagta caacgaaatt ttcatatgaa attttagccg aacggttaag agaatctgcc
     5821 tttcttctaa aaggcgttaa aatctctctt tctgatcttc gtggagaaga accagtcaag
     5881 gaaatttttc attacgaaga aggaatcaaa gaatttgttg attatctgaa tgaagaaaaa
     5941 gatacattga caccagttgt ttatttttca ggagaaaaag aaggtatcga agtagaggtc
     6001 gcttatcaat acaatgacgg ttattcagaa aatgttttat cttttgtcaa taacgttcgg
     6061 acaaaagatg ggggaaccca tgaagctggt atgaaagcgg cgatgacgaa atcttataat
     6121 gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca agaatctgga aggtagtgat
     6181 ttcagagaag ggttagctgc tgtactttct atccgtgtac ctgaaaatct tctgcaattt
     6241 gaagggcaaa caaaagaaaa acttggtacg ccagttgctc gtaccgtagt agataacgtc
     6301 atcagtgaac aaatgggctt ttatttgcag gaaaatagcg aaatgagcca gatgttggtc
     6361 cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac gtaaagccag agaagaaagc
     6421 cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt caggaaaatt gacacccgca
     6481 caatcccgaa accctaagaa aaatgaattg tatttagtcg aaggggattc tgctggcgga
     6541 tctgctaaac aaggaagaga cagaaagttc caagcaattc tacctttacg aggaaaagtc
     6601 atcaataccg aaaaagctaa aatgcaagat atcttgaaaa atgaagaaat caatacgatg
     6661 atctatacga ttggtgcagg agtcggtcct gagttttcta tcgaagactg taattacgac
     6721 aaagtaatca tcatgaccga tgcggataca gacggcgccc atattcaagt attactgctt
     6781 actttctttt atcgttatat gaaaccgctg attgaagctg ggaaagtata tatcgcgtta
     6841 ccgcctttat ataaagtatc caaaggacaa ggaaagaagc aagtcattga atatgcgtgg
     6901 acagatgacg aattggcagc aatgattaaa aaagtcggca aaggatatat gctgcagcga
     6961 tataaaggac ttggagagat gaatgcggaa caattgtggg aaacaacgat ggatccgact
     7021 tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc aagctgaacg tcgagtaacc
     7081 acactgatgg gtgacaaagt agaacctcga agaaaatgga tcgaaaatca cgtgcaattt
     7141 acgctcgaag aagatggcag tatcttagat aaaaaagagg atactgaaat ctctccttca
     7201 gtttctaatg atttattaga tgaagaacga gcagataaaa atgaaaacaa tcagttattt
     7261 gaagtagaat aggaggtcac ctgatggaaa atcgacaaga agtacaagaa ttaactttag
     7321 aagaagtgat gggtgaccgc ttcggacgtt attccaaata tattatccaa gaacgtgcac
     7381 tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg gatattattt gccatgaaca
     7441 aagatggcaa tacgtatgac aaaggattcc gtaaatcagc aaaatctgtc ggaaatatca
     7501 tggggaatta tcatcctcat ggtgacatca gtatttatga agcgatggtc cgcatgagcc
     7561 aagactggaa gttgcgggaa gtactgatcg aaatgcacgg gaataacggg agtatggacg
     7621 gtgatccacc tgctgcgatg cgttatactg aagccagatt atccaagtta agtggagaga
     7681 tgctagcaga tatcgagaaa gaaacagtcg atctcgtgtg gaattttgat gatacagaaa
     7741 aagaaccaac agtccttcca gctcgatacc caaaccttct cgtcaatgga tcgacaggta
     7801 tatctgctgg atatgcaaca gaaatcccga ctcataactt agctgaagtg atcgatggca
     7861 caatctatat gatcgatcat ccacaagcga gccttgaaaa attgatggaa tatattcctg
     7921 gacctgactt tccaacagga gggatcttgc aaggaaaaga tgaaatcaaa aaagcttatg
     7981 aaacaggacg aggaaaagtt attcttcgtt cgaaaacaaa gatagaggca atgaagggta
     8041 ataaacagca aatcgtgatc acagaaatcc catatgaagt aaataaagcg accttggtga
     8101 aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg aatagctgaa gtacgagatg
     8161 aaagtgatag aacaggtctg caaattgtcg tcgaattgaa aaaagacgcc aatgctcaag
     8221 gaattttgaa ttatttgttc aaaaacactg agctacagat caattacaat ttcaatatgg
     8281 tcgctatcga ccatatgact ccgcatcaag ttggactgaa agacatttta agaagttata
     8341 tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt cgatttagcc aaagctcaaa
     8401 aaagacagca tatcgtagaa ggattgatga aagctttatc gattctggat gaagtaatcg
     8461 caacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa gaatttagtc gacgtctttc
     8521 aatttacaga agaacaagca gaagcaattg tcacattgca attataccgt ttgacgaaca
     8581 cggatatcac agagcttcaa aaggaatccg aaagcttgat tgcgcaaatc acagagttga
     8641 ataaaatcct ttcaaatgac aaagagcttt tctctgttat gaaaaaagaa ttgcgagaag
     8701 tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga agacgaaatc gaagaaatca
     8761 aaattgatac gcaagtctta gtggcacaag aagacgtaat tgtcagtgtg acgcgagaag
     8821 gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc caaaccagaa gaaatcggga
     8881 tgcgagaagg cgactacttg ctatactcag gtgaattgag tacattggac catgttcttc
     8941 tgatcacaaa taaagcaaat gtgatctacc gccctgtcca tgaactaccc gatttgaaat
     9001 ggaaagatgc tggtgagcac atttctcaaa caatctccaa tctgtctgta gacgaatcga
     9061 ttttagctgt tttcccatat caaaaaattc aagcagagaa aacatttgta ttcatcagca
     9121 agaatggatt gatcaagcaa acacgaatga cagagttcga accatggcgc acctataaga
     9181 gccgtccgct cagctgtatg aagctaaagg cggaagaaga tgaactgatt gctgtgtatc
     9241 tgaaagaaga tcagccaggc ttagatgttt tcttagtaac gcatcaagga atgggtttgc
     9301 gctatccttt aacagatgtt cccgttgttg gagcaaaggc agctggagtg aagtctatga
     9361 atttgaaaga tggagatagg attatcaacg ggctgcttgt gttagcagaa ggtgatacgc
     9421 cgatcgtaat cgttactcag cgaggagctg ttaaacgcat gcttgctcaa gaaatcagcc
     9481 aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga gctgaaaaaa aatccgcacc
     9541 gtattatcta tatgtcggaa ggaagatcaa aagaaataac cttgatgaat caaaaagggc
     9601 agcagttaac gattgaccca actgatttcc ctataggtga tcgaacatcg aatggatcat
     9661 ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt tatcgatgct cctgagattt
     9721 tcatcgagtc gtaactgttg tagtatttta atgaagagac gccatctgta ttaatacagg
     9781 tggcgtctct ttttcattca tttttaataa atccttatac gtttgcaaaa aaagatgaaa
     9841 gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt taaatatatg taaataaaac
     9901 ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa gatagggtgc tgtgttatac
     9961 taatcacgta aaaactgtta cataaaacag aaatttagga ggtccataca atggagcaat
    10021 ggaatggatt taaaggtacc aaatggaaag aaggcgtaga cactagagac tttattcaaa
    10081 ataactacac tgaatatcgc ggtgatgaca gctttttgga aggcccaaca gaagcaacag
    10141 aaaaattatg ggatgaattg caaaagttaa atgatatcca attcgagcgt aacggtgtct
    10201 atgatatgga cacagaaatc gtatcaacta ttacttcaca cgatgcaggc tatttggata
    10261 aagatttgga aaaaatcgtc ggtctacaga cagacaaacc tttgaaacaa gcattcatgc
    10321 cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag caatggctat acaccaaacg
    10381 atgaattgac acatattttc actgactggc gcaaaacaca taatcaaggt gtatttgacg
    10441 cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat cattacaggg cttccagacg
    10501 cttacggtcg cggacgtatc atcggtgact atcgtcgtgt ggcattgtat ggaatcgact
    10561 atttgatgga acaaaagaaa aaagatcatg atatgactgg ttatcataca atgagtgaag
    10621 aagtgattcg tttacgtgaa gaaatcactg aacaatatcg tgcattagcg caaatgaaag
    10681 aaatggctgc gaaatatggc tatgatattt ctaaaccagc tgcaaatgca aaagaagctg
    10741 tccaatggct atacttcgga tatttagcag caatcaaatc acaaaatggt gctgcgatgt
    10801 caatcggacg tatctcagca ttccttgata tctatatcca acgtgatttg gataatggag
    10861 taatcacaga aaaagaagca caagaattga tcgaccactt agtaatgaaa ttacgtatgg
    10921 tcaaattcgc gcgtacacct gaatataacc aattattctc aggattccct atctgggcta
    10981 ctttatccat tgcaggtatg ggattagatg gtcgttcatt agttacaaag aatgatttcc
    11041 gtctgcttca tacattaaca aatatgggac cttcaccaga accaaacttg actgttttgt
    11101 attctgaaca ccttccagaa ggattcagaa catatgctag cagaatcgct atcgaaagtt
    11161 cttcgattca attcgaaaat gataatcagc ttcgtgaaca atggggttct gatgattgtg
    11221 cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga catgcaattc tttggcgctc
    11281 gtgctaactt ggctaaaaca ctattatatg ccatcaatgg cggagtcgat gaagtgactg
    11341 gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga aatactagac tttgaagaag
    11401 tgaaacataa cttcatgaat atgatggatt ggttagcaga actatatgtc aatacgttga
    11461 acatcattca ttatatgcat gataaatatt cttatgaagc agctcagctg gctttgatgg
    11521 attctgactt gcaacgcaca tttgctacag gtattgctgg gatctctcat gcggcagaca
    11581 gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg taatgaaaaa ggaatagcag
    11641 ttgattttga agtggatggc gacttcccta aatacggaaa tgatgatgac cgtgcagatg
    11701 aattagtttc atggatcttg gaatacttca tgggacaaat caaacgtcat aaaacttacc
    11761 gcaactcaaa accaacaact tcattattga cgattacttc aaatgttgtt tacggtaaaa
    11821 atacaggaaa tactcctgat ggccgtgaag ctggaaaacc acttgcgcca ggagcaaatc
    11881 caagttacaa tgcggaacaa agtgggttat tagctagcct gaactcaaca gctaaaatga
    11941 attacggctg tgcattggat ggaatttcaa atacccaaac aatcaatcct tcaggtcttg
    12001 gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt cttagatgga tacttctcaa
    12061 aaggagctta tcatttgaat gtcaatgtat tcagtaatga tttattgcgt gatgcaatgg
    12121 aaaatccaat gaaatatccg aacttgacga tccgggtatc tggttatgca gttaaattcc
    12181 gtgacttgac aagagaacaa caattagatg tcttgatgag aacttcacac gacagaatgt
    12241 aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa ggacgttgaa tagccatcgc
    12301 atcaaaatcg aataatcagt atgcttattt gaaagaaacg atttacaggg gcaggacttg
    12361 tatgttccgt ccctgtaaat tttacacata acgaagcggc aattgcgaaa aattttgttc
    12421 agaaagcttc gcttcgacag ctaacttgta cttaaaatga aatcaaagaa aagatgtgaa
    12481 cttaaatgga agaaaaaaca atcggctatg ttcattctat cgaaactttc ggttcagtag
    12541 acggacctgg actgcgcttt gtcgtgttta tgcaaggatg ccgcatgcgt tgccaatttt
    12601 gtcacaatcc agatacttgg aatattggcg gagggaaaga atacacagca gatgaacttt
    12661 tagataaagc agaacgcttc agaccttact ggggagataa aggtggtatt actgttagtg
    12721 gtggagaacc tctattgcag atcgattttt tgatcgaact atttaaaaaa gcaaaagagc
    12781 ggaagatgca tactacattg gatacatgcg gaaaaccttt tacgtatgaa gaacctttct
    12841 tttcacgttt tcaagaattg atgaagtata cagatttact gttatttgac atcaaacaca
    12901 tcgataatga agagcataaa aaactaacgc attgggacaa tgaaaatatt ttagaaatgg
    12961 ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg ccatgtacta gttcctgagc
    13021 gaagtgatta tgatgaatat ttgatccgac tagacaactt tattcaaacc ttgtcaaatg
    13081 tagacagagt agagattctt ccttatcata cgatgggaaa atacaagtgg gaaacacttg
    13141 gtttgaaata tccattagaa gggatcgaac cacctacaaa agaacgtgtc gaaaatgcta
    13201 aacgattgct tcatacagaa gagtatacaa aatatttaac gcgataggtc gcatcggaaa
    13261 gaagataagt attgcttctt tccgtttttt tgctattatt aaataggaga aatcacaaag
    13321 gagcgaatga catgtcaaaa attttagttt ttggtcacca aaatccagat acagatgcaa
    13381 tcggggcagc cattgcgttt tcacacttgc aaaatgaatt aggtaaagaa acagaagcag
    13441 ttgctctagg tacacctaat gaagaaacac aatacgcatt agaccacttt cagctatctg
    13501 caccacgagt agtagagtca gctaaagcag aaaatgcaga tgtcatgctt gtggatcata
    13561 atgaattcca acaaagtatt tcagatatca gcgaagtaaa tattttagct gtagtagacc
    13621 accatcgtat tgcaaacttt gaaacagcta atccattgta ctatcgtgca gaaccagttg
    13681 gatgtacaag tacgattatt ttaaaactat ataaagaaaa ccaagtaaca attcctaaag
    13741 aaatcgcagg aatcatgctc tcagctatca tttcagatac attgttgttc aaatcaccaa
    13801 catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc agcaatcgct gaagtcgatc
    13861 taaacactta tggacttgaa atgttgaaag caggaacaaa tctaagtgat aaatcagctg
    13921 cggttcttct tgatttggat gcaaaaagtt tcccaatggg cagttccaac gttcgtatcg
    13981 ctcaagtcaa tacagtggac cttaatgaag tgatggatcg acaagcagag ctagaagcag
    14041 caatggaaga tgaaatgaaa gcaaatggct acgatctgtt cgtattagtt gtcacaaaca
    14101 tcttagacag tgattcagaa ttacttgttg ctggtaaagg aaaagaaaaa gtagaagaag
    14161 ctttccaaac aactttgacc aacaaccgtg ctttgcttaa aggtgtcgtt tcacgtaaaa
    14221 aacaagtcgt acctcaattg acggctgtat ttagctaatc aaatggtagt atgaagcatt
    14281 caaaatgaat ggataagcga ttgatataaa aagaaacata gaacatacta aaggccgaag
    14341 aaatggagga gaataacagt caattatgac tcttgttctt cctccttttt acgtaaaagc
    14401 cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta ttcaaaaaat
    14461 aaggaaaact tttatcaaaa actgaccgat gatccgcttt tttcttcatt gaccgattat
    14521 ctatatgaac atcgagaaca agaaacgatt ctaagagaat tgaaaaaaga attttcgcaa
    14581 aataaatttt cccattttct tgatttattg atcgatgcag gattaatcaa gagggaagaa
    14641 cgaagatatc atttgaactt tccaatattt gatcctaaag attacttgca acaagccact
    14701 tcggctgctg aaaccatagc tgaacaattg aagagactct ctgttgatga acaaaaatta
    14761 gcgatgggag aagttatttg ggcgtattgt ttcgaagatg aacggaaaga agcgtatttt
    14821 tatggagtaa gaaacagccg agagacagaa ttgctgagga caactgcagg aaatgagaag
    14881 taccgtttta tcacactttc ctctatagaa cacttcccgc ttactttggc taattacttt
    14941 tttgtacaga agaatcagct tcctgtaacg aaggcattca aagaattagc cgaacttatt
    15001 ggagacgtaa atgaatcgta cttttttgat caaatagaag ttatcgttga caggatacgt
    15061 aagaataaat ataaaaatcg ccggcctagt attttccatc agtctctact tgtgacgaat
    15121 actataaagg aagaagaatc attcacatta gaattaccta ttgtcgaaaa aaacaatttt
    15181 gagattgaat taccaacatt ggatccttca ttgacaatgg aggaaatggc ctttcttaaa
    15241 cgacaaatct tttctgagct gagcaagaaa tttataccgc atgcattttc ttatatcaaa
    15301 gaatatggaa cagttttagt gagtaaaacc taagagagca gtaagcaaaa attatactga
    15361 ctcttgcagg aggagccttt ctcttccttt atggttaaaa gaattatttg agtattttgc
    15421 ctatctacca cctattagat tcactacatg gtctggaaac aaaggaatgc aaaaaaatgg
    15481 tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa gttagatttt taaataatca
    15541 atattatgat tcagtcaagt atggcatttt atgggaagaa ctagctgatt agattaggaa
    15601 gtccatttga taatccagaa tcactaagaa acgaatggcg agtcattaat aatttatcca
    15661 atcttgggtt attcttttag attgaagtgt ttaaaactaa aaaagcattt atgaagtcgt
    15721 aatagtcagg cttcaaaatg ctttttattt tgtttgtttt ttctttgcca cataaa
//
LOCUS       DAZKCY010000060        15442 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000060 DAZKCY010000000
VERSION     DAZKCY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.060"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="ACG8XC_001712"
     CDS             complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="ACG8XC_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP9191407.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1197..3038)
                     /gene="dnaG"
                     /locus_tag="ACG8XC_001713"
     CDS             complement(1197..3038)
                     /gene="dnaG"
                     /locus_tag="ACG8XC_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9191408.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3220..5535)
                     /locus_tag="ACG8XC_001714"
     CDS             complement(3220..5535)
                     /locus_tag="ACG8XC_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9191409.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5720..6580)
                     /locus_tag="ACG8XC_001715"
     CDS             complement(5720..6580)
                     /locus_tag="ACG8XC_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFP9191410.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6639..7271)
                     /locus_tag="ACG8XC_001716"
     CDS             complement(6639..7271)
                     /locus_tag="ACG8XC_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFP9191411.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTNLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            7438..8199
                     /locus_tag="ACG8XC_001717"
     CDS             7438..8199
                     /locus_tag="ACG8XC_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9191412.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8225..10816
                     /gene="mprF"
                     /locus_tag="ACG8XC_001718"
     CDS             8225..10816
                     /gene="mprF"
                     /locus_tag="ACG8XC_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP9191413.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..12191)
                     /gene="tuf"
                     /locus_tag="ACG8XC_001719"
     CDS             complement(11004..12191)
                     /gene="tuf"
                     /locus_tag="ACG8XC_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9191414.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12559..12915
                     /locus_tag="ACG8XC_001720"
     CDS             12559..12915
                     /locus_tag="ACG8XC_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9191415.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(13156..14160)
                     /locus_tag="ACG8XC_001721"
     CDS             complement(13156..14160)
                     /locus_tag="ACG8XC_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFP9191416.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            14326..15177
                     /locus_tag="ACG8XC_001722"
     CDS             14326..15177
                     /locus_tag="ACG8XC_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9191417.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            15194..15439
                     /locus_tag="ACG8XC_001723"
     CDS             15194..15439
                     /locus_tag="ACG8XC_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191418.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         4482 a         3209 c         2730 g         5021 t
ORIGIN      
        1 tttttttctt ttaaaataaa ccaaacagtt ttactgattt ctcttctggt caatagtcca
       61 cgcttattct aagaaatctt ttaattgttt tgaacgagaa ggatggcgca attttcttaa
      121 tgctttagcc tcgatctgac gaatacgttc ccgagtaaca ccaaatactt tccctacttc
      181 ttccaatgtt ctcgtccggc catcatctaa accaaaacgt aagcgaagga cattctcttc
      241 ccgatcagtc aatgtatcta gtacatcttc caattgttct tttagtaact cataagctgc
      301 gtgttctgct ggacttgttg cttcctggtc ttctatgaaa tcacctaagt gagagtcatc
      361 ttcttcaccg attggcgttt caagggaaac aggttcttgc gcgattttca agatttctcg
      421 gactttttct gttggaagat ccatttccgc accgatttct tctggcgtag gttctcttcc
      481 aaggtcttgc agcaattgac gttggatacg tatcaattta ttaattgttt caaccatatg
      541 aactggaata cggatcgtac gtgcttggtc agcgattgct cgagtgatgg cttgacgaat
      601 ccaccatgta gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc
      661 cttcatcagt cccatgtttc cttcttggat caagtcaagg aactgcatgc ctcgtcctac
      721 ataacgttta gcgatactta ctactaaacg aaggtttgct tctgccagac gctgtttcgc
      781 ttcttggtcg ccttcttcga ttttcaatgc taacgctact tcttcttccg cagttaaaag
      841 ggagacacgg ccaatttctt tcagatacat acgaaccggg tcattgattt ttacaccggt
      901 tggcgcagaa agatcttctg tttttgcttg ttcagctttt ttctcagcac tcttcaagct
      961 gtgaattgat ggatcaccat tttcatccac cacactgata cctgcgtctt caatcttttg
     1021 gatcaatttg tccatttcat ctgcattcaa agtgaatggt gtagcaagct gattggataa
     1081 atcatcatat acaacttgtc gtttgggttt gttttctttg atgaaagctg ctactgctgc
     1141 ttcatacgtt tttttatctg ttccatttgc cataaaagaa ggcccccctt gagtagctat
     1201 ttggcttgtt ttaactgttt cgttaaattg atgatttcaa tcgtcaattc aagctcaagc
     1261 tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt tttttaatgt tatttcatca
     1321 gctaagccag atttgctgat cacatctagc aagtcttgga tttctcgtgg cgaactttct
     1381 tctgccaccg gtaaatattc gatttccacc accaaccgcc ttaaattttc atcttgcaaa
     1441 aagtctaaaa actctgcaag gttgaattca taatgttgtg agacatatgc atcgaataaa
     1501 aaataaactt ctgcatatgc atcatgaata aactgaaatt ctcgttcttt taaaatatta
     1561 cggacagaaa cttctttaaa tgcacgatat aatagaatct gctctgcctt ttctacttgc
     1621 gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc
     1681 tgaggttctt gacgattttg ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc
     1741 gtctctctgc cgatctgaaa ctctgttgac agttgagata gatacatatc ttgttcgatt
     1801 ggtgaaacaa ctcggctaag ttcaactagt aattcttcga ggtattcgat cttgtctttt
     1861 tcattatcta aatttttccc ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg
     1921 cgcccatgag ccactaattc ttggaaagat gcttctccat acttacggag atattcatca
     1981 ggatcaagct tttctggaac gactacgaca gaaagtgcta atcgactgtt ttgccgaagc
     2041 aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc
     2101 aattctttcg tcagcttttc catttgttgg atctgttgag ctgttagact tgtccccata
     2161 gaagctaatc cagttttgat accagcctgc catgcagcaa tcacatccat aaacccttcg
     2221 aacaacaaaa tttctccgtt tttccggata tcgcttcttg ctttatcaag attaaataaa
     2281 acttgtcttt tattgaacag ttctgtttct ggactattca agtatttggg ctgatctttt
     2341 tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa
     2401 ttacgaagtg gaaacatcac tctttgataa aaacgatcag acatacggcc atcatcatgc
     2461 gtgacaaaca atccagagcg ttctaacaat tcatttgaaa ttgattcatt cttgaatact
     2521 tgctgaagaa aagaacgctc attaggtgca aaacctatgt tgaattcttc aattaattct
     2581 aaagacaacc cacgactttg caaatattct aaagctggct gtccagcttt tgtatgcaat
     2641 agcatgtgat gatagatttc cgctgctttt tcatgagcag caatcagttt ccccgtagca
     2701 gattcaggat tttggacagc tatagtacgc tgacgccatt gatcttctaa cggtatctgt
     2761 tcgaagtctg ccacttttac cactgcttct ggaaaactta gaccttctag ttcttgaaga
     2821 aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct
     2881 gcaacagaaa aagacggtgt acgttcttca tgaaaaggac acagccccaa gtaattttta
     2941 ccagactttt tcaactggac atattgtcca atcacctcaa cgatattcgt ttgactgcgt
     3001 actgtttcga taacctcttg aggaatccgt tgagccattt tttcaccccc tgctctattg
     3061 atatcttttg ggtttaacag aagatatttc aggagaataa ccggtaaaaa atcgcactaa
     3121 ctgaaatcag tgcgatttta aaggacacaa ttatacatgt ttgattgtat catatttcat
     3181 tgctttttca agtataacac atttctatct cattaagcat caatttgcta ctcttttcag
     3241 taactgattt tgaatatcag ccttccagta caatactcga agaacaatat ttacgagcat
     3301 gaatacaaag aagtatccta cgctctctcc caaccctaag tttacttcca aaattccatg
     3361 gaacagcatt gcagctacat atatccctac gatcgtcgtt actgctagga agatgcgcaa
     3421 tgggttgaag ggataacaag cttttacaac tgccatgcaa ctgatcccaa tcagcaagta
     3481 atacatcaaa gtagtagttt cagcatttcc ccatgcgaat gtttttccaa gtagatacac
     3541 cgcaatgata ttcactacta caagcaaggc atttggtaaa gcattaataa gtgctgtagg
     3601 caaataacgg taagtgattt ttcttttatc ttgttcaaaa gataaaaaga aggaaggata
     3661 cccttcaatc gctaaatcaa tcagcgtgat ttggataggg ataaacggaa atccaattgc
     3721 tgtaacggca catataatcg ataaaataaa tgaataaatc gttttgataa aaaagatgcc
     3781 tgagactttc gtcacattgt tgaccactcg gcgtccttca aacaggactt ctggcaatgt
     3841 agtaaaatca gagtccaaca ataccagatt cgcaatctga cgtgttgctc catcaccttc
     3901 tgccatagca atgctgcaat ctgcttctct taacgccaaa acatcattca caccgtcgcc
     3961 tgtcattgca accgttctgc cgctttcttt caattcattt accagcaatt tcttttgctg
     4021 cggactcact cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt ccatttcttc
     4081 agtaatttga gaaagatcga catacgcatc ataattcggt aatcctgcac gtctagcgat
     4141 attggagaca gtaaaaggat tgtctccaga aatcactttc agatccacac cttcctcatg
     4201 aagataagct agtgtttctt gagcgttttt tcggattgga tcatcgattt caaacagagc
     4261 aaccggctca acaaatgctg gtttttcttc gttcaattgc tgatttggag cgatccccaa
     4321 cataagtacg cgatagccat tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc
     4381 ttccaccaaa cgttcaggcg ctcctacgta cacgactccc agttctggaa attccatcgc
     4441 gccccatttg cgatcagaag aaaatgcgac tttgtttgtc acatcatacc gattagaaag
     4501 ctcaaaataa tctctcatag cccgcatagt gatgttgtta tcactgcttt ctgccagata
     4561 cgtccccata atttctggga tggctttctc gtacagcgga tgtagtgctt ctacccgctg
     4621 gatactcatt ttcccttcag taatggtccc tgttttatcc aagcataatg tatcgacatg
     4681 ggccagcgtt tcaattgaat acatgtcttg aacaaggatt cgtttttttg ccagtttggt
     4741 tacccctgtc gttaatgcaa tacttattag caataccaaa ccctttggaa gcatgcctaa
     4801 caaagctgct gcagatgcaa taacagaaat tttgatagaa gcatcgcgga tgaacagtgc
     4861 ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt
     4921 gcgaatcgaa tctacaagtt cagaacgaat agctttgtga gtctttgcct cgctagtcaa
     4981 gcggacagca taattatctg cacctactcg gataacttca gccatgacag aaccgcttgt
     5041 caaaaagctt ccagaaagca attcagctcc agtttctttg acaatcaaat cagattcgcc
     5101 tgtcaaaaga gcttcatttg cttctgcccg tccagatata acctgtaaat cggctggtat
     5161 ctgttctcct gctccgattt ttaccaaatc tcctaatacc agctctttag tagaaatcga
     5221 ttgttcttta ccgtcccgga tcactagtac ttctgattgg gaaacaatcg ataatttccg
     5281 tacaagattt cttgcatgta tttcttgata aattccaata acgatattga cgacaataat
     5341 tgctaaatac gccatgttgg aatacgcacc tacaaataac agacaaatcc caataccaaa
     5401 gttcagaaaa ttgaataacg tcaatatatt atcgctgaat atttgttttg tactttttga
     5461 tgcatcttcc tggtagtcat tttgctggcc agaagccatt ttctgaacta cctcttgctg
     5521 tgtaagtcca ttcattttct ttttctccat tttccgccaa ctttcaaaaa taatcacgtg
     5581 tttaagtcta gcgtgttcca agattaaaaa caacttgttt ttataaaatt tatagaaaaa
     5641 cacacgaatg attttacaaa aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt
     5701 ttttattatc aatctatctt tacttatatt gttcctccaa acggctcatc caccaaccta
     5761 gtgctgtacc ggctataaaa agaacaggac aagctagtac cccagcaact cccaatcccg
     5821 cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac tacacctaaa atgaaatgga
     5881 atagcttgct atatgccttt gagaaaatgt aatccatgat ttttgacaat ccaagaacac
     5941 aaagaatccc accaattccg atcggcacaa tcacttcaag tcgtacttct ttaaatccgt
     6001 cagccatcgc tttatacaat cccatataca ccaaaaaatt agaagggctc aatcctggga
     6061 cgatcatacc caaaccaatc aatccgccgg caatcagcca tgttccaaaa ttctgatcga
     6121 cttgcccgtc aaataattga gcgccgtata ataaaaacaa ataagccaga acaaaagtaa
     6181 caaccatgat gatcacatca gaagtatctc ttcctttttt ccctgcttct ttccataacg
     6241 caggaacagt tccagcgata catccgacaa aaaaccataa tatcgttgtt tcatacgcac
     6301 ctaataaaaa actgactaaa aaagaaagta gaaagatgcc tgtcactccg cctagaccaa
     6361 caggcagaaa aaacaaaaca ttttctttga aattttttgt aaggtgggct aaaaaagaaa
     6421 tcagccgctc atacatgcca aagacagctg ctaatgcacc accgctgact cctggcaaga
     6481 taaatcctga accgataaac attcccttca caaaccgtaa catccagtct ttggttgtgt
     6541 accccacttc ttgattttcc attttttgcc ttttttccac tatttgcgca ctccttcttt
     6601 catttaattt tattcatgat tgaacatttc ctgctatttt acttaaagac tttttcatag
     6661 gtctctatta ttttttcatt cattttgaca atatcaggat aaaacatgtt acctccatta
     6721 tgatagagat agccgccatt gtaaagcaag gactggatcc gccaatatcg gtattgtttc
     6781 ccttcctcat ttcctaaaag tgggctcagc acatctttag aatacgcttc tgctaattct
     6841 actgtgtttt ctccgccatg attttttaca taggcaatat aatctttccc aaaattatat
     6901 gcttggacag ctgtccataa atcgcatcct tgtttctttg cttctactat catttccgcc
     6961 aaatatttga caccttgatc aatactgtca gctggattat caatgatctc agcttctcca
     7021 tgcagacttt ccgagctctg catgagattt gtcccttttc cttttgattc ggtcaatatt
     7081 atcgccttga tcaaattctc gtagttttcc atatgataat tttttgcagc tgttgttatt
     7141 tctatttgat agctttccac tcgttttacc gaacgataag ttcttcctcc taaaaacaac
     7201 accgcaccaa taaccgtcag aatgatcatg taaaaaagaa tacggataat agataatcgt
     7261 tttcgcttca tttcatcacc tagttatttt gacatctttt tttatcttac tatttcttta
     7321 tcttcttggc tatttcttcc ttgatttctg tgtacgcctt aagaaaaaat gaattttcct
     7381 gtattttttg acatcttacg aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg
     7441 actgatataa tcgttatact ttacacagta ggtgattatg tgaaaaaaga aaagaaatat
     7501 gcaaaaatgc cagatgggag cgaaatttac tatgaaaaga gcggtcaagg cttccctctc
     7561 tttttactgc atggcaatga cggcagcggc cgcttttttt cagaacaggt ccctgtactc
     7621 gaacgctact ataccgtgta tctagtagat agccgaggac atggtcgttc gacaaacgaa
     7681 gcatctatgt tgaattttca gttaatggcc gaggatctga atacgatcat gttgctggaa
     7741 aaaatcgatc aggcagattt tcttggattt agtgatggag caaatctagc actggtattt
     7801 gccagcagct tccctaaaaa agttcatcga ttgattttaa attctggaaa cacgcttgtt
     7861 aaaggcgttc gtttttctgc cagagtcatc agtaatattc attatgcatg ggtatggtta
     7921 ctctctttat ttcgcccaag tcttcgcaaa aatcttttag ttatcaaact tttgctgcat
     7981 gacatcgggc ttacagaaaa tgatttaaaa aaaatcaact cccccacttt gatcatcgtg
     8041 ggaaaaaaag atgtaatcaa gctaaaacat tctttgtata tagctaaaac tataccaaaa
     8101 gcttcctttg tgcttgtgaa agaacaagga catgaattag ctagaaaaga tcctgaacgc
     8161 ttcaatcgag aagttcttca atttctttct gaaacataac tattaggaat agaaggtgat
     8221 ttatttgtta aaaaatacca tacaatggct gaaaaaccac ctaggactgt tcaaaacaat
     8281 ctttttgatt tctgtcattg tcattattgt cggtgaattg atgtccatcg gtaaaactct
     8341 atctatccag caattaggag aaacgtttgc cactatacct gtatggaaaa gcggactaat
     8401 gctattgatt ggtcttatct ctgtgttacc tatggttggt tatgatatta ttttgaatcg
     8461 gatgcttgaa caaaagcaga atcctcgtta cctgtttgag acaagctggc tgatcaatac
     8521 gatcaacaat attgccggtt tcggcgggtt tgttagtgtg gggcttcgct ctgaactata
     8581 tggacaaaag aaagagagca gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt
     8641 gatggctgga ctttctattt acagtctgct ttctttttta cttgtagtct tcacgccagt
     8701 tgctcctttt ttgaagcaat attggatatg gttgatcggc ggcggtctat attttcctgt
     8761 cgtcttttta tttactactt tcaaaaagaa aggattcctt ggtggtacct cacttaaaac
     8821 tcaaggacag ctattgctag tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct
     8881 ttctgttggt tatttaatgg aaatccggat tgatctgtgg caaacaattc cattattcat
     8941 tgctgcttca gtgataggaa tcgtgtctat gattccagga gaaattggga gtttcgatgt
     9001 aatgatgatt atcgggttgt ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat
     9061 cttgctctat cgcctgttct actacatcat tccgtttttg attggaatcg tctttttctt
     9121 caaaaatatc ggttccactt ttgatcagcg ttattctggt atccctaaac aattagcaac
     9181 agaaattgcg cataaaatcg tggttgtctt attatacttt tctggaatca tgctagtact
     9241 ttctgccact atcccgcaag cttttacaga gtttcgctgg cttcatagtt tgaacccatt
     9301 gaaatttcat tttattatcc aatttcctag tatactattg ggatttcttt taatcattat
     9361 gggcagaggg atcgcagcac gtgtgaaacg agcatatttg cctacaatct tcttgattgt
     9421 tctagcttta ctttatgttt tattaagtga ttttagtttc acaccggtgg ttttcttgtc
     9481 tattctgctt ctgatcattc ttgcttctaa aaacgaatta tttcgggaac aattgatcta
     9541 ttcatgggag tggcgtacga tcgatggaat cattattggt gcactgtcct tgctttacat
     9601 cgttattggg gtatacaatc tgccagattt ccctcatcgg cgtcaccatt ttatttcctt
     9661 tttcttattt ccttctgaaa agatttggtt ttcgggattg ttggccatca tcgctgttag
     9721 tttcatgatc gttctatttg ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc
     9781 ctttaatgaa gaaaaggccc tgaagatcct tacaacttat ggaggaaact ccgatagtca
     9841 attgatcttc ttaaaagata agcggatgtt tgcctatgaa aaagacggtg aaccaactgt
     9901 tcttctccaa ttcgcttgct tcaataataa atgtattgta atgggtgatc cttctggtaa
     9961 gaaagaagat tttccagaag caatcgaagc ttttatcgaa gaaacagatc gactatgcta
    10021 tttaccagtt ttctacgaaa caagcgaaga gattgttatg attttgcatg aattcggcta
    10081 cgatttcatt aagatggggg aagaagctta tgtagacctg aatagtttta caacttcagg
    10141 gaaaaaaatg aagggcactc gtgctgtctt gaatcggatc gaacgagaag gatttacatt
    10201 tgatgtctta caaccaccat tttctactga gcaaatgagt actttcaaaa atatctctga
    10261 taattggttg ggttcacgaa aggaaaaagg gttttcttta ggctttttct ctgaagatta
    10321 tctacaaaga gcgcctattg cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc
    10381 aacgatcatg cctacttaca cgaacaataa agtaggaacg attgatctga tgcgatatga
    10441 ttctaaaaaa gcaccttctg gcagtatgga tttcttgttc ttgcatttgt ttgcctatat
    10501 gaaagaagaa catatccaat ggttcaattt agggatggcc cctctttcaa atgtcggaac
    10561 ttccagaaag agttttttac aggaaaggat tgcatacttg gtttacgagt ttggttctca
    10621 tttttattct ttccatggat taagagaata taaaagcaag tacgctacaa actgggtctc
    10681 acgttatact ctatattcta gagatagttg gattgcatat gttatgattg ctttgctgat
    10741 tattgataat gcgccagttg agcagacaca aggtagaata agtggtttga aaaaatggat
    10801 acgaagaaag tattaaaaac tgcataccga tagatgaaca ctctcatgat gaataaaaga
    10861 gactgaagta aaacgaaagt tcatgcttct acaatctctt cttatacaat tgaagaacaa
    10921 agagcggtcc aaacagaaat cctgtttgga ccgctctttt attttaaaca tttttcatta
    10981 ccaaaatatt cgagtcgcgt ttcttatgct tcgatctctg taacgatacc tgaacctact
    11041 gtccgtccgc cttcacgaat tgagaatgtc gttccgtttt caacagcgat tggatggatc
    11101 aagtcaacat caatcgtcac gttgtcacca ggcatgacca tttctgtatc ttctggcaat
    11161 gtgattgttc ctgtaacatc agttgtgcgg aagtagaact gtggacgata gttgttgaag
    11221 aatggagtat gacgtccacc ttcttctttt gtcaacacgt acacttctgc tttgaatttt
    11281 gtatgtggag taatggaacc tggtttagca agtacttgtc cacgttcgat atcgtcacgt
    11341 tggatccccc gtaacaacac gcctacgtta tccccagctt ccccgtaatc taacgtttta
    11401 cggaacattt ctacacctgt tacaactgct ttttgtgttt caggtttgat ccctacgatc
    11461 tctacctcat caccgacacg aacagcacca cgatcaatac gacctgaggc aacagttcct
    11521 cgacctgtaa ttgagaagac gtcttccact ggcaatagta atggtttatc tgtatcacgt
    11581 tctggtgttg ggatatattc atctactgta tccatcaatt ccataatagc agcttcagca
    11641 tctggatcgc cttgcaatgc ttttaatgcg gaacctttga tcacaggagt gtcatcgcct
    11701 gggaaaccat attcgctcaa taactcgcga acttccattt ctaccaaatc gatcaattct
    11761 tcatcatcga ctaaatcaac tttgttcaag aaaacaatca ggtattttac accaacttgg
    11821 cgtgacaata aaatgtgttc acgtgtttga ggcatcggac cgtcagttgc agaaacaact
    11881 aagattgcac catccatttg tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt
    11941 cctggtgcat caatatgcgc gtagtggcgt ttttctgttt catattctac gtgtgcggta
    12001 ttgatcgtaa tcccacgttc acgttcttct ggagctgcat caatactagc atagtcttga
    12061 ggatttgcta ggcctttttt gcctaatact gtagtgatcg cagcagttaa agtagttttc
    12121 ccatggtcaa cgtgtccaat cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt
    12181 tcttttgcca taatttacaa acctcctaaa taatatagat cactgaaaaa tttcagctct
    12241 caccttttta ttatagtctt tttttgacta aatcaaaaag aatacgtccg acttactttt
    12301 tgtaagattc gaactaagct tattatacgt caatattggt caaagtcaac taagagcact
    12361 cgtctgtctt atacgcgaaa aaagtacctt ttataaataa aaaaatgtgt tttttgagaa
    12421 aaacttcaag atagtcctta actttctttt gaagcaataa gaacaaaatg aggaaaagtt
    12481 taaaattcag ctgttgccaa caactttcaa tagacgaaat cttctatttc gttaataata
    12541 aggaaagagg tgaagtacat gaaaaaatta attggcgtac ttgtcgtttt aggcatattt
    12601 ctcggcggaa gttttgcagc ttatcattat ttttacggcg gcgaagctta ttatacaaaa
    12661 atcacgacga atggcgaaat gtcagctacc caagcagata acggagagaa attcacgact
    12721 tatacctaca agcaagcggc ttataataaa gatggtgaaa aaaaacaggt gacgcttcgc
    12781 gaagaaagag aacatccatt aaagatgaat gcgtatctta aactcaaagt caatcctcgt
    12841 aaaggtgtat taagctggga agaaataaag gcttctgaag tgcctaaaaa tgcggcagaa
    12901 aaaatcgata attagtaaat ggtcttaaaa ttttattaga taaaaagagg ctgggacaga
    12961 agcgtttaac tccaagaaat aagaaggagt tgcttctgct tccgccgttt atacgtgttt
    13021 agagcgtgag acaaaagtgt tctttacttt tgtctcacgc tctttttttg ttatattcgt
    13081 cttttccgat ccttctcttg acacaattca tttaactgat cactggtact tgaacaccta
    13141 atattctagt catttttata aacgattcaa tacttcatcc acatttttga tcatcacgga
    13201 tatcgtacca tccggattat tcacaaattc aatcaaatct gggttgcgat atacatctac
    13261 tggtacgatc agttcgatcc cgtttgatag cttgaatttt tgttttccaa attttttctc
    13321 agaaatttct tgtacttctt ttacaggagg tgcttctggg acaaatcctt gctcttgtac
    13381 ttcttcttta aaagccattt ttgccgtgat gttttccttg aagacttttt ccgcaatcat
    13441 ctcatgatcc aaccgacctt tttcttctat cgtatcatag acagcttctt tcaaatcagc
    13501 catgatatca aattcttctg tctcaaattt ttttccaaga tgcttggcta ctttttggat
    13561 cacttttaca ttttcttcta aagaaggtgc aggaacactt tcgatcactc ttccagaaaa
    13621 ataaagctct tttttgccag aaaactcata ttttttttca attaattcgt aggtccaatc
    13681 agtcaaattc acagcgaatg cttcatctgc tttttgtgtt ttcgccccta gaattgctcg
    13741 attcaaaatc aagcggttgt cgatccctgc ttcgtctgct tcaacaaagt gagtgtaagc
    13801 ctcacgataa ttgactttca ataaagataa gtacatcact gtatctaatt catacaagac
    13861 aacaaataca tctgcactag gtgcttcttc actttcttgg tagatatcga accaccgctg
    13921 aacgattgct tcacttgctt ccacaaaatt ttcttgagcg attttaagtg attcaacgat
    13981 catgctttct tctgctagtt gtccagtttt tgtttgagca gaagagattt tttggatctt
    14041 cttttgaaga aaatcacgaa tatattcttt tgtcagatcc agctcttttt gtgaaaagac
    14101 tggagaacca gtttctctgt caataatatg cagaattgct ttttttaaat agatatccat
    14161 tttctcattc ccttctgtct tagtttacgg aaaaaaaatc aaaaatacca gaatcaaaat
    14221 cattttcttt taaacaaaaa tttcaagagt agaaacaaaa tggattcttt ttttcgaaaa
    14281 atgcgctaca cttatagttg aacgaataaa aaaggagctg aaagtatgaa ttcattaaca
    14341 agcacttatc gtctatcaaa tggttacgaa atcccggtag taggattcgg cacatggcaa
    14401 acacctgatg gggatgtcgc tgtttcctct gttaaagaag cgctggctgc aggttatcgc
    14461 catatcgata ctgcccaagg atacaagaat gaagaaagcg tcggccaagc gattaaggaa
    14521 agtggaattc ctagagaaga gatattcttg acaacaaaat tatggaatgc aaaccacagc
    14581 tatgaacttg tgatgtcttc ttttgaagaa tcattgaaga agctccaaac agattattta
    14641 gatctgtttt tgatccactg gccaaaccct gttgcttttc gtgataattg ggaacaagca
    14701 aatgcagata catggcgagc atttgaagaa ctgtatgaag caggtaaaat caaagcaatc
    14761 ggtgtgagca atttcttacc ccaccatcta gatacactag caaaaacagc aaaaattatg
    14821 ccgatggtca atcaagtttt ccttgcacca ggcgaattac agacaactgt agttgagtat
    14881 gcaaaaaaac atgatatgat cctcgaagca tatagtccat taggcactgg taaaatcttc
    14941 gatgtgcctg aaatgaaaca aatcgctgaa gcacatgaca agacgattgc acaagttgct
    15001 ttgcgttggt cgctgcaaca cgaattcctt cctcttccta agtcagtgac accaagcaga
    15061 atcaaagaaa atactgaatt atttgatttt gaattaacag aagaagaaat gaaacaaatc
    15121 gatcagttag atggtgtagt cggcaaagca aaagacccag atactaccca attttaagtc
    15181 gaaaggtgat tttatggatc caatcgaacg cttgaattct ctctcggaag aagttaccca
    15241 gacttttcat tccgattttg tctttttgat cgatgcagag aaaatccaac attttcctgc
    15301 aagaaactgg acacatgatc aaatcatcga agaactgaaa aaacggttcg atcattcgct
    15361 aatggtcaaa ccctggcacg aacatgaagt catttattca cctgaactac ctgtttttgc
    15421 cttgataccg aaaaaataga aa
//
LOCUS       DAZKCY010000061        15291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000061 DAZKCY010000000
VERSION     DAZKCY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.061"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1123
                     /locus_tag="ACG8XC_001724"
     CDS             5..1123
                     /locus_tag="ACG8XC_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9191419.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1251..2627
                     /locus_tag="ACG8XC_001725"
     CDS             1251..2627
                     /locus_tag="ACG8XC_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9191420.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3028..4608
                     /locus_tag="ACG8XC_001726"
     CDS             3028..4608
                     /locus_tag="ACG8XC_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP9191421.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4806..5030
                     /locus_tag="ACG8XC_001727"
     CDS             4806..5030
                     /locus_tag="ACG8XC_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFP9191422.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(5235..5555)
                     /locus_tag="ACG8XC_001728"
     CDS             complement(5235..5555)
                     /locus_tag="ACG8XC_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFP9191423.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5703..7937)
                     /locus_tag="ACG8XC_001729"
     CDS             complement(5703..7937)
                     /locus_tag="ACG8XC_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9191424.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8416..8682
                     /locus_tag="ACG8XC_001730"
     CDS             8416..8682
                     /locus_tag="ACG8XC_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFP9191425.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            8682..10409
                     /gene="ptsP"
                     /locus_tag="ACG8XC_001731"
     CDS             8682..10409
                     /gene="ptsP"
                     /locus_tag="ACG8XC_001731"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9191426.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            10573..11619
                     /locus_tag="ACG8XC_001732"
     CDS             10573..11619
                     /locus_tag="ACG8XC_001732"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9191427.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            11636..12859
                     /locus_tag="ACG8XC_001733"
     CDS             11636..12859
                     /locus_tag="ACG8XC_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9191428.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            12948..13835
                     /locus_tag="ACG8XC_001734"
     CDS             12948..13835
                     /locus_tag="ACG8XC_001734"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9191429.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            13921..14151
                     /locus_tag="ACG8XC_001735"
     CDS             13921..14151
                     /locus_tag="ACG8XC_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFP9191430.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(14208..15173)
                     /locus_tag="ACG8XC_001736"
     CDS             complement(14208..15173)
                     /locus_tag="ACG8XC_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9191431.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         4958 a         2630 c         3119 g         4584 t
ORIGIN      
        1 tctattgttt gaaaaactta gacagtctaa actaatgttt tggtcgcttg aattattgat
       61 catagctgca ttgatcatga tttcatcaga aataaacttt attttcgaac cgatcgggac
      121 atttttttcc acgttatttg cacctgtact gattgcaggc tttctttact atttattgaa
      181 tcctattgtc aagctgttga tgaaagcaaa aatcaaaaga atatgggctg tcgcaattgt
      241 gctgctcctt ttagttgcag caattatttg gattttactt agtgtcatcc caagtctagt
      301 acagcaaatt tcctcattgg catctaatat gcctgatttc atcaaacagg tagaaagctg
      361 gttaaaagag gtagccgaat tgcctttgtt caaggaagta gatatcaata aatattttga
      421 gcaattagac atttcttacg ggacaatcat tcagcaattc cttagtggag tatccaatag
      481 tttaggttcg atcgttggaa cgatcgcttc ggcaaccgta gtaatcatta cagcaccatt
      541 catcttgttc tatatgttga aagacggaga aaaactagtg ccaaacatcc aacagttttt
      601 cccagaaaaa agaagaaacc aaattgtgga gttattgggg caactgaata aaacattggc
      661 taattacatt agcggacaag caatcgaatg tttattcgtt ggtaccttca catttttagg
      721 atatatggta attggtgtgg attatgcttt cttatttggg gtcattgcgg gattgacaaa
      781 cctaattcca tatttaggac cttatctagg actagcacct gcatttctag taacagtctt
      841 caatgatccg gtaaaagcat tactttgttg tgtggttgtg ctaatcgtcc aacagttgga
      901 tgggaatatc atttatccta atgtaattgg gaaatcactg aaaatccatc cattaacgat
      961 cattatcgtt cttctagtag ctggaaatat tgccggattg cttggtatct tcttaggtgt
     1021 tcctttttac gcaatctgca agacgatcat ttcctatata atcaaaatcg tcaaagaaga
     1081 caagcaaaat gaaaacaaga agaaaattgc tacgacctcg taggtagttt tagtacactt
     1141 gtttttattg caaagaaaat tgctttgtga taccatttta acgtggctat agtgccacgt
     1201 ttttttctat cgtaaactaa aaattaattt tataaggaga gaatgcgatt atgaatgctg
     1261 accctgagag tcagtcgctg ttagcgcaga ttttattatt gatcgttttg acattgatca
     1321 atgctttttt agcagcttcc gaaattgctg ttgtttcgat caataagaat cgaatcgagc
     1381 aaaaagctga tgaaggagat gtgaaatctc aaaagctttt aaaaatattg cagaatccaa
     1441 ataatttcct atcgacaatc caagtaggaa tcactttggt caacattttg tctggtgctt
     1501 cactagctaa tactttatcg gaacggttag ctccggtatt aggtggcggc acagcggcaa
     1561 aaaatattgc aaatatcatt gtgctggcta ttttgaccta tgtatcgatt gtttttggag
     1621 aattgtaccc taaacgtatt gctttaaata aatcagaaga agtcgcaaag ttcacttcag
     1681 ggctgatacg cttgattggt gtagtagcta agcccttcgt ctggctgctt tctgcttcca
     1741 caagtttatt ggcgcgtatc acgccgatga cttttgatga tgaagattca aaaatgacca
     1801 gagatgaaat gcgttatatg cttgaaaacg aaggcgtatt gaataatgaa gaattagaaa
     1861 tgctccaagg ggttttttct cttgatacga aagttgcccg agaagtcatg gtcccacgaa
     1921 cggatgcatt tatgattgat atcaacgaca ctgtggaaga aaatgtgaat gaagttttat
     1981 ccgaaaacta ttcaagaatc cctgtttata acgaagacaa agataaagta gtcggaatcc
     2041 ttcatacaaa gaatttacta aaagctgccc ataaattcgg ctttgataat ttagatatca
     2101 agaaaatcat gcaggagcct ttattcgttc ctgaaacggt ctttattgat gatctgttat
     2161 atgaaatgaa aaagacgcag aatcagatgg ctattttatt agatgaatat ggcggcgtcg
     2221 ttgggttagc aacactagaa gaccttttag aagaaatcgt cggagaaatc gatgatgaat
     2281 cagatgaagt tgagaatctt tatgaaaaaa tcgatgaaca cgaatatatc atccaaggac
     2341 gtatgctgat tgatgagttc aatgaagctt ttgacagtga tctgcatatg agtgatgtag
     2401 atacgatggc tggttatttg attacagcat taggcatgat tccggatgaa ggcgaaaagt
     2461 tatcgtttga tgtcgataat attacgctgg tttctgaaga aatggaaggt tctcgaatcc
     2521 ttaaaatccg tgtcattttt catgatccgg aagaaacaga agcggaacct gaagaagaac
     2581 gaagatactt cagaaaagaa ttcgaagatg acgagccaag aagataattg gaaacttggt
     2641 tatgaaagac gtgttgcttg aataaacaag caacacgtct tttcttttag tcagctgaca
     2701 aaaaaatatt tgactcttat ggtttggtgg ctttttcatt cgctgaatcc atgctatact
     2761 gattcagttg tagtagttga caaaggttgt gaaaaagcta tcctgcatta agtaataaga
     2821 gcagccaagc aaaaacagct tttcgtattt ttggcttggg tgggcttatt gctgataggc
     2881 tgtctctaga acgccgttta ctcggaaaaa gagactgcta cacggctttt gtcacagccc
     2941 tatatgaaat tgtctttctt taagagatcg gacaatctgt gcctagagaa aatataaata
     3001 attgaatgga agaaatggag ttaggttatg aataatccaa aattaaagca acaagtggac
     3061 aatcgcagaa cttttgcgat catttcccat ccggatgctg ggaaaacaac gattacagaa
     3121 caattactat tgtttggtgg ggctatccgt caagccggga ccgtaaaagg caaaaaaaca
     3181 ggtaattttg ccaaatcaga ctggatggaa atcgagaaac aacgtgggat ttctgttact
     3241 agttctgtta tgcagtttga ttatcaaggg aaacgagtaa atatcttgga tacaccagga
     3301 catgaagatt tttctgaaga tacttatcgt actttgatgg ccgtggatag tgcggtcatg
     3361 gtaatcgata gtgcaaaagg gatcgaagca cagacaaaga aattattcca agttgtaaaa
     3421 aaacgtggca tcccgatatt tacttttatc aacaaactag atcgtgatgg tcgtgaacca
     3481 ttggaattgt tagaagaatt agaagagcta ttagatatcg aatcttatcc gatgaattgg
     3541 ccaatcggta tgggtaaagg gctagaaggt ctttatgata tctaccataa tcgtgtagag
     3601 ttttatcgtc cggaaaatta tcaggatgaa cgattagtag aattggatga agatggactg
     3661 cttcccgaaa atcatcctct aactgaaaat tccttatatg aacaagtctt agaagaagta
     3721 gaactaatca aagaggctgg agatgcattt aatcaagaaa aaattgctcg tggcgatcag
     3781 acaccagtat tttttggttc tgcattgact aacttcggtg tacagacttt tttagagact
     3841 ttcgttgaat tggcaccagc tccttatgga cacaaaacac aagatgatca gatggtaagt
     3901 ccttatgagg aagaattttc aggtttcgta ttcaagatcc aagcaaatat gaatccagcc
     3961 catcgtgacc gaatcgcttt tgtccgaatc tgttctggaa cattcgaacg aggaatggat
     4021 gtctggttgg aacgtacaaa taaaaaactg aaattaagca atgtcacaca atttatggca
     4081 gattcgagag aaaacgtcga aaaagcagta gcaggcgata tcatcggtgt atatgataca
     4141 ggcaattatc agattgggga tacattgttc gaaggaaaac taaaagttgc ttatgaagaa
     4201 ctcccttcat ttactccaga gctgtttatg aaagtgactg caaaaaatgt catgaaacaa
     4261 aaatccttcc acaaaggaat ctatcaatta gttcaagaag gagcgattca gctttataaa
     4321 acatatctaa cggaagaata tatcattgga gctgttggtc agctacaatt cgaagtattc
     4381 caatatcgta tgttaaatga gtataacgcc gaagtaatca tgtctccaat gggacacaaa
     4441 atcgctcgct ggatcgatcc agaagatttg gatgagaaga tgagttcaag tcgcaatatc
     4501 ttggctcgtg accgtttcga ccaaccgttg ttcttattcg agaaccaatt tgcagaacgc
     4561 tggtttgcgg ataaatatcc caatgtaaaa ttaaaaagtt tgatgtaaag aagtcttgac
     4621 acaagctgcc ctgcatcaag taataagaat agccaaacaa aaacagcctc tcatattttg
     4681 tttagagccg tgacggattt tccgtgacgg ctctttttac tatattaaaa tcatagaaat
     4741 gtctaatcaa aaaaatataa aaattatttt gcgtacatat gattaaagaa aggagagtgt
     4801 gacacatgga aacagaatgg caaagacgtg aaagaaaaaa acagaaaaaa gaagagatgt
     4861 tacgattatg tcgattatta gatgtaaaag actgggacaa aaacgagcaa attgcacaaa
     4921 aagtacgatc aatcatccat aaagaggaaa aagttaaaaa taaagacacg cgtagaactg
     4981 aaaatgaaaa gagaaaagta caaaagaaaa gagaaaaatc aaaaagctag aaagaaaaag
     5041 aggctgagac agaagcgttt aactccaaga aataagctgg aattcacaaa aatttgaaaa
     5101 gcaattttcg tgaatttcag cttatttccg aaggagttgc ttctgctttc gccgtttata
     5161 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct ttaattatta
     5221 tgttcaaaat ttgtttacac ggctaatgta gcgcctaatt catgttttgg aatcgagatt
     5281 tcaacaaggc ctgccaattc gattactttg atttcatcct cgtggtaaac ggcgtctttg
     5341 attttatcta agaaataatt tttagcatct tcgatttctt tcggctggat tttacgtttg
     5401 tcattgatca gcagaatttc attatgttca ggggcttcag tgtagacaat agtggtattt
     5461 ccggctgtgt agaccttgac tagatgggca tcagtgtttt caagctggtt taatacaaga
     5521 cgggaatggc tgtttgttac atttaccaat ttcatggcgc tcacctcttt tatgtatttt
     5581 taattgcttg tttctcttat tataatttct tttttataaa atgaacagta ttttgacttt
     5641 tctgaaaaaa gaatgagacg gcatttctca aagaaatacc gtctcacgct ataaaactgg
     5701 atttattcag ccgtttcttc ggctgattct ttagccaaac gttctggttt agaagtaacg
     5761 atgatttttc catctttatc cagttttgct ttcaactcat ggatagaagg gtgatcaaga
     5821 taaaattcag cgataccatc ttcgatttgt tcttggatcg tgcgtcgaag aggacgagca
     5881 cccatagatg gatcatagcc taagtcaact aatttttctt tgacattcgt aggaacttcg
     5941 atgtgaagtt gctgtgctgc cagcatttgg ttgacatcgt ctagcattag tgtgacgatt
     6001 gtcatcaagt tttcttttga taatgcactg aattcgatga tcccgtcaaa acggttcaga
     6061 aattctggtg taaagtagtt gtttaattgg ttcaatacag aacgagtaac accttcgcgt
     6121 gccgcaccaa aaccaacgtt tgcttctact ttaccagttc cggcattact tgtcataatg
     6181 atgatcgtat ctttaaagct gaccgttctt ccttgtgcat ctgtcaatcg accatcatct
     6241 aagatttgca agaacatatg caaaacatct ggatgagctt tttctacttc atctaacaag
     6301 acaagactgt aaggatttct ccgtactttt tctgttaatt gaccagcttc ttcgtatccg
     6361 acataacctg gaggagaacc aatcaattta gagacactat gtttttccat atattcagac
     6421 atatcgaaac gaatcattga atcttgtgaa ccgaatagtt cgtaggcaag ttgtttcgca
     6481 agctctgttt tacctacacc agttggtcca acaaataaga aggagccgat tggacgattc
     6541 tgtttgttta atccaacacg gtttcggcga atcgctttcg ctactcgatc tacagcgtta
     6601 tcttgtccga tgacatgagc tttcaagtca tctgctaagt ttttcagttg tgtctgttct
     6661 ttttctttta attcacccac tggaatacct gtccgttgtt caacgatttt ttccatatct
     6721 ttttcggtaa tcacaggtgt ttcctcttca gtgagctgac gttctttcat tttttgaagt
     6781 ttattgatct gatcgcgata ataagctgct ttttcgaaat cttcttctcg agaagctaaa
     6841 actttttgtt cttctgcttc ttgcagtttt ttctcgattg ttttaggatc aacgatttgg
     6901 atcgtcaagt ttttcttaga acctgtttca tctagcaaat cgatggcttt atccggcaag
     6961 aagcgatctt ggacgtaacg attggataaa gttgcggctg cttcgatggc ctcatcagta
     7021 tattttacat gatgatagtc ttcataacgt ttttgcaaac ctttcaaaat actgatcgtt
     7081 tcttcgacag ttggttcatc tacccgtaca ggttgcatac gacgttctaa agcagcgtct
     7141 ttttcgatga tgcggtactc atttaaggta gtagcaccaa ccatttgcaa ttctcctcga
     7201 gctaaagctg gttttaaaat attaccagca tccatatttc catcaccagc agaaccagca
     7261 ccgacgattt cgtgaacttc atctatgaat aggatcacat tttctgcttg acggatctct
     7321 tcaatcaatt tttgcatacg ttcttcaaat tgtcctcgta taccagtacc ttggactaaa
     7381 gatacgacat ctaaacgaat gacttctttg tccattaatt tttgcggtac atcaccatct
     7441 acgatttttt gtgctaaacc ttcaacaact gctgttttac caacacctgg ttctccaatc
     7501 aagacaggat tgttcttggt tcggcgattt aaaatttcaa tgactcgttt gatttcgtca
     7561 tcgcgtccga tcacaggatc gatatcacct tgtcgtgctt gttctgtgat attgattccg
     7621 tattctccca ataaaccttc tgtattgcct tgaggtggct gaccaccatt gaagttgttg
     7681 tttccgccaa attgtgttgg tggtgtttgc tcataatttc cttgctgttg catttggcga
     7741 gacatggaac ggaaaagatc gtccaaactg ccaaatccaa atggatcatt ttgggccata
     7801 ttagtcagac ctccattttg ttgatttttg attttttgat aacagctctg acagtaatcg
     7861 agttgtgtcc gctgaccatt gacagtggca tacaagtgaa tcgttgcctc attcttgccg
     7921 cagttttgac aaagcatgaa tcattcacgt cctttcacaa aaatgggtac atccatattt
     7981 tacatctcgt ttttcatgag gtaaaagaat aacccctagt tttttaagag atttatgagg
     8041 tagaaaaggt gaaatctcta ttgaccattc ctgacctttg actcttatta tactaaaaat
     8101 aaaaagggaa gcaagtaatt cgtttcatat gaaaacaaag agggtcaaag gaaagaatga
     8161 taaacgtcag gagaagagaa agcgacaaga aaaaggaaat aaacacgttt gttataaaat
     8221 gtgaagaaaa ggatgaaaaa gaatccggaa taatttgaat acttttatct gaaattgaat
     8281 gaaaactgtt gtatctaacg aaaaaaaatg ttaatcttta cagatgaaag ggtggcattt
     8341 gttaggattc tctgaattac taaaaaagaa aaccctcaca taaaaacaac actcacttaa
     8401 aggagaccga ttaatatgga aaagaaagaa tttcacgtag tagcagaaac tgggatccac
     8461 gctcgtccag ctacactatt agtacaaaca gcaagcaaat ttaactctga tgtaaactta
     8521 gagtataaag gtaaatcagt aaacttgaaa tcaatcatgg gcgttatgtc tttaggtgta
     8581 ggtcaaggtt ctgatgttac tattactgca gaaggtgctg acgaagctga tgcaatggca
     8641 gcaatcgttg aaacaatgaa gaaagaaggc ttatctgaat aatggttgaa atgctaaaag
     8701 ggattgccgc tagtgacggt gtagccgttg caaaagctta cctgctagtt caaccggatt
     8761 taacattcag taaggcaaca gtagaagata ctgcggctga agaagctcgt ttggatgctg
     8821 cattagctaa atctactgaa gaattgcagc aaattcgtga aaaagcagcg caaagcttag
     8881 gtgaagcaga agcgcaagta tttgatgccc atttgatggt tctttcagat ccagaaatgg
     8941 taggtcaaat caagcaaaat attaaagaca acagcgtaaa tgctgaatca gcgctaaaag
     9001 aagtaacgga tatgtatatc ggtatgttcg aagcaatgga agataatgct tatatgcaag
     9061 agcgtgctgc agatattcgt gacgttgcta aacgtatttt agcgcactta ttaggcgtta
     9121 ctttaccaaa tccatcaatg atcaacgaag aagtggtcgt tgttgcacat gacttgactc
     9181 caagtgatac agcacaatta gatcgcaatt ttgtaaaagc gtttgtaaca gacattggtg
     9241 gacgtacttc acattctgct atcatggcac gttctttaga aattccagca atcgttggga
     9301 caaaagaaat tactgctaaa gtcaaagaag gcgatatttt ggctgtcaac gggatcgaag
     9361 gcgatgtaat catcgatcct acagatgaac aaaaagctga atttgaaaaa gcaggtgcag
     9421 attacgcagc acaaaaagca gaatgggaaa aattaaaaaa tgcagaaact gtcacagctg
     9481 acggcaagca ttttgaatta gctgcaaaca tcggaacacc aaaagatcta gttggtgtcc
     9541 ataataatgg tggagaagca gttggtctgt accgtactga attcctgtat atggattctc
     9601 cagacttccc aacagaagat gaccaatatg aagcttacaa agctgtatta gaaggtatgg
     9661 aaggaaaacc tgtagttgtt cgtacaatgg acatcggtgg agataaagaa ctaccatatc
     9721 tgcaattacc tcatgaaatg aatccattct taggatatcg tgcattgcgt atcagtttat
     9781 ctgaacaagg cgacgaaatg ttccgtacac aaatgcgtgc attgcttcgt gcatctgttc
     9841 atggtaattt acgtatcatg ttccctatgg tagctacact caaagaattc cgcgcagcaa
     9901 aagcgatctt tgaagaagaa aaacaaaaat tgataagtga aggcaaggaa gtatctgata
     9961 cgatccaagt cggcatcatg atcgagattc cagcagcagc agtcttagca gacaagtttg
    10021 caaaagaagt agatttcttc tctgtaggta cgaacgattt gattcaatac acaatggcag
    10081 cagaccgtat gaacgaacgc gtttcttact tgtaccaacc atataaccca tcaattttac
    10141 gtttgatcaa aaatgtaatt gatgcagcac acgctgaagg aaaatgggca ggtatgtgtg
    10201 gagaaatggc tggagatcaa acagctgttc cattgctagt aggtatgggt ctagatgagt
    10261 tctcaatgag tgcgacatct attcttaaaa cgcgtagttt aatgaaacgc ttggacacag
    10321 cgaaaatggc tgaacttgct gatcgtgcat tgaaagaatg cgatatgatg gaagaagtcg
    10381 ttgaacttgt tcatgaatat gtaaaataaa caacgtatca aaagctggtg tggcttgtaa
    10441 aagagctacg ccagtttttt tgttcttttt taagaaaacg tttttgtcag taagaataga
    10501 caaaaatagg gcttaagttt gagtgacagt ttgtaggcta tgttatacta tataagtgaa
    10561 gggggacgta aggtgaaggt attattatat tttgaaagtg aaaagatgct tgcaaaatct
    10621 ggaatcgggc gtgcgcttga tcatcaaaaa cgtgctctga cggaagtcgg tatctcttat
    10681 acactagatg aaaaagaaga ttatgacatc ctgcacatca atacatacgg aatcaatagt
    10741 cataatatgg tcaataaagc cagaagagaa ggaaaaaaag ttgtctatca tgcgcattct
    10801 acagaggaag atttccgaaa ttcctttatc ggatcgaacc agctttctcc tatcgtaaaa
    10861 aaatatctgg taggattgta ccaaaaagcg gactatctga tcacgccaac accttattca
    10921 aaacagttgt tggaaagcta tggtatccgt gtgccgattc aagctatttc caatgggatc
    10981 gacttagaga aatatcaccc tgatccaatg aaagaacaaa agttcagaga atatttcaaa
    11041 ctgtctccgg atcaaaaagt aatcatttgt gtcggtttgt ttttcgaacg taaaggaatc
    11101 attgatttcg tagaaattgc taagaagatg cccgaataca catttatttg gtttggtcat
    11161 gttcccatgt actcgattcc tcggaacatc cggaaaattg taaaagaaga tcatcctgaa
    11221 aatgtcctgt ttcctggtta cattcgtgga gagatcatag aaggtgcgta ttcaggtgca
    11281 gatctattct ttttcccttc ttatgaagag acagaaggga tcgttgtttt agaagcttta
    11341 gcaagcaaac aaaacgtact tgttcgagat attccagtgt ataatggctg gttagaagat
    11401 agcaagaact gctatatggg gaagacaaac gaagaattta tccgactgat ccaacagatt
    11461 accaatcaag aactaccgag tacgacacaa gccggttatc aaacagcaaa agagcgaagt
    11521 atcaataaaa tcggtgaaga acttaaaaat gtatacgaaa gtgtactgaa tgaaaaagtc
    11581 tctagcaaat taaaaaaagt aaatcaggtc aaggactagt ttggaggcga aaaacatgaa
    11641 aatcggcttt tttaccgata cctactttcc gcaagtcagc ggagttgcta catcgatcaa
    11701 aacattaaaa caagaattag aaagacatgg gcatgaagtc tatattttta caacgaccga
    11761 tcctaatgcg gataagttgg aaaaagatgt gattcgaatg ccaagtgtac catttatttc
    11821 tttcaaggat cgaagagttg ttgttagagg catgtggtat gcttatttgg ttgccaaaga
    11881 actagaacta gatcttattc atacccatac agaattcgga gcagggatac ttgggaagat
    11941 ggtagctaag aagttgaaaa tacctctgat ccatacttac catacgatgt atgaagacta
    12001 ccttcattat atcggtaaag gaaaagtgat caggcaatcc catgtaaaat acttgtctag
    12061 attatttgca aatcatacga caggtgtagt ttgtccaagt gaacgtgtta ttgatacatt
    12121 acgcagctat ggtgtaatag caccgcttcg cgtgattcct actggtatcg acattgaaaa
    12181 attcaaacgt tctgatatca ctcagcagga tatcagcaaa ttaagggagt cactaggtct
    12241 tcaggaaaac catttgatga ttctttcttt aagcaggatt tcttatgaga aaaatatcca
    12301 agccttgatt aatgggtttc ctcaggtcat ctccgcttat cctaacgcca gattagttat
    12361 cgtaggaaaa ggaccttatg tagaagagtt agaggaattg atctcagaac tgcagttaga
    12421 agaatttgtg cagttcacag gagaagtgga caacaatgat gtagcacttt attataaagc
    12481 ggcagattat tttgtcagtg cttctacttc agaaacacaa gggcttactt atacagaagc
    12541 gatggcagcc ggaacacctt gtgtcgtgga aggaaatgct tatttgaacc gattgttcga
    12601 tcatccttca ttagggaaaa cttttgaaac cgacgaagac tttgcaccag cacttattga
    12661 ttatatttcc agtgaagttc ctaaagatcc aaaagtttta gaagctaaac tttatgatat
    12721 ttcatctaca catttcggcg aagctatgat cgaattttat caagacatgg ttgtttatca
    12781 tgaagaagag atgcgaagaa aagaagaaga agactcgatt gaacgaatca aaataaaatt
    12841 aaattcattt aaaagataaa attcaaacat tttttatgaa aaaatccttg tttctagcgt
    12901 atactagcat agaagcaagg atttttgatt tttaggaggt cgaaaaaatg aaacttggtt
    12961 ttatcggaac aggcgtcatg ggaagtgctg tcgcacgcca tctactagaa gcaggacacg
    13021 aagtagctgt ctataatcgg acaaaagcaa aagctgatcc tttagtaaca gaaggagcta
    13081 tctgggcaga tacaccaaaa gcggtagcag aacaaagtaa tattctcttt acaatggtag
    13141 gttaccccaa agatgtagaa gaaatctact atggacagtc ggggattttt tctgcagata
    13201 tctcaggaca tattttagtt gatttgacta caagtacacc aagcctggca gaaaaaattg
    13261 caaaaacagc aaaagaaaaa ggcgcagatg cgttagatgc accggtatcc ggtggtgact
    13321 taggagcaaa gaatggtaca ctgacgatca tggtcggtgg agaagaagcc gtttatgatc
    13381 aagtactgcc tttattcaaa gaatttggta cgacatttac attgcatggt tctgctggga
    13441 aaggtcagca tacaaaaatg gctaatcaga tcatgattgc aggtacgatg acaggaatga
    13501 cggaaatgct tgtctatgca caaaaaaacg gacttgattt aaagaaagtg attgagacgc
    13561 tttcaggtgg aagtgcagct aactggtctt taagcaatta ttctcctcgt attttaaaag
    13621 aagattacac acctggtttt tttgtcaaac attttatcaa agatttaaag attgcattag
    13681 aagaagcaga aaaaatggat cttgtattgc cagcaactac ccaagcattg aaactatatg
    13741 aagagttggc agataaaggt tttgagaatg acggaaccca agctttgatc aaactttggt
    13801 ggcctgaagg aaaaatacca gaaaaaaaat cataaaaaac gagttttttt agagaaaatc
    13861 aatgcaagta ggctatgctt ttgatacaat ataacagaaa taagaaaagg aggacctccg
    13921 atgacacatt cacaattagt cgcaatcata aaacgactgg aggctatggt cgagtcagca
    13981 gataatgaac tgcaagttcg ccgttttgaa aaagaaggcg tagagaagtg tacagtaact
    14041 tatgataaag cgaccgatac atttgaatta actgaaacag atactcatca gcaatacgaa
    14101 tttgataata tcgatatcgt agcgatggaa atctatgact taatccaata aattttagga
    14161 taattgacat ttgaagttga agatgcctaa aagaagccga gaagcaacta atttagttgc
    14221 ttttcagctt cttttttgtt taacagttca atttcaaact gttctttttc taattttttg
    14281 cggtaactga tcaagatttg tacaggatcg atttcttctt ttgacaattg acgtttcaaa
    14341 gataaatgag cttctttctt ttcataatag aagtcaggag aaacgtcacc agaggtaatc
    14401 attgtttctg caagttgacc ataacaaacg aggtagtgct ttttctgact ctcatctgct
    14461 gctaatggca tgctgaaggt gagcgatggt gttcttaaaa actgcttttg ttcctggatc
    14521 cgtctaataa aataagtaac taattcgata tctttttgaa gcataccggc tttttcgaaa
    14581 ttcaactgat agatgaaatg ttgttgtctt tttttcaagt aagtcagtag ctttttattt
    14641 cggccttgaa acagcccttg gagttccttt tttttctgtt caaaagtcat ttcctgtagg
    14701 tcaggaagct ggactcgcaa agctaacaaa ctaatttcag acaaccaagg catttgataa
    14761 gtttcttcca ttatacgcaa aatagacggc atttttttat agagacgaaa aggcccgtat
    14821 gttcgagccg taggagtatc tgttaataca aatcccttat tggtaatatg tacataatta
    14881 taattactga aataattcat ttgtcggtta taaaaaggac ggtaatgttg gatcaattga
    14941 cactcgagca ataaagcatc taattccgtg tctactacga cgaaatcaaa atctgttata
    15001 tgatgaatca tacgtaatac tttttttgaa tgctgtttat tctgatgaaa atagctgctt
    15061 actctatttt tgagccgttt tgctttacca acatagatga ccttaccatc tcggtctttc
    15121 ataagataga cacccggcag catggggaga gttttcattt tttcttttaa cataggatct
    15181 ccttttacgc tttgaataaa aagactataa caaattttga aataatggac aagttttagg
    15241 gaattctata gttctgttca cagaaaatac tttgataatc aaaatatgtg a
//
LOCUS       DAZKCY010000062        15288 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000062 DAZKCY010000000
VERSION     DAZKCY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.062"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..2101
                     /locus_tag="ACG8XC_001737"
     CDS             41..2101
                     /locus_tag="ACG8XC_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFP9191432.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2184..2753
                     /locus_tag="ACG8XC_001738"
     CDS             2184..2753
                     /locus_tag="ACG8XC_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9191433.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2928..3734)
                     /locus_tag="ACG8XC_001739"
     CDS             complement(2928..3734)
                     /locus_tag="ACG8XC_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191434.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3872..5386
                     /locus_tag="ACG8XC_001740"
     CDS             3872..5386
                     /locus_tag="ACG8XC_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFP9191435.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5789..6772)
                     /locus_tag="ACG8XC_001741"
     CDS             complement(5789..6772)
                     /locus_tag="ACG8XC_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFP9191436.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6777..7424)
                     /locus_tag="ACG8XC_001742"
     CDS             complement(6777..7424)
                     /locus_tag="ACG8XC_001742"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9191437.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7509..8141)
                     /locus_tag="ACG8XC_001743"
     CDS             complement(7509..8141)
                     /locus_tag="ACG8XC_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9191438.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8157..8708)
                     /gene="coaC"
                     /locus_tag="ACG8XC_001744"
     CDS             complement(8157..8708)
                     /gene="coaC"
                     /locus_tag="ACG8XC_001744"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFP9191439.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8705..9442)
                     /locus_tag="ACG8XC_001745"
     CDS             complement(8705..9442)
                     /locus_tag="ACG8XC_001745"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFP9191440.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9439..10428)
                     /locus_tag="ACG8XC_001746"
     CDS             complement(9439..10428)
                     /locus_tag="ACG8XC_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9191441.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10686..11228
                     /locus_tag="ACG8XC_001747"
     CDS             10686..11228
                     /locus_tag="ACG8XC_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9191442.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11245..12330
                     /locus_tag="ACG8XC_001748"
     CDS             11245..12330
                     /locus_tag="ACG8XC_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9191443.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12342..13148
                     /locus_tag="ACG8XC_001749"
     CDS             12342..13148
                     /locus_tag="ACG8XC_001749"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9191444.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13149..13976
                     /locus_tag="ACG8XC_001750"
     CDS             13149..13976
                     /locus_tag="ACG8XC_001750"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9191445.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            13973..15046
                     /locus_tag="ACG8XC_001751"
     CDS             13973..15046
                     /locus_tag="ACG8XC_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9191446.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4904 a         2765 c         3130 g         4489 t
ORIGIN      
        1 tacttcatgt ttagacaaga gtagggagga ctaagaataa atgtgttcaa aaagacgttc
       61 aaaacaacta aataaacacg cttgtcgttt agataaggaa cggtcgtgtt ctgaaaaaca
      121 ctacgttaag tttatgatgc gcacgatgct gatcagttta tctgtaggtg tctatatgca
      181 tgtacccgtt caggcaacga cacaaaccat caaggtcgta cgtacctctg tttctgagac
      241 cactgattcc cctatcacta ttactcatac tttcactgat gcaaatggaa aacagctact
      301 tcctgcattg acttcctata ataaagtcag ttatcctcca acgataaacc actactggtt
      361 aattcctaac aaaacattat ggacatcgga aaccggaagc agtgtgccgc tttctatctt
      421 ttccgatatg actgatccta caaaaggtga tcagttaaaa cagatcactg atattttgaa
      481 tcatgctttg acagatataa aggacggtgg aagcgcacaa gtttcttatg tatatgaaaa
      541 agattattca cagtttgaaa gaaagaaaat cgatgttctg gcagggacaa aagtttcgat
      601 atctgatctg gtagaaaggc tgaagaacgc agatggaaca caaggagata tgtccaaagt
      661 aatttcaaac aatgggaata tcgtggatac gactaataca ggaacatcta ctatttcctt
      721 gtcctatttt gatccaattg ctttaaaaac aatgactgca tctgctgtga tccatgttcg
      781 tgctgatcaa acagctatca aaggaaaaga tcttcactta agaacaggag agagttacgc
      841 ccctaaagat cttgttgaat atgtaacaaa aagcgatgga acttcaggaa ataaagaaga
      901 aatcaaaatg aacgggacag tagatactac aactccggga gaatatcccg ttgtattaac
      961 ttatatagac tcgaaaacat caaatgtggc agaaacaaca gctgtagttt atgtagaagc
     1021 atctgctgct tcatcgactg tcatcactca ttttatagat agtcaaacaa ataaagaact
     1081 tcaagcagaa acacatatga gtagtgtaaa tcgtattcct gatttgcgcc tacctgctga
     1141 attttctgat cgagtgccaa atatagattt atcgacttat gaacaaaatg ggaaagtcac
     1201 ttctatttct gattggatgc aagcaaacgg attagacaga aaaagtcact ggtcggagat
     1261 ccttgaagta cttcactcag aactgatgaa taataggaag caatcaggag gggaaattac
     1321 ctttacttat gtttatatac cagacaatcg ttcattagaa ggtcatgatc ttattgttca
     1381 tccaaacgaa aaaataaaca aagaagagtt gatccaatca gcaacaaatt ccttaggtaa
     1441 acccgtaccg ttaaaggatg tatcggttac aatcaatgaa gtaccattgg aggatggtta
     1501 tttgaccaaa caaacaggtg attttacgat tatgtatagt tatcatgacc cgtattcact
     1561 ccaagaactc catgctcagt cactattaca tgttttacca ttaaacaaac agatagatca
     1621 aaaagaacaa aaaagacaag aagaaagctt gtcagaaaaa aaacaacaaa tggctcatgc
     1681 agtttctcca acagggaaag taaaacaaac aagaagccta gagttagcaa gaatatctag
     1741 aaaaatctca gaatctaata aaacgataag gcaagaagct catttggccc cacaagtttc
     1801 tggattggaa aatgaaattt tagagcctaa aataaaacaa caatcagaaa aagaatttgt
     1861 tgctaaacag tcggaagatc agaaggaatc agatgaccag aaggaaacga agaaaaaacg
     1921 cttgtcaact attgattcca ccgatgagca tctgcagccg caagtaccac ttcctagtcc
     1981 gggaacaccc gcacctggtg gaggtattcc ttcttctgct ttaggagatt tcatgcgagg
     2041 tatggcagga acatgggtat acgctaatcg aaatgaattt gacgcagatg actggttctg
     2101 atttatatct ctatctgtta tagtcaaata agtagctgag tcatatgtta tttggcataa
     2161 tggagacgag gagggataga aaaatgttag ataaaacgtt accttacgca gaaatctgga
     2221 tgacaagacc acttcaacaa aatgttccaa tcacgccttt agcggaaggt tttgaatttc
     2281 gattttatca aaaagaagat gaatctgctt gggcggagat tgagacttcc gttggggaat
     2341 ttcaaacgac agatgaagca cttgagtatt ttcaacaaaa ctttgctcct tatcctgaag
     2401 aacttgaaag aaggatgttt tttgtagaga caaaaaaagg agaaaaagta gcaacctgta
     2461 ccgcttggcg aaaaaaatca agagagcaag tgtatccggt ttttcattgg ttagcagtga
     2521 aaccagatta ccaaggaaaa gggctcgcaa aagcattgac tgccagagtc ttgcaagaat
     2581 ttcctaccct ccatacggac ggaccaatct atcttcacac acagacatgg agccatcagg
     2641 cgattgctct ttataaaaaa atgggtttta cttttattgc tgaaaatcta gacggcacac
     2701 ctaatccaga ttatgaaaaa gttatgcagg ttttaagtaa attagataaa taacgaaatg
     2761 gtgaaataag tcctcagtat tgggataaaa aacgaaatag tcattcctca tatcttactt
     2821 ttttgagtta atactatcgg ataactaatg gcatgccata cattcaaaaa agaaggggca
     2881 gtgacaaaag tattgtcact gccccttctc ttgtttttta gcatgtttta tcgtttgatt
     2941 tttttaggaa taaaattctt gatgaacgta aatttttggt aatcgtttcg actatagagg
     3001 taaccaaata cactgaaaac aactgctcca ataaaattaa caaataaatc cttcatagta
     3061 tccatgattc ccacgtctaa gtaaccattt tggatgattg ttttatctgt tttaccatct
     3121 gcattttgac tttcaatgat tgtccgagtg atatctggaa catgatagac agtgtttccg
     3181 tctttactta actccacact attgatttgc tggatgatcg tatctttttg catatcgaat
     3241 tgcagtaatt tatctgctga aaattccaca aactcccaca taacaccaac tgtcatcgaa
     3301 aaacagaagg aaacaactgc tagtaataaa ggagtcaagt tcatggtttt tgtattttta
     3361 ttgagcaaat aaacaaggga aaagccgaca cccccagcta aaaatccgct gaatgcatgt
     3421 agaaaagtat cccacattga aaaatagcca taaaaatcac ttaactctcc taaaactgtt
     3481 gaactaaaaa taaaagataa caaaatgatt tctagcatat ttggaatctg tatctttaat
     3541 atcttttcca gaaaataagg gatttcaaat aaaattaatg tcagtaataa aagcattgta
     3601 tttccccagt gttggttcca gacctgatcc actagcataa taacgacgat gatacgtaat
     3661 aaaatataga ttccgtttgt gattctacct gcagtttttg tactgttcgt aaaaaaggtt
     3721 ttcaaatgat tcataaaata actccttcat gtatatcctt aattatacta agaagcgtgt
     3781 gtttaccaaa gctatatgac ttaaagaaaa acagagttga tattgtgaaa aaaatcactt
     3841 aatatagtat aaggagaaaa gggggataca aatgaaaaaa ttagttgctg gtttattaac
     3901 agccgttgca cttcttgcta tgagcgcatg cggcgcagat cagaaaaata cagcgcagac
     3961 aagttctagt acgaaggaat ctgccgaagt atcatcaggc gcttctgaac aagtatatac
     4021 agatccaaaa gaaatgaaag aatcttatga tgtcgtgatt attggagctg gtggtgcagg
     4081 gatggcagca gctatcgaag caaaagatgc tgggttgaat ccagtaattt tggaaaagat
     4141 gcctgttgcc ggaggaaaca catctaaatc atcaagcggt atgaatgctt cagaaacaaa
     4201 atttcaaaaa gaagaaggaa tcaaagactc gaatgatgcc ttctatgaag aaacgctaaa
     4261 aggtggtgga ggaacgaatg ataaagaaat gcttcgatac tttgtcgatc actctgctga
     4321 tgcggtcgat tggctagatc aaaacggtat caaactggac aacttaacga tcactggagg
     4381 gatgagtgaa aagaggacac atcgtccgtc tgatggctcg gctattggtg gatacttagt
     4441 ggaggggtta ttgaataacg tccatgagcg ggaaatccct atttttgtca acgcagatgt
     4501 gacagacatt aagaaaaatg gggaaacggt caatgaagtg actgtacatg ttcaaggaga
     4561 agaaccaaaa gaggtcaccg gaaaagcaat cgtagttacc acaggcggtt ttggtgccag
     4621 caaagaattt attgaaaaat atcgtcccga tttagctgat tatattacga caaatcaaga
     4681 aggatcaact ggagatggta tcaccatgat tgaaaaatta ggtggacaaa ccgttgatat
     4741 ggataaaata caaattcatc caactgtgca gcaggatgag ggggtcttga ttggtgaggt
     4801 tgttcgcgga gaaggagcaa tcttggtcga tcaagagggg aaaagatttg tcaatgaaat
     4861 ggatacaagg gataaagtat ctgcagcaat tacagcattg cctgaaaaat cagcttattt
     4921 gatttttgac caaggcgtgc gtgatcgtgc cacagctatt gaattttatg accaaaaagg
     4981 atatgtcaca actggaaaga caatggaaga attagcagaa aaaatcgatt taccaaaaga
     5041 aacactagca aaaacagttg atggatggaa tacggatgtt gcggagaaaa aagatgctca
     5101 attcaaccgt gccacagcca tggaacatga cttatcgtct ccgaattatt atgcgatcaa
     5161 aatcgcacca ggaatccatt acacgatggg cggtgtgaaa atcaatacga acactgaagt
     5221 attagataaa gaaaaccagc caattagggg gttatatgct gctggtgaag tcacaggtgg
     5281 cttacatgga aacaaccgga tcggtggaaa ttcagttgcg gagatcatta tttttgggcg
     5341 acaagcgggc atacaagcag ctcgatttgc ttcggaaaat gaatgaaata gaacgtaaat
     5401 aaaaagaggt tgtgaaatca gcgttttgag ctaataccga gggtcagctg attgaacact
     5461 gtttagtgaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa
     5521 aaacagcttc ttatgttttt aacttggttg gacttattac cgcagatgca agcttttgaa
     5581 caccgtttat tcggaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc
     5641 taaacacgta taaacggcgg aagcagaaga aattccttcg gaaataagct gaaattcaca
     5701 aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc ttggagttaa acgcttctgt
     5761 cccagcctct tttttgatca gcatgatttt attcagttat cttcaaaatg atttttccga
     5821 tgtttttgtt tgcttccata tattcatgcg cttgtttgac tgcgctgaat tggaatgtac
     5881 ggtcaatgat tggccgaatc ttgccatttt ctagaaaagg cccagctttt tgcataaaag
     5941 tttgtgcgag ctctgcttta tactgatcac ttctaggtgt caataatgta ccaagcagtt
     6001 ggacacgttt cccaatcaat acaccaaggt ctatctcagg cacgatggtc ccacctagca
     6061 taccaatcaa gacccaacgt ccatcaacgg ctatagaagc taaattcttt tcccagtaag
     6121 aagcgccgat gaaatccaaa ataacatcga ctccccgacc ttcagtgatt tttcgtacct
     6181 cttcttgaaa gtcttgtttt ttatagttga tcaattcatc tgcaccaagt tctttacaaa
     6241 aggcgagttt ttcttctgat cctgcagtaa cgatgatttt cgcgtgagaa agctgtctag
     6301 ctaattggat cgctgctgtt cccacaccac ttgctcctgc atggatcaag acagtttcgc
     6361 cttcttgtaa ttttccaagc cagtaaagtg tttgataagc agtcaaaaaa acttctgaga
     6421 ttccagcagc agaaacatag tctagcgtat cagggagaag gattgcacga tcggcaggca
     6481 tcacagcata ttcggcataa ccacctagat tgactaatcc gtatactttt gtcccaggag
     6541 taaaagtgga gatatcactt ttattctcaa ctacctctcc agctacttcg actcccaaaa
     6601 tagggtaggg aggtttcaaa gagactgatt ctcttttcat aatatctgtt cgattgacag
     6661 ctgctgtatg gactttgatc agtaattcgc cttgttttgg tttaggaaca ggcacttcaa
     6721 tttgttctaa tacatctaca gaaccaggat gcttgattga aatggcacgc atggatctat
     6781 tcctcctttg cccattcttg gaatgctgta ttcaactctt tgctaccttc agaaagtact
     6841 agagtcgtac gagaaatatc cgtttcttcc actagagcgg caagtacagc agcaactgta
     6901 gagcgagtga tcgtttttga tgccttctca ttaggcttag ttaattgaat ggaagtaact
     6961 tcttcatcgt tagtcaatgt gactggttga agaatgacaa aatctaagtt agttcgtttc
     7021 agccattcat ctgcataatg tttagtaata taataatctg tcatggactc aggccatttt
     7081 gctggttcat ctgcataaac agcactgacc atcagatagc gagagacatg cgcattttca
     7141 gctgcgatca ctgttttgat tgcgccatcc aagtctactt gcagaagatt ctttccttga
     7201 gagccggcag caaaaatcaa tacatcgatg cctttgaatg cttcggtcat tttttccacg
     7261 gaccaagtta aatcaaaaga gacatacgaa acattctctt tttttactaa tgtttgggaa
     7321 gcaacatctc tcacccctgc aaaaatttca tgggaacttg gagctaaatc ttttatcagg
     7381 tgtcggccaa tttgtccgtt tgcacctaca acaaaaattt tcatttggat catcctttcg
     7441 acgtcaaatc tttccattca atagtaacac caaaagagaa aaaaatcaga gatcggctac
     7501 gatgactatc agttagtaag agaagggtgc ttgaagtatc ttaacaagac attgcatact
     7561 gctgcagtcg caagacctgc gacgattcct tcaggtatac cacttgtaaa aataaccgct
     7621 agaatagctg aataaacagc atttaaattt gttccgatga ctttggcata atcttgctgg
     7681 aataaaaagt agatcatatt catcactaaa atcgtattgg tcaatgaacc ggaaagtcca
     7741 gctaagaata aagaaacgga atctgctttt tgtttcatta gtttgcgcag acctttataa
     7801 acgaaataag gaaccacccc aatcaagatg cgaggaataa acgcgatcag caaggctttc
     7861 cagcttccat gttctgttcc gatgactggg ataaacggag aaaagacaaa agacaaaggt
     7921 gtttggataa ctgtgctacg gatcatgctg atcagtccaa atgtaccgcc aagaaaagca
     7981 cctagtcttg gtcctaaaac gatcgaagca atgattacag gaatatgcat ggttgttgca
     8041 ttgattggac cgatagggat aaaacctaaa ggcgtaacag ctaataaaat caaaatagct
     8101 aaaaacatcg cagttaatgt aaagtttttt gtatttttca taagtactcc ttttttctaa
     8161 gcatttcttt cattaagagt cagcatgacc tgttcgatga tcgtctgaat atcagctaag
     8221 gcaccttttc caaaatctcc acaagctaaa agagattctc ttggttcaat ttcctgatag
     8281 ccgacagatc tcaatgtttc cagattacgc tgagtgatcg ggttttgata catattcgtg
     8341 ttcatggcag gggcaatcaa ttttggtgtt cccactggaa gcgccattgc gactgttgaa
     8401 agaagatcgt ctgcaatgcc gtttgctagc ttgccgataa tgtttgctgt agcaggcgcg
     8461 atgagaaaca agtcagcttg ttttgctagt tcgatatgat tgattacctg cggatttgat
     8521 tcttgcatga cgtctgtatg gactgcgcgc ttcgacagag attgcaaggt taatggcgtg
     8581 ataaaatgtg tgctgctttt agacataatc acatctacct gggcacctaa tttgacgaat
     8641 tgacttgtaa gatcagcgct tttgtaagca gaaatacttc cagtaacgcc tagtaagatc
     8701 tttttcattt tgattcctcc agtctaattt tctcagtaat caatgcagca atctcagatt
     8761 tactttgagc ttcttccact gttccatctt tagataagag atagccgtgg tgtgtctcgc
     8821 catggatttc cgtcaaatca tttgcgaaaa taaaatcagt tttgttattt atcagactat
     8881 tttttgcgac tgccagaaga gattcctttg gtacatctac taatagtttg aaaccaatga
     8941 tttttgcttt tggctgccat tcacgtagat aagaaatgat ttttggtgtt ttcttcaaaa
     9001 cgagcacaag atgctcggtg ttagaagaga tctttgaaac agtggtatct gtctgttcat
     9061 tttccgcaaa ccaattgtcc agttcttctt gcgttgaaga agtgggtagg ttttttgcca
     9121 gctgttcttc tgaaaaacta aaagcaggag taaaatcact gactgccata ctgtggataa
     9181 tcgcatcgta gtgttctttt ttcgttagtt cttctaattg taaaaataag tccagtgcag
     9241 attcgatcgt ataaatcgtt atattggagt gtcttttcgg tttcagtgct tgtttcgtag
     9301 tcacgtaatc gattgtggta tccggattgg cagctaaata atcagctaat gcttgcccta
     9361 atcgtcctgt cgaatgattt gtaatagcac gtacttgatc gattttttca gtcgttcctc
     9421 cagctgtgat tagaatcttc atttatggtc acctacattt actagtgttc ttccttggtg
     9481 tgttccagca agtagtttag atgctgtttc aggcaattga tccagagtaa tctcttggac
     9541 gaccagttca tctgcaatct gcaaatccgc catctgttgc cataaagaaa gacgttgatc
     9601 tattggtaca ttgacagaat caattccaat caactggatg tttcgcagaa taaaaggcaa
     9661 gacagtcgcg tttagtttga ttcccccagc attgccacat aaagctactg caccgttata
     9721 agagatcaat ggaagaagac tgcttagctg ctcgccacct actgtgtcga tgacatagtc
     9781 gatttgctgt tttcctaaag cttttgttgt ctctggcaga aattcatcag gtcgtacgac
     9841 ttgcaaagca cctagacttt ttagccatgt gacagactct tcttttctgg aaaaggcaat
     9901 gattgactgg aatcctagtt tatgtaacag ggctatcgct gtactgccta caccgccaga
     9961 agcaccagta acgacaactt ttttatcgga ggtcatacct tgacggatta aagcgttcac
    10021 acacaacata gcagtaaacc ctgctgtacc aaggatcatc gcttgacgca gcgtcatatt
    10081 ttttggtaga ggaactaacc agtctcctgg tactttttga tattggctat aaccgcctgg
    10141 atgggtgacg ccaagcccaa aaccagtagc aagtacaaga tcttcttttg aaaaacgtgg
    10201 attgttactt tggacgacag ttccggataa atcaatccct ggagtcatcg gatatgttcg
    10261 tatcacctga ccagactcgc tcattgccaa tgcatcttta taattgacat cagaataggc
    10321 aatttttacc aagacttcat tttcagccaa ctcagtaaat ggcagatcac tgattgctgt
    10381 atggaaaaca ggttccttta ttacctgaaa agatcgaaaa gcgttcattc gtgagacact
    10441 ccttttaaaa ttttctttta tcataccata aagcctactc ttttattctt gttcttccgt
    10501 aaatttagac aaaaattagg tgaaaaataa agaatcaaac aactagaatt tattccaaaa
    10561 gataaagaaa tattcagcaa aaaaaatttt acaaagggtt gcctttttca ataaatttcg
    10621 tatactaata cgagtggtag taaacaggaa gtttactatt agaaaacttc gattcggagg
    10681 aaattatgga aattggtgag aagttaagaa atttgcgcat ccaaaagaat ttgacacaag
    10741 aagaactagg cgaacgcaca gatttgtcaa aagggtatat ttcccagttg gaacgagatt
    10801 taagttcacc atcaatggaa acattctttt ctattcttga agtcttaggt gtgactccag
    10861 aagaattttt tcatcaagaa gcagcgaatc tgcaagtggt ttattcaaaa gaagatcata
    10921 cggtttattt cgatgaagaa aacgggtatg aattggaatg gctcgtggct gattcgaatg
    10981 aaaaagaaat ggagcctgtc ttgctgactt ttgaaggtgc aggagaatat aagacgtttg
    11041 aaccttcatt atctgaaacg tttatttttg tattagaagg ggaattagag ttaagcttag
    11101 gagagacggt ctatgtggca aaagcaggac agtcgatcta ttatcaagcg acaaaacaac
    11161 atcaattaag gaatcatctc aaaaaacgaa caaaagtatt gattgttgca acgcaatcat
    11221 atttgtaata tagggggtaa aaacgtgggg aaaccaatca tttcatttga tcaagtagtg
    11281 aaacgctatg atgatgaaac agtattaaaa aaagtaagtt ttgagatcga acaaggaaaa
    11341 ttttatacac tgcttggtcc ttccggctgt ggtaaaacaa cgatcctacg tattatagct
    11401 ggattcaccg aagctacaga aggggacatc tattttgaag ggaaacggtt gaatgatttg
    11461 ccagctaata agcgacaagt aaatacagtc ttccaagatt atgcactttt tcctcatatg
    11521 aatgtttttg ataacgttgc ttttggattg aagatcaaga aaatgtccaa atcagatatc
    11581 gaaaagaagg taaaagatgc tttgcgtatg gtccagcttc ctggatacga aaaacgggag
    11641 attagtgaaa tgtctggtgg tcaaagacag cgtgtagcga ttgcacgagc aattgtaaat
    11701 gaaccgaaag tattgctatt agatgaaccg ttatcggcgc ttgatttgaa actacgtaca
    11761 gatatgcagt atgaactgcg ggatctgcaa caacgattag gaatcacgtt tatttttgtt
    11821 actcatgacc aagaagaagc gttggcaatg agtgatgaga tttttgtcat gaataaaggg
    11881 aaaatcgtcc aaagtggtac acccgtagat atttatgatg agccaatcaa tcatttcgtt
    11941 gctgattttg ttggggagag caatatcgtg gacggtgtaa tgatcgaaga taatctagtt
    12001 gagtttgttg gtaagcaatt tgaatgtgtc gatggcggga tgcgtctaaa tgaaccagtt
    12061 gaagttgttt tacgcccaga ggatttgacg atcacgacac cagataaagg aaaactagtc
    12121 gtaacggtcg atactcagct gtttcgtggt gtccattatg aaatcatctg ttatgatgaa
    12181 cagcaaaatg aatggatggt ccattcaacg aaaaaagcta aagaaggaag caaagtagga
    12241 ttggcctttg agccagaaga tatccatgtc atgcgtttca acgagtctga agaagaattc
    12301 gatgcacgtc ttgacagcta cgaagaatag gggggacaag aatgaaaaca atgcgcagga
    12361 tctattccgt cccttatatt ttgtggctcg gtttattcgt gatcgcgcct gtattaatga
    12421 tcatttatca atcttttttt gatatgaatg ggcaattcac gctgaataat tatctatctt
    12481 attttacgtc ggggacatcg ctttctatga cgatcaattc tgtatggtat gcatttttga
    12541 tcacgttttt cacgctgatc atcagttacc cgactgctta ttttttaaca aaactgaagc
    12601 acaagcaatt atggctgatg ctgattattt tgcctacatg ggtgaatctg ctgcttaaag
    12661 cttacgcatt tattgggatt ttcagtgtgc acggcagcgt gaatcatttc tttgatttta
    12721 tcgggatcgg gccgaaacag atattattca cagacttcag ttttctgttc gtggcaacgt
    12781 atatcgagat tccatttatg attatgccaa ttttcaatgc attggaagag atgaatcctt
    12841 ctttgatcag tgctagtaga gatttaggcg cgaatagctt tgagaccttc cgacgagtga
    12901 tttttccgtt atcattaaat ggtgtgaaaa gtggtgtcca agcagtattt attccttctc
    12961 tttctttatt catgctgact cgtctgattg gcggaaatcg ggtgatcact ttaggaacgg
    13021 ctatcgaaca gcatttcttg gttacacaaa actgggggat gggttctaca atcggtgtga
    13081 ttttaatcat tgctatgttc atcgtgatgc tcctgacagg cgagaagaaa aaaggaggtc
    13141 gctcataaat gaaaaaattt aaatggtcgt atctttattt agggatcgtc tttttattct
    13201 tatatgcacc gatcttctat ctgatttttt attccttcaa tgaaggagga acgatgaaca
    13261 gctttacagg atttacttgg gaaaattatg aagctgtttt tgaagataca cgtttgatca
    13321 ttatcgtatt gaatacgttc atgctggctt tcttatctgc attgatcgcc acgatcatcg
    13381 ggacatttgg ggctatggga atctattaca cgaaaaaacg acgtgcacgg acagcgttgt
    13441 tgagcatgaa taacatctta ttagtctctc ctgatgttat tatcggtgct agtttcttga
    13501 ttttctttac actactagga ttcggattag gttttcagag tgtattgctt tcccatattg
    13561 ctttcagcat tccaatcgtc gtcttgatgg tcttgccaaa attgcaggaa atgaacgatt
    13621 caatggtcga tgcggctaga gaccttggtg ctaataattt ccaagtagtc aaaaatatca
    13681 ttttgcctta tctttctcca ggaatcatag ctggatattt tatggcattt acctattctc
    13741 ttgatgattt tgcggttaca tttttcgtca caggaaatgg ctttacgacg ctctcagtag
    13801 agatctattc tcgtgcaaga caaggaatca gtttagaaat caatgcgcta agtgcgttag
    13861 ttttcttgtt ctccatgata ttggtcattg gatactactt tattagtaaa gaaaaccgtc
    13921 caaaacggct gaagaaaaac agaagagtac gcgagggggt ggcacggctg cgatgaaaaa
    13981 attacaatct ctgtttttag gtattctcat cattattgcg gtgctgctgc ttggtgttcg
    14041 tcaacttgaa aaaacaagtg gcatgagtgg agcaaaggta cttaccatct ataactgggg
    14101 agattatatc gatcctagtt tgatcacgaa atttgaaaaa gaatatggat acaaagtgaa
    14161 ttatgaaaca tttgattcta atgaagcgat gttcacaaaa atccagcaag gtgggacaaa
    14221 ctacgacatc gctataccaa gtgaatatat gatccaaaaa atgatcaaag aaaaactagt
    14281 acttccatta gatcattcga agatcaaagg attaaataat attgatccac gatttttaga
    14341 cttggatttt gatccagata atcagtattc gattccatat ttttggggaa ctttagggat
    14401 cgtatataac gataaattct ttgatgcaaa tcaaattaag cattgggatg atttgtggaa
    14461 gccggaatta aaagacagtt tgatgctgat tgacggtgca cgagaagtta tggggctttc
    14521 tttgaatagt ttaggctatt ctttgaatag taagaatatg gcacagctga cagaagcagc
    14581 aaataaattg aatcatttaa caccaaatgt caaagcaatc gtggcagatg agatcaaaat
    14641 gtatatgatc aatgaagaag cctccgtggc agtgacattt tctggcgaag ctgccgatat
    14701 gatgtgggaa aatgaacatt tacattatgt gatcccacca gaaggatcca atctttggtt
    14761 tgataatatc gttatgccaa aaaccgtgaa aaataaagaa ggtgcctatg atttcatcaa
    14821 ctttatgcta gaaccggaaa atgcggcaca aaatgcagag tatattggtt attctacacc
    14881 aaatgaagaa gcaaaagcgt tattgccaaa agacatttcg tcggacgaac aattctatcc
    14941 atctgatgac acgatctccc atcttgaagt ctacaaagat ctaggatcga aatacctagg
    15001 catctataat gacctattct tagaattcaa gatgtatagg agataatggc aaaactcagt
    15061 aagaatgggg acttatcaac gttctaaaga gctaaaaatc gtcctttttc tatctgagtt
    15121 ttccaatctt ctaccaaaag cggatgaaac gccgcttttt ttgtgttatt ttattttttc
    15181 taccacgttt ttctaccaga gaaaagtgga caaaaccatt gtaaaatagg gcccgagacg
    15241 ggctagtaaa tctaagattg aaaaagttac agaaggataa agtaattg
//
LOCUS       DAZKCY010000063        14217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000063 DAZKCY010000000
VERSION     DAZKCY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.063"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1598)
                     /locus_tag="ACG8XC_001752"
     CDS             complement(51..1598)
                     /locus_tag="ACG8XC_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP9191447.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG8XC_001753"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG8XC_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9191448.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="ACG8XC_001754"
     CDS             complement(2043..2237)
                     /locus_tag="ACG8XC_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191449.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(2463..3665)
                     /locus_tag="ACG8XC_001755"
     CDS             complement(2463..3665)
                     /locus_tag="ACG8XC_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9191450.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(3668..3862)
                     /locus_tag="ACG8XC_001756"
     CDS             complement(3668..3862)
                     /locus_tag="ACG8XC_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP9191451.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(3909..4757)
                     /locus_tag="ACG8XC_001757"
     CDS             complement(3909..4757)
                     /locus_tag="ACG8XC_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFP9191452.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(4961..5311)
                     /locus_tag="ACG8XC_001758"
     CDS             complement(4961..5311)
                     /locus_tag="ACG8XC_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191453.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            5489..6352
                     /locus_tag="ACG8XC_001759"
     CDS             5489..6352
                     /locus_tag="ACG8XC_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9191454.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            6976..7968
                     /locus_tag="ACG8XC_001760"
     CDS             6976..7968
                     /locus_tag="ACG8XC_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9191455.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            7984..8868
                     /locus_tag="ACG8XC_001761"
     CDS             7984..8868
                     /locus_tag="ACG8XC_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9191456.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            8917..10524
                     /locus_tag="ACG8XC_001762"
     CDS             8917..10524
                     /locus_tag="ACG8XC_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9191457.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            10525..12009
                     /locus_tag="ACG8XC_001763"
     CDS             10525..12009
                     /locus_tag="ACG8XC_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFP9191458.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            12186..13178
                     /locus_tag="ACG8XC_001764"
     CDS             12186..13178
                     /locus_tag="ACG8XC_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9191459.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            13230..14099
                     /locus_tag="ACG8XC_001765"
     CDS             13230..14099
                     /locus_tag="ACG8XC_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9191460.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         4340 a         2426 c         2881 g         4570 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgcttacg ttgaaaaacg ttgatttatc
     2341 ggcattcccc atccatatta tatttacgtc ttattcccac tcaatatgag tagcctcgta
     2401 aagaagctat tatatcagtg ctttttcagc ctattcctca ttttggtagg gaaatggtag
     2461 ggttagattt ttactgcttg atttccatta aagtttgcaa ctttagattt tgttgcacag
     2521 gtaaatttaa tgtttcctgt catttgttct gcaattcctt catcattacg gctccataga
     2581 tgagcgtagt gctttaaagt gatttcaggg ctagaatggc ctaaccgttg cgacacgacc
     2641 agaatgtctg cgttaaactc attgattagg tagctaacat gagaatgacg taatcctttt
     2701 ccttgtatta ggggtacctt agctagtttg gcataacgct gaataattcg ttgaacagtt
     2761 gaacgataga gtgggaggtc agtgtagcta agaataaatt gttctacacc atgatttttt
     2821 tgaataactt tccacttttt tagcacttca attgtatcat cgtccagaga aatgatccgc
     2881 aagccgcttt cagtttttgt gtaaggtttc cgaataaaat ctttttgatt tgtcatttcc
     2941 aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca cgtcattcca attaagtgct
     3001 aatccttcgc ttacacgaac accagtcatg taatatagcc agatcattaa aaaacacata
     3061 tgttcgtaga aatcatcttt ataaattttt gccaaaactt cttcaaattc ttcttttgtc
     3121 cagtaaggaa cgattgattt tccttttgga attgcttttg ttcttttgga aatattctca
     3181 ttcagaaatt gcagggtgac cgcatagtct aatgatttac gaaacatacc gtacataagt
     3241 gaactgtatg cttgtgagta tccgctatcg ttcaataacc aaattcgata attttcacaa
     3301 tctcgcacat tgatttcacg aagcttctta tcaccgaaaa attcagtaat ctgaatcaat
     3361 ccacactcac gagaactcca agtacttttt tgtacgctag ctttgtagtg tggaacgtaa
     3421 gactgctcca taaattcttt gtaagttaaa tcataattcg caaatccatt tacaaccatg
     3481 tattcatttt ttatacgcat gacttctttg tgtgcttcac gagctgatga gaatcgtttt
     3541 cctagagaat cttttgttcc tttcttctgg atcctctttc cagtcacttg atctgttcct
     3601 aactcaacta gataagaaaa gcgaccattt ttatcttgat acacacaagg gtattttgtt
     3661 ttcatagtta ttcctcctgt gaaagaggaa cacctataat ttcttcaatt acttgtcggg
     3721 gtactacccc aactctttta ttattataat atggatatcc ctgatttacc attcttgttt
     3781 ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa tcctagagcc tctaaatctt
     3841 ttcgacaaat tgtatttttc attgtataaa ctcactttca aagaaaacac tatcattggg
     3901 taatagtgtt atttctttat ttttgtataa tgaacggtac tttcttgaaa atccatcact
     3961 tgttcaggac ttcccaactc atttaaagct aaagcattgt aaaaaaatgg acgtctaact
     4021 atatattcgc ttgtatatag tatatcttga ttcgtggaat catagggttt ctttaccatc
     4081 tttttggtga cagttggtag tttgagattc cgggacttga aaaaagcttt tttcttatgc
     4141 tctttcaatt ccagctcttt atcgaaatat ttgctgatat agcgcccgcg attttcgata
     4201 ctgtctacat cgatcttgtt gatcttaacg aaaccctgac cccaaatcgt agttaaccgg
     4261 ttaaatgaga taaaggggaa atcaaacaag ataatatggt agtggattgc accgcgttgc
     4321 tgcttttccc acgtggcgag atactttaaa tttgcttttt tagttttata gaggtcataa
     4381 ttcagtcgtt tgataaattt tttaaattca ttatttgcat actcaatgtc ttgaatgttt
     4441 tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa aattcgtgtc tacaaggcgg
     4501 gcaatctcaa atcgcatact ttgataatgt ttctgtttgc gttttaaact gtcatattgt
     4561 ccttgagcgg ataattcatc atagtttttt cttttttcaa gagtaatatc attttcataa
     4621 atccaagcta atttttgttt tcgttccaga tttacttcgt tttcgatcgt tttacgtttg
     4681 ggagtaatga ttgcttcata atcataaatt tcaatatacg tgggagtttc aatgattttc
     4741 tgattataag cttgcaaaaa tcatcactcc ttgttgcgta ttcgattgtc aaagaaccag
     4801 tcattttctc ctgatgttga tctataaatc aagttaaggg gaaggcctgc gtagcagccc
     4861 ttctagccta tggggtgttg ggcggtctta acggacagaa acggctctgt gggcgctgcc
     4921 ccgccgtttc gtcctaacca cccaacactc catagtatag ttagttttgt gtagtggtac
     4981 cttgttttct gggaatcaaa gttgccgtat cagcggttgc cgataaccca taactgacaa
     5041 attgtccatt cgagcgtccc caaggtacaa tcaccaaatt ggtaaatgta attatggcta
     5101 aaccttgttg ggtaagttct tccggcgtga tcacaggatg ttcagatttg actttgacac
     5161 tgattttttc gtagcgaagt tttggtagga tacattcgta gctccaacct agaatttcgc
     5221 cctccttttg ccagcgagtg acattgacga cttggtattc tttttcgagt gtttcttttg
     5281 gtaaactgag atcggtaatt tttattggca tcacaattct ccttttttat acgaaagttt
     5341 atacgatttg attaaaaaat atagatggcc atctacgact tgtattatat acgagaactt
     5401 atacgtagtc aagagatttt ctaaaaaaat atatacgatt ttttatacga gtgttataat
     5461 gaaaaaaatg ataaggagga atttgaatat gtttggttta gtacaaccta ataaagtgaa
     5521 ggtgggccaa cgaatcaaag aaataaaaga cggaatgtct ctctctttta cagaattagg
     5581 gaatcgatta ggtttaaaaa agccgacgat cagttcctac gttcaaggct atgcgttggc
     5641 tcctaagagt gtgatcaacc aattatctag tatcagcgga aaacctgtcg gctggtttta
     5701 ttttgggcat gtggaagaat atatccgaga gtatttgttt ctcattggtc agaaaaaaat
     5761 agtggacgat tatcctgaaa tcattgagaa aataaagcaa gaattttata cgggcgagtt
     5821 caaaaatcct gcttgggaga atgagctagg ctatcctttg gaggaattta tccaagaatg
     5881 ttttgtagaa tattcgagtg aaatcatgaa ggactatgtc gtacagatta cgaaagaggt
     5941 acttgaagaa tccaaaacgc taaacaaact atcatcaaaa gcaaaagaag aagctgtcac
     6001 gattctttca tctgggatca ttgactatat cgagatgagt ggtgagatca agtatggaga
     6061 gaaagatcgc attgcgaaaa tggtgaaaga atcaataaaa acctttgatg tgaaacaaga
     6121 cctgacattt aatgattcct atttgattgg aaagttgatc aatattcttg gtacagatga
     6181 agatacggag ctgtttcttt cttctttatc aatgatattt actgataagc atttttccac
     6241 tcgatttggt ggggaagaat tagtcgaaat atttcaatcg atgcgaccag cgttaatcaa
     6301 actctatcgg gagaaaacac cagatgaatt ttatgattgg tttgagaagt aaaataagaa
     6361 aagtggctta gattacaagt tggtgaaaaa atttttttca gcataacatc agtaatctat
     6421 aaaggggttg ttgattatac tgtagaagtt aagtactcaa gagatttttt gtacgtaaac
     6481 aaagcaaaaa agggtgcagt ttcactagaa aatgtactct tttttgtgat ttttcttagt
     6541 tatagagttt ttttgaaact ttaaaaatga gaaaacatat tgattttaaa gagtgtagac
     6601 atagcttctt ttgctaaata gattaaactt actggtgagt ggtcttgaac atacttttaa
     6661 atcacatttt actgagtttt attggatttt ataattcatt ggaaacctcg tacttaatct
     6721 gtttttatct atcgactaga atctgtatat attccagcta tcaatgaggc cgcagaaaag
     6781 ggtgcttaaa aataatctgt tgtttaaaat attattatca gaatacttag atacaaaatt
     6841 ctggcacaaa gtaaaaaaaa tattgactta aaaaaatgga tatggtaatc tataaacgaa
     6901 aatagtggta acgtttccaa aatgcatctt cgttaaattt ttattttttt aaacgaaaat
     6961 ggaaacgttt ctaaaatgta ttgggaggag attgaattgg aaaagacatt gggaccaggt
     7021 ggtgagataa gggcggtaca acgggataaa aaaaatattt cgaagtggga aaaattaaag
     7081 aaacagttgc ggcgtgataa gtggatgtac ttgttgctct taccaggtat catcatgacg
     7141 tttatcttta agtatgtgcc aatgtacggt gctgtgattg catttaaaga ctataatcct
     7201 atgctaggaa ttttggaaag tccttgggta ggactagatc actttaggga atttatcagt
     7261 tcaccaaatt ttacgctttt gctgagcaac acattaaaaa ttagtattta tggtttattg
     7321 ttaggttttt ttccaccaat tattttagca ctgagcttga atcaaattat caataaaaaa
     7381 ataaaacaga aaatccagtt gattctatat gcgccgaatt ttatctcagt agttattatt
     7441 gtggggatgc tgtttttgtt cctatccgca aatggaccta ttaatgggtt agtagaaaag
     7501 gttacggggc aaccaattat gttcatgtct aatccagctt atttcagaac gatttatatt
     7561 ttatcgggaa tttggcaagg aatcggctgg tcatccattc tttacaccgc aacgctggcg
     7621 ggagtcagta ccgatttata tgaggcagca aatatcgatg gggcaaatat tttacaaaag
     7681 atttggcata tcgatttgcc tgcattgagg cctgtgatgg tgatcagctt catcttggcc
     7741 gccggcggta ttatgaatgt aggatatgaa aaagcctact tgatgcaaac ttcaatgaac
     7801 atccctgcct cagaaatcat ttccacttat gtatacaaag tcggcttgca atcaggtgat
     7861 tatgcctact ctgctgctgt tggtctgttc aatatgatta ttaatgtcgt tttattactg
     7921 attgtcaaca atataaccaa gcggctaaac gatggtcaag gcttgtaatg aaaggagaaa
     7981 attatgaata gtgctttatc aaaaatcgac cgtcaaatct tatggttgaa tcgagtgatt
     8041 gtaacttttt tagtcgtcat caccattgtt ccgctgattt atgtactggt agcttctttt
     8101 atggacccag ctactttgat caataaaggt ataagcttca atccgaaaga ttggacaata
     8161 cagggatatc aacgggtatt tgcagatgat tccattttac gcggttttgt aaattcgttg
     8221 ttttactctt ttagcttcag cttattgact gtatttatta cgatgatcac tgcgtatcct
     8281 ttgtctcgac ctaacttggc ggggaaaaag attattatga ctttttttgt cattacaatg
     8341 ttcgtgggtg gtggcttgat acctacctat ttattggtaa agaacttggg catgttagat
     8401 agtgtatggg cgattatcgt accaggtgct ataaatgtgt ggaatattat cttagcccgt
     8461 acctatttta gtgctttgcc aaaagaactt tctgaagctg ctactatcga tggcgccaat
     8521 gacttgcaaa tactgttcaa agtcatgctt ccattagcga aaccaattat gttcgtattg
     8581 tttttatatg cctttgttgg gcaatggaac tcatactttg atgccatgat ttacatcaaa
     8641 gatcccgact tacagccgtt gcaactcgta ttaagaaata ttttgatcca aaaccaacct
     8701 tcacaagaca tggtgggagc caatactgcc atggcagaaa tgaaacaaat tgctgagttg
     8761 atcaaatacg caacgatcgt catttctagt ctgccattaa tcataatgta tcctttcttc
     8821 caaaaatatt ttgataaagg tgttttagtt ggttctgtta aagggtaagt attgttcact
     8881 atttgtatga ataaaaatta taaaggagat tttaagatga aagcaaaatg gatgctgcca
     8941 atcgttttta cttccgtact aattttgggg gcttgtggag gctcaaaaga aaagagtaaa
     9001 tcaagccctg attatgaact agaaaatgtc tcttttccat tgaaagaaaa agtatcttta
     9061 aaaatgatgt ctcaaagtgc ccctttggct cccaatgatc ctaatgacaa attgattttc
     9121 caacgattag aagaagagac caatctccat attgattgga caaactacag tagtgatttt
     9181 gccgaaaaaa gaaatttgga tatcgctagt ggtgatttac cagatgccat cttcaacgcc
     9241 gctgcaggtg actatgattt attaaactgg gcggaaagtg gcgttattgt accgattgaa
     9301 gatttgatcg aagattatat gccaaatctg aagaaaattt ttgatgaaaa ccccgaatac
     9361 cgacaaatgt ctactgcacc tgatggtcac atttactcta tgccttggat tgaagaatta
     9421 ggcgaaggaa aagagtcgat tcatacagta aatgggatgg cttggatcaa caaggagtgg
     9481 ttggataatt tgggtcttga aatgccgcaa accactgatc agttgatgac tgtgttggag
     9541 gctttcaaga cgcaagatcc aaatggtaat ggtgaagcag atgaaattcc ttttagcttt
     9601 attaacaatg gaggaaacga agacttaaaa tttttaatgg gggcattcgg tctaggtgat
     9661 aatgatgacc acttagtcgt tgataacgat ggtcaggtac aatttactgc taacaaagaa
     9721 gggtataaag aagccatcaa atatttcaat gaaatgaata ataaaggtct tattgataaa
     9781 gaggctttcg aacaagattg gaatacgtat gttgccaaag ggaaagaaca taagtatggc
     9841 gtgtacttta cttgggataa agcaaatatc acgggtgcag atgatagtta tgacgtcttg
     9901 ccagtcttag cgggaccaga tggaacgaaa catgttactc gtacgaatgg catgggtttc
     9961 tcaagagatc gtttcgtaat tactagtgcg aataaaaact tggaacttac agctaggtgg
    10021 atagataaaa tgtatgaacc gttacagtct gtccaaaata actggggaac ctacggtgat
    10081 gaagcacagg cgaatatttt tgaattaaag gatggtatgt taaaacattt gcctttagag
    10141 gggacagcac cgggggaact aagacaaaaa accgaagctg gaggaccatt agcagttctt
    10201 gattcttatt acggcacagt cactactatg ccagatgatg cgaagtggcg tttagatttg
    10261 atgcatgaca cgtatttgcc atatattaca gaagacaata tctatccacg tgtcttttta
    10321 gaaaaagaag aaacagatcg attagcaaaa atcgaaacgg atatgtggga ttacatctat
    10381 cgcaaaagag cagagtggat caccggcggt aaaatcgatg aagaatggga agaatacctt
    10441 gctgaattgg aacgtgtagg tttggaagaa tggattcaaa taaaacaaac tggctatgat
    10501 aattttttga agggagcaca ataagtgtta gtagaagaaa cggcgatttt ggataaagca
    10561 aatcgattta ttgaaatgat gcgaggaaaa gttaaccctc agtatcgaca aaactatcat
    10621 ttttccgcac ccatcgggtg gataaatgac cccaatgggt ttatatatta caaagggcat
    10681 taccatctct tctatcaata ctatccttac gatagtcgat gggggcccat gcattggggg
    10741 catgcgcgca gtagtgattt ggtccattgg gaagatctac cagtagcgtt agccccagat
    10801 caaccttatg acagcggcgg gtgtttctca ggaagtgcaa ttgaaagaga cggaaaacta
    10861 tatttatttt atacaggctg tcaagaaatt gatggtatta gccatcagca tcaatgtttg
    10921 gcggtctctg aagatggtat tcattttagc aaatacagtg gtaatcctgt ggtagcagaa
    10981 aaacagctag gtgaaaatgg gctaatacaa gaatttcgtg atcctaaagt aattgttcac
    11041 aacgatggtt actatatggt agtagctact aaatctacac agtctactgg tagaatagtg
    11101 atgtttcaat cgtcagattt gtatacttgg cacttttact ctgtgctgct agaagggact
    11161 tcagagcaag gaataatgtg ggaatgtcct gatttattcc atttagacgg acgagatgtc
    11221 ttaatcatgt ctccgataca aatcaagaag aacggactgg aatatcataa tatcagttct
    11281 accatggctt gtattggtga ggtaaactgg gaaacaggtc agatgatagt tgataatttc
    11341 catgaaatgg attttggaat ggatttttat gcaccacaaa caacaagaga tcctaatggt
    11401 ggacgggtga tgattgcttg gatgcagatg tgggatcgta cattgcctac acatgagtta
    11461 ggccatcttt gggcaggctc tatgagtttg ccccgagagt taagagtgaa aaaaaatcga
    11521 ttggtacaaa aacccatctc aactgtatat agtcatttag tttatcaaca aggcagtgaa
    11581 aatattgcag tgactgacac atcagtcgta tttcgaaacg cgctgtctga ttcttcatat
    11641 ttacatctag tcgcagattt ggagaaagct gaagttttta caattgaatt actgcggtct
    11701 aagacggatt gtctcaaact agcctatgac actgctacac aggtttttga attggatcgt
    11761 cagaaactag gaagagagct tacaggggcg gaagaagacc ctctgaatgt acgaaaagtt
    11821 gaagtacctt tgattgataa tcagctcgtt cttgagattt ttagagatac ctcatcaatt
    11881 gaagtcttta ttaatgggtt agaagttatg acggctactt tctatgagct ggaaaaagct
    11941 cgagatatct tgtttcattc gatcggagaa agtcgtattc aagcttttga aacagcgagt
    12001 gtgaaataaa tgtttgattg cttaggcgat gaatcaatta tttaataggt tttgaaggat
    12061 aatgttttta gaaaagaaca gatagctaga tttcccatgg atttgctatt agtattttga
    12121 aaaaattagt aatttatgac aaaattcatt atactaaact tgtgcagaaa tgtgagggat
    12181 caactatgaa agcaacaatg aaagatgtcg caaaattagc aggtgttgga gtaggaactg
    12241 tttcccgggt attgaataac ggttctgtta aagcttctac tagagaaaaa gttgaagcag
    12301 caatgacaga gttaaactat caaccagatt tttatgcccg ggggttaaag atgaagcata
    12361 cgaacacggt tgctttgatt ctacccacgg tatggcatcc attttttggc gaatttgcct
    12421 accatgtaga aacagcatta gaaagacaaa actacaagct ttatttgtgt aactccagtg
    12481 gtaatagtga aaaagaacaa gaatatatcc agatggtcag ccaaaataaa gtagacggca
    12541 tcattggaat tacttattca gatatcgatg aatacatttc gagtaatctg ccttttgtta
    12601 gtattgaccg ttatttcact gaagaggtga tttatgtgac cgctgacaat gcggccggtg
    12661 gacgtttggc tgcaaatgaa ctttatcgcc gaggatgtca gaatgtagca tatatcggtg
    12721 ggtaccaaga aacaccgaat gaaacgaaga gtcgtcgaaa atattttgaa atcgagtgcc
    12781 gtcaactggg gatttcctat aaagcgttag atatgttaga acctttagac catctctcag
    12841 aacgtgtaac aaattttttg ttagagaatc ccgatatcga tgggattttt acgatcaacg
    12901 atccaatggc attagctgtc attgatatgc ttgaaaaaat agggaaaaac gtaccagagg
    12961 atgtccaagt tatcggattt gatggtcaaa agatgacaga aggagatcct tatctagtct
    13021 caacgattgc ccaacctaca aagcaaatgg cggaagtggc agtggcaaaa ttaattgctg
    13081 tcattaatgg agagacagta gaaaaacgta ccgtattgcc gattgaattt ggcgaaggtg
    13141 acacaacgaa acctttagtg agaaacgatt ttgcttagtg caaatacgtt tctttttttg
    13201 aaaatgagtg aatgaaaggt gtagataaaa tgttatatgg tggaatagaa gccggtggaa
    13261 caaagtttgt ctgtgggatt ggtaatgaac aattacaagt gatagaacgg aaaagttttc
    13321 ctaccacaac accagaagaa acgatggagc aagtgtttga tttctttgat aagtacaaag
    13381 gaaatatcgc atcaattggt gtaggagcat ttgggcctat tgatattcaa atcgaatcaa
    13441 gaacttacgg atatatcacc aacacaccga agttagcttg gcaaaattat cattttgtag
    13501 gagctttgga aaacgccttg tcggttccca tctattggac taccgatgta aatgctgctt
    13561 gttatggaga atatatggca gggtatggaa aaggtaaaaa tagtgttctg tattacaccg
    13621 ttggtacagg tgttggtggt ggtgctgtag tggatgggaa aatagtcgca ggttttagtc
    13681 atcctgaaat gggacatatg ctcttacaac gtcataatga agatcaattt gaaggaatct
    13741 gtccttttca tcatgattgt ttggaagggt tagcatcagg acctgcgatt gaaaaacgct
    13801 ttggaagaaa agcccagact atctcatttg atcatcctta ttggacgatt gaggcagatt
    13861 atcttgctca gtgtgtgtat aacacaacgt tactatatgc accggaagta ataattttag
    13921 gcgggggtgt aatgaaacag ccgcaactac tcgagttgat tcgccaatcg tttaaagaaa
    13981 aaatgggttc ctacgtggat acgcctactc tcgaagaata tctactaact ccaaggcttg
    14041 ctgacaatgc gggcttgatt ggctgcctag ctttagcaat aaagaaactc aatcattaga
    14101 atagtattta ttagaactat tcataacata ccggaagttt aaaagggtat tctgaaactt
    14161 aaatagtctc aattcattct tctttcttga gaatctgagt agagactttt attctgt
//
LOCUS       DAZKCY010000064        13969 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000064 DAZKCY010000000
VERSION     DAZKCY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.064"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(325..2322)
                     /gene="tkt"
                     /locus_tag="ACG8XC_001766"
     CDS             complement(325..2322)
                     /gene="tkt"
                     /locus_tag="ACG8XC_001766"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9191461.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2424..2675)
                     /locus_tag="ACG8XC_001767"
     CDS             complement(2424..2675)
                     /locus_tag="ACG8XC_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFP9191462.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2822..3445
                     /gene="lexA"
                     /locus_tag="ACG8XC_001768"
     CDS             2822..3445
                     /gene="lexA"
                     /locus_tag="ACG8XC_001768"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP9191463.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3624..3842
                     /locus_tag="ACG8XC_001769"
     CDS             3624..3842
                     /locus_tag="ACG8XC_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191464.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4130..4642)
                     /locus_tag="ACG8XC_001770"
     CDS             complement(4130..4642)
                     /locus_tag="ACG8XC_001770"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP9191465.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="ACG8XC_001771"
     CDS             complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="ACG8XC_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP9191466.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7095..7538)
                     /locus_tag="ACG8XC_001772"
     CDS             complement(7095..7538)
                     /locus_tag="ACG8XC_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFP9191467.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7539..8327)
                     /locus_tag="ACG8XC_001773"
     CDS             complement(7539..8327)
                     /locus_tag="ACG8XC_001773"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9191468.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8324..9265)
                     /gene="rnz"
                     /locus_tag="ACG8XC_001774"
     CDS             complement(8324..9265)
                     /gene="rnz"
                     /locus_tag="ACG8XC_001774"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFP9191469.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9442..10029)
                     /locus_tag="ACG8XC_001775"
     CDS             complement(9442..10029)
                     /locus_tag="ACG8XC_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFP9191470.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="ACG8XC_001776"
     CDS             complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="ACG8XC_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFP9191471.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11851..12861
                     /gene="gap"
                     /locus_tag="ACG8XC_001777"
     CDS             11851..12861
                     /gene="gap"
                     /locus_tag="ACG8XC_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9191472.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12946..13881)
                     /locus_tag="ACG8XC_001778"
     CDS             complement(12946..13881)
                     /locus_tag="ACG8XC_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9191473.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3856 a         3130 c         2455 g         4528 t
ORIGIN      
        1 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgt cgggcactgc
       61 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
      121 tcttcttaaa acaaacttaa ttaattaaat tatactttta attctcaaga aacacaaact
      181 aatccgaaga attctttgtt ttgtcattta aatgtaacat agccgaaaac gaaaagtaat
      241 attgatttat tcttctaaca cataaagaac agctgtctcc aacttgcaga cagctgttct
      301 tatcagtcaa acaattgctt ggatctatga tagttgattg tacgtattca cgacattttc
      361 tactgtaaat ccaaattctt ctaaaatttt agtgccaggg gcagatgcac cgaaatgatc
      421 gattgtaatc gttgcgccct ctgttcctac gtagcgttcc caaccaaatg atgcagcagc
      481 ttcgatagcc acacgtttct tcactgattt aggaagtaca ctttctttat attcagaaga
      541 ttgtttttca aagaggtcaa agcttggcat agaaacaaca gaaacatctt tgcctttttc
      601 agccaattct ttttgagctt tcacagccaa atcaacttca gaacctgttg caatcaaaat
      661 accttctggt gtttctcctt gtgatggaga aagaacatac gcgccttttt tcaccatttc
      721 atctgctact tcttttgtag aaggcaatac tggaaggttt tgacgactca atacaaggat
      781 agtaggcgca tctgttgtct ccatcgctac tttccaagca gcacgtgttt catttccgtc
      841 tgccggacga atcacttgaa caccaggcat gcagcgaaca gatgctagtt gttcaatcgg
      901 ttcgtgcgtt ggtccgtctt ctccgacagc aaccgagtca tgtgttaaga cgtaaacaac
      961 aggagtatgt tggattgctg ctaaacgaac tgctggtctt agataatcga caaagacaaa
     1021 gaatgttcca ccgtagatac gagttccgcc atgaagttgg ataccattca tagccgaagc
     1081 cattgcaaat tcgcggacac cgaaccagat gttgcgtcct tcataatgtt caggagtgaa
     1141 atctttatct gcagccacca ttgtattatt ggaacctgac aagtccgctg aaccacccca
     1201 gaagcttggt atagctttcg ataattcttg gatgacttct ttacttgaaa cacggcttgc
     1261 ttgactgctt ccaacttcat aagttggcaa ttctgcatcc cagttttctg gtagtttgcc
     1321 atcaaaagca tcttcaaatt gttgtgctaa ttcaggataa gcttttttat aattggcaaa
     1381 catttcattc catgcatctt cagctttttg tccttcttct atcatcgttt gatggaagcg
     1441 ggcagctact tcttctggaa cagtgaaatc tggatactcc caaccataaa cttctttagc
     1501 catcttgatg ccatcttcac caataggagc accatgaaca gcagatgttc cttctttagg
     1561 agcaccatag ccgatgactg tttttacttc gatcaatgtt ggtttgtctg tttcagcttt
     1621 tgcttcttca atcgctttag agattgcttc taggtcattt ccgtctttca ctaagatatg
     1681 ttgccagcca tatgcttcat agcgagcacc aacgttttct gtgaaagctt ttgaagttgg
     1741 accgtctagt gaaatatcgt ttgaatcgta taaaacgatc aatttcccta gtttcatatg
     1801 acctgccatc gaacttgctt cttgagatac cccttccatc aagtctccgt caccgcatat
     1861 tgcatacgta tagtgatcca tcacattaaa gttttctttg ttatacgttg ctgcaaggtg
     1921 cgcttctgcc attgccatac cgacagccat tgcaatccct tgtcctaatg gccctgtagt
     1981 ggcttctaca ccatctgtgt gatgtacttc aggatgtcct ggtgttttag aatcccattg
     2041 acggaattgt ttcaaatcat cgattgtcac ttgatagcca gctaaatgaa gcaagctata
     2101 caacattgct gacccatgtc ctgccgaaag aacgaaacgg tcgcggtcag cccagttttt
     2161 tgatgtcgtt ggatttactt tcaggtgttt tgtccataac gcatatgcca taggtgctgc
     2221 gcccatcggc aatccaggat gtcctgagtt tgctttttgg actgcttcaa tacttaatgt
     2281 acgaattgtg ttaacgccaa gttggtcaat tttgtcaaac aaaataaccc ctcctattaa
     2341 tttaggtttt ttttaccttt atttcttaca ctttcattct ataagatgtc acacgaaaaa
     2401 gcaatctttt tcagtatttt ttactaaaag ttgtcatttc ggttgtgtaa gccttttttc
     2461 ttttggattt cttttaattt ttccggggtc acgtcattgc cctcaggatc gacgactttc
     2521 attccttcga tatgatgacg cataccggaa cgaaaagctt ccaaatactc tttgcgtaag
     2581 ttgatttgtt cttgttcttc ggcttttgag agaccatttt ccttcttttt acgtgcaagt
     2641 tcattgatcc tctctatttt ttcaggtgac agcatgctca gccctccttt tactctcctt
     2701 catattatat aaaaactgaa aaaaaaacaa ctagataaac taaataaaat acgaacattc
     2761 gtttgattta gcgcttttta tatggtacta tttatttatc aagtgaaaga aggtgtacct
     2821 tgtggcccga caaactgaat caagacaaat cgaagtctta cgatatatcc atgaacaagt
     2881 aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg gaagctgttc agctttcttc
     2941 tacttctact gttcacggtc atcttgcacg gcttgaaaaa aaagggttta tccaaagaga
     3001 tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga ttgggattcg
     3061 tccaacaact attccagtac ttggcgtcgt aacagctgga gaacccattt tagcagtaga
     3121 ggatgcttca gacttctttc caatccctcc taatctttct tccgaagaag gcagtctctt
     3181 tatgctgaca attcgtggag atagtatgat caatgctggg atcttggatg gtgacaacgt
     3241 gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa tgactgccga
     3301 ggacgaagcg acttgcaaac gattctataa agaaaaaaac tttttccgtc tgcagcctga
     3361 aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct atattaggac gagtagtagg
     3421 actttatcgt agtcatatct attaattgct actaataaaa gaaaactttt taaaaagagt
     3481 ttgagacgat ggtctcaaac tcttttttcg cccgggttct tcttctttca aatttttatc
     3541 acttccctct ttacaaaccg aacaaatatt cgtatactaa atatacgaac aattgttcgc
     3601 ataaattaac gaggtgagaa ttcatgaaat caatgagacg tcttggctta gtattaactg
     3661 ttttgttttt aggtattatt attggtaatt tcggcatacg tccagcttta cttgtgtttg
     3721 ttccgttatt tactttgtgg ttcatgttgt gggatgaaaa gaagtaccgt caatctgagc
     3781 ggcgcagaat tcatgaagat catttctatc atgagcccta ctacagatcc aggcaatcat
     3841 aataaggatt gactgctaat tattcaaaag acaagaagtt aaacttttac gggggtgtga
     3901 caaaaatcgg tcacatcctt ttttcgaata aacggtgttc taaagccagt ttttcggtat
     3961 taagtcaaat tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat
     4021 ttataaaaaa atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt
     4081 taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca
     4141 ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga
     4201 tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt
     4261 tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa
     4321 tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga
     4381 tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat
     4441 aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc
     4501 gtttttcttt tgcataattt acgatttgct tcgtagcttc ccgataagcg tcaccatctg
     4561 ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg
     4621 caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg
     4681 gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgccgctgg
     4741 tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg
     4801 ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt
     4861 tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct
     4921 tgagaatgga tcagtttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa
     4981 taggcctcat caggcgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa
     5041 acttctttca gatccgctaa atcatctgga caatccataa taactagaga gttcggtttt
     5101 cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg
     5161 ataatctcag gcacgaattt caacgtagaa gaatcaaatg ctaaataaag gctaccttcc
     5221 gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc
     5281 acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa
     5341 agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca
     5401 aaagcgaccg catcaagctg actcgatgca ttggaaagga caaatttcag atgctgttgg
     5461 tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa
     5521 ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact
     5581 tcatttggca gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga
     5641 tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc
     5701 gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca
     5761 agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct
     5821 tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca
     5881 atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta
     5941 ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg
     6001 atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac
     6061 gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa
     6121 aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc
     6181 tgaagtccta tccgttctga atgatgaatg gcattgattc ctaatgaaac aaggattcga
     6241 ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat
     6301 tctacaggcg gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc
     6361 aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct
     6421 ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga
     6481 gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt
     6541 ccttcttcga tcttttcttg gtatacagat tggtttggtc catacccatg ctcaaatcga
     6601 tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt
     6661 gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt
     6721 acagcttctt ggattcggct gattgcttta tccatatcgt gcatcagata cggatcatgt
     6781 aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt
     6841 tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag
     6901 gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca
     6961 ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa
     7021 aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt
     7081 atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt
     7141 tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa
     7201 gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct
     7261 tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt
     7321 tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc
     7381 agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt
     7441 acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat
     7501 ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg
     7561 ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa
     7621 ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc
     7681 aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca
     7741 gccttatcga agaaatctgt ctcgattgga cctggattca ctgtcgtaac agctacgcca
     7801 agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca
     7861 gaataaaccg ttgattttgc agttgccatt tttccagcca tagacgcgat attaatgata
     7921 tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct
     7981 aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg
     8041 aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc
     8101 ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc
     8161 tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt
     8221 gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga
     8281 ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct
     8341 cgcgaaatgg aatctcaatg ctatcaaaat ctttcatgat cgtcgtgttt tcaaacactt
     8401 cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag
     8461 taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg
     8521 aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca
     8581 gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa
     8641 tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc
     8701 catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa
     8761 tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac
     8821 gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat
     8881 cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg
     8941 tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct
     9001 ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa
     9061 tatgatcgcc gtgtaaatgt gtaataaaaa ttttaccgat ttttctcgga cgaatagtcg
     9121 ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct
     9181 cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac
     9241 ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt
     9301 ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa
     9361 cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt
     9421 gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt
     9481 ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc
     9541 tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgggtaa tttctccgcc
     9601 taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat
     9661 cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc
     9721 ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa
     9781 aatccctagc ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga
     9841 tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg
     9901 atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt
     9961 gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg
    10021 tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgta
    10081 aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta
    10141 tcacagctcc tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct
    10201 cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa
    10261 attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa
    10321 gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat
    10381 gatcgaaatt cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg
    10441 catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag
    10501 tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg
    10561 ctgtagcatt caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg
    10621 ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta
    10681 gattttccgg tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta
    10741 gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct tcataaggat
    10801 ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat
    10861 gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca
    10921 agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttagggacga
    10981 gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag
    11041 tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta
    11101 gttcgatttt tcgctcttgt cctggttcgc cgttttcagc aatttccggt gcaggattcc
    11161 gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg
    11221 tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg
    11281 ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca
    11341 tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac
    11401 gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg
    11461 gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc
    11521 cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt
    11581 attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta
    11641 tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt
    11701 ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa
    11761 tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac
    11821 agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt
    11881 gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta
    11941 gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca atttgactct
    12001 actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc
    12061 gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac
    12121 ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg
    12181 catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc
    12241 atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt
    12301 tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga atttgggatc
    12361 gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga
    12421 cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat
    12481 tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa
    12541 ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg
    12601 aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat
    12661 ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa
    12721 ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt
    12781 aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta
    12841 gaaaaattcg ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat
    12901 ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg
    12961 gcttaaaaat tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc
    13021 atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt
    13081 atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg
    13141 ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac
    13201 ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc
    13261 tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt
    13321 cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac
    13381 accgtataga acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg
    13441 tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg
    13501 tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt
    13561 ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt
    13621 cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca
    13681 atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg
    13741 tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt
    13801 ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag
    13861 gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg
    13921 aattagtata atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAZKCY010000065        13906 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000065 DAZKCY010000000
VERSION     DAZKCY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.065"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(169..417)
                     /locus_tag="ACG8XC_001779"
     CDS             complement(169..417)
                     /locus_tag="ACG8XC_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191474.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            416..544
                     /locus_tag="ACG8XC_001780"
     CDS             416..544
                     /locus_tag="ACG8XC_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFP9191475.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            601..2295
                     /locus_tag="ACG8XC_001781"
     CDS             601..2295
                     /locus_tag="ACG8XC_001781"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFP9191476.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            2362..2568
                     /locus_tag="ACG8XC_001782"
     CDS             2362..2568
                     /locus_tag="ACG8XC_001782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191477.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            2946..4079
                     /locus_tag="ACG8XC_001783"
     CDS             2946..4079
                     /locus_tag="ACG8XC_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFP9191478.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            4110..4430
                     /locus_tag="ACG8XC_001784"
     CDS             4110..4430
                     /locus_tag="ACG8XC_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFP9191479.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(4459..4923)
                     /locus_tag="ACG8XC_001785"
     CDS             complement(4459..4923)
                     /locus_tag="ACG8XC_001785"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9191480.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(5055..6308)
                     /locus_tag="ACG8XC_001786"
     CDS             complement(5055..6308)
                     /locus_tag="ACG8XC_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9191481.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(6337..6654)
                     /locus_tag="ACG8XC_001787"
     CDS             complement(6337..6654)
                     /locus_tag="ACG8XC_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9191482.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(6680..7000)
                     /locus_tag="ACG8XC_001788"
     CDS             complement(6680..7000)
                     /locus_tag="ACG8XC_001788"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9191483.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(7464..8879)
                     /locus_tag="ACG8XC_001789"
     CDS             complement(7464..8879)
                     /locus_tag="ACG8XC_001789"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9191484.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(8896..10212)
                     /locus_tag="ACG8XC_001790"
     CDS             complement(8896..10212)
                     /locus_tag="ACG8XC_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9191485.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(10362..11060)
                     /locus_tag="ACG8XC_001791"
     CDS             complement(10362..11060)
                     /locus_tag="ACG8XC_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP9191486.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(11349..12338)
                     /locus_tag="ACG8XC_001792"
     CDS             complement(11349..12338)
                     /locus_tag="ACG8XC_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9191487.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(12418..13251)
                     /gene="msrA"
                     /locus_tag="ACG8XC_001793"
     CDS             complement(12418..13251)
                     /gene="msrA"
                     /locus_tag="ACG8XC_001793"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9191488.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(13426..>13906)
                     /locus_tag="ACG8XC_001794"
     CDS             complement(13426..>13906)
                     /locus_tag="ACG8XC_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191489.1"
                     /translation="SSKKNFNQKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIAS
                     LENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINH
                     QPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         4275 a         2832 c         2377 g         4422 t
ORIGIN      
        1 tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt aaaagttgtt
       61 ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc tccctccatc
      121 cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt agtccgtcat
      181 cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc taaaaataga
      241 accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta caggaaaatc
      301 agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca tttggcattc
      361 taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt ttttcatgag
      421 taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg ctgcatatct
      481 aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat tcgaatcaat
      541 ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg aggaatcaca
      601 atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc agcggctgtt
      661 tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc attggatgat
      721 cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg gaataaaaac
      781 catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct taattaccgt
      841 acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga gccagggatg
      901 actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga caaagcacgt
      961 ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa taataaagac
     1021 cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa actagacaat
     1081 atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa cttctggttt
     1141 atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt gcgtcgctat
     1201 atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt caatcgaaca
     1261 cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa agaacaagga
     1321 tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac accgatgcaa
     1381 gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga agaagaatac
     1441 gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac agattcagct
     1501 actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc agcttctagt
     1561 ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa attctttgct
     1621 gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca tttgttgttg
     1681 aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc atttatttca
     1741 accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt cgtgcatcat
     1801 caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta tttcctttat
     1861 ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg aaaagaaatg
     1921 ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa tattcgtgaa
     1981 aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat gccttatgct
     2041 tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc taaacattcg
     2101 accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat ctttactgaa
     2161 caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat tcctatgtcc
     2221 cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg tgccgcaaaa
     2281 aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
     2341 accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc gcagatgcaa
     2401 gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca agtaataaga
     2461 gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt accgcagatg
     2521 caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt tcgagtatga
     2581 agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt aataccgaga
     2641 aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt gaaaacaact
     2701 attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac aactatttca
     2761 atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa tacataagac
     2821 tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa gatctccttt
     2881 aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg aaaggagcat
     2941 ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga aacgtagatg
     3001 atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca gccatttgcg
     3061 gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg tatgttgtag
     3121 gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca ttgaaaaaag
     3181 ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc tgcctgaatc
     3241 acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg cttttcggat
     3301 ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga gttccttatg
     3361 cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa gttttatttc
     3421 tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta aaaaaaggcg
     3481 atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa ttcgctgtta
     3541 tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta gataaagcgg
     3601 ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct ggaacagaat
     3661 tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga atggacggat
     3721 tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc acaatctctc
     3781 cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta actggtgttt
     3841 atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat atcgctgtca
     3901 agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg attgcagaag
     3961 gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa gcagcacaag
     4021 catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg aaaccttgaa
     4081 ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat acccatgaga
     4141 tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc ttctatttcc
     4201 aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt ttctatattt
     4261 ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat ttttatttgg
     4321 ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac atttttattt
     4381 acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga gaatttttca
     4441 aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag cggtcgtccc
     4501 actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa acgtggtatg
     4561 atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc tgatttccgc
     4621 taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca gggcacaaag
     4681 atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg ctcctccgac
     4741 gatggtttct ttttcatcta agaccacata ccattgagga atactttcct tattagcgat
     4801 tccagtacga atactttcct catacacaac agcaggaact ccccattttg atgaaaacca
     4861 attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt tatatgtaac
     4921 caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag tctttatgaa
     4981 aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc acttgttctc
     5041 cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat cgtagctacc
     5101 ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc tagtacacca
     5161 cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac ccatggaggc
     5221 ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac actaactaag
     5281 accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat aggtaaccca
     5341 aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt tgttacaacc
     5401 atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc taaagttgca
     5461 cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat aaacggtgcg
     5521 ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt gattgtttgc
     5581 atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa tggtgtgata
     5641 aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg ttcttggacc
     5701 actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat tgcagcaact
     5761 aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc ttttgcaact
     5821 gccggtggca caccatcagg cattttgatg atcaattttt catttccgct taaacgtaca
     5881 aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt tgagctcata
     5941 cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc ggaaacaccg
     6001 tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa gacagaaaga
     6061 atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc accaccaaaa
     6121 attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat tactgctaat
     6181 gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc aactaaatgc
     6241 cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa tgcaacaaat
     6301 ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc gacgatacag
     6361 atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat gatcttgact
     6421 gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg tcagcatctc
     6481 tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt tttcatctgc
     6541 taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc tttttgcatt
     6601 tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt ccatttattg
     6661 tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg agtacttttt
     6721 caccattcat catgccatag tcagccatat tgattacatc taaaggaatg cctttaggtg
     6781 ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa agtaaaacgt
     6841 ctacattttt agattctagt tgattatccg catctgaagc agatacagca aagatgtctg
     6901 cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga cttgtactca
     6961 ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg ttcctttcta
     7021 tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt aaaaagttga
     7081 tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa agcgcacact
     7141 aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa aaagttgtga
     7201 cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc atctgcggta
     7261 ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt cttattactt
     7321 gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc tgaccctggg
     7381 tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag taaatacaat
     7441 gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc caattctctc
     7501 ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct tcgttcatcg
     7561 ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg tgacacacag
     7621 tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt tgctttgatc
     7681 aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac gattcctttc
     7741 tcatccatat actgttcttc atattctctt cccataccgt tttctgttat cagccaaggg
     7801 atattatgat aattttcttt tagatacatg gctatatcat acatgatttt cggatagatt
     7861 tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc ataatatcgt
     7921 tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac gcgtttagga
     7981 taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc taaatcctct
     8041 gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc ttctgtgtag
     8101 tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata catataagca
     8161 gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt taaaatacag
     8221 ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc cgttgctaaa
     8281 actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg ccagcgaaca
     8341 gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa ccagtttttt
     8401 accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa atcaactact
     8461 tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc ataatgttcc
     8521 aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc acttatatgc
     8581 tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg agcccaattg
     8641 atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc ctgctggtat
     8701 tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac aaaatgttca
     8761 tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt ccaaccttcc
     8821 gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt aattttcatg
     8881 atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt ttacaaatgg
     8941 cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg caccgatatt
     9001 tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga ccattacaac
     9061 tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag tgataagagg
     9121 cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac caaaaataac
     9181 tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt gtttcatatc
     9241 ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc cagacccgcc
     9301 catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt cttttccagc
     9361 ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc gtatcggctt
     9421 caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa acatcaatag
     9481 caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga cattgtaaat
     9541 gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag ccgcgatacc
     9601 cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat tcgttggcac
     9661 ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat aaagttcaat
     9721 agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat attgtttagc
     9781 caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa cagttggatt
     9841 aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca ctttattcag
     9901 cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac ttagtgtgac
     9961 atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga aacttatctg
    10021 tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa aagcacccgt
    10081 aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat accttaaatt
    10141 attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa aactcattcc
    10201 agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta ttagtcagcg
    10261 cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat aaaacaaaaa
    10321 ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt ttctttttct
    10381 cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat tgcatcaaac
    10441 gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat atcgatcgtc
    10501 tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga ttcattaatg
    10561 attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat cgtatgaaaa
    10621 tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc aatcttttgc
    10681 gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc caaacctgcc
    10741 atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg atcctgatct
    10801 tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact gacaaatgtc
    10861 cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac agcttctcgc
    10921 accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg cagtttatct
    10981 cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt tacttctact
    11041 agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt ttttcgtaat
    11101 tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat taatccgttc
    11161 atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa attcactcct
    11221 agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa cagacgatct
    11281 tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa agtttgatta
    11341 catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga gaaatctaaa
    11401 ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc gaccagtaca
    11461 cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa tgattgtcct
    11521 cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc aacttgttct
    11581 tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat accatgtaga
    11641 taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc tagcccttct
    11701 tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat attgtagcgc
    11761 atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat tttttttgta
    11821 tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct tacaagttgc
    11881 atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg atgatagaag
    11941 atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga aatgatggat
    12001 ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc tttgctagaa
    12061 ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc aaagtttttc
    12121 tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt aattcccatg
    12181 tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc accaaaattt
    12241 tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga tttacgatat
    12301 tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc tcatcaaatc
    12361 tttttaaaac gctttctttt ttctattttt agtttactac attttaaaga aaaggagtta
    12421 atcaaaatga ttttttcgta tatttttttg ccatttgttt tttactcgtt gatagatcag
    12481 aaattgatta cgtgcttgct ggattttttt ataacgtttc ggttgttttt tgtaaaattg
    12541 ctgatgatag ttttctgctg gccaaaacgt accggctggt tcgatttttg tgacgattgg
    12601 ttggtggaaa gttcctgaac tgtctagttt ctgttttgat tgttccgcta attctttttg
    12661 tcgttcgact tgataaaaaa tcactggacg atattgcttc cctctatctt gaaattgtcc
    12721 aaatgtatct gtgggatcag taatttgcca atagattgtc agcaactcgc tataacttat
    12781 ttctcttgtg tcataaatga tctccacagc ttccacatga cctgtgtaac caccacttac
    12841 ttgatcataa tttggttttt caacatgtcc gcccgtgtat cctgacaaaa cagataggat
    12901 cccttttttc gattcaaacg gctcgaccat acaccagaaa catccccctg caaatactgc
    12961 acgttcttga tagtcagcat cttgcatggc atgcttggaa aagtcatatt tttgctcctt
    13021 atcaaatcct tccgtaatca ataaataaaa gtccattaca tcaggcgtca gattcattcg
    13081 gatagctaat ggacgtagtg ttgcttctag ttttgataac tgttctttaa cggatatatt
    13141 ttctcttatg tcattctttg tctgaatcaa tacttttcgt tcccaatctc gtgtattcgg
    13201 attcaaaatc agattataaa gtgtttgaat gaatatttgt tcctcatcca tagattctcc
    13261 tcctttccga agaagacaag ctagtgactt tagctgtaat ttatcataaa gtgaggaaat
    13321 aagcgattac ttcatccccc tacagatttt tcgtataaaa aagagagaat ccttatcgac
    13381 agattctcct ttttaatcaa tttattttat aaatgaaaag ttaatttacc ttttatggaa
    13441 aactatttta aaaaataaac ataaatatgc agttaatgta acaagataag aaataatagt
    13501 cgttactgta taacttgtgc ttactttaat atcagcaact cctttgactc cgcttatttg
    13561 taccaatcca ttagcattca cctgctcctt tacaggttga tgatttattt ctacattcgt
    13621 tcctaaataa gcatatacag gaagtgtgat tacttggctc ttctcattag ttactctata
    13681 tatgatagaa ctaccagttg ttttataagg aaggtttacc cattgactgc caatctttgc
    13741 ttcgtgtttt attatatgct ccttattctc caatgaagcg ataggagaat aatcatataa
    13801 aaaataatcc attcctttag tggtatacgc ctctactgtt tctttgttta caacaatatg
    13861 tcccggctga gaaattattt tttgattgaa atttttttta ctagaa
//
LOCUS       DAZKCY010000066        13732 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000066 DAZKCY010000000
VERSION     DAZKCY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.066"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..810)
                     /locus_tag="ACG8XC_001795"
                     /note="aph(3')-IIIa"
     CDS             complement(16..810)
                     /locus_tag="ACG8XC_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFP9191490.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(903..1445)
                     /gene="sat4"
                     /locus_tag="ACG8XC_001796"
     CDS             complement(903..1445)
                     /gene="sat4"
                     /locus_tag="ACG8XC_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFP9191491.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            complement(1442..2350)
                     /locus_tag="ACG8XC_001797"
     CDS             complement(1442..2350)
                     /locus_tag="ACG8XC_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFP9191492.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            complement(2383..3117)
                     /locus_tag="ACG8XC_001798"
     CDS             complement(2383..3117)
                     /locus_tag="ACG8XC_001798"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9191493.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            complement(3098..3967)
                     /locus_tag="ACG8XC_001799"
     CDS             complement(3098..3967)
                     /locus_tag="ACG8XC_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP9191494.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            complement(3982..4206)
                     /locus_tag="ACG8XC_001800"
     CDS             complement(3982..4206)
                     /locus_tag="ACG8XC_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9191495.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            complement(4349..5893)
                     /locus_tag="ACG8XC_001801"
     CDS             complement(4349..5893)
                     /locus_tag="ACG8XC_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9191496.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            complement(5913..6329)
                     /locus_tag="ACG8XC_001802"
     CDS             complement(5913..6329)
                     /locus_tag="ACG8XC_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFP9191497.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            complement(6330..6827)
                     /locus_tag="ACG8XC_001803"
     CDS             complement(6330..6827)
                     /locus_tag="ACG8XC_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFP9191498.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDC
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            complement(7138..7815)
                     /locus_tag="ACG8XC_001804"
                     /pseudo
     CDS             complement(7138..7815)
                     /locus_tag="ACG8XC_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(7815..8432)
                     /locus_tag="ACG8XC_001805"
     CDS             complement(7815..8432)
                     /locus_tag="ACG8XC_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9191499.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(8446..8616)
                     /locus_tag="ACG8XC_001806"
     CDS             complement(8446..8616)
                     /locus_tag="ACG8XC_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191500.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(8964..10454)
                     /locus_tag="ACG8XC_001807"
     CDS             complement(8964..10454)
                     /locus_tag="ACG8XC_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFP9191501.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(10497..10796)
                     /locus_tag="ACG8XC_001808"
     CDS             complement(10497..10796)
                     /locus_tag="ACG8XC_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191502.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(11162..11446)
                     /locus_tag="ACG8XC_001809"
     CDS             complement(11162..11446)
                     /locus_tag="ACG8XC_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9191503.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(11965..12450)
                     /gene="vanZ-A"
                     /locus_tag="ACG8XC_001810"
     CDS             complement(11965..12450)
                     /gene="vanZ-A"
                     /locus_tag="ACG8XC_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFP9191504.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(12603..13514)
                     /locus_tag="ACG8XC_001811"
     CDS             complement(12603..13514)
                     /locus_tag="ACG8XC_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFP9191505.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3866 a         2858 c         2095 g         4913 t
ORIGIN      
        1 atctaaatct aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca
       61 atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc
      121 ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct
      181 cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag
      241 gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag
      301 ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag
      361 atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg
      421 acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat
      481 cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact
      541 catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag
      601 ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt
      661 ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac
      721 cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt
      781 caattccggt gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat
      841 ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact gttccttgca
      901 ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat
      961 aacatagtat cgacggagcc gattttgaaa ccacaattat gatagaattt acaagctata
     1021 aggttattgt cctgggtttc aagcattagt ccatgcaagt ttttatgctt tgcccattct
     1081 atagatatat tgataagcgc gctgcctatg ccttgccccc tgaaatcctt acatacggcg
     1141 atatcttcta tataagcgta ccggttccaa ttttttcgca gtttaacttt tccgacgcat
     1201 ttatcgtctt ggtagtaaag atatattatc ttatcagtat tgtcaatata ttcaaggcaa
     1261 tctgcctcct catcctcttc atcctcttcg tcttggtagc tttttaaata tggcgcttca
     1321 tagagtaatt ctgtaaaggt ccaattctcg ttttcatacc tcggtataat cttacctatc
     1381 acctcaaatg gttcgctggg tttatcgata tctttcaggt gccctgcttt catttctgta
     1441 atcactgttc ccgcctctct tctatatcag cggcatatgt gctatccagg cagccggttt
     1501 taccagtgta ttttttatac atgtccctgg tatattttgt tatattccta tcatactccg
     1561 gataggcata atgaagcctt tccgccacct caccggatac cgccctgaac aattgatggc
     1621 atagaaataa tgcttcccat atgttttcat aggaatccat ccggtaggtg gacaaaagtt
     1681 tctcccacaa atcctcggat atataccttt caataaactt atagttcttg cctacactta
     1741 atttaaagcc tgtttcgatg ccgaccttcc atgatatcat tctcagcagc tcatggcgaa
     1801 caatctgatt aaaatgatca atagcaaata aaatttcctt acggcacaat cctttaataa
     1861 cataaggtgt tacattccaa aattcattgc agcaatcatc atactccctt gcgcttggct
     1921 ttcttacatg ataatctata tcagtcggaa ctatgtccct tttaattcta caatctttat
     1981 caattagaac ctttattaat ttatcgccct ttaggtaatt atctaactct tccaagggca
     2041 ataaggtaag atcaatttta ttgtaatcat caaatagcat aagataggaa aatccctttt
     2101 cttcaggtgg gaataattcc atatcctccg gcttttgcat cattattata ttcccaaatt
     2161 gattaagcca gtcatcatta gatataaacg gttctatatc acttacaaaa tatgtaatat
     2221 cataatcctg aaattcatct ttaggtatat taatatttgc gcgtgacccc tcaagggtca
     2281 caattcgaat acgttcatcc tgttctgcta aagaaagtac taaatccatc atttcttttt
     2341 ctgatctcat ttacatactc ctcgtttatt ttttctatat tattacatct tcttttttgc
     2401 cgatacaatc agcatcattg ggcgtcgcat ttcatccgcc atccccggaa tatccatcat
     2461 gttctctggc ggctgtggct ccacaatctg atttattata aaactatttg aaagcagtgt
     2521 atttagatat gtggtcagtg ttctatgata ttttgtaacc ttttcttcca aaaacatagc
     2581 tgtccgtttg ccctcataat aataattgtc caccgggaaa tgcagtattt ctcctttttc
     2641 gttataatac cagtcttgtg ttccatgagc agtaaaaaca ggatgttcaa ctgtaaaaac
     2701 taaattgcca ccagccttca gcatcctata tatctttttt attaaattct catagtctgc
     2761 tacataatga aacgcaagcg aacttagtat tacatcaaag ctctcctctg ggaaatccac
     2821 atcttctatg gcacagcatt catattcaat ctgtggaaaa tgggtttttc cttttgctac
     2881 ttcgagcatt ttatgagaaa tatcaacacc tactacagag gaagcaccgt tttccatcgc
     2941 atatatacag tgccatccat agccgcatcc taaatcaagc acacgcttac ccttaaaatc
     3001 aggtagcatc tttttcaaag tctcccattc tcccgcacca gccagtcctt tctgcgagcg
     3061 actcatttga ctgtattttt gaaaaaatat attatcatca tatttgtttt ctttcatctg
     3121 aactctcctc gtttaaaaag ttatttatct ccgatacaat ttcattcact tctttataaa
     3181 gtttctcggt catgtcgtag cattcaaaaa gagaaagacc gagtacttca aaaatatgat
     3241 taactctttc ggcatatttt tcaggtttat attcaaaagt ttcaagcagt tttatagctt
     3301 tcttttcgtt aatgcaataa gcattattac atgcaaatag tacttgattt aagcatgaaa
     3361 taatacgaaa aacatggcct gcaatataat atttatcctc tgcccccgca tttgctttta
     3421 caaacattaa agagaattca gcttcaaata taaaaaagtt catcaagctt ttctttagag
     3481 cagtagggta aatttctgcc tgctttttta attcgcataa gctttcgttc ttagcatata
     3541 gtattttact aatcgctaat tctccacgat acatagcact tatataacca tggggatgcc
     3601 cggtctgata attggcagta acaattcctt gctccgtatc tttgattatt tgttccaccc
     3661 gttttatatc acgtaatatc aagtcaacat gacacccgtt tataactaac catccaccgc
     3721 cattaaccca atcaccccac gctccgggag gtacaacaag gttgtttcta ttctcatcat
     3781 ccaactctgt agcaatttga ttaatcgctg tcaggtcaaa tgagtctgaa ttgtaataga
     3841 ttccgatatc tatatcagaa ttttctgtat gggtgcctct tgcacgtgag ccccctaata
     3901 cgatgccttc tataaaaggc aaagatgata atttctctgt tactatttga ataacattac
     3961 ctaccatata aacatcctcc tttattcgtt agatattttt atttcttctc catctggtaa
     4021 aataaatgcc tgcacaaact tgacacctag cacatcagca atggccttca actcatccaa
     4081 ggttactgtt tctcgttgta attttttatt gaaattctgt ggagtctggc caatacgtct
     4141 agcaagttcg gaaacactta tattcatttg ctcacacagt tctttaatca tttctgccgt
     4201 agtcatacag cacctccaag tttacgctaa gaatattata aaccatttgg ttgataaaca
     4261 caatagttat taaaatatta ttataatcag caagcaaaag aaaacccaca actctactgt
     4321 agagtcatgg gctatttact tctggttttc atatttccat ttcgatgcca gatttgaagg
     4381 ctatgacgaa gtggccttca tagactgtaa cgctctggat tatcttcctt acaagcttat
     4441 catcatattc taaggtgcgg aatttgttct tgcggataaa ttcaatcagc tcattgattc
     4501 gctcgttctc gccactgagg gaggcatctt ctactaaaag gatctgtcgc ttgtctctca
     4561 gctcatcaat ctcatctgct aagtggtcat agtcttgacc cttgttcgcc agcttgatta
     4621 gttctttttg tttttcctct agtgagttgt taatctctga aatttggtat tctgtggttt
     4681 cgccaatcac agcatgaata ttctcttcca gtgtctttat catattgttc ccacctgcaa
     4741 gtagcttatt aatcgcagtc attactgcac cataaagttc atcttctttt acggttcggt
     4801 tcttacaaac ttcaggacct tgctcgattc tagtcacgca tcgccagaca aattcttttc
     4861 ttccgtggat attccaatag acccgtctat aaatatcgcc acaatctcca cagaaggtga
     4921 tggcacttaa agcgtacttg ctactgtaaa ttcttttgtt cttgccttct cctgtgtaga
     4981 tattgcttct ccgatgaatt tcttcttgcg cctgcaaaaa taactcttta ggaataatcg
     5041 cttcatggct attttcaaca taatattggg gaacatgccc ttcatttttc actcgtttct
     5101 tggtcagaaa atctactgtg acagtctttt gcagaagggc atctccgatg tatttttcgt
     5161 tttgaagtat cttcttaact gattctggtc gccatcttgg ttttcccgct gctgttaaaa
     5221 taccatcctt ttcaagggct cgaccaatgc ccactagact ctgaccctca aggtattctc
     5281 ggtagatgcg tttgataatc tcagcttctt cggggacaat agttaaattt ccatcttcat
     5341 ctttcgtgta gcccataaat cgcttatggt tgacctgcac ctttccttgt tggtatcggt
     5401 actgtagccc aagcttaacg ttttgtgaaa ggctctggct ttcctgctgt gccaaagatg
     5461 ccataatagt cagcaaaacc tcacctttgg catccgtggt gttgatgttc tctttctcaa
     5521 aataaactga tatgttctta tccttgagct gtctaatgta ttgaaggcag tctagggtgt
     5581 tacgcgcaaa tcggctgatg gattttgtaa taaccatgtc gatgttaccg tccatacact
     5641 ctgcaatcat gcgattaaat tcgtcacgct ttttagtgtt cgtaccagag ataccatcat
     5701 ctgcaaagat gccagcaaac tcccattcat tatttttctt tataaactct gtatagtgtg
     5761 agacctgaac ctcatagcta gaattttgtt cttctgtttc tgtagaaaca cggcaatagg
     5821 cagcaacacg aagtttcttt atcttttctt ttgcggctgt acttcctact cttttacgag
     5881 caggaataac cattatattt ttctctgtca ctttattcct cgctttctat cagactgtat
     5941 aagtattctg ctcgttcaaa aggatccact ggcatcttat tatctgcctt tttcattttg
     6001 aatcgttctt tgggaggggg agaggtgaaa gcggcaagct ctaccactcg tcctaaatcc
     6061 tttgcacgtt tatctctaac ttcttcagct ttatcaaata tctctttatc aataattgct
     6121 ggatacgtat catttccaag gtagttgacg tttttcaaaa tgcgccccat cacagaatgt
     6181 gtcttttcaa tacctgcctg ttcgccagcc actgtaaggg atagtcctga tatgtatttc
     6241 tcaaagaata cctttacttg acctgctgcc ttttcatcga cagtaaccac tccatcttga
     6301 attttgtatc catatggaat atatgccatt tatctcacca ccttttcttt cagggaaaga
     6361 ccgcatttca aattgaatgt cagctcatcc ctggaattta caatgatgtt cactacaaat
     6421 tcttcaaata gttcttccgt gtagtcacca ttaaaattat ctgctgacac ataattaatg
     6481 aggtccttta tctcgtttgc tcgcaaaacc ccactcgcag agttcgttac caggtttgtt
     6541 ttttcggtag taagattttt aatctcacta tctaaaacat ttcgttcctg attaaaaaga
     6601 gcaggttcta ggaaaccttt ggacatcagt gcaataaggg tattgcgttc ttccaatagt
     6661 tgctccatta gcttatcaat agcgtccatt ctttcacagt cgctttcttc atcgatttgg
     6721 ctaactgact tgaaaagtgg ttctaggatt agcttatgac tgaaagcaag cttattcatc
     6781 atggttgtca atgtggcttt gatttcccca tctcgcaaaa acaacatgga gcaactttct
     6841 ttatcttcga tactatctca acatgtgtcg tggataaggt aagtatgtaa aatggtatgt
     6901 ggaacatagt gatttgctgt aaatgatttg ctaactactt tagttcttta tacaaagtat
     6961 aaatcggata ttttattatt ttttctttta tatcttctga gattaggtca aagtatttgt
     7021 ccgctaaatc agaaaggggt tcttttttca tcacctaatg ggtgatgaag cagaatactt
     7081 taaagactat taaatcaatg ttaggaaaga ctattagtaa aactatgaat tattcaggaa
     7141 aaaaggttct tttttaaagt tttctattgc ttcctggcgt tgataaatga gatataaagt
     7201 cggtgtttgt acacgtccta atgaaaatgt accttgtacg cctttttgtt gtaaattcaa
     7261 ggtatataaa gggcttgcgt tcatgccgat caaccaatcg gcaatttggc gtgtttgcgc
     7321 ttcttgataa aagggatagt aattcattcc tggttgcaaa ttttgaaaac cgcttcggat
     7381 cacatctttt tctaagctat tgatccataa tcttttatac gttttatctt tagaaaaggc
     7441 atttgcttta tggataatcg accaggcgat attttcacct tctctgtcgc tatctgttgc
     7501 gacaataatt gtatttgcct ttttgagaag ttcagcaaca attttaaact gctttccctt
     7561 atcttttgca acttcaaaat cgtatcgatc aggaaaaatc ggcaaagatt caagtttcca
     7621 attttgccac ttttcgtcat aatgacctgg ttctgctaat tccactaaat gcccaaaacc
     7681 aaaggtgata aacgtttcat ctgtaaatag tgggtctttg atctcaaaat aaccgtcttt
     7741 tttggtgctt tgttttaaag cacttgcgta ggctaatgcc tggcttggtt tttcagctaa
     7801 aataaccgta ctcattaact atccctcttt tcattgtttt ttctttgatc gactgtcacg
     7861 ttatatcttg ctcgatacct tctaaacgtt cggcgattga ttccagtttg ttcttcaact
     7921 tctttatcgg ataaaccatt caaaaacaaa tcgaaagcat gctttaaacg tggatcattt
     7981 tctttaaatt tatgctgacg acctttgaat ttgccttttt tcttagcaat ttcgattcct
     8041 tgtgcctgac gttccttaat tttttttcgt tctgattctg cttgatactt gtacaattca
     8101 atgacaaggc tattaatcaa acgccttaaa ttttcatctt caataccatt cattgagggt
     8161 aaatttaaga cttccagggt tgccccctta atttgaattt gattcatcaa ttctgttaat
     8221 tctttattat ttcgtcctaa tcgatctaat tcagtaacaa taacaatatc cccttcacga
     8281 atatagttaa gcatagcttg taattgtggg cgttcgaccg attgaccgct taatttgtct
     8341 gaaaagacct tagaaacgcc ctgtaacgct tgtaattgcc gatctaagtt ctgttctttg
     8401 ctactgacac gtgcataacc aattttagcc attttcaacc aacctctaaa attctctcgg
     8461 ttgcaataac caatcagcaa tatctacttt ttcaatttca aattgcttat cagaaattgt
     8521 cttttcgtaa gcgataaaat cttgcgcata ttgttgctca ttaaaaatag ccaccacttc
     8581 gtcattttct aaaactcgat aaataaattt tttcatttta ctcctcctat tatgcccaac
     8641 ttaaatgacc tattcaccaa gtcaattata ctgctaaaat catattagga caaataggta
     8701 tactctaatg acctataaaa gatagaaaat taaaaaatca agtgttcgct tcgctctcac
     8761 tgcccctcga cgttttagta gcctttccct cacttcgttc agtccaagcc aactaaaagt
     8821 tttcgggcta ctctctcctt ctccccctaa taattaatta aaatcttact ctgtatattt
     8881 ctgctaatca ttcgctaaac agcaaagaaa aaacaaacac gtatcataga tataaatgta
     8941 atggcatagt gcgggtttta ttttcagcct gtatcatagc taaacaaatc gagttgtgtg
     9001 tccgttttag ggcgttctgc tagcttgttt aaagtctctt gaatgaatgt atgctctaag
     9061 tcaaaagaat ttgtcagcgc ctttatatag ctttcttttt cttctttttt tactttaatg
     9121 atcgatagca acaatgattt aacactagca agttgaatgc caccatttct tcctggttta
     9181 atcttaaaga aaatttcctg attcgccttc agtaccttca gcaatttatc taatgtccgt
     9241 tcaggaatgc ctagcacttc tctaatctct tttttggtcg tcactaaata aggcttgtat
     9301 acatcgcttt tttcgctaat ataagccatt aaatcttctt tccattctga caaatgaaca
     9361 cgttgacgtt cgcttctttt tttcttgaat ttaaaccacc cttgacggac aaataaatct
     9421 ttactggtta aatcacttga tacccaagct ttgcaaagaa tggtaatgta ttccctatta
     9481 gccccttgat agttttctga ataggcactt ctaacaattt tgattacttc tttttcttct
     9541 aagggttgat ctaatcgatt attaaactca aacatattat attcgcacgt ttcgattgaa
     9601 tagcctgaac taaagtaggc taaagagagg gtaaacatga cgttattacg ccctattaaa
     9661 cccttttctc ctgaaaattt cgtttcgtgc aataagagat taaaccaggg ttcatctact
     9721 tgttttttgc cttctgtacc gcttaaaacc gttagacttg aacgagtaaa gcccttatta
     9781 tctgtttgtt tgaaagacca atcttgccat tctttgaaag aataacggta attaggatca
     9841 aaaaattcta cattgtccgt tcttggtatg cgagcaatac caaaatgatt acacgttaga
     9901 tcaactggca aagactttcc aaaatattct cggatatttt gcgaaattat tttggctgct
     9961 ttgacagatt taaattctga ttttgaagtc acatagactg gcgtttctaa aacaaaatat
    10021 gcttgataac ctttatcaga tttgataatc atagtaggca taaaacctaa atcaatagcg
    10081 gttgttaaaa tatcgcttgc tgaaatagtt tcttttgccg tgtgaatatc aaaatcaata
    10141 aagaaggtat tgatttgtct taaattgttt tcagaatgtc ctttcgtgta tgaacggttt
    10201 tcgtctgcat acgttccata acgataaacg ttgggtgtcc aatgtgtaaa tgtatcttga
    10261 ttttcttgaa tcgcttcctc ggaagtcaga acaacaccac gaccgccaat catgcttgat
    10321 tttgagcgat acgcaaaaat agcccctttg cttttacctg gcttggtagt gattgagcga
    10381 attttactat ttttaaattt gtactttaac aagccgtcat gaagcacagt ttctacaaca
    10441 aaagggatat tcattcagct gttctccttt cctataaatc ctataaaata ggttgtttaa
    10501 ttaacttggt ttgctttttc attcaactgt ttcaatattg catgttttga aaaagatttt
    10561 tttcctttat aagtcaattt ttttccacta atcgaataaa ttattttgtt attttctatt
    10621 aacttatata tataatcttc cccctccgaa gaaaaatact tatctgattt tgtttctaag
    10681 tagatatttc tcttttctaa ctctttctta aacgtttcta gtgtatagat atttgctaat
    10741 tttcttatct ccaataaact attttttata taagttttac attcatcatg attcatacaa
    10801 actccacctt ctataaatga atacaaaaaa agcaatcaaa cgatttccga ttgattgctt
    10861 aacaattctt aaattcagta gcttagatac ttgaaaactc tctgatttcc ctatataatg
    10921 atagtacggt tatataccgt cttcaaacaa agttaattaa ataacttctt acgagggaag
    10981 agttcatctg actaactgat aagcgttggt ttggcaatct tatcgggcta tgcatttata
    11041 aaatgtcgtc aaacatttta taaatgtgtc atggctcttt tttcgtttct attcagttcg
    11101 ttgtttcgtt atatctagta taccgctttt aaaaaaaata agcaacgatt tcgtgcatta
    11161 ttcacacgaa gtcattgctt ttttcttctt ccatttctaa atccaatgtt acttgttctg
    11221 attctgtttc tggttctggt tctgttggct catttgggat taaatccact actagcgttg
    11281 agttagttaa ctttgcaatt tgttctagtg tttttatggt tggatctgat tttcctgatt
    11341 ctattcgtga ataatttgat ctactcattt ctaattcttg ggagaatttt tcttttgatt
    11401 tggtacctct catctttttt aagctttctc taaatgctag ttccaagttt ctcccccctc
    11461 tttttgtgta ataaaattca cgctttattt aaattatagc ttttaatatc ttaataaaca
    11521 agtgattaat cacttgtgat ttgtgattat tccaaaagta ggtaatttca ccaacctttt
    11581 tcaaaagttt ttatcgacga ttattggact ggatcgtgtt tcctaaagcg attctaagcg
    11641 catgtttaat tttaaagtgg gtaattctct ttaaaacgtc ctacaaagat tcaaaaaaag
    11701 catggctgct agtgggggta ccgtcaggaa aatgcggatt tacaacgtta aggaaattta
    11761 ttaaagagac caattcctca gcactaaaat tgataattgg gctttctttg ttactatagt
    11821 gttaggtaag ctttttactt tatcaccggt tttcccctca cttcacacct acgggcgagt
    11881 ttccccacat cccgttttcc atctaatcca attcattcaa tatctttatg tgtatgaaag
    11941 atcagattgc ttgatttaga catcttacac gtaatttatt cttaaatggg tacggtaaac
    12001 gagcaataat acgagcaaaa gtatacctac aaaaataata actctgtcta attttttttg
    12061 attcatatgc ttattgctta aaccatataa tttcagtcca agaaagcctc caacagtatt
    12121 tgtaattaca tctgttatgt ctgtcgctcc aatagcgaag ataaattgaa ttatttcaaa
    12181 agtaagactt aaaaccagta caaaagcaaa cttaggtaaa aatccgattt ctttaaaatt
    12241 gacattcaaa agcaagccaa atggaataaa gattataaca ttatctatca tctctctgaa
    12301 attcccagta gcagtaaatg gagtcaagtt aagacttctt tgatgataat taaatactga
    12361 taaaatattg tattgtaatt tgaataacac taaccagatt agtgtcacta aatataaagc
    12421 tagcaatcct ctagataata tttttcccaa tataatttcc tctaattctt ttgtttaagt
    12481 tgtatcatta gtatagcaat gtcctagtta acgtgggttt taaattaaaa agctaacgtt
    12541 accccaaaag tgctagtaca ataatgctgt tcattaattt cgttggtttc attccgccat
    12601 ccttacctcc ttgaattagt atgtgttgat ccgggaaaca ctgtcacaat tacaccgtct
    12661 atattgtttc ctgatatctc ataacgaaga ttagtcggca catgaatggt ggtattttta
    12721 gtaacaggat aataaaagat ctcatatttt tccccattta cactaacaga aatggttttt
    12781 tcttctttta ggtaatccat atattcctcg agaacgaaat tcttttcttt cataatcgca
    12841 ctatgtggta aaccaacata gcgaatatgc catggttcat attgaattcc tgttaactct
    12901 gttttgtcct ctggataacg taaaatgaac ccgtatttcc aagcattttc ttctatccac
    12961 tttccttcag gggctcgttc cattttcgtc aagcttgatc ctacatctag tgataaacct
    13021 gaattatgct cactataacc tgctggtaag gcatactcag cccccatttc ttggtaaagc
    13081 acactttgct catcaaagtc tcgatagcca ctattaataa taaaatgact aacgccaccc
    13141 tttacagcat cattgaccat ctctgaaaat ttttgtgcta tttcttttga catataaata
    13201 ttactatcaa gcaacccgta tccatttatt aattcgtcat gtttagataa attcacgata
    13261 tctgacttca cactttcttg gcgaacagga tatttactat tgattaatag cagatttcct
    13321 tgataaacct gttcttctgt aattgttttc tcttgggtat tttcagaagt cccactattt
    13381 tctaaatgtt ctttgggatt ttgatcataa ttttgaaatt cgactttttc ctgagaaaac
    13441 agtgcttcat taacgtagtc ataacctaag tatattaaga ataacaataa caataaaaaa
    13501 aacaacttct tcattttcag tctcctcctt aactatatag ttggaagtat gaaatatttc
    13561 tagaatcttc cccttaaacg gcacaatctc gtttgagatt gtgccgtttt gccccgtaaa
    13621 ttacagtcaa ttatttcgcc ctactggatt tactattatc accaatgtag atgaattttc
    13681 ataagtgata cccatgctga atttaacgag gctgaactgc ctgttgacaa tg
//
LOCUS       DAZKCY010000067        13724 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000067 DAZKCY010000000
VERSION     DAZKCY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.067"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..903)
                     /locus_tag="ACG8XC_001812"
     CDS             complement(<1..903)
                     /locus_tag="ACG8XC_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFP9191506.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSES"
     regulatory      complement(927..971)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1186..2154
                     /locus_tag="ACG8XC_001813"
     CDS             1186..2154
                     /locus_tag="ACG8XC_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9191507.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2267..2545
                     /locus_tag="ACG8XC_001814"
     CDS             2267..2545
                     /locus_tag="ACG8XC_001814"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9191508.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2663..2899
                     /gene="secG"
                     /locus_tag="ACG8XC_001815"
     CDS             2663..2899
                     /gene="secG"
                     /locus_tag="ACG8XC_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP9191509.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3002..3757
                     /locus_tag="ACG8XC_001816"
     CDS             3002..3757
                     /locus_tag="ACG8XC_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9191510.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3760..6117
                     /gene="rnr"
                     /locus_tag="ACG8XC_001817"
     CDS             3760..6117
                     /gene="rnr"
                     /locus_tag="ACG8XC_001817"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP9191511.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6139..6603
                     /gene="smpB"
                     /locus_tag="ACG8XC_001818"
     CDS             6139..6603
                     /gene="smpB"
                     /locus_tag="ACG8XC_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP9191512.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6905..7624
                     /gene="atpB"
                     /locus_tag="ACG8XC_001819"
     CDS             6905..7624
                     /gene="atpB"
                     /locus_tag="ACG8XC_001819"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP9191513.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7677..7892
                     /gene="atpE"
                     /locus_tag="ACG8XC_001820"
     CDS             7677..7892
                     /gene="atpE"
                     /locus_tag="ACG8XC_001820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFP9191514.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7983..8507
                     /gene="atpF"
                     /locus_tag="ACG8XC_001821"
     CDS             7983..8507
                     /gene="atpF"
                     /locus_tag="ACG8XC_001821"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP9191515.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8494..9036
                     /locus_tag="ACG8XC_001822"
     CDS             8494..9036
                     /locus_tag="ACG8XC_001822"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP9191516.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9059..10615
                     /gene="atpA"
                     /locus_tag="ACG8XC_001823"
     CDS             9059..10615
                     /gene="atpA"
                     /locus_tag="ACG8XC_001823"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP9191517.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            10629..11531
                     /locus_tag="ACG8XC_001824"
     CDS             10629..11531
                     /locus_tag="ACG8XC_001824"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP9191518.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11554..12960
                     /gene="atpD"
                     /locus_tag="ACG8XC_001825"
     CDS             11554..12960
                     /gene="atpD"
                     /locus_tag="ACG8XC_001825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP9191519.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12974..13396
                     /locus_tag="ACG8XC_001826"
     CDS             12974..13396
                     /locus_tag="ACG8XC_001826"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP9191520.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4541 a         2449 c         2987 g         3747 t
ORIGIN      
        1 actttcggaa aaaagacgag tatctacttt tgtcataatt ttactgttag caggtttctg
       61 ccaaaattca taacgatcaa ccaatgtttg tccacgactt attccctgag tttccacagc
      121 ggtgaagctt ttgaaacctt cacagatggc cccctcgatg aaataagcaa cagctaacgg
      181 atcattgatg acacatccta aaattctttc ttgtttccag tggaagtcaa aataaaaacg
      241 agtgatcgct tttaaatact ctccctcttc aggattcatt tgacagcagt attcgaggat
      301 agtcggtgtc aagacgattt ctcttgtcac atctaaccca accatttcta tcgtttgttt
      361 gagattctcg aatacataag aagctgcttc aggatcacac cagtaattgt attcagcaac
      421 tggcgaacag ttaccatggc ttttgtaagt tccacccatc gagacaaaac gatccatatg
      481 tttcccaata tttgggttga ctttcaaagc aaaagcgata ttggtcaaag gacccaaagc
      541 aatgactgat gtatttgtct tttctttgaa ataatcagcc aaaaaatcaa cggcatggag
      601 aggttctgcc tgttttctca agatcatcgg gaaatttgtt tcccctaaac catccatacc
      661 atgagtgtct tgtgcactga tgaaaggttg cttcaaaggt ttttccattc cttgataaac
      721 aggaatgtct aaacgatcac agcgttccag gcatttcagc acattctgcg tacctatatc
      781 tgcagggaca ttcccacaaa ctacggtaat agcaactact tcgatatcag gggatttgac
      841 agctaatgat aaagctaaag tgtcgtcgat ccctggatcg caatcaataa ttacttttcg
      901 cattatttcc aatctccttt ctttagagat acttagtttt ttattctggg aagtttccga
      961 accagtccaa gacgagcgtc ttgattataa tgcacgaaat tagtatactg gcttttattg
     1021 cgaatgtaaa tgctctctta caagtaaatc tattgaggag aactttaagt aaatgataca
     1081 atgaaaaaga aatggaattt acgtgtgccg aaaataaaga atcctatgga tcggtacatt
     1141 ggataataga tagattatag tttggcagaa agcggcgggt gtacaatggt tattttttat
     1201 ttcattctta ttctgataat tatagggatc agttaccatt ttctttctta tttttatgca
     1261 aagaaattag taagagcggg agtacagaga ggatatgact ggtatgaaga cactggtcat
     1321 cgtttgatgg accctgctgc agtcacgtca gtagaaaaaa aacgacaaaa attagaagaa
     1381 gagtgtgaaa gcttttggga gcagggaaca cccaaaacaa tcaaaagtta tgacgggttg
     1441 aaactagctg gtcagatgtt catccatcca aataagcaga ataaatgggt catttgcgta
     1501 catgattacc gaagcacagg gaaaagagac atgtctcata taggtaaaag atatgcagag
     1561 aaaggattca acgttcttgt acctgattta cgtgcgcacg gagaaagtga aggagaaatc
     1621 atcggaatgg gatggctaga tcggctagat ttaattgcct ggatccaact catcttagat
     1681 gaacagccga atgcgtccat tattttacac ggcggatcaa tgggagcttc tacgatcatg
     1741 atggcaagtg gtgaaaaact tccatcagcg gtcaaaggat ttattttaga cagcggatat
     1801 gtttctgttt atgcagaatt ccgatatatg ttaagtaaaa tcactgtctt tcctaaaaaa
     1861 atggtcatga gatatgcgaa tcattatgcc cagaaatacg ccggttactc cttaaagcaa
     1921 gcatcagcta cgagacagct tggcagcaac catctgcctt tattagtcat ccatggtgaa
     1981 agagatcatt ttgtccctac agaagcagcg tacacgattc aaaatgctac ggcaggggat
     2041 aaagcactgc tgcttgttcc agaagcagaa cacttggaag catcgatgaa agatccgaaa
     2101 acctattgga cagtgatttt ttcatttatt gaacaaagaa taacgatgga ttaattttct
     2161 cttctttttt gtaagtaatt cgaggataaa taattgttta tagatctgga gactgctaaa
     2221 ataaggtagg aataaaaaaa tatcagttat ttgaggagtg cggcatatgg aaatcaaaga
     2281 agaaaaaaat cgtcttgctt tattcaatga tgagcaacaa gagattggag aaatgacatg
     2341 gtcagatgcg ggcccagaca tcatgatcat tgaccataca tttgtagatc cagcatatcg
     2401 tggtcaaaag ttagctgaaa agctagtgta tactggagta gaattagcaa gacgagacgg
     2461 gaaaaagatc attcctttat gcccttatgc aaaaaaagag tttgaaaaga aaccagaata
     2521 tcaagacgtc ttacgtcagt cataagaata acagctgaaa gttttgaact ttcagctgtt
     2581 attccattta tatagagttg tcacggactt ttatctatga taaagttaaa gagacgatat
     2641 cttttgggaa ggaggctgcg atatgtataa tttaattttg ggaatcgtga tcgttttatc
     2701 gattgttatg gtcattgcga ttatgatgca gccaagcaag caaaatagtg cagcgagtgc
     2761 atttaccggc ggtgcggatc aactattcgg caaacaaaaa gctcgcggct ttgaagctgt
     2821 catgcagcga tcaactgcag ttatgggagc tatctggatg atcttattat ttgtcctagc
     2881 atatttatct tcgaagtagt tgaataagcc tctcgcaaaa cggcgggcgg ctttttttat
     2941 tgcaaagaga acgcttaaga agaagttatg aaagaaacta tggtaaaatg aggcgaaaga
     3001 ggtgagacga gtgagacaat taccaaaacc cctttatgca aaacatggaa agcgagcggt
     3061 tttgctgctt cacgcttact caggaagtcc aaacgatgtt cgaatgctcg cacgattttt
     3121 ggaaaaagca gattatacag tttatgcgcc gctttttaaa ggacacggaa caatggagcc
     3181 atatgatatc ttgcaagaaa aagcagaaag ctggtgggca gacactaaaa aagccatcca
     3241 ttttttacag tctgagcagt tttctgatat tgccgtatta ggattatcaa tgggcggcat
     3301 ctttgctgta cgagcactag aagaagaatc agtgattggc ggcggttttt tctgttcgcc
     3361 tttgtctcct gtaaaaacaa acgtgcctga aaactttgaa aaatacgtac gtcaagtgct
     3421 gaagacagca ggtaaatcag agaaagaaat caatgaaaaa gcagttgctt accgctcgtt
     3481 agctgaacaa caattaatgg atatacaaga tcaggcagca atcgtagaaa gtaggttgtc
     3541 agatatccaa cagccgatat ttttagctca agctgggaaa gatgaaatga tcgaccctaa
     3601 tggcgtattt gaaactgcac gcaagttgag ccgtcagcga gtgacacttc aatggtatcc
     3661 tgaaagtggt catgtgatta cagttggtac agcaagacgc gaattagaaa aagacgtctt
     3721 ggaattttta gaaaaattac cttggaatga ggaataaata tgacaaaaga aacgataaaa
     3781 gagaaaattc tctttttcat ggaaagcagt cgcaaacgca gtttttctat ggaagaaatc
     3841 gctgaaggtt tagggttcca aaaaagcgat gattttaaat tactagtaca aacgattgct
     3901 caaatggaaa gagaacagag tatagttttc aataaaaaag gaaaagtgaa acttccttta
     3961 aaaccaatct tgatcgaagg aacatttcga gcaaatgaac gaggatttgg atttgtaacg
     4021 attgatcccg aagaagatga catctatatc ccaaaagaag cgactggttt tgcaatggat
     4081 ggagacaccg tagcaatcga tatcatgaag cgagcaaata cagatatgga tcgcggagca
     4141 gaggggaaag tagtcgagat tcgagaccgg gcgactactc agttagcagg ggaattcgtt
     4201 gcttattcag aagaagaaat acaagaaaca gatctttacg gcgttgttat cccgaaagat
     4261 agaaagctca accaatttaa agtatatgca gcagcagagg gagttcgtcc agttgacgga
     4321 agtattgtgc tgatcgaact gacccattac cctgaaaaag ggtatgcgac tagtttagaa
     4381 gggataatca agcaagtgat tggtcataag aatgaccctg gcatggatat cctctcaatt
     4441 gtcgtggcta atggtatccc tacgaaattt cctgatgatg tattgtctga agcagatgaa
     4501 gttccagatc aaatcagcga aaaggatata actggacgta gagatttaag aaatcagctc
     4561 attgttacga ttgatggaga agatgcaaaa gatctagatg atgcagttac ggttaaaaaa
     4621 ttagataacg gtaattattt tcttggagta cacattgcag atgtttctta ctatgtgaca
     4681 caaggcagtc aattggacag agaagcatat gaaagaggga caagtgtata tttgactgat
     4741 cgagtgattc cgatgatccc gcaacgttta tcgaatggta tctgttcact gaatccccat
     4801 gttccaaggc tgaccatgag ttgcgaaatg gagattgatc caaatggaca agtagtctct
     4861 catgatattt tcccaagtgt gatccaaaca acagaacgga tgacctacac agctgtcaat
     4921 caaatcttag aagatcaaga cgaagaaacg atgcaacgct atgctcagct cgtacctatg
     4981 ttccaagaaa tgggtgaatt gcatcgaatc cttgaaacga tgcgaatcaa acgtggcgcg
     5041 atttcttttg aggatcgcga agcgaaaatc cttgttgatg ctgaaggaca cccaaatgac
     5101 atccagctaa gaagcagagg aattggtgaa cgcttgatcg aatctttcat gcttgcggcc
     5161 aatgaaacgg tagctgaaca ttataataaa ttaaatctgc cgtttattta ccgaatccat
     5221 gaacagccaa aagaagaaaa aatgcagcgc ttctttgatt ttgcttcagc tttgggcatt
     5281 ctggtccgag gaacgaaagg aacgatcaca ccaaaagatt tacagcatgt gatcgaagga
     5341 gtagaagaca aaccagaagc tgcagttatc aatactatgt tgctaagaag catgcaacaa
     5401 gcacgctatt cagaagacaa ttttgggcat tatggcttag cggcagatta ctacacacac
     5461 tttacatctc ctattcgacg ctatcccgat ctaatcgtcc atcgtctgat ccgttcttac
     5521 agtgaagata aaagtgaaag caatcaaagt tcgtgggctg cggaacttcc tgaaattgca
     5581 gatcatagtt ctaagatgga acgtcgtgca gtagaaacag aacgtgaagt agattccatg
     5641 aagaaagcag aattcatgat ggacaaagtg ggagaagaat tcgatgggat catcagctct
     5701 gttgtgaaat tcggcctgtt tatcgaactg ccgaatacag tagaaggatt gatccatatc
     5761 aatgaattga aacaggacta cttccatttt gttgaaaatc atttagctct tgtaggtgaa
     5821 agaactggtc tgacttttaa aatcggacaa aaagtgcgcg taaaagtagt caaagctgat
     5881 ccagatgaac gagcagtgga ttttgaactc gtttccgcag aagaggtcac tgcgttggaa
     5941 cgtccaaaag cacgcaatgg aaaatctgga aaacgagaag gaaaacaagc aggcaaacgc
     6001 tcagaaaaaa atggcaagaa gaacaaacga tatagtgata gtaagcaaac gttcggtaag
     6061 aaaaaaggga aaaagccatt ttacaaagat gtcaaaaaga agaaaaagaa aaaataaaat
     6121 gagttggagg aaacagctat gccaaaaggc gagggaaaac taattgcaca aaacaagaaa
     6181 gctcgccatg attattcgat catcgacacg atggaagcag ggatggtctt gcaaggaacg
     6241 gagatcaagt cgatacgaaa cagccggatc aatctgaaag atggatttat tcgcgtccgc
     6301 aacggggaag ctttcttgca taatgttcat atcagtcctt atgaacaagg aaatattttt
     6361 aatcatgatc cgttgcgcac gagaaagtta ttattacaca aaaaacaaat catccggctt
     6421 gaaaatgaat tgaaaaatac tggaatcact gttgttcctt taaaagtcta tattcgtaac
     6481 ggctatgcca aggtattgat tggtctggcg aaagggaaaa aatcttatga taaacgggaa
     6541 gatttgaaac gaaaagatat cgatcgacaa attgatcgaa cattaaaaaa tttctctaga
     6601 taatttcatt atcctttttc gcatattcgg tgcttttgct tttacttaat gataaaattt
     6661 gttagcattt aatctgataa aacatgatta tcataggttt tttcttagat caggagcacc
     6721 gtgtataaag aaaaattcgc ttcgtttttg actgaaataa aaaagtgtct aattttcatg
     6781 aaaatgtttt gaattttctg tttcatggtg gtatgatacc gtgtggttag aaaccgattg
     6841 gaaaaaacca gatacgcaca tgaattgatt ttatgtgaat ttcggggaaa agaggtgaaa
     6901 tggattggat gaacgctcgc taacgttcca cattggacca atttggtttg atggtacagt
     6961 ttgtataatg gtgttactga cctgtttgat tgttttcttc ttagcttact tttttacaag
     7021 aaatcttaag ttgaaaccaa caggcaaaca aaatgcgata gaatgggtca ttgattttag
     7081 tcgaggaatc gtaacagata atctgcctag aaaagaattg agcaatttcc atttgttggc
     7141 atttacattg tttttatttg tttttgttgc aaacaacatc ggtttagtaa caaaaatcgt
     7201 tttacctaac gaaacaacac tttggaaaag cccgacagca gacccgtttg ttactttaac
     7261 tttggcattt ataatgatct tacttactca cttatttggg gtgaaaaagt taggcttcaa
     7321 aggatatttc gtaaattcgt tcttgaaacc atatagtttt atgtttccga tgaaactgat
     7381 tgaagaattt acaaacttat taacacttgc tttacgtctt tatgggaata tttatgcggg
     7441 ggaagttttg ctaaccttga ttgcaaaaat gatgacaagc ttaggctggt tctcattacc
     7501 attagcaatt ccattagaga tggtctggat cgcgttctcc ttgtttattg ggagcatcca
     7561 agcatttgtc tttgtcactt tgtcaatggt ttatatgagc cataagattg aagtagaaga
     7621 ataatactcg gttaaaaaac aaactactat ttatctattt taggaggaaa acaattatga
     7681 attatatcgc agcagcaatc gcaatcatgg gagcagcaat cggagccggt tatggtaacg
     7741 gtcaagttat ctctaaaaca atcgaatcaa tggctcgcca acctgaaatg tctggtcaat
     7801 tccgtacaac aatgtttatc ggggtagctc tagttgaagc tgttcctatc ttaggggtag
     7861 ttatcgcctt gattctagtt tttgccgttt aattgaatca gtattaaggc agaaacaaca
     7921 tcgtttgtta ctgctttttt acatggaaac tagaaatact ccaaagaaag gaaggctcgc
     7981 ctatgctgaa tcaattggca attgcagaag ttggcaatgc gatgttaggt aatatcatcg
     8041 ttgtcagcgg ctcatttttg atactactgg ctctcttgaa gcactttgct tggggaccaa
     8101 tcagcgatat tttgaaaaaa cgtgaagaca agatcgccaa tgatttagat tctgcagaac
     8161 aatctcgcat caactcagcg aaaatggaac aagaacgcga acaacaattg ttagcctctc
     8221 gttctgatgc agctgatatc atcaaaaatg cgaaagaaag tggagaatta agccgccaaa
     8281 atattttgaa ggatgctcaa gaagaagcag ctcgtctaaa aagcaaagcc caagctgata
     8341 tcactgtaga acgtgattca gcgctgaact ctgtaaaaga cgacgttgca gaactctctc
     8401 ttcaaatcgc ggaaaaaatc ttgaacaaag aattatctcc agaaatgcat gaatcattaa
     8461 tcaatcaata cattgaaggt ctaggttctt caaatgaaac tagataaata tacagtagga
     8521 agacgttacg ggaaagcttt atttgaactg gcgatcgatt caaatcaagc agaagaaatc
     8581 tatcaagatc tcgtaagttt acgtcaaatc tatgaacaag tacctggatt agggaatatg
     8641 ttaagtgatg ttcgtctaga acctcatgaa aaacgagaaa tcatggacaa gcttgtcagc
     8701 ggttatgatg ggatcgttaa aaacttttta gaagttgttt atgagtataa ccgaatggat
     8761 gaccttttgt tcatgatcga agaatatgaa cgtcgatatg atgaacatca agggttacta
     8821 ttgggaagtg tcacaacagc agtcccactt tcagacgaac aacgaataca attagaaaag
     8881 aatgttgcaa aaacaatgga ctatcaatca gttgaattaa aacaaatcgt tgattcttct
     8941 attattggtg gcgcgattgt tgaagccaac catcgagtaa tcgacggaag cattcgcacg
     9001 caattagaaa aaatgcgtaa tcaattaaac agatagtaca aagaggtagg tgaatcgaat
     9061 ggccatcaaa gcagaagaaa ttagtgcctt gattaaagaa caaattgaaa attatcaaaa
     9121 cgtgctttca gtcgaagaaa tcggtaccgt aacttatgta ggtgacggga tcgctcgtgc
     9181 acatggttta gaaaacgcga tgagtggtga gttgcttgaa ttttcaaacg gctcatacgg
     9241 aatggcgcaa aacttagagt caaatgatgt aggtatcatt atccttggcg atttcgagtc
     9301 cattcgtgaa ggagataaag taaaacgaac aggtaagatc atggaagttc cagttggaga
     9361 ggccttgatc ggtcgggtag tcaatccgct aggtcaacca atcgacggac taggtgaaat
     9421 cgttactgac aaagctcgtc cagtagaagc gatggcacca ggtgttatgc aacgtaaatc
     9481 tgttaacgaa ccaatgcaaa cagggctaaa agcgatcgac gcccttgtgc caatcggacg
     9541 cggacaacgt gaattagtca tcggtgaccg taaaacaggg aaaacttcta ttgcgatcga
     9601 tacgatcatc aaccaaaaag gtcaagatat gatctgtatc tatgtagcaa tcggacaaaa
     9661 agattctaca gttcgtacac aagttgaaac attgaaaaaa tatggcgcaa tggattacac
     9721 aatcgttgtg aatgccggtg cgtctcaacc agcaccattg ctttatatcg caccatatgc
     9781 tggtactgca atgggtgaag aattcatgta caacggtaaa catgtattga tcatttttga
     9841 tgatctttca aaacaagctg ttgcttatcg tgaactttcc ttgctacttc gccgtccacc
     9901 aggtcgtgaa gcttatcctg gggatgtttt ctacttgcac tcacgtttat tagaacgtgc
     9961 agcaaaatta agtgatgatt taggtggcgg ttcaatgact gccttaccat ttgttgaaac
    10021 acaagctgga gatatctctg cttatatccc aacaaacgtt atttcgatca ctgatggaca
    10081 aatcttctta gaaagcgact tgttctacgc aggtactcgt ccagcggttg atgccggact
    10141 atctgtatct cgtgtagggg gttctgcgca aatcaaagca atgaaaaaag ttgctgggac
    10201 tttacgccta gacttagcaa gttaccgtga attagaagca tttacacaat tcggttctga
    10261 cttggatgca gcaacacaag caaaactaaa tcgtggacgc cgtacagtag aaatcttgaa
    10321 acaaaaattg catgcaccgc tagctgttga aaaacaagta gtgattttgt atgctttgac
    10381 acatggattc ttagatagta tccctgtaga cagtatttta gacttcgaac atgaattatt
    10441 cgaatatctg gataccaatc attcagatat ctttgaaaca atccgtacaa caaaagatct
    10501 tccagaagaa gaagcgttga acagtgcgat tcaggaatat aaagacatgt tcttagctac
    10561 aaaaggaagt aattcctcta cagaagataa attgaaatcc attcaaaatg catagtgagg
    10621 tgagatgaat gggtgcttca ttaaatgaaa tcaagacacg tatcgcttca acgaaaaaaa
    10681 cgagccaaat cacgcgggca atgcagatgg tgtctgcttc taagttgaca aaatctgaag
    10741 cttcttcaca gaagtttcag gtttatgcaa caaaagtaag agaaatcgtt acgcatttga
    10801 ctgctactca gttaaatgat attgcctcag aaaatcctag aggggatatc aattacaaca
    10861 gcatgctgct cagccgtgct gtgaagaaaa ctggttatat cgtcatcact gcagatggcg
    10921 gtttagtcgg tggatataac agctcgatat tgaaacaaac gatgtctatt ttagaagaag
    10981 accaccaatc acccgatgat tacgtgatga tcgcaattgg cggaactggt gcagacttct
    11041 ttaaagcacg cggtatcaat ctagcatatg agctgcgcaa tctatctgat cagccaagct
    11101 ttgatgaagt ccgcaaaatc gtaaatatgg caacaactat gttccaaaac gaagtattcg
    11161 atgaattata cgtatgttat aaccatcata tcaattcgct aactagtcag tttcgtgtag
    11221 aaaaaatgct gccaatctca gacttagatc cagaagaagc aacgactttc gaacaagaat
    11281 atatttttga accttcaaaa gaagaaatct tgtctcagtt attgcctcag tatgctgaaa
    11341 gtttgatcta cggtgcgatc gttgacgcca aaacagcaga acatgctgct ggtatgacag
    11401 caatgaaaac agcaactgat aatgctgcta atatcattga tgagctgacg gtttcttata
    11461 accgtgcaag acaaggggct attacccaag aaattaccga aattgttgcc ggagcttcgg
    11521 cactagaata gtattgagat tggaggaaaa aagatgagtt caggcaagat tgttcaagta
    11581 atcggtcccg ttgtcgacgt ggaattttct ttagaccaat ccttaccaga tatcaacaac
    11641 gcattagttg tttataaaaa tgacgaaaat aaatcaaaag ttgttcttga agcagcgtta
    11701 gaattagggg acggagtgat ccggaccatt gcgatggaat caacagatgg tttacaaaga
    11761 ggaatggaag tcattgatac aggcaaagca atctctgttc ctgtaggtaa agaaacatta
    11821 ggtcgcgtat tcaacgtact aggagatacg atcgatttag aaacaccttt cccagaagat
    11881 gcggaaagaa gcgaaattca taaaaaagca ccagcctttg acgaattaag tacaagtaca
    11941 gaaattttgg aaacagggat caaagttatc gatttgcttg ccccatattt aaaaggtggg
    12001 aaagtcggac tattcggtgg tgccggtgtt ggtaaaaccg tactgatcca agaactgatc
    12061 cataatatcg cccaagaaca tggtggtatt tctgtattta ccggtgtagg tgaacgtact
    12121 cgtgaaggta atgacttgta ttatgaaatg aaagattcag gagttatcga aaaaacagcc
    12181 atggtgttcg gacaaatgaa cgaaccacca ggtgcacgta tgcgtgttgc tttgactggt
    12241 ttgacgattg cggaatattt ccgtgatgta gaaggtcaag atgtactgtt gtttatcgac
    12301 aacattttcc gtttcactca agctggatct gaagtatcag ccttgttagg acggatgcct
    12361 tctgcggttg gttatcaacc aacattggca acagaaatgg gtcaattaca agaacgtatc
    12421 acatctacga aaaaaggttc gatcacatcg atccaagcaa tctatgtgcc agccgatgac
    12481 tacactgacc cggcgccggc aacagcattc gcccatttgg atgcgacaac caacttggaa
    12541 cgtaaattaa cagaacaagg gatttatcca gcggtagatc cattggcttc ttcttcaagt
    12601 gctttagcac ctgaaatcgt gggggaagaa cattataaag tagcaacaga agtccaacac
    12661 gtattgcaac gatatcgtga attacaagat atcattgcta tcttaggtat ggatgaatta
    12721 tccgatcaag aaaaagtgtt agtttctcgt gcccgcagag tccaattctt cttgtcacaa
    12781 aactttaacg tggctgaaca atttactggt cttccaggtt catatgtccc tgtagaagaa
    12841 acagtgaaag gattccgcga aatcttggaa ggcaaatacg atgatcttcc tgaagaagct
    12901 ttccgaagtg ttggccgaat cgaagacgtc gtagaaaaag cgaaaacact aggctactag
    12961 aaaggggtgc cccatggatc aatatttaac agtaaatgtg gtaactcctg ccggtttggt
    13021 ctatgatcac catgcagcta tcgtcgttgc acgtacgaca gctggtgagc ttgggatttt
    13081 gcctaaacat gcaccaatca tcgttccatt gacgattgat gaagttcgtg taaaacgaac
    13141 agattcagat acccacgtcg attggatcgc tgttaatggc ggaatcatgg aagttcgtga
    13201 taatgttgtt tcaatcgttg ctgatagtgc ggaacgtgaa cgtgatatcg atgtatctcg
    13261 tgcggaacgt gcaaaacaac gcgcagaacg ccaaatcgaa gaagcaaaag caaaacaaga
    13321 tacgaacgaa ttgaaacggg caactgttgc gttgcaccga gcaatcaatc gtatcaaagt
    13381 atctaagcac tcataaaatg agatgcggat gtttatccgt gtttaagaac agtaaaaaga
    13441 ggttatgaca gaagtggtca gcttcaagac ataagaagga atttacgaaa attgcttctc
    13501 aaatttttgt gaaatctcag cttatttttc gaagaagcta cttctggaac accgtatatc
    13561 agaggttgta aaatcagcgt tttgtcctga gtattagaga aacaaatgga aaatagttcc
    13621 tcatattttg ccgttttttg agctaatacc gagggtcaga tgattgaaca ccgtttattt
    13681 gggctctttg tcaaatgatg ttggtaaaga gaaatcttag aata
//
LOCUS       DAZKCY010000068        13639 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000068 DAZKCY010000000
VERSION     DAZKCY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.069"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..171)
                     /locus_tag="ACG8XC_001827"
     CDS             complement(<1..171)
                     /locus_tag="ACG8XC_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP9191521.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFL"
     gene            complement(650..1369)
                     /locus_tag="ACG8XC_001828"
     CDS             complement(650..1369)
                     /locus_tag="ACG8XC_001828"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP9191522.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1418..3064)
                     /locus_tag="ACG8XC_001829"
     CDS             complement(1418..3064)
                     /locus_tag="ACG8XC_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9191523.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3243..3833
                     /locus_tag="ACG8XC_001830"
     CDS             3243..3833
                     /locus_tag="ACG8XC_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9191524.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3925..5430
                     /locus_tag="ACG8XC_001831"
     CDS             3925..5430
                     /locus_tag="ACG8XC_001831"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFP9191525.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5514..6866)
                     /gene="celB"
                     /locus_tag="ACG8XC_001832"
     CDS             complement(5514..6866)
                     /gene="celB"
                     /locus_tag="ACG8XC_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP9191526.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6866..7384)
                     /locus_tag="ACG8XC_001833"
     CDS             complement(6866..7384)
                     /locus_tag="ACG8XC_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191527.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7400..7726)
                     /locus_tag="ACG8XC_001834"
     CDS             complement(7400..7726)
                     /locus_tag="ACG8XC_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFP9191528.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7739..9718)
                     /locus_tag="ACG8XC_001835"
     CDS             complement(7739..9718)
                     /locus_tag="ACG8XC_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9191529.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9739..10053)
                     /locus_tag="ACG8XC_001836"
     CDS             complement(9739..10053)
                     /locus_tag="ACG8XC_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFP9191530.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10221..10514
                     /locus_tag="ACG8XC_001837"
     CDS             10221..10514
                     /locus_tag="ACG8XC_001837"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFP9191531.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10592..11704)
                     /locus_tag="ACG8XC_001838"
     CDS             complement(10592..11704)
                     /locus_tag="ACG8XC_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9191532.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11857..12705
                     /locus_tag="ACG8XC_001839"
                     /pseudo
     CDS             11857..12705
                     /locus_tag="ACG8XC_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13058..>13639)
                     /locus_tag="ACG8XC_001840"
     CDS             complement(13058..>13639)
                     /locus_tag="ACG8XC_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9191533.1"
                     /translation="IDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAY
                     QLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHL
                     PKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4286 a         2762 c         2330 g         4261 t
ORIGIN      
        1 caagaaccca tctgctgaaa tatgaaaatt tttgaatgct acgacattcg ccattacagg
       61 tatgtaaaag aaaaaaatca tccaaagcat acccggtaca gccatcagga caagtgcttt
      121 ataacgccat agattcataa aaaaatgatt gaaccccttt cgttttttca tctaaaaacc
      181 cctttcgatt cattcgcaac gtaattatag cgctttcaat aatgagaata aataaacaaa
      241 gtatagggaa aatgaaacaa agtataggag aggttgtgaa atcagcgttt tggcctgagt
      301 attagagaaa aaaacgggaa aatagttcct catattttgc cgttttttga gctaataccg
      361 agggtcagct gattgaacac cgtttatcag aggttgtgaa ttcagcgttt tggcctgagt
      421 attagggaga tcgtgtcctt actatctaac tctgagtcac aaggaacaat ggggactgta
      481 acaaaagtct tgtcacagtc ccttattccg aacaaacggt gttccagaag tagcttctac
      541 ggaaataagc cgaaatttca caaaaaagcc atccgaaagt cagacggaca agtctgcttt
      601 tcagatggcg ctacaactac tctgcttatt cttttagcta tccattcaat tatttttctc
      661 tcacttgttc tagctcttct ttggtcatgg gtcgatattg ccctttattc aaatctttag
      721 gcagccaaaa ttttcccata cggattcttt ttaaatataa gacttctttt cctactgctt
      781 tgaccattcg cttaacttgg tgaaactttc cttcatgcaa aatcagacgg ataattgaag
      841 tttctgtctc ttgatctact tgatcaattt ggagttttgc cggcaatgtc tgctcatcgc
      901 catcaataat cagtccttct gcaaataaac ggatatcttc ttctgtcatt ataccgctaa
      961 cctcagctag atattctttc tccacatgtt ttttaggtga tagtaactga tgggcaaaca
     1021 ccccgtcatt cgttaaaatc aagaggccct ctgtgtcttt atctaatcgg ccgactggaa
     1081 aaagatcttc ccgataatcc tggtcttcca ataaatcgat tacggtttga tcatgattat
     1141 cctgagtagc agaaatgact cctgcaggtt tatgcaaaat gtagtaaaaa tctttttgat
     1201 acgtaatgat ttctcctaaa taagtaaccg catcttcgaa ttcatttact tgcgctttat
     1261 cgcttttcac cacttgtcca ttgacttcga tcgttccttt tttgatcaat cctttgactt
     1321 ctttgcggct gccaagtccc atatctgcta aaaacttatc taaacgcatg cgtttccccc
     1381 atttcaaaag agcatcaatc tagctcttct gattcactta ttttattttt aatttatgtc
     1441 gtaaacgcgc tattcctggt cctagtagtc tttctgccaa gcggattttc aaagctaaat
     1501 aaccatacac taaagcccct atacctgcta ctacaagaat caacaataga gactggaatt
     1561 tgctatcttc gtttaaaaaa gcaccaaaaa tcatctttgt gatccacgaa gccaaaagca
     1621 taatcaaggt aagaataaag atcagcaatg tcctgcgcca gacaaaactt ggtttaaagc
     1681 gggctacttc atggattctc tttaagatca aggtacaaga cacagcaaat ccgatcattg
     1741 tcgccaataa agggccgtat acttcaaata aacgaatggc tgggtattga agaaggagtt
     1801 tcactagaaa cccaataacc aaataattaa ttgccgcctt gttttcaaac attccttgca
     1861 gcatgttgga aactagcata tatagtccta ggaagagtcc tacaaaactt gcttggatca
     1921 gtacattact tcctagagaa tccggtgtat aaaataatgt atttagcgga taggcaagta
     1981 acataacacc aaatgtcgcc ggaaacataa aaaatgaaaa caactgtaaa tttccgcttg
     2041 tcaattttgc cagacctact cgatctttca acgtaacagc ttctgtaatc aacggtaacc
     2101 cagtagacgc gatcgatgtt gccaatgcaa taactaccat cgtcaactta tctggattgg
     2161 cactaaaaat cgaaaaaaga tccagtaact gtgcattgga atactcggtg gatgcagaca
     2221 tgatcttcat aaaagtgaac tgatcgacta atttgaagat cgttacccct gaacccacaa
     2281 taataaacgg aatcgcttct ttaacggtat ctaacaacaa ttccttcgtt gcaatattta
     2341 ttttgttttc gctgtagtga ataaacgccg atgtatacgc ttgatgtttc ttcaaaaaat
     2401 acaaaaggac aagaatactt gctagcatac cgataaatgc cgcgaaagtt gattgcgtga
     2461 ccgccgtcac atagtcacca tccatgactt tcatgatgat gaatgtagcc aatagcatat
     2521 aaaaaatacg tgccacctgt tctacaatct gcgacagcgc ataaggcatc atttcttgat
     2581 tgccttgaaa ataccctcgt atcacactca tacaaggaaa aaccaataca gcgatactca
     2641 aagaacgcat cgttggaatc agttcttctc cgccaccaga agcatgagct agccaaggag
     2701 aggcgatgta catgaacaaa gcaaacaaca ggcctaaccc gcccatcatc tgtaatgcac
     2761 ggataaataa tcgtcgtgaa gtgccatatt cattcaatga attatagcgt gcagtttgtt
     2821 ttgcaatcgc agcaggtatt cctgctgtag agatcagtag aaacaatgca tatatattgt
     2881 atcccatatt gaataatcca ttggcagcct tagcattttc gcccatccaa gcataccagg
     2941 gaatgatata gacagcgcct agcaatcttg aaatgatatt actggcggtc atccaagcag
     3001 agcctcgtgc cattttttct tgcggtgtta attgatctaa tgctggtttt ctttgattat
     3061 ccatcggtca gattccaact ttctacaaaa cattactatc attttaattg gtaaaacaga
     3121 aactggcaat cgattttata taacttttat aagaacaatt gtttttttga attcggtttc
     3181 gtttttttgg taaagtaaag aaatatacag ccttttatgg ctaacattta taggagatat
     3241 caatgacaca agaaataaaa aaactacccg ttatattttt aggtcttttc tttatcacag
     3301 ttggattgaa ttggttcttg ctccctcacg atatagcttc cgccggagtg ggcgccatcg
     3361 gacatttagt tgaaacggcc ttttctatcc aacgctcagt tactgtttgg agcatcaatc
     3421 ttatgatgct gcttctagcc gctgtcctat taggaaaatc agtatttttt aagactgtag
     3481 cgggaagtct gctttttcct gttgttttag agattgttcc aaaagtggaa atgatctcct
     3541 cacatttctt ttcactgatt tcaggaagtc ttttgttcag tcttggggtt tacacattgt
     3601 acacagtcgg ggcctcaaat ggcggtataa cgataccgcc gattattttc cacaaatttt
     3661 ttcggcttcc aatgccgcaa ggacttttac ttaccaatag tcttatcgtc tttttgaatt
     3721 attatgtttt tggattgttg gagacactat tcgttctcct atctattaca gtcagttcat
     3781 tgtttatgaa aatactcatc cgcttgtccc ctgtgtcaag aaaatttgaa taaagctgga
     3841 ttttgtcata gcggaaacaa acgatatatt aaaaagagtg tgctataata aaccaagaaa
     3901 ttgaataaag cgagggatca atcaatgagc ttctctttag aagacatccg tcatttacta
     3961 ttaaaagaaa atctattaaa agaatttgtt tcaacaagag gctggcatct tgatgtacca
     4021 gaaaaagctg aatttcttca gttaagttat gattcaagaa aagctgatgc ttctactttg
     4081 tttttctgta aaggaatgaa tttcaaagaa gaatatttgg attcagcaat cgaacagggt
     4141 attcaatatt atgtatcaga acaaccatac gaaaattcag cagtaggaat catcgtaacg
     4201 gatatccgta aagcaatggc gcttcttgct atggcttttt acgactatcc gcaaaataaa
     4261 ttgaaagtga tcggattcac tggaacaaaa ggtaaaacaa ctgctgcgta ctttacaaaa
     4321 gcaatcttgg atcacacaac aaataaaaaa acagcattgc tttctactat gaatacaacg
     4381 ttagatggca aaacatattt caaatcccat ttgacaacgc ctgagtcatt agatttatat
     4441 cgtatgatgt cagaagcagt tgaaaatggt atgactcatc tagtgatgga agtttcttca
     4501 caagcttaca aaacacaacg cgtatacggc ttgactttag atgtcggaat cttcttgaat
     4561 atctcgcctg accatatcag tccgatcgaa catccgacat ttgatgacta tttctattgc
     4621 aagcgacaat tgatcttgaa ttcaaaaaca gttgttttaa atcatgaaag tgactatttt
     4681 gatttactta gggaaacagc cgatctattc cagattccaa cgatcactta tggtcgttcg
     4741 gaagaaagca attatcaagt gatccgttca gataaaggta ctcacggttt tactttatct
     4801 tctcatgaag atcgcttagc tatctgcgat actgcttatg atattttgct ggctggagga
     4861 ttcaaccaag aaaatgcagc cagtgcgatc attgccgcag cactaacagg tgcctccagt
     4921 caagatgcac aagacggatt aaaagaagct cgtgtaccag gccgaatgga tcaattgatc
     4981 caatccaatg gagcgcatgt ctatgtggac tatgcacata attatctaag cttgaaaaca
     5041 ttgctggaat ttgccaaaaa tgaacatcca gatggccgtg taattgttgt attaggcagc
     5101 ccaggaaaca aagcaatctc ccgtcgacat gattttggaa aagtgctatc agaaacagct
     5161 gacatcgcct ttttgacggc agatgaccca gcatttgaag atccgaaaaa aatcgcggaa
     5221 gaaatcaatg aagcaattac taatccagat ttgatcgttc attatgaaat ggatcgtcct
     5281 gaagcgattc gacgcgcttt agcagaaagc gctccgcaag attcagtcgt tattgcggga
     5341 aaaggcgtgg atccctatca aaaaatcaat ggtgtagatg aaccttatga aggggattat
     5401 gcaatcgtga agcggttgat tgaagaataa tggtatttat atactagtgg tttcccattt
     5461 ctaagaaaaa aagggactgt gattttttgt cacagttcct tttttcttag aaatcatttt
     5521 aacttttcac tagctaattc tgctgccttt tctctttctt catttaataa tttattatca
     5581 tagaatttga taaatgggaa ccagataatg aatgcgatga ttgcacaaat gactgctaac
     5641 acagcagctt tccaatcacc accactacca acaaatgctc cgatcccgac tggtgaaggc
     5701 cacggcatct gtgcaagcat cggtttgacg atttccagcc gaatggcaaa ataagcgagt
     5761 gtagcagaag ccatcggagc taagaaaaac ggaaccgcta aatagggatt gtagacgatt
     5821 ggcatcccga aaatgattgg ttcattgata ttgaataatc ctggtacaag tgaggctttt
     5881 cctaacactt tcagctgatc agatttcgcg aggaatgcaa taaagatgat caatcctaaa
     5941 gtcgcacctg atccaccaac ggttacataa gaattgttga attctcctgc caacggataa
     6001 tttgcgcctt gtgcgttagc tgccatatta gctaatacga tcggggtcaa gaaagaagtg
     6061 atgatattcg ctccatggat ccctacgatc cacaatgcat ggatcaagaa ataaatcacc
     6121 atgacaccaa gccatgtatt cgtcaactgc gtaacaaacc caaatgggat agcaatcatt
     6181 ttgaagatat ccgttcctag tgcaactaat aatccattga tgatcatcac cacaaacgca
     6241 atgacaaatg ttgggatcag agcagtgaaa gaacgagaaa ctcccggcgg aacaacatct
     6301 ggcattttta tgacccaatt tctcttgaca cacatacaat acaattcgac cgcaataatc
     6361 gacataataa tagctgtaaa gattcctgaa gttcctaaac gttcgacaaa agagcccatc
     6421 ctaataccat ttactacagt ttctgtctca ttgatagaag caatcaagct gattttacca
     6481 tcagtcatga tcaattcagg gatacacata aagaatgcaa aaacagatag taatgccccc
     6541 gtgagcggat taacatttaa tttttgttca tctcgttcaa ttgacgttaa ttcataagca
     6601 aacacaatgg caaagtaaat agataaaatc cccatcgtca ttgtattgac gatcatgtac
     6661 aagtcactaa ctttgaaaaa agttgcatta aaaaatcctt ctaaaaatgg gaacggtaat
     6721 ggcaaaatat ttaaaaccaa aaacatcgag cctacaatcg tgaaaggaat agaagccatc
     6781 cccgctgcca tgacagcccg aacgatttta agttgcgcaa ttttccccat aggtcccatc
     6841 aggtatttct gcaaaaaatc aaacattagt tattctcctt tcctaaatat aacgcttttt
     6901 catattgttt aatacggata tactgttggt cttggttgag tgaaattttc tttaaacgat
     6961 actgtataaa tgcactggca aaaataccgc ttacaacaag aatgaaaaca aataactgtc
     7021 ctccattgtt atttgccata aaaggataca agaatgtaaa tcctgaggta aacaacactg
     7081 gaatcactaa taaattaaag acactctgga acaaaaaata ccactttgtc catggaacgt
     7141 tcgtttggtg ttttcgataa agctttactt gttctcctac tgcaggcaaa atagctaaaa
     7201 tcagaatcat cggtaaacta cttcctaaac ttttagctga tagaagcaga atcatccatt
     7261 gcatgttcat gaaaaagaaa aaggcagtca ggtaacgtac gattagataa cgattgaaat
     7321 acatgttttt caaactaagt tgcttttttt ctaattcgat tttccttttt actgtatcct
     7381 gcacaatgat ccttcctttc tatttatttt tttgatacaa ataattcatt tcctgtgcaa
     7441 cttccaataa tgtcatcgtc gtcatcagat gatcttgtgc atggaccatg ataatttcca
     7501 tttctatttt tgtcccgctg gcatattctt tcagaagttg agtttgtgac tgatgcgcat
     7561 gaagaatctc ttcatttgct tgtcgcaact tctcttctac ttcttcaaac atctctttac
     7621 gcatcgagcg aaatgcttca tgtatcagtg tccgtgcatt tccgctatgg aaaatgatat
     7681 caaaagaagt cacttgtagt gtttcactgt ttattttttc agccatctgt tttcctcctt
     7741 attcgatcag tttgataaat ttactgctga aattctcaaa aataggttca gctaagattt
     7801 cctgctgtac ttttagctgg tcaactaacc taacgattgc tttagtcacg accgtaattc
     7861 cttcattttc gacataagaa ggcgaaacta aaaaaacaaa atgaatttct tgatattgat
     7921 cccactgcat cccagaagga atcaatgcaa cggctacctt tgtttcgatt ccaactggaa
     7981 cagcgggatg tgggactgcc actgtatcac tgaatatgat ttgtcccata ttttcacgtt
     8041 gctggatctg ctttcgcatt tctcttatat atccttcttc ttcatttatg ctcagctttt
     8101 gcagcaaatc atgaagaatt tcttcctttt tcggttcgta cgtataagtc ttgaaaaatt
     8161 tttcagacaa ctgttcggtg taaatctgct ttttttgctg tagagaaaat gctggtatcg
     8221 gtctatctga ggaatgatag ggtatagatt ctccaactac ctttcgtatt ttacgaacat
     8281 catcttcatt caaaaaaata ctgacatgta aaactggaat tttgaaaaac attgtcgaaa
     8341 gatcaataga agaaataatc aggtcgattc cttttaaagt tgtttcattg atctcataat
     8401 agccttttac acttaccact gtaatatttt taccgaattc actgactacg cgattcttta
     8461 atagttgggc actgccgtaa cccgttgcac agataatcaa tgcatgtagt ttatgcgcag
     8521 ctctttcttt ttccaatgcc gccatgagat gcaaagccaa ataggcccat tcatcttcat
     8581 tgatggtata gtttttcaaa gccggcatgt tgctgaaatg cctttttact gcgtcaaaga
     8641 ctttgggatt ttctttcttt atctcctcag tcaatgggtt ctcaatgcga atacctcggc
     8701 tcaatcgaat catcatcggt gacaaatgat cgagtagtcc attgaaaagc tggagatcct
     8761 ctactaatga aaaatgaaat tcctgagaca atcttttcag aactttcgct aattcctttg
     8821 atagttcctg atcattttga ttttctttgt gattcgactt agccatgaga tgtaatgtca
     8881 aataagcaat ttcttcttcg ggaaacgata cacctgcgat tacctcaata cgttgaacga
     8941 tccgctgcgc cacttgatac tctactcgct tagaaacttc ttgttcgatg cctaattcct
     9001 tgatttcaaa cccttctcgg agccgtttga tccccaaagc caagtgtaaa gtcagattct
     9061 gtatgataaa atccgaaact tttaacttag cttctcttgt ttcatcgaga atgataatga
     9121 ctagctcttc aaaactgatg tctgggaaaa aacggctttg ccctaaatat tctttcaatg
     9181 aatttccata atggtttcca aaaaaagtat ccatgataaa atgccgtcga tttctttctt
     9241 ccccttcgat ccatgtccct tgaccatgct tagaaacgat ttttagtgaa tatggaatca
     9301 atcgctcctt gatttcttgc atgtcctttg tcaaactgga acgactgata taaagctctt
     9361 ctgccaaatc atccataaaa agaatggcat cttccaaaag caattggttc aatatatact
     9421 tttgtcgatc ttcggactca taaaatcctt gcggttctct agcatatcca ggtactattt
     9481 cttgccgact caaaaacaag tcaaatgtca gtctttgtaa aatatgcagt tggtagccat
     9541 aacctggttt agcaatgatc ttacctccgt ttttttctac taactctttg atccgctgca
     9601 agtaattacg gattgtccga tcagataata acaattcaga agctaaacgt tgactggtga
     9661 catactcctt cttgtgatca atcaaatatc tcaacagcaa tttttccttc tgtttcatta
     9721 tcatccactc ctattccatc atatgttctc ttcgatcacc tctgctaact tctggatgcc
     9781 tgtagggatt gggacatacg cttggaaagg gatactaaca acaggctttc caacagattc
     9841 tcctaatttt ttgaatttat ccaaaaacat cgtcgtttgc gggctgatca gaaataaatc
     9901 aaaatcacta ttcttaatca tattatcgcc ttctgtagct gaaactgcat ctacgagtac
     9961 ctcttctctt ttttgcttaa agaaatccgt tgtctttgag gccatcatcg aggatgacat
    10021 tcctgccgca caaataatca atgctttttt cataaatcaa tcactccttt tatttttata
    10081 aaccaagcat atcttgtaag cgttttctat tcaaaatata tatttccgta gcattgcgga
    10141 aaaaatgtgt atcatatctt gaataataat ttctggcacc gtgatatact agtgatagaa
    10201 ttttttcagg aggggattta gtgagtatca cagtgaatat tttgtataca agtacaaatg
    10261 gacaagccag aaaatttgca gaagaaatgg tcgaaagaaa cattgtcaaa gcgattcgtg
    10321 cagaagaagg aaacgagaaa tatgactatt actttccact agaagatcca gaatctcttt
    10381 tactgatcga ccgttggaaa gatgaagaag caatcgaaaa gcatcacaaa tccgaaatga
    10441 tggctcaaat aaacgaactc cgtaaaaagt ataaattaaa aatgaaagtc gaacgtttca
    10501 ctgatttacc gtaaaaagat acacatggaa tgatagcaat ttatcatcca tgtgtatctt
    10561 cactttttcc ttatttttta ttcgatctca tctaatgatt caaaccgtgt ttttctctta
    10621 tttttcggat aagtatttca gtcaccccaa tatacatgat tcctaaacat accccaagga
    10681 tattctcaaa aatgcggatc gttactgctc ctggaacccc gaataaactg gcagtgattg
    10741 ccaaagcacc aaaagaatta aaaactgttt tatatctata ggtagaacaa acacctaaag
    10801 ctagaccacc taatataccg acccagttaa aaggaataaa ttggctgata ccaataaata
    10861 aaacacatcc aatcaatgag ccaatgattc ggtctactgc tcgttctttg aaaccaatcg
    10921 acatcaatcc atagctagat acaatagaag aaaatgcaaa ccctgcccac atcaatcgct
    10981 gaaaagggac atactctcct gcaacgatca aaagactaat gccaaatgca tagatgagca
    11041 tccaaatttt ttcctgcgaa tacatgcctt tcaaaaaaat ctctttaaac aagcttttgt
    11101 ctttgttctt ttctcgatgt tttctgtaaa ggagaactga aaaccaacaa aaaaacaaaa
    11161 ataataagct acttgtttgt gtaaagaaat tcttgttcaa cgaatcttta ggtaacgaat
    11221 aaacaataaa gagatatgaa aagccgtaca aagaagcatt ccccattttg ggatcactag
    11281 cggttgccat taatatgctg cttagtaaaa taaaatgaat caacatgccc ataactgacc
    11341 atttgatcag ctggacatac ggtgctacta atagtgagaa catgacgatt gctaaaccta
    11401 tgatcgaatg ggaaatctta taaccaaagt caacaaaacg aatactcatc aacatacaga
    11461 ataaaaccac agcgtatggt ttatgcttcg attcaaataa tgttgtaatt ataatgataa
    11521 aacaaatggc aaacaatacg atgaataatg atcgtaaaaa caatgaacaa atatacatgc
    11581 acttttcttt tttagaagct gcttctcgta ttttttgttt taaaataaac ggatctagtt
    11641 gtagagactg ataaaaatcc acctgttgtc gtcctttctt tttactttta ttttttacag
    11701 tcatctatga caggttgtaa atactatgga acatcatatg gcgttgtcat tttcttgtca
    11761 actatttttc ctattcatgt ttttataaaa aaacatatcg ttttcttgta tgattaaatc
    11821 accacaaaat aaacaactga gagaacgata tgctttatga atggttctat caaaaaaatt
    11881 ctgagatcaa tagacaaaaa tttgatgatt acagaggtct cttacgaaac cttacagaag
    11941 aaaaaaacat tgatcgttgc tgcagttctc ttccctgctc catgtgtctg tagaaattgt
    12001 ggttctgctg tggtagatgg aaataggaaa ttaatggtgg tgaaaaacaa gaagaaggaa
    12061 accattgtcc actttgaata atataatcat atgccaatgg ttatacgcct aaaaaagcag
    12121 cgctatacct gtaaaaactg tcgaagccat tggaacgctc aaagttattt tatccgtcca
    12181 aggcactcga tttcaaatca tgtcagacat aaaatcactt ctttactgac tgaaaaagtg
    12241 tcgttatttt ttatttcgaa aagttgttag gtatctttga caaccgttat tcgcacattg
    12301 aaagagttga gaagctattt accaaagtct tccaaaagaa ttctgtcttg agtgttgagg
    12361 gttgtgaatt tcgttctcac gtttccgttg aggataaact gagttttatt tccgcggatg
    12421 gcgagacaga aaaattaatc gagaaaattt acctacacaa aaaattcctc gattaacaaa
    12481 ctatttcctt agctgcacta atcctaaaga aatagaattc ttagttacag acatgaacgc
    12541 cacctacttt cagctcacca aacatgttct accaaatgcg aaattggtga ttgatcgatt
    12601 acttggtttt tccacgcctt tcaaggaggc gtaaccttta ttcacgagtt ggttgaagct
    12661 ttccgagaca aaagacccga cttgtttttc tccttattga caaagagcca ttaaaactag
    12721 agaaaaaatt acaactgatc gctgattatt tgtagaaaga aaatcaaagt attacccaat
    12781 aaacttgagt cattaggcaa aaaacactgc gaaactcaag aatgattttc gcagtgtttt
    12841 tgtatatttt ttattaacta gtattactac aaaggtttat ttttcacttc tcaacgcagc
    12901 acaaaattgt ctttacttat ttactggttg ggttcaacct tctctactcc gatactaata
    12961 tttgtttgta taccccttct aaaatcggta gtttccattc aattgatggg tactagttga
    13021 ttttaaaaac tatgcttatt caatttttta ttttagctca tatatttcgt ttctttctaa
    13081 attttatact gatgaaaaca agtagcacac ccaatattga caatataaac tggctaatga
    13141 tggtttctcc agtttttgga agatgcttat ttccactgct tgttggattg tttcctctta
    13201 tatctgagct atcagcaact tttctattct cttttgttaa aaagatcgcc acttcttgtt
    13261 ctttttcgat cttgaaagtt actggtgtcg catccaattc ataccccgtt ggcgcttgag
    13321 tttctaccag ctgatatgct cctggtgcta agccatctat cgccagtttt ccatctgctc
    13381 ctgtggttag acctgactgc agaacggttc cattggcatc ttgtaactca aaaacagctc
    13441 cttgcaggac ttctccgctt tggtcatcga tctttgtcaa cacgacaccg cctggtgtaa
    13501 gtcgattttc tttcgtcaat tctaccaccg ctgtctgact tctttcgatc tcaaactcga
    13561 ttggtgtcgc atccaattca taccccgttg gcgcttgggt ttctaccagc tgatacactc
    13621 ctggtgctaa gccgtcaat
//
LOCUS       DAZKCY010000069        12884 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000069 DAZKCY010000000
VERSION     DAZKCY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.070"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..1386
                     /locus_tag="ACG8XC_001841"
     CDS             52..1386
                     /locus_tag="ACG8XC_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9191534.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            1427..2338
                     /locus_tag="ACG8XC_001842"
     CDS             1427..2338
                     /locus_tag="ACG8XC_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9191535.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            2335..3189
                     /locus_tag="ACG8XC_001843"
     CDS             2335..3189
                     /locus_tag="ACG8XC_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9191536.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            3186..3803
                     /locus_tag="ACG8XC_001844"
     CDS             3186..3803
                     /locus_tag="ACG8XC_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFP9191537.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            3805..4323
                     /locus_tag="ACG8XC_001845"
     CDS             3805..4323
                     /locus_tag="ACG8XC_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFP9191538.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            4620..8889
                     /locus_tag="ACG8XC_001846"
                     /pseudo
     CDS             4620..8889
                     /locus_tag="ACG8XC_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
     gene            8889..10202
                     /locus_tag="ACG8XC_001847"
     CDS             8889..10202
                     /locus_tag="ACG8XC_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP9191539.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            10195..11892
                     /locus_tag="ACG8XC_001848"
     CDS             10195..11892
                     /locus_tag="ACG8XC_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9191540.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            11966..12859
                     /locus_tag="ACG8XC_001849"
     CDS             11966..12859
                     /locus_tag="ACG8XC_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9191541.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
BASE COUNT         4240 a         2197 c         2840 g         3607 t
ORIGIN      
        1 tatcgttata ttaaaaccgg catcggttac attaaaattt ggaggtaaaa tatgaaaaaa
       61 ttattgttta cagggttgct ggcaagttca gcattgtttt tattaggagg atgcggatca
      121 agtaatgcaa gcgatgccag ttcaagtgca gcaaattctt ctgaaaaaac aagtgatgga
      181 agaaaaaata ttgatttctg gtccttttgg ggatccggtg cacgaaaaga agctatcgaa
      241 gaaatcattg aggatttcaa tcaatcacaa aacgagatcc atgtaaatta taagtatcag
      301 ccatggggtg acatctggac taaatcttta tctgcaataa cagctggaaa tgcacccgat
      361 gttattgtac aggacataaa ttccgttgcc caaagagcag aagcacagca agcaactaat
      421 ctctccaaat atgtagaaaa agacacttct aaggattttt atccgcagct ttgggataca
      481 gtagaatatg aaggtgatcc ttatgcgatc ccattcaata cagatacaca agtcattttt
      541 tataataaaa agttatttaa agaagctgga atcagtgaac aagatctacc gacaacttgg
      601 gatgaattag aagaaacagc acgtaaacta gataaaaaag aaggaaaaga atttacacgc
      661 atcggttttt atccattatg gaatttaggg gctgatgtat gggcgctaaa cgcagataac
      721 gggactagtt ggtttgacaa agatgaaaaa gtcaagattg atactaaaaa taaagtggaa
      781 gccttggagt ggatccaaga ctggcaagat tattatggga aaaatacgat caataaatta
      841 gaagccgaat ttgggtctgg tgtatctgat ccatttattt ccggattagt agccatgcgt
      901 gcccaaaata tcaattatta ctctagtttg atggaaaatg caccggatga ttttgatttt
      961 ggcgtgatgc agctcccaga aaaagaagaa ggttcaggcc attggtcgtg gggtggcgga
     1021 ttcgtcttgg aagtaccata cggagcaaaa gatcctgagg cctcttatga attcatcaaa
     1081 tatctttctt ctccagaagt acaagaaaaa ttcggcgaaa aaagttttga tatcatggcg
     1141 agcaagacag ccaatgagaa tttagtgaat aatgacaacc taacggataa aggaaaaatg
     1201 atctatcaaa tggcggacga aaactttaaa aatacggtga tcacaccagt tcctttagat
     1261 gctccggatt acacgacatt agtgaatgaa cagattgacc agattctttt aggaacaaaa
     1321 tcaccaaaag aaggattggc agatgcacaa aaagcagtag aaaacttagt caagcaaaat
     1381 caataatcca tttttgactg tctaggtaca gaggaggcaa gaaaatttga agcgtttatc
     1441 tatccgaaag aaaaaagaag caagatgggc ctatttattt atctcgcctt ttattatcgg
     1501 gttcttggta tttatgctag ggcctatgct tttttcagtt attggtagtt tcaccgatta
     1561 taatttgaca tccaagatga atttcattgg tctagcaaat ttcaaaagaa tgttcgttca
     1621 cgatgattta ttttggaaat cgttatacaa caccgtttat ttcgtcatct tcaatgtacc
     1681 gctgacgacg attttcagtg tgtttttagc cacagtactc aatcaaaaaa tcaaaggaat
     1741 cagtgtattc cgtacagctt tctatttacc ggcagtctta tcaggagtag cagtctacat
     1801 cttatggatg cagctgcttt ctccatctgc aggattgatc aacacatttc tgggctggtt
     1861 ccatatccct ggaccagctt ggctgtttga tccacaatgg acgaaacctg cgttgatttt
     1921 gatgaaaata tggagttctg gaggagcaat gctactttac ttagctacat tacaaaatgt
     1981 tcctagatct ttatatgaat cagcagaaat cgatggagca ggcgtttggg gaaagttcaa
     2041 aagtattact ttgccattaa tcactcctat tattttttac gatgtgatca cctctacgat
     2101 cggttctttc caaatttttc aagaagctta tgtcatgacg aaaaatggaa caggcggacc
     2161 tgcaaattct ttgctttttt ataatctgca tatgtggaac aaggcgttcg tagcttttga
     2221 tatgggttat gcgatggcta tgtcaatgat cctgtttctg atcgtgttgg ttctgacgtt
     2281 tgtcaatttg aagcttgcac ctaagtgggt ctattattct ggaggtgacg gcaaatgatg
     2341 gattatttca aaaagaccag tgtgaatcgg caaacaaaaa aaatactaag ttatgtcctc
     2401 atgacagtaa taggaatcat tttgatcatt ccattgcttt ggatggtgtt tacttcattg
     2461 aaaccaatgg aagaaatcgt tcgttatcct ccgacctttt tcccagaaaa aatcgtatgg
     2521 gaaaactatc tggatacgat tgccgccttt cctttttggc gctatgcaag aaatacatta
     2581 ttcatcactg tgctagtagt gatcggtaat gtcctttcaa attcttttat tgcttatgga
     2641 tttgcgaagc ttgattttcc tgggaaaaag ctgatgtttg ctttggttct ttctacgatg
     2701 atgatcccgg ggtttgtaac gatgatccct caatatgtat tattctccaa gatcggctgg
     2761 gtaggtactt atttgcccct gatcgttcca tcgttcttcg gcaatgcgtt caatatattt
     2821 ctgatgcgtc aattttactt atcgatcaat aatgaattga tcgaagctgc tgaaatcgac
     2881 ggagcgaatc atttgtatat ttggagtcat ttgatgcttc cattgacgaa acctgccttg
     2941 attaccattg cgatcaattc cttcaacgca gcttggaatg atttcttagg tcctctgtta
     3001 tatatccaag atcaagaaaa atacacgtta cagattggct tgcaagtttt tcagaaccaa
     3061 gcaacgacac aatggaatta tctgatggca ggagcaacac ttgtcttgat cccgacgatt
     3121 ttgctgttct tttttgcaca acgttatttc attgaaggga tggatttgac aggaggttca
     3181 aaaggatgat gaatcgatcg attggtaaaa aagccaatga ttatttctta gaaatcattg
     3241 aatggattcc acgtcttatc catttacagc tactatggtt cctgtgcgtc ttgcctgttt
     3301 tcacattagg aactgcaagt cgaacagttc tgtatatgat ttatcattac aaaaaaaatg
     3361 aagagaaaaa gctcggttca ctattctgga aaaagtttcg tgagaacttc tctctttatg
     3421 gaaagcaaga tagtttagca agtgtttatt ttcttcttct attgatcgat tatcagattt
     3481 tccgctattg gggtggggga attggctata cattgatgta tactacgttg tttttgcttc
     3541 tattaagcgc gcttctcttt agttacaaaa tcttgcttca actggaacaa aaagaagctt
     3601 cttggattac tgcatttttc ttgtttttta atgatttcaa aagcgcgctt ttctatttgg
     3661 caggcacgtt gatccttctt gtcgcattct ggtttgcagg accaatctat tttgctttat
     3721 taggtttcag tttcttgttt tattttcaag tcctgctttt tatccatcgt acgcaagaaa
     3781 aggttcttaa aaaggaggaa taacatgttg cgtttgattg ctaaaatcga tgatagtatt
     3841 atcaacaagt tcgccaagca acggaaaacg gatagtcgaa aaatcaattt tttgaaattt
     3901 atcagtaata tctctaatgg tggttttatt tggttgctcc ttgctgctgt tttagtcttg
     3961 atacctgata ctagagaata tggtctagcc attcttatcg gcagtttgat ctattttttt
     4021 gttggtttgc tttttatcaa aaaaacgaca agacggaaaa gaccttatga aaaaaatcgt
     4081 cggcttgtcc tttacacaag taaaccgaag acttcttctt ttccttcagg acatacagcg
     4141 ctagcctttt tatcagtagc tgcgttgttc gaattaagtc cagttgcagg aatattagcc
     4201 tttttatttg cttgtttgat cgcttattcg aggatttttt tacttttaca ttatcctact
     4261 gacattttag gaggagctat tttaggaagt gttattggca tcctatgcgc tgccgttatc
     4321 tgaattaatt gtacgaataa agcatgagtg cgtaaatgcg catttgtgct ttattttttt
     4381 gtttcgagga aagcgtagtt gtttattcat ttgtcttaat aacttaagtt ttgttaaatg
     4441 agaacgttat aaaattttat aatttaattt taataataaa tgttgtataa ttggtgtttt
     4501 taactaaaaa ggatatcatt tgatttaaaa ttaaaattaa attaaattat aattagatgg
     4561 gcattgtttt gttgattgtt gtgaaagaag acaaatattt ttgaaaggag tataaggatg
     4621 tgaaagggtt aaaattgaag cttttgcagt tagtaactgt cagtttgcta tttggaccat
     4681 atttccagtc agtctatcat atgacgaccc tggatgtagc tgctgagtca acagaaacaa
     4741 aggaggatga ggtattctta gatgaggact atgcaaaaat tgaagggaag tatattgaaa
     4801 aagaagaaac ccttgaatgg catctggctt ttgaaaaaaa ggaaagtgcc aatgaaggta
     4861 gaatacgttt agcaatcgat gcggcagccg ccgggattgg taccgtaaaa aacgttcgtg
     4921 gaacaggtct atcctcctat gatgaagatg gagaagacct aaaagtcgag acgatcgatg
     4981 gtcaagaatg gtatgtcgga aaagtttatt caaaagaagc agaaaccggc acattgattt
     5041 ttgaaacaga aaagatcccc gatgtgaacg aagggtaact acctcttcaa gtggtggttg
     5101 atgaaagaat agatgaagtt tacaacgaat gactatacaa aagtgttagg aaattggaca
     5161 caaagcaatg aacgctatca aggagacaaa ggctcgccat atgacggaga gaacttgatc
     5221 gatgatagtg attatacaaa cgggggagcg aactggcgaa attacgacta tgctactaat
     5281 aatggatcaa ctgcaggcga agaagataga aacgttccag ctcaaaccgt tcagctttgg
     5341 ggaaatgaac ggaattttga aaacagttac ttggattata acggggcgta catcaaaaaa
     5401 tgggtagaac ctgttctgcc aagcagcaca gcatctgatc tacatcctga agatgcaaca
     5461 accctttaca atgtctatct agatgtcatc gggggcgaga aaaaggaaat cagtccaatc
     5521 gatatcgttt ttgttctaga taagtcagcg agtatgagtg aattaaccgc tgggaccaat
     5581 agtcaaacta aaaatgctgc gttgattgag gcagtaaacg aaatgagtaa agatctgctc
     5641 tctgacccga gtctggatat tcgaatcggt atggtgaatt tttaccataa tagtacagct
     5701 atcaataacc atgagcaaat cagttcggac atctttccat tgacaaatga tatcaatcgt
     5761 ttgacgggaa gtgagaatac agcattaaat cgtacaccaa tcggaggaac tccgcttaca
     5821 ctagggctta aaaatggcta tgaaacattg tataaagaca atggcggaga aaatagaaat
     5881 ccagaaaaaa tcctaatcgt tgtgggagat gggacaccga ccttcagtta tgcaccgatt
     5941 cagtcaagtt atcgtacgag cactaatgga gcttggagca actggactgt gatggaagat
     6001 aagattgctg aagataatga cgttttgttc agaaattttg aggagttttc tgggaatact
     6061 tctaatgcag ggtttactca tccagttacc tatgcatctg actttaaccg tccagaagat
     6121 gaagtaaatg tacactatcg ttatggagag gtaaaagaag gagataataa agcgactcac
     6181 tgggttggtg atggttcttc taataacaat acgaatggca gtcctacttc gcaagaaaag
     6241 tcatctgcga tcaacacagt tgcttatcat cattggctga aaaacaagta tcaagagaac
     6301 ccgccaagta tcttctccat tggtttagga atcgatggaa acgttagcgg gagacaacga
     6361 ctagatgcta ttggacgaaa tgtattgaaa aatattgctg atttagaaga agatggcgta
     6421 acaccgcgat attacaatgc gaacaacaag aatgacattg ttacagcctt ggaggatatc
     6481 tcttctacct tcaagagaac gatccagcaa gccacacttt atgatgaaac tggttttaat
     6541 gtctcaccat ttggagctgg cagatcggca gatattcagt tttaccatct agatccagga
     6601 gaaaacggca tgaagtacca agagccagaa atatgggatg aagaacgtca tggacaagcc
     6661 ccaccagaag tagtggcgac tccgacaacg tatgtcggac gggaaaacca agcttataaa
     6721 ttttctccga tttctttagg tgaaggggaa atggtcagga tcaagtacca agtaaaactt
     6781 gatgcgggag ctcaagacgg aaatttttgc acagtgaaca atatggccta tattcagaac
     6841 ctggaggatt atgagaatga tatcactact ggacggatgt atttcccatc ttcttccatt
     6901 cgttacgaac ataaaggccg taatttccaa gtcagcaaga agggtcaaaa tggcgagccg
     6961 ttggctggag tcgaatttgc attatatgac caaaatccag agagtgggga agccgaacca
     7021 atcgaaacaa gtaaaactac cacggacgga atagcgatct ttgaaacgaa gatgccgatt
     7081 ttaggagatg aaactggagc agtcagcgac atattttgga tcagagagat cgctggacca
     7141 ccacgttatc agttagtaga tgagacgtat caattccaaa tcaaaaaata tgcagccaac
     7201 gaagatgaga tgttaggtca ctacgagctt gtagatgaat atacatctgg ttcgagagtc
     7261 ggcggaccgg aaggtaaaga tccgaattcc tattatgaag gagaatatca caagctttct
     7321 gttgtacaag agcctaacat agaaggcaca gagtatgatg agttgtatgt caaactggaa
     7381 atgcgaaatg aattcaagcc aatccacatg aatatcaata aactaattga aggcagtaat
     7441 atgccaatca atggcgcaga gtttgaactt tatgaaacag atggtaacgg actggaaatc
     7501 ggatatccag tagcgaaagg gctttctggt gtgaacgaag tagaggaaga gaaagggaga
     7561 ctcacgttct attcagtaga tgaagacggg aaatatgtaa tcgtggatgg tgagaaagtt
     7621 attcatccaa tcggagagcc agattcattt gtttataagg atagtgaaga aactaccaac
     7681 tatgcagaat acaaaatcat tgagagccag aacccagaag gtttcaaaga acctgatgaa
     7741 aatgacacat ggatcttgcg actttacgat aatgctatag ggactatcca actcaagcaa
     7801 acaggtgaag aaaattggca atatctcacg tatacaacag atgaagagga tcgtttatgg
     7861 gtagcattca ctgcttacaa tacgtttgat tatcgggaga tccgagttag aaaattagac
     7921 catgaaggcc atacattaga tggtgcaggt ttcgatatca ggaaagtggg ggattcttcc
     7981 ttcccgctgt atcgagcata cactggtaat ccgttagaac cagaagaatc gaaacgtttg
     8041 cctgggatcg gatacttcta ttctcgtgcc gtaggagtag aaaagtatgt cgatttaaat
     8101 tacgaccaac ctctgcatct gtctattggt gaatatgagg tctcggaatc tgtcgcacca
     8161 aatgggtatc ggctatctga tcaagtgttc cagtttgcac tgaatgaaga aggtaagttt
     8221 atcattgaag atacggtcat cgaagacgaa actgctccct tgccggaagg ctatgaaatc
     8281 atcgatggtg tactacaagt aaccttagaa gatgaactag cgccaatcga tttggaactt
     8341 ttgaaaattg acagtatgaa tagccgacgt cttgaaggcg cagaattttc aattgagaaa
     8401 caaaatagtg caggagagta tgaaatcatc gagggaggct tggcaccaga tccaaacgat
     8461 tcttccctat tcaatgcttc agacttgttg gaaggggtct atcggatcaa ggaagtgaag
     8521 tcacctgaca gctatagaaa acttccaggt tactttattt tagaaattag ttatcgagaa
     8581 gaacctgaca tagaaaatga tcgggtagtt ccaggaaaag aagcgggtac actaaaagtt
     8641 gagatttcgt attatccaaa cgaagaagca gagacgcctg atctacagca agagttagaa
     8701 tatgaacttt tggagacaaa tcgaatcaga ttacagctta gtgtcgggaa cgatcctgaa
     8761 aatccgttgc ctgctacagg aggatccgga cgattattct atatcctgct tgctgcattc
     8821 ttcatggcgc ttgcaggagc agcctatggt ttgtttgtca gacaatctaa aaaggaggga
     8881 gcaaggtaat gaaaaagctg ttgtgtttga tgcttcaatt acttgtttta ctaccgcttg
     8941 cagcagtcct tcttccacat gcgatgtcgg cagaagaatc aggggaagat gtcgtggaat
     9001 tcgtgttgca taaacggatg attcgagata tcgattataa cgatcaattt gaataccatg
     9061 aaaatgatgg cttggctatc tctgaggaag caggggaaac agcagatatc atttcacaga
     9121 cggtgccact aaatggagcg acatttgcca tttacgacat gaccgattat tatcatgaaa
     9181 aaaaagctgc tgaaaacatg actcctaaag aattcgtcca gcaaatcgct gaaacgaaca
     9241 gaaacaatat ccgtcagcta atcacagaag aaaggcttgt ccaaataggg acttcggtaa
     9301 caactgggaa agatacggtt ggcggattag gagacgggat tgcacgtatc aaagcaccta
     9361 agaaaaacca agatcgggat gcagtttatt tgattttcga agaatcgatt gatccagaag
     9421 ttggattgga tgttgatatc gatcagacag ctatcccaat tgctgctatt ttgccaattt
     9481 atcacccgac agaatcaacc gaggaactgc aagagattca tatctatcct aaaaatgtcg
     9541 gttatctaag agatccatac ttcttcaagt atggacgtca aagaggaaca actgaaaaag
     9601 gaaaaccatt agaaggtgct gtttttgctc tatatcagat gatagaaggg gggaaatatt
     9661 atttagatat ggctcctgct aatgacttga aaaatcaatg gatcaaacca gcagacaatg
     9721 atcctttgaa tgacaaaaat gtaagcaaat tcatttcgga taaagagggc cttgttacga
     9781 caggacaacg tttcttgcct tctggcacct attattttga agaattaaaa ggtgtggaag
     9841 gttacgagat ggatgaagca agtaagagaa tcgaggtcat catccctgaa ttttgggtgg
     9901 atgcaaaagg ccaaccacaa tatgtagtag ttaacggaca ggagatggct gaattagaat
     9961 ccgggaaagt tccagtctct gcctatgaat ccgcaacacc acgtgtttat aacgagaccg
    10021 taaaagaaac aacggacaaa ccgaaagagc cgagtggtcc aagcaatcca ggtcaatcag
    10081 gaaaatggtc cgtattcccg caaacgaatg aagcaagaac gttgatttct ttactaggaa
    10141 ttcttttatt gttaggtgct gttttattaa taaaaaaaga acggggagaa aaaaatgaat
    10201 aaaaaaatga ttgtcacggg aatgagtgta ctgttactcg ttactgcggg aatagggatt
    10261 ttcgggaacg gaaaactagt caaagccgaa gaagtcgcac aaaaaccaag tgaagtgacg
    10321 attacattac acaaaaaagg tttctcatct gttccggaag aacgaccgaa tagcggattg
    10381 gtttctacgg attttggtga agaaaatatt ccaggagtcg attttgattt gtttgacgta
    10441 acggaagtat attacgattt gattcgagat aatcctttaa caccggaacg agaagacggt
    10501 cttaattctg ccgaagcgat tgaatggatt caaaaacggc acacagaaag ttggttttta
    10561 aaatatcgac tgacaagtat tgacaaacaa acaacgaatg aagctggaga agcggtattc
    10621 tctaccgtcc aagtgacgga agaagcacct tcttctagag ataaagtcta tcttttctta
    10681 gaaacttatt cgccagctca tatttccaga atcgcgtctc caatggttgt tatgatgcca
    10741 gtgatgatgc ctgatatggt ggatggcgtg tgggatggtt ctacatggaa agacacatac
    10801 aatacagatg tgcatttgta tccgaaaaac gaaatcagag aagcagataa acagatgaat
    10861 gtagaggaaa gcgaccttag acaagtaacg attattaatg aagctggaga acaagaaacg
    10921 atttcttata ttgatctaga aagaggaaaa acggcttctt acacgatcac agcacctatt
    10981 ccttatttca tcgattctgt ccttgaaaac ggaagcgctg ttatcaaaaa ttacaaaatc
    11041 acagatacgc ctacagttgg tctaacttat tatgatcagg aaatagaagt gcgagccggc
    11101 gagacgattt taacaaaagg acaagattat atagtagaag tagtaagcaa cgggtttgtc
    11161 gtgacgattt tgacagaaga aaatggagta gccaaagtag atactttagg tagattagca
    11221 gatgcacgtg gaggagattt aacgatcacg tataatttga aggtttccac agaactagaa
    11281 gcggatgatt tccataacaa cacagcggtt attgaaatcg gacgaaatga tgaatttgat
    11341 tatgaagaag gagtagagcc gccagagaaa gtcacaacag gtggaagaaa gtttgaaaaa
    11401 tatgatgcat caagcagtga attgcttaaa gatgcaagat tcgaattgtg gaatgaagac
    11461 cgttctgaat acgcaatatt ttataaaggg gagtcgcctt tagctgttta tgaatcagga
    11521 gcagatagaa ttgagtgggc aacaagcgga caagcaacag aatttgtcgc agatggaaat
    11581 ggatactttg aggttcaagg gttagattat ggaacctatc aaatgaaaga gaccatggcg
    11641 ccagaaggtt atgtacttcc aacaggtgag gcagccttta ctgaatttat catctcttat
    11701 ggaagctata atgaagaaat tcagatcgta ggagtagaaa atccagggcc agaaagagta
    11761 ccgaatatga aacgcgggtc acttcctgct actggtggga acgggctctt ggcattctta
    11821 ctaatcggaa taagtttgat gattggagcg tacagctggt accgtaaatc taaaatgaag
    11881 tctgaagtat aagcaacaaa aataagctgc agcaatcggt tttataaagg ttgctgcagt
    11941 ttttttaaat aagaaggtga agaaaatgaa aaagaaaata ggtaatagac ctcaaaccca
    12001 aatcagtctt ttattacaat tatttatggc agtgatgttt cttgccggag ctgcagtttt
    12061 tacatatcca tttttagcag atgctctcag caattatttg gatcaaagaa ggatcgagaa
    12121 ttatcaaaag cagcttgaac gcgataaaga agaaaaacat gagcaacaat tagcagtgca
    12181 tgaaaagaaa aatcagacgc ttgctcatac atcagtaatc ccaggaatgg gacaagtcaa
    12241 agatcctttt gagcaagcgg ttagaaatgt ccaaaatcca ggaaaagaat attacgaaca
    12301 acatatgatc ggagccatct atattccgaa aatcaatgtt agcttgcctt tatttgatga
    12361 aacgaatgat cttttgttag atagaggagc gacagtcctg caaggcacct cttttccaac
    12421 tggaggagag agtacacatt ccgtgattac cgcccatagc ggtattgctg agaaaaaact
    12481 ctttacggat cttgaagaaa tggagcaaga tgaccgattc tatcttgaag tgtatggtcg
    12541 gatgctcgct tatgaagtcg ttgagaaaat tgttgtcctt ccaacaaaaa caaatacatt
    12601 agcaattaga gaaaagcagg atctagtcac acttatcact tgtacacctt acaccgtgaa
    12661 cactcaccga ttactagtga caggaagacg agttcccttt acagaagaag tctcttcaaa
    12721 gatagaacag acaaaaaaat accatcttta tcgattactt atactccttc taggtatatt
    12781 gctagtgcta acgcttttcg gttattggtg ttatcgaaaa tttaaaagac aaaaacaacg
    12841 aaagaaaaat aacagataat agaccaatta ttaagaataa cata
//
LOCUS       DAZKCY010000070        12815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000070 DAZKCY010000000
VERSION     DAZKCY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.071"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..3661
                     /locus_tag="ACG8XC_001850"
     CDS             134..3661
                     /locus_tag="ACG8XC_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFP9191542.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3658..7380
                     /gene="addA"
                     /locus_tag="ACG8XC_001851"
     CDS             3658..7380
                     /gene="addA"
                     /locus_tag="ACG8XC_001851"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFP9191543.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKRKKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7449..7790
                     /locus_tag="ACG8XC_001852"
     CDS             7449..7790
                     /locus_tag="ACG8XC_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9191544.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8099..9142
                     /gene="pheS"
                     /locus_tag="ACG8XC_001853"
     CDS             8099..9142
                     /gene="pheS"
                     /locus_tag="ACG8XC_001853"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP9191545.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9147..11567
                     /gene="pheT"
                     /locus_tag="ACG8XC_001854"
     CDS             9147..11567
                     /gene="pheT"
                     /locus_tag="ACG8XC_001854"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP9191546.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11737..12747)
                     /gene="trpS"
                     /locus_tag="ACG8XC_001855"
     CDS             complement(11737..12747)
                     /gene="trpS"
                     /locus_tag="ACG8XC_001855"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP9191547.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4334 a         2235 c         2719 g         3527 t
ORIGIN      
        1 ttttttgcgt atacttaaaa aagtagaaat atcagaacct taaaaggata taatttgaag
       61 aatatagaac aaatgttcag tattttttga tataataaag gagcaccgta tcatgtgttg
      121 aaggaggaaa agcatgtctt tacaatttat tcttggaaac gggaaaaaag atcaccgtca
      181 agtcttgatc gacgaagcat ccgcttggct cgaccaggat gcaagtaacc aagtattttt
      241 ccttgttccc aactacagca aattcgaaca ggaacaagaa attttagcgg agatgcgaag
      301 aagaagcgga aaaaaaactt tcagcacaac aaacgttcaa gtatttagtt tttatcgtct
      361 ggcttggtat ttgctgcaac agacaactct tcttacagga aatgaattat cagaatcagg
      421 atctgcaatg atcttgcgac agattttaga aaaaaatgag gaagaattaa caatctttcg
      481 cggagaaatc aataaaagag ggtttattcg tcaattacaa gaactctaca gtgaattgcg
      541 tacaggaaat attcaaccag aagacctgat gcttttattt ggtgattcac ctaatgcaga
      601 agaccagcaa cttaaaatga aagacttgaa gctgattttt tcagcttatg acagcgaatt
      661 gactcaacgt gcactaacaa atgaagatgc actatctata ctagcaaatt atatgcagac
      721 acaagatttc aacaacgtca aatttattgt cagtggtttt tctcgaatca atggtcaaga
      781 gtatcaatta ctgcaggcaa tgatggaaaa ggggcaattg gttgtcgatt tattgctgga
      841 tcgtccttat acagcggatc ttccagaagt acttacgctc tttcatgaga ctggaaagct
      901 ttatttccgt ctttttcaaa gtgcaagaga acggaaagtc cctatattag tggaccgatt
      961 tgcaccagag cagactcgtc cgacagagct tcaactgctg caaacaatgt gggaagagac
     1021 atctagccaa aagaaagcgg cagtacacaa aataggggga caagagcatc tccatttgtg
     1081 ggaagctgaa aatccaactg aagaagtccg acaattagct gttgaaatca gacgactagt
     1141 cagtgaagaa aactaccgtt atcgtgatat ccaagtgctg acaaaaaata tgtctttata
     1201 tggaaatatc cttcggccga tctttcaaga aatggcaatt cctttttata tcgatgaaga
     1261 acaaatgatg gaaaatcatc cgctgatcga atggatcaac agtctgttcg ctcttgaccg
     1321 ctattcttat cggctaagtg atattatgcg ttttttcaaa actgaattat ttctgtcgac
     1381 ggaagcagaa accgaacttg atgaatggaa acaagagcga gatgaattca ggaaaaaact
     1441 ggacatcact gaaaatgtag cacttgctta caattatcaa ggaacttatt ggactcgtga
     1501 aaaagactgg cagtttgtgg cgtatgattt tgaagcagaa cagattgaag acgtacagta
     1561 tttggaagaa cagtcaaata cgattagacg attatttcag aaagctgtac cagtattttt
     1621 gaaaaaaata aagaaaagca aaacaggaca agaggccgca aatgtttttt atcaattttt
     1681 gatccaaagt ggcgtacagc aacaattatt gttttggcgt aatcaggaag tagacagagg
     1741 aaacttagaa gcggcaagaa accatgagca gacatgggga gcgctgattg atctgttgga
     1801 tgaatatgta gtaatttatg gtgaatcggc ttttgactgg tcactgttcc aagaaatcat
     1861 tacaagtggt ctagaaaatc taagttatgg taaaatccct accgcaatcg accaagtgcg
     1921 aatcaatcgt ttggaattag ttcgaccaaa tcaagctgcc gtcacttttg cattagggtt
     1981 gaatgatcaa gtgtttccag accgaaaaga gacaaaagga ttgttgtcca gtgaagaacg
     2041 agaagtatta aatcaggcct tgccagatgg acagttttta ttcgacccat caaaggaaag
     2101 tatctctttt gagcctttcc aagcctatct tgtttttctt tccggaacag atcgattata
     2161 tcttagttat gcacaaagct atgacacaga tggttcgctg aagatgtctc cttatttgcg
     2221 tagaatcaca gaatatttat ctgttcccgt cgagagaaaa gagcatttga cgatcgaaag
     2281 cgaccctaat tgttatgtag ggacttacag aagcggaatc aatacagtca ataggattta
     2341 tcgattagct ttagatgaaa aaaaacctgt accaaagcac tggcaaaagc tcaaagaatt
     2401 agtactgaat tcgtcatggc gaaattttgc tttacagatt tttgaaagtc aggaacattt
     2461 aaatgttcct gtttcgatgc cagaaaaaat ggcggaagag ctttatggaa aagagattta
     2521 tacttcaatt tcacgtatgg agaattttta taattgtgaa tacaaatact tcatgcaatt
     2581 tggtttgaaa ttgaaagaac gaactgttta tggtctaact ccagcagcaa ctggtgattt
     2641 ttatcatgaa tcattggatc gtttcttcaa gctactgttt tcaaaccaat tatccttagt
     2701 gaatatgtcc gatgcgcagc gtagagactt tactgaaaaa gtgctgcagg aagtattcgg
     2761 agaacttcgg tttgagatat tggatagttc tgcaagaatg aactatatcc gctaccagtt
     2821 aggacagacg attcaaaaag tgacatgggc tttgcaaaaa cagagcaaaa atacaggcat
     2881 gcatcctctt cagacagaga ttctatttgg tcagattgct ggagcaaaag gtatcacagg
     2941 tctggaatta ccgttgagta atggtggaaa actccatgtt cgagggaaaa tcgatcgaat
     3001 cgatgtagct tctgaacaag aagatacttg gctaagcgtc gttgattaca aatctagcgg
     3061 acgttcattc gatgtcacag aagcttatta tggcatggct atgcaattat tgacgtatct
     3121 ggacgtagca ttgatggatg cggtacgttt gactggaaag gcggctgtta aaccagcagg
     3181 atcatattat ttacatgttc ataatcctgt attgacggag acagatgatg tagagaagaa
     3241 tacattgaaa aaatttagtt atgatggcgt ttttgtcgat gatcccgctc ttttagaagt
     3301 actagatcat tcactggaag caaaagaaca atcactgatc tatcctgtga aaaaaaatgc
     3361 gaaagatgaa taccaaaaag gtagtttcag taaagacaaa tttttcacgg aagaagaact
     3421 gcaactattc atggcgcata atcgggaaaa tatgcggtta gctggagatg cgattacttc
     3481 cggagaaatc aaactaaatc cagcttataa agataaacag cggattgcct gcgaattttg
     3541 tccattcaga agcatctgta cgtttgatgt gatgctgaaa gaaaataatt accaccgtct
     3601 ggagaaaata gataaggaag aagcactgcg tagaatattg aaaaggagtg aagaagagtg
     3661 aatcaaattc ctttaaaacc gcaaaatgaa cgattcacag atgatcagtg gcaagcgatt
     3721 tttgatcaag gagataatct attagtctct gcgtctgccg gatcaggtaa aactacagtg
     3781 cttgttcgaa gagtgattga aaaactaaaa atgggttttg atatcgatga attattgatt
     3841 gtgacattta ctgaagctgc cgcaagagaa atgaaagaaa gaattcaaga ggcattacaa
     3901 gaatccgtca atagcgaaag tgaccctgta cgtagacagc attttactaa gcagttagtt
     3961 ttattgccca cagcgaatat ctcaacctta catgcttttt gcctgacagt tattcgacgt
     4021 tattattact taattgatat tgatccagtc tttcgcatgt taacagatga aacagaaacg
     4081 atcttgatga aggaggatgt gtgggacgag ctgagagaag cactttatgc agaaaatgat
     4141 gaacgatttt tccaattaac aatgaatttt tctaatgatc gttcagatga cggcttgacg
     4201 aatctggtgt tttctcttta tgagtttgcc cgtgccaacc ctgaccccca aaaatggcta
     4261 gaacagttaa gcgataatta tcgtttgcct gaggggctag cgaaatctcg attatatcaa
     4321 aaacaaatcc gtcctttagt tttggcagat atataccaat gtgtacagtt atatgaacag
     4381 atgactcagt tagcacaagg tgaaggatta gaaaaaatgc atgagcaagt agctggagaa
     4441 caacagcaga tcaagaatat ctatgaagcg ttctcgcaag atcggttaga agaagcttat
     4501 gctggtttgg aacaactaac tttttcaact tttaagagca gtcggaaagc agaattgaaa
     4561 gaaatttcca atgaagtcaa gggcatgagg gataaagcaa aaaaactgat tcagcagata
     4621 agtaaaagtt attttcctgt ttccccaagc caaatggaag aattaacgga caaagcacta
     4681 ccgttagtcg aagaaatgac aaaagtcacg caaagcttca tggacggttt cagtatgcgt
     4741 aaacggaaaa aaggtgtgct ggatttcaat gatctggaac atttagcgtt gcagattctc
     4801 acagaaaaaa cgaaagatgt atggctgcca agtgaagctt caaaacatta tcgaaaaaaa
     4861 ttcaaagaag taatggtaga tgaatatcaa gacgtgaacc aattacaaga agccatttta
     4921 tactggctaa gagaacccga tgatacaaaa ggaaatatgt tcatggttgg ggatgtcaaa
     4981 caatccatct attctttccg tttagcagat cctagtttat ttatcgggaa atacgagaat
     5041 ttctcaaaga aagaaggcgg acgtcgaatc gttttggctg agaacttccg ctctagaaaa
     5101 gaagtccttt cttttacgaa tcttattttt gaacaactaa tggatccagc agtgggtcaa
     5161 atcaattatg acgaagcagc caaactgatc caaggatttt cagactttcc agagaatgaa
     5221 cagtttgagc cggaaatcat gatctatgaa aaagaacagg aagaatcaga gattgagatt
     5281 ccgacggatg atatactgga agacaaaaca gaaggcgaac tttttatgac aggactaaaa
     5341 attcgtcaat taattgattc ctcctttatg atttatgata aaaaatcaaa aaaaagtcgt
     5401 ccgatagagt acaaagacat tgttctcttg acacctacaa agaagaacaa cttaacgatt
     5461 ttagagattt tcaaaacatt ggatattcca ttagagatga atgatgctca aaactatttt
     5521 caagcgacag aaattcgcac gatgatctcg ctgctacaat taatcgataa tccctatcaa
     5581 gatattccgc ttgcggctgt attgcgctca ccgatcgtgg gtctgattga accagaatta
     5641 gccagcattc gattagctaa ccgtgcacat acttactatg atgctgtgct tgcttatcaa
     5701 gcatcaaacg aagatgaatt agcagctaag ctggaacatt ttggcaagca actagagcat
     5761 tggcgagaac ttgctcgccg ttcttcgatc actgacttgt tatgggatat ctattatgag
     5821 acgggatatc tagagtatgt ggtcggtctg ccagcagggg cacagagaca agccaatctt
     5881 tatgcattgg ttgatcgtgc caaagcttac gagcaaagtt ctttcagagg attataccag
     5941 ttcgtgcgtt tcattgaaaa aatgcaagaa aaagataaag acttagcaga accagtcatt
     6001 tcaatagaag ataatgctgt tcgcgttatg acgatccatg caagtaaagg cttggaattc
     6061 ccagttgtat ttttgttaga tatgacaaag gagttcaact tacaagactt gcgcaatcgg
     6121 tatgcttttg aagaaaaact tggagcagga attcgctata tggatcctga aacacgagtg
     6181 ttatatgata cgttaccgtt tcaagccatc aaattggcaa agcaaaacaa attgttgtcg
     6241 gaagaaatgc gtaaattgta tgtaggattg actcgggcag aacaaaagct tttcattgtc
     6301 ggttcctata aaaataaaga gcaaatgatc caaacgtggt ctgaagcagc tgatcatgag
     6361 gagttagttt ttgatcctgc gctacgtttg aagggcagaa gcagcttgat gaattggatt
     6421 ggttacggat tgatccgtca tcctgaaatg cagaaatact tagaggaaga aatttcgact
     6481 tctcttcttc aacattcaaa tgcacagttt tctatttcat ggatgaatca gcaatcgatc
     6541 attgaacaaa gacagttgct tgctgaaaaa gaactggtta acttagatca gcagatgaaa
     6601 gaagatgaaa ctttattagc agattcgtta caaaaacgtc tggcatatga atatccttat
     6661 caagcttctt cacaaaccac aagttatcaa tctgtatctg aaatcaaacg attattcgaa
     6721 gatccagatg atacacaaga atcacgtttg actctggaaa gcagtcagaa caaagcagca
     6781 tcccgtcaat ttcgttatac gcaagaacaa ctagcagaac caaaattttt gcaaaaagat
     6841 cggcaagtgt cagcagctgc tgttggaact gctacgcatg ctttgttaca attactgcca
     6901 ttagagatgc caactacaga gagtatccat caaaaattac aggaattggt aaggaaacgg
     6961 ttagtagacg aaaaagtcgc gaaaaaagta gatgtttctt ctattatatg gttctttcaa
     7021 acagaacttg gtcaacagct gattgccaac aaagaaaacg taaaacgaga acaacctttt
     7081 tccatgctgc ttcctgctga cgaagtattt caagactatc caaatcaaga agatgaacta
     7141 ttaatccacg gaatcgtcga tggataccta gaagaaaaag atcatctcaa tatctatgat
     7201 tttaaaacag attttatttt acatccggat gatccagcag aaatagatgc aatcgtccaa
     7261 aaatatcaag gacagctaag actttaccaa caagcaatgt cagaagcttt gaataagcca
     7321 gtagaaaatg ttttcctaat tttgcttcga gtgaaacaaa ttataaacat taacaaataa
     7381 ttataattga gcaaacttga cttatgtttt gtaaggatta tgctatactc tgggaagagg
     7441 tgagtatgat ggagctaaca cagacagaat tcgttttatg ccctaaattt gaaaaaacat
     7501 tctctatttt aggaaaaaaa tggaacggat tgattatcga tgtactactc gaaaacggtc
     7561 cgcaacgctt tgttgagctt gcggcaaaaa tcccgatggt cagtgatcga gtattagtcg
     7621 aacgcttgaa ggaattagaa aaagaaggaa tagtcactcg aaactttgcc tgtagcgaaa
     7681 agagccgttc agaatatctc ttgactgaaa aaggagcaga cttgcaaaaa gcgatgcgtc
     7741 aaatacaagt ttggagcgaa aaatggatca cagaggaaga atgcggttga ttttgtaaaa
     7801 aaaatcttgt aaactatagt ccatacgaat aagtaaagaa cgatgacgaa aaagagtagt
     7861 ttggctaaac cagttcaggg agatctgtcg cagactgaaa gcagatcctg tgtttgaaaa
     7921 atgaagttca ctttccgagt tcgtctgata aaatcagacc ggcaattgtc gttaacgaag
     7981 taagagtgcg gtgtaagtca tttatccgca aattaggatg gtaacgcgaa gacctcgttc
     8041 cttaacagga atgaggtctt tttgttttgt atttggaatt taaaggagga acaagcacat
     8101 gtctctacaa gcacaattag agagcttgaa agaagcaacg ctcgtcaaaa tcacagcggc
     8161 acaagattta aatgaattaa atcaaatacg tgttgaaacg ttaggaaaaa aaggaccgat
     8221 cacagaagta ttacgtggga tgaaagatct aagtccagaa gagcgaccaa aagtcggaag
     8281 tttcgcaaat gagatccgag atcttttgac agaagagatc gtgcgaaaaa aacaagtatt
     8341 agaagaagaa gcgttaaatg cagctttggc aaaagaaaca atagacgtga cactacctgg
     8401 tagaaaagta aaacatggta cacgtcatat cgtgacacaa atcatggaag aaattgaaga
     8461 tatttttgtg ggtatgggct atcagatcgt agaaggatat gaagtagaat ctgatcatta
     8521 caatttcgaa cggatgaatc tgccaaaaga tcatccagca agggacatgc aagatacttt
     8581 ctatatttca gacgagatat tgattcggac acatacttca ccagtccaag cacggacaat
     8641 ggaaaaacat gatttctcta agggtgctct acgaatgatc tcgccaggga aagtcttccg
     8701 cagagataca gatgatgcga cccacagtca tcagttccat caaattgaag ggctagttgt
     8761 tgataaaaac atcacaatgg gcgaccttaa aggaacatta gaagtcgtaa tgaaaaaaat
     8821 gtttggggaa gatcgtgaaa ttcgtttgcg tccaagttat ttcccattta cggaaccatc
     8881 agtagaagta gatgtcagct gtttcaaatg tggtggtgcc ggttgtaacg tatgtaaata
     8941 cactggatgg atcgaaattt taggagctgg catggtacac ccgaatgtgt tgaagatgtc
     9001 aggaatcgat ccagaagaat attcaggctt tgctttcggc cttggaccag accgcgtagc
     9061 tatgttgcgg tatggagtga atgatatccg taatttctat caaaatgact tacgtttctt
     9121 aagtcagttc aagggggagt aagaaaatgt tagtttcata taaatggctc aatcgttatg
     9181 tagatttatc gaatgtaaca ccaaaagaat tagcggataa gatgtctgtg acaggaatcg
     9241 aagtagaggg agtaacggtt ccagaagaag gcttgaaaaa aatcgttgtc ggtgaagtca
     9301 aggaatgcgt accacatcca gattctgatc acctttctat ttgtcaagtc gatattggcg
     9361 aagacgaact gtctcaaatc gtttgtggag cgcctaatgt gaaggttgga atcaaagtga
     9421 ttgttgcact tcctaactca cgtatcgctg gtaatgtaaa aatcaaaaaa ggaaaaatgc
     9481 gtggccaagt atcaaatggc atgatttgct cattacaaga aattggctat tcagatagtg
     9541 ttataccgaa agaatacgct gaaggtatct attatatgcc acaagaagct gtaaatggcg
     9601 atccagtttt tccttatttg gatatggatg attcgattat tgaattatct atcacaccga
     9661 atcgtgcgga tgcattgtca atgcgtggcg tagcttatga agttggggca atttatcgtc
     9721 agactcctac attcgatgac gaaaagctgg tagaatcctc acgtcctgct agcgataaaa
     9781 ttcaagtgga agttgaagat aaagaagcag ttcctgctta tcaaatcaga atcattgaag
     9841 gagtaagaat ccagccaagt cctcaatggc tgcaaaatct tttgatgaac gaaggcattc
     9901 gtccaatcaa caatgtagta gatgtgacga actatatttt gcttttattc ggacaaccac
     9961 tacatgcatt tgactacgac aaattgaaat caaaagagat ctttgtccgc cgtgcaaaag
    10021 aaaatgaaac attagtaaca ctagatggag aatctcggga attgacaact gacaatctcg
    10081 tcattactaa cggcaaagaa ccagttgctt tagctggtgt catgggaggg ttagactcag
    10141 agatcacgaa tgaaacaaca acagttgcac tagaatctgc tttatttgat ccaatttcga
    10201 ttcgtcgtac agcaaaacaa tttaatttgc gtagtgaatc ctctgcacgt tttgaaaaag
    10261 gaatcaacca tgcgactgtt ggagaagcca gtgaggttgc tgcagcaatg atcgtttctc
    10321 ttgcgggtgg ggaagttcta caaggcgcgg tcaaaggctc agagatcaaa gcagaagacg
    10381 tatcagtgaa gatttcactt gaacgcatca atcaatattt aggaacatct cttgtcgtta
    10441 aagaagtaaa tgagattttt gaagcacttg gatttagcta tgaagaaaag gagtatgttt
    10501 attccgtagt agtaccacca agacgctggg atatccaaat cgaagcagat attatcgaag
    10561 aagttgctcg tatttatgga tacgaccagt tgccatctac acttccaagt ggagaaacgg
    10621 ttgctggaag cttgtcaaaa gaacaagaaa ctactcgtaa aattcgtaca atctttgaag
    10681 gaagcggatt gagcgaagca atcagttatg cattgacaac agaagaaaaa tcaagacagt
    10741 ttgttataaa tgaaagtaat attacacgtt tggactggcc aatgagcgaa gaacgttctg
    10801 tattacgaat gaatttgatc agcggactat tagatgatat cagttataac actgcccgaa
    10861 aaaatacaga gattgcttta tatgaaattg gtcgggtgtt cttccaagaa aatgatccac
    10921 gtacacatct gccgcaagaa gtaaaacatg cagcaatcgc tatttctggt atttgggaag
    10981 aaaaagattg gcagacgaaa gctcaaaatg tagacttttt caccatcaag ggtcttttag
    11041 aaaacttatt tgaacaacta ggcatcactg aagatgtcca ttatcaaaca gtgacagaaa
    11101 tgaaggaaat gcatcctgga agaactgctg cgatttacct tggcgaaaaa tttattggtt
    11161 ttgtcggtca agtgcatcca acgattacaa aaagttatga tattccagaa acatatgttg
    11221 ctgagtttga tttatctgct atagtggaag ctgctcaaaa agggatcgtt ttcgaagcag
    11281 tgacaaaatt cccagcagtc agccgggaca tcgctttgct tgtaaaagaa actgtgacaa
    11341 atcaggaatt aacagaagtt atccgttctg ctgccggaag atttttaaca gatatccaat
    11401 tatttgatgt ctatcaagga gaaaacattg aaaaaggaca taaatccatg gcgtatagcc
    11461 tgacattcgt caacccagaa gcaacattga cagatgaaga aatcaataaa gcaatggaaa
    11521 aagtcgaaaa agcgttggta gaaaacctag aggcaagtat tcgctaagaa ggaaaatata
    11581 gtaagtaacg atcaagtagc ttttgaagac catacatttg aaaaaaagat gtgacacaac
    11641 ttcgtcacat ctcttttttt gattttaaaa aaggaatagc acagagacga gataaaaaaa
    11701 caggccttta cagcctgctt ttttagagta aatgaattaa tggaagtaat tgattcccat
    11761 tgctgatttt acttcagaaa gtgtttgtgc agctacttta gcagcttctt cactaccttt
    11821 acgtaagatt tccataacag ctgaaggatc tttttccaat tcttctcggc gcgcacggat
    11881 cggtgcaaat tcagcttcca atacatcaat gaggtatcgt ttgatcttta catcaccaag
    11941 tccgccgcgg cggtaatgag ctttcatttc ttcaattgct tctttatctg ttccgaatac
    12001 atccaagtag gtgaagacca tattcccttc tacttgtcca ggatcttgta catggatgtg
    12061 gtttggatca gtatacatac tcatgacttt tttctgtaag acatcggcag gatcggagat
    12121 ataaataccg tttcctaacg atttactcat tttgccattt ccatcgattc caggtaatct
    12181 acccattcct tttggtggga atacgccttt tggttccacc aagacttcac catatgtatg
    12241 attgaagctt tgtacgattt cctgtgtctg ctctagcatt ggtttttgat cctcaccaac
    12301 aggtacaaga ttcgctttga atgcagtgat atctgctgct tgagaaacag gatagataaa
    12361 gaatcctgta ggcacgcttt caccgaattt tttttgttcg atttctgttt tgacagtcgg
    12421 attgcgccga acgcgtccta cgctgactaa gttcaaataa tacatggtta attcagctaa
    12481 ttccggaatt tgtgattgga taaataaagt agattttgca ggatctaatc ccacggctaa
    12541 atagtctaaa gcaacttgca agacatttga tgaaaccttc tcaggatttt tagcattatc
    12601 tgtcagtgct tgcatgtcag caatcatgac aaacagttgg ttgtcttcat ctgcctgcat
    12661 agccactcgg gtttttagag aaccgacgta atgccctaaa tgcagcttcc cagttggtcg
    12721 atctcccgtt aaaataatat ttttcaaaaa agacacctct ttacgatttg taagtctgag
    12781 cacttaatat cttaccgttt ttcctgaaaa gaaac
//
LOCUS       DAZKCY010000071        12727 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000071 DAZKCY010000000
VERSION     DAZKCY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.072"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            235..804
                     /locus_tag="ACG8XC_001856"
     CDS             235..804
                     /locus_tag="ACG8XC_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFP9191548.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            791..1651
                     /locus_tag="ACG8XC_001857"
     CDS             791..1651
                     /locus_tag="ACG8XC_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9191549.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            1841..2545
                     /gene="yycF"
                     /locus_tag="ACG8XC_001858"
     CDS             1841..2545
                     /gene="yycF"
                     /locus_tag="ACG8XC_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFP9191550.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2550..4385
                     /gene="walK"
                     /locus_tag="ACG8XC_001859"
     CDS             2550..4385
                     /gene="walK"
                     /locus_tag="ACG8XC_001859"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFP9191551.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4382..5698
                     /gene="yycH"
                     /locus_tag="ACG8XC_001860"
     CDS             4382..5698
                     /gene="yycH"
                     /locus_tag="ACG8XC_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFP9191552.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5699..6568
                     /locus_tag="ACG8XC_001861"
     CDS             5699..6568
                     /locus_tag="ACG8XC_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFP9191553.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6623..7432
                     /locus_tag="ACG8XC_001862"
     CDS             6623..7432
                     /locus_tag="ACG8XC_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9191554.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7535..8164)
                     /locus_tag="ACG8XC_001863"
     CDS             complement(7535..8164)
                     /locus_tag="ACG8XC_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9191555.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8334..9626
                     /locus_tag="ACG8XC_001864"
     CDS             8334..9626
                     /locus_tag="ACG8XC_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9191556.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9702..10364)
                     /locus_tag="ACG8XC_001865"
     CDS             complement(9702..10364)
                     /locus_tag="ACG8XC_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP9191557.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10589..10873
                     /gene="groES"
                     /locus_tag="ACG8XC_001866"
     CDS             10589..10873
                     /gene="groES"
                     /locus_tag="ACG8XC_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP9191558.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            10924..12549
                     /gene="groL"
                     /locus_tag="ACG8XC_001867"
     CDS             10924..12549
                     /gene="groL"
                     /locus_tag="ACG8XC_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP9191559.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4231 a         2189 c         2764 g         3543 t
ORIGIN      
        1 aaacaaagtt cttgcaagag aacaaaccta acactttttt cattttttac tcctttcccg
       61 ccgtctcccc ggcgggtctc ctgttcttaa cgataaaaga caagaaggct tagccttctt
      121 gttttttgtt ttcatggaat cccgctaatc aaatcaccta gttaaagcaa ctagattgaa
      181 tgtttatcat aagtctgata tcatgtttat gaaatgaaaa aaggagcggt tatgatggaa
      241 aaaatgcgtc agcaattaat ggagtggggg aaggaactag aaacattccg tctgcctcac
      301 tgggaagaac tccctgactt agaactttat atggaccaag tcagaacgtt agttgatcgt
      361 tatctttcac ctgtgataca aggagaaaag catccattgc ttacctcttc tatggtaaac
      421 aattacgtta aactaggttt gataccagca ccagttaaaa aaagatataa caaagaacac
      481 gtagcatttt tacttgcaat cactacgttg aaacaagtat tgactattcc tgaaatcaaa
      541 gaagggatat tgttccaagg aaaaacagta ggaattcgtg aagcctataa tctattttgc
      601 gatgaacaag aagcagctgt gtggatggtc agccaattag cacaaggtaa atctcatccg
      661 caaaaattcg accaggcaac ccctgtagaa tatattgctg taagagcggc aactttatcg
      721 tttgcaatga aacttcttgc ggaaaaaacg attgttctag aaaccgaata tttaaaagag
      781 gagaaaacaa atgaaaaaca ataaaatcgc aattttagta gattcaggta cggatgtgcc
      841 agaagaattg ataaaaaaat acgagatgta tatgatccca ttgaaaatta tttataaaga
      901 tcaggtatat acagataaaa ttgacattac ccctgaagag atctatcaac gtttgccaga
      961 ggaaatccct ggtacatctt tgccggatgg tgagtcgatc acagaaatct ttgagcaaat
     1021 caaaaaagac ggttatgaaa aggtacttgc ggtaacaatt tccagtggat tgagcgggac
     1081 atataatgtg gttcgtttaa ttgcacaaca gcaagaagaa ttggaagcat atgtactaga
     1141 tacgaaaaat attggtattg gtgcaggttt ttctgcgatc caagcagcaa aatggctgga
     1201 agaaggagta gaatgggatc aactgatcag taatctgaat gagttagtcg aaagaacaaa
     1261 agttttcttt aatgttgcga cattggaata tcttcaaaaa ggtggacgga tcggtttagt
     1321 tgcttctatt gtaggaaccg ctttaaagct taacccaatc atctcatgta atgaagaagg
     1381 gatttattac acagtaggaa aagctagggg acgtaaaaaa agtttggata aaacggttgc
     1441 ttacgtcaaa gaaaaaatcg gtaatgcaaa ggtcttcaac ttagctgttg cccatgggga
     1501 tgcgaaagaa gaagcaattg aaatggaagc gagacttaaa agagagttcc cacaagcaaa
     1561 ccaaatctac tttggccaga tttctccggc attagtagtc cacactggtc cgggattatt
     1621 gggtgtcggt gtacaagttt tagaaggata aatcaaaaaa aggaaacact gaggtgaaaa
     1681 tcagtgtttt ccttttttat tgaagttaga acaaaaaata ataaagtatt ttgcactttc
     1741 attttaattt tctcttttgt tttataggca aagatgaaac aaaagcagaa atacggtata
     1801 atgagaaggt atgacaataa tatataagag gtggctcttt atgaaaaaag ttttagttgt
     1861 cgatgatgag aagccgattt cggatatcgt caaattcaat ttagccaaag aaggctatga
     1921 tgtttatact gcatatgatg gggaagaagc cttggaaaaa gttgctgagg tagaacctga
     1981 tttgatttta cttgatttga tgctacctaa aatggacggc ttagaagttg ctcgtgaggt
     2041 acgcaaaact tatgatatgc cgatcattat ggtaacagcc aaagattctg aaattgataa
     2101 agtcctaggc ttagagttag gcgccgatga ttacgtgacc aagccatttt ccaaccgaga
     2161 attagttgca cgtgtcaaag caaatcttcg tcgcggtgcc acggctgcga aggaaccaga
     2221 agaggcagca cctgcggaat tgacgattgg tgatttgacg atccatccag aagcttacat
     2281 ggtgacgaaa cgaggagaaa caatcgagtt aacacatcgt gaattcgaac tactctttta
     2341 cttggcgaag caccttggac aagtgatgac aagagaacat ttattacaaa cggtttgggg
     2401 ttatgattat tttggagatg tacgaacagt ggatgtaact gtacgtcgtt tgcgtgaaaa
     2461 gatcgaagat aatccaagcc atccaaatta tttagtgaca cgtcgtggtg tcggttacta
     2521 tttgcgtaac ccagaacagg agtaattgga tgaaaagaaa agtccgcttt tttcgatcag
     2581 tcaattttaa gatcgctatt acctttattc tgattttatt gatctcgatc gaaattattg
     2641 gtgcctactt tatacgtggt ttagaacgtt ccacgatcaa tacatttatt aaagatatga
     2701 atcaaacggt ggaatcatta gcaacaacaa tcagcccgga attgaatcga aaagataatg
     2761 cagatgatga agaggtaaat gcgaatatca aaagatttat tgaaaacagt gcgactagcg
     2821 atatcatcga aatacgggtc gttgacgaaa aagggatcat ccgaggaaca accgatgtca
     2881 atgaacaaag cgctgtggga aagaaaaatg attatgttga tatcaatgat ttcacgacta
     2941 aacgttatgt tgctttagat aacgacgata aacgggtgaa tatcaatgtt caaccgatcc
     3001 tgtccccgac cggcgatgcc gttatcgggg ctctttacgt caaaagtaat atcgaacaaa
     3061 aatattcgga aatcaataac acagcagtca ttttctttac tgcctcacta attgcggcat
     3121 ttatttcaat gatcgtatct gttttagttg ctcgttcgat tacacagccg atcggtgaaa
     3181 tgagagaaca agccattcga atcgctagag gagactacag tcggaaagtc aaagtgcatg
     3241 gacaagatga acttggacag ctggctgata ccttcaatca gttagctgaa cgtattgaag
     3301 aagctcaaga tacaatggaa gctgaacgca atcgtttgga tagcgtgcta tcccatatga
     3361 cagacggcgt cattgcgaca gatcgcaggg gtaaagtcat tacgatcaat gatatggctg
     3421 tttccttgct agatatcaaa aacgaggaag caatcggaca atcgattttg acacttttag
     3481 atatcgaaga agaatatacg ttgcggaaat tattagaaga tccaaacgaa atggtacttg
     3541 accgttcaac cagtacattg gaaagtgatc agatgatctt gcgagtagac ttttctatga
     3601 tccgtcgcga atcaggtttt atcagcggat tagtagctgt cttgcatgat gtgacagaac
     3661 aagaaaaaac agaacgggaa cgtcgtgaat tcgtctctaa tgtttcccat gaacttcgta
     3721 caccgttgac aagtatgcgt agttatatcg aagcattgag cgaaggtgcg tggaaagacg
     3781 aggaagtagc accaaatttt ttgaaggtaa cgctagatga aacagaccga atgattcgaa
     3841 tgatcaatga cctcttaaat ctatctcgca tggatacagg taatacccag cttcaattag
     3901 aatatgtcaa tttcaacgag ttagtgaatt ttgttcttga ccggtttgat atgatggtgg
     3961 gaaatcagga aaaaaattat aagatccgtc gtgaatttac gcaaagagat ctttgggtcg
     4021 aagtagatac agataaaatc atccaagtag tcgataatat catgaataac gcgatcaagt
     4081 attcaccaga tgggggaacg attacttgtc gcttattaga aacgcataat aacgtgattt
     4141 taagtatcac cgatcaagga ttggggattc ctaaaaaaga tctgaaccga gtgttcgagc
     4201 ggttctatcg agtagataaa gcacgtgcaa gagcacaagg tgggacagga cttggattag
     4261 ctatttctag agaagtgatt aaggcgcatc gaggcgctat ttgggcagag agcaaggaag
     4321 gaaaagggtc gactttttat atctcgctgc catatgaacc ttatgaggag gaatggtggg
     4381 aatgagaatc tctgaaaaag tcgttcgtat aggactcatt ttgatggttg cgttaagcat
     4441 atacttctct tatgcaatct ggctgagccc agcaggaaaa agttcaatca acgtagatga
     4501 gacaaacact caaatgatcg agtctcaaag caatcgaaaa acttccgaag tctttcttcc
     4561 gcttcatgtt acatggcacc attcgggaat gatccaagaa accaacagtg aaaatctcgt
     4621 gtaccgattg caattgatca tcgataaagc aagatttgga aagctttctg aagtagtttc
     4681 aggagacaaa tcacagtttg aacaggtgaa aaatctgaac tctggttttg agttggccta
     4741 taatgcgcct tttttgctaa gagaatacaa ggaagcattt gatttagatt tagatgtgtc
     4801 gaacttggca aaagaaagcg aaaatatgta ttttactaga atacaattcg atcaaggaaa
     4861 taaaaaaatc cgatttttga attattcgaa ccatctcgta tatgaagcaa atatttcgtt
     4921 gaattggaca gatatcaata aagaattaac agcaaaagac acaaaatgga cagaaatgac
     4981 aacagaacca tcgacgatcg acacgcaata tgctacgaaa cattcgatca aactgaaaaa
     5041 gtatagttat atcctgtcac agcaacccta tacacttttt agaaatgctt ttttccagaa
     5101 accagatagc gtgaaaaata atgatgaaag caatgaattg attttttacg gtgggaatga
     5161 aacaatgtct atccattcgg aaacgcaagt agttgatttt cgtggagagc tgccagagaa
     5221 aaaagaaaat gctagtgtat tttctgaaag tttcccctat attagcaaat taggcagttc
     5281 tttagggaac ctcagatatt ttgaccgcag tgacaatacg atcgattatc ggatattcgt
     5341 ggaaggcttc ccggtttttg ggacagacag cagaggacag attggtgtgg aagttggcgg
     5401 tgaaacgaat ggttctgtgc aagtgaatat ccaaaccagt ttgaatacga ttcaagtgcc
     5461 gataccttca gacgaagaag tagaacttgc aagtacgaat gaaatcatcc agcagttaaa
     5521 agctaaggga gcagatagca acaagattgg ttcagtgatc gttggctata catggcaaaa
     5581 tgtcaaagaa acgaatcgtg tcgttgatct cttacctgaa tggtatgtga aatataaaga
     5641 taactggtat tcagctaacg accttctgga aaaattacct ggattggagg cgaattaatt
     5701 ggatttcaaa aaaatcgaat ggatattttt cgtcgctttt ttagggttga atgtgttttt
     5761 attcagcagt tatcatgacg cggtatacca agaaaacaat gtcattcgct ctaatcagac
     5821 tgaatcaatc caacaacgtc tggctagcga cgatatcact tacacgggga agatttcgtc
     5881 tgagaacaag caaggctatt atcttagcgc ggagcagacc aatcttagca cttcattagc
     5941 gaattatcgc aaggctggta aaagcggctt gctggcaagt ggtgtgtttt tagacggtga
     6001 tgttttgacg aaatctttat ccagttcaga tgctgaaaag aacgtgatcg atccagataa
     6061 aattagtagt tccttggaag attttttatc taacaaagac aatattttat tcggtaatga
     6121 ttatgtgtac acgaaaaact tttctcattt agaagaagaa ccactagaaa tccaagcaag
     6181 ccaagagtat gaaggcatcc cattcaatga tgatacagca aaaatcgttc tttctttaga
     6241 aaaaaacgat aatgattata gcatcacgaa atacatgcag acgcatttat ctgatatcga
     6301 gcagctgcgc gaaaagacgg aactgtatac ggaagaggaa gcaatcaata cattgtacat
     6361 caataacaaa ataccaagag gatcaaaaat tttatggcgt cagctagctt attcctgtat
     6421 tctgaaagtc cgggaaaaaa atgtgtatgt tcctgtttgg catgtagcga tcgaaacaag
     6481 cgataaagtg gtccaaattg agagcgtcaa cgctttcagc aatacgatcg tgacaaacaa
     6541 tactgtacca aaagtggaag atcaataaaa aagcgagtat aatgaaaaaa gtttacaaag
     6601 taagaaggga agtaggagtc aaatggctga agaaaaacca gcttttaaaa tcagtatcct
     6661 agcaagtgga agttcgggta attcacttta tatcgaaagt gaaaaaaaac ggcttttggt
     6721 cgatgcagga cttagcggta aaaaaatcac ttccttgatg gctcaaattg atcggacacc
     6781 ggaccaatta gatggaatac ttgtgacgca tgaacatcga gatcatatac atggcgtggg
     6841 tgtcttggct cgtaagtatc atttggatgt ttttgccaat cagcagactt gggaagcgat
     6901 ggaaccactg attggaaatg taccagtgga acaaaaacat ctatttgaga tggggaaagt
     6961 cagaacattc ggcgatatcg atattgagag tttcggcgtc tcccatgacg cagcggctcc
     7021 gcaattttat cgtttccata aagaaaatcg gtcattcgtc atgttgacgg acaccggata
     7081 ttgcagtgat tatatgcgag gtgtcattga aaatgccgat ggttatttga tggaaagtaa
     7141 tcatgatctt gagatgttac gcatgggacc ttatccgtgg aatttaaaac agcgtatttt
     7201 aggagataga ggacatcttt ccaacgaaga cggtgcactt gtcatgactg atgtgatcgg
     7261 taatcggacc aaacgaatct atcttggcca tcttagcaag gaaaacaata tgaaagaact
     7321 tgctcattta acaatggaaa atgttttgaa agaaaaagat tttggcgttg gacatgattt
     7381 tgaaatctat gatacagatc cagatgaagc aactgagcta ttttcgattt aaacagttca
     7441 atcaataaaa atataaaaag cagaagataa aatcaatcag ggttggtttt atcttctatt
     7501 tttatttttt tagcatatta aaaaaataac cgtattatcg actgactaca ctgatgtgct
     7561 gcttttcatg tttctcatcc agtatctcat ctactaatgc aatcgcgtaa tctgccatgc
     7621 tgatcgtact gtcgccagca gcgtttcttt gtaaataatc atcacttaac aaataagtac
     7681 ctgtacgttt gccatgtgga tcaaataaag aagctggact gatataagtc caatgtagat
     7741 ttttagattg tttcaatttt aacaatcctt cgtacatatt gaaagctgtt ggatagaagg
     7801 gtgcattttt aggtacttgg tcaatcatac gagtcgtctt ttctgtatct aagaataacg
     7861 aagaggcacc cccaacgaca attaagcgtg tatcggttcc ttcaagcatc tcaatcaaat
     7921 gggacaaaga agtttgatgc atttcttctt ttcctttggg agcgttgaaa gcatcaacga
     7981 caacatcaaa agatgcaaga tcagtagttg tcaactcaaa taaatccttt tgtataaacg
     8041 gaacatctga tggcaatttt tctggatgac gcacgattgc cgtcacgtct aaatctctgg
     8101 acaatgcttc gtctaaaata aatgaacctg catggccagt agctccaatg agtgcaattt
     8161 tcataaaaaa cctccttttt tattcctgtc cattgtaagc agttgctgaa tattcgtcaa
     8221 agaatccgat ttttgaaaag ctcagtgcgc attcctttca tggttcgtga tcattgggta
     8281 tagttaaatt aagaaaactg attgataaaa atagaagttg aaggagataa gaaatgaaag
     8341 agtggttaga tcgtgccttt ttaccaatga taacatctgt tgtgaatctt agacccttaa
     8401 ctgctttgcg taatggaatg acttacacga tcccaataat tattgttggc tcgctgtttt
     8461 tcttatttgc tcattttcct gtggattaca tcgctcagtg gatagacaaa acaggatttt
     8521 ctatgtattt tacacaagta taccaggcta catttgatgt gatagccttt tggatcgttt
     8581 ttgccattgc atacgcttat gcaaaagaag aagacatcga tcgcttacca gtcggcataa
     8641 cagccttgtc tgcttatcta ctgcttattc agccactaaa tactcagata gatactgtct
     8701 ggacaggtgc tgaagggata ctaggagcag ttacaactgg gctcatcatc ggatggggct
     8761 atgcacgttt agtaagactt gcgaaagggt ggaagcaaag agagaagata cctgaaaatg
     8821 tttggcagtc ttttacttca ttgattccaa tcattgttat agtcactttt acgcttatcg
     8881 gttcagcggt ttttcaacag ttgttcagaa tgactgttgt tcaaggagta tggacatatg
     8941 tccagcttcc cattcaatcg ttcatagaca cgttgtttgg tgtgctttta ttaggttttt
     9001 ttgttccgtt tttatggtta tttggtcttc atggatctgc gatggtcaat gggttggtta
     9061 gtcccgtctt gcaagcgaat tcattagcaa atgcagaaat cttagcctca ggacaagagt
     9121 tgacggtggc aaatggcgga catatcgtta ctcagcaatt tctcgatcag ttcatgacgg
     9181 ttacgggtgc tggattgacg ttaggggctg tattttttat gacggttttt gcgaaatcga
     9241 agaaatacag agaattagga aatcttgctc ttttaccagc ctttttcaac atcaatgaat
     9301 caattatttt ttccacaccg atcgtgatga acccgatgat ggctgttcct tttattttcg
     9361 caccggtcgt ttcaggattg attacctatt ccgctttata ttttggattc gttcctttat
     9421 ttacagcagt ccaagtccct tggacaacac cgccgatcct ctcgggtttc ttagtaggcg
     9481 gagtgcctgc agcgatttta caaggtatcg tgttgtcaat tagttttttc atttatttcc
     9541 catttttgaa aaaaatagat tctgcaaact gcaaaaagga aagacagaca aaaaatgatg
     9601 gagcagcaga aaagatgaac gactaaaaaa caaaaatact cctgtcgctt tagattggga
     9661 aaatcaccaa tctaggatga caggagtatt tatagtcatg tttatactag ccctttttgg
     9721 acagctaatt gaacaagaac aactaatgtg ttcattccta cgtgtgtgat catcgatgtc
     9781 cagatccgac cagttgcttt gtactctagg ctgaagaaga aacctaggaa aaagtagatt
     9841 agcagatgac catcattatg agcaaacgca aacagcaaag agctgatcac tgctccgacc
     9901 caaaaatttg aatatcgact gatgatcccc agtatactgc ggcggaatac gaactcttcc
     9961 atgatgggtc cagcaacagt tgtcgccaaa ataaaagcgg gtgcttccat gatcatctgg
    10021 atgatatttt gtgtattctc ggaaggggtt gctttgccaa acaatacaga ttctaacatg
    10081 acagcgatgc tttgcagaat caatgcgaca aagattccaa ccaatccata aataacaatc
    10141 tttggagcag aagctttttt taccgctgat tcgatcgcta agggttcacg ttgcttaaaa
    10201 taaaaaatag ctaggacaac ggcgcctagt atatacatga ctgcagttgc tgtgatcaat
    10261 tcagacgtag attttacaag tcctatcgaa gctaggatca agggtgagat aaaaattaga
    10321 ccatagcaaa aaatgctgag gaagctatat ttttttaaag acatgtaatt cctcctttta
    10381 agggataaca agcatgcaag gcaagcttat atcgattgat ttcaattata tcataatttc
    10441 aataagcagc tgtcagaata aaatttcttt tctttgattt aaaagcttgc aaaaaagagg
    10501 gagaatgagt attattaaaa atgtaggtta gcactcgaag gttaagagtg ctaacagatt
    10561 aaaaaaagag ttatcggagg gatttatcgt gttaaaacca ttaggtgatc gcgtcattat
    10621 cgaagtcgca aaagaagaag aaaaaactgt cggaggaatc gttttagctt ctgctgctaa
    10681 agagaaaccc caaactggga cagttgttgc agtcggtgaa ggccgtttgt tagaaaacgg
    10741 tgaaaaagtt cctgccgctg taaaagcagg cgaccaagtt atgttcgaaa aatacgccgg
    10801 tacagaagta aaatacgaag gaaaagaata tctgatcgtc gcaggaaaag atatcatggc
    10861 gattgttgag taaatttgga agataaaaaa ataatcaaaa ataatcaatg aggtgaatca
    10921 atcatggcaa aagaattgaa atttgcagaa gacgcacgcg cagctatgtt acgcggggta
    10981 gataaattag cagatacagt gaaagtaaca ttaggaccta aaggccgaaa tgtcgtacta
    11041 gaaaaatctt acggctctcc attgatcaca aatgatggtg tgacgatcgc aaaagaaatc
    11101 gaattagaag atcattttga aaacatgggt gcaaaacttg tatcagaagt agcatcaaaa
    11161 acaaatgata ttgcaggaga cggtacaacg acagcaactg ttctgacaca agcaatcgtt
    11221 cgcgaaggtt taaaaaacgt aactgcaggt gctaatcctc taggcattcg tcgcgggatc
    11281 gaattggcaa caaaagcagc agtcgaagaa ttgcataata tctcaactgt tgttgattca
    11341 aaagaagcca ttgcacaagt agcagctgtt tcttcgggtt ctgataaagt tggacatttg
    11401 attgctgatg caatggaaaa agttggaaac gacggtgtca tcacaatcga agaatcaaaa
    11461 gggatcgaaa cagaactaga tgttgtagaa ggtatgcagt ttgatcgtgg ttacttatca
    11521 caatatatgg taacagataa cgacaaaatg gaagctgttc tagaaaaccc atatatcttg
    11581 atcacagaca aaaaaatctc taacatccaa gatattctgc cattgttaga acaaatcttg
    11641 caacaatcac gtccattgtt gattattgct gacgatgtag atggtgaagc attgccaact
    11701 cttgtattga acaaaatccg tggaacattc aacgtagtag ctgtaaaagc tcctggtttc
    11761 ggcgatcgtc gtaaagcaat gcttgaagat atcgctattt tgacaggtgg tacagtaatt
    11821 actgacgatc taggtttaga attgaaagat gcaacaatcg aaaacttagg aaatgcttct
    11881 aaagttgttg tagataaaga caatacaacg atcgtagaag gttctggaga aaaagaagca
    11941 atcgaagctc gtgtgcaatt gatcaaaaac caaattgctg aaacaacttc tgattttgac
    12001 cgcgaaaaat tacaagaacg cttagctaaa ttagcaggtg gtgtagctgt tgttaaagtt
    12061 ggtgcagcta ctgaaacaga attgaaagaa ttaaaattac gcattgaaga tgcattgaat
    12121 gctacacgtg ccgcagtaga agaaggaatg gtctcaggtg gtggtacagc tctagtgaac
    12181 gttatcagca aagtttctgc tgtagaagca gaaggagacg tggcaacagg tatcaagatc
    12241 gttgttcgtg ctttagaaga accaattcgt caaatcgctg aaaacgctgg ttatgaagga
    12301 tcagtgattg ttgataaatt gaagaacgtg gaactaggca ctggtttcaa tgctgctact
    12361 ggagaatggg taaacatggt tgaagcaggt atcgtagacc caactaaagt aacacgttct
    12421 gccttgcaaa atgccgcttc cgtttctgcc ttgttattaa cgacagaagc agtcgttgca
    12481 gataaaccag aaccagcagc gccagcagct cctgcaatgg atccatcaat gatgggcggt
    12541 atgatgtaag aaaaagcaac ttgcttatct acaactgatc attagtgaaa gacagataaa
    12601 aagaggctgg aacagaagcg tttaactcca agaaataaga agaaattcac ggaaattgct
    12661 tttcaaattt ttgtgaattt cagcttattt ccgaaggagt tgcttctgct tccgctgttt
    12721 atacgtg
//
LOCUS       DAZKCY010000072        12223 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000072 DAZKCY010000000
VERSION     DAZKCY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.073"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="ACG8XC_001868"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="ACG8XC_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP9191560.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /gene="pyrH"
                     /locus_tag="ACG8XC_001869"
     CDS             complement(740..1462)
                     /gene="pyrH"
                     /locus_tag="ACG8XC_001869"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP9191561.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /gene="tsf"
                     /locus_tag="ACG8XC_001870"
     CDS             complement(1598..2479)
                     /gene="tsf"
                     /locus_tag="ACG8XC_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP9191562.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="ACG8XC_001871"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="ACG8XC_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP9191563.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="ACG8XC_001872"
     CDS             3719..4198
                     /locus_tag="ACG8XC_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9191564.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="ACG8XC_001873"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="ACG8XC_001873"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFP9191565.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="ACG8XC_001874"
     CDS             5499..5627
                     /locus_tag="ACG8XC_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFP9191566.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5631..6176
                     /locus_tag="ACG8XC_001875"
     CDS             5631..6176
                     /locus_tag="ACG8XC_001875"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFP9191567.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6629..8320)
                     /gene="argS"
                     /locus_tag="ACG8XC_001876"
     CDS             complement(6629..8320)
                     /gene="argS"
                     /locus_tag="ACG8XC_001876"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP9191568.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="ACG8XC_001877"
     CDS             complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="ACG8XC_001877"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9191569.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9801..10820)
                     /gene="argF"
                     /locus_tag="ACG8XC_001878"
     CDS             complement(9801..10820)
                     /gene="argF"
                     /locus_tag="ACG8XC_001878"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP9191570.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="ACG8XC_001879"
     CDS             complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="ACG8XC_001879"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFP9191571.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3348 a         2532 c         2205 g         4138 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaatc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
     6481 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
     6541 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
     6601 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
     6661 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
     6721 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
     6781 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
     6841 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
     6901 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
     6961 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
     7021 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
     7081 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
     7141 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
     7201 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
     7261 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
     7321 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
     7381 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
     7441 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
     7501 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
     7561 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
     7621 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
     7681 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
     7741 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
     7801 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
     7861 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
     7921 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
     7981 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact acggtttgca
     8041 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
     8101 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
     8161 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
     8221 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
     8281 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
     8341 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
     8401 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
     8461 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
     8521 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
     8581 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
     8641 ctagtcgttt cataaaaaaa aatccagatg gcctaaagga gaagaaatac cacctggata
     8701 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8761 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8821 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8881 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8941 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     9001 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     9061 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     9121 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9181 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9241 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9301 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9361 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9421 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9481 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9541 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9601 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9661 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9721 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9781 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9841 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9901 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9961 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    10021 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    10081 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    10141 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10201 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10261 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10321 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10381 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10441 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10501 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10561 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10621 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10681 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10741 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10801 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10861 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10921 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10981 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    11041 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    11101 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    11161 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11221 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11281 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11341 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11401 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11461 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11521 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11581 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11641 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11701 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11761 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11821 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11881 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11941 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    12001 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    12061 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    12121 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12181 tcctcctttt gtaatcactt tcataatttt gtctcattca aaa
//
LOCUS       DAZKCY010000073        12091 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000073 DAZKCY010000000
VERSION     DAZKCY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.074"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..1014
                     /locus_tag="ACG8XC_001880"
     CDS             76..1014
                     /locus_tag="ACG8XC_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9191572.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            1026..1937
                     /locus_tag="ACG8XC_001881"
     CDS             1026..1937
                     /locus_tag="ACG8XC_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9191573.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            1961..3433
                     /locus_tag="ACG8XC_001882"
     CDS             1961..3433
                     /locus_tag="ACG8XC_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9191574.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            3484..4095
                     /locus_tag="ACG8XC_001883"
     CDS             3484..4095
                     /locus_tag="ACG8XC_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9191575.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            4125..6038
                     /locus_tag="ACG8XC_001884"
     CDS             4125..6038
                     /locus_tag="ACG8XC_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFP9191576.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            6166..9147
                     /locus_tag="ACG8XC_001885"
     CDS             6166..9147
                     /locus_tag="ACG8XC_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFP9191577.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            9144..10805
                     /locus_tag="ACG8XC_001886"
     CDS             9144..10805
                     /locus_tag="ACG8XC_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFP9191578.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            10845..11777
                     /locus_tag="ACG8XC_001887"
     CDS             10845..11777
                     /locus_tag="ACG8XC_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFP9191579.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         3966 a         1915 c         2504 g         3706 t
ORIGIN      
        1 taatgtataa aaaattaatg taagcgatta accaagtggg tataatcaag gaaacgctga
       61 gagaggagag acaaaatgaa gctaaagaaa gggttattag gaaatctaaa aaaaaaccga
      121 gcattgttta ttatgctttt accaggtgcg ttatggttat ttatattttg ctatatacca
      181 atgtttggag ttgtaatcgc ttttaaagat ttcagaattt catcaagggg attctttaca
      241 agtttgttca acagtccttg ggttggctgg gaaaatttca aatatctctt tgcttcgcaa
      301 gatgcttgga tcattactcg caatacggtt gtttataacg ttgtaggtat tttgctaagt
      361 actttttgta cagtgtcatt agcaatcatt ctgagccaaa tggcgaacaa acggttattg
      421 aaaaagatgc agacatttat gatttttcct aattttttat catgggtagt catcagttac
      481 tttgtttacg catttttgag tacggataaa ggattaatta atattttact aagcaatttt
      541 ggtgtggaac ccataaattg gtatagtgaa ccgaagtact ggccttttat tattgttttt
      601 acgagtttat ggaaaggcgt aggctatgga tccatcattt attacgcgtc aattatgggg
      661 atcgatacta gttattatga agctgcttta gttgatggtg ccagtaaatg gcaacagata
      721 aggaatgtag tcttgccaca tttaagatcg attattgtca ttatgttgat tttgtctgtt
      781 ggtagtattt ttcgagctga ttttggattg ttttatcaat tgccaagaaa ttcgggggct
      841 ctttatgatg tgacgaatgt attagatact tatgtctacc gtggattaat gaatagcgga
      901 gatatcggaa tgtctacggc agcgggatta tatcagtcat cagttggctt tgtcttgatt
      961 ttgattacta ataagatagt caacaagtta gatcctagtt cagcactatt ttaggaggaa
     1021 tcaaaatgat atcaaaatta aaacggcaga tgtacttttc gctgccagtc gaacggtttt
     1081 tcacaatatt actaatctta tttactctta gctgtatttt tccctttatt ttcgtaatcg
     1141 tcatttcatt aacagatgaa aaagcattac aactcaacgg ttacgaatta ttgccggctc
     1201 aatggagcac tgatgcatat caatacttaa tacaagatgg cggacagtta cttcggtcat
     1261 taggcgttac aattatgatt acagtgattg gaacgttgat tactgttttt atgacgggaa
     1321 cctatgctta tgttttatca agagcctctt tcccttaccg gaagttcttt actttttatc
     1381 ttttttttac catgttattt gcgggaggga tggttccttc ttacttagtg atgacgaaga
     1441 tgcttggctt aaaaaataca atttgggctt tgattctacc tcttgctttt agcccataca
     1501 atgtcattat tttaaggaca ttctttaaga agtcgattcc agaatcgatt atcgaatccg
     1561 ctaaaatgga tggctgtagt gagtttcgag tttttttcca aatcgttttg cctttagcga
     1621 tcccaggggt tgcaacaata ggtttatttt caagcttagg ttattggaat gactggttca
     1681 acgcattgct gtatattgac accaacaaat tgattccatt gcaatatttg ttaatgaaga
     1741 ttcaaaattc aatggaattc ttagcaaata ataatgacat tacattagcg cagcaacaag
     1801 caattcaaaa tagccttcct caagaatcaa cacgaatggc aatggtcgta gtagctactt
     1861 taccgattgc aattgtttat ccgttcttcc aaaaatactt tgttcaagga ttgacgatcg
     1921 gtggagtgaa agaataaaaa agaataggag atgttaaaag atgaaaaaga acatgcagaa
     1981 atatttgatt ggtggtgctt ctgtactaac ccttggccta ttaggtgctt gtggtagcag
     2041 taatggcgaa gcaaataatg aaacaggaac cacaaaagtt acttggtatc aggttggaga
     2101 agaacccaaa gattttgatg aggtcatgac aaaagtcaac gaaaaattaa aggaagaaat
     2161 caatgtagag ttggatatgc ggctgattcc ggacggggat tatcagcaaa agcttggtgt
     2221 gatgatcaat tcaggagaag aatatgatat ttgtttcgtc aatggcacag attatgtgaa
     2281 ttatgggaac aaaggtgctt tcattagtct caacgatcat ttggataaag agttgaaaga
     2341 ttatgcagct gaattgaatg aaggatttat tgaaggtggg gcaattaatg gcgaaactta
     2401 tggattgcca gtcaataacg atattgccac aacggaatat tggaccttta ataaagattt
     2461 ggtagatcaa taccaattgg atgtagagaa tgttgaaggt gtagaaacat tggctccagt
     2521 attagaaaag tttgcagaaa aaaatactga tacgaatatt acgccaatcg gtatgacccg
     2581 taccagtgtc tgtattcctt atgacaatat tatcggtggt ggtgtaccgc tagcggttgc
     2641 atttgaaggc gatacacaaa cagttgttaa tacttatgag actccagaaa tgatgactta
     2701 tttgaaggct atgcatgagt tttataaaaa aggatatatt ttgaaagatg cagccactgt
     2761 tacgacagat ccatttcgaa ttgatagtaa aaactggttt ttaggtcgta actcaagtgg
     2821 ttatcaacca ttagcacaaa aaaaattaga agctctagcg ggacatggat taattttaaa
     2881 accagctgga aaagctttta aaacgactgg atttgcaaga gtggcaatgc atgcggtatc
     2941 tgcttcaagt aaacatccgg aagcagcctt gaaattgttg aattatgtga ataccaatca
     3001 agaagtagcg gatttaatgg tttatggaat tgaaggaaaa cactatgatc gcaatgaaga
     3061 tggttcgatt actttaacaa actctgatga ttattatcaa ggcgcttggg cattggtaaa
     3121 tacttatcta gtttcaaaaa cttcaccgat tacagaagaa agtaaaaaac tagaagaaga
     3181 aagtaaaaag tttcaaaatg aagcaccgat gtctcctatt ttaggtttct cttttgacgc
     3241 ttctgatttt cgaacagaaa ttgcaagtgt acaaaatatc gtgtcacaat atgaagtact
     3301 tttaagctcg ggaacagttg accctgaaca atatgtacca gagttgttag aaaaactaga
     3361 aaaagctgga ttggataaaa ttcaggcatc tgctcaagaa caatacgatg aatggcgaaa
     3421 atctaacaaa taatttttta ctaaatgaaa agaatagact aagcagttag tggggggatt
     3481 caaatgaacg aaaacaaatg gaccaaagga tttaattggg tttttcaatt gttgttacta
     3541 aactttactt attttatact aatttgttta ggatttggaa ttttaggttt tttccctgct
     3601 acgatagctt tgattgctgg cgttgtgaat cgaaaaaaat atggcttgca agagtctgtg
     3661 aagttcatga gtaaaatcta taagaaaaat tttgttagat ataatctata ttgctggtca
     3721 gcaactttgt tgttggccgg caatattact acgatctggc tttgttggca gcaaagcgca
     3781 ccattgtttt atttattaag tacattatta tcggtaatta cactgttcct agtctttgca
     3841 atgttgaatt gggtcgtgat caaaagtctt gggttgtgga tgatgggaga ccgactgatt
     3901 gactatttgc ttttcacagc aacgatgttg ccaacggtag tcatgcaagg cttggtgctt
     3961 ggaatttatt tccgaatatt gatgattttg cctgtgattt ttctgtttgg cggaacagct
     4021 gtaacggtaa tattgattag taaattaact gaaagagcgt tagcaaagtt gccttgtcag
     4081 atacaatcgc actaattttt tcacaaaaag gagtatttct ttctatgaag ttaactgata
     4141 aacaagtagc taatatagcc tttatccagc aacttgagat tccattgaca caaaaagatg
     4201 aagaacaaat agacttgatt tttactgaca caaaggctaa tgtgcgacaa atcaagatta
     4261 ctaaaaatga gcagcaacga atttgcttgt cttattcaca tgaagacttg ctgtatcgag
     4321 gattgtttat ctgccttcca cgttttttag ctcaaggagg agagatgcca tacattgaag
     4381 aaacctcgat cattgagacc agttattcat tagatgcttc tcgtaatggc gtgatgaata
     4441 aagagactct aaaaaaattc atttcttact tagcgttgat ggggtacaac acattgtata
     4501 tttacacaga agatacctat gaagtagaag accttccata ttttggttac cttcgcggag
     4561 cgtatacaaa agaagaagtg aatgaaattg ttgaatttgc ttctggtttt ggcatagaag
     4621 tagttccatc gatccagacc cttgctcact tgacccaatt tttgaagtgg tatgctcaac
     4681 aagaactgat ggatgatcaa aatactctac tgatcggtga agaaaaggtt tatgcggcaa
     4741 tcgaaaagat gattattgcc tgcaaagaaa tgtatcagac agagcgtatt catttgggaa
     4801 tggatgaagc ctatcaagca ggtctgggac gatatttaag cctgtatggg tattgtgatc
     4861 ggacaacttt aatgattcaa catatggacc gtgtgttatc aattgttgat aaatataaga
     4921 tgaaaccaat gatttggagt gatttcattt ataagctatt agatgaaacc ggtaaagata
     4981 ggtattacaa tcctgatttt aaactcaaag cattagcagc taaaggatta ccagcaggat
     5041 taacctatgt tcattgggac tatggttcag atgaagtttc ctcctatgag aaagtaattg
     5101 ataaccattt gaaattttgt gacattgatc attacgtttt agctagtggt gcccatatct
     5161 gggggagact agccccgaat catgggaaaa cagaagcaat tgtttctgca gctgtaaaag
     5221 cttctaaaaa taaaggtgta cgttctgtga tgttaactac ttggggagat gaagggcagg
     5281 aaacagagca ttggcattcc ttaattgggg cattaaaatt agcagattat gtctattctg
     5341 aagaagagtg gaatgatttt gaggaaatct acgatcgttt attaggaaca ggtagttatc
     5401 aattaatgtc cgaattacga tatttcgatg aagtggatac cattacaaat aacaacttac
     5461 aaatggcttc cgtgagtaaa ctgctacttt ggcaagatcc gttagttggt atatatgact
     5521 ttcatgtagc cgctcataac aaaattgcca ctagaacaat tggtacttat tatcatgagt
     5581 tagctcagaa attagatatg aataaaaatt ggaatccaaa acaaccaatt ttaacaacta
     5641 ttcgtcaacg atatatactt ttagctgaaa ttttagcaca aaaggcagaa ctaggcatct
     5701 atttacaaca ctgctgtctg gtagaagacc ttgcaggatt aatggaatta gcgaatcggt
     5761 gtgaagcact agccaatcga gtaaatgaat tggcatctat tcatgaaata atttggtatc
     5821 aaacatacaa aacctatggt tgggaagtat tagaacaacg atatgctgca ttagctcatc
     5881 gtctgaaaac cgttgcgcgt cgtattaaaa atcatcttca ggggcaagat gccctagagg
     5941 agcttttgga agaacgatta ccgttttgtc aatatcaagt gccgcttgaa atttctggct
     6001 ttaattatcg gcaaagctcg gtgtcaggct acaattaacg aaggaatctt cccgttgaac
     6061 ggaccacttg ctcatctaat gggaaagtgg ttcgttttct attcataagt agcttaccac
     6121 taactctttt attaagaaga gaaaaaaagt aggaagtgag aaaatatgtt atcggactat
     6181 tatcaaaatc tgtcagttat aagtgtcaat acagaaccat tacgtaatta ttatattcct
     6241 tatgcaagta aagaagaagc aaaacaagag ttagaaaggg aaaaaacgag ccagtttatc
     6301 gatctcaatg gtacatggga ttttcagtat tatccttctg tccaacagtt accggaaaat
     6361 ttttttcaag aggcctttac tgaaagctat gatagaattt ctgttcctgg agtttggcaa
     6421 acagagggat ttgacttacc tcagtatatc aatgtatctt atccaattcc ttttgatccg
     6481 ccttatgtac ctaatgatac cccttgtggt gtatatcggc gtcagtttca aattgagcaa
     6541 acagatgcac gcttttactt aaatctagaa ggagcagata gtaattgttt tgtttggata
     6601 aatagaaact ttgtaggcta tgaccaagtg agtcatagta catctgaatt tgatgtgacc
     6661 aagtatttac aggttggggt taatgaaatt caaattctag tgatgaagtg gtgtgatggt
     6721 acctactttg aagatcagga taagtggcga atgagcggtc tatttcgtcc agtgtatctt
     6781 cttcaacgac caactatgca tctgacaagt tatcaactac ggacgttgac tgatcctaaa
     6841 aaaggaagcg gaacattaat ggtttctttt caacgattta ctcctattga agtatcgtta
     6901 cctatttctt acgaattatt ccatcctgat ggacgtctca tagcgaaagg ggagtgttac
     6961 tcggaacaac aaataaattt ggatgttgaa gattgccttt tttggacagc agaaacgcct
     7021 aaattatata cattactttt aacatgtaat ggtgaatatt ttaaagaaaa agttggcttt
     7081 cgagcactga cgatcaagaa tcgtaagtta ttgctgaacg gaacacctat taaattatat
     7141 ggagtcaact atcatgacgt gaatcagcaa ggcacgccag tgatgacccg agagcagtta
     7201 cgtgcagatt tgttattaat gaaaaaatta aatgtgaata ctgtacgaac ggcacattac
     7261 cctaaatcac cagattttta tgaattggcg gatgagattg gtctgtatgt aatcaatgaa
     7321 gcagatttgg agtgtcatgg tgtggttaat ctatatgata ctgaagcgaa ttatcaacta
     7381 attgctaatg atccgacatt cgaaaagccg attatggacc gagtgatccg ttctattctt
     7441 ccttatcaaa atcgaagctc tatcattatg tggtctatgg ggaatgaatc tggttatgga
     7501 gtaaattttg aaaaagctca agctttagca cggaaattgg atgattcacg gttgattcat
     7561 aatgaacgct cgattgaacc agttattgac caagtgaatg attttagtaa cttggatgtt
     7621 attagtcaaa tgtatccccc actggaagat gttcatcgtc attgtcaaag aagtgatact
     7681 gaaaaacctt tttttatgtg tgaatatagc cacgctatgg gcaacggtcc tggagatctt
     7741 aaaacgtact tcgatttgat tcaacagtat gattcctttc ttggcggttg tgtctgggag
     7801 tggactgatc atgcaatcaa tagaggaact ttagaaacgc catcttattt atacggtggt
     7861 gattttggtg aaaaatatca cgacggcaat ttttgcgtag atggtttatt gaccccagat
     7921 cggcaattaa cagtaggtgc attggagtac cgtaatattc attcgcctat tcaactgatt
     7981 gctgtagatg aggagctgca agaagtaacc ttgagcaata taactgcttt tacagccggt
     8041 tcagcattta acctttctct tgaatggtat atagatggaa aaaaaacagt ttatgaagta
     8101 atgaatcctt tcatacttga gcctggcaaa agttgttcat tgcccatcac agtacctaca
     8161 acgattcatg gtgctcatac ggttgctgtt tcgctatctg tttataaaaa tgatgagctt
     8221 tatggacagc aacagctctt tcttaaaaaa gaaaaaatga gattgccaag aactgctcat
     8281 cataatgatg caccacaaat ttcagtaaaa ggcacagagc tgataatcaa aggagaatcc
     8341 tttatttata tatatcaatt agcaaaagct gcgttcactt ctgtaaagat caatcaaaag
     8401 gaacttttgg aacgtcctac tcaattttca atttggcgtg caccaacgga taatgatcgt
     8461 aacttagtaa acaaatggaa agcagctgga tatgctgaaa tgtctttaga aatcagaaaa
     8521 aaagactgga aattcgtaga tgatcatctc ctgatcgaaa cgtcttatcg aggagttatt
     8581 ccaggattac agccaatttt tacaatgcac atacagttga aaataaatgg tgcaggcgta
     8641 atcaaccaaa aagtatcagt ggagaaaaat ccgatttttc cagatttccc tcgctttggg
     8701 atggactggc cgttgaatga tactttttcg aaggtttctt attgtggcta tgggccgtat
     8761 gaaagttatc ctgataagca ctatgctgcg atgtttggta tttatgagat ggagattgag
     8821 gaatttgatc agccatatat caaaccacaa gaatatggta accgctggca atgtgattgg
     8881 gtatcaatca aaaatcatca aggatctacc atgacggttt atggaaactc taatttttct
     8941 gctctgcctt atagtcaagc tcaattaacc gaaaagtcac accattatga attaataaaa
     9001 gaaaatagaa cttggttcaa tttaagttat aaacaaaatg gtatcggctc acacagttgc
     9061 ggtccttatt tagaagatcg tttccgtttt atggaagata cttttgactg gaatatagtg
     9121 attactttca aaggagaaaa tagatgaaag aaacaaagat caatcaagtc aaaaaaaaat
     9181 tatctcgtta tcaagaagtt gtttatcgaa accattgctt aaaatggtca aaaaataagc
     9241 tgaatgttcg ttttaatgga ttattttatc gtttcgaaca gttagaagaa gtctggaaac
     9301 cggtaacgaa tttccaatca tgtcctgtac agctcataaa tgatccaaaa gaaacatacg
     9361 atttatgtat aacacacctt tataaccagc atttgaaaaa tgacggattt tatttaaagg
     9421 ttactaatga caatcaactt acgattgaat cagcaaacct tcgagggttt caatatgcta
     9481 tggaagcctt attagagagt tttttttcgg taggagatca attattatta ccggaattga
     9541 ttctgaaaca tgaaccatca gtgaaaattc gaggaatcat tgaagggttt tatggtatcc
     9601 cctggacaca tgaaatgcgc ctctctcttt tttcatatat gaaagataac caattgaata
     9661 cttttatgta tgcccctaaa gatgatgaat tattacgtaa aaaatggcgt gaactctacg
     9721 atgcgcaaga attagacaaa tttaaggagc ttttatctgc tgctgaggct agcaatatcg
     9781 atttttggta cttaattagt cctggaaatg atattgatat tacgtgtgaa gaagacattc
     9841 aagttcttct gaaaaagtta gaacaactga ttgagcttgg cgtatttcaa tttggtctat
     9901 taatggatga tattgactat caattaaaag gtgctgctaa acgccgtttt cgagagccag
     9961 cattcgctca tgcctattta gtgaatcgag tagaggaata tcttagcact cggttgtcca
    10021 atcatgaatt agtcatttgt cctacagaat atgataatcg tcatggttct cgctatttag
    10081 caacgttaag tcaagaaata ccagaacaat taccgttctt ttggactggc ccaagcacgt
    10141 tagcggcgtc aatttctaca gaagagctgc aggaaatggc ttctgtctat caacggccga
    10201 tgctgatttg ggataatatt ccggtaaatg attatttaga ggataaagaa ctattgttca
    10261 tgagtccata tgagaatcga acaccgaatc tttctaagga gagataccag gttacaggag
    10321 tagtttcgaa tccaatggct caattagaag cttcaaagtt tacgatcaat agtatggcta
    10381 attacttatg gaactgtgag cgttttgatc ctttggaaac ttggacgtca gtcgttgaaa
    10441 aagtagttgg aagtaaacta caaccagctt atcttacctt aaccaatgct tttccaaatc
    10501 attacacaag cagtctgctt tcagatgaag aattactgct agccaaagac ccagaatggg
    10561 tcacaaaaga aatggctgct ttagtccaag cagtaaaatg gatccagcaa gaacaacata
    10621 catctagatt gagaacagaa ttggagcctt ggttacaagc aatcacagaa agttggactt
    10681 tctggcaaga atggcaatta aaaaaagatc caaaagaagc agaagaatgg ttggctaaga
    10741 aaaaaacaca ccgcatagga cgtgatttag taactgccca tttagagcaa attatcaaaa
    10801 gttagatatc ttaaaaaata aaaagcaaga aaaaggagaa caaaatggtc aagagattaa
    10861 ttagtgcaaa ctacagtgat gtcaagaaaa tgacgtcaaa agagttgaag caatccatca
    10921 aagcgagtga aggacgggtg attgtttgtg aaacggttgc taatactctt ccacaatctg
    10981 gagaaattac gaatgcagaa gttgcgtcag catttggtgc agatttgatt cttttaaatg
    11041 gtttcgattg ttatcgtcct gcagtttttg gactaccaaa tgtgacgatt gagaaggttt
    11101 ttgatctaca gcaaacgatt gaaaacccga ttcctaaatt aaaagaacta gtcggtcgtc
    11161 ctgtgggaat taatttagaa cccattgccc ctaaaacaaa tatgatgagt gagaaagtag
    11221 aaatcagcca aggaagagca agctctgttg aaacgataca aaaagcagaa gaactaggag
    11281 tcgattttat ttgctttacc ggtaatccag gcactggagt aaccaattta gaaattacta
    11341 aatccgtaaa aatggcaaaa aagtattttt cgggattgat tattgctggg aaaatgcata
    11401 gcgccggctc agaagagcca gttgtgaata aagaggcgat tatttcttat gcggaagcag
    11461 gcgcagatat tttactagtt ccttcagtag ggtctatccc aggcttttta gagcaagacc
    11521 taattgaagc ggttcggttg gcaaaaaaat atgatttgtt aacgatgtct gcaatcggta
    11581 ctagtcagga aagtgcccga ccagaaacat tgcggcaatt agccattatg aataaaagtt
    11641 gtggtgttga tttacaacat attggagatg ctggatacgg gggattggca ccagcagaaa
    11701 atattttcga aacctcggtt gcaattagag gaatgcgaca taccatcaac tgcgttagcc
    11761 gctcgatcaa tcgctaacaa aattaaataa aaaatggtta aattgattat ttaagtggga
    11821 acatacttga gatatcagct aacttctgtc actttaaaaa agggactgtg acagaagtga
    11881 aaatagacaa aaaatatccg aacaagagac taggatagca gaaaaactat cgttttgcaa
    11941 cgaggaccaa ctggaccgta ggagcaattc tcttattgtt cggatatttc gcttgttcaa
    12001 ggacttatgt ctgtagtgcc cttttttggt aggaaaaatg gtagggaacc atatcatttt
    12061 taatgaaaat caagcatttc aaaagttgaa a
//
LOCUS       DAZKCY010000074        12059 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000074 DAZKCY010000000
VERSION     DAZKCY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.075"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACG8XC_001888"
     CDS             4..2376
                     /locus_tag="ACG8XC_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9191580.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNDQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQVPEKEQKQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGHTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQD
                     EKNVVETAEQKNIVLVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDTQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACG8XC_001889"
     CDS             2437..3897
                     /locus_tag="ACG8XC_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP9191581.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVQDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEMKVEPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGKQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACG8XC_001890"
                     /pseudo
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACG8XC_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            6128..6277
                     /locus_tag="ACG8XC_001891"
     CDS             6128..6277
                     /locus_tag="ACG8XC_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191582.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6358..6954
                     /locus_tag="ACG8XC_001892"
     CDS             6358..6954
                     /locus_tag="ACG8XC_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFP9191583.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDTERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6967..7866
                     /locus_tag="ACG8XC_001893"
     CDS             6967..7866
                     /locus_tag="ACG8XC_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFP9191584.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKEQKLAYQRHQQMNREMER"
     gene            7869..8353
                     /gene="ssb"
                     /locus_tag="ACG8XC_001894"
                     /pseudo
     CDS             7869..8353
                     /gene="ssb"
                     /locus_tag="ACG8XC_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            8375..8632
                     /locus_tag="ACG8XC_001895"
     CDS             8375..8632
                     /locus_tag="ACG8XC_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191585.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8791..8925)
                     /locus_tag="ACG8XC_001896"
     CDS             complement(8791..8925)
                     /locus_tag="ACG8XC_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFP9191586.1"
                     /translation="MLKSIKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK
                     "
     gene            9068..9328
                     /locus_tag="ACG8XC_001897"
     CDS             9068..9328
                     /locus_tag="ACG8XC_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191587.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            9777..9983
                     /locus_tag="ACG8XC_001898"
     CDS             9777..9983
                     /locus_tag="ACG8XC_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFP9191588.1"
                     /translation="MRKKFDYLGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIEEKGKKR"
     gene            9983..10234
                     /locus_tag="ACG8XC_001899"
     CDS             9983..10234
                     /locus_tag="ACG8XC_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191589.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGCELHYQENLETYENEYWLTKLEN"
     gene            10250..10687
                     /locus_tag="ACG8XC_001900"
     CDS             10250..10687
                     /locus_tag="ACG8XC_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191590.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCFEIGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            complement(10798..11025)
                     /locus_tag="ACG8XC_001901"
     CDS             complement(10798..11025)
                     /locus_tag="ACG8XC_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191591.1"
                     /translation="MKQNTSITKNTARFNCTAALSRISISPTNSTNNKGIIFFKWITF
                     FLLSYRALPVYFAPPKRRGRLVRNKNHSLSY"
     gene            11123..11311
                     /locus_tag="ACG8XC_001902"
     CDS             11123..11311
                     /locus_tag="ACG8XC_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191592.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IACIGQYLYCIRGRKLYA"
     gene            11429..11635
                     /locus_tag="ACG8XC_001903"
     CDS             11429..11635
                     /locus_tag="ACG8XC_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191593.1"
                     /translation="MKYNLLDHKRMWVEKLEEDLEQLELLGHNKTSTVYKEGLKRTKK
                     AQKELATLESRVQKKEVDLTHTYL"
     gene            11645..11878
                     /locus_tag="ACG8XC_001904"
     CDS             11645..11878
                     /locus_tag="ACG8XC_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFP9191594.1"
                     /translation="MADKRKEKIQKLLALAADVNDEESVSALAKAQQLMMDYDLTEDE
                     IFNYHEQQRIEAVETHVIYRGRPQKWLYRLFRN"
BASE COUNT         4458 a         2028 c         2543 g         3030 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaatgat caaatcgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaacgggc attcttggtt taggcaacaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gttataacaa gtacggtggc
      301 ctggatatga cgatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc tgccacgatc cgaaaatacc aacgaaaatt ggaaacatta
      421 gaacagctga aagaaaaatc agagaaagga aaagaaaata ttcttcctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaagaatc caactatcta ttttctgaaa
      541 ggctatcgaa aagtcgcttt agaactcaat gaaaacgggg gatttgagga atctaaaaaa
      601 taccaagcca cgtcggaaga agaaaaggca gctgttcgtg aattacttga agaagctaca
      661 cctactgatt ctgtgaagga gaaagaaacc gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag caaaagggtt gaaaagttat ggtggggaga tcgtaacact agaattgatc
      781 gatcaactca aaacactgga taaccaatac gccgcagaag caggctatta taaattctat
      841 tttgaagaag tgaagaatgg agaagtagta gatcagatac gaatggatct aggggatggc
      901 gtcaatagta atcaccccat ctatttagag ttagctagat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaaacagt caaaaatgaa cctatttcaa aagtacaaaa tgagattaaa
     1021 gagacaaaaa caaaacaaaa gggttatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatcac tttcccaaca ggcgcttgat gcggtgaaaa agcatactca ttcacctgaa
     1141 gacgtcaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgatcg aggaacaatt tccaggcgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaggc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacaa ttgaagcctg aagactatcc gaaaatcttc
     1501 ccgaaccgcg tctttgattt tcaattggat caaacagggc atacagaact tcaagttggg
     1561 attcaagcgg tagccaaaaa tattggaatt caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtacgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagccac aagtattcac
     1741 gaattagccc atgctagaat gcacaaattt tcagaattag atactgcaac aaaagaacta
     1801 caggcagaaa tgacctccta tattgtgtgc aagcattttg ggatggacac ctcggagaaa
     1861 gctattcctt atattgcgac atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agtcgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccagcc agcagaagtg
     2041 aagcaggatg agaaaaatgt tgtagaaaca gcagaacaaa agaatattgt gctcgttggc
     2101 tcactttgga tcgacaagaa agacgggaaa gtcctggaag aagacacggg ccgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat acgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gattcaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatttg
     2821 aaggaagaac gaaaactgcc cgattggttg attgattatg cagaaaaaga aggattgatc
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcaagacga tcgtttggat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacat tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggatttaac ctaaagatcg gtgaccctaa acagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccattga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtt gcaaaagaaa tgaaagttga acctgaagca
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aaggttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggattg gaatgatcaa ttgaagcatg aaatccacca acaagagatc
     3841 agaaagcaaa aacgtgcgat gttgtttcaa caaggcaaac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccaccc gaaaagacgg gtattttgaa atccaagata gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gagttagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agagcatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatagcttg
     4261 gacgatcatt caacaagcag gcgccgatca tggaaagata tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcagtg attccagcaa ttacgagacg cagaagagac
     4381 ctatcccaaa tttgaagaag cacaagcacg ccagatcgct gattggttga tcggcatgaa
     4441 cggcagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcag ctgcaggaaa agattagaaa
     4561 cttccaaaaa gagccttatt ttgaaggaaa agcccaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggtacg atccaccaag ttgaaaccga
     4741 gaaaaaaagt gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagatac tttggaagcc atacaagggt tgtatgaagg
     4861 gaaatatcta agctacccac gaacagatac cccttatatt acagaagggg agtatgcgta
     4921 tctgttggat cacttagacg aatacaaaca ctttttaaag gccgaagcca ttcctactcc
     4981 tattcataca ccgaatagtc gatatgtgaa taataagaaa gtccaggaac attacgcgat
     5041 cattcctaca aaaacggtca tgacggcagc ggcttttgaa caattaagtc cactgcagca
     5101 ggccatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagttca gcagcttcag ttaaaggcaa tcggaaaggt
     5221 gccgctagat ctaggttgga agaagttatt tggaaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtgg atctacaagt
     5341 tcttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcaat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agctcgtggt gacgaataaa gggattttgc tttgccaagc
     5581 cgtggaaaaa gagccgctgc ttacgagcgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatc ggtgaacgaa aaggaacaca aaccactttt ctagccaata tccaaaaatt
     5701 tgtctctcac ttattggaag tcgtaccagg gcaaatccaa tccactgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttgct ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatc attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcattt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgccgc actgcaaaat aatggagggt
     6121 taatcttatg cgtaatacca cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caggaaaaaa tgcgtgccag caaacgaaga ctgcagcaat tgaagaacca
     6241 gcagaggatt caaaaagaaa tcgaattaga tcgataaaat cttcctaact attgtatacg
     6301 tataaactta tgttatactg tttttataag tttatacgta tacaaaggag ggagaaaatg
     6361 acaaaagcaa tggtatacaa gctgctagaa gaatttaatg gaactttggc tttgaaggat
     6421 gccaaaaaag cgggcatttc tcctgtaacg atcaaacgta tggtagatcg aggggagctg
     6481 gatcgagagt atccaggttt tttcacctta cctggtcaat ttcctgatga attgtttatg
     6541 gcgcaaaaaa aatatgaacg cggcattatt tcacatatta ccgcgttaga tttgtatgat
     6601 ttaacagata tgatccctag acagatcgat ctgactgtac cttacggtta tcacgtttct
     6661 gaaaaaggat tgaaggaatt cgcagttgag ctacactata caaaagcgga atggtatgaa
     6721 ttaggtaaga ttgaaataaa aagtcgctat gggaatccta ttatagctta tgatacagaa
     6781 agaactttat gtgacatttg gaatccctgg tataccgttg aggatgaaat caaagtaaaa
     6841 gcaatcaaaa actatatgga gtcggacaga aaaaatctta gaaaattgaa tgagtatcga
     6901 cgaattttac caacagacaa aacgatgcgt tcttatatta tggctttaaa ttaaaggaga
     6961 agatacatga cacctacgca gttaaaagca aaagtgaaga atatttctag agaaaaagaa
     7021 gttgatccgc agcttgtttt aagacatttt atgatggaaa aattcttgga aaaaatctca
     7081 gagtctcctt atcgtgaaaa ttttgtgtta aaaggcggct tcttgattgg ctcaaagtat
     7141 ggcattgaaa atcgcacaac caaagatatt gatacgactt tacgggaaat gaaagtaacc
     7201 aaagaaacat taacaacggt tctaaacgaa attttttcaa cgccgacgaa agaaggcatt
     7261 caatttgaga ttcaaggaat gaaagaaacg agagaagccg attactatcc aggatttagc
     7321 ttacgtgtgc tggcacattt ggaaaacatg cggccagatt ttaaggtaga tgtgactaca
     7381 ggtgactcga tctatcctgc aacgatcact cacagtcata aattgatgtt tgaggatcga
     7441 acgattcctt tggaatccta tcctacagaa caaattattg ctgaaaaatt aagtgcaacc
     7501 tttgattttt taacagataa ttcgagagga aaagattttt atgatctcta tactattcct
     7561 aaaatggaga agatcgatga gaagaccttg aaagatagtg tgagaaatac atttacacgt
     7621 cgaggaaaaa ctgatccgtt aaagatatac tatgaaaagg atatggatgt actgaaaaat
     7681 agtcacgagc taaaagatac ctggaagaaa tatcaaaaaa gcaatcctta tgctgcatcg
     7741 atcacgtacg atgagacaat ggattcgatt agtaatttga tggaaaaagt gattcacgtc
     7801 gaagaaaaag agcaaaaact agcttatcaa agacaccaac agatgaatag agaaatggag
     7861 agataaagat gattaataat gtgacattag taggacgctt aaccaaagat cctgatttac
     7921 gttataccgc aagtggtact gcagtagcga cttttaccct agccgtcaac cgaaatttca
     7981 cgaatcaaaa cggcaatcga gaagccgatt ttattaactg cgtgatttgg cgcaaaccag
     8041 cagaaacaat ggcgacatta gctaaaaaag gtagcttgat tggtgtagtt gggcggattc
     8101 aaacgcggac ttacgacaac cagcaaggac aacgtgtata tgtgactgaa gttgtagcag
     8161 ataactttca actattagaa aataaagcag cgacagagag ccgtgcgcac gctgatcaat
     8221 cgtccaccag tccaagcacc gccacctttg aacaacggat acagcgacgc ctaacaacaa
     8281 tggattgaac gcttcacaga atccatttgg tggacaatca atcgatatta gcgatgatga
     8341 tctaccgttt tagtttgaga aagaaggagt aagcatggca aaaaaaatta gtctgacgat
     8401 ggatctattg atacccgtaa ttgcatttat agacgggatt tctttgggcg tcactgaaaa
     8461 tcctttggta aatagaattg cgaaaggatc actatttctt gtattcgtgt atcttgtctg
     8521 ttcttatctt ttaaaaagag caggaaagga taattccttt tggcgattga ctaaagtcac
     8581 agtagcagcg acagctgtta tacttacggc tattctaccg ctttatatgt agagttaacg
     8641 ggaaatcgtt gaacttcttg cgaaatattg ctataatgaa catgaaaaag aggagttgcg
     8701 ctaacaaccc ctcattaaca gcaccgatta aagaacggtg gcagaatatt agttatttgg
     8761 aaataaccgc tgacctgcta aagtagacgg ttatttcttt ttgtcatttt taagcatatt
     8821 tacaatcaaa ctaatcaaag caatggtaaa cataccaaaa ccaagaatgg tttgaattgt
     8881 ctcatatgcg gacaaaaggc ttactccttt tatagatttt agcacttaca ttcataagca
     8941 ccaccacctt tccagaagtc gccaccgtct tcaaacttta ctgtcttgac tattatagca
     9001 tatctaaaat cgaataaaaa ttctcaattt taactgtttt tgctataatt ttagcgaggt
     9061 gatcgtgatg aacaaatatg ttggtttgct tgataaaatc cgagtaataa aaacacaacc
     9121 attattggtt cgctttactt tacaaacaat tcatgaatcg attaattgtg tagttgcaga
     9181 tattgaaatt atcgataaat tgttaattat ggatgatgga aaatacaata ttgccgtcac
     9241 aggtcatttc aataaacgga atcaattagt cattgaatcg atgcaaatta gaaaccctga
     9301 tcattttacg cgatcaatgg gcatataaat aaaagaagag ctgcaatcct aatgtaggac
     9361 tgcagctctt tttgttttac atagaaacag gaagaaaggg aaaggcctgt ttaggaaggc
     9421 acaagtgcct tctcaattga aaaacctttc tctcactcca accgccaccg ccgttcgaga
     9481 agaaaggaag tgaacgttgc acgttcacta agataagggg tcttgctttg taagacaaaa
     9541 taggaaaagc ttcctctcac caaaaggttc gttcctgttc gctatctctt tattgatttg
     9601 ccagaccggc aacaagttgc cttttatcac caaaaagcga caaggacaag ggtaaatggc
     9661 acgctgcgcg cccttgtcct tgtcgctttt cgttgattca ttctggccaa caaatcaata
     9721 aagaaagaac taacgaaagg aagtaaaacc tttcctaaga ggaggcagaa tcaatcatgc
     9781 gaaagaaatt tgattatttg ggtgttccct tcagcgaatt attcccaaac tatcatgcac
     9841 cacatactgt cgaatgcgat tgtggagaaa gagcgaaatg tatcaaaagc tattgtttgt
     9901 atcaatgtcc aacatgcggg aaaaaatata ccttgtctta tggcgattat attttgattg
     9961 aggagaaagg aaagaaacgc tgatgaaaac acaacacaaa ttattgactg taaaagaagt
    10021 cgttcaaatt ttggtggaca aagaagtcat ttctgaaaaa gtagcagaag ctttacttat
    10081 tccgcggtac tggacgatag aaaccccttt agtagaggat gcaccgacta tgctatttga
    10141 agacctagtg aagattgcag gatgtgaact gcattatcaa gaaaacttgg aaacttatga
    10201 aaacgaatat tggttaacca aattagagaa ctaaaaagga gaaaaaaata tggctcaaaa
    10261 ttactatgac gaatttgtca aattaccttt ggataaaatg gcacaaaaaa tggaggatat
    10321 gacgttccta tacaacgaaa cacgtgttcc aaaaaaacat tataaagaaa aattgtcggt
    10381 agctgtggaa gaaatgattg aatcaggtgt cgaaatgaat cttattgcga cgtactatcg
    10441 aacactggaa gaactgaaaa agcaaaatgc aaaatggttt ttccaagcct tactatgctt
    10501 tgaaataggt gtcaaaccaa gcactattaa accttctgaa taccaggctt tagaactaac
    10561 gtatgcgaaa tttatcgaaa cgaaaaaagc caagactgtt tcaagtgaat ggttagatta
    10621 ttttgaaaat atcaataagt atggcgcata ttacaccatg aaaaaggaag acaacgaaaa
    10681 tgaataaggt gatttatcaa ctaacaacgg attccatatc aacgatttga aagaaaaaaa
    10741 ggtcaataat catttatacc gaaaagtgag aatgtgtgga ataaggaaaa aaaaagccta
    10801 gtaactaagg gaatggttct tatttcgcac caaccgcccc cgccgcttag gtggtgcgaa
    10861 ataaacgggc aaagcccgat aacttaagag aaagaaggtg atccatttga aaaagattat
    10921 tcccttattg ttagtactgt ttgttggcga aatacttatg cgagatagtg cagctgtaca
    10981 gttaaacctc gctgtatttt tggtaatact ggtgttttgt ttgatctttt ggcggtttgt
    11041 attagtcctc gtgagtctac tattcaagat gattgcagcc atatttttca tggggttatt
    11101 tcgatgaaag gagagagtac caatgccaat tgcaatagga aataaacggt tacccgtgac
    11161 attagatgaa aagagacaaa aagaattgca gcaactaaag cagaagtacg gcaaaagtga
    11221 atccaggatt atgtgtattg cgttagattt attgattgcc tgtattggac aatatctcta
    11281 ttgtattcga ggaagaaagc tttacgcttg aatgcgataa gttaacccga tatatcgatc
    11341 caaaattcta tgaggataaa aacctggcat gggaactggc tttatagaca gagtgctagt
    11401 gcaagtaaag gaagaaagga agatcaccat gaaatacaat ttattagacc ataagcgaat
    11461 gtgggtagag aaattagagg aagacttaga acagttggaa ttgttaggtc ataataagac
    11521 ttcaacggtg tataaagaag gactaaaacg aacgaaaaaa gcacaaaaag aactagcaac
    11581 actggaaagt agggttcaga aaaaagaagt agacttaaca cacacttacc tttaggagtg
    11641 gaaaatggca gacaagagaa aagaaaagat tcaaaaatta ctggcgcttg cggcggatgt
    11701 aaatgatgag gaatcagtga gcgcattagc caaagcccag caattgatga tggactatga
    11761 tcttactgaa gatgagattt tcaattatca tgagcagcaa cggatagaag cagtagaaac
    11821 gcacgtgatc tatcgtggac gtccgcaaaa atggctgtat cgtttattta gaaattaaga
    11881 taaaaaaggc tagttaacag gtttgttgac tggccttttt tgatatgttt gtacatgttt
    11941 tattgtgttt taatttttct cttttttata gcaagctcct tttataataa atattccgaa
    12001 aagtgaagac cattttttat ttattaagac tattattatt agtgagataa aatttgatt
//
LOCUS       DAZKCY010000075        11757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000075 DAZKCY010000000
VERSION     DAZKCY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.076"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..877
                     /gene="thiD"
                     /locus_tag="ACG8XC_001905"
     CDS             62..877
                     /gene="thiD"
                     /locus_tag="ACG8XC_001905"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP9191595.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1206..2657)
                     /gene="cls"
                     /locus_tag="ACG8XC_001906"
     CDS             complement(1206..2657)
                     /gene="cls"
                     /locus_tag="ACG8XC_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9191596.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            2998..5664
                     /locus_tag="ACG8XC_001907"
     CDS             2998..5664
                     /locus_tag="ACG8XC_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9191597.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIAFGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6028..6780
                     /locus_tag="ACG8XC_001908"
     CDS             6028..6780
                     /locus_tag="ACG8XC_001908"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9191598.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6802..7956)
                     /locus_tag="ACG8XC_001909"
     CDS             complement(6802..7956)
                     /locus_tag="ACG8XC_001909"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFP9191599.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8141..10582
                     /locus_tag="ACG8XC_001910"
     CDS             8141..10582
                     /locus_tag="ACG8XC_001910"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFP9191600.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(10912..11616)
                     /locus_tag="ACG8XC_001911"
     CDS             complement(10912..11616)
                     /locus_tag="ACG8XC_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191601.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         3787 a         2098 c         2489 g         3383 t
ORIGIN      
        1 atgatagaaa cagtaacagt attatgaaaa atctttttat aaaaatggag gaatcgattt
       61 gatggaagat cagtatgtac aagcaatgac gatcgcagga tcagatagcg atggaagcgc
      121 agggatgcaa gcagatatgc atacattctt tacaagaaat gtttacggtg tttctgtcat
      181 gaccgcatgt gttgcaggta attcttacgg aatcggagca agtgtgacat tgccaacaga
      241 ttttatcgac aaagaatttg aactgattgc gaaggacttt caaatacgtg cagctaaaac
      301 aggtatgcta gcagattcaa agttgattga gacagttgta aaaaattacc aaaaatatga
      361 ttttggacca ttagtcgtgg atcccgtaat cgtaacgaaa catggaaatc ttttactaga
      421 agaatctgct ttgcaaagct tgaaagaaaa gcttgtccct ttggccgaag tactgacacc
      481 taatttttat gaagcagaaa agctgactgg acggacatta gcagctgaag aagattttgt
      541 gcaagcagca aaagaactgc aagatatggg tgcaaaaaac gttatgatca aaggacatca
      601 tgctaaaaat gcgcagcaag aaacagtcaa tgactacgta ttgttcgaat caggaaaatc
      661 attttggtta aatgcaccat tttatcctac cgaccggatc aacggaactg gcgattcgct
      721 atctgcatgt attgcagctg agttagcgaa aggaaaaaca gttgaacaag cgatccgatt
      781 ggcaaaagac tatgtaaacc gtgcaattcg tgaagaaata aatgtagggc acaaatatgg
      841 ccccatcaat cattgggcag ctggtgaatt agagtaaaag aaagaagttg tgaaaaagtt
      901 gccctgcatc aagtaataag aacagccaag taaaaacagc ttcttatgtt tttaacttgg
      961 ttggacttat taccgcagat gcaagctttt gaacaccgtt taagaaagag gtcgtgagcc
     1021 tgccgtccag ctccgagtca caaggaacaa gttcaaaaaa cagcttctca tgtttgttga
     1081 atattgtgac ttgtgaccga ggagttggca ggagaacacc gtttgttcgg aagaagggac
     1141 tgtgacaaga tttttgtcac agtcccttta atcttagtat atttttctct acttgaataa
     1201 aaaaattata ggattggaga aaataatcga gaaatttgtt gcttgaataa tagccagtta
     1261 gacatttcct tgactgtttc agttgtcatg gatgaacaat gtgtctcatc ttctttgaac
     1321 tgagtagcaa gttcttctaa aaactcagga tcgtaaatga cagcacttgt ttcaaaattc
     1381 aatcggtagc tacgaatatc ttgatttgct gaacctacca tgcagatttc atcatccatt
     1441 atcaatgttt ttgcatggag gaagccgttc tcatagacaa ggatttgtac attttccttg
     1501 atcagctgcc gagcataata ttgtgttgct cgataaataa aaggatgatc cggtttgttg
     1561 ggaatcatga ttttcacatc tacaccagag gcagttgcga tttttaaagc agcaatgaca
     1621 ctttcatcag gaacaaggta aggtgtctgt atccaaacac gttttttagc agaagtaatc
     1681 aatttgataa atgaaagctt gatttgttcc ctttcgttat tcggtccgct tgaaacaagc
     1741 tggatggatg tatcagcaag cttagattca tctcgatctg cttttttaaa gaaataattc
     1801 aaatgatagc cgaccttttt ttcttcaggt accgagacgt tccagtctgt taagaagcga
     1861 agctggagca aagaagaagc ggcaccgaaa atccgtatat gcgtatcgcg ccaatagccg
     1921 aatttttttg ttgtatttac atattgattg gcaatattga aaccaccggt ataactaatc
     1981 tttccatcga tcacaacgat ttttcggtga tcatggtaat tcaatcggaa acgaagtaat
     2041 gccctttgtg aggtaacaaa tgtatggaca tgtccaccat ttttgattag acgattgaaa
     2101 tcttttgctt ttgtgccatg agagccaaat gcatcatata atattcgaac ttccacgcct
     2161 tctgcggctt tttcttctaa aacatgtaag acttgctggc cgatattatc tgtcacaaat
     2221 gcgtaatatt cgatatggat cgaatgtttg gcttttttta gatcttgaag cagtgaatcc
     2281 aatttctctt gtccgtctgt gagaagagtg acagaattca ttcttgtcag cggcatacga
     2341 tttaatgaag agaagaagtc aacaaattgc tgtttgtctt tatcgcccat gtcaggatcg
     2401 taatgttcaa tggtatctcc tctaaaagac tgaaagtttt ctaattcttt caagtcactt
     2461 tgtcggagat agaatttttt cttatccgtt aatccacgtc cgaaaaataa atataataca
     2521 aagcccaccc caggaagggc aaacaatact aagagccatg cccaaatggc tgctacatct
     2581 cggggtttga tcaaaatagc caccaaagca ataagtgcat ttaatagata aagggcggtt
     2641 ataatactag ataccacaaa aatgttcctc cttacataca ataacattag tatagcatga
     2701 tagaagaaag aagcctacca aggtgaatat acttttcact cctttcatga aaatgtaggt
     2761 aaatcgggct aaagtagttg ctttttgtag gttaataagg taggatatta gtacagttga
     2821 atatctcctt aggggagtaa ccggcaacgt ttgttgcgat aatatcaaca gtaagttaac
     2881 caaatggttt tctggtattg tatgaaatgg tgagacctaa gacaaatgcg tatttgtctt
     2941 aggtctcttt tttttcagac aggactaggg ccaagatcaa aaaatggggg atgaaatttg
     3001 gaaaacaagg aacaagcaca acaagcggtc tataaagaaa cgatcgactc gttgatgcag
     3061 caaacgcaga gctcaaccga tggattgagc accacgcaag cgaaggaaaa attagaaaga
     3121 gatggcttga accagctaaa agaaaagccg aagaagccaa cttggaaatt gttcttagaa
     3181 actttcaaag atgcaatggt tatcgtattg ctgattgtcg cactgattca gatggtcatg
     3241 ggtgactaca tcgaattctt agttattttt gcagtattga tgttgaattc tataatcagt
     3301 gttgtccaaa caaaaaaagc agaaggttct ttagaagcgt tgaaaaacct ttcagcgccc
     3361 gatgctcgag tgattcgtga tggtaaggaa aaaacgattc cggcaaacga acttgtggta
     3421 ggagatatcg tgttgttaga cgcaggcgat tatgtacctg ctgatggacg tttactagat
     3481 gcgggatcat taaaagtaga tgaaggtatg ctgacaggag agtcgacttc tgcagaaaaa
     3541 gaagtaacgg atatcacaag tacggtacca atcggagatc gtacaaatat ggtattcagt
     3601 ggaacgatcg taacttacgg acgcggtaaa ttcttagtga cttcaactgg gaataatacg
     3661 gagatcggac aagttgctgg ccttttggaa tcaacaactg agcaagtcac tccgttacaa
     3721 cgaggtctgg ataaattcag taaaaaacta agtttggcta ttttggcatt gtctttattg
     3781 attttaggta tccaactgtt tcgtatttat cttggagaag gtacaggaga tatgactgca
     3841 gatatcgtca gcgcagtgat gtttgccgta gccgtagccg tagctgctat tccagaagca
     3901 ttacagtcta tcgtaacaat cgtgttatct ttgggaacga ataaaatggc aaaacgtcat
     3961 gccattattc ggaagcttcc agcagttgaa acacttggtt cgacaagtat catctgtaca
     4021 gataagactg gtacattgac acaaaacaaa atgacagttg ttgactactt tttaggtaat
     4081 ggcgatactg gggatttcac aaatgaccct tcaaaatgga cagctgacga acgtcgcttg
     4141 atcgaaatca gtgttttagc gaatgatgca tcgatcagtg aagacggtac taaattgggc
     4201 gaccctacgg aagtagcgtt tatcgacttt agtgaaaaat taaaccagcc ttatcaggaa
     4261 atccgaaaca actacccaag acaagcagaa cttccttttg actcagatcg aaaattaatg
     4321 tctactggac acacaattga taacaaaaat tacttgtttg ttaaaggcgg accagatatc
     4381 gtcttccaac gcagtaaaaa agtattgcta aatggtgaag tcgttccgat gacggatgaa
     4441 ttgatgaaaa aattccaaga gcaaaatgaa gctttttcaa cacgcgcatt acgtgtacta
     4501 gcttttgctt accgtccact tgataatttt gatttatcat ttgaagatga agacgatttg
     4561 atccttgttg gtttgcttgc tatgatcgat cctccacgtg ctgaagtgac tcaggcggta
     4621 acagaagcaa gaagtgcagg aatcaaaaca atcatgatta ctggtgacca caaaacaaca
     4681 gctgttgcta tcgcaaaaca aatcgggatt tttgaagaag gaaacctggc tttgaccggg
     4741 acggaactag atgaattgac agaagatcaa ctaaacgaag ttttagaaaa agtcactgtt
     4801 tatgcacgtg tttcacctga gaataaaatt cgtatcgttc gtgcttggca aaataaaggt
     4861 catatctcag ctatgacagg tgatggtgta aatgacgcac cagcattgaa acaagcagac
     4921 atcggaattg ctatgggaac gggtacagat gtggcaaaag atgcagcagc gatggtattg
     4981 acggatgata actttgcttc tattgtcagc gcagtagaag taggacgtaa tgtgtttgat
     5041 aatatcaaga aagctatttc ttatctattt gcaggaaatt taggtgcgat cattgcgatc
     5101 atctttgcct tagttgctga ctggtccaat ccatttacag cattacaact gttgttcatc
     5161 aatttagtca atgattcact gccagcaatt gcattcggta cagaaaaagc tgaacctgct
     5221 acgatgaacc gcccgccaag agatccaaat tcaggaatct ttacggacaa aacattgatt
     5281 tctgtaacgt atcgtggtgt gctgattggg attgctgtta tctttgctca atggcttggc
     5341 atgcagcatt cacctgaatt cggtgtagcc atgtcatttt caacattgat ttggtgtcgg
     5401 acattgcaaa cattcccagc acgttcaaac acacaaacag ctatccaagc agggttcttc
     5461 tctaacagaa gtgtcttact agctgtagtt gtatgtagcg cgctttactg cttaacaatg
     5521 atcccaggac tgaaagaagt cttctctatc ccatcagcat ttggattgac agaagtcttt
     5581 acatgtatgg gaatcgcact tgtgacaatt gttttgatgg aaatcactaa attgatcatt
     5641 gtacgagtac aaggaaataa ataaaacgaa tcaacgaggt tgtgagcctg ccgtttaatt
     5701 ctgagtcaca aggtgcaatt tcaacaaaca gcttctcatg tttgttgaat attgtgactt
     5761 gtgaccgaag agttggcagg agaacaccgt ttaaggaaga agttgtaaaa tcagcatttt
     5821 gagctaatac tgagggtcat ctgattggac actgtttatt cggaaagagg gactttgaca
     5881 cgactttggt cacagtccct ttttttattt atgatagacg gaaagccaat cattttatta
     5941 ttcatcaaaa gagatagaat agataaaaga ggagaatgat ttattccgaa aagtgaaaac
     6001 atataacaaa aaattagagg aaatcaaatg aatgaaaaag aatgggatga atttgctgaa
     6061 gaatattatg aaatccagca agaatcgcaa gtaactattg ctgaagatgt aaaaaattat
     6121 ctgaaaacgg agaaagtcct gcctgctgaa acagcagcag acgttgctgg aggatctggc
     6181 agatttcttt ccttagcaaa agaaattaat cgctatgatt tgatcgattt ttcttcagga
     6241 atgctagaaa atgcaaaaaa agaagcagaa aaaaatgagt tgccaaatgt ttcctttatc
     6301 aaacagtcgt tagaagaatt tatccaatca aatgagacat atgaaatggt ttttacagca
     6361 gcaaatccag cattggtaca tcttattcaa ttagaaaaat tgatgaaaaa gtcaagcaaa
     6421 tggtgcgtga taatcagagt agtggaatca atcgatgatg ttttttatcc tatcgaaaag
     6481 caactaggaa tgatagaaga agatcctaat acatcgccag ttgtaatgga tcaatttgaa
     6541 acatacttaa ttgatgaagg attcaaagtg aagaaaaaag attttactta ttttacacag
     6601 gaatcaatta ctgaagaatt gatgaagcta tactatgacg cgtacgcaaa agatcctgtt
     6661 gtgaccaggt acattcgcaa actattcagt gaaaaacgag aaatccttag tacgactaag
     6721 ctgacctata gactgctcct ttggtccgca gataaaaaaa gaacctttca cgactcgtga
     6781 agggttcttt ttatgatttt attatatttt gtagtaacga atatcatttt cgatctttgt
     6841 aatcgaaaaa ggtacatctg aagtatattc tgcacattct ccgtgttcag gaagcgtctc
     6901 actgaaaatc gtttcatatt cgtcaacagt gatccgtgtt cgagaatcta gcatctcttg
     6961 atgagattgt gcgaagagat attcttgata gttttcttcc agataacctg taaaaaattc
     7021 acttactgca ccagaaccgt agctgaatag accgatgcga tctcctggct gtagtgattt
     7081 cgaattttcc agtagagaag ttagccccag gtataatgaa ccagtgtaaa gattaccgat
     7141 tcgtcggctg taacggatgc tttcttcata gcgagccata agacgttcct gattttcttc
     7201 gtctgtttgg tctaatacgc tttgcaatgc cttttttccc atcttagtat acggaatatg
     7261 gaaagcaatc gcctgataat cttcgagtcc tcgacccgac aattctttat gtcgattcca
     7321 aactttttgg aatgattcga tatacgtaga attagataaa ggaccatcaa caacaggaaa
     7381 ttcgctataa tctggacgcc agaaatcata gatatcttct gtcagaaata cgctgtcgtc
     7441 ttcaatcgat aaaatacgcg ggttttgagt gatcatcatc gcaacagcac cgacaccttg
     7501 cgtcacttca ccaccgcttg ccaagccgta acgagcaata tcacttgcta tgactagtac
     7561 ttttcgttct ggatgatttt tgacatattc tttcgccatg tgcaaagctg cggtgccacc
     7621 gtagcaagcc tcttttactt caaaagaacg agcaaaagat tggattttca gtaaatggtg
     7681 gataattacc gcactcgctt ttgaatgatc aatcccggat tctgttccga cgatgaccat
     7741 gtcgattagc tcacggtctt tttctgttac gattttgctg gcagcatttg ctcctagtgt
     7801 aatgatgtct tcatttgcac gattgactgc catttggtct tgaccaatcc ctatatggta
     7861 ttttgcagga tcatccccac ggctttctgc caactctgtc atatctaaat ataaattagg
     7921 aataaaaaag gaaagacgat caatccctat tttcatttca acacctcttt acttatgata
     7981 aaaaacttac tttactaagg tatcataaaa agattaaaag aaatataaaa aagagaatat
     8041 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
     8101 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
     8161 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
     8221 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
     8281 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
     8341 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
     8401 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
     8461 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
     8521 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
     8581 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
     8641 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
     8701 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
     8761 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
     8821 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
     8881 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
     8941 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
     9001 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     9061 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     9121 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     9181 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     9241 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     9301 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     9361 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     9421 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     9481 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     9541 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     9601 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     9661 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     9721 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     9781 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     9841 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     9901 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     9961 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
    10021 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
    10081 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
    10141 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
    10201 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
    10261 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
    10321 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
    10381 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
    10441 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
    10501 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
    10561 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
    10621 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
    10681 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
    10741 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
    10801 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
    10861 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
    10921 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
    10981 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
    11041 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
    11101 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
    11161 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
    11221 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
    11281 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
    11341 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
    11401 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
    11461 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
    11521 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
    11581 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
    11641 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
    11701 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaa
//
LOCUS       DAZKCY010000076        11740 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000076 DAZKCY010000000
VERSION     DAZKCY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.077"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8XC_001912"
     CDS             6..305
                     /locus_tag="ACG8XC_001912"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9191602.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8XC_001913"
     CDS             378..1739
                     /locus_tag="ACG8XC_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9191603.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8XC_001914"
     CDS             1761..2021
                     /locus_tag="ACG8XC_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191604.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8XC_001915"
     CDS             2035..2787
                     /locus_tag="ACG8XC_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFP9191605.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8XC_001916"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8XC_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9191606.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8XC_001917"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8XC_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFP9191607.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8XC_001918"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8XC_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9191608.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8XC_001919"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8XC_001919"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP9191609.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8XC_001920"
     CDS             7161..8558
                     /locus_tag="ACG8XC_001920"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9191610.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8XC_001921"
     CDS             8639..9079
                     /locus_tag="ACG8XC_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9191611.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8XC_001922"
     CDS             9227..9553
                     /locus_tag="ACG8XC_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9191612.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8XC_001923"
     CDS             9554..10303
                     /locus_tag="ACG8XC_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFP9191613.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8XC_001924"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8XC_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9191614.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8XC_001925"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8XC_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191615.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3721 a         2027 c         2377 g         3615 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaaatta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag atttttttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcgggtttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg
//
LOCUS       DAZKCY010000077        11468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000077 DAZKCY010000000
VERSION     DAZKCY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.078"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..649)
                     /locus_tag="ACG8XC_001926"
     CDS             complement(164..649)
                     /locus_tag="ACG8XC_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFP9191616.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    739..1084
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1129..1770
                     /locus_tag="ACG8XC_001927"
     CDS             1129..1770
                     /locus_tag="ACG8XC_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9191617.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1789..2424
                     /locus_tag="ACG8XC_001928"
     CDS             1789..2424
                     /locus_tag="ACG8XC_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9191618.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2442..3257
                     /locus_tag="ACG8XC_001929"
     CDS             2442..3257
                     /locus_tag="ACG8XC_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9191619.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3576..5012
                     /locus_tag="ACG8XC_001930"
     CDS             3576..5012
                     /locus_tag="ACG8XC_001930"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9191620.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5201..7648
                     /locus_tag="ACG8XC_001931"
     CDS             5201..7648
                     /locus_tag="ACG8XC_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9191621.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7878..8945
                     /gene="rlmN"
                     /locus_tag="ACG8XC_001932"
     CDS             7878..8945
                     /gene="rlmN"
                     /locus_tag="ACG8XC_001932"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFP9191622.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8935..9141
                     /locus_tag="ACG8XC_001933"
     CDS             8935..9141
                     /locus_tag="ACG8XC_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191623.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9138..10241)
                     /locus_tag="ACG8XC_001934"
     CDS             complement(9138..10241)
                     /locus_tag="ACG8XC_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9191624.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10794..11342
                     /gene="aac(6')"
                     /locus_tag="ACG8XC_001935"
     CDS             10794..11342
                     /gene="aac(6')"
                     /locus_tag="ACG8XC_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP9191625.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3807 a         1941 c         2478 g         3242 t
ORIGIN      
        1 caataaatat ggatttggct aactatggaa gaatactcgt attagttttc agcgttcata
       61 ctagttgtta cattgtttga ttggccaaaa gaatttctga tatattttgt gctaaataaa
      121 gaaaaagacg cttgaattgg gaaataagaa aactactcta tcattacaaa ttttcataaa
      181 ctggcagatg ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc ataatcaacg
      241 tagtggtatt gatatttgga tgaaagccta cggtatcttg acgatcaata gcagcatcga
      301 tcacgacttg gatttttttc tctttgtcgt gcatcaatgc taatggagtc actgtacctg
      361 ctggaacgcc taggaggtca ggaagctgtt cttcagaaac aaaagataag cgcttttctt
      421 ttaattgttc agctagttgt tttaaatcgg cgtgcttttc atcaggcaag atgacaagat
      481 agatttgttt tccttttctt gatttcaata atagattttt tacttgcgga ccaggtaggg
      541 aaaaagggac atttttgact gacgtaatcg caggatgatc gactttttga aaagagatac
      601 ccagtttttt tagtagttca taagcttctt gttcggttgc acgactcatc gcgcatcacc
      661 ctcctttttt tattagtgta tcataaaaaa gagttgacaa atgaaaaata atcatgtaaa
      721 ttacacgtaa agttaaaaca aatgacatcg aaaagaagag tagccgttag gtgtttttag
      781 agagtctctg gttggtggaa agaggcagca gttaaccgtg aaacacatct tggagtcgat
      841 tttctgaagc gtatagtagg aaaatccggg gagcccgtta cagctgcggt cgttgctaga
      901 tgaccagata aggctggctt ttccgtgagg agtcggtaaa aaaaggtgga accacgtgat
      961 taacgtcctt tgatcttcgg atcaaaggac tttttgtttt catctgaacg gcaacgactg
     1021 tgtgaggttt tttctgcaag gaaaaaataa acagaggtgg aaccacgtat aatacgtcct
     1081 cttggcattt gccaagagga tttttttatt ttaaaggagg aaaagaagat ggagtatttg
     1141 attgaagtta tgccgcaatt attagtagga gcactcacaa cattgaaatt atttttattt
     1201 accttgattg gctcattacc attagggatt gttttagcat taggattgtc tttgcatttt
     1261 aagccgctac gctacttgct tcaaacatat gtatgggtta tgcggggaac accgctgttg
     1321 ttgcaactga tttttatttt ttatgggttg ccgacgattg ggatcgtttt tgatcgtttc
     1381 gaagcagcac tcattgcctt tatcttaaac tatgcagctt attttgcaga gatctttcgt
     1441 ggagggatac aatcgattcc tcaaggtcaa tatgaagcag cgcaagtttt gcaattaagt
     1501 ccctttcaaa cggtgacgaa aatcgtcatc ccacaagtga caaagattgt tttaccttct
     1561 gtagggaatg aagttatcaa cttggtcaaa gattcttcct tgatctatat attaggttta
     1621 ggtgacgtaa tgcgtgctgg gaaaatcgcg atggaacgcg acgcgacttt actgcctttg
     1681 ctaggcgtgg caatcatcta tcttacattg actggtattc taacaatcgt aatgaaacaa
     1741 gttgaaggaa aattaaatta ttaccgctaa ctgataggag gaagaaatat gattgaattg
     1801 aagaaagtga acaaagcatt tggtgaaaaa aaaataatca aagatttgaa tctaatcatt
     1861 ccagatggga aaacattagc tatcgtaggt ccttccggtg gcggtaaaac cacgctttta
     1921 cgtatcttgg ctggcttaga aagttctgac agcggagaat tttatttaga cggtgtaccg
     1981 ttcaacccat tcgagcttaa aatgaaagaa caagttgtag gagtcgtatt ccaagacttc
     2041 cagctatttc cacatctgaa tatttttgaa aacattacct tagcaccaaa aatggtattg
     2101 aaacagacaa aagaagaata caccaaaaaa gcagaagaat tagcggcttc attagggatt
     2161 agtgatttat tgaatatcta tccttatcag ctttctggcg gacaaaaaca gcgggtagcg
     2221 attgccagag cattggctat gaatccacgg gtattggctt atgatgaacc aacaagtgct
     2281 ttagatcctg aattacgcca gcaagtcgag cgtttgatcc tatcattgaa agaagatggg
     2341 gtaacccaaa ttattgtcac ccatgattta gtatttgcag aaaatgtcgg tgatcaatta
     2401 ctaaaagttt ccccaattca ttaagaaagg agaagataga gatgaaaaaa accagttttc
     2461 tattcgcttt gatcgcaggt cttcttcttt ttgcaggctg ttcgaacaaa aaaacttcag
     2521 cagataattg ggcgactatc aacgaagaga aaaaaatcgt tataggttta gacgatacat
     2581 ttgttcctat gggatttaga gataaagacg ggaagctcac aggatttgat attgatttag
     2641 ccaaagcagt ctttgaacaa tatgggatta cggtagactt tcagcccatt gactggtcaa
     2701 tgaaggaatt tgaactgaac aatggaacga tcgatttgat atggaacggc tattccaaaa
     2761 cgccggcaag agaaaagaaa gtgcaattta caaaacctta tatggaaaac gatcaagtgc
     2821 tgatcacacc taaagaatca ggaatcacaa actttggaca aatgaataat aagagattag
     2881 gtgcgcaaaa tgggtcatct ggttatgatg tatttacgaa acaaccagaa gtattaaaag
     2941 agatagtcaa aaaccaagat gctgttttgt atgatagttt taatgaagca ttgatcgatc
     3001 tgaaaagcag acgggtcgat ggtctgctga tggataaagt atacgcagac tattatttga
     3061 aacaaagaaa cgaactagaa gacttcaaca taacaaaagg ttcttatgag agtgaggatt
     3121 ttgcagttgg tgcacgcaag tcagatagta cattagtgaa aaaaatcaat gaagccctag
     3181 atcaactaga gaaaacaggt gaattccaaa aaatatccga taaatggttt ggcgaagatg
     3241 ttacgccgag aaattaacaa ataggggact gtgacaaaag acgtgtcaca gtcccttttt
     3301 ccgaataaac agtgttctcc tgccaactcc ttgtgactcg gagctgaatg gcaggctcac
     3361 aacctcttgg taaacggtgt tcaaaagcca gtttttcggt attaagccaa atttatgcaa
     3421 aaatgtagaa agccattttc acaaaatttt tcttactact cgaaactaag cgactttttc
     3481 acaacctcct ttacataatt ttattttgtt gtggaagggg tttcgtgtta ttctaaaaga
     3541 ggaacgattg aaggttatag gaaaggaaga gatgaatgga acacaaagaa cttacctcat
     3601 ttccaaaaga ttttctgtgg ggctctgctt ctgcagcgta tcaagtagaa ggagcttggc
     3661 aagaagatgg gaaaggagaa tcagtctggg atcgatttgt acggatacct ggaaagacat
     3721 ttaaaggaac gaacggtgat ttggcagtag accactatca tcgttataaa gaagatattg
     3781 ctttaatgaa agaacaaggt ctgaaagctt accgcttctc tgtagcttgg acacggattt
     3841 ttcctaatgg acgaggagaa gtcaatcaag ctgggctcgc tttttatgag agattgatcg
     3901 acgaattgat tgaaaacgat attgaaccta tgttgacttt atatcattgg gatcttccac
     3961 aagcattgca agatgaatat aatggttggg aatcacgtca gatcattgaa gatttcactc
     4021 attatgcaga aacattattt gaggcattcc gcgggaaagt ccattactgg attagtttga
     4081 atgaacaaaa tatcttcact tcattaggtt atttgttagc agcacatccg ccaggtgtga
     4141 cagacccgaa acgaatgtat gaagttaacc atattgctaa tttagcaaat gcatctgtca
     4201 tcaataaatt tcacgagtta gaaatgcctg gaaaaatcgg accaagcttt gcgtatacgc
     4261 caaattatcc aattgacagt aatcctgaaa atgttctggc tgctgaaaat gcagaagacc
     4321 tgatggccaa ttattggatg gatgtctata tgtggggaaa atatccgatt gctgccatga
     4381 ggttcttaga agaaaaagga tgggcaccaa cgattgaagc tggcgatgca gaattgctag
     4441 aatctgctaa accagatttt cttgggatca attactatca aacggcgacg aatgcattca
     4501 atccattaaa cggagttggt gcaggaaaaa tgaatacgac aggaaaaaaa ggttcatcag
     4561 aagaaactgg agttcctgga atgtataaaa aagttgagaa tccatttgtc gaacgaacaa
     4621 attgggactg ggagattgac ccagaaggat taaggatcgg actgcgtcgt atcacaagcc
     4681 gttaccgcat gccggtactg attacggaaa atggcttagg agagtatgac aagttgacag
     4741 aagataaaca aatccatgac gactatcgaa tcaattactt acaaagccac gtcaaagcca
     4801 tcaaagaagc aatttcggat ggagcagaag tcctaggcta ttgcacctgg tcttatacag
     4861 atttgcttag ctggctgaac ggttatcaaa agcgatatgg atttgtatat gttgatcaag
     4921 acgaaacaca agaaggatct ttggaacgat ataagaaaga cagttattac tggtaccaaa
     4981 aactgattga agaaaatgga gagaaccttt agttcacata cttaaaacaa gaaggtacgg
     5041 ctggatatgg ccgtgccttt tcttgataaa atcatttaga aattaagaaa aactgtaaaa
     5101 taagctgaaa aatgaaagaa agcgtcagtt ctttcaaaca aaactttagt tcccttactt
     5161 ttttttgtat aatgaaactt gatgaaatga gggataataa atggcacaaa agaaacggcc
     5221 tactaaaaaa aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc aggaacaaat
     5281 gctgacgatt ggtgttgggt ttgccttgat tttatttgct gtcttcggct ttttaaaatt
     5341 aggtttttta ggaatattga ttgcaaacgg ctttcggatc attgcaggaa atacttacca
     5401 aatcctctgt cttttattag ccgtattagg attttggatt gtcattaaga atacagagtt
     5461 ttcaattggt aaaaaccgac gctggtttgg cggcatactc ttttatttcg gtatcttatt
     5521 attgttacat gcccatttat ttggcaaact ccatactggg gaaccaaata tcatggggac
     5581 gacatgggat ttgttagcca gtgatataaa acaaagtcaa gtagacaata acgttggtgg
     5641 tgggatgatt ggagcaatcc tttatcattt tacttacttc ttgattgcac agcctggaag
     5701 ttatttagca gcagtgctat tactggcagg aggagcattc ctgttcagta atctggaagg
     5761 ctatcagctg ttaaatggta tccaatcaat tggagaacga gtacaggaat tattggaagg
     5821 tgatcctgcc aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc
     5881 agaagcgaaa gaagcaaggc gcttagctgc aaaagaagct gcagaaaaag aagctgcaga
     5941 atatgagaag aaaaaagcgg tgagccagca aagggaaaat actgctgatg aatggcagga
     6001 accagaaaat acagagccgg agcagctaag ttttgttcca atcgacagtt ttcaagaaaa
     6061 tatccatcct gcgaatctag agaaaccagt accagataca ccaaaacaga ccaatacagc
     6121 agaaggtttt tcagatgagt tgccagagga tgacggtacc tcattagaat ttgagattga
     6181 agcagaacag gaaaatcaag actatgaatt accgacagtt gatttgcttg attctatacc
     6241 aactgtggat caaagtgatg agtataaaaa aattgaaaaa aatatcggcg tattagaaca
     6301 aactttccaa agtttcggtg tcgatgccaa ggtcgtcaaa gccagcttag gaccatccgt
     6361 cacaaaattc gaagtccagc cggctgtagg ggtaaaagta agtaaaattg tgaacttaac
     6421 agatgatatc gcgcttgctt tagcggcgaa agatgtccgg atggaagcac cgattccagg
     6481 taaatcactg attggtattg aagtaccaaa tggaaagatc agtatggttt cctttagaga
     6541 aatcatcgaa gcacaaccaa accacccaga taaattacta gaagtacctt taggacgtga
     6601 tgtttctgga cgggtacaaa cggctgatct ttcgaagatg cctcacttac tcgtggctgg
     6661 ttcgactggt agtgggaagt cagtagcaat taatgggatt atcacaagta tattaatgcg
     6721 tgctaagccc catgaagtaa aactgatgat gatcgatccg aaaatggttg aattgaatat
     6781 gtacaacggc atacctcatc tgttgacgcc ggtcgtgacg aaccctagaa aagcagcgca
     6841 agccttacaa aaagtagtac aagaaatgga agaacgatat gaaaaattcg cggcaaccgg
     6901 agtaagaaat atttctggtt acaatgaatt tgttcaacag aaaaacttgg aaaacggaac
     6961 gaaacatcca accttaccat tcatcgtagt gatcgtagat gaattagctg atttgatgat
     7021 ggtagccagc aatgaagttg aagatgcgat tattcgttta gcgcaaatgg ctcgggcagc
     7081 ggggattcat atgatcctag ctactcaacg tccaagtgtg gatgttatca caggaatcat
     7141 caaagcaaat gtaccttcca gaatggcatt tgcggtatca agcggtacag attctcggac
     7201 gatcattgat tctaatgggg cagaaaagct cttagggcga ggagatatgc tattcttgcc
     7261 aatgggtgag aataaaccga tccgtgtgca aggagcattc atttctgacc acgaagtaga
     7321 acgggtcgtt caatttgtta cagatcagca agaagctcat tatgaagaga aaatgatgcc
     7381 gacagatgaa gttgaaacga caggtgctcc agaacagcca caagatgagc tgtttgaaga
     7441 agcaaaagca cttgtcgttg aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt
     7501 tcggatcggt tacaaccgag cagcacgttt agtagatgaa ctggaagctc atggagttgt
     7561 tggcccgtca gaaggaagca agcctcggaa agtattcatt gagcaggaag aaacatcaga
     7621 aacgttgccg gacgataatg acatgtaaaa cttgtgtgat gaagggttga aaaacgaata
     7681 accaataaaa agacaaggca tgatgatacc aaaagaaaaa atggtttttt tccatctttt
     7741 ctaatacggt atgatgtctt gtctttttag tttttgtgag attaaagttc tattcgattt
     7801 atttgtgaag tattgcatta aggaaatgaa tcatgtatag tatgtagcga aactttgaga
     7861 aaaagaggga aaatactttg gaaaaacagt ccatttatgg cttgacgaat gaagaattaa
     7921 tcaattggtt tatagaaaac ggcgagaaaa aattccgagc tgcccaagtt tgggaatggt
     7981 tgtatcaaaa acgtgtaagt aatttcacag aaatgacgaa tctatcgaaa caattgatcg
     8041 aaaaattatc ggagcatttt atcatcaatc ctctaaaaca gatggtcgtc caagaagctt
     8101 cggatggtac agtaaaatat ttgtttgaac tccctgataa aaacatgatt gagactgtac
     8161 tgatgagaca agaatatggc ttgtcagttt gtgtgacgac acaagtagga tgtaatatcg
     8221 gctgtacgtt ttgtgcaagc gggctgctca aaaagaaccg tgatctgact gctggagaaa
     8281 ttgttgcaca gatcatgatg gtccaacatt attttgatga gcgcaatctt ggcgaacgcg
     8341 tttcacatgt cgttgtaatg gggatcggtg agccatttga taattacgac aatgtaatgg
     8401 actttttgca tatcatcaat gatgccaaag gattggcaat tggtgctcgg cacattacag
     8461 tttccactag tggattagca cacaaaatca aggaattcgc taataacggc ttgcaagtca
     8521 atctggctat ttccttgcac gcgccgaata atgaggtgcg aacatcgatc atgcggatca
     8581 accgaagttt cccaattgag aaattgatgg aagctgtgga tgagtattta gagaaaacaa
     8641 accgtcgaat cacgtttgaa tatatcatgt tgaatcaagt aaatgataga ccagagcatg
     8701 cgcaacaatt agcagatcta ttaaaagata agaaaaaatt agcatatgtc aacttgattc
     8761 catataatcc agtcagtgaa catgatcaat attctcgcag tccaaaagct gatgtattga
     8821 gattttatga tgtattgaag aaaaacggcg taaactgtgt gatacgtaaa gaacatggta
     8881 cagatattga tgcagcttgc ggacaattgc gaagcaagca aatgaaaaaa acagttgcca
     8941 aataatcgta tcctactcgt tttttcaatt accataaaag gagcagtttt cttaattaag
     9001 gaactgttca acttaaataa gagaaaagat tctatagcga aagctataaa gctttttttg
     9061 acaagaaaaa ccctcatcaa gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg
     9121 ggggttttag caattattta actgattttc ttatatcctt gctggctttc ttgtgtagca
     9181 tgataagttt ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat ttcataaggc
     9241 acatgtttaa tatcatttgt tgccatggct ttatctaaga ttgcttctaa atcggtcgtg
     9301 acattgagat tcatgtcggt taacgatact ggaagttggt tctttttcat aaacacatag
     9361 attttatcaa acaaatcgaa ctgctgatcc atcatcaata aggtcagtac accataactg
     9421 acagatgctc cgtgaaggta tccctccatt ttttctagaa cagtcgtacc gtaatagaat
     9481 gagtgtgcca tagcagaatt atagttattg ttaactaaaa cagaaaccaa tccggtagta
     9541 acaatgatat ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc ggcactatct
     9601 ttcaatgctt gcgtaccata agtcaagaga cgatcaatgc acatttttcc gatttggatt
     9661 cctaatctat ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga ttcgacttct
     9721 ttggataaag catcgccaat acctgcccat aagtatttat caggtgcttc ggcaataatt
     9781 gctgtatcaa tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc gtccattacg
     9841 ccttcttcgt tatagatgac acataccgca gtgacgggcg aacagttcga accaatagtg
     9901 ggaaaggtga agactggttt agctagtttt tcaccaatga cttttacagt atcgcaagca
     9961 cggccaccac cgactgcaaa caggacgtct gctttttgga cactttcatg ctggatcaac
    10021 tgttcaatat ttccataagt tgaattaccc ccgtaccaga gactggcgac aacctctatt
    10081 tcctgtgtca aagctacttt tattttgttt tctgcctttg aaagtgcagt cttccctcca
    10141 atcagcacta cttttgtccc atattctttg acgattccat ttgctttttc atacgcatct
    10201 gttccaatgg tataatttgg taagaaaaca ctttgactca tattcatttc cttctttctt
    10261 cgttgaaacg ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt agatggagta
    10321 gaggtgaacc taagacagga taagtgatga ataaggtggc atgaacacct tgaaatcatc
    10381 aataagtatc ctaaaaacga acaagtacaa aaacattgaa aacgacaata atgaattttt
    10441 gttcctctta gtatttctac cgattccctt tgcttttttg taattatcta aaaattctaa
    10501 aaattaagat tattatatga aaacaggttt tatttgtcaa ttcataattg gtttttttta
    10561 aagcaaaaga taatctatat catgatgaac aaacagagtt ttttcctcca attgatggct
    10621 ttcctgatct ttcctatttt gaataaagtg tttctgtata atttttttat tttataagta
    10681 agataaataa tgaaagaata gagatactga ttggtagcta aagatttttg agcgagaaca
    10741 tttcgttttt tcaaaaggac tgttagaata agcaagaaca agggggaaga cgtatgataa
    10801 tcagtgaatt tgaccgtaat aatccagtat tgaaagatca gctttctgat ttactgagac
    10861 tgacttggcc ggaagaatat ggagacagct cggcagaaga agtagaagaa atgatgaatc
    10921 cagaacgaat cgcggtagca gcggtagacc aagatgagtt agtaggattt attggtgcaa
    10981 tccctcaata cggtatcaca ggttgggaat tgcatccatt agttgtagaa agctcccgac
    11041 gaaagaacca aataggtact cgattagtca attacttaga aaaagaagta gcttccagag
    11101 gaggaatcac gatttattta ggtacggatg atttagacca tggaacaacg ttaagtcaaa
    11161 cggacctgta tgagcataca tttgataaag tggcttccat ccagaacctt cgtgaacatc
    11221 cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca aatgcaaatg
    11281 gctgggacaa accggatatt tggatggcaa aaacgattat tcctcgacca aattctcaat
    11341 aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa ataagaagga atttacgaaa
    11401 attacttctt gaatttttgt gaaattccaa cttatttccg aagaagctac ttttggaaca
    11461 ccgtgtac
//
LOCUS       DAZKCY010000078        11437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000078 DAZKCY010000000
VERSION     DAZKCY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.079"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..1323)
                     /locus_tag="ACG8XC_001936"
     CDS             complement(28..1323)
                     /locus_tag="ACG8XC_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9191626.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
     gene            1651..2130
                     /locus_tag="ACG8XC_001937"
     CDS             1651..2130
                     /locus_tag="ACG8XC_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFP9191627.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            2163..4391
                     /locus_tag="ACG8XC_001938"
     CDS             2163..4391
                     /locus_tag="ACG8XC_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9191628.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            4520..6163
                     /locus_tag="ACG8XC_001939"
     CDS             4520..6163
                     /locus_tag="ACG8XC_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9191629.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(6236..6781)
                     /locus_tag="ACG8XC_001940"
     CDS             complement(6236..6781)
                     /locus_tag="ACG8XC_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFP9191630.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(6804..7289)
                     /locus_tag="ACG8XC_001941"
     CDS             complement(6804..7289)
                     /locus_tag="ACG8XC_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9191631.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(7279..8091)
                     /locus_tag="ACG8XC_001942"
     CDS             complement(7279..8091)
                     /locus_tag="ACG8XC_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFP9191632.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(8230..9762)
                     /locus_tag="ACG8XC_001943"
     CDS             complement(8230..9762)
                     /locus_tag="ACG8XC_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFP9191633.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(9871..10902)
                     /locus_tag="ACG8XC_001944"
     CDS             complement(9871..10902)
                     /locus_tag="ACG8XC_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9191634.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3764 a         2115 c         2201 g         3357 t
ORIGIN      
        1 aaagaagaaa acctctttct gttctgttta tttggttaaa aatgttttgc catgacgaat
       61 cataattcgt agtttgaaat tgtaaaagtt gcgaaagccg tacgcattgc gttttaatac
      121 tttgatgtgg ttgtttaagc attccaatgg tccgtttgaa tagctgtagt gaagcgcatt
      181 ttggatctgt ggcagaaact ttttaaacgt tcgtaaagtg gttgtatagg tttctggcag
      241 accgctaatg tctctagtta aacaatcctc aaatagtgac tgatctcttt tctttaccgc
      301 ataaagaatg tcttggtata actgatagcc ttggcgtaag ttgggacaag cagctaacag
      361 tcgatcggca atctcacttt cagtcaaata atctttaaaa ctggcacgcc aaacccgttt
      421 ttcatagttt aactttgctt cttctttttg taggagtttc caatagcgct tcaatttttt
      481 ccctaaccct cgttgagagt gggaatttcc tttaagtcgt accgtggttt ctttcactcg
      541 atgattcaag aaagttctgc caatatgttg gacgatgtgg aaacgatcga tgatcaactg
      601 agcattcggg aaaagctttt tcaccaacga aacataaggc gcatacatat caataacaat
      661 caattgaacg gcttcacgag tcgctaatga aaaccgagaa aaatagcgtt ctaaataagg
      721 caagcgtcga ttttcgacaa tatccaataa tgcatggctt tgtccgtcca tcatgataaa
      781 actcattgca ctggcaacac ttcgaaccga tttgaattca tcaaagcata agacctttgg
      841 caaagtctgt ttgaaaggat tcaatgggcg gtagaatcgc ttcatgacac gatagaccga
      901 agaagtggaa atatttttct gacgagcgat ttccgtcatg gaagtattct cttttaagcg
      961 agcaatgaca gaccaacgaa ctttttgact aatgaaacag tgtttttcag caacagaggt
     1021 atccgctacg aatgttcttt ggcaagactt acatttaaat cgttgtttct tcagctccag
     1081 ataagtgcga tactcagaca catcattcag taacaaccga acggttgtcg tcccccaacg
     1141 aatgatccta gaggggacga cacaaagaca atgggaacac tcttctggct tgtacagaag
     1201 agttcctttg taaatcaagc aaatttgccc tttgtatttt tctttagtta aacaattttc
     1261 ctcgaaatag atatttaggt ctagaatatt tagggtatct ttgataagct gagtatgaga
     1321 catcttgaat cctcctatga attggtagcc aaattaattc taaaggattt cagggtgtct
     1381 tttctatatt tttttataca aaaaagatgt tggtgaagtt caccaacatc aaaaagtata
     1441 gagccaaaat attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa
     1501 gaacgagatg gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc
     1561 aatcctctat aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta
     1621 ctaaaaataa aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa
     1681 ccagtaatcg ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat
     1741 gcaaaagggc tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca
     1801 ggcaaggtat ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc
     1861 acacgtattt tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct
     1921 tatcgttatg aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa
     1981 gggaaccgaa tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt
     2041 ttgtattacg atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta
     2101 aaacgtcttt tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa
     2161 gcatggagaa tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga
     2221 tctggaagaa accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa
     2281 tcaaagggaa ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg
     2341 ccgggtcggg gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata
     2401 caaaagtgac ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg
     2461 tagtagattc taataaggaa caagaccaag aattactaga agctatttac caaacagata
     2521 cacttgaagc tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta
     2581 aagaaaaatt agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt
     2641 acagctatta cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc
     2701 aagagccgac tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac
     2761 caaatgggat gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg
     2821 tgatcactac aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc
     2881 ggatgcaaca tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga
     2941 tggcgaaaac tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt
     3001 tgttggatga aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt
     3061 tttattgggc aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag
     3121 ttttgtataa attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg
     3181 acagtggatt gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc
     3241 aatctgagca tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca
     3301 ctgaaaatgc tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac
     3361 caaatgaggg agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa
     3421 tgcaaaaaga acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag
     3481 atgattctca agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc
     3541 aactgaataa agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca
     3601 tcgttcaccg accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag
     3661 taatgccaat agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa
     3721 cagagacgta ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat
     3781 atggtgattt gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg
     3841 tagctgtacg gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa
     3901 aaaaagcagc gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt
     3961 acggagatac agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat
     4021 ttgaagacgg atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca
     4081 atcgtgatgg tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa
     4141 ccaaactgtt gcttaatatt agtgacggtc agccaaaagc attgccggat tatacaggca
     4201 aaaaagcaaa agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat
     4261 ttgtctccgc agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc
     4321 gttttgtaga tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc
     4381 gctatctatg ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt
     4441 ttctagttac cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc
     4501 gttaaaggag tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta
     4561 tcaaatctat cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg
     4621 gatccgtgaa aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc
     4681 gatctatcaa tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga
     4741 agagagattc ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg
     4801 gatcaaagtg attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga
     4861 agaatcgaaa aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt
     4921 agatggcaaa gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc
     4981 tcatgaaact ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg
     5041 ggaaagcgag aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg
     5101 gatagatgga tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa
     5161 agccacagag aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac
     5221 cgatttgaaa catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt
     5281 cactgcagaa gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga
     5341 atttgatcat atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt
     5401 gaaacatgca ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata
     5461 tatggaaaac catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt
     5521 ttgggctatg tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt
     5581 tatttatcaa ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt
     5641 tgatgcagta gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga
     5701 agaagcattg gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg
     5761 gacttcggaa caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa
     5821 taccaaaacg atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta
     5881 taagaacatg atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga
     5941 atatcttcat gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata
     6001 tcttattatg gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga
     6061 tcagtcctgg acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat
     6121 actagcacct tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt
     6181 tcattagttg gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat
     6241 aattcgctct aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt
     6301 gtccaagggt tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga
     6361 ttgatgtgtc accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat
     6421 cattgaactg gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa
     6481 cagagacaat ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt
     6541 tcaaatgttc acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta
     6601 acgtacggat gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa
     6661 cgacttcgct tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag
     6721 ccaatatgtc attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca
     6781 ttttctaaaa ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt
     6841 gcagttatag gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata
     6901 gaagccactc cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc
     6961 ataactaagc ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa
     7021 gaaccagcaa catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga
     7081 tatcctgaaa tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt
     7141 ggaccataaa gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc
     7201 gtagccggta cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata
     7261 gcatagatcg ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga
     7321 ccagacactt ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa
     7381 ttttttactt tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat
     7441 tgcagaagaa aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga
     7501 aaatacctca taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct
     7561 tgtgccgcct ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg
     7621 gatcatttct gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa
     7681 attaggcgta atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa
     7741 ttgattgatc acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat
     7801 attttgtttt gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa
     7861 cccaattttg ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag
     7921 caattccaaa gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga
     7981 agtcaaggcg cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat
     8041 tcctccacca gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc
     8101 ctccatgaat aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt
     8161 ttatctcatc caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc
     8221 cttaaatagt taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt
     8281 cattgtagct gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg
     8341 aagttcatgg acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc
     8401 aataatcggt tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc
     8461 taacgaatct aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc
     8521 cacttgccga tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct
     8581 atcccaccaa tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa
     8641 agcccgtcct ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc
     8701 aactgaatct gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc
     8761 atcacttgtt ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact
     8821 ttggccatat acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt
     8881 cattgtctgg ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc
     8941 ttctggatgc tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag
     9001 tgcaattggc cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa
     9061 aggcatcata ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg
     9121 cactggagag atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt
     9181 ttgccatttc gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc
     9241 agttgtacca gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga
     9301 aacagacaaa gcaggttgtt ccattaaata aagaatagga atctgttttg attttgttcc
     9361 atttgaaatt tctctcactc gcttttttgt ctcatcatca aataaaatga aagggtcttc
     9421 taaacgatcg ataaatactt ccattgttgt tgttggtaga tgataagaag tcatgacagg
     9481 cactgcacca aggtaagaca cagaaacagc tagaaggtat gtgtcaaaag cggaactctt
     9541 ataaataata actttgtctc cggatttcac tccaagatga gccaattgat aagcccgttt
     9601 caacacactt tcgcagcatt ttttgtaagt tgtttcaagt cctaattctg ggaaagctga
     9661 aagtggctca tcaaagataa tcggggtctc ggaaaagtta gctgcagctt cttgaaaatt
     9721 cgtaaataag ttcaaagggg aatacgttat ggtctccatc attcacactc cattatcttt
     9781 tgttaagtaa tatacaaatt caagataatc gtaacataat cctttatata ataacatagt
     9841 ttattgtgaa aaaaagatca tacatttgtt ttatatggaa gaaacccgaa cgaatgccgc
     9901 taattcgtga taagaaaaat caaccatctt ttcccagata tcttgaaaag aatgggatga
     9961 gaggtatcga ttaccacata aataatttac cgcgtcagca tcatctaaat atagttctgc
    10021 ataataacga tatgtttttc tgcatagttc tggcgatacg tttttatata ctgtaatttt
    10081 gctcaaaatt tggcgaatag tccgaggaga aatttgctga tcatgtgagg aacgaaaaac
    10141 aaaagaattt tcgtccgtat tttgtgctgt taacttaaaa tagttctcca gaaatggcat
    10201 cgcttctttt ggtattggta ctttattcag tctgttttct tttctattca gattaagaat
    10261 ctgcttggtc aaagatacat ctttacgttt aagttctact acttcatgga tctttaatcc
    10321 gcaatacaga agaatggcag taattgcaag gttcctcggt ctattctttt cttcaaagct
    10381 agcttctctg cttgtttgaa agccttcaat cgggataaac aaatgctcaa aatctttgaa
    10441 aagttctgca tcgatatatc gcggagctgc tttggttgtt tccggtaaac ttgtacccaa
    10501 aagagaaatc tcttcttcaa tcagtccttt ttgatacaaa aatcgtaaaa aatgatttaa
    10561 tgaatattgc ttccttgaaa tcccgtaata gctattttca aacaattgct ccgtttcttg
    10621 gttcgctgta gtttggaacg aacgagtatg tcgatagatt ttagctaaga aattctgata
    10681 atcttgtttt tctaaatgga taatcagctg atcatccact tcttcagaaa atacttttct
    10741 caaataatca gtaaacagat gaaaatcttt tgcgtattca tagcgactgc ctaatgaaag
    10801 ggaagaacga ttttcttcta aaaactgttg aaagacggaa gggagcaagt gtgtacgttg
    10861 ctttaacttt tcttgcagtt gttttctagt tttgctgtcc attacaaatt ccctccaata
    10921 gtgttcattt tctatatttt accgcatttc atcaaatgaa gatagacagt ttgtcttgat
    10981 cttaggaagc aaatttttaa ctaagtacta gctaaaagat atgaagtaag aagatacatt
    11041 tttagttatt ttattagtgc agtcattctt ttgatctttt aattgattta tcctcttttt
    11101 agctatgtgg ttacttcatg agcaatgaaa tggatttggt tcgttctaat ggcaggaggc
    11161 tctttctttt atttccgaaa aattagaaaa cattttttca gaatcgattg gctctccatt
    11221 tattcaataa tggtttgtct aacgatattg ttgggataat tcttaaagaa gtattagaaa
    11281 tagttggtac ttcattactt tacatgaaga tttattctat ggtaggagca attatattga
    11341 gaattaatcc ttttttatat aagttgtttg cttattgatg gattggataa attaaaccat
    11401 cacaacgagt ggaaattata tgaaattttt cataaaa
//
LOCUS       DAZKCY010000079        11417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000079 DAZKCY010000000
VERSION     DAZKCY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.080"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            224..430
                     /locus_tag="ACG8XC_001945"
     CDS             224..430
                     /locus_tag="ACG8XC_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFP9191635.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(632..1633)
                     /locus_tag="ACG8XC_001946"
     CDS             complement(632..1633)
                     /locus_tag="ACG8XC_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9191636.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1638..3551)
                     /locus_tag="ACG8XC_001947"
     CDS             complement(1638..3551)
                     /locus_tag="ACG8XC_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9191637.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3719..4225)
                     /locus_tag="ACG8XC_001948"
     CDS             complement(3719..4225)
                     /locus_tag="ACG8XC_001948"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP9191638.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4385..4825
                     /locus_tag="ACG8XC_001949"
     CDS             4385..4825
                     /locus_tag="ACG8XC_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9191639.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4851..5879)
                     /locus_tag="ACG8XC_001950"
     CDS             complement(4851..5879)
                     /locus_tag="ACG8XC_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191640.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(5934..6008)
                     /locus_tag="ACG8XC_001951"
                     /pseudo
     CDS             complement(5934..6008)
                     /locus_tag="ACG8XC_001951"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6011..6367)
                     /locus_tag="ACG8XC_001952"
     CDS             complement(6011..6367)
                     /locus_tag="ACG8XC_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191641.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6665..7639)
                     /gene="sgrA"
                     /locus_tag="ACG8XC_001953"
     CDS             complement(6665..7639)
                     /gene="sgrA"
                     /locus_tag="ACG8XC_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFP9191642.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7834..8673)
                     /locus_tag="ACG8XC_001954"
     CDS             complement(7834..8673)
                     /locus_tag="ACG8XC_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP9191643.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8858..9745
                     /locus_tag="ACG8XC_001955"
     CDS             8858..9745
                     /locus_tag="ACG8XC_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9191644.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10339..11034)
                     /locus_tag="ACG8XC_001956"
     CDS             complement(10339..11034)
                     /locus_tag="ACG8XC_001956"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9191645.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11018..11416)
                     /locus_tag="ACG8XC_001957"
     CDS             complement(11018..11416)
                     /locus_tag="ACG8XC_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9191646.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3403 a         2289 c         1705 g         4020 t
ORIGIN      
        1 tcctaaatct tttctattat attcccatat tttctattat tggtctagac gtatgatggt
       61 aaactttgta cctatctgat tgctgaatca ttaattgctt atcattagcc atctttgttt
      121 caagttcgct agatgatcat tttattcaca tcctaacaaa cttgtcacat ttatagattc
      181 ttaatctatt tatcatacaa ttataaaaaa ggaggtgctg cttatgaaag ctatccatat
      241 ttctttgctt tttttcgcta gtgcattgat tttatttgct attcatcttt gttcccagcc
      301 ttatatcaat aacaacggaa tattagtgga accagcattc ttttgcttac cactcggctt
      361 tttgtccctt cttgcctccg caggcttcgg atttgttgct ttttttaaac gatcaaaaca
      421 acaaatctga cttataaaat ttacgtaaaa gagatcgtga caataatcgt gtgatattcc
      481 caaatttcga ataaaaggtg ccccttgcca tctctaactc ctcagtcaca agttacaata
      541 ttcataaacc atgaaaagct tgtttattga aattgctcct tgtgacttgg agctaagtga
      601 cagattacaa cctcttttgc atcataagac tttactcctt gaaaaacatt actaactttt
      661 gatatgtttc ttgcgcttgt ttattgctga aatcaaactg atattcatgg ttcaaaacag
      721 catttttctc cgcataatct acttttgtga ctttcacccc cgctttctca aatacagaag
      781 ttacttcttc catttgtttc gtaaacgtat ttgtatttcc atccgttaag aatgtgtttt
      841 gcgtccaacg atccgcatgt ctgaccaagg ctaaactttt gaccttctct gattgttcaa
      901 agtccttatt atcaaagtaa gcccatccac atttttcata aagaaagcta gagattttat
      961 cacttacttg agtaagtgca gaaggatcca acagaccaga caaagaaata aacttcgtaa
     1021 tctgcttgtt ctccaaagtc ggatcaagat gatcagcttt tttaatttct tcattttgaa
     1081 gtgctaacaa atattctcca ctaatttgtg cacctgccga atttccacca atcgaaatat
     1141 tcgaccaatc tgcttgatct tttatctctt ttttagctgc ttgaatggct tcgttcaatt
     1201 ggataactgg tgtaggatgt tttgcttccg gtgatcgttc ataatctata tttacgatga
     1261 tttgctgcgt gtctgaacat agatattcca aataatcacg acaatcattt ttatcccctc
     1321 ctacatatgc gcctccatgc atccagaaaa gaatcggtaa ttttttcttt tccactttcg
     1381 gtgtgtagat atccatatta ttatttttgt actttgaagg ataggatatt tcttttttta
     1441 tatcgatttg ttcgattttt tgctggaaat catctggtcg ggcatatact tgttcttttt
     1501 cacttgtgtc aaattgtttt ctaacaagca agctaatcgg ctttggtgtg atttgattaa
     1561 tcaaaaatac agtgactaaa ataactacga tgataactgg tactgcaatc actattcgct
     1621 tgaaccattt cattcattta ccctctcttt cttcgccaaa tccatgtgat aatcgtgcta
     1681 acagataaaa atccacctga ataaatgaga agcgaatgtt tcttttctcc agttttcggc
     1741 aacattcttt tttttgcatc tgtctttttt tgtactttca ctgtcatctg aatcggttgc
     1801 gtatcatcac ttgattgatc ctctcgaggt tcattctttc ccttattgtc tgcattctct
     1861 gtcggatctt gcttttttgg tggagctact gggatttttt tgtaatagaa aataatagtt
     1921 ggtatcgtct cattaaaaat cccttcagtc ggtccttcaa catttgctaa ttcgtatcct
     1981 tcgaatgtct tttgctctgt catataattt tctccataag ctccgtaaag ctggatttcg
     2041 tcctggtctt tgagtagact tttattgttt tcatccacat actttacgtt tatctgatca
     2101 tggaacacta ctttcaaaaa gtccgttaca tgtttcgtgt aggcagcatg gtcaacttcc
     2161 tgtgcctttc catgccctgc tccttctact aataaaagat ctttataccc tcctgataac
     2221 tcatataatt tttgcgcgtt agacacaggt actacatcat ctgctgttcc atggataatc
     2281 aatttaggca atgaatctga cattccaatc gtaggtagag cagcttttgt atccaaatgc
     2341 atctcctgat caaaatagta ttgtttcaaa aggcccataa ataaatcttc atgctcttga
     2401 tccccaatga tgtctaactg attttttatg gcatccggta gtgcgggggc tactgtctgg
     2461 ctgtacgtaa aacgtagttg ttcatcaatt gatgcaaatc cgcaattttc aataattcct
     2521 ttgacattga caggaatatc tttcgctaat acagacatca tcgtagctgc tcccatcgaa
     2581 ccaccataaa gtaaaatctt ttgagatggc cgctctcgaa cttcttgatt tatccagtaa
     2641 aggacgtcat ctgactcata ttgtccatag gtcacataat cgcctccgct attaccagtc
     2701 gctctggaat caacaaataa taaattatac ccggccttat aaaaatcatt gtattcttct
     2761 gtaacgatcc cgttattcca attccctcga aatccgccat ggatcatgac tgttttatct
     2821 gaaccttgat cgacataata tgcatataca tccatcattt tgccatcttg tttgtttagt
     2881 acagtttgat gaagttgttt tgtatttgga tagaagcctg tgttttctgt aggcgttgtc
     2941 ccatcatctg tacctccaag catcgacatc aacacactgt tttttgcaaa agcgatatcg
     3001 tacaatagat aagtagcaat cagatgtttg ataatctctt tatctacacc ataatcatta
     3061 acagcaagct cagtgactaa taactctatt aattttgact tgatgtcttc tgttggtcca
     3121 ttaagtattt ctgtcatctg tttaccgaag taattcaaat attctttacc attgaaattt
     3181 tcacctttac cgatatcatc attttctgtc ccattctctt tgtcacttac tttagcagaa
     3241 gaggattcgt cgtttgacat ttttgtagct gttgcttctt ctgtttgttg gttatctgtg
     3301 gtagcggtcg tttcttgatc tgtgctagta acagatgtct caaccgttgt ttcagtattt
     3361 gtttctgttt gtgaagtact cgatttcgtt tcatttccag catcagtagt ttctgtggtt
     3421 tctacctccc gagattccga ttcggaagaa atgctctctc catatactgt ccattgcggg
     3481 acgcttgtac ttacagcaaa taagatgacc aacgctccct ttaatacttg ttttgttctc
     3541 aagctattca ctccttttta tgtttctagg agaattatat gtaaacgcaa acatttttta
     3601 tacaaaaaat aaatggttta tcccataaaa atagaacagg attattttct cattttacgt
     3661 aataaagcag aatggataaa ccagtatcca ttctgctttt tttaaacgaa gaaacaactt
     3721 attgctccaa aaatgtcaga aatcgtttat cccaaatttt ctcgtaaaaa gcgattgttt
     3781 cctttgcttt cataaaagga ttatcataag ttgtcgttgc tcctctacac atcataagct
     3841 ggtatcctaa tccatgagca aacttcacag tcgtatctat acaatactct gcttgtgccc
     3901 cacataattc gatcgaatga cagtccaatt cagttaataa aacactcaaa ttagtcttat
     3961 aaaatgaatt cgcatgggtt tttctcacaa aataactgtg ctcaggaata gataattccg
     4021 acaatagctc ccatggtttt gtttgtggca ctaattgagc atcttcgtgt tgaacgaaaa
     4081 taactggttt cccaatcgtt tcgtatgttt tgattctttt attcactaat gctgtcactg
     4141 tttctaattc gtgtatcggc gaagaatcaa aacatacccc tttttgtaaa tcgatcacta
     4201 ctattgcgtc ggctgtgttt gacatacttg tgccttcttt cttttttctt gatttaatag
     4261 tatttcttat ctagctagtt ccattatacg tgacaacatt ttcttatttt acacctctaa
     4321 atggcacttt tcgactattt cttcatgtta ttatttcata aatacttagc tattggagga
     4381 atgtatgatg attaaattgt atacaggttc tggctgctcg tcttcaagac gcgcaaaacg
     4441 ttggcttatc gtccatgata taccgtttat cgaacagaac ttaattcgtc aaccactaac
     4501 caaaaatgaa tttatccact tactaaaatt gacaaacaac ggcgtgaccg aaatgctctc
     4561 tactcattct attagttata aaaatcttgt tcacagaata gatgaactca gtttgagtga
     4621 actatatatt gagattcgta catccccttc tttattgaaa aaaccgatta tctacgacca
     4681 atctgcaaaa cttttgactg gatttagcga agataaaatc aggatgttcc tttctaaaac
     4741 taaaagaaag caagagctca acgaactctt gctttctggt catcttgtcg attttaaaga
     4801 aaataatctt tttgcaatgt cttaattttg gcataaacaa tctgcttttc ttaattaacg
     4861 aaatccaatt tttccaatat ggatctattt ttctcattcc tgatttttat gatttcagct
     4921 ttcagacgcg cccaatcatt ttttgtaggt tgtccattcc agtaaaatat ctgtgtattc
     4981 gtttgatcat atgccttttt gtcgatagga taatcgctaa tgacaatatc aggtaactga
     5041 tcagcggatg cactatattt tattgggcgt tcagtcatag ataatatcct tttctttgcc
     5101 cataattcct gtgttttacc atactttgat tgaatataga atatgacagg ttgttcgtgt
     5161 tttataaaaa tatccctgac taacatacag tattgaaaaa tcaaccgtct gttcttttca
     5221 taataagtat taagctctgg aattctgcga gctaccccac taaaaaactg ttgcatgatt
     5281 ggatacatca ctggaaatat atgctgaaaa tctctgtctt ctgctgattt tccaaaagca
     5341 tccaccttct tttttgtccc aaaaaccctt gaatacaaat ggatcattgc caaattgaca
     5401 tatagataat ttttttctga agttgtaaaa caaatcttga agtttttttc aaaattttct
     5461 acaatagacg attcaatcca gccccaactt tcttgtactt tatctggagt atccaacaaa
     5521 tcttcttcca aataggtgtt cattacacca aacataaaat aaaggtaagc ttgctctgtt
     5581 ggagtgatgt gattcatcga ccagaagttt gcttcaaaaa aaggctctag tatgtaatgc
     5641 gtaaactctt cttttgttat caatggatgc acaaatttgt tcatctcttc tgggacatac
     5701 gtaatcgagt ggccttgttt cattcgttgc aacgaaattc ttagtaggct gatccatttc
     5761 tttctatcta tactacgcgt atatggcgca tgtttcgcaa atttttctaa tagttgacgt
     5821 ttatttttta tacttgataa tatatcagaa tgattttcgt cgaatgactg ccaatacatc
     5881 aagtgaaaaa agtatcggat acggtattct tctccttcta tcggattacc ttttttcaag
     5941 ctgcaagata aatgatagtt tttcaaaata gtattaagtt ctcttaattt tctttgaatc
     6001 gtacgcaaac ttatataatt ttttgactaa gattttctaa ggattgataa gtacctttgt
     6061 aaagtgaatc cagtaaaata taaccaatcg ttccctctaa aagtatagaa taaactttac
     6121 tcagtaaaaa ctgagaagaa aactcgactt ttatcatctt gttttctgaa agaaaagata
     6181 atttatattc tggatacctt tcgcaaaacg attctgcttg ttgaatcacc gaattcatct
     6241 tagccagggt gtagtttaga tctaaagcga tatccttcac cacatataat ttctcgtctc
     6301 tttcataaca atagttcaaa aattgatata cttgcctttc attgatatca ataaattctt
     6361 caaacaaagt acatactcct ttttatctat tatttagact tattccgaca gctacatgaa
     6421 taacgtacaa atagattcgt tactgatttt tcagagatac taccatgcgc agtgtgcatt
     6481 gtcaattatt cttgctctta ttttccattt gtgttcctgt tatttcctgt ttctcaaata
     6541 tgttagtaaa caaaaataga tctggcaaag ctacaacttc gccaggcaat acagcactct
     6601 cgaatgaaat attcactcaa aaatctatcg ttttattcaa ttttagatct gttgctttta
     6661 ttctttattt cactcgatgg aagagagaac gaaaatagct tgctcctaca atcaaaatga
     6721 gtaacacacc catccatgta attagcttgt tcaaggttct actaccagtt tctggtaaat
     6781 cgctttttct attatctgtt tctttcgctt tttcttgatg atcaattcca cgagaaacaa
     6841 aatcaggctg ttttagcaca ctttttaggt tgctccctgc atttctcttt tgttccttag
     6901 ttggtatgat aggtaccaaa tcaagttcat tatttacttt tcctgtgctg ctttcgctgc
     6961 tcgtgcttcc tgtgctactc tcactactca tgcttcctgt gctactttca ctactcgtac
     7021 tctccgtact gctttcgctg ctcgtgcttc ctgtgctact ttcactactc gtactctccg
     7081 tactgctttc gctgctcgtg ttttctgtgc tactttcact gctcgtactc tctgtactgc
     7141 tttcaccgct cgtgttttct gtactgcttt cactacttgt gctatccgta ctgcttggcg
     7201 gtccaacaac tgtccaactg cttggtttca aattctccgt gatcgtcatc tgtccatcat
     7261 aaaaaacaat caaatcaact ttatctttac tcttgatctc atcaatcgat agttctgcag
     7321 aaatcattat tgtatactca ataccagcgg tccattgtac gtcttttaaa taaacttccc
     7381 caataacttt ttctgaactt ccattaccat cccaaccata tgttagaaca gcatgtacat
     7441 cactagcatt cacgcttttg tttggtttga ctggaatagt aatgacttgt tgtgtcatgt
     7501 cataaacaat ccgatcagtt ttcacagtaa atgaaagatt ctcatttgcc cgttcatttg
     7561 cagaagtact gattccaatc attgctgcaa ttatactcag cccaagaata acaaataata
     7621 cggtcgctgt tttcttcatt tctatcactc tccatataat accttctatt tgataaagga
     7681 attatataaa atgaaaatga tgagaacacg acagtcagtt gtgaaaatct aacaaaagat
     7741 gtcgtgtatt cgcttgaatt tgtttacttt ttcgtttaat caatgacaga cttaaaaaag
     7801 caagaccgat atcagccttg ctttcttcat cttttattga taaacagcag tgtatttctt
     7861 catccgcttt gttttatatg catagagtaa caaaccaaca atcaagacta acaagaataa
     7921 tgtagttgat atagatgctt cagccccaaa gtttccccca gtaattattg tattttgcga
     7981 ttggatcaga gtggagaaaa aaccttgttc ttgcgttccg ctgactggta tacccaaaat
     8041 agctccttgt gtccaattcc aagtaatatg agcaccaata gccgtaaata aacttcctgt
     8101 actgtagata agtgcgctga aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa
     8161 tgtcatgtta ggattcaatc cgtgcaaaag agcaaataat gcagcagatc caatcattgc
     8221 tattaacgga ccaaatctaa gcatctgatg ctgaaaaaag ccacggaata aaagctcttc
     8281 tgatgccccc actgcaataa ataaaataaa actcgttatc agcggcacaa tgtttgcgtt
     8341 tcctgaatct aaccgatacg cccccgtcaa atataatgga ctagtaataa gcagaaacac
     8401 tccaattcca acaacgagac ctcccgctaa atacccccag tcttttcctc gaacagaaac
     8461 accataatct tctattcttt ttccttcgac cttactagca agaaataaag ctagtaaaaa
     8521 aacagctaaa tgtccgaccg taccggcaat tgctccaatc cattcactat ttccaataaa
     8581 catatccgca agtgcattat tgacaagaat tcctaaaaca aaaccaacta agactattaa
     8641 aacagatcca atcaaccgaa taatcctgat catctttcat ccctccttta tattaagttt
     8701 aacaaccctt ttctttcttg tctcgtgaaa actatgtaaa tgttctccat catagagaga
     8761 atagattttt acgaactaag aatatctaca gcaaccttac gaaaatgaaa gactgtctcc
     8821 ctaattcttc tgttagactg tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca
     8881 actactaatt atgtgccttt cagcatgtgc aattttacta ttctcaggtt gtagcaagtc
     8941 caatgaaacg attgcaacgt ttaaaggtgg aacacttaca gttcaggatt tttatgataa
     9001 cccaaaagtt aaatcaaata atcaaacact attaaaaaaa atgattgtct tcaaagcatt
     9061 tgatgatgta tacggaaaag agatttcttc aaaggaagta acaaaagaat ataatgaaca
     9121 aatcaaaaaa ctcggtccaa actacaagga acaattaaaa gcagttggcc aaacagaaga
     9181 gacatataag aaactcttta aacagatgct ggctttccaa tacggtttga aagccaatgt
     9241 aaaattaaca gataaagatc tagacactgc ttggaaagaa ttctatccag aagttagtac
     9301 acagatcatt ctattttcga ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga
     9361 aggagaaaat ttctccaagc ttgttcaagc ttatggaaaa aataaaacat taaaggaaac
     9421 tgatggaaaa atgaattttg attcaaccaa tccagagatt cctactgaag taaaaaaagc
     9481 agcatttaaa ctaaaaaacg gggaagtttc tgatatcatt cctgtaacag atccaacgac
     9541 ttatcagcaa agctattatc tagtaaaaat ggtaaaaaaa caggataaag gttctaataa
     9601 agacaaatac aaatctgagc tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga
     9661 attcatggat aaaacaatcc gtaaagtgat gaaaaaagac aatgtaacta ttaaagatcc
     9721 ttatgtaaaa aatatattca aataaacaaa tttgacccaa ttggggatgt ggcaaaattt
     9781 gagaagtgat ttttggaaat tccttcttat ttttgaggca aaccactttt gccacaactt
     9841 catcggggcc tttttttatt tgtaccaaaa caaatcactg gatttgttac aatcatgagg
     9901 atataattta aaaaagtttt ttatttctat cacatgaatc aatgcgccat taagtagatt
     9961 ctttcatgct tccctatttt ttcacttgcc gtatcgcttc aaaaacaact atcataaact
    10021 caaactcgac tgaaaagtta ttttaaaaaa gactaaaaat atcccttctg gacttgttct
    10081 tcttattctg ttatactaga gaaacacgaa agatattttt atgcgacact tttcatttgt
    10141 ttttatgaaa tttattcaaa actggcgtaa cttcagagtt acaaggatgt attagaggtt
    10201 agggaataca tcgtttaaat gagaaataag taatagttag taatagtcac ccaaacttta
    10261 aattaatgaa tattgaatcg tcagatagcc tcctcgagtt ttcgagaagg ctatcgttta
    10321 ttttatggag aaattgattc agaagtaatc agcgaccttt tttagttcac caatcgtttc
    10381 tgcttggaaa gtttgcaccc aatcttcttg gaagcccctc actgctttat caatcgaggg
    10441 catctctaat acttgatcta gtagctcttt acctaacgtt atcgcatgag ctccagatcg
    10501 aatggctgta gtgacttgtt cacaattttt gaaactagct gctaatattt ttgttggtct
    10561 ttgcgtacgt tcgatctcag tccgcaacga atcaatcact ttcatagcat caatgccttg
    10621 attttgcatc ctgttataat aaggtgcaat ataatctgct ccattattaa tagctaaata
    10681 cccttgtaat tctgtgtaaa tagctgtacc ggtaatcaaa taaccttctt ttttcaactt
    10741 tttgatcgct tgaaggcctg cttgattcac cggcaccttg ataaacgtat cttttcccag
    10801 ttcttctatg ataactcgag catcctctat catttcttct tccgttcttc ctattgtctg
    10861 aatatggata ggaatctgtc cgattatttt tttgatttct ttcatatgcg agaagaagtt
    10921 tgacgctcct tcttttttca gaattgtagg atttgttgtt acgcctgtta aattcaaaaa
    10981 ctgttgacaa gaataaatac tttttgtatt tgctgtatca agcatgattt ccatatagct
    11041 ttcccccatt ctgatcctct tgatcgattt taatgattgt cccctttctt aaatgcggca
    11101 tttccttatc ttctacatac attgttcctg gaagttccgg ctcatgagat ccatcaaatt
    11161 ttatcgtgat atgtcctaat tcattcaaat tagtttggac aacatctcct actgctgtga
    11221 ttacatacat ttgtttatca aaacagagct tcatatcaca agttatttct tttcttacct
    11281 gattcaattc aatcttataa caataatctg ttaatgtttc cggtgcttca tctccaaata
    11341 aaatcattat tttttctttc ctgaatactt ctgcttctat accaacatct gttacaaccg
    11401 tttcaaagat cgtcatc
//
LOCUS       DAZKCY010000080        11119 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000080 DAZKCY010000000
VERSION     DAZKCY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.081"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..1340
                     /gene="lysA"
                     /locus_tag="ACG8XC_001958"
     CDS             81..1340
                     /gene="lysA"
                     /locus_tag="ACG8XC_001958"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP9191647.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1327..2127
                     /gene="dapF"
                     /locus_tag="ACG8XC_001959"
     CDS             1327..2127
                     /gene="dapF"
                     /locus_tag="ACG8XC_001959"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP9191648.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2130..3011
                     /gene="dapA"
                     /locus_tag="ACG8XC_001960"
     CDS             2130..3011
                     /gene="dapA"
                     /locus_tag="ACG8XC_001960"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP9191649.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3008..3724
                     /gene="dapB"
                     /locus_tag="ACG8XC_001961"
     CDS             3008..3724
                     /gene="dapB"
                     /locus_tag="ACG8XC_001961"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP9191650.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3730..4425
                     /gene="dapD"
                     /locus_tag="ACG8XC_001962"
     CDS             3730..4425
                     /gene="dapD"
                     /locus_tag="ACG8XC_001962"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFP9191651.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4441..5442
                     /locus_tag="ACG8XC_001963"
     CDS             4441..5442
                     /locus_tag="ACG8XC_001963"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9191652.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5439..6800
                     /locus_tag="ACG8XC_001964"
     CDS             5439..6800
                     /locus_tag="ACG8XC_001964"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFP9191653.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6760..7911
                     /locus_tag="ACG8XC_001965"
     CDS             6760..7911
                     /locus_tag="ACG8XC_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9191654.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7886..9055
                     /locus_tag="ACG8XC_001966"
     CDS             7886..9055
                     /locus_tag="ACG8XC_001966"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9191655.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9230..10609
                     /locus_tag="ACG8XC_001967"
     CDS             9230..10609
                     /locus_tag="ACG8XC_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9191656.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10890..11119
                     /locus_tag="ACG8XC_001968"
                     /pseudo
     CDS             10890..11119
                     /locus_tag="ACG8XC_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3700 a         1945 c         2493 g         2981 t
ORIGIN      
        1 tattattcca ataaaattca aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa
       61 gtgaaaagag gaatgaaaaa atgacagaat tgataattgg cggagtcaca gcaagcacat
      121 tggcagaaaa gtataagaca cctctgtatg tgtatgatga agaaaagatg agacaaacga
      181 tgctgtcatt caagcgaggc tttgtatcga aacgttttga tacaaaagtg ctttacgcct
      241 ctaaagcatt ccaaacgatt gagatgctgc atttagttcg tgaatatgga ttcgggctag
      301 atgttgtcag tggtggggaa atctataccg ctttgcaagc tgattttcct agagatacta
      361 tctacttcca tggtaacaac aagacgccag aggaactgat ctatgcactt gaaaatgatt
      421 tgttgcatat tgttgccgat aatttgatgg aagttgaatt attagcagaa ttaagccaac
      481 gctatttaaa aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa gcacatactc
      541 atgaatatat cgtcactgcg catattgatt caaaattcgg catgagctat gaaagtaaag
      601 agtgccaaca agcccttcag ttgataaaag aaagcagcta tctagagtta gaaggatttc
      661 atgctcatat cgggtcacaa atttttgatg ttactgcttg gttagctgaa atcgacaaat
      721 tgacgaatta cttaaccgat tttgaccaac ctctttcctt gaatcttggc ggagggtttg
      781 ggattcgtta tacacaaaat gatcagccga tcccagtcga agaggcacta aaaaaactga
      841 ttgaatatac ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg
      901 aaccgggtag aagtattgtt ggagaagctg gtacaacact atacacgatt ggtttcatca
      961 aaaagacacc acataaaaaa tattactttg tagatggcgg gatgacagat aatatccgtc
     1021 cagctctgta tcaggcagaa tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa
     1081 cagagaaagt gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag
     1141 cttatttgcc gaaagcagaa tcgggtgatc ttttagctgt ttattccacg ggagcttatg
     1201 gatactcgat gagcagcaac tacaatcgag cgacacgacc agcagttgtt tttgtcaata
     1261 atggccaatc aagactgatt gttcgcagac aagcattttc tgatctacta aggggggaag
     1321 tttcctatga tccttcataa atataatggc tgcggaaatg attttatctt attagattac
     1381 gcagaagaca cagattatag tcaattagcc gcttctttat gcacaaaaga aaaatatgat
     1441 atagacggat tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta caacaaagat
     1501 ggcagccgcg cgccgatgtg cggaaatggc attcgctgtt ttgctcgata cgtaaatgat
     1561 cagaacatga tgaatgaaca ggaattcaac gtagagacat tagcaggcac aagaaaaatt
     1621 acggtgttgg atccctcacc tttttattgc agcgtagata tgggagtgcc agatgattct
     1681 ccggaaaaaa tcaaagctaa tcaaacttat ccaataaaaa accaggaaat agagatcaat
     1741 ggtgaaaaag taacgatcac tagtttattt ttaggaacga ttcataccgt tgtttttgtt
     1801 gaaaatgcgc gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca tcatcctttg
     1861 ttcaaagaaa aaaccaatgt caactttgta gaagtagtca atgaacaaga attgatcgtc
     1921 cgtacctatg aaagaggagt aggttggaca cttgcttgcg gtacaggttg ctgtgcctct
     1981 tatgtggttg ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt tcacttggaa
     2041 caaggagact tgatgatctc tggtgaggat gcgatacaaa tggctggccc agcagttcat
     2101 gaatggacaa gaatattgga ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca
     2161 cgccgatgaa tgaagaggga tcagtagatt atgctggttt ggaaaaattg atccaattcc
     2221 atttagatga acaaacagat ggattattag ttttaggtac aacaggagaa tcttctacgc
     2281 tcacacaatc tgaggaggag cagatccttc aactgacagt aaagaaagtg gctggccgtg
     2341 tgccggtgat tgcaggagct ggaacgaata atacgaaaga aacgattgaa aaagcgaagc
     2401 attttgcttc tttgggggca gacgcacttt tggtcatcac cccttactac aacaaaacaa
     2461 gtgatgcagg actagctgct cattttacag caattgcaga agcctcaccg attccgctta
     2521 ttttgtataa tgttccttct agaacaggca tgtcgattcc aatccatgta ttagtaaatt
     2581 tagcagaaca tcctaatatc attggtttga aagaagcctc aggagatatg gcttatgtga
     2641 tggatgccgc tcggttgatt ggagaagaat tcttccttta cagtggaaat gatgatttga
     2701 tcctaccagt aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg aatatccaac
     2761 ctaaaatagt ccatgaatta gtaaaagata cgcaggacgg aagatggcag caagcaaaag
     2821 aaaagcaatt aaatgcactt gaactgatcc atgccttgtt cagtgagacc aatcctattc
     2881 ctgtcaaagc agccatgtca ttattagact taccttccgg acctctacgc ctgcctttag
     2941 tttctctttc aaaagagaaa aagaaacaat tagctcagct tttgttaaaa gaaaggacgg
     3001 ttgaaaaatg aaaatcggac tgattggcag tggacagatg ggaaatagag tcgaagaagt
     3061 tgcacaagaa agaggagata cggtccttct tttacggacg gcacctaaag aagcaactac
     3121 gcttgctttt acggaactgc cagaactgct tattgatttt tctcatccag ataatttaga
     3181 gaaaatcctt tcgtatagct tatcatatca acttccgtta gtcattggca cgacaggtta
     3241 tacacaatca cagttccaag aaataaaaga acatgccaaa catgtacctg tgatgtatag
     3301 tgctaatttt tcgttcggca tcatggtgat gaatcaacta atcaagcaag cagctgcaat
     3361 gttgcaaggc tggcagatcg aactgattga aaaacatcat agtcgtaaaa aagatgcacc
     3421 atctggtact gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt
     3481 atacaactgg caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc
     3541 aggttcttta cctggtgaac atgatgtatt gtttgctgct actgatgaaa tatttacgat
     3601 caaacatgaa tctttttcta atcgaatctt tgctgcaggc gcagtagaag cagcggactg
     3661 gttgaaagat cagtcaccag gttattacaa attagaagat ttattaatgg acaaaggggt
     3721 gtaaaaaacg tggaagctca agaaatcatc cgctacattc aaacggcatc taaaaagacg
     3781 cctgtgaaag tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt
     3841 ggtgaaggaa gcagcttcgt tgtttttggt gactatgaga cgattgctcc ggttttagaa
     3901 gctgaatcag aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt
     3961 cctttgctag atattaaaaa aattaatgct cgtatcgaac caggtgctat tatcagagat
     4021 caagtgtcaa tcggcaacaa tgccgtgatc atgatgggag cgattatcaa tattggtgca
     4081 gtaatcggtg aaaacacgat gattgatatg ggtgctgtct taggtggacg agcgacagtg
     4141 ggcaaaaact gccatattgg tgcaggagct gtcttagcag gtgtgatcga accagcatcg
     4201 gccaaaccgg ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt gattgttgaa
     4261 ggtgtccata tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg
     4321 gcagcagaaa ctgttgtcgg tggaatacct gcacgtgtat taaaaataag tgatgatcag
     4381 acaaaagaaa atacagcatt gattgctgcg ttacgcgaat tatagaggag atggagaaaa
     4441 atgcgcgaaa tagatacctg tattgtgggg gcaaccggat tagtcggcca aacgatgctc
     4501 aaagtgttag aagaatatga ttttccagta ggacgattga aattattagc ttcaaaacgt
     4561 tcagccggta aagtttgtgt attcaagaaa aagaaacaca ctgtacagga attgacagaa
     4621 acttcatttg aaggatatga tttagcgcta ttttctgcag gtgcttctgt atccaaagag
     4681 tttgcgccaa tcgcacgaag tcagggacta tttgtcatag ataattcctc agcatggcgg
     4741 gaagattcgg atattccatt aatcgttcca gaggtcaatc tggaagacta tacattgaat
     4801 cggctgattg caaatccaaa ttgttcaacc attcaagcgg tacttccgct taaagcttta
     4861 caagaagcct ttggtatcca gcgagtcaat tattcgacct atcaagcagt ttctggttcc
     4921 gggcaaaaag gcattgaaga tcttcatcgt acgcaaaatg acgaggaacc agttttgtat
     4981 ccttataata ttagccaaac ggtgattccg gaaatcgatc agcctttgga aaatgggtat
     5041 acaaaagaag aacagaaaat gataaatgag acccgtaaga ttctgcatca gcctgctttg
     5101 ccagtatcag caacctgcgt acgtgtgcca atcgaaaatg gacatggagt ctctgtggca
     5161 gttcaactgc aaaaagagtt tacgacggaa gaaattagag agtgtctcag ccagtttcca
     5221 gggattcaat tagaagatga tctagcagca cataaatatc cgacgagcat tcttgctaga
     5281 ggaacagata tggtttatgt tgggaggatc agaaaagaca cttctttaga gaacggattg
     5341 ttgttctata cgactgccga taacatacgc aaaggagccg ctgcgaatgc cgtacagatt
     5401 gctgcagctt taatgcagaa aatagcggag gtggcaagat gagtaatacg ctgaaagtag
     5461 cgaaattcgg cggaacatcg atgtctcatg aagagcaatt caaaaaagtc cgtacaattg
     5521 ttctttcaga tcccaatcgc caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag
     5581 atgatgacaa agtaacagac ttactttatt tgatttatgc ttaccttcag catcatgttg
     5641 attggacacc cctttggaaa aggatttgtg atcgtttcat tcaagtaaga gatgttcttg
     5701 ccctgcagtt acctattgaa gatgaactag cacgcataca gaaacaactg gaagacgaaa
     5761 agataactga agattattta gtaagtagag gtgaatatct gactgctcaa ctgatggctg
     5821 agtatcttgg ttaccagttt attgacgcaa aagacctgat tttctttgac tatgatggga
     5881 aaatcgatca ggaaaaaaca gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa
     5941 tggttgttcc agggttttac ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg
     6001 ggggaagcga cattaccggc gcgatactgg cgaagatgtt gcaagcggaa agttatgaga
     6061 attggacgga tgtttcagga atcatgatgg ctgatccgcg aatcatcgat catcctaaaa
     6121 tcatcgagga aatctctttt agagaactga gagagatggc ttatatgggg gctaacgttc
     6181 ttcatgaagc agctgttttc ccagttcaag aggctgatat ccccatccaa ataaaaaata
     6241 ccaatgatcc tttcgctcaa gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg
     6301 gactgacagg aatagctgga cgcaaggatt ttctttccat cactttattc aaacgtcata
     6361 tgtccgatga aatcgggttt atctggaaag caatgagcat ttttgctaaa catgagatat
     6421 caatcgaaca tattccttca gggatcgaca atatcggagt gatcgtctca gcagaagcga
     6481 ttgccgataa gttatttctc atcacgaaag aattaaaaga aacacttggc gtggaagaaa
     6541 tcgtcgtgat cgaagactta gctttgatct ctgtcgttgg gggtccgcat aaagaattga
     6601 ttggattatc tgggaaagtg ttatcgatat tgaacaaatt agaaattcgc acatcgatct
     6661 tatcacaagg agcacaggag ttgaatctga tcatcggtgt accgaataat caatatgaaa
     6721 cagtagtgaa gggtatctac gaagggatgg tgaatacgga tgctgaaaga atatcgaatg
     6781 gcactccatc aaattcctga aatcgggttt catgaattca agacaaaaaa atatttatat
     6841 gaacaagtaa aaaacagcga ggccgttatc catgaaatcg gcgaaacagg actactcctt
     6901 tattttgata atcagcaaga agaaacgatt gcttttcgaa cagatatcga tgcactgcca
     6961 gtcaaagaag aaacgaatct ctcttttgct tctaagcatg aaggttatat gcatgcatgt
     7021 ggtcatgacg gacatatggc gatgctttta gggttgactg actttataac taaacacatt
     7081 gaggatctta tttataatgt agtactgatc tatcagccat cagaagaaat ttccggtggc
     7141 gcacaaagcg ttattcagtc aggattgttg gataaatatg aagtacaagc aatatttggc
     7201 tttcatctat ggcctggact agctaaggga gaagttttct ctcgaccagg acctttgatg
     7261 gcacaaagca gtgaaacaga cattatcatt aaaggccagt ctgcacatat tgcttctagt
     7321 gaaaaaggaa tcgatagtct ggaggcagct gtacgatttt tgaagaatgt ttatgatttt
     7381 gaccggcaat taccggcaga gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga
     7441 acgatccgaa atgttttagc tggagaagtt attgtatccg gaagtatccg ttcttacagt
     7501 tcagatatgc aaaattattt gaagagggga ttagaccatt tagcagaaca gtttcactct
     7561 acagctacta tttcttttcg ctataatgac ggctatccag ctgtaatcaa ccatagaggc
     7621 ttatatgcag cactgttaga ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa
     7681 gccgaagatt ttggcatata tacgcaacaa tatccatgtg tatttttctt tttaggtgtc
     7741 ggtgataccc ctgctttaca tgatccgacg ttcgattttg atatggatgt tttagaaaat
     7801 ggtgtagaat ggtacaaaac gattcttttt acagaaaatc ttcttgtaaa aatcgatagc
     7861 agttcaaaag aggagcgagt aaataatgat gaatttatcc aatcgattta gtcctaggct
     7921 ttctagaata gaagtatcaa aaatacgaca attcgatcag caaatatcgt cgataccaga
     7981 tgtgatcaaa ctcaccctgg gagaaccgga ttttccaact cctgaacatg tcaagcaagc
     8041 aggaatagca gcgattgaag aagacttttc tcactatacc ggaatgagag gattagagga
     8101 actgcgggaa gcggcttgta tatttcagca acagcgttat ggattaactt atgatccgca
     8161 aacggaagtt ttgacgacag ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt
     8221 tttggaagaa ggagacaagg ttcttattcc agcgcctgct tattcaggat atcagccatt
     8281 agtggaacta gctggtgctg aattgatccc gatagatact tctgacacag ggtttgtctg
     8341 tcagccggaa caatttgaac gagcatttga acaatatgga tcagctgtca aagcagtcat
     8401 tttaaactat cctaacaatc ctactgggac gacattgtct gcaaaacaat tgaaagcaat
     8461 cgctgaggtt ttaaaaaaat atccggtatt tgttataagt gatgaagtat atgctgaatt
     8521 gacttatagt ggaacgcata tgtccatagc tacttatctt ccagaacaaa caatcgtgat
     8581 aagcgggtta tccaagtcac atgcgatgac tggttggcga gtgggcttta tttttgctca
     8641 aaaagccctt attgacgaac taattaaagt ccatcaatat ctggtgactg ccgctacaac
     8701 gatgtcgcaa aaagctgcgg ctgaagcgct aataaatggg gtgaaagata gcgagaatat
     8761 gaaagagcaa tatcaaacac gaagagatta tctggtccag caattggcgc ctttaggatt
     8821 tgaagtgacc cagccaaacg gtgcatttta cttgttttgc aaactcccag ctgccattca
     8881 aactgactct tggcagtttt gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc
     8941 aggaattgca tttggcccag aaggagaagg ttatatccgg atttcttatg ctagcagcat
     9001 ggaaaaatta catgaggcct gcaaacggat caaagacttt ttaacagaac agtgattcat
     9061 agattttatc aaaaaaacaa tcgttaattt tgcggtatat tctgcaagga gacgattgtt
     9121 ttttaaattt agttcaaatg aacgcgtttc ttttgattca taactaatgt aatgttaagt
     9181 ttaaattgtt tagaatgatt ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat
     9241 caaagttgtc attgtaggtg catctcatgg agggcatcaa tcgatcttgg aactattgac
     9301 acgatacggt gaaaatgtag atatcacgct atttgaagca ggagactata tttccttcat
     9361 gtcatgtggt atggagctgt atttagaaga caaggtaaca gatgtaaatg atgtacgtaa
     9421 tttcagacca gaaaactttc cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa
     9481 aacgatcgat gccgataaaa aaacagtaac ggttactagc gcatcagata acgaaacaaa
     9541 agaatatcct tatgacaaat tgattttgag ttctggtgtc aaaccaaaca atctgccagt
     9601 accaggagca gatctagaaa atgtctactt gatgcgtgga tacaattggg caaccaaaat
     9661 caaagaaaaa ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg cgggttacat
     9721 tgggatcgaa gcagcagaag caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt
     9781 gatcgataga cctttaggca cttatttaga tgctgagatg acagatattt tagaacaaca
     9841 cctaaatgaa aacggtattg aagttttgac aaatgcaaaa atcgaagctt ttactggtaa
     9901 cggaaaagtt gaggcaatca aaacaagtga aaaagaaatc ccagcagatc tagtcattca
     9961 agctgccggt gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact tagatgaacg
    10021 tggctggatc caaacaaatg aatacttgca aacgaatctt ccagatgtgt atgcagtagg
    10081 agatgctaca cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac
    10141 tgttgcacgt agagaagcac gttatgtagt aaaacatctt tttgaagaag taccaaacaa
    10201 accgttcggc ggagtcgtag gttcctctgc attaagtgtc tttgactatc attttgcagc
    10261 aagcggattg aatagcttta cagcagcaaa agcaggagta gatgtacgag tgtcttatta
    10321 tgaagatacg ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa aagtgtccat
    10381 ccaattattc tcggatccaa tgacccatca attgctagga ggagcagtat tatctaccta
    10441 tgatgttaca ggtcaaggaa atgtattggc actagcgatc caaaacaaac tgactttaga
    10501 agaactagca gaagctgact tcttcttcca gccaggcttt gaccgacaat ggagcatgtt
    10561 gaatctagcc gctcaacaag cgttaggaga agaaccattt gtagaataag aaaacgaaat
    10621 atttgaaaag aggttgtgaa aaagctgcct tgcatcaagt aataagaaca gccaagtaaa
    10681 aacagcttct tatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac
    10741 accgtttatt ttcaaaaaag ggactgtgac acgatttgtg taccgacccc caaaagttag
    10801 accaaaaaat ctaacttttg gaggtcggta ttttttatgg ttaaatatac ttttgaactt
    10861 aaattaaaga tcgttcatga ttatttggat ggcaaaggcg attggaccgt aaaaatccag
    10921 atcaagagat tgaagcggaa atgcttaaaa ttcgggagaa gcacaaggat tatggctgtt
    10981 tacggatgac caatgaacta agaaaccgcg gtttctctat caataaaaag aaagttcagc
    11041 gcttgatcaa gaagctagga ataaaagtaa cggcatacac gcgtaaatca cgtcgctaca
    11101 attcttatcg aggaaaagt
//
LOCUS       DAZKCY010000081        11105 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000081 DAZKCY010000000
VERSION     DAZKCY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.082"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            375..1814
                     /locus_tag="ACG8XC_001969"
     CDS             375..1814
                     /locus_tag="ACG8XC_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9191657.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2109..2327
                     /locus_tag="ACG8XC_001970"
                     /pseudo
     CDS             2109..2327
                     /locus_tag="ACG8XC_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            2576..2791
                     /locus_tag="ACG8XC_001971"
     CDS             2576..2791
                     /locus_tag="ACG8XC_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191658.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3008..3264)
                     /locus_tag="ACG8XC_001972"
                     /pseudo
     CDS             complement(3008..3264)
                     /locus_tag="ACG8XC_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3451..4362)
                     /locus_tag="ACG8XC_001973"
     CDS             complement(3451..4362)
                     /locus_tag="ACG8XC_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9191659.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4381..5385)
                     /locus_tag="ACG8XC_001974"
     CDS             complement(4381..5385)
                     /locus_tag="ACG8XC_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFP9191660.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5382..7505)
                     /locus_tag="ACG8XC_001975"
     CDS             complement(5382..7505)
                     /locus_tag="ACG8XC_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP9191661.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7510..9957)
                     /locus_tag="ACG8XC_001976"
     CDS             complement(7510..9957)
                     /locus_tag="ACG8XC_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9191662.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9944..10333)
                     /locus_tag="ACG8XC_001977"
     CDS             complement(9944..10333)
                     /locus_tag="ACG8XC_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9191663.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10393..10896)
                     /locus_tag="ACG8XC_001978"
     CDS             complement(10393..10896)
                     /locus_tag="ACG8XC_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9191664.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10909..>11105)
                     /locus_tag="ACG8XC_001979"
     CDS             complement(10909..>11105)
                     /locus_tag="ACG8XC_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191665.1"
                     /translation="WFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLV
                     FNASGVKDVLLSLFNRIVGA"
BASE COUNT         3239 a         2334 c         2000 g         3532 t
ORIGIN      
        1 tcctatggaa ttgaatatca tagcaatgta tttgaaaagc aaattggaaa acaaataggt
       61 gcaacaccat aaatatgaaa aaattgggaa aaagtagaat ggaaatttgc ttacgtctca
      121 cgagatagct cacactatta atctgaagaa caatgaagtc gacttttaga aattgctaga
      181 agaaagtaaa gcgaattatc actagttact atagagtaag ttcaaataaa gcatctccaa
      241 cttacatggt tacatgagga tatcaaatcg aaattactca agcttcctat ggctagagtc
      301 gcgagaagtt gttgggcagt agacataggt tatattgttc ttagctacac gatcattaga
      361 aaggaaatcc tattatgtat agttatcaag aagatgcaaa agtccaaaaa aatcgctggt
      421 ggattttgat tgctatatct atgtttacct tcatgtccac attggatggc agtattgtca
      481 atattgcact acctaccatc tcagatgata tgggcatacc aatgaatcaa gcggaatggg
      541 tagtgtccgt ttatctgatg attgtctgtt cttgtttgtt gctgtttggt aaaattggcg
      601 atagccgtgg gaaaatcaaa gtatttcgga ttggtacgct gatctttgtc ttgggatcgc
      661 tgttgtgtgg gttcaatcat tcgctaggta ttttattgtt cgcacgggtg gtacaagcca
      721 ttggtgctag tatgactatg gctacaaatg ccggaatcat cacggaagtc tttcccatac
      781 atgaacgtgg tcgggcattg ggagcaatag gtgcgtttgt ttcattaggc tcgattgctg
      841 gaccaggtct tggcggcttg attttgtccc aattttcttg ggcgtacatt ttttggatca
      901 atgtaccggt gggaatcgta acgatgttga ttgctgaaaa attcttacca aaagatatca
      961 ccatgagcgg ccatacaatc gatggagggg gctttgctgc cattgcggct acgattatca
     1021 gcttttttgg agcaatcttt ttggggcaag aaagtggctt tttccatcca gtcccccttg
     1081 tactgttcgt aatttcagtg attgcctttg tcagcttttt aaaagttgaa aagaaagcag
     1141 cctctccatt gatttctttc ggtattttta agaacaagat ttttaccatg agcttgatta
     1201 cagccttgtt gattttttct tccaacttct ttgtcaatgt agtgattcct ttttatctgc
     1261 aaaatgctcg cgggttgagt cctagctatg ctgggctgtt gatgatggtc tttcctttga
     1321 tgatggtcgt cggggcgcct ttaagcggct acctgaccga ccgggttggc ccggagattt
     1381 tggtattagt aggcttgggc ttattgtcgg taacccaggt catgtatatg tttttgtcag
     1441 tggactctcc tttgtggcta tacattgtcg ctactgccat catgggtgcc ggcaatgcct
     1501 tgttccagtc ccccaataat acgatggtta tgagctcggt cactatcgaa aacttgggac
     1561 tggcaggctc gatgaattcc tttgcccgaa atttggggat ggtcagcggg atttcgcttt
     1621 ctacgacggt cttatatcaa gcgatgtctg ctaagatggg ccagcaagta actaactata
     1681 tcgcaaatcg gccggatgtc tttttatatg gaatgaagat taccttttta ggcagctttg
     1741 ttttgtgttt tgttgccttt ttcctgacta tctggcgtat caaaaaacaa ggaggtattc
     1801 agaaaaatgt ctgaaaaaac cactgttgaa gaatttttgg aggcctgcgt atgggtgagt
     1861 gggatttcca tggatgatgg aagatgaagt gaacttctaa aagccgaaaa actcaagtat
     1921 tatcttcaaa atactagtgc tcaatgagat gcttgatttg atcacaagaa gactgcgaaa
     1981 gccgctggca accgcaagct aggtatccca ccgatggact acgttttaga ggctactggt
     2041 tatgaatacg gagccaacgc tctcatctgc ataagaatac atcacccaga ttatgtaaag
     2101 attttcgata cttctgtcaa aaaatcatta agatagataa atgatttccg tctagtcaaa
     2161 ttggttcaga atgtagacac acagatggta agaagtcact cacaattcga gaatggactt
     2221 gtcctatttg ccgtatacat cataatcgaa acgtcaacga tagtatcaat attttgacca
     2281 agggctatga atacaagcat tgccttagaa aacatcaaga accttaggaa caacggggat
     2341 agcttggtta ataagagata ccgctgttta ggcttgcaag tatactcttt tatcaagaat
     2401 tccgtggctt tagtcatgag aggcttaata ctatgaaaat tttctttagc atttttgatt
     2461 taggttcctt ttctaattat aataagatga ccactacagt aacctactga aatatataaa
     2521 cagccactgt agtaccattt tcaaagtagt ctatgagagg agaatcttca ttcttatgaa
     2581 cgctggaatt gcggatatga atttaataaa aaaaaccctt aatgatttta cttccaattc
     2641 aatatcaaaa ggtactggta tcaatttaag cacaataaaa aagctgaaaa gtggtgaaag
     2701 atcagtggaa aagctgaatc ttttagatgc tataaaaata acggaatttg ctatgaaaaa
     2761 tggaaaagct gagattgaaa tatggagata attatcgtct agtgaattac ttgccactta
     2821 gctgaattta acgatccttg gtgcttgaag ggggagattc ttgggaatag agcatacttg
     2881 caagcctatg tctttactaa caacgatatc tcttattaac caagctatcc ccgtagttct
     2941 aaggctttta cttttatcta agcaataatc gtattcaaag gaaatttttg acccttagaa
     3001 agtctcattt tatcagacgt gagcttaggc attgctgaat tttgttaatt cggttttagg
     3061 ttgtgctttg agcataatat gaaagtattc tttatcacga tttcattcat ctaaggtaat
     3121 gtgatgtgat tctgaaattc tttcaaaggc agtttttgca taagctgaca ttacatcatc
     3181 aatgacatgt ttacaatgtt tcactactaa aataaggggg taaccaagaa gaaaaactga
     3241 atgattattt gtatctaatt ccatggtata tcaaccattt cctatataga ctgattatga
     3301 catggattaa aataaaaagg cactatgtct taattgtcgg ttttcgagtt accgaatggc
     3361 attcatcacc cacctataga ggatgggcga cttctgcctg aaataagtta cactacttat
     3421 aacttctaat aaaattagat tgagtcatta ttatttaaca tcaacgattt tccagtttcc
     3481 atttttttgt aatactaatt tgtactgaaa gtagctagtg gtttttgata cattatcaag
     3541 gtattttatc tttgtactca ctagaaaatt ttttccgttt tgtagataaa ctggattctc
     3601 taactccgaa aactttaagt caatattgat tggtcgcatc gcaccatttt caacataata
     3661 ctccaattct ttttcagaag ctgatggata aagtttaaaa aatgtttcta aaaaatcggt
     3721 gatttcttcc gttgttttgg catcaatttc tgaatcgttt tctacttgtt ttgcttcata
     3781 ttttgccttg tctggctttc caatcattgt tggcaaacta gttactaaat gattcctgtt
     3841 ttcatcttca aagattgtca ctcgatatgc agaagaaatt gattgcgtct tttttccctc
     3901 tgtaatatgt tgctccacta aaaatgaaac gacaaattct tccgagtgtt tttcaacatc
     3961 tagaattttt acagattgaa cttcggaact cgtgggaata tctgctcgaa ccatatcttg
     4021 gattaaagcg agaccgtttt ctgtaaggta ctgttcgaga ttactcattc tcttttcaat
     4081 tgcttctttg ttatttttcc atgaaaaata ttctttcgca aaatcctctg taaaactttc
     4141 aacgccagaa gtattcacta ccttctcttt tataaccact ttttcatgag ttgtatgttg
     4201 atcaatggcg gtgaaatttt tataaacgcc aaaagcaaga ctacaagaca ataaaagcca
     4261 aagggcaagc accatttttc gatgcttacc cacggataca actcgttctt ttttcttttt
     4321 tgggctcttt tccttttgat tccgttcaat ttttatcttc attctttttc ctcttttctc
     4381 ttatttgtat cggccagctc cgataagatg ctgccgccaa tagttttccg ttagattcgt
     4441 ccaccccact ggatctcctg catgaaacat gcgattctcc cctgcgtaaa tgcccacatg
     4501 ggtcacataa gtaccggcat cataggttga atgaaagaaa actaaatcac ctggtttggc
     4561 atcttttaga tcaatatgag tcatggcatc atattgatcc tgagccaccc gaggaagttt
     4621 caggtcaact ttggcataac accattgggt caagccacta caatcaaacc ccgtttctgg
     4681 acttgagcca ccaaacacat aggccgttcc ttcatactta aatgcctcat caaaaatttc
     4741 ttggactatt ttgtcatcaa actgagttgt agttaggtat tgcttcacta ataaacagta
     4801 aaacatattg ccataattat accgccaccc accattttcc ttaatcgcga ttgggttggg
     4861 gtaatcgact ttttgaccac ctgatttttc ctttgaaaaa gattcagcta aagcaaaact
     4921 atgcttcttc ccgtttttcc cgacatgatc tagatacccg ccaccatagt tataggcctg
     4981 aataatacta tcttgatcac accctagttc tttggaacgc ttaaccaaag acgcaaaata
     5041 gagacagcct tgtttgatgg atgcctcggt acttaatgaa ttaggtggta atccggctga
     5101 ctcagaactt tgcataacgt cttccattgt gccaccagat tccacttgaa taatcgccaa
     5161 aagaattgga atatactctg ttatatccga ttctttggca tacttttcca ccattgcacg
     5221 gtgtttcaat acttcttctg agacactagt tccgccagga gaagtgccag aactaccatt
     5281 tccctcactg tcttcaccaa acaacaaacc cacacacatc aaaataccca taaaagagat
     5341 ggtcagaaaa gttaatgaaa taacacccac tttcttcaag ttcatttttt ctcacctgac
     5401 cttgattttg gacgatggtt cattcttttt tgtacttttt gattggtctg ctttggcaga
     5461 gtggacctct gtttcacttg ttggaaagaa tttttcttac ttgctgattg gcttttccct
     5521 tttgaaatga tttcttgact agttagtttt cgtttgattt cgggattcag atttgctctc
     5581 acaatccgct ttggtgcttc ttttttcagc atcatatctt tctgaggttg agctgcttta
     5641 tctattggaa tattcgccct tctttttccc tgacgatttg ttgcttcact cagtatcttg
     5701 cgtttttcgt ccattttcgc atttcttcgt tgtcgttgtt tttctcgcaa atccactcga
     5761 tccgcttttt cttggaacag tccgcgcttg aattcttctg gtgctttttt cgacttttcg
     5821 attcctttat ggagattgta tttcaaattc gttggcgtat tccttacctg atcctttact
     5881 tgtttagtct tatctttcac caaagcttgg gtatccaaaa ctttgccgat ttttctccca
     5941 gccaaattca ttcgggattg tttcttatta tttgaaactg gattctcctt agttaagtca
     6001 gatagtattt cgtgatcgtt cggtaatcgt gaattctttc gtagagaaga accggatggc
     6061 ttaaatttgg attttgattg ttcctttgcc aacttatttc cgactgctgc accaccacca
     6121 aaagccaaag ctcgtcccac atttcgattg agttgccgca attttcgatt cattaacatt
     6181 tgcggttttc gcatgacgcg tcttcccatt gactggctat cattactctg gaggttgaac
     6241 atactcataa tgtcgcccaa tttgaaatag ataccggcaa aggtcacaat ttgcagaaaa
     6301 gcaaccatga aaaatggata ggttgccgaa atattgaaaa acatcgtgga gatgctaaat
     6361 gcagtcgtaa tcaccaaagt aacacccgct cgcatcataa tcgtattgaa taaacgaata
     6421 atcgccttct ttcctaaact ttcataagtc ggcaacatgg acaataagaa actaattggc
     6481 agaaacatcg caaagataat gaagagaatt tgcgagaaca acataatccc tgttagaaga
     6541 aaaacaaaga aggaaatgcc aatattgaac aataccaaaa agaccacggt ccctaaacgg
     6601 ttcatggtct tggtaatact gagattcgca ttgtcattgt cttcaatctc tgccttgacc
     6661 gcttcttccc gatctttgcc tttgttttca tctggactca cagatagaat cttgttgacc
     6721 cggtcttcac caatttcttc tatgttggaa tcatcaaatt gcagtagtaa ccaaggttgt
     6781 tggacttgaa tcgaaaacaa actatcccga atcaaatcca cactatcttt tccttggctt
     6841 tctgaattgg gtacaacgat ctcggttcca agggtaaggg ccgcttcact aacatctgag
     6901 ctaaagtcat tgatttttgt gatataggta ggcgcataag caatgaacga acccgatagt
     6961 agaaaaatca tcacaaaatt aatcacggct cgaactgctt tcgtggtttc tcgttttaat
     7021 aatccggtat acgccacata cacccccaga gctaaaatca tcaataatag aaacccaaaa
     7081 taaaatccgg aggtctggag tccattttca gtaataccag ccaatgtttg gatattcttt
     7141 ccaatacttt cagccgtatc cgaaatgaaa tctaatttgt aagcctcttg aaccacataa
     7201 cccgtggcgt tcgacaaata caaactcacc gtccaaatga aattagtaat ggcataaaga
     7261 ccatacatca cgcttttccc taagccatca ccccaattcc aaggcaacca atcccaagag
     7321 ctatccacaa agaaatctag ttgatagttg ttcagtgaat atttggaata tagatttcct
     7381 gcttcaactg tatcatcaac tagccctgtc gcctcggcta ccgtaccaac taaactcaat
     7441 agaactatca gagtgagtat gctaataaaa acgattccca caatccgaaa aagtttcttt
     7501 ttcatcccct cactccattc tctcttgaac aggtggacgg gtatcaaagg catggaagag
     7561 ttcttcaaag actggatgta cctgaaccac gcctacccga ccatacaaat cctgcatcaa
     7621 gcattggcca ttttccaaat ctcgtaggcg tttttgattg ctttcatctt ctcgatccaa
     7681 gccaaagaat tccaaggttt tcttgatttc atgaatatcg gtactgcgaa acgcaaactt
     7741 caacccgata ttattcttca agcgttcatc cagtaaatca tcactatttt gcgtgacaaa
     7801 gtaaaccccg gcattcatag cccgcccagc acgaacgagc ttattcgata atgttttccc
     7861 ttgcgctact tgaagaaaac tccacgcctc atctaagtct acaattttga agatttctcg
     7921 attactatgg ataaagtcca aggcaaaagt ggaaatgacg attagcatgg aaacactaag
     7981 caattccatg gtggtatatt cttcaaaagt ggtttctgca tctggtaaaa ccaaatccgc
     8041 tacttgaatg atatttaact gcctatctaa actaatcgac tgggtaatcg aaccatctga
     8101 gaaaagcaaa tgggcaaaat cataatcggt aaaggactcg atgtgatccg cgatattttc
     8161 cgcaactggt gagccttctt ttcgaagctc attaattact tgtaataatc cccgttgatt
     8221 gctttgggta accgtccgaa tggccttacg caacactgga aacttctccc catctcgact
     8281 agaaattcct gttaaaaagg tcagaatatc aattgctaga ctttcagaat ccttcttttt
     8341 ctccataatc acataaggat caagtaatcc ttgatttttt ggcttactag ttaagttgag
     8401 aatattgatt tcatccgcaa tttctggcaa ggtttctttc cagccacccc gttccccttt
     8461 tggatcgaca atcacagctt gtccaccaaa aaggacggcg taataaacta agagattgtt
     8521 actaaaggac tttccgccac cgagtgaacc gaggaatgcc gctgctagcg cattggtaac
     8581 tgaacccttg accccttgtg aggccaatgc cggtttcagg taaacattgc gccccgtatc
     8641 tacgttataa ccaaagtaaa tgccttccaa ctcccctaac atctgagtgg cgccaaaacc
     8701 aagtccggcg agaaaatcag acgtaacata ctggatataa tcattcatgt atcgcttact
     8761 agaaggtaag aattcttcat gtagccccat catatcgcca aaaggccgga ctagttttac
     8821 attggtatca tcataaaaat ctagcacttc atcacaccgg cgctttagtt catctactga
     8881 ttcggcactt actcgaacca catagctcag tttgtacatg gattctttgg actgatccaa
     8941 ggtagtttcc aattcatcta cggaatccaa tgcatctaag acattggaat ttgtttcatt
     9001 gtcagattga taggcatggt tgtccaagtc ttttaattcc tttttcttat tgcgaacggt
     9061 agcaagtgcc tttttgttcg tcacaatctc cacattcata ctagtgtcaa ttgggaatgt
     9121 aaattgttgt tgctggtaat agaaaagttc tgaggaggga aactccattt cccccacaat
     9181 tgtattaatt gttaagtacg ctacatatga ttcatgattc tcatgttcca tgcataagta
     9241 ccggggcttt tcttcaatca aacaacgact gggacgtaat aagtcgtaac gtttcaccaa
     9301 agtttccgac ttcagctttt tctttggtag ctgaaattca tattcttcaa atggtgtacc
     9361 cttttcgcca tagatatgtt caatcaaata catcagatca ctcggtgtga ctcgtctgac
     9421 tttaaatctt cgagccaatt tactttccat tagtttttca agcttggtat atcggcgaat
     9481 ttcttcattg gataaggaaa caaaatcgcc catcaaatga tgattcactc catagacaaa
     9541 ttcttgaaag ccggagaaga aagatttctt gagattcttt agattgacct cttcatctgt
     9601 cgcaatcagt ttgaagccaa taaaaaagcg ataatcaatc tgactatcgc caatcatgga
     9661 tactaatgcc tctgtctgta aatcaattcg ttgtttcgct acttctgtta atcgcccggt
     9721 aatttccttt tttgatcgtt cttgcgtggc acgaatacta ctttctgtgg caatttgtaa
     9781 ggcatgaatc ttgccttcac gattttgcgc aatcaattga cgaaagttat cgtggacttg
     9841 atatttctgt tcaggtgaca aaaaggagta attgtaaggt attaactcat agtaggcaaa
     9901 acattctccc tcttgattaa agacaagatt attttcaata tacttaatcg ggtacatagt
     9961 tcacactcct taccagagta atgggttcgt cccatcgttt cttctgttct ttgactttct
    10021 ttcctgcgaa agtgacctta aatcgtaagt gatagctaat aaaagatcgt aaaaatccca
    10081 atggtttctt accgtcaaac gttttttgcg acatgaacca agtaactgcc accggtatac
    10141 ctaagtattt taaaaaggcc ccctctatca tagatagtgg aggcacatgg gcaaatacga
    10201 tgacaaacag caaggataaa acaaaccaag ccatctggtt aaaagtcaca ggaaaaggca
    10261 gttgaaagtc attaattgca taaatgacct tttcaactgc ccaaatactg gtgtagcttt
    10321 ttattttctt catcaaatca ttcctttaaa aagaaggata gtttagaaat caactaccct
    10381 tcaataattt tgttaatatg gatactcaaa aacacctcga ctcgttacca aataattgcc
    10441 tccaatctcc aagtctctac cacacgcatc aaaatcaata tacgtcttca agtgttctgg
    10501 tacttctcca aaatcaccag cttctacaat caagaaatat gctacatcac tcatatcatc
    10561 gcaatctgga taatagataa tatcatccac atgctctacc atctcctcaa aagaattgaa
    10621 gaaagcatgt tgaatctcag aagcaacttc accaactggc gttccttcaa gttcctccgc
    10681 taagccacac aaccgattaa tttcctcaat tggcgtatac tcatcaatct caaagggaag
    10741 ttcataatca tgaattgcat attcctcgta ttcgtcattt aacccaattc gttctttcac
    10801 ttcatcaaaa tcgacaggtg gggtaaacca cgcccccacc aattcacctt cgttatattt
    10861 cccgaggttc gcaatataaa cacgcatctg ttccatcgtg cttccccctt acgcaccgac
    10921 aattcggtta aacaaactga gtaagacatc cttcacccca gaagcattaa agaccaatcc
    10981 gaccgcaatc aaagcaatca ccaaaaagcc aatcagtttg gaaaactccc gcttaaatcc
    11041 taaaaacaaa ccaatgacca caattgccag taagactaag gattgggcat tggttaaaaa
    11101 ccatt
//
LOCUS       DAZKCY010000082        10913 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000082 DAZKCY010000000
VERSION     DAZKCY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.083"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..804)
                     /locus_tag="ACG8XC_001980"
     CDS             complement(<1..804)
                     /locus_tag="ACG8XC_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9191666.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRI"
     gene            950..1753
                     /locus_tag="ACG8XC_001981"
     CDS             950..1753
                     /locus_tag="ACG8XC_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFP9191667.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1931..2878
                     /locus_tag="ACG8XC_001982"
     CDS             1931..2878
                     /locus_tag="ACG8XC_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9191668.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2946..3239)
                     /locus_tag="ACG8XC_001983"
     CDS             complement(2946..3239)
                     /locus_tag="ACG8XC_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFP9191669.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3267..3932)
                     /locus_tag="ACG8XC_001984"
     CDS             complement(3267..3932)
                     /locus_tag="ACG8XC_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFP9191670.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4072..4704)
                     /locus_tag="ACG8XC_001985"
     CDS             complement(4072..4704)
                     /locus_tag="ACG8XC_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9191671.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4711..5778)
                     /gene="liaS"
                     /locus_tag="ACG8XC_001986"
     CDS             complement(4711..5778)
                     /gene="liaS"
                     /locus_tag="ACG8XC_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFP9191672.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(5775..6506)
                     /gene="liaF"
                     /locus_tag="ACG8XC_001987"
     CDS             complement(5775..6506)
                     /gene="liaF"
                     /locus_tag="ACG8XC_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFP9191673.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDIEVIRI"
     gene            complement(6674..7153)
                     /gene="greA"
                     /locus_tag="ACG8XC_001988"
     CDS             complement(6674..7153)
                     /gene="greA"
                     /locus_tag="ACG8XC_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP9191674.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7289..8464)
                     /gene="mltG"
                     /locus_tag="ACG8XC_001989"
     CDS             complement(7289..8464)
                     /gene="mltG"
                     /locus_tag="ACG8XC_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFP9191675.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            8629..9789
                     /locus_tag="ACG8XC_001990"
     CDS             8629..9789
                     /locus_tag="ACG8XC_001990"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9191676.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(9829..10776)
                     /locus_tag="ACG8XC_001991"
     CDS             complement(9829..10776)
                     /locus_tag="ACG8XC_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9191677.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
BASE COUNT         3303 a         2253 c         1726 g         3631 t
ORIGIN      
        1 tatacgagga tatatctttt caatctcgct tagtagctga tcttcaaaat caatatgtcc
       61 gtcgaaaata attatctttt cgctatactg ccaaatatgg acatgatgaa catcctttac
      121 cccatcgatt gcttcaattt cttgttccat tttttcatag tcgatagcag gtgcactttg
      181 tatcaaaata gcggcagctt ttttgatcac cattatcgct tctatcaata tatatatcga
      241 aatgatcagt gtaataactg gatcgatcca gtagatatcg aaaaaacgga tcaaaattgc
      301 tccgataatc acacttaccg aagacagtgt atcacttaac aaatgcaaat aactagattt
      361 tatattcaaa ctttcatctg cttcttgtcg taataataat actgagaaca aattcgcgag
      421 caatccaata actgctactg ttagcatcaa atgactattt atcttttctg gtgaagtgaa
      481 ccgcttgaat gcttcaacgg ttaaaaacaa ggataatatg atcaatatga atgagttgat
      541 gaaagcactc aatatttcta aacgctggta gccatacgta cgttttttat ttgcttcttt
      601 tcgggccatt ttttgtgccc cgtaagaaaa aataattgcc aatgtatcac taaggttatg
      661 gaaagcatct gaaacaagag acaaactccc tgacataatc cctccaataa attctgataa
      721 agtgatcacc gcattcaaaa ccactactcc aaatagtttt tttgatgatc tctgtggctg
      781 tgtaggcact attccttcct ccattttatt tatattggat agtctttaga caagaataac
      841 atttttttga tatttcacca atcattcagc tgtttcctta aagcacttgt atttcacttg
      901 atacttcgct agaattgcaa gtgatgacac tactagatga ggagtactta tgacaacaaa
      961 acaattaatt atcagttcct ttatacgatt caaagaccta aaggaggcga gaaatgtccc
     1021 attttggaaa gccattgtct atcttttagt tttgagtata attatggctt tgccaatctc
     1081 ttttcaagtt tttcaagtct taaacaacat tcaattggat gggcaaaaga ttgctactcg
     1141 cattcctgat tttacaatcc aaaacgggca gcttcaaacc gaagaaaaag aaggattcat
     1201 ttaccaaaca aactccatta tcttcacatt tgatccagag ggaaaacgaa cagaacagga
     1261 catctctact gatttgatgg ggaattttgt tagtgtcggg atgttaaaaa acaaacttat
     1321 catagctctt cctaatacag gcacgacttc tgctttgttg aacaataacc aattagaact
     1381 tccctacacc aatgaatctt taaaaaactt aacaggtaag cagctccgtt cttttttaag
     1441 tgaagcatct ataccacttt ggataaaagt cctgacattt atattttcta tttatcctag
     1501 ttttctgaat ctgctgatca ctttgctatt taccaacatt gctgcttttc tctatgcacg
     1561 ctttcgacta acaaaagcta cttttcttga ttgtttaaaa acattgatct attcaatttc
     1621 attgcctact ataattgcaa caatcttgat ggtctttctt ccttcttttg acactagcgc
     1681 cttcattgct tttgcaggaa tctttatttt tgctcaagct gtcaaaggat gggcaaaaat
     1741 atcgatcaat taagaaataa agacaactcg ttatttctac ttcaatagac ccttttagag
     1801 gttgtgacat ttttttgtca cagccccttt tttatttgaa aaatatttct ttctcaaaaa
     1861 acaagcaagg cgcttcaaag ttcttcttgt atgcgctaca cttagagtaa agaaaggcgg
     1921 gaaattaact atgtcagaaa gaatcattta tgcagatcgt ccatttcgtg atgaatttat
     1981 cgaagaaatc catactatag cgcctggtta tatctttaaa acagaattac aaccagagga
     2041 tctacaaaat gtagagatca gtttgggctg gaactcagac taccaagaaa aactattagc
     2101 ctcttctacc ttgaaatggg tccaatctat ctcagctggt gttgatcagc taccccttga
     2161 gactttttca aagaaacata ttttactttc aaatggtagt ggcatccaca gtgaatcgat
     2221 tacggagcat gtcttaggaa tcatccttgg gtattctcgt ggtctttttc aagcacaaaa
     2281 ggcacagagt gacatgaaat ggttaggttc atcggtccat taccaatcta tccaagggaa
     2341 aaaaatacta atcattggaa ctggccatat tggaaaaatg attgcaaaaa aatgcaaagc
     2401 attagacatg aactgctatg gaatcaatac atccggtcat ccagcagaag gaatggaaaa
     2461 gactttttct ttaaatcaac taaaggaagt tcttcctgaa gtagatttcg tagtcaatat
     2521 cttacctcta acggaggaaa caactgggct ctttgatcag catatgtttg aacactttga
     2581 tccaaaatct gtattcatca atgttggccg tggtgcttct gttaagacac aagatctggt
     2641 gcaggcttta aataaccagc aactttcctt tgcagcatta gacgtttttg aggaagagcc
     2701 tcttccacaa gatcatcctc tttggaaaat ggacaatgta ctgattactt cccacattgc
     2761 aggtttgaca cctgattttc aaaagaaatt aatggctatt tttttaacca atctgaaaag
     2821 ctactttgcc acccatgagc ttcaaaccaa tcaagtaaaa ttgtctgcag gatattaata
     2881 gctgaaaaca atattttcaa aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc
     2941 tttttttatt gttttttaaa gtgataggtt ggctcctcca ggttgggatc ataatcaaca
     3001 agtaaatcat atcctttaaa gaaccattca tctgcttcct cgataaaaaa aagtgtccca
     3061 tccatttcca ctttctttac aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa
     3121 ccatcatgaa cctctgtttt gccgtagact tttccaaaaa aacgtattcc gtaagtttct
     3181 ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg gcgttaccgt taatttcatc
     3241 ccaacaacct actttctctt ttctttctat tgattcatct tatcgtctag aatatccaca
     3301 tcgcctgtct ctccaataac cagtagatta tcattttcac gaacatattc atctgctgca
     3361 ggagaagcga taaccgtttg atctactctt cgtatggcga ctactgttaa gtcaaaacgt
     3421 tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt taggattgga gactcggatt
     3481 tctgctaaag aatatttatc tgacagctct aagtagtcta gaatattctt tgatactaag
     3541 ttatgtgcaa tccgtatccc catgtctcgc tcagggtgta cgacacgatc tgctccgatc
     3601 ttctctagta cacgggcatg gtactcattt tgggcttttg ctaaaatatt cggtacgccc
     3661 atttccttta ccatcagagt aactaagatg ctggcttgga tatcttctcc aatagcgaca
     3721 attacatgat cgaaatttct tatacctaat gaacgaagtg tcatctcatc ctgtgcattt
     3781 gccacaacag catgggtggc tatattcata tattcattga ctcgatcttc attactgtca
     3841 atagccagta cttcttgtcc agcttcgatc agtgtacggc agatgcttcc gccaaatcgt
     3901 ccaaggccta caattgcaaa attttgtttc accattttcc tcctttagta tcttcttttc
     3961 tcagtataac aaagcctttc caaaaacaaa agcaagcatc attattgtaa aaaagccaag
     4021 aaaaataata gttattttct cagcttttga ttatcctctt tatcatcgtt tttatttcac
     4081 caaaccatgt ttaaacgcat agatagctgc ttgagttcgg tcttctacct ctaacttagc
     4141 taatatattc gacacatgcg ttttgactgt tttcagtgtg ataaacaatt catccgcgat
     4201 ttcctgatta cttttccctt cagaaatcag catcaaaatt tcattttctc gattcgttaa
     4261 ttcttcatgc aatactggat catttcgccg actcattttt tccatcattt ttgttgtcac
     4321 ttcaggttct agtactcgct cgccgcgttg cgtagccctt atagcatcag ctatttcatg
     4381 tgctgtagac gtcttcaaca aatatcctgc tgctcctgct tcaattgcag gataaacttt
     4441 ttcatcatca ataaaacttg tgacgataat gattttggct tttggccaat cttttaagat
     4501 tgcttttgtt gattcgatgc catccatttc ttccatgact agatccatta aaatgacatc
     4561 cggtcgaagt gccatcgcct tttcatagcc ttgacgtcca ttttcagctt ctccaatcac
     4621 ttcaatgtct tcttggatag ataaataaga agagacgcct aagcgtacca tttcatggtc
     4681 atctactaat aaaactttta tcatctttat ctatgcctcc ttcatcaaag ggaccttggt
     4741 ctccacgctc gtaccttgcc ctttaaaact aatgattttt actgttccac caataccagc
     4801 tactcgttct ttgatattgt tcaatccata acttcccgtt ttggtttcat tcatattaaa
     4861 tcctgtcccg tcgtcaatga tacgtaataa aagattgtta tctattttat gcaaatagac
     4921 ttctaattca ttagccttag catgtcttaa tgtgtttgat aacaattctt gaacgattcg
     4981 gaacaagtga tcctcaatgg aactagttaa ttttacatct tctacctccc attttagcga
     5041 aatctggatt ttattctgca attccttcaa taactgctca atgccttgtt ttaaactttt
     5101 ttcttctaaa ttgactggac gcagatgaag aagcagcgca cgcatctcag actgggaagc
     5161 gttgatgatc tctgccacca ttttcaactg cttacggaac atttctggtg tctcactttt
     5221 ttctgcttgc tccgtcaatg ccgacatcat catcatcgct gcaaataatt gctgtgaaac
     5281 tgaatcatga agctcacgag ctaatcgatg cctttctaat tttaaaatct cttctttggt
     5341 ttgtccatct acatattgag gacggctagt cataatctgt aattcactgg atacttcgat
     5401 cattttttct tttattttag taatttccat atcaatatct ttgatgtaca gatcatccga
     5461 cgcgcttggg atcggctgat gcaaaagttt ggattcataa ttgccagaag aaagtgcacg
     5521 aagcttttct tcgattttcc cgtattttgc cttttgaata agtgataaca atagaaatac
     5581 gatcaatcct acacctaatg aaattgccat caaatggaag ataagcggaa cgtataataa
     5641 acgcgcacgc agcaattctc cccaccaatg actttggtta ctggcataaa aataagtgaa
     5701 aagtgaaaat agaataataa ggaaagccgc aatccccgaa taaacagcta gcattgcttt
     5761 ggatattctt cccatcatat tcgtatcacc tcgatatctc caaccaaagt attggtaatg
     5821 atcttcaatc tgcgaggatt ttcatcataa tcactgctgc agattttgat ctgttcgttt
     5881 tttaaggaaa actgctcttc ttcaaacaag acatttccta ctaatgtcgc atgctctaaa
     5941 cgaatagcca ctcccagggg tactaagatc cgtgttctgc ctatcccttt tcttacaatc
     6001 acaatattat catcttttgg caaaagtgta ttgcccaaat cgatgatagt atcaccagaa
     6061 atgatggcaa tgttgatatc atcccattca tagacgtcgt ttccaatacg ttggttacca
     6121 aataattgct gtcttttacg ctcattatta tgtgttttta attgctctgt ttggaccatc
     6181 atgatctgtt tttttcgcca aaaagcattt tttgtcaaat ctattcctga aatctctaca
     6241 cctttcagcc cgataaataa aacggccaat acaaccatca tccataaagc aggactgttg
     6301 actaagctaa aaaaaatgac caagctccct aagatcaatt gaaaattttg aaatttagta
     6361 cgaggatatt tgcgcatagc tagatatata ttgaaaatac cgaatattac taaaagcaaa
     6421 agtccagtat tgtttacgat ttgccaaaca gcaaataaca gcagcagtgc ttcgactacc
     6481 acaaaaaaac gccaagaact attcatcgct ttttctccct cctgacacat tctcatttca
     6541 tcatacctat ttttttgact gttggctata ggactttaga tgtaagatcg ttcagtgaaa
     6601 cctgcaatac tcaaagagaa aagatctaga gtgcgtcatc agtaagatga actctctaga
     6661 tctttcagtc agactacgct tgttctactt tgacgatttt aactaacata tcgccacctg
     6721 gtgtagcaat ggttacttca tcatttttac gtttgccaat caatgcttgc gcgataggag
     6781 aatcattgga aatctttcca gaaaacgggt ctgcttcagc acttccgaca atcgtgtact
     6841 cttcttcatc tccatcaggc aactcgatga acgtaactgt tttaccgatc gatacctcat
     6901 ctgcatctac gccgtcatta tcgatgattt gtgcaaaacg gatcatattt tcaattgttg
     6961 tgattcttcc ttcaacaaat gcttgttcat ctttagctga ttcgtattcg gagttttcgg
     7021 aaagatctcc gaaaccgcga gcgattttga ttcgttcaat gatttctccg cgtttaactg
     7081 tttttagttc ttctaattct ttttctaatt tttctttacc ttctagagtc attggataaa
     7141 ctttctctac catgtgtaac gtctcctttt ttcgataaaa taccgcaaaa ggtaatcttt
     7201 tgaggaatat agtccaaaaa agattaccac gcttttctta aaatgtaaat gtttttctaa
     7261 aaatgatcaa tgactagccc ctgtttttct aagaattatt tacatatttt tgcactagtt
     7321 ccatatgttc ttcatacgtt ttagcgaaat aaacatttcc agtgctcgta tctgctacga
     7381 aataataata gtcattttct gctggttcta atactgcctt gattgcctct tcgcttggat
     7441 tgtcaaaagg accaggccca tagccggtat tggtatacaa attgtatggt gaatctacag
     7501 ctgtatcttc atacgtcacc aattctttat gttctcctaa tgcatataaa acagagatat
     7561 ctgactgaag tggcatattt gccttgattc ggttaaagaa gacttgtgca atatttttac
     7621 gatcattttc ttttacgcct tctttttcaa ctagagaagc caaagttaaa acttcttgta
     7681 cagtcaaatt tttctgtttg atagctgaat aatagttttg catgaccgta tttgttttgt
     7741 tcaccatctc aatcacaaga tccttaagcg aagtactcgt gtagtaatca taagtagctg
     7801 ggaataagta gccttccaaa cggtattttg tgttctcagc tttagaggca ctttccaaca
     7861 attctgggaa tttttgatac aactcgttaa agaaagtctc atcatttact aaatctaaaa
     7921 attctttctt gtctttacca gttaccttcg ctacccgctc agcaatctga tcgatgtcgt
     7981 agccttctgg aatggcaatt ttcccatctg caacttttgt tggctcagat gtgccgcctt
     8041 cttgcaactg cttgccaatc tcatcaagag tcatattagg cgctaactga taataaccag
     8101 cttgaaagcc agttaagttt ttaaattttg tataataatt gaaaacaatc ccacttttaa
     8161 taatattgtc tttctccaaa atttcgccaa tctgcttatt agaagagccg cttgggattt
     8221 ccacttggac taattggtcg tccgatttat ctaaaggctt caacccgcta tctacataac
     8281 gatatacggt aaacccgaga aatccgccaa taatcaataa tgtaagagcg atcactaaaa
     8341 tgatcttcct cacgatctta tcttcttttt tacgcagatt tctccgcttg tcgattatat
     8401 ctgctgtagc tgccttactg gcttcttttt tgtctttgct tgggtgttgt ttgtttcctg
     8461 acaaaacaac tcctccttac tatcatcttc gtaactctgt ttattataca tgaaatttct
     8521 actgccgtga attattttac caaatattat gtcaaaattg taagtggcaa atgctctttt
     8581 tgaggattac aatgaaaaat agttaaaaat ggataggaag gatttaatat ggcaaaagga
     8641 aaacatcaaa aaaaacagca gatctctctt tcttttctcg cagttttgtt gctggctgtc
     8701 agcttctttt caacaggcat cattacagga tggcaattaa caaaacaacc tcaaccacaa
     8761 atttataaaa cggaacccgc acaacttgct tccaatttaa catctaatga gcagatgctt
     8821 gccagcaaaa ttgatcaatt actgcaaaaa tcagattata tcggttcgat ttatgtacgc
     8881 aaaaataatc gagtgatatt agaaaaagga tatgggtacg caaatctacg cataaaagaa
     8941 tcgaattctc cttctcttta ctatcaaatc ggttcgatcc aaaaagcgat gactgcttta
     9001 cttatcctaa aacaaattca atctggcaag ataacttttg acacaaagct atctgaattt
     9061 tatcctcaga ttcctggaag tcagcaaatc tcaatcaaaa atatgcttta catgcgctct
     9121 ggtcttcatc gaaccgcttc tccaaaaaaa cctatgtcag atagtgagat cattcagttt
     9181 gcccttcatc atttgaaatt tactggttat gatacgtatc attatgaacc attaaattat
     9241 acattgctta caggggtatt gatcaaacta atgaatcagc cttatgaaac tcttttaaaa
     9301 aaagaaatca ttcggccact ccatctagaa catacagatt tttatgaaaa tgtaaaaaat
     9361 tcccctcagc atgctgtatc ttaccaaatg tctgcaactg acgattattc aaaagcattg
     9421 gtcgagcctg aaacagacat ccgaaatgaa ttaggcacag gaaatataag tatgtctgtt
     9481 tatgacttga acaaattctt taccagtgtt ctgtctggcg atattatacc aaaagaacta
     9541 cttttctcat tgtggcaaaa taatggtgat aaacatcctt acagcggcgg tgtttacagt
     9601 ggaaatgatt atatccttgc tcagggaaat atcaatcgat tccatgcagt agcggcgatg
     9661 aaaaaagaca caaaagatgc aatcgtcatg gaaagtaata tccaatctga taaaaacatc
     9721 aaattgccgg ccactgattt aagaaatcag atttacgaac tgatggaagg tgttaattta
     9781 aaaagctaag tcgagacaaa aaaaccagca ggatgctggt tttttgtttc aatcgatttc
     9841 tgtctttaaa taacgttcca ttccgttcaa tatatcaaat ttgggttgat aacctaaaga
     9901 acggatcttt gaaatatccg ctaaagaatc acgaatatcc cctgaacgct ccgcttcata
     9961 tttaagcgct agctctgttc ctaagatttg atcaattgaa tgaatcaatt caagtagcgt
    10021 agtgcttttt cctgtaccga cattgaattg ctgtcctaaa gctttttctt ccttagcaac
    10081 aagtaaaagt gcttgtataa catcttctat ataaacgaag tctctagatt ggctaccatc
    10141 accaaataaa gtaaattctg ttttttctcc tgccaattgt tttttgtatc ggtccactag
    10201 aatcgaaata actccgctat atggagagtt aggattttga tttggaccat aaacgttaaa
    10261 aaatcggaca gcactagtcg gaacatcata taaatgacag taatccaaaa catattgttc
    10321 tgctgcaaac ttatcgatgg catatggtgt taaaggacgg atgaccgatt cttctttttt
    10381 tggcaacgtt ggttcatcgc cataaactgc agctgatgaa ctaaatacca atcgttttaa
    10441 accagattga tattttcgta ccaattcaag caataaaagc gcactgtcaa aatttacacg
    10501 atgagtttct agaggacgtt ctacagaatc tgccacacta gctacagcag ctagatggaa
    10561 aatataatcg aaatggtatt ctttcatgac cttttccatc aaatttttgt cagcgacatc
    10621 cccttcaata aacgtgatgt gcttcgattc atccagattc tcttttttcc ccatcgataa
    10681 atcatcgatc acaactacat tgttttcttt tcctagataa ttcgcgagcg tcgagccaat
    10741 gaagccggcg ccaccggtaa tcaagtaatt caacattttt tctcctcttt tcaagataaa
    10801 atttattact aaaaatagct agaattattt ttgctattaa ctcttcctaa aaatagccaa
    10861 aaaacaaacg ttcctacagt gttttcatag actaattcat tgttaagagc tgc
//
LOCUS       DAZKCY010000083        10619 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000083 DAZKCY010000000
VERSION     DAZKCY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.084"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACG8XC_001992"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACG8XC_001992"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9191678.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="ACG8XC_001993"
     CDS             2714..2938
                     /locus_tag="ACG8XC_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191679.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            3055..3552
                     /locus_tag="ACG8XC_001994"
     CDS             3055..3552
                     /locus_tag="ACG8XC_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9191680.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            3641..4033
                     /locus_tag="ACG8XC_001995"
     CDS             3641..4033
                     /locus_tag="ACG8XC_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9191681.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            4017..6464
                     /locus_tag="ACG8XC_001996"
     CDS             4017..6464
                     /locus_tag="ACG8XC_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9191682.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            6467..8644
                     /locus_tag="ACG8XC_001997"
     CDS             6467..8644
                     /locus_tag="ACG8XC_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP9191683.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDILFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            8641..9642
                     /locus_tag="ACG8XC_001998"
     CDS             8641..9642
                     /locus_tag="ACG8XC_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFP9191684.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            9639..10574
                     /locus_tag="ACG8XC_001999"
     CDS             9639..10574
                     /locus_tag="ACG8XC_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9191685.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
BASE COUNT         3527 a         1944 c         2239 g         2909 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat aactggtttc tatcaaacgc
     2761 tcaatcactg gtgcttttag caatcgttgt gattggcttg tatcttggct tcaagcgtga
     2821 gtttagcaaa ctgattggct ttttaattat tgcgattatt gcggttggct tagtcttcaa
     2881 cgctgctgga gtaaaagaca ttttactaga gctattcaat cgcattattg gtgcttaaat
     2941 aaaaccgttc ttttgtggaa tataagtggt tttcttatgt tccgcaaagg aatggtacac
     3001 caaacgaagt gcggtaggga tttttgaatc tctacaaaga aaggacgtga atatatggac
     3061 gatatgcaag tctatattgc gaatttaggc aaatacaatg agggcgaatt ggtcggtgcg
     3121 tggtttacct ttcccattga ctttgaggaa gtcaaagaga aaatcggctt gaatgatgaa
     3181 tatgaggaat acgccattca tgactacgag ttacccttta cggttgacga atacacttcc
     3241 attggcgaac tcaatcgact atgggaaatg gtatcggaat tacccgaaga attacaatcg
     3301 gagctatctg ctctgctcac tcatttttca agcattgaag aactaagcga acatcaagag
     3361 gatattatca ttcattccga ttgtgatgat atgtatgacg tggcacgcta ctacattgaa
     3421 gaaacgggtg ctttaggcga agtaccagct agtcttcaaa actatattga ttatcaagcc
     3481 tatggtcggg atttagacct ttcaggaacg tttatctcaa ccaatcatgg gatttttgaa
     3541 atcgtctatt aaatctgtcg gtacattact actggcagat tttctatttt acggggtggc
     3601 tcaatcagct acccctattt tttatgaaag gattgattac atgaagaaaa tacgaagcta
     3661 taccagtatc tggtctgtgg aaaaggtact gtattctatc aatgatttta gacttccgtt
     3721 tcccataacc tttacgcaaa tgacatggtt tgtcgtgtca ctctttgcag tgatgatact
     3781 tggcaacttg ccccctcttt ccatgataga gggagcattt ctcaaatact ttgggattcc
     3841 tgtggctttc acatggttta tgtctacaaa aacttttgat ggtaaaaagc cttatggatt
     3901 tttgaagtct gtcattgctt atgcactgcg accaaagctg acctatgcag gaaaaaaagt
     3961 aacgcttggc agaaaccagc cacaagaagc cattacagca gttaggagtg aattttatgg
     4021 catatccaat taaatacatt gaaaacaatc tcgtctggaa taaagacggg gaatgttatg
     4081 cttactatga gcttgttcct tacaattact catttctaag tccagaacag aaaatacaag
     4141 tgcatgattc tttcagacag cttatcgcac aaaatcgtga tggcaaaatt catgctttac
     4201 aaatcagtac agaatccagc atacgttctg cacaagagcg ttccaaaaat gaagtcactg
     4261 gcaagctcaa agcggttgcc tatgacaaaa tcgaccaaca gacagacgct ttaatatcca
     4321 tgattggcga aaatcaagtg aactaccgtt tctttatcgg ctttaagttg cttctcaacg
     4381 atcaggagtt ttctatgaaa agtcttaccg ttgaagcaaa aaatgctttg tctgattttg
     4441 tctatgatgt gaaccataag ctgatgggcg attttgttag tatgagtaat gatgaaatcc
     4501 tgcgttttca gaagatggaa aagctcttag aaaataaaat ctctcgtcgt ttcaaaatcc
     4561 gcaggttaga taaggacgac ttcggctatc tgattgaaca cctttacgga cagacaggca
     4621 ctgcctatga agagtatgag taccatctat caaagaaaaa gctggataat gaaacgctga
     4681 ttaaatacta tgacttgatt aagcctactc gctgtttggt ggaagaaaaa cagcgatatt
     4741 tgaaaatcca gcaggaagat gaaaccgtct atgtagctta ctttaccatt aacagcattg
     4801 tcggagaact ggacttcccg tcctctgaaa tcttctacta ccagcaacag caatttacat
     4861 tcccgattga tacgtcaatg aatgtggaaa ttgtagcgaa tcgtaaagcc ctatctactg
     4921 tccgcaataa aaagaaagaa ctgaaagact tggataacca cgcttggcaa agtgataatg
     4981 aaaccagctc caatgtggcg gaagctctgg aaagtgtgaa tgagctggaa accaatttag
     5041 accaaagcaa ggaatctatg tacaagctgt cttatgtggt aagggtatca gcaaatgatc
     5101 ttgacgaact caaacgtcgt tgtaatgaag tgaaagattt ttatgacgat ttaagcgtaa
     5161 aactggtacg accatttggg gatatgctcg gcttacatga agaattttta cctgccagca
     5221 agcgttatat gaatgattat attcaatacg tgacctctga tttcctcgct ggtttaggtt
     5281 ttggtgctac tcaaatgctg ggggaaaatg aggggattta tgttggctac agcttagata
     5341 ctggacgcaa tgtctatctg aaacctgctc ttgccagtca aggggttaag ggttcagtaa
     5401 ccaatgcgtt agcgtcggct tttgttggtt cgctgggtgg tggtaaatcc tttgcgaata
     5461 accttatcgt ctattatgcg gtgctttatg gggcacaagc agtgattgta gacccaaaag
     5521 cagaacgtgg cagatggaaa gaaaccttgc cagagatttc ccatgaaatc aatatcgtca
     5581 ctctgacttc tgatgagaaa aacaaaggct tacttgaccc ttatgtgatt atgaaaaatc
     5641 ccaaagattc tgaatcactg gctattgata ttctgacatt ccttacgggg atttcctctc
     5701 gtgatgggga acgcttccca atccttagaa aagccattcg tgcagtaacc aatagtgaag
     5761 tacgagggtt gatgaaagtg attgaggaat tacgggttga gaatacgcca ctaagtacca
     5821 gtatagccga ccatatcgaa agttttacag actatgactt tgcacattta ttattcagta
     5881 atggttatgt ggagcagtct atcagcttag aaaaacaact gaacattata caggttgcgg
     5941 acttggtact tcccgacaag gaaacttcct ttgaggaata taccactatg gagcttttat
     6001 ccgttgctat gctgattgtc attagtacct ttgctttaga ctttatccat acagaccgaa
     6061 gcattttcaa gattgtagat ttagacgaag catggagctt tttacaggta gcacaaggaa
     6121 aaacactatc tatgaagctg gttcgggctg gtcgtgctat gaacgctggg gtatatttcg
     6181 tgacccaaaa tacagacgac ctcttagatg aaaaactgaa aaataacctc ggcttaaaat
     6241 ttgcatttcg ttccactgac cttaacgaga ttaaaaagac cttagccttt tttggtgtag
     6301 acccagagga cgaaaacaat cagaagcgat tgcgtgattt ggaaaacggg caatgcctta
     6361 tcagtgattt atatggtcgt gtcggtgtga tacagttcca ccctgtattt gaagaactgc
     6421 tccatgcctt tgataccaga ccacctgtgc gaaaagaggt gtaaatgtga aaccatcaat
     6481 agtaaacaga ataaaatcaa actggacgct gaaacgtcta ggtaaagtgg caatgacagt
     6541 ggctttcaca cttgtgattg ccatttttct tttagccatg ctgggaacgg tggttcaagc
     6601 tgcgggcttg gtagatgata cggtcaatgt ggcaaatgaa tacagccgat acccacttga
     6661 aaactatcaa ctggattttt atgtggataa tagctggggc tggcttccgt ggaactggtc
     6721 ggacgggatt ggaaaacagg tcatgtatgg actatatgcc attaccaatt ttatttggac
     6781 aatcagtttg tatgtttcca atgcgacagg ttacttagta caggaagcct attccttaga
     6841 cttcatttcc gctacagcag attccattgg taagaatatg cagaccttag ctggtgtgag
     6901 tgcaaacgga ttttcaacag agggtttcta tgttggattc ctcttactct tgattttggt
     6961 tcttggggtt tatgttgcct atacgggact gataaagaga gaaaccacaa aggcaattca
     7021 tgccattatg aattttgtgc tggtgtttat cctatcggct tcctttattg cctacgctcc
     7081 cgactacatt aaaaaaatca atgacttttc atcagacatc agtaatgcca gtttatcact
     7141 tggcacgaag attgtcatgc cccattccga tagtcaaggc aaggacagcg tggacttaat
     7201 cagagatatc ctgttttcca tacaggttca gcaaccgtgg ctactgcttc aatacaacag
     7261 ttcagacatt gaaagtatcg gtattgaccg tgtggaaagc ctgctctcca ccagcccaga
     7321 ttccaacaat ggcgaagaca gagaaaaaat tgttgcggaa gaaattgaag acagaagcaa
     7381 taccaatcta accattacaa agaccattaa ccgtttaggt acagtcttct tcctatttgt
     7441 cttcaatatt gggatttcca tatttgtatt cctattaaca ggaatcatga ttttctcgca
     7501 ggtacttttt atcatctatg ctatgtttct gcctgtgagc tttattttaa gcatgattcc
     7561 atcatttgat ggtatgtcaa aacgagccat aacaaagctc tttaatacca ttttgacacg
     7621 agctggaatc acattgatta ttacgacagc atttagtatt tcaaccatgc tctatacctt
     7681 atcggctggt tatccgttct ttttgattgc ttttctacag attgtgacct ttgcaggaat
     7741 ctacttcaag ctgggcgatt taatgagtat gttttctcta cagagtaacg attctcaaag
     7801 tgtgggaagt cgtgtgatga gaaaacctcg tatgcttatg cacgctcaca tgcaccgtct
     7861 acagcggaaa cttggacgtt ccatgactac tctaggggct gggtctgcca ttgttacagg
     7921 taaaaaagga cagtcgggtt cggggagttc tgcaaggaca caagcagatc actcccgacc
     7981 agacggaaag gaaaaatcaa cacttggaaa acgtatcggt caaaccatcg gtacagtagc
     8041 tgataccaaa gacagaatgg tagacactgc tagtggtttg aaagaacagg ttaaagattt
     8101 gccgaccaat gcaagatatg cagtatatca aggaaaatcc aaagtaaaag agaatgtccg
     8161 tgatttaacc agtagtattt ctcaaaccaa agcggacaga gccagtggac gcaaggaaca
     8221 gcaggaacaa aggcgaaaaa ccattgcgaa gcgtcgctct gaaatggaac aggtcaaaca
     8281 gaaaaaacag cctgcttctt ctgttcatga aagaccgact acaagacaag aacaatatca
     8341 tgatgaacag acctcaaaac agtctaatat tcagacttca tataaggaat ctcaacaagc
     8401 caaacaagag cgtccagcag ttaagtccga tttttcaagt ccaaaagtgg aacgccaagg
     8461 caataccgtt caagaaaaaa ccgttcaaaa gccagcaact tcaaccacta cagcagatag
     8521 aacttcacaa cgtccaatca caaaagaacg tccgtctact gttcaaagag taccactaca
     8581 aaatacaaga agtagaccac caatcaaaac cgccaccatt aagaaagtcg gtaagaaacc
     8641 atgaagttga aaactttagt gattggtggt tctggattat tcttgatggt cttctcactg
     8701 cttctgtttg ttgccatttt attttcagat gaacaggaca gcggaatttc caatattcat
     8761 tatggaggtg tgaatgtttc cgcagaagtg ctggctcata agcctatggt agaaaaatat
     8821 gccaaagaat atggcgttga agaatatgtc aacatacttc ttgcgattat acaggtggaa
     8881 tcgggcggta ctgcggaaga tgttatgcag tcctcggaat ccctcggtct tccacctaat
     8941 tcattgagta cagaagaatc cattaagcaa ggtgtgaagt atttcagtga attattagcc
     9001 agtagcgaaa ggctcagtgt agatttagaa tcggttatcc agtcctacaa ttatggtggt
     9061 ggtttcttag ggtatgtggc taatcgtgga aataaatata cctttgaact ggctcaaagt
     9121 ttctcaaaag agtattcagg tggcgaaaaa gtgtcttacc ccaatcccat agccatacct
     9181 atcaatgggg gctggcgata caactatggc aatatgtttt atgtgcaact ggtaacgcag
     9241 tatcttgtca caacagagtt tgatgatgat acggtacaag ccatcatgga cgaagcactg
     9301 aaatatgagg gctggcgata cgtttacggt ggagcttccc cgactacttc ttttgattgt
     9361 agcggactga cacaatggac gtatggaaaa gctggaatta acttaccacg aaccgcacaa
     9421 cagcaatatg atgtgaccca gcatatccca ctatcggaag cacaagctgg cgatttggtt
     9481 ttctttcatt ctacctataa cgctggctct tatattactc atgttgggat ataccttggc
     9541 aataaccgta tgtttcatgc aggcgaccca atcggttatg ccgacttaac aagcccctac
     9601 tggcaacagc atttagtggg agcaggacga atcaaacaat gagaaaggaa gatttaatga
     9661 tgaaatttag aaaaaatcag aataaagaaa aacagatacc aaaggaaaag aaacctcgtg
     9721 tctactataa ggtcaatcct cataaaaagg ttgtgattgc cttgtgggta cttttagggc
     9781 ttagtttcag ctttgcgata ttcaagcact ttacagctat agatactcat actattcacg
     9841 aaacaactat catagaaaag gaatacgttg atactcatca tgtagaaaat tttgtagaga
     9901 actttgcgaa agtctactat tcatgggagc aatccgataa gtccattgat aatcgaatgg
     9961 aaagtctaaa aggctatctg acagatgaac ttcaagctct caatgttgat acagtacgca
    10021 aagatattcc tgtatcgtct tctgtaagag gatttcagat atggacggta gagccaactg
    10081 gcgacaatga gtttaatgta acctacagtg tagaccagct cattacagag ggagaaaata
    10141 caaagaccgt ccactctgct tatatagtga gtgtctatgt agatggttct ggaaatatgg
    10201 tactggttaa gaatccgacc attaccaaca tacctaagaa atcaagttat aaaccaaaag
    10261 ccattgaaag tgaggggacg gttgattcca ttacaaccaa tgaaatcaat gagtttttaa
    10321 cgacgttctt caagctctat cctacagcga cagccagtga actttcctac tatgtgaatg
    10381 acgggatatt aaaaccaatc ggaaaagagt acatctttca agaactggta aatcctattc
    10441 acaatcgtaa ggataatcaa gtcacggtat cgctgacagt ggagtatatc gaccagcaga
    10501 ccaaagcaac gcaggtatct caatttgatt tggtacttga aaagaacggg agtaattgga
    10561 agattataga ataacaaata ttggtacatt attacagcta ttttgtaatc acgtactct
//
LOCUS       DAZKCY010000084        10323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000084 DAZKCY010000000
VERSION     DAZKCY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.085"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..415
                     /locus_tag="ACG8XC_002000"
     CDS             185..415
                     /locus_tag="ACG8XC_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFP9191686.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            512..1813
                     /gene="murA"
                     /locus_tag="ACG8XC_002001"
     CDS             512..1813
                     /gene="murA"
                     /locus_tag="ACG8XC_002001"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9191687.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1826..2002
                     /locus_tag="ACG8XC_002002"
     CDS             1826..2002
                     /locus_tag="ACG8XC_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9191688.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2137..2433
                     /gene="yidD"
                     /locus_tag="ACG8XC_002003"
     CDS             2137..2433
                     /gene="yidD"
                     /locus_tag="ACG8XC_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP9191689.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2618..3085
                     /locus_tag="ACG8XC_002004"
     CDS             2618..3085
                     /locus_tag="ACG8XC_002004"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9191690.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3170..4054
                     /locus_tag="ACG8XC_002005"
     CDS             3170..4054
                     /locus_tag="ACG8XC_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9191691.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4168..4404
                     /locus_tag="ACG8XC_002006"
     CDS             4168..4404
                     /locus_tag="ACG8XC_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFP9191692.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4492..4572
                     /locus_tag="ACG8XC_002007"
     tRNA            4492..4572
                     /locus_tag="ACG8XC_002007"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4526..4528,aa:Tyr,seq:gta)
     gene            4579..4650
                     /locus_tag="ACG8XC_002008"
     tRNA            4579..4650
                     /locus_tag="ACG8XC_002008"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4611..4613,aa:Gln,seq:ttg)
     gene            4775..4945
                     /locus_tag="ACG8XC_002009"
     CDS             4775..4945
                     /locus_tag="ACG8XC_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191693.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5165..5872
                     /locus_tag="ACG8XC_002010"
     CDS             5165..5872
                     /locus_tag="ACG8XC_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9191694.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5931..6575)
                     /locus_tag="ACG8XC_002011"
     CDS             complement(5931..6575)
                     /locus_tag="ACG8XC_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFP9191695.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6701..6988
                     /locus_tag="ACG8XC_002012"
     CDS             6701..6988
                     /locus_tag="ACG8XC_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191696.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7096..7416
                     /locus_tag="ACG8XC_002013"
     CDS             7096..7416
                     /locus_tag="ACG8XC_002013"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9191697.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7497..8810
                     /locus_tag="ACG8XC_002014"
     CDS             7497..8810
                     /locus_tag="ACG8XC_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP9191698.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8807..9526
                     /locus_tag="ACG8XC_002015"
     CDS             8807..9526
                     /locus_tag="ACG8XC_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFP9191699.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9654..10205
                     /gene="raiA"
                     /locus_tag="ACG8XC_002016"
     CDS             9654..10205
                     /gene="raiA"
                     /locus_tag="ACG8XC_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP9191700.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3436 a         1705 c         2203 g         2979 t
ORIGIN      
        1 ggaataaggg actgcgacaa gacttttgtc tcagtccctt tttttgcata tttttagcat
       61 ttatttccta aaaaatgtaa ggggattcct atatttttaa gtatacttgt ttttcctttt
      121 atacatttta tggtattatc attttattag ttattataaa acaatcgtta gaaaggacgt
      181 cattatgcaa gtatacggaa ttgatgcggt gcttcgaatc gtgagtcatt tggcgtttat
      241 ctacttagcc ttctggtctt tacgttcgtt acgcattgaa acttttttca gaccaatgca
      301 taatacgcaa attagaatgg caatcgtact tttttcaatt tttttagggt acactgcaag
      361 taattttttc ttagaagtta tcgctttatg ccgaaatctt ttcgttagcc tgtaaatttt
      421 aaaaaaaggt actattttaa aagcaaggat gttataatag aaaagatttt gagtaagtgt
      481 atgctcattg gaattttttg gagggaacaa catggaagag atcatcgtaa gaggtggcaa
      541 tcaattaaat ggaacagtaa gaattgaagg agctaaaaat gctgttttgc ctattttagc
      601 tgctagtttg ttagcagaag aaggtattac gacattagac aacgttccga ttctttctga
      661 tgtcttcact atgaatcaag tgattcgtca cttaaacgta gatgtagatt ttgatgaaca
      721 aaagaatcaa gtaacaatcg acgcttcaag acaattagaa atcgaagcgc cttatgagta
      781 tgttagtcaa atgcgtgctt caatcgttgt gatgggtccg ctacttgcca gaaatggaca
      841 tgcaaaagta gctatgcctg gtggatgtgc gattggtaaa cgaccaatcg atttgcattt
      901 aaaaggattt caagctttag gagcaaaaat tattcaaaaa aacggctata tcgaagcaat
      961 tgcggatgaa ttgatcggta acacgattta tttagatttc cctagtgtag gtgctactca
     1021 aaatatcatg atggctgccg taaaagcaaa aggaacaacc atcattgaaa acgtagctcg
     1081 cgaaccagaa atcgttgatt tagcaaacat tttgaataaa atgggcgctc aagtctatgg
     1141 agctggtact gaaacaatgc gtattgaagg cgtggatcat ttacatgcag tcaaccactc
     1201 aatcgtacaa gatcgtatcg aagcaggtac ctttatggtt gctgctgcaa tgacacaagg
     1261 caatgtgctg attgcagatg ctatctctga acataaccgc ccattgattt ctaaattgat
     1321 cgaaatgggt gcagaaatca ttgaagaaga aggcggagtc cgtgtcatcg gaccaaaaca
     1381 tatcttacct acagatgtaa aaacgatgcc tcatcctggt ttccctacag atatgcaagc
     1441 tcaaatgaca gctatccaat tagttgcaga aggtacaagt gtagtgactg aaacagtgtt
     1501 tgaaaatcgt ttccaacatt tagaagagat gcgccgtatg aacgctcatg tcaaaatcga
     1561 cggaaatgta gcaatcatgg atggaaacca tgaattgcaa ggtgcagaag tatatgcaac
     1621 agacttgcgg gcagcagcag cattagtttt agctggcttg aaagcaaatg gaatcacacg
     1681 tgtgcgtaac ttgaattatc tagaccgtgg atattataat ttccatatca aactacagca
     1741 attaggtgca gacgttgaac gtgtagacat ggatcaaact tctgctgaaa aaacagcaca
     1801 aacgattgct taaggaagtg gctgaatgag taaccaacga tatatcttat taactttgat
     1861 aaagatttta atggtgatct tattgcttat ccttcttttt gtggccggaa cgatgatcgg
     1921 atatggtgtc atcggtggcg gcaatccatt taaagtattt cagccaagtt tgtggattca
     1981 tattcgagac ttttttcatt gataaataag aatcacagtg gttctagtcg atatgaagtg
     2041 agccatgaag cagaacatga taaaagaaaa cagaagggaa cgttgacgtt agtcgcgatc
     2101 ccttttttta gtaaaagaaa agaaggaatc taattgatga agaagatatt gatttattta
     2161 gttagaggat atcagcgctt tatttcgcct gcatttccac ctagctgccg ttattaccca
     2221 acctgttcca attatatgat aaaagcaatt caaacccatg gcgcgctaaa agggacggta
     2281 atggggactg cacggatctt acgctgtcat ccttttgtca aaggcggtat cgattatgta
     2341 ccattgacat tccgattaac gcgaaataag aaagataaaa aagaaccaga ttataaaaag
     2401 gttgtgaaaa atacgggtag cactgagcga taaagaaaca atttggaaaa tagcttctta
     2461 tattttgcaa aattgtgagt tattgccgaa gtgttggatt ttgaacaccg tcaaaaaaaa
     2521 ggttgtgaaa aatacggcta gcactgagcg ataaagaaaa agaggaaatt tattgtcaaa
     2581 gtaacaactc cgcaactact tatatggggg acaaaaaatg atcgaagaac taaacaagaa
     2641 agatttacca tggtccttgt tattagatgc agatcctgat aaagaaaaag ttcaagtata
     2701 tgtttccaga gggagtggct ggatatggaa agaaaaagaa aaaacaatag gtgtactgat
     2761 ctatgtggca agggaaaaag aatacgaaat cgtcaatgta gcagtaaccc caacgtatca
     2821 aggaaagggg attggcggaa aattacttga gacagctttt cagaagcttt cacaactcgc
     2881 ttccagtcaa actcagatca tcatacgaac cggcagtact agcagtgcgg cgttacatct
     2941 ctatcaaaaa atgggctatg tggagattgg tcgagtcaaa gactatttta tccatcatta
     3001 ctctgagccc atttttgaaa atggagatca attaagggat caagtgattt tagcaattca
     3061 cttacctaaa aatagcttta actaataaaa tttcaattca agaaaatgct ttcaaatgct
     3121 aaatagatac attacaatag ctttagcaaa caaaaaggag aatattttta tgggaatatt
     3181 agtttttgat tttggtggat cggctgtcaa atatggttgt tgggatggca aagagattaa
     3241 aggtaaggga caatttgcaa cgcctgaatc ttgggaagaa atgaaagcgc aattatttca
     3301 agtttataaa aaaatactcg tttcttttga agtcgaagga attggaatca gctctcctgg
     3361 agtagtgaat aatgagaaac aggtgattga gggaatcagt gcgattcctt atattcatgg
     3421 atttaatatt ttccgagatt tggaaactct gtttcaactt ccagtaacga ttgaaaatga
     3481 cgccaactgt gccggaatgg ctgaatttta tgaaggtgct gcccgcgatt atcaagatgt
     3541 tgcttttgtt gtggcaggaa ctgggattgg cggggcgttg ttcacgaaag gacaaatcaa
     3601 taaaggggca catctttacg gtggagaatt tgggttgatg ttcttaaatg gcgataagac
     3661 attcagtaaa cttggtacag cggtacagat ggcttggcgt tattgtgaac gcaaaggtct
     3721 cgataaaaat gcttataccg gaaaagatgt ctttgaatta gcagaaactg gagatgcaat
     3781 agcaaaagaa gaagttgaga gtttttatac gtatcttaca aagggattat tcagtattca
     3841 attttcgttt gatccagaag tgatcgtatt aggcggcggg gtttctgcaa aagaagggct
     3901 cattgatgag atcaaaagcc gaatgaagaa gctgacagaa caattcgatc tgcatgattt
     3961 cgaaccgcag attttattat gtgaatacag aaacgatgct aatcttgtag gtgcagcagc
     4021 aaactatctc gctgtacgtc aaggggagaa ttaagagctt tagagaaggt gcagcttgca
     4081 taaaagaagt gaatgaaatg aataggagat ttttacctaa ggaatgtttt atttgctgca
     4141 gtatgataaa atatctgaga ggtgaaaatg atgtcaaaaa aaaataaaga tattgaagtg
     4201 cgtatagaag aagtgaaaaa agatatcaaa ggtaagaatt atgaagtcac tcagttattt
     4261 atagggaaaa aaatgattgg tgagatctta gcctatggac caaaagaata tgaaattttc
     4321 tttggtgaag aagattttgg aaaagaaaaa agtcttgaaa atgcgataga aacagtgatt
     4381 cgacactgga atctgcacga ataaaaaaat atcaaaaaaa ctttcaataa agggttgcaa
     4441 aaatctggaa aattaggtat tataactaag tcaggttgat acaaaccttt cggaggagta
     4501 gcgaagtggc taaacgcgac ggactgtaaa tccgttcctt agggttcagt ggttcgaatc
     4561 cactctcctc catttcattg gggtatagcc aagcggtaag gcaagggact ttgactccct
     4621 catgcgttgg ttcgaatcca gctaccccag tataccacaa tcgtaagctc tcgttagttc
     4681 gagagctctt ttttttgctt tgctaccttg ttccttgaag attctgctga tttaagatag
     4741 agttaagtta actacaagaa ttggagggat ccacatgaaa tggaaagaaa aagtagacga
     4801 agcagctgaa aagttatatg atttggtcaa atcagaaaaa tacaatattg aagtgaatat
     4861 tccgaaaaaa ggcggaaaag cggtccgggt aaaatcaaaa cgcccaacga atcatacaaa
     4921 aaaatggatg gctaaaaacc gctaataata acaaaagatc cataagtcgg cattttcatc
     4981 gattttaggg tcttttgtta ttttctaggt tggacatatc taaaaaattg tttctgacaa
     5041 taggagtagg aagaacgatg tgtgtctaaa tcctctgttg atggatagaa acctttatct
     5101 ttttcagttg tgaatcaggt atataccagt tataatagaa gaaacgaaag gacgggtgta
     5161 cgttatgtcg aatcaattac ctgtatatat acagattcat gaccaattaa aaaatgaaat
     5221 cgaacaaggt atatggaaag tcggagaccg tcttccttca gaaagagaat tagcagtgaa
     5281 attcaatgtc agccgtatga cgttgagaca agcaattcaa aacttggcag atgaaggaat
     5341 cttagaaaga aagatcggtt cgggtacata tgttgcacga gaaaaagtac aagaaaaaat
     5401 gtctggtgca acaagtttta cagaaattat ggaatcacaa aatcgagtac cgtctagtcg
     5461 gacaatctct tatttcttga cttcgccaag ctcaagtgaa atggaaaaac taaacttgag
     5521 taaagatgac acgatcttgc gaatggaacg tattcgtttc gcagatgatg tccctatttg
     5581 ttttgaagtt gccagtgtgc cacaaaagct gatcgatggc tacagcaaag cagagattac
     5641 tgaatcgttg tatcgcacgc tagaagaaaa aggcgggaaa aagatcggtc cagcgaatca
     5701 aacgattact gctgtgttgg cttctgagaa aattgctgac tatttagagg taaaacgagg
     5761 agatgcagtt cttcgtttgc gccagatttc ttattttgaa gacggtacgc cgtttgagta
     5821 tgtgcgtacg caatacgtag gaaatcgttt tgagttttat ttggaaaaat aaaaaacagg
     5881 tgatccctga ttccgtatta gaaataaaag agaatcagaa tcacctgttt ttatatatga
     5941 tcagttacca gttgctcgtg ataagaaaca ggaccctctt caaattggac ttgtccgttc
     6001 agcagctcaa tgatggattc tttaaagtgg tcagcacttt tttcatctac agcacagatg
     6061 acgctaactt gatcagtata ttggatttct tttataaaaa aatgttggag ttcaagataa
     6121 ttctgtaatt ttcctagttg cggatacgtg atcatcacag agatctcctg ttgcaaggta
     6181 ccttctacga ttccaatgtc agacaatgct tgagagaccg catgagtgta tgctcgtatt
     6241 aatccgcctg tgccgagttt cgttcctcca aaatatcgag tgactactgc agctacattg
     6301 atcagttgat tttttttgag gacttccaac atcggtatgc ccgccgttcc acttggttct
     6361 ccatcatcgc tgcttcgctg gatttcattt ttttctccaa tgatgaaggc gctgcagtta
     6421 tggttcgctt tccaatgttc tttttttatt tcttgaatga aactttttgc ttcgtcctct
     6481 gattctattc gtctaagaga acagataaag cgtgattttt tgatctcgat ctcactttgt
     6541 ccattttctt ttattgtacg atagcgctcg atcatcttgt cacctctttt ttcattttct
     6601 ttattcagta taaataaagt tctaggttgt ttcaacactg taacggttgc gcaattgctg
     6661 tagtatgcga taataaagac aaaggcaggt ggatctgggg atgcaggaac gaatgaaaaa
     6721 gtacgatgca attacgcatt atttgaagaa caatggcggc tctcaagtaa cgttgacctt
     6781 tacccaattt gacgaactgc tgtttcccag taacggactt cccaaaacag caagggaaag
     6841 tacagattgg tgggcaaatg attataaaca ccctgaaaaa ggtgcctatg gctggataaa
     6901 tgcaggctat gaagtagttg tcatcaattt ggacaaagaa tatgtcgtgt tcaataaatt
     6961 agtgaaatct agttggctat ttgattaaat aaaccgcgtt tagaaaacgt tgacaaattt
     7021 ctttcttttt atataataga gtaacagacg cgaagaaaac gtttgcgtag tttgttttaa
     7081 aaaggagaga aaagtatggc taaaaaaaca atcatgcttg tatgttcggc tggtatgagc
     7141 acaagtttac tagtaacaaa aatgcaaaaa gctgctgaag caaaaggaat cgactctgat
     7201 attttcgctg tgtccgcttc tgacgcagat aacaatcttg caaataaaga cgtggatgtg
     7261 ttgttattag gcccacaagt tcgctttatg aaagctgatt ttgagaaacg tctggaacca
     7321 aaaggcattc ctttagatgt catcaatatg gctgattatg gtatgatgaa tggcgaaaaa
     7381 gtgttagatc aagcaatcac gctcataggt gaataaatag aacagtataa ctatcgaaca
     7441 agcgatccaa aaaatagatc gcttgttttt tcgtatattt ttttagggcg aataatatga
     7501 atcatttttg gggtagaagg cttctgacga gagatattaa agcaatagac tgtgaggagg
     7561 taaatttacc aaattatgag aagatcgcag cagtggaacg tttgagcaac tggattcttt
     7621 gtcatcgctg tggaatgaga acgtccgtca ttgatggaca gcttgcagat tacggttatt
     7681 tttgtacaca ttgtctatat ttagggcgat gcgacactca acaagattta tatttgtttg
     7741 atcagcctga agcaaaacct cgagaagtcg tctttttttg gacaggacag cttactgaaa
     7801 aacaaacgga aatctcaaag agaatacttt gccattcaga aaaaagacat catcttattt
     7861 gggctgtaac aggtgcaggg aaaacagaga tgctttatcc tattttagtg gaaactttaa
     7921 aagctggagg aagagtagcg atttgtacac cgcgagtcga tgtttgtaat gaattgtttt
     7981 tacgctatcg ttatgttttt ccagaagaaa aaatcacgtt gctccatggg aatacagcaa
     8041 cggcttatac ctttacttct tttgttatct gtacgattca tcagctatac cgtttttatc
     8101 ggtcctttga tttaatagtg atcgatgaga tcgatgcgtt tccttataac ggggatgcag
     8161 gattagctca tgctgtgcaa acagcgatta aaccagacgg acgattcatc tacttaagtg
     8221 ccacacctga taaaaagtta ctaaaggaga tcaagcagac gtttgacatc cacaaacttg
     8281 ctttacgctt tcatcggcgt ttgcttcctg aacccgtctt ggttttcatg aataattggc
     8341 gacaaaaatg caagaacaag aagaagataa aatctttggt gcggatcatt catcaattaa
     8401 ttaaagaaaa ccatatcctt ttattttgtc caagtatcac gatgatgcat cgtttgaaag
     8461 ccacacttca agaggctctt ccgagatatg agatcacaga tgtttcttca agagacaatc
     8521 agcgggcgga aaaagtccag aaaatgcgag aagcaaaata tgacatactg ttaaccacga
     8581 tgatccttga acgtggagtc acatttgaaa gaatatccgt tatcgtttta ggagcggatc
     8641 atccggtttt tactaaatct tctttggtac aaatcgcagg aagagccgat cgtaaagggc
     8701 cttatacgaa cagtcaagta tattttttct atgaagaaaa aactgcagct attcgaaaag
     8761 cttgtcagga aatcaaagaa atgaatgaag aagggaagaa gatactgtga ggtgcagttg
     8821 ctgcagtaaa gatatcgtcc aaaatttaac attcagcgag attattggtg tttcgaaata
     8881 cagattacct cagacttgtt cagtttgtcg cgcacaattt catttattgg aaacaagagg
     8941 ctgttctggc tgtcaaaaac caatccattt caatctagag agtacgaaaa agaaagtaaa
     9001 ttctatcccc gtttactgca gtgactgtca agaatggaag aagaaatatc cgacctatga
     9061 ttttcagcac cgtgcctttt ttgtgtacga tgaaaatatg aaggcctggc tcaatcaata
     9121 caagtttatg ggagatatcc gactagcagg cacctttgca tataaatgga cgcagataaa
     9181 aaaagaatac ccgacataca tctattgtcc aataccttta tctgaaaaaa ggcgaacaga
     9241 aagaggattc aatcaagtaa gcgagatgtt gaaagcagca agaattcctt ttcagatgct
     9301 actcaaaaga gagaagcatc tgctggctca agcaaaaaac acgcgaaagg aaagattgga
     9361 gatgcctcaa ccttttgaac ttgcagtcac cttttctaag atacaaaata aaaaaatatt
     9421 gattattgat gatgtctata caacaggaca gactcttttc cacgcagcag attgtttgca
     9481 gatcgcacag ccgaaaatca tccgaactat ttctttggcg agatgaatta gatgtggaaa
     9541 tttgatagcg atttaatttg caaataacct agagttcgat ataatagaat taagaagagg
     9601 gaaagagtgg tccttctcct tcttgattgt ggtagacgaa aggggtaatt tttatgttta
     9661 gatataatgt acgcggcgaa aatatcgaag taactgaagc gattcgcgac tacgttgaga
     9721 aaaaagtagg caaactagaa cgctatttca gtgacgcacc cgatgcaacg gcgcatgtga
     9781 acttaaaagt ttatacagaa aaaacagcaa aagtggaagt aacgatccca ttaccttatc
     9841 ttgtattacg tgccgaagaa acttctccag atttatacgc aagtatcgat ttggttgttg
     9901 acaaattgga acgccaaatc cgcaaattca aaacaaaaat taatcgtaaa tcccgtgaaa
     9961 cgggcatgaa tactgcaaat gctgctgctt tattcaatga agaggacgaa aacgaaaatg
    10021 attctgaatt agatatcgta cgtacgaaac gcctttcatt gaaaccaatg gatagtgaag
    10081 aagcagtctt gcaaatgaac atgctgggac ataacttctt tatttttgaa gatgcagaaa
    10141 caaatggtac aagtatcgtt taccgccgta aagatggaaa atacggactg attgaaacag
    10201 actgatcagc cttacttaaa taacaacgaa aacttgcagc agctactgac agctgccgca
    10261 agtttttttg agtttcctcc tcttttagat gaaatatgaa taaattatga agttcttact
    10321 tta
//
LOCUS       DAZKCY010000085        10306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000085 DAZKCY010000000
VERSION     DAZKCY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.086"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(368..1486)
                     /locus_tag="ACG8XC_002017"
     CDS             complement(368..1486)
                     /locus_tag="ACG8XC_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191701.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1719..1841
                     /locus_tag="ACG8XC_002018"
     CDS             1719..1841
                     /locus_tag="ACG8XC_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191702.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1957..3264
                     /locus_tag="ACG8XC_002019"
     CDS             1957..3264
                     /locus_tag="ACG8XC_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFP9191703.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="ACG8XC_002020"
     CDS             3383..4582
                     /locus_tag="ACG8XC_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP9191704.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="ACG8XC_002021"
     CDS             complement(4609..5877)
                     /locus_tag="ACG8XC_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFP9191705.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5932..5998)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6040..6686)
                     /locus_tag="ACG8XC_002022"
                     /pseudo
     CDS             complement(6040..6686)
                     /locus_tag="ACG8XC_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7094..7597)
                     /locus_tag="ACG8XC_002023"
     CDS             complement(7094..7597)
                     /locus_tag="ACG8XC_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9191706.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7720..8484)
                     /locus_tag="ACG8XC_002024"
     CDS             complement(7720..8484)
                     /locus_tag="ACG8XC_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFP9191707.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8592..8867
                     /locus_tag="ACG8XC_002025"
     CDS             8592..8867
                     /locus_tag="ACG8XC_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP9191708.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9305..10255
                     /gene="yidC"
                     /locus_tag="ACG8XC_002026"
     CDS             9305..10255
                     /gene="yidC"
                     /locus_tag="ACG8XC_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP9191709.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         3103 a         2165 c         1871 g         3167 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcagcactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta
     5941 ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc
     6001 attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg
     6061 ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc
     6121 atgacaaata taattcccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga
     6181 aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt
     6241 tggtgataaa gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt
     6301 tttctgcgta cctggatcat tccagccgat gtacgtatgt tcttgcaagt cgatttcatt
     6361 ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct
     6421 aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc
     6481 ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgattctgg cagttcgtga
     6541 tctaatatgt ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga
     6601 tcattgacag atgagtacgc atttaataac tgttctactt ctcttattat aaaaaaatat
     6661 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
     6721 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ccggactctt
     6781 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta
     6841 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
     6901 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
     6961 aaagctggca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag caattttcgt
     7021 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
     7081 ttggttttat ctattaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
     7141 tacttgctga cataggctga gcggcttctt tgactgcgca gtatttgtct acgaatgtta
     7201 cgatatatcc ttctttgtac ttaggcggtg caatcgttgc gccccacatg tgcttcaaaa
     7261 tgatatctcg ctcaagatcc gacaattcgg tgatcttttc tgcattcttt acagcaattc
     7321 ttggatggat atacgcatgt gtgccttcat caaatttcgt cgtacgccaa tcatagtaga
     7381 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
     7441 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
     7501 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tctacataag
     7561 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatctg ccaccttttt
     7621 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
     7681 cgccttggat aaaatagcca aagcttaata gttccttaat cataaagaaa aatgataaag
     7741 aacaatccct gccgcaaccc caacattcaa cgattccgca tttcctttca tcgggatata
     7801 tacgttctga tcagtcaaag acaagatttc ctgacttacc ccctgacctt cgttccccat
     7861 gattattgcg aagttatctg atttttctac tttgtctaat gcgtgtgctt cttcatttaa
     7921 ttctgtgcca tacactgtaa accctttgtc tttcattaaa gaaatcactt ctgtcaaatc
     7981 tttttgcaac acaggcaaat gataattgct tccctgcatg ctgcgcagca cctttgtact
     8041 ataaatatct gccgttcctt tgcctaataa aacaccagcc atatcaaatg catctgccgt
     8101 acggatcatc gtccctacat ttccaggatc ttgtacatta tctaacaaca gccatcctcc
     8161 tgaaaaatct ggcgtgcctt cagccgttaa cgccatcact gctaagattc cttgtggtgt
     8221 aggtagatcc gatacagcat ccatgacctc atccgaaact agaaaagtag aaatctcttt
     8281 gtttgcggcc agccagtctc cccattcttt catgcctcgc tgtgtcacaa aaagttcttt
     8341 tatttctgca tcagacgacg cagcttcttc aatcagatgg aacccttcca aaagatattc
     8401 ttccttttct tcccgatatt tctttttatg tagttttttt aattctttga ttcttggatt
     8461 ttttgctgat tggatctctt tcacattcct cacctcccca tgattatatc atggaatagg
     8521 tgcttgagct tgtttcatcc taaaaaaatc ggtatcatta atttataaaa taggaaagaa
     8581 aggtgacgaa catgcgcaaa gttaaaatga acgtccaagg acgcgtccaa ggcgtaggat
     8641 ttcgctacat gaccaagatg gtcgctgatc aactaggcgt gactggaacg gtaaaaaacg
     8701 aagatgacgg ctcagtcacg atcgaagcaa tcggcaatga cgacatcatc cagaaattta
     8761 ttgaagaagt caagaaatct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag
     8821 aagacccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtgaaaaag
     8881 ctggtctgca tcaagcatta aggacaggtt gtgaaaaata gctctccata tttttcaaca
     8941 ggctggactt aatgccgtag atgcaagctt ttgaacaccg tcgataaggt tattcttgag
     9001 gcgctttgcc tcaaaaatta aggacagcag tgaaaaagct gatctatcac ttgcagtgag
     9061 ttcatttgac tccgcttttt cgggcagaaa aaaagttttt agtttattaa agtaagaaag
     9121 aaacacttgg aatcattcta ctttttccta gtatttcttt cttgattttt tgtctttttt
     9181 tcaaaatttt tcgctacgta aaagaacgaa tatttatatg aaatttctat gaagttgatt
     9241 gaattccatg cgctttttta gtatagttaa gtttgtgtaa aaattataga agaggaagta
     9301 ttctatgaat aaatggaaaa actggttatt gggatccgga cttgtcagta tcctgctctt
     9361 tttatctggc tgtgtgcgga tggattcaaa cggaaaccct gacacttcgg gaatcgttta
     9421 tcgagtactc gtccatccta tgggccaagc gattacatat ctagttgaaa atttcaattg
     9481 gtcttatggc tgggcagtta ttgcaatgac tgtgatcgtc cgtatcatca tcttgccact
     9541 aggtattagt caatcgaaaa aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc
     9601 acaagttgaa gcagcccaac aaaaattgaa agctgcaaca acacgtgaag aacaaatggc
     9661 agcacaagca gaaatgcagc aagtataccg cgaaaacgga ttgagtatga ccggcggaat
     9721 cggctgtctg cctctattga tccaaatgcc gatcttctct gccttgtact ttacggcacg
     9781 ttatacagaa ggtatccggg aatcatcttt ctacggcatc gatttaggct caccaagtat
     9841 ggtacttgtt gccattgcag gggtcgctta tctgcttcaa ggatacatct caacgatcgg
     9901 tattcctgaa gaacagaaaa agacgatgcg tacgatgctg atcgtctctc ctgctatgat
     9961 cgtcttcatg tcgatcagtg cgccagccgg tgttacactt tactgggtag tcggtggtgt
    10021 gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg aaaccacgtt tgaaagcaca
    10081 agtggcagaa gagttgaaac aaaacccacc aaaacaagtc gtcactccaa gaattgatgt
    10141 gacagaatca gctaaaccag ctgacagcaa acaagcaacc aaacaaatca agcaacccaa
    10201 aaacagtaca aacaataaag gccgcaacgc aggaaagcag cagaagcgga agtagatagt
    10261 aaataagagt gtaataaaaa ttcattaaca gttttagcaa ttaaaa
//
LOCUS       DAZKCY010000086        10215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000086 DAZKCY010000000
VERSION     DAZKCY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.087"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1519)
                     /locus_tag="ACG8XC_002027"
     CDS             complement(11..1519)
                     /locus_tag="ACG8XC_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFP9191710.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1678..1869
                     /locus_tag="ACG8XC_002028"
     CDS             1678..1869
                     /locus_tag="ACG8XC_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191711.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2072..2989
                     /locus_tag="ACG8XC_002029"
     CDS             2072..2989
                     /locus_tag="ACG8XC_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFP9191712.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            2982..4055
                     /locus_tag="ACG8XC_002030"
     CDS             2982..4055
                     /locus_tag="ACG8XC_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191713.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4203..4721
                     /locus_tag="ACG8XC_002031"
     CDS             4203..4721
                     /locus_tag="ACG8XC_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191714.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4702..5094
                     /locus_tag="ACG8XC_002032"
     CDS             4702..5094
                     /locus_tag="ACG8XC_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFP9191715.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5170..6105
                     /locus_tag="ACG8XC_002033"
     CDS             5170..6105
                     /locus_tag="ACG8XC_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9191716.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6238..7017
                     /locus_tag="ACG8XC_002034"
     CDS             6238..7017
                     /locus_tag="ACG8XC_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP9191717.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7010..7327
                     /locus_tag="ACG8XC_002035"
     CDS             7010..7327
                     /locus_tag="ACG8XC_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP9191718.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7571..9181
                     /locus_tag="ACG8XC_002036"
     CDS             7571..9181
                     /locus_tag="ACG8XC_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9191719.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9338..10186
                     /locus_tag="ACG8XC_002037"
     CDS             9338..10186
                     /locus_tag="ACG8XC_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9191720.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         3210 a         1793 c         2065 g         3147 t
ORIGIN      
        1 taaacttttt ctattggtaa tatagatttt cggaagggta gacaggtgcg caggtaatat
       61 cgattcctag ttttttcaat gtttgttcat catttttatt gaccatcaca gtagagtggg
      121 cttgtgctcc ctcaagttca gctagtttat cataagcaac ttgagcgatt ggattcgtta
      181 ctgcactgat tgataaagcg atcaagactt cattgatgct tagtgcatcc aaagaggaat
      241 gaagtgtctt tgatttcaat ccttgtattg tttcaagaac taatggtgac aataaaggaa
      301 tgtcatctgc gatatgggct aaatgtttta ctgcatttaa gataactgcc gctgacgcat
      361 ccatcaatga acttgtacgc ccggtaatga tgacttcatc tggtaattcg attgcaatca
      421 cagcagaagg atcttttgta ggactttgcg cttgaatctt atctgcatag tcatgggcag
      481 ggactaccac gtttcggtct tcaggacgta aaccgacttc ttccataatc agtttgattc
      541 gattaactgc ttcttcgtcg atcaatcctt ttttgaagtc gcattctgtt tggaaataac
      601 gacggatgat ttcttgttta gaggcttctt ggacaacggt atcatccacg atcccaaatc
      661 ctacacgatt cacacccatg tcagtaggag attgataaac agattcttta ccagtaattt
      721 tttcaataat acgtttgatt acaggaaatg tctcaacatc ccgattatag ttcacagcta
      781 ctttattgta ggcatcaaag tggaaggaat cgatcatatt gacatctttt agatcgactg
      841 tggctgcttc ataagcaatg ttcagcggat gtttcaacgg aacattccat actgggaaag
      901 tttcgaactt ggagtaacca gctgcatgtc ctttttggct ttcatgatac agttggttca
      961 agcaagtagc gagctttcca cttcctgggc caggagctgt cactacaacg attggtttag
     1021 ttgtttcaat atactcattt tttccaaaac catcttctga gacgattttt tctacgttga
     1081 ctggatagtc ttcgatttct gagtgtttat agaccttgat gtttcgtctt tctagtttat
     1141 tgataaagac tttcgtggca ggctgtccac tatagcgagt gataacgaca ctgttgatta
     1201 ataagccata accacgtaat tcatcgatta accgcaagat atccatatca taagtaatcc
     1261 cataatcacc acggatctta ttacgttcaa tatctccagc atagacgcaa atcagtattt
     1321 cagcttgatc tttcaatttt tgcagcaatt tgattttcgc atcttcttca aatcctggaa
     1381 gtacacgttt cgcatgcttg tcgtcaacta acttcccacc aaattctaaa tataatttgt
     1441 catagtggtt cactcgttcc aaaatatacg ctgactgctc ttcaatatat ttttctgaat
     1501 caaaaccaat ttttttcact aggttccctc cttaagtact aaagcatttt ttcatttttt
     1561 tgaggattcg tcaactggaa gagcgaaatt cctcaaaaaa acaaagaaaa tggtttaaac
     1621 caacttgtct agatagttat tcattggtat gatagttttt gataaggaag gtgctacatg
     1681 aaaaaaagac agaaaaagaa aaacgcatat aaacactata tccgttcgat ttttaccggt
     1741 tatgaaagaa tgcttgaaga tcccgaattg gaacaattga cgtttactta tctaaacgaa
     1801 gaaacgcagc taactcgtga tgagcatcag cgcattcatt tcacaacaag agatctgcct
     1861 agtaaataaa aaataagata aaggtcatcg gattcgattc tgtcctatca gatggaaaga
     1921 ctagttgatc gaaaaaaagg ctgtaccaaa ttttggtgca gcctgtttta tggagcattg
     1981 cccgctcttt tttataacaa ttttttacag aaaatggtac tattgaagta ttaataagtg
     2041 aaaaacagat gagtaagagg aggcaagtag tatgaaagag ccagatacaa atgcaaatta
     2101 tgagccagac gaactagagg agacgaacgc ggatcaggat acaggacaag aatcgtcttc
     2161 tttttacgat atcaaagaaa aagcaagcat gaatctttcc caattattag aagacttaca
     2221 ggcttttgaa aaagctgtcc gcgaagaaga tatccctgag atctaccgtt tatacaatgg
     2281 ccgattgaat gaagaattaa aaagaagctc aaatgaaaat cacgagatcg atcaattgct
     2341 gatgcgtaag atccatgatc gttttttaca ggaatttcct tttatggaac aagtagaaaa
     2401 catctcgcca acgatgagct attacaaaat cggcacttat taccatgatc ggcccacggt
     2461 cggtattgat gcaagtctgc cagaaatttt tgttttgcct aagatcgatg aagagtggga
     2521 gaaatattcc caaccggagt caacggacta tgacaaaaaa atcaatgagc tagacgcaaa
     2581 ggtgatcact gctcagacag aaattgaacg tttagatgaa caaataaaag aaatcaatcg
     2641 acaaatgact gatttgaacg acaataaagg attcttgaat cgtaaaaaaa tcgaggaaga
     2701 aattcaagaa ttagagaaga agaagcaagt actatctaat gaaaaattag gctggcttcc
     2761 ttatattgag acgcctgaaa caatccagca gcaaaaagaa gcattgaagc aagaagcaag
     2821 agcagaccag cttcgtgcag ccatcgtaga aaaagaacaa aggcaaatca gacgctattt
     2881 cggtagcaaa gagggattcg gtcaagcaat ccatgaattc ttgatgaatt atcttggcaa
     2941 tgagaatcct aataatcaat ctaatgaagg aggttccgag tatgaatgaa cacgatgaag
     3001 cagaatggac aaaaaatgaa gaacaaacag aagatcatga agaaacgaac acttcattgg
     3061 aagaacaaac aagtaatcct ctggatgaca cagaagaaat ccctatgcta gaggaaacaa
     3121 aaatacatga aaaagaaaaa gtaactccga cgaatacgaa tactgcagaa aacacccatg
     3181 cttacgaaat aactgaaaat cccgggtatt tagaaggaaa agagcaagat catcaagata
     3241 tcgtcagttt gattgaaaca gcggaagcag aattagcgga tttacgtatg agaaaagatt
     3301 tgatcgaatc agattttcca caattattag aatcatatga tcgttttata ttgaatgaag
     3361 aaccaatctc cacgatcgga aagacaagcc tgatcgaata cttcacgtat gaattactga
     3421 aaccgttgaa tgatattggt ctggaacggt caaattcagc ttatgacaca tggaaaacaa
     3481 gacatttttc gatcaattat acgttgaatg gacaaaacga attagtcttt tcctttgtat
     3541 taccgaccat cgatcaacga taccaagtgg aatcgatgag tctcctaaac gtatcaccag
     3601 agacaatgga aatcaatgtc caagatgacc gggtgctttc tttgatccga cactggtcag
     3661 tggatcgagt attctctgca gggcagatca cgatattcaa tcacaaattg aatcaattat
     3721 tagcccatgc aagaacactt ggttttaccg ttaatcagac attactagac aatacgaagc
     3781 cacttcgttt gaatcttcag agcgaatttg aattgacgaa tgaagtattg gatgatattt
     3841 ttatcgtggc aatgaaaaat ccatcctacg atatggaaaa gatcaacgaa actacttatc
     3901 aagtacgatt agataaagga cagtctttaa cgataacaac aaacgaaaaa gatcaaacag
     3961 tcttgtcgat ctcatctgga gactacccac gatctgtcat tgatttcttt atcaattacg
     4021 aattcttggt cccattaatg gtgagaaagt cctgattgga taagttggat taccagatgt
     4081 cattcagata attctgttcc aaaggacttg cggcagttat ccagaataat tgtcacaagt
     4141 cttttctgta tgtactaaat ggacgtgaat aacttgctga tgaaggaggg atttcattag
     4201 gaatgtccac aaataaaata gcacagctta gcttattgtc tgctgcttgt gtagcaggta
     4261 ggatcgcttt tcaatttatt ccgaatttcc agccaatgac tgctattttt ttatttatag
     4321 tactttactt aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt gcgatcagca
     4381 gtttttattt tggtgttgga ccatgggtca tcggccaatg gatcagctat actgttgttc
     4441 tgagtttgtt ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta tggttaaagg
     4501 gagtctgttt ttttctggcg ggcatggttt acggaatagg tatgaccgtt tttgatacgc
     4561 tgttatatcg tttgcctcaa ccttggatct attaccttca aggggtgtcg tttgatttga
     4621 tgcacagtat aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc aaacgttttt
     4681 acaatcattc aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc
     4741 agtatactca ttttgacaag ttgcagtaca acctatacac aatcggcttc caaccaaaag
     4801 gaggaagtaa aaacatcgca aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt
     4861 tcggaaaaag aagtggtctt caaaaaaggc gatgatttac tgagtatttt gaaacgaaat
     4921 tttgaagtca aagaagataa cggcttcatc acttcgcttg aagggcactc acaggatgag
     4981 aaaaatcaac tctattggat gtttactgtg gacgggaaaa gtgctactac tggtgccaaa
     5041 gagatcaaat tgaatgatgg acaatcggtc attttcaatt tgtctaaact ataagtttca
     5101 taaaaaactg ttaaagggcg aatattgcca taattcagtg agttcagttg atgaaagaag
     5161 gaaaataaca tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg
     5221 gctaatattc tagttgctat cagcaaattt gtcggctatg caatcagtgg cagtgccgcc
     5281 atgctaaacg aaagcatcca cagtatcgtt gattgcagca atcagatttt gctgttattc
     5341 ggtgataaac aagccaataa aggacagagc gagttgcatc aatttggtga aggacgagca
     5401 aaatactttt tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg
     5461 gtgatggaag cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa tccgatcatt
     5521 gttatagcaa tactgttatt tggtttggta atcgaaggaa gttcgttacg cgtggcaatg
     5581 aaagaaatca aagaattgaa tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt
     5641 cgacacagtg aaatactgat tatttttacg gaagacttgt gtgctgtaat cggtttggtc
     5701 ttggctttag caggaacaat attgacttat gcaaccggga ttgctgcatt tgatgcgatc
     5761 agcggattgc tgatcggttt cttgctgatg ggagccgcca ttttcctagc aaaagaattt
     5821 tatagtctga tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat caagttagct
     5881 tttgaacgtc ctgagatcag tcggctgatc gatataaaaa cagttcattt aagtccaacg
     5941 gagattttag ttgcagcaaa agcagatatc gttggtaaca aagagaatga agcttactcg
     6001 gtcatcaatg atattgaaaa aaatatgcgt cagtctcttc ctgacaagaa gatgtatata
     6061 tacattgaaa cagataaata tgacccaaat tattctcgta aataaaacgt ttattcatga
     6121 attgaagcaa aaatgagaaa aatataatct tgtttttaaa taaaacggct acaatttagc
     6181 gaatttatgt tacaatgagt ccgctgttta tttgttgtaa ttaaaggaga tattttaatg
     6241 aacaaaaaga aaagtgcgcc atggcttcaa gctttgcatg cagtgatgcc actttgtata
     6301 agctatatac cagtaggatt agcttgcgga gtattgttgc aaaaagttgg atttgatcct
     6361 ctcttatcag gactgttatc gattctggtc ttttccggtg gcgcgcagtt tttagttgca
     6421 tctatgctta cgacgcaagc atcatttgca acgacattat tgatggtttt ctttttagaa
     6481 ttacgctata cattattagg agcaagtctt gccggtttta tgaaaaaaga aaagcggacg
     6541 tttttggctg tcttttctca atcactgaat gatgaaaact atgcggtgaa ctacttaaag
     6601 ttttcaactg acccttcctg gaataaaaga aaggctttat atgtcaattg gttctcaatg
     6661 actgcatggg ccggtagcaa tatgatcggt aattttttcg gctcggtgat tcgagtagat
     6721 gctgaccttg tccattttgc tttgacggcc atgttcattt tcatgtttat catgcagatg
     6781 aaaagcgcac tactgatctt tacaggttta ttctcaggcg tattaggggt cgtattcatg
     6841 gtattattcc aaaatacttt cggattgatt gcagcaacag tcattgcttc atttgccgga
     6901 tttatgctgg agaaggcatt caaaaaagaa aaagctaaga aactaagaga ccgcggtaaa
     6961 gatgtcaatg agccgctctt caacttcccg gaacaggagg ccaatcacca tgactaaact
     7021 atatttgcta cttttagttg cttgtctgtt catcgtggca tatatcccgc gcttgttccc
     7081 gatgctttat tttactcatc gaaaagtgcc atcctggttc agtgactgga tgaaatatgt
     7141 accggtggca ttatttgcgg cgttagcttt taaagatgtt ttcattactc atgaacatct
     7201 ggatattgct tggaatatca aaatagctgc aatgattcta gtagcaggcg tggcttacaa
     7261 aacacgttcg atggcgttgt cagttttgac aggacttgcg tctgtctttt tgctgtctat
     7321 gctttaaact gttttatttt attaaaaaca gagattgcga cataagttgt tcaaactgat
     7381 ctatccgaac tgcatttgca acagtcaatt ctggatgcaa gtttattcgt agggataaaa
     7441 aagattagtg ttggcttatg atgcaagctc ttttttattt taggaattag agagggattt
     7501 gtccttctat gattttttgc tataataggg aagatgttta ttttatgaaa gaatagttga
     7561 gggaatttaa atgaataaaa aattgatgca aggaacattt tggctaacct ttgctaatct
     7621 tctatgtaaa gttttagggg tagtctattt gattccctgg ctaagtatga tggggaacaa
     7681 tcaagatggt atgcttgcta cgacattgta taatgttggc tatcttcctt atggattgtt
     7741 cctgatgctt ggtacagtcg gatttccaaa tgcgattgca aaaaaagtag ctgtagccac
     7801 aaaagaagga gataacgctg cttgtcggct gatttttaga agtacgatca atatcatgtt
     7861 tgtcatcggt atcgcctctg ctgcactgat gtatttattt gctcctttac tagccggaat
     7921 cagtccgatt gctaacgtta ataatgggat actagctatc agaagtttat gcccatcttt
     7981 gattgctatc ccaattctta gtgcaatgag aggatatttc caaggaaaaa atgatctgcg
     8041 gccttatgga acttctttga ttatcgaaca ggctgtgcga gtgatcgtta tcttggctgg
     8101 tacttattac ttgcgcgtat tgactgacgg aaccatatta gaagctgtat tgatcagtac
     8161 agtcgcttct ttcttcggtg ggttagctgc gattatccat atgtatatcg tcggacgcag
     8221 aaaagacttc ttcgaactta aagatttttt gatttccagc cgttatttcg aaagagaaaa
     8281 ccgatctgcc tctgtctcta tcattcggga aacactaccg tttatttttg tcggctcagt
     8341 cattactatc gttcaaatga tcgaccaagt aacgatgaaa ccactgcttc actttttccg
     8401 tccagagatc gcagatcagc aactggaatt cttattcagc cgtgcttctg tcaatccaaa
     8461 taaattgaca ttgatcctga tctccatggt aggaacagta gctataagca gcttgccaat
     8521 attaagcacg atgaagaaaa aagatcggat acaaatcgaa aaaacagtag gagatagttt
     8581 ttcgattgcg ttattgattc ttcttccttc attgactgga atgtctttat tagctggacc
     8641 actatacaca ttgttttttg gctatgatcc tgaaagtgtc ggatatttcc aaatggcttt
     8701 gctagcttct ttgtttttct ctttattcac gatcctatct acgatgcttc aatctttgaa
     8761 tcaccatcgt tttgcgatcc gattaactgt agaagcaatt atcctgaaag tcatcttcca
     8821 agtaatcgga ttaggattag ttggcggata cgggatgagc ttatcaaatg gcttagcatt
     8881 cggtattgtc tttgttcgcg gctatcactt tatgtgcaaa gaataccgaa tagcaccttt
     8941 agcaaagatc agcaattttt tcttaaaaac tttccgctcc accctgatta tgcttgcagt
     9001 atgttttgcc gttttctttt tgctgaatca gcgattgtca atggaatcaa aaagctatgc
     9061 aatgatttat tgtgtggctg taggaagtat tggcggctta gtatttttat tttctcaatt
     9121 cggaaaaaat gggtttcgaa tgttgaaaaa tgttaaacat catagcagaa gcaaagaata
     9181 ggttaactaa tcgttttttg ctgtcacttg aaaaacggca actcgtatga ttacgcttgt
     9241 cgtttttcta ttttccaatt ttagtttcat atctaatctt acttatgata tagttataag
     9301 gtctggaaaa attctttgaa acgaggaaat taaacacatg aaacttgcag tagttacaga
     9361 tagttcggca tatctgccgg aacgtatcaa gaaccatccc gacttattcg ttattccaat
     9421 ccctttgatt atggatggga aaatttataa cgaaggaatc gatatcgaag cggatgaata
     9481 ttatggactt ttaaaaaaca gtaaagattt tcctactact tcacaacctg tcgtagggga
     9541 agttttgggt ttatatgagg aattgaaaga gaaagggtat gacacgattt taagtatcca
     9601 tctttcttca gggatttctg gctttgtcaa cacattattt gcgatgaaag atgacatcag
     9661 cggagtaacg gtcattcctt atgactctaa gatcactagt atgccgatgg gacacatggt
     9721 agaagctgcc ttagacttaa atgatgaagg tcagtccctt gatgaaatac ttgcacatat
     9781 cgatcgaatc cgagataata cctatgctta tctgattgta gatgatttga acaatttagt
     9841 acgaggagga cgtttgacaa atggtgcagc gctgattggc agcttattaa aaatcaaacc
     9901 gatcctgaca ttttctgaag ggaaaattgt tttattcgaa aaaatccgtt caagtaaaaa
     9961 agcatttgct cgagctgaag aaattattgg tagaagaaat aacgaaatac aaccgccagt
    10021 caaactttac gtgatacatg caaataattt agaagtagcg attgcggaga aagagaaatt
    10081 gcagaaaaaa tatccaaatg cagaaatcga aattggtcat ttcggaccag tcattggtac
    10141 acatttaggt gaaaaagcca tcggcattgc catttcagcg caataattcc ttttattcaa
    10201 aaaacatacc aaaaa
//
LOCUS       DAZKCY010000087        10192 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000087 DAZKCY010000000
VERSION     DAZKCY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.088"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..256
                     /locus_tag="ACG8XC_002038"
     CDS             107..256
                     /locus_tag="ACG8XC_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFP9191721.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            267..1781
                     /gene="dltA"
                     /locus_tag="ACG8XC_002039"
     CDS             267..1781
                     /gene="dltA"
                     /locus_tag="ACG8XC_002039"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFP9191722.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1778..2983
                     /gene="dltB"
                     /locus_tag="ACG8XC_002040"
     CDS             1778..2983
                     /gene="dltB"
                     /locus_tag="ACG8XC_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFP9191723.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3028..3261
                     /gene="dltC"
                     /locus_tag="ACG8XC_002041"
     CDS             3028..3261
                     /gene="dltC"
                     /locus_tag="ACG8XC_002041"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFP9191724.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3264..4532
                     /gene="dltD"
                     /locus_tag="ACG8XC_002042"
     CDS             3264..4532
                     /gene="dltD"
                     /locus_tag="ACG8XC_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFP9191725.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4673..5755
                     /locus_tag="ACG8XC_002043"
     CDS             4673..5755
                     /locus_tag="ACG8XC_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFP9191726.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5755..6558
                     /locus_tag="ACG8XC_002044"
     CDS             5755..6558
                     /locus_tag="ACG8XC_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP9191727.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6628..7368
                     /locus_tag="ACG8XC_002045"
     CDS             6628..7368
                     /locus_tag="ACG8XC_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9191728.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7379..8383
                     /locus_tag="ACG8XC_002046"
     CDS             7379..8383
                     /locus_tag="ACG8XC_002046"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFP9191729.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8428..10191)
                     /locus_tag="ACG8XC_002047"
     CDS             complement(8428..10191)
                     /locus_tag="ACG8XC_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191730.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3267 a         1693 c         2070 g         3162 t
ORIGIN      
        1 tgtgacaaaa ctgttagtaa aaaaacggtt tctgtaagct agacacatgt ttctttttgt
       61 caaaattagt tacactttgt gtgaggttat atgaggaggg aatccaatga acaaatggcg
      121 ttctaatcca aaattgaatt attggggaaa attcatcatt cgaacaattt tttatagtgc
      181 agtactgctg ttcctgattt atctgtatca ctataaaaat attcaaggag gaacttttat
      241 ctataacgaa ttttaggagg aagagtatgg aaatcaaaac gattattgaa gcaatcgatg
      301 attggggaat aaatgaacct gatcgagttg cttatcaggc ggatgagacg catacttttg
      361 gtgaattgaa acaagcatca gacgcacttg cttattattt agaaaaaaag gtagaaggcg
      421 atggaccaat tgtcgttttt ggcaatttgg agtttgaaat gattgtctca tttctagggg
      481 ttgtcaaatc tggccatgcc tatttgccga ttgaagagca tacacccaag gagcgtatcc
      541 tatcgatctt tcgggtggct aaaccatcta tggtcatcag tatcggggac tggatagaag
      601 atattccttc ggttcctgtc ataacaaaaa aagaatttca agaaatcaag aagataccag
      661 taggatttta tcccagaaat agcgtaaaag gtacagaaaa tttttatatt atttttactt
      721 ctggtactac tggagaacca aaaggcgtac aaatcagtca tgataatcta gtcagctttg
      781 ttcaatggac gatggaagac tttgggatca agcctggtat gcatttctta gcccaagcgc
      841 cattttcttt tgatttatcg gtattttcta tttatcctgc attagtttct ggcggaatgc
      901 tgaagccgtt aaataaagca gttgttcaag actttcgtca attattcgcg acattgccag
      961 ccttgaagtt gaatgtgtgg gtttctactc catcatttat ggatatttgc ttgatggaac
     1021 ctacattcaa tggagaaaat gtgccggaat tggacatgtt tttattttgc ggagaagaat
     1081 tgacgaaaaa aactgcagaa agtctacttg atcgattccc aaatgcacgg atatataata
     1141 cttacggtcc aacagaagca actgtagcta tttcaagtat tcagattgac cagcatgtac
     1201 tagatagtta tgaccgacta ccgattggct acgtaaaaaa agacacgaaa gtttctattg
     1261 tccaagaagg taaacctgtt tcaaaaggag aaacgggtga aatcattatt gcaggaccaa
     1321 gcgtatcaaa aggttacctg aacaatcccg aaaagacagc tgctgcgttc ttttgtcaaa
     1381 atgacttagc aagttaccat acaggggatg caggtagact agatgaatat gggcttttat
     1441 tttatgaagg acgtatggat cagcaggtca aattacatgg ataccggatt gaactcgggg
     1501 atatcgaaca ttatctgctc caagacaacc gaatcaaaca agctgtcgtc gtaccaaaat
     1561 accaaggaac aaaagtacag cagctggttg cttttgtcgt attagaaaac aaaacgaaac
     1621 agccggattt tcagttgact aaatctatca aagaacaatt actagaagtt gtaatggatt
     1681 atatgattcc tcagaagttc cattatgtcg atctgctgcc tcagacagtt aatggaaaaa
     1741 tcgatcgaaa aaaattaaca gctgaggtga atcctgaatg atgttacctc atatgatccc
     1801 ttatgcagag ccgttttatt tttttctttt agccattgcg cttgtaccga tcgttctatc
     1861 tttattgata tgggaaaaaa gattaccagt ttatcaatca gcagtgacac ttttcttttt
     1921 atttgtcagc tttggaggta attattggca acagggagcg gcgctgattg ggtatgtttt
     1981 gtggcagacg atacttgtat ggggctattt tgtttatcgg caaaggaaaa atcagacagg
     2041 atggtttttt gccgccgtta ttttagcgat aattccactg tttttaacga aagtgactcc
     2101 tttttttgaa catggaaaag catcgttact tggtttttta ggaatctctt atttaacctt
     2161 taaatctgta caaatgatca tggaaataag agatggcatg atcaaaacat ataagccatt
     2221 tcgttatatc cagtttctgc tgttctttcc aacaatctct tctggaccaa tcgatcgcta
     2281 taggagattt gaaaaagatc agctccatcc gcctgttcgt gataaatatg ttgatctttt
     2341 gggaaaaggc gtccaaaata tttttgtcgg ttttttatat aaattcatta tcggttatta
     2401 ttttggtcaa gtactgcttc ctgtagtggg acgcattgcc ttacagcatg gtggtatttc
     2461 atgggcatta gtaggttata tgtacgtgta cagtatgtat ttgttttttg attttgctgg
     2521 ctatagctta tttgcagtcg gtacaagcta catgatggga tatcagacac caatcaattt
     2581 caataaacct tttttatcat ggaatatcaa agaattctgg aatcgttggc atatgacgct
     2641 gtccttttgg ttccgtgatt atgtatatat gcgattgatg ttcttcatga tgaagaaaaa
     2701 attttttaaa agcaagatcg taatgtcaaa tatcggttat tttgctttgt ttcttctaat
     2761 gggtatatgg catggattga cgtggtacta catcgtatat ggactgtatc atgcttgtct
     2821 gatctgtctg acagatgcat ggatccgatt taagaagaag cataaagagc agttgccttc
     2881 taacaaattt acgcatggct tagccgtatt tttgacattt caagcagtat gttttagctt
     2941 cctgattttt tcaggatttt tagataaact tttttttagc taacgaatag tgacatatta
     3001 atttatataa caaaggagaa aaggaaaatg gaagaacaaa ttttatcaat cttagaagaa
     3061 ataacaggaa cagatgaagt aagaaaaaat cgtgatattg atctatttga tgaagggttg
     3121 cttgattctt tagcatctgt tcaattatta gtcgagctag acggagagct ggatatccaa
     3181 gtccctgtat ctgaattcga gcgagaggat tggagcacac cgaataaaat cattcaacaa
     3241 gcaactgcat tgaagggttg agtatgaaaa agaaactttt tgctattttc ggcccggttg
     3301 ttttagctgt tggcttactg tttgtgtttt ttacctcctc ctatcaagta gatatctcta
     3361 atccagaaac gattagacgt gcctcttctt ccatgtcagt gaacgtgttg aaaggagatg
     3421 cgataaaaaa ttatgcatta tctgaaaaac aatatattcc tttttttggc tcaagtgaat
     3481 taagcagaat cagtccattt catccgtctg ttctggcaga aaaataccag cgaaactatc
     3541 gtccattttt gctgggagca cccgggacac aatcgttaag ccaatatatg atgatgcgtt
     3601 ctgcaggaga tgctatgaaa aacaagaaag tcgtctttat tatttctcct cagtggttcg
     3661 ttaaaaatgg tgtaaaaact gattacttta atacgtatta ttctgaactt caaacatacg
     3721 actggttgtt ttcaatgaag aaagtaacgc ctgctgatcg ctatttagct agaagattat
     3781 taactttctc aaaagtaaaa gagaatgata cgttgacggc gattctgcaa acgatcaaaa
     3841 aagggaagct acctttacct gaatctttga atcagctgcg cagccaatgg aacatgctga
     3901 agagagagga tgaagtattc agcaatattg ggttgaaaga tcgacagcaa aaaatcgatc
     3961 atgagtcaaa acgattacca aaacaatacc aagaaacaga attatccatt ttagcaaatc
     4021 aaattggaga acgagaaacg acgaataatc cattcgggtt gaaaaacgat ttttatacgc
     4081 atcgtatccg agcccacgag ccagagctca aacagtcaca aaaaaattgg gattatcgtt
     4141 tttcgccaga attttctgat tttcagctgg tcttggatca attagcaaaa aaccataacg
     4201 aagtattgtt catcatccca ccggttaacg agaaatggag cgattatacc ggactatctc
     4261 aagaaatgtt acaaggattt gccaaaaaga tcaaatttca attaaatagc caaggattca
     4321 atcgaatagc tgattttgtc aatcaagcgg gtaccaacta ttttatggaa gacacgatcc
     4381 atctaggctg gaaaggctgg ttggctgcag atcaacagat cagaccgttt ttagaagaaa
     4441 atcatattac tgctagtaag tatcatcttg acgatgcttt ttttagcaaa tcttggcaac
     4501 atcaaatacc agataaacta caattaaaat aaagaaaaca ggcgagtgtt gctcgcttgt
     4561 tttctttatt tttgaatatt ctgaataaaa gataaaaata ccaagtttgc ctgaacaaaa
     4621 atcataagca atggtataat cacaatatga atatagaaat gggggaaaac atttggatac
     4681 aaaagaagaa cagctactta ttgatttaac ttctgcaaaa ggagtacctg gaaatgaaga
     4741 agaggtaaga gaagtattca gagaatatgc caaaccattc gctgatgata ttttttatga
     4801 cggtttaggc agtgtgattg ccaaacatgg tgctggcgga ggccccaagg tctttatttc
     4861 tggacatatg gacgaagttg gttttatggt aacaaaaata accgaaaaag gattcttgga
     4921 attccaaact cttggaggtt ggtgggggca agtcatgctt gctcagcaag ttgaaatcaa
     4981 aacacgtgaa gggaaaatcg tccacggtgt gattggttcc aaaccaccac atgttttaac
     5041 acctcaagta agaaataaac cttatgaaat aaaggatatg ttcattgata ttggcgcatc
     5101 aagccaagaa gaagccaaag agtggggcat acgtcctgga gatatggtca ctccatatat
     5161 tgaatacaaa cgaatgaatg gcagtaaata tttactggca aaagcatggg ataatcgtat
     5221 cgggacagct gtttctctgc gagtattaga gaatctatct aaagaagcac acccaaatgt
     5281 tttattcgca ggaagcgatg tgcaagaaga agtaggcttg cgaggtgctc gaacaagtac
     5341 acaccttgta aatcccgata ttgcttttgc tttagatact ggtacagcag gagacactcc
     5401 aggaatgaca ccaaaagaag cagattcgat tttaggaaaa ggcccacaga ttttgatttt
     5461 tgatgcatct atgatcccgc ataaaaaatt attgaatttt gttattgatg tagcagagga
     5521 aatggatatt ccattccaat atacagtgat ttctggcgga ggaactgatg caggacaaat
     5581 gcatttgaca agagatggtg ttccgtctgt agccattacc gttcctgttc gttatctgca
     5641 ttcccataca tcagtgattc atgaagatga ttatttgaat acagtgaaac tagtcacaga
     5701 attagtgaaa cggcttgata gtgaaacagt tgaaaaaatt cggagttatg aataatgaat
     5761 aatagaatac ctatttctga atatcggaaa aaagcacgag tttctttgga aaatcaatgg
     5821 ggaatcaata cttggcttgt tttcttatct ataatattta cgagcgtcat ccagaacttt
     5881 tttgacggtt tcagtccttt tccggaagaa agcatgcagc agcacttatt gagttttcta
     5941 gtcgataatt tacttttatt cgcattcaac tatgcattgt tttacattgc gctagtagtt
     6001 gtacggggtg gcagaccaaa aagtaacctt ttattttcag ttttccaaag aaagtatttt
     6061 ggttcgatac ttggcatcaa tttgatcaat tcagtcatca actggctact caacagttta
     6121 atctttctgc ccatgttttt aatcagcgga gtgagtactt atatgcagct gcttttcagt
     6181 tcaaatgcgg tacctacggg tgtcatgcca gaagattttc ttgatatcag ctttgtttta
     6241 tcgatgatag ggatgatttt tgtttcagca ctcatcatga gtattgttgg cggtttattc
     6301 caatttgctg catggacaaa gttcgactat cctgatttgt cggttattca atgtttgaaa
     6361 tatggctggt ttttattgaa agatagaatt ggtacgtata ttttgttgca gctgtctttt
     6421 atcggctggt atttgctagg agcaatcgct ttgttcatcg gtcttttgtg ggtcattgtt
     6481 tatgtgaatg tgtcagttgc agaattttat gaacaagcac ggattgaaaa aggaggtcca
     6541 gacgcctatt ttacttagca tgtaagcagt ttattcatca aaaaaataac ttttgtggta
     6601 ggataaatct ttgaggggga atgtacgatg aaattaactg ttttaggatg cttaggagcg
     6661 tatccttata aaaatcaagg gacaacaggt tatttgcttc aatctgacgg gttcaatttg
     6721 ttgatcgatg caggtagtgc tacgctgatc aaattacaag aacaccttga tccacttgat
     6781 ctagatgctg tgatccttag ccattatcat catgatcata ttgctgacct tggtgtatta
     6841 cagtattact ggcaacttca tcctaagaga aatccggaga cattgctttc aatttatgga
     6901 cacacaaaag attccattca ctttgaagaa ttgacgttgg aaaatgtctc aaaaggacat
     6961 ccttattttg aagctgaaga attaaaaatt ggtccttttc tgattacttt tatggaaaca
     7021 atccatccag tagtatgtta cgccatgcgt atcgtagaag aaacaacggg gaaagtattc
     7081 gtatttacag gtgattcagg ttatttggaa agtttcgctg attttgcgaa agatgctgat
     7141 ttgtttctag cagataccta tctttttgaa ggaaatgaac atcatcatgc ccatttcact
     7201 tcaaaagaag ctggagaact tgcgaaatca gctaacgtaa agaaacttgt acttacacac
     7261 ctacctcagt ttggtgattt agaacaattg aaaaatgaag cgcaaaaggc agcaggcaaa
     7321 gggattgata tagaacttgc tgcagttgac aaagtgttta acatttagga ggaagaacat
     7381 gatttttgta ccaaatgaaa accacgaccc aagaatcaat ttagcaattg aacaatattt
     7441 gctgcaagaa atgcctttag atgaacctat tttgctcttt tatatcaatg aaccttcaat
     7501 catcatcggc agaaaccaaa atacgcttga agaaatcaat cgagattatg tggaagaaaa
     7561 tggcattcat gttgtccgtc gcttaagtgg aggcggagct gtctaccatg attttggaaa
     7621 cctgaacttt agttttatca tgcctgatga tggggattca ttccgtgact ttgcgaaagt
     7681 aacacagcca atcatccaag cacttcatga attgggtgtt gctggggcgg aattgaaagg
     7741 ccgaaatgat ctagtcattg atggcatgaa attttctggc aacgcgatgt atgctaccaa
     7801 cggtcggatg tttgctcatg gaacaattat gttcgactct gatatcaatg aagtagtcaa
     7861 tgcattaaaa gtaaaaaaag acaaaatcga atcaaaagga atcaaatcga tccgttctcg
     7921 tgtaacgaat atcaaaccat ttcttccaga agaaaagcaa aaaatgacaa cagaagaatt
     7981 tcgtcaagaa atcttgttga aaattttcgg cgtagagtca gtagaagaag taaaaacgta
     8041 cgaattgact gatgaagatt ggaaaaaaat caatcgaatc tctgaggaat attatcgaaa
     8101 ttgggattgg aattacggga agtctccaga tttcaatttc tctcgccaaa aacgtttttc
     8161 tatcggttcg attgaagtcc atttgaatgt ctcagaagga atgatcaaag aagcaaaaat
     8221 ctttggcgat ttcttcggac taggagatat ctctgacgtg gaaaaaaaat tggttggaca
     8281 gaaatatgat aaagcatctc tatcaaaaat agtcagccaa attgatgtga agaaatattt
     8341 tggtgcgata gaaccagaag aattgcttac cttactttat tgaaaataaa aaatagcgga
     8401 atccacatat atggattccg ctatttttta tacaggattc gggtaatttc gagagagttg
     8461 aacagtgatc cgatcaatga aagtatcttt ttcctgcgtt agtgttcctg tgtaacgatc
     8521 cgctacgtct ttcatcatca ttaagtcaaa acgttcctga tcatctaatt cttctgtgat
     8581 gcctcgacta aataggtttg tatcgtaatg tgcactatgt tcgcaacaaa tgacgaaatg
     8641 ttcctcttgt tcgtgtatcg acaaataaat ttttcgatac agtggatttt cgatggcatg
     8701 agtttctcta aaggaatgct ctaaagcgtg gatcaaaagt tgagccagat cgtcatcgtt
     8761 gataggaagt tcttcgggaa gatctgcttg gaaggttatc tgaatattcc gtttggcagc
     8821 taacttctgg taacgcgcta agataagatt tgtcacctgg tggttgcaaa aagaacacag
     8881 catttcgaaa ttttttgctt cttctaatat cttttccaca tagtttttag cttttgtttg
     8941 gtccgtatgc attaaatctt gcagattctc catctcttgg acaaattcca ggcgaagtgt
     9001 atttacttct tggatatgct gtcgaagctg ctcataatgt tgctccaaca aatcattttt
     9061 gatttttaag aaatctactt gtctccgatc tttgtcggta tttaagatgt gatcgattag
     9121 attgtagctg ataataacga ttagaatgat aatatataaa atcgtagacc aattgatcgt
     9181 tgtttgatag atacctaatg ccgtttggat gtaaatcaag caatggataa tggctaccaa
     9241 gacgatccaa ggtgtacaag ctacataaaa acggttattc ttatatgctt cgccaatgca
     9301 tgcaagtgaa gtgactaaag tgcttataac actagcaata ttagcaatcg gcattaattc
     9361 aggaagttct gcaggagtaa aagttgcata aagcagaacg ccgataaaaa tcaaaagttg
     9421 gaacgtacaa aaaattccgt accatttttt atagtaaaac attcggctcc aatagtagca
     9481 gatgatgaaa gctgacgtta agataataaa taaatgagat aaaaaagaat gaacgaatgg
     9541 ttcaaaaaga ataccactta aaatatcttc tgatacagct tcaaatccta atgccaaagc
     9601 aaagcaactt aagagaataa gtgagtattc aagacgtttt tgtttataca tcaagtagat
     9661 cgtatagaaa aaggtcccta tagcgataaa aacagcgatt atcatcgtca atacttgcgg
     9721 cactgattgt gaataaataa aactaatcaa tggtccagtt gttcctagat aaactttcgg
     9781 tggcagtcca gcatagttct gataaggaga tgagactgta agggtcaatt tttttccgat
     9841 ataatttgct ggtaaatcta tcacagccaa tgagagtccg ggattgcttt cttcctgata
     9901 atctttccgt tggtataaaa tcttttcgcc tagatataca gtaatccatt gatggtttcc
     9961 tttgattagt aaatttgctt cgtccaacgg tttttcaagt gtcgtttcca taatgaatgt
    10021 ttcgtcttta tccacggtag gaatcgtacg agtgtacttg ctgtcaaact taaaatcagg
    10081 atcatctttt gtatagaaaa cccacgaatc ttttatttct ttcatttgat tgctgtccgc
    10141 aggagaaaaa ataaaataaa agaaataagc aattcctgtt aaaaataaca at
//
LOCUS       DAZKCY010000088        10172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000088 DAZKCY010000000
VERSION     DAZKCY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.089"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..719)
                     /locus_tag="ACG8XC_002048"
     CDS             complement(6..719)
                     /locus_tag="ACG8XC_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9191731.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            894..2318
                     /locus_tag="ACG8XC_002049"
     CDS             894..2318
                     /locus_tag="ACG8XC_002049"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9191732.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2617..3324
                     /locus_tag="ACG8XC_002050"
     CDS             2617..3324
                     /locus_tag="ACG8XC_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP9191733.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3311..3634
                     /locus_tag="ACG8XC_002051"
     CDS             3311..3634
                     /locus_tag="ACG8XC_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP9191734.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3723..4760)
                     /locus_tag="ACG8XC_002052"
     CDS             complement(3723..4760)
                     /locus_tag="ACG8XC_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9191735.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(5081..6064)
                     /locus_tag="ACG8XC_002053"
     CDS             complement(5081..6064)
                     /locus_tag="ACG8XC_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFP9191736.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6479..7249)
                     /locus_tag="ACG8XC_002054"
     CDS             complement(6479..7249)
                     /locus_tag="ACG8XC_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9191737.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7242..8177)
                     /locus_tag="ACG8XC_002055"
     CDS             complement(7242..8177)
                     /locus_tag="ACG8XC_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9191738.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8493..10142)
                     /locus_tag="ACG8XC_002056"
     CDS             complement(8493..10142)
                     /locus_tag="ACG8XC_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9191739.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
BASE COUNT         3004 a         2122 c         1813 g         3233 t
ORIGIN      
        1 cgagcttatt tttcaacaaa aacagcttca aaaacaaagt cttgatataa atgtctcgat
       61 accgaatact cgaaaggttt accagacgat agagcaacaa ctttgtctat ctcaatcata
      121 aattcacctg gatctatttt taatattttt gcgtcaaatt cattcgcagt atctcctttg
      181 atacgtatgt gcgaagtctt tatttccaga cccagttcat ttgtgatata agcatataca
      241 gatttcttta aatgttttgg ttccaagcct ggtatgactg ctaatggcat gaatgtttgc
      301 tcaatcgagt atggttgaca atcaataaaa cggcttcgta caatattata tacatagtct
      361 tttacactga tacttaattt atcagctact tttttcgatg gaagttcaat tgagaaagta
      421 tggatttctg aatctacgtg ttcttcacca accacgctaa acaaaccttc gtttggacca
      481 agaactcgtg agttatcaat tggtgttctg acgacttcag tcccataccc acttctactt
      541 cgcaaaactc cttcggaaac aagtaagtct acgcctttct ttactgttaa cttgcttgta
      601 tgataaataa ctgccaattc atctcctgta ggaagtttct caccaatttt atactctcca
      661 tttacaattt ttttctttat atctaagtaa atttccatat acttcgcaat ttttttcaat
      721 ggtgaccctc caatcaaaaa catatcttca tctatacttt aacataaata aaccgataaa
      781 atccacatca aaattataca cacaataaaa aagatatata tatttaaaca aaactattta
      841 ctttaatttg taagcggtgt tatcattaag tggaaacagg aggtaaaaaa attatgacaa
      901 ctatgaaaaa gctaactttt ccagatgatt tttggtgggg ttccgcttgg tcagctgagc
      961 aagcagaagg aaaaggagaa acaggaaaag cagagactat ctgggatcgc tggtttaaag
     1021 aacaacctta ccggttttat caacgaatcg attcctctat tacaaccgat cacatccatc
     1081 aatataaaga agatatagaa ctaatgaaaa aaactgggca taactccttt cgcgtttcta
     1141 tttcttgggc acgaatgttt ccaaatggcg gtaccggaaa agtaaatccc aaagccattt
     1201 ctttctatcg aaaattattt tccgaaatga ataataacgg aatcaaagta tttgccaact
     1261 tatatcattt tgacatgcct gctactcttc aagataaagg aggatgggaa tcacgtgaag
     1321 tagttgatgc ttatgttcat tacgctgaaa cttgttttaa agaatttggt gacttagttt
     1381 gtcattggtt taccttcaat gaaccacttg ggcctatttt aggtacttat cttgaagatt
     1441 ttcattatcc aaacctcatt gactttaaac gcggagcgca agcagcattc ttcactatac
     1501 ttgcacacgc aaaagcaata caagtattta aagaactagc tcttcctagt aaaataggcg
     1561 ttattctaaa tttgagtcct acttttcctc gtagtcaaaa tcctgctgat gtgaaagcag
     1621 cagaaatcgc tgatttattt tatacgcgga gctttttaga tcctatggtt aaagggaatt
     1681 tttctaaaaa gcttatcctg ttattacaag aatataatca gcttcctgaa gattatacga
     1741 aagaagatct tgtatgtatc tacgaaaata ccgctcaaat tcttggacta aactattacg
     1801 agccacgtcg cgtcaaatct cgttcttcta tggtggatac atctagtcca tttttaccag
     1861 agtggttttt cgaaccacat atcatgcctg gacgacgaat gaacgaatac cggggatggg
     1921 aaatttacga aaaaggaatt tatgatttgt gcatggatat caagaaccat tatggaaaca
     1981 tcgaagcatt tatctcggag aacggaatgg gcgttgcgga tgaagaacgt ttcatggatg
     2041 atacagggca aataattgat gattatcgga ttgagtatat caaagatcat cttgcttact
     2101 tatggaaggc aataaatgaa ggatgtaaca ttaaggggta tcatctttgg acatttattg
     2161 attgctggtc atggatcaac gcctataaaa accgatatgg gctggtttca ctcgacctta
     2221 ctactcaaaa aagaacaatt aaaaaaagtg gcgaatttta caagcaatta agtgatacaa
     2281 atggatttag atacgatgta gacttgttag ttgaataata ttctaaaatt ccacttgaag
     2341 tgaacacatt agaaaacaag aataatgaga aataccaata acaaatactt tttcttcata
     2401 cttcatttat acttgtcagt ataagagact gtgacaaatg tagtgatcct cagaagttag
     2461 gtctggaaat ctaacttgag aagatttttt taaactacgt ccagtctttt ttatttgttt
     2521 aaaattgaag ttgacaagtc ttcattaaaa atttagaata ttcacaagtt tgacgacaat
     2581 tatgaaaatt tatgtcgtgc taaaggagtt ttgttattgg atgaaaagtt agatataaaa
     2641 actgcgatca aagacacgtt acctaccgtt tttggttata tcggtattgg acttgcattt
     2701 ggtatcgttg ggaaagctgc cgggtttcat ccgctggtcg tcacgttgat gtccctgctg
     2761 atctatgctg gttctgccca atttatcacg gtcagcatgc ttgctagcca tagtccactg
     2821 ctctccatcg ttctctcgac ctttctagtc aattcccgaa tgattctgat gagtatgacg
     2881 attgctcctt atttcaagaa aaatcggctg cttcaaaatc tgctgattgg cacattgctg
     2941 acagatgaaa gtttcgcact gggaatgaat aaattgaact atactggaca aaagttaaac
     3001 tttcgttgga tgaatacagc taattggatt tcttatttga cgtgggtggg ttcttctctt
     3061 gttggcgcac ttttagggaa ttttattact gatccgaaaa agtttggttt ggatttcgcc
     3121 atcgtcgcta tgtttatcgg gttactttat cttcaagtca tttctgatag aaacacctct
     3181 aaacgattac agttgatcct gattggtctc actttgattc tggtatatgt cggactgatt
     3241 tttatcccaa gtaacttagt gattttagtt gtaacactga ttgggtgcgg attgggggtg
     3301 tggatcaaac atgccttctt ttgagtttac attgctgact attttaggtt gtactttagc
     3361 aacttggctt tctaggatcc taccatttgt cttactgaaa aaattcgatt tgccgcaacc
     3421 gttgcttgag tatttaagtt ttgtcccgat cgtcattatg tctgctttat ggttcagcag
     3481 tctatttacc caaaatatcg gacatctgcc gcagatcaat ctcgaaaatg ctttggcttc
     3541 agttcctaca cttttagctg ctattctttc aaaaagctta ttggtgatcg tcttggtagg
     3601 aattctttcc ctttcgttga ttcgcttatt ataaataaaa aaacaagagc ttatggcaaa
     3661 ttactgtcat gcgctcttgt ttttatcaaa gtcaaataag aaaaaaatcg gcgtattctt
     3721 gcttatccgt tgaacggacc actaacaacg acttttccta acatatgtcc tgtttctacg
     3781 tctttggttg cttgtttcaa gttcttcgca ttcatcccat ctgaatagac cttgctcaat
     3841 gtactgcgga taatcccttc atttgctaaa gcagcaatgt gcgctaaagc ttctccttgc
     3901 gtttcgattt catagttata gtctgtttta gcgaacatat actcccagtc gaagctgata
     3961 gattggtttt tccaaacacc tatatctaac ggctctttga tccctacgat cgttcccaca
     4021 tgtccaaatg gtcggacaag gtgtttgatt tgatcaaagt agcgggtaat atcgaataat
     4081 acagcgacat actctacttg atcgattcct aattcacgaa aacttttact taagtcgtga
     4141 tgataatcga ttggatagtc tacacctgtt tttttcagcc attcaaaatt cttcggactt
     4201 gcagtcgcgt agacttctag gccagcccaa tgcgctagtt gattcaaaat cgaaccgacg
     4261 cccccagctc cgttgatcac taagattttt ttcccgcggt tggcattttc ttctggagtc
     4321 agaccaaatt tttcaaataa caattcataa gcggtcaatg aggtcaacgg caaggctgcg
     4381 gcttcttcat ctgaaagatt ttttggtgca agagcgacaa tacgttcgtc aaccagttgg
     4441 tattcttgat tgcttcctgc tcttgtcgtt gtacctgcgt aaaaaacgcg atcgccgaca
     4501 gaaaattttt gcacttcatc acctacagca acaactttcc cgactccatc aaaccctaag
     4561 atacgtaaag catttcttat cccgttatct tggcgaagtt tcgtatcgac aggattaaca
     4621 gatactgcgt tgacttttac cagaagatcc cgtgaactgg gtacaggtat ctgttctttt
     4681 tcatcaataa agctttttgg atcatcgatc gacaaccctt cataaaaacc gactaccttc
     4741 attaattcct tttctgacat acgaattcct ccttagcttc atcaagttgg agcatccata
     4801 cagttttcat ttcaatatat atagtatgct atttgttcca cttttattca tcataattat
     4861 tctcttactt aaagtaaact atttgcactt ttttggttaa aaaagaggtt gcgacaagac
     4921 ttttgtcaca acctctttta aacggtgttc aatcaactga ccctcggtat tcgctcaaaa
     4981 aacgacaaaa tatgaggagc tatttttgtt tggctgttct tattacttga tgcaggacag
     5041 ctttttcacg acctctaatt cacggtgttc aagcaagaaa tcattgttca ctgaaaagcg
     5101 ttggatgttc tttttcaaag gtgtctaaga tctctcgtac ttcatcagta gatttcgtat
     5161 tcatcaactg aactcgcaag tcatttgctc ctggaaatcc tttgatatag attttaaaga
     5221 aacgatgtag tcctgtgatg gaacgcggga tgatttctga ataatgatct tgcagatcta
     5281 gttgcagacg aagtagtccg atcagttctt tttctgtatg ttccctcggt tctttttcaa
     5341 atgcataagg attcttgaaa atcccccggc caatcatgat gccatctacg ccatattttt
     5401 cggccaattc ttctcctgtc tggcgatctg ggatatctcc gttgatcgtg atcagtgttt
     5461 gcggagcgat ttggtcacgt aaagcaatga ttttagggat gacttcccaa tgtgcatcta
     5521 ctttactcat ctctttacgc gtacgcaaat ggacagaaag attggctata tcttgatgaa
     5581 gtaggtgact gatccattct tccatctcag acatttccgt atagccgata cgcgtcttga
     5641 cgctgacagg caaaccgcca gctttagccg cttcaatcaa ttgtgccgct acttctgggc
     5701 gaaggatcaa gccactgcct tttccacgtt ctgctacatt agggactggg cagcccatgt
     5761 tgatatcgat tccttggaag cccatttctg ccatagcaat gctcatttct ctaaaaaatt
     5821 ctggcttgtc tccccaaata tgggcaacca tcggctgttc atcttctgta aaggtcaatc
     5881 ttcctcgtac actgtctttt ccatctggat gacagaaact gtctgagttt gtaaactcag
     5941 taaagaagac atccggtgct gctgcatgct ttacgacatg gcgaaataca acatctgtta
     6001 catcttccat tggtgctaaa acaaaaaaag gctttggcag ctctttccaa aaattagtag
     6061 tcatttattt gttactctcc attctatcaa gcagtgaagt cgcaatcttt ctcaaaaaca
     6121 ttgtcattat actaggaaac aaaaaaagaa gcaaagtgaa cagcttaaaa gatgtttctg
     6181 attgagcaaa aaaagactgt gacaaaaatc ttatcacaat tccttttccg gaataaatgg
     6241 tgttcaaaag cttgcatctg cggtaataag tccaaccaaa cgaaaaatag gaggagctat
     6301 ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc tctccactaa
     6361 acggtgttca atcagctgac cctcggtatt agctcaacaa acgacaaaat atgaggaact
     6421 attttcccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttttct
     6481 aacttctcaa tcctcttcct ttattgatca aataccaagt aacggcgtac aaaacaacga
     6541 tgaatagcac aagtacgatc atcgatgttg ctactggcac atcgattgtt ccaaggaatc
     6601 cgtaacggaa accggagatc atataaacga tcggattgac tttcgaaaca gcttgccaaa
     6661 atggcggcaa catagagatg gcatagaaaa cgccacctag ataagttaac ggctgcaaga
     6721 caaatgtagg cacgatcgat acatcatcat aagactgagc aaaaatcccg ttgagcagtc
     6781 ctgctaacga aaacaagatt gccgtcatca ataatgtaat gatgaccatg aaccatgaat
     6841 acacatgaag tggtacaaag aacaaggaaa tgatcgttac gagagagccg acgagaatac
     6901 tgcgacccac tccgccaatc acaaagcccc aaatgatcac atgcgtagga acaggcgcta
     6961 ccaagatttc ttcgatgttt ttctggaatt tttgagagaa gaaggacgaa gaaacgttgg
     7021 catacgaact agtgatagca gacatcataa tcaaaccagg cacgatgaat tccatatatg
     7081 aaaaaccacc catgtcaccg atccgaccac cgatcatttt tccgaaaata acaaagtaca
     7141 aggatgtcgt gatcacaggc ggaaccagtg tttggaccca gatacgcaaa tagcggttcg
     7201 tttccttgat agctaaactt tttaatgcag taaaataaag attaaacatt tttctcctcc
     7261 gtatgattat tttcttctgt gattttcaaa aacagttctt ctaaacgatt cgacttattc
     7321 ctcatagaaa gaactttgat tccttgtctt gtcagctgat cgaagattcc gttcacccct
     7381 tgatttcgtt caacttcgac agcgattgtc tgttcatctt caaatgtaca agcgtatcct
     7441 tcaatcacag gtttggtctc atatggcgct aaatcaaaga taaatgtttc aaattgcagt
     7501 ttggatagaa gctctttcat actagtattc tcgatcagtt cacctgattg gatgatcccg
     7561 atgttgcgac aaagcatttc agcttcttct aaataatgcg tcgtcaaaat gatcgttgta
     7621 ccattttcgt tcaattctcg gagaaactcc cacatctcgc gtctcaattc aatatcgact
     7681 cctgctgttg gttcatccaa gatcagcaaa cgtggttcat gcatcaatgc cctagcaatc
     7741 atcagtcttc gcttcattcc cccggaaagc atacgtgcac ggacatttct cttttcccaa
     7801 agattggact gcttcaaata tttttcgctg cgtttcatcg cttctttgcg agagacaccg
     7861 tagtagcctg cttgattcac gacgatttgc tggaccgttt caaagggatt gaaattgaac
     7921 tcttgcggga ctaatccaat ctgttgcttt gctcggacta gatccgtatc cagatcataa
     7981 ccgaagacat tcacttttcc agatgtttta ttgactaaag aagtaatgat tccgatcgtt
     8041 gtagacttcc cagctccatt tggtcctaat agcgcataga aatctccttc tttcacggaa
     8101 agatcgattc ctcgcaatgc ttcgacccca cttccataaa cctttttcaa attatttatt
     8161 tcaagcgcat ttgccattta tttcctatct ccttatagac atgattcttt attgtatcat
     8221 ggaatacagc agatgaaaaa acaacttggt tccagcgaaa aatcaggttg ctgggctcag
     8281 gaaaatctcc taatgaaaga catcaatcgg acgaagtcag acaacctttt cagaaaaaac
     8341 aaatcgaaga taaaggatta ctattcatat gtgcaacaaa aggagcgtga gacaaaagta
     8401 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaaa agccaagatt
     8461 tttgatccaa taaatacttt cgatcacttc gcctatccca atacatgcca ctgccgcttt
     8521 tcatcaaata acgcggcttc cgtttttgcg gtttctacta aagtctctgg gtaatccaaa
     8581 actttcagca aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa tttgaaatca
     8641 aaactgattc cttttccttc tgttacttgt tcttcaaagt ggacattctc acaataattc
     8701 tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga tggatactcc
     8761 gcaagccacc gaacgactga agcggaagaa gcaatccgtt cgatcgtatt tgtccctttt
     8821 aaaatctcat ctacgaagca gtagcagcgt tctttggaag ccacttggtc caacaaacgt
     8881 ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt caaataaatc atcttcgatt
     8941 gccatggaag tcaacacatg tccaggttgc agggtaaact gttctgcaat cgctgtttgg
     9001 atcgtctgtg ctagaataca gctgatggct atacttttga catacgtaga ttttcctgat
     9061 gcattggaac ccgtgactaa tgtattcttc tgccagttga ccggattcac taccgcttct
     9121 tttaacaatg gatgatagat atctttcgct gtcacaccgc cctcagtaaa ctctggtcgg
     9181 caagtgatgg gcatataagt acggtaattc aatattgcag cggctacttc taattctccc
     9241 agcaattccc acagcttgat ggcttcagtt gaatgctttt ctaatttagt gagaacgaaa
     9301 tgataggaga taaatggcag catgaacatg atgttgacat attcaaaaat caactctgct
     9361 tcactcccac tcttcatacg aaatgaaaaa cctaatttag ggatgtctct taatggttta
     9421 gtttgctgtt tcagctcttc ttgaagtggt gtcggcagtt ttgctagctg attggcggct
     9481 gaaatcgttg aaaccaaata actcatgcta ttcagctctg tttctagtgt ttgtttcttc
     9541 actagataat agaccgtatt gaatactaac ccaccgattg ccaaatagat tcctagtact
     9601 tgtcctaaaa gaagcaaacc caaacccacc agcggaagca agccaagaaa aataaataaa
     9661 cctacgcgtc caatcttttt actttttcca tccgctagat attgttttgc aaaattatga
     9721 tctttttttc ctaaacgagc gaattgatat tggatttttt ctctcagttg aggattttcc
     9781 tgataaaaag caatcagttt ttctaactgc tgatcttcac caaaatcaaa gctgcgtaaa
     9841 cgttgataca acgcttctga acctacacta gaataggtac tgttgattcc ttcgaatact
     9901 tcgaacatat ccaggtcata ccaagtaaga tcatcgattt cgctatccca tgaacggaac
     9961 ttcttctccg tgagccatgc ttccttcaag ctttcttctt tatcaaatct cggttgataa
    10021 ggaatttttt cccatttgct acgaactgtt tcctttaatt tcaaacgtga aaaaatgtct
    10081 aaagcaacta ataaaataac agctgcaata ataattaaaa cgataaattc tctttgaccc
    10141 atatccctct ccgttccttt atttgttagc tt
//
LOCUS       DAZKCY010000089         9995 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000089 DAZKCY010000000
VERSION     DAZKCY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.090"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..683)
                     /locus_tag="ACG8XC_002057"
     CDS             complement(459..683)
                     /locus_tag="ACG8XC_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191740.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            874..3651
                     /locus_tag="ACG8XC_002058"
     CDS             874..3651
                     /locus_tag="ACG8XC_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9191741.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3732..4151
                     /locus_tag="ACG8XC_002059"
     CDS             3732..4151
                     /locus_tag="ACG8XC_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9191742.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4163..4633
                     /locus_tag="ACG8XC_002060"
     CDS             4163..4633
                     /locus_tag="ACG8XC_002060"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9191743.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4650..5429
                     /locus_tag="ACG8XC_002061"
     CDS             4650..5429
                     /locus_tag="ACG8XC_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9191744.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5416..6237
                     /locus_tag="ACG8XC_002062"
     CDS             5416..6237
                     /locus_tag="ACG8XC_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9191745.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6254..7303
                     /locus_tag="ACG8XC_002063"
     CDS             6254..7303
                     /locus_tag="ACG8XC_002063"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9191746.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7316..8320
                     /locus_tag="ACG8XC_002064"
     CDS             7316..8320
                     /locus_tag="ACG8XC_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9191747.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8953..9972
                     /locus_tag="ACG8XC_002065"
     CDS             8953..9972
                     /locus_tag="ACG8XC_002065"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9191748.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3358 a         1647 c         2091 g         2899 t
ORIGIN      
        1 acgtaatttt tgtaaaaaag tgggttgaga gaaatctaat tgattttcaa agaaggagta
       61 tgaaaatgag aaaaatattt attcgtttat tgtgggtact atcttagagg aataaataat
      121 agtggagaaa actatggttt ggcgcagatt ctatcaggtt tgatggaacg aagacaacct
      181 cccgacatca agtcaaaagc tacttgagat tttgatctga aaataaaaaa agaaaacaag
      241 gtaggaacat caatgtttct accttgtttt cgtaatcaat gggaaaaaac taagagtggt
      301 gtacgtaatc aataaaaagg atggtgacaa cagtacctct ttactctctg aaagcatcat
      361 aactcgtttt tcctcattat gccaataaat tgttgttata ccacatataa ggatttgtaa
      421 tagctaaaaa cagacaggaa agttgaaatc tgtgaatctt attttgtatg gaaggattta
      481 tagaacgcaa aaatcaagat aaagatgaat aaaaatcctg tttcggctat atattgcgta
      541 tctacgccaa taaaacgagt ttgttctgtc atagccatat aaagcaaagg caaaaataca
      601 actacagcga gataagatcc gatgatttct cgaaaagttt tccaggaagt atgtttcctg
      661 tttttatatt tatccgattt cattgaatag tcatagcctc cttattcttt catatgttga
      721 aattccagta taaaaactgg aaggaactgt acgtttttta gaataaaaat tataagggat
      781 aagtaggctg ctgtctataa taagatatat ttttcaattc tttattaaag attttattga
      841 caaaatgaat ccgttttcta tgatggagga ggggtgaata agttgttgaa gaaggaaatt
      901 ttatgttact tagagaacca gacagccttt ttcgatttag aacatatgaa cgaggtcttt
      961 acagcacaaa ggttagctga tcattttggg gtaaaaagaa acacagtcag tcattatttg
     1021 aaccagttaa atgatgagca actactagtc aaaatcaatt ctcgaccagt gatttatttc
     1081 cacaaagaag cttttgaaaa acaatttttt aagttacgca cgaattttta tttttctatc
     1141 gatgatctca aagaggaaca gccttttttc gcgcaaccaa aagacttatt ctcttttatg
     1201 atcggatacg acgccagttt gaaagaaagt atcgaacaaa tcaaaacagc tctttactat
     1261 ccagatggcg gattaccact tttgattaca ggcgaaagtg gcacaggtaa aagttattta
     1321 gttaatttgg tctatcagta ttgtctgctt catgatctgc tagaagattc tgcaccattc
     1381 atcactttta attgtgcaca atatgcagat aatcctgaac ttttgactag caatttgttt
     1441 ggacatgtta agggagcgta cactggtgca gaagaaaata aaaaaggggc ttttgaagca
     1501 gcagatggtg ggatcttgtt tttagacgag gtacatcgct taacaccaga aggtcaggaa
     1561 aaactgttca cctatcttga tcaaggtgtg atttatcgta tgggagaacc taatttgtca
     1621 cgccggatca aaacacgtct cttttttgct acgactgaag agattactag tcatttctta
     1681 acaactttca ttcgaagaat acctatccga atcgaactac ctgcacttaa tcagcgtagt
     1741 cgtaatgaac ggctagaact gatttactct ttttttctcg aagaacaaag aaaaatgggg
     1801 agccctttgt cagtcagtgg accagctttg tcacttttaa caagtcagaa attatccgga
     1861 aacataggag agctgaaaaa tatcgtcaaa gtttcagcag caaaagcata tgctgaacag
     1921 cgtgaacagc ctgaaatcca tgtcacgatc catcatttgc aaaaagaact acttgcgcag
     1981 cctattacta aaaatacagc acagcaagga atctatatta cgcaagacca gaccttagag
     2041 caattaatgg agaagcaaca gcccgaacag cagcgaatcg ttaaaagttt tgagcaaatc
     2101 ctaattgatt tcaagaaaaa taactgtctg ctatattctt gcgaaggaaa attaaagcaa
     2161 gaagtgattc aattatttga ttttttgctg tttgagacca gtagacaaga aaaacacgag
     2221 ctgctggttt attggacaca atctatccga gagacctttc gacaaatgga aactgcctat
     2281 caaatcaaat tcgatggaaa cagtgtctat gccttgagtt actatctcta tcagcgaagc
     2341 actatcaaat ggttaccgga agaacatgat attatccaac taatcaaaga attagaacaa
     2401 caagtttctc agtcttatcc atctagttat cactatgtcc aacgaattct ggaattaagt
     2461 aagatcaaat tagatattga agacacaagt atggatcgga ttttactgac gatctatcta
     2521 aaaaaagcga aatggtcaaa agaaaaacga ctgccaaaag cgatgattgc tgctcacggt
     2581 tatgcaactg ctagcagtat ggcaaatgta gtaaatcgtt tattacaaga ggatctattt
     2641 gaatcatttg atatgccact agatgtcaca ccacagcaga ttgcttcaga aatccttgat
     2701 tattgcgaaa acagcgatgt ttcaaatgga ttgatcatat tagtagacat gggatctctg
     2761 aaagaaatcc atcagttttt taaaaaacag ttatccgtac cgcttttgat tttgaataat
     2821 gtgacgacac ctttagcaat cacagtaggg gaatgcctgc aaaaaaatgt atcattagaa
     2881 gagatagcag aagagactgt tcgacagatc cagccagaat ggcgactgct ctatccagag
     2941 gaaaacaaac ccaaagcact cattactact tgcttcactg gaatcggtac ggcggtccac
     3001 ttaagtgaac tgctagaaaa aagcttacct acggcttgtc agttgaaaat catcccatat
     3061 gaatatcagc aattaaaaga taagaaaaac agtgacccat tattttccat atacgaagtc
     3121 gtaggaatga ttggcaccac agatcctgat atcaccaata tcgactatct ttcattagaa
     3181 gacctcatct cgggggaaaa gatgtctgta ctagctgaat ggttggacac tactatgaat
     3241 gcttcagaga aggaaatatt caatcagcgc atcattcgta atttttcatt ggaaaaagta
     3301 ctggattccg tgactattct agatacagag aaggtgatgg gagaaatcga tcattttatg
     3361 agagaattag aggtgcagtt gaatcgaagc atcagcaatc ccaaaaaatt agcgttgtat
     3421 gtccatgtga gctgtctcat tgaacgattg atccgacaaa tgccaatcga aacctatcaa
     3481 ggattagaga aatggaagca gtgtcaaaaa gaagggttat ctgctattaa atctgcgttt
     3541 agtgtcattg aagagcatta tagtgtcatg atccccaatt ctgagattgc ctatatctat
     3601 gatattttga gtgaagatac agagtttctc tcgatagaag aagaattttg aacataagca
     3661 agttgttgcc actaattttg gcatgcaact tgctttttta gtatgtgtaa gtgaaaagag
     3721 gagatgaaaa agtggataga gaaattatct tagcttccca tggaaaattt gcttcaggga
     3781 tattagattc attagagctg atctatggga aaaatcattc tattactgta ttcgattgct
     3841 atactgatga gaattttgat ttagcggaaa cagtaaggca gctttttatg aggtacaaag
     3901 ataaagaaat cattgttctg acggatcttt ttggcggaag tgtcaacaac gaattcttac
     3961 aatatatcaa tcgggatcat gtctatctcg ttgccggatt gaatttgcca ctattgattg
     4021 agctagtatc tcggttggac gctgaagtag agacaactgt tttgatccag caggtgctaa
     4081 ctgattcaaa aaaaatgatc cagttttgta acgagcggct caatcagaaa atagaagaag
     4141 acgaatttta ggaggaagaa aaatgattgt attgacaaga gtcgaccatc ggttattaca
     4201 cggacaagta gctttttcat ggacacaaac aataggcgca gactgtattt tgattgctaa
     4261 tgatgatgta cctacgaatg agatacgaaa gacaacgatc aaattagcca agccacaagg
     4321 agtcaaacta gtgatcaaat caattgatga ctcgattgcc gcgttgaaaa gcggggtgac
     4381 agataaatat aagttattca tcgtagtgga atcgatcgag gatgcatata agctggcaga
     4441 agcgtatcca gaaatcaaaa ccatcaactt aggagggatc aaggcaaaac ctggtactcg
     4501 cagcatcgga aaagcggtaa atattttacc agaagaggag gaacgcttga aacgattaat
     4561 tgacaaacaa atagaagtag aaatccgtca agtaccagca gataaaaaag tggatgtaag
     4621 agaagttttg taagaaagga agaacctcaa tgattgtaca agcagtctta ttaggaattg
     4681 ttgcatttgt cgcacaatca gaatatgcac tagggacatc gttgctttct cgtccgattg
     4741 tgacggggct gtttacagga atcgtgttag gtgatgtaaa aactgggatc attatgggcg
     4801 caactttaga gttagcattt atcggatcgt tctcagtagg tgcttcgatt ccacctgatg
     4861 ttgtgaccgg tgggatttta gggacggctt ttgctattac agctggagcc ggtacagaaa
     4921 ccgcgctttt actaggtttg ccgattgcta cgttgacatt gatattgaaa aatatttact
     4981 tagggctttt gattccaatt atgaaccata aagcagacaa ttatgcagaa gaaggaaatt
     5041 ataaaggagt cgagcatatg cacttgcttg ccggtatcgg tctttccttg atgcttggtg
     5101 ttgtggtaat ggtttcttat atggtgggaa gcaatacgat cggaaatcta ttaaatatga
     5161 ttcctgactt cgttcaaaag gggttgtcag tagctacagg gttgattcca gctcttggtt
     5221 ttgcgatgct tgctcgtttg ctgatcaaca aacaagttgc accatatttt ttcttaggtt
     5281 ttgctatagc ttcttactta gagattcctg tgaccggagt agctattttt ggtgcgattt
     5341 tagcagttgt ggtagtcaat attatgaatg tccgtcaact accacttcaa acaactgcag
     5401 gggaggtaga agaagatgaa gaattctgag atcaaaaaca gcaaaataac gaaaaaagag
     5461 ctaaatcacg tattttggcg ttcttttcaa atggagtttt cttggaatta tgaacgtcag
     5521 atgaatatgg cttatgttta tgcaatgatt ccgattttga aaaagttata tcagtcgaaa
     5581 gaagaaatgg cagcagcttt gaaacggcat ctagagtttt ttaacacgac cccacatatc
     5641 gttacactga ttttaggaat cacggctgca atggaggaag aaaataagga tgatcctgaa
     5701 tttgatgtga ctgcgatcga tagtatcaaa acatctttga tgggacctct tgcagggtta
     5761 ggagattctt tcttttgggg aactttgcga ttgattgcca caggtgtggg tacctcttta
     5821 gccatgcaag gaaatatttt aggccctatc ttgttcattt tgattttcaa tattccgcac
     5881 attctgtttc gctatctagc aacaggttgg ggatatcgct taggaacggg ttttttgaaa
     5941 aagatccagg caaacggtat gatggaaagt ttgacattag gcgcgtctat tattggactg
     6001 atggtcgttg gagcaatgac tgccacgatg atcaccatca atattccttt aaaaatcggt
     6061 tcaggagaaa atgctgtgac tgttcaaagt atttttgatg atatcgttcc aaatatctta
     6121 tgtcttggag catttggcat agtgttttat ctattgaaga aagaagtgaa accattaacg
     6181 atcttgatcg gactagcttt ctttggaatt tttggttcgt ggatagggat tttttaaaaa
     6241 aggagcagag aaaatgttta cgatgcaaga ttatatctat gaagaaaagg aagtactaag
     6301 tacgatcttg aaaaagaatg atttttcgac aagagaacat atgaaaaaaa cagtgaattt
     6361 actgatctta gcaacgggtt catcttataa cgcttgttta gcagcgaagc cagcattgga
     6421 atcttatggt gatcttactg tcgatattca agaacctttt tattttttca attatggaaa
     6481 actttctcct tcaatcgata ctgtgattgc tgtgtcacaa agcgggaaaa gtgcttctac
     6541 tgttgaagca gtaaaaatga tacaaaaaca agggttacct gttgttgcta tcacaaatga
     6601 cgtgcaaagt ccacttgctt tagaagctgc acaaattatt gatttaggtg ccggggtaga
     6661 atcagtaggc ttcgttacaa aaggctattc tgcgactgtt ttacagttac tactcttagg
     6721 gataggaatt ggaataagta aaaataaaat cagcaaaaaa atagaacaag actacatgca
     6781 acagttgcgc aaaataatca accatttacc agcgattatt caaaaaacgg aagaattttt
     6841 tgatggatat caaagtttat ttcgtttagc acagcgtttc gtcaccattg gatatggtcc
     6901 aaactgggga acagctaaag aagcagagac gaaattgact gaaacaatcc gcgttccgtc
     6961 acaaggattt gagttagaag cttatatgca cggcccatac ctggaggcag atgcttcgca
     7021 tttgctattc tttattgaag gggaaagtgt aaataaggaa cgttcgcaaa agttgcaaag
     7081 atatatgagt cggtacgttg gagagactct caccattacc acaaaaaaag caagaaacga
     7141 gaaaacactt ggattagcaa tagaatgtga tgaatatctg tctgtattag cgcttgtcgt
     7201 tccgtttcaa ctattcgctt ataaaaccgc agtagctaaa ggtattgact tgaacaaaaa
     7261 gatttttgaa gactttgata cagtcttgaa aagtaaatta taagaggtgg aaatcatgct
     7321 gaaattcaat gaagaagaac aaatcaaagc aataaaaggc gcactagctt tacgtccgca
     7381 agtagaagaa atcatcgata aactttatgc ggaaaaattt gacgctgtct attatctagg
     7441 aatcggtgga acgtatgctt cctcgatgca agctgtcact tatatgaatg ggaaaagcaa
     7501 cttgcctgtc tttgtacagc atgctgcaga atattacact actggaaaca aaagattaac
     7561 aaaagattct atcgttattt tgtcctctgt taccggtaca actcaagaag tagtgaaagc
     7621 agtagaacaa atcaagaaag taggagcgac actgattgga tttatcgata aagcgaacag
     7681 taagttgtct cagctatgcg attttgtggt cacttatcca gctcctggaa cagaacaaat
     7741 caaatttttt atggcagcag atcgattgat gtatttgaat ggagaattcg aagcttattc
     7801 agaatactat gaacagctcg aacgttattt accaacaggg ttagtggaag cagagaaaaa
     7861 agcagatgca tttggcttat catttgcaga aaaacatcga cgtgatagta tgcattattt
     7921 tattggagca gggaatcaat ggggagctgt ttattcatat gccatgtgtt actgggaaga
     7981 acaaagctgg cttccatcga aatctatcca tgctgccgag tttcttcacg ggacgttaga
     8041 gatcgtagaa gaaacaaccc cagtcacatt atttttaggc gaagatgaac agagagtgtt
     8101 atctgaacgt gtggctaaac ttttgccaaa aatttgcagc aattatacat tgatagacac
     8161 aaaagactat ccagtagaag gtatcagtga aaaatatcgc ggacgtgttt tgtctttctt
     8221 attgatgcat gtagtgacac aacgcatcga tgctcatgtg gagcagttga actgccatcc
     8281 attagagata cgccgctact atcgtcagtt tgattattag gatagactgg ataaaaagaa
     8341 tataaaaaat aaagagagca gcgataactg ctccctttat ttttttaaaa tattggcatc
     8401 aattacgtta taaaacacat tttttgcatt atctacagtt caacttataa tctgtttaat
     8461 gggacatgtg ggtgccaacc attttttgtt atgcaatagt actatttagt tgttgcatgg
     8521 atccgtagta aaagtccaag tacaacgagt tgcgccagtt tacatagcgt tttgtatcca
     8581 gaatgttgac taaaacaaaa gttcctatgt caaccgtcaa cagagacaat ctgtccgttg
     8641 caggacctga cagatttaga aatgttcaat ctgtctctta tgtattttaa atcaatactc
     8701 actaggagaa gtttcctaac ctcctaatag taatgctggt agtaacattg tcaaaactgc
     8761 ttcctcttag gaacaagcaa agcataacga gtgtaaaaat aaaatgtaga ctattgtaac
     8821 atatcattga ttatttcaag taaaaacgat aaaagaagta aacaaacact taaaaacaat
     8881 ggaaattagt cccaacttta agaaatgtat actataatgg aaattatcaa ttatttattt
     8941 ggaggaatat ccatgaaaca aaaaatcgca gttcttggtc ctggatcttg gggcactgct
     9001 ttagcacaaa cattggcaga aaacgggcat gatgtccgta tttggggaaa tgtacctgaa
     9061 cagattgatg aaatcaataa gtatcataca aatcagcatt ttttacctga tttgacgatc
     9121 ccagaatcga ttatcggata taaagaattg gcagaagcag tggataacgc agatgctatt
     9181 ttatttgttg ttccaacaaa agcgattcgt tcagtcgcac aggaacttat tggtaaaatg
     9241 aatactaagc caatcattat acatgcaagt aaaggactag aacaagatac gcataaacga
     9301 atctcagaag ttttggctga agaaattcca gaagaaaaaa gacaggcaat tgttgtatta
     9361 tctggtccta gccatgctga ggaagtggca gtacatgata tcacaacaat tacagctgca
     9421 agtattgatc aaaaagcagc agcctatgta caaaaactgt tcatgaatga ttatttcagg
     9481 atttatacaa ataatgatgt gattggcgta gaaactggtg cagcactgaa aaatatcatt
     9541 gcaattggtg ccggcgctat ccatggatta ggattcggag atgatgcaaa agcagcaatc
     9601 atgactcgag gattggcaga aatcagccgt cttggtgtcg cgatgggtgc caatcctctg
     9661 acatttatcg gtttgagtgg tgtgggggac ttgatcgtta cttgtacaag tgttcattct
     9721 cgcaattggc gtgccggtaa cttgttagga cagggacata agctggaaga agttctagaa
     9781 aacatgggaa tggtcgttga gggtgtggca acgacaaaag cagcagtaga gctagctcaa
     9841 cagctaggtg ttgagatgcc aattactcaa acaatctata atgttttgta taatggagaa
     9901 gatatcaaaa aagcagcaaa agaaatcatg ttacgtgacg gaaaaatgga gaatgaattt
     9961 tctcttcgtt aaatagatga tcaatagtta atcgt
//
LOCUS       DAZKCY010000090         9985 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000090 DAZKCY010000000
VERSION     DAZKCY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.091"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            283..522
                     /locus_tag="ACG8XC_002066"
     CDS             283..522
                     /locus_tag="ACG8XC_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191749.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="ACG8XC_002067"
     CDS             complement(884..1144)
                     /locus_tag="ACG8XC_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191750.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="ACG8XC_002068"
     CDS             complement(1329..1826)
                     /locus_tag="ACG8XC_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFP9191751.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ACG8XC_002069"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ACG8XC_002069"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFP9191752.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ACG8XC_002070"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ACG8XC_002070"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFP9191753.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5210)
                     /locus_tag="ACG8XC_002071"
     CDS             complement(4548..5210)
                     /locus_tag="ACG8XC_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9191754.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5220..6035)
                     /locus_tag="ACG8XC_002072"
     CDS             complement(5220..6035)
                     /locus_tag="ACG8XC_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9191755.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6100..6996)
                     /locus_tag="ACG8XC_002073"
     CDS             complement(6100..6996)
                     /locus_tag="ACG8XC_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9191756.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            complement(7049..8308)
                     /locus_tag="ACG8XC_002074"
                     /pseudo
     CDS             complement(7049..8308)
                     /locus_tag="ACG8XC_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(8337..8650)
                     /locus_tag="ACG8XC_002075"
                     /pseudo
     CDS             complement(8337..8650)
                     /locus_tag="ACG8XC_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            8707..8853
                     /locus_tag="ACG8XC_002076"
                     /pseudo
     CDS             8707..8853
                     /locus_tag="ACG8XC_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(8941..9234)
                     /locus_tag="ACG8XC_002077"
     CDS             complement(8941..9234)
                     /locus_tag="ACG8XC_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFP9191757.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKNILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            9271..9456
                     /locus_tag="ACG8XC_002078"
                     /pseudo
     CDS             9271..9456
                     /locus_tag="ACG8XC_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9736..9966
                     /locus_tag="ACG8XC_002079"
     CDS             9736..9966
                     /locus_tag="ACG8XC_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191758.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         3119 a         2051 c         1532 g         3283 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattttgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaattg caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 ttttcaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattaccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcttt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcagc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aagtgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagc agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa
     5041 agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct
     5101 gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac
     5161 caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc
     5221 tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat
     5281 ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca
     5341 tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac
     5401 aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa
     5461 ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagatttc ccactctgat
     5521 aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta atattgcttc
     5581 aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatct
     5641 gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca
     5701 gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa
     5761 aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa
     5821 aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc
     5881 tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct
     5941 ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc
     6001 agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt
     6061 tttctttcat ttcgaacact ctcttccata tcacacctcc taagtacgac gcttattcca
     6121 agaaaattga acaaccgtaa cgcccaaaac aattacgaac aaaacaagtg aaatagctga
     6181 agcataacca aaatcaaatt ttttgaatgc ttcattgtag atttggtaaa ccagcacatt
     6241 tgtcgctcga ccagggccgc catccgtcat cacttgcaca aggtcaaaca ccttgaaaca
     6301 attgatgaca agcatgatcg ttacaaagaa ggttgttggt ctcaacgaag gtaatgtcac
     6361 attcaaaaat tgtttccaac ggtttgctcc atctagcgaa gacgcttcat atagttcttt
     6421 ttggatactt tgtagtcctg caaggtatag aatcatgtaa taacccatcc ctcgccaaat
     6481 actgacaatt acaattgccc aaagcgccca atttgaactt gaagtccaac ctggaggatg
     6541 ttcaataaat cgtcttaaaa agatattaat aggtcccact gttggatgaa acaacatatt
     6601 ccaaacaaca gcaattgcta ctaaggaagt aacatacgga aagaaaaata tcgtacgaaa
     6661 aagcttcatc cctctaattt tctgatttag tagtaaagct agccctaacg aacaaacaag
     6721 tgtgccaggc actacagcag cggtatataa aaaagtattt ttcaaagaaa tccaaaatgt
     6781 ctcatcgttg gccattttac gaaagttatc tagtccagca aattctggcg ttgaaaaaga
     6841 atcccatttc ataaatgcta gtacaagcga aaacaggatt ggtattaatg taaaaacgaa
     6901 aaaaccaatg aaattcggag cgataaacga ataagcgatc cacgttcgtt ttctacgcaa
     6961 atgatttgta gctttcttat ttatactatt ctccatttcg atgacgaaac caagagtgtt
     7021 tgatctcttg gcttctctcc tttcatgatt attctttttc cgcagctaca cgtttctcca
     7081 tatttaccgt tcctttttcc ggcgtttcat ctccaaccaa aactaagtca tgttcttctt
     7141 gtaaaatctt atcgattgca ggcccatacg tatcaatcgg aaattcaagt ttgatttcat
     7201 caggagaaaa agctttttta gaaatttcat ccgttggcat tccagcgaga ttaaaataaa
     7261 gctgatcaat ctcatccgta cgatatgaag gaactactcc tactcctgct aatacttttg
     7321 ctccttcttc actggctgca aattcaataa attcttgcgc cgctttttgt ttctttgagt
     7381 ttttattgac agcaaatgct gttggtgagc caaacgtcgt gaccgattct ccttttttct
     7441 tctgaggaat agctgtaatt ccccattcta cttctgtctt acttgcttct ttgtttgcga
     7501 gtatgccagc catataccaa gttcccatgt acatcattgc tgctttttgt tcttcaaatt
     7561 gagactgata ggttactttc gttgatttag ccgtgccaaa atccatctgc gcgccttctt
     7621 tttgcatcct caatgctcga tcataataat cagtcatata ttaataatct ggttcaacaa
     7681 gattcgcctc gttttgagca gctgcaatag cttggattgt cgaacgccag atatgttgat
     7741 atgctccgta aacttgtccg tcatttttac taagtttttt tgctaaatct tcatattcgt
     7801 cccaagttag attttttgga tattcgattc ctgcatcatc aaacattttc ttattgtaat
     7861 atagtaccca aaagtccgtt cgatatggca atgcatatgt ttttccatcg atatcataca
     7921 tttcatatgt ttcttcagca gcatcagtgt ctaattctct aattcgttct gtttgatcga
     7981 ctaactgatc tcgtaaagca taatttgaat acgacaaaag atttttcatc gtcaatacat
     8041 ctgtcgtgtc tccagaagaa agcatcgtgg tgagtttcgt atcataatca tctgaagcaa
     8101 tatctactgc tttcactttg attcccgttt tcttttcgaa tgcttcaaag agcgctttga
     8161 attcaggtgt tgtttcataa ttccatgtcg aaacggtaat ttcatttgct gattcttcac
     8221 tactgtcttc ttgattacca caagcaccaa atacgagtga cattcctaaa actattgatc
     8281 caatcatcat ttttttccat cgcttcatta tatgaatcct cctcttctct tatacatcac
     8341 cagtaacagt accatgtttt agctagacga gtgatccctt ctacataaaa gtaatcgccc
     8401 cagatcacac attcatctac accttttaag ctgtttttat cataaacggc atggagaagc
     8461 agaccgtttg atccgttaac tcgtttcgtt gtatagtttt tctgtaattc ttccaagatt
     8521 ttgattgcta tctcttcgta atcgaaatga cgaggatctg aaacaggcag ttgttggaaa
     8581 gttctagcaa tccacatgcc gctatagctg ctgcagaact atctttttcc tctttgctat
     8641 cttctttgaa tctaatttta ggaggtacgt tttggtattt attcgtacaa ataaaagaga
     8701 attatcttta ttcatgtaac gcatccaatt gattttttca tgtacgctcc tggaacgatt
     8761 ttactgacga ttgcttattt aactgcgaat cgctcattag cttttatcat agctatccat
     8821 attttgaata acgttcttgg ctttgtatta tagatacaga tgacaaaaaa agttcaagta
     8881 tgcggtgtag aaccaatatc gcatacttga acttttttca atgagtataa attaaaactt
     8941 ctaattttca attcccatca tctgatatgc ccgaccgtct gaacttcttt tgaaaaagcc
     9001 aaatgtgatt agataacgtc tttgttcgac ataatcgcca ctagaaaata cgttcagcag
     9061 tatcgaatta acctctgttt ctgtataagt cactccatgg tcaaagcgtt tcaagacttc
     9121 tttcaatagg atgtttttct tttttgttga acttggtact tgttttactt ttccatcatt
     9181 catgaatgtg tctaaaactt tcattttttc ttgctccgta taagtataaa ccatcttctt
     9241 tctccttttg attctaagaa tactccattt atgttagaat attctggatt ttttattaaa
     9301 gcggttactc ataagaaaga tattatcata gtgctctttt tttactacta cttataggta
     9361 gatttattct aatccattat tctcccctac caataattaa agaaaaaaac tggatcaggg
     9421 tgttcaaatt acatgctaag aaaaaagaac ccttgaatac caagggttct ttctcagtct
     9481 gataacaatg attaacgacg gatttctttg atacgagcag cttttccgtg taatgcacgt
     9541 aaacaaaacc actatattct attatgtatc taacaccctc aaaacagcta taaatcaaca
     9601 ataccgacat aaaataaaca ctatgttcaa tctgagatat atcaaaggta aacgcatttt
     9661 tgcccccttt ttgcccctca aatatcgctt tacaacaaga acgtaagttc gtataatcgt
     9721 tttgaggtga tttttatgat ggaagaattt attagaaaaa atatcagtga tgaatatgca
     9781 gacttttatg atcaaagcag taaaaaagat aaattccaga tggatgtttc aatcttagct
     9841 atattagctt tttccgaaaa taaacaacct gtaactgcaa aaaaagaaac agtattctct
     9901 gaaggcaaaa taaaaactcg atatatatta gaggtagaaa ctaattttaa aaacagatcg
     9961 gagtaatggt tatgcttttt aatga
//
LOCUS       DAZKCY010000091         9931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000091 DAZKCY010000000
VERSION     DAZKCY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.092"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..3344
                     /gene="fss3"
                     /locus_tag="ACG8XC_002080"
     CDS             117..3344
                     /gene="fss3"
                     /locus_tag="ACG8XC_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFP9191759.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3457..3771
                     /locus_tag="ACG8XC_002081"
     CDS             3457..3771
                     /locus_tag="ACG8XC_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9191760.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3784..4158
                     /locus_tag="ACG8XC_002082"
     CDS             3784..4158
                     /locus_tag="ACG8XC_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9191761.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4168..4503
                     /locus_tag="ACG8XC_002083"
     CDS             4168..4503
                     /locus_tag="ACG8XC_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFP9191762.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4586..5926
                     /locus_tag="ACG8XC_002084"
     CDS             4586..5926
                     /locus_tag="ACG8XC_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9191763.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6004..6678
                     /locus_tag="ACG8XC_002085"
     CDS             6004..6678
                     /locus_tag="ACG8XC_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP9191764.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6671..6835
                     /locus_tag="ACG8XC_002086"
     CDS             6671..6835
                     /locus_tag="ACG8XC_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191765.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6911..8053
                     /gene="dcm"
                     /locus_tag="ACG8XC_002087"
                     /pseudo
     CDS             6911..8053
                     /gene="dcm"
                     /locus_tag="ACG8XC_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            8043..8333
                     /locus_tag="ACG8XC_002088"
     CDS             8043..8333
                     /locus_tag="ACG8XC_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191766.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARMELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            8589..9773
                     /gene="mobT"
                     /locus_tag="ACG8XC_002089"
     CDS             8589..9773
                     /gene="mobT"
                     /locus_tag="ACG8XC_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP9191767.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            9770..9907
                     /locus_tag="ACG8XC_002090"
     CDS             9770..9907
                     /locus_tag="ACG8XC_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFP9191768.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         3282 a         1679 c         2247 g         2723 t
ORIGIN      
        1 attcattctc aaatccattc tattcagtca ataaaaattt taaaaagact ttataaacaa
       61 agatgttcaa gcgagcatct tatttttttg tgcttaattt gaaaggagaa catttcatgt
      121 ataaaaaaca aaaaaagaaa ttatgtgtat taactatttt gagtttggct gttcaagcaa
      181 ttgttggagc aattgctcct acgattactt ttgctgatga aattacccat ccacaaacgg
      241 taaccgtaga actagattta gcacatcaat atgctgttga aggaactttt agtgatggtc
      301 gtccaatgtc agaagtcact gttccgcatt atgcagtgta caacggcttt aaacaagata
      361 ttttctgtat tgaaccagga gtcccgattt acaatgagtt cactcctggc tatgagaaaa
      421 acccgttacc agatatgtcc gataaagcca aattagtttc cgttttatgg aaaaaagtag
      481 gtacagatgc agatactcat attgtggcac agaagatgat ctggcaagaa gttaatgggt
      541 atacccttca ctcaataaaa cgatcagatg gtagtgccgt aaatatcgca gccattgagg
      601 caaaaataaa tcaagccatt gacgattatc agaagaaacc aaccttccat aatagtacgg
      661 ctaaaacagt attagggcaa tcgactactg tgacggatac gaataatctg aatttgtcag
      721 agttcgatga ggttgtggaa aatgccgcaa acattgatta tcgtgtgaat ggtaatcagt
      781 tgataatcac cccaaatgcc aattcaaaag aaagtggtgt gctgacgctt aagaaatctg
      841 ctggtaccgg aacacctgtc gcctataaaa aggctggcca acagacttta atggccgggg
      901 cgattgataa acctaatacc tacacaatca aaattgatgt ggaaacggag ggttctttaa
      961 agatcaaaaa agtcgataaa gaatcgggtg atgttgtacc aggaacggtt ttccatttag
     1021 actttggaaa aactttacct gcaaaagacg tgactactga caaagaaggc attgctacat
     1081 tggatgagat tcctcatggt acaaaagtaa ccattaccga aaaatcagta ccggcgcctt
     1141 atacgattga taccaccccc atgactacca ccattaaagc aggcgaaacc atttacgtga
     1201 cttcaaaaaa cgcacgggaa aaagggcaaa tcattctgga taaaagcgga gttgaaacag
     1261 ggagtgatct ttggaatgac aattattcct tagctggtaa tacgtttgcc attcgcaaag
     1321 acagtccaac cggtgaaatc gttcaagaaa tgaccacgga tgaaaacggt catgcggaaa
     1381 caccaaaaga gattgctaat gcgttggaat tgggaaccta ttatgtgacc gaaactaaag
     1441 ctagtcatgg gttcgtgaat acgttcaaac ctgtaaaagt cgaattgaag tatgccaatc
     1501 aaaccgtagc ccttgttacc agtaacgtaa aagggcaaaa tcaagaagtc actggcgaaa
     1561 ccactttgac gaaagaagac aaagatactg gggataaagc acaaggtaag gcagtctttg
     1621 agggtgctga atacaccttg tttactgcta aggatggaaa agctgttaaa tggagtgagg
     1681 cttttaaacc agagttagcg aagggaacga aaggttctga tgaaacggtc actttgactt
     1741 tagacgaaaa gaaccaagtt gccgttaaac atttagctat caacgagtat tattggcaag
     1801 aaacaaaagc gcccgaagga tatagccttg atgaaacaaa gtatcctgta tccatcaaaa
     1861 aggtggatga tagcgaaaaa aatgccgtga ttacccgaaa tgttacggca aaagaacaag
     1921 tgattcgctt tggctttgat ttctttaaat ttgctggatc agctgcgggc actaccgaaa
     1981 ctggcttcaa tgatctgacc tttaaagtgt cgccattgga agggaccaat gaaatcacag
     2041 gtgccgaaga tgaagcgacc acagcttata acgagcaatt agggtttgat ggttatggca
     2101 agtttgaaaa tcttccttat ggggattatt tacttgaaga agtagaagcc ccagaaggtt
     2161 ttcaaaagat tacgccatta gaaatccgtt ctacttttaa ggaaaacaaa gaagactttg
     2221 tgaagagtga gtatgtcttt accattaccg aacaagacca aaaacaacca attaaaacgg
     2281 tgaccgttcc ttacgagaag ctgacgaaca aagcattttc tgttagtttg aaccgtttaa
     2341 tgctctatga tttgcctgag gaagaagata gtttaacttc tcttgcgact tggaaagacg
     2401 gaaacaaaga attaacttct ctggattcta ccgagctagt tgataaattg agttacaatc
     2461 tacatgaaat caaagaagac tggtatgtgg tagcgcaagc cattgatgtg gatgcgacaa
     2521 aagctgccca agaaaaagat gaaaaagcca aaccagtggt gattgcagaa acaagcgcaa
     2581 cgttggcgaa caaagagaaa acaggaactt ggaaaattca gcataaatta accgccgaac
     2641 aagttttaaa taaaactatt gtcttattca actatgtgta tgaaaataag gaagcttttg
     2701 aagcaggcga taagccagta gcgaaggatg ttagcttgaa taaccaagcc caaactgtca
     2761 gttgtacagt ggaacaccat gtttctatcc aaacaaaggc ccacctagaa aacggttctc
     2821 agacctttac tcatggtgat gtggtggata tgtttgatga tgtgtcgatt actcatgatg
     2881 tactggatgg ctcaaaagaa gctttcgaaa caattctgta tgctttatta ccggatggta
     2941 caaacaaaga aatttggaaa tctggcaaga ttgattatga agtaaatgat aaagaattca
     3001 ccaaaactgt gcttgcgaaa aaagtagata ccggaaaata tccagaagga accaagttta
     3061 cttttgcgga aatcaattac gataaagatg ggaatatcaa tggaaaacac aatgaagatt
     3121 tgaaagaaaa atctcaaacc ttaacaccaa aagaagttcc aaccatacta agtacgccga
     3181 aacaaccgga aacaccaact gttccaagtg gctctcaaga atctagtccc acagtgaaga
     3241 ccttccctca aaccggggag aaaaattcca atgttttact gttcattggt tttactttga
     3301 tttttgcgac ggcgggctat tatttctgga atcgccggaa ctaaggtgat gagatgatac
     3361 aagtaaatcc taagaaccaa cgtgcgcgac cgcctcctaa aaaagtagtc gcgctatttt
     3421 tgtatccaaa actaacaatg aaaagaggaa aagaagatgg aattgaaatt tgtcgtgcca
     3481 gatatggctg aaacctttgg aaagttaagt tatgccggtg aaggagaaat tataacagaa
     3541 ggatatggac ggaatactac ggtgattggt cgcagttatc atttgtattc cagtaagcaa
     3601 cgggccgatg atattgaggt ggtagtagct gcggaagccg gtgaaaagga ttttgatcaa
     3661 gaccaaccgc taaaagccgt gaatccccat ttggttgcca aaggctatga gattgagaat
     3721 cgtgggttta ctgattacgt gttgtatgtt gatgatttag tgaaagcata ggaggaaaag
     3781 aagatgagat taacagaagg tattgtcgta gattcaggtt taacatttgg gaagttgcgt
     3841 ttttccgcat tacgccgaga agtacggaag caaaatgagg atggaacaat tggtaatgag
     3901 gtaaaagaac gaacctataa tttaaaatcc tctgcacaag ggcgaatgat tcaagttagt
     3961 atcccagcta atgtaccctt acgcgagttt gcttatgatg cagaggtaga gttggtcaat
     4021 ccaatcgtgg ataccgttgc caattatgtt tttcgagaag gaactaccgt taattggttc
     4081 attaaggcgg atgatttagt cttgaagcgg cagccgaatc aaggaaatcc tacgaaccaa
     4141 aatgaaagaa agaaataggt gaagattatg gaaattaatg ttgaacatat tttagattgt
     4201 ctcgatcaat atggaaaagg cgagctgact gaggaacaac tcgttaaagc attaacctct
     4261 gatgaaaaaa tgtttctgat tatacatcaa ggtttacttg gagaaaatag cgacagtgaa
     4321 gaagactctg atgtactaga ctggttagga gaagaagagt ctttctttat agttgtcgaa
     4381 gtaaatgaaa acttatgtcg gcaggcagaa tccattttgg aagaaattgg tgtggagatg
     4441 ccggatgcta ttgaaggctt tttaaatcaa ttggttgaaa cgaaacaact cccctcctcg
     4501 tagcagtaaa tgactagcat aactttttga aattgcctaa tgaaatccta ttgggctttt
     4561 tcgattggag gtggaaacca tcaaaatgta caaaggacat cgcatacgcg caggagatca
     4621 gcacttggtt tatcactttg ttcttggctg gctactcgct ctgttcatcg gttggatgag
     4681 cgtcttttat tttcaagagt ttagacaatt tgatatttct aagctatcac tttctactat
     4741 tgaaatcgtt tggtctataa aagatttagt atgtctgctt ggaagtctcg ctttttcagg
     4801 agctatgata ttgctttata ttcacttttt tcttgatcat tggcgaagct tgtggcatcg
     4861 acaaaagcta gctcggatga ttctagagaa ccattggtat gaagtgaaac aaactcaaag
     4921 tgaaggcttt ttcaaagatt tgaatagtag ccgaaccaga gagactatca gttacttccc
     4981 caaaatctat tatcgaatga aagatggatt actgtctatt cgcgttcaaa tttcacttgg
     5041 gaaataccaa gatcagcttt taaaattgga aaagaagtta gaaagcggac tgtattgtga
     5101 gttagtagaa aaggaactca aagactccta tgtggagtat actttactgt atgatatgat
     5161 cgccaatcga attgggatag acgaagtagt ggcagaaaat gggactctgc gattgatgaa
     5221 aaatcaagta tgggcttatg attctttacc ccatatgtta atcgctggtg gcacgggtgg
     5281 agggaagacc tatttcctcc tcaccattat cgaagcacta ttgaagtcag atgcagaatt
     5341 gtttatcctc gatcccaaaa atgcagattt agctgattta ggtacggtga tgcctcatgt
     5401 ttattcacaa aaggaagaaa tttctgcttg tgtggaagat ttctatgaac gtatgatggc
     5461 tcgtagcaag gcaatgaaag aaatgtctaa ctacaaaaca ggagagaatt atgcctatct
     5521 tggacttcca cccaactttt taatctttga tgaatacgtg gcttatatgg agatgttaac
     5581 gacgaaagaa agtgcggtga ttttgaataa gctcaaacaa atcgtgatgt tgggtcgtca
     5641 atctggtttc tttctgattt tggcttgcca aaggccggat gcgaaatatt taggagacgg
     5701 aatccgcgat caattcaact ttcgcgtggc tttgggtcgt atgagtgaac tcggatattc
     5761 catgatgttt ggggaggtcg aaaaaaactt ctttatgaag cacatcaaag gtcgaggtta
     5821 tgtggataca ggtggcagtg tgattagtga gttttatacg ccacttgtac caaaagggta
     5881 tgatttctta gattcaatta gtaaattaga aaagatgaat gattgagtga tcttcaatac
     5941 tgactttata cttcaagtaa cgtataatac ttttatatag taggaagaaa ggggataatt
     6001 aatatggaaa gtaatgattc ttatttagag tatgcaaaag caaataagga taagtttatt
     6061 tcttatatta tcaaagggaa atcccccgaa gttggacgag acgcagtttt tatggcaggg
     6121 agtcctgggg ctggaaaatc agaggttgcc attggattag ctgagaatta tgataaccat
     6181 gtgattattg atgcggatta ctttcgcacc cagtttcctg attataatgg aaaaaactct
     6241 agtctctttc aaaaagcatc ttcttggttg gtagagcagg catttagata tgtcttagat
     6301 catggctatt cttttattct ggatgcaact tttgcaatat taagtgctga aaaaaatatc
     6361 tcgcgggcgt tgaaaaaaca ttttcgagtg acgattttct atgtttatca agaccctaaa
     6421 atcgcttgga aatttacaca agaacgtgaa attgcagagg gacgtcatgt gccgaaaaag
     6481 gcatttatta acgctttttt caaatcaaga gagaacattg caaaagtaaa gagtaaacat
     6541 ccagatgtat tgcttcatat cataataaaa gattatacga ataatatttc cgaagttcat
     6601 tatgctacgg ataatctaga attattttta ccactagggt atagtaggaa agatttggag
     6661 gttcaattac atgactgaga aagaacaagt aacgaaaatt gtaaagaaat acaataagag
     6721 cattgcggat ttatcggaaa atgctactgc aaaagagttc aaaacggtga ttaaatatgt
     6781 agccgatcag gccaatgaaa aacaacggaa attagttgga ttaaataaaa aatagtagga
     6841 caaattaata atgtatgagc caagagcaaa atgttcttgg cttttttctt tgcaatgaat
     6901 gaggtaatgt atgagatttt tagatttatt cgccggtatt ggtggctttc gtttaggtat
     6961 ggaacaggcg agccatcatt gtatcggttt ttgtgaaatt gataaatttg ctagacgtag
     7021 ttataaagca attcatgata caagtaagga ggtggaaatg catgacatca caagtgtatc
     7081 agatgagttt attcaatccc tcggaccagt ggacatcctt tgcggcggat ttccgtgcca
     7141 agctttttca attgcgggaa agcggcaaag attttccgat actcgaggta ctttattctt
     7201 tgaaatcgct cggttcacca ctcttctccg acctaagctt ttattccttg agaacgtccg
     7261 aggattgctc aatcacgaag gaggggctac gttcgagacg atcctccgaa cgctggatgg
     7321 attggggtat gacgtggagt ggtaggtgct taactcaaag gcctacattc cccaaagccg
     7381 ggagcggata ttccttatcg gacattctcg agacgcatgt accgaacaag tatttcctat
     7441 cattggaccg tcttcaacat ctgatcaaaa catcagaaat ttggtaaata tcaatccttc
     7501 caatcgagga atggggggac aagtctatgg ttcagatggc gtagcgccaa ccttaacagg
     7561 tgatgaagga atcaaaatcg cactgccagc aagggatgga atctctgtag caggactatt
     7621 accgggaaag tttgaacaag gtaatcgagt ttatgaagtt actggaaccg cccccacctt
     7681 gtcaacgaaa caaggtggta caaaaatcat gattcgaaaa aatgaaacag aatatcaaga
     7741 ggtgaagcca gaagacagtg tgaatctggc ttttcctaat tccacaagtc gccgaggtcg
     7801 tctgggaaaa cagagtgttc ataccttact aacaggagac caacaagcga tcgtcacgga
     7861 tcagtatcaa attcggaagc taacacctag agagtgttgg cgacttcagg gctttcccga
     7921 ttgggctttt gatcgagatt ctcaagtcaa ttcagatagt cagttgtata agcaagccgg
     7981 aaactctgta acagttccgg tcatttttga tattgccaga agattaaaag agttggatgt
     8041 acatggttta tgatgagttg attggagaaa tttattgggt gattggaaag attcagtcag
     8101 acccagaatt agaagaacaa ttgcgacggt taaaatttga tattcgcaag aatggtgtca
     8161 aagtcgctgg cgatccatat ttaatgaatg aggagacgga tgctcgaatg gagctgaatc
     8221 aagtcatcag tgaattggaa cgaattgcgg actttgctaa agaacctgat atccgccaat
     8281 acttgtttga gataaaagca gagttggaaa ttgagggtat taccgcggag taacggccaa
     8341 ataaaccact gataggcact gaccgcgggg gatttgcgaa gcagaaatcc tccgtggtgc
     8401 tgtgcctgtt tatggacggc gttagccggc catgatgtct caccccccgt atctaacagg
     8461 ggggtacaaa aacacaaaaa ataagcaagc aacgataagg aatggctgtc ctatcaagga
     8521 taataagcaa tttgtgtaga tgttaactga tttgaaaaag ttaacacctt aaatttggag
     8581 gtgagaactt ggcacaaagg aatttagatt atcggctatt aaaagaccgg cgtaatgaat
     8641 atggcgtttc acaaaataaa ttagctacga cttgtggact tagtcgcccg tacttaaatc
     8701 agatagaaaa tggtggcgta accgcatcta taaaaaccat gaggaaaatt tttgaccaac
     8761 tggaaagttt caatcccgag ttacctttaa cgttactgtt tgactatgta aggattcgct
     8821 ttcccacaac ggatgcacga aaaattattc aagaaattct ccatttgaaa tttgattata
     8881 tgctccatga agattatgcc ttttactcct atcaagaaca gtatgtcatg ggtgatattg
     8941 tggtgatgtt gtctcatgaa gaagataaag gcgtcctttt agaattaaag ggtcgtggtt
     9001 gtcggcagtt tgaaactttt ttactcgccc aaaagcgtag ctggtatgac tttttcgaag
     9061 attgtctaaa agccggtggc gtgatgaaac gcttagacct ggcaatcaat gatcgagttg
     9121 gcctgttaga tattcctgat ttaacgaaaa aatgtcagaa ggaagaatgt atctccttgt
     9181 ttcgtacctt caaaagctat cgttccgggg aactgttgaa acctgatgat aaagatggta
     9241 tgggcaatac tttgtatatt ggcagtctta agagtgaggt ctatttctgt ttgtacgaaa
     9301 aagactatga gcaatatatc aagttaggga ttccattaga ccagaccgaa acgaaaaatc
     9361 gttttgaaat ccgactgaaa aatgatcgag cttatcatgc aattcaagat ttgttaaaag
     9421 gtcgcagtat tgaaagtacc acattttcca ttattaaccg gtacttgcga tttgcggata
     9481 aggtggaagg caaaaggcga actaactggc ccttgaatga acaatggggg cgcttcattg
     9541 gacggaatca gaaggaaatt caattaactt ctgaaccgaa gccttatacc attgaacgaa
     9601 cgttgaactg gcttggacga caagtcgccc ctacgtggaa gatggccaag gagttggatc
     9661 ggttgaatca gacaacgtac attcaggata tggtaaataa tactcgacta tcagaccggc
     9721 acaaaaagat tttggagcaa caaagtatgg caattgaaaa tttgattata tgaaaagagg
     9781 aatcgtgatg gaaattgtga aagatatttt attggtgttt ggtggcagta tgcttggtgt
     9841 tactgtgatg tgcttgatgc atgcaagtgc agcggcggac cgagcaatgg aaaagaaagg
     9901 aaagtgatga aatgaatttc ggtcaaaatt t
//
LOCUS       DAZKCY010000092         9550 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000092 DAZKCY010000000
VERSION     DAZKCY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.093"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACG8XC_002091"
     CDS             34..657
                     /locus_tag="ACG8XC_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191769.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACG8XC_002092"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACG8XC_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9191770.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACG8XC_002093"
     CDS             2153..3034
                     /locus_tag="ACG8XC_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9191771.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACG8XC_002094"
     CDS             3037..3807
                     /locus_tag="ACG8XC_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9191772.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACG8XC_002095"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACG8XC_002095"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP9191773.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACG8XC_002096"
     CDS             5118..6281
                     /locus_tag="ACG8XC_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9191774.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACG8XC_002097"
     CDS             6398..6808
                     /locus_tag="ACG8XC_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9191775.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACG8XC_002098"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACG8XC_002098"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9191776.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9348..9485
                     /locus_tag="ACG8XC_002099"
     CDS             9348..9485
                     /locus_tag="ACG8XC_002099"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191777.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLFITQVFHQYF
                     G"
BASE COUNT         3317 a         1614 c         2055 g         2564 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt ttattacaca agtatttcat caatattttg
     9481 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9541 ttcgtaagaa
//
LOCUS       DAZKCY010000093         9545 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000093 DAZKCY010000000
VERSION     DAZKCY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.094"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..977
                     /gene="gnd"
                     /locus_tag="ACG8XC_002100"
     CDS             75..977
                     /gene="gnd"
                     /locus_tag="ACG8XC_002100"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFP9191778.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            979..1980
                     /locus_tag="ACG8XC_002101"
     CDS             979..1980
                     /locus_tag="ACG8XC_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFP9191779.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1977..2261
                     /locus_tag="ACG8XC_002102"
     CDS             1977..2261
                     /locus_tag="ACG8XC_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP9191780.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2294..3091
                     /locus_tag="ACG8XC_002103"
     CDS             2294..3091
                     /locus_tag="ACG8XC_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9191781.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3105..3938
                     /locus_tag="ACG8XC_002104"
     CDS             3105..3938
                     /locus_tag="ACG8XC_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9191782.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3956..4813
                     /locus_tag="ACG8XC_002105"
     CDS             3956..4813
                     /locus_tag="ACG8XC_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9191783.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4835..5344
                     /locus_tag="ACG8XC_002106"
     CDS             4835..5344
                     /locus_tag="ACG8XC_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9191784.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5381..5782
                     /locus_tag="ACG8XC_002107"
     CDS             5381..5782
                     /locus_tag="ACG8XC_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191785.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5894..6247
                     /locus_tag="ACG8XC_002108"
     CDS             5894..6247
                     /locus_tag="ACG8XC_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191786.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6281..6724
                     /locus_tag="ACG8XC_002109"
     CDS             6281..6724
                     /locus_tag="ACG8XC_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9191787.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6741..7826
                     /gene="uxuA"
                     /locus_tag="ACG8XC_002110"
     CDS             6741..7826
                     /gene="uxuA"
                     /locus_tag="ACG8XC_002110"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9191788.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7819..8532
                     /locus_tag="ACG8XC_002111"
     CDS             7819..8532
                     /locus_tag="ACG8XC_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9191789.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8739..8888)
                     /locus_tag="ACG8XC_002112"
     CDS             complement(8739..8888)
                     /locus_tag="ACG8XC_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191790.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9284..9484
                     /locus_tag="ACG8XC_002113"
     CDS             9284..9484
                     /locus_tag="ACG8XC_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9191791.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3209 a         1496 c         2115 g         2725 t
ORIGIN      
        1 aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag ggtataagaa
       61 atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag
      121 ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg
      181 ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta
      241 cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca
      301 tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa ggcggaaact
      361 cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact
      421 tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg
      481 gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa
      541 gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg
      601 gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg
      661 agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg
      721 gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc
      781 ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg
      841 ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag
      901 atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag
      961 ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc
     1021 ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt
     1081 actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc
     1141 attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg
     1201 ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa
     1261 caccagacaa tcgtatctat catacctgct ttagttgaac gaaatgctgt agcagaaaat
     1321 aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag
     1381 gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt
     1441 ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt
     1501 cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt
     1561 gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg
     1621 aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa
     1681 aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat
     1741 ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga
     1801 cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc
     1861 tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc
     1921 agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga
     1981 tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag
     2041 aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg
     2101 tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg
     2161 aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac
     2221 gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa
     2281 actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc
     2341 tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg
     2401 acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc
     2461 ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag atacactagc
     2521 aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt
     2581 gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa
     2641 agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat
     2701 tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgtggc
     2761 agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca
     2821 tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa
     2881 aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat
     2941 gggattattt ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa
     3001 agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat
     3061 ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg
     3121 tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg
     3181 gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga
     3241 tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg
     3301 gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag
     3361 taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag
     3421 tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta
     3481 aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc
     3541 tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt
     3601 taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca
     3661 ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta
     3721 tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga
     3781 aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct
     3841 ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat
     3901 cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc
     3961 tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa
     4021 agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc
     4081 gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc
     4141 tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg
     4201 cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga
     4261 agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg
     4321 ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc
     4381 aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg
     4441 ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg
     4501 ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg aaatgctatc
     4561 agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc
     4621 attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt
     4681 gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg
     4741 attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat
     4801 cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt
     4861 ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa
     4921 ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa
     4981 aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt
     5041 ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca
     5101 aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat
     5161 tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc
     5221 agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca
     5281 agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc
     5341 ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt
     5401 tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa
     5461 acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt
     5521 cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa
     5581 agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag gaagttattt
     5641 catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat
     5701 tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt
     5761 attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt
     5821 cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc
     5881 atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc
     5941 gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa
     6001 aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc tacagttttc
     6061 gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa
     6121 aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa
     6181 aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt
     6241 tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt
     6301 tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc
     6361 gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa
     6421 ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct
     6481 aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa
     6541 gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa
     6601 tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa
     6661 agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt
     6721 ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt
     6781 cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc
     6841 tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg
     6901 ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca
     6961 aggcagggac agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact
     7021 tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga
     7081 aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa
     7141 tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat
     7201 tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt
     7261 atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga
     7321 ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg
     7381 aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg
     7441 cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg
     7501 aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca
     7561 gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag
     7621 gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta
     7681 tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt
     7741 atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa
     7801 aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt
     7861 ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat
     7921 tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga
     7981 gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc
     8041 aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca
     8101 gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat
     8161 acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa
     8221 atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct
     8281 tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg
     8341 atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg
     8401 ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat
     8461 aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg
     8521 ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact
     8581 gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg
     8641 aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat gcgtttaaag
     8701 agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt ttttaattct
     8761 tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc tttgtcaaaa
     8821 acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc tttgttatag
     8881 aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa cgcgttgata
     8941 acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta agaaatttta
     9001 aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg
     9061 ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta tatttcattg
     9121 cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct attttgaaac
     9181 gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa tatttattgc
     9241 aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata acggtacagt
     9301 aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg gaaatgatgt
     9361 atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag aaggccaagc
     9421 agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc
     9481 ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa
     9541 aaatt
//
LOCUS       DAZKCY010000094         9355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000094 DAZKCY010000000
VERSION     DAZKCY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.095"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..564)
                     /locus_tag="ACG8XC_002114"
     CDS             complement(31..564)
                     /locus_tag="ACG8XC_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFP9191792.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(658..1836)
                     /gene="mutY"
                     /locus_tag="ACG8XC_002115"
     CDS             complement(658..1836)
                     /gene="mutY"
                     /locus_tag="ACG8XC_002115"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP9191793.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(1829..2626)
                     /gene="recX"
                     /locus_tag="ACG8XC_002116"
     CDS             complement(1829..2626)
                     /gene="recX"
                     /locus_tag="ACG8XC_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP9191794.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2733..4115
                     /gene="rlmD"
                     /locus_tag="ACG8XC_002117"
     CDS             2733..4115
                     /gene="rlmD"
                     /locus_tag="ACG8XC_002117"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9191795.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4289..4867)
                     /locus_tag="ACG8XC_002118"
     CDS             complement(4289..4867)
                     /locus_tag="ACG8XC_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFP9191796.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5189..5658
                     /locus_tag="ACG8XC_002119"
                     /pseudo
     CDS             5189..5658
                     /locus_tag="ACG8XC_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
     gene            complement(5766..6992)
                     /locus_tag="ACG8XC_002120"
     CDS             complement(5766..6992)
                     /locus_tag="ACG8XC_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9191797.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7746..7946)
                     /locus_tag="ACG8XC_002121"
     CDS             complement(7746..7946)
                     /locus_tag="ACG8XC_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9191798.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8080..8688)
                     /locus_tag="ACG8XC_002122"
     CDS             complement(8080..8688)
                     /locus_tag="ACG8XC_002122"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFP9191799.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8773..9243)
                     /locus_tag="ACG8XC_002123"
     CDS             complement(8773..9243)
                     /locus_tag="ACG8XC_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFP9191800.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
BASE COUNT         2752 a         1884 c         1536 g         3183 t
ORIGIN      
        1 ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct tgtaacggtt
       61 ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga tccagtcaac
      121 cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcgggtaat tcatcatttt
      181 gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag gaaatacctt
      241 gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa aaggtgaagc
      301 tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat tgaaccagta
      361 ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc catcagattc
      421 agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt ttaataccat
      481 ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga tcgtcacaaa
      541 ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt gtgctttgtg
      601 tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga gctttgccta
      661 gaaaggattc ctattgtttc gatttttctc taactgatct accagtttca tttggacctt
      721 aggaaaaacg accgaatcaa atgcatcagg cttcaaccac ttcgaagttt tgttctcggt
      781 aaattcttgt tctgctccct cggttgcccg tccgtaaaaa aggagaacat gccatttcag
      841 atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc tgccaaacaa agagttcctg
      901 cttttcaaat gggagatttt gctcttcttc tgctactagg tcatcaaaca gatcgatttc
      961 tggctttgtt tcccattctt ttttcaatcg ctcatactcc tcttgtgtca cttccatcat
     1021 tggaaatgtc cacatattcg ccagcaattt ttggctatct ctttcttcaa aataatacgc
     1081 tccagaatga ttttgaagag ctgcactgat ataataaacg tcttttggct ttgctttttt
     1141 tgtttttaca ggaaaactcg tttggattcc tcgtttgtta gctaaacaaa aggcttggat
     1201 cggacaagtc tcacactttg gagaagttgg tgtacagata gctgaaccta gatccatcat
     1261 tgcttggttg aattcacctg gatgcttttc atcaatgatt ttccgcatcg cttcatcaaa
     1321 gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg caaaacaatc tgctgactac
     1381 tcgcatcacg ttgccgtcaa ctgcaggttc tggaagtcca aatgcgatac ttgcaatcgc
     1441 gcctgtcgta tatggtccta ttcccttcaa tgagctaatt tcttcaggcg tttgcggcat
     1501 ttccccatca aattctgaca tgatctgttt agcagcagct tggatgttcc gggctcttga
     1561 gtaataacca aggccctccc atgcttttaa aagcttttct tctggagcat ttgctagctc
     1621 ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa tcgatcactg tgtctactct
     1681 ggtttgctga agcatgatct cagagatcca gatacgataa ggatcacgat tatagcgcca
     1741 aggcaggttt cttttttctt gttcatacca ttgaatgaac tgatcttgaa attcttttgt
     1801 ttctttatct gtccattttt cccattcttt attcactttc ctcttccttt tccgtaatga
     1861 atcgctggat catatccaaa tcaaatcctt tttgatataa gctttgcttt acttttagat
     1921 ttcttttttt aggttcaaac cttaaatttc ttcgccatag tttatctcct tgtttgacta
     1981 aataatcata ttctatttct tgatctgctt cttgcggaag atcagctatc gcttgttgga
     2041 tcacttcttg agaaaagcct ttcgtcatca atgtttgttg gatttttctc aataattctt
     2101 tttggctttt tccgtgattt tttccaaacg ctttttcggc tgtctgatgt gcattttgaa
     2161 cttggtcttc aaatgtatat tggctgagcg cttgttcaat gatttcaggt ttcaaacctt
     2221 tttgctgcat ctgctgcgct aatttctttg gtcctttgtc acttaagcgc atattcgtgc
     2281 gcacataact ttccccatag accaaatcat cgacaaggtc caattctttt aagcgggcta
     2341 cgattttatt tcgatcttct tgaaggattt ccttttcttt taagtatgtt cggatttctt
     2401 tttccgatcg caactgataa cttaagtaat tcattgctag gtgaaaaccg aaatcagctc
     2461 cgctactctt cttgatctct tggatagttt cttcgtctaa ttcccgtcct tttaataatc
     2521 gatagcgtac taatacatct tcagatactt gtaaagcttc tccttcagat aaatctacgc
     2581 gatagaaagg gcctttgcct ttactgattc tcgtaacggt taacataaac atcctcctcg
     2641 aacatatatt ctctatcata ccacagaaaa tccccgtaat caggtattat atgttagaat
     2701 aaaccacaga aaaaagaaaa agggagatac ttatggcaga aacaattgtc aaaactggac
     2761 aaacggttcc tttgaaaatc aagcgtttag gcataaatgg tgaaggaatc ggttatttca
     2821 aacggttgat catatttgtt ccatatgcac taccaaaaga agaagtacta gtgaaaatca
     2881 cgaaagcaac tcctcgctat gcagaaggag aattaataaa agtcaaaaaa acaagcaaag
     2941 atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg tggctgtcaa ttacaacact
     3001 tagcttatca agctcaattg gattttaaaa aggatctgct tttgcaagca ttggaaaaat
     3061 tcaaaccagc aggctttcga tcttatcaac ttttgccgac gatcgggatg gatgagcctt
     3121 ggcattatag aaacaaagca caatttcagt tacgaaaaaa taagcagact caaaaaattg
     3181 aagctggttt atacgaagca aactctcatc agttagtccc tataacagac tgcttggttc
     3241 aagaacctcg aacacaaaaa gtaatgaata ctgttgttca actattgaat aaatttgatg
     3301 cacccatcta tgatgaacgg acaaacagtg gaatcttccg cacaatcatg gttagaatcg
     3361 gtgttaaaac tggggagtta caagttgttt ttattactcg cagtaaaaaa ttcccgcaaa
     3421 aaaacgcaat gatccgtgag atcaatgaac gactgcccga agttgtttca atcatgcaaa
     3481 atgtccaggc acaaaaaacg tctctcgtga tgggggatga aacgatccac ttatggggaa
     3541 aagaagccat tgaagaacaa atcaatgaag tgactttcga tctttctcct cgtgccttct
     3601 ttcaactcaa tcctcagcag accgaattgc tttatggcga aggaatcaaa gcattagatg
     3661 tccagcctaa tgaaacgatc gtggatgctt attgcggagt tgggacgatc ggcttaagtg
     3721 tagcaaaaca agccaaagaa gtacggggaa tggataccgt tcctcaagca atcgctgatg
     3781 caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg atatgaagtc gggacagcag
     3841 agcaagttct gcctaaatgg ttgaatgaag gttttcagcc tgatggtatc atcgtagatc
     3901 caccacgtac gggtttggat aacgcattga tccaatcatt attgaaatac cctccaaaaa
     3961 aacttgttta tatctcctgt aatgtttcta cacttgccag agatctggtt aatttaagta
     4021 aaaagttcaa gattgaatat ttacagtccg tcgacatgtt tcctcagact gcacgttgtg
     4081 aagttgtcgt gaagatgacg agactcgctg actaaatgaa agaggttgtg aaaaagtctg
     4141 ccctgtatca agtaataaga acagccaaac aaaaatagct cttcatgttt tatgaatact
     4201 gtacttgtga ccgaagagtt aacaggagga cactatttat ttaaaaaaag ggttgtgaca
     4261 tagtttttgt cacagccctt tttgattatt attggatatt cacgtctatc acttgataaa
     4321 atgcatttgt cgtatcgctg atattccata ctgctaaaat cacattatat ccttttcgat
     4381 cctgcggaag tgtgatcgtt tggttgactg tttttggtgg aacagttgct ttgtcgtcta
     4441 tttttgtgat caattcaaaa tcactaaatt tcaaaggttg attaggattc caagttggtt
     4501 tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc tgttaaattc caagtaatat
     4561 ttaatgttcc tggattaacc atgtttttat gccatctgtt agatgtctgt tcatctaaag
     4621 gctcaaagcc gctgactcct gcactagcaa tttttccatc aatgaatgta tttttggtag
     4681 cttcgatact ttgcggttcc cattgtgctc ttccgacatt ttgatttaag tttcctgctg
     4741 ctgatgttcc taggtatgca cggcttcctg gagaagtaat ataaccatgc gcaaaacctg
     4801 gaatgctaaa accgaacaac cctaaactaa ctaacccaat aaacaataac gtactaatct
     4861 ttttcatgta gaaacctcct tcatttgata accacattgt aaacgcttta ttgtattaac
     4921 acaaacatta tgcttttata attttatgtt gaagtaaaaa aggtgaaaaa agtaagatgc
     4981 agattataac agaagtagac gagaaaagta caaatagaaa acatgagaat caaatcatat
     5041 gatctttttc aaaaacatca ttcatataaa caaatgttca actttttaat aacaacaatt
     5101 atcaattttt ttcttacttc acaaactaaa tacaggtctt gctgtgagcc ttcatggtaa
     5161 actaatatta tagaaaggag ttagtaaaat gaagatgata aaagatattc gtttttgggt
     5221 ttgtgttata ggaattgtca tattaggctt tttatcaggg ttgcttagcg gaaatcccgg
     5281 aaaatattac tattctttgc aacttccacc atttgcacca cctagctgga tatttgggcc
     5341 gatgtggacc ttactgtata tattgatggg tatttctctt tatttgttac ttaatcacaa
     5401 caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg attcagttca tttttaattt
     5461 atatggtctg ctctattttt taatttaaga aatattttca ttgcagcaat cgatattaca
     5521 cttttagtta ttttcttgtc agtgcttatg tatcagttgt ggctgcatca tcgcctggca
     5581 atgtggctca tgattcctta ttatttatgg gtactattcg caactctatt gaattattca
     5641 atttattttt taaactaagc gctaaaaaga gacgtcctgt aatttaacac atagaaaaag
     5701 tgccacctaa gttagatttt ttgtctaatt taggtggcac tctttattaa ccaaactatt
     5761 tttttctagt acccacttgt tgttccatac tcattattct ggtcataacc ataaccattt
     5821 tgttcctgtc cagcgtaagg atcataactt tgagtatctt gacttggtgt atagtactga
     5881 cttgtatctt ctgtttgctg ataataggta ttcggatctt ggtactgatc atatgtgttt
     5941 tctgaatagg aagaataatc ggtgtaccct tcactattcg atccatcacc gtttgtcgaa
     6001 tcgccatctt ccgaatcatc aaagaacatc attgaagcat tctcattttt caaattaggt
     6061 aatgtatcgc ttgtttttat atttaattgt ttcttcagtt cattttggat cgaaagaaga
     6121 tcattttttc ctaaaatttg atagtagata tcattgatca tttgtccttc gccttgcaac
     6181 tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg tcgctacatc aagcatgtca
     6241 tcccaatcta agtccgtcgt cacattgttc tcaactgctt ttaaaatttt tctataattg
     6301 cttacgccat ctaaacttaa aactttattg acgattttcg tcactacttc ccgctgacga
     6361 cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca ttcgagaata ggctaaacct
     6421 ttttctccat tcaatgtttg ttcacctact ggtacatgga caccatccaa agtaaagtca
     6481 attttattat tcactttgat gccaccgacc gcattgatca aatcttccat gccatccaaa
     6541 ttgatcgtta cataatgatc aataggaata tcgagcagtt gctcaattga atccatggcc
     6601 atttcgacac caccaaatgc gtaggcatga tttaatttat ctaccgttcc ataacctacg
     6661 atattcgtat aaatatctcg gtccaaactg atgatcgttg attttttttg tttcggatta
     6721 actgttacta ccatcatcgt atcagaacgt ccttgttccg ttcttcctaa tccaccggta
     6781 tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat caatgcttac tctctcagaa
     6841 cgtttggagg atattcgatt agagttttta gaaattttgt ttactgtttg gctcgcttct
     6901 cccataaatc tgattccgta tgctgataat ccggcaatca gcaaaacgaa tacccctaac
     6961 aaggttaata gaactttttg ccatcgcttc ataatacacc tctgtttcac attttctaaa
     7021 atattgtctc atataactgt tcaatcagca aattcacttt ttaatcataa gaaaacttta
     7081 aaaaacattt ttgtgtaacg attattattt tttaaaattt ctcaaaattc ttaactatca
     7141 taccataccg tgaaaaagat cgaaagaccg aatacacgca gttcgcaatc gttttatctt
     7201 tttttatgta ttttttccag aaaaagcgaa caacaaggta ttttccaaat aatcgtgcag
     7261 taaaatgatc gcacatacct ttcccatctt tttaacagtc tttgcctacc gttcagatta
     7321 tcattcgatt tcacgacaat ttcttcctat gactggtcct tttgttaaac ccatcttcta
     7381 ttcgttttat ttccagctca ctgcatggct cttaaaatga ctcgaactag tgattgcatt
     7441 accatcatct tttaatggat agatatgctc ctcttcaaag taaaacgatt ttcttttaaa
     7501 gtaatagaat tgacatggta atgagtatgt atactagtgg tttcacaaat ggtcttttat
     7561 tcaacatcca aaaactgaaa tttctttaaa atccattact aacgaagaaa atgttccttg
     7621 cttgataatt agttaggaca tgtcaagtga acaaatgcat ttacttagcc aaaaaatcta
     7681 aaaacttcat aaatagacaa aaagacacga ctctctaaga agtcgtgtct ttattcaaca
     7741 aattattata atttagtcac ttcagcagct tgaggtccgc gtgcaccttc aacaatcgtg
     7801 aattctactt cttgaccttc ttctaggctc ttgaagccgt ctccttgaat tgctgagaaa
     7861 tgaacaaata cgtcatctcc gccgtctact gtaataaacc caaatccttt ttcattgttg
     7921 aaccatttaa ccgttccgtg ttccataaat gtatatcctc caaaaaagtt cttgcaagta
     7981 accttgcaag aacattatac ccaatttaga aaacgctagc aagctttcgc tcttttattt
     8041 tccaaattat ttttaagact gatatgacgg aggaaatggt tattttgctg cttcgaaacg
     8101 tttgtttact tcatcccaat taataacatt ccagaatgct gcaatataat ctggtcggac
     8161 atttttatat ttcaaataat aagcatgttc ccaaacgtcc aaacctagaa ttggtgtctt
     8221 tccttccatc aatggagaat cttgatttgc agtagaggta atagctaatt ttccattttc
     8281 cattacaagc caagcccatc cagaaccaaa tcgtccagcg gctgcttttt tgaattcttc
     8341 tttaaaagaa gaaaaatcac caaaagcttc attaatcgct tcttttattt ctcctgtagg
     8401 ttcgccacca gcatttggtg ccatgatttc ccagaaaaat gaatggttag catgtccgcc
     8461 accattgtta cgtacagctg tcttgatatc tgttggaata gcgtccatat cagataataa
     8521 ttcttctatt gttttttcgc ctaattctgg gtatttctca attgctgaat ttaaattcgt
     8581 cacataagta ttgtgatgct tatcatgatg cagatgcatc gtttcttcgt caatataagg
     8641 ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg taagtcataa aaaaattcct
     8701 cctcaatgtt ttcttaataa tgtctacatt acaaagagta acaaagagaa ggaaattctt
     8761 caattgttat gcctagtcgt ctttttcact ttgacctact tcttcttcaa aaacctcagt
     8821 ttctttaaaa ataaacaact tgctgaaaat atagttcgca aggataacaa taatttgagt
     8881 gatgattttc gccacatact cattgctgtt cataacattg atcaacaata ccattgcccc
     8941 catgtccaat ccgtaagaaa ggatacggta gaagaagaac ttaccgcatt caataagtgc
     9001 atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt ttgttcgtta caaaagcaaa
     9061 caatacagaa caaaaccacg aaatagaatt ggataaggcc atacttaaat caaaatattg
     9121 gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc aaaccaccaa aaaagagata
     9181 gactagtatc tcccaatatc cttttgcttc taaatatcct tttaactgtc gatactgctt
     9241 catgagccaa cctaccttat taaaaattct tatttatcat actctatatt tcaataactt
     9301 tcatctttat ttaaaaaaat taattttttt tgatcctttg cctttgtctc tgctt
//
LOCUS       DAZKCY010000095         9292 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000095 DAZKCY010000000
VERSION     DAZKCY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.096"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..110)
                     /locus_tag="ACG8XC_002124"
     CDS             complement(<1..110)
                     /locus_tag="ACG8XC_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9191801.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
     gene            187..1776
                     /locus_tag="ACG8XC_002125"
                     /pseudo
     CDS             187..1776
                     /locus_tag="ACG8XC_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing transglutaminase
                     family protein"
     gene            complement(1840..4011)
                     /gene="copB"
                     /locus_tag="ACG8XC_002126"
     CDS             complement(1840..4011)
                     /gene="copB"
                     /locus_tag="ACG8XC_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFP9191802.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(4031..6217)
                     /locus_tag="ACG8XC_002127"
     CDS             complement(4031..6217)
                     /locus_tag="ACG8XC_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9191803.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDALFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(6217..6426)
                     /gene="copZ"
                     /locus_tag="ACG8XC_002128"
     CDS             complement(6217..6426)
                     /gene="copZ"
                     /locus_tag="ACG8XC_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFP9191804.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(6439..6879)
                     /locus_tag="ACG8XC_002129"
     CDS             complement(6439..6879)
                     /locus_tag="ACG8XC_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFP9191805.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(6953..7451)
                     /locus_tag="ACG8XC_002130"
                     /pseudo
     CDS             complement(6953..7451)
                     /locus_tag="ACG8XC_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            complement(7736..9202)
                     /locus_tag="ACG8XC_002131"
     CDS             complement(7736..9202)
                     /locus_tag="ACG8XC_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9191806.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2634 a         2017 c         1629 g         3012 t
ORIGIN      
        1 gttagttcat attctcgaat gatttctaca cgtggctttc cagctaagta aagattgacg
       61 atttgttgct tgaattcttg tgaaaaagtt cttcgtgttc tcttagacat aaaaattcct
      121 cctggtatgt tttcttctag tctacacacc ttaatttttc tgtctagtta attgtagcct
      181 atccaacacc aatctctcga ggaaacgagt gaaccgatcc gagaatatct taatgatgaa
      241 gggctttatg acacactcca tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt
      301 gagaatgatg aacagcttgg tggaccttta tatgataatg gcgaagttgt ttttacagcg
      361 aaccaaaaag gaaatggcta ttggaagatc gaaaataaaa atcattacac cggttctgga
      421 tggatagaag atcaggacgc acccatccaa ccaatccaag aattccctgt tgacatcacg
      481 agctaccaaa ccgttataga agacaaagaa aacattacat tgaccctaaa aaaacaagct
      541 ttcttacctt atccttatgg aaatactacc ttgactgatt tttcattagc tagtggacaa
      601 acgctttact atttgcctga aagccagcga ttcattcctg tcccgaatga atcgacacgt
      661 tctgcaactt tacaaacaga aaggttagtt tttacgagag aagaattaaa aagcagtcag
      721 ttaaaagctg atgatttaga aaatcagcag acaaatcttc aattgccaaa aagtttgcct
      781 aaacgtattg aggacttggc ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa
      841 gtcgaagctg tctaacagta tttaaaaaca agcggaaatt tccgctattc caaaacggat
      901 accgggcata ctccgaaaga acacgattat gtggattatt tcctctttga ttcaaaagtc
      961 ggctattgtg acaacttttc aacttctatg gtcgtccttc ttcgaacact tggtatccca
     1021 gctcgctgga caaaaggttt tacaaatggg ctcagaacag gcacagataa tcaagggaat
     1081 gaggaataca gcattctcaa tagtcatgct cattcttggc cagaggtata ctttcctgga
     1141 tttggctggc ttccatttga accaacccct tcatttgcca atccaatggg ttcacaacca
     1201 gttgctgaaa caaccgaaac cgtccagcct gctatcgaac aaccaaatga aagcgaaaat
     1261 atggcagaaa ctatttccag tgagagatct gaggtcattc catcagaaga aacaacactc
     1321 acaccagctt ataaaatcgt cttattcagc cttggagtgt gtttatccgt cttgttgttc
     1381 tttctattgt tattcagaaa atctctgcgc tggacaagtt atcttatcta tcttcatttt
     1441 tttgcttcta ctaatacgat tgcatatcaa aaacttctta accctttgga aacattggta
     1501 ccgcgcaaac ccgcagaatc tttgcaggaa tatagcctgc gaatagaaca agaattcaag
     1561 ctagatctgc gtttcattcg attaactact caatatgaag cagaaatata tggacgacaa
     1621 gcagatccgg taaaattcga taaaaaaacc tatttagaaa taacgcatat aataagaaaa
     1681 acggtataca aaaaaattct gacaaaaggg agccgcttca aaaaacaaga taaaatttct
     1741 ggagaatcta cttctcgaat gccgagaacc agctgattga gtatcgatta ctcgaaaaaa
     1801 aggttgtgac acgttttttg tcacaacctt ttttcattat tatttgattt tcaatgtcaa
     1861 agcatttaga gctacaacta ccgtacttaa cgacatcaag acagcaccaa ctgctggact
     1921 taatatgata ccaattggcg caagaattcc ggctgctagc ggaattgcaa gaatattgta
     1981 acctgccccc caccagaggt tctggatcat tttctttcgc gtttgcttgg ctagatcaag
     2041 aaaatgcaaa atatcttttg gatcgctgtt tgtcaatacg acatccgcag aatcgattgc
     2101 gatatctgtc cctgcaccga ttgccatccc gattgaagca cgggctaaac ttggcgcatc
     2161 attgatccca tcacccacca tgattacttt tttcccttgg tcaaggtatt tctggacaac
     2221 agcttcttta tcatctggta ataaaccgcc ataatattct tcgatcccta aataatctgc
     2281 tactgcttgg gcagcgtttt tattgtcacc agtaagcatg acaggagtga tttttctttc
     2341 tttcaatgtt cggataaatt cttttgcttc tgtttttact acgtcaccta aagcaacgat
     2401 tgcagctaga tgattttcta aaatcaggaa gctgatggta tttccttgtt cttggtaagg
     2461 tttcaataat gcttgttcta cgtttaatcc gagacgctca acttcttttt cattaactat
     2521 tttcacttgt tgttttttga ctgttgcctc tagcccgact cctgaaagat tttttagatt
     2581 ttcagcttga taaggcttga tttcatgttc tttcaaataa ttcataatgc caacagccaa
     2641 tggatgattg gcattcgctt ccaaggctcc tagatattga aggatttcat ttttattgaa
     2701 cgcatcatca agtacttcga cacccgttac agtaaatttt ccttctgtta gtgttcctgt
     2761 tttgtccagc atgatcacat ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa
     2821 aagtaatccg tttttagctg ctattgatgt ggagcgagca acaactaatg ggatagctaa
     2881 acctagagca tgaggacagg cgatgataaa gactgttacc atacgttcta atgcattagg
     2941 aagatctgtc aagaacaacc aggcgatgaa tgccaggata cccacaatta atgcgacata
     3001 aaacagccat tttgccactt tgtcagataa tgactctagc ttggattttt ctccttgtgc
     3061 ttttcgcacc atttccataa ccttcgctag ataaccattt tcaccagttc cagttacttc
     3121 gatttcgatc gttccatccc cattgataga accgccaatc actttgtcat tggcttgttt
     3181 tttgattcct cttgattcgc cagtcacagc tgattcgtca acaattgttt cacctttcaa
     3241 gattacacca tctgttggca ttttatcacc agaccggacg atcaaacgat caccttcgtt
     3301 tacttctttc aatgaaactg tctcttcttt tccatctttc gtcaagcgtt tcactgattc
     3361 tggcagtaat tctgctaatt tttgtaaagc attgctggca ttcgacacag cattcatttc
     3421 aatccagtga cctagtagca taataacgat caaagtagcc aattcccaga agaaatccat
     3481 gacgtgtgta tgtggattga gcagatttgc gataaacgaa tagacactat aaatatatgc
     3541 aactgtaatc cccatcgcga tcagtgtcat catagcagga cttttttgtt tcaattccat
     3601 cttagctcca cttaaaaatg gctgaccgcc atagataaat agtatagttg ctaagatcaa
     3661 tacgagccaa tcagaaccag gaaacgttac ttgaaatgga aattccattc ccatcattgg
     3721 tgaaaaaaga atgattggga tagctaggat aagcgatagc caaaattttt gtttgaaatt
     3781 ccccatgtgc atggaatgat ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc
     3841 catttgtttg ctgtttgcta tatctccatg gttatgtccc atgtgcatgg aatgatccat
     3901 ctgactatgg tcatgtttca tctccatctg tttttcatga gattctgtac tttctttgtt
     3961 tactacttct tttttatgct catgtttttt ctccatatcg ttcccattca tttttactcc
     4021 atcccttctc ttactttttc atgtgtcgat ttaagctcag ggagttcaac aatacactga
     4081 cggaactaaa cgccatcgct cctcctgcaa tgattggatt caaaaagcct agtgcggcaa
     4141 aaggaatacc aatcgtatta tagacaaaag cccagaaaag attttgtttg atttttctca
     4201 atgtcaacct agacaactcg attgtttgtg caatggatgc caaactgcta ttcatcagcg
     4261 tcacatcggc tgtttccatc gcaatgtccg ttccacttcc cattgccata ccgacatcag
     4321 ctaatgctaa ggccggtgca tcattgattc catcgcctgc cataccgact tttttgccat
     4381 ctttctgaag tttttctacg taggctgctt tatcctctgg aagtacttca gcaaagatat
     4441 gctttggatc aataccaact tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg
     4501 tcagcataaa aacatctaat cctttattct ggagctgctt gattgcttgt ttggcttcta
     4561 gtttgatttg atcagccacg gcaatcaacc cgatcacttg cttttcatca gctaaaaaca
     4621 tgactgtttt tccctgctgt tctaattcta acgcgtgttc ttggtattca tcaaatgata
     4681 ggttcaattc aattaatcgt ttacgtgtac ccgcaaaata gcgtactcca ttaatcgtac
     4741 cgctgatccc agcacctggg tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt
     4801 gcagttttcc gtattccacg attgctttac ctagtggatg ttcagaagct tgttctaaag
     4861 tataaaaaat agataggact tctttagaac cgactagatc tgtaactttg ggctttcctt
     4921 ctgtaatcgt tccagtttta tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg
     4981 cttctccgcc tttaattaaa attccatttc gtgcaccaac acccgttcct accataatag
     5041 ctgttggagt cgctaaacct aatgcacatg gacacgcaat gactaataca gagacactat
     5101 ggatcaatgc gagttgccaa tcaccagtga tcaaacctgt agctatgagc gtgataaacg
     5161 cgatgactaa tacgatcgga acaaaaatcc ctgagatccg atctgcgatt ttttggattg
     5221 gtgctttgct cccttgagca tcttccacca tttgaatgat ttgcgctaat actgtctgct
     5281 tgccgacttg tgtgacttcc atgtgtagca gaccattcgt attgattgtc ccgccaaaaa
     5341 gcgcgtcatc cggattcttt tctacaggaa gattttcacc agtaagcata ctttcatcta
     5401 tcgcactgct tcctgaaata attcgaccat ctgctggaat ctgctcacca ggacggatga
     5461 ctagttgatc accaacgact acttcttcaa tcgctaacgt ttcttctttc ccgtttctta
     5521 tcacttgggc ggtttttgtt tgaagagaca tcaattgttt gattgcatta cctgttttcg
     5581 tttttgctgt atgttccaaa tatttgccga gcaaaatcag cgtgatgatc atactgctgc
     5641 tttcaaaata aagatcttgg gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa
     5701 aggcagccga tgttccaatt gctaccaata catccatatt tggtgctttc gtcttgattg
     5761 catggtacgc acctttataa aaacgagcac cgacataaaa ttggacagga gcagacagca
     5821 ctaactggac gattggaaaa tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca
     5881 tagcaatcat tgacaacatc aacggcaaag tcaaaaccgt actcaatata agatcgaata
     5941 gcattttttt caaatacgct tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt
     6001 acaaaattgc accatagccg atgttttcta cgctttggat taatttttca gtggtcgtcc
     6061 cttcatattt gacacttgct ttttcagttg ccaagttgac tgtggcattg accacaccag
     6121 gttgtccgtt cagctctttt tcgattctag cagaacaatt tgcacaagtc atacctgtaa
     6181 tgacgaacgt atccattttg gtttctgctg ttgccatcaa atcacctctg ctttataacc
     6241 caatccattg attgcttgac aaatcttttc ggattgaact tttgtttcat caaacttgac
     6301 cgtaccgttt gcttttttca aatttacttt cactttttgc acgccgtcaa gtgcgcttac
     6361 tgtttcttct actcttgctg cacaatgatt gcagctcata ccttcgattg aaaattgttg
     6421 tttcattttt tcttcctcct attgatgttg tttacattca cattgacctg ggatacagtt
     6481 acattcgatc gtatctactg gttgcttttg ttccagttgt ttcaaaatct cattgatatc
     6541 ttcttgagtc aaagtggctt cttctactag atccgcaatc gtctctccaa tcctttttgc
     6601 acagatatgg gaaaaaagat tttccgtcgc actgcgaact gtttccatct ccgagacagc
     6661 tggatgataa atgaattttt ttccttcttg ttctgtccag atcacttctt tttttaccaa
     6721 acgacccagc aaagttttga tcgttgctgg tttccaatcc atcgaatcag ctaagatctg
     6781 cgtgatttct tgggctgtcg tctgtccaag tgtccaaata acacgcatga tctcccactc
     6841 tgaatcacta atctttactg gcgttttgac tttttccatt tatttcacac ctttcgttta
     6901 caaatgtaat cattaactta tgaatacaga ttacacttgt aaacaaaaac tgtcaattat
     6961 ttttgctctc tattttcaat ttagttaaaa attcttgttt ctctcgtctt gttttaagga
     7021 tgatcagttt atttcttgca ttagctactt ggatcagctg ctgtattttt ggttcattgt
     7081 ttccttcaaa atgccagata aaagataaaa attctaagta tttcctgttt agtttctccg
     7141 aaaatgattc aggcatatct ggccgagatt ttttgtttat ctttcttttt agagaacgcc
     7201 aaactactcg gtagatggcg agataacgcg gcactttaag ccaaatgatc ttatctgctt
     7261 ctttaatcct ttcctctaac gtagaagtat aatttccttc aacgatccat gacggattag
     7321 ataccataaa cgattgctgt tcttgcaaaa accacttttt tgcctgaatg cggaaagccg
     7381 gtgatttctt tgatcttcct tgcgagggac gattttcccg acccactttg tccaatcacg
     7441 atgatcttca ttcttctccc tcacttctct ttggaatttg gttcgatcat accacttata
     7501 acgccgcaaa aaaacaaggg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaata
     7561 aatggtgttc aatcagctga ccttcagcat tagctcaaaa aacggcaaaa tatgaggagc
     7621 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
     7681 cacggtgttc cagaagtagc ttctgtcaca acctcttgta acaagtattt ctattttaag
     7741 gctgaaccac tgtttctggt tcagtaattt ctttgttttt gtctcgacga gcaattgctg
     7801 gcaagatcat acctaataag atcaagacaa ttggtgtgat gatattcgtg aataattgga
     7861 accaccatgc tttaggatca gcagtataat ctacttttgg caccattcct aggatacatg
     7921 caaacgcagt gaaggcaaag caccaagcac caaagataaa accgattttt ggatttttaa
     7981 caaatttata ttcagaattg aatttcttat aagctttatt caacatcata tatgccaaga
     8041 acacccatag gtaacgcatt ggcatgacaa ctgagttcaa gttcgtcaac cattttacta
     8101 attcattcat atctttgata ccaaagatcg gcaacaaaat aatgattcct actagtatac
     8161 ctgtcagaag ataaccattc accaatgtac ctttacgatt acgtttacgt agccagtttg
     8221 gtacaaattc tggatccgca tctgctaaca agatttttag tggtgcatca attgaaaagg
     8281 ctaatgcgga gatttgtcca atcatgtttg tcaatgcgta aatgatcatc aatgagttac
     8341 cgataccata gtaattacct aaaatttgaa aagcactgta tgccccattt gccatcaagt
     8401 ctttagggat attatcgcta gcaaacagca tacccattgc gactgagcct aatacagcac
     8461 aaatccctac cattgctgct aagaagatca tccctttagg aaattccttt gcaggatttc
     8521 ttgtagagtt gacataaggg gatatttttt ccgcccctcc aactgcaaat actagcatcg
     8581 aaacggttgt gaaataactg aaatcaaatg ttggaatata tgttttcact tgattcatat
     8641 gtggtgtggc aaattccatc gttggattga tgaacggtgc cgttacagct aaaagaataa
     8701 ataagatcga catgacgaac atcgctgttc cggccagtcc gccaatcact ttcaaagtag
     8761 aaagtccttt tgttgataaa tataaaaagg caagaaatat aataagtgaa ataactgcta
     8821 ccatttgtac tgacatcgta tttacgatat ttccatttcc ttggactgcc caaccaccag
     8881 caatcagaat tgcttgaggc ttttgtgcca aataaggaat atggacgacc cagtaagtcc
     8941 aagcggcata gtatgccagt cgtttattag atgtcgtatt ttcgacccat gaactgacgc
     9001 ctcctttact atccttgaag gctgatccta attgtccaac gatcaatgcg tatggaataa
     9061 aataaatggc caaaatcaat agccaagagg tgacaactga tactccttgc tgagcatagt
     9121 tattaactac attccccatg ccccaaacca tgttaaatgc aattaacgca acagtaaacc
     9181 agcgtaattg tttgttgttc attcgttctc tcctttatga taaaaatttg ttatccagat
     9241 gtaatcatat tcaattttta acaaacttac aagacagtgt tattttatgt gt
//
LOCUS       DAZKCY010000096         9184 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000096 DAZKCY010000000
VERSION     DAZKCY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.097"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..916)
                     /locus_tag="ACG8XC_002132"
     CDS             complement(92..916)
                     /locus_tag="ACG8XC_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFP9191807.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(889..1569)
                     /locus_tag="ACG8XC_002133"
     CDS             complement(889..1569)
                     /locus_tag="ACG8XC_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9191808.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1592..2530)
                     /locus_tag="ACG8XC_002134"
     CDS             complement(1592..2530)
                     /locus_tag="ACG8XC_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP9191809.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2775..3626)
                     /gene="ispE"
                     /locus_tag="ACG8XC_002135"
     CDS             complement(2775..3626)
                     /gene="ispE"
                     /locus_tag="ACG8XC_002135"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP9191810.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3780..6353)
                     /gene="mprF"
                     /locus_tag="ACG8XC_002136"
     CDS             complement(3780..6353)
                     /gene="mprF"
                     /locus_tag="ACG8XC_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP9191811.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6494..7300
                     /gene="proC"
                     /locus_tag="ACG8XC_002137"
     CDS             6494..7300
                     /gene="proC"
                     /locus_tag="ACG8XC_002137"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP9191812.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7379..8935)
                     /gene="rny"
                     /locus_tag="ACG8XC_002138"
     CDS             complement(7379..8935)
                     /gene="rny"
                     /locus_tag="ACG8XC_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFP9191813.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         2721 a         2020 c         1578 g         2865 t
ORIGIN      
        1 gaaaaaataa gaaaatagtt cggtatttat taaaataaaa agaagcctat aaaaaatttt
       61 tcgtttttat aaggcttctt ttctttataa tttagttatt ttttcttctg aaggcaacca
      121 atattcgttt gatgatattt acaatcagga acaaagcaat aaagactaat gtgattgttg
      181 ctcctggagg tgtatctaga taaaacgagc tagttagtcc tgacagcatc cctagaaatc
      241 ctacacaaac actgatagca atcacgacat tgaagctttt gcctaatcgc ataccgattg
      301 ctgcgggaag aaccatgatt gcggatatca aaagtgcacc ggcaatcggg atcatcacag
      361 caatagccac accagtaatc acattgaaca tcatcgacat caaatgaacg ggtagcccat
      421 ccacgtgggc ggtatcttca tcaaaagtca gaacatacat cggccgtttg aataaaaaga
      481 acaatatacc gatggcaacg aataagattc ctaaaaagat gacttgctcc catgtaatgg
      541 tcacgatcga accaaacaga taggattgga tacttgttgc tgaattccct ccgctcaaat
      601 tcatcaatac taacgctagc gccaaaccac cagccattaa aatggcaatg gagatttctg
      661 aataggttcg gtagatcgtt cgcagatact ccaatatcac agctgctaat acaacgacta
      721 gcatggtcgt aagtgtagga ttaaggttca aaaagaatcc taacgctacc cctgctaaag
      781 agacatgaga aagcgtatca gccatcagcg attgtcttct gatcactaaa aacacaccta
      841 gcatcggggc gattccggca atgaagatag cggcaagaaa cgctcttctc atgaattcat
      901 atgaaagcaa cgccattgag aatcctcctt tctcaccagt cggatctgtc tgtccgcata
      961 ctgtttgata tcttcatgat catgggtgat cattagaact gatttcccat ggacatgcgc
     1021 actatgtcgg agaagacgat aaaattcatt tctggattct tcatccatac ctgttgttgg
     1081 ttcatcaaga acaaataagt cagggtcagt agcaaaaata cgcgctaaac tgattcgctg
     1141 cttttgtccg ccagacaatt cacctattcg ccgatttcgc atatcccaca tccctacagc
     1201 ttctaaagca cgctgtacat gttgatgatc tttttcagag agacgcttga accagcggcc
     1261 tcttggatat cttcctgaac ggactaattc gatcaccgta ctaggaaaac ctgcattgaa
     1321 agatgcaact tgttgaggaa tataaccgat acttaatttt tttccttctt gatttttctc
     1381 agcaatcttt atcattcctt ttgaaggttt cagtaatcct aacgatgctt tcaccaaggt
     1441 tgacttggct gctccatttt ctccagttaa aatgacaaat tcacccgcat ctacatgata
     1501 agagacatct tctagtacag gttcatcatc atagtaaaag gtcaaatcag atatttcgat
     1561 ataacgcacc ttacaacctc cattctaaaa cttaattgat actttctttt aaagctttca
     1621 gatttttttc catgacagaa atataatttt cgcctgcttt gatttgttca ttagtcaagc
     1681 tttctaaagg attcaacact tctagcttca ctcctgtttc attcgacaat gtctcagcta
     1741 ccttcgaaga ggcattttct tcaaaataaa tcactttgat gttgtttttc ttcacaaatt
     1801 cttttagctc agctaagcga cttggtgtgg gttcctgatc tggtgaaacg cccgaaatcg
     1861 ctacttgttt taagtcgtat tgattcgcta aataagcaaa agcagcatgc tgagtgacaa
     1921 atgtgcggtt tgttgcatct tttagctcat cggagtattt ttgatccagt gcttcaagct
     1981 ttttgatata agcatcggca ttcttcgtaa aagtttcctt tttatctgga tatttttcgc
     2041 ctaattcttc agcaatcgtt ctgacttctt ttatagctaa taccggatcg agccaaacat
     2101 gtggatcgta ttcatgtgaa tgaccatgcc cttcttctga ttcttcatgt tcgtctgctt
     2161 cttgcgattc taaacggtcg attcctttag ctgcttcgat catcaaggtc tgtttgctat
     2221 ccactgaatc ctttaacgaa tccacaaaaa attccatgtc tgaactatta tacacgaaaa
     2281 catctgcatc ggaaatttct gctctttctt ttgcacttgg ttcgaaatca tgcggctccg
     2341 ttccagcagg gatcaaaagt ttcacgtttc cttcatcccc tacgatctct tttgtaaaat
     2401 catacatcgg ataaaaagtc gtaacgatcg tcaaatcctc ttttttgtct gcttccttgt
     2461 tcgtatttcc acatgctgca aacagtaaag cacctatcat taccgtagct gtcaaaagat
     2521 attttttcat ttatatgctc ctttcaatga acaaatcgga attttacgat ttattaacgt
     2581 gaactaatgt atcaaaataa aaaaacacag tcaatagaaa tccatcaatt tctgatttaa
     2641 gcatgtatat tcttgattaa atcgtaaaat ggatgagttg ctgctcatat ctagccattt
     2701 cactacaata aaaaactgaa gaataccatt ttaggattcc tcagtttttc atcacctatt
     2761 ttacttctta tattttattg cagggttcgt actaaataaa cttcctcaca aaaacctttt
     2821 aacccattat atactctctg cgctcttgaa taatgttgac acaaggcaaa aacggttggt
     2881 ccgcttccac tcatcaatgc agcatctgct ccgtacttga tcatacgctc tttgatttga
     2941 tggatgactg gatgccgctt tcccgttata tcttctaaac tgtttcccat ttttttgatc
     3001 atctgctgat aatcttgctc tcggatggct tcagcaagtc cgtggatgtc ttgatgttgg
     3061 atggcttgcg tatctacttc tctaaacact gtacgtgtag agacactcat tcttggtttg
     3121 accaacacaa cccagcactg tggcatagaa ggtaatactt ggatttcttc ccctttgcca
     3181 ccgacaaatg ccgtacttcc gtagacacag taaggaacat ctgtaccgac ttccaaaccg
     3241 atttctgcca gttcttctaa cgaacagccc agattccata attggttgat ccctcttaga
     3301 gcagctgcac agtctgtact tcctccgcct aatccagctg ctactggaat attcttctta
     3361 acaaagatct tgacaccggt tttgatattg tatttatttt ttagcaataa agcagcttga
     3421 taaacattgt tccgttcatc taccggtaaa aatgctttat ttgtttctac aatgattttg
     3481 tcttctgtta tttcttcaaa aaatagatga tctgccaagt ctacgctcga catcaccatt
     3541 tccaactcat gatacccatc ttgccgtcta tataatacat ccagtcctaa atttatcttg
     3601 gctggcgctt tttcaatcaa ttccatcctc tcacccattc gaaaagtcta agtcgcaagt
     3661 attctatcat agggaaaaat aaaagtatag cttatgtctg cctagtggtt gattttgtca
     3721 tcgtttcaac agctttcaca gaattttctc ttcataatcc aaacaagttc tagtgattct
     3781 tataattctg tgagcacttc ttctgccaaa ctcggaacat ttcctttttc ttttcctatc
     3841 agactgagta gctgcatggt agcaaatccg aaattacttc ctttcctata agcaaaataa
     3901 cgtccttccc aatgatcgac atattttaat ttcttttcat aagcagactg gaaggaatag
     3961 aaaaaattac cataattata tacgatatgc atgacttttt cttgaaagaa agaaaaatct
     4021 gtttctccaa cattagctaa tggagctaag cctagattgg cttccgagta gcccattttc
     4081 tcgtatacaa taaacagatt ggctaacaga tagtcagcta attcctcaga ttctttaggt
     4141 aagacccgca acaaatcata tcccgcctgc ttcttcgtca tcggctgagc ggtgatgaag
     4201 ccaacgactt tttgatcttt acggacaata ccgacaccgc tagataatac atatgattta
     4261 tcaaatcgtc ccacagagaa gtatttttct cgtgcacctt gaagccattt ttgtgaaact
     4321 tcatttacag cttgatgtaa ttcttccggc agttgatcgt accaagtaaa atggtatcct
     4381 tctttcatca aacgttgata ttcaagttgc tgactcagcc gagacaagtc ttctgttgct
     4441 agatcaacaa tccctgcttc acctactttc gtgaagtgaa aacccaattc atgaagcatc
     4501 actacgtagc tttctgatat cttataaaac gcaagctgat agcctaaaca atctgcttgt
     4561 ttcataaact gattcgttgc tgcccgcatc attttaggat tgccaactgg atctcctaaa
     4621 acaaagcatt tgtttccttt gatctgataa ccaaaaacga cttgatcttg gtcgtcttct
     4681 tggtaataaa aataactgta acctgacaag cgaagctgat ggcttgtagc tgtgcctccg
     4741 tatgtttcca ataaacggtc aaaacggtct ttttcgtact cactgccaag cttcgtagta
     4801 tcttcagcta aatattgata aagcgccact aaagctagca atgagatgcc caaaccaata
     4861 agccctgaga accacacatc atctgaagga aacaaaataa aatgatttcc tgttattcga
     4921 ccgactaaaa agcgtccgct gtgatagcct gcaattgcgt aaacgataaa caaaaagccg
     4981 aacaagctag catcaaagat tatagctccc caagaataaa cgaattttgt tcgataaaat
     5041 tcttttctgg ataaccacac agctaacaga atcaagacat ataccaatat caactgccaa
     5101 gataacgtac gtgtgattgt atttaaaata ccaaaagata gtaaagcaat cgtaggcaga
     5161 aatgcctttt tgacttttac tgaaagtgct ctagcaaggc cgagtaataa aaagccaacc
     5221 aatagattca ctgtctgatc taaaaaattg aatgagaacg gcaataatac ttgaaatagc
     5281 cgactgattg cagacagatt agtgattgtt gatagaagta ccatcatgat ccccgcaaaa
     5341 tagacagcac ttacgagcac gatatgcgca accttttgga aaaacagtcg cggaatgtta
     5401 tctaaaaatc gattgatttt gactcctgta tgtgtgatga acaatagaag tcccgtcata
     5461 aaaggtagaa cataataaaa caaccgataa tataatagcc aaactactgc tacatcttgc
     5521 cgaacagaca attgactcat tcctaagatc atcaaaacgt caaaagtccc cattccacct
     5581 gggaccatcg tcagcattcc tatcaatgta gcaatcatga acataggata gatcgaaagc
     5641 atcgaaatat ctattctcat caacttgccg atgatcaaaa ataccgcaag ggctcccgtc
     5701 cattgtccaa aggatgccag tagcagacca agcaagcctt ttggataaaa ctcgaagaac
     5761 aatgtctttc tctttagata agcaaaaaag aaaatgactg gtgctaaaaa actaccgccg
     5821 aaaagccaga tccaatagct tcgaaaaata ctttctgact gaataaaaaa gacatcaata
     5881 aatgtaagaa acgaccatac agacaaacca gtcagcataa atagtgcgac ttttgataca
     5941 gtggctaaaa ccatttttct atttgtctgc tttccataaa aactagcccg cagacttgct
     6001 cctaccacac caccaaaacc ggcgagattg ttgatcgtgt ttgttaccca cgcagcaaga
     6061 agaaactctt ttctttccat ttttggtttt ccttgttttt ctaagactct gatcgtcacc
     6121 caatcataca acaacatcgg agtgacaccc agcagtccag ccagaatcat gagcaggatc
     6181 gtcatgcggc tttgttgtcc catcctttgc caaatatctt gccaactcat gccattagca
     6241 atatttgcga cttgatttgc cacaaagact aacacggagc cgaaaaaaat cagttttaaa
     6301 atcagactat gttcctttat ccattgcagc atcaagtatc gtttattttt cacattttcc
     6361 cattcctaac ttgtatactt ttgtgtcata ccccaatgtt atcttattta ttttaatttt
     6421 tccactttag acctgttctt tgcttgcgat gatagaaaaa gaaagatatc ctaatgctaa
     6481 aggagaggat aacatgaaag taggatttat cggcacaggg aatatggcac aagctattat
     6541 tgatggttta ttaaaaaaag gaacagtctc gaaagaagag gtgttcgttt ccagtggaca
     6601 ttttgaaaat gcacaaaaat ttgctgctga aactggcgtc catgcatgca aaagtaatca
     6661 ggaagttgca tcacaatcag atatcgttct tttagccgtg aaaccaaaaa tcattgaaaa
     6721 agtattggaa gaactaagag aatatagcca gaaagtctta ttcatttcta ttgcttcagg
     6781 aaaatcatta atagatttag aaacagcgct tgaagatcct caggcctcca tcgtacgtgt
     6841 gatgccgaat gtcaatgtca gcgtaggagc aggcgtttct gcgatttgca aaaatgaagc
     6901 tgccacggag gaacagcttc agcaagctac gatgattttt gaagcaatcg gcagcgtcta
     6961 tcctttagca gaatcagatt tcagtacatt cattggcctt gctggcagct cacccgcatt
     7021 cgtttacatg ctgatcgatg cgatgggacg gactggtgta ctgaatggtt tgtccaaaca
     7081 gacagccact gagattgctg caaaagcaat tatgggttct tgtcaaaaat tcctttcaag
     7141 tgataaaaat ccatgggaac ttgtagacca agtttcttct cctggcggta caactgttgc
     7201 aggtgtagtt gccttagaag aagcaggatt catcccagca atcatcaaag gaatcaatac
     7261 gacgatcgag aaagatcttg agatgttgaa taaaaaataa tgatagcaat gttttttcgt
     7321 gccttacaaa aaaagaagag ctgtggcttt tgccgcggct cttcttttgt attttttatt
     7381 atttagcata atcagttgca cgagtttccc gaataacgat tactttgatg tgtccaggat
     7441 aatccagttc ttcttcgatt cgtttacgaa tatcacgtac taaacgaaca gcgtctaaat
     7501 ccgagatttc ttctggcttg accattaccc gaacttcgcg tccagcttga actgcgtaac
     7561 ttgattctac accggcgaag ctgttcgaaa tattttccag acttcttaaa cgacggatgt
     7621 agttttctaa tgattcactt cgcgcaccag gtcttgctgc tgagagcgca tctgctgcag
     7681 ctacaaggac agagataaca gaagtagctt ccacatctcc atgatgagaa gcgatcgcgt
     7741 tgattactac tgggttttct ttgtacttgg cagctaattc cgcaccgatc tcaacgtgag
     7801 atccttcaat ctcatggtca agtgctttcc caatatcatg gagcaacccg gcacgttttg
     7861 ctaattggat atcttcgcca agctctgctg ctaagatacc agctaatttt gcaacctcga
     7921 ccgaatgctt caagacgttt tgtccataac tagttctgaa tcgcagacgt ccgatgatct
     7981 tgattagatc tggatgaagt gtatgagcac cgacttcaaa cgctgcctct tccccgtatt
     8041 cgcgaatacg ttcatccatt tctttacgag atttctcgac catttcttcg atacgcgctg
     8101 gatggattcg tccatcttgt atcagttttt ccaaggtcat ccgtgcgatt tctcttcgga
     8161 ttggatcgaa tccggaaaga acaactgctt ccggtgtatc atcgatgatt aaatcgatac
     8221 cagtcagcgt ctcaagcgtt cgaatattac gaccttctcg tccaatgata cgacctttca
     8281 tttcatcatt cggaagggta acgactgaaa cagtcgtttc tgaaacttga tctgctgcgc
     8341 agcgttgaat cgctaatgat aataagtttt tcgctttgcg atctgcttct tctttggcac
     8401 gctgttcaga ttcttttacc attagagtta actcatgatt cagctcttct tcagtcgatt
     8461 tcatgatgac atctcgtgcg tcctcttttg ataaaccagc aattttttct agttcttttt
     8521 gttgatcttc aatcattttt tcaacgtctt tttctcgctc ttcaattaat tgctgttttg
     8581 aactaagtgc ttcttcttta ctttcaagtg agttctcgcg tttttctaac gagtcatctt
     8641 tacgattcag cgtttgttcg cgttgaatca aacgattctc ttgagatttt aattctaact
     8701 tactttctct cagctcactt tcaacttcag aacggtattt ctgattttct tccttagctt
     8761 ctaacaatgc ttcttttttt agagtttctg cttctttttt tgcactatcg atgattccag
     8821 cagctgatga ttgtgcacca gctatttctt tctcgtgacg tgactttgcg accataaacc
     8881 ctaaaccaag accgacaatt aaaccgatga tagcgaggag aatgttgaat accataaaat
     8941 ccacctccat actatctttt tcatttttat catctataca gcttctatga taaattattc
     9001 taaactcgtc aatatgcatc ttcgcatata tgtgtacaaa aaacatgcac aactttattc
     9061 taatgtttgt accccgtagt gtcaacttta aataaacaaa gaaacggctt tctacttatt
     9121 cattaaacga tggtttttaa aataaatgac actagtttca tataggtaaa cctttaaatt
     9181 gaga
//
LOCUS       DAZKCY010000097         9083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000097 DAZKCY010000000
VERSION     DAZKCY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.098"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..279)
                     /locus_tag="ACG8XC_002139"
     CDS             complement(<1..279)
                     /locus_tag="ACG8XC_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9191814.1"
                     /translation="MSITKNIKCTDEKTSHSIKQILNIKDSHITFSKDAISKERVHGR
                     MANVFTGLLTGEAHRCECCGFDSVIRHAYHDSWIQLLPYQEVPTYLHLY"
     gene            complement(394..624)
                     /locus_tag="ACG8XC_002140"
     CDS             complement(394..624)
                     /locus_tag="ACG8XC_002140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191815.1"
                     /translation="MIEINEKILVFDKKKDDKDNDSYDDFNSGTLILDATCAPQNIKY
                     PTDTELMNDTRTHAEKIIDVVCEGKKLKKPVL"
     gene            complement(714..803)
                     /locus_tag="ACG8XC_002141"
     CDS             complement(714..803)
                     /locus_tag="ACG8XC_002141"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191816.1"
                     /translation="MTFGALLIQKEYSYSDEETVLQIQENPYL"
     gene            complement(800..985)
                     /locus_tag="ACG8XC_002142"
     CDS             complement(800..985)
                     /locus_tag="ACG8XC_002142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191817.1"
                     /translation="MYKLQTTVQLAFEDFNQPIGLKMNPSNRWIQKAELIPWAELEKD
                     YAKNFRKKKETSQNPFA"
     gene            1154..3265
                     /locus_tag="ACG8XC_002143"
     CDS             1154..3265
                     /locus_tag="ACG8XC_002143"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9191818.1"
                     /translation="MNTKDLKLLETIVRSHVTNSQQLKSLVHFSTRQITYSIKKINDE
                     LSLHGLSLIKRSNSGEWLYEANDLQKLLIYMNQAMVFKEGAIPEYINDDLAYSEIRRY
                     SELLFILANPKKVTTQEIMAFIGISKNTARNDFSKIKKELPPDYTLIFDPQNGYMIES
                     SMAKLHTLLLEVFNYLFERHATVNRFESLFSEEREFVGFFIHTYEQTFKLKFSDNSLT
                     KLYYLIGLSLSSFKRRTKGQIDFSYDLQELDEFQMIRSCIDQHYQHAEIAIDYEWLTL
                     LFLSFNTISNEFEILDSRIYQVIMEMITLFQSKSAIRISNKEELTNRLMNHLRPAIYR
                     IRYELPFEIDDLAEIVKENHEVSAVLNFVKESIVPLEAFLRKTIPETELYLISVYFCS
                     ELLNSSIIETPVRKKAIVVCSNGLIMANVMAKTLKEIFPDIQFVASSSVREMDKFVED
                     VDLIFSNQPLKSEIPLYIIQPIMNEEEKNRLRNRVLIDFSTNNISKETRNLTEIIKRY
                     SAVQNEESLQTDIERFLINYANSLIYLDRQSKQAQKISFVELLVSEGIILVSTKQLTW
                     QNAMELSCQPLLKEQVINQNYQTILMSQMADEEAYYFLNNRIAIPHSSPENGVIKGGC
                     SLLISVDPIIFPGNRPIHFISPFAIGKKENYLLAINQLLQLSKDEEYQQEILLCTTED
                     EVRKKIKNFLRRKQYVNNEFS"
     gene            3243..3893
                     /locus_tag="ACG8XC_002144"
     CDS             3243..3893
                     /locus_tag="ACG8XC_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9191819.1"
                     /translation="MSITNFPELTIILRGYSFEEAMFVMEELAAMNTSVGIEVTTNND
                     DFARIITEGNKKFGQQLLIGAGTVLTFSQADQAIAGGAKFLLGPKKFSTPIFSLAKER
                     GVVTVPSAFTPSEIQVMFDEGADIVKVFPAVTVGPTYFKQLAGPFGIRPLMAVGGVTI
                     DNIAAFKKAGVVYFGVGSSMFQKADIQNRNGQAIRKSIQSYLDKLAEAGSEEDESH"
     gene            3880..4332
                     /locus_tag="ACG8XC_002145"
     CDS             3880..4332
                     /locus_tag="ACG8XC_002145"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9191820.1"
                     /translation="MSLIKLEEKNILLNQSFSNQEEYFQKITKKLMTENMVKEGYLES
                     ITKRETTYPTGLYIGERSIAIPHTDYQLSNVTAILVSVLKDSLHFQRMDDPEEMVSVN
                     VIIHLLFDSPDKQPQLLKELMSKVTNQDFLQAIMEAKSKTEVLTAFNQ"
     gene            4349..4654
                     /locus_tag="ACG8XC_002146"
     CDS             4349..4654
                     /locus_tag="ACG8XC_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HFP9191821.1"
                     /translation="MSKKTIIVACASSMITSTMAANKIRKIAKTNGLPEPRILQLKFS
                     EVAGALESNQVDFIVPTGKLQENVTKGIKVIPGTPFVTGVNEEKSESLVLEALKELD"
     gene            4666..5943
                     /locus_tag="ACG8XC_002147"
     CDS             4666..5943
                     /locus_tag="ACG8XC_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9191822.1"
                     /translation="MNIVIDALNWISSLGPMIMMPIIILILGLFFRIKLNTLLKSALT
                     VGIGFAGVNIIINWFISQVGPSVQLMVKNWGIQTSIMDVGWPARAAATWSFPLAAVMV
                     FIILGINVLMLIFKKTNTVMVDFWSYNHYIFVAALVYYATDKNIILAVIFGAIDAIIS
                     FKLADWTTPLVEDYFELEGVNFPTANSVGWAPLAWLLNKLWDRIPGLNKVDARPEKIQ
                     ERFGFVGEPMFMGFVIGLVIGLLAKEPVDKVLILGMSTSATMVLTPKMMQILMEGLLP
                     FANSVKKFLNVKFPGNNFTMGVDAALTVANSSAIATGIIMVPITLVLAAILPGNKLLP
                     ISDIAYQAMWLSAWPVAFSKGNIIRGILSTTIITACVLLIATSLAGIHTQLALAGGFQ
                     LPAGMTQISTEDAGTHIIGFILMKIAEFISNIF"
     gene            5962..7110
                     /gene="dgoD"
                     /locus_tag="ACG8XC_002148"
     CDS             5962..7110
                     /gene="dgoD"
                     /locus_tag="ACG8XC_002148"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFP9191823.1"
                     /translation="MKISKITVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRFLVGQNPFAIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKALNVPV
                     YELLGGAARDKIKVYSWIGGDRPSEVVEQAQDRVDRGFTAVKMNATEELHYIDSYEKV
                     AEVVERVASIRERFGDTLSIGVDFHGRVHKPMAKVLAKALEPYHPMFLEEVVLPENEE
                     HFSEVANAVAVPLATGERLYTRWQFKSILQQGAIDIIQPDVALCGGILETRKIAAMAE
                     AYDIAVAPHAPYGPIALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEIFQ
                     YKDGFVDLPTKPGLGLEMDEDRVKEIAQEGLVWTNPKWKNYDGTIAEW"
     gene            7122..8024
                     /locus_tag="ACG8XC_002149"
     CDS             7122..8024
                     /locus_tag="ACG8XC_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HFP9191824.1"
                     /translation="MINTVTGPIEAESLGTTFIHEHLYVIPNELRKYEDYTLDDIYHS
                     IAEVMAFKQAGGKTIVDLTPINYGRNPLALKMISEATGVNVAFVTGFHKEEFQPKWLT
                     TLNDDEIFELLYSEITHGVTSQNLLPGAMKVGTSKNEVTLAEQRILAIAGQVQKKTGI
                     PIITHCDGGTMGLEQINCLTKNGAKASNICLSHVDLTKDFHYLAQICETGASICMDHI
                     GRELANHDADRVQLIKRLVDAGYENQICLSGDMGRKKYLPTYGGTPGLTYILTSLKEE
                     LLKVMSEPTFNKMIEMNPINILSK"
     gene            complement(8693..>9083)
                     /locus_tag="ACG8XC_002150"
     CDS             complement(8693..>9083)
                     /locus_tag="ACG8XC_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9191825.1"
                     /translation="FKQFPLFQGKYMTESEVLDYLLSIDNQLRQSYEVYQELLAAFDA
                     KDFTEFFNLIESLPHSLNDGFKKAICYLKKHTEAITNSLKYPYSNGKLEGKNNLIKVI
                     KRIAFGFRTFRHLRMRVLIQQNICEII"
BASE COUNT         2948 a         1397 c         1854 g         2884 t
ORIGIN      
        1 gtacaggtgt agataagttg gtacttcttg atacggaagc aactgaatcc aactatcatg
       61 ataagcatga cggatgacag aatcaaaccc acaacattcg caacgatgcg cttctccagt
      121 taataagccg gtaaatacat tggccatacg gccatgaacg cgctctttac taatggcatc
      181 ttttgaaaag gtaatatggg aatctttgat gttcaaaatt tgtttgatgg aatgacttgt
      241 cttttcatcc gtacatttga tattcttagt tatggacatt tggttatcct cctatgattg
      301 tttttgccga tttaattata ggacaaccag atgtcttttt gttataaaaa aatatttggc
      361 gtcactactc agtttgagta acaaaaccaa atattataga accggctttt tcagtttttt
      421 cccttcacaa acaacatcga tgattttctc ggcatgggta cgggtatcat tcattaattc
      481 tgtatcggtt ggatatttga tgttttgtgg agcacaagtg gcatccaaaa tcaatgttcc
      541 ggaattgaaa tcgtcataag aatcgttgtc cttatcgtct tttttcttgt cgaagacgag
      601 tattttttca ttgatttcta tcaacgttgt ttcgtccaag cgtttccgga aatagaccat
      661 tgtagagcat cgaaaggctt ggcatcttga tagcccggca aaccgatgaa gaactataaa
      721 tacgggtttt cttgaatttg taagaccgtt tcttcgtcgc tatagctata ttccttttga
      781 atcagtagag ctccgaaggt catgcgaaag ggttttgcga cgtttccttt tttttacgaa
      841 aatttttagc gtagtctttt tcaagttcag cccatggaat gagctctgct ttttgaatcc
      901 aacggttaga cggattcatt ttgagaccta tcggttgatt gaaatcttca aaagccaatt
      961 gaacggttgt ttgtaattta tacatgaaaa gcctccaaaa ttgagtggta ctccacacca
     1021 ttctgatggt gcataagata aaaaattaaa gaagtgcctg tcatttgtct gatttaagcc
     1081 aaggtttgac aaattttgga tattccaatt aatactgtaa gcggttatat ttaactcagg
     1141 gaggtagatg aatgtgaata caaaagattt aaaactgtta gaaaccattg tacgatcaca
     1201 tgtgacaaac tctcaacaat tgaaatcatt agtacatttt tcgactaggc agataactta
     1261 ctctataaaa aaaattaatg atgaattatc ccttcatgga ttgagtctga taaagcgaag
     1321 taactcaggg gagtggttat atgaagccaa tgatcttcaa aaacttttga tatacatgaa
     1381 tcaagcaatg gtttttaaag aaggtgcgat tcctgaatat ataaacgatg atttggctta
     1441 tagtgagata agaagatatt cggaactgtt atttattcta gccaacccca aaaaagtgac
     1501 aacccaagaa ataatggcgt ttattggtat cagtaaaaat acggctagaa acgacttttc
     1561 taaaataaaa aaagagttgc caccagacta cactttgata tttgatcccc aaaatggcta
     1621 tatgattgaa tcaagcatgg caaagcttca tacattgctc ttagaagttt tcaattattt
     1681 atttgaaagg catgcaacag ttaaccgttt tgagtcactc ttttctgaag aaagggaatt
     1741 tgttggtttt tttattcaca cctacgaaca aacttttaaa ttgaaatttt ctgataattc
     1801 actaaccaaa ctttattatt tgataggttt atcactttct agttttaaaa gaaggacgaa
     1861 aggacagatt gattttagtt atgatttaca ggaattagat gagtttcaaa tgattcgaag
     1921 ctgcattgat caacattatc aacatgctga gatagctata gattatgaat ggttgacgct
     1981 tctgttttta tcattcaata caatttcaaa tgagtttgaa attttagatt caagaatcta
     2041 tcaagtaatt atggagatga ttaccttatt tcaaagtaaa tcagctattc ggattagtaa
     2101 taaagaagag ttgaccaatc gattgatgaa tcatttacga cctgcaatct atcgaattcg
     2161 ctacgaatta ccatttgaaa tagatgattt agctgaaatt gttaaggaaa accatgaagt
     2221 tagtgcggta cttaattttg ttaaagaatc cattgtacct ttggaagcat ttttgcgtaa
     2281 gacgattcct gaaactgaat tatatttgat ttctgtttat ttttgttcgg agctattaaa
     2341 ttcttctatt atcgaaacac ctgttagaaa aaaggcaatc gtcgtatgta gcaatggatt
     2401 gattatggcc aatgtaatgg cgaaaacatt gaaagaaatt tttccagata ttcagtttgt
     2461 tgctagttct tcggtcagag agatggataa gtttgtcgaa gatgttgatt tgatttttag
     2521 caatcaacca ttgaaaagtg aaattccatt atatattata caaccgatta tgaacgaaga
     2581 agaaaaaaac cgcttgcgga accgtgtact cattgatttt tctacgaata atatcagcaa
     2641 agagactagg aaccttactg aaattattaa gcgttactct gctgttcaaa atgaagaaag
     2701 tctacaaact gatattgaga gatttttaat taattatgca aattcactaa tatatctaga
     2761 tcgtcaaagt aaacaggcac aaaagatatc ttttgtggaa ttgcttgttt ctgaagggat
     2821 tattcttgta tctacgaaac aacttacttg gcagaacgcg atggaattgt cttgtcagcc
     2881 tttattaaag gagcaggtaa ttaatcagaa ctatcagact attttaatga gtcaaatggc
     2941 ggatgaagag gcttattatt ttcttaataa tcgtattgct attccacatt catcgccaga
     3001 aaatggggta attaagggcg gttgctcgtt acttatatca gttgatccaa ttatttttcc
     3061 tggaaatagg cctatccatt ttatttcccc ttttgcaatt ggaaagaagg aaaattattt
     3121 acttgcgatt aaccaattac tacaactttc taaggacgag gaatatcagc aagaaatact
     3181 tctttgcacg acggaagatg aagtgagaaa aaaaataaaa aattttttaa ggaggaagca
     3241 atatgtcaat aacgaatttt cctgaattaa ccatcatttt aagaggatat agttttgaag
     3301 aggcgatgtt tgtcatggaa gaattggctg cgatgaacac ttcggtagga attgaagtaa
     3361 caaccaataa tgatgacttt gcacgtatca tcacagaagg caacaaaaaa tttgggcaac
     3421 aattattgat tggtgcaggg acagttttaa cattttcaca agcagatcaa gcaattgctg
     3481 gtggtgcgaa atttttatta ggaccaaaaa aattttcaac accaattttt tcgttggcta
     3541 aagaacgtgg ggtggtaact gtgccatcag cttttacgcc atcggaaatt caagttatgt
     3601 ttgatgaagg tgccgatatt gtaaaggttt ttccagcagt cactgtaggg ccaacctact
     3661 ttaaacaact ggccggtcct tttggtatac ggcctttaat ggcagttgga ggagtgacga
     3721 ttgataatat tgcggctttt aaaaaagcag gagttgtgta ctttggggta ggatcaagca
     3781 tgtttcaaaa agctgatata caaaaccgca atgggcaagc tattcgaaaa tcaattcaat
     3841 catatttaga taaactagct gaagcaggaa gtgaagaaga tgagtctcat taaattagaa
     3901 gaaaaaaata ttttgttaaa tcaaagtttt tcaaaccaag aagaatactt tcaaaagatt
     3961 acaaagaaac tgatgacgga aaacatggta aaggagggat atctcgagtc aatcactaag
     4021 agagaaacaa cttatccaac ggggctatat attggggaac gctcaatcgc aattccacat
     4081 acagattatc agttatcaaa tgttaccgct attttagtat ccgttcttaa agatagtttg
     4141 cattttcagc gaatggatga tcccgaagaa atggtttcag tgaatgttat tattcacctt
     4201 ttattcgata gtcccgataa gcaaccgcaa ttattgaagg agttgatgag taaagttacc
     4261 aatcaggatt tcttacaagc aattatggaa gcaaagagta aaacagaagt tttaactgca
     4321 tttaatcaat aaattggagg gaaatacaat gagtaaaaaa acaattatcg tcgcatgtgc
     4381 atcatcaatg attacatcta caatggcagc taataaaatt cggaaaatag caaaaacaaa
     4441 tggattacca gaacctcgaa ttttgcaact aaaattttca gaagttgcag gtgctttaga
     4501 atcaaatcaa gttgacttta tcgttcctac agggaaatta caagaaaatg taaccaaagg
     4561 aattaaagtt atacctggaa caccatttgt tactggagtg aatgaagaaa aatctgaatc
     4621 tttggtgtta gaagcattga aagagcttga ctaggagggt gaaaaatgaa tattgtcatt
     4681 gatgcgttaa actggatctc aagtttgggt ccgatgatta tgatgcctat cattatcttg
     4741 attttaggat tattttttag aattaagcta aatacattac taaaatcagc gttgactgtg
     4801 gggattggtt ttgcgggtgt aaatattatt attaattggt ttatttcaca ggtaggtcca
     4861 tctgttcagt taatggtcaa aaattggggt attcaaacaa gtattatgga tgtcggctgg
     4921 ccagctcgtg cagcagccac ttggtctttt cctctagcag cagttatggt atttattatt
     4981 ttgggtatta atgtgttgat gctgattttt aaaaagacca atacagtaat ggtcgatttc
     5041 tggagttata atcactatat ttttgtagct gctttggttt attatgcaac ggataaaaat
     5101 attatattgg cggtaatctt cggggcgatt gatgcaatta ttagttttaa actcgctgat
     5161 tggacaacgc cgttagtaga ggattacttt gaactagaag gggttaattt cccgactgcg
     5221 aactcagttg gttgggcacc cttggcttgg ctacttaaca aattgtggga ccgcattcct
     5281 ggattaaata aggtagatgc tcgtcctgaa aaaattcaag agcggtttgg ttttgttggt
     5341 gagcctatgt ttatgggatt tgtcatcggc ttggttattg ggttattagc aaaagaacct
     5401 gttgataagg tattgatatt gggaatgagt acttctgcaa caatggttct aacacctaaa
     5461 atgatgcaaa ttttaatgga aggattatta ccctttgcta attctgttaa gaaattcttg
     5521 aatgtaaaat ttccaggaaa caattttaca atgggtgtgg atgctgcgtt gacagttgct
     5581 aattcaagtg ccattgctac gggtattatc atggttccga ttaccttagt gttggctgct
     5641 attttaccag gtaacaaact cttacctatt tcagatattg cctaccaagc aatgtggcta
     5701 tctgcatggc cggtcgcctt ttcaaaagga aatattattc gcggaatact ttccaccacg
     5761 attataacag cttgtgtgct tttaattgct actagtttag ccggcataca tacacaatta
     5821 gctttggctg ggggattcca attaccagca ggaatgacac aaatatctac cgaagatgca
     5881 ggtacacaca ttattggttt cattttaatg aagattgctg agtttatttc aaatattttc
     5941 taatattcag gagggcatac agtgaaaatt tcaaaaatta cagtctataa agtaaaacca
     6001 agatggattt ttgtcaaaat atctacagat gaaggtattg aaggctgggg tgaaatgatt
     6061 tctggaacta agactgaaac agttgtggca ggagcttatg agataggaaa tcgattctta
     6121 gttggtcaaa acccatttgc aattgaacgt ctgtttcagg aaatgcatcg ttcatttttc
     6181 cgaggtggtc ctattaatgg aacgattgtc tctggacttg aaatggcttt atgggatatt
     6241 aaagggaaag ccttaaatgt tccagtttat gaattactag gtggagcagc ccgtgataaa
     6301 atcaaagtat attcttggat tggtggagat cgtccttctg aagtagtaga gcaggcacaa
     6361 gatcgagtag atcgtgggtt taccgccgtg aaaatgaacg cgacagaaga attacactac
     6421 attgattctt atgaaaaagt agctgaagta gtggaacgtg ttgcttcaat tagagaacgt
     6481 tttggagata cattgagcat tggtgtcgat tttcatggtc gtgttcacaa accaatggct
     6541 aaggttttag caaaagcact ggaaccctat catccaatgt tcttagaaga agtggttttg
     6601 ccggagaatg aagaacattt cagtgaagta gccaatgcag ttgctgtacc actagcaacg
     6661 ggtgaacgtt tgtatacaag atggcaattt aaatcaattc ttcaacaggg agcgattgat
     6721 atcattcaac cagatgtagc tttatgtgga gggattttag aaacaagaaa aattgctgca
     6781 atggctgaag cttatgatat agcagttgcc ccccatgcac catatggacc gattgcgtta
     6841 gcagctacat tacaagttga ttcttgtaca cctaatgtgt ttattcaaga acaaagctta
     6901 ggtattcatt acaacaaagg gtttgatttg ttagattttg taaagaataa agagatattc
     6961 caatacaaag atggcttcgt agatcttccg acaaaaccag gtttagggtt agaaatggac
     7021 gaagatcgag tgaaagaaat tgcacaagaa ggtttagttt ggaccaatcc taagtggaaa
     7081 aattatgatg gtacaatcgc tgaatggtag gagatgagtg catgatcaat acggttacag
     7141 gcccaatcga agctgagtct ttgggtacaa catttattca tgaacattta tatgtcattc
     7201 ctaatgagtt aagaaagtat gaagattata ccttggatga tatttatcat agtattgcgg
     7261 aagttatggc ttttaaacaa gctggtggaa aaactatcgt tgatttgaca ccaattaatt
     7321 atggacgaaa tccgttagct ttgaaaatga tatctgaagc taccggagtg aatgtagcat
     7381 ttgttacggg attccataag gaagagtttc agccaaaatg gttgacaacg ctcaatgatg
     7441 atgagatctt tgagttgctt tattccgaaa taactcacgg agtcacttca caaaaccttt
     7501 tgccaggagc aatgaaagta ggtacgagta aaaatgaggt tacattggct gaacaacgaa
     7561 tactagccat agccggtcaa gtacaaaaaa aaacagggat tcccattatt acgcattgtg
     7621 atggggggac gatgggactg gagcaaatta attgtttaac aaaaaacggt gcgaaagcaa
     7681 gtaatatttg tttgagtcat gtagatttaa ctaaagattt tcactattta gctcaaatct
     7741 gtgaaacggg agcaagtatt tgtatggatc atattggccg agagttggcg aatcatgatg
     7801 cagaccgcgt ccaactaatt aaacgactgg ttgatgcggg ttatgaaaat cagatttgtc
     7861 tttcaggtga tatgggacga aaaaaatatt tgccaactta tggaggaacg cctgggctta
     7921 cctatatttt aacaagtttg aaagaagaac tattaaaagt aatgagtgaa ccaactttta
     7981 ataaaatgat tgagatgaat ccaattaata tactgagtaa gtgaagtcaa ttgaatcatt
     8041 cagaagaact aatgaaatta gttgctttcg cagggttaat tcttctctct gggtttggaa
     8101 aagagggctt agcttcggat tccctagtta aactaggggt tttatcaggt tattgtactg
     8161 attataagta cctgaattat tcatgaaata ctttttccgt cgttttatag gcagttgggt
     8221 caataaaaag atcagaaaaa cgaacgagag actctcataa cgtgccacta aataaaaatc
     8281 gtacgttatt ttaaaaaatg ggttgattct aggcagtact ttgtcggtct atttttagaa
     8341 aaagaggttc tttgtcaaac atacatgaat tcgcaagctc attcaccctg aaggatgaaa
     8401 ggcagaaact gtagtatact aatggtggaa aggaaaaaaa gccgccagca atttggaaaa
     8461 gaacgttatg tcgattgtca aactggcttg ctttatttct cgagaaccct gaaatatagc
     8521 caatgagcat gtgtataaaa aattgatttt atatgaaata aagcactcct ttcctaaaat
     8581 tattggaaag gagtgcgttt tttatggaaa ttatttaggt tctttgtcaa ttgctattgt
     8641 tttgctcaaa tcgtatgtta ggaaatgctt cggcatttcc ttttttgcgt tattaaatga
     8701 tttcacagat gttctgttga atcaggacac gcatccggag atgacgaaaa gtccgaaatc
     8761 caaaagcaat tcttttgatt actttgatga ggttattctt tccctccaat tttccgtttg
     8821 aatagggata ttttaaggag tttgtgattg cttctgtgtg ctttttcaga taacaaattg
     8881 cctttttaaa cccatcattc aaggaatgtg gcaatgattc aatgagattg aaaaattcag
     8941 taaaatcttt tgcatcaaaa gcagctaata actcttgata gacttcatag ctttgtcgta
     9001 gttggttatc tatagagagt aggtaatcaa gcacctccga ctccgtcatg tatttccctt
     9061 ggaagagggg aaattgctta aaa
//
LOCUS       DAZKCY010000098         9018 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000098 DAZKCY010000000
VERSION     DAZKCY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.099"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACG8XC_002151"
     CDS             <1..1101
                     /locus_tag="ACG8XC_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9191826.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACG8XC_002152"
     CDS             complement(1280..1615)
                     /locus_tag="ACG8XC_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9191827.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACG8XC_002153"
     CDS             complement(1599..1862)
                     /locus_tag="ACG8XC_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFP9191828.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACG8XC_002154"
     CDS             complement(2322..3125)
                     /locus_tag="ACG8XC_002154"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9191829.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACG8XC_002155"
     CDS             complement(3103..4227)
                     /locus_tag="ACG8XC_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9191830.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACG8XC_002156"
     CDS             complement(4243..5058)
                     /locus_tag="ACG8XC_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9191831.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACG8XC_002157"
     CDS             complement(5048..5920)
                     /locus_tag="ACG8XC_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9191832.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACG8XC_002158"
     CDS             complement(6005..7294)
                     /locus_tag="ACG8XC_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9191833.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7950)
                     /locus_tag="ACG8XC_002159"
     CDS             complement(7438..7950)
                     /locus_tag="ACG8XC_002159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191834.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACG8XC_002160"
     CDS             complement(8017..8160)
                     /locus_tag="ACG8XC_002160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9191835.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(8643..>9018)
                     /locus_tag="ACG8XC_002161"
     CDS             complement(8643..>9018)
                     /locus_tag="ACG8XC_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9191836.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2778 a         1933 c         1427 g         2880 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacagccaga ctgtagacaa aagctgttcc
     8701 aaactcgtaa ctcgagcttg gaacagcttt ttatctactt cgtttcgaat ttttgaacaa
     8761 aaaaataaag gctccttgcc tttccatagc caggtggcta atttctttaa attcattgca
     8821 gcaaacacta gcatcgtatg cgtcgcgact ttttccaagc ctcgatactt ggtccaacgc
     8881 atcccatgct tttcttttgc atccgcaaat attcgttcaa tcgtttgttt ccgttttttg
     8941 taaattgatt tattcagatc tgttaggcga agatgttcca cttcatccat cagatctccc
     9001 caaacatgtc tcgtgatg
//
LOCUS       DAZKCY010000099         8997 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000099 DAZKCY010000000
VERSION     DAZKCY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.100"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..739
                     /locus_tag="ACG8XC_002162"
     CDS             <1..739
                     /locus_tag="ACG8XC_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFP9191837.1"
                     /translation="SLLIKTLERKSSSNYYEGGNEIVSKMKISEVTLDETDDFTYYKG
                     YKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGDNVLVDTILVPLPEN
                     YEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHGYLIEILKKYEITVP
                     RDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRHSLAHPNTN
                     IIGKREAKLIIGISSTVTDYISGYLDK"
     gene            800..1300
                     /locus_tag="ACG8XC_002163"
     CDS             800..1300
                     /locus_tag="ACG8XC_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9191838.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            1356..1994
                     /locus_tag="ACG8XC_002164"
     CDS             1356..1994
                     /locus_tag="ACG8XC_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFP9191839.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            2066..2461
                     /locus_tag="ACG8XC_002165"
     CDS             2066..2461
                     /locus_tag="ACG8XC_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9191840.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2445..4898
                     /locus_tag="ACG8XC_002166"
     CDS             2445..4898
                     /locus_tag="ACG8XC_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9191841.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4895..6922
                     /locus_tag="ACG8XC_002167"
     CDS             4895..6922
                     /locus_tag="ACG8XC_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP9191842.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6919..7941
                     /locus_tag="ACG8XC_002168"
     CDS             6919..7941
                     /locus_tag="ACG8XC_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFP9191843.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7958..8887
                     /locus_tag="ACG8XC_002169"
     CDS             7958..8887
                     /locus_tag="ACG8XC_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9191844.1"
                     /translation="MIIKIERKEKKERPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT         3063 a         1506 c         1789 g         2639 t
ORIGIN      
        1 atctctttta attaaaacat tagaaagaaa gagctcttca aattattatg aaggaggtaa
       61 cgagattgtt tctaaaatga aaatatcaga ggtaacttta gatgagactg acgattttac
      121 ttattataaa ggatataaaa agggctggaa tacgctttgc acatatctta aaataagagt
      181 tccttttgat gatttagact tttttgaaag tcataaagat ttgattaccc agacagcaag
      241 ctccatatat gataaacaag gtgataatgt attagtggat actattttag taccacttcc
      301 agagaattac gaagtaatta actttagtca gttgaaaatt agtgatgtgg tttcacaagc
      361 tattgaagac gctgagtctt ttatgagtaa tggtgaatat caacgtgcct ttgacagagt
      421 tcacactgct tttcatggat atttaattga aatactaaaa aaatatgaaa ttacagttcc
      481 tagagacgaa aatctatcta aattatatag taggatacaa caattaattg aaaaagaaat
      541 tcaacctact gaacttgctg atatagtgaa gacaacgata aggtcatcaa atggaatgat
      601 tagctctctt aatgaagcaa gaaataggca ttcacttgca caccctaata caaatattat
      661 tggaaagaga gaagcaaaac taataatagg tatttcaagt acagttacag attatattag
      721 cggatacctt gataagtagc gtcattaact gattgctact ttttttatga ctatttttat
      781 aaaaaggatg tgataagaat tggaaatgaa agtctatgtg gcaaatttag gacggtataa
      841 tgaaggtgaa ttagtcggag catggtttac tccaccaatt gatgaggaag aaatggcaga
      901 aagaatcggt ttaaatgagg actacgaaga atatgcaata catgattttg agcttccttt
      961 tgatgtggac gaatacacgc cgattagtga aatcaaccgt ctatgtgaag ccattcaaga
     1021 aatcgaaggt acacctattt ataatgaact caaagaaatt caaggcatgt ggtttagtag
     1081 cttggaagaa ttactggaaa acaaagagga tattcattgt tatagtgatt gtgattcgat
     1141 ggaagatgtc gcccgttatt acgtggaaga aacaggacag cttggtgaag tcccttccaa
     1201 tttacaaaat tatattgact accaatcttt gggacgtgat atggaaatag agggtaatta
     1261 cctagttacc tctcatgggg tatttgaata ttgtcagtaa gtttaacaat ccgtgttata
     1321 attaaattaa taaatagcac ggagaggtat aaattatggt aaatgtattt gaaaatctta
     1381 aactattaaa actagacatg aaagataaag gctgggtgat agattccttt tactttcgtt
     1441 acaaacagca aaattatatt gtacttgtta agctatttga aaaagaggaa aaagttccag
     1501 aatatgctct gttaaaacta gaatttttaa aggaaaacga tttttcagat atgctagctg
     1561 tctatgctaa ttcggtaaaa ttatatacag acacgaaaac aataagagaa tattttggta
     1621 tcgaatacag tagtaacttg ggagatgttt tatttcaatt tagccaaact cttgctcgat
     1681 ttattccgac agaagttagt gagaagaaaa atgaagacca aaaagaagcc atgtgtttct
     1741 cattaagtca aagtgattca gaagatccca gaaaaaaata ttgtttttca gttagaagaa
     1801 atcctttaaa gggaaatggt gagctgggaa aacgaagtcc ttttaatgac aataaaacga
     1861 gattgtatag acccagctta tatgaacgat tagggtcaga taccaattta agtttcagat
     1921 attcaatgaa tccagatgat gaagaaactg atgaaggtat tattgcaaaa tggacaaaaa
     1981 ataaaattga atagttaaag cattgaaaga gcaatcacct attttgattg ttctttttct
     2041 atttttacgg aaaggacgtg aagacatgaa aaaaataaga agttacacca gtatttggtc
     2101 agtagaaaaa gtgatttatg cgattaatga tttacaactc cctttcccaa ttacctttac
     2161 gcaaatgaca tggtttatcg tttctttgtt tgtcgtcata ctctttggca gtttaccacc
     2221 actttctctg attgacggag cactactcaa atacttaggg attcccattg ttctcactgg
     2281 gttcatgtgc caaaagacct ttgacggaaa gaaaccctat ggatttctaa agtctatcat
     2341 tacctatgct ctacgcccta aagtaaccta cgctggcaaa caaatacgat taaaaaaaga
     2401 aacaatgaat gaatcgatta cgattgtaag gagtgaacaa ctagatggca tatccgatta
     2461 aatatatgga agataatctc gtatttaatc atgatggcga agtattcgct tattatgaat
     2521 taattcccta taattacagt tttttaagtt ccgatgaaaa aattcaagtt catgataatt
     2581 tctgtcaatt gattgcccaa aatcgtgatg gcaagattca tgctctgcaa ctagcaaccg
     2641 aatcaagtat tcgagccaca caggaacaat cgaaaaaaga aatcactgga cgattaaaag
     2701 agatagctta cgaaaaaata gatgaacaga ccgaagcgtt aatttctatg attggtgaca
     2761 atcaagtaga ctatcgcttt tttattggtt ttaaactcta cctcaatgaa caggaagtat
     2821 ccgtaaaaaa tgttgggaaa gaaattcaaa aagcatttgc ggaattcttt catggtgtga
     2881 atcacaaatt aatgggcgat tttgtttcta tgcctatggc ggaaatcgaa cgcttttcta
     2941 agatggaaca gttgttagaa agtaaattat ccagacgctt tcagattcgt aaattggaga
     3001 aagatgattt tggttatatt attgaacatc tttacgggca aacaggtgtc ccttttgatg
     3061 actacgacta ccatttaccg aaacgatacg aagataaaaa agtgttggta aagaaatacg
     3121 atattctcaa acccactcgc tgtttaatcg aagaaaatca acgctattta cggattgaac
     3181 aggaagatac cactacttat gtggcgtatt ttacaattaa tagtattgtt ggtgaattgg
     3241 atttccctaa tagtgaaatt ttttattacc agcaacagca gtttactttc cccgttgata
     3301 ccagtatgaa tgttgaaatt gtcgccaata aaaaagcctt atctaccgtt cgcaataaaa
     3361 agaaagaact gaaagattta gatagccacg catgggaaag cgacaacgaa accagtacta
     3421 atgtggtgga cgctttagaa tctgtcgatg aattagaaag taatttagac caaaccaaag
     3481 aatctatgta caaactaagc tatgtggtac gggtatcagc aaagaatttg gacgagctga
     3541 aacaacgttg tgatgaagta aaagattttt atgatgatct gtctatcaaa ttagtacgac
     3601 cttttggcga tatgctaggc ttgcataatg aatttattcc ctcaagcaag cgatatatca
     3661 atgactatat tcaatatgtc acgtcggatt ttttggctgg tcttggtttc ggtgctacac
     3721 aaatgattgg tgaaaattct ggtatttacg tcggttacaa tctcgataca ggcagaaata
     3781 tttatctcca acctagcctt gcaagtcaag gaattaaagg ctctgtaaca aatgcattag
     3841 cgtccgcttt tattggttca ttaggtggcg gaaaatcatt ctctaacaat atgctggtct
     3901 attattcagt actctttggc ggacaggcaa ccattgttga cccaaaagct gaacgtggtg
     3961 aatggaaaga aaccttacct gatattgccc atgaaatcaa tattgttaat ctcacgagtg
     4021 agaacgacaa taaagggtta cttgaccctt atgtgatttt aaaactcccg aaagattcag
     4081 aaagccttgc cattgatatt ctcacgttct taacaggtat ttccagccga gatagcgaga
     4141 agtttcctgt cctgcgtaaa gctattcgga gtgtaactca aagcaaacaa cgagggttgt
     4201 tattggtgat tgatgaattg cgaaaagaag atacgcccat cagcaatagt attgctgacc
     4261 atatcgaaag ctttgtcgat tatgattttg cccatctgtt attcagtgat ggcagtgtca
     4321 cccaatccat tagcttggaa aaacaactca atattattca agtggcagat ttggtcttac
     4381 ccgattccaa tagctctttt gaagaataca ctaccatgga attattaagt gtggcaatgc
     4441 tgattgtcat tagtaccttt gctttagact ttatccactc tgaccgttcc atttttaaaa
     4501 ttgttgacct tgatgaggct tggagttttt tacaagtggc acaaggaaaa gctctatcta
     4561 tgcgattggt tcgagcagga cgtgccatga atgcaggggt ctatttcgtt actcaaaacg
     4621 cggacgattt actagatgaa aagatgaaga ataatattgg cttgaaattt gcctttcgtt
     4681 ccactgacct agtggaaatc aaaaagacct tagaattttt tggtgtggac aaggaagatg
     4741 aaaacaatca gaaacgctta cgtgaattag aaaatggaca atgtttagtt tcagacatct
     4801 atggtcgtgt aggggttatg cagtttcacc cgatttttga agagttgctt catgcctttg
     4861 ataccagacc gcctgtgcga aatgaggtgg aagaatgaca caaaaacaaa agaaatttct
     4921 tcactatagt tggataacgc tcctttgtgt gggcgttatt ttacttttat taggcacgct
     4981 gggcaatgca gtacaagcga caggcttagt tgatgaaacc attgatacaa gcaatgaata
     5041 ctccaagtat ggtttgaatc aatatcagct tgattattat gtggacaata gctggggctg
     5101 gcttccatgg aattggagtg atggcatagg acaatcggtg atgtatggat tgtatgccat
     5161 tactaatttt atttggacaa tttctcttta tctatcgaac gccacaggtt atttggtgaa
     5221 agaagcctat gccttagatt tcattagtca aacatcccaa gcaattggcg aaaacatgca
     5281 gacgattgca ggcattacac cgaatggctt ttccagttca gggttttatg tcggtttcct
     5341 ccttcttttt attctcattg ttggaatcta tgttgcctat acaggattgg ttaagcgaga
     5401 aacgacgaaa gtgatacgag ctgttttaaa ctttgtcgtg gtgtttattc tatcggcttc
     5461 ctttattgcg tatgcacccg attatattgc gaaaatcaat gatttttcca aagatgtcag
     5521 taccgccagt ctgaatattg ggacaaaaat tgttcttcct aacacggata gtcaagggaa
     5581 agacagtgtg gacatgatac gtgacagctt attctctgtt caagtaaagc aaccatggct
     5641 attgcttcaa tatggctcca ctgatgtaga agcgttaggg gaagaacgtg tggaaagttt
     5701 attgtctacc agtccagata caaacaatgg tgaagataga gaaaatgtag tgattgaaga
     5761 aattgaagat catgacaata cctatttaac cctaccgaag acgatttcca gattaggaac
     5821 agtcttcttt ttgtttgtat tcaatattgg tatctcgatt tttgttttcc ttctaacagg
     5881 aatcatgatt ttttcgcaag tgctatttat tattttcgct atgtttttgc caatcagttt
     5941 cctattaagt atgttgccga gttttgatgg catgggcagg catgccatta ccaagttatt
     6001 taatgtgatt atgacaaggg ctggtattac cttgattatt accgttgcct ttagtatttc
     6061 cactatgctt tattcgctgt cagcaagttc tccctttttc atgattatgt ttttacaaat
     6121 tgtgaccttt gcagggattt attttaaact gggcgattta atgagcctat tcactttaca
     6181 aagcaacgac tctcaaaaca tgggcagaca agtgtttcgc agacctcgtc aaatgatgaa
     6241 tcgacaaacc agaagatttc aacgcagtat gagtaagatt tttcgtggaa aagcagggtc
     6301 aacgaagcaa aagacaatga caacaaagca gaatcaaacc aataaagcta accatactcg
     6361 tcaaaatgaa cggactacac caaagaaaga aacagcactt tccaatgtga aaaagcaaca
     6421 aggcggtatc gggcaacgat taggtgaaaa gacagcccat gtattggaca cgaaaaatcg
     6481 tctagtcaat aaaagaaagc aggcaaaaca acagatacag gcgacaccaa cccatgtcaa
     6541 atatgccctt catcaaggaa aagagaatgt cactcataat gtaacagact ttaaacaatc
     6601 tatggcagat accaaagctc aacgcaaaca acagcgtatg aaccagcagg agcaaaaacg
     6661 aaagaacatt gctcaaaagc gtttggagct ggataaagca aaagaaaaaa gacgaaaaga
     6721 aaacccatca tctcctgttc agaataacca gcagcatgtt cgcaagcgtc agcaaaccgt
     6781 cacgcctgtt ttgtcacaaa acactaaacg tgaaaatcat cagcgaccat tgaaaaaaga
     6841 gcaaaaacaa aagacaccac cgaagcaatt caatcagagg aagctaaata aaagaacgga
     6901 taagcgagga caacaaacat gaagctaaaa ataacagtga tttgtagtgt ggtcttctct
     6961 ctcttctttt ttctcttatt attcctagtc attttctttg cagatgatac ggacagtggc
     7021 gaaaacaaca aggattcttc tattccacaa ggcggtgtga ccgtttcacc agaagtgttg
     7081 gcacaccgtc cgttgattga aaaatatggt aaggaatatg ggattgaaga ctatgtttct
     7141 tatatcttag ccattatgca agtggaatcg ggcggtacag cagaagatgt tatgcaaagt
     7201 tccgaatctt tgggtttacc gcccaacagt ttgagtaccg aagagtccat caaacaaggg
     7261 gttaaatatt tcagtgaatt attaaccagt gcagaacaac aaggagtgga tatagattct
     7321 gttatccaat cttataatta tggcggtggc tttttaaatt atgtgaggag tcatgggaaa
     7381 aaatatacct atgaattagc cgaacagttt tctaaagaga agtcgggtgg tcaaaaagca
     7441 gactatccca atcctatagc catacctgtg aatggtggtt ggcgatataa ctatggaaat
     7501 cagttttatg ttcagttggt ttcacaatac ctaaccgaca cttccccaac agaatttgat
     7561 gatgaaaccg tgcaagtcat tatggacgaa gccttgaaat acgaagggtt tccctatgtc
     7621 tttggagggg cgtcgcctac cacctctttt gattgtagtg gcttaataca atgggtgtat
     7681 gacaaagctg gcatttctct ccctcgtgtc gcccaagatc agtatgacgc tacgcaagaa
     7741 atctctatgg aagaagccca agcaggcgac cttatcttct ttcattcaac ctataatgca
     7801 ggcacatatg tgactcatgt ggcgatatat ttagagggaa atcgctttta tcatgcaggc
     7861 gacccaatcg gttatggcga cttaagcagt cgttattggc aagaccatct gattggggct
     7921 agacgtgtta ttcataacta agaaaggaat gaatcaaatg attataaaaa tcgagcgaaa
     7981 agaaaagaag gagagaccaa agaaatctaa aaaaccgagc aagcaaagaa aagttccgat
     8041 gattaaagtt ggcacgcata agaaattaac ctttgtttta tgggttttat tgattggtag
     8101 tgtcggattt ggaatctata aaaattttac tgccattgac actcatactg tccgtgaaac
     8161 agaaatcatt aaacagcaga ttgtcgatac caaccaagta gaaagttttg tgaaatcctt
     8221 tgcgaaagac tacttttcgt ggcaacaatc ccaagaagcc attgataaac gaaatgaaaa
     8281 attgacccat tatttgacgg aagaattgca ggtattaaat gaagaaatga ttcgtaagga
     8341 tattcctact agttctagtg tgaacgatat acaggtgtgg caggtttctc aagtgaatga
     8401 gaacaccttt gaggtactgt tttctgttga acaagttatt accgaagaca aagacaagga
     8461 aactatatcg tctagctttc atgtggttgt ccatatagat gaatcagata atatggttat
     8521 tatcaaaaat ccgacaatga gtaagaaacc tcaaaaatcg gactaccaac ccaaacagct
     8581 agaaagtgac catacagtag atacagaaac gatggacgaa atcactagct ttttagaaac
     8641 gttcttccag ctctatccta ccgccacaga aaaagaattg acttactatg taagtaatca
     8701 tgtgttacct atgattaaca aggaatatgt tttcgaggaa ttggtgaatc ctatctttac
     8761 cagaaaagat aaccaagtaa tcgtgaatgt cgctgtgaaa tatttagatc aagaaacaaa
     8821 ggcaacccaa atctcgcaat ttgagttgat attagaaaaa caagataatt ggaagattgt
     8881 aaaataacaa atattggtac atgattacag atactttgta atcatgtact ctttttgata
     8941 aaaaattgga gattccttta caaatatgct cttacgtgct attatttaag tgactat
//
LOCUS       DAZKCY010000100         8871 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000100 DAZKCY010000000
VERSION     DAZKCY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.101"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..299
                     /locus_tag="ACG8XC_002170"
     CDS             <1..299
                     /locus_tag="ACG8XC_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191845.1"
                     /translation="DYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPV
                     RTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(396..830)
                     /locus_tag="ACG8XC_002171"
     CDS             complement(396..830)
                     /locus_tag="ACG8XC_002171"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9191846.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1062..1772)
                     /locus_tag="ACG8XC_002172"
     CDS             complement(1062..1772)
                     /locus_tag="ACG8XC_002172"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9191847.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1762..2622)
                     /locus_tag="ACG8XC_002173"
     CDS             complement(1762..2622)
                     /locus_tag="ACG8XC_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP9191848.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2627..3265)
                     /locus_tag="ACG8XC_002174"
     CDS             complement(2627..3265)
                     /locus_tag="ACG8XC_002174"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9191849.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3280..3579)
                     /locus_tag="ACG8XC_002175"
     CDS             complement(3280..3579)
                     /locus_tag="ACG8XC_002175"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9191850.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3622..5106)
                     /locus_tag="ACG8XC_002176"
     CDS             complement(3622..5106)
                     /locus_tag="ACG8XC_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9191851.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5126..5587)
                     /locus_tag="ACG8XC_002177"
     CDS             complement(5126..5587)
                     /locus_tag="ACG8XC_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9191852.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5605..6672)
                     /gene="ulaG"
                     /locus_tag="ACG8XC_002178"
     CDS             complement(5605..6672)
                     /gene="ulaG"
                     /locus_tag="ACG8XC_002178"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP9191853.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6890..7660
                     /locus_tag="ACG8XC_002179"
     CDS             6890..7660
                     /locus_tag="ACG8XC_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9191854.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7933..8760)
                     /locus_tag="ACG8XC_002180"
     CDS             complement(7933..8760)
                     /locus_tag="ACG8XC_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9191855.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2701 a         1864 c         1385 g         2921 t
ORIGIN      
        1 tggattatac tgttgaattt gacagtgaac tacttaagca atttccacaa gaagaacatt
       61 tcatgtataa agaagcaatt tcccacaaag tgacagagta tttggagcat aatttttttc
      121 gtataaagcc agtgcgtact cgttcggcaa aaggaatata cgagatgaaa gtcaaaatta
      181 ataaaaaaaa ttatcgcata gcatttgctt taaaaaaaaa tcatgtgttc attttttata
      241 ttagttcgaa tttgcaaaaa attgtatttg ataaagaggt gagtaaaaaa ataaaatagc
      301 tgagcattgg aggatagatt gagtgatact aagtctatcc tcttgttttt ctaaagaact
      361 aagctagaat tttttctttt gtacgttatt atatgttatt tgtcattacc aaaaagcacc
      421 ttttttcttc tagtatctat gacataagaa ataccaccca tttcctttac ttgaacaaat
      481 cctactgatt cataaaattt ttttgttttc tcagtatcat ctgtcaataa ttcgatttgg
      541 actggtccat aactagtgat cttctccaaa gcttcattca aaagcacttt gcctattcct
      601 tggcgctgat acctcggatg aatgaggata tcttgaataa atgcgatcgt cgcaaaatca
      661 gtgatccaac gaacgattcc gataatcttc ccttccccat tacgctttat cagagattca
      721 gattgttcaa atgctctagc taaccttgca gtgtcttttg tgtaattcgt ccaaccaacg
      781 ctcttatata attcagaaat ctcatcgatc ataagggaat caatattcat ttttttcttc
      841 ctttcttata caattctccc tctaataata cagttctttg actatttaat ctatcacata
      901 taactctcct gtaaagattc tctcgattcc ttaaaattcc tctttccttt tagaatgcat
      961 actttttgat aaataaaaag gttgtgataa attttcttca caacctctgt ttctctctat
     1021 caagtgttat cttttaattt tctaccctta ccctagcatc tctattgccc gtagtatgca
     1081 tactttccat gtttgcgaaa ataatgttta tctagtaaat attgcgggat ttcacatata
     1141 ttggggttga tcagctcgtt ttctttggcc atcagacaaa tttcatctaa gatcaaactg
     1201 ttttctactg ctttcatagg tgaatccccc catgtgaacg gtccatgtcc atagacaagt
     1261 actccaggga cttcgtttgg atctaatttt ctttccttaa acgtctcgac aatcacattc
     1321 cctgtatgaa cttcgtaagc ttctcttact tcttctttcg ttaattggcg tgtacaagga
     1381 accttaccat aaaaagcatc agcatgagtc gtgccataag ctggcaaatc gcgtcctgct
     1441 tgcgcccaca tgacggagtg cgtggaatga gtatgagtaa tagcagttat atcttcaaaa
     1501 gtttgataca aaacaacatg tgtggggaga tctgaagatg gttttaagct atcttcttct
     1561 attatatttc ccgacaaatc tgttaccacc atttgatctg cttgcatgca ttcatatctg
     1621 acaccgcttg gcttaatgac gatgactcct agctcacggt ccacttcact cacattgccc
     1681 caagtcaatt tgactaatcc gtatcgcggc aatgccagat ttgcttcaag gacacgttgc
     1741 ttcattgctg caattttttc ttcacaagac atcatagcca gcctccttca atttagaact
     1801 taagtagtct tttgcctgct ggatttctct tcgaaagtct tggctttttt cactccacat
     1861 ttcaatcaga aatgttccat cataatctag acgtttcaaa tgtcttaata atccaagaaa
     1921 atcaacacaa ccactaccaa aaggaacatc acgaaactgg cctgaatatt cttttgttac
     1981 agggtaagta tcttttaagt ggatagcagt gatataatcg atccctttct caagttctcg
     2041 agccggatta ttttccggcc atgcggacaa attgcctagg tctggatata cttgtagaaa
     2101 aggtgaatga atctgttctt tgatttctaa aaattttgaa atggagttca taaatggatc
     2161 atccatgatt tccacggaaa gaatgatgcc atatttagaa gcttccttta cgctttcttt
     2221 tattccttcg ataaagtctt ctctggaatt cattgatttc tcttcataat aaacatcgta
     2281 tccagctatt tggattactt tgacgcttag attatgcgcc aaatggattg cttttttcat
     2341 tagttgtttc gctgcttttt tcttttctgg atcagcagaa ccaaacggaa atcgccgatg
     2401 cgcacttaaa caaatcgaat ggatacgtac accggagctg aacatctctt ctctcagctc
     2461 agctatcttt tcttcagacc aatctaatcg tgctaaccgc tcatccgtct catcaatcga
     2521 catctcgata aaatcgaagt ccatttcttt tacaagggaa aaacgttctt tccatgaaat
     2581 atctttgggt aacgcttttt cataaatccc aatttgagcc atttccttac ccccagatac
     2641 gaatgatctc atctttgaag ttttgtgcgg ccttcatcgg atcatttgct gctgtaatac
     2701 cacgaccagt gataaaggta tacacatcga tcccttcaaa taagtgtaat gtgtgtggat
     2761 ttaatccacc agttacagaa acattaaagc ctagttcaat cagttttttg atcttcgata
     2821 aatctttttc accccaagtt tcaccagata acagcgcatc acggctttga tggtagatta
     2881 cctgccggat accgatgtta tgccaatcca tggcttgttc aaaagtccaa ttgccgtaga
     2941 gttctacttg caattcacct atttcttttt gtgctgcctc catagttgga atcgttgcac
     3001 aacaaatgac tgtcatccag tctgctcctg cttttgcaac attactcgcc actgttcctc
     3061 cagcgtctgc acatttcgta tcagcgacta gttttttatc tgggaacata ctgcgaatac
     3121 agcgaatcgc tttttgtcct tcctgtaagc aaagaattgt gcccacttca ataatatcta
     3181 ctacatcacc aactttcata acatctccta atgcatgttc taagtcattg tgatctaacg
     3241 caatttgcaa attcggcctg ctcatcttag ttccttcctt tacttgtttt tttggtacca
     3301 ctttgtttca gcaaattttt cactgatttc tttgactgac atcacatttt tgataccgat
     3361 aactgtaact ccttttttct ctgcctgagt aaacatattc aaaaaattga ctggcgtaaa
     3421 aactacatcg tattggccag ctgaactttt tccctctgaa atggcacaat gatggatttt
     3481 ggtaattggt acgtttagtt ctttcattgc tttttccaca cttctcatca tcatcaagct
     3541 tgttcctgat ccatttgcac acgatactaa aattctcatc ttaatccctc cattatgagt
     3601 gttgtctaat cagattttct tttatactct ctgcttttct gcatatgtgt catagtcttc
     3661 tacaatcaaa aaataaccat caggattttt ccgatattgt aactgcggaa tagccaataa
     3721 accaatgaca actacggcaa ttccagcata acctaggact ttcatgatta ctgtaaacaa
     3781 tggccaagct gttgcccagt cgaacatccc gatataacca ccgtatgttg acatcccaat
     3841 ccatgtagcg aataatgctg aaccgcctac ttgtatgatt cctgaaagaa acgggaacag
     3901 gcaagcggct ttgaatccac ctcggttatt ggcataaact gctataacgg cattatcaaa
     3961 gaacaacgga atgaaaccgg ctatgacgat cgttggtgat ttcaataaaa tcagtaaacc
     4021 aatcatcaaa aactgtccca aagcaccaaa caagaaccca atcgttaccg catttggcga
     4081 accaaagcca aaagtagcag ctacgtcaat tcctggaaca gctcctggta aaatcgtgtt
     4141 tgagattcct tggaaagatt gagttagttc atcgacgaat gtccgaacac ctaattgcaa
     4201 gatcgccaga tataccgcaa aatttaaaga agtcgtcata atgtagaata agaaactctg
     4261 cccttcttgc atgaattgcg cttgaatcaa ataatctttg cctagtacaa tcagaatgat
     4321 cccaaagaaa aatagcatga gtaacgaagt agcaaccata ttctcattga aaatcgacaa
     4381 gaatcctggt aatttgatat cttcaatttt tttgtcttgc ttgattgatc tatttttctt
     4441 ttcatcccgc tttttcatcc actcagctaa ttttgcaaat ataaagattc cgaacatttg
     4501 ctgatgtgcc actgcaaatc cggcaccttc tgtcaattcc tgcgtgattc ctactgttaa
     4561 gttagaagct actgcccaat aacaacctaa aatcagtccc ataaaaatca atacttgtat
     4621 cctacctaga tcaggaaagc aaaacagtaa aatccaaaaa gcggtagctg cttgttgaac
     4681 ctgaacgttt ccggtagtga aaacggaacg cagtttcgta tatttctgaa aacggactaa
     4741 taagatattt aaaacaaatg caatcagtaa taaaatcatc acatctccaa aggtacggcc
     4801 aaatgtctcc attaatccag catccacagc attttgtcca aaataaggat ctgtaaccat
     4861 ggcatccaaa ttgaaccgtt cctttagtcc aaccaagatt ggccgaaaat tattgaccaa
     4921 accaccagag ccaacagtca aaataaagta accgaccgtt gcctttaaga atccagcgat
     4981 gcattcatga agcgggcgtt tcagcaaaat atagcctaat aacacgataa aaccaatcag
     5041 gtatgctggc tgcgtcaaga tatttgttgc aaaatacgtc caaatgctta acaaaaattc
     5101 ccccatcttt tttctcctct atcaattaat tatattctgc catcacaact tggtaatcat
     5161 cgatggagtt cgtagagatg agacgctcaa ccaatccatc tgtcattaat aattcggata
     5221 atttttggat attttctaga tgttcttctg gatcttttgc tgcgagtgta aaaaacaaac
     5281 tagccgtttt ctcctcattc tccccttcac tttcaaaacg gattggctgt ttcaacttgg
     5341 taaatgaaat agctgttcca aaaacaccat cacttttttc tgaagaatgc ggcattgcta
     5401 cttgaggtgc gatcacaata taaggaccgt atttttctac acaatggatg atctcctcca
     5461 catacgtttc atcaataatt tccttatcga tcaatacaag acagctttcg gcgatagctt
     5521 cacgccaatc cgtgatttct ttatctgggt aatgaatcaa ttgatggtca aaaaagtatt
     5581 tcagcaagaa actccccttc tttcttataa aaacgacgga aacggtgtat cattttcaat
     5641 cgagaagaca tcttcaaatc ctcgataaaa attaaactca agatcatctt tgtttgtcgg
     5701 atagatgaat ttcccgccta cttgccagat ataaggttta aatccatact tcaggcgatc
     5761 ctttttgaat ttccaaattt cagtgatctc ttttggatca gccataaaat tagaccaaat
     5821 atcgtagtgg acaggaatca ctacttttgc attcaatgac tccgccatgc gcagcatatc
     5881 aacagaagta actttgtctg ttatccctcg tgggttttca ccataggccc ctaaacagac
     5941 atcgattttg tgttcattac catgtttggc aaataaattg gagtaatgtg aatctcctgc
     6001 atgataaaga ttccctcctg atgtttcgaa taaatagttt actgcaattt gatccatctc
     6061 ctgaggcatt tttcctttta atacctcttc tggatcttca caagtgacca acgctgtccg
     6121 atcaaacgct tctaaagcta ctacattgat gtctttgatt ttgactgcat cacctggctt
     6181 tactgtaatc gttttttctt caggaattcc ccatttttgc cacgtgcgaa ccacttcttt
     6241 tggcccaatg aatgttgctt caggacaatt ttgatgaact gctgcggctg tatggatatc
     6301 tagatgatcg gaatggatat gtgtaacgat taaagcgtct acatttttta tcgcaaaagg
     6361 atcgattacg aaaggctgag tacgtaaatt aggctgcata ttttgaacgc cactcatacg
     6421 catcatctga tggccttttt tcatcatgcc attcccatga ctacgttttc ccgtcccaca
     6481 ccatagatca cataaaatat tcgttccttc atgacttttc agccaaattc ccgtacagcc
     6541 cagccaccac atagcaaaag ttccaggatc cactacttct gtttcgattt cttcattcaa
     6601 gtatgtgccc cactcaggaa atgtttctac taaccacttt tcttttgtta cttcattgac
     6661 agttttactc atttgcttgc tccccccata tcgttgttcc aacttacaag taaatattaa
     6721 tgtgtatcag catccatgaa aaccctttta tatgaccgtt tgttttttat atgattttct
     6781 ttcagtaatc aatgattgtt tcagtataat aaagacagat atgaatgttt aaatgaacaa
     6841 ctagtcaaat aaatccatgg atcaagatgt ccctcaggag gcaaaagtca tgaaaaactc
     6901 acttgtcaat attgaaaaac gtcaaaaaga aattttggaa ctgcttcagc agactgaaca
     6961 actatcaact ttcgaattag ctgaatccct tgatgtatct attagtacga tacgaagaga
     7021 tttaaattta ttagaagaaa aaaacgagat catacgcaat tacggttatt gttcttataa
     7081 ttacaaaaac cagactgatt ttgatcaatc tggtcctgaa aggattaaac aagcgattgc
     7141 cagagaagct agtaaataca ttaacgatta tgatacgctg tttatcaatt ctagctcgac
     7201 tgcgttgaaa acactggatt ttcttcaatc taaccatttg acagtcatta caaacaacgt
     7261 gaaaatcaat tactctccgc acaaagataa ttgtagttat attttaactg gtggagagat
     7321 gcgctttcca aaagaagcac tagtgggcga catcgcatta aatacgattt cttcagtcaa
     7381 tgcagacatt tgtatcattg gctgtagcgg agtcgatgta gtacatgggg tcacaacaaa
     7441 gatattaaat gaatcaacga tcaacgaaat gatggtcaac aagacaatca aatgtaagat
     7501 tttagtcgct gatcatcgaa aaatcggact gacatcaaag ttcaagattg ctgatatttc
     7561 agtctttgat tatttgatca ctgataaatt ttgttctgaa aaagtattag aggagatcag
     7621 aaatacgggg ataaaaacca ttcaagtaga ttcattttaa gaaccaggac gtaaatatgt
     7681 aaataggatt atcaaaaaaa tgaataacaa atttatcgat aaatgaaaaa cacgctacgc
     7741 tcaaattgat aaaggaatcc caagatgtat ccatccaatc gataccatca taatctattg
     7801 cttcttttat aaccgttata tgttgcctaa ataggaatca aacaatatcg aaaaaaggag
     7861 gtatgacaaa agtcacacct ccttttttcg aataaacggt attcaaatca gaacactgat
     7921 ttcacaacca ctttatttca ggtcgttttc aatcaccttt accgcgtaat catttccgtt
     7981 gaaacgcgct attttttttg cgtccatata tctttctaaa taatcatctt ctcctaattg
     8041 cttatggcaa gaagcaattt cataaagcaa gtattccaat gcataagtcg atttatgttt
     8101 cttattatac tcaataccct tctcacataa ttggattgct ttatcataat cctcgatttt
     8161 acggtaatag gtggcgatat cataatatac aatgcagacc atcagattat caaaatcagt
     8221 ctcactcaat attttaagtg tacgctccac ataaactttc gcaaaatcta attctccttt
     8281 tgacaagtag atcattgcca cgtttgcagc tgcttgtaag tgatggatat ctgtttcggt
     8341 cgtttgttcc aacacttgat taaaataaaa taatgctgca tctggttttt tcgcataaag
     8401 atgatcaagc agtccttgat aaaataaaat ttgatttaaa agatcagaat tacttacttt
     8461 ttcctgatcg atcgtttgta aaattttata agattcttgg tattttgata cagaattcaa
     8521 tgattgaatc tcatttattt ttttcctgat tttgtcttct tctgtttcta ctaatatatc
     8581 ggagatctcg acattcagct ttttgcaaag cgcacttaag atattgatgc ctggcgacat
     8641 attcttgttt tcgatattgc tgacagttgc ttgttcacaa atgccttctg ctaattgtac
     8701 ttgtgttaac ccctttttct ttcggtattc ttttagtaat gctccatcta ctttaatcaa
     8761 aacaatttcc ttccctttaa aaaattattt ctcctttata tcatacacaa aaaaaagttc
     8821 taaacaacca aaaatgttta gaacagaata aaaattttaa ttttaaaata a
//
LOCUS       DAZKCY010000101         8730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000101 DAZKCY010000000
VERSION     DAZKCY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.102"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..605)
                     /locus_tag="ACG8XC_002181"
     CDS             complement(60..605)
                     /locus_tag="ACG8XC_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP9191856.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(617..1309)
                     /locus_tag="ACG8XC_002182"
     CDS             complement(617..1309)
                     /locus_tag="ACG8XC_002182"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP9191857.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1328..1612)
                     /gene="macP"
                     /locus_tag="ACG8XC_002183"
     CDS             complement(1328..1612)
                     /gene="macP"
                     /locus_tag="ACG8XC_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFP9191858.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1625..2188)
                     /locus_tag="ACG8XC_002184"
     CDS             complement(1625..2188)
                     /locus_tag="ACG8XC_002184"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP9191859.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2416..3078
                     /locus_tag="ACG8XC_002185"
     CDS             2416..3078
                     /locus_tag="ACG8XC_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFP9191860.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3202..4398
                     /locus_tag="ACG8XC_002186"
     CDS             3202..4398
                     /locus_tag="ACG8XC_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFP9191861.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4499..5875)
                     /locus_tag="ACG8XC_002187"
     CDS             complement(4499..5875)
                     /locus_tag="ACG8XC_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9191862.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5912..6778)
                     /locus_tag="ACG8XC_002188"
     CDS             complement(5912..6778)
                     /locus_tag="ACG8XC_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP9191863.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6867..7892)
                     /locus_tag="ACG8XC_002189"
     CDS             complement(6867..7892)
                     /locus_tag="ACG8XC_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9191864.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7910..8584)
                     /locus_tag="ACG8XC_002190"
     CDS             complement(7910..8584)
                     /locus_tag="ACG8XC_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9191865.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
BASE COUNT         2689 a         1915 c         1520 g         2606 t
ORIGIN      
        1 gttttatgct cttcttttat tattttgtgc tttttatttt atcaaatcac tttgtttttt
       61 tataaaattt ctgcgcctag tgtggactca aaatgtctca atgcccattc atgtccaacg
      121 ggatcgaaac ttgccacggc atctttgaaa atatggagtt tgtatcctag attatacgca
      181 tcgactgctg tatgcaacac acagatatct gtacatacgc cggtcaaata aatatctgtg
      241 atctggcgtt ctcgcaaacg gatgtctaga tctgttccac taaaagcgga ataatgccgt
      301 ttgtctatcc aataaacatt ttcctcttgc tcatagtctt ggtataaact ttttaaagag
      361 ccgtacaatt cccgtccacc cgttcctatc acattatgcg gaggaaataa gcggttctcc
      421 ggatgaaagg aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa atccttttga
      481 tcaataaact tctttgtatg agcagtcagc tttttttcaa ttacttgtcc tgctgtccca
      541 gtagtcagct ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg
      601 ttcatcttag tcctccttat acgagatgtt tgacaaattc gatcaccatt tctgctgatc
      661 gtttgcctgc gtcttcgata aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt
      721 cgctcattgc acgtacgatc aaaaatggga tattgaactg acgagctgtt tgtccaacag
      781 cggctccttc catttcgcaa gctaatgctt cagggaaatt cgttaagatt tctttgattt
      841 tatctggcga gtcgacaaat gtatcacctg taacgatcaa tccttctttt gcgttcaaat
      901 tcgttgcttt tgctgcttta accatttcac tgcgcaaata tgtgcttgct tcataatata
      961 gaggcattcc tggtaattgt cctgggttgt agccaaatcc tgttgcatct gcatcaaaat
     1021 aagctacttt gtctgatata acgatgtcac ctacttgtaa gccttcaccg attccgccag
     1081 ctgaacctgt attgatcacc atgttcacgc cgtattgttg gatcaacaga cttgtggtaa
     1141 tagatgctaa gacttttcct ataccggaac gtacgacgat cacttcgtgg ttacctaatg
     1201 aacctgaaat aaataaagcg cctgcgcgtt cccatgataa tggttcgctt aggttctctc
     1261 tcaaaatttt tacttcttct tccattgcgc cgataatacc gattttcatc tttttaattc
     1321 cttctttcta tataaatgcg attgctaaaa aaaccacaca taacattagg ataacaataa
     1381 taagagattt catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg ctgatccttg
     1441 ttttcgtgat tggtttattc tttttgcttt ctttacgata aaagctggca atcttttttt
     1501 cttcttgctg ataagctgtt tctgcttttc gctgtttttt taatgactcg cttgcttgtg
     1561 cttgttgtcg cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc ataagccgat
     1621 ccctctactt tcctttggtc aatagttccc agtattgaat cgcataaatc gtctttgcat
     1681 cacaaatcaa ctgatctttc atcgcttgtt gcgcctcttc aagagttaga tgatacaact
     1741 ccagtacttc gtcttcatct tgtgctaaag gattctcgac cttctctacc ccttgtgcat
     1801 gataaatatg aaggacttca tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc
     1861 tcagcgactt cgcacgatag ccagtttctt cttctaattc tctagctgct gtcatttcgg
     1921 gattttgtcc ttctcctgga tcgatttttc cagctggaat ctccaaaatc actttttcta
     1981 aaggttttcg aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc
     2041 cgacaccgcc aggatgaaat accagttctc tcttcgaagt tcctccatct ggcaaacgaa
     2101 cttcgtctac agcaacatca ataatttttc cgctatagat ttctttgcga ctgattgttt
     2161 tttcttcaaa ttgctcattg ttcagcatat cttctcctgc tttctttgta atcataactg
     2221 cttttttttc atttttcaat ttttctcaaa aactccttat tagtttacca tagataaacc
     2281 ttagattttc tctaaatata gaaaatcctt ctttagtccg atactcttta tagtagtccg
     2341 tatgctaaaa taagtatgta atcgaataac gaaaagaatt caatactcaa cgataaatga
     2401 catggagggt ttattatgat aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta
     2461 tttctctttg tccgaaacaa tagtttttct tttgttctag gacttttatt gataggtgta
     2521 gttttagttt tgtggggact ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt
     2581 cctgcactga ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa
     2641 atttcatttt ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca attgcaaatg
     2701 aatatgcagc agacagccaa acttaaagca atcgatctcc gccatgatcc agtcaaagca
     2761 gcgaaagctt tattcaaaga attggtgaaa gaaccaaacc gactgcatga agcaagtcag
     2821 tttctttata cgcatctgcc gaatctagtt gatttgacaa ataagtataa tgagatcaat
     2881 gaccatgaga tcaaaaacaa acagacctat gaaaaactag aagaaagcgc gcagatcatc
     2941 gaccagctgt cagctttaat cgcacaagat tatcaaaaat tcgttgctga ggatctagat
     3001 gatatcgacg tagagatatc tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa
     3061 ccagaacaaa aggattaact cttttgttca taaaagaaga ctcggaacat tcagatgatg
     3121 tctggctgtt tcgagtactt tttctaaagt agagacattc atgtaagaat cgagaacaga
     3181 ttcaacaagg aggaattttt tatggatcat aaaccgactg aaacaaaaga tgttacacca
     3241 gttaacgata cacttgaaga tttattgaac aacccgtttt caactccaac agatacgctg
     3301 accacttcac aacaagcgga gattgatcag ctccaagagc aacagacagc tgctcgttta
     3361 attgacaaac taccggcgga acgccaagaa caagctaaac agctagcagc aaaaatcgat
     3421 gctaatgatg cacagtctgt catcagctac ggatcggctg cccaagcaaa attaagtgaa
     3481 ttctcacaat cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt aggagattcc
     3541 ttaactgaac tgatgtatcg tctccaagaa gccaatcctg acgaactgcg tgccggagaa
     3601 gggaacatct tccagcgtgt cttcgggaag gtcaaacaat cgatctatga agtgacagca
     3661 aaatatcaaa agattggtgc acaaattgac aagatttcag tgaaattaga taaagaaaaa
     3721 gacggtctgc taaaagataa tctgatgtta gaacagcttt accagaaaaa caaagactac
     3781 tttgatgcac tgaatattta tatcgctgct ggagaattga aaatggaaga acttcaaaca
     3841 acgatcattc cagaagcgat gaaaagagca gaagaaactg gcgatcagat ggatgttcag
     3901 attgccaatg attacacgca attcctagat cgtctggata aacgtacgca tgatcttcgc
     3961 ttagctagac agatcacgat ccagcaagca ccacaaatcc gtttgatcca aaacacaaat
     4021 caggccttgg ctgaaaagat ccaagcatct atcgctacag ccattccgct gtggaaaaac
     4081 caagtcgtga tcgcattgac cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa
     4141 gtctctgaaa caacaaacga cttattgaag aaaaattcag aaatgctgaa aatctcagcc
     4201 atcgaaacag cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct tcaaaccact
     4261 caaaatgatt tgatcgaaac aatccaagag acattgcgta tccaaaaaga aggaaaagaa
     4321 aaacgtcgtc atgcagaagt agagcttgga cacatggaag aagatctgaa acaaaaactg
     4381 ttggatttaa cccaatagat cacctttcgc ttgttactaa aacaaacgcc caacaacagt
     4441 ttattacaac tgttgatggg cgtttttctt atctttcttc taacttctcc attggagtct
     4501 atttctcatt catcttgctg tgtccatagc catagccaaa ataaatgatc agtccaataa
     4561 ccattacgat accgaacacg atccatgtca cagtttgcag acgaagcatc aatacaatac
     4621 aagtcaagat ggagacgatt ggcaatagcg gaacgaatgg cactttgaat tctccagctt
     4681 ttggtttgcc aaacattttt ctcaacttca gtaagcctag actaacaagc atcaaatagg
     4741 cgagcgccat gatattcgtt agttccgcta atacattcaa tgggaaaaat ccggcaaaga
     4801 atacagctgc cacaccagcg gcataagtcg cttttttcgg tgtacgatgc ttttgatcga
     4861 tttctgtcat aaacttagga agtaagccat ccttgctgac agcaaacagt aaacgagcaa
     4921 gtgaatacat catcgagatc gttaccgtca gtagcgttaa aattgcccct acagcgatca
     4981 ccgcgcccac tgttccatgt cctacatacc tcattgcaaa agccactggg tcattgactc
     5041 ctagttcagt atacggaacg attcctgtca agatcagcgt cacgatgatg taaaggacag
     5101 tagcaatcag tatcgaacca atgattcctc ttggcacatc tttttgcggg tttttgactt
     5161 cctcggctgc cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac
     5221 catctagcac accggaaaaa ccaaaaggca tgaatggctg ccagttatca ggtttaacga
     5281 aaaatattcc tacaatgata aacaacgcga tgataccaaa tttgacatat accatggcat
     5341 tattcaaacg taatgctttc ttggcttcaa gcgacacgat gtaagtgacg aatatcacta
     5401 cgagcaccgc aatcaagtca atgtatgtac cattttccgg attgtacccg ccagttaatg
     5461 cttgtggaag ttcggcccct aaacttttca aaaaaccttg catatagcca gaccatccgg
     5521 aagcaacaga ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct gtcagccagg
     5581 cagcaaattc accaaataca acatacagat aggcatatgg cccaccaatc actggcactc
     5641 gcgaagcaaa ttctgcgaaa cttagtccag acaaaatgac aacgattgca gctaagacaa
     5701 atgaaagtga cagtgctgga ccagcatttt gattggccgc aacccctgtc accacaaata
     5761 ttcccgtacc gatgatcgca ccaattccaa gcatgatcaa atcagacgtt tttagttctt
     5821 ttttcatctc actcgtatga ttgagattga tctctttctt acgaaacaaa tccattgaag
     5881 ttcctcccca ttttaggttg gaaaacagcg attaaactgt tttcatatac cctaaaagga
     5941 taccacctaa aatgactaaa aggcaaccaa caataacata aatgagctct tttttcgttt
     6001 ttctttcgcc aaggatgaaa atcccaccta aagtagagat aatgatcccc atttgtgaaa
     6061 gtgagaagcc aacagctaaa ccaagtgatt tgactgttag aagcatgcag atgtttccta
     6121 agccccataa taatccacag atcatgtttt tccacacaaa ctgatcgact ttaactttac
     6181 ggaaagcaaa gaagcttgcg cctaagatca ttccgatact ttgaggtaag ataattgaca
     6241 tcgcatccaa attcgcccag ttgacgatga tggtatatac accataacca atcgtagaga
     6301 agatcaatgc tctaaaaccc ttaccaaaat caagcatttt attatctgta tctatttttc
     6361 cttcgtcatc ttgtcgggca gtcaaatacg caccgcttac aagaagagct aaagcaataa
     6421 taccgtatac ataatctttc gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg
     6481 tattcaaaat taattgaaaa ccagtagaca atggtagccc tacagaaaca cccatgtact
     6541 tcattccatg gaactgtcca ttttgtccaa cactccaagc taagccggat aaaaatccga
     6601 tcaagaaaat ccaaggggtg attacaggac taacaacgaa aaagacaact aaagaaaaca
     6661 gaaatgcacc gatcgtcatt cctagtgttt gttgattggc atcgccacca attttaccac
     6721 ttactaaacc aatactaccc catgccaaca tagggactaa tgctattaaa atttccataa
     6781 ccaatctcct tttatttcac atgataagaa acttatcagc gaaaacgtta acaacacaag
     6841 cacttttttt cgtttctaac gattttctaa cgtttttcat tataaaaatt taagattggg
     6901 aaaaataaag tcattgttgt cccttttcct tcagttgatt cagccgtcaa atggatacct
     6961 aatttttcgg ccagattttt tgctaaataa agtcctaatc ctgttgaatg tgttttgcta
     7021 agtcgaccat ttctcccagt aaatccctta tcaaagatcc gtttcaagtc ttccgctggg
     7081 ataccgatcc ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt ttctatgctc
     7141 acaatgatgt ttccgtgatc cggcgtatat ttgatggcat tactaagcaa ttggttgaag
     7201 ataaaagcga tccatttagc atcggtcaaa acagtatgat cctcaccaac gatctcgtag
     7261 cgaatatttt tttggataaa ataatttgcc tgactgcgta tcacaggctg aatgattcct
     7321 tttagtccat attcttggat gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac
     7381 aaaacttgtt ctacgtattc atcgattttt gataattcat tttctagtaa tgtatacttt
     7441 tgatcgtcaa tatcgaactc gatcgaacga actagcaatt tagaagcgga aagtggcact
     7501 ttgatttcat ggacccatga gtcaatataa tctttttggt cttgttgatt actgattgct
     7561 gattgcatta cttcttggtg ttcacggatc agctgattga tatattcttc taccagtttt
     7621 tcttcttctg tcctcccacc actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg
     7681 gctaattttc cccaccatct tctcttgctc aagtaaccac ccagcagata aaaaatcaga
     7741 aagacgccct caatcaaagc taaataagca attgtgctca aatcaacttt gatgtttggc
     7801 gccagccaca tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc
     7861 cactgatctt tcaaatattt aaaaaaattc atcttaaccg ctcatccttt cagggaacga
     7921 tatatccctg tcctactttt gtttcgatat atccttggat acctgcctgt tcgatttttc
     7981 ggcgtaaccg attgatgttg actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc
     8041 acagtgctcg cagcaatttt tcccgactta agatctttcc attgtgcttc atcaaaataa
     8101 ataatagacg atattcattt ttgctcaaat caatgatctc tccgttgatt tccatacttc
     8161 cgttgtcaac attcaacgta atgccattgt ggctcatcat ttcactagat aattctgtgt
     8221 agttgtaaga acggcgaagt aatgcattga tttttgcaat cagtacatcg atttggaaat
     8281 gttttgtcac aaaatcatct gcccccatgt tcatagccat gatcatatcc atattcgtat
     8341 tcctgctgga gataaaaatg atcggtactt tcgatacttc acgtattttt tgacaccaat
     8401 aatagccatc caaaacggga agattaatat ctagtaatac aagctgtggt tcttctcttt
     8461 caaagtctga gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct
     8521 caagttcctc catgatcaat tgtcgaatga tctcttcgtc ttcgactacc atgatttttg
     8581 ccataaaaaa tccctccatc tttcttcttg ctactacaat atcaaagaag cccttgtgaa
     8641 acaagttggc ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg
     8701 cgatttaatc gcgtttgtct cttccaagca
//
LOCUS       DAZKCY010000102         8702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000102 DAZKCY010000000
VERSION     DAZKCY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.103"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            276..458
                     /locus_tag="ACG8XC_002191"
     CDS             276..458
                     /locus_tag="ACG8XC_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191866.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            442..627
                     /gene="tnpB"
                     /locus_tag="ACG8XC_002192"
     CDS             442..627
                     /gene="tnpB"
                     /locus_tag="ACG8XC_002192"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9191867.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            870..2390
                     /locus_tag="ACG8XC_002193"
     CDS             870..2390
                     /locus_tag="ACG8XC_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP9191868.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(2577..3149)
                     /gene="hxlB"
                     /locus_tag="ACG8XC_002194"
     CDS             complement(2577..3149)
                     /gene="hxlB"
                     /locus_tag="ACG8XC_002194"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFP9191869.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(3156..3818)
                     /gene="rpe"
                     /locus_tag="ACG8XC_002195"
     CDS             complement(3156..3818)
                     /gene="rpe"
                     /locus_tag="ACG8XC_002195"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9191870.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(3834..4349)
                     /locus_tag="ACG8XC_002196"
     CDS             complement(3834..4349)
                     /locus_tag="ACG8XC_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HFP9191871.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(4351..4635)
                     /locus_tag="ACG8XC_002197"
     CDS             complement(4351..4635)
                     /locus_tag="ACG8XC_002197"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9191872.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(4669..5997)
                     /locus_tag="ACG8XC_002198"
     CDS             complement(4669..5997)
                     /locus_tag="ACG8XC_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9191873.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(6011..6466)
                     /locus_tag="ACG8XC_002199"
     CDS             complement(6011..6466)
                     /locus_tag="ACG8XC_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9191874.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(6469..8415)
                     /locus_tag="ACG8XC_002200"
     CDS             complement(6469..8415)
                     /locus_tag="ACG8XC_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9191875.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         2899 a         1617 c         1368 g         2818 t
ORIGIN      
        1 aaagattcga ttaaattagt tgagtaaatg cttcgtacga acatagttga tttattcacc
       61 atacttccca ttacatcgta aaacgctata tttaagattt caaacaacta tattaatagg
      121 aagaaaactt catttttcct cttagttcta taaaaagtgt ggctaatttg aggcaaaata
      181 ttaaattaaa tgtaagcgtg tcataggata gtatctcgta cgccgattat ctttcttcta
      241 taattaaact atcaaatata gaaggagaaa aacgaatgaa taaaaaagat attgtaccag
      301 ttcaattttc aagtgcggct caaaaatcgc aaattgacac caagccaaaa ctagcttgca
      361 gaatcaaatc ggaagataag gaaatattta tttataatgg aatggataaa tatcttttgt
      421 ataccttgtt aaaagagttg aatgccaatg ctcattgatg caactgcatt gaaacatatt
      481 tttatcgttt gtggaaaaac agatcttcga aaaggaattg atggactagc agctttcgtt
      541 atagaagaat atgaccttga tgtttacgat caagcgcttt tcttattttg cggtacgaag
      601 aaagatcgct tcaaggctct ttactaggat caaaatggat ttgtattaat gtacaaaaga
      661 tttgaaagtg ggtaccttca atggccgcgt tccagaaaag aagtcttact gactggcctt
      721 tcaatcgaag aaaaatgtac gctaaaagaa acgaaaaaag gtgcgatata ttagacgaaa
      781 accttgttaa atcaaggatt ttgtctgttt ttcgttgtca taaaatggta taatagatgt
      841 aagaaaatcg ctagaatggt ggtgtaaaaa tgacaaaaat ggaatctgaa cttcttgtac
      901 aaatcaagca attaacacag ttgcttgaaa tccagcaaca ggaaaatact cttcttagag
      961 aacaaattgc tgaaatgaat cgacgcttgt ttggacgaaa aaaagaaaca ccacccgtag
     1021 atgggcaaat cgatttatta gacgactcaa cttttaatga accagagcac actggacaag
     1081 aaagccagga acccattacg gtatcttctt ttaaacgcag aaaacgcaag ggactcaaag
     1141 cactttcatt agaaggtctg cctgaagttc ctattcatta tgaattaaaa ggtgccgatt
     1201 gtttgtgtga agactgtggt catactttac atgatatcgg tgcgacaagg cttagagaag
     1261 agccattgta tgttcctgcc catatagaaa aacaagtcat ctaccaacat gcctatcatt
     1321 gcagagaatg tgaagcatta ggagaaacaa aaatcaaaaa agcgcctgtt cctaaaccgt
     1381 tgatcaataa tagtctaggt tcctcttcaa ttgtgacaca gagtatttgg gaaaaatttg
     1441 ttaaaaaggt gccggcatat cgacaagaaa aagaatggca tgcacttggt ttccctcttg
     1501 atcgtaaaaa aatcactaat tggcatatca aggtaagtga aatggtttta gagccaatct
     1561 acaattgcct tcatcaagaa ttaagcaatc aggaggtctt acacgcggat gagacaagct
     1621 atcgggtatt gaatactcat aaagaaaaga ccttttactg gttatatgct tcaggtaaaa
     1681 gcgaagaaaa acaagttgtg ctctacgagc acgcaaattc gcgttccgtt gatgagccaa
     1741 aaagtttttt agaatcattt agcggtttct tgcatactga tggttacgcg gcctatggaa
     1801 agctgccggt aaacaatgtt tattgttggg cgcatgttcg cagaaagttt tttgaagcat
     1861 tgggtaaaca accgtcccca aaaagtcatg ccaaaatagg attagacttc tgtgacaaga
     1921 tgtttcgaat cgaacggacc tttaataagc ttagcgcaga agaaaggttt gtagcgagga
     1981 aggaaacgtt gcagcctgtg atgactgaat tttttcattg gtgtgagagt tttgcttatt
     2041 tgccaaaatc gcagcttgga aaggcggttc aatacgcagt caatcataaa gacggacttc
     2101 agattgtgtt gcttgatgga cgcttggaac tttcaaataa ccgagcagag cgtgccatca
     2161 aagagttagt gatcggtcga aaaaactggc tattttctaa aagcttaaaa ggcgctagat
     2221 caaatggaat tatattgagt attatccaga cagctgttgc gaatgggtta aacatccgca
     2281 aatatttaaa tcatcttttt acagagattc ccaatctgtc ttcgatgaca cctgaagcgc
     2341 tacgcgctta tttgccatgg aatcagcaga tacaagaaat ctgtaaataa tataagccac
     2401 aaataacctg atttgagtgt atcaaatcgg gctatttgtg tttaggtacc attctatgac
     2461 acgcttacgc tcaaaactac tgaattatta agttatgtct aatcaaataa aaaaaccatt
     2521 ctcatatttg ctgaataatt agcatttaga gaatggtttt ttatctttaa aatctactac
     2581 tcaaaatctg catgtcgagc aaacatcgtt tctgagcttt cacctgtttt atccattaaa
     2641 gataacacta aactatcaaa ggtgagaaat gctaattgtt caaaacttga acccataggt
     2701 tgtgaaaaag tatcttcttc tcgttgattt tcatctttgg taatacctgg taatacaaga
     2761 acagaatctg ctaattgacc aatcgacgag tcttttttca tggtaactaa agcaatgctt
     2821 acaccacttt gatttgcttt ggccgctaaa ttttttaaac tacctgtttc acctgagcca
     2881 gaacagataa ttaataaatc tccaggttgc gaatgtggtg aactaatttc accgacaagg
     2941 ctgactgaaa aacctaaatg cattaagcga ttcgtaaatg cttgcatggc aattccagag
     3001 cgtcctgcac cagctagaaa gatgtgttta gcttgaacaa ttttctcact aaattgttct
     3061 aactctacag aattaatacg ttttgagtta ttttgtaatt ctaagataat cgcttctaaa
     3121 tagtttttat tttggtcttt attggtcatg atcttttact tcacttcctc taatgattga
     3181 tttagtttat caatttgttc ttgcggggta tttttcccat tgaagatgaa accgcctgat
     3241 acaaaaatat ccgcccctgc tttagaacaa actttaatcg tttgattatc aatcttgcca
     3301 tctacttcaa tatcatacgt ataatcattt tgtttgcgta ttacatctaa ctgttcgatt
     3361 ttaactactg tctcttgaat aaaatgctta ttctctgttc ctggattgat tgtcattact
     3421 aatacttgat cgactaaaga taatactggt tcaataaaac tgactggtgt tgcaggatta
     3481 aaagtaatcc ctacttttgc tcctgcttcc ttgactaact gaatgacctt cattgcgtgt
     3541 ttggtagctt caatgtggaa ggtaacaata tctgctcctg cttctacaaa tcttgtgaca
     3601 attttttctg gtgtatcaat catcaaatga acatcaaaca caacgtctgt tagtggacga
     3661 atatctttaa taacacttgg tccaaaagcc atatctggta caaaacagcc atccattaca
     3721 tcaatatgca aataatcaac attcttttct tttaagattt caaataactc tttaacttct
     3781 gcgataggtg catttaacaa tgatggagca attgtcattc tctattcccc cgtttattct
     3841 cccataatca tgagattgat tgttctagtt cgctctctta gttgatccca atcgacaaaa
     3901 tagacacggc ccatttctcc taataaaagt ttaccttctt gaacaattaa agtcattgag
     3961 ttggttccat aaatagatga acgtaaatgt gcatcggtat ttagcatgac ccatttttct
     4021 gcagggtaat tgggatcaga taatcttgtt ccaaaatcaa tgtgttttgg ccccggactg
     4081 ttgtattgat tttcactcgt catttttgga ataatcgttt ccattacatt gttgatgtct
     4141 acatgtaaat aatcatcacc aaaaaagttt gtgtcatgca tcgtttcttc aaaaaagagt
     4201 gagcaagttg tatgcgggct cgaaattact aacattccat tctcaatacc tgctgtatca
     4261 actgtatcta aaagttcttt tcgaatatca tgatacgaag gacgccctga aactgtttca
     4321 atagtcaaag tgtcatgatg aaaacgcata ttaaacacct aatctttctt ttacatctgc
     4381 ataaatggca tctgtaccaa tacctgttaa taagcccaag ccattcgtta ctttagcttt
     4441 aattacatca gggacaattg ttgtacttac tacagcatca tattcatcaa ttttatttag
     4501 ctcatcagca attttagatt gataaatttt cacttgatct gttaatcctt cttcttccaa
     4561 ccattttgat aattttgcta ctgcaattgt agaagtagca tgacctgctc cacaaataac
     4621 taataatttt ttcatattga tctcttcttt cttattttaa ttttatgatt aagctttctt
     4681 cactttgttc atgtagatag aagcacctac taaaacaact cctaatccac ctattccaat
     4741 tacaccaaag taagatgcta ataaaacaaa caaccctact ggccataatc cacataacaa
     4801 catagttact aatcctgctg tccccacatc gaaacttgct aatgtaaaca catcagtgat
     4861 aattggtgcc aaccatgttg ccatatataa tgttgtaaca acatacaacg aacatcctac
     4921 taatgatctc acaatatttc ctttaaaata aggaatcatt aaacagatgg cgaaagcaaa
     4981 gaatgctaaa tctccaaaag gtaatacttt atttcctgga agaattagtg ctagcaataa
     5041 tgagattggt acaagcaata gagaagttga cataatagtt ggatgaccca cagttaacgc
     5101 tgcatccatt ccaatgttta cttggcgacc atttaacttt ttattcgtaa attctttcgc
     5161 cgcttctgca attggcatta aaccttccat aaacattgct accattttag gcataatctt
     5221 catcactgcc gcagtcataa ttcctaagtt tccaattcct gctacgtcat agcctgccat
     5281 aatacccact acaataccga taattaggcc aataacaact gtgtcaccta aaacaccgaa
     5341 acgttttgtt aatgtttctg catctgcatc tattttatta aatccaggaa tacgatcaaa
     5401 aacccaattt actggtttgg caaatagtac tcctgataat cccatcatat gtgtgataga
     5461 gattcctgga tagccgaaaa attcgctaac agttttttgt gtacgatctg ctaagaacca
     5521 ttgtaaaatt actgcgacaa tcgcgccaat taagccaaac caaaagtttc ccgtaacaga
     5581 ttgagtaatc atacctagga aaattgcgaa ccaaaagttc caaatatcaa cattcaatgt
     5641 ttttgtccaa cccaaaacca caaatatgaa gttaagaatt aatgaaattg gaatcattaa
     5701 aattcccaat gatgtagagt aagccaaagg tccaccaaca cctacgccaa tatctaggat
     5761 attcaggcca ccaccgtatt gttcccccat ttgttgaatt gctgttccta aaccatctga
     5821 taataaactt aaaactaatc ctaaaccaat agaaccgata ccaacagtaa cgccggaaac
     5881 aaatgcttta ccaggtttta atccaaataa tatcccgatt acaaaaataa tcagtggtaa
     5941 aaagacgttt gaccctaatc caataaacca ttcaaaggca tttaatacaa tatccatctc
     6001 tctattcctc ctaataaact cctgcagaat gtactaactc aataaatgct tctttcgtgg
     6061 tacatgcaag taattcattt actttttctt cattttgaaa taggctaact aagttttgta
     6121 gtgtatcaat ttgttcatga ggttgtgaca ttgccaacat aaagactaaa cgtacaggta
     6181 tttcatgaga tttatctgtc atatctgaaa ataccagtgg ttcctttaat gaggcaaaac
     6241 agatttgaga gctatttaca tgtgcggaat ctgtatgtgg gatagcaaac ccaatttgat
     6301 ttacttctaa tccagttgga aattcttgtt cacggttggt gataccttct aagaaactat
     6361 ctttcacgca accagcgttt tttaattgtt cagacattac ttgaaacaat tctttttgat
     6421 ctttcacttc taattgaaat aaacataatt ctttatctag atacattttt attctccttt
     6481 ttgttgaaaa attcttagta atttttcgct atcatgaact tgtaacaagc gatcgaccat
     6541 ttctttttgc ccaataaatt ggtaaagttc ttcatatact cctctaatct cagatacatc
     6601 ttcttcagct aaactaacca taatgactaa tttgactttt cgatcgcccc aattaatcgc
     6661 attttttaat gctacaataa aaatgttgga ttcaatgacc attgatggat tcgcatgcgg
     6721 taaagcaaca ccactttcta aattagtatt ccccattttc tctctagaga agatagaagc
     6781 acgaaactct tcagtaacta atgaattggc ttctagttcg gaaatcatca aatccaaaca
     6841 ttcttctttg ctttccacct gtactcctgt ttttactaaa gatgattgaa caaatttaga
     6901 tatggtggga actttaaccg ctttaggaat cagttcattt tgaataaatc gcgaaggttc
     6961 ttctattaaa tatttagtat aagcttccat aatattcata taatctgttt tacttaccaa
     7021 tggagatacc cttactaagg gtataccacc tgcatcaaag gaaagagagg aaataatcaa
     7081 atcaacttcc ccaatttcag ctaaatgaaa cttatctaaa gttgatattt ctacattatc
     7141 ttttgctggt aaaaaccgtc gaactttatt gtaaattaat tgcgcagaag aaataccgta
     7201 ttggcaaata atgatgatat tatttgcttt ggactgatct tcaatagcaa tctgaaaatg
     7261 aatcaaaatt aaggaaactt catcgtccgt taaagtcaca tcaaattctt cttcaaggat
     7321 ggacatggcg taccatacta tgctaaagag ttctgaatat tgagatttga tattttctaa
     7381 taacggattt tgaattttaa tacctttttt taaacgaagg accattgctg gcacatgata
     7441 caataaggat tgatataatc gatcattttg agtcaaatca attttttcaa tctcactcat
     7501 tctttcaatc aatttatgaa cgctcatagc gtactcttca ttgctggttt ttaaattatt
     7561 tgtcacacga tgtgcaaata gttgacgaca tagatattct ttatctgcgt cataaaatgg
     7621 aagattcaat tttgccttca attcttcaat taaaccattt gccacaatgt acgtttccat
     7681 atagcgaatg ctattgaaga ggaattcttc ttcaacagtt aaatgatgac ctttcagtaa
     7741 acggttacat tggataacta aagtagaaat caatgaccga acatagtaat cagaaacctt
     7801 ttcagtcagt tcactgtaat cattgaatag caaatcatga atgacactaa taacttttga
     7861 atcaaacaac atatttaaaa cttgaaaaaa tgtttttgat tccatgtcat tcgcataata
     7921 gaatacaagt tgcttgattg ctcgttgaat tgcttgttca ttaccagtaa aactaattcc
     7981 ttctgcagat gactgcaact taacttctcc actgccaata attttattga taattctcag
     8041 gtcattgtat aacgatgttt tactaaccat aaaactttga gacaaatgag ttaaagaaac
     8101 aggcttatca ccaaacaaaa cttctttaat gatctcgaca cgtctttttt ctggagaata
     8161 aatatctttt tcaagataat tttctaccga tgaaatcaaa tgttcaacgt ttttcctatc
     8221 acctatgagt ttgataccca aagaaggtaa tcgctcaatt gttacttggt aattaactac
     8281 ataatcttcc aacaattcaa tatcttgata aatggtcttg gtcgacactg ccaatttatc
     8341 actaaaaaaa gaagcgggtt taaagtcttc ttgtcgactt aataatttta ataaatttac
     8401 atgtctttca atcaaacttg ttcacctctt aacgctagca tttgttacct cttttcatta
     8461 tttattctag cactggaacc cccttccctc aattacatct atgtcaataa tacttgtgga
     8521 agtttgttgt aaaggaaatc caaataatct taaaaaatga gaaacaaaaa aggagaaatc
     8581 cttaaaagag ctaagatatt ccaatagaaa atactcctta ttatggaagt aatatgaaat
     8641 attacaattg aaaaataaaa acaatataat ggcgtcacta aaattcgtat ttttttatta
     8701 ta
//
LOCUS       DAZKCY010000103         8696 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000103 DAZKCY010000000
VERSION     DAZKCY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.104"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(91..537)
                     /locus_tag="ACG8XC_002201"
     CDS             complement(91..537)
                     /locus_tag="ACG8XC_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFP9191876.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(564..740)
                     /gene="rpsU"
                     /locus_tag="ACG8XC_002202"
     CDS             complement(564..740)
                     /gene="rpsU"
                     /locus_tag="ACG8XC_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP9191877.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(902..1330)
                     /locus_tag="ACG8XC_002203"
     CDS             complement(902..1330)
                     /locus_tag="ACG8XC_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9191878.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1422..2282
                     /locus_tag="ACG8XC_002204"
     CDS             1422..2282
                     /locus_tag="ACG8XC_002204"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFP9191879.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(2374..2724)
                     /locus_tag="ACG8XC_002205"
     CDS             complement(2374..2724)
                     /locus_tag="ACG8XC_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9191880.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(2739..4466)
                     /locus_tag="ACG8XC_002206"
     CDS             complement(2739..4466)
                     /locus_tag="ACG8XC_002206"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFP9191881.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(4680..5606)
                     /gene="trxB"
                     /locus_tag="ACG8XC_002207"
     CDS             complement(4680..5606)
                     /gene="trxB"
                     /locus_tag="ACG8XC_002207"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP9191882.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(5751..7184)
                     /locus_tag="ACG8XC_002208"
     CDS             complement(5751..7184)
                     /locus_tag="ACG8XC_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9191883.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(7184..8587)
                     /locus_tag="ACG8XC_002209"
     CDS             complement(7184..8587)
                     /locus_tag="ACG8XC_002209"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9191884.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         2363 a         1947 c         1470 g         2916 t
ORIGIN      
        1 taagattctt tcttctagct gttttttagt tcttttttta cgaacacaaa aaactgcgga
       61 taatcactgt ccgcagttta aatgataaaa ttaagattgt aatagttctt tcacaatcgc
      121 attcacttgg tttccatctg cttttccttt gactttaggc ataattgcac ccataacttt
      181 gccaaattct ttcattgatg aagcaccagt ttgagtgatt gcttcttgaa caagttgtcg
      241 aatttcttcg tcggaaagtt gttccggcat atatttttcg actattacaa tttcactttt
      301 tactttttca gctaaatcgt cacgtcctgc ttcttcaaac tcatgcaaag aatcacgtcg
      361 ctgtttcatt tcacgagaca acaccgttag ttcttcttct tctgtcaagt cgtgaccttc
      421 tttaatctgc tcgttttgta tagatgactt gatcattcgg atgacttgta aagattcctt
      481 atctttcgct ttcatcgctg ctttcatgtc atcgtttaat ctacttagta gtgacacact
      541 accaactcca attcatcaca gaactagaat tttttgcgtt ttctagctgc ttcagatttt
      601 ttcttgcgtc ttacacttgg tttttcgtag aattcacgtt tacgagattc ttgtaaagta
      661 cccgcttttg acacggaacg tttgaagcga cgaagagcat cgtcaagtga ttcgtttttg
      721 cgaacgactg tttttgacat gttaattccc tccctccgag cttaaaaagt aaccgtaatt
      781 gttattgaaa tatatctcaa aaaacaagac tgtcctgtaa atattatatc taatgctgtt
      841 ttgaccgtca aggaaatctt gctttttttt aaaattatca aatgttattt tatttaaatc
      901 cttattgaca attttcacat cttccgaaaa tctcgaatct atgcccttcg atcgtgcatc
      961 cacctagctg ttcttcgaaa taattcatcg gacacatatg gatttctttc gttgttccgc
     1021 acactgtaca gataaaatga tggtgatggc cgattttccc ctgacagcaa tgaaaacgaa
     1081 acttcatttc tccatttaaa tcggtttctt ccagtaaatc gatctctgca aaatcatgga
     1141 gattccggta aatcgtatca taacttaccc ctttgaattc ctgattcata tagtcaaaaa
     1201 cttctttagc agatacgtag cggttacgct tcactaaata agttaacaat gcttctcttt
     1261 ttttagtata tttaaaaccg ttatttttca atgtttctaa tgattgttcg atcaatgtct
     1321 gttttttcat gacttatccc tccaaatcaa aatgattacg aagtacgcat tctagagtat
     1381 ggtataataa acataaaaaa aatgcaagtg aggttttatc catgacttcc gattctacag
     1441 aaatcgttac tttttttgtc atctccgact ctgctggaga aactgcgaca aaactagcgc
     1501 aagctactat ggctcagtat cctaccgttg agttcaacct attccgcaga acgttcgtga
     1561 cagatgaaaa aacattaagg caggcattgc aagatgcatt agaagaaaaa gcacttgtcc
     1621 tgcatacatt aatcaatcaa gaactgatcg agataacacg ttctttttgt gaagaacatc
     1681 aattatttag ttttgatgtg atgacgccac cgatatcaga aattgaacgt ttaactggta
     1741 tcaagccaat tcggcaacct ggtgccctcc atttattgaa tgagaactat ttcaaacgga
     1801 tcaaagcaat ggaatttgct gtaaaatacg atgatgggaa agatcctcgt ggttttttag
     1861 aagcagatgt cgttttgcta ggcgtatccc gaacttccaa aacgcctttg agcttgtttt
     1921 tagctaacaa aaatttaaaa gttgccaatt tacctttgat cccacaagca catattccca
     1981 aacagctttg ggagatcgat cctaaaaaaa tcgtgggact aacaaataac ccagatattt
     2041 taaataatat ccgtaaagaa cggatgcgct cttacggatt aaaccctgat actgcttatt
     2101 ccgatatcga aaaaattaga gctgaactcg attttgcgaa tgatctttat gaaaaactag
     2161 gatgtgtagt gatcaatgta gcatctttat ctattgaaga aacggcttcg cttattttga
     2221 atgctttaga cctagaagat catagttatt atggaactga aacaacagaa aaaagcgatt
     2281 gatcattttt gtctttttct taaaaaacac tggagtgtga caacgaaatt tcttcgttgt
     2341 cacactccag tgtttatcta tttgcaccta tcgttattga tgttccacat gtttgagtgt
     2401 ctggttcaaa atatccagta catgagaatc atcaagacta tacaaaatcg cttttccctc
     2461 gcgatgagac ttgacgacat gattttctct taataaacgt agttgatgcg atacagcaga
     2521 ctgctccatt tccactgctt gggtgattgc tgtcacattt tgttccccgt ctttcagata
     2581 tagcagaatt ttaagacgag tgggatcact caacattttg aataattgac tgactttctc
     2641 tacttttgat ttttgcgtgt ctggcaatcc attcacctcc aacttgaaaa aagactgact
     2701 tctaaagttt accacttttt ccatcagtct caagtttttt attcttctgt catctttttg
     2761 ataatcgctt cataagaggc tactttttcg gctgcgtctt ctgtagaact tccttttaca
     2821 ccaatataaa acttgatttt cggctcagtt cctgaagggc ggatagcaat ccagccattt
     2881 tcttccaatg tatacttcaa tacgtcggat ggcggtgttg ttagtttttc tacgtttcct
     2941 tcacggtctg ttctcgtcaa ttctttaaag tcttctgttt gaataacttt tgctccggca
     3001 aattctgtcg gtgcttcttt acggaatttt tccatcaaag cagagatttt cgcagaacct
     3061 tcttgtccgc tcattgtcac agaaatcgtt ttttctgcga aatagccata ggtttcaaaa
     3121 atcgtttgca agccgtcata caacgttttg ccttgttttt tatagtaagc tgctacttct
     3181 gctaataaaa gcaatgcttg gatcgcgtct ttatcgcgaa caaaaggttt aactaaataa
     3241 ccataacttt cttcgaatcc aaacataaaa gtatgtgaat ggtcttcttc aaattgttgg
     3301 attttttcag caatgaattt aaaccctgtc aacacattga acatggtagc accgtagtgt
     3361 tccgcaattg ctgtaggcaa ttcgctggaa acgattgatt tcaataccgc tgcgttttct
     3421 ggtaaagtgc ctcttgattg atgtgcttcc aaaatatatt gagcaaacaa tgctccgatt
     3481 tggttcccag tcaatacttc ataagaacca tctggcaaac gaaccgctgc gcctaaacgg
     3541 tctgcatcag gatctgttgc aatcagcaag tcagctcctt ctttttctcc aagtcgtata
     3601 gcatattcaa aagctgaatg ttcttctgga tttggcgatt tgaccgtagt gaaatctgga
     3661 tctgccacag cttgttcggg aactagtacg aattgttcga atccagcttg tttcaacgca
     3721 cgttcgccca acattttccc tgttccatga agtggcgtgt acacaagttt caaatctttc
     3781 cccattgttt caaccaattc acgatcaatc gtcactgctt tgatttcttc tagataagca
     3841 gcgtccactt cttctccgat gatcgtgatc aacccgctat gtttagcttc ttcgtcagat
     3901 aatacttcga cttttagcgg attgctgact tcacggacat atttcgtcaa tgcatctgcg
     3961 tctgcaggag gcatttgtcc gccatcttct ccgtagactt tatatccatt gtaagccgct
     4021 gggttgtgag atgcggttac catgattccc gcaaacgtct tcaaatgacg aactgcaaaa
     4081 gaaagctctg gtgttggacg cagactttcg aatacataag acgggatatt gtgatgtgct
     4141 agtgttttgg ctgcttccat tgcaaattct ggtgactgat ggcgtgagtc ataagcgata
     4201 gcgacacctc ggcgttttgt ttcttcacct tgtgtatcca tgaaacgtgc tagtccttct
     4261 gttgcttgtc ttaccgtata gatgttcatt cgattgatcc caggtcctaa gattccccgc
     4321 atacctgcag taccgaattc caacggtgca taaaaagcat cttcaagttt ttcaggatct
     4381 tcacccagat catttaattg tttttttaag ttttcttcta aattttcttc atgtacccat
     4441 tgttcataca cttgttccca agacatgaaa ataacacctc tttctgaaat atggttctta
     4501 ataagtatag cattcactta ttagatatga aagtatgagg accaaaaaac gcttaaatca
     4561 agaaaggatt cttttatcaa aaggtgttgg acgtgttcgc ataaatcaga ttcagtaaat
     4621 ttcacgcgaa aaaaaagagt aaaagaacaa ttgaacccgt tcttctactc tttttaaact
     4681 tatgctgttg tttcttctaa agactccaag tatttataca cttgttgtcc agcaataccg
     4741 ccgtcaccaa cagctgtagt gatttgtcgc aaatcttttt cacgaacatc tccgatggca
     4801 aaaacgcctg gcatattcgt tttcatttcg ttgtctgttg gaatccagcc agcttcgttc
     4861 gtcaaacctg cttctctaaa aggtgcagtt aatggatcca gtccaacata gatgaatact
     4921 ccgtctgctg ctaaatcaaa ttcattgccg gttttgacat tacgtgcttt cacacctgtc
     4981 actaccattt cgtttccaac gatttcgtca acgactgtat cccacataaa ggaaattttt
     5041 tcatttgcaa atgcacggtc ttggatgatc ttttgtgcac gtaattcatc tctacggtgg
     5101 acgatcacta cttcagaagc aaattgtgtc aaatagatgg cttcttctac tgctgagtcc
     5161 ccacccccga tgactactag acgtttgtta cggaaaaacg caccatcaca gactgcacag
     5221 taagaaacgc ctcgtccagc aaattcttcc tcgccaggta cgcctaattt acggtggata
     5281 catcctgttg cgataatgac tgtttttcct tcgtaacttt tatcttcaca gatgacttct
     5341 ttatggctgc cgtgatcctt gatatcttga acgattccat aagcattttc tgtcccaaat
     5401 cttgagacac tttcatacat cttataagct agatcggggc ccatgatcga atcgaatcca
     5461 ggataatttt cgatttctgc cgtattgttc atttggccgc caggggcacc ttgttcgatc
     5521 atcaatacgg ataagtttga acgtgaagca tataaagcag cagtcatccc agcaggacct
     5581 gcgccaataa cgattacatc atacatatgt agattctcct ttacttaatc aagtaagcat
     5641 actttacagc caatgcgaaa aaaagtctat tgttctgctt gcgtttctcg aatagcttta
     5701 ttatactcga aaaatccgct gaaaaacaat agacttttat aaatcattga tcaatcgttc
     5761 gaaagatttt ctccatttga atcaattact tttttatacc agttgaatga ttttttcttt
     5821 gaacgggcta atgtgccatt tccttggtta tcacgatcga cataaatgaa tccgtacctt
     5881 ttcttcatct caccggtacc agctgatact aaatcaatac atccccaagt cgtatagcct
     5941 aatagatcga ctccgtctag ttctactgca tccttcatcg cctggatatg cgctgccagg
     6001 taatcgattc ggtaatcatc ttccacataa ccattttcat ctggcgtatc gactgcacct
     6061 aagccgtttt ctacaataaa caatggtttt tgataacgat cataaatatc attcatcgtt
     6121 attctcaagc ctaatggatc aatttgccac ccccattcac ttgcttgcag atatggattt
     6181 ttgacagaag caaagatatt tcctgcagtc tgttccaaga gttcgttgtc ggtagtcgaa
     6241 actcgggaag cataataaga aaaagagata aaatcaacag tatgttcttt cagcaatgcg
     6301 gcatcccctg gttccatttc gatctgaatg ccatttcgct ccatttcttt caatgcataa
     6361 gcgggatatt ctcctcgtga ctggacatca atgaagaaat aattttctcg atctgatttt
     6421 ctagaagccc aaacatcttc aggattgcaa gtgtatggat aatatgctcc tgctgccagc
     6481 atacagccta ctttgttctc cggatctact tcatgagcaa tcttcgttgc gatggcactt
     6541 gcaaccaact catgatgtgc cgcttgatat ttgatttgtt cttcattttc tcctttttca
     6601 aaatacagcc ctgcgcccat gaatggtgca tgaaggatca tattgatctc attgaatgtc
     6661 agccaatatt tgacaagtcc tttatagcga ttgaagatga ctcgacacag attttcataa
     6721 aaaccaacga gttctctgga acgccacgca ccatattctt tgatcaaatg cattggacaa
     6781 tcaaaatgcg taatcgtcac aagtggctca attccgtgct tttggcattc tttaaaggca
     6841 tcctcataaa atttcaagcc agcttcattc ggctctttct catcccccat tggaaaaata
     6901 cgactccagg cgatggacat tcgataagtc ttgaacccca tttctgcaaa caaagcaata
     6961 tcttctttat aacgatgata catatcgatt gcaatttttg ctggataaaa atgatcctca
     7021 tcaaattcaa acattttttt ctttccggtg ataacaggaa aacggtcttt tccgattggc
     7081 acaacatcta cgtttgctag tccgcgtcca ccttcatcat aaccgccctc acattgattg
     7141 gctgctgtag caccgcccca taaaaaatct tttctgaatc ccattatact tcctccatta
     7201 tatgtgtatt acctgataat tctcttgcca gttcatcttg aacttcgatt ccttcttctt
     7261 ttaatcggct gattgtctcc ttgaattgcg gtaaatactg ctggtgtgca ataaacaact
     7321 catccatgat ccgttttgct gtctttcctg atcgtataag tggattgatc gtaaacgcct
     7381 gtaaagctgt cccataatca ccagtcacag ctgcttcgat cgtcagcaat tccatattct
     7441 tcattacttg caaccagcct ctttcagcag caggaagaga accaaacgct acatttctcg
     7501 ctccttgtgc gccaacatag gcagtgactt ctactacaca atctgccggc aggtcaggaa
     7561 cagctccatc attctttgta gataccacga tctgtgtttc tttatttcca tagattgaag
     7621 caatcgtttc acaggcagcg tctgaataat aagcgccacc tcgtttttgc aattgttcag
     7681 gtttatggtc taattctgga tctttgtata attcaaatag ctctgcttct gtttctttca
     7741 cttgctgcgc tcttgtacca atcgtttgat attcttctaa agcatgttcc agcatttctt
     7801 cttcacgata gtaatagcgg tggtagccac aagggatcat gttcatttgt tttaattgtt
     7861 ctttgaaaaa cgggatatca aagatatttt tcggtaatcc attctcttca tcgtataaac
     7921 gatcaatcaa ttctgccgtg cggtcatttc ctttattatc tgctactttg tgccaatgga
     7981 aatgatttag accggcaaac ttgtagatca gctcttcttt tttcttgcca agcattgctg
     8041 gttctgtcat catcgccatc actggcacat tgcataatcc aatcacttta tcccatttgc
     8101 catatctgat aactgcttct gtcaccatcc cacttgggtt cgcgaaattg atcagccaag
     8161 catttggaca taaacgcttc atgtcctcaa cgattgccaa aacgactgga acggtacgaa
     8221 aagctttgaa tatcccgcca gcaccatttg tttcttgccc aatcatccca taataactag
     8281 ggatccgctc gtctttgatt cgtgcatcta attgaccgac acgaaactga gtcgtaacaa
     8341 aatccgcatc tttcaatgct tcttcccgat ccagagttgc atagatcttg acttcatacg
     8401 gagaagcatc ccacatacgc tgagacatct gactaacgat ctcaagcttt tcttttcctt
     8461 cttctacatc tacgagccag atttctcgta tcggtaattc ctcatatctt ttgataaatc
     8521 cttccatcaa ctcgggggtg taactgctgc ctccaccaat cgtagcgatt ttgattcctt
     8581 ttgccatgtt ctcactcctt tactttttta cactttcatt atcttttttg taaacaaaac
     8641 aaacaataga aatctgtttc tttccagtaa catttacaaa atttactgtt ttctag
//
LOCUS       DAZKCY010000104         8590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000104 DAZKCY010000000
VERSION     DAZKCY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.105"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..332
                     /locus_tag="ACG8XC_002210"
     CDS             <1..332
                     /locus_tag="ACG8XC_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191885.1"
                     /translation="LLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSIS
                     LCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNF
                     YFYRNKL"
     gene            complement(458..1135)
                     /gene="nth"
                     /locus_tag="ACG8XC_002211"
     CDS             complement(458..1135)
                     /gene="nth"
                     /locus_tag="ACG8XC_002211"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP9191886.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1142..1840)
                     /locus_tag="ACG8XC_002212"
     CDS             complement(1142..1840)
                     /locus_tag="ACG8XC_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP9191887.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2108..3292
                     /locus_tag="ACG8XC_002213"
     CDS             2108..3292
                     /locus_tag="ACG8XC_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFP9191888.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3298..3741)
                     /locus_tag="ACG8XC_002214"
     CDS             complement(3298..3741)
                     /locus_tag="ACG8XC_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9191889.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3967..4254)
                     /locus_tag="ACG8XC_002215"
     CDS             complement(3967..4254)
                     /locus_tag="ACG8XC_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9191890.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4425..5777)
                     /locus_tag="ACG8XC_002216"
     CDS             complement(4425..5777)
                     /locus_tag="ACG8XC_002216"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFP9191891.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            complement(6049..6495)
                     /locus_tag="ACG8XC_002217"
     CDS             complement(6049..6495)
                     /locus_tag="ACG8XC_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9191892.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6731..7657)
                     /gene="cysK"
                     /locus_tag="ACG8XC_002218"
     CDS             complement(6731..7657)
                     /gene="cysK"
                     /locus_tag="ACG8XC_002218"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP9191893.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7797..8590)
                     /locus_tag="ACG8XC_002219"
                     /pseudo
     CDS             complement(7797..8590)
                     /locus_tag="ACG8XC_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
BASE COUNT         2335 a         1757 c         1427 g         3071 t
ORIGIN      
        1 tacttttaag cactactctt aaaggaaaat ttatggaaag aagctctcat gcaatgattc
       61 tcactttttt atggttgttg ttcaccagaa tctatatttt tctgatgagc ctgaaaccgc
      121 ccaaatccat tagtttatgt ttagttataa atggtatttt tttattatca cttatcattt
      181 ttattacacc tttagataga tacgtcactt atttatataa tcctttggaa tactggactt
      241 attttatagg tatattggaa ggcattattt tctacatcgg gtattttccc aataaaaata
      301 acaactttta tttttatcgc aataaattgt aataagttac catcctagct actgagtatg
      361 tacatttact ttttcatgca aaaaagataa acgaaacagc ttttctccat aataacaaga
      421 taattcatac atttatttgc aattaaagaa accacgtcta caggcgtggt ttctttttta
      481 gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat agcaaaggac
      541 atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa atcagtgtat
      601 gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct tctacttcca
      661 tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc acgtgggtat
      721 ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct gtctttctgc
      781 ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca tcgaaacgtt
      841 caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac aaaccaatcg
      901 tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg ggtgtcggaa
      961 aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc tgggcactta
     1021 agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca tgtgcttcgg
     1081 gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt aacatattcc
     1141 gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt tgagctgact
     1201 gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct tccacttgtt
     1261 gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg taattaaaac
     1321 tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc ggctgataat
     1381 ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc gggcgtccaa
     1441 attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt ttttcttcgt
     1501 attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg taaagattat
     1561 atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat ccatttgtta
     1621 cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc atagcaatca
     1681 tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt agaacaaata
     1741 aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga ttagatatga
     1801 ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct ccgctcaatc
     1861 acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat ttacttcact
     1921 gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt tttgatgcgt
     1981 atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag aatgttgaca
     2041 aggaaacaaa attactatac actatttttg tttccaagtt aacattattg cataaaggag
     2101 tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg tctctgtctt
     2161 attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa cgctgcctta
     2221 ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg tttatgctct
     2281 agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg ggcgtcatcc
     2341 gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg gtttaggaaa
     2401 ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag gagaaatctc
     2461 caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca aagtttttgg
     2521 atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag ggggtctatt
     2581 agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt tcttaaatgc
     2641 cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt cagtagattt
     2701 aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga tttcatccgt
     2761 tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc ttcaatctat
     2821 cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg gtttaagctt
     2881 ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc gattgctcaa
     2941 aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc tcgcctatct
     3001 attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg ggattatctg
     3061 ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat ccaattctgt
     3121 ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat ctctatcgcg
     3181 cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc ttccgacggt
     3241 attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat agtagcttca
     3301 tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg tgtcaaattc
     3361 ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt gatggagttg
     3421 ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga tttctttttt
     3481 attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt ttgttagctt
     3541 actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt taggatcagg
     3601 gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg caaattcttt
     3661 ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat attcttctat
     3721 tagcagttgt cttttatcca tttctacacc ttctttttct ttcattatat tgtttttttg
     3781 tttcctattc aacaaagata aaaatatttt gtaggaaaac ccaatctgaa accaagaaaa
     3841 aaaggggttg acaaagcttg tcataacccc tttttctgat taaaagatag taaagagaca
     3901 gtcatcggta attgtgatga ttttttgtca tcacttaagg atgagcggcc ttttcaaaaa
     3961 ctgtctttaa agatcatcat cagtttctag cacatatggc tgaccttcat atgtgtactc
     4021 tgtattcaat gtgccgtcat tttcatctga gtagatgcgt ctactgaaag gaaaacgatg
     4081 agaaacatta tctttggcgt catttgctgg catcgtgtaa tattcctctt gcgtttcatt
     4141 caactgagag agtacttgat ctgccaactc atcaatattg atcgttaatt tcttcgaagc
     4201 acttttataa tcttctaaaa atgcatcttg ttggacaaaa tattctttat tcatcttctt
     4261 tctcaccttt cttttcaaaa tcaaataaag ctgcttgttc ttttatgtaa cagctttatc
     4321 tttgctgctt attgtgacaa aaaattcgaa gaaagacaac taattgtact tttcacaaaa
     4381 aaagacagag ggtcgtgaca aaattgtgtc acaacccttt ttctttattg tgcagtttcg
     4441 atttcgccta gcgcttcttg tgcaagtagg ttcaagtaat tccatgggcg gtcaaaatgt
     4501 ggttggaaga agaaatctga aagtgcgaga tcttctacag tcattttatt ttgaacagct
     4561 aaagacatgg tatttgctga ttgtgtaatg tcgtatttgg acatgaactg agcacctacg
     4621 atacgattcg ttcctttttc atagactagc tccatcatta ctttttcagt agttggcatg
     4681 aactctggac gataattatc ttcaaataca gtcgcttgga cttctagatc gttcaaagtt
     4741 gcgctttctt tggtcacacc tgtcgaaccg atcgtccagc caaataggta aagtcctgaa
     4801 gttccttgag taccacgata cgccatttta tgatctgtca agttatttcc cacaagtaaa
     4861 ccttgacgaa cggcatttgt tgccaaagga atatagtttt gcgtgccact tggattgtag
     4921 ttgactactg cagagtctcc agctgcaaaa atgtctggat cacttgtctg catgtattca
     4981 ttcactttga ttgccccgtt tggcagtgta tctacttttc cttcgatcag ttttgtattt
     5041 ggacggaagc ccacacataa aatgaccata tctgtatcaa atgtatctgt tggtgtagct
     5101 acttgtttca cttttccttc ttcatctgtg ataaattcag taacattttc gcctagcgca
     5161 agagtaacac cgcggtctgt caattctttt tctaagatat ctgtgaatgg tttatctaga
     5221 tatttattca agatgcgatc taatccatca atcaatgtca cttgttttcc agattcgaca
     5281 aaagcttcta ctaattcaat accgatatat ccaccaccaa caattactac acgttttgct
     5341 tctttcgctt gtgcaataat ttcatttgct tgattgtagt ttttgcatag gagtacattt
     5401 ttcgcatcga ttccactaat cggtgggata ataggccatg aaccagttgt catcactaat
     5461 ttatcataag aaaccgtttc tgtttctcct gttttcagat cttttgccgt tactgtttta
     5521 ttttctgtat cgacgtctgt tacatcgtgt tccattttaa ctgttgctcc taatgaagat
     5581 aattcttcag gatttgagta gaacaagcct gctgggtcct ttactactcc gccgacataa
     5641 agtgcgatac cgcaagataa gaaagaaata ttgtcatttc tttcatatac tgtcacttct
     5701 gcttctggat gattttttaa aatacttttt acagctgcag tacctgcatg agtacagcca
     5761 attactacta ctttcattta tggtccctcc gattaacatt ttgtccttcg acaaaactac
     5821 tataacagaa ataaaacgga tacaacagtt taatgcttgt gaattcacaa aggtttgttt
     5881 gtagtttcat tccttttcaa gcataagaac ccaatatcta caagattctt attgtgataa
     5941 tattcacttt gatatctaag attgtgaata aaagacaaaa aaataaaaaa gctagcgttt
     6001 ttttacacag ctagcttttt ttaggtataa cacttataga aactgttttt acatttgcgc
     6061 cttttttgct gactctttac aatccggaca gagtccatac aattccaacc gatgttcatt
     6121 aatctcaaaa tcagtcagtc tgctcgcagc catttctata tcttcaagcc ctgggtaatg
     6181 aaaatcaaca atcttaccgc agttttcaca aattacatga taatgacgct ttgaactgaa
     6241 atcaaatcgg ctagaagcat cgccgtactt catttcttga acaaatccaa tcgaagtaaa
     6301 taagcgaagg ttattgtaaa cagtcgcaac actcatattt ggaaaccgat gttcaagtgc
     6361 tttataaatc tcatcagccg ttggatggga atgatgttca atcaaatatt ctaaaaccgc
     6421 gtaccgttga ggtgtgattc gaatgttctc tttttttaac ttttcgataa cctcttcgac
     6481 taacttattt tccatgtaaa caccccacgt tgttattctc gtcttatcat actattttta
     6541 cattttaaaa tccacttaac atgaaaagaa aattcaattt tattgtattt taatgaaaaa
     6601 agacgtaaaa cagtcgcgtt cactatttta cgtctctatt caaataattt ttctcatcat
     6661 aaatttttgt gtgttctttt catctttaaa agttttttac ataaacctat ctggagaaaa
     6721 accaagtgcc ttatatctct tggtaaaggg ctgtagataa ataacgttca ccattgtcgg
     6781 gaaccacagc taataccttc ttgccttttc ctaattcttt tgcgactttt agcgcggcag
     6841 caattgcagc gccagatgag atgcctacta aaatcccttc ttttcgccca acttctctag
     6901 ctgtttccat cgcttcgtct ccactaatag aaaggacttt atcatacaca gaagtatcca
     6961 gtgttttagg aacaaaacct gtaccgattc cttggatttt gtgaggtccc gcttctttcc
     7021 cttctaaaat agcagattct gctggttcta ccccgattaa ttcgattttc ggatacacac
     7081 gttttaattc tcggccagcg ccagtgatcg ttcctcctgt accgatacct gcaacaaacg
     7141 cgtccaagcc atttactcca aatgcttgat gtatctcagg accagtcgtc ttctcatgca
     7201 caagaggatt tgcttcattt tcaaattgta atggtaaaaa gtagccgttt tcttttgcca
     7261 aacgttctgc ttctctgatt gatccagaaa taccatccgc accaggagtc aaaatcaact
     7321 ctgcgccgta ccctttcatc aataaacgtc tttcgatact catcgtctca ggcatcacga
     7381 tgattacttt atatcctttt gcaactccta ccatcgacaa tccgattcct gtatttccag
     7441 aagtaggttc aatgatagta tcacctggtt tcagtagacc atcgtgttct gctttttcta
     7501 tcatacttaa agcaatacga tcttttacac ttcctcctgg attaaaaaac tcaagcttta
     7561 caaaaacatc tgctgaatct tctggtacga ttttatttaa tttgacgatt ggcgttttgc
     7621 caatcaattc ggtgactgag ttatagatag ctgtcatctt caattcctcc ttgtctttgc
     7681 tcttcttact tatcataata caatctgttc atttatggcg agtgattcgt ctttgactag
     7741 caaaaaaaag acaaagatat accatatatg gtagtttctt tgccttttta tctaaatcaa
     7801 acgtttagtt cttgaccaac taataaagaa taactggaca tagacatgcc atttttcgcc
     7861 attagatcat caacagacat gccaaatttt tgtgcaatat cccatagcgt atcacctgac
     7921 acgactgtat atgtctgacc attatttgta tttacatctg ttgttgaaga tgatgacgtt
     7981 gaagtcgtcg tagtagtcgt tgttgttgta cttgtggtag ccgaacctgt tgatggcgta
     8041 tcaaaacggg ttaaattata agtattaatc aatgagttca atttggcccc ataatttgga
     8101 tcggtagcat aacgacctgt caagtacgct gttgcatcat aaaaacttgt tgtattgctt
     8161 ttccacacgc cagcataatg ataatcgccc gtggaaaaac tggttgaacg aagaacattc
     8221 gcatggtctt ggaaggattc ccaataagaa gaatattttc ggaatggttc attcatcgtt
     8281 acccattgtc cgttacatat tctttggttg ccatataaac gacctgtccg ttatagcttc
     8341 ctttcacacc gaatagatta taatttggcg cactagctaa tccggaagtg ccatatgcac
     8401 tttccaacaa cgcttgagcg atcatgactg aagcatacaa gtcattactt gcagcaactt
     8461 ctgacgcaga ccagccgatt tgatcgataa acgcttgagg atcagcaact gtttgagatc
     8521 cttgttcatc tgcatgaaca tgttgcggta ccgctgtaac catcatcggc ccaactaaaa
     8581 gactcgttcc
//
LOCUS       DAZKCY010000105         8342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000105 DAZKCY010000000
VERSION     DAZKCY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.106"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(777..2408)
                     /locus_tag="ACG8XC_002220"
     CDS             complement(777..2408)
                     /locus_tag="ACG8XC_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFP9191894.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2422..4095)
                     /locus_tag="ACG8XC_002221"
     CDS             complement(2422..4095)
                     /locus_tag="ACG8XC_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFP9191895.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4108..4425)
                     /locus_tag="ACG8XC_002222"
     CDS             complement(4108..4425)
                     /locus_tag="ACG8XC_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9191896.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="ACG8XC_002223"
     CDS             complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="ACG8XC_002223"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFP9191897.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6087..6872
                     /locus_tag="ACG8XC_002224"
     CDS             6087..6872
                     /locus_tag="ACG8XC_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9191898.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6875..7567
                     /locus_tag="ACG8XC_002225"
     CDS             6875..7567
                     /locus_tag="ACG8XC_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9191899.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7841..8212)
                     /locus_tag="ACG8XC_002226"
     CDS             complement(7841..8212)
                     /locus_tag="ACG8XC_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFP9191900.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2559 a         1827 c         1431 g         2525 t
ORIGIN      
        1 attataacaa ttgggttaaa aagaaaatag atagtattct acctcatatt tatttgaaag
       61 taatatataa aagacgttca aattcgaacg cctctcgtta accaaatatt ggattcagaa
      121 tctagttttc atatataaac agagtatgat taggtgcaaa aattgcactt ttattgcact
      181 tttattttta tttatagtgt tttactgttc tgtatattgt tttaactccc ttgatagtat
      241 cgcattctgt tttatacact ttacgttgaa aacagagtgg gaataaagag ccaacataat
      301 atggaggggg aatccctata aatcaacatt cttcaatagc taaaatgctt gattttcatt
      361 aaaaataaga tggttcccta ccaaaagaga ctataatata agttaatttg tttcattctt
      421 atcataaaaa gaagttctcg aaaaacgaac tgttttccga gaactttttt ctatccatct
      481 aagtgctcaa atcatattca cgacggaatt ctaaggttac cctttcttaa acaaacttta
      541 tgttatctgc acaacttttt gcaatttttt cattgcttct tacgaaatcg ttgaactctc
      601 tttagcataa tacaaagtgt agatgaagtc tggtgcttta ctcgttttgg taaaaatgac
      661 tggaaatcgt ttctggttat ttttggcaaa ggcgaggtgc aggttcgatt cctgccttct
      721 acgctaacga tgtggctgaa gggaacctcc gatcttgaat cgaaacgatc cgcagtctat
      781 tgtttcacca atgaaacaaa atcaacgttc gtccaagcat ttcctgaagc cttgctataa
      841 accccaattt cacaaacacc tgttgtaatt ggaatgtctt tgattgtaat ttttgtccat
      901 tcacttgttt taacaaccgc agtatttagt tccgctccac cataattttt gatatataac
      961 tgattagtat caaactctcc cccagacatt acataggcac tcaatgtgta agttcctttg
     1021 cttatgccac taacattttg ataagctgat gcctcgtatg cttgatcctg ccaaaaagtt
     1081 agtttgtagt ccccaaaaaa agtgcccgct tccgttttga tcgtaccctt agcggttcct
     1141 tgttgaaacc aggtttgcca attggaagga ctggtcgttg gtccatcgct ttcgaaactt
     1201 gcatttttaa ctagatttat ttgcgcccct gcttgtttaa acgcacgcaa gcttggcaag
     1261 gcgtttccag caaaatcaaa catcgcttgg ttgtcccatt cgtttcccat agtggattga
     1321 acactgagat aattcatacc tgcttgtgtt gcccaactca catttccgtt gtaccaaaga
     1381 ggttcccaat agaagaagcc cgccccgcga ttattcggta ctgcctgaat ccgatcggca
     1441 agatccagca aatactctgc ttgtcctgtc ggtgttggtt catatccccc cgcagctgct
     1501 tcggcttcac caaaagcatt tggcatagta tcggcattcg ctgttgtatg cccataagct
     1561 gtttcaacta cgatcacttc tttaccatac cgttggctga tcgcattcat attggtttga
     1621 agttcattca ttgaaccgtg ccaatacgga taatacgaca ctccaattaa atcaaatgac
     1681 acacctcgac tcgtgatttc atcaaaccac caagtaaatg ctcctgtatc gccaccatca
     1741 gctaagtgga gcattactgg agttgtttta ggttgagtgt ctcgaacagc ttggatacca
     1801 gacttcaaaa aagcagccaa tcgatcaaat tctcccccgc cttctcccca agacttgcca
     1861 taaggccaga gcattccgct attcagttca ttgccgatct gaaccatatc aggataaacg
     1921 tccagctgct tcatttccga cagaacgttc tttgtatagt cgtacaatgc cgtattcatt
     1981 tcttcaaagg tcagaccttg ccatgcttta ggaagattct gtttcccagg atcaacccaa
     2041 aaatcactgt agtgaaaatc cagcagcacc ttaagtccca gattttttgc tcgttttgca
     2101 aggtcaatcg tcgtttccag atcatttgtc cctgctccat aactttctcc attactatta
     2161 taaggatcct gccacaaacg cagtcgaaca tagttgacac cattattttt taaaatctct
     2221 aaggcatctt ttgaaatatc gtcttcataa tattttgcac cagatttttc catatctgac
     2281 aggatcgaaa tatctgctcc gcgaataaat tcgctggcag atgcttcaac agtttttgaa
     2341 acagccccca aagcccctaa cacagctgca aataaaacag ctttacgcaa ccaaattccc
     2401 attttcatta tggctcctcc tttattgaaa ttggttcaga ataaactgaa cagctttttc
     2461 aggatctctc gttaatccga tatattctgc tccttttgtc gcaaccagct tgattcctaa
     2521 gcgatcggtg tcttctttga tatcatcata gtaagaattg acttgcggtg ctaagaccac
     2581 catatcaaaa tcctgcagca tttcgtaatg agaaccataa gcacccgcca ttgcactgat
     2641 cggtgctttc gattcagatg cacctttttt taaagcatta gctaacatcg cacttgtccc
     2701 tgcgccagca caaagtacca acacattgat ttctttattt gcatcgctga taacttgact
     2761 cgctgccaca ggtacttcct cacgtacatc ggcagaagca ccttcctcgg ttgcgatcac
     2821 ctctgctttt tgtgcttctt tctctactaa gacagcatcg tatgctttgc aaaatggtag
     2881 atagatcagg aagtcaacga cgattagaac cgctgctaga acaaaagcta agatacccat
     2941 gcccgttcct agaaccaaac cgatcggtgc tggagttgcc caaggcaaaa cgtacataaa
     3001 gctgttcatg cccaatacat cgacaaaaaa cttgaacagg caaacgttta caacaggtgt
     3061 taacaaaaat ggaataaaaa aataagggtt caaaatcatg ggtgccgcaa atagcaatgg
     3121 ttcgttgaca gcgaaactta ctggaataaa ggaagctttc ccaactgcct tcaactgctt
     3181 agacttagca aagagtagga agataaaagg aacaaccaat gttgccccag ttcctcccat
     3241 agttcctaca aaattcccta aacccaccgt cagtacttta gaagcttgtt caccggaatt
     3301 gaacaattgc agattggttt ccacattggc atagataatt gccgcaattg ccggctccac
     3361 gatcgacggt ccatgaacac cgacaaacca aaacagcgcc atcgctcccc agataatagc
     3421 gattcctaag tacccgtcag ctgctgtaaa tagcggctgc agcaccgtaa tgattgcttc
     3481 tgcaaatgag atttggaaga tttgtcgaat gcctaaatca attaaaacgg ccgcaaatac
     3541 tgaaaaagag aaaggaaaaa tatctcggaa ggtttgcgaa atcgttccag gaacttcttt
     3601 aggcatttta atggtaatat ctttcagcac acaaaactta tataaattaa ctgttaaaaa
     3661 tgcagcgaca aaggaagcca gtagtccctt tgtccccata tattccgttg aaaatccacc
     3721 ttcaatcgga gagacgctta acaacaggaa acttaccaca gaggccagca tcacagaggt
     3781 ggtgttgatt actttgtttt tcggtaagcg acgattcatc gaatcagcca agcagcgagc
     3841 agtggttgct gaaacgagta accctacaac acccatcgaa taattataga ctttccatag
     3901 ccatgtggag acagattcag gccattggta accaaaaatc tcaggaaccg cagcaatcaa
     3961 aatgaaaatg cttgaaaata aaatagctgg catcgctgtt aaaaatccgt cacgaacggc
     4021 tcctaaataa ctattacgtg agattttttc aaaaaatggt ttcccttttt cgatttgttt
     4081 aatgattcca tccatagcgt tcttctccta tccttgattc tgtctgtaca gtacaataaa
     4141 gtcttgaatc agatctctca gcaataaagt ggtcattaaa tgatcctgcc catggataaa
     4201 aatgaatccc atctccatct ctttcccaga tgcttcttcc gctaaaattt ttgtttgcga
     4261 attatgtgca agggttaaat tttcgtcggc ttctttcaac gccgaatcaa catgttcaaa
     4321 gtttccttgt cttacttctt tcaataattt caataatgta ctgcgagcgt cgcctgaata
     4381 tgcaacgatc tcaaaaccaa gcatttgtaa ttcctcttta ttcatctttt cttcctccat
     4441 tatcacttat taaataatcg ttttggtttc actgaccaac ttgtaccagt aagctgattc
     4501 ttttgggtaa cgcttctggg tttcaaaatc aacataaaac aagccatacc ttttgttata
     4561 cccatttgtc catgagaaaa gatccataag cgaccacaaa aaatacccct tcacatttac
     4621 cccggctgtt atcgccttgc ttaaggattc caaatagact ctcaaatagt cgattctcgg
     4681 ctgatccata ataatgccat cttcaaactg atctttatac cccatcccgt tctcagtgat
     4741 atagattttg ttgtaatgtg ggtaatcgct tttaattcgc agcaacaagt catatagacc
     4801 ttctggataa atcagccagt cccaatcagt tcgaggaatt ccttctttat aaatacgttc
     4861 tccgattccc ttcactttat aaacagaggt ccctttttca cccgtaccgt tatgatggat
     4921 tgcattttct ccatcgtaag ctttgacaaa atggcattgg tagtgattga tccctaagta
     4981 gtcattacgc gtggatgctt ttttcaattc aacgaaatct tcttcaggaa aatgatagct
     5041 ggcctgattt gcttcacata tctcatcaag agcagtcaag gtttcagttg aataatagcc
     5101 taaataggta gcatctaata agaaacggat cgacaatgca tcgtctaaaa aggcagcatg
     5161 cttatcttcc ggcgcgtctg ttgccgcata tttcgtctct aatgagtgaa ctacaccaat
     5221 ttcacccgga agttcgtttt ctttgaaata gttcactaca cgggcatgag caaccatcat
     5281 attatgaaga caggcgacga tctttgtaaa atcatatttg attcctggtg ggaaaacacc
     5341 taacaaatat tgattggttg caactggata gatttcatta aaggtactcc acaccttgac
     5401 ttctttgaat tcatgaaaac aaaaaatggc ataggaaaca aatgcttcta ttgtttctcg
     5461 attcaaaaaa tctccatggt caaacaaacg tttaggggta tcaaaatgat gcaacgtcac
     5521 aaacggcgtt acgtttcttt tcttacattc ttcgaacaca cgatgataaa aagcgattcc
     5581 ttctggattt ggtttcccag ctccatcagg aaaaatgcgg ctccaagcaa tggataatct
     5641 tagaccattt acaccaaaac gttcacacaa ttcaatgtcc tttgcatatt gatgataaaa
     5701 atcacttgcc ggatcgggac tgaatcgccc ttgttcctct aaaaagtcat cccaagcaac
     5761 agcgcctttg ccaccttcct tagtggctcc ttcaacctga taggctgctg tggctccgcc
     5821 aaaaataaaa tcttctggta atttaagcat gtacatctct cctcaaatct ataatcagtt
     5881 ttcgctcttt ctaactgatt gcattgtaaa cgcatccaaa acatttgtca acaaaaacaa
     5941 acacaaataa tcataaaaac cagaaaacag caataaaatc tattgtaaca aacataaaaa
     6001 ccaccctaaa tgtttacttt tgttcgaatt agattgactg ttgtccgttt atatgataga
     6061 ttatccttaa attagaggtg aagacaatgt tgaaaaaaga aagattgctt actatcgtag
     6121 aaatggtcaa taaaaaagga atccttaccg ttaatgaaat catcaatcaa ttagatgttt
     6181 ctgatatgac cgttcgcaga gatttagatg aattagaaaa atcgggcaag ctgctgcgtg
     6241 tccatggagg ggctcaaagt ctttcctaca ctttagatca agaactttct cacactgaaa
     6301 aagccactct gcagattcaa gaaaaaaaag aaattgctgc cacagcagcc tccttgattc
     6361 acgagggaga aacgattttc ttaggaccag gtacaacaat cgaactattg gcgacgcatt
     6421 tactgaacaa gaaaattcgg atcattacaa ataattatcc agtttttgat attcttgctc
     6481 aagcagatgg accagaaatt ctactgattg gcggtgattt ccggaaaaat accggcgcat
     6541 ttgtcggacc gatcaccaac gacaatttaa gaagactaaa gtttacaaaa gcatttatca
     6601 gttcaaatgg cgttcacaac gaagcgatct ctacttacag taccgaagaa ggagaagcac
     6661 agcagatcgc tctaaataat tcccgtaaga aatatctact cgttgataat aaaaaattca
     6721 atcgagatga tttttatgtg ttttataatt tgcatgattt cgatctgctc attacggatc
     6781 gccagatctc gcgggaggtt caagagcact atgcacaata tgtcgatctg caaattgctg
     6841 attcaaccga aaataccact ataggagcgt aactatgaaa gggattatat ttgatttcaa
     6901 cggaacaatg tttttggact ctcatctgca tgaagctgct tggctgcata tgatacatga
     6961 tcatacaaaa gatggtttat cagacgagga tattttaaaa aatattcatg gtcggacgaa
     7021 tacggagatt ttgaaacatt tcatttcaga gcatttgacc gcctctgaga tcgccgtatt
     7081 gtcagaagaa aaagaaagct attatcgtat gctttgcttg caaaatgaaa acgagcttca
     7141 gttgacaaaa gggcttgaag cgactcttga ccagctgact gaccaacaga ttccattaac
     7201 gattgccacg gctactgtca aagagaatgt tgctttttat tttgatattt tcgatttagc
     7261 ccgctggttt gactgggaca agattgtcta tgacgacggc tctttccctg ggaaaccgca
     7321 accggatatt tttcttaaag cggcagacaa actgagctta gctccggctg attgtcttgt
     7381 tatcgaagat gcttactcag gattacttgc tgccaaacgt gcggggatcg gaacaattat
     7441 tgcaattgat ccattcggta aaaacaaaac agtatttgaa caggcacagc taaataaaga
     7501 tggaaccatc aaagatttta ctggtttttg gacagagcac ctgtctgcca catccgttaa
     7561 ttcttaaacg attcaactaa ctaccaaaga aaagcaactg aaatgcacac ctccttttat
     7621 aaaaaactgc cagtgaaacg acatgaaaga tacactgcta tatccttagt gaaatatagc
     7681 agtgtatctt tgactttttc attcttttaa ccatatgacg cgttcatctc tatctataat
     7741 taacattaaa aatagtgttt tcacattatt tctcaaacca atcttaaaaa tcatcaggag
     7801 tacttttttg atatactttt attaatgctg ggcgtaatgg ttaaaagatt tccactagtt
     7861 cctctccacc aagctgagtt gaaaaatgct tatctgtaaa caacatagac aaagaggaaa
     7921 taaacgtttc cgtatctttc tctgtcccaa ggatattaat taattttcct actaggtaag
     7981 tatcattaaa cttaagatct gccttcacat caaaagtttt gataaaagct tttatcatgc
     8041 gtacaatttt gtctttatcg ccataattga tttcaccact catatcaata tagtcactaa
     8101 tccctgaaga aataatggca actgcttctt ctttcgcctt atcagataat tcatttaatg
     8161 actgggattg attgatgact tcttgagttt tatctgttac gtaatctctc attatcctat
     8221 cggcatactc cgcaaagtaa tcttgaaaaa attcttctac agggtaacca acttcgtttt
     8281 cccaaccagg agaaatttca ttatcagtac ttattaaatc tattaaaaac caacccgttc
     8341 tc
//
LOCUS       DAZKCY010000106         8249 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000106 DAZKCY010000000
VERSION     DAZKCY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.107"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..474
                     /locus_tag="ACG8XC_002227"
     CDS             <1..474
                     /locus_tag="ACG8XC_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9191901.1"
                     /translation="LQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDS
                     SENHYNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIA
                     LQEQDFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            504..626
                     /locus_tag="ACG8XC_002228"
     CDS             504..626
                     /locus_tag="ACG8XC_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFP9191902.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            644..2167
                     /locus_tag="ACG8XC_002229"
     CDS             644..2167
                     /locus_tag="ACG8XC_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFP9191903.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            2191..2430
                     /locus_tag="ACG8XC_002230"
     CDS             2191..2430
                     /locus_tag="ACG8XC_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9191904.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(2623..3084)
                     /locus_tag="ACG8XC_002231"
     CDS             complement(2623..3084)
                     /locus_tag="ACG8XC_002231"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9191905.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3279..3497)
                     /locus_tag="ACG8XC_002232"
     CDS             complement(3279..3497)
                     /locus_tag="ACG8XC_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9191906.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            3615..5231
                     /locus_tag="ACG8XC_002233"
     CDS             3615..5231
                     /locus_tag="ACG8XC_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9191907.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(5472..6812)
                     /locus_tag="ACG8XC_002234"
                     /pseudo
     CDS             complement(5472..6812)
                     /locus_tag="ACG8XC_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7250..7396)
                     /locus_tag="ACG8XC_002235"
     CDS             complement(7250..7396)
                     /locus_tag="ACG8XC_002235"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191908.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHIKKEIVVRP
                     LQIY"
     gene            complement(7435..7629)
                     /locus_tag="ACG8XC_002236"
     CDS             complement(7435..7629)
                     /locus_tag="ACG8XC_002236"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9191909.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(7539..7871)
                     /locus_tag="ACG8XC_002237"
     CDS             complement(7539..7871)
                     /locus_tag="ACG8XC_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9191910.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         2686 a         1329 c         1625 g         2609 t
ORIGIN      
        1 ttacagagag aaatcaaaag tattaaccct ttatccgata ttcgaatgtt cacacatagt
       61 gaaacgattc aaaaaaagga tttttataat ttgaaaaaat ttaatcatcc cttagttttt
      121 gaaagtgatt catcagaaaa tcactataat ggcaaatact atcatcaaca ccatcaagta
      181 catgagttta aatcaattct cttaacttca gatcaagaca taaaaaaaga ttttttactc
      241 caatggattg actggctgat tttaaccaac caagggagcc tgtatcgttt taaaggattg
      301 attgctttac aagagcagga ttttatagtt gccatgcaag aggtaaacga acaggttaat
      361 tttcaataca ccactcgaca aaaagaccaa accactatta ttctaatcgg aaaaatgttg
      421 gatgaagaaa ggatcaaaca gagttttgat aaattactgg cggaaagcaa ataatgataa
      481 gtagtgattg ggggtaattt ctaatggaga aaacggatat atctagtgca tatcgacgac
      541 ttaaaagccc gaatattaaa actagaaaaa gggcattaaa aattatcaaa gaacataaac
      601 gaaataaaat gaagaaatta gcataattaa ggaaggaaaa agaatgaaga aaatcagtgt
      661 aggacttatt gggatagcag ctctaggatt attgggagct tgctccagca ctaatgatgc
      721 aaaagtatcg aatgataaag atggaaaatt agaaattgta acaacattct atccaatgta
      781 tgatttcacc aaaaacatcg tcggcgatga agcgaacgtg gatttaatgg ttccagcggg
      841 atcagaacct catgactatg agccctcagc taaggatatg gcaaaagctc atgatgctga
      901 tgtttttgta tatcataacg aaaacatgga atcatgggta ccaaaagcta aagaaagttg
      961 gaagaaagct gggcctaatg tagtagaagg aacaaaagac atgattttat tgccaggtag
     1021 tgaagaagaa gatcatgatc acggagaaga agatcaccat catgaactag atcctcatac
     1081 gtgggtttca cccaaaatgg caattaagga agtttcaaat atcaaggatc agttagttaa
     1141 attgtatccg aaaaaagcaa aagtatttga gacaaatgct gaaaagtatt tgactaaact
     1201 gaaacggttg gatgctgact ataccactag tcttaaagaa gcgaaacaaa aaagttttgt
     1261 gacgcaacac gcagcctttg gatatttagc tttggattat ggattgaaac aagttcctat
     1321 tgctggacta agtcctgaag aagagccatc ttctggtcga ttagctgaat taaaagagta
     1381 tgttaaaaaa aataagatca attatattta ctttgaaaag aatgctaatg ataaaattgc
     1441 aaaaactttg gccaacgaag ctgggataaa attggaagta ctgaatcctc ttgaaagtct
     1501 tacaaaagaa caaatggata atggtgaaga ttatgtttca gtaatggaag acaatctaaa
     1561 agcgttagaa aaaacaacta tggttgctgg tgaagaggta gttccagaaa aagaagcgaa
     1621 ggatgaaaag acagttgcta atgggtactt taaagatgcc gatgtgaagg atcgcgaatt
     1681 gagtgattat acaggcgaat ggcaatctgt ttatccgttg ttaaaagatg gaattttgga
     1741 tgaagttttc gattacaaag caaaaatcaa taaagacatg acagctgccg agtacaaaga
     1801 ttattatact actggctata agacagatat tgatactatc aacattaaag acaacaccat
     1861 tgattttgta gtcaacggtg aacatcatca atacacatac aaatatgttg gatacaaaat
     1921 tttgaattat gaaaaaggaa accgtggtgt tcgttttaat tttgaaactg atgatgcagg
     1981 tgctggtcgt tttaaatata ttcaatttag tgatcatgga attgcaccaa gtaaggcaga
     2041 gcatttccat attttctttg gtggagaaag tcaagaaaaa ctctataatg aattgcataa
     2101 ttggcctact ttctatccag cttcattgtc taaacatgaa attgctcaag aaatgatggc
     2161 tcattaaaat tcaatttttg gaggaaatac atggtaagaa aagtattatt gtctatggat
     2221 tccaagaata ttgatgctta tcggcgttat cgatttgcaa agcaaatgag attaaatggg
     2281 agtacattag ctgatattgc tgcaacttta catgttagta ttgacacagt tcatagagta
     2341 ttaaaacggg atcctgattg ttgttttatc aaggtcaata ctggaagaag tagacgttta
     2401 gcaaaacggc ataatagctt ggccaattaa ttgataatac cagagtagat catttttccc
     2461 ttatttggat ttgatctgct ttttattatg tcttgaattg tcaaatcaag cgcaacactt
     2521 tatcaaagaa gacttcataa ggtgttctcc aatctaaaca ctttctaggt ctgttattca
     2581 gtttttcaat catctgattt atcgttgagt tatctaccgt agctatctct ttcgattttg
     2641 gcaagtattc tcgaatgagt ctattcgtat tttaatttgt tcattgttgc caagggcatg
     2701 aggatctgga aaataaaagg gcatattatt caatgcttct gtaactgcga aatgattgga
     2761 gaattctttt cctctatctg gagtgatcgt tttgaccgcc tcatttggaa tactgcttaa
     2821 aagttcaatc attttctcgc tcacataagc ggctttcttt ctggggacct tctttcctgc
     2881 taaaaggaag catgattttc tgtctgtcaa agtcactaaa caagtaccac cagttttgcc
     2941 agctacaata tcggctttcc aatgaccaaa atcacttcta tcatctgcgg attttggacg
     3001 gccatggatc gaatgagtaa ggcaaatctt tccacgagac tcgacatgat tttttgtatg
     3061 tcttgttttt cctcgatggt gcaatgaacg gatcaggcct ttattgccgt gtgagagggg
     3121 ttctgtatcg aaaagatcat tgcaaattgc acgttagatc gtgataaaac taattgaaaa
     3181 gtccatatgt tcatgcttca aacgattcga gatttcctct ggcgaccact gaaaatcgac
     3241 aaataaatga cgaacaattg accaaactca cggatcattt agaatgtgat aacgtccgca
     3301 tttttttctt cgataagcat aatttttttc cgctttggaa ggtgaatagt ttttttcttg
     3361 tttcttctca gttcatgtaa aatagtaaac gggctatgct gaatgtcgtt gccaattata
     3421 ctaatagaaa atccttcttc gtgaagcaga aagattcttt ctcgttcgag tatagtaaga
     3481 tgacgatact ggctcataat tattttcctt ctaggagtgt tctcgacaaa cttatactag
     3541 ttggatatcg attatgactc atttttatgt gttgcactta aagtgtaaat tcaaggtata
     3601 gaaagaggtt ccctatgaaa aaaataccta ttacaatctt aactggctat cttggttcag
     3661 gaaaaacgat acttctaaat catattctgt acggtgatca cggaaaggaa attgctgtca
     3721 ttgtaaatga cataggggaa gtaaatgttg atggggtatt agtcgacaag aaaggtttta
     3781 aaagaactgg agaaaaactt gtttcgatgc cgaatggatg catttgttgt acattacgcg
     3841 aagatttgat tgttgaacta gatcaattag tgcaattttc tgacttgaat caaattgtta
     3901 ttgaagcttc aggaatcagc aaaccaattc caattattca agcaattata ttagctaaaa
     3961 accctttaga cattgatttg acccaacgat tagagattgg tgccattatt atagtagtgg
     4021 atgtaaaacg tttttgggat aatttcgaaa cgggtgaaaa gattcaggaa agaaaagctg
     4081 aaggtaatgt agttgaagaa gaacaggata ttcgtggctt gttaattgat caattcgagt
     4141 gttgtaacat attactttta aataatagtg acttagtgtc aaccaagacg atttacaaaa
     4201 ttaacggtct aattgctaag ttacagtcca atgccaaaat aattaggaca attaaaggtg
     4261 aagtagactt atcgaaaatt ctaaatacaa atctttttaa tatagatgag acttcctatt
     4321 cagtgggctg gttcaaagag ttagagcttg gctatcaaaa tcatattcct gaaacagaag
     4381 aatatggtat ctcaagtttt atctatcgaa atcggttact ccttgacgca caaaaatttg
     4441 cccatcttac ggataaaaat tttcctggca atattacaag agcaaaaggg tttctttggt
     4501 ttgcccaaca tagtgatttt tcgacgatct ttgaaacatc gggtagctat aaaagtattg
     4561 aatcattaaa ttattgggta gctagtttac ctaaaattga gtgtcaaatg attatacaaa
     4621 aaaatcccga attgaaggaa gcctggcatc cgttgtttgg tgaacgcatt aataaactag
     4681 tttttattgg tatgaagatg gatcaacaat gtattaagga acaacttgag ggttgtttaa
     4741 ctgattctct taatgcagat aatttccgaa aaatttcacc atttccatgg aaaaagtcta
     4801 atatgtttag ccagatatcg ttaatgaaat ttttaagaaa agctggtgtc aagataggcg
     4861 atgtcataat tattggcatt ttgattatta cctcttttat tcccttattt gtattctcta
     4921 tagaaaagga gaatgaaaca actgaagatg ctatcaaata tgccatcgtt cgaattgatg
     4981 gcaaggaaat ggatcgcttc gatttggata agatcaatca taaactcgtg aattatcatc
     5041 ctgcgaaggg acaatataat attgttgaga ttaaagatgg aagaattcga gttaaagaag
     5101 ataatagtcc tgatcaaatc gcggttagaa ctggatggat ctcaaaaccc ggtcaaacga
     5161 gtatttgttt accccataag ttagttatta gtattgagaa aaaagaaagc acagattact
     5221 acatttacta atctggaaat agatgaggag gtttttaata agaaaaagat attattatta
     5281 ataatagcag gggctttatt aagtgtgtta agcgcttgtg gacaagaaaa agaagcggtg
     5341 acaacaggca caagtatctt taacgggacc tgcaatggtt tagttaacag tcggttagat
     5401 tacttatttt ggctcttcgt caatgagtgt tggtgaagaa attcaataga ataatcgaga
     5461 tactcacacg gctagacagc tatcttgtct agccgtgttg ttgttctggc tttagtcagt
     5521 tgggtagtat tcttagtttt cttaaaatat tttcctcgat ataaaaagat tcgttctcgg
     5581 tagttttgaa agtttctata gccataggca attcgtttta gtactttgat atggttattc
     5641 aaacattcta tagggccgtt ggaataggga acgcgcaacg ctcgtttgat ttctgtttgg
     5701 tatttcttga aggtcgtgaa cactggttgg tagtgctctg gcaactcctt gtagtcttct
     5761 tttaggagcg cgacgaattc aggaacatct tgtcttctga taacggaaag aaaatcctgg
     5821 tagacttcgt agcctcgctt taaggaatca tcataagata atagccgatc gactatctct
     5881 gcttctgtca gatgatctcg aaatgacgga cgccagtagc gatgctgacc tttcaattcc
     5941 cacgcattct tttgaagtag tttccagtat ttctttaagt gcttcgaccg acgatcccct
     6001 tttttaaagg tcttcattac ttgaattcgg tggttggtaa aagcccgacc aatgtgttgg
     6061 ctaatatgaa agcgatcggc gacgattcgg gcattcggaa aacactcttt caccaatgtg
     6121 cgatagggtg tatagaaatc tgaaaccact agacgcactt gttcacggac ttttataggg
     6181 tagcgcaaga aatagtcgcg taaaggattg agttgccgat tttccacaat atcaatcagt
     6241 tgatgggtct tgccgtccat catgacaaag ctcatggctc ccgagacatt tttcactgat
     6301 ttgaattcat cgaaacacac cacttctggt aacagctggc tcaaagaaat ggttttaggc
     6361 cgaaggctgc gaagtattcg aatgacagtg gtcggagaaa tgtgcttcat acgtgcgatc
     6421 aaagacatag aaagaggttc gcttaatagc tcaagaatgg cttgtttgac tctgcgagca
     6481 atggagcagc gacgatcact aactgaatct tctgctaaaa aagtctttcc acagtgttta
     6541 catttgaagc gctgtttgtt aatccgcaga atggtcttgt agttactgac atcattcaat
     6601 aagatggata cttttttcca gccctattta atgatcgttt gttcattttg atagtggcga
     6661 tgaaagcatt cctccggttg ataggtcaat gtgccatcaa aagcaaaaca tgtttcgtca
     6721 ttgatgcgct ctttgcgaaa ggcatcctca tgaaaggtaa gatttaggtc tagaatatct
     6781 agtaactctt tggtaaactc tgaataggac atcttgaaac actactttga atggtttttg
     6841 tcgactttat tctaaaggat tttaaggtgt ctgtttgcgt ttattagaaa aaagaaaaag
     6901 tattggcaac agaaggcgtc accaacacta gatattatag atcccttatt ttcttcgctt
     6961 caaattgtca aggagtcatc ataaattttg tatggattct tttgtttggg tagtagtctt
     7021 ctaaataatc ttggattaac accttcatct ctattttact tttatatttg ttggagtcga
     7081 ttaattctca tttgattgat cgatgaaacg cttcaattac tgcattgtca acaggagtcc
     7141 cagtttggct cactgaatgt gtgatgtggt ggtcgttcaa cagctcttga taaataaaag
     7201 accgatgctg gctacctcta tcggaatgaa ttaccgtagg caattctcct taataaattt
     7261 gtaatggacg gacgacaatc tcttttttta tgtgtgaatc taccatatag taacgtagaa
     7321 tacgagttgc taagtcaata taggtggaca aatagaggcg accgtcatta catggaatgt
     7381 aagtaatatc catcacccaa acagcatcaa tttggttctg ttcaaattat cgattcaaca
     7441 tttttttacc atttctttga ttgttttttc tctaccatga gaagatttgc gtcgatgaaa
     7501 tcctcttgtg cataaattca tttcacgcat aagaacagct acaacacgtt tattgatttc
     7561 gattccttca tcgtaaagtt tttgagtgat ccctggagat ccgtatcgat ctttgtgttc
     7621 gtagaacact cgtcttcctc ttttagtcgt ttattttcct gttcaagaat gtagaaatct
     7681 gctttatcag gtaatctttt tccttgtcca gcaaaagcat ttttcccgta tcgacgatat
     7741 tctgaaaccc atcgggaaat tgttggacga gagatattgt attcttcagc aattttcttg
     7801 gtagttactt ttttctcaag gatatcctgt gaaatcttta gtttaaattt acgatcatat
     7861 gtttttctca atagagttca ttccttccgg taacgtaact gatagtcaag aaaagtgtat
     7921 catagcccaa cttagtatca gggtttgtca actaaactgt ggaagttgat agataagagt
     7981 ttttaacaac caaaattctc ttgactattt ttgaaatcag acaaattttg attggacaca
     8041 tagttgaata aatctaccgt cgttacggaa ggtaaatcgc atgcttttca attctagagg
     8101 agaagtcctc gttgaagtac catagcccag ttctggattc gacctcctgc ccacttcata
     8161 cgtaaataaa gtaattttag gagtgagact gtgacaaaag ttatttttaa gctaaaaacg
     8221 aattaaaaaa cgttgataat taaaatcat
//
LOCUS       DAZKCY010000107         8226 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000107 DAZKCY010000000
VERSION     DAZKCY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.108"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..481
                     /locus_tag="ACG8XC_002238"
     CDS             8..481
                     /locus_tag="ACG8XC_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFP9191911.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            490..717
                     /locus_tag="ACG8XC_002239"
     CDS             490..717
                     /locus_tag="ACG8XC_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9191912.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1353..1724
                     /locus_tag="ACG8XC_002240"
     CDS             1353..1724
                     /locus_tag="ACG8XC_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191913.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1980..2222)
                     /locus_tag="ACG8XC_002241"
     CDS             complement(1980..2222)
                     /locus_tag="ACG8XC_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9191914.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2254..3156)
                     /locus_tag="ACG8XC_002242"
     CDS             complement(2254..3156)
                     /locus_tag="ACG8XC_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9191915.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3169..3357)
                     /locus_tag="ACG8XC_002243"
     CDS             complement(3169..3357)
                     /locus_tag="ACG8XC_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP9191916.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3371..3934)
                     /gene="amaP"
                     /locus_tag="ACG8XC_002244"
     CDS             complement(3371..3934)
                     /gene="amaP"
                     /locus_tag="ACG8XC_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP9191917.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3972..4865)
                     /locus_tag="ACG8XC_002245"
     CDS             complement(3972..4865)
                     /locus_tag="ACG8XC_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9191918.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4943..5866)
                     /locus_tag="ACG8XC_002246"
     CDS             complement(4943..5866)
                     /locus_tag="ACG8XC_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9191919.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5915..6265)
                     /locus_tag="ACG8XC_002247"
     CDS             complement(5915..6265)
                     /locus_tag="ACG8XC_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9191920.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6298..7200)
                     /locus_tag="ACG8XC_002248"
     CDS             complement(6298..7200)
                     /locus_tag="ACG8XC_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9191921.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7193..8050)
                     /locus_tag="ACG8XC_002249"
     CDS             complement(7193..8050)
                     /locus_tag="ACG8XC_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9191922.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         2252 a         1587 c         1393 g         2994 t
ORIGIN      
        1 gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta taagtgccgt
       61 atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa aaggattcaa
      121 ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc tcctagggat
      181 cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc aagctttttc
      241 cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta ttggtttcgc
      301 cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg ctccaggttt
      361 gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat taacaagcgt
      421 gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt tgacagtgtg
      481 aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa aaaaatgagt
      541 gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat tctaaaaaat
      601 ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt tttggagtgt
      661 caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga agaataaaat
      721 aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga gacttttaga
      781 agttatttac gtctattatt cagatttttt atttcgattg aataccgttt tatctataaa
      841 taagatatgt actgactcat acaaaggggg ccttttcttc tccatgtact atcacttttc
      901 ctgatacaac tggtttcctg ttacgttgta gttggatctg cttttagtat aatactttat
      961 tcattccacc agactgatag cagtttgagc agtctaaata atcatgtagt attacaggcc
     1021 attctgtttc ataccttgaa gtgcttaaaa cttcagctaa tggcccattg tgttaactct
     1081 tactccattc ttttttagta agctcccata tatactgtaa agccttctat atattatgtc
     1141 atgttgcttt ttaacattta ctatgtagtt ttgataaaat gatttgaata acagtgatgg
     1201 acaattcttt tttattttta acattatttt gataataatg attatttatt taaaaataca
     1261 ttagataaca tttgttacga cacgaaaaag cttcacaaac aaatatcgtt attttaaaat
     1321 gagggcgaac tcacacaatt aggaggaata gtatgccatt tattgtttac ttttttcttt
     1381 cattactcgt gatctatatt ccattaccaa catttttcct gctcgtcaat cgtctagcag
     1441 agcaacatga tacgattgct gctcttacga aactttgttt acttctattt gtttgtctag
     1501 attactttac gagctattat tggctaaaac agacaagagt aaaaaagaga tattatttct
     1561 tccttgtctt tttcaatctc tttgagtttc tgatccattg ctttttactt catcagtatc
     1621 atacgatcca tttgtggatc atctgggttt tcaaagtggt cgtattattt tgtatgttca
     1681 cctttcctat ctcaagaaaa tttagggagg cattgtttta ttaaagaagc ttctctgtat
     1741 caataaagac acatttaagg aaaaactttt aaaaatctaa ttgggaattt gcccgcataa
     1801 ataagaggct gtgacaaaag tcttgtcaca gcctcttatt ccgtataaac agtgttcaaa
     1861 agcttgcatc ttcgataata tgcccaacca aatgaaaaat atgagaagct atttttgttt
     1921 ggctgttctt attacttgat gaagaacagc tttttcacga cctcttttta aaagtctttt
     1981 tacagttttc gagcaaagaa tgatacgatt agtactaaaa tgatggcacc aatcaatgat
     2041 ggcacgattg ccattcctgc aatacttggt ccccaagaac ctaataatgc ttgaccaagt
     2101 gctgatccaa ctaaacctgc tacgacattt gcaataatcc ccatcgaaga acctttattc
     2161 gtgattgctc ctgcaattgc tccgataatt cctccgacga taagtgacca aataaatccc
     2221 atgtttcgtt cctcctaatg ttttacttcc ttattactga acccgtggtt ctgtattttc
     2281 ttttactttt tgtgcgccgc ttccagctgc tgattttact ttatctgttt gattcgaagc
     2341 aaattcaccg gtttttttcg ctgcgtcagt tactttgtct tgcaccgttt cttgatcttt
     2401 ttcaaattct tcttttgttt tgatgtccac tacgttagcg ttgacttcga tcacttctaa
     2461 atgtgtcata ttttttactt ctttgccgat tacttcttgc atttcttcaa agattttttc
     2521 gatatctttg ccatattcca caatgacatc catatcaact gctacttgtt ttttaccgac
     2581 ttctgtttcg atcccagccg ttacattatc tgtattcaca agttttcctg ccacattaga
     2641 gaagaaacca ccgtttgcag ataataaacc gtctacttgt tcaattgcga ttccgatgat
     2701 tttttgcaca actttatctt cataattcag ctcaccgcgc attgaatctt cctgttcaga
     2761 atcatgcttt ccttggttat ttgtatgatc agaaccaagt tttccttgtc cgtttgtgtg
     2821 gtcagaacta agtttttctt gtgctttcgc atggtcagaa ggatgttttt gctcttccac
     2881 aggtttagaa ggatcgattg catcatgttg ttctggaact ttagattctg gagttcctgg
     2941 atgatgaggt gcatgctttt ctgcatcatg tttcttctct tctactcctt gttgaacgtt
     3001 agggactact ggatctggat gtgcagatac tggtgctgtt ttgttctttt catcgttcac
     3061 accatttgat tggtttggtg tcgttgggtc tggatgtgca gacactggtt tttggttgtt
     3121 cattttattg ttattatttt tgttattgtt attcatggat gtttcctcct aagatttaaa
     3181 atagtgatct aagattttcg tttgtttcag ataaaaaccg atagcgacgc cgatcgctgc
     3241 catgatcaag atcaataagg tcttgaaaaa gcccacggtg atccataaaa cggctaatat
     3301 caatccaagt accccaaaga tgatagggag cttgtatgtc gaaaatagtt ctttcatttt
     3361 tggtcccccc ctattctact cgtttgcggt cattgcttcg tttagctggt tcttcaaagc
     3421 ctgtgtattt caccgatact ttcactggct cttttgttcc caatatgtgt tgtacttcat
     3481 tttcgatttc tttcatcaat gttcctgttt taccaatgag tgctgaagtt cgtttcaact
     3541 ccccttttac attgacggcg atcttgtttt tcgttgcttt gacatttact tttggtgaat
     3601 ggatgaattc atcttcatgc aagtgagaac gaaccattcc ttcaattgct tttttatcca
     3661 acgtcaactc cccatcacca tgtttcagta gaaagtttcc gattgttttt ggataaaaca
     3721 ggaaaacaag taacgtgata attgtcaaaa tagcaaatgc aacggcaagc caaaatacat
     3781 attgttgcac gtatgaatta gtgatggaaa agaatcgaaa cttctctagc tgaaaaggca
     3841 atggataggt tgcacttaat agtatcgcaa acaaaagcac agatagcaaa agtaatgttc
     3901 cgatcactcc gatcgttttt acacctttat tcattttcgc acctcctcat aggggtgaaa
     3961 cgatcaactt cttataagtt gatcgcttct ccaccagtta ctccgtaaat ttgggctgta
     4021 acataacttg ctttattcga tgctaagaaa acataaactg gtgctaattc tcctggctga
     4081 cctgcacgtt ctagtaaact ttgctgacca aattcaggga tcgaatcaga aggttgtcct
     4141 tcgtctagtt gtaatggtgt ccagattgga ccaggtgcta caccatttac acggattcct
     4201 ttaggtgcaa gctgttttgc taaaccgatc gtaaagttag cgattgaagc ttttgttgct
     4261 gcgtaatcta ataaatgttc gcttggattg aatgcttgaa cagaagttgt tgttacgatt
     4321 gctcctcctg ctgggatatg cggaacagct tctttcacta aagcaaacat agcgatgatg
     4381 tttatcataa atgtgtcttt gacttgctca agtggtaact cagcaataga ctgacaagaa
     4441 atttgctgtc ctgcgtttag taccatcgta tctagcccgc caagttcact cacggcttta
     4501 tctatgattt ctttaggtgc ttgttcgtca cgaagatcaa aaggtagtaa gacaatctta
     4561 cgtcctgcat ctttcacgta ttttgctact tcctctgcat ctttttcttc tcctgggaag
     4621 aattgaagag cgatatctgc cccttctcta gcaaatgcaa tcgctgctgc acgaccgatc
     4681 cctgaatctc caccagtgat tagtacacgg cggttttcta gttgattatt gccttcatag
     4741 gattcttctc cacaatcagg aacaggaatc atattctgct gtaaagctgg tgtatcctga
     4801 tcttgtttag gaaatttctc cgtatggtac aagtttcgcg ggtcgattaa ttttggttct
     4861 gtcattgctg tattcctcct agtttttaaa ttgtattgcc aaaacaaatt tctttgtttg
     4921 atacctgttt taaatagatt ctttaatttg tataatcttt ccggtataaa tggtaaccta
     4981 aaactactgc tgctatcaaa gagccaaaga ctacgagtat aaagcctaga gcagaacttt
     5041 tacccggtaa tcctcctgta ttcattcccc aaatccctgc gaacaacgcc ggaacggaaa
     5101 tgattaatgc agctgaatct agatatttca tgatatgatt tagattattg tccatcatcc
     5161 cgttgaatag atcaccaata ctctctaata aatctcgata gatctcgatc attttttctg
     5221 tttgtcgctg tctcaaatgg acatcataga gtagctgttc atcgtctaaa cgtttgatga
     5281 aagtttcaga attccataat tgatccattg tttcccgttg atctcttaag gtatgatcga
     5341 tatatacgat gtctctttca agatctgcct gtttatacag ttcttcattt tccgttgttt
     5401 tacgagctgc tttatccaaa gcgtcaattc gctttttcat ctctcttagt tctttcacgt
     5461 aatgggtgta aatatctaat aatgtatacg tgatgatctt ttcaaacgag tttaaatgat
     5521 ttccatgctt tgtcagcagc cgatccacaa attcggtttt tctatcgata caagtaatca
     5581 agagattatc ggaaagtaca aaagatattg gcgttaattt tttttcgata cgattatctg
     5641 actgatctag attaatcact accaaagaaa atggatgctc tagtttcgtc tcataaaggc
     5701 gctccatccg agaaacttct tccggatatt cggttcctac gaaaatatca tcaggaagct
     5761 cgaattcttc gattgcttgc tcaatctctt cgggctttgc actatcaagt acgagccaat
     5821 tatatttttt ttcatctgtt tctattacat tcttttcttc atccacaaag tagtatctca
     5881 atgtatacct cctttttgga ctttctcgaa actattagct gaagaagcag ttctttaatt
     5941 tgatattata atcgagtact gcgatttcat tttctaatga aaagctgtca agctgtttgt
     6001 tgaagtccat gttcaatcgt ttaccgaaat aattttgctc taatttagat gtatagatgt
     6061 ccaataatgg atgctcgatt ttagtcgcat attctccaaa ttccgaaaca aaaggaatga
     6121 tttgaaaaaa gctgcctttt gcaaaagcac ctttgtccct tagaaatgga tcggaatcat
     6181 tgataagcgc gagtgcgatt tttgtatttt ccccaacctt ttcaattaac gtctctactg
     6241 catttttaga cattgtaatt tccattccta ctccccctct tcagttgttg tcttctatta
     6301 tttaacaact acatctgttt ttggttgaac ttttgcatat tttggatatt tagggaattt
     6361 agctacacaa cggaaagccg tcatccctgc atatttttcg taatatttct tcgttggccc
     6421 ttcaaaatcc ttgctccacg gtttcacatg tcctgaaaaa tggataatgt aaggattgtt
     6481 gcgagtttct tcgtattcac gttcacctgc tgctgttgga tgtttttttg cttttgccat
     6541 gatatagcct tgtgcattcc aacgcggatg aagtggtaac caacggtcat gtaagatagc
     6601 atttaatgca tcttggtcat ggaatcgtaa tttttctgga ttatgttcga tgaaatctaa
     6661 tactttttgc gtaatatttt catctaacca ttttttcaca ttgatcagca tcaaacctga
     6721 gttgaaataa cgcattgatt tagctgaaat tttcattttt tccagtcgtt gatggaaccc
     6781 agcgtcttca acagctgcta cgattgaatc accgaagtca agacgccata atttagagat
     6841 atcttgtaaa gcgatcatgt cgcaatccat atataagata cgttcaactt ctgtgccgcg
     6901 gaacaattcc ggaatcgcaa tacgataata agctgtttct ggaatacgat cgctgattac
     6961 cacatttgta aagaattctt tattgatttt caagaattcc acatctgagt tcattcgtgc
     7021 gtttttcact gagaagcgta aaagctgttt actttcaaaa tcgatatcat cgtcaatcac
     7081 atagaaccga acaaatttag acgggttgca attatctaat actgagacaa ataatgcagc
     7141 taaatgtggt acgaattttg tgttacaact tgcgactact gctatttctt ttttattcat
     7201 tgatctcgcc tactttcgtt agagtactgt gtgctaattt ttttaagtat aaattggtat
     7261 acggatgatc tttcaacgta ttccaaggtt tatcatgtcc tgtaaagtga acgatcgaag
     7321 gtttttcatt tccttcttta tactgacgtt catatttttc atttggtgct ggatgacgtt
     7381 caaaaatcag cgaagtctgc atgttccatt ttggatgaag ttgttcccaa tcttcataaa
     7441 gtacagcatt caaagcatct tggtcgtgat agatgattcg atcgccattt tcactgaggt
     7501 aatggatagt tttttctgta atttcttttt tattccattg atcaatgtca atcaccatca
     7561 ctcctgaatt gaagtacaag tcatcggaat cgattcctaa tctttccaaa gctttcactt
     7621 ggcctggatc gataatagcg ccgattgttt ttccatttaa cggctcatca tatagtttta
     7681 cgatatcgtc tagtactaac acatctgaat ctaagtaaag gactttctta taatcctctt
     7741 tagctaataa gtttggtaaa gaaattctta agtaagcggt agttgtgata tggtcactga
     7801 ctaggaagtc ttcgtagata tctttttcca ctgtcaggaa ttggatactt gcattacttg
     7861 aatatttatt taccgtttct tccaatcctt ctttactata ttctgataga ccatcgtcaa
     7921 tcacataaaa ttttatgcgt cttgttttat tgcaattttc taatgcagtt gcgatcatca
     7981 cgctcaagta aggagcataa ttttcgtctg aagcagttac cacaggaact acgccgtatc
     8041 tcttctccat caaaatcact cctttttcta atctttttct aacctgttct acatctagaa
     8101 tattcctcat ttggttttaa agcaaaatat atgctttgtt ttcattgaat attttaaaat
     8161 ttttttatca caaaacgcat cggtaagcgc taactattcg tttttcatgg ctttttattc
     8221 aaaggt
//
LOCUS       DAZKCY010000108         8172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000108 DAZKCY010000000
VERSION     DAZKCY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.109"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..617
                     /locus_tag="ACG8XC_002250"
     CDS             <1..617
                     /locus_tag="ACG8XC_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9191923.1"
                     /translation="LQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVL
                     VREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQ
                     TKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQS
                     KYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
     gene            complement(777..1682)
                     /gene="xerC"
                     /locus_tag="ACG8XC_002251"
     CDS             complement(777..1682)
                     /gene="xerC"
                     /locus_tag="ACG8XC_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP9191924.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(1880..3256)
                     /gene="trmFO"
                     /locus_tag="ACG8XC_002252"
     CDS             complement(1880..3256)
                     /gene="trmFO"
                     /locus_tag="ACG8XC_002252"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFP9191925.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(3225..5303)
                     /gene="topA"
                     /locus_tag="ACG8XC_002253"
     CDS             complement(3225..5303)
                     /gene="topA"
                     /locus_tag="ACG8XC_002253"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP9191926.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHGHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(5425..6282)
                     /gene="dprA"
                     /locus_tag="ACG8XC_002254"
     CDS             complement(5425..6282)
                     /gene="dprA"
                     /locus_tag="ACG8XC_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFP9191927.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(6338..7105)
                     /locus_tag="ACG8XC_002255"
     CDS             complement(6338..7105)
                     /locus_tag="ACG8XC_002255"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP9191928.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(7098..7958)
                     /gene="ylqF"
                     /locus_tag="ACG8XC_002256"
     CDS             complement(7098..7958)
                     /gene="ylqF"
                     /locus_tag="ACG8XC_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFP9191929.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            complement(8052..>8172)
                     /locus_tag="ACG8XC_002257"
     CDS             complement(8052..>8172)
                     /locus_tag="ACG8XC_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9191930.1"
                     /translation="FIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         2208 a         1781 c         1427 g         2756 t
ORIGIN      
        1 tacttcaaga aaaaggaaaa ggccgtttca agcaaccaga aaaaatcgct aaagcaattc
       61 aacgtgcaat taccatgagt taccgcctag gtgctcttgc acaagaatca attaatgttg
      121 ttttaagtgt tctcgtacga gaaatacgag cacttgaaaa aaatatcaaa gaattagata
      181 aagccatcga acaagtaatt gttgtcattc cagaatatca atgcttaacc agtattccag
      241 gcgttggtaa agtctatgct gccggtttaa tcgctgaaat tggtcaaatc gaaagatttg
      301 aagatcagac aaaattggca aaatacgctg gattaagttg gaaagtgaac caatctggaa
      361 actatcagtc gcaaaataca cctctgacaa aacaaggaaa tcgatatttt agatactact
      421 tagttgaagc tgccaactct gtaaaaaatt atcttcctga atacaaagct ttttatcaaa
      481 gtaaatataa ggaggttcca aagcatcaac acaaacgtgc actcgtccta accgcaagaa
      541 aatttacgcg tctggtggat acgctactac gtaaacacca actctatacg ccaccaagga
      601 gtgtgataga gaaataacaa tccgttattg acgcaaatcc ttgaatcaac ccgaaaaaaa
      661 tttgattttg atcgggtcta gtttcgtgcg cttcaaaaca tattactcaa taaagaaaat
      721 cttaaatttc atcttgactt atcaccatta gactaaattt gttcttttcc ttatttttac
      781 gctcttggat ggaaagaacg atagtttttt tgtaaacttt cttttgttac atgggcatag
      841 atctgggtag tcgataaatt ggcatgtcct agcaattctt ggactgtccg caaatctgcc
      901 ccattattta ataaatgagt agcaaatgta tggcgcagca tgtgaggatg gatatcacta
      961 tccaaactgc tttttttgat catttgattc aagacatatt caattccagt aggcgtgatt
     1021 ggttttccat gatgattcac aaaaacgata tcatgctctt cgtgatattt tgtcatcaat
     1081 acctggcggc cttccctgaa atattcttgc agagcatctt gagcaaaaga acctagagga
     1141 gcatatctct ctttgttccc tttaccatgg atcaacatga cctctgaatc aaaatcaatg
     1201 tctgataccg acaaactgct acactcactt aaacggatgc ctgacccgta aaggacttcc
     1261 agtaaagcgc ggttcctcaa atccaaagga ctatctcctg ctacgctgtc aaaaagtgct
     1321 tgcatttcat tttcataaaa aaaacgtggc agtctcaaat ttttcttttt gagatgaacg
     1381 taagaaaatg gattttcttt tatcacttct tctttgagaa gatactgata aaacgaacgt
     1441 aggctggcta tcttgcgact gatagaattt ctgctatagc taggacgatg atcagctaat
     1501 gcactcaaat agacacgaac cgtaaaatat tccgccttca gcagatcagc gtctccagat
     1561 tcctccaaaa aatgtttgaa atggagaatg tcttcttcat atgcttcttt cgttttttct
     1621 gaatatcctc tttcgacaat caaataacgc aaaaattttt gttcccacgt ggcttctgtc
     1681 attttctcac cttccttttg tcccagagaa taatttagca cagcgtttat aaaaagacaa
     1741 ataaattgtc agaatttaac caatatttct aagtttgttc acaattggcg caattcctca
     1801 cattttgttt gagaaattct taagaaaaac gcaagaaaaa gagcgagact atccgtctca
     1861 ccctcttcat ggagtttcct tactcatttg ggattgcagt tttttctaat tctgttttta
     1921 ttgcctcatt agcttttaaa gctcgttgtg caattgcttc atatcttgct tttttgtctc
     1981 gaatgcgctc tggcagttca ggaaacagac catagttggc attcatcggt tggaaatgtt
     2041 ttccttccgc atgagtaatg tagtaagcca tacttccaat agctgtttct tgtggaaact
     2101 cgactggttc catttctttt gctaagcgtg cggcgttgat tccagcaagt agtccgctcg
     2161 ctgcactttc aacatagcct tctacaccag tcatctgacc tgcaaagaac agatcttcgc
     2221 gtttcgttga ttggtaagtc ggtttcagca attctggcga attcataaag ctgtttcggt
     2281 gcatcacacc atagcgaacg aattcagcat tttctaaccc aggaatcatc cggaaaacac
     2341 gtttttgctc gccccatttc aagtgagtct gaaagcccac gatgttgtat aaagaagctg
     2401 cagcattgtc ttgacgcagc tggataacag cataaggacg tttgccagtt ttaggatctt
     2461 ccaatccaac tggtttcata ggtccgaaaa gcatcgtttt gaatcctcgc tgtgccatta
     2521 cttcaattgg catacatcct tcaaaatatt tttctttctc aaaagattta agttctgcca
     2581 cttcggcatt gattagtgcc tcatggaaag ctttgaattc ctcttctgtc atcggacagt
     2641 tcaagtaagc tgcttctcct ttgtcatagc gggacttcaa atatacttta tccatatcga
     2701 tcgtagcttt gtctacgatt ggtgcagctg catcatagaa ataaaaacca tctgaaccat
     2761 tgaatgcttt gatgctttct gctaatggat gagaagtcaa aggtcctgtt gctactacgg
     2821 tgattccttc cggtatttca gtaatttctt cgtttatcac tgttactaat ggatgctctt
     2881 tgacttttcg ggtaattgtt tcagaaaaag aatcgcggtc aacagctaag gcaccaccag
     2941 ctggcactgc tgtttcatct gccgaagtga ttactaccga attcaatcgg cgcatttctt
     3001 ctttcaataa ccctactgca ttagccaaac tgtttcctct taatgaattg gaacatacta
     3061 gttcagcaaa attacttgtg tgatgcgcct cagtcgattt gactggacgc atttcatata
     3121 aacggacagg gactcctgct tgtgcgatct gccaagcagc ttcacttccg gcaagccctg
     3181 ctccaacaac atttaccatt ttcaatgaaa gaacctcttt tctattattt ttggatgtct
     3241 tcttcgtaat ctccatttac acagactact tgtttaccgc ctttgacttt cttctcgacc
     3301 agatatccgt cacattttgg acaatttctg cccactggtt tatcccaaga agtgaattca
     3361 cattctggga aacgggaaca accatagaat agtctgtttt tcttagattt tcgttcaatg
     3421 acttgtcctt catgacatac tgggcaagtc acaccgattt cttttacgat tggtttcgta
     3481 ttccgacaat caggaaaatt actgcaagca taaaacttcc catatttccc taattttatc
     3541 accatcggat gtccacagac atcacaatcg aatcctgctg gttcatcttt gatttgaatt
     3601 ttttcgattt tttcttccgc attcgtcagt tctttttcaa acggtttata gaaacgatca
     3661 accactttga cccacgcttc cgtgccttct tcaattttat ccaaatctcc ttccatttct
     3721 gctgtgaaat ggatatcgac gatttgaggg aagaattcac agattaatga attgacaatt
     3781 tctcctagtt cagtcggctc aaatcttttt tgtgtcaatt ttacatagta gcgtctttgg
     3841 atcgtatcaa gtgttggtgc ataagtggaa ggtcgcccta ccccgttttc ttctagtgca
     3901 cgaatcaacg tagcctcact aaaacgtgct ggtggttgag taaaatgttg ttttggttca
     3961 atatcaacag attggactac atcgccttct tcaagttctg gaagaatatt ttctttctct
     4021 tctttgccgt catctctacc ttccacatac acttgcatga atcctttgaa tttgattttt
     4081 gaaccgttcg caatgaagat gacaccattt tgttctaatg tcactttcat tgtatctaaa
     4141 atagctggtg tcatctgact agccacaaaa cgtgaccaga tcaatgtata aagtttcaat
     4201 tgatctttat ctaaatattg tttgagttca tctggtgtac gaagcacact cgttggacgc
     4261 acagcttcat gggcatcttg tgcgccttga gcatttttga tctttcggtg accatgtcta
     4321 gaaaattctt ttccatacgt atcttcaata aatgctgcgg cctctgcttt tgctgtatcg
     4381 gcaatccggg tagaatcagt acgcatgtat gtaatcaagc cgactgttcc ttgtttaccc
     4441 aaagtgattc cttcatataa ttgctgtgct accatcatgg tttttcttgt tcgaaagttt
     4501 aacttacggg cagcttcttg ttgcaggcta cttgtagtga aaggattcgc agggtttctt
     4561 ttgcgctctt tcttttctac ttttttaact tcgaagtctg ttccatcaat tctagcagtg
     4621 acttctttta cactttgtgc atctggtaat ttctttttct taccgtcgat tccccagaaa
     4681 tttgctttga atttcttgcg agctttttta aaattaccgt cgatgctcca atattcttct
     4741 ggaacaaatt ctcggatctc tttttcacgg tcaataataa tttttaaagc gacagattgg
     4801 acacgtcctg cacttaaccc tttcttgacc ttacgccaga gaataggact gatcgaataa
     4861 ccaacgattc tgtctaaggt acgacgagct tgctgtgcat ctactaaatc tacatcgatc
     4921 gttcttggtt ccttaaaagc tgctttgact gcgtcttttg tgatttcatt gaagaccacc
     4981 cgattttttt ctttcagatc caacccaagt aggtacgcta aatgccaagc aatcgcttct
     5041 ccttctctat ccgggtcact tgccaagtaa actttttgag ctttttttgc tgcggctttc
     5101 aggcttttga tgacatcgcc ttttccgcgt atagaaatat aatggggttc ataattgttt
     5161 tcaaaatcga tacccatttt acttttcggt aaatctctta tatggccaac actggccatt
     5221 actttatagt ttcgtccaag atatttctca attgtcttgg cttttgcagg tgattctaca
     5281 atcactaaat atttatacgc cattactgaa tggactcctt tccattgtcg tgttcctact
     5341 attaaaagag gattcataca aatcgtagct aatcatatcc attcagaaaa cgtttgtcaa
     5401 gaaaagcaaa tgaaaagtat ggtgttattc tttaaattct gccaagatat cttcacccga
     5461 aaccgtgcaa actgcaccgt cttggatcaa atgatgacat ccggtagaat gttctgttaa
     5521 aatatttcct ggaacagaaa aaactgttct tccaagatcc aatgcttgct gtgctgttat
     5581 cagagagccg ctttttttct tggcttcaaa gacacatgtt cctttagaca aagctgcgat
     5641 aatccgattt ctcattggaa aacaatgctt tttcgggccg gattgaggag gatattcact
     5701 caaaagaaga tgatcatttg caatcacctc ttgcaagtca cgattttcag caggatagac
     5761 ttgctctatc cctgttccaa tgactgcgat tgtccgtcca ccatgagaga ttgctgcttg
     5821 gtgaccgtat gtatctatcc ctttcgcgca gccactgacg atcacatatc cagcttctat
     5881 cactggtttt agtaaataat aaactgtttt tttggctaaa gcagaagtgt gtcttccacc
     5941 aacaacgctt aacatgttac aagaaagtaa agatagatca ccattataaa acagtacaat
     6001 tggcggatag gtcaagtgaa gcagctgagg gggatatttg ggatcaaagc aagtgataaa
     6061 cgactgtttc gattggattt tgccccagtt agcgtgaatt tcctgccaac ttttgcgaaa
     6121 cagctcccgg tgtttggtta ttttacctat cttaatgatg tcttcaaatc cgatttcttt
     6181 ataaggatac tgcatcatcc agttagcaag tgcccatttt ccttgactac tgattccttt
     6241 tgtataagca agtttcaata aattttcttc tatttgatac atcataaaac cgcctttcgt
     6301 ctctctttct aataagatac gtaaaagcgg ctgtttttta ttgtaaatat ttttttacag
     6361 gtgcaaaact tttacgatgg attggcgtaa ttccttgtcg ttcaagtcct tctaagtgtt
     6421 cctttgttcc ataacctgca ttatgcgaga aaccatatcc aggataaagc acgtcatatt
     6481 ctttcatcaa ttggtctcga tatactttag caatgatact tgcagcagca atcgacacag
     6541 atcgtgcatc ccctttgatt attttttcct gctgaatagg caaatccaag gtcatcgcat
     6601 cgatcaacaa atagcttggt acaatggaca atttttctac tgcttggatc atcgctaatt
     6661 tacttgcttc ataaatattc acatcatcga ttgtctgctg atcaacgatc ccgatcccga
     6721 ttgccagggc tttttcttga acttgttgat aaagaagttc tcgctttttc tctgataatt
     6781 gtttggagtc attcagccct aatatgatct catttttcgg caagataact gcagctgcca
     6841 cgacaggtcc agctaatggt cctcttccta cttcatcaat gccggcaatc cattggtgac
     6901 cttgtgcgta cccttgcttt tcaaagcaaa acatttcttc atatttttcc ttaattaatg
     6961 cttccttttt caatcgtcgt tcaaaagaag ccagtgcttg ctgcacgcct ttgcgtggat
     7021 catttcttaa tgattctagt gtaggatcat ctattctctg tatgcttttc aatcgttctt
     7081 tgatactttg gatggattca gtcatcactt ttttgctcca tttcttcgca acgatccagt
     7141 gtgtatcgtc caagttttcc ttgacgaata tcatgcacga ctaattcgct gccacgatca
     7201 tagtcttctt tatacccacg ttttgctgta atatccatca gtaattccgg tgccaatttt
     7261 tcttcttgct cgattgataa accataacga tcgctgattc ttcctgaata atatcgggca
     7321 aaaaagtcta atccgtaaat cgctaaatca tctaaatgga caagctggtc tttgatcgcg
     7381 cctgtcagcg ctagtttttt gccgatttct tcatcttcaa atttgggcca taaaatccct
     7441 ggtgtgtcta acaattccaa ctcattacct aaacgaagcc actgctgacc ttttgttacg
     7501 ccaggcttat ttcctgtctg tgcaattttc ttgcctacta gtcgattcat caacgtagat
     7561 ttcccaacat ttggaatacc taagaccatt gcccgaatag cgcgaggctt gatcccccga
     7621 gcagcatctc ttgcccgttt ttctttcaat gcttctttag ccgcaggtac tagaccttta
     7681 attcctttac tttcttgtgc cgtgatagct aatgcaagat ggccttgttt acgaaaatat
     7741 tcttgccaca gctttgtctg ttgtggatct gccagatccg ctttattcaa taaaacaagg
     7801 cgaggttttt gctgaatgat ctgatctagc attggattac gagaagataa aggtaatcgt
     7861 gcgtctacca attcaaatac gatgtccaca tatttcattt tttcagaaac ttctcgtcgg
     7921 gcttttgcca tatgtcccgg aaaccattgg atggtcattg ttctctccat tcctttcata
     7981 ccggaatatt ccccggcttt acttgttttt ttctttttca atataaattc acttttttat
     8041 tatcttaatt gtcaaattaa gttagacaaa tcatcaatac ttacgtaact caaaaatact
     8101 tccaaaggtg ttcgataatt cagtgatttt ctaggaatat tatttcgttt cgatgcgaca
     8161 gattgaataa aa
//
LOCUS       DAZKCY010000109         8167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000109 DAZKCY010000000
VERSION     DAZKCY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.110"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8XC_002258"
     CDS             476..1339
                     /locus_tag="ACG8XC_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9191931.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8XC_002259"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8XC_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9191932.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8XC_002260"
     CDS             3339..3860
                     /locus_tag="ACG8XC_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP9191933.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8XC_002261"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8XC_002261"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFP9191934.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8XC_002262"
     CDS             5941..6759
                     /locus_tag="ACG8XC_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFP9191935.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8XC_002263"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8XC_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191936.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8XC_002264"
     CDS             7228..7806
                     /locus_tag="ACG8XC_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP9191937.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8XC_002265"
     CDS             7901..8038
                     /locus_tag="ACG8XC_002265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191938.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2444 a         1474 c         1612 g         2637 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgtg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatag
//
LOCUS       DAZKCY010000110         8137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000110 DAZKCY010000000
VERSION     DAZKCY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.111"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            169..1908
                     /gene="yfcC"
                     /locus_tag="ACG8XC_002266"
     CDS             169..1908
                     /gene="yfcC"
                     /locus_tag="ACG8XC_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP9191939.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2065..3249
                     /gene="iadA"
                     /locus_tag="ACG8XC_002267"
     CDS             2065..3249
                     /gene="iadA"
                     /locus_tag="ACG8XC_002267"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9191940.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3360..3563
                     /locus_tag="ACG8XC_002268"
     CDS             3360..3563
                     /locus_tag="ACG8XC_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9191941.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3664..4326)
                     /locus_tag="ACG8XC_002269"
     CDS             complement(3664..4326)
                     /locus_tag="ACG8XC_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9191942.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4339..5841)
                     /locus_tag="ACG8XC_002270"
     CDS             complement(4339..5841)
                     /locus_tag="ACG8XC_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9191943.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6389..8068
                     /locus_tag="ACG8XC_002271"
     CDS             6389..8068
                     /locus_tag="ACG8XC_002271"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP9191944.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
BASE COUNT         2538 a         1440 c         1752 g         2407 t
ORIGIN      
        1 aatggaaaga gaaaaaaata atcatacata ggagaaaaaa aacaaaatag aaaaaagtga
       61 ttttctaata aattattcat gtggaaatgt catttttact gtataattca tttgattatc
      121 cattttttat tcatcaaaat tactgaaaaa agaaaggaag aaataaattt gaagaaaaca
      181 aattcttcag gaaaaccgaa acgcgacttc actaaattat cgacacccca tacgtatgtc
      241 attatttttg gtgtagtcat ctttgcatgg atactgactt ttgtcgttcc agcaggaaag
      301 ttcagcacgc aagatattga atacaaagat gcaaatggcg aaacaagtac tcgtactgtt
      361 ttacgtcaag attccttccg ttatgcttac gagctggata agtcgtatgt gtttgatcaa
      421 ttagaagaac tgcaagatca tccagcagaa cgtgaaaaac tggatgttcc ggaaaaagga
      481 ttggaaaaag tcattgctga cggagaaaaa aatcttacgc aggaaaaact agatgaaatc
      541 tccttgacag atgatgtgct ttacgatcag tacggcgaaa atatctatga tacttctaaa
      601 aaattgcata aaacagccaa aatatgggga acagatgatt ttggcggctt tggtttctta
      661 aattttgttt ttgaaggact agtatctgga gataagtatg gatctgctgt agggatcgct
      721 gcattgatcc ttgttgttgg aggtgctttt ggtattatta tgcgtaccgg agcgatcgac
      781 gcaggtatct atgcgtttat cagtaaaact aagggactgg agcgtttagc tttaccgcta
      841 ttattctttg ctttttcatt cggtggcgct acattcggga tggcagaaga ggtaattcca
      901 ttctcaatgg tgatggttcc atttgtcatt gcgttaggat atgactcgat cgtagcagta
      961 accgtgactt atgtcgcatc tcaagtcgga aatgcgacct cttggatgag cccgtttagc
     1021 gtagcggtgg ctcaagggat tgctggaatc cccgtgttgt ctggtgcgac attccgattg
     1081 attatgtggg tagttgttac tgcattagcc gctggttata tgatgatata tgcagaaaaa
     1141 atccgtaaaa aaccagaaag ttcgttgact tacaaatcag atgattattt ccgttctcat
     1201 atcaaaaaga catctgatga aaataaaaaa ttcatgcttg ggcataaatt gatcttatta
     1261 gagatgttag ttgtccttgt ctggatcgtt tggggcgtga ctcaaaaagg ctattacatt
     1321 ccagaaatcg cttctcaatt ctttgtgatg ggattagttg ctggaatcat tgctgttctt
     1381 ttcaaattag atggaatgaa agtcaatgac atcgcttctt ctttccaatc tggtgcagct
     1441 gatttagctg gtacagcaat cgtagtaggt atggccaaag ggattcttct tgtcttggga
     1501 ggatctgatg catctgttcc ttcagctttg aatacgattt tgcatgggat cgggacggcg
     1561 ttgaccggtg ttccagctgt cattggtgct tgggcgatgt acatcttcca aagtttgttc
     1621 aacttggttg ttacatcaaa ttcaggacaa gcagctttga ctatgccaat catggctcca
     1681 ctggcagatt tagtaggtgt atccagacaa gtagcagttc ttgcttatca gcttggttca
     1741 ggttttatgg acgcttttac acccgtatca gccagcttga ttggtgtgct aggtgttgca
     1801 cggatcgaat gggcaaaatg ggcaagattc caaatcaaga tgcaaggatt cctgtttgtt
     1861 ttaggaacta ttttcatcat gattgccatt gcaataggtc tgcagtagta ctggagacct
     1921 gtaaataaaa aagtaatcca aacagtagat aaggactcca agaaccgtca ttttacggta
     1981 aaatcaaagg agtaaagatc ctatcgtttg gattattttt taagatccta tcatcagtta
     2041 tacttaaaga aagaggaaaa atgtatgaaa atcattcgac aaattgaagt gtttgcacca
     2101 gattacttag gaaaaatgga tgtactgatt gccggtggga agattttggc tgtagaagaa
     2161 cagctagaag gcggctacga aggagtggaa gtagaagaac tttcaggtga gggaaaagta
     2221 ctaaccccgg gtttcattga ctgtcatttc catattttag gtggaggagg agaaggcggt
     2281 tatcaaaatc gaacgccaga agtcacattg agccaattga ctacagctgg tgtaacgaca
     2341 gttgtcggtt gtttgggaac agacggagaa ggtagagata tgactgcgtt gatcagcaaa
     2401 gcgaaaggtc tggaagcaga aggaatctcc acttatgtat acgaaggttc gtaccgattg
     2461 ccggtaaaaa cagtaacaga ttctatcatc aaagactttt tgacaattga taaaattatc
     2521 ggaatcggag aaatcgcagt atccgatcac cgttcttctc agccaagttt tgaagagttc
     2581 gctcgtgcag tagcagatgc tcgtgtagga ggcatgttat ccggaaaagc tgggatcatc
     2641 aatgtccact taggcggagg aaaaagaaag ctagagctat tgacacgaat cgtagaagaa
     2701 acagaaattc cgatcacaca atttttgcct actcatgcaa atcgcacacc agaattattt
     2761 gaagcttgcg tggcatttgc caaacgtggt ggcacgattg acttcactgc tagcgaagat
     2821 cctgattttt gggagaagac agatggtgaa gttcgcttca gcaaagcact gaaacgattg
     2881 atcgaggaag atgtttcttt agataatttc acgatgacct cagatggaca aggaagcttg
     2941 ccttattttg atgaaaacaa tcatttccta gggttaggag taggaagtgc caaggcattg
     3001 ctagtaggga tcaaagaagc tgtgcaaaaa gaaagtatcc ctttggaaat cgccttacga
     3061 gctataacca gtaatccagc acgtatattg aaattggata aaaaaggaaa aatcgaaatt
     3121 ggtgctgatg cagatctgtg tatcttggat aaagaaacgc ttgatattga tacagttatc
     3181 gctaaaggag aaataatggt ccaagaaaaa gaagtcaaag tatgggggac tttcgaaaaa
     3241 agtttttaat caacagctct aactatcttt tttagaaaac gagcgttctg tttgtctgta
     3301 tctgttgttg atgttaccct gaaggtagat caataaacaa actaagagga ggaagcacaa
     3361 tggcagattt aaaaggacgt tctaaagata tgaaagataa agtagtcggt gaaggaaaag
     3421 aagcatacgg caaagcaaca gacgacaaga gcaaagaaac agaagggaaa gcacaatcac
     3481 taggtgctga catcaaagaa aaagctcgaa atttaggcga cgaagtcaaa gacaagtttt
     3541 ctcgaaaaga cgaaaaaaaa taaaaaccac ttattactaa ttgaatcaag caaataaaaa
     3601 atccgaacca tatctgcgag acagatatgg ttcggatttt ttatttgatc attatcctgt
     3661 tttttaaact tcgatttttt gaaatttttt attttttagt tcaaagactt catcacagct
     3721 ttttgctact tcaatctcat gagtcacgat gataatacag cgattttgtt catgggcgat
     3781 ttcttggaaa agtttgacga tatcttgtga agttgtttca tctagatttc cagtaggctc
     3841 atccgctacg attagttgat gttcacagca gatagcacga acgatagcga ctcgctgctg
     3901 ctgacctccc gaaagtttgg tcacctgttt ttttgctaat atttcatcaa ttcctacacg
     3961 agccagtcga tctaaagcaa cttgtcgata attctcttgc ttggtcttag tgatttccat
     4021 tgctgctatg acgttgtcta gagccgataa ataaggaagc aagttgtaag cttggaaaat
     4081 aatcgagaca tcatttttac gataacttct taacccgatt ttgttcagtg aacgatcttg
     4141 atagtatacc tttccttctt ttggtgcatc tagtccagaa ataagcgaaa gaaaagtcgt
     4201 ttttccagaa ccacttgctc ctaaaatagc gtacattttt ccagcttcga aattcagttc
     4261 tacatcttta taaagcgcat cttgcggatc ttgataccaa aatcccaatt gttctgtccg
     4321 taacatcaaa ttcctccttt agtttaatag gatttttctt gggtttaatc ttaatatccc
     4381 tatagaagct aaaaagacag aaaacagact gattccaaat gctactccgg ctaatagtcc
     4441 caattgtgca ggtttgacat tgatttccaa ttcgctgact tgatcagaaa cagcaaatgg
     4501 attattcgtg gttccttgtt gaccatcgcg ttgaccgcct tgttcttgtc tgttacctgg
     4561 catttgtcca ccgccaggag cttgctggtt ttgtgtcgtg gtttctgtct gttggtcaag
     4621 tagctgctgt cccacggcat tcgcgacgac gtttccgcta agcgaagcaa ctcctaatgc
     4681 aagaatcata caaatagcca cttcggtaaa caattggaga atgattttac ttctggattc
     4741 tcctaaagat agcaagacgc ctaactcatg ccgacgctcc cgaatggtaa tcataatgat
     4801 cagagtaagt atgataatac cggctacagc cactagtaaa acaacatttt tagaaaaact
     4861 tgccacgttg tttaaaggtt ccaacattga ctgatacatg gaatcatttg attgtaagct
     4921 aaatgtatct gtgtcgatca gcttctcagc ttctttgaga aactcatcca ttttatctgg
     4981 atcagataga gtgtaaatag cagaatctat cgtgttatcg tcagatgtcc catttagttc
     5041 atttgcaaaa gtgtaagaag tgtaaagtgt attcgacgga ttcatgaagt tgaatttcat
     5101 acccatactg ccgcttgttt cgctggattc ataaattcct ttgatcgtca tttcataagt
     5161 ggtatcttca tcttcagtac tcgtgattgt gaaagtatct ccgactgcca ggtcatttgt
     5221 ttctgctaga gtagaatcaa tgactacatt gtttgtgcct ttgtcgctgt catctattcc
     5281 ttcaccatcc gtgatttttg ctgttccatc agaaaaatta ctattttgtg cagttgctga
     5341 cacaccactg atttgaaagt ctgcttgagt catttgcgga ttcccgccca ttcctccagc
     5401 aggttcaccg cctgcatcag tagtttctga actgtcttca cttgaatcag aactagtgat
     5461 ggcagttatt ccatcttttg ccatagctga ggcggaactt tcaaaggaat aactctttac
     5521 attatctaat tcagctattt tttccgcgtc cgttaaggaa actggtgtaa gagaaaaact
     5581 gcctggatct ggacgttctt cagtggtaga accgcttgaa gaagtttgtt cctgtttttt
     5641 aaatgaagct tctcgattgg cacttaaagt cactgttgct ccaacttctt tttgtgcttg
     5701 attcgctgct tcttctgcag cgcttcgaat cgtgaggcca gctagcacaa aaataagaat
     5761 ggctgtgaac acggcaaaca ataacaatga acgtccccat tttacttttg tacttttcca
     5821 tgcacgtttc ataaaattca tattttgcat ccctcctagt agatgcattt gataatagac
     5881 aacctatctt aaagctagct taaatcttat gctgtttgca caccatcaac atgaggttct
     5941 gtatatataa aaagtcacta tagttattgc aaaaaatgtg gtaaaatgaa aaaagtatgt
     6001 ttcaaaccga cgaagaagaa atgaaagagg aatagcaaaa gaaatcctag atgctacaga
     6061 gggattttta cgtgcatagt tgttctaaca attgctgata ctagcgattt agtttatttg
     6121 ttgtataata atggggaaca gctgaatact tatataagta ttctggctat agcgtttcgc
     6181 aaaagatcca ttttgttaat gaagtatatc caaaataaaa tgcccatcca actgtcagaa
     6241 cgcggatgtg ctggtaattt tcctgctgat tctccataag ttgagaaata gtgattttgc
     6301 atatgcttca ttggaatggc tcatatattt agataaagaa gaaaatcgac atctttaaac
     6361 ttggatgtca taagaagagg tgaagtttgt gagtaccatt aaaattatcc cattaggcgg
     6421 cgttcgtgaa aatggaaaaa acatgtatgt cgcagaagtg ggcgaggata tttttatttt
     6481 agattgtggg ctaaaatatc ctgaaaatga gttgttgggg atcgacgtcg taatccctga
     6541 ttttacgtat ttagaagaaa acattgatcg tgtagctggt gtctttttaa cacatggaca
     6601 tgcagatgcg atcggggcac ttccttactt gttgtcaaaa atttctgtac cagtattcgg
     6661 gacagagttg acgatcgagt tagcaaaatt gaatgtcagt cgtaatgaag cagccaaagg
     6721 atttaaggat ttccatgtgg tagatgagca tacagaaata gactttggtg aaacagtagt
     6781 gagctttttc cgtaccactc atacgatacc agattcagtg gggatcaatc tgaaaacaaa
     6841 agaaggtagt atcgtttata cgggagattt taaatttgac caaacggcta tcccaatgta
     6901 tcaaacagat tatgcacgat tggcggaaat cggcaaagaa ggagtcttag ccttactaag
     6961 tgaatcggca aatgcagaaa atcctcagcc tgtagcttct gaagcccaaa tagcagatga
     7021 agtatttgat acgatcaaat attgggaagg gcgtatcatc gtcgcttgtg tagcaagtaa
     7081 tttgcaacga gtgcaacaag tattggatgc agcagaaaaa gcacaacgaa aagttgtctt
     7141 gactggtcaa gatttcgaac gaattattcg cacagcgatg cgtttagaaa aacttcaatt
     7201 accaagtgaa gatctgcttg ttacgttgaa agacatgaaa aactatgatc cagaagaact
     7261 gatcatttta gaaactggcc gaatgggtga acccatcaag tctctgcaaa aaatggcaaa
     7321 cggtacacac cgtaatgtca agattgaaga aggagatttg gtttatatca ctactacgcc
     7381 aagcatcgcg atggaaacca tcgtggcgaa aacggaagat atcatttatc gagctggtgg
     7441 aacagtgaag ttgatctctg agaatatgcg cgtttctggc catgccaatc ctaatgactt
     7501 gcaattaatg atcaatttga tgaaaccaac atattttgtg ccagttcaag gagagtatcg
     7561 tgaattagct gctcatgcag atttggctca tgcagtaggg atgccataca aaaatatcta
     7621 tattactgga cgcggagacg tgctggaata taagaacaag cgtatgagtg ttgcaggaac
     7681 tacaagtgca gaaaatgtaa tgattgatgg attaggcata ggtgatatcg gaaacattgt
     7741 tttacgtgat cgaaaaatct tgtcagaaga cgggattttc gtagcggttg tcactatcag
     7801 tcggcgagaa aagaaaatca tttcaaaacc tcaaattact tcacggggat tcgtttacgt
     7861 aaaagctagc cgtgatctga tgaaagaaag cagcaatatg attgaaacaa tcgttgagaa
     7921 acatttagaa agcaatgatt ttgagtggag caaattaaag caagacatcc gagatcaatt
     7981 aggccgttac ttatttgaac aaacaaaacg ccgcccagtg atcttgccag tcattatgga
     8041 agctactcaa cgtcgaaata aaaaataaac aaaaagagta agatgaacct ctctatagag
     8101 tgctcgtctt gctctttttt tgcgaaaaac aaataat
//
LOCUS       DAZKCY010000111         8086 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000111 DAZKCY010000000
VERSION     DAZKCY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.112"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..2345)
                     /gene="helD"
                     /locus_tag="ACG8XC_002272"
     CDS             complement(66..2345)
                     /gene="helD"
                     /locus_tag="ACG8XC_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFP9191945.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            2623..4447
                     /locus_tag="ACG8XC_002273"
                     /pseudo
     CDS             2623..4447
                     /locus_tag="ACG8XC_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            4494..4673
                     /locus_tag="ACG8XC_002274"
     CDS             4494..4673
                     /locus_tag="ACG8XC_002274"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFP9191946.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            5010..6950
                     /gene="thrS"
                     /locus_tag="ACG8XC_002275"
     CDS             5010..6950
                     /gene="thrS"
                     /locus_tag="ACG8XC_002275"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP9191947.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            7174..8022
                     /locus_tag="ACG8XC_002276"
     CDS             7174..8022
                     /locus_tag="ACG8XC_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9191948.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
BASE COUNT         2541 a         1460 c         1713 g         2372 t
ORIGIN      
        1 tctgcaatta ttattgtaaa taaaaagaca actgctaaca ttaatgatag cagttgtctt
       61 tttacttaca gtactttttt ctcataagat gaaatatcca tattgtttaa caaaggactt
      121 tcattattgg aggtaaaaag ggtcaattca tgcatcgcac gtgtacacat ggtgtaaagg
      181 atcaatttgt cttgctgaga agaaaaatgt tctgcatcta catcccacaa gaaaactcgg
      241 tcaaactcca gaccttttgc taaaaaggct ggaagtaaaa taatggaacg cttcataaag
      301 tcactctcat ttaaaatcag ctggacttgt tgtttcaact cgtctggcag ttgagcataa
      361 agagcttcgc attcttcttg tgttttagct atgattgctg tacgccaata ccgggctttt
      421 ccctgagtcg ttcgaatggt atctgctaac caattcaatt tgatatcatc tgttgttcct
      481 tggatgagca aaggaaggtc tccttttctt gctgttgttt ctacttgatg ttctttcaca
      541 ataaaatgat tagcaaaatc agtgatttct tttgtggagc gatagcttgt cgttaattgg
      601 aaacgagtga cttcacggtc cacgaaaagg tcttccaatg atccaacgat cgtttcattt
      661 ccaaagacat tttgatttag atctccacag aaagtcaaat ttgcttttgg gaacaaggct
      721 tgcagcaatg ccacttgtga tggtgcgaaa tcctgcattt catcaataaa gatatagcga
      781 gctttgtgtg taaccgtcac tggatatgtt cggcgcatca gtaaaaagaa tagtacagca
      841 tcctcttgac gcaattctct ttgacgcaag tgtgtacgaa cagttgtcaa atgttcctgc
      901 cacctggtct cgctgatttt atgtcgtgtt agaagagaaa gtggcacagt ttgtagaaag
      961 tggagatatt gtttcgttat attgacaaat tgataattca tgatccctcg atggattcga
     1021 cggaatcttt tacgtacgat ttggttggcc agttttttac gtaatgaacg ctccttttgt
     1081 tctgtatatt caagatttgg atctgttgcg tatagttctt ggagctgttc ttccgccaga
     1141 tctttgaccc attgctgacg tgtttcgtct ttttgcagac cgcctaattt tttcaggagc
     1201 ttcgtttgta atagagaaag tctatgatgt aaaggtaatt gttcatttgt ttctttatac
     1261 cattggcgaa tcgattcctt tgataggatc gtacggccat ttattttcat atcacggaac
     1321 aagggaccgt agtttgtgat gccttggata tagcgatcaa tctgatcgac taaggtcaat
     1381 ccgcttttca tcacttgtat cggatctttt tcccctagca aaaaaccact ttcttgttgc
     1441 tgttcatcta gtaaagaaaa ctctggcagc agctgcacca aataattttt gaatgtctgt
     1501 gtaggcacgc cgctttcacc taatgaaggc aatacagtcg agatgtaatc agaaaacaaa
     1561 tggttgggtg aaaatagaag aacgttttca gcctcgagcc acttgcgatt atgatacaac
     1621 agaaaagcaa tacgttgaag caaagcggat gtctttccac ttccggcgat tccttcaatc
     1681 agcattactt tacttgttgt gtcccgaatg attgcatttt gtgctttttg tatcgtagag
     1741 acgatgtttt tcatatggtt gcttgatgat tcatccaata tctctagcaa aaagtcatca
     1801 ttgattactt ccgacgtatc taccatagat aaaatacgtc cttcttggat tttaaattgt
     1861 cgtttcaact gcagatcgac atctattcta tctgtttcag tttcataaaa tgcgggacca
     1921 atctcacctt cataataaag attggcaatt ggtgcgcgcc agtcaataac gattgtttct
     1981 tctgccagat ctcttaaaga agcgatacct agatataatg tttctgtttc acggtcttct
     2041 ttaaagtcga tacgggcaaa ataaggacta tctttcatga cagacaacgt ttgcaggcgt
     2101 ttttgctgtg cttcaagagt atggtatttc aaaagcaatt cctgctcatg ttggcgatat
     2161 tcaacagcag attcataaaa agcttcattg gaaccagctc gaatcttgtg attagctgtg
     2221 tcttttgtcg aatgattcat ttcctgagtc aattcttgtt gttttgtatc cagcatattt
     2281 tgttcgatct ggattagttc gatcgtttca tccacgtgcg tttgttctag ttgtttctca
     2341 ttcatcgcat gtttgctcct tccgccgtaa ataacgaatt aaaagtatag catagttttt
     2401 cgaaaaaagg tactttttaa ctgatgaatc gaaaaagtaa tccttgctga atatttttgt
     2461 ttgtagaact gatgaatttt ggaaacgctg atagtgatag ttgttttcaa ttaaggccta
     2521 ttgttttaaa aagtgctagt caaggactat gcttaagatg aaagaggcag ctggaaattt
     2581 attactaacc tctctaagtc acataaaaga ggagaaaaaa caatgacaca tttcaaaaaa
     2641 tttttactta ctttgcttat tggtgggata gctttattaa gtgaatttgt ttttcaccag
     2701 ccggcccttg catttgcgat tgtcgcattt actggaggga ttctcgcctt cttgatgttt
     2761 ttggaaatga tcaagacgtt acgttcagga agatatggcg tagatgtatt agcgattacc
     2821 gcaatccttg caacattagc cgtcaaagaa tactgggcta gtttgatgat attgatcatg
     2881 cttacgggcg gagatagtct agaggattac gcgaataaac gagcgggtaa agaactgcag
     2941 tctttgttag ataacacccc aagaattgcc catcgattca aagaaggaaa attgcaggat
     3001 atagtaatcg atgaaataaa tattgatgac cagttactag tcaagcctgg cgaacttgta
     3061 cccgcagatg gtcgagtgac caaaggagaa tcttcggtcg atgaatcttc tttgacaggt
     3121 gagtcccgcc cggaaaccaa gaaacctgga gatcagatta tgtcagggtc tttaaatgga
     3181 gacggatcga tccaaatgat cgctgaaaaa cgtgccgcag acagccagta tcaaatgatt
     3241 attaaactag tcgaagaatc aaaagaaaag cctgcccgat ttgtccgttt agctgatcgt
     3301 tatgcagtcc ctttcacgtt gattgcttat ttgatcggtg gtatttcttg gtggttctca
     3361 aaagaccctt tacgatttgc tgaggtactg gtagtggcat caccttgtcc actgattcta
     3421 gctgcgccca ttgctttagt tgcaggaatg agccgttcaa gtaaaaacgg gatcgttgta
     3481 aaaactggaa caacgattga aaagctcgca cgtacaaaaa cgattgcttt tgataaaaca
     3541 ggaacgctta ccaaagggat attggaagta gaggcgattc agccagtcaa tggaataaaa
     3601 aaaagaagag ctacttctgt atgcagctag tgcagaacag gaatctagcc atatcttagc
     3661 tcgttcgtta gtgaacaacg tgaaaaacag ccaactgtat cctgttagtc aactgaaaga
     3721 agtgaccggt caaggaatcc aagcagtagt caataatcat gaagtaaaga ttggacgtgc
     3781 ctcttttgtt tcagctgaag aaatagaaac gtccaataca gcaatctatg taacaattga
     3841 tcgatctttt gcaggaacga tcctattttc ggatcagatt cgcccagaag cagcaaagac
     3901 aatcaaggag ctaagaaaac ttgatgtgaa aaatttgatg atgatcaccg gggatggatc
     3961 tgccattgct caaacgattg cagaaaaagt ccaattagat tctgtacatg cccgtagctt
     4021 gcctcaagat aaactgacca ttttagaaag tatccctaaa gatcaaagac ctgttgcgat
     4081 ggtaggagac ggcgtgaatg acgcaccagc acttgctgct gcagatatcg gtattgcaat
     4141 gggggctcat ggttctacag ctgctagtga aagtgcagat gcggtcatct tgaaagatga
     4201 tctcttgcgt gtagcaaaag cagtagtgat ttcaagagat acgatgagaa tagcgaaaca
     4261 gtctgtactg attggcatct ttatttgtat ttttttgatg ttaattgcaa gtacgggact
     4321 aatccccgca ttattcggag ctgttttgca agaagtcgtg gatacggtct ctattttgag
     4381 tgccttgaga gcaaaaaaag atccagccat ttttcctgat tcagtaccca agacaaacat
     4441 agtatgatag aatcgagttg aaaaaagaac gatcagaaag aggagagagc attatgccat
     4501 tcgtacatgt cgaattagta gaaggtcgca gtccggaaca attagaaaat atgatgaaag
     4561 atatcacgga agctgttcat aaaaacacgc aggcaccaaa agaacacatc catgtcatca
     4621 tcaatgagat gaaaaaagga acatacggtg taaatgggga atggaaaaaa taagtttagt
     4681 ttcccacttg atttttctat ttattagtgg ttaaataaac acataattga ttgatgagaa
     4741 agccaactga atttgagagt cttcgataag agaggaaaag ctaggctgaa acttttccag
     4801 gagaaaacca aaggatgacc actttcgaaa cccttgttga agcaagggcg gtcggaccgt
     4861 tacaacgctt gaggaacgca tttgcgtttg aacattaggt ggaaccacga acttttcgtc
     4921 ctagtatctc ttttcaagag atgctaggat ttttttgttt ttcagcttga tgatctgatc
     4981 aatcaaaaca tttcaaggag gaaactatta tgataaaaat tacttttcca gatggtgcag
     5041 tcaaagaatt tgagtctgga acaacaacat tggcaattgc tgaaagcatt tcaaaaagct
     5101 tagcaaaaaa agctttagct ggtaaagtga acggaaaact cgttgatttg actcgtccaa
     5161 tcgaggaaga tgcaagtatt gagatcatca caccagacca tgaagatgca cttggcttag
     5221 tccgtcattc agcagctcat ttgatggccc aagccatgcg tcgcctctat ccaaatatcc
     5281 actttggcgt tggaccagct atcgattcag ggttttacta cgatacagat aatggacaaa
     5341 accaagtaac tgcagaagat ttgccggcaa tcgaagcaga aatgatgaaa atcgtcaaag
     5401 aaaatctgcc gatcgaacgc cgtgttcttt caaaacagga agcattagaa atttttgcaa
     5461 gtgatccgta caaagtagaa ctgattagtg aattgccaga agaagaagtg attactgctt
     5521 atcaacaagg cgaattcatc gatttgtgtc gtggaccaca tgttccatcc actggccgta
     5581 tacaagtatt taaattatta tcagtggcag gtgcttattg gcgtggaaac tcaaacaatc
     5641 aaatgatgca acgtgtatac gggacagcct tttttgacaa gaaagctttg aaagaatata
     5701 tccgtctccg tgaagaagca aaagaaagag accatcgtaa actaggaaaa gaacttgact
     5761 tgttcatggt ttccccagaa gtaggttctg gtttgccatt ctggcttcca aaaggggcaa
     5821 caatccgacg cacgatcgaa cgttatatcg tagacaaaga agttagtcta ggttaccaac
     5881 atgtatatac accaataatg ggagatgttg agttgtacaa aacatctgga cactgggatc
     5941 attatcaaga agacatgttc ccgccaatgg atatgggaga tggcgaaatg cttgttcttc
     6001 gcccaatgaa ctgccctcat cacatgatgg tctacaaaaa cacgatccat tcttatcgtg
     6061 aattaccaat tcgtatcgct gaattaggaa tgatgcatcg ctatgaaaaa tcaggtgctt
     6121 tgtcaggttt acaacgtgta cgagaaatga cgttgaatga tggtcataca tttgttcgtc
     6181 ctgatcaaat caaagatgaa tttaaacgta cgcttgaatt gatggtggca gtatatgctg
     6241 acttcaatat tacagattat cgtttccgct tgagctatcg cgatccaaac aatacagata
     6301 aatattttga tgatgacgca atgtgggaaa aagcccaagc aatgctgaaa gccgctatgg
     6361 atgaattaga actagattac tttgaagcag aaggcgaggc agccttttac ggaccaaaac
     6421 tagatgttca agtcaaaaca gctcttggta cagaagaaac attatcaact atacaattag
     6481 acttcttatt gccagagcgc tttgatttga catatgttgg agaagacggg gaaaatacgc
     6541 atcgtccagt agttatccac cgcggaattg tttctacaat ggaacgtttt gtcgcttatt
     6601 taacagaagt atacaaaggc gcttttccaa catggttggc acctatacaa gcaacaatca
     6661 tccctgtttc tgttgatgca catggcgatt atgcttatga aatcaaagaa cgtttgcaaa
     6721 tgaaggggtt gcgtgtagaa gttgatgatc gtaatgaaaa aatgggctat aagatacgtg
     6781 cctctcaaac acaaaagatc ccttaccaat tagttgttgg agacaaggaa ttggaagatg
     6841 caacagtgaa cgtacgtcgt tatggaagta aagaaacagc tgtagaagat ttgaatatct
     6901 ttattgatgc tatggaagca gaagtcaaaa attacagcag agaaaactaa taggtaaatt
     6961 tcctgtaaaa aagtagtaga tagaaagcaa tccgaacaat agataacagg tattcctcaa
     7021 agacacagat gctcaataga atctgtggga ttgatgggat aagatattct gttgggcgga
     7081 ttgttttttt gttttttatc cagtatacga ccgtgaataa gggtctgata tggtatactt
     7141 acatagacta tcattaaaag ggtggaggaa cttatgagcg taattagtcg tattgaaagt
     7201 atgatggaag attattcttc agcagaaaaa aagttggcca actacattat cggtaatatt
     7261 gagaaggtcc ctacaatgac agctaatgaa ttagcagaag cttccgggtt gagtgcgccg
     7321 acagttgtac gtttttccaa aaaaatcggt tttcaaagtc tgacggactt caaaatcacg
     7381 atttcaacag aactacagac tggaatcgat gaaggtttta gtgatataga agcaaatgag
     7441 tccttttact ctatcaaaaa caaacttggt aacaatgcac aagtagcgat aaaagaaaca
     7501 gtagatattc tagaagaaga aacgatccaa aaagtcgtag agtgtttgga atcggcagag
     7561 actgtttttt tatatggggt tggcgcctct tctttagtag tagaagatat tctgcaaaaa
     7621 tggtctcgtg tcggtaagcc aatcattttt gaaaaagata tccacgtctt gctcccgcaa
     7681 cttgtcagca atgagaaaaa gaaagtgctt tggcttgttt caaactcagg tagaagtgca
     7741 gatgtagtcg cattagccga gctggcgaaa agcatgaata tcgaaatcat tgcgttgacg
     7801 caatttggaa ataacccgct tagcaagata gccgatgtac tcgtacaaac atctagacca
     7861 aaagaaatca cgaatcgaag tgcggcaact aattcacttt tggcccaatt tgctacgatc
     7921 gatatcattt tttatttcta tatggctaaa aacgaaaagc ttagcgaaaa agtgaaaaaa
     7981 acacgagaag caatccaaga atattttatg gattcgaaat aaaaacctta ctatttttta
     8041 tataagtttt ttagaataac aaaacgtagt tttccacaaa taaatg
//
LOCUS       DAZKCY010000112         8084 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000112 DAZKCY010000000
VERSION     DAZKCY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.113"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..333
                     /locus_tag="ACG8XC_002277"
     CDS             46..333
                     /locus_tag="ACG8XC_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191949.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            749..2287
                     /locus_tag="ACG8XC_002278"
     CDS             749..2287
                     /locus_tag="ACG8XC_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFP9191950.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2284..3249
                     /locus_tag="ACG8XC_002279"
     CDS             2284..3249
                     /locus_tag="ACG8XC_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9191951.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3236..4558
                     /locus_tag="ACG8XC_002280"
     CDS             3236..4558
                     /locus_tag="ACG8XC_002280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9191952.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4621..4725
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4799..5542
                     /locus_tag="ACG8XC_002281"
     CDS             4799..5542
                     /locus_tag="ACG8XC_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9191953.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5557..6549
                     /locus_tag="ACG8XC_002282"
     CDS             5557..6549
                     /locus_tag="ACG8XC_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9191954.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6561..7298
                     /locus_tag="ACG8XC_002283"
     CDS             6561..7298
                     /locus_tag="ACG8XC_002283"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9191955.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7288..8010
                     /locus_tag="ACG8XC_002284"
     CDS             7288..8010
                     /locus_tag="ACG8XC_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP9191956.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         2656 a         1374 c         1731 g         2323 t
ORIGIN      
        1 tatttttggc tgtttctttt tatttatcaa ataaggagtg gaaaaatgcg agcagcagac
       61 ttattgacaa ttcttacaaa atcacccaaa aaaacagagt atcaaggagt ttttttggct
      121 caagaacaaa aactggtgcc tgtgacaatg gtccagataa ataaagcaaa tcaatttatc
      181 tttttatttg aacacggcaa agcaccactt gctatgaaag acatctttct ttttttgatg
      241 aagaaccgtg agaaagctgt ttattatcag tcaaaagaag aactcattcc tctttatgga
      301 gtgaaagaga tagaaaataa agtggtgata taagtaaaaa taagaaaaca agacgtttat
      361 gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag tgtcatgata cagtataata
      421 ttcgttgaac aggccaataa aggtgttctc ccccacgaga tttctacttt attggctgtt
      481 tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa
      541 tttcgtatag atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga
      601 ctttgttgct tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg
      661 ttcgtgctgt aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata
      721 cttaaagaat aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag
      781 aaaaggcgag ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct
      841 gattgcagca ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc
      901 agaagtctta ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg
      961 tttacttatc ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta
     1021 cgctttgctt ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat
     1081 tgaagaagca gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct
     1141 tcccatcgca ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg
     1201 tacggctact ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg
     1261 tattgaccga aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc
     1321 aattatattc agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat
     1381 tactttagca gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt
     1441 taaaaatgaa accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca
     1501 aatgtataaa gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt
     1561 tggcaagacg agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga
     1621 gttcacaggt actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga
     1681 tttgacaaaa gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat
     1741 gacattatta aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt
     1801 tgcgaacgaa catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa
     1861 agcgggattc acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc
     1921 atatggcttg aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat
     1981 caataatgga gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta
     2041 cgatctagtg actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct
     2101 gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag
     2161 cggaaaaata acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa
     2221 aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga
     2281 tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc
     2341 atcgatgacc taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc
     2401 ggtagtggaa aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga
     2461 gacatctact tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat
     2521 atgggatatg tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt
     2581 gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa
     2641 cttcttcaaa aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg
     2701 tctggaggag aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg
     2761 attttgatgg acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa
     2821 ttggttttgt ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg
     2881 gaagaagcaa tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa
     2941 tgtgacacac cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt
     3001 gatgaaccga aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc
     3061 ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa
     3121 gtatattcgc ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt
     3181 gggtcgctga caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa
     3241 aacatatgat gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt
     3301 gaaagaagca ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc
     3361 taaccgtgga tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt
     3421 aaaacaattg agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca
     3481 aaaattcaaa cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga
     3541 agctgaaatc gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt
     3601 caacgaagaa gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat
     3661 cttaccagtg gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt
     3721 actgcctaaa gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat
     3781 tgccaatgct gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga
     3841 atttgaggcg tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt
     3901 tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg
     3961 aaaagagacg gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac
     4021 agaatcttta gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa
     4081 tgattatctg caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa
     4141 aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac
     4201 ggatgcaaca agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc
     4261 taaacttgct gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga
     4321 gatggatctt accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga
     4381 attgaccttt gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga
     4441 cgaattgatc gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga
     4501 tagctatatc atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc
     4561 agcatgataa ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa
     4621 tacctgtttc tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg
     4681 aacctgtcag ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta
     4741 tgtcgtgccc atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat
     4801 gaagaaaaat ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt
     4861 tgtcatagtt tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc
     4921 attagcggca gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt
     4981 gcttggtttg gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg
     5041 gaagttttta gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat
     5101 ggtgttaggg ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct
     5161 tgttgtcttg ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt
     5221 atcaaaagag caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg
     5281 aatatataaa ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac
     5341 ttattgtgtc agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta
     5401 ttacatgata cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg
     5461 tgtcattcta ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta
     5521 tcgtatgctt tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta
     5581 ccgctactgt tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa
     5641 ataaaggatt tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact
     5701 ggcatctacc tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc
     5761 atcgaaccag gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt
     5821 ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc
     5881 aaagcaattg ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt
     5941 aacatccatt caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt
     6001 gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca
     6061 atggtaggaa gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg
     6121 tactttgaag gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc
     6181 ccattgaaag aattagatga acggttagat tactatactc cggtaattat tacgaatgat
     6241 caactgatca aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc
     6301 tatcaggaag ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa
     6361 gaaaatgatc ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat
     6421 gatcctgaga attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg
     6481 caggaaaacg gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt
     6541 ataaaatagg agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa
     6601 acctgaaaat attagaagac atccatttca ctatccctaa agataccttt acatcgattg
     6661 tcgcgccaag cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta
     6721 tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta
     6781 gttatatgcc gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt
     6841 tatatcaaaa aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata
     6901 tctttgggtt aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc
     6961 agcgaacggc cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac
     7021 catttggagc gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc
     7081 ctaaaaaagc aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt
     7141 tatcagacag gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag
     7201 tgcctgaaaa acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga
     7261 tttatcagtt attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg
     7321 agtcagagtt tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat
     7381 tcgccagaag aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt
     7441 tacggtatcc atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg
     7501 aaaaaagcaa ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg
     7561 actacacaaa aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct
     7621 gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct
     7681 aatcactatt tgatccattg gtattcttct ttggactatc aacaagacta tattgattta
     7741 ggctgttatt tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct
     7801 aaagcagtcc caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg
     7861 gctttagggg ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag
     7921 acaatcgctg agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac
     7981 ttggaacgtt tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac
     8041 ctctttttgg ctttctttgt ataaaagtaa actgtttgat taat
//
LOCUS       DAZKCY010000113         7999 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000113 DAZKCY010000000
VERSION     DAZKCY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.114"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            383..571
                     /locus_tag="ACG8XC_002285"
     CDS             383..571
                     /locus_tag="ACG8XC_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP9191957.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            649..1662
                     /locus_tag="ACG8XC_002286"
     CDS             649..1662
                     /locus_tag="ACG8XC_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9191958.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1729..2763)
                     /locus_tag="ACG8XC_002287"
     CDS             complement(1729..2763)
                     /locus_tag="ACG8XC_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9191959.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2878..5103
                     /locus_tag="ACG8XC_002288"
     CDS             2878..5103
                     /locus_tag="ACG8XC_002288"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9191960.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5121..7004
                     /locus_tag="ACG8XC_002289"
     CDS             5121..7004
                     /locus_tag="ACG8XC_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFP9191961.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6985..7959
                     /locus_tag="ACG8XC_002290"
     CDS             6985..7959
                     /locus_tag="ACG8XC_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFP9191962.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2581 a         1459 c         1697 g         2262 t
ORIGIN      
        1 agttagtcta cattattttg gcgatttgcc aataaggact gttgagttgt gcaaaatcaa
       61 atctgggtca gaatgaacgt cattctgtct cagatttttc gttatttcaa tttgattaac
      121 tgattgatca aaaagatttt tattttcata ttctcaaatg attcaacact aaatatacat
      181 gtttgacgcg gaaattatta ttcgattttg gcggcatcag gggggatgac gacgtagcag
      241 acccattatt ttactcaaga aaatgtccgt gcctattatt tagaatacga tagtgaacgt
      301 gctgggagtt ttgttctttt agagaaagtc tctgaagata aattagttgt cttagatttt
      361 gactgtaacg aaaaatggcg aaatggaaga gcaccagcaa gtgattgacc ggatctacga
      421 agcgacacga tatctgcctt tggatcgttt gtgcttaagt cctcaatgtg gatttgcttc
      481 aacagaagaa gaaaatgttt tgtttgaaga acaatggaaa aaatttcttt tgtcaaaagc
      541 attgcagaag aagtttgggg aaaatcatta aatcatctag gagagcttga taggaaataa
      601 cactatggta taatcagaat gcttaatact aactactgag ggagccttat gaaaaagaaa
      661 attcttaaat ttatcggatg gttcttcgga atcattcttg ggatcgcgtt tgcaatcttt
      721 cttgccttcc agctttcccc cagacctgga gcattcttga tcaaccatat gttttcaaat
      781 actgttcaaa tcactgataa aaaaacgttc gatcaagcag aaaaaaacgt ggcgaaaaaa
      841 acagatttga cgtatgagtc caagtataaa gagaatactt atgacatcta ctatccaaaa
      901 aacagcaagg ggcctgttcc tgttctattt tgggtacatg gtggaggatt cgtcggcgga
      961 gataaatcgg gtgtcaaaga atttgcgaca aaattagttt cagacgccaa catcgcagtg
     1021 gttgcgatga attatgaatt agcgcctgat tcagaatacc ctaatcaagt tcttcaagtg
     1081 aatgaactga tcaaagattt attagctgaa gaaaaagacg acaaattgct agacatggaa
     1141 caattatttt ttggtggaga tagtgcaggt tcacaaatcg cattgcaata tgcagctata
     1201 caaacgaatg atgcgtatgc taaacaaatg aaaatcaaac agctacttcc aaaagatagt
     1261 ctgaaaggaa ccatttctta ttggggacct gttgatttga agctaacagc acagcagcat
     1321 tcggatgatc gatttatgaa gtttttcgtc aaaaccgttg cgtggtcgtt gattggaaca
     1381 aaagattgga aaacgagtga gcaattgaaa gaagctagtg tggctgacca tgtttctaag
     1441 gaatttccgc caacgtatat aactgacgga aatgcatact cattccagga gcaaggtcaa
     1501 gcattagaaa atcggttgac agaattaaag gtacccgtgc aatccttgtt ttatacagat
     1561 acaaaaaaag agattaccca tgaataccaa tttgattaca cgctaaaaga atcgcaaaat
     1621 tgttaccagc agacattgga ttttgtgaat aagtataagt aagaggttgt gaaaaagccg
     1681 tttcgttttg agtatgaaga aacgaagcgg cttttcacaa cctctctttc aatacctttt
     1741 tgttcttggg ataaaacttt ccctaacaat caatcgtgca ggataagtca aaatgattgg
     1801 ttcgtcccgt ataccgtcaa ttcgctccaa tgccattcga acagctgctt gcccaattga
     1861 tttcgcattg atttgaacac tggtcaagcc aggggagagg tttgcgattg tctcgatatt
     1921 atcaaagctt gctacaacaa tctcttctcc aatttggata tttttttgtt tcaatgcccg
     1981 atagacacca attgccattg gatcgctccc gacaagaatg gcagtaggaa gaggggattc
     2041 attttctaat agattagtcg caagtacctc accttgtttt tctgtccagc cacctagatg
     2101 ataagacata tatttttcta agtgatgatc gatcataaaa tgctgatagg cacgcatcct
     2161 cttttcattt atagaaatct gcttcccacc gaattcatcc atatcgactt tatatccccc
     2221 gatataggct attcttttat gaccattttc taagaacaat tccagtacgg ttttagtcat
     2281 ccgttcaaga tctgcataca ccatatcgac ctcttgctgg atgtcagggt tatccacgac
     2341 gactatttgc atgttttgtt ttaacaaggc ggtaatcgat tctttcgtaa cagtcccaac
     2401 aacgatcacc gcacctaact gatctagatc ttcccattgt ttaggattat cagatagatt
     2461 atagattcga ttcatcccta aatgcagacg tcgagcttct tcttccatcc ctactcgcaa
     2521 atgtttgtaa tagatatcct gttcttcgct cgctttgccg atcgtagaga tcacaccaat
     2581 cgaaggcatt ctctttttga tatatttacg agtactgatg tcataatcca acgcagcagc
     2641 tgcctctact atccgttgcc gagtagtctg agccacatgg atcgtctgat cgttattcaa
     2701 cacacgggaa actgtagcag gagagacatt tgcctttcgt gcgacatctc taattccaac
     2761 catttttaca cactcctcaa aaaaataaaa aatttttatt gacaaaattg tttagtaaac
     2821 agtatacagg aattataaaa aaaagtaaac ggtttccgtt ttgttgggag tgaaatgatg
     2881 gatattacgc atagaaaaac agataaaaat ttcatcttat ttgatgaaaa gagccgagtc
     2941 ttccatttac gaaatacatt tctttcttat ctgataaaga tagaagaaag caatgtattg
     3001 gcgcatattt attttggcaa accagtcaag cagtacaaag acaataaaaa ttatccccga
     3061 agagatcgtg gattttcagg aaatgttccc ttaaatcctg accggtctct gtcgaaagat
     3121 acactaccgc aagaatactc ttctcatggc agcatggact ttcgaacgcc cgcatcgatc
     3181 attcagcgca agaatggatc agacttactg gatctgagat atgacagcca ttatattaca
     3241 gatgggaagc cagagatcga aggcttgcct caaacttatg tgatggacaa gtcagaggca
     3301 caaacgttgg tgatttcact taaggataga gaaacagcga tttatttcga tttgttctat
     3361 accatattta ctgatagagc agtgatcaca cgatctgtaa agataagaaa tgaaaccgga
     3421 gaaacaatca aactagaaaa agcggctagt ttccagctag actttgccca tactaggcga
     3481 tttgatgaag tcattgcttt gccaggagca catgtcaatg aaagacagat ttcacggcaa
     3541 tctgttttat ctggaacaaa agtatttgaa agccgtcgag gaacgagcag tcaccatatg
     3601 aataacttta ttgctttagt tcatcatcat acgacagaaa atactggaga agcgatcgga
     3661 cttcaatttg tttattccgg caaccattct ttcgaacttg aaaaagatca gatcaatcaa
     3721 ttgagagtcg tcggtgggat caacagccat cgtttttcgt gggaattgaa tgcaggccag
     3781 tcttttcaaa cacctgaaat gatcctttct tactcaagtc agggattgaa caaaatgagt
     3841 cagatccacc atgagttgct acgtgaaaga atcgcaagag gtcgtcatca atttgctgaa
     3901 cggccgattc tggtcaataa ctgggaagcc acttactttg acttcaatag tgaaaaaatc
     3961 aaggcgatca ttgatgaagc aaaggagcta ggcatcgaaa tgttcgtatt agatgatggc
     4021 tggtttggaa aaagagatgc ggacaattct tcattaggag actggttcga gtatgaaggc
     4081 aagttgacaa atggattacg tgaaatagca gattatgctc atagcaaagg attgaaattt
     4141 ggtttatggt ttgaacctga aatgatctcg gttgattctg aactgtatcg aacacatcct
     4201 gattttctta tgcaggagcc aggtagaatg ccttctgctt ccagatcaca acatgtactg
     4261 gattttacaa ggttagatgt tcggcagacg attgagaaac aaatgagaaa gattttagat
     4321 acgattcctc ttgactacat caaatgggat atgaaccgtt cgctttcaga tgtctattcg
     4381 attacccttg acccacagag acaaggagaa gtagcgcatc gttatatgct cgggctttat
     4441 gaactacttg aacatttgac aactgattat ccggaaatct tatgggaagg ctgttctggt
     4501 ggaggtggac ggtttgatgc cgggtttatt tattatatgc cgcagtcatg gacaagtgat
     4561 aacacggatg cagtggagcg tatgaaaatc caatatggaa caagccttgc ttatccgatt
     4621 tcgtccatca cagctcatgt atcagctgta ccaaatcatc agaccgacag atctacttcg
     4681 ttgaaaacaa gaggagaaac agctatgagc gctgtttttg gttatgaact ggatttgaca
     4741 aagctgtctc ctgaagaaaa aaagcaagta aaagaccaaa ttatatccta tcaaacgatt
     4801 cgtccggtga tccagtatgg ccattattat cgtttggcga gtccttttga agaaaatatt
     4861 gccgcatgga tgtttgtgtc tcctaaacaa gatgaagcta ttgtgttttt agggaggata
     4921 ctagcttcag ctcaaccagc gttccatgaa gtatatctga tggggttaga tgatgaggca
     4981 ctttatcagg aacagacctc gaagcggata ttttcggggg ccgaattgat gacagttgga
     5041 ctttacttcc ccgattttca aggtgatttc caaacagaac tgcttcattt caaaaagtta
     5101 tgagagagaa ggaaaaaagt atgaaagcaa atataaaatg gttagatgat ccacaagttt
     5161 ttagagtgaa ccaatcaaat gcacatagcg atcatgtttg ttatgcaaca agggaagaag
     5221 tagaaaccac atcctccctt aaacagtctt taaatggatc atggcgtttt tgttattcgc
     5281 caaatccttc ggtacgacca aaaacatttt ataaaaaaga ttttgatgcc tgttcttttg
     5341 atgaaatcac tgttcctagt catattgaat tagcaaatta tgacaagatc cattacatca
     5401 atacgatgta tccgtgggag ggacatgtat ttcgccgtcc ccctttcacg acaagccctg
     5461 agaaagaggc ggaagggatc ttttcagctg ctaaagacaa tccggttggt tcgtatttga
     5521 cgacttttcg attagaagaa ggtctaaagg gaaaacaaat ctcgattcat tttgctggtg
     5581 tagaacaagc aatgtttatt tggttgaatg gtcattttat cgggtatgcc gaagattcgt
     5641 tcactccgtc tgaatttgat ttaacacctt atttggaaga agaaaatatt ctagctgtgg
     5701 aggtttacaa aagaagtaca gctgcattct tggaagatca ggactttttc cgattttttg
     5761 ggatcttcag agatgtcttc ttagttgctc gaggacaaaa ccatttagaa gatctttgga
     5821 tcaaacctcg cttagatgac aaatgtcaaa atggcaaact tgaagtagat ttgtcttttg
     5881 agcaagtagc caaaaacagt cagctgaatg tagcgatact agatcctgat gggaaggaaa
     5941 tctatcaaca gcaatatccg ttaaaagagc aaatgacgat tcgcatcgat ggatttgaaa
     6001 atgtggcact gtgggggcat tttcagccca atttatataa gcttgaattg gagacgatag
     6061 acgaagaaaa aagagtaacg gagtatattg actattcctt cggttttcgt aaaatcgaaa
     6121 tcaaagataa aatcatctac ttcaatggaa aacggatgat tatcaacggg gtaaatcgac
     6181 atgagtggca tccgaaaaaa ggacgcgcca tcactataga agatatggaa aaagatttgg
     6241 ctatcataaa aaatgcaaag atcaacggcg tacggacatc tcattacccc aaccagatca
     6301 cttggtatta tttatgcgac caagcaggaa tccatgtgat ggcggaaacg aacctcgaat
     6361 cccatggctc atggcaaaaa atgggcgcat ttgagccttc ttacaacgta ccaggtgcag
     6421 tacctcagtg gcaggcggct gttcttgatc gagcaaaaac gaatttcgaa acattaaaaa
     6481 accatccaag tattcttttt tggtctcttg gaaatgaatc ttatgcggaa gaaaacattc
     6541 gacaaatgaa cgattacttc aaaaaagtag accccgatcg acttgtccat tatgaaggga
     6601 attttccaaa tccagcattc gaagattcga tttcagatgt aaaaagctgc atgtatgcgc
     6661 caccagaaga gatcattgac taccttgaaa ataaagcgga aaaaccgttc atcttatgtg
     6721 aatatatgca tgacatgggg aattcgttag gtggaatgga ttcttacatg acgttgcttg
     6781 atcgttatga aatgtaccaa ggaggctata tatgggattt taaagatcag gcgatccaag
     6841 tgactgatcc aatcacaggc agagaagtga tgcgctatgg cggtgacttt gacgataaac
     6901 caagtgacta tgaattctca gggaatggga tcgtttttgc agatggacaa gaaaaacccg
     6961 ccatgcagga ggtaagatat tattatgaaa aatacagtaa ataaaagtca tatggatacg
     7021 gaaaaagttg caatcgtctt cggcgactgt acattaggtg tcaaatcggg gaatacgcat
     7081 tatatttttt cttatacaag aggcggactg gaatcgctca ataaaaacgg aaaagaatgg
     7141 ctataccggg aaacacttcc agttttttgg cgagcaacga ccgataatga cagaggtagc
     7201 ggatttggat ttaaatccgg tatgtggtta ggagccggat tgtatccaaa agtcaaagaa
     7261 atagaagtac tggtggacaa taaaaagatc gatttgccga ttgctccagt aaataatcaa
     7321 tattcaagta atgaatacgc tgaaacggtt gaaatcattt ttacactgga aatcaacaca
     7381 gtaccaaaaa cccaagtgac aatttcctac caaatcgctg gagagggtaa aatgcttgtc
     7441 aatatgcatt attatggaca aaaaggatta ccggaattac ccgcattggg tttaaggttc
     7501 attatgccaa cgaaagcaaa attttttgag tatgaaggcc tatcagggga aacatatccg
     7561 gatcggatgg ctggtggtgt cgaaggtatt tataaagtgg caggactccc cgtttcccca
     7621 taccttgttc cccaagaatg cggcatgcat atggaaacga aatgggtaga actaacaaga
     7681 aatcaaacgt tggataatac ggataaagat caagaagatt tttcgttgcg atttgaacag
     7741 caggatacgc catttgcttt ttccgcttta ccattcaaag cagaagaact ggaacaggca
     7801 acacatatag aagaactacc gccagaaaga cgaacggttc ttacgatttt tgcaaaagta
     7861 agaggagtcg ggggtatcaa tagctgggga gcggatgttg agtcacctta ccgaatcagc
     7921 agcgaagagg accatgaaat cggctttatt attcagtagc aatctttaga ttattatttg
     7981 aaaaacggag tttacaaga
//
LOCUS       DAZKCY010000114         7883 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000114 DAZKCY010000000
VERSION     DAZKCY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.115"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..481)
                     /gene="yajC"
                     /locus_tag="ACG8XC_002291"
     CDS             complement(101..481)
                     /gene="yajC"
                     /locus_tag="ACG8XC_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP9191963.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(578..1723)
                     /gene="tgt"
                     /locus_tag="ACG8XC_002292"
     CDS             complement(578..1723)
                     /gene="tgt"
                     /locus_tag="ACG8XC_002292"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP9191964.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1941..2639
                     /locus_tag="ACG8XC_002293"
     CDS             1941..2639
                     /locus_tag="ACG8XC_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP9191965.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2807..5446)
                     /locus_tag="ACG8XC_002294"
     CDS             complement(2807..5446)
                     /locus_tag="ACG8XC_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9191966.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6065..7096)
                     /gene="queA"
                     /locus_tag="ACG8XC_002295"
     CDS             complement(6065..7096)
                     /gene="queA"
                     /locus_tag="ACG8XC_002295"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP9191967.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7289..>7883
                     /locus_tag="ACG8XC_002296"
     CDS             7289..>7883
                     /locus_tag="ACG8XC_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9191968.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDE"
BASE COUNT         2227 a         1710 c         1454 g         2492 t
ORIGIN      
        1 ttgagcaata tatcccatgc tcttatctct ttcttttttg aataaaaaat gaggtaggac
       61 atctttctgc cctacctcgt ctttaaataa gctttgatac ttatttttct tccttctctt
      121 ctgtctcttc cagcgtttct gttgtttcag tttttacttc aactgtctca gctggttctt
      181 ctgttgtttc aacagtagtc gttgcatcat ttgttacgac agtccctggt ttgaccgttt
      241 tgattgctgc acgatcgtac tcaaggaaga tcccttcgca gtctaaaaca actgtccgtt
      301 tgtcattgtc gatttctgaa acaactccat gtaatccgcc gatcgttacg actgaatcac
      361 ctggtttcat gctatctaac aagttttgac gttcttgctg ttgtttcttt tgcgaacgtg
      421 acataaaaaa ccacattgct aatagcgcga taaacatcac aagcattggt aatgttccca
      481 tttttttcac ctcactaatt ctgtatctac aaaaaacact ttagcagaaa aatgctgata
      541 acactagttt tttacttaat ttttaaaaga ttttccttta gaagcttttt gcgttttctt
      601 tattgaaccc gtattcttca aaaaaggctt ctctaaattc aagcaaatta tcatccatga
      661 ttgcttgacg tacttgtttc attagattca acaagaagta taaattgtgg taagaagtca
      721 aacgaatccc aaatgtttca tcacatttga tcaaatggcg gatataggca cgtgtatagt
      781 ttttacatgt ataacaatca catttctcat caattggacg gaaatcatgg gcatatttcg
      841 catttttcac tactagacgg ccttgagaag tcatacatgt gccatttcga gcgattcgag
      901 taggcaagac acagtcaaac atgtctactc cacggataac accatcgatc aatgaatcag
      961 cagcaccgac acccatcaag taacgtggtt tgttttcggg gatcaacggt gtagtgaatt
     1021 ctaatacacg attcatttct gatttaggtt ccccgacaga taacccgccg attgagtaac
     1081 cagggaaatc catcccgact agatctttcg cgctttgacg acgaagatct tcaaaccctg
     1141 ctccttggat gattccaaat aatccttgac ggtctggatt agcatgagct ttcaatcctc
     1201 gttctgccca tcgagacgta cgttctactg attttttcac gtagtcgtaa ctttcatcga
     1261 atggtggaca ttcgtcaaaa ctcatcatga tatcagagcc caatttattt tggatgttga
     1321 ttgctttttc tggtgataaa aacatacgcg aaccgttcaa gtgatttttg aaatgcacgc
     1381 cttcttcttc gatattccgc atatcactta atgaaaaaac ttgaaagccg cctgaatctg
     1441 ttaaaatcgg ttgatcccag ttcataaatt tatgcagtcc gccagcttct tctactaagt
     1501 cttcaccagg gcgtagccat aaatgatatg tattacttaa aataacgcca gcacccattt
     1561 ctttcaactc ttctggggac attgttttta ctgtagctaa tgttcctact ggcatgaaca
     1621 ttggtgtggg gaaagttccg tgtggcgtga tcaattcccc taaacgcgcg ccagtatgtt
     1681 tttctttctt gattaaacga tagcgaatgg caggttgcgt cattcatttc cctactttct
     1741 atataaaata tcttgacaac tcccaccatc ataatcgttt caagtgcttt ttgcaagttc
     1801 cctcggcttc cttctacttt atttaatatt ctttttttta tccttttttt atgaataaca
     1861 aaaaaaagaa aaacattatg acagaaatat tttttgatac atggtatatt ggtgttatta
     1921 aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt aaaacgccaa gcaaaagaga
     1981 gtctaaaagg acgttgggga caagctgttt tactaaatct aatccctaca ttgattggta
     2041 ttgctactat tcttcttatt atgattccta ttatcttgtt tgccaccacc atatatgatg
     2101 gatcagcgag cgtctcagaa gcgacaactt caggaggcgg tagttcagga ggcggtggcg
     2161 gaattatttc cagcatcatt agcgctttgt tcatgagcgg tatttcttgg acttatcttg
     2221 atattttacg tggacaaaaa gatcagatcg aaccattcaa agatgcattt cgtggattca
     2281 aaggtttgtt tatcggcgga gtgatcagct tagctgtgct gatctcgatc tttacaacat
     2341 tatggggttt gctattagtt attcctggaa tcattaaaag ctattcttat tctcaaagct
     2401 attttattta ctacgatatc gtccaagaaa caggacaaac acctaaagta ctggatacga
     2461 tcactgcaag tcgaaaattg atggatggtt ataaaggaca acttttctgg ttagatcttt
     2521 catttatcgg ctggcatatc ttagcattag caactgctgg tattggttat ctatggctca
     2581 gcccatacat ttctgcaaca aaagcagcat tttatgaaca attgccaaaa gatatttaaa
     2641 agagagtgtg acttgtgacc gagggggtgg caggagaaca ccgtttatcg agaggcagta
     2701 aaagaaacat tcagcccatt agcaaaaagg aggtgtgaca acattcatgt cacacctcct
     2761 ttttgtatag ttccagtaaa aagaaaatca acttagaaac agttaatcat tcctcttctt
     2821 ctgcttccgg taatcgatgg aacttgatca gttcattcac cgcgattggt acgatagata
     2881 aaatccctac caagaaccag tgattcaaac tgatgtgtac taggccgaat agttcttgcg
     2941 taaatggcag caaagcaaca gccgttgtaa tagctaatgc cagtaacgcc atttctacca
     3001 aagctttatt ggtcgctaaa ttgactttga agatcgattg gctgctgcgt acattgaaca
     3061 catgcagaat cgatgaataa gctaagatca agaatgcaac agtttgaccg acatgctgac
     3121 ttgcgtctac ccatgggctg atagtatcga cataactacc taaataataa ccaaacaagg
     3181 ttacgatggt gaagacccct gcatttatgg caattttttg caataaacct cgagcaaaga
     3241 tcccttcatt tttcggaatg ggtggctgat ccatgatgcc gtgttccgct ggctctcttc
     3301 ctaaagcaaa tcctggaagt ccatcagcaa ctacattgac aaacagtagt tgcacagctg
     3361 taaatggtgc tccccatcct aacagcatcg caatcaaaac aataaagatc tccgaaatat
     3421 tacagcttag caaaaagttg atagctttac gaatattctg ataaaccgaa cgtccttgac
     3481 tgacagcgtc tacgatcgtc gcaaagttgt catccgtcaa aatcatatct gcggcacttt
     3541 gcgccacatc tgttcctgtg atccccatgg cacacccaac gttacttgct ttcaacgctg
     3601 gtgcatcatt cacaccatcc ccagtcatcg ctacgactgc acccgtacgt tgccaagatt
     3661 tgacgatccg gatcttgtct tctggcgtta cccgagcata gacagagaga gctttgacac
     3721 gcgcatctaa ttcttcgtct gataattgat gcagttctga tcctgaaagt gcttctgacg
     3781 gatcattcaa gatacctaat tctttcgcta tggcactggc agtcaccaca tgatctccag
     3841 tgatcatgac tgttttgatg ccagcctttt tcgctcgctt gattgctcct ttactttctg
     3901 gacgtggtgg atcgatcatt ccgatcagac caatcaaacg taagtctttt tccagtgctt
     3961 cagaagaaat atcttgcggt tcttcgtcat aagtagcata accaacagca atcactctca
     4021 gtgcccgttt accaaagcga tcattaacga tcgccgcttg ttctacatcg cctgaactaa
     4081 agcgcggagc aagaacatcg aatgctcctt tggtgattga gatgtatttt tcgcccattt
     4141 ggtgaactgt tgtcatcatt tttcgttcag aatcaaaagg aagctcgttt actcgtggat
     4201 atttttcttc taattctgcc tttgtatgat aattctcttc aacagccctt acgatggcta
     4261 cttctgttgg attacctttg ataacaaggt cgtcatcttc ttgctcgacc gtcacatctg
     4321 tacatagtgc agccattttc aatatttcca tcgcgctgtt cgtcattgaa tcttcgatat
     4381 tcgttacttc atcttctttg ctccataccc ggcggacgcg catttcattt tgtgtcagcg
     4441 ttcctgtttt gtctgagcag ataacattag cggttcctag cgtttcaact gctggcaact
     4501 gtcggatgat cgcatgtttc ctcgccatct tctgcacacc gtatgcaagt gtcaacgtaa
     4561 caataacagt caaggtttcc ggtacagctg caacagccaa ggaaatcgaa gtcatgaaca
     4621 tctcaaggag tggttctcct tgcagttcac caatcacaaa tactaaagcc gcagccgcta
     4681 aagcgatcat actgattcgt ttgcctagct gattcaatcg gacctgtaaa ggtgtttttt
     4741 gcatagtatt ttcgttcaaa agatccgcga tcttacccat ttctgtagcc atgccaatgg
     4801 cagtgacgat cgctttccca cgacctgcag caaccgtaca tcctttaaat accatattaa
     4861 gttggtcacc taatgactca tcttcttcag caataaatgc tgcatctttt tcaactgctt
     4921 cactttctcc agtcaacgcc gattcttcta cacgtaattg tgatgcttcg atcaaacggg
     4981 catcggcaga aatagcatca ccggactcta aaacaagaat atcacccact accagttgct
     5041 ctgactcaac agtggtcact ttgccgtctc gtatgacagt tgccgtttgt ttattcatat
     5101 cttgcaagga ttccagtgct ttttctgcgt ttccttcttg gacaatcgcc aataccgagt
     5161 tgatcacaac gatagcgatg atcagcagtc cttcaaataa atccccatgt tctgtagcaa
     5221 atgcggtata aaaggaaatc accgctgcta caagaaggat gatcgttgta aaatcggaca
     5281 agcttcgaaa gaattttttg atcatcgatt ctttcggcgc ctcatcgaat ttattcaatc
     5341 cctgcttttt tcgccgttca tccacttctt gtgaagacaa cccagtatct ggatctgctt
     5401 ctgttacctt ctttacttct tctattgtct gcttgtattc ttccatccta ttcctccttt
     5461 aaaatcttcg tacaaaaaaa ggtacttatt ttactataac atcctttttt tatttacttc
     5521 aaataaaggg cttatcgatt atttgttaag aaagcgtaaa atttaaagtg cttatatttg
     5581 caaacgctac caaaggatgc tattatatag atgtaaggaa ggaggatacc atcaaataga
     5641 cagaagaatt tctcgataga acagtgtaag caaacaaagt tgttagaagt acaggtttca
     5701 cccagacatg cgtgtagagt acaaacctat aaatagtgaa tgcgtatgga tgtttggatc
     5761 acgaagtatc cgaaacaaga agtaatgcga tacgtaacaa ttgaataacg taaacgtctg
     5821 aaaattgaat aggaacaaga gatgtttcaa atgttgaaga tgtaacgaaa acaaaatttt
     5881 tgtttaacat gaagaaattc aacaccgtgc tttgcatgta tgatggaaag ttacaatcat
     5941 ttctgtttat ttgaatcctt ccgaggtaca agaaagccga gcttatcgag agctcggctt
     6001 tttctttgtc ataaaatctt tgtaaagctt ttttgttatt caaaactagc ggcttttcat
     6061 aaccttattt cacgaacatc gcatctccaa aactaaagaa acgatagcgc tcttcaatcg
     6121 catgctggta agcagataaa gtcaattctc ttcctgcaaa ggcactaact aacatgacta
     6181 aagtcgattt tggcaaatgg aaattagttg agaaagcttc taccactttg aattcatagc
     6241 caggcgtaat gaaaatatca gtccatccgc tgtctgcttt gatttctcca ttaaatttcg
     6301 ttccaatcgt ttctaatgtt cgaattgaag ttgttccgac agccacgatt ttcccgccat
     6361 ttttacgtac ttggttcaat cgctcagccg cttcttccgt caaacggtag aattcactat
     6421 gcatatggtg ttcttcaatt ttatctacgc tgacaggtcg gaatgtacct aaccctacat
     6481 gtagtgtcag atagactaat tcaacaccct ttgcttcgat ctcatcaagt aattctttcg
     6541 taaaatgcaa tccagctgtt ggcgctgcag cagaaccatt ttctttcgca tagacagttt
     6601 gatagcgatc tgggtcttcc agacgttctt tgatatatgg aggcaatggc atttctccca
     6661 atgattcaag aatctctaga aaaattcctt catactcaaa gtcaatgatc cgtccgccgt
     6721 gttccaattc ttctttcact actgcttgca atctgccatc accaaaagag attcttgtcc
     6781 caactttggc acgtttcgca ggtttgatca atgtctccca cgtatctcct tgtgtattcg
     6841 ttagaagaag gacttctaaa tgcgcaccag tatctggttt ttctccatat aaacgagcag
     6901 gcagaactcg tgtattattc ataactagcg catcaccagg ctgtaactct tcaatgattt
     6961 catggaaatg tttatcttcc atctctccag ttttccgatc caaaacaagt aaacgagaat
     7021 gatcgcggtc ttttaatggt gtttgtgcga ttaattcttc cggcaagtca aaatcaaaat
     7081 cttctgttgt taacatatcg tcactcactt tctatcatgt gcttcatcat tctatcatgt
     7141 ttttcgttct gttggcaaaa tcagcacttt ttcacttcta ttttttgttt tcctaaataa
     7201 aaaaactacc ctatttgtat tttttcattg ttttaggcat actagtaata gatagttctt
     7261 cattgaaaat agaaaaagag gtggcaccat gattgaattt caaaatgtgt caaaaattta
     7321 taaaggtgga aaagtcgctg tagaggacgt caatctttca tttgaaaagg gtgagttcat
     7381 ttgttttatc ggtacgagcg gaagcggaaa aacgacgacg atgcggatga tcaaccgaat
     7441 gactgatcca acacaaggga aaattttgat tgatggaaaa tccatccaag aaattgatcc
     7501 agtggaattg cgacgtcaga tcggctatgt tatccaaagt attggattga tgcctcacat
     7561 gacgatccgt gaaaatatca ctcttgtcca aaaattgttg aaagtcagtc aagaagaacg
     7621 gaataaaaca gctgaaaaaa tgattgatct tgtggaattg ccccgtgaga tgttagatcg
     7681 ctacccacat gaattatctg gtggtcagca gcagcgtatt ggagtcgttc gtgcccttgc
     7741 agcgaatcaa gatatcatct tgatggacga gccattcggt gcgcttgatc caatcactcg
     7801 tgattccttg caagaccttg tcaaagattt acaagagcgt ttgggaaaaa cgatcgtatt
     7861 tgtaacccat gacatggatg aag
//
LOCUS       DAZKCY010000115         7600 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000115 DAZKCY010000000
VERSION     DAZKCY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.116"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACG8XC_002297"
     CDS             complement(138..428)
                     /locus_tag="ACG8XC_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191969.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACG8XC_002298"
     CDS             complement(546..806)
                     /locus_tag="ACG8XC_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191970.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACG8XC_002299"
     CDS             complement(943..1356)
                     /locus_tag="ACG8XC_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFP9191971.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACG8XC_002300"
     CDS             complement(1812..2297)
                     /locus_tag="ACG8XC_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191972.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACG8XC_002301"
     CDS             complement(2436..2651)
                     /locus_tag="ACG8XC_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFP9191973.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACG8XC_002302"
     CDS             2875..3675
                     /locus_tag="ACG8XC_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9191974.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACG8XC_002303"
     CDS             3694..5562
                     /locus_tag="ACG8XC_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP9191975.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACG8XC_002304"
     CDS             5555..6046
                     /locus_tag="ACG8XC_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP9191976.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACG8XC_002305"
     CDS             6034..6588
                     /locus_tag="ACG8XC_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP9191977.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="ACG8XC_002306"
     CDS             6606..>7600
                     /locus_tag="ACG8XC_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP9191978.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAZKCY010000116         7367 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000116 DAZKCY010000000
VERSION     DAZKCY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.117"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8XC_002307"
     CDS             complement(99..1229)
                     /locus_tag="ACG8XC_002307"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP9191979.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8XC_002308"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8XC_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9191980.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8XC_002309"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8XC_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP9191981.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4549)
                     /locus_tag="ACG8XC_002310"
     CDS             complement(3485..4549)
                     /locus_tag="ACG8XC_002310"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9191982.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8XC_002311"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8XC_002311"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9191983.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8XC_002312"
     CDS             4880..5692
                     /locus_tag="ACG8XC_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9191984.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTKTLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8XC_002313"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8XC_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191985.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(6502..6732)
                     /locus_tag="ACG8XC_002314"
     CDS             complement(6502..6732)
                     /locus_tag="ACG8XC_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191986.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
     gene            complement(6716..6913)
                     /locus_tag="ACG8XC_002315"
     CDS             complement(6716..6913)
                     /locus_tag="ACG8XC_002315"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP9191987.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..>7367)
                     /locus_tag="ACG8XC_002316"
     CDS             complement(7059..>7367)
                     /locus_tag="ACG8XC_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9191988.1"
                     /translation="LGVKGQNLLADDSFWQIEAFYLAQCAYNTTLMFSPDRIIFGGGV
                     MKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGIIGGLALARKAVRNKQP
                     "
BASE COUNT         2269 a         1477 c         1132 g         2489 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaacca aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaa
//
LOCUS       DAZKCY010000117         7288 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000117 DAZKCY010000000
VERSION     DAZKCY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.118"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..461
                     /locus_tag="ACG8XC_002317"
     CDS             141..461
                     /locus_tag="ACG8XC_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9191989.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            913..1113
                     /locus_tag="ACG8XC_002318"
     CDS             913..1113
                     /locus_tag="ACG8XC_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9191990.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1260..4049
                     /locus_tag="ACG8XC_002319"
     CDS             1260..4049
                     /locus_tag="ACG8XC_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFP9191991.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4096..4623
                     /locus_tag="ACG8XC_002320"
     CDS             4096..4623
                     /locus_tag="ACG8XC_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFP9191992.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4644..5834
                     /locus_tag="ACG8XC_002321"
     CDS             4644..5834
                     /locus_tag="ACG8XC_002321"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9191993.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5874..7172
                     /gene="asnS"
                     /locus_tag="ACG8XC_002322"
     CDS             5874..7172
                     /gene="asnS"
                     /locus_tag="ACG8XC_002322"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP9191994.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
BASE COUNT         2420 a         1338 c         1559 g         1971 t
ORIGIN      
        1 aaaaaagagg ttgtgacgtg gtcacgacct ctttttgacg attatgatac aaaaaacatc
       61 atttatgaac aaaatgtgaa taacgggcgt tcttgagata tgattttaat tgggaaaaac
      121 ttatcgataa ggaggtcttt atgaaaaaaa gactgttatg gtgtttgctg tttctatccc
      181 taagtttaac aagtcagcca gtttttgctg aagaaacaca gcggtctcct attacaggca
      241 cacgtctgta caaaaatgtt tatgtacaga caacagatct tgattctgcg attcctgaaa
      301 cagaagaacg gtctttgcag ccaagatttg gttacttttg gtactgtccg acctgtgatt
      361 atgaaagttg ctggcatttc ttacattcta cagctgcagc ttttgctttt actcatactc
      421 agaaatatga tggacatcat cctgttattt atgaggtata gatatgagca aaaaagaaaa
      481 actaatctcg ttgatcgtat tggtttgcct tgttcttctg gtaactgtca taggctatca
      541 gaaataccat gagaacatca gacctactaa agaatcttcc ccagcctcga ccgttgaaac
      601 agaaccgata agtagtcaga aagaataatt tttttagttt aaagaagaac atcagagcaa
      661 aaaaacacca actgaacaat atgtcgttta ttttaaaaaa agttcggttt ttcactttat
      721 gtaatcgctg agaaaacttt ttttaataaa aaaagtcgaa aaactctagc ttttttgtaa
      781 acggtatgat atactatagg agtagttttt gttcggtaat ctgtggtagc ttgtttcaaa
      841 taaacatctt ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg
      901 aggattttta ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga
      961 ttcatcactg cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac
     1021 ggattcaaaa ctttagaaga aggccaagca gtaactttcg atgttgaaga aggccaacgc
     1081 ggacctcaag caacaaacgt tactaaagct taattaacct atactaaaaa ataaacgccc
     1141 agacatttgc gtgtctgggc gtttttgtct attttcttta aaaagcgatg agagtatttt
     1201 tctgcacttt gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt
     1261 tgaaaccaag aacttatgca gtcgttgata tcgaaacgac tgggacgaat ccaaaagaag
     1321 atcggatcat ccaatttggc tgcgtcatga ttgaatcagg tcggatcact tcacgttttt
     1381 cgatcgatat tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca
     1441 cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg atttacaatc
     1501 ttttggcaga caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc
     1561 atgaactcat gcgatgcgga cttcctagtt tgaagatacc aggcatcgat acagtagaac
     1621 ttgcacaaat tttcttgccg acggaaccaa gctttcgctt agctgatttg tcagaaagcc
     1681 ttggatttat tcatgagaat cctcatcagg cagatagtga tgccgaagtg acgggtcaac
     1741 ttcttttgct gatcgaagaa cggatgagaa aattgccaat catcacgtta gagcagattg
     1801 cgcgattaag tagacacaca ggtatggata cgagtcgttt catttatcat gtgattgaag
     1861 aaatgaaaga aaaaccagag ccacttgatc cctcattaga aatcgtcgat gggctagctt
     1921 tgcagaaaaa agaagtggaa ctattcacat ccgtccatta tggcgagcgg acatatccaa
     1981 gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga
     2041 acagactgat gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga
     2101 tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct gctgccattg agttatttgg
     2161 ctacacctga aaaacccgct gtcatcagta ccgtttcgct agttttgcag cagcagatca
     2221 tagaaaaaga cattccattg ctgaatcaat tgctagatca gcccatacaa cctgtaatta
     2281 tcaaaagcta tcgccactat attgatttac agcgttttaa aggcacactt gtggaaccgc
     2341 cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt agtttggctg acgcaaacga
     2401 agaccggtga tttggatgaa ttacatttaa caaatttgaa ccattcattc tttgcagata
     2461 tcgcgcacag aggtattgga tttttagcaa gaaatcagtc attttacgaa caagactttg
     2521 ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat catgcgtttt
     2581 taatacaaga aacacagcga aaagaaccat tgcttccagc tagtccattt ttgttgatcg
     2641 atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga ttaagtatta
     2701 actatttcca taaacaaatc cagcaattag aagaaccaaa tcagctgttt gaccgaatac
     2761 aaagctattt accgaaagaa catgaagcct tacgctggtt atcgatttat cgagaagaac
     2821 tttctgcgct atgcgaagca cagatttgta tggcagaagg attaatgagt ttagtgagcg
     2881 gcaagcaacc taacggacag tatccagaag aagtcatgat gacaaaagag atgttggatc
     2941 agctgccact ggaatctggg acagccattg atcacctgca gcttttttat caggagatcc
     3001 agcagctaca gtcacaaatg cgtctgactt ttgagattat ccaagaaagc tggacaaacc
     3061 agatgcggat cgatttagca agtttatata gcttatttga tcaattagac aagcagtact
     3121 tgctgatgga tcaatggctg gaagactggc gagatcattt gatccattgg ttcgttcctg
     3181 ggaacacagc gcagcagatg atttttcatc tccatgattt tcaagcggct atgttgccgt
     3241 cgactgtttg gtatcctcga tatgatcgta ttctttatat tggaggaacg ctgaagatcg
     3301 gccctaatcg acactatctc tctaagcggc ttggcattga agcagcacct cttaaagtga
     3361 ttgcctctcc ttatgattat gagaaacagg caaggatcta tgtgccaaaa gaggcacctg
     3421 ccgtccatca agtcagcaat caagcatata tcgattattt agagacagca cttacaggtc
     3481 tgatcgaaca agaagatcgg ccaatacttg tgttattcac ttctcatgat gttttgcaga
     3541 aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata ctagctcaag
     3601 ggtttggcgg aagccgggag aagcttttga aacgcttcat tttgtcagaa aaaagcgtat
     3661 tgtttggcgc tgacagcttt tgggaaggtg tagatttgcc tggggatgct ttgcagatcg
     3721 ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa gctaggaacg
     3781 cttatttaga atcagagggg atcaatcctt tttaccagga atccgtacct aaagcagctt
     3841 tgaagttacg ccaagcatta ggtcgcttga taaggacgga gacagatcgt ggcgtattgc
     3901 ttattttgga tcgaagacta gtcactgcaa aatatggtgc acggatcatc catgcattac
     3961 cgaaaaaagt accgatcaaa gagctagcta tggaggaaat ctatcaggac atccatgaat
     4021 tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct tttttcataa ttaaaatctg
     4081 ctataatgaa aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag
     4141 gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat ttttattagc ggtcatcgtg
     4201 atcagctcga ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa gaaagaagca
     4261 gtagaaatcg caaagaaata tgcaaatctt gaggaagccg atcattttta ttggtttact
     4321 cgaaagaaga catacttttc actgacaggt acaaatgata aaggaaaaga aatcgccgtt
     4381 atcattccta aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa
     4441 aacgaagcaa aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct
     4501 ttagggatgt atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa
     4561 ctgaattatt acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt
     4621 taaaaggtaa aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt
     4681 ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa agcaaaaggc agagatgtgc
     4741 tgagtttgac tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa
     4801 tcgaagcaat ccgcaatggg aaagctagct attatacacc aacagcagga atcccagagc
     4861 ttcgacaggc aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac
     4921 aaacagtcgt aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg
     4981 acccgcaaga tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa
     5041 aactggcaga aggacttcct gtctttgtga aaggggaaga atccaatagc tggaaagtta
     5101 ctgttgaaca gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc
     5161 catcgaatcc gactggaatg atttatagca agaatgaact tcaggcaatc ggtgaatggg
     5221 cagtaaaaaa tgatattttg atagttgctg atgatattta tggtcgttta gtttataatg
     5281 gcaatgagtt tactccaatt gcaacgattt cagaagcaat caaaaatcag accatcatca
     5341 tcaatggcgt gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggga
     5401 attctgagat catcaatgga atgattgctg ttgcttccca gtcaaccagt aatccgacag
     5461 cagtcagtca atatgcagct gttgaagcat tgaaaggtga gcaagataca gtagaagaaa
     5521 tgcggaacgc ctttgaagaa cgcctgaata cgttatatcc gcttgtggca gaactgccag
     5581 gtgtttctct tgaaaagccg caaggagctt tctatctttt tcctaatgta aaagaaacgc
     5641 tacgcatgtg taaatatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag
     5701 gggtagcgct tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg
     5761 ctactgatcg cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta
     5821 aaagtcaaaa ttaatcctga agaaattcaa ggaatataaa aggagagtca tacgtggaac
     5881 aaattcaaat catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg
     5941 tcgcaaataa gcgatcaagc ggaaaaatca catttttaca attaagagac ggaacagcct
     6001 ttttccaagg tgtagttgta aaaagcgaag tgtcggaaga agttttccaa acagctaaaa
     6061 atctacacca agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agtcgttcaa
     6121 aatttggtta tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc
     6181 cgatcacacc aaaagaacac ggaacagact tcttgatgga tcatcgtcat ttatggctgc
     6241 gttcttcaag acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg
     6301 aattctttaa tcaacatcat tttgtcaaaa ttgatccgcc gatcttgact ggttctgcac
     6361 cagaaggcac tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc
     6421 aaagtggcca attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg
     6481 gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca tttgatcgaa ttctggatga
     6541 tcgaaccaga aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg
     6601 tagcattctt agtacaaaat gttttagatc attgcgaata cgcattagat gtactaggac
     6661 gagacaaaga agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttacgatg
     6721 atgcaatcga attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg
     6781 gttcacctca tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact
     6841 atccaaaagc catcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtaa
     6901 tttgtgcaga tatgatcgca ccagaaggtt acggtgaaat tattggcgga agcgaacggg
     6961 caacggacta tgatttcttg ctagaacaaa tccgtaatca cggtttggat gaaaaagaat
     7021 attcatggta tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag
     7081 gtcttgaacg aacagtcact tggattgctg gtatcgacca tgtacgtgaa gctattccat
     7141 tcccacgttt gttgaaccga atttatccat aagaggttgt gcgcctgccg ttcagctccg
     7201 agtcacaagg agtaatttca aaaaacagtt tttcatgttt gatgactatt gtgacttgtg
     7261 gccggagagc aacgtttatt taaaaaaa
//
LOCUS       DAZKCY010000118         7286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000118 DAZKCY010000000
VERSION     DAZKCY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.119"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..548)
                     /locus_tag="ACG8XC_002323"
     CDS             complement(<1..548)
                     /locus_tag="ACG8XC_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9191995.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAA"
     gene            complement(587..1705)
                     /locus_tag="ACG8XC_002324"
     CDS             complement(587..1705)
                     /locus_tag="ACG8XC_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFP9191996.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(1702..3801)
                     /locus_tag="ACG8XC_002325"
     CDS             complement(1702..3801)
                     /locus_tag="ACG8XC_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFP9191997.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(3852..4673)
                     /locus_tag="ACG8XC_002326"
     CDS             complement(3852..4673)
                     /locus_tag="ACG8XC_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9191998.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(4670..5479)
                     /locus_tag="ACG8XC_002327"
     CDS             complement(4670..5479)
                     /locus_tag="ACG8XC_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9191999.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFVMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(5501..5995)
                     /locus_tag="ACG8XC_002328"
     CDS             complement(5501..5995)
                     /locus_tag="ACG8XC_002328"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9192000.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6007..6507)
                     /locus_tag="ACG8XC_002329"
     CDS             complement(6007..6507)
                     /locus_tag="ACG8XC_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9192001.1"
                     /translation="MIKPMFLKVGTGIAYMKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDYEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQEIKMTILDEVHVVN
                     EEEDFF"
     gene            complement(6572..>7286)
                     /locus_tag="ACG8XC_002330"
     CDS             complement(6572..>7286)
                     /locus_tag="ACG8XC_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9192002.1"
                     /translation="INVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDESIEV
                     FTKEYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDILDGDGAANLKHI
                     LSSEMKPVEFNRLIQELIKFFSLEGIADRLNFLNPTIVMSEVETVISKYENYYHFTLN
                     GRVKLNLYMHTALMMERLFLARTEKAEEPNYDMKPDEDEFYYVSRSIFQSMEMKYNFK
                     VNAYELSLLYELLGPYIQK"
BASE COUNT         2202 a         1679 c         1228 g         2177 t
ORIGIN      
        1 gctgccatcc cctctaaaca atcttggtgg aagggacagt ttcctgaaaa attgtccttt
       61 ggatgtcgct tgatcaacat atgccccatt tccggatgac tgaacccttc cacgaaagtc
      121 ccattgaaca aggcaccacc tccgatacct gttcccacag tgaagtatgc aacacctgct
      181 aaccctttgc cagaaccagc aacatattca ccatagcaag ccgcattgac atctgttgtc
      241 cattcaatcg gaatattgaa atattgcttt agcgttccta caaaattgaa attttgccaa
      301 gcaagttttg gtgttgatgt gatatgaccg aaactcgttg atttgcattg aatatcaatt
      361 ggaccaaagc agccgacccc gatgcctgcc aattgttcct taaaaggttt aaaaaaatca
      421 atgacctgct tcattgtttc aactggagtt gttgtgggaa aactgactct ttccaaaatc
      481 gttaattcct cagtaccgac tgcacagaca aatttagtcc ctccagcttc aattgcccca
      541 tatatcatca taggttcctc ccttcgaata tgtgaaaaac acttcattaa cgcggcaagc
      601 gttctcgatc agtgaaatga cgtttagtat ttcctgaagc ttctgttgaa taccagtaag
      661 cgacactagc aacatcgtct tgccgctcaa ataagcgaac atcatcgttg ccgatttgtt
      721 gtaaagtgac cgataaatct tcggtaaaac agatggggtc ttgaagatgc caacgataaa
      781 gaccatgtcg tggcattgcg tcgtcaccaa aggcatgaac ggcattcaat tttcccgttt
      841 caaaacgatc tcttgtggca tctctatttg attggtaagg ataccctaaa aaggctgttg
      901 aatagttttt cgcatgtgga cgtccgaact catcgtactc atgaaaggcc cacgccccgc
      961 caaaataatc ttctgaacct gttgaggtta cggtcggata ctcactatcc ccatcaatgt
     1021 agaatttaaa ctcgccttcg ccccaccaat aacgttctag gcaggtaagc gcaaggtaag
     1081 tacccacgta atagccttga ccctttaatc gatcaattaa cttataatcc tcctgtaatt
     1141 tggtaatgcg ttcccgatgc caatttgcgt gaaaatatag gctattttct tccaattcgt
     1201 ccaccaaagc gtaatttatc gtataaaaaa atgaggacat atccttcgga tgtaaattct
     1261 cgatcgtaat tttagcggat gtttggaaag gcatttcgaa ataggaatta aagccccctg
     1321 taggattaac cactatcggc aacgagttca cctcgcaacg ctctccaaac ccattacaaa
     1381 agaaatcccc taaaggactt tcaacagcag ggctatccga cccatcccaa taaattctta
     1441 agattaaatc acgtaaaaca aaactgccaa attctgttcg atcacgaatc gtcatccaca
     1501 tatgacgaat tattcccgtt ccagtaatat ccgcaatcac agtttcttca ccagaagtca
     1561 gcggaattgc gccacgacct ttgcggcttg gtcctagctc gcttggcaac atcgcagctt
     1621 tacctttctc gcctgttgga ttttcgggag tgattgctcg actctggatt tttttaaatt
     1681 gggtaatatt ttttagtata ctcatctttt tctcctttga atgtgaatcg tttttccctt
     1741 ttctacgtga agaatttttt tcgtatcact tattttcaat ggcttttctg acaaatttga
     1801 aatactcaac tcggattgcg tacattcaat cgaaatgaag gcttgaccga acttcaaatt
     1861 cttccaagaa aacgttaaat tatcaacagg atcaatttgc aatactcctt ttttgaattt
     1921 caatccgaaa tattgactga tcattaggct gacaaaaaga cccgatgccc agccgcattt
     1981 gccgcagcca aagtctcgca cgacttctcc ttgttgagcc tctaagcgat acggccacca
     2041 ccaccacgaa ccgtctaaat ctgccaaaca aattagttct tctaatcggg ttacgaaagc
     2101 ttgagggttg tgcagctcgc tcattaaaac agtgataaag ccaggaaaag tggcgccgga
     2161 ttctaatccc caagtgatgc ctttgatttc ctcgctatag gttggattga aagaactagc
     2221 agaaaaggcc atggtgttct gatataaatg atctttatta tctaaaaatt gataaaaagg
     2281 catcaacgta gtgtctgatt cttctccatc atgcatcaat aaattaattt ttccatccac
     2341 aatcacatca cttaaaaaga ttaacccttg ctcaagaatc ggtttttgat agtgcttcac
     2401 tgaataggtt gttttctcct catcaccgat tccctccaaa aattgctcac caaaaggacc
     2461 atcgatagtc atttgtttac aaattgattc ctttagttta caggcaatgt ttttgtatcg
     2521 cttgctaaga ctcagttgtt ctagggcttc aaaaatttct gcaagtccaa tacaggcctt
     2581 ccaaagacaa agattgctgc cggtatgata ctttccaagt gcaaaggcgt ctgataacca
     2641 aacagatgga aaaagcattg gttcccctga ccgacgttga gctaatatgt cctcaataat
     2701 tcttgaagca ttcagtagaa cctgcggaca ccgctcaaag aattttgtat ccccagtatg
     2761 ttcaaaataa agactagcca gtaacgcact ggataacgaa ttactcagag aatgattgat
     2821 acctcctgta aattttgaac caggaaactt aacgccatat tgatcaaacc atagaaccgt
     2881 tttttgacat aactccggtt caaacatggt gaatggcaat gatgcgtaat acatatcctt
     2941 attccaaatt cgattagtag caggatagct gccccaattg ctgccgacga cctgtccctg
     3001 tcgattcata ccgaaactag aaaagctttg ataaagtgca cgacgataga gatggacaac
     3061 tctatcgttt ggcattgaca gctgcccaaa tagctgagca aaatagttca atgtttcctt
     3121 cagccatttt tcgatatcag attcctgaaa agtcattact tcttcaaaag catttgggtc
     3181 cacaaaagca atggcgaagc ttgccacttc ctgttgtgcg atcgtgtcct tatttccttt
     3241 ttgagaaatc aacacatttt gctgatccga atacttctct tgatacgaag gcacatcttt
     3301 cactgaaact tgaaggcttt cgtgtgtttc attcacaatg taaacctgtt gaacaagcat
     3361 acttaaacgc ttattagcca gtatgggagc aaaggatact acaatgacct taaacgtcga
     3421 ataatgatga atatatctag gcaaacagtc ttgaatcaca tcgacttcaa tacgatgatc
     3481 cgattctgac aaatggtaga caatcccgtt tacttcaacc tttaaggata acgaattggt
     3541 ttgagagagc tgtttattga cccaaacacc tggtttttcc tcagttagta aattttctcg
     3601 ataaaaggtc aacgtttgaa tcgtcccgca aacatcaagg tttgctaatg tcttttgatt
     3661 acttacatcg aaggtcaatc cctttacatc aggaatattc cgatagtagc tgtattcgtt
     3721 tgaagtagtc acatttccgt cgtttaactc agcattaaat aatgggtgtt tgggtgaata
     3781 aattacttca ttaatagtca tcttttccct ccttgctaaa aaaactgcag cctagtagct
     3841 gcagcttcct actatgcgat tccaagtaat gacaataaaa ttcccaagac gaccattcca
     3901 aaaataattt tgatgacgcc gactttttta tctagtaatt tgaaacagcc caatgttacc
     3961 aataacggta caattccttt aaaaatacta tcgaggattt cttggacatt cattagttct
     4021 tcgcccttca ttgaaaagct caaaacggta ttgaatttga ccatactgat cgtcattgcg
     4081 cctaccatga tcagacccaa gatactagca gcttttgtca gaatttcaat taacccgttc
     4141 gcatacagct tttcaatata tttagagcct aatgaatagc ctaaaacgcc gccatagtat
     4201 cgcatcaaat aattaggtat attgaacata actaaaaaga taattggtgc caaaatattt
     4261 ccggcattgg ctaagttgat cgctagtccc gcggtaacga cacgccaaac tccccagaac
     4321 aatgaatcgc caattccagc caatggtccc atcaaactcg ttttgatcgc gttgatactt
     4381 tccacgtcaa aaccagcttt ttcagaattt tccttctcca tcgatgccgc aatccccatg
     4441 acaaaggacg cgcaaggaat ggtcgtcgaa aaatactgtg tatgacgttc cagcgccgca
     4501 gctcgatctt cttcactttt gtagaaatga ttaatgaagg gcatcataga ataggcgaaa
     4561 ccaccgccgc ccatcgtcac cggattcacc gaagcataca tagtcatcga gcgccaaaac
     4621 atcttccgaa ccaatttttt ctcatcttta gaaattccat cagctaattt cattgaaaga
     4681 aatcctcctc ttcttggtca agcgttgcat ttacagcagc atcgctttga actgcatgaa
     4741 cagatatatt ttttagttgt agatctcttt gggctataac aattgcaata atcgttccaa
     4801 ctgcggcaat tgcaatcaat ggtaactcga gatacgccat aacaacggcc ccaaagaaaa
     4861 accacacagc aatattttta ctccaaagca tccgtaacag catagccatc ccaactgccg
     4921 gcagcagatt tccagcgact gttaatccgt tcatgatgac ctctggaata ttatccaaga
     4981 cgcttttgac aacatctgct ccaaataaaa cggcaaagaa tggaataaga gcataaagcg
     5041 catagtggac aaaccacata acgaagtgca gattgatgat tcctttttga tcaccagatt
     5101 ttgcaaattt gaaaaattta gcagccatcg gtccacatac aaaagaaaaa atcatattca
     5161 ttaactgtaa tcctaatact gctagtggaa agacaatggg aataatcgca tccacgtttc
     5221 ctcccatttt gattgcgaac gctgtagcca acccagcagc caaagaaggc tccgccgtac
     5281 tagagattcc gatattgacg acacccataa aaatggcttc tagtgacgcc ccgacaatca
     5341 accccgtctg caaatccccc atcaacgctc ctgctaacgg agcaacaaca atcggacgac
     5401 tcagcatgga catcccgaac atttcatgtc cagcaactcc aataaaaacg gccaaagcga
     5461 ctacaaatgc atcatacata atttttctcc ttcctttttt ctaaaaatca ctcaaaatag
     5521 agatagctga acgtttttcc atcgccggtg taggctgaaa aacggtatct ggataaagtt
     5581 caactaattc ttttaagtct tgaatttctg acgtcgttaa catcacttcc tttgataagg
     5641 ttttcttatc ttccgatggc ggtgtcattt tcccagcatt ccccacgttc attaattcga
     5701 tttccgttgg gaaagctttc gcaactttta ccgcatcttt gacattaggg actaaaacca
     5761 tcaagcgcat gtccttcgcc cgcggatctt ttaacaaatg aatcgcgtca tcaactgttt
     5821 taataatgac tttgacattc gatggtgcag ccatcttcaa tgtcatcttt tgaatatcgt
     5881 tggtagccgc ctcatcgctg gctactacta acccggttaa atttagactc tttgtccaag
     5941 tcatcgctac ttgtccatga attaatcgtt catcaattcg cactgcttta atcatttttc
     6001 ttcctcctaa aaaaaatctt cctcttcatt tactacatgt acctcatcaa gaatggtcat
     6061 ctttatttcc tgttggcatt tatttaccag ttcctgaata tcctcctttg ttaattcctt
     6121 ggttgccaat actatttcga gcagaattgg cagattaaag ccagacaaaa tagttacagc
     6181 aatctctttc tccaaaaagg ttttggtaat cttctgattg acacttccgc caaataaatc
     6241 ggtaaaaaca aaaatctgat cctgctcgtt cacctggttt acaaatacat tcaactcctg
     6301 ttcataatca cactcatcca cataagcatt aatcaccgta atctcttgaa ccttccccgt
     6361 cagtaattga atactgctaa gaaagcctga agctaagtga ccgtgagtcg ctattagaat
     6421 tttcttcata tggttaattc ctttctttct gttcactttc atataagcaa ttcctgtgcc
     6481 aacttttaaa aacattggtt taatcacgtt aattcacata aaaaaaagtg tctaacctga
     6541 aaaaatcaga ttagacactt ttttagacac tttatttttg aatatacggt cctaataatt
     6601 catagagcag cgaaagctca taagcattga ctttaaaatt atatttcatc tccattgatt
     6661 gaaaaatact tctcgaaaca taataaaatt catcttcatc tggtttcata tcataatttg
     6721 gttcttctgc tttttctgtc cgcgccaaga ataaacgttc catcatcaat gccgtatgca
     6781 tataaagatt tagttttacg cggccattta aagtgaaatg atagtaattt tcatatttag
     6841 aaatgactgt ttccacctca ctcatcacga ttgttgggtt cagaaagttc aaacggtctg
     6901 cgattccttc caatgaaaag aattttatca gctcctgaat caaacggtta aattctaccg
     6961 gcttcatctc agaactcaaa atatgcttca aattggctgc tccatcacca tctaaaatat
     7021 catatacatt gatattagga attgaaaagg aacgtggaag atccgaagtc gtgatgacca
     7081 attttgtctt gtcaaaataa gtttcatcat tttggtcgat taattcacgt agttctctgt
     7141 attccttcgt gaaaacttcg atggactcat ccgataaatg atgacgaaaa acatccttca
     7201 gtttatccga gatacccaat cccgacatgc aggaaatgat gatgttgcta ctttgagaaa
     7261 agccttcaaa gtattgaaca ttgatt
//
LOCUS       DAZKCY010000119         7270 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000119 DAZKCY010000000
VERSION     DAZKCY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.120"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1570
                     /locus_tag="ACG8XC_002331"
     CDS             <1..1570
                     /locus_tag="ACG8XC_002331"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFP9192003.1"
                     /translation="FSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLEQVVLLADES
                     LEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLADQAKQATFQ
                     LNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEH
                     DGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTM
                     GSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNK
                     DWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYH
                     LKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVL
                     KKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIK
                     ENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENE
                     AWLQGALEVFKHEQ"
     gene            1560..2777
                     /locus_tag="ACG8XC_002332"
     CDS             1560..2777
                     /locus_tag="ACG8XC_002332"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9192004.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2790..5939
                     /locus_tag="ACG8XC_002333"
     CDS             2790..5939
                     /locus_tag="ACG8XC_002333"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFP9192005.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(6688..7270)
                     /locus_tag="ACG8XC_002334"
                     /pseudo
     CDS             complement(6688..7270)
                     /locus_tag="ACG8XC_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406632.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         2485 a         1211 c         1476 g         2098 t
ORIGIN      
        1 atttagtgcg gcagataatc tccgtagtaa aatggatgcg tcagaatata aaaattattt
       61 attgggactg attttctata aatatttatc cgatcgattg ttggaacaag tggttctttt
      121 agcagatgaa tccttagagg aatatgacac agtaagcaaa caaacaatgt tgtatcgtga
      181 attactttca gatgaagaat caaaagaaga tctgattgcc acaatcgttg atattttggg
      241 atatgcaatt gcaccagagt atcttttcaa cgtcttagcg gatcaagcaa aacaagcaac
      301 gttccaattg aacgatctaa acaaagcctt tgtgcaattg gcatctacct acaatcaatt
      361 taatgggtta tttgatgatg tggatctcca atcaaaaaaa ctaggaacag atgagcaaca
      421 acgaaatgtg acgattaccg aagtcatcaa gaaattgaat gatgtagatg ttttagaaca
      481 cgatggggac gtgatcgggg atgcgtatga gtttctgatc agtcaatttg cttcagaagc
      541 agggaagaaa gccggagaat tttacacacc tcatatggtt tctgatatga tggcgcaaat
      601 cgtaacgctc gatcaaaagg aacgacgatt ctttagtgtt tttgatccaa cgatgggttc
      661 aggttcattg atgttaaacg tacgtaacta cttaacacac ccagataacg ttaaatatca
      721 tgggcaagaa ctaaatacaa cgacatacaa tctagcaaaa atgaacttga ttttacatgg
      781 tgtcgacgcc gaagaaatga atctccgcaa tggggatacg ttgaataagg actggccaac
      841 agatgaacct tacacatttg atgcagtagt aatgaaccca ccttattcgg ctaattggtc
      901 agcagatacg actttcttag atgattctcg gtttaatcgt tatgggaaat tagcgccaaa
      961 atccaaagca gattttgcgt ttcttcttca tgggttctat catttgaagg aaacaggtac
     1021 catggccatc gtcttaccac acggagtttt attccgtggc gctgcagaag gggtcatccg
     1081 tcaaaaatta ctagaagatg gcagtattta tgctgtgatc ggtatgcctg cgaacttgtt
     1141 ctttgggaca tcgattccaa cgacggtgat tgtgttgaag aagaatcgtc aaactcgaga
     1201 tgtattattc attgatgcta gtcgtgaatt tgttaaaggg aaaaatcaaa ataaactatc
     1261 ggaagaaaac attcaaaaaa tcctcgaaac ctacgctgaa agaaaagatg tcgagaaata
     1321 cgctcatttg gcaacctttg atgaaatcaa agaaaatgac tataacttaa atatcccacg
     1381 ctatgtagat actttcgaag aagaagaacc aattgatatg gttcatgtcg gaaatgacat
     1441 caagaagata cgacaagaac agcaagtgct agaaaaaaaa cttttagaag ctatctcttc
     1501 cctgcaaaca acacctgaaa atgaagcgtg gttacaaggt gcgctggagg tgttcaaaca
     1561 tgagcaatga tacacaacca gaaatccgtt tcccaggatt tacagatgat tgggaacaga
     1621 ggaagttggg ggacttcgtt gtagattatg ttgaaaagac aagtgtacaa aatcaatttc
     1681 ctatgctaac ttcatctcaa caaaaaggga tagttttgca agaagactat tttgcaaatc
     1741 ggcaagtaac tacagaaaat aatattggtt actttgtact acctagagga tatttcacgt
     1801 tccgtagtcg aagtgataat gatgtgtttg tgtttaaccg taatgacatt attgatagag
     1861 ggattataag ctatttttat cctgtattta ctttaaaatc tgctgattcc gacttcttct
     1921 taagacgtat taacaatgga atccaacgac aattatctat tcaagcagag ggaacaggtc
     1981 aacatgttct gtctctaaaa aagtttaaaa atatagtagc aatgtttcct agtgaggaag
     2041 aacaacaaaa aatcggcacc ttcttcaaac aacttgacga cacgatcgct cttcatcagc
     2101 gtaagttaga tttgctgaag gaaacgaaaa aaggattctt acaaaaaatg ttccctgaaa
     2161 acggagcaaa agtgccagaa atccggtttc ctggttttac ggaagattgg gaagagcgta
     2221 agttgggaga attaggtaaa actcaatcgg gaattggatt tcctgatgcc gagcaaggtg
     2281 gttctgaagg tactccattt tttaaagttt ccgacatgaa taacattggt aatgagtatg
     2341 aaatgagaaa tgcaaatcat tatgtatcta atgagcaaat tgagcgtaaa aaatggaagc
     2401 caataaaaga tgtgccagcg attatatttg ctaaagttgg cgcagcaata atgcttaata
     2461 gaaaacgttt ggtcacaagc cctttcttaa tagataacaa taccatggct tatttattta
     2521 ataatacttg ggatatctat tttggtaaaa tattgtttga aactataaat ttgccaagat
     2581 actcccaagt aggggcattg ccttcttata acagttctga tattgagaat atcagtgtta
     2641 aggttcctgt aaaggatgaa caacaaaaaa tcggtacttt cttcaaacag ctagatgata
     2701 cgatcgctct tcatcaacgt aagttagatt tactaaaaga aacgaaaaaa agcttcttac
     2761 aaaagatgtt tgtctaggag ggaagaagaa tgaaaattag tcaccgtgag gaagcagaag
     2821 tcgaagaaca gttgattcgt gtacttggag aagggcataa tcagtggacg tatcgtccgg
     2881 atctaaaatc agaagaagac ctttgggtca atttacgcca aaaaattatc tcaaataatc
     2941 aagcagaact gaatgatttt cctttgaccg ataaagaatt tgaaacgatc aaaacggaat
     3001 tactgctgcg aacgaaaacc ccatttgatg ctgcgaaatg gttaaaaggt gaaaatggga
     3061 tggctcggat cacgattgaa cgggaagatt cgcaattggg ttctgtctca cttatccttt
     3121 attccaatca agacattggt ggaggaatct ctacttatga agtggtccat caaattgcaa
     3181 aacgtggaag taacatagaa gcacgggatc gacgttttga cgtcacctta ttaatcaatg
     3241 gattaccgat tgtccaaatc gagctgaaac aagtaactgc aaaagatggt gtttatcagg
     3301 catttaacca aatcaaaaaa tatgcagaag aaggtatgtt tcggaacaat atcttttcga
     3361 ccttacagtt gtttgtggtt tccaatgaac aaaccactcg gtattttgca aatgccttac
     3421 cgaaagactt gcaccctaag tttttattta gttggcgaac gaaagacaat gaaaaagtag
     3481 aaaacttata tgagttttgt aaacaagtgt taaatatccc ggatgctcat cgtttgattg
     3541 cggattatac aatcgtcagt gaagatcaag acaataaaac cctcatggtg ttacatcctt
     3601 atcaagtaca tgcgattcaa gccttgttta ttgccgcaaa taaacatcaa tcagggtatg
     3661 tgtggcatgc gactggttca ggaaagacgt tgactagttt tgtatctact aagcttttag
     3721 cacgaaaatc ggggatcgat cgaacgatca tgttagtgga ccgcaaagat ttagataatc
     3781 aaaccaccac agagtttaca aaatttgctt cggaatttaa tactgggatt tcttccggga
     3841 acgccaaagc gaatagcttg atcgttggaa cgggaagtgc caaagaatta agtgaaaccc
     3901 tgttggcaga tgccaatgca aatgttgtaa tcattacaac ccgtcaaaaa ctagatgcgg
     3961 cgttgaaata tgcaaagaaa caagaagaga aaaaaggaac caatcgcttc caaaaattaa
     4021 tgggccaaca tatcgtgttt gtagtagatg agtgtcatcg agcgttaagt gctgaaaata
     4081 tggaagaaat caaaaaaatg tttccgaaat ccacttggtt tggctttaca ggtacaccga
     4141 tttttcctga gaatcggaaa caagccaaag ggcaattggc acgtacaacc catgatcagt
     4201 atggagaggt cttgcatacc tataccatta agaatgcgct agaagatggc tctgtcttag
     4261 gatttcaagt agagcatgaa aacaccgtgg agccgacttc actagagaat aaaatctatc
     4321 ggaaattaaa agaagtagaa acatatgcag agtattcatc agaacagatc aatcgtatga
     4381 ttgatcaaat ggaacctgtc aaaaaagaaa gttatttgga cccagctgtt tttgaagcag
     4441 atgaacatat ccaaaaagtc attcataaaa tgttccgtcc agataatgcc tatacaaaat
     4501 ttgattttcg aaatggcagg ccaacgaaat ctgcaatctt aaccacgagt tccattgaca
     4561 tggccaaaaa gtattatcgg gccatcaaag agatgacgaa agagcctgat tggttaacaa
     4621 aagagtttcc caatcagctc attcgtgaag gacgaacgat ggaggatcct gattttccac
     4681 gaatcgctat tacctattca ttagaagaaa atagccgaga ttcaagtgcc caacaagaag
     4741 aaatgcaaaa aattattgag gaatataatc agtattatga tacttcctgg tcattagcag
     4801 acattgagcg ttataacgga gacatcaata atcgtttggc acgaaaaaga gcggaattca
     4861 aacaatttgg gaaacaaatc gatttagcga ttgtagtgga tcgtctatta acaggttttg
     4921 acgcccctac gattcaaacc ctatttgtgg atcgtaatct agagtatgca ggattgatcc
     4981 aagctttttc acgtacgaat cgtacgtatc ctgataagac aaaaggattg attgtgacgt
     5041 ttcgtaagcc agcgacaatg gagaagaatg tcgaggatgc gacaaaactg tattcaaaag
     5101 caaaagaaga atcaggtttg atttatccaa gttatcagga atccaaaaaa agattcaaac
     5161 aagcatatgg taagttaaaa gaggtttcat ccatggaatc cattgatgaa catacgccac
     5221 ttgaaatccg tatcgagtac gtcaaagcat ttcaagaatt gaatcgtgct tatgaatcgt
     5281 tagtgactta tgacgattac aatgatgaca tggtgaaatc tccaactttg aacaatcaaa
     5341 ttcaactttt ggaagaacaa attggcgtgt acgagaccgt gaagggttca ttaatagagg
     5401 aagaaccaga aaaagaagga gaagattttt caacgatcga attttatagt gaaaatacca
     5461 ctaagttgta tgatattgat tcaacttata ttgatcaatt actaggaact tatgctgcga
     5521 atagttcaga tgcacgagaa gaaattgaaa aagctttggc taaattgaat aaaacagaag
     5581 gagtcaaaga agtttaccga caaattttaa atgcatttga tgtggggact ttagatagta
     5641 atgaagatat ctttgtcatt aaacgtcgct attttacaca agcgagtgat gatttgattc
     5701 atgtcttttc caatgagtgg tttgtttctg agaaagaatt acatgcttct aatctgcaat
     5761 atctaccagg tgaagatcca attcctaata tgaaagcaat tatcaacagt aaggattacg
     5821 aaggatataa agccaaacat ccagaagcga agccatttaa atacccacaa gaaatgaagc
     5881 gagcatggcg taaaatgtta gatgatgagt tgattccact tgagaatgaa ttgaggtaaa
     5941 cgagaagagt taagaaaatt gtacgattca aagatggtag tcaagtggaa gtgtagaaaa
     6001 tagggataga tgtcttttat ttgttcacat tttataacga agtatttaag acagctattg
     6061 aataatgttt cctccacctt tttcattcat ttaccccacc ccgttgttat atgaccgtgt
     6121 ttcgagagaa accaataaaa agcataggct aactaggggt tcctttttct tttgattttt
     6181 tggaatccgc ttaagtgaaa tgtatctgaa atgaaacaga gataaagtga ttttttgctg
     6241 taatgacaag gttttagctg tttcagatac catgtaaccc aaaatagttt attgagaaaa
     6301 taggagagta tttgatacta actagtagga aaaaatgaga taaaaagtaa atggaatcat
     6361 ttcaggtaat ctcaaataaa aaatatacag gcctgtggca ctttacctac gggactgtat
     6421 attttttact ctcagcgggc ttgttttgac tccgtgtttg actccgtcgt attgatttta
     6481 aatgatattc gatgaccaat taataggtcg tttttgaaat ttttgtctct atatgactca
     6541 gtttgataat tattgataga tcgatttggg tctatatcga acaagggaag attgcaaaag
     6601 tcgaagctta aaggattgat attaaaggat taagaggtaa aaaggtatcc ccataatatc
     6661 ctctgactcc gtttttgacc tcgcttatca tccgttcatg taagaatcaa ataattcgac
     6721 tgtttcattt tgccgatttt ttgatacttt aatataaatg tccatgattg ttttagaaga
     6781 agaatgtcct agcctatctt gtacatcttt tacttgagca cctgcttcaa agagaagggt
     6841 agcatgagtc tttctgaagc catggacttt gattatcttc atatctggga acttccgtgc
     6901 tactttatcc atgacatggt tagggtagcc tttgtaaagg aagtcccttt ttttattccc
     6961 tttgatagtg ctgattacag gtatatcatc tatattatct atccgaatcc ctctagaaag
     7021 gttcttttat gttacagaac gtttccattt tttagaatac gtaaggttcc attatcaatt
     7081 gaaattactc cctttgattt gtctgttttt gtaggattaa tacatagaga gccctcggat
     7141 agttctacta acgttttgtt gaagcagata gtttgattat cgaaatctat gtctctacat
     7201 gtcagtccga gtatttctcc ttttcgttcc cctgtataag aagctattct aaagaatact
     7261 aaagcacgct
//
LOCUS       DAZKCY010000120         7265 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000120 DAZKCY010000000
VERSION     DAZKCY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.121"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..1727
                     /locus_tag="ACG8XC_002335"
     CDS             96..1727
                     /locus_tag="ACG8XC_002335"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9192006.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1730..2809
                     /gene="uxuA"
                     /locus_tag="ACG8XC_002336"
     CDS             1730..2809
                     /gene="uxuA"
                     /locus_tag="ACG8XC_002336"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9192007.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2883..4310
                     /gene="uxaC"
                     /locus_tag="ACG8XC_002337"
     CDS             2883..4310
                     /gene="uxaC"
                     /locus_tag="ACG8XC_002337"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9192008.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4394..5041
                     /locus_tag="ACG8XC_002338"
     CDS             4394..5041
                     /locus_tag="ACG8XC_002338"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFP9192009.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5161..6537
                     /locus_tag="ACG8XC_002339"
     CDS             5161..6537
                     /locus_tag="ACG8XC_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9192010.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            6547..>7265
                     /locus_tag="ACG8XC_002340"
     CDS             6547..>7265
                     /locus_tag="ACG8XC_002340"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9192011.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLF"
BASE COUNT         2498 a          952 c         1489 g         2326 t
ORIGIN      
        1 gcaagtttat tcccttttta tattgacaac ggttacaatg ggttttaaaa tataaaatgt
       61 atcaaaacta acatatcagt ttgaggaggt taattatggt ttcgattaaa aattggcaaa
      121 attctatcga agaattcaat gaaatgcaga ttctactgcc gtgttttgat gtcaaagaag
      181 ttaagcaaga aggccagcaa catccagtat ggcttcattt tggtggagga aatctttatc
      241 gaggatttca tgcagaaatt gctcaatgct tagcagatca aaatgaatta gaaacaggag
      301 tagttgtgtg tgaaacattt gatgaagaag tagtggaaaa agcttatcat gactataaca
      361 atgacatttt ggaaattgtt atgagtggag atggtgaact caaaaaaaga cttttggcag
      421 taacggctga tagtttatat tgcaatccga agaatatagc ggactatcaa agagtggtta
      481 aatattttga agatcctaaa ttacagttta caacgtttac tattactgaa aaaggttaca
      541 gtttaaaaga ttctaaaggg caattctttt cggtagtcaa agctgatatc aaatctggac
      601 ctagcaatcc tcaacatacg atgagtattg taacatcctt gttatatagc agatttaatg
      661 ctggagagtt gccgattgcg atggtttcta ctgataattt ctcacagaat ggtcaacgtt
      721 ttcaagatag tatttttacg attgcagaag cttggcaaaa agaaggatat gtttctgaaa
      781 aatttattgc ttatttaaag gataaagaaa aagtgacttt tccttggagt atgattgatc
      841 gaattacgcc aaatccttca gagaatgtaa aggaagatct attgatgatg gggctcgagg
      901 atttagatat cattcacaca aaaaaacata ccaatattgc tgctttcact aatactgagg
      961 tagttcatta tttagttatt gaagatagtt ttccaaatgg ccggcctaaa ttggaaaagg
     1021 ctggtgtgat tatgacagat agagagacgg ttgataaagc agacatcatg aaggttacga
     1081 caagcttgaa tccacttcat actgctttgg cagtaacagg ttgtctgcta ggatatcaat
     1141 caatttcagc agagatgcag gatgatgatt tggttggttt aatcaaagga ataggttatt
     1201 tagaaggttt accagtagtt gaaaatccag gcattattga tccaaaacaa tttattgatg
     1261 aagtaattaa taaacgtttg cctaatccct atattccaga tacaccacaa cgaattgcaa
     1321 cagatacttc tcaaaaagta gcgattcgct atggtgaaac aattaaaaag tatgtcgaaa
     1381 aagacggaaa tgcagaaaat ctaacattta ttcctatcgc tattgctgct tggttacgtt
     1441 atttactggc agtcgatgat caaggggaaa attttgtgcc aagtcctgat ccgttattgg
     1501 tagagttgca ggataaatta tcagtattga aattaggtga acaagatcct tcaaagattc
     1561 attgtgcggt acaggaaatt ttagaaaata aagaaatctt tggtttagat ttatatcaag
     1621 ttggggtagg aaagaaaact gaaaaaatct tttgcgaaat gctagtaggt cctcaagcag
     1681 ttcgtaaaac aatcaataaa tatataacag aagttggagg aaagtaacta tggaaatgac
     1741 ttttagatgg tatggggcaa atgaagaaaa aatctcacta caagaaattc gtcagatccc
     1801 agcaatgaaa ggaattgttg gaacattaat ggatatacca gccggagagg tttggccgag
     1861 agaacgaatt aaatctttaa aagatgaagt cgaagccaat ggacttacct tgaaagttat
     1921 tgagagtgtt aatgttcatg atgatatcaa aattggttta ccaacacgag atcaatacat
     1981 tgagaattat aaacagacga ttcgcaattt ggcagaattt gggattgagg ttatctgtta
     2041 taattttatg ccagtatttg attgggtaaa atcggatttg gattatcgtt tagatgatgg
     2101 gtcttctacg ttagcctata tttcaaaaaa tatccctact gatccggagg aaattattag
     2161 agaagtagaa aattctacta ataattttga attgccgggt tgggaacctc atcggttatc
     2221 acaaattaag caattattag aagcctataa agaagttgat gaagagaaat tacgagagaa
     2281 ctttgcatat tttctcaaag caattatccc gacatgtgaa gaagttggga ttaaaatggc
     2341 agttcaccca gatgatccac catattccat tttcggatta cctcgtattg ttaaaaatcg
     2401 tgacgattta gattggttgt gtaatgtagt ggattctcct tctaatggta ttacattatg
     2461 tactggaagt attgcagaac gaccagataa tgatgtctat gaaattctat ctgaattttg
     2521 tcaacgggat cggattcctt ttgcacatgt tcgtaatatc aaatatttaa gtgacaatgg
     2581 actcgatttt tacgaagcgc cgcataaatc agaatatggt tctctagata tgtataagat
     2641 attaaaaaca atgtacgata atggatttaa tggctatatt cgtccagatc atggtcggat
     2701 gatttgggga gaaaccggta gacctggtta tggattatat gaccgagctc taggtgcagt
     2761 ttatttaaat ggtatttggg aagcgcttga aaagggggcg agtaaataat tatataaggg
     2821 agtttattta gaggaaaaat tttctcttct aaaaactaac ggaaggaaag gagtgtttaa
     2881 atatgttttt agataagaac tttttattag agaatgaaat agctaaaaga ctttttcata
     2941 aatttgcaaa acaccaacca attatcgact atcattgtca tttagaccca gcatatttgc
     3001 tagaaaataa gcaatacaag aatttgactc aggtatggtt aaatgataaa ggagctggag
     3061 atcattataa atggcgattg atgcgagcta atggtataga agaagagttg atcagtggtc
     3121 caggtgatga ttatgaaaaa tttttagctt ttgtaaaaac agtggagaag gcgccaggta
     3181 atcctatttt tgaatggtct catttggaat tacaacgtta ctttgatatt gatttgacaa
     3241 tttctgagaa gaatgcacca gaaatttggc gacgtgctaa cgaaaaaatt caaacggatt
     3301 cctatcgccc gaaagaattg ttggagatga tggatgtgaa agccatctgt acaacggatg
     3361 atcctacaga tgacttgtgg tatcatactg agctttctaa acaggataat tctttcaagg
     3421 tattacctac tttccgtcca gataaggtct ggagtatcaa atcatcaggc ttttcgaact
     3481 atattttaaa acttagtcaa acagcggcta taccaatcaa agactttaat gggttgaagc
     3541 aagcattgag ccaacgagtg gatcattttc atcaaattgg ctgtcgatta gcagatcacg
     3601 gtttgaatac ttatttttat gaaatagcag atgaaaaaat actcgacaat ctgttaataa
     3661 aagctttagc aggaaaaacg gatttccatt tatcagaaat tgcacaattt gttacaggta
     3721 ttcaattaca tttgatgtct gagtatacaa gatatggttg gacgatgcaa atgcatttaa
     3781 atgctttaag aaataattca agtatattaa aggaacaaat tggagtagat gtcggtggtg
     3841 attcgatggg ggatcaagct agtttgacac aaaatgttgt agcattgttc gataccgctg
     3901 aaaagagtgg gtgcttacca aaaacaattt tatattcatt aaatccaact gattggcttc
     3961 cactagcaac agcaatgcaa agcttccaag ggggtatgat tcaaaaaatc caattgggat
     4021 gtgcttggtg gttcaatgat acagctgaag gaatgcgtca acagttgact acgatggcac
     4081 aacaaagttt gttgtcgaat tttacaggta tgttgactga ttctcgaagt tttctaagct
     4141 atcctcgcca tgagtatttt cgtcgagtat tatgtcaatt actaagcgag tgggttaaag
     4201 caggccgaat tccagatgat gaaatgtatc ttagtcagat agtggagaat atttgttatc
     4261 gtaatgcgta taattatttt aatttttttg aaatcggaag tagaaaataa tacttgaaat
     4321 aaaagcttaa atttattctt tgtaagttag tacagctaga caaagtatgt atccataaag
     4381 gaaggagata gatatgaaaa aagtagaaat tttaagtaag ttagaagcgg ctggagtcat
     4441 tgcggttgta cgtgggaaaa ataaggatga ggccattaaa gcaataaatt caattattgc
     4501 gggtggaatt aaagggattg aactaacttt tacagttcct aatgctgaag aaataatcaa
     4561 agaaattgct aaaaaatatg ctgatcaaaa agatgtaata atcggagccg gtacggtttt
     4621 agatgcgttt actgctcgta tcgcaattat ggctggggca gaatatatcg ttagtccatc
     4681 ttttgatcga caaacagctg agatttgtaa tttatatcaa attccttatt taccaggttg
     4741 tatgacaatt actgagataa aggaggcact tactgctggc gtagatatca ttaaactatt
     4801 cccaggttct gcttttggtc catccattgt aaaagcctat caagctccat taccacacgt
     4861 aaatataatg cctactggcg gagtaagtat agagaactta gaagattggt ttgaagtagg
     4921 agttgttgca gttggtgtag gtgggaattt attggcttct gttgaaaaag gtgattttga
     4981 agaagtaagt cgagtagcta gtaaatatat agataagttt tatgagataa agaattatta
     5041 ggagaaaaaa caaataatac tctgaaatgt aaaaaataaa tactgactct ttacgcttca
     5101 gagtatagta cgtactttca tcagaatgaa aacgcttaac aacagaaggg agagaattgt
     5161 atggaaagaa aatttggtat aagagacaaa ataggatata tgtttggaga ttttggaaac
     5221 gattttactt tcatttttgc tagttctttt ttaatgatct tttatacaaa agtattgggg
     5281 ataagcggag ctgccgttgg aacattattt ttagtagcaa gatttatcga tgcagttact
     5341 gatgtaacta tggggcgtat tgttgataga tcgaaagcaa ctaagcaggg taagtttaaa
     5401 ccgtggattt taagaatgag tggtccagtc gctttagcaa gttttttaat gtatcaaact
     5461 tctatgattt cagcttctat gagcgttaag gtagtataca tgtatgttac gtatatatta
     5521 tgggggagta ttttttacac atctataaat attccttatg gatcaatggc atcagctatt
     5581 acttctatta cagaagaaag aacaatgcta tcgacattcc gaaatgttgg agctacttta
     5641 gcaagtttgg tcattggtgt attaacacca ttatttattt atacaagaga tgattcaggc
     5701 gctcaaatag ttcgtggtgg atcaactttt acaattgttg cgggaatatt ttcgatatgt
     5761 gcacttattt gttatttaat atgttataaa ctaactactg aacgagtaag aattgaccaa
     5821 tctcaataca aagtttcatt gttaaaatct ttaaaagggt tagttaaaaa tagggcatta
     5881 ctttctataa ttggtgcggc tattttctta ctccttgctc aattattaat tatgagtatg
     5941 aataattatg tatttcctga ttactataat gatgcacagg gaatagtttt aatgaatttt
     6001 atcaatccta ttttggtttt aatagttgtt tcgcctttaa ctttgttttt aagtaaaaaa
     6061 tatggaaaaa aagaactggc atcagtagga atgttctttt cagcaatagt atatttgatc
     6121 ttatttttga taaaagctag taatatgtat gtattcttaa ttcttagttc ggttggctat
     6181 atgggtttgg gtgtgtttaa tactgttatt tgggcaaata ttactgatgt tattgatgat
     6241 caagaagtaa agtctgatca aagagaagat gggaccgtat atgcggtata ttcttttgct
     6301 agaaaaatag gtcaagctct agctggaggc gtcggaggtt ggactctttc tattataggc
     6361 tatgactctt tagcaaatgt acaaacggga gatgttttag aaaagttata taacgcttca
     6421 acattaattc catcaatttg ttttctgatt gtaggattaa ttttattttt cctttatcca
     6481 ttatctaaag ataaagtctt agaaaattgc gagcttttaa aacaaaaaca taaataggag
     6541 agaaatatga ataattggat attttgtatt gattcagatg gctgtgttat ggatacgatg
     6601 acttataaac acaatctctt tttcggacca attgctgcag agatttttga tgttcatcat
     6661 agagaaaaat tcctacagga ttggaataat attaatttat attcagagac tcgtggaata
     6721 aacagatttt taggtttagt taaaacatta gaaaaaaatg atgtaacatc gattaatcga
     6781 ttaaagcact gggtatctct aacaaaagaa ttgtctaatc catctttgaa gagggaaata
     6841 gaatattctc agtcagagga tttattaaaa gctttaaaat ggtcggaaga agtcaatcaa
     6901 cagattagtc aagcaaaagg attagataaa ccctttttaa gtgctagaga tactataaaa
     6961 gctctgaaaa aatatgggaa gataattatt gtaagctcag ctaataagga agctgttcaa
     7021 gaagaatggg aaagacatga attattatca ttagttgatg aactgtgttg tcaagataaa
     7081 ggaaaaaaag aagatattat tcgaagtgta attgaaaatg gttgtgattt ggataagata
     7141 ttaatgattg gtgattctcc tggtgatcta gaagctgcaa ataaaaataa agttttcttt
     7201 tatcctattt tggtcaataa agaaaaagaa tcatgggaaa acttaagaac tgatgtttta
     7261 tttaa
//
LOCUS       DAZKCY010000121         6759 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000121 DAZKCY010000000
VERSION     DAZKCY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.122"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(179..475)
                     /locus_tag="ACG8XC_002341"
     CDS             complement(179..475)
                     /locus_tag="ACG8XC_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFP9192012.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            830..1186
                     /locus_tag="ACG8XC_002342"
     CDS             830..1186
                     /locus_tag="ACG8XC_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9192013.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1196..2095
                     /locus_tag="ACG8XC_002343"
     CDS             1196..2095
                     /locus_tag="ACG8XC_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9192014.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2615..3343
                     /locus_tag="ACG8XC_002344"
     CDS             2615..3343
                     /locus_tag="ACG8XC_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9192015.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            3488..5515
                     /locus_tag="ACG8XC_002345"
     CDS             3488..5515
                     /locus_tag="ACG8XC_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFP9192016.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            5623..6720
                     /locus_tag="ACG8XC_002346"
     CDS             5623..6720
                     /locus_tag="ACG8XC_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9192017.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         2313 a         1116 c         1357 g         1973 t
ORIGIN      
        1 atgttccatc tgctgcttct cggcaataag cccactagac tagaaaatat gagaagctat
       61 tttctgtcca gcgttcttat tactcaaagc aaatcgcagt tgtcacaatc tcttctaaac
      121 aatgttcaat cagctgacct tcggtattag ctcaaagcac tgatttcaca acctctattt
      181 aggcatgtgt ctcagcaagc ggttgatagt tgatgatctg catttttcga taaaaatcgg
      241 cgggaagctt actccaccgt tttaataatt gatagaaata actctcttct gctttgattt
      301 tatactcgct taatatcaat ccaaaactat tcgttttttt tgaagctaat tttttataaa
      361 tatccaatgc tttggtttta tcttcattga caaagagtgt ttttacttca atataagaac
      421 cctcgatatt ttgtgtttct tcgataaaat agatgttttt tatatattcc ttcatagtga
      481 ttcctccaaa gaagtctttt atttgataag tttactctac cgaagagttg ttaataatcc
      541 gtgaacaaaa agtaattctt tttccaatag ttgttaaagg ttttttattt tttatagtat
      601 tttttgttat ttaagaaagg aataaggtta aacaaataac aggttgcttt ttgggtcttt
      661 ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc tcttttgtga taagagaaat
      721 cataataaac gaatgactta ctttccaata aattgagaac agtcagaacc agacaaatta
      781 gttgcatctg atgaataaag tgattattct atgacatgag gtgatacaga tgaaattaga
      841 agtgacaaaa gaagcacaag aagtattgaa gaataaatta gaagcagacg atcaactttt
      901 attagatatc gaaaacggtg acggtccatt tgcagaaagc caagtcagtt gccaattgga
      961 tacggcattt cgattgatca ttgtcaaaaa agatacacaa acagatctaa gtctttattc
     1021 tgtagtggct gatacaccag ttggaccaat caaaatcaaa gacagtgcaa tactttacat
     1081 ggatgatcca tccacacttg ctttagaacc aacttataat agtttacaat taaaaggacc
     1141 aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa gactagaggg ataaaatggg
     1201 aaaattggcg atcatcagcg acctacatgt ggacatcaat aaattcggcg aatcagagct
     1261 gatgcagctt tggcaagttt tgcaaaagca caagatcact cggcttcatc tagccggaga
     1321 tacggctaat aaagtagacc gttgcctagc tgttgtcgaa tttttcgtca ataaaggatt
     1381 atctacgaca tttcatttcg gcaatcacga gttggcagat gtgcccaatg aacagatgat
     1441 ggaccatttt ccagacccac attttttaaa tgaacggtat attgctttaa atgagcagac
     1501 cgttctttta gggatgaatg gctggtacga ctatcagttt tccgaattga aagattacga
     1561 ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg ttcatccagc gtgggaaaac
     1621 agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa aatttgctag accagctgga
     1681 agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt cctaaacggg catttatcat
     1741 gtatcaatct gctccttatg agcgttggaa caaattaaat gcatttttag gatcagaatc
     1801 attcggagaa ttacttgacc gttattcaaa tatcaaacaa gtagtttttg gccatacgca
     1861 tcgtcgcttt gaagataaat ccatcggtca cacgatttac agctgcagac cttttggtta
     1921 ttattatgaa tggcagctga cccgagattt tgtcttgaaa gaacagttgc tagacagcta
     1981 tgatcctatg aaattacgtg tcctcttgcg tgcccatcac ccagcttttc aggcctatca
     2041 aacacaacat ttgcaagagg aatttgaaca agcgatgaca atcgttccgt attgagacag
     2101 cattagtaat gtgtaaaaga aaataccttg taaaacataa ggataagtaa gagcagtaga
     2161 tccatttatc gtggatatgc tgcttttttt gcggaaaata atcagcgaat gtttatttga
     2221 tcggatgatt tgtaactcgc agagtactag atgcaagcgt gtattctttt tttgaacgat
     2281 aatcacaaaa aaaaatataa tatacatttt ttatttaatt ataattaata ttgattgaag
     2341 tctaggcagt caagtcacat tttgatagat aggaggggga cttacgaatt attttaaagg
     2401 ctatgacagc tgtatttcta gattgtgttt gcatcaagtt gcaataaaaa ctagaaatca
     2461 aatcgggctg ataatcattt ataactaatc gatacatagg accaaatata ggtctatgtt
     2521 cttttatttc ataagtatcg taaaacagaa ttttgctatg gattaatcca atcgactgat
     2581 atgtttagta aataaagaaa gaggaatgtc aatgatgaaa aaagaaatcg tattaacagc
     2641 agcaatgatg ttattcagca tggtagcaag tacgactttt gtttctgcaa ctgaagtata
     2701 cccaaaggaa tataacacag aaggaacgat tacatttgaa gcaggagatg agggtgtaac
     2761 tcctcctgtt gatccagaga atcctgatcc caataaacct gtcgatccat cagatcctcc
     2821 aagccctgga acaggagggg cattatcgat tgattatgga tcaaaattca aatttgggac
     2881 tcaaaaaatt tctactgcgg ataaaacgta ttatgcagct gctgatgtga tgaacgatgg
     2941 gtctagaaaa ccgacatatg tccaagtgac agataggaga agtaccttat ctgggtggaa
     3001 attaagtgtc tctcaaccag aacagtttaa aactgccagt ggggatgaac tagttggagc
     3061 acagctgaaa tttacgaaag ggcaggctgt ttctttggtt gatccaacat atacgccgca
     3121 aacggtgaat tctgaattaa cgttgacacc gggaggaaac aatacgttag caattaatgc
     3181 taaatcagga acaggtgtgg ggacttgggt ctatcgattt ggtgcaaatg aaaatgaaaa
     3241 tcaagatgcg gtacagcttt cggtacccgg taaatctgtc aagttagcgc aacaatattc
     3301 cactaaatta gtttggacat tagaagatac acctaacaac tagtttactg aaaagaactg
     3361 ttaaaaataa atacgtagtc atattgtctt gcgaggtaat cgagcaaaaa ttgtcaaagg
     3421 ctaggctaaa tttgtttgga tacgaatagt tcatctagga atttaacgat caggaggaaa
     3481 agatatattg aaaaagagga ggatcgtgct cattttaagc agtctcttag tgactaatat
     3541 ctttccgatc atagggtact ctttggaatt gaatcagttg gagcatggaa atagaagaga
     3601 cgaagaaagc aaactcactt cgacattcga cagtttaata gaatccaacc agacacaaga
     3661 aagcagtgag gatttaagta tttcgagtag tggagagacc actgacaacg tagaagctga
     3721 gggttcggac aaacaaggac tagaagatgc accaatttct gcggttgagc tgcctgacga
     3781 tcccaatatt atttctatcg ataaaatttt tcaaacacct atcggtacta gcacgtccat
     3841 attggaaaat gggaagttac tgcagttgaa cccagcagaa aggtctcaaa gaggagctat
     3901 ttggtcaaat aagaagatta gtttactctc ggattttaca tttaaatctt atttatactt
     3961 aggaaatcaa cgagatagag cgggagatgg catgaccttt acattgactg gcgatcctag
     4021 aatggctagt aatcccagtc aagttattgg atcgccaggt atgggaattg gctcatattc
     4081 tactcgttct ggccaacctt acattgcgaa tgcgctctcg attgagtttg atacgtataa
     4141 aaatagtggt tcttctgatc gaatggatcg agagataact gctgataata gagggcatgg
     4201 acatgtcgct tttgtcacgc ctaaaccaaa taataataat tattctggag agcatactgg
     4261 cgtgacggta gctcctacat ttctttctaa tggcacatgg cgcttgttaa taattaaatg
     4321 ggacgcggag caaagaagtc ttacttatga tttggaaggt gtcggtcact cttctcggat
     4381 tatacctgat ctcattaggc agtttggttc tacagaagta aattgggggt ttacttcttc
     4441 aacaggtact ttttttcaag aaaatgctgt cgctgtcacg cgacttccat cgaatgtcac
     4501 atcaacagct gatgtaaaag tcaacgatag tgattatggg gccatcgctg aagctggagg
     4561 aaacgataag ataactatca gaaatacatt gaaggtcgag ggggattttc ttgaatataa
     4621 gaaaatgcag actagtatcg acctcccaca agaattaact attcccgagc atatcttgat
     4681 caatggaaat caaattgatg ccaaagatat cttgataacc ggaaatcgag tcacgctaaa
     4741 tctgacagat tattttattg ctggagatac gttaataatc gaattggaga caactttgca
     4801 ggaagtaatt cctgaaaaag tactcactat gcattttgaa taccttgaaa acgggaattt
     4861 gattgagcaa tcgaatgaaa taaacctaaa gatcgcaaag ctgaaggaga tagatatcca
     4921 tgtttattat aaagataaag acagtcagat tgatattgct gaaatgaaaa caatcactgg
     4981 gaaaatagga gagaagtata aagaaactcc tattgatatt gaagggtatg tttttgactc
     5041 ggattcagcg aatacagaag gcatcttctc cgaaaataca gaggatatct atttttatta
     5101 cagaaaaggc aaactttatc tctcaactgc tcctcaatat gtcaattttg gaaaacataa
     5161 aatatccagt agaccattaa gtgaattcgg acagttttca ggtgggttga aaattataga
     5221 tgaacgggct tttggaagtt ggaaattaca attgaaacaa gagaaagttc ttacaaatgg
     5281 agattttgag cttccagaag ctctttcctt tgttacctct gaaaatacaa cggttatagg
     5341 gacatccgct gttacgattt tcgaaagtga tcaaaagggt gaaagcgatt tgtcctctct
     5401 tttagattct acagctcatc gtggaatcag aatagatatt cctgtagaaa accaaagaat
     5461 cggaaccttt gaggggaaac tatcttggat acttgcagat gtcccaggaa attaacagga
     5521 agagaagaat ataacaaaat tattgagttt caagaagggc acaggtacca taggctcttg
     5581 tgtcccacag agacaagatt ttgttatagt cttctcttac atatgattaa aggagcgatt
     5641 ttcttgagac caaaacaaca attatttata acactcctag ttattttagg ggcacttttt
     5701 aacaatatat cagtgtcagc tagtgcattt aatttttcag tgacgccgat aacttcagag
     5761 aatcaaatcg acaagagaaa aacttacttc gatcttcaac ttgatccgga tcaggaagta
     5821 gaagtaaaag cggaattgag aaatgataca gaaaaagaag tgaaaatcga tatttctgtg
     5881 aatagcgcaa ccaccaattc aaatgtgatg gttgaatacg gaaaaaacga gattgaaaaa
     5941 gacatgagcc tgatctttga tctggtagat tatgtgagct atccacagat agtgacgcta
     6001 aaacctaaaa gtgtccaaac cgttgctttt catgtcagga tgccgaatga gcgatttgat
     6061 ggtgtattgg ctggtgggat cacattccaa gaaatccaga cggaaaagga ccaaacagaa
     6121 actaaggatc aaagcttatc gattgagaat gaatacacct atattattgc tttgttgatg
     6181 caacagacac tgaacgaagt tgctccgaac cttactcttc atgaagttaa accagatcaa
     6241 atcaatgcaa gaaatgtcat cttagcaaac gttcaaaatg atcaaaaaac ctatatcaat
     6301 caagtagtta ttgaaacaaa aattacaaaa aaaggacatt ctgaagtttt atatcaagag
     6361 gaaaaagaag gattgcagat tgcgccgaat acaaattttt cttttccaac tgctttaaat
     6421 ggtcaaccgc taaccccagg tgaatatcac cttacaatga ctgttttagg aaatgaaaat
     6481 gaaaatggaa aattttcgag aaaaaaagga aatactacga tcaactatac caatcaatgg
     6541 gtatttgaaa aagattttac aatagacgga aaagtagcca aggaattgaa cacgacggat
     6601 gttacgatta aaaaagacaa taattggatt tatatcttag tagcttcact tttcttattg
     6661 tttattttat tatgcatact tttgtttgtt ttgagaaaaa aagaagagaa agaaaagtaa
     6721 ggttaagata acaaatagta aatttttatt attgtagga
//
LOCUS       DAZKCY010000122         6751 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000122 DAZKCY010000000
VERSION     DAZKCY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.123"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..951
                     /locus_tag="ACG8XC_002347"
     CDS             61..951
                     /locus_tag="ACG8XC_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFP9192018.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1024..1848)
                     /gene="nadE"
                     /locus_tag="ACG8XC_002348"
     CDS             complement(1024..1848)
                     /gene="nadE"
                     /locus_tag="ACG8XC_002348"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP9192019.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1860..3332)
                     /locus_tag="ACG8XC_002349"
     CDS             complement(1860..3332)
                     /locus_tag="ACG8XC_002349"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP9192020.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3969..4331)
                     /locus_tag="ACG8XC_002350"
     CDS             complement(3969..4331)
                     /locus_tag="ACG8XC_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFP9192021.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4554..5306
                     /locus_tag="ACG8XC_002351"
     CDS             4554..5306
                     /locus_tag="ACG8XC_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9192022.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5446..6369)
                     /locus_tag="ACG8XC_002352"
     CDS             complement(5446..6369)
                     /locus_tag="ACG8XC_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9192023.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6384..6656)
                     /locus_tag="ACG8XC_002353"
     CDS             complement(6384..6656)
                     /locus_tag="ACG8XC_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192024.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2064 a         1347 c         1155 g         2185 t
ORIGIN      
        1 tataactttt attaacaaat atgttaatat ataagaaaaa ccttttaaga ggtgatatat
       61 atgcaactac aaataaacga agaatcctta ccggtatatg aagcattagc tagtaaaaca
      121 aggatcaaga tcatccagct actttccaaa aaaaagatga atgtcaaaga tttggcaaaa
      181 gaacttggag tgagcagtgc tatcacgacg atgcacgtga aaaagctaga ggaagcaaac
      241 atcatcaaaa cagaaaaagt aggacaacaa aaaatttcga gccttcgtgt cgataaaatc
      301 gatattagtt ttccagaaaa aatattcaat gcttttgata cgaaagaaac atcgatacct
      361 attggtcact atacaaatta tgcaattgaa ccaacatgcg gtttagctac gatccatgat
      421 tttatcggga aagtggatga accacgctat tttatggatc ctcgaaggat ggatgcccgc
      481 attctctggt ttaccagcgg atttgtcgag tatcaggcac ctaatttttt aaatacagag
      541 gatacattgg agatgttgga agtttctgtg gaaatttctt ctgaatttcc tttttcaaat
      601 gacaattggc cttcagatat cactttcagc ctaaatggtg tggagctagg aacatggacg
      661 agccctggag attttgctga tatccgcggt aaatatacgc ctgattggta tccagataat
      721 cttaatcaat acggcttgct gaagacgatc cgaattacca aacacttgac gaatatgaat
      781 ggagaaccct tgtcaaacat cacgatcaac gacatcccta aagagcaaga cacttggcat
      841 ttgcgaatcg aagtgaaaga cgatgccaaa catgtggggg gctgtacttt atttggtaaa
      901 ggattcggta attacgatca ggatatcaaa ttaaaagttt actacagctg atatttttct
      961 tttctatttt ataaaacaaa aacagccaat ccctatgaag ggacggctgt ttttgcttta
     1021 ttgttatttc cagaaatcat ccaaaacgga aattggcaaa tgtcgtttat gttgtgtctt
     1081 gttccaccat gcttcgattt tttcttgtgc ttgagcggga acttcttttc cttctagata
     1141 atcatcaatt tcattatatg tgacacctag tgcgacttca tctgcaatca ttggtttccc
     1201 atcttcaaga tctgcagtcg gaatttttgt gtataatttt tccggtgcat tcaatgcttg
     1261 aagcaattgt ttcccttgtc gtttgtccaa acggaataat ggcaaaatgt cggctccacc
     1321 atcaccaaat ttcgtgaaga atccagtaat gttttctgca gcatgatctg tccctaatac
     1381 ggctcctgct ctttctccag ctacggcata ttgcgtaatc atacgttgac gtgctttgat
     1441 atttcctttg ttaaaatcgc tgattcccac acctgcttcg cttaatactt gtacttgtgc
     1501 atcaacagct ggtttgatat ttactcgcag actaacatcg ggctggataa agtcaagagc
     1561 agcttttgca tcttcttcat ctgcttgttc accataagga agacgaacag cgataaattg
     1621 atagttttca tcttttgttt cttcacgcat ttcttccatc gttagttgcg ccaaacggcc
     1681 tgctaaagta gaatcttggc cgccgctgat acctagtaca aaagttttaa gaaacggata
     1741 ttttttcaaa tatgctttca taaagtcaat acttttacgt atttcttctt ttggatcaat
     1801 tgttggtttg acacctaatt cattgataat ttctgtctgc aggctcattt attctcctcc
     1861 tatgcttctg tatgaagttc tgctacgctt tttttcattc gttccatgat agccatttta
     1921 tggttccagc attcagtaga cagatcgact gggtattttt gcggattcaa gtctcgttta
     1981 tactcttccc atagtgaatc tagattttct tttgtatatt ctttgatttc gtttagtgca
     2041 ggtaattcgt aaactctttt tccttcgata aagatatctt gtaaaactgg gcgagctgta
     2101 aagtctcgaa cagttttatt gataaacgta tgcactggat ggaacatgaa gatagcttct
     2161 tcttctcgag ggtcttcatc ccataatgtc acgtagtctc cttcagattt cccatcaaaa
     2221 tttcgagtga tgcgccacac ttgttttttc cctggagtag ttactttttc tgcattgctt
     2281 gataacttga tcgtatccat catttttcct tcatcatctt cgatcgatac tagcttgaat
     2341 actgcaccaa gggctggctg atcataggct gtgatcaatt ttgtcccaac accccataca
     2401 tcaattttag ctttttgcat ctttaaattc aaaatcgttg cttcatccaa gtcattcgaa
     2461 gcatagattt ttgcttccgt aaatccggct gcatctaatt gttcccgaac acgcttagat
     2521 atataggcca tatctccact gtcgatgcgt acgccttgga agttgatttt gtcacctaat
     2581 tctttcgcca cgcgaatcgc acttggcaca cctgatttca acgtatcata agtgtcgact
     2641 aagaacacgc agtctttatg ggttttagcg taggccataa atgcttcata atcatttcca
     2701 taagattgga ctaacgaatg agcatgggtc ccgctagctg gtatcccaaa tatcttccca
     2761 gcacgtacat tgcttgttgc atctgctccg ccgatatatg ccgctctagt tccccagact
     2821 gctgcatcca gttcttgtgc ccgtctagtt ccaaattcca atagtggatc ttctccgatg
     2881 acagatttga tacgagctgc ttttgtcgcg atcaacgttt ggaaattgac catattcaac
     2941 agtgctgttt ctactaattg gcagtgcgct aaaggccctt ccacttggat caatggttca
     3001 tttgcaaaaa ctagttctcc ttcacgagca gaacgaactg ttgctttaaa ttcaaaattt
     3061 tgtaaatact ccaaaaaacc ttcaggatag acttctaatc cacgcaagta agcaatatcg
     3121 gaatcattga acgtcagatt ctctaaataa ttgactaacc gttccaatcc tgcaaaaacc
     3181 gcgtatccgt ggttaaaagg catttctcga aagtaacatt caaaaacagc atgtaaatca
     3241 gctcgaccta attcccagta tgtctgcatc atattgattt gatatagatc cgtatgcagc
     3301 gtcaaactgt cgtctgggta agtattattc atcttgtatt actcctcttg gcaagttttc
     3361 atttcctacc cattataaca aaaaaaatta ataggaaaag aaatcttcca ttttttctga
     3421 aaaagcacat gttttacgga tatttcttgc taaaaatgaa ctccatctgc taaaactatc
     3481 ctttcttatt ggtatttaac ctaaacgaga aagaatactt attttattca atgtatccga
     3541 gacacgaaga aattagcgtt ttctattttt cttacttgat aaaataagta gattttccta
     3601 ctacaatcgt agccgttaag ataccgatga ttgaacaaat ataaccaaat gtctctacaa
     3661 cataatcaat ggcaaaagtt tttgaagcaa taatacgtct ttcctttttt gcttcttatc
     3721 cccaaagaaa cggatataac ttattatgta ttgaattttt ttggaaaggc gaattatgac
     3781 aagatatacc ttgcgttgaa aaaactcgcg gaaaaaatat cacacgtttt taaaaagata
     3841 caaagtaaga aagcagctgt caatagaatg gaaacaaaaa tggcgagaca tcaatcagtc
     3901 tcgccttgtt ttttattgta tacattagat atggagcagg ttaccataaa gatgattatt
     3961 agtcgttatt aaatagaatc cttcgtattt ttttgttcta tcactacaat atctttattt
     4021 ggacgtttga attgtattcc ggcacgttct agctcctgtt tcactcctac atggactttg
     4081 acatcttctc gtgtcatttt taaaacgtga tgaatcgcat agtcgatttc ttgccttgtg
     4141 atttttttct tcgtattgat catcacgact tgctttttat catatgtgtc taccaatagc
     4201 agttgtgttc gatctaacga aaataatttg taataattaa tagccgtatt cccaaaaaag
     4261 aattccacta ctttaggata aaatgtctta atatttaaat tactattcac aggtgtttcg
     4321 attagcttca tctcattcct cttttctata tagtcgattt agatccaatc ttaagttatg
     4381 ttaccatctt tcctagcatt aaggaatcgg ttacaattga acctttaaag acaaaaggat
     4441 ttacctttta tcacttaata gtataacata atttcatgag aaaaaaatac aattttttct
     4501 tgaccatctc gttacgtaaa ggattatgct tacatttgtc aggaggaaaa ataatggagt
     4561 acacaataaa gaaaatgtca gagatatcag gagtcagtcc gagaaccttg cgcttttatg
     4621 atgagattgg actgctaaaa ccagctagga tcaattcttc aggttatcgg atttatggca
     4681 aaaaagaagt cgatcgtctg caacacatct tattttatcg aacaatggca ttcaagttgg
     4741 atgatatcca agaggtctta gacaatccat cttttgacca ccagaaagct ttaatcaagc
     4801 atcagcaaat gttgctggaa aaacgggcac aaatcgatac cctcttgaca actgtccaac
     4861 agacacttga tatgtacgaa ggaggaagaa aaatgagtga caaagaaaaa tttgaagggt
     4921 ttaaacaaca aaagttgaaa gaaaatgaag aatcctatgg acaagagatt cgacaaaaat
     4981 acggaaaaaa aaaggttaca gcatccaatg aaaaatggat gaatatggat aaagaaacgt
     5041 atgaagaaat gcaaactgtt gaaaagcaga tgttatttga tctcactctt tacttaaaag
     5101 aaccagaaga tgaaagctta gcagaccgta tcttccaagc tcataagaag tggttatctt
     5161 acagctggcc tgaatatcag ccaaaagcgc ataaaggact aggaatgatg tacgaagccg
     5221 atgaacggtt cactgcatat tatgacgagc gaagtggtca tggtgcagca aaagctttaa
     5281 atgaaattat ccaaaaaaag gcgtaatctt ttacatgatc caacaaaaaa gtacacgtaa
     5341 aatgacctaa taacaaaaca gcagacaccc aagttagatt tttatgctaa cttttggtgt
     5401 ctgctccatt ctttcacaag ttttttcatc ctactttaaa tatattcatc gttgaataaa
     5461 cgtataaggt attttcactt tatccttttt ttctgtaaga aacaattgaa atgccatttc
     5521 tccgcaagct tccagatggt ggtcgatggt tgggaaacca agaagctcgc tgatcagcaa
     5581 attatcgcgt ccaatcacca ctggtgtctt atcttgcaga tagctttgca agatgcctgc
     5641 tgcaaccata tccgaatttg taaagacacc atcaactcca agctctttaa aaaagaagcc
     5701 tgcttgtttt cctttctctg catctataca atcccaaaaa acaagtgatt catcaaaaga
     5761 tggaaaaagt tgatgacata gctgcaaagt gatttttgag ttataactta gcctgccact
     5821 tcgccccaac gtgatgccca attgctttat accttgctgc ttcaaataag taagaccttc
     5881 tgtaattgat tgttctcgat caataaagac acagcttgcc tgttttccct tgatttcttc
     5941 acaaaaaact agaggtccat atttttgata tgccaataaa tcatctagtg tattggcacg
     6001 agacgtaatg atcaatccat cgaaagcttt agctgcaaat tcttcaagat accttcgctc
     6061 tttttctttc tgatagcccg tcggtaaaag tgtcactttg taatcataat caaacgctgc
     6121 ttcaatgatc ccactgatca actgatcaaa atacggatga ttggtataag gcagaatcac
     6181 tccaatgctt ttcgtttttc cataacttaa ctgtctggca tttgcattag ggatataatc
     6241 gacttctttc atgaccgcaa gtatttcttt ccttttttcc tcatctacat atggatgtcc
     6301 attgattaca cgtgaaatag tagaaactga ataacctgtg agctttgcga tatctcgaat
     6361 gtttgccact gctgcttcgc cacctataca aaaagatttt cttgtaaata gtctaccaca
     6421 gctggaccgc attcaaaaaa ctgatacatt tttccttcat gcgaaaaact gatccgctgc
     6481 ccgccccaat atatatcttt tgtcaaattt tcgatttcat tcattggtat ttcttttact
     6541 atattcctat tcaacccttg aaaactgtac aacgttaata cggatccctt gaactcgaca
     6601 aacgcataaa aataattgtt cgttgtgcca tctgttatct ctacataagt attcattgca
     6661 agccctcctt ttgatacttt gagtatagtg tatgaaacgc gtctcatagc aaggagtttg
     6721 ttttgccaaa gaaaagtttt attcctcttt c
//
LOCUS       DAZKCY010000123         6634 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000123 DAZKCY010000000
VERSION     DAZKCY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.124"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..891
                     /locus_tag="ACG8XC_002354"
     CDS             154..891
                     /locus_tag="ACG8XC_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9192025.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            905..1720
                     /locus_tag="ACG8XC_002355"
     CDS             905..1720
                     /locus_tag="ACG8XC_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9192026.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1733..2380
                     /locus_tag="ACG8XC_002356"
     CDS             1733..2380
                     /locus_tag="ACG8XC_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9192027.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2396..3052
                     /locus_tag="ACG8XC_002357"
     CDS             2396..3052
                     /locus_tag="ACG8XC_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9192028.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3214..4035
                     /gene="racE"
                     /locus_tag="ACG8XC_002358"
     CDS             3214..4035
                     /gene="racE"
                     /locus_tag="ACG8XC_002358"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP9192029.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            4038..5393
                     /gene="rph"
                     /locus_tag="ACG8XC_002359"
     CDS             4038..5393
                     /gene="rph"
                     /locus_tag="ACG8XC_002359"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP9192030.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5394..5912
                     /locus_tag="ACG8XC_002360"
     CDS             5394..5912
                     /locus_tag="ACG8XC_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9192031.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            6019..6519
                     /gene="cbpB"
                     /locus_tag="ACG8XC_002361"
     CDS             6019..6519
                     /gene="cbpB"
                     /locus_tag="ACG8XC_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFP9192032.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2200 a         1105 c         1454 g         1875 t
ORIGIN      
        1 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
       61 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
      121 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
      181 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
      241 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
      301 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
      361 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
      421 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
      481 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
      541 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
      601 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
      661 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
      721 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
      781 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
      841 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
      901 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
      961 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
     1021 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
     1081 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
     1141 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
     1201 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
     1261 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
     1321 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
     1381 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
     1441 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
     1501 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
     1561 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
     1621 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
     1681 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
     1741 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
     1801 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
     1861 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
     1921 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
     1981 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
     2041 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
     2101 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
     2161 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
     2221 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
     2281 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
     2341 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
     2401 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
     2461 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
     2521 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
     2581 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
     2641 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
     2701 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
     2761 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
     2821 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
     2881 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
     2941 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
     3001 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
     3061 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
     3121 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
     3181 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
     3241 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
     3301 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
     3361 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
     3421 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
     3481 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
     3541 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
     3601 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
     3661 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
     3721 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
     3781 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
     3841 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
     3901 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
     3961 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
     4021 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
     4081 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
     4141 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
     4201 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
     4261 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
     4321 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
     4381 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
     4441 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
     4501 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
     4561 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
     4621 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
     4681 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
     4741 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
     4801 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
     4861 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
     4921 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
     4981 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
     5041 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
     5101 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
     5161 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
     5221 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
     5281 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
     5341 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
     5401 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
     5461 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
     5521 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
     5581 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
     5641 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
     5701 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
     5761 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
     5821 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
     5881 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
     5941 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
     6001 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
     6061 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
     6121 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
     6181 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
     6241 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
     6301 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
     6361 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
     6421 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
     6481 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
     6541 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
     6601 gggacaaaag cgtttaactc caagaaataa gaag
//
LOCUS       DAZKCY010000124         6593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000124 DAZKCY010000000
VERSION     DAZKCY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.125"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..543)
                     /locus_tag="ACG8XC_002362"
     CDS             complement(<1..543)
                     /locus_tag="ACG8XC_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192033.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAW"
     gene            complement(643..1152)
                     /locus_tag="ACG8XC_002363"
     CDS             complement(643..1152)
                     /locus_tag="ACG8XC_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP9192034.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1353..2222)
                     /gene="scrK"
                     /locus_tag="ACG8XC_002364"
     CDS             complement(1353..2222)
                     /gene="scrK"
                     /locus_tag="ACG8XC_002364"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFP9192035.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2242..3363)
                     /locus_tag="ACG8XC_002365"
     CDS             complement(2242..3363)
                     /locus_tag="ACG8XC_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFP9192036.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3365..3760)
                     /locus_tag="ACG8XC_002366"
     CDS             complement(3365..3760)
                     /locus_tag="ACG8XC_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9192037.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3779..4576)
                     /locus_tag="ACG8XC_002367"
     CDS             complement(3779..4576)
                     /locus_tag="ACG8XC_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9192038.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4578..5366)
                     /locus_tag="ACG8XC_002368"
     CDS             complement(4578..5366)
                     /locus_tag="ACG8XC_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9192039.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5380..5856)
                     /locus_tag="ACG8XC_002369"
     CDS             complement(5380..5856)
                     /locus_tag="ACG8XC_002369"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9192040.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(5928..>6593)
                     /locus_tag="ACG8XC_002370"
     CDS             complement(5928..>6593)
                     /locus_tag="ACG8XC_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9192041.1"
                     /translation="MIVCSCASGLGTSFKLKKILEDSFPRDTDLVIETCDYYSLVNEN
                     YLSELRKENNILFIVGTLNPHLEDVEFFSIEDMILGMNISKFSNQLNRHFNDNELKVL
                     NNNILKNFSLTNLMEQLTILNPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISC
                     LIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYV
                     KRA"
BASE COUNT         2054 a         1409 c          929 g         2201 t
ORIGIN      
        1 ccaagcccag tctaaataat tttttaaaat ctcatcatat tttgcaaata tttcattgcc
       61 atattccgtc cttaaaatta tattttcagt gccagacaag tgatttatat tgagttgata
      121 aaaaagatct ccatctaaaa aaatatcatt agaagaagat ttccccacag taatgaccat
      181 tccatcagct ctatgtgtta catacaaata attgtatgac ctcatttcac ctaatgaatc
      241 aattatttct tgccctttag ctctaatata ttgatctcta ttacctatct cactcttatt
      301 taacaaaagt tttttcatgt caaatttaat cggctgtttt atttttgtaa attcgcttct
      361 actactaatc caatccgaga ttaatttttc aacatgtccc agagtaaact ctgatttatt
      421 cgctaaatac tttttttgtt gtattaaaga catctttccc cgaagctcaa aattgagcct
      481 acatcgattc tctatctctt caaaaagcat cttatcttca tcattcttat attcatcatt
      541 cataaaacca atccagctcc tcgattaaga taatatttta atctcataca aaaactagct
      601 ctcatcaaaa caaccattgt tttgatttct catgaaattt ctttatactt ttctccgatt
      661 ataaattcct aaccccttga gaaaattacg catatattta tctccgcaag gcttaaaatt
      721 tcgttggcct tcttttctca aaaatgcccc gatttcgcct tttgatggat agatttctgc
      781 ctcagcaaaa acgtctaaaa tatcatcagt ggtcatagac aaggcgattt ttaatttttt
      841 tagcaacaca ttgttgatat atttgatttc cttgcttttt tgcggcgtgt ctactatcgg
      901 cttatctttt tgcggaccac gcgccaagag aatcaggcca tttaggaatg cgtcaaaaac
      961 ttggttgttg atggtttttt catacacatt attttctgca ttatctttgt tcttatcctg
     1021 tactttggtc agtaccgcct gagcttcttc tttagtcagt ggtacaccgc ctagctcaaa
     1081 agctttaatc aaatccgtat ctttcaaatc aatcgcataa cgcaatcgca ataacctatc
     1141 attgttattc atttgttcct ccgaagtatt tagtctttac tttcttacgt actttttgtc
     1201 ttgctaagat ttttaggatc aatgataaaa accgcatcac ttatgataag gcgctgggta
     1261 tcatgagtga atgatattgc tgaatacaaa tagtccgacc acattaagtt gatcggactt
     1321 cataagcatt ttattgacta atactttttt gcttaagaat tgattctaac atcttttgct
     1381 aaagttaagc atcctatagt tcctgcatta tctgctaact taggtgtgac aatgtattct
     1441 tcgatatttg gcgtatcgac atagccgtta attagttcta aaaaacgtct ttgtactttc
     1501 tttttcagat gttcttgttg catgacacca ccaccaaaaa ttataatatc tggtgaaagc
     1561 atcagggtcg tattacaggc acattgtgca atataatccg cctcgatttc ccagaacgga
     1621 tcttctatag aaagatcttg tcccttggtt cccgtacgca tctcgatagc gggtccagat
     1681 gccaaccctt cgagacaatc atgatgaaaa ggacaagtac cagagaaagt atctcctgaa
     1741 tgtctcttaa ctaacgtatg ccccatttct ggatggctaa acccttcaac aaaaatccca
     1801 tcttgtatag ctcctccacc aattccagtt ccaacagtgt aataaacaac actggataaa
     1861 ccttttccat gacctgaaac atactccccg taacaagcag cattcacatc ggttgtccag
     1921 gcaattggaa tttcgaaatg cttattcaac gttccaacaa aatcaaaatt tttccaggca
     1981 gtttttggag tagaggtgat atgtccatag gtctttgatt cacgatggat atcaatcggt
     2041 ccaaatgacc ctactccaat gctttctaat tgttctttat agtgattaaa gaaatcaatt
     2101 accaaactca ttgtttcatt cggtgtagtt gtaggaaaac tcactctttc taaaaccttt
     2161 aaatcatcat tgccaatggc acaaacaaat ttagtcccac ctgcttctat tgagccatac
     2221 atttcttact ccaccgtcct tttatctagg aattcgttct tccctgtcta atagtttttc
     2281 gaaaaactca tgtggttcct tttggtacca ataggcaaca gaagaaatat cgtcttgacg
     2341 ctcataaagc ttgatgtcat catttcctag ggtttggaaa gtaacacgta aattttcttt
     2401 gaaactaatg gggtcgggta aatgccagcg gtacaaccca tgcatcggta aagcatcatc
     2461 gccaaaacca tgaactggat ttggattagg ttttccagca ctaaaataat cccgggtgtg
     2521 atctctttta gattgaaaag gatagcccat aaatagcgta ttaaaatttt gagcatatgc
     2581 cctttctcca aagttctttt tatggaaagc ccaagcgccg ccaaaataat cttctgctcc
     2641 tgtagatgtt attgtaggga aatcttcatc tccatctata taaaacttaa attctccttc
     2701 cccccaccag tatctttcca atgcgcacaa agcaaagtag gttcctacat aatacccagt
     2761 gcctttgatt ccatcaataa gggtgtaatc ttttcctttt gtcgtgatgg gttcacggtt
     2821 ccatttagca tgaaaataca tcgcatcatc cggtacttca gtcaatgcat aattaaatgt
     2881 ataaaaaatg tgcttaataa actcaggatg ttcattcgtt aaagtaatct tagctttttt
     2941 tcgaaatggc atctcaaaat agcaattcat ccctgatgtt ggattcacta cgattggtaa
     3001 ggaattcact tcgcatcttg tcccaaaccc attacaaaaa aaatctccta atggactttc
     3061 tacagaagga gtggattcat catcccaata aatacgcaaa attaaatcac gtaagacgaa
     3121 actcccagcg attgttttgt ctgtcactgt catccaaaaa tgacgtaaaa ttccagggcc
     3181 ttccatttct gctaaagtaa ttgtctctcc agcatttatc ggaacagaag gtgacccttt
     3241 tctcccaatt cctagatgac tcgctgtcat tgccgctttc cctttttcac catttttatt
     3301 ttctggtgaa atagttctac tttcctctcc cttgtggagc catatattat ttaaacaatt
     3361 cattttattc gtcctctatc ctttcttcac ttttttgatt attcatatag atgatttgtt
     3421 gttgtgcctc cgcaataatt gctccaatat ctaaattatc atttcctaac tgcaaagcga
     3481 ttgacattgc caagggaaga ttcattcctg caattacgtg cactctgtca gaaatatagg
     3541 taaaaaactt ttgatttaca cttcctccag ccatgtcagt taaaacaatt atttcgtctg
     3601 tagtatccac taactcttta atttgttcat ctatatcatc aatttcttcg acatatgcac
     3661 ataagctact aatttgatta tgcatacctg ttaaaaactc tagtgtctct ttaaaaccct
     3721 cagctagtat tgaatggcta gcaatgatca atcttctcat aattcaaatc cttttccttt
     3781 acgctaatat tccaaatgca gccataacca tcgcaaaaac aataacgagt agaatcaaaa
     3841 tggtcatact gatttttttc ttactgagta gataatatgc tgcagcagta ataattactg
     3901 gaattaatgt tggtaaaatt ttgtccaaca ttggttggat tttcatacta acttcaccaa
     3961 aagtgaattt aagtggcgtt gaaacactga taactgaagc aattaatgat ccaactactg
     4021 ttagccccaa tactgaagca gcttcagtta aataattaat cttttcaccg tattcagtaa
     4081 caatcttcat tccctgctta taaccaaatt caaagcctct cattcggaaa aagaagatta
     4141 ccatgattaa aatgaaccat aaaaagattc ctacaggatt attttctaaa gccatataag
     4201 ccgcaattga tcccatgatg gttgggatca tcgtcatcaa aagagagtcc ccaataccag
     4261 caattggacc catcgtacca actttaaagt cattaatcgc atcttttgct tctaatcctt
     4321 gcttttcctc cattgcaaca caagcaccta aaataatggc tgccagccaa ggctgtgtat
     4381 tgtaatactg aaagtgatta tttaaagctt cgatgtattc atcatcatct ttgtataatt
     4441 ttcttaaaat aggtgacaat gcgtaagtca cagatgcacc ttgctgtgtt tgataattaa
     4501 aggtacaaac tgacatcaac caacgatagt tcgctttcct aagatccttt ttcgttactt
     4561 gatatttttc gctcatttta ttcatcctcc ccaataaacc caacatcaga agctcccgca
     4621 gaagcagtga tttgtgcatc tttttcagct ctatgataca cattaaatgc taaagcgaat
     4681 cctatgatcg ccgctccaat aataggtacc ttcaaaaaag cggctaagaa aaatccaaca
     4741 agtaaataac taatgtatcg acctgtaggc ataaagcgta acaacatcgc aataccaaca
     4801 gcaggcaaca tttttccggc taatgttagc ccatccataa accaattagg taaaacatcc
     4861 aaaacgtaat taacagcaga ctgtcctaaa gtaacggcaa taaatactgg aattgctgca
     4921 gataaaccaa agaaaattgg ccccaaccac aaaacactat tcattttctt aaattctcgt
     4981 ttattcgcat attcctgtga tttgcgaaca acaaaaccat ttaagatttt gacaattaca
     5041 tctagttgca cccctaacat acctacaggt agtcctattg ctaatccggc ttctactcca
     5101 agctggctac caaatgccgt tgcaataatt gcgcacgttc catagtccgg tacagatgag
     5161 ccaccaaaac cggcaactcc taatgacatc aattgaactg taccgccaac tatcaaacct
     5221 gtttttatat cacccatcaa aattcctgtg aataatcccc aaaaaactgc gtaatttaca
     5281 aagatcatcg gaatcccata ataatcaaca tgtttaataa atgctaatat agtcagcata
     5341 ataatttgaa aaatagtaaa atgcattatc cttactcctt tatcctaatt ttgattgaat
     5401 ttcctgtgcc ttatccgccg gaacaaattg tgtaacaatt tctacaccag aatccaacaa
     5461 ttttttatac gtgctgatat tttcatctga tgcataggct ctagaagata gctgtaatgc
     5521 attatcttta acaacagtac cgccaacaat tagtcggtca attctaatac cgttatctac
     5581 taaatgaagg attgtttccg gctctttcac gatgacaaat actttttcct gatcgtattt
     5641 ccccttttta aaattcgcaa tcgctgtttc ttcagtgata attgatacag ccattccagc
     5701 aggtctagct aaacgcatac ttgatttttg gatgtcgtta tttgcgacag aatcatcgat
     5761 caccatcact ctgtttgctt tagacattgg tgcccattgc gtgacaataa tcccatgcaa
     5821 taaacggtta tcaactctcg acataacaat actcataaat acttcctcct ttatctttca
     5881 ttttcttaca agcaattacc atgccaaaac aaaaagaact tgataaatca agctcttttt
     5941 acataatcat aaacgtattt aacctcatga atcgggatgt cgacactata ataatgttca
     6001 acgacactga aagattgctt aaaatgtgtc tgaaattttt gaaattcctc actcgtttca
     6061 ttgaaataaa tttcatcttc caaagtattt tgttttacta atcgttcaat taagcaacta
     6121 atgtgaacat ataatccgaa acacgtatta ttatcaaatg aaattcctag atcattttgt
     6181 aagatgtata tagcatctga aacccgttct aataatttag tcggatttaa tatggtcaat
     6241 tgctccatta aattcgttag actaaaattc ttcagaatat tattgttcaa aactttcagt
     6301 tcattatcat taaagtgtct attcaattga ttggagaatt tacttatatt cattcctaaa
     6361 atcatatctt caattgaaaa gaattctaca tcttctagat gtggatttag tgttccaaca
     6421 ataaaaagaa tattgttctc tttacgaagc tccgataaat agttttcatt cactaaagag
     6481 taatagtcac acgtttctat gacaagatca gtatcccttg gaaagctatc ttcaagtatc
     6541 ttttttaatt taaacgaagt tcctaatccg cttgcacaag aacagacaat cat
//
LOCUS       DAZKCY010000125         6570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000125 DAZKCY010000000
VERSION     DAZKCY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.126"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG8XC_002371"
     CDS             127..579
                     /locus_tag="ACG8XC_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192042.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG8XC_002372"
     CDS             593..1282
                     /locus_tag="ACG8XC_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192043.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACG8XC_002373"
     CDS             1407..3758
                     /locus_tag="ACG8XC_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFP9192044.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACG8XC_002374"
     CDS             3838..4233
                     /locus_tag="ACG8XC_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192045.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="ACG8XC_002375"
     CDS             4558..5175
                     /locus_tag="ACG8XC_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9192046.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="ACG8XC_002376"
     CDS             5216..5515
                     /locus_tag="ACG8XC_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFP9192047.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="ACG8XC_002377"
     CDS             5549..5716
                     /locus_tag="ACG8XC_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9192048.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="ACG8XC_002378"
     CDS             complement(5761..6366)
                     /locus_tag="ACG8XC_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9192049.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DAZKCY010000126         6531 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000126 DAZKCY010000000
VERSION     DAZKCY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.127"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..2026
                     /locus_tag="ACG8XC_002379"
     CDS             104..2026
                     /locus_tag="ACG8XC_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9192050.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            2027..2473
                     /locus_tag="ACG8XC_002380"
     CDS             2027..2473
                     /locus_tag="ACG8XC_002380"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFP9192051.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            2489..2803
                     /locus_tag="ACG8XC_002381"
     CDS             2489..2803
                     /locus_tag="ACG8XC_002381"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP9192052.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            2829..3878
                     /locus_tag="ACG8XC_002382"
     CDS             2829..3878
                     /locus_tag="ACG8XC_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9192053.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            3890..6493
                     /locus_tag="ACG8XC_002383"
     CDS             3890..6493
                     /locus_tag="ACG8XC_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP9192054.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
BASE COUNT         2375 a          935 c         1278 g         1943 t
ORIGIN      
        1 taatcaagaa tattagaaac ttctacatta atcaagaact ttcttctgta tgaaaataga
       61 gataataatt gtacacttaa gctgtattca ggaggtgaag ataatgggca tcgaatttag
      121 cgaacggcaa atgaaaatac tatacttttt gattgaaaat gagcacacaa cttacaaaga
      181 attatcagaa caattatatg tatctacgaa aacaattagc aaagatattt taattttgaa
      241 agaaataatc caaaaaatgc cagatgtaca aatgttggtt caacctaaaa aaggcgtttc
      301 gcttattgga aaaaaagaaa cacttcgaaa tttattatcc agtcataaaa tcaatatcgc
      361 agactctccg gaggaccgtc taacttatgt gtttgctaaa ttattgaata ctaatgattc
      421 tctaaagatt caaaatcttg ctgacgaatt atacgtgagc aggtcgacgg tagaaagcac
      481 aatgaaagat gttagaaaaa gactaaacaa gcatgggatg tccttagttt cagatcgaaa
      541 tggcgtgcgc atcaaagctg atgaaaaaag taaaagaagg ttgatgtcag aagttgtgaa
      601 ctattattgg ggtggcttta cagcatctaa cagtaaaaca gatctcttag acttgaatat
      661 caatttcatt ggtgaagcga ataatttctt aaataaggac atattgaaaa aagtagccga
      721 attgcttaat cgatacatga ataagacaca attgaaattt acagattatg aatttcaaac
      781 tttagctatc caccttgcta ttgcgataga aaggattgaa acaagcaatt tcttatcacg
      841 tagtaataat tctgaagaag aaatctcaaa gaatacactt attttaatca aaatgattga
      901 aaaagcattt tcaattattg tgccaataga tgaacaagct tatgttgaca ttcacattac
      961 ttcgattgaa aaaaatgcta tgaatcacga cttagatcaa acgaagcttt cctatttaga
     1021 tatgggtgtt gagctacaag agttagtaag aattaaatta aatgaatttt atcctgatga
     1081 agaattgatc aataatctag tattacatct caatgcagca gttaaaagac tcaaattggg
     1141 agtaaatatc tataaccctt atacggacaa aatcaaatat tcttttaaaa ggtcatttat
     1201 gataagtgta gatttattag aggaaatcga agagagattc tgtatacatt ttaatgaaga
     1261 tgaaatcgct tacgtaacac tccatattca atcacttttg gatcgttaca aacctgacaa
     1321 aacgaaagtg atattagttt gtagtagcgg atatggaaca tcaaaattat tagagcaaag
     1381 aataaataac ggttttgcag atatggttga gatagaagat gtactaagca tcaatgaact
     1441 acaagataga aaagtcactg atgaacttgt tataagcacg attccgattg aaaatacaaa
     1501 ttttcctgtt attgttgtta gtcctttgat gttaggggca gatgtcagaa aagtaaaaca
     1561 gtatctacag ttaaatgaac aaacagaaga tgcttttcta aatttattag ataaacgatt
     1621 ttgctttttt acccaccaaa taaaggataa tcaagtgaac gttttaaata taattaccaa
     1681 aaaattggta aaagaaaatt acgcgaaaga agggatacta gagggagcaa tcaaaagaga
     1741 aaaactatct tcaacagcga tgaatgattt tgctatgccg catatcgaag tagattttat
     1801 aaaaaagcca gtaatctgtg tctacgttaa tccagaagga attgattggg atgggacact
     1861 tgttcattct gttttctttt ttgcacttaa tgaaaaagta aaacctgaat tgaataaaat
     1921 ttatgaaaca ttcaatgaaa tcgcttcgaa tcatgagtta ttagcaaatt taaaaacgag
     1981 ttcgtcgttt gaaaaattag tttatatatt gaaagaaggc ttataaatga tcagtgaaaa
     2041 aatcaatata aataatatag tgatagattt aaatgcatct gataaaaaaa gtgtagtcga
     2101 aaaattatcg aaattgttag agactaatgg gtttgtgtct gaccacaaac aatttcaaaa
     2161 agacgtattt gacagagaaa accatatgac cacaggaatt gggaaaaata tcgccattcc
     2221 tcatggcaaa agcctagcag taattgaatc aacagtggct gtagccaagt taaagcggtc
     2281 tgttgaatgg ggcgcattag atgatcaacc agttgatctc gtctttttgt tagcaataaa
     2341 agacactgat aaaggtacta atcatttacg tattttagca gaattatctg gaagattgat
     2401 ggatgacgag tttgttaaaa gaataaaaga ttcgagaaat aaaaaagaat tggtaaaagc
     2461 tctatcattt taaaaggagg aatttcatat gacaaaaata gtagcagtaa cagcatgtat
     2521 cgcagggatt gcccatactt atatggcaaa agctaattta gaaaagtttg cgaagaagaa
     2581 agggttagat attaaagtag aaacacaggg ggctatgggg atagaaaaca aactttcaat
     2641 ttcggatgtt tccgaggcag atgttgtcat atttgcagta gatacaaatg tttcagaacg
     2701 aggtcgcttt gacggtaaac cgattttaac agtagggacc tcagaggtag taaaagatgg
     2761 tgaagagacg attgatgaag caattgcatt aattaacaaa taaaaaatat atcgtggggg
     2821 agttactaat ggaattcaag aaacaaggaa gagaaattct aaatcatttc caaagtgggg
     2881 tttcctatat gatcccgctt gtcgtagcag cagggttact aacttctatt gcagtcattt
     2941 ttggtggcac aggtgtttgg gaccagacgg atacattctg gggagtcctg cgaatgattg
     3001 gtcagacggg attacagttt atcgtaccga tgatttcagc ctatatcgca tattcaattg
     3061 ctgaccgtcc cggtctagca ccagctttta tcactgggat gatgtgtcaa aatcttggta
     3121 tgggatttat tggaggaatg gttgctggat tagcttgtgg ctatttagct aatgcattga
     3181 aaaaagtaaa agtaccgatg caagttatta gtctaaaatc aattatgctg attccttttg
     3241 ttactacatt agttgttgga atcatattat ggtatgtact agctacgcct attcaagcaa
     3301 tgacaacagg actgactaat tggctagaag gcatgtcagg agcaaatgct gctttgatgt
     3361 cggctatttt aggaggaatg atggcgtttg atatgggagg acctatcaat aaaatcgcat
     3421 atgcttttgg ggttgcttct ttttcaagcg gaagctttgg acctagtaca gccatgttat
     3481 taggaattgc tattccgcca tttggaatgt ttttagcaac aattttaaac aaaaaacttt
     3541 atgatgaatc ggaacgagat aatgcaaaga cagctatgat tatgggcctt gtggggatta
     3601 ctgaagggac gatcccgttt gccgtgaaag atcctttacg agtaatacca agcatcgtgg
     3661 tgggcactgc agtagcatgt ggtctaaatg gattttttgg tgttactcaa gaaacaatgc
     3721 tttcaacctt tatggcaatt ccttttgtta cgaatatacc tttatattta gtatcaattg
     3781 ttattggggg agtggttaca gcgttaatgg tcaatgcact aaaatccatc aagtataaaa
     3841 aagaacaaca agtaaattca gtagaagaag gaaagtagga gaatctgata tggtaaatgc
     3901 acatataatt caacacacgc actgggacag agaatggtat tttacaaatg aagatgccat
     3961 cgtattgtct gatcaggtct ttactgaagt tctagatgaa ttggaaagga accctaaagt
     4021 aaacttttgt ttagatggac aaacttcaat cgtcgatgag tatttggaga tcaatccgca
     4081 aaagcttaag gttatccaac gactgatgaa aacaggacaa ctattcgtgg gaccgtggta
     4141 tgcacaaaca gatgccttat tagtggatgc tgaatcaatt ttaagaaact taatgattgg
     4201 aatcagtgat acgatcctta aatatggaga tccaatgatg gtagggtatt tgcctgacac
     4261 gtttgggttc aatgcgaatt tgcctacact tttgcatcaa gtagaaatcg ataatttcat
     4321 gtgttggcga ggattaaact ttgaaacgat ggttccatct ccttatttta tctggaaagg
     4381 attgggagat aaatttgttt atgccatgaa ttttccattt ggttatatga ctggaatgat
     4441 gaccttagac gcaattaaag atattcctga atttgcaaaa gaaaagttgg atgaagcagt
     4501 tgaaatgctt catatgcaag gaaaaaacaa agatatttta gtaccttcgg gtgtagatca
     4561 aaagtcaatg attttaaatt ttgatcaagt cgtagagaag ttgaataaat acagtaaatt
     4621 ccacaatatt atttcagatt atccttctta tgtagaagtt atcagaaaca aaaaagaatt
     4681 acctgaatat aaaggtgaac ttcgtcagcc ggtttactct cgtgttcatc gttctattgg
     4741 ctcagtaaga acaaaaatga aaatagaaaa ttttaaattg gaacaaaaaa ttctaaaacg
     4801 agttgagccg ctcatgctga ttgcagaaag aaatggtatc catatcagta aaggcctact
     4861 tcgtcggtta tggaaaaaga ttttgcaaaa ccaggctcat gatagtattg gtggctgtgt
     4921 ttcagataat gttgcggaag atattttcca tcgaatcaaa gaagctaatg agatagctga
     4981 cggaatcgaa aaccttattg taaaacggat ggctgatgca ttaaagctaa aatcgaatga
     5041 agtccttgtt ttcaacacaa cgactgagaa ttatagtgga tcgaaaatcg tgcatgtagt
     5101 agctgattca aagaaaatac acttcaaagg tagtccctat aatatcattg aaaaagaagt
     5161 ttattttcca gaacggaaaa atgcttatcg gatgattgct acaggtttcg aatattttga
     5221 tgaaccagct tattatgaac ttgacataaa aataaatgta aatgtgccag cactaggata
     5281 cactgttgtt gaatttgaga aaaataatga aaaattagaa acagcaatcg agatagacaa
     5341 aactgagata agtaacaata aatataaact ttcttttaaa gatgggcaat tgaatttgat
     5401 agttggagat agacaatatc ttgatttcgt tcatttgatc gattcagcaa atgatggcga
     5461 tacgtatgat tattcaccat tagaaggaga tacagaacta tctctaaaat tagaaacagc
     5521 taaagtatat aaagattctt tacaagaaac attagttgtt tacggaaaag ctcaacttcc
     5581 taaagattta aaagatcgtt tatctgaaaa acccgaaatg gaagaaatat cttatgaaat
     5641 atcgttcagt ctgggagaaa gtcaaatcgt tgaaggcaca ctcaaaattc ataatactgt
     5701 ttactcgcat cgtatgcgct tagcaattaa tgttttagat aaatctgaaa aatcaattgc
     5761 acaaattcaa aacggattta ttgaaaatac ccctgatgtc attcctccga attggtcaga
     5821 aactatggta gagaaacctg ttaatttaga aatattcgat aagagtgtct ctgtggcaga
     5881 aaatgatcat tattttacgt tttatgcaga tggtttaaaa gagtatgaac gtatagaaaa
     5941 tcaattaatc ataaccttat tttcaacaac aggggaactt ggaaaaccta atcttgcttg
     6001 gcgacctggg cgagcttcag gagatacgac aaatgaaggg catgtcatga tggaaacacc
     6061 gctagctcaa gagataggtg agtataaagt cacttttgga tttaatgtag aagaaggaag
     6121 atttaatgag ttcaaagtgg caaaacaggc agaaaaacgg ttagaacaat ctatttctta
     6181 tcaaaagcag aaactgaatg tatttattca caggttagat aacaagattt ggccattaca
     6241 aaatgaactt gatgttcctc gtaaattttc attgattgaa ttacctgaag acttaattgt
     6301 atcatcgatt tatccttctt attttcatga acaaggattt gttcttcgct tagctaatcc
     6361 aacagaacaa gaaaaaatag tacctgaggc tatcctttcc cttgggacag ttgtcaatgc
     6421 actggagaat aaacaagaat taacaacaat tcctccctat gattatttgt caattttgat
     6481 aaatgagaga taaatatgta atatacaaga aaaaaacacc ttttattttg a
//
LOCUS       DAZKCY010000127         6416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000127 DAZKCY010000000
VERSION     DAZKCY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.128"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..232)
                     /gene="rpmB"
                     /locus_tag="ACG8XC_002384"
     CDS             complement(44..232)
                     /gene="rpmB"
                     /locus_tag="ACG8XC_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP9192055.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            502..864
                     /locus_tag="ACG8XC_002385"
     CDS             502..864
                     /locus_tag="ACG8XC_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9192056.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            916..2598
                     /locus_tag="ACG8XC_002386"
     CDS             916..2598
                     /locus_tag="ACG8XC_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFP9192057.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2715..4751
                     /gene="recG"
                     /locus_tag="ACG8XC_002387"
     CDS             2715..4751
                     /gene="recG"
                     /locus_tag="ACG8XC_002387"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP9192058.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4800..5801
                     /gene="plsX"
                     /locus_tag="ACG8XC_002388"
     CDS             4800..5801
                     /gene="plsX"
                     /locus_tag="ACG8XC_002388"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP9192059.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5923..6165
                     /gene="acpP"
                     /locus_tag="ACG8XC_002389"
     CDS             5923..6165
                     /gene="acpP"
                     /locus_tag="ACG8XC_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9192060.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2139 a         1108 c         1448 g         1721 t
ORIGIN      
        1 ataaaaaacc gttcactatc tgaacggtcc aagtatcatt caattaaacg cgttcaactt
       61 ttcctgattt caaagcacga gttgacaccc aaactttttt aggttttccg tcgaccataa
      121 cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt tgagttcata gcgtgtgaac
      181 ggttgtttcc actttttgtc ttgcgacctg taaagtaaca aacttttgcc atggtaattt
      241 cctcctttgc cttgatttgg agcaatagaa taattcagct cataccagac taacatatca
      301 tatagaatgg gaatttgcaa gtacattttc atagatacaa ttgacaattg agaagaataa
      361 ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt ttttgagtac tttgggtaac
      421 tgccttttat ttcaagttgc gctatgataa aatatgatag aagaaaaatg ggagtttttt
      481 acccgttaag gaggctcatt catggctgta aaaattcaaa cgtcagctgg aaccattgaa
      541 attaccaatg atgtaattgc aacagttgtt ggtggcgccg cgacagatgt gttcggtgtt
      601 gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta ctgagatttt gcgtaaagaa
      661 aattactcaa gaggagtagt tgtccgccaa gaagataatg gtattgctgt agatgtttat
      721 acgattatca gctatggtac gaaaatctct gaagtttccc gcaacgtcca agaaaaagtg
      781 aaatacaatc tagaggcatt attaggcgta actgccaatt cagttaatgt ctttgtacaa
      841 ggtgttcgtg tactgccaga ctaggcagga gactggctgg taactcagca gtcgatctga
      901 aggaggatta tctaggtgag tgtaacagaa ataagtgcaa gccagttcca ggaaatggtc
      961 caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg tcaattcatt aaatgtattc
     1021 cctgtgccag atggcgatac gggaacaaat atgaatctat ctatgactag tggagctaag
     1081 gcagtagtag attcaacctc agaaaaagta ggagaattag cagcaatgct ttcaaaagga
     1141 ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt ctcaattgtt tcgtggattt
     1201 tctaagcaaa ttccagatgt tgtctcatta aatgcttctg atcttgctgc agccttcact
     1261 catggagttg aaactgcgta taaagcagta atgaaaccag tagaaggaac gattttgaca
     1321 gtagctagag aagcagcgaa agctggagaa aagaaagcaa aaagtactga tgatgtcgtt
     1381 gaagtaatga cagctgttgt aaaaggagca aagcgtgctt tagctaaaac tccagacttg
     1441 cttccagtcc taaaagaagt cggtgtcgta gacagtggag ggcaaggact gctgtttgtt
     1501 tatgagggct ttttaagtgc attgaatgga gaattccaag cggatgaaac atatgaacct
     1561 tctccagcag aaatggatga tatggtcaat gcagaacatc atcgcagtgt ccaaggtcaa
     1621 atggcgacag aagatatcca ttttggttac tgtacagaaa tcatggtaaa aatcggagaa
     1681 ggacctacag tcgatagtac attcgattac gaagaattcc gtaactattt agatggtata
     1741 ggagattctt tacttgtggt caatgatgac gagatcatca aagtccacgt gcatacagaa
     1801 aacccaggag aagtcttgaa ttatggacaa aaattcggtt cattagtcaa aatcaaagtc
     1861 gacaacatgc gcttgcagca tgagacgatt ttggaacacg atgaagaagt gaacgagttt
     1921 gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag cgatcgctgc tggtaaaggc
     1981 gtccaagagc tgtttgaaag tttaggcgct aattatgtta tcagtggtgg acaaacaatg
     2041 aatccaagta cggaagacat tgttaaagcg attcaagaag taaacgcaga taaagtgatt
     2101 gtcttgccaa acaataagaa catcttcatg gcagctgatc aagcagcaga agtagcagat
     2161 atcccagtag cagttgttcc ttcaagaacg atttcccaag gattgacagc tatgctttca
     2221 ttcaatgatc aagcttcttt agaagaaaac aaagaaacga tgacagaaat gttgtctagc
     2281 gttgtgagcg gtcaagtcac tcacgcgatc agagatacct caattgacgg tgtagagatc
     2341 aaagaaggag actttttggg catgatcgat ggaaaaatcc tgatctctaa cccagatatc
     2401 acagcaacag cgttaagtac actagaacaa atgatcaatg aggacacaga gatcgtaacg
     2461 atcttggcag gtgatgaagg aacagttgaa caagcaaatg aatttgctga gaaactaaca
     2521 gaaaaacatc ctgatttgga aatcgagatc caccaaggag atcagcctgt ctatccttat
     2581 ctattttctg ctgaataatt tctgtgtcag aatatctttc taacacagta ttcattcata
     2641 aaaggccttc ggttagtgat caggtatgat cgctgaccaa gtcctttttt tgttaaagga
     2701 gggaggaaaa atttatgcgt tctttagatg atccaatcac tatgctggca ggagtcggag
     2761 aaaaaagagc agctagtctt gccagcttgg gcatccaaac gattgaagac ttactgactt
     2821 attatccttt tcgctacgaa gatattcagg aaagaaatct aaacgagatc caggatcaag
     2881 aaaaggttac tttgaaagga atcgtagtct cgcctcctgt gatgaatcga tttggttata
     2941 aaaaaagccg attgcaattt cgtatgatgc aagaacatga tgtgttcaac gtttcttttt
     3001 ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga ggaaatcgcc gtatacggta
     3061 aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat tttaggttct caaacaatgg
     3121 atgacttttc gccgatatat cacgtcaaca aatcaattcg tcaaacgaca ttagttgatc
     3181 taatcaaaaa agggtttcaa gaatacggca cattgattga agaaaatctg ccaaacaacc
     3241 ttgtggagaa atatcgactg cttgaccgag cttctgctgt aagaagtatg cattttccaa
     3301 aagaccacga agaaaaccat caagccaaac gacgtgtcgt ttttgaagag ttctttttat
     3361 ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc agaaacgaat ggtttggaga
     3421 tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa gctacctttt gaactgacaa
     3481 atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct tcgcagtccg cagcatatgc
     3541 agcgactttt gcagggagat gtaggtagcg gaaaaacagt ggttgccgcg attgcgttat
     3601 acgctaccgt tacagcaggt ttccaaggtg cgttgatggt gcctacagaa atcttggcac
     3661 aacaacatat ggaaagcttg caacagctgt ttgatcccaa tgaagtaacc atcgcattga
     3721 ttacggggtc aacgaaagca aaagaacgac gtgagctcct tgaaaaacta gagcaagggg
     3781 aaattaacgt cgtgatcggc acacatgcat tgatccaaga tggagtcgaa tttcagaatt
     3841 taggtctagt tattacggat gagcagcacc gttttggcgt gaaccagcgg aaagtacttc
     3901 gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc gactccgatt cctagaacat
     3961 tagctattac agcatatggt gagatggatg tatctatcat tgatgaactt ccagctggac
     4021 gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt ggacagtgta ctggattgga
     4081 cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat ctgtccgctg attgaagaat
     4141 cagaagcatt agatgtaaaa aatgcagtgg aaatctatga aaaacttcgt gaactatttg
     4201 caccaaaata tgaagttggc ttgttacatg gtaaaatgaa gaatcaggaa aaagaagtaa
     4261 tcatggaagc cttcaaagac aaccagatgc agatattagt ttcgactact gtaattgaag
     4321 taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat ggatgcggat cgatttggat
     4381 tggcacagct gcaccaactt cgtggacgtg ttggacgcgg aagtgatgct tcctactgta
     4441 tcttagttgc taatcctaaa aatgaactag gtgtggaacg gatgaaaatc atgacagaga
     4501 ccaccaatgg ttttgtacta agcgaaaaag atctggaatt acgtggtcct ggtgaagtat
     4561 ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc tgacttggtg acggatggaa
     4621 atgttttgga ggtagccaga gaagaggctg cagccctttg gcaaataaag gattggcaga
     4681 tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat agaaaatcag gataatcgtt
     4741 atttcgatta gcagactgtg ctatacttta tactgatgac ggaaaaagga gtggaaacaa
     4801 tgaaaattgc cgtagatgca atgggcggag atcacgcacc tcaagcaatt gtagaaggcg
     4861 tggctttagc gcagcaagat tttccagaag tagaattctt actttacgga aaagaagcag
     4921 aaatcaagaa atacttaaca aatgaaaaaa acatcacgat tatccataca gacgaaaaaa
     4981 tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa aaagactgca tcaatggtcc
     5041 ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt attttcagca gggaatacag
     5101 gcgcgctact agcagcaggg ttgttcatcg taggacgtat caaaaatatc gaacgccctg
     5161 gcttgatgtc taccttgcca gtagtaggga aagaaggtgc cggtttcgat atgctggatc
     5221 ttggcgctaa cgcagaaaac aaaccagaac atttgttgca atacggtatt ttaggttctt
     5281 tttatgcacg taaagtccga ggtatcggac gtcctcgtgt cgcactctta aacaacggaa
     5341 cagaagcaac aaaaggtagt gaagtgacga aaaaagccta tgaactcctt cagaatgaaa
     5401 caagcttgaa ttttattgga aacgtagaag ctcgtgagct actcaatggt gtggctgatg
     5461 tagtggtcac agatggattt acaggcaacg ctgttttgaa atctattgaa ggaacagcaa
     5521 tgaatatgat gaatctatta aaatctgcta ttctaaatga agggatcaaa ggaaaaatgg
     5581 gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc agaaatggat tattccaaac
     5641 atggcggtgc cgttttgttt ggcttgaaag caccagtcat taaaactcat ggagcaacag
     5701 gccccgatgc tgttcgctat acgatccgcc aaatccacac aatgcttgag acagatgtag
     5761 ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata gtatttggat atctgaagcg
     5821 atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat gccagtaaaa ttagccatgg
     5881 tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa agttgactcg cgaagaagta
     5941 tttaacaaag tagcgaaaat tatttcaaat cattttgaga tagataccga taaagtgacg
     6001 gatgaattga acatcaaaga tgatttaaaa gctgattcaa ttagtattat ggaattcgtt
     6061 ttagagcttg aagatgaatt cggtacggaa atttcagatg aagacgctga acaaattgaa
     6121 acagtcggtg gcgcagtaga ttatatcagc agccatctta aataatttca aataaaagca
     6181 aagaaggata aaaagctgca tgcagctttt tatccttctt tttttaatct ttttgacacg
     6241 taaaagtcat ttttctgata attctggcga taaacagaaa ttatgttgca aagtaaagcg
     6301 gttttctata taattattaa taattcatta cacaaagata agaataacct ttgttataaa
     6361 acgatttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta ggaagc
//
LOCUS       DAZKCY010000128         6369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000128 DAZKCY010000000
VERSION     DAZKCY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.129"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2560
                     /locus_tag="ACG8XC_002390"
     CDS             <1..2560
                     /locus_tag="ACG8XC_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFP9192061.1"
                     /translation="FSTADSQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRD
                     NDIEKIRFVFIDIDPKRKEGSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGY
                     HIFLPINEQPNNHETILTIQNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVK
                     GKSTKERPHRLSSFLSIPDNIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFI
                     QADYKQWLAHYPELTYTIKEGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRF
                     HCLHASDKNKDIQDFVKKYPLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQE
                     EKVACPMFIKQIRKNIEQKVITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLII
                     KARREAEVVDFLQIQRENAPIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSC
                     YQLTTNGTEAEFDALVHKYVLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQG
                     KSTTGKTTTLHFIASLFGNPTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQN
                     NFSSLIYQLASGEERLRLDSNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYI
                     EFSDIKFTKDANHAEKVKSLSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAV
                     LPEHSLKQRIGNNLALIYSGALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQ
                     TVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRRE
                     FQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2742..2981
                     /locus_tag="ACG8XC_002391"
     CDS             2742..2981
                     /locus_tag="ACG8XC_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192062.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            2997..4370
                     /locus_tag="ACG8XC_002392"
     CDS             2997..4370
                     /locus_tag="ACG8XC_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9192063.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4374..6008
                     /locus_tag="ACG8XC_002393"
     CDS             4374..6008
                     /locus_tag="ACG8XC_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9192064.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6171..>6369
                     /locus_tag="ACG8XC_002394"
     CDS             6171..>6369
                     /locus_tag="ACG8XC_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192065.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2229 a         1041 c         1287 g         1812 t
ORIGIN      
        1 tttttctact gcagattctc aagcaattat taactattta gaaaaacaga aattgtctga
       61 aggtacagta aacataactt ataatcctat taaacaagag gtgattacac aaaaaaagca
      121 ttctgtgaga gacaatgata ttgaaaaaat tcggtttgta tttattgata ttgaccctaa
      181 aaggaaagag ggaagtgcaa cagatagcga aaagaaaaag gcagaaaatg ttatggaaca
      241 agtagaacgt tatttgaaag aaaagagaat agaatcattt gtaaaagttg atagtgggaa
      301 tggctaccat atttttcttc ccataaatga acaaccaaat aatcacgaga cgattcttac
      361 tattcagaat tttcttcaat tacttcaccg aagatttgga gtagaagatg aagttgatat
      421 tgatacttct gtttataatc cttcaagatt atgcaaatta gttggtacac cagcggtcaa
      481 aggaaagtcc acaaaagagc gtccgcatcg tttatctagt ttcctttcta ttcccgataa
      541 catagataga agtaattgtt ttaaaattct tgaacagata gttgaagaaa attccatgat
      601 gtcaacctct agagtaaaaa gccatccatc ttttagagaa gtgttacctt ttattcaagc
      661 agactataaa caatggttag ctcactatcc tgaattaact tataccataa aagagggcga
      721 tcataaagga gttaccttac ttatatttga cgaatgtcca ttaagaaaac acacgaataa
      781 ttctaatggt tcttgtttgt ctgttacggc tgataataag attcgatttc attgtctgca
      841 tgcttcggat aaaaataaag atattcaaga ctttgtaaaa aaatatccgt tgccaaaacc
      901 tgtaaatcct tctgtagaat cattagaatc caatcaagaa tattattttg accagtttaa
      961 actgaatcaa gaaggagtat ttttggtgaa agaaggtcaa gaagaaaaag ttgcttgtcc
     1021 gatgtttatt aagcaaatac gcaaaaatat cgaacaaaaa gtcattacac ttgttcttgc
     1081 ctattatgtc gatcattgtt gggatgaaat agaaatagaa ggtggatgtt tacaaactaa
     1141 taattttaaa caattagcaa aacatggctt aatcattaaa gctagaagag aagctgaagt
     1201 cgttgacttt ttacagattc aacgggagaa tgctccaatc ttttttgaac atgaatctct
     1261 tggctggaaa aaagaaaatg aagcaatgat ttttcagtta caagattctt ttggacgtga
     1321 aattccttct gtattaacta aaaactcatg ctaccaatta acaactaatg gtacagaagc
     1381 ggaatttgac gcattggttc ataagtatgt tttaggaacg aacttagaac ttgcttggtc
     1441 gattggtatt gcttctattg tgttaggcta tttaaacgtt acgaaggcta atactttttt
     1501 gagtttacta attaacctac agggcaagag cacaacgggt aaaaccacta cgttacactt
     1561 tattgcctct ttatttggaa atccaactac ggttcttaga aatatgaatg ctaccaataa
     1621 agccattata aagctagcat caaacaatca gggaggtgtt ccactaatac tagatgagtt
     1681 aggagcagca actcaaaata actttagctc gttgatctat caattagctt caggagaaga
     1741 aaggctaaga ttagattcta attgtcagct tatgccacaa gagacttttt ctgttttgac
     1801 ccttacctct tctgaagagc gtttggaaaa ttatttcaat gagaaattaa ctggtttgaa
     1861 agttcgatat atcgaatttt ctgatatcaa gtttacaaag gatgcgaatc acgcagagaa
     1921 agttaaaagt ctttcttcta atacttttgg ttggggtata aagttatttc ttttgaaact
     1981 ctttgaacaa gggattacaa aaattgaagc aacttttgtc gaagctcaaa aaaaactatg
     2041 ggctgtttta cctgaacatt ctttgaagca acgcattggt aataatctag ctttgattta
     2101 tagtggagcc ctcctaattc aagatatact gggctatcct ttacgattag agcatataca
     2161 gacggtgctt attaattcgt acgaacagtt aattgtagat actagtttag gaagtgagca
     2221 aacggtattt gaccgagtga aaacattgtt ggtgatgaat agccataaat ttttaacaaa
     2281 cagtgacaac aaacatacgc ccagtacgat ttggggaaaa gtatcaattc aaaaaaatgg
     2341 ggatattaag gtaaatattt ttcctatccc gtttgaggac ttgttaaggc gagaatttca
     2401 agtaaaggat ctaacctatc tttttaggca attgcttgat tcgggtaata tgtgtgcaga
     2461 aaaagggaga agaacgaaac gtattcacat gaatcgtcaa gttcttccaa catacgagat
     2521 tctattacct tcttcactca aatcgtattt tcgattgtaa tttagaataa accaaagtat
     2581 gaaagtgagg caccatgtta tggaaatatt tcttattgat ttagcaaaac aactgattac
     2641 ttatctgtat gatagcgagc aaacccaaac ggaagtaaat gaagcttgat ctatgcttca
     2701 tttacttcat tcaatcaaac atgaaagaaa ggaaaattct aatgactaaa aataaaatga
     2761 cactaaaagc agaggttctt ctgtatattc aagaacattt ttcaaatcaa gcctttttta
     2821 caaaaccaat ttacttggat ttcgaaataa gaggggtatc tgctggatct atcgggggta
     2881 ctcttcaagc gttgaagaac gaaggatatt tagaaaatca ctttgtgcaa cgttcgttca
     2941 atagaagagt cgtgaaaaaa tggtatttag ttcattcata gagagggtaa tcaagcatgc
     3001 gtgtggcttg ttatgatcga acgtctgttt atgaagaagc gaaaattagt aatgacgctc
     3061 aatatgatgc agtgaaagag tatgttttag agaaatatga acaaattccc caacgatttt
     3121 cagacaaagg agaatcaggg gaaaaagcgg agcgtccagc ttttcgacag cttagagctt
     3181 gggtttgtgc gtcagaagaa gagggggtga tctatgtttg gcgttatgat cgattggcac
     3241 gtcaagccaa aatagcctta gaatttattg agttgtgcca acaacatcag gttcaagtgt
     3301 attcgatttc tgaacctctt cctgcttctt tctcacacgc gtcaacgaat aaactatttg
     3361 tgcaattatt gttcactttt gcagaacaac aacgaaacgt cattattgag aatgttcgtg
     3421 ccggactttc ctataaaaag caacaacggc aatatttgtc ttctgcggta ccatatgggt
     3481 atcgtttagt gaatgggaaa atccaacaag aagtacacga agcaaggatt gttcgctata
     3541 tttttcaact ttatttgacc aaaaaatatg gctacaaaaa gctttgtcaa cgattgacgc
     3601 aacaaaagct tttcttccgc gaacgtcctt ttcagcccta tcatatttat agtatcctaa
     3661 aaaatccatt gtactatggc gaaatcaaag gaggatcttt ggggaagtat ctaggaacct
     3721 ttgaaccgat tctctcgaaa gcaacctttt tacaagccca agagattcgc caatcaaggt
     3781 gtacagctaa aaaagatacg tatctgtact tattacgtca aaaaattcgg tgtcccttct
     3841 gtggtcgtca tctgagttcg aagtatcaat ggaatacaaa gaagacgaag acgcttcatt
     3901 attatcattg taccgaccga agctgccaag gaatttttct tcgggcagaa agggttgagt
     3961 cagaagtatt ggaaaagata caggcttttt ttcaatacga aggcgtctat caagccttga
     4021 ttgcccaatt aacgcaacaa ttagccgaaa agcgaaaaga tgaaaaacgc aaagtaatcc
     4081 aacaaaagaa acaagaagca caatggatta aggcatttga agagggcaat tgttcactgg
     4141 aagaactcaa gcaacaactg caagctcttc aaaagccgat gtctacttct tcgtttcttt
     4201 cactccaaca gtgtgaagca aaagtgaaag aatggcttca actaaaagac ctctctagtc
     4261 gacaattatt atgtgactat gtttgtcgga ttgaggtaac aaaggataaa acagtttgtg
     4321 ggatttactt ccgacaactg acacaaaata ttttagaaag gaacgaatga tctatggaaa
     4381 tgatacaaac caaacgagtc gcattgtata ttcgcgtgag tacacaagaa caagctttag
     4441 aaggacatag tattcaagca caagaacaaa atggacgaca gtttgccgaa cggatgggct
     4501 atgaagtggt agaagtttat gccgatgaag gaatcagtgg gaaatccaca aaaaatcggt
     4561 tcgcctatca acgaatgatg aacgatgctc ggaaccatcg ttttgatcta ctagtgattt
     4621 ggaaattgac ccgtctcgga cgcaatatgt tagatgtctt acaaacagtc gaggaattat
     4681 tgcgatatga cgtaggatta cattcgatct cggaacaatt tgacgtgacc acttcgacag
     4741 gtaaattgat gttacaactc cttgggagtt tcggtgaatt tgaacgcaac caaattgcgg
     4801 agaatgttca aatgtcaatg caaagcttag tgaaagataa aaagcgttat gcaggaggcc
     4861 gacgtttagg ctatgtcagt ggtattgaca cgaatggtca aaaacaattg gtcattgaac
     4921 cagaagaggc gaaaattgtt cgtctgatct atgcaaaata tttaggcgga gcagggtatc
     4981 gagcgattgc caattttttg aatcgtcaag gatatcaaac agtcaaaaag aatgcttttt
     5041 ctaccacggc agtcaaagat atcttacgca ataaaattta tgcagggtat ttagaatatg
     5101 cgcgctacat caattgggaa accaaacgac ggaaggggaa gaatccgaga ccgattttag
     5161 tgaaaggaac ccatgaaccg attatttcgg aagaagatta tcaaaaagta caagaacgcc
     5221 ttgctttaga aggaaaacaa cccaaatgga accatacagg tgaaaatgta ttgacaggct
     5281 tgttacgttg tccagaatgt ggcgcaccga tggcagcgag taatgtcaca aataccctaa
     5341 aggatggcac aaagaaacgg attcgatatt attcttgttc agtctttcgg aataaaggag
     5401 caagtgtgtg tcatgccaat tcgattcgtg ccgaacaagc ggaaacattt gtcgccgacc
     5461 gattaaagga gatcgttcag ctaccagaag ttcttccccg attggtggca gcgttaaatg
     5521 aagaaatagt gcgacaaagc caaccattgg aacaagaact cgtggtgtta ctggaacgaa
     5581 aggaagaatt gaagactaaa attgaaaaat gggaagctgc tttggaagat agtcctgaat
     5641 tgtttccgat gttaaaagac cgcttagacg aattgactga aaaacgacga cagttgcaca
     5701 ttcgagaaaa tgaaatctta gggatctttc aacaaggaga gccgattcaa gtcaaagatg
     5761 tccaacggat attgactagt ttggatcgtt ttcttgccca gagtgagaag aagcaaatca
     5821 aagccttgta tcgtaccttt atagagaaaa taacctttga tcctaaccat aaggagatgt
     5881 tggaaattac gatgaaattc acgcatgccg tggtacaaca attgaatgag caatatcaaa
     5941 tagccgtatc caaaacaaag gatacggcta tttttgtgtt aaaaacaccc tttacgttaa
     6001 cgatctagtc taggttatat caaacaacgt tagaggggcg ctaacgcttc tctagcgatt
     6061 tgaaatcgtt agagaagtag ttagggaggt ccttatcata aatagggata tttattaatg
     6121 aatatttgaa atataattgt atataagaat taaaaagagt ggggatagat atgaaaaaga
     6181 tgggtaagtg tattttatgt gaaaaataca ctgaacttac taaagaacat gtaccaccta
     6241 aaaaatcagg gaatactgga ggtaaaaaga ccagaactgg taatttagat gatttcctta
     6301 agagcgattt tacaaaaggt gattttccga aggggattaa aaggaaacct caaggaaatg
     6361 tttattata
//
LOCUS       DAZKCY010000129         6074 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000129 DAZKCY010000000
VERSION     DAZKCY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.130"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..541)
                     /locus_tag="ACG8XC_002395"
     CDS             complement(254..541)
                     /locus_tag="ACG8XC_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9192066.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="ACG8XC_002396"
     CDS             complement(561..785)
                     /locus_tag="ACG8XC_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFP9192067.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="ACG8XC_002397"
     CDS             complement(1240..1410)
                     /locus_tag="ACG8XC_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192068.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="ACG8XC_002398"
     CDS             complement(1425..1958)
                     /locus_tag="ACG8XC_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9192069.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="ACG8XC_002399"
     CDS             complement(1979..2923)
                     /locus_tag="ACG8XC_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9192070.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="ACG8XC_002400"
     CDS             3560..3646
                     /locus_tag="ACG8XC_002400"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFP9192071.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="ACG8XC_002401"
     CDS             complement(3767..4456)
                     /locus_tag="ACG8XC_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192072.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4701..5615)
                     /locus_tag="ACG8XC_002402"
     CDS             complement(4701..5615)
                     /locus_tag="ACG8XC_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP9192073.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="ACG8XC_002403"
     CDS             complement(5597..5977)
                     /locus_tag="ACG8XC_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFP9192074.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1610 a         1122 c         1021 g         2321 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa caaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagctcac taaa
//
LOCUS       DAZKCY010000130         6000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000130 DAZKCY010000000
VERSION     DAZKCY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.131"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(641..1144)
                     /locus_tag="ACG8XC_002404"
     CDS             complement(641..1144)
                     /locus_tag="ACG8XC_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9192075.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1503..2528
                     /locus_tag="ACG8XC_002405"
     CDS             1503..2528
                     /locus_tag="ACG8XC_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9192076.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2875..3753
                     /locus_tag="ACG8XC_002406"
     CDS             2875..3753
                     /locus_tag="ACG8XC_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFP9192077.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4229..4345
                     /locus_tag="ACG8XC_002407"
     CDS             4229..4345
                     /locus_tag="ACG8XC_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFP9192078.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5273..5611
                     /locus_tag="ACG8XC_002408"
     CDS             5273..5611
                     /locus_tag="ACG8XC_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192079.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5705..5935
                     /locus_tag="ACG8XC_002409"
     CDS             5705..5935
                     /locus_tag="ACG8XC_002409"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFP9192080.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         2048 a          894 c         1099 g         1959 t
ORIGIN      
        1 aaaattagtc aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga
       61 ataaatatta acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata
      121 acatatctca ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac
      181 cgtgtctcag atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt
      241 caaaaagtag gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag
      301 gagaaaaacc atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat
      361 aatatacctg agtagggtat aaattaagac ggaattcctt tctagattga gatattgact
      421 aggaagaatg ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag
      481 gggaatatag cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt
      541 agagatgaaa atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac
      601 attggaatgt gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc
      661 gggtcaaaaa taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg
      721 ctagacggtc taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac
      781 ctatcagtcc tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga
      841 ataggaattg aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag
      901 aaaaagaaag tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca
      961 agttataagt ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga
     1021 cgagattgac attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga
     1081 ataaaatcaa ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc
     1141 gcatagacat ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa
     1201 aagtatgaag tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt
     1261 gggaatccgg tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt
     1321 ttttctgtca aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac
     1381 tttttgatac taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca
     1441 aacgggattg gcatttcatc ttggttcagc tttttttcca attactagga aggagggata
     1501 gattgttcaa gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga
     1561 atctgatgct acttatcttt attgtattat ggttaggtta cataaatcat gattttttga
     1621 tttcaagttt ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga
     1681 aatatttgct gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg
     1741 ggatcttatc gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt
     1801 ccagcagatt cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga
     1861 tgctttctgc catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac
     1921 tttatttttt tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg
     1981 attcacggcc tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc
     2041 ctttagtgat cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca
     2101 gtcacggctt gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc
     2161 tagcagaaaa cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg
     2221 gtgggttatg tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt
     2281 ttctgatcac ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg
     2341 ctaatatttt tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact
     2401 tgatttcttt gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag
     2461 gtgttgcgtg ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat
     2521 tagaatagaa ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc
     2581 tctaattcaa ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg
     2641 tggaaaggaa ctttcaaaaa aattttatat ttattctatc aaaaaatttt atatttattc
     2701 tatcaaaaaa ttttgtttat gatggtaact gaataaactt tcctgagatg gtcaatgctt
     2761 tccttgaaag tcttacagat attctttctt catagaaagt ttaagtttta tagttagtgt
     2821 acaataaaag tatgtttata ccatatggtg tccggataat cagtcattta aacaatgagg
     2881 ttcaaagata tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat ccaaattctg
     2941 gcagaaactt taactcttcc caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac
     3001 atccttgatg aggtgtttga gcaatatagt catgaactaa tggatcctaa taaagcaaag
     3061 gcgactgcaa agaaaataga caaagtctat gaagaggaac cttttgcagc attagctatt
     3121 tatctatatg cagcttctca aagttggaaa aaaatttatc aaccattgaa gattccaaga
     3181 actatctatt tggcaacaat gaatgctttt actagattta ttcatgaaca tttccaagta
     3241 acaggaagct atcgctttga ccgtggtttt tggatttggc attacactag tggcttgatc
     3301 tttcggattg gtgaattgga gtacgaaatg acatattttc cgaaagggta cacggatcta
     3361 agattagaag gaaagcagtg cctttctctt catattcctt ccgatgcaga tttatctcag
     3421 aataagataa gagatagtta cgaaagtgcc attcgttttt ttgaacttta ttttcctgat
     3481 taccattacc aagtgatgta cacagatact tggttactat cacccaatct tactaaatgg
     3541 ttgaaaaaag agtcaaaaat ctgtcttttt gcagccgatt atcgattatt atcagttgat
     3601 gaacaggacg atagtggagt cccttggatc tttggacgag tagatgccca aatacatgat
     3661 tatccagaaa gtacgagttt acagcgacaa gcaaaagagc aattgcttgc aggcgagcac
     3721 attggctctg gcttaggtat tcttacttca taagtttagt acttcgaaat agtttaaaag
     3781 agcgtgagac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     3841 aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     3901 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttagtgt
     3961 tctatcgaac acacagtacg ttcaataggt aaactagcta taacaataga tattaaagtt
     4021 aacggacaag agggcctttt ttaggtagct aaaacgtaat aatgaaaatt tatttccaga
     4081 gataggagtc aatcagctag tctgatcgac tgttttaaca agttgacttt cattttaact
     4141 tttgatacca tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta
     4201 caatcaaaga agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg
     4261 ccgcttgaaa agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt
     4321 gaaacggaat agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa
     4381 acgtttttcg gctattttca ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt
     4441 tgattttcca tttttaacat atgataaatt ggtagaatct ttcttttcaa attgatacca
     4501 ttccctaatt ccagttatat agttaactac agaaccacaa agcacgtacg tttataaaag
     4561 tattatctgt agttattttt caattccctt tcaacctctt attttgacca gtagtattga
     4621 tcactttacc ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac
     4681 gtaactacat aatcaaaggt tttgcgaaat atttcatgaa atatttcatg tagaagcgag
     4741 caatacggtt gcaaataacc attatcgttt aataagcagt tacgaaagct cccaatatct
     4801 ctaacgttca gctcactcca tttcttttct tcaaaaaaat cagtgattcg tatctctttc
     4861 tatttgtcga aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa
     4921 aagcagttac tattgctaga ataacatcaa ggaataataa gttttggttt tcagataata
     4981 gtcgataagt tgtgtattgg aattttagat acagtaataa agtatttttc atgaaatttt
     5041 gtgagaatca ctattacaag tatctttaat aaaattaatg gttaaggagg aacttaaagt
     5101 agaatgtaaa tgaatagttg tagtcagtcg attgtgatac tagactcaaa gttgcaggaa
     5161 taagtttttc aatttgtgat tggaagataa aaaatgaaaa agaagggctt ttttttatac
     5221 tttgttgatt gtagtgttta ttttccctct gtttagccaa gtaacatttt ctatgactca
     5281 accagtttca agtgaggaac atgataacct ttttcgtgct acagaagagt ttaagaaata
     5341 tcaggaaaat attgttccgg agactccatt agctagtgag ccaattactg atattgatgg
     5401 aaaccaaata ggatggtata tacagtacga agttatggtg gtaaataatg actcaatagt
     5461 aaaaaataca gtaggcttag agctaacacc gattgaaaag gtgagttcat tgttaacgta
     5521 tatttatact gatgaaagtg acgagactga gatcatttta acggattact caaaaattgc
     5581 tactgataaa aagtatatat tgtggatgta gacactagtg aaatacttta tgatatagaa
     5641 attgatgaaa atttactaga tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt
     5701 cgatatgctg gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag caaaagttag
     5761 agaagatgaa aatgagtgga aagaattcaa gagtcgctac tcaattaact cgactccgac
     5821 atttacagtt tacagagaag gatcgataga aaaaactgtt ttttggacta aagaaagtgg
     5881 aatgtcttta gctgaagtag aagagttttt ggattatgtt agtatgcaac agtaagggag
     5941 ttcttattat gaaaaatttt gacacatatt atgttattgt tttttcgata tctttgctgt
//
LOCUS       DAZKCY010000131         5987 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000131 DAZKCY010000000
VERSION     DAZKCY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.132"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..452)
                     /locus_tag="ACG8XC_002410"
     CDS             complement(<1..452)
                     /locus_tag="ACG8XC_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9192081.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            676..1468
                     /locus_tag="ACG8XC_002411"
                     /pseudo
     CDS             676..1468
                     /locus_tag="ACG8XC_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
     gene            1434..2387
                     /locus_tag="ACG8XC_002412"
     CDS             1434..2387
                     /locus_tag="ACG8XC_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFP9192082.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2785..3759)
                     /locus_tag="ACG8XC_002413"
     CDS             complement(2785..3759)
                     /locus_tag="ACG8XC_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9192083.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3874..4308
                     /locus_tag="ACG8XC_002414"
     CDS             3874..4308
                     /locus_tag="ACG8XC_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9192084.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4433..5455)
                     /locus_tag="ACG8XC_002415"
     CDS             complement(4433..5455)
                     /locus_tag="ACG8XC_002415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFP9192085.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5847..>5987
                     /locus_tag="ACG8XC_002416"
     CDS             5847..>5987
                     /locus_tag="ACG8XC_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFP9192086.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRA"
BASE COUNT         1882 a         1129 c         1032 g         1944 t
ORIGIN      
        1 taatttgtaa taagttcgta tatgttttgc ggtgtttctt gtttcttgta attttccacg
       61 aatgtttcat ccatgaataa attcataaac gcttgaagta atcctacttg ttcttttggt
      121 tctttgattc ctaaaaaaaa tagattttct acagtcattt cctgattatc ccccatttga
      181 taaaaataaa ttgggttctt cagctgcata acaaaaacaa atggcttttc gacatattca
      241 cattccgaat gtggcagcgc gatatttaaa tacttagtaa tcaatccagt aggaaattct
      301 ctttctcttg caatcagtcc attcaaataa ttctcattga cgtaccccat tttttctaat
      361 tgttgtgcag cgattaaaaa agcttcttct ttatttttta cttctaccgc taattgaatc
      421 aaactaggaa cgaacatctc tttatatttc attccaacct cctccttttt gattaagttt
      481 gttaatttat tgttctacta agttcatctt tgttttacaa gaacattata tttctgttag
      541 tttgttttgt caacgctttc aaaagaaatt tattttgtta aatttgatct acttgaaaat
      601 aaaatcgttt atctatataa ttaagaagaa caaagtcgaa caaaaattga cttttatgtt
      661 aaaggagtaa ttcatatgct gaaaaaagaa agacaagaac aaatcattac attattagag
      721 aaagattttt atttaactaa ttcagaaatc gctaacaaat taggcgtatc agaaatgacc
      781 atacgccgag atgtcactga attagcaaaa aaaaaataaa gtaaaaaagc tatatggtgg
      841 cgttgaacga aaggaacctt ataatcgaga actatcgact caagaaaaga ttgatacgaa
      901 tgtagccgaa aaaaaatata ttggtcaagt catgaacagt atgattcccg ataattctac
      961 tatttatgtc ggtgctggaa caacgatcct atatgcactt cctttcattc aaaagaaaaa
     1021 tctatttgta attacaaaca gtttgattgc tttcaactat ttaaagaatc atacaagtta
     1081 tcggatttta cttactggtg gcgaattttc tcctattaca gaagagttca ttggggaagt
     1141 tgcagaaaga tcttttgaaa gtttgaatgt cgatatctct ttcgctgcaa caaacggcat
     1201 atttgataac aatgtgacaa ccgctcaatt tattgaggga aatattcaga acgcagcgct
     1261 caatgcagca aaaatcaaat gcattgttgc agattattca aaattaaata aatcagacgt
     1321 ctatactttt agaaaattaa ccgattttga ttatctaata accgataatc atttatcaga
     1381 acaggatttc aatcattatt caaaatactt aacaatatta aaggagccca aaaatgatac
     1441 tgacaatcac gatgaaccca tccattgatt ccatttattt tacaaaacac tttgaattag
     1501 gtacgatgac acgctttgat tctccggtac gctatgtagg cggcaaggga atcaattgcg
     1561 gacgagcttc tgctctacta ggttcatcag ttattttaac agggtttttg ggcggttatt
     1621 ttggcagtat gattcaagat atgctgaaaa atgaaaattt gtttgatttg cattttttac
     1681 ctattgcaga agaatcaaga ggtgccgtca cgatcatgca tgatgatgaa acgcagacag
     1741 agctagtgga agctggtcct gaaatagcag atactactat caaaaaatta atgaatgata
     1801 tcaaactaat cagtaaaaat aattaccttt cagtcattac aataaatggt tctgttaaca
     1861 atccagatcc ctttttgtat gctaaattat tacaatatat caaagaacaa atagatcctt
     1921 ccacctatat attaatggat atatctggac aacatctccg tgaagtctta gcaagtaagt
     1981 ataaaccgat gttcatcaaa cctaatatcc atgagttttc agatatcatc catcaaaaaa
     2041 ttactcaaaa aagcgacata aaacaccatc taaaaacaaa tccactattt aaagatatcg
     2101 atgttatcat gatttcttgc ggctcgtatg gtgcagtagt caaagtaaaa gaagaatttt
     2161 ggaatgttga gatccctaag attcctatca ttaatacaac aggctctggg gatgccactg
     2221 ttggaggttt tgctcattca ttagaacaga attatccttt aaaagaatgt gtaaggtacg
     2281 caatggcttg cggcatgtcg aatgcacagc aatcagaagt cgggaaagtt attccagctg
     2341 atgtaaaaaa tttagcaaaa agcattaaaa tcacagaaat gaaataaata gtacaaagta
     2401 aataattgtt tatatgaaat gagacacaaa gagactgtga caaaaaacgt gtgtaccgac
     2461 ccccaaaagt tagatttttt ggtctaactt ttgggagtcg atacattgtc acagtccttt
     2521 tttcgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat attcaacaaa
     2581 catgagaagc tattttttga aattgctcct tgtgactcag agttaaacgg caggctcacg
     2641 acctcttggt ataaacggtg ttcaaaagct tgcatctacg ctaatgagtc caaccaagtc
     2701 aaaaatgagg agcttttttg gctgtcctta ttacttgatg cagggcagtt ttttcataat
     2761 ctcttttaga ttaaatcctt ttggttacac aaggttctct tcaaagaatg ttgccatttt
     2821 ttcaaatgga atgatgtctg tctggtcgta tagatctgta tgggatgctc ctggaatgat
     2881 catgagttcc ttgttgtcac cttttagttc tttgaaagcg tcctccccaa agtaacgtga
     2941 atgagctttt tccccgtgaa tgactaagac tgcatgacgg atttctggaa tataatgcaa
     3001 tagttttgca ttcataagtg acaaccctga tgtcctattc caaccatcat tggagtttaa
     3061 agaacggaca tgatatccgc ggtctgtttt ataatacgca tgatagtctt tgacaaattg
     3121 aggagcatct tcaggcaacg gatcaaccac acctccagca cgtgcgtagt ccccttgttt
     3181 gaaatcaatg atgcgttgtg catttaattc ttcacgcatt tcatatcggg cattttcatc
     3241 cataacatcg aagtagccat ttgctgttac tcgagtcatg tcatacattg tggaaacaac
     3301 tgttgctttg acccgcgtat cgatagctgc tgcattcaat gccatacctc cccatccaca
     3361 aataccaatg attccgattc tctcagggtt tacatttttt tgtacagata aaaagtctac
     3421 tgccgcttgg aaatcttccg tattgatatc aggcgaagca acatatcgag gtactcctcc
     3481 gctttctcct gtaaacgatg gatcaaacgc aatcgttaaa aagcctcttt cagccatcgt
     3541 ctgcgcatat agaccagacg attgttcctt cactgcacca aatggcccgc tcacagcgat
     3601 tgccggcaat ttcttttcac ttacttcttt tggttcatat aaatcagcga ctaatgtgat
     3661 accaaaacga ttcgggaagc tcacctttcg atgattgact tgattgcttt ttggaaatac
     3721 tttgtcccat tcttgtgtga tgtttaattc tttattcata ttctagactc tcctttgatc
     3781 aaaattgaca tttgtttttg aatgtattat tatgataaaa cttaaacttg actttaggtc
     3841 aaggtaaaaa ttttatttta ggaggatttt tttatgtatt ccattggaca agtgtctgaa
     3901 atgtttggat tgcctgtttc tactttacgt tattacgaca aagagggatt atttcctgat
     3961 atccagcgtg tttctggtat tagaaaattt tcagagaatg agttagaggc tttaagagtc
     4021 attgactgtt tgaaaaaatc tggtttagaa ataaaagaca ttaagcaatt catggaatgg
     4081 agcaaagaag gcagtaagac atttgaggtt cgtaaagaac tatttgaaaa acaaaaagaa
     4141 gtagtagaaa aagaaatagc aaaattagaa agagtactcg ctatgctaaa ctacaaaagc
     4201 tggtactatg aagaagcaat aaaagccggc aatgaagaag ccgtattgac aatgattcct
     4261 gatgatttac cccaacatgt aaaagaagct tatgtacatt ctcattgaac aaacggataa
     4321 ctcactctcc ttctctatga acttcatgaa aacatgaata aacacctcta actgattgtt
     4381 agaagtgttt attttttcac tcaagtatcc tgcacttaca aaaatatcct ttttaccgcc
     4441 atcctaattc tggagcgata tcttttacga ttgtttcaag caagtgcatg ttatattcta
     4501 cccccaaagt attaggaatc gtgacaagta ctgtgtctgc ttctttgatt gcttcatctt
     4561 cagctagctc cttgattaat tgatcaggtt caccagcaaa agtttttcca aaaatcgttg
     4621 ggtttcggtg ataagcaaga tagccgacag catcttgttg cggactaccg tcttgtccaa
     4681 ataagcgtcg atccaaatca gtggtgattg gctgaatcga gcgagtaact aatgttcttg
     4741 gttcaaaatc atgacctgct tgcttccacg cttctttata cgcacgtaat tggtttgctt
     4801 gttggatatg gaatggctcg tgggattcgt aattgactaa tgtagatgat tgcagattca
     4861 ttccatgttg tgctgcccag attgccgttt gctgcgaacc agctccccac cagattcttt
     4921 gtcgtaatcc ttctgaataa ggttctatcc gtaattttcc tgatccttgt gggaacattg
     4981 gttgaggatt tggttcttca aatggttttc cttcaagtaa ttccaagaat tcaagggtgc
     5041 gttcccgtgt tacctctttg atttcttctt cactgtaatc ataaccaaag tatttccagc
     5101 catctagaac ttgttcaggt gaaccacgcc ccactcctag ttgcactctt ccttgcgtga
     5161 tgatatctgt caatccggca ttttctgcca tataataagg gttttcatat cgcatatcaa
     5221 taagtcctgt accgatttca atcttacttg tctttgcgcc gattgccgct agcaaaggaa
     5281 acggcgaccc aatctgaggg gcaaaatgat gtacgcggaa aaacgctccg tccactccga
     5341 ttttttctgc ttccaccgct aaatcaatcg attgcaaata agcatcttga gctgatttca
     5401 caagcgatcc ttgctccatc caatgtccaa agcttaaaaa gccgattttt ttcaaggtaa
     5461 tgacctcctt taaacctaca aaaatggaag gttttttatt tttaatatga actgattcta
     5521 ctactttttt tcttaaaaga cgaataattg ttctcgcctc acaatcaaga aatgtatcgg
     5581 cttactacac ccctactttc ttcatcatct gccgtgtctt aatctctttt aacagtctgt
     5641 tacatcgttg atttttgact caaaatgatt gtttctaaca tgtatttatg gtaaaaaaga
     5701 caagattagt tctttgtaca catcgattta tacgctgaac aaaaagaaat atataaacta
     5761 agaaacccat tgttttttcg ctccatcaca tagagtttac tttagataaa cagtgacatt
     5821 tactaatcca taacggagga aatcaaatga aaaaaatcta tttcttatgt acgggcaatt
     5881 catgccgcag tcaaatagca gaaggctacg cgcgtaaata cttgtctcct tcaatgtttg
     5941 agataagaag tgcggggatc gagacacatg gcttgaatcc aagagct
//
LOCUS       DAZKCY010000132         5922 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000132 DAZKCY010000000
VERSION     DAZKCY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.133"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..407
                     /locus_tag="ACG8XC_002417"
     CDS             <1..407
                     /locus_tag="ACG8XC_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192087.1"
                     /translation="QEKLERMNQLTTVMRTYVQTIKLVSLTTETDLTEQIVQKRQLLD
                     KNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDLQTRNR
                     QLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            570..2594
                     /locus_tag="ACG8XC_002418"
     CDS             570..2594
                     /locus_tag="ACG8XC_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFP9192088.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASRLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            2594..3799
                     /locus_tag="ACG8XC_002419"
     CDS             2594..3799
                     /locus_tag="ACG8XC_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9192089.1"
                     /translation="MENQDESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNDNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            3815..4459
                     /locus_tag="ACG8XC_002420"
     CDS             3815..4459
                     /locus_tag="ACG8XC_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192090.1"
                     /translation="MENKTLKIVLTAALSISLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNFSEAQAKIDKLNQTINEKNQEIDQLKQQDGIKAKEEVTDAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAETS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            4470..5276
                     /locus_tag="ACG8XC_002421"
     CDS             4470..5276
                     /locus_tag="ACG8XC_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFP9192091.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFTILGFLILASPIYTHKKVNI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQGLLKELANLDYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIVPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDDNYEQHAINYKVKGYKKEQKAAQKQIESLQATIDLNEELVNKLKDQLP
                     YQVADDQENTENKINDYQQEIETSKTQMKDQEEIIQKIQEKIDRMTKENI"
     gene            5299..5529
                     /locus_tag="ACG8XC_002422"
     CDS             5299..5529
                     /locus_tag="ACG8XC_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192092.1"
                     /translation="MKGANLVGKDIFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKIRSFEALYLAAKKYFTAERK"
     gene            5647..>5922
                     /locus_tag="ACG8XC_002423"
     CDS             5647..>5922
                     /locus_tag="ACG8XC_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9192093.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT         2163 a          945 c         1279 g         1535 t
ORIGIN      
        1 aacaagagaa actagaacga atgaatcagt tgaccaccgt catgcgtacg tatgtgcaaa
       61 caattaagtt agttagtttg accacagaga cggatctaac ggaacaaatc gtacaaaagc
      121 ggcaattatt agataagaat cgtatggaac aatccttagg aagaaatcta cagcgattaa
      181 gaaaatatga aaagaaatta gtagaggaaa tccaggaatt gaaacgagca gaacaagaac
      241 gtcctgactt aagcttcttt ttcgtcattg aaggaaagac aatcaaggat ttacaaacca
      301 gaaatcgtca gttaatgcga tctagtggca ttttagggtt aaaaccactc tacaaaaaag
      361 aattgatcaa gattctttat cgaatgaaca atatgaatga tgagtaggaa tggagaagag
      421 agaatgaaat atccgttgat atggttagaa aatgatgttc cgaaggaaga gatcactaga
      481 aaattttctc acactttaag cctgggtaag ttgggaaaag ggcaatcttt taaccaagta
      541 aagaatcaaa caagaagagg tgactgtaca tgttagaagc gattaaaaca gctttttcac
      601 cacaaaatga tcaaaaagta cttgatccat taaagcaact aggaaatcgg gaaatagaaa
      661 ttttaaaatc caaagggtat gatttagatt ttttaaaaca agtgatgcca caaggtggat
      721 taaatttcga tgaagagtat gtgacctatg gaaatggtta ttcacgatgt gtgacagtct
      781 atcactatac aaaaaatcct actctttttt ggttggccaa tctcatgaac aatcctcata
      841 cagtggccac actagatgtt agcacagatg aaaagcaaaa agtgattgat caaatcaacc
      901 gcgcattaga tgaattaaat gatcgatcag ataatgaacg aaaagcgact gaccgaaata
      961 aaagtgcgga agaatacctg gaactagcgg aatatgcccg aaaattgact tctaatgggg
     1021 aaatttctaa acaagtcaaa gtgagagtac atgtttacgg aaatacgata caagaagtgg
     1081 atgaacgggc gcaggaactg atcgaaaatc taaaagggat ggattatcgt gcggtgactt
     1141 atctgcataa aaccaaacga gattggcaat cgatgtttac catgttcgat gaacaagctg
     1201 aatggttcgg aaatatcaaa gggcaatcac taccatcgtt aaacattggc ggaggtttcc
     1261 cgttccacca ccaaacgcta aaagatccag gaggtacaca atatggggaa accatgacag
     1321 gcggcccatt tgtctggaat gcgacgtata aaaatagtgt acggttgtct actaatacct
     1381 tgcttctagg aatgatggga gctggtaaat cgaccgttct taaaatgatc gcggaagccc
     1441 atttagctgc aggagacttt atttggggct ttgaaaaggg aaaagacttt attcctttct
     1501 taaaggaata taacggtatt atggtgcgtt tagacggatc tgacgggatg atcaatcctt
     1561 tagagatatt tgccacacgg acgtatgatg aagcatccag gttatatgac gatggatcag
     1621 tcaaagattt gaagatcaat gaagcagcgt cctatcaaac gcacttagac aaagtagtct
     1681 atcaggtaca gttagtgagt cctcagctaa aaggaacgat gaaagcagaa tttaagacct
     1741 acttaaatcg tttttatgaa gagtatggaa cagtaccacg tggctttact tcctcaaatt
     1801 cgcgttcaaa tactgatacg caagtcacag gtaaagatcc agaagcttat ccgacgttca
     1861 aagaattttt agacttttta ggacaactgg aattaccagg tgcttcccaa gagaaaaaga
     1921 atcgaaaaga agaaatggaa tccatcgtag aatccttatg tgagacgtat ggcatgattt
     1981 ttgatggtca cagtacgatt cgacaccttg accaacaaca gttggtcttt tttgacattg
     2041 actctattac aggtttagat cgaggggtgc aacaatgtca aatgtacatg gctatgacgt
     2101 tgatttggaa tcaggcattg atccagggaa gaagacaacg acgtttaatt aaagaaaaac
     2161 aaatggaaga acaagacttc aaacgcttct tggcgatgat tgatgagtgc cataatgcga
     2221 ttaatccaga ttttattgaa acggtaaatt atgtcacaaa ctttcaacga gaaatgcgta
     2281 aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg ccagaaaata
     2341 tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct acgaagatct
     2401 ttatgcgtat ggatgaatct attttacgtc atatagaagg actagtgggt aaatcaatga
     2461 cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg atcaactttg
     2521 gatcaaaaga aaccattcgc gttcatttta gtccgaacaa acgtcaatta aaattgtttg
     2581 atggaggaaa ataatggaaa atcaagatga aagtttaatc aagcagtatg ttaagcgtcg
     2641 cgcaaagcgg cgcttatttt tgtggttgtt tggtacttct gcaggtttga ttactatttt
     2701 gatcactgtg tttgttaccc tatttctaat acttgcggcg ggttcgattg ataattcgga
     2761 ttcagattca agcagtggtg gcgaagcttt tacaggagaa tattcggaag gcttaccgat
     2821 ttataaagaa atcaaaggaa gaggcccttt ttctgatgag attgcgcaat atgccgtagg
     2881 tgcagcagtg aaatataaat tattaccaag tgtgattctt tcgcaatacg gttatgaatc
     2941 agcgtttggt acgtcggcgt ccgcaagaaa tgatctcaac tattttggga ttacttggtt
     3001 tgatggttgt ctgtttccta aaggaactgc acgaggaatt ggtggcattg aaggtggctg
     3061 gtacatgaag tttcccaact caaaagctgc attctcctat tacggcttta tggtagccac
     3121 acaatcaaat tttaatgcat gtgtaggaaa taaaagccct ggcgcaagct tattgatctt
     3181 aggacgaggt ggttatgccg ctgcaggaat tactgaagat agtccgtatt atacaggttg
     3241 tatgtcgatt attacctcaa ataaattaac agaatatgat gaatttgcga tcaaacattg
     3301 gggagaaggc ggaaatgaca acggtacgat tactggtgaa tggacgaatc ctttccctgg
     3361 cagcagttta gataaaagtt ccttcagcgg tggtcaatta tttggaacga atccaggcgg
     3421 tgaattccgt ccgaatggtt tccatgatgg attagacttt ggttcagttg atcatccagg
     3481 aagtgagatt catgcggttc atggaggaaa agtcgtgtac gttggaaatc ctggtatttc
     3541 aggtttgggc gcgtgtgtga tcgtgattaa ttacgacggc ttgaatatgg tatatcaaga
     3601 atttgcaaat agtacaggaa attcccgtgt gaaagtggga gatcaagtga aagttggtca
     3661 agtaatcggg atacgagata cagcacactt gcatttaggc ttcactcgaa tggattggcg
     3721 tcaagcacaa ggacacgcat tcacagatga tggtacatgg atcgatccat taccattttt
     3781 gaatagcagc aaaaaataga tcaggagaaa aaatatggaa aataaaacgt taaaaatcgt
     3841 cttaacagct gcactcagca ttagcttaat tggcaatatc gcgctctttt ggcagctgaa
     3901 agaggaacaa gcgacaaata cctataaaat tgaagaagtg gctaaaaacg cgtctgagaa
     3961 gctcaaaaaa aatttctctg aggcacaggc gaagatcgac aagttaaatc aaaccatcaa
     4021 tgagaaaaac caagaaatcg atcagctgaa gcaacaagac ggtataaaag caaaagaaga
     4081 agtcacagat gcccaaagaa agacggcaga agagttcggt caattggcca ttgataaatc
     4141 actaagcaga aatgaattaa cggaaaaact aaaagatgtc gcaacccaag aagtgatcga
     4201 caaattaagt cctgcagatg atgatcacca acatgatgat tatggaagtt actcctttac
     4261 attaggtgac gtgcaaactt acgctgaaac aagcagtgta aaagaggaac aaaattttgt
     4321 cgtatttgtt gattatacaa tcggcaatcc tcaattcaaa gatgtcaaac cgcaaaaaat
     4381 aaaaggtgga ttaactgtca ctgaaaaaca agtagatggt caatggaaag tcacagactt
     4441 tagttatttc actcgttagg aggaagaaaa tgagtaagaa aaaaggagca agacaacgtt
     4501 ttaaaaaacg gaaaagttat cttagatggc tgggaatctt tacgattctc ggctttttga
     4561 ttcttgctag tccgatttat acgcataaga aggtaaacat accagatgag gtaagcacga
     4621 acgctttttt aaacacgaaa gaactgacaa tggtatcaaa agaaaaagga gcgaatcagc
     4681 aattagtttt tgaatttgcg attgacgatg aggatcaagg cttattaaaa gaactggcta
     4741 atcttgatta tcgcgtagaa gtaaagacag ctaaaggtga ttatcaagca ataaaagcca
     4801 aagtgatcaa ggtgtctgat gattattttg tagtgaaatt agcacatgta ccagaagaat
     4861 atatcgccat gcgtttaacc attgttcctg aaaaaattga tccaaaagtg gatatgcaag
     4921 aaccacaaga tctgatttat tacgttcacg aagataaggt taaggatcaa gtgaaagatg
     4981 ataattatga acaacatgcg atcaattaca aagtgaaagg atacaagaaa gaacaaaaag
     5041 cagcacaaaa acagattgaa tccttgcaag caaccattga tttgaatgaa gaattagtga
     5101 ataaactaaa agaccaattg ccttatcaag tcgcagatga tcaggaaaat actgaaaata
     5161 agattaatga ttaccagcaa gaaattgaaa catcgaaaac gcaaatgaaa gatcaagaag
     5221 agatcattca aaaaattcaa gaaaaaattg atcgtatgac aaaagaaaat atttagtaga
     5281 acaggtggag tgaaagaaat gaagggagcg aacttggtcg gcaaagatat tttcgctgag
     5341 cagaaagtat tgattaaagc ctatctcaaa gttgtaaaag ctaaagaaat atgtcaagaa
     5401 gtgaatttat ctaacacaac aatatctgag ttgaagaatg gtagaaagag tttagatacc
     5461 ttaaaaatca gatcatttga agctttgtat ttagctgcta aaaaatattt tacggctgaa
     5521 agaaaataaa aacaggagga ttttatgtga cgcgaattgt aaaattaagt tagaaaaata
     5581 aaaaggcatt tatggtacac tcaaattgta tttccgacga aagaaaacaa aggagtgtaa
     5641 ccataaatga cctacaaaca tcttaccata gacgaactga caatgataga atcatattat
     5701 cttcaacata ataagccggt tgaaatcgct aaccgaatgg gacgtgctat acaaactatt
     5761 tataatgtag tcaataagtt caagcaaggc aagactgctc ttgattattg gcaccagtat
     5821 aaagaaaata agaaaaaatg tggtagaaaa gtcattcaat tacctgctca tgaagtagat
     5881 tacattaaag agaaagtcac tcttggttgg acgcctgacg tc
//
LOCUS       DAZKCY010000133         5916 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000133 DAZKCY010000000
VERSION     DAZKCY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.134"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..288
                     /locus_tag="ACG8XC_002424"
     CDS             <1..288
                     /locus_tag="ACG8XC_002424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192094.1"
                     /translation="TKVSSINTCSSYVQLRKSRYRGTFFFAAFFGLMRSTFAGRIRYK
                     TSFFTKDINRVKETSFCCQFFHFSKNENKKFWKWRRILLAILCLVEKRWTK"
     gene            362..1873
                     /gene="gadC"
                     /locus_tag="ACG8XC_002425"
     CDS             362..1873
                     /gene="gadC"
                     /locus_tag="ACG8XC_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFP9192095.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1886..3286
                     /locus_tag="ACG8XC_002426"
     CDS             1886..3286
                     /locus_tag="ACG8XC_002426"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP9192096.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3451..4886)
                     /locus_tag="ACG8XC_002427"
                     /pseudo
     CDS             complement(3451..4886)
                     /locus_tag="ACG8XC_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            5279..5872
                     /locus_tag="ACG8XC_002428"
     CDS             5279..5872
                     /locus_tag="ACG8XC_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP9192097.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVSGGMATVFLTNF
                     IRCRTSFRMLFRLSLTMSLLGCDDLRQELTVKVRLPR"
BASE COUNT         1880 a          961 c         1132 g         1943 t
ORIGIN      
        1 acaaaagtat cttcgattaa tacttgctca agttatgtgc agctacgaaa aagtaggtac
       61 cgaggtactt tctttttcgc agcttttttt ggacttatga gaagtacatt cgctggaaga
      121 atacgatata aaacgagctt tttcactaaa gatattaata gagtgaaaga aacaagcttt
      181 tgttgtcagt tttttcactt ttctaaaaat gaaaataaaa aattctggaa gtggagaaga
      241 attttgcttg ctatactttg tcttgtagaa aagaggtgga caaaataatg caagctcaaa
      301 taagcgattt tccataacct tggtaggaat gacaaattaa ataggaaagg gaagtaataa
      361 tatgaataaa aaaaagttat ctctattcgg tttttttgcc ctgacagctt ccatggtttt
      421 gactgtttat gagtatccga cctttgcaac atcacaatta catttggttt tctttcttct
      481 tttgggaggt attttatggt ttctaccagt tgctttatgt gctgcagaaa tggcaacagt
      541 tgaaggatgg aaagatggtg ggattttcag ttgggttagt caaactttag gagaacgttt
      601 tggttttgca gctatttttt tccaatggtt tcaaattaca gtaggtttta ttacaatgat
      661 ctacttcata ctaggagcgc tgtcgtatgt aattaatttt ccagagctta atacagatcc
      721 atttatcaag tttattggtg tattagtcat tttctgggta ctgacttttt cacagttggg
      781 cgggaccaat cgtaccgcta aaattgctaa agcaggattt gtcattggaa ttgttattcc
      841 ttcgatcatt ctctttggtt tagcagcagc ctactttatc ggcggtaatc cgattcaaat
      901 ggctattaat gcgaaagctt ttattcctga cttttctcag ctttcaactt tagtagtatt
      961 tgtatctttt atattggctt atatgggcgt agaagcttca gcatcacata ttaatgagct
     1021 ggaaaatccc caaagaaact acccattggc aatgttttta ctagtgattt tggccatcgc
     1081 cttagatgca atcggcggtt tctcagtagc agcggttatt ccacaacatg acttgtctct
     1141 cagtgctggt gtaatccaaa catttgaaac gcttatttta cattttaaca gtcatttaca
     1201 ttggttggta aaagtgattg ctctcatgat tgcctgtggc gttatgggtg aagttagttc
     1261 ttgggtagtt ggtccatcac gagggatgtt tgcagctgct cagcgaggtc tgttaccagt
     1321 tttcttccgg aaaactaata aacaagacgt tccagttccg ttgattatga tacaaggaat
     1381 tatcgttact atttgggcag cagtcttaac ttttggtgga ggaggcaaca atttatcatt
     1441 cttagtagca atctccttaa ctgttgtcat ttatctcgta ggatatttac tatttttcct
     1501 aggttacttt gtattagttt ttaagaaaaa agacttaaaa cgaacttaca atgtaccagg
     1561 aaaaactgtt ggcaaaacga ttatcggtgg aattggtttt attttatcag tgttcgcatt
     1621 gcttatctct tttgtaccac cagcttcaat tgcagctagt gagcaccgaa cataccaaat
     1681 catactactt attagctttg tagtgactgt tcttttacct tttatcattt atgaagtaca
     1741 tgataaaagt gctcacacaa ctattgagga acctactcac gtgaaagctg aagacgttaa
     1801 tcccgctgtt tatccagctg ctagaggaga gcaccacgtt acaaaaaatg atgaacatat
     1861 tttaaaacat taggaggaat tcattatgtt atacggaaaa gataatcaag aagaaaaaaa
     1921 ctatttggaa ccaatttttg gctctgcaag tgaggatgtt gacttgccta aatataagtt
     1981 aaacaaagaa tccattgaac cacgaattgc ttatcaatta gtacaagacg agatgttgga
     2041 tgaaggaaat gcgcgattaa acttagctac tttttgtcaa acgtatatgg aacctgaagc
     2101 agtgaaattg atgacccaaa cgttagaaaa aaatgcaatt gataaatcag aatacccacg
     2161 aacaacggaa attgaaaacc gctgtgtaaa tatgattgct gatttatggc atgctccaaa
     2221 taatgaaaaa ttcatgggaa cttcaacgat cggctcttca gaagcctgca tgctgggtgg
     2281 tatggccatg aaatttgctt ggcgtaaacg tgctgaaaaa ttaggtcttg atattcaagc
     2341 aaaaaaacct aacctggtga tctcttctgg ttaccaagtt tgttgggaaa aattctgtgt
     2401 atattgggat gtggaactga gagaagtccc aatggatgaa aaacatatgt caattaatct
     2461 agatactgtc atggattatg tggatgagta cacaattggt attgtaggta ttatgggtat
     2521 tacttacact ggtcgttatg atgatatcaa gggtctgaat gatttagttg aagctcacaa
     2581 taaacaaact gactataaag tatacattca tgttgacgct gcatcgggtg gcttttatgc
     2641 accatttact gaacctgatc tagtttggga ttttcaattg aaaaatgtta tctcaattaa
     2701 ttcttcaggt cacaaatatg gtttggtata tccaggtgtg ggttgggtct tatggcgtga
     2761 ccaacaatac ttaccagaag aattagtatt taaagttagt tacttaggtg gagaaatgcc
     2821 aactatggct atcaacttct ctcatagtgc agcacaacta attgggcaat actacaactt
     2881 tgtacgctat ggctttgatg gttatcgtga tattcaccaa agaactcatg atgttgctgt
     2941 ttatttagcc aaagagatcg aaaaaactgg tatttttgaa atcattaatg atggatcaga
     3001 attgccagtt gtgtgctata agctgaaaga agatcccaat cgcgaatgga cactatatga
     3061 tttatctgat cgtctgttaa tgaagggatg gcaagtccca gcctacccat tacctaaaga
     3121 cttggatcaa ttaattattc aacgcttagt tgttcgagca gactttggaa tgaacatggc
     3181 tggtgattat gtacaagata tgaaccaagc aattgaagag ttgaataaag ctcatattgt
     3241 ttatcataaa aaacaggatg tgaaaacgta cggctttact cactaaaatt cttcagagaa
     3301 atataaaaga agttaattaa ctaataaaag gagttgtgat taccacacag ctccttaaat
     3361 ctatttgaat aaatatatga tattcatttg ttacactctc aatcttagct gttgttctat
     3421 tcatcattac tcttattgta agtagttcag tcaaatttgc ttgttcaatt gatcgataac
     3481 agtatgaatt gtttgaaaag taatcggata tataattgtt ttctcactac ctaagccttc
     3541 ttggtataaa gaactatcgc aaagtataat ccgctgagta caaaagaaag tgtcgtctac
     3601 tatttctcta agttgtttac aaatgatatt actaatagaa aagtgttctc ccaaaatgtc
     3661 ttctaactgt tgtcgatagt ataaatacct aaagtctgac ggagctacta tgaacacttc
     3721 aattggagat ttccgtttat gctcatttaa aagacttaaa ctaatggtta gtttacgaat
     3781 agaattctca ttccaacgca gaaataaatt gttttgtttt cgccattgtt caagaatttc
     3841 atgaacttct aagtacaaag cgcgttgctc ttcatttaac aagtaagtct tatcaggttg
     3901 aaatatctga atatcagccc aaagtgagcg gataaacggg agaagtgctt tatcaaaaag
     3961 tatctgattt tcttcagata tattcagaga taagataagt ttttgactga gctcaatcac
     4021 caaagggtta tctgctacat ggttttgata aacaacgttc aagtcttttt gtaacaattc
     4081 aaagttatcg catagataat ttctagagtt gaatagaaag tagatataaa ggcattcatc
     4141 ttcgtcatga cataaagaga aagaaagttg ctctaatcct ttctgtatta gctgaaacaa
     4201 gggagtatgt cgggcaactc ttttttcttc ctgactaaaa tatattgggt tagtcttaga
     4261 tctctgcaaa ccgatagcga tacctaataa aataaattgt ttttcaactg tagggtaact
     4321 tcttgaaaaa aatgtttttc tacataatca atcaattgtt gaccgctttg aataattgat
     4381 ttatttagct ggatcaactc tgtccgattt gtctcataga ttatcgttag taacaaactt
     4441 cgataatcct tttcaggaat ttcaaaatgg ccatcagcat ttaagtactg catttgttta
     4501 aaaaaattaa tcaactcatt tcgtaagttg taaactttgc ttaacgaaat gaaggtagta
     4561 tcagctatat tactccaatt gtgttcccct gataaataat gtcctagtac attgaaaaag
     4621 tcagagcgct gatagatgag ttgagtgagt gccaacctcg aaaacgaaga gtcgaatgcg
     4681 actgtaaagc tacttctaga tttctccact gaaataattt gctgatggag tatgtcggta
     4741 atgcattcca agtcgttagt gacagtgagt gggcaaacgt tcaattgatc tgctatttcg
     4801 cgaatctgaa tggtcccttt ttcgtgtaat tgctcacaaa tgtaaacttg acgaaaaata
     4861 cttttctcca gataggtttc caacatagta ctcctcctag tcattttata cataactatt
     4921 ataactctac caatcggaat agtcctgtaa tatattgaat caaaaatata aattataaaa
     4981 taattagatg gggtatttgg tttaagtatc ccttatatag taataaattt ataggtgtga
     5041 ttctcgaatt aatacccgaa aatgtgagag aatttttatg gattttttag gatacaaatt
     5101 aattattttt gcttttatcc tgattaccag tccagtactg tcggtatccc attttgcagc
     5161 aaaatcatgt tgataaagtg acaagaagac tgatgagata tattatccaa attgagaaga
     5221 taattaatcg gattcttgag tataccctac tttttaagaa agtagggtat acaagcgtat
     5281 gaataaagac ataactttcc ctgtgatgga cgttcggctt gttccaatta ccaaaataca
     5341 ggcgaacgat tataatccca atcgagtagc gtctccggaa atgaacttat tggagctttc
     5401 tatcattgaa gatggcttta ctcaaccatt agtttgttat tacaaggaaa aagaagagaa
     5461 gtatatcttg gttgatgtgc ttcataggta tcgtgttgct aaggaaagac tcaacttaaa
     5521 agttgtaccg gtaacagtta ttgataaacc catagaagaa cgtatggctt caacaattcg
     5581 acacaatcgt gcaagaggga cccatgaaat aagaggaatg gaaattgtca tccaaagatt
     5641 agtaaaaaat ggatggtcag acaagagaat atccaaagaa ttaggcatgg acattgaaga
     5701 aatttttcgc tttaagcaag tgtctggtgg aatggcaaca gtttttttga caaattttat
     5761 aaggtgcaga acttctttcc gtatgctatt ccgattgtcc ttgacaatga gcctcctcgg
     5821 atgtgacgat cttcggcagg agctaacagt taaagttaga ctgcctcgct agcgttagca
     5881 catccgagct cattatcaag gattcgctgc gccaat
//
LOCUS       DAZKCY010000134         5874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000134 DAZKCY010000000
VERSION     DAZKCY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.135"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            641..1375
                     /locus_tag="ACG8XC_002429"
     CDS             641..1375
                     /locus_tag="ACG8XC_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9192098.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="ACG8XC_002430"
     CDS             1391..2560
                     /locus_tag="ACG8XC_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9192099.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="ACG8XC_002431"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="ACG8XC_002431"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFP9192100.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="ACG8XC_002432"
     CDS             3650..4576
                     /locus_tag="ACG8XC_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFP9192101.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="ACG8XC_002433"
     CDS             4595..5845
                     /locus_tag="ACG8XC_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9192102.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1966 a          955 c         1203 g         1750 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gtta
//
LOCUS       DAZKCY010000135         5716 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000135 DAZKCY010000000
VERSION     DAZKCY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.136"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..303
                     /locus_tag="ACG8XC_002434"
     CDS             <1..303
                     /locus_tag="ACG8XC_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9192103.1"
                     /translation="LGVKGQNLLADDSFWQIEAFYLAQCAYNTTLMLSPDRIIFGGGV
                     MKQEHMKKKVQDKFVELINGYVEIPPIASYIVTPELGDSAGIIGGLALARKAIRNK"
     gene            complement(1794..2459)
                     /locus_tag="ACG8XC_002435"
     CDS             complement(1794..2459)
                     /locus_tag="ACG8XC_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9192104.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2585..4621
                     /locus_tag="ACG8XC_002436"
     CDS             2585..4621
                     /locus_tag="ACG8XC_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFP9192105.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4625..5263
                     /locus_tag="ACG8XC_002437"
     CDS             4625..5263
                     /locus_tag="ACG8XC_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9192106.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5291..>5716
                     /locus_tag="ACG8XC_002438"
     CDS             5291..>5716
                     /locus_tag="ACG8XC_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192107.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1872 a          878 c         1061 g         1905 t
ORIGIN      
        1 ttgggtgtaa aaggccagaa tttattggct gacgattcgt tttggcagat tgaagccttt
       61 taccttgccc aatgtgcata caatacaaca ctgatgcttt cgccagatag aattattttt
      121 ggcggtggtg taatgaaaca agaacacatg aagaaaaaag ttcaggataa atttgttgag
      181 cttataaatg gatatgttga aataccacca attgctagct acattgttac gcccgaatta
      241 ggtgatagtg ctggaataat cggcggattg gcattggcta gaaaggcgat aagaaataag
      301 taattttaat gaataaatgt ttcattggta ctcatgtcaa aagtatttaa ctgagtaatc
      361 aaatgatgtt tgataaagcg aaaatgaatt caaataaaaa aggatcgagc aaactggaac
      421 atttgtccga tcctttttca cattctattt atttcgttta ttcaaccaat cggtagagac
      481 atccattgcg ctcttgtcgc gtggctttga tccatataat tgaccattac gaaatcatgg
      541 atcatcgctt tgaatgcgaa cttgtgtcac atctactttt ttgaccagat tttgaccagt
      601 ctattttaca ttctaatgta cttatatgag ttaatttttt gagtaaatgg taagtttttc
      661 cttgtttgtt ttatcttaaa ctagtgtgta atttgaaaat ctatatggaa gggtctgtgg
      721 ggtaatactt aaataaaggc taggaacctg ataacatcaa ggttcctagc ctttttataa
      781 taaaatgaag cgaaaataat ttcttttaaa gagccgagat tatcagctat ataagagtga
      841 atttgatgca aaaaacagag ccgaaacagt cttggtgttc gtagctctgc ttatatgttt
      901 cgaatttaag acaaagagtt ggcaaaagat gttgataagg aagataagat aactgtgtta
      961 tataaactag gtaactaact gtaacaagct aattttaaga gattcttatt agaagacgtt
     1021 taggaatata actaagaaaa tgattataca ggtaattgtc atttcaataa tgttttctaa
     1081 ttttcgcgtc tttgttttaa gaaaaaggcc agaatcagtt ggaaaaaagt tcgtacgata
     1141 gtgaagaggg gcaaaatttc ctactaatat tcccactaac aagagataaa acggccaggt
     1201 tggtgtgatt cctctataaa aaaatgccca tgctaccgaa atcccataga ttaacaactg
     1261 aacaattttt acaatgaaaa aattgttctg ccattactca aagtctcttc ctccttgtat
     1321 cgtttatttg tttcatttta tcattatgat aggcgcttga acaatccatt ttgtatttta
     1381 ttgagtactc ttggataaac taccagcgat tatcatattt atttatgtta ctgtaagcca
     1441 tcatataaaa gtaaatgagc agatcataga aatataaaaa agaactaaaa ttgatctaaa
     1501 ttatgtgcag cttgactacg taccaatcta tattacttac tagattggtc gttaatctat
     1561 tcggatcgaa tgagcaaaat atttttgtga atgtccatcc ttgattatga ttccaaaaag
     1621 aatcattgga aggctgtggc gtaaatatta tactataagc cagaaatacc tatcattctg
     1681 aactttttta tttaggaact ttcatctcaa ggctttttca attaatttgt acccctgttt
     1741 tttggataag atcatgtgca ttgatttttt ctatctctac gttcatctcc tacttaatgc
     1801 ctatctggct cctgagggct tctttttaag gcgtcgtcat agtcttggct gaggatttct
     1861 tggtaatctg agggaatggc ttcccaagtg agcaggcgaa cgttcatgac gtgttttcct
     1921 ttgtgtttga ttacagcatg gtttttctct ttttctacct ctccagcgtg aaaagacagc
     1981 catgtaccat ggccaaagat atcattacat acccaacgtt cgatggaatt gtccacgtat
     2041 cctacaggga gtgtggtata gccgatttgt ggtttcaaaa aaggattgaa tcgatcgatc
     2101 gtttcatttt tgtattgata attgataaga aaaagataga ccaaacaaaa gacgagcaga
     2161 aagaaagata gtgtcttttt gagtgagaag ggttgttttg atgaataaga tttcttgtta
     2221 acaagaggtt catctggcaa tgttggttct attgattcgg tagctgattc aatagaagga
     2281 aaatcaatcg gcagacctaa agtatgatgt aaaagtgaca gcatttcaaa gtctggcatt
     2341 gtacgagaat tttcccaact agaaatcgtt ttatcggaaa caccaatttt ttcgcctaat
     2401 tctttttgcg taagcttaca atttctgcgg taagttcgta ttttttctcc ggtgttcatc
     2461 ttcttctcct ttcgattcta cagtatatag atttattcta catgacctat acacttgata
     2521 gatacgtttt tttgatgaaa aacggtatga ttttatcaaa gaatcattta agtaagggag
     2581 agtggtgata atcaaaaaaa agttcacagt tttactcaca atcatagtga ccggtatcat
     2641 gagtggtttt gccttggtta gtataaataa acaggtactc tatcggacaa atgaattttt
     2701 ccatcaaaca gatacttata ctccgattcg attgccacaa cattttattg ggcaagaaga
     2761 atatgagcag ctaatcatgg tatttgaaaa tgtctcatca gaaactgagc tttcttatat
     2821 gaaaaaaaca gtaaatgaat ccaatgaagt agatacatct tggaacttca agatacagcc
     2881 aaagcttgaa atgacattgt attatcaagg ggatgtcaaa tcttctcacg agaaggtacc
     2941 ctattctaat acctcctttc gcgtaacgtc cattgaagaa tcgatcaaaa taaaagattt
     3001 tgaaggtgaa tttttcttga tgacgagaga aaaagaagcc tatgaacggg tcatttcact
     3061 attcactgat cgattcaatc aagcatttca cactgattat gccactaata tttttgaaga
     3121 ctttgaaaat agctattcgt attcggttat ttcaaataca gacgtatata attatcgagt
     3181 gtatttaact attggattga ttcttttatt ccttttgtta tcatcctttt tcttattgat
     3241 gaataaagaa atcagtttat tgttgaataa tgacttttca ttacatcaag tattgtggtg
     3301 tttattgggg aaaaagaaaa tcattattac attcataatt gcaggaatca ttggtggttt
     3361 tatttttatg tattataagg agcaatttct ttatttgttc cagatgtacg ttttcttgtt
     3421 cagcggaata tatggtatct ctttgatttc tcttttatta gttgaaaaaa tcgcttcgtt
     3481 aagagaagcg ttcaactata tccaatacat tctttttaca tttggattgt tgttttctct
     3541 tcttttgacc gttgccaatg tcgaattagc aactatcgtt ttttcttctt cacagttagc
     3601 tgtttcccat gaaccaccaa aagaaatcgc tggtgaggac tatcatgtat tttatccagt
     3661 aaccatcggg aaaaaccacg tggattttat ttattccaat cgttttgcat cagcagcaga
     3721 tcaggaatta tatgaaactt tgaataaaaa cggaagtata ttagtcaatg tatcagatta
     3781 cttgaatgaa gaaaatgaac agtttggcag agggataaaa ataaatctga attatcttaa
     3841 gaaatttcct ttgattgatg tctctggaag gttgatccaa ataaccgaaa aagaaacgca
     3901 ccctgttata ttgatccctg aacgatatag acatacagat ttaaaaaatg acctggcgca
     3961 aataacagaa tattatcaag aaatcagtga aaaagaaccg aaatttctat ttataaaaaa
     4021 caatcaaccc atttatacat ttattccttc tatcccctgg atcgaacatt atccggttgt
     4081 tgaaatactg acattgaaaa acagtatgta tttggaaaga aatattctca gcggagaaat
     4141 ctatccaccg ttaaagatca agataggctc tttgtcgaaa gaaaggttat ttgagctgat
     4201 tgaagaaagt cagttacgtg ataacttgca gttatcattt ccgtatactc agactgaaga
     4261 gatagctgtc aaagtgttaa gcagatcatt tcattacttg atacagatat ttttactcac
     4321 tttcttttct ttcttcttgt tgagctatgt catgttatgc atatattttg tctacaattg
     4381 tcggaaaatc gctatctttc gttcaagtgg ttattcgctc tttgaaacgt ataaagattt
     4441 cttcatgatg aacctcatca aatggggaac gacaagtgta atcttccttt ttcttataga
     4501 aagagagcct aaatatcttt ttaatatttt tttctttagc ttcattgatt ttattctttc
     4561 agttgttttt attttgtcta cagaaaagaa aagtcagtta ttgctgatga atggaggtta
     4621 acaattggag ccgttgatag tgataaaagg tgcgaaaaaa atcattcgaa acaaaacgat
     4681 tcttcatttg gatcgtttca ctgcggatgc tggggatctt gtgactgttg ttggagaaag
     4741 tggagcaggt aaaagtacgt tattgaatat attaggattg aatgatcagt tcagttcagg
     4801 acaattctat ttgttcgatc aagagacgaa acaattaact gggagagaga agcgcttgat
     4861 aaaaagaaat accatatctt ttctttttca agattttggg ttgattgaat atgaaacaat
     4921 cgattttaat ttagatattg gattgaagtt ttcgaattta aatgtttcag ataagcgaac
     4981 agcaaagtat gaggcattga aaaaagtaaa tataaacaag aagctaagta cgcctatcta
     5041 cacattatct ggtggtgaga agcaaagaat cgcctgtgcg agagtcttat tgaagaatag
     5101 caaaatcatt ttagccgatg aaccgactgg gtcattggat aaaagaaata aggataaagt
     5161 agttgaaatc ttatcgcaac aagcttccca aggtacgtta gttatcgtag tcacgcatga
     5221 tccatatctg accaaaatag gaaacaagaa gatcatgctt taaaaatcaa aaaggaaagg
     5281 atgtcgtttt atgaaaaaag cagttattat ttgtgcacta gggataggtt ttgttggagg
     5341 aaacgctatg tcatctgaag tggaagcaaa ttcagttcac ggtgaaatct attacaaatc
     5401 tttaaagcca ggagagattc attttgatgc taaaggtaat attattaagt caaatttacc
     5461 agtagaccgg gaaatttcta gaacagcaat taatcatagc tctggaagat gggtttatta
     5521 tagtgataca acttgtacga gaaaaacagg tcattctaac cactatagtc gtgtctataa
     5581 tcgtcacggt gcaaaagcga aagttggaaa ctctattcga tcagcaaatg caacaaaagg
     5641 agtatgggcc tatgcaagtg cgacaggaaa tagaactgac acattctcat gttggtacaa
     5701 tccaaccggt tggtat
//
LOCUS       DAZKCY010000136         5683 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000136 DAZKCY010000000
VERSION     DAZKCY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.137"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..613)
                     /locus_tag="ACG8XC_002439"
                     /pseudo
     CDS             complement(1..613)
                     /locus_tag="ACG8XC_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            761..1902
                     /locus_tag="ACG8XC_002440"
                     /pseudo
     CDS             761..1902
                     /locus_tag="ACG8XC_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2028..3107)
                     /locus_tag="ACG8XC_002441"
     CDS             complement(2028..3107)
                     /locus_tag="ACG8XC_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9192108.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3216..3656)
                     /locus_tag="ACG8XC_002442"
     CDS             complement(3216..3656)
                     /locus_tag="ACG8XC_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9192109.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3713..4123)
                     /locus_tag="ACG8XC_002443"
     CDS             complement(3713..4123)
                     /locus_tag="ACG8XC_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP9192110.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4304..4738)
                     /locus_tag="ACG8XC_002444"
     CDS             complement(4304..4738)
                     /locus_tag="ACG8XC_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9192111.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4838..>5683)
                     /locus_tag="ACG8XC_002445"
     CDS             complement(4838..>5683)
                     /locus_tag="ACG8XC_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9192112.1"
                     /translation="KETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGIL
                     LEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITSD
                     RLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVSL
                     NLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIGR
                     GLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGFT
                     YHPIE"
BASE COUNT         2071 a         1061 c          948 g         1603 t
ORIGIN      
        1 cttttttaaa aaaagtattc atcataaaag ccactacttg cggatcttca tcataaaatt
       61 ctgtagcagt acttgaaaag atgttccaat aaatatctaa tgctgcatat tgaatttgta
      121 cctcttttcc agaaaaagac tttctgccat tattagaaaa ggagatagac aaaccgtact
      181 atgctaaaaa tgcattaatc tttttcattt tcgtatacaa ataagaggca ctatagtttg
      241 tttgctgact gaaactgata atcgaatttg tctgacagct gaacaaagaa gtcaagatct
      301 gataactgac tgattcgttg cagtatcttc caaatagacg ataatagcag taagccggat
      361 caattccttc aaaattcaac tttaaaaaag gtttttgctt atcaatatgc agttctgtta
      421 gattgagacg ctcaaagtct tgttctaatt ctttgatgta tagtaaaaaa tttgattcag
      481 acatatggaa atgatctgct aaatattgtg tagtaataaa gaaacgatgt tcagatagga
      541 aaagaaaaat atctaatttt gtattgcttt ttttcttcaa gataaacgtt ttaatcgctt
      601 cttgagtttg catgaaagag gctccttttt acttaaattg ttaaaacttg aaaatattac
      661 tgtttttttc ataaatttag acaaaaaaac agtaaacata taggtagaat ctctactatc
      721 ttatttcttg catacaaaaa aatcaaggag tgagagagtt atgagatatt caaccatctt
      781 aatatcaaca tttgcagcct taagcttatc gcttactata aatcagacag tccacacaga
      841 ggagacagaa acagccaaat ggacagaaac caagtgggcc aacgtcaatc gaattacctt
      901 tcaggatgaa ttttcatcct tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc
      961 gaccatcaaa gcaaaaattg actattcggg agacttagca cctatactac aatcagctgt
     1021 tacatttgag aatgttgatc ccactttgtc gattggctat gacgagaaca ctttgactat
     1081 ccaacacaag caagcgatat ttaccttcac cgttacatta aatcaggact tgttaaaacc
     1141 agcgcttttt actgtcaagg tatcagatgg actaccgaac gatcatcatt taacgccgta
     1201 tagtcagcgg cagacaattg agcaagattc agccatcgac tcaggaggag atcttgttga
     1261 agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc
     1321 agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc
     1381 agagccagac aataaatcaa aacctgaaat aacccctgga gaaaaaccag atgtaactcc
     1441 agaaccagat acggattctg gaaaccaaac agtaccggaa acaaatccag acacagataa
     1501 tgaaactgaa aatccagaaa aaccagaggt agatccagaa gaaaaaccag atgtgactcc
     1561 agaaccagat acggacgcaa gagaccaagg aataccagaa aaaataaaca aaaaaacaat
     1621 tcaagaggat ggaaaaaaag agagtaaaaa atcaaatcta gcaattctaa aaataaatga
     1681 agagcagctg aataaaaaat cacgaatatt tgatagcgcc caatcagctg aaaccttaaa
     1741 aagtagcaag gatacaacct ttgctgtcct gaaacgaaaa ataaacagct accaaaatcc
     1801 ggagagtcac aaaacaaagt tatactgtgg agcggaatca ttctattaag tatagcaaca
     1861 atgctttctg caaaaagatt taaacaaaac agatctctct aacatttgaa taatgtctgt
     1921 gaacgcattt ttcaaataaa aagaaatatc cagaaaatga ccccaaaata ccagattgaa
     1981 atctgatatt tggggtcgtt catactttat ctttcttcat atttatttta agggatatct
     2041 gtttcttttg gaagatggtt cgggatcttt ttcactttca atacatctaa aaagaaggta
     2101 aagatcggaa tgccgacaat caagccccaa acgccaaaga accgttcgct aacaagtaag
     2161 accacaaatg tataaaaaat cggcagttct gttttgctgg acatcaactt cggattcagc
     2221 acataagact caattagatg gacaatcaaa atcgtaatca ggatataaac aacatctcgg
     2281 atgcctcctt gggaataagc tatgaaagat agaggaatac aagaaatgat aacacctgct
     2341 actgggatca gacttaaaat gaaaatcatg atcgctaaac tcagcagctg atgaaagcca
     2401 aatacagcca aggcgattgt agtgagcaac gtatttaaaa gagcgacaac aaattgagct
     2461 tctaatacca aaccaaatgt attgacaaac ttatcggcaa aatagtaaat atcttgaaag
     2521 aaccaagaaa attcactttt taaaaacaat cttgagaata caattgtttt tttcttttca
     2581 atcataaaaa agaaacttaa gatgaatgac atagcaaacg ccactgccat ttttcctata
     2641 tcctgtaaat agccaagcgc aatcgaagcg ccattttgaa gctgttcaaa aaaatcgttt
     2701 cgctcaatat aagtatggat atattgtaaa acaggatctg catcttttgg ctgatgctcg
     2761 taaaaatcaa taacggactg gatcatatca aatgtttgat gaataagcat cgggatatac
     2821 tttgtgaatg ccagatagat aaggtaaacg atcagtgtat aaatcatgat agctaaaaca
     2881 cctgtaggga ttcggacaaa tcgctgaatg atttgaacaa gatgaatcat caaataagta
     2941 aagataaatg tgagcaaaat ggttgtaatc atacttctaa ctaagaaaag aacaaaaacg
     3001 acaaataata aaacagtgag acggcgtatt ttttcattcg ccaaaaaacg ctgataaacg
     3061 gtcaaatcga caacctccaa ataacaaaaa ataaacacgt tattcattat atgatggaat
     3121 atgaaaataa gaaagagaaa gagcaaaaag aaaaaccgct aaatgacgtt atcttatgag
     3181 ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg gtcttgatga
     3241 aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc ctgttcaagt
     3301 ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa cgcagcacct
     3361 tgaggagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg acgattaata
     3421 taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc cagacacaaa
     3481 tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa catcacaaga
     3541 tactgggtat aggtaagatt cagcggcttg aggagggaag tgtagtgtct aatgatttcc
     3601 ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc ttccattttt
     3661 ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa gtctattcac
     3721 taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac ggacacactt
     3781 tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg atagatcctt
     3841 caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa acttgtgcac
     3901 tgacagtaga agccccagag atattttttt gctgtttgat caattctaat gcactgttaa
     3961 aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt tcgacgcgtt
     4021 taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgaact tctccagagc
     4081 gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta ccacctttaa
     4141 ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag aaatggattg
     4201 gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat agcaaacata
     4261 tacaaaaaaa tatcagagac aaaaactaat ctcatcataa acattacata tatttctcaa
     4321 aaagttgttt tctttcttca aaacttactt cacgatggac ctgatgaagc atccataaat
     4381 aaccaaaagg atctctgaac attgagttag tcacaccaaa gtccatcatt tctgtcattt
     4441 cttgaatctc tacacagcca tttcgtaatg ctttattatg tgtgtcctcg atatcagaga
     4501 cgatgatatt gaaccagatc gtatttggat gttcagcagt aggagcagtc aaaccaagag
     4561 acgtgttttc atccagcata tggaatctcg tgccatacaa ggtaaaaatg acttcattat
     4621 tgcctctagg gtaatctgtt gcctcaatca gttgtatttc atcaaagatt gtttgataca
     4681 gttctagtgc tttcaaacta tcatttaata caaaatctat ttccgttcct ttatgcataa
     4741 agatctcctc tccttttcag tcgaacagtt actagattct tttattatac cagttaaaga
     4801 aaacgcattc aaaaaaatag cggatttact taccaactca ttcgattgga tgataagtaa
     4861 atccgcgtcc cattacttct gctacgctgc tgacaatgac aaaggcactt ggatcgattt
     4921 cttctatcaa tggtttcatt cgggaaaatt catgatcagt cacaacgatc aataaaacct
     4981 cttttgactt tcgattatac ccgccttttg cctctagcag ggtaaggcca cgtccaatct
     5041 cagcacttaa gcgaaggcgg atgtcttcca aataatgttc actcatgacc ataacagatt
     5101 tctttcgatt gatacctgtt tcgatataag tcatgacaat tgaagtgatt gcaatagata
     5161 aaatagcata aagaaactct tctattccaa aaacaaacag attaagagaa acaacgaatg
     5221 cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa atatttttta aaaatgaggg
     5281 gagggatcgt cgtaccgccg ctagaagagt tgtttttata taaaatcgca actcctaaag
     5341 caaaaatcgc acttccaaaa atgacagaca acaaccgatc tgaagtaatc attgtttctg
     5401 gaactattgc aatgattaaa ggaaaaaaca agcttccgaa aagtacttta aaaaagggtt
     5461 ttttacctaa aaatagaaga gctagtacca acataacgat attcaacact aaaataacta
     5521 tagctcggtc aaatcctaaa acagcttcca gtaatattcc tagaccgctg acacctccag
     5581 cagcaatatg atgaggccct aaaaacatat tgatagcgac tcctaatatg aagagggcaa
     5641 aaataatctt aatgtatgtc catataaatt gtctcgtttc ttt
//
LOCUS       DAZKCY010000137         5554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000137 DAZKCY010000000
VERSION     DAZKCY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.138"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..548)
                     /locus_tag="ACG8XC_002446"
     CDS             complement(<1..548)
                     /locus_tag="ACG8XC_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFP9192113.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEF"
     gene            complement(553..738)
                     /locus_tag="ACG8XC_002447"
     CDS             complement(553..738)
                     /locus_tag="ACG8XC_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP9192114.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(1092..1337)
                     /locus_tag="ACG8XC_002448"
     CDS             complement(1092..1337)
                     /locus_tag="ACG8XC_002448"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9192115.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(1331..1732)
                     /locus_tag="ACG8XC_002449"
     CDS             complement(1331..1732)
                     /locus_tag="ACG8XC_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9192116.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1918..3726)
                     /gene="uidA"
                     /locus_tag="ACG8XC_002450"
     CDS             complement(1918..3726)
                     /gene="uidA"
                     /locus_tag="ACG8XC_002450"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFP9192117.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(3749..5515)
                     /locus_tag="ACG8XC_002451"
     CDS             complement(3749..5515)
                     /locus_tag="ACG8XC_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFP9192118.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT         1738 a         1091 c          697 g         2028 t
ORIGIN      
        1 caaaattcta cttgaggacg ctgagttttt attttaccta tagtatctac aggattttct
       61 tcaataattt caagtacaat tgccctttta aaaactactc tcaacttatt gaatatctgt
      121 ttaatataat ttggtgataa tggttgctca atgccctttt ttacaacatg gattttcgat
      181 aaaaactgtt gaaattcctg tatatgaatt ggtcgaatct ttacaacctg catttctcca
      241 aaaaaatcca atgcacgttg taaaatatga tctgttttca tatatgtttt ttctacagtt
      301 cccaacttat accaaggaaa aaaatactct cgataaaaat catgaaacgt tattccttgc
      361 agataaattc cttgtgaatc agaaaaactt tgttttaact cttcgagtcc ccttctagct
      421 tctccttgag ttttgaagcc tcttctggtc acttgaattc tttttccaaa gcgatcttgt
      481 cctaaagttg cagaaaaaaa ccaagtgcct ctttttctgt ctttataaac attcgcaatt
      541 ttcctcattt tactattcct cccaagaaat atggagaact tcttctaaaa ttgaaatagg
      601 aattcttttt atttttgaat tacgataaaa aaagtatcct ttctcaacta aaagttcttt
      661 acattccttg aagattttat tagcatagta ctgagaaaaa cctagattaa tcaaatcttt
      721 tcgagtagct gttttcatta tagcaccttc cttacttttt ttcatttatt tctatcagct
      781 gcttttgtca aaagcacata ggaatttcac ctctccccaa gtttacggcg agccttacgg
      841 aagtatcatt atcccaactt taaatcatcg ctttttattg actataaaaa ttattttaga
      901 atatttatcg ctccttcttt acagcaagaa atcatggcgc aactaaaata tgctccttaa
      961 agtgttaacg tactgataga atctatttaa ttttcaatga acaataggtt ttctacctaa
     1021 aagaatctac taattcctta actattagaa gattctttta aacagaaaaa gtggaaaggg
     1081 gattttttta attagttaat tttaaatttt aagatcgctt ctattaattt aatttctagc
     1141 tgacttatca tatattcatc tacataggta acgaattgac ctacctcgtt aaatccattt
     1201 gtcaaacata acttagccat ataagaatga taatgtttga gtatcatctt gatcgaaatt
     1261 ggatctcctt ctagagcaga taagataatg gatagtggaa ctaataggta ctgccgcttt
     1321 ttagatttat ctaccatagg tcatacccca ttttcaattg ttcaactgcc ttctttcgtt
     1381 gataccagat accacttgtg ctcatattga gaattttacc gatttcagca tcattaaaac
     1441 ctgcaaaata ataaagtagg ataatctttc ttcttatctc cgataactga tcaattgctg
     1501 ctgataactt ttgatctgaa attaatattt ccattccaaa taagaaatag acatcttgtt
     1561 catcaaaatg agtttgggaa tacctgcctt catttgaatc tgccaatggt tcctcattat
     1621 cctttatgtt agcgtttcgt tttcgaatag acttagcttc gtttcgtaat acttttttac
     1681 agtaggcatc aaattggtgt tgtatagata tgtcattgta atgttgttgc atgataatat
     1741 gctccttttt ccccctttcc actaatacaa caatcagaaa gccccttttt ttcgaaaaaa
     1801 ctagaaaaaa aacataaaaa tatttatcat tctttaaaca gaaaatcttt ctataaaaaa
     1861 caaaaaaaac gattaggcta ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta
     1921 ttttttataa ctgaaattgg gtattgatga ccatcgtttc tgtagatagt aagcaatcat
     1981 tttaggtttt ctatctctag taaagattcc tttcttattt ccttgcactc ttaaaatccc
     2041 ttgacttgtt gcaaaatctg caaaattcca cacttgttca ccaacaaaat tttcacatgt
     2101 atctattact tcgtgatttg cttcgtaata agccacttga tactcttctg taaacattgt
     2161 tggtactgta tcatgaaatc cagctactgt atctgctcca tattctgtaa acattattgg
     2221 cgtatcagga catctttcca tccatccatc tagctctttt ttcaacagat attttgcatg
     2281 tgccagatcc cctcccgaaa cataccaacc ataataacga tttaaacata aaacatcgac
     2341 aatatctcct accttacaaa catctggcgt tgattttaga taagtaacga ttgttacagg
     2401 tcttttctgc ggatcaagtt ctttagttaa ttcaactaaa ggttcaaaat attctttagc
     2461 accttcggaa tccgaatctg gttcatttgc aatagaccac attacaacac ttgggtggtt
     2521 cttgtcgcgt tctatcaatt ccttgatcac cgttttatga gaattttctg ttcgtatctc
     2581 tttccacgta ttatgttttt ccacattatc atctaataaa gtggccatga aatttaaatg
     2641 taaccctact gcaggtactt catcaataac tacaatacct tctcgatctg ctaatcgcat
     2701 aatttcttct gagtaaggat aatgtgatgt tctaaaagaa ttagcaccta tccatttcat
     2761 taaattaaaa tctttaatat ttacagcctc attcaaacca cgtccattaa catacgaatc
     2821 ctcatgctta ccaaatcctt taaaataaaa aggtttatta ttaatcaaaa atttgccatt
     2881 ggtaacctga acactgcgaa taccaaattc ttctgtatag gtatcgatca ctttattatc
     2941 atcaattaaa ctaattttca tactatataa ataagcgtct agaggttccc aaaaaattgg
     3001 attcttaagc tgaatcattc ctttctttcc ttcagcttca gctacaagtt gagcctcttc
     3061 gtcatatatc gctattaaga tttttccggt ccattctgta tcgatattga attctacact
     3121 tgcatccaaa tcagttattc ttgtattaat agtaatatca cttatatagt ctttcggagt
     3181 tgtatataac ttaacaggtc tatgaatacc tgcataattg aagaaatcaa aattaactaa
     3241 gttttccttt tttatatccc cattttcctg tactgtttcc ttcataatcc ccacaggtaa
     3301 agtactatcg tcaataatat tgtttacagc aactgtaatt cgattattcc cgtcttttac
     3361 aaaccgatta atttcagact caaatggtgt aaacccacct ttgtgttgag taataaactt
     3421 tccattcaaa aaaacttttg cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc
     3481 ttctaataat cttttaggaa gagtcacttc tttttcatac cacacccatc caacatgatt
     3541 tctaatgttc tctgtttcaa ataaatcatt ataagaagag ggaactgaaa tcggataact
     3601 ctctttttca tttattggtt ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa
     3661 tttaaatctc cagagaccgc ttaagtcaat tacttgcctc gtctctgtca taatgggata
     3721 caacatataa accactccta tctaaatatt aaataaatgt atcctttaaa ccacaataag
     3781 catctattgg acttactccc ttaaattcag aaagacccat tagtttgtcc accaatattt
     3841 ctaaagtttc ttgttttcca tcataagtat taatataagt ctttacttga ggtacatcag
     3901 ccaaatgaaa cggacactgt acagatacaa aaattgttgg aatttcatga atgtaaaacg
     3961 gtatatccgg agttccttta cttgctggcc agacaattcg ttgaactgtg cctggattaa
     4021 cattagctat attaatgatc aagtcatatt ttttagttaa atttttaatt ggtctcttag
     4081 cagcatatat tccagctata tttttaattt tttcttcttg cgataatcct ttcaattctt
     4141 cttcaggtga aatgtatttt tcaacttcaa atccttttgc tgacaaaatc tgtttcaata
     4201 aatcaatggc ttgaggtcta tttccagcaa ctacttcacc aaacccacct tttaccccat
     4261 gtacatctac tagtaaaatc cttttatatt tttctggtac aataggccaa atatcttgtt
     4321 tatctttaac taatgtaatt gcttggtcag ccacctcttt aaaaattgat ttattttcaa
     4381 ttaaaccaat tttttccaga gcttcttgct taggtaataa aatttgttct tttgcttttt
     4441 tgtgtaatcc caatttagct tttaacccta aaattctttt taaggcatct aataaccgat
     4501 catcagtgat aattccttta cgatagcctt ccatcatata attaaagtct tcatctggat
     4561 cattaaaaaa tagaaataga tcacatccag ctgcaataga agtgggcaat aattctgatc
     4621 gtttcattgc cccagtcatt cctaacatat gactagcatc agtaactact aatccattaa
     4681 atccgagttt tcctctaagt aagtcagtga ttaattcttt tgataaagtt gcaggaagta
     4741 aatcttcatc tttcagatca ggattaaaat gttttgaata agctggcata tggatatgac
     4801 ctgccataat tgaaggtaat ccagcttcta ttaagcctga gtatactttt ccaaaagaat
     4861 tatcccattc ttccgtacct aaactattaa tactaaaaga tagatgctga tctcgttcgt
     4921 caataccgtc accagggaaa tgtttagctg caggcaaaat tccactctcc tgaatacctt
     4981 tcatataagc taaactaagt tctaaaactt tatcagggtt ttgtccaaaa gatcgactag
     5041 aaatgatagg atttctccaa tttctattaa tatctacaat aggagcaaaa ctccagttac
     5101 atccaatagc cgatgcttca atcccagaaa ctctccctaa ttcataagca tatttcgcat
     5161 catttgttgc accaattttt acttctacac caacttcagt accatctgta caagctccgt
     5221 taccacctgc ttccgtatta gcagcaatta atagaggaat tttactttct gtttgtaaga
     5281 tatagttttg atcatatacc tcatcagcag ttcctggatt atatcgtaca gctccaatat
     5341 ggtactgatt gaccatatta ctcaagtaat ttttatcacg actagatccc atattaacaa
     5401 acaattgacc tattttttct tccaaagtca tagaatcaat cgtctcttca atccaagaaa
     5461 tagcatcttc atctaaatta tatggttttt ttcgtaaatc tacttttata gtcattttct
     5521 ttctcctcta cttattagta agatttttcc aaaa
//
LOCUS       DAZKCY010000138         5401 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000138 DAZKCY010000000
VERSION     DAZKCY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.139"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..447)
                     /locus_tag="ACG8XC_002452"
                     /pseudo
     CDS             complement(334..447)
                     /locus_tag="ACG8XC_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(830..2062)
                     /locus_tag="ACG8XC_002453"
     CDS             complement(830..2062)
                     /locus_tag="ACG8XC_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9192119.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2316..2885
                     /locus_tag="ACG8XC_002454"
     CDS             2316..2885
                     /locus_tag="ACG8XC_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9192120.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3063..3503)
                     /locus_tag="ACG8XC_002455"
     CDS             complement(3063..3503)
                     /locus_tag="ACG8XC_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP9192121.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3661..4425
                     /gene="map"
                     /locus_tag="ACG8XC_002456"
     CDS             3661..4425
                     /gene="map"
                     /locus_tag="ACG8XC_002456"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP9192122.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4457..5380
                     /locus_tag="ACG8XC_002457"
     CDS             4457..5380
                     /locus_tag="ACG8XC_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFP9192123.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT         1678 a          981 c         1043 g         1699 t
ORIGIN      
        1 ctttataaaa atctttgata aatctattcg aagggtcaaa tttaaaccat aattgtgttt
       61 ttccttccat cagcataaaa gttcacctca aaaagagtat acgaacgaat gttctttttg
      121 tgaaggcgga atttaatata ctgaataata gaaaacaagt acttgtgcca aattatgtgc
      181 caaaaaaatt cgaattcaac aaaatgtaaa caaatagaaa accttctata ttaagatttc
      241 ctgcaataac aaacacaaca gaagacgtgt aatagttagt caggaacgta caaataaccc
      301 ctgtatcctt tgcggtacag ggggttattt tgtataagta gacattgtag tttctaaact
      361 gctatgtcct aaacgtttcg atacgctaag tatatttact ccttgataaa gtaaaataga
      421 tgcatgcgta tgtcttagtc catcaataaa cagtcgaaga gaaaataggg aaaaattgat
      481 tactaaagaa aaacttacta ggtcgacggt gtagttgatt ttcatacttc aatagattcg
      541 aattaaacga tttcgattgg cttataccag tcctatccaa cgaaagatat atcgttctta
      601 actctagcgg tataacttga agctttacgt aaagccaatg ggaatgaact ggagatgact
      661 ttttattatt tttagtaata gctgatgggt taatacttac aactttcctt ttcaaagtac
      721 catataaaaa aacggtgggg ttgtttagcg aaataaagga tctaagccaa tagcttagat
      781 cctttattct atgctttata ttattttttg catgtgtgcg aaagtatact caagcccaat
      841 cgccatttcg gaaaatcggt actgtagagc catcttcacg gattccgtcg atatccatct
      901 tgtcagatcc taccatgaaa tctacgtggg tttgacttct attcaatcca gcttctgcta
      961 attcttcatc ggacatttct gtaccgcctt tcacactgaa ggcataagca gaacctagtg
     1021 ctaagtgatt cgatgcgttt tcatcaaata atgtattgaa aaagatgatg ccagattgag
     1081 aaataggtga agggtcaggt acaagtgcga cttctcctaa acgacgcgca ccttcatctg
     1141 tatcaagcag ttttgctaag acatcttctc cttgttcggc agaaaaatca acaacttttc
     1201 catctttaaa agtaaatttc atacccgaga tgatggtgcc agcataactc agtggctttg
     1261 tacttgaaat gtaaccatct actcgatgac tgtcaggtgc tgtgaacact tcttccgtcg
     1321 gcatatttgc catgaatttt tctccacgag cattatagct tccagcgcct tcccaaagat
     1381 ggtttttagg caatccgata atgatatctg taccgggagc agtatagtga agggcagaaa
     1441 attgttctcg attcaattct tctgcttttt ttgctaattt ttcatcatgc ttcttccatg
     1501 ctaagacagg gtcttcttca tagacacgag tcgttttgaa aatctgatcc cataatgcct
     1561 caacttgttt ttcttctggc agttctggga agacttttgc tgcccattgt ttaccagccg
     1621 cagccacgac agtccaactg actttatttg cttgagttgc cttgcgtaaa ttcattagtg
     1681 ctttgccatt cgctgattga taagacgcta ctcgatggct atctacacca gcaaatgcat
     1741 cgggatcggc agaaacaacg ctgatcctgc ttgctccttt ttcaagccat tcatccgctt
     1801 gatcgatttt agattgtggt acattctcaa tacgatccgt tgcagcatgt aaaagaaatt
     1861 ctctttggat ctggtcgtca gtccattgaa cgataacctc agcggctcct agtttataag
     1921 cttcttgtgt gatcaatcga gcaaggggtg cctgttccac gctgatctgt aaaacaactg
     1981 tgtgtccttt ttctgtggct acgccagttt ctgcaattaa gcgtgcatat ttttttagaa
     2041 gttcattaaa atcagataac ataataaaaa ttcctcctca ccattttttc tggtaactat
     2101 tagcatgata ccatttaatt atgattcaaa caatgaggct ttaaataaaa aaaggaggtt
     2161 atttttttaa ggttaagttt catgatatat gttactactt gctatttcag cttgtgaaga
     2221 aaatcaaaaa ctaattttgc atatcaaaaa aacatgttac aatgaaatta atcaatggtg
     2281 gaagcgttat aaaaaaatta aggagattaa aaaatatggc aagaaaaaag acgattacta
     2341 gagaacagat tttggcagct gcttatgaag tagctgccac cgatggattt acacgattta
     2401 ctgcgagaaa catcgcgaac aaaatgaatt gttccacgca accgatttat cttgagttta
     2461 aaaatatgga tgacttgaga caagctcttt ttgagcagat ctatgatcat ttacaatatg
     2521 aagtgttcac tgttgagcat acagggaata cgattatcga tcttgctttt aattatatcc
     2581 attttgcaag aagagagaag aagttgtatc gttcactgta cttagaagaa tatggtggag
     2641 gaaaagagat gcagaatttc tcttatactt actttgtgaa tacagtgaaa cgcgatccag
     2701 cttatgtgaa tctttcagaa gagcagatcg aatctttgta taatgggaca tggatcatag
     2761 ctactgggat cgcagctttg atgtcttcaa gcatcatcca tccaactgat gaacagattg
     2821 aaaagatgat ccaagacagt atagatgcta tcttgaaatt caaagaacca atcgaaatcg
     2881 attgaatata ctcaaaagtt ccacaaatca gtaggtaaca agagatgtgt caaaataagg
     2941 aaaagttcgt ttttcttaca gcgtatgcat ttttaactcc cgaagaactc ttccatgaat
     3001 gcaatgaata aaagagccat acagattgaa taaatctgga tggctctttt attgcaagta
     3061 tattaaattt ttgcgactag ttctttagcg aaagcttcta aattttcgat atcttcttct
     3121 tcagcattca gatccacttt aacattttcg gctccttttg atgcgcctgt ttttgcaaaa
     3181 gcagcatcga aatcatccac cgatttgcag aattcatcgt aaaaagtatc accagatccg
     3241 cagacaccgt aaatttttcc caatagatcc aattcttgca agtcttcata aaagtctacg
     3301 atctcatcag gcagatcgcc atcatcgtat gtataagtag ctactacgca aatatctgcc
     3361 tcctcgaatt catctgcatc tacttgggta cattcgttga tttcgacttc gatatccaga
     3421 ttttcaaatg cttctgcaac aatatcggcg atttcttcag tgtttcctgt catgctagca
     3481 tatacgattt ttgctaaagc catgatgtgc ctcctcgttt ataattattt aacaattatg
     3541 aattatacca aaacatcatt gaaaagaaaa taaaacctaa agacacctgg ggaatgtcat
     3601 ttggctaaat gtatgccgct atgttaaaat gagagggatt tgtaaaaagg agacgaaaat
     3661 atgattactt taaaatcagc acgtgaaatt gaaatgatgg cagaatctgg agctttatta
     3721 gcagatgtac ataaaaatct gcgtgatttt ataaaaccag gaatcacaag ttgggatatt
     3781 gaggtatttg tcagaaacta tatcgagagt catggcggaa ttgctgcgca aattggcttt
     3841 gaagggtatg aatatgcgac ttgtatcagt ataaatgatg aaatttgtca tggattccct
     3901 cgtaaaaagc ctttgaaaaa cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa
     3961 ggcggcattt ctgattcttg ttgggcctat gtagttggag aatctacacc tgagatcgac
     4021 cgattgatgg aagtaacgaa gaaagctttg tacatcggta ttgagcaagc acaagtaggg
     4081 aatcggattg gtgacatcgg tcatgctatc caaacttatg tagaaggcga aaatctagcg
     4141 atcgttcgtg attttattgg tcatggcgtt ggtcctacga ttcatgaaga accagcagtt
     4201 cctcattacg gagaagctgg aaaagggttg cggttgaaag aaggcatggt tatcacgatc
     4261 gaacctatgg ttaataccgg aacttggaaa atgaaaatgg atcctaacgg ctggactgcg
     4321 tatacaagag acggcggact ttcttgccaa tatgaacata cattggctat tactaaagat
     4381 ggtccaagaa ttttgacatc acaaggagaa gaaggcactt attgatctat ccagtcaata
     4441 ggtacgggag aggtacatga aagtttggaa caaaataaaa caaaatgaaa atctgatgcg
     4501 ctttattgag acgactcaaa agcgcatcac tgattcagaa atgagtacga cctctgtcgt
     4561 cgttgcttat tatttactct tgtctttatt tccattgatt attgcttttg gtaatatttt
     4621 accgtttctc cacatcgatc aagagactgt actgacctat ttacgagaag tgattcctga
     4681 gaccgtttac cagtttatcg gtcctgcaat caaagacctt ttgacacaaa gttcaggagg
     4741 cttgttatcc gtttctgcat tagcggcttt atggtctgca agtcaaagta tcaatgcttt
     4801 gcaaattgcg atgaataaag cgtatggcgt agaaaataga aaaaattttt tgatcgtccg
     4861 tttcttctca ctaattgtca ttgtcttgtt catgattgct attagtgggg taactatcgt
     4921 tcttgggttg ggacagctga ttttggatgc tattcagcca atcgtacaaa tacccgtgaa
     4981 ctttatcgat cagttccaaa ctttgaagtg gccaatcaca ttggtagctt tatttgtcat
     5041 catgttcttg atttatttaa ttgtccctaa tgctcagttg aagttgaaga cgatcattcc
     5101 gggcacgatc ttcgctactg tcggctggat gctgttatca caagtgttta gtatctatgc
     5161 taggtatttt gcaacgagga tcagcggtta tcagatcata ggaagtttta ttgtactcat
     5221 gctttggctt aattttgctg ctacgatcat cattttgggc ggaatcatca atgcagttgt
     5281 tcaagaatac gtgacgggtc atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt
     5341 caataaattg aaaactaaat tttctcataa aaaaaagtag aaacaagagt tcttattgta
     5401 a
//
LOCUS       DAZKCY010000139         5362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000139 DAZKCY010000000
VERSION     DAZKCY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.140"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..358
                     /locus_tag="ACG8XC_002458"
     CDS             2..358
                     /locus_tag="ACG8XC_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFP9192124.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            374..724
                     /locus_tag="ACG8XC_002459"
     CDS             374..724
                     /locus_tag="ACG8XC_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFP9192125.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            753..1532
                     /locus_tag="ACG8XC_002460"
     CDS             753..1532
                     /locus_tag="ACG8XC_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFP9192126.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1543..2220
                     /locus_tag="ACG8XC_002461"
     CDS             1543..2220
                     /locus_tag="ACG8XC_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFP9192127.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2239..3345
                     /locus_tag="ACG8XC_002462"
     CDS             2239..3345
                     /locus_tag="ACG8XC_002462"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFP9192128.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3335..4426
                     /locus_tag="ACG8XC_002463"
     CDS             3335..4426
                     /locus_tag="ACG8XC_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFP9192129.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4439..5179
                     /locus_tag="ACG8XC_002464"
     CDS             4439..5179
                     /locus_tag="ACG8XC_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFP9192130.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1724 a          873 c         1268 g         1497 t
ORIGIN      
        1 catgattaaa gtagttattg cagaccgtat gggtaaagga caaaatgtgg ccaaaggggt
       61 agaagcagct ggtggaaaag cagttgtcgt accaggaatg ggggcagata tgcgtttggg
      121 cgatgtgatg caacaagagc atgcagatat gggtatctct ttttgtggta gtggtggtgc
      181 aggtgcatta acagcagcaa caaaatatgg ctatccagaa cgacatggta tgcgttcgat
      241 tgaagaaggg atcacagcta tcaaggatgg caaaactgtt ctaggtttcg gctttatgga
      301 tcaagaggaa ttagggaaac gtctgacaga agaatttatc aaaaagcatg gaaaatagaa
      361 agagggacat gaaatgagcg ttcaaaagac aattcagcaa aaagttatcg tctccggtaa
      421 aggggaaaca aagcaacatg cttttgcatc agcgttagca gaaattcaaa acaaaataat
      481 ggaacaatca aacgtattat tgcgaatcga accagtcaat gtggatattt taaaggcggt
      541 cgaaaacaaa tacacagaac gctttctatt tttcttcttc ccacggcaac gtattagcta
      601 tgaagtcgaa ctctctgtta ctgtggctgt aacagaaata aaattggcag acatatcgtt
      661 cagccagcaa atgactgaag atccaaatgg gattgccatt ccatttgtaa ctaaaaaaat
      721 ctaattttga atcataaggg ggaaagaata acgtggattt cgtaatcatt ctagtcaaat
      781 cggtcattat cgggggatta cttggttttg cagcatctgt cggggcagct cgaatgtttc
      841 atgcacctaa tacacaaggt ttaggagctt ttagaacatt aggcgaaatg aatgcctgca
      901 tgggtgatcc agcttctcat ttttcatttg gtttaggctt tttctttaat gcatgggctt
      961 cttcagttgg tgctggtgca ttcacacaag atgtgacgca tcgaatcatt cccaattggg
     1021 cagcggcagc acttctagca aaaaacaaag atgtcaccca gaccatgcat gatccgaaaa
     1081 aaatggggat cattggagcg gttatcgggg cagtggtggt agccttctta aacacaacag
     1141 ctgcagcaat tccagaggcg ttacaagtga cggcggtttc cgttttggta ccagcagcga
     1201 cgattctgat caatacagta atgccagtta ttttttggtt ggcagcttta gacgcaggga
     1261 aacgaactgg tttttggggc actctttttg gtggtatcgc gcaattgatt atgggaaatg
     1321 cggtacctgg tgttgttcta ggaatcttga ttggtaaagg cgtcgatgag agcggttgga
     1381 atcgcgtcac gaagagcatg atgatcgcta ttgttttatt atttattttc agtggatttt
     1441 tccgtggatt tgatatggaa atgatcaaga gcttccagct tcaagtgcct aattggcttg
     1501 ataacttgca caacatcttt agtgtgaaat aggaggagaa tcatggagac aacagaagaa
     1561 caagcaaaag tattagaagc aaagcggcaa aagagctttt ggtttgcaga ctggtctttt
     1621 ccgctaatcg taggaattat gtccgctgct gtatttgcag gaacacacat gtatgtgatg
     1681 tatggtgtgg gtgcattcaa tgaagtttcg atcgtggcga tgttaaaagc cgggctggat
     1741 ggcggatcct atggcgcagc ggcagcgttt ggtgctagtt tcttatttgc cagaatttta
     1801 gaaggctctt tagttgggat cttggactta ggcggttcga tcctaactgg gatcggaatt
     1861 ggggtaccag caattctctt aagtatgaaa atagcggcac caatcgataa ttttgcactc
     1921 tctttattga ctggtttggt tctaggtttg attgttggtg gcatcattgt cttgattcgt
     1981 aaatttacga tcaatcaatc aaattccact tttggtgctg acgtcatgat gggggcagga
     2041 aattcatctg gacgtttttt gggtccatta attatattaa gcgcgtgtgc agcatctatt
     2101 ccaatcggca ttggcgctac attaggagca ctaggattct atgcttggaa aaaaccaatt
     2161 gctggaggtg ctattttagg agcaatgatt ttaggagcaa tcttcccggc agtaaattaa
     2221 tagaaagagg gtcattaaat gtttgatact ttgattaaaa atggaaggtt agttgatgga
     2281 tctaaaatag aggtcgctat agaaaaaggc atgattaaag cagttgcagc agaaataaat
     2341 cagccggcgc aagaagtagt tgacttggag ggaaagtatt atttatccgc tgggtggatc
     2401 gatgatcatg tccactgcta tgaaaagatg aatctttatt atgactatcc tgatcagata
     2461 ggggtcgaaa aaggtgtgac gacggtcatt gatgctggaa ctacgggagc agaaaacatc
     2521 cgtgaatttt atcaattgac gaaatcggtc aagacaaatg tgtatgcgct aatgaatatc
     2581 tctaaatggg gcattgtcaa acaagatgaa cttgctgatc taacaaagat tcaagaagat
     2641 ctagtaagta ctgcgttagc agaactgccg gaatttattg tcgggattaa agcgagaatg
     2701 agtaagacag ttgttggtga aaatggcatt aagccattag aattagctaa aaaaatccag
     2761 acagaaaaca atgatctgcc attgatggtt catatcggtt ctgcacctcc tgagttagaa
     2821 gaagtattgt caagaatgga aagaggcgat gtattgaccc attgttttaa tggaaaacca
     2881 aacggtattt tagatccaaa gacagatcaa attaaaaact ttgtctggga tgcttacaat
     2941 aatggtgtcg tctttgatat tggacatggg acggatagct ttaatttcca tgtggcagaa
     3001 accgcactaa aagcaggaat gaaagctacc tctattagta cggatatcta catccgcaat
     3061 cgtacaaatg gcccagtttt tgatctagca actactttag aaaaattgag ggtagtcggc
     3121 tattcttggg aagaaatcat tgaaaaagtt acagcagttc ctgcacggaa ttttcatttg
     3181 aagaataagg gccagttagc tgtcaattat gatgcagatt tgacgatatt tgagattgtt
     3241 gaaaatgaaa aggaattgaa agattccaac ggatttacac gtatagctaa agaacaaatt
     3301 ttaccgatta aaacaattat tggaggcgtc atatatgaca attagctatg gaaagtttga
     3361 tttaaaagaa gtcattaatg catcgggaaa aatgacgatc ctaggggttt ctaaggtatc
     3421 tgaaagtgtc ctagcagcac aacgatttgg aggcgagcat ttttttgaga tgagtgatct
     3481 tgccatcaaa accggcgctt atctttctca attactgaaa gtggaagatg cgcaaatcgt
     3541 ttcttctgct tcagcaggga ttgctcaatc tgttgctgct ttaatcggac agggcgacat
     3601 gtaccatgtc tatcatccct atacagaaaa aatcgctaga cgggaaatca tcatacctaa
     3661 gggacataat gtagattatg ggacaccggt tgaagtaatg gttgctcaag gtggaggaaa
     3721 agtcatagaa gctggctatg caaatatgtg tacaccggaa catgtttcca tgatgattac
     3781 ggaaaaaact gctgcgcttc tatatattaa aagtcatcat gcagttcaga aaagtatgct
     3841 gacggtggca gaaatggtag aagttgccaa agctcatcgt ttgccattga tcgttgatgc
     3901 agctgcggaa gaagatttat tcaaatatag cgaaatgggc gttgacttag tgatttactc
     3961 tggggcaaaa gcaatcgaag gtccgagtgc cggactggtc ataggaaaaa agaagtatat
     4021 tcaatggatt cggctacagt ctaaaggtat tggccgagca atgaaaatcg gtaaagataa
     4081 tattttaggc tttacccagg cactagaaga ttatttaaaa aatgggagcg aatccggtga
     4141 ctccatgaag caacgattag cgccttttgt taacgcaatc aatcaacttc ctgacctttc
     4201 agcgaaaata gttcaagatg gtgccggcag agatatttat cgagcaagtg taacgattaa
     4261 tggcgataaa agtgcgaaag aagtcattca ggaattaaaa gcacgaaatc cagcagtcta
     4321 cacccgagaa tatcaagcaa ataacggaat cattgagttc gacattcgtt ccgtcagcca
     4381 aaacgaaatg gaaaaaatta taaaaagatt gagagaaatc atgtaggagg cttttaaatt
     4441 gtcagttatc ccaaattatt ataaaaatcg tgtatgttta aatgtattag ctcattcagt
     4501 tgaaaatgca aaagcatgct atgaagcagc ggaagggcat gtcgtactag gtgtgctatc
     4561 gaaaaattat gctagcgatg aagcagcaat cgaagatatg aaaaggtacc aagacgcaac
     4621 caataatgcg ttgtcagttg gattaggcgc tggagatcca aaccaaagtc agatggtttc
     4681 gcgtttatct ggggttttac aaccacaaca tgtcaatcaa gtatttactg gcgtggggac
     4741 ttctcgtgct ttattgggcc aaaatgatac agtcatcaat ggattggttt caccaacagg
     4801 taagatcggc tatgtcaata ttgctacagg tccgttaagt tcacaagcgc cagcagctga
     4861 agtgcctatt gaaactgcca ttcaaatgct gcaagacatg ggaggtactt caatcaagta
     4921 tttcccgatg aaaggcttag cacataggga agaatttaaa gcagttgcgg aagcttgcgc
     4981 aaaatatgaa ttctatttag agccaacagg aggaattgat ctttcaaact ttgaagaaat
     5041 tctgaaaatt gcaatcgatg caggtgtaaa aaaagtgatt cctcatgttt atagttcaat
     5101 tatagataaa gaaacaggag atacgcgtat aggagacgta aagatactat taagcatggt
     5161 aaaaaaaatt attcactaac tcattagaaa ataagaagct tattccagaa tgaaattaaa
     5221 tggaataagc tttttataat taattacgga aattgttaat gtagataata atttagatgt
     5281 acgtaagcag aagtacagaa atgtaagatt ttccaatatt attttattca acataataaa
     5341 tcgtatatca atttttttag cg
//
LOCUS       DAZKCY010000140         5199 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000140 DAZKCY010000000
VERSION     DAZKCY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.141"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..696
                     /locus_tag="ACG8XC_002465"
     CDS             25..696
                     /locus_tag="ACG8XC_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9192131.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACG8XC_002466"
     CDS             755..1195
                     /locus_tag="ACG8XC_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192132.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACG8XC_002467"
     CDS             1207..1875
                     /locus_tag="ACG8XC_002467"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9192133.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACG8XC_002468"
     CDS             1939..2562
                     /locus_tag="ACG8XC_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFP9192134.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACG8XC_002469"
     CDS             2618..3004
                     /locus_tag="ACG8XC_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFP9192135.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACG8XC_002470"
     CDS             3217..4608
                     /locus_tag="ACG8XC_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFP9192136.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1728 a          861 c         1095 g         1515 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacag
//
LOCUS       DAZKCY010000141         5197 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000141 DAZKCY010000000
VERSION     DAZKCY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.142"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..923
                     /locus_tag="ACG8XC_002471"
     CDS             120..923
                     /locus_tag="ACG8XC_002471"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9192137.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            956..1810
                     /locus_tag="ACG8XC_002472"
     CDS             956..1810
                     /locus_tag="ACG8XC_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HFP9192138.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            1807..2508
                     /locus_tag="ACG8XC_002473"
     CDS             1807..2508
                     /locus_tag="ACG8XC_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFP9192139.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            2516..3583
                     /locus_tag="ACG8XC_002474"
     CDS             2516..3583
                     /locus_tag="ACG8XC_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9192140.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            3576..4541
                     /locus_tag="ACG8XC_002475"
     CDS             3576..4541
                     /locus_tag="ACG8XC_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9192141.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTFEDAYIIIDLLFQTEKIVVSNEEKYFYLQRNDSIMGKSFSM
                     NNFDVIEAWQYNKEKILDAFPDLHDSYYRRLCWAYFYVLDKMVLSSSYQQIPRKKTVI
                     EFLKKNRNFILTYKGFTKSRKIAIIALSFRVDWYRKLVEQKNKKN"
     gene            4586..>5197
                     /locus_tag="ACG8XC_002476"
     CDS             4586..>5197
                     /locus_tag="ACG8XC_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9192142.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYY"
BASE COUNT         1914 a          703 c         1012 g         1568 t
ORIGIN      
        1 agatgaaaat attagttcat tagcagaaat cgacttttca caagagaaaa tttatcgcta
       61 gattggaaaa attttgagca aagaggtagg agagaacatt ggaaaataaa aatgtaagta
      121 tgctgctttc tttatatgat aaagaaaagc cagagtattt aaaggaagca ttaactagta
      181 tttttgagca aacagtaatg ccaaaagaaa ttgtattagt ttatgacggc cctataaatg
      241 aaaaacttca agtagttgtc acttatttcc aacaaaaaga tcctcacttg tttacagttg
      301 taaaattaga aaaaaatcaa ggattgggga ttgctttagc aattggatta gaacatacga
      361 aaaatcgctt agtagccaga atggatacag atgatataat ggaaccccaa cgattagaaa
      421 aacaattaga gatcttccaa aaagatccag aaatagcgat tgttgggagt aatattgagg
      481 aatttactgg cacgcttagc aatgttatcg gaaaaagaat tgtacctgaa tataacaatg
      541 caattcgtgt attttctaaa agaagaaatc cctttaatca tatgaccgtt atgtttgata
      601 aaaaagctat tttggaagtg ggaaattacc aaccattaag tgggtttgaa gattattatc
      661 tttgggcaag attattaaag gcaggttata aaggatataa tattcaagaa acattagtct
      721 atgcgcgaac tggcaaagat atgtatgcaa gaagaggcgg aatgaaatat cttggttctg
      781 gtcttatggg tcgttatcgt atctggaaag acggtttagg gtcagtaaaa gattttataa
      841 ttgtttcttg cggacatatt tttattagct tactaccaaa tcaattaaga ggaaaatttt
      901 atcaaacgca attaagaaaa taggaggttt ttctttgtat agcactgatc caaaaatgaa
      961 aaaaatacaa cagattacgt tgaaaatgtc tcaagagatt gttgattttt gtctaaaaaa
     1021 tcaacttctt tgttatttct gcggtggtgg tgccatcggt actgtgagag aacaagggtt
     1081 tattccatgg gatgatgatt tagatttctt tatgcctaga gaggattatg aaaaatttta
     1141 tcaactatgg gttaattcag aaaaaagtaa aaaatatccc atacagaagg cttctaaaaa
     1201 ttataatgat cataattctt ttacaactat ccgcgatgca gatactacat ttattaaaac
     1261 ttatcaaaga gatttagata tagtccatgg aattactatt gatattttcc cgttagatgt
     1321 tgcccctgtt tcgaaattta gccgtaaaat tcaaaaacta tgggctttag tatatgcttt
     1381 attttgttct caagtcgtcc ctgaaaaaca tggtggaata atggctacag gtagtaagat
     1441 actcttatct attttccgct cacctcgtac gagatataag atatggtctt ttgctgaaaa
     1501 acaaatgaca aaatataata atgaaccaac gggctatgtg actgaacttt gtgtcggacc
     1561 taaatacatg ggaaatgttt atcctgcaaa agactttgaa tctgctgtgt gggtgccatt
     1621 cgaagataca gagatgccag taccagttgg ttatgataat tatttaagtc aagtttttgg
     1681 tgactatatg cagcttccgc ctgaaaaaga tcaggtttct catcatgaag cagtttatat
     1741 tgatccagaa catccataca caatgtacaa aggaaaatat tatttaacta aaggagcaga
     1801 aaaaaaatga ttacagctat tattattgct ggtggtgtgg gtaaacgaat gggacaagat
     1861 atcccaaaac agttcattac aattgaagga aaacctatta ttatctatac tttagaatca
     1921 tttcaacacc atcctcaagt tgaccggatt ttagtcgttt gcaaatctgg ttgggaagaa
     1981 acgatgtggg cgtatatcaa agaattcaat attagcaaag tcaaatgggt cattacaggt
     2041 ggatcaaagg gacaagagtc gatcaataat ggtgttcagt ttttgaaaga ctattctgaa
     2101 gatgatgata cgatcatcat ccatgatgga atccgtccac tagtagacga actggtattg
     2161 tcagatgtaa tcgttaaatg taaagaattt ggtaacggtg taacctctct accttacaac
     2221 gaacaaattt ttatcaagaa aacagaagaa acgacggaac agtatgtcga tcgtaacaca
     2281 ttgaggcgtg tctcaactcc acaagcttat caatacggaa aactacgggc agcatatgac
     2341 cgggcagtga aagaagacat cggtatgact gattcttctt atacgaatac catgatggta
     2401 gatttgggag aaactttata ttttgctgct ggttctgata aaaatatcaa attgacaaca
     2461 acagatgatc tagaattgtt caaagcatat ttaaaaatga aagactaggt gaaagatgat
     2521 gacgttgatt aaacaaccaa tgtaccaaga agatttggca aaagtaatca atcatacacc
     2581 aaaaataaaa cagctacatg gaaaatccgt tctgatgatt ggtgcttctg gaatgatcgg
     2641 ctcttttttg attgatacgt tgatggctgc taatgaacaa ctaggcatca aaatcaaagt
     2701 ttatgcaatg ggcagaaacc gtcagaagct tgaaaaaagg tttgccagtt atcttgaatt
     2761 agatactttc gaaattgttg aaggtgacgt cacagaaccc ttaccaaaag aaattcaagc
     2821 tgactatttg atacacggtg caagtaacac acatcctaag gcttacgcaa ctgatccaat
     2881 cggtacgatc atgactaatc ttgcaggtac agagcaagta ttgaaacatg cagtagcaac
     2941 ccaaccggtt agagtattat ttttatcaac agtagaaatc tatggcgaaa atcgaggcga
     3001 tatagaaaaa ttcacagaag attactgtgg atacatcgat tgtaatacac tgagagcagg
     3061 ctaccctgaa ggaaaaagag tcagtgaatc attatgccaa gcctacatcg caaaacacga
     3121 cctcgacatc gtgattccaa ggctttgccg tacgtttggt ccgacgatgc ttttatctga
     3181 tacgaaagca tcctcacaat ttattttaaa tgcagtcgaa caaaaagata ttgttttaaa
     3241 aagtgaagga aaccaatatt tttcgtatgt atatgttgga gatgctgttt ctgctattct
     3301 tcatttactg cttgaaggag aaaaaggaga ggcgtacaac gtttcttcag aaaactttga
     3361 tttacgacta aaagaattag cccaacagct agctgatatc agcggcaaac aagtaatttt
     3421 tgaattgccg gatgagattg aacaaaaagg tttctcaaaa gctagcacag cagtacttga
     3481 aaataaaaaa attaaagaga caggttggaa accaattttt cctttgaacg aatcattggt
     3541 tcatacagta gaattagtta aaggtgagaa atataatgtg tgagattagt attattgttc
     3601 ctgtgtataa agttgaaaaa tatttaagaa aatgtgtaga ttcgatttta gcccaaacat
     3661 ttactgattt tgaagttata ttagtagatg atggatctcc tgacaatagc gggaaaatct
     3721 gtgatgagta tgctgaaaaa gataatcgag tacgtgtgat ccataaagaa aacggcggat
     3781 taagcagtgc tagaaatgcg gggattgatg ttgcgagagg caaatactta ggctttgttg
     3841 acagtgatga ttatattgat gaagatatgt atgaaatttt gtatgagaat ttgaaaattc
     3901 atgatgccga cattagctct gttgaattga ttccttttta tggtgataga tataagaaag
     3961 ctaataaaga aaagaaagta attatattaa ataaaaagga agcaataaaa tctgttttag
     4021 aaggaacgca attttatgca tacgcatgga ataaacttta tcgtaaggaa ctttttaaag
     4081 ataatcgtta cttggatgga aaaacatttg aggatgcata tataataata gatctattat
     4141 ttcaaacaga aaaaattgtt gtttccaatg aagagaaata tttctatctt caaagaaatg
     4201 atagtattat gggaaaaagt ttttctatga ataatttcga tgttatagaa gcatggcaat
     4261 ataacaaaga gaagatttta gatgcttttc cagatttgca tgattcctat tatcgccgac
     4321 tatgttgggc atatttttac gtgttggata aaatggtttt atcaagtagt tatcaacaaa
     4381 ttccacgtaa aaaaacagta attgaatttt taaagaagaa cagaaatttt atattaactt
     4441 ataagggatt cactaaatcg cgcaaaatag ctattattgc tctgagcttt agagtagatt
     4501 ggtataggaa attggttgaa caaaaaaata aaaaaaatta gaatgtttaa attagtaaat
     4561 aaaagaaaag gaattgaaaa ctaaaatgtg cagaataagt ataatagttc cagtttataa
     4621 tgttgaaaaa tatttaaaaa agtgtgtcga ttcaattcta ggccaaacat ttaccgattt
     4681 tgaattatta ttaattgatg atggttctac agataattca ggaagtatat gcgatgaact
     4741 tgcgaaaaca gacaaccgaa taaaagtgat acataaggaa aatgggggtc taagtgatgc
     4801 cagaaatatt ggtattgagg tagcaaaggg agattttatt gggtttatag atagtgatga
     4861 ttacattgat gaagatatgt atgcattttt atataacaat atattgaaat atgatgcaga
     4921 gttatcaatg tgtggtattt atgacgtcca taagaataaa gaaattaaaa agttaactcc
     4981 tttttctcaa ttggttacta aatctgaagc aattgaatta gtattagatg gtaagctagt
     5041 tgttgctaat gcagtaagca agctatataa aaaagaatta tttgagaatg tgagatatcc
     5101 aaaaggaaaa attgctgaag acgcagcagt aatactgaaa attatcaatc agtgtaataa
     5161 aatccatgtt gatacttctc aaaaatatta ttattat
//
LOCUS       DAZKCY010000142         5156 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000142 DAZKCY010000000
VERSION     DAZKCY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.143"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..266)
                     /locus_tag="ACG8XC_002477"
     CDS             complement(45..266)
                     /locus_tag="ACG8XC_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192143.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(263..553)
                     /locus_tag="ACG8XC_002478"
     CDS             complement(263..553)
                     /locus_tag="ACG8XC_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192144.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(550..1734)
                     /gene="mobT"
                     /locus_tag="ACG8XC_002479"
     CDS             complement(550..1734)
                     /gene="mobT"
                     /locus_tag="ACG8XC_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP9192145.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(1916..3319)
                     /locus_tag="ACG8XC_002480"
     CDS             complement(1916..3319)
                     /locus_tag="ACG8XC_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9192146.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3341..4114)
                     /locus_tag="ACG8XC_002481"
     CDS             complement(3341..4114)
                     /locus_tag="ACG8XC_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192147.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4124..4501)
                     /locus_tag="ACG8XC_002482"
     CDS             complement(4124..4501)
                     /locus_tag="ACG8XC_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9192148.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4522..4836)
                     /locus_tag="ACG8XC_002483"
     CDS             complement(4522..4836)
                     /locus_tag="ACG8XC_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9192149.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
BASE COUNT         1510 a         1031 c          891 g         1724 t
ORIGIN      
        1 cataacaaaa tatatacttc ctattttatc atgaaaaata tctgttaagc accaatgatt
       61 cggttaaata agtttaataa cacatctttc acgccagctg cattgaacac aagtccgacg
      121 gcgattagag caataatcaa gaagccaatg agttttgaaa attctcgttt aaaacctaaa
      181 aacagtccaa tgacaacaat cgctaacaat accaatgact gtgcattatc caagaaccag
      241 ttgtacaaat tctgcccaaa attcatgtga ttgtcctcct ttccttaaga tatttttctc
      301 gataatagat atagcttttt acacgcctta ataattgaaa aggggttaaa tgctctccat
      361 agacggttac tcgattggta taattattat aaaatctcac cttgtaaaaa tcataggtat
      421 agatggtatc accagcttgc cgttttccct caataatgag agacgtatat ttccctgtat
      481 tttgtatata cgcttgaaac tcaatgccac actgaaaccc taacttcttt aattcttttg
      541 ttaaaaggct catgtaatca aatcctccat ggctagatgt tgttgttcaa ttaatttctc
      601 atgtttttct gtcagtttgg cttcctgaat catggtggag ataatggttg taccgttgag
      661 aacatctaaa atgcttgcga tttttaatgt aggggcgact tgatgatgta accagttcaa
      721 tgttcgttca aaagaataag gctctggctg tgtggttagg cgtaatgctc cacgattttt
      781 accgataaac cattcccaac gttcattagt tttccaatca ctccgacgtt tagtgtcgtc
      841 tttatctgca aagcggatat agcgattgat aatttcaaag gctgtttttt ccgcctgttg
      901 atgtgccaat aaatctttca tggcttgggt ggctcggtca ttttttaacc gaatttcaaa
      961 ccgattcttt acttctgcgt cttctaaggg aacatcattt ttcacgtatt gctcataatc
     1021 tttctcatat atacagaaat agacttcact ttttaatgag ccaatataaa gtgtgtttcc
     1081 catgtatggt ttttcatcac ggtgaaccaa ttcaccactg cgataatttt taaagcttcg
     1141 aaagacggaa atgcattctt cctgtttaca tttctttgcc aaatctggaa tattcaaaat
     1201 ccctgtttta tcgttgatag ctaaatccaa gcgtttgaaa atacctttat gttccatgca
     1261 atcttgaaag aaatcatacc agctccgatg ttgagccaat agaaagtttt caaattgccg
     1321 acagcctttt cctttcagtt ctaataaaac acctttactt acatcatgag agactaggac
     1381 aacaatatca ccgaaacgat agtgttcggg ataagaataa tagccaaagt cttcgtgtag
     1441 catataatcc atattcaaac gtaaaacctc accgataatt tgagccacat cattggtagg
     1501 gaatcgaatc cgtacatagt cgaataacat ctccaatggg ttatcgggat tgaagcgttc
     1561 caaagcgttc agtaaatctt ctttcacctg ttgagtaggg actgccttgc ctgtttcaat
     1621 atccgatagg taaggtctgg taatatgaga agcaaccgcc agtttatttt gagaaacgcc
     1681 ataagccagt cgcttttctt tcaattcctt aatccaaatt ttttcgtttt ccattcgcaa
     1741 acctccaatc taaaaaagat gtaaactatg acccttgagt ttacaccttt catattttcc
     1801 gcaatccttt gtaccatcaa cattggttga tgagaattgc ctgttttcag ttgtatttgt
     1861 gcccccctgt tagatagagg gggctaaagg attggcgtgg ctaacgccac accaatcaaa
     1921 gcaagtctga acctgtacct ttcacttcgt gcggtacagg ctcagattgc tttttttgaa
     1981 tgacttttcc tatttctcct aagaaatcgt acctttttgg tacaaggggt gtataaaact
     2041 cactaatcac actttttcct gtatccacgt aacctcttcc cttgatgggt tttaagaaaa
     2101 attgtttttg tgtatcgctt ccgaacatca taccaaagcc catttcagac atacgaccca
     2161 aagccacacg gaagttaaag ttgtcccgaa tcccatcact aaaatatttt gcgtctggac
     2221 gttgacaggc aagaattaaa aagaagcctg cttgtcgtcc taacattaca atttgtttga
     2281 gtttatgaat aacggtggta ctttcacgac ctagcatatc catcattgcc acatattcat
     2341 caaaaataag aaaattggca gacaaaccaa gataagcata attttctcct gttttatagt
     2401 tagggtgttg tttcatggtt tcactgcgat caatcatatc ttcgtaaaat tgattgatac
     2461 atgccaacat atcttcttta cggtaataga cattattcat gaccgcccct aagtctgcca
     2521 aatcagcatt tttagggtca agaatataaa gctttgcatt tgtatgcagg agagattcaa
     2581 tcaatgtgag aataaaatag gattttccac taccagtgcc cccagcaatt aacatgtgag
     2641 ggagagaatc gaactcccaa tagaccgatt ccatcaattt gacacgtcca tcttgagcta
     2701 ccacgtcttc aatgccaatt cgattgccaa tagtatcata aagtaggaca tactctacat
     2761 aagaatcttt caacgttttt tccactaatt cacaatacaa tcctgtttct agtttgtttt
     2821 ctaggtgcaa aagctgatct tgatatttcc ctaaggtgat ttctacatga atatggatta
     2881 gtccatcttt cattcgataa tatattttag gaaaatggcg gattcgttgt ttggttttct
     2941 tgctgggtaa atctttaaag aatccctctg attgagtttc ttcggcttca taccaatgat
     3001 tttctaaaat catacgagct aacttttgac ggtgctctaa ctgttttata gaatctatac
     3061 gatagtgata ataaagccag ctgataagtc cgcatacaaa aagagcagtg agaatactgc
     3121 tccaaaagta tggcgtatga agttgataga aaaatgtatc taactgaact gcttgccagt
     3181 tcaccgattg taacgctctc caatgaaaag ggagtaggac gactagaaaa actaccagca
     3241 atgaaattag tacaaaatga tagaccaagt gcttatcgtt ggctttgatt cgctctcctc
     3301 tgtcatgaag caatggcata aaaatcctcc tttccaccta tcaaaataat tgtgtattgt
     3361 agatacgagc ctgtttatca aaagttggtt gagaaatggc ttcatatttc tgataagcga
     3421 ttcgcttttc aaagcgtaaa tttttttctt tcggataata atgacgtcga tagagagtgt
     3481 gtcctaacag ttttttcgct tcttttgtca acggatagag atagcgaaac atgcgtccat
     3541 tgattttctc aatccctttg tattcgcaaa atgcctggga aagccaatgc tttttctcta
     3601 cctgttcaaa gcgagcattt tcctctaata aaaggcgagc acttcgaggg tgtatctttt
     3661 cccatgactg cttatcacga tagacacttg tcttgaaata cccgcagtaa aagaagttgg
     3721 acgcttgata gacatagcca cattttccct caattccatc tgccagtgta tataaaaaat
     3781 gacagtccgt atgttctttc aaccacttaa ttagagctga aagagcttga ctgccaaaat
     3841 aattggtttg attcatttct ggaagaaaac acatctttcc aatttctaaa taatctgtgg
     3901 tttgaaggga actgtcggga aagagtttgc gtatggtttg taggggttgc gtaccccagc
     3961 ctaattccac aacgcctaat aatttttctt ctgaaaaaat ccctagaaag tatttacaca
     4021 atctagggag tatctttgaa taatggtatt gccgaataaa agaaatggct tcatccttcg
     4081 gaatttcttt caaaatcaat ggtgtgtttg tcataagcaa cacctatttt tcactttcgg
     4141 acgcttcttt tttatatttc tgatgaggtt ttgtctgtcc ttttaataca agatcgtctg
     4201 ctttcacgta ccaatcaacg tctgcccctc gaaaagtagc cgtcgctacg gtatctgcca
     4261 caggattgat taattccact tctgcgttgt agtcaaattc tttcaaagga acagaagcgg
     4321 ggatactgac ttgtatcatg cgtccttgtc cttttgattt taagtcatag gtacgttctt
     4381 taatttcttc ggaaaccgta ccatcttcat tctggatacg aacctcacga cgcaaggcgg
     4441 aaaatttcaa tgtgccaaat gtctggtctt tttcaatcac aataccttct gctaatctca
     4501 tattttttca cctttccttt cttatgcttt caccatatcg tctgcgtgca taatgtaatt
     4561 ggtaaatcct cgattgccaa ttttataacc ttctgcggta atatgtgggt tgatgagttt
     4621 gactttttcg tcggattcaa aatgtttctc gccagcttcg gagggaagaa tgacaacaat
     4681 atcatctgct cgttgaatcg ttgagtataa attgtaacta cgagagagaa tggttggtct
     4741 gccatttatt cgacgttgtt caatgttccc ttcaccagca tattctaatt gtccaaatgt
     4801 tttttccata ttgggaatga catatttaag ttccatattt ttacctatcc tttctgtttt
     4861 tatgtttgaa tgaatcttat ttgtgtgatg gttttaaagg ggggagagcg accttttgat
     4921 tcttgatttt ttattttcat aagttcactt cctttcaaaa ttgggtaaaa aaatagacgc
     4981 tccgtactga gcgtccgatt cctactatat ttaaaacaac gactacctag gtattttaaa
     5041 tattataatc aaattctatt ttatctaata attgagtggg ataaagcaag tctcctttac
     5101 tcgtaaaata acccacctct gagaaaagaa ataaattatt ttttgctctt gaacaa
//
LOCUS       DAZKCY010000143         5099 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000143 DAZKCY010000000
VERSION     DAZKCY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.144"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1564
                     /locus_tag="ACG8XC_002484"
     CDS             47..1564
                     /locus_tag="ACG8XC_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFP9192150.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1536..2825
                     /locus_tag="ACG8XC_002485"
     CDS             1536..2825
                     /locus_tag="ACG8XC_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9192151.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3000..3896
                     /locus_tag="ACG8XC_002486"
     CDS             3000..3896
                     /locus_tag="ACG8XC_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9192152.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3910..4773
                     /locus_tag="ACG8XC_002487"
     CDS             3910..4773
                     /locus_tag="ACG8XC_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9192153.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4779..>5099
                     /locus_tag="ACG8XC_002488"
     CDS             4779..>5099
                     /locus_tag="ACG8XC_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFP9192154.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLI"
BASE COUNT         1597 a          795 c         1065 g         1642 t
ORIGIN      
        1 tttcaacgag gtgtattatt catgtataaa ttaaaggagg tcagttatgg aagccacaat
       61 gttgattagt agtgtcaagc gtgtaggcaa agttgatccg cggttgtaca gttcatttat
      121 tgaacatatg ggacgagcag tatatgaagg gatttatcag ccagatcatc catcagctaa
      181 taaaagtgga tttcgccaag acgtcattca cttggtaaaa gaattgaaca ttcccatgat
      241 tagatatcct ggtggaaatt ttgtgtcagg atttcgttgg gaagacagta ttgggcccat
      301 agcaaataga cctagaaggt tggatttagc ttggcggaca attgaaacga atgaagttgg
      361 gattcatgaa ttttataact ggtgtcagca tgtgggggcc gaaatcaata tggctgtcaa
      421 tctgggtact cgtggagtag atgcagctcg taatttagtc gaatattgta attttcctaa
      481 aggaacttat tggagtgatt taaggcgttc gaatggtcaa gagagccctt ttggaattaa
      541 gacatggtgt ttagggaatg aaatggacgg tccttggcag attggacata aaacagcgga
      601 agaatatgga cggttagcag aggaaacggc taaggcgatg aaactcgtag atgattcaat
      661 tgaggtggtt ttatgcggaa gttcgaatcg gcaaatgcct acatttggtg attgggagtt
      721 aacagttctt gatcaagcgt acgatcaaat agattatttg tctctgcatc aatattatgg
      781 caaccaaaaa aatgatcttt ctcattatct tgcacgttcc ttagacatgg atcggttcat
      841 ttctgaagtg acagcgatgt gcgatacagt caaggcgaag aaacatagta agaagcagat
      901 caacctgtcc tttgatgaat ggaatatttg gtatcattca gcggaacaag atgagcaagc
      961 agcgccttgg cagattgctc ctcctttact agaagataac tataattttg aagatgcatt
     1021 actattaggc tgtctattaa tcactttatt gaaacatgca gatagagtaa agattgcttg
     1081 tcttgctcaa ttggtaaacg ttattgcacc aatcaggaca gaaacaaacg gagaagcttg
     1141 gagacagacg atctattatc catttatgca agctgctgtg tatggacaag gagaggtgtt
     1201 aagcccacaa attcgttcag caaactatgc gacggaagat tttgaagaag tgccgtattt
     1261 agaaagtata gccgtaataa atgacaaaga acttgtcata tttgcagtta accgtggaga
     1321 agaagaaatg aatctaactg ttcacttgtc tgaattatca ttagagggag tgattgattt
     1381 ttctgaaatg agtggatttg atacaaaagc aactaatctt cctaaaagtg aacaagtaaa
     1441 accaagtcat tcaacaaatg taaaagctga agatgggaaa atcaattgtt tattgaaacc
     1501 gctatcttgg aatgtggtca gatgtcgttt atcagatgaa ggaggaacaa aaaatgaaag
     1561 gtaaaaaatg gttaattggc tctttgttgg tagctgcaac aggtatttta ggagcttgcg
     1621 gtggcggaag ttcagcagaa tctgattctg ataaagaagt gactttgact ttttggaatg
     1681 ggtttactgc atctgatggt gaaatattgc aagaaatcgt taatgaattt aataaaacaa
     1741 atgataaaaa catcaaaatt gaaatggata tcatgacttg ggccaatttg aatgaaaaac
     1801 ttcctacagc gatttcttct aaaaaagcac ctgactttat tgcattaaat tatccagatt
     1861 ttgcacagta tgtagaaaac ggagcggtac aaccattaga cgatttctgg gaatatgatg
     1921 gagtagataa atctgatttt aaagaaacgg ctattgaatt aggtgaagta gaaggtaaac
     1981 agtattttgt ccccatgcag gtacaaggca tgtatatgta ttggaataat gatttgttta
     2041 aacaagctgg attagatact gaacaaccac ctactacttg ggaagaattg acagaaatgg
     2101 caccaaaatt aactgatagt agtaaaaatg tatcaggttt tgtcttcaat aaggatggaa
     2161 ctgcaccgtt atataactgg atgttagcaa atggcggtaa attagtgaac gatgattata
     2221 ctaagtctga atttgcgtct cctgagaacc ttaaaacgtt aaaagcgatc caaaaaatga
     2281 tccatgtaga caagactggt ccagaatcga tttctggcgc cgaaatggac aatttaatga
     2341 atgctggtca actagccatt gaaatcaatg ggccatggtt aaataacgga ttgaaagcaa
     2401 acgagattga ttataatgtg actaccttac cacaagggag tgattcagac aaaaaaacag
     2461 ctattttaga tggcgtagga tatgccattc cggcaagtac agataataat aaaaaagaag
     2521 ctgtctatga gtttttgaaa tattggaata caactgaaat tggtaaaaaa tggagtatcg
     2581 aaaatggttt tcctgcctac ttggattcag tagcaaaaga tgaagaagta aaaaatgatc
     2641 ctattgtatc tgaattatcg aaacaaatgg actatgcaga gccatattta ccaggtttta
     2701 cccaaatctc tgcgatcaat aacgatatca tcaatccact gattgaaaaa ttattagcag
     2761 gagaagatcc tgagacactc atgaacgatg cagatgatgc catcaatcaa ttactgcaaa
     2821 aatagtacag aactttatac aggatagact gttggcaaga tacagatatc tgaatgatcg
     2881 acctagtatt tgcagatagg tgttattgca aaaatcacag cgattgttcg gatatcttat
     2941 tcagattcgt ttaaaccaag attgtatatt ttatcaaaat gaaagatgag gtggactata
     3001 tgaacggatt caccaaatat tggaatcgtc ctgatcgtgc tgcgtatttg tttttaactc
     3061 catctttttt agttttatta ttatttactg tcataccgct tttaggaaca tttgctatta
     3121 gttttacaga tttgaatatt ttctttacat caagaaattt tgtaagtttt gaaaatttca
     3181 cacgtatatt tagtgatgaa cgagcagtta attcgttgat tcattcttta tatttcaccg
     3241 tactcgaaac gccaacacaa attatcgttg gtttattagc agcagtagca ttatctaaaa
     3301 ataccagatt caatcgtttt tgtcgttcga cattttacat tccagttatt tgttcattga
     3361 cgtcaattgc gattattttt tctatgttgc ttgatccgaa tgtaggggcg attccttact
     3421 tgttttcaca agtcggcttg ccgatccctc aattttttcg cgatccaacc cttgctatgc
     3481 cgacggtagc catcatgact gtttggaaga attttggtgt tactatgacg attctcttga
     3541 ctgctattca gggaatctct ccctctttgt atgaatctgc tcaaatggat ggtgcaacaa
     3601 atcgacagca gttttttaac attacgctac ctcagatcgt tccctcctta ggtttttgta
     3661 ttttaacgaa tctgattggt tcgatgcaag tgtttgacca agtatatgtt gcaaccgctg
     3721 gtggcccaca atttaagaca gaaacagctg ttcaatatat ttacagtcga ggatttacag
     3781 caccttatga attgggctat gcatcagcac tgtcttcggt tttgtttatc attgttgcaa
     3841 ctatagctat ctccactaat gtgtacatgt cacggaaaga aaaacaaatg aaataggagg
     3901 gaaacctgta tggcaccaga aagctcgatt acagttactg tgaatagtcg aaaagcagtt
     3961 agtggtaaaa aaatcattcg aacgattagt atgctagtta tattgttggt tattgcgttt
     4021 ccttttttgt ggctgattat ttcttctttt aaacacgaaa aagacattat ctcatttccg
     4081 cctagaatat ttgcagatag ctatacccta gataactata ttaaggtttg gacaacgatc
     4141 cctttattag attacatcaa aaatacagtg atttttgctg gagggacagt gatcacttct
     4201 gtattctttg attctcttgc ggggtatgca tttgctcgta tgcgtttcaa aggaaaatca
     4261 gttttgttct atttcgtttt gctgacaatg atgatcccat tccaagtctt catgattcct
     4321 ctatttattg aagtcaattt gcttggcatg cttgatactt atgcaggttt gatcattcct
     4381 cgtatgacaa ctgcatttgg tatcttcatg atgcgttcgt tttttattac tttaccagat
     4441 tctttggaag aggcagcacg cattgatgga ttaagtgagt ttaatatttt tttgaaaatc
     4501 atgttgccat tgagcaaacc tactctttta tcattaggga ttttcacgct gatgaatagt
     4561 tggaatgact tgctttatcc attgatattg acaagtagtt caaaaatgag aactttacca
     4621 gcagggttag ctttatttac agggcagaac atttcttttt atggacctgt aatggctgga
     4681 acggttattt caatgctgcc attactagtg gtttatattt ttgcacagaa atattttgtt
     4741 caaggaacag ctatgtctgg aatgaaagag tgaggaagat gaaaatagtt gagactaaat
     4801 tttatggaca gttgattgtg gtttctgatt acatcttatt aggtattctc tgggtaatcg
     4861 tgtgtttgcc tgttattact atcgtgcctg ctacatgtgc tgttttttat gtaatggcgc
     4921 aatggaaagg tgagggatca ggaaatatca tttatttttt ctttcaagga ataaagcaac
     4981 attttggtaa gaacttgttc cttagtctgt taacgatcat tatttatttt cttgttaatc
     5041 aattattaga agaacagaca atcgtacttt caattagtgg atatgtagtt ttacttatt
//
LOCUS       DAZKCY010000144         5088 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000144 DAZKCY010000000
VERSION     DAZKCY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.145"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..660)
                     /locus_tag="ACG8XC_002489"
     CDS             complement(205..660)
                     /locus_tag="ACG8XC_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LMxysn_1693 family intestinal colonization
                     protein"
                     /protein_id="HFP9192155.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1269..1685)
                     /locus_tag="ACG8XC_002490"
     CDS             complement(1269..1685)
                     /locus_tag="ACG8XC_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192156.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTITTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            complement(1687..1968)
                     /locus_tag="ACG8XC_002491"
     CDS             complement(1687..1968)
                     /locus_tag="ACG8XC_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192157.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            complement(2415..3383)
                     /locus_tag="ACG8XC_002492"
     CDS             complement(2415..3383)
                     /locus_tag="ACG8XC_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9192158.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            complement(4550..4705)
                     /locus_tag="ACG8XC_002493"
     CDS             complement(4550..4705)
                     /locus_tag="ACG8XC_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192159.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4724..>5088)
                     /locus_tag="ACG8XC_002494"
     CDS             complement(4724..>5088)
                     /locus_tag="ACG8XC_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFP9192160.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1595 a          839 c          839 g         1815 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacactatg ccaagtagcc cctctaccaa tggatagaga cgctcacttt
      841 gtgatttcta attggtctag cttaaacact tcagccaatg cttgatttta tgcacttttc
      901 ttttttcatt tctttgtgca ttttgaccgg tcatttttta gagatttgtt atttttgtga
      961 gcatttgata tgctcttttt atacctcaat ctcttctttt ctaatgttaa tgaataggat
     1021 aatctcattt ttagctgctc gtgagagcag tctttttttg tttcagggta cgtcctattc
     1081 tttggatttc cgcgctatta aataaccgat acttggggat tggggtagcg ccccaaaata
     1141 caaaaagaag aagttgcata agcggatttt tctttttttt cgcaagttgg taccaacttg
     1201 caaatcttgc tggaagactg atccctaaaa agaaatcgag gaattttttt agggatcggt
     1261 ttcttttttt atctttttag aaagtttgtt gggtcaaaat ttccagaagt aaaggtttga
     1321 ttggtttctt tttgacgatt tttgggttga tttcgtcggg aaggattgag ttgattttca
     1381 attttttctt tcacttcttg atacacttta gaatttgtac cgataagaac accagtatct
     1441 tttagatata aatcagcaaa ttctgccatg atttctgtca tgatagaaat ttcttttccc
     1501 atggcattga gttttgcttg gttactggtt gtgattgtat tggtatcagg ttcataactc
     1561 atacataaaa aacgaatggc ttctgaataa ttgttgacgt ctggattttt ttcgatgatg
     1621 gaatctaaaa cgtcaacgtg actgggataa agggttacgt gtttttgaac agaagttttt
     1681 ctcatgttat attcctccga taagtgtttc aatgagttgg ttgtttgtgt gaacatagtc
     1741 attcagtatg ttcgtatttt ctgtttgaat ggcaataaat ttttcgagta ataaatcgaa
     1801 ttgatctttt ttgtaattca taagttgacg gtcaaaatca ctttcgatta gaacttttag
     1861 atatttattt cgactccatt ttttgcccgt tttggttgtg agttcctgac aacgtttatc
     1921 tattcttgca ataattttgg gattgatatt tcgaactaaa atgtccatgg aaattttcct
     1981 ttcgtatcag cgtataattt atgtatatca tacaaatata gtatttgtat agctattttt
     2041 cgccaaaaca ctatacatag cctagccaca atggcaaaaa agtgatagca aatttttttc
     2101 tgagaaacgt gataggcaca gcctatctag caccttttgt aagaaagtga taccacgttt
     2161 ccttatgctc tttcaaaatt cagaaaaatc agctgtaaaa cgagtgcagt ccgagtaaga
     2221 ttcaacgaaa aaacggggat tggggcgcca ccaaaatatc gcaacatggg tccatgttgc
     2281 tctgctcgca aaagcaaaaa gaagaataaa accagcaatt taaattaata aaaattgctg
     2341 gttttattgc cagaattgcc accatttttt ctccgtcttt gattgttttt tctgtcataa
     2401 aatctaaaaa cccctcaatc tttgaataag ttttgaagat tgtctaataa gtcttttttt
     2461 agttctgaag gaatatcgga atttatctct gatttttgtg cattattttc tttattttgg
     2521 gtattttgtt ctctttctaa cttttctaac tgttgtaaaa gttccaattt tcgcttttgt
     2581 tctttgattt tttcttgtaa tgaagtaggt gctttttcag attgaacttg ttggttagct
     2641 agttctaatg ctttgttttg ttcttctttt gttggggcat gtttgttaaa gtcgtctgat
     2701 ttttttatct caggtttaaa tgtaaattga tagccaatga caggctttcc ccgtcctttt
     2761 ccgtatttct ttttgattgt taaaccttta aagattgcag ttagttcttg tcgtattgga
     2821 ttgaacacac gttgatcaat cgtaccttgg ttgtaacttt taggaatatc aagtaattca
     2881 tgaaagtctt cttttgaaaa ataagcgaaa cctttggttc tgtattgttt tagtaatcga
     2941 aacattgttt ttgaataact gctttctaat tcattgaatt gttggagaga atagcgcacc
     3001 cattcttcaa gattattaag taagggtaag gctttttcat gaacttgtat ttcagcaaaa
     3061 ggaatatcag attttccgtc gattttaaat tgattaaata aaacaaaacg ttcacgttgc
     3121 aaaccgtcag cacttcttct accaaaacgt aaatccatta atttgtcata cgttgtttct
     3181 aaatcatcta tgaatcgaac atttgcagtt gctttataat tgcttaaatc ttttaattgc
     3241 tcgaatgtaa attgtacttt tttatcacct acatcacgca ttcttgatac gatagaaaag
     3301 aacaaattca tttcgactgg tgtaaatttt cgcaacggaa ttgtatttaa ttcatgatga
     3361 tactttacga tttcattaga catatttaaa atagctcctt tcttactcgc acaagagata
     3421 cacgttatga aatacaaaat gcctatttat ctacatctta tattaacatc gtcgatgtac
     3481 taattcaatg tacaaacaga caaaacgatg tagataaaca gacaaaacga tgtagataaa
     3541 cagacaaaac gatgtagata aacagacaaa acgatgtaga taaaagcgtc attcccttgg
     3601 gagagtaagg atatagccga cggtaaagag gtttaaaaag aggtttaaaa agagattata
     3661 aaagaggggg acaaaatttc ccgcctaaaa aaccacttct tttttctcca aacagattcg
     3721 cgcatttttc tcaactggga atcttactcc ctacagtcgc ttaaaaaggt gtgatcgctc
     3781 tgctctcaca ttatactgcg cagtcgcttc cggctccttt gctaaaaata ttcataagga
     3841 actctttgag aagctatgag ccttcgtttg agacgtttta agacagtctt agtataaatc
     3901 ccttatcttg ttcttaaagc gccttacagg cggtcagggc aagccctaac aaccctaaag
     3961 ttgtctgaat gccctctaaa attcgattta agcactctta aacctattct tatatattta
     4021 tatacttaga cgtttcaaac gtcttaaaac gcaaattaga gcttgataaa gatatttgct
     4081 cgtgtgccga cttttttaaa agtctaggca cacgccatga caaagctgaa ataaactgct
     4141 tcagctttgt catggcggat tcgctcactt gaaaaagtgt tcatttcgct cgattattag
     4201 catatatttt ttaacttttt ttgctagcca aattttgcat tagttcgctc ataaatccca
     4261 ccctttcagt acgtcctacg gacgtatcgt tcgtaatgtg aaagtacctt taaaggtact
     4321 ttctacaaaa ctcactaaag ggtttggcaa gctctatatt tgctagccaa atacctacgt
     4381 atttggtgca aataaccgct tgtcaaaggg gcgcaagctc ccccttgaat cccccctcaa
     4441 aacccacgct ccacaataca aaaaaggcta gcaaatgcta gccacttttt gtaaagtttc
     4501 gcttgtttca ccttcggtga aattgttgct ttgcaacgat caggtagagc tacatttttt
     4561 ttcgttgttc aaatattctt acgccattta ctattaaaat cgtaggtatt ccaaagtaaa
     4621 gaaaaaattg attagcaggt aatacaaaat ataaaataat accagcaata attaccaaac
     4681 ttatagtaag ttttgttaat ttcatgaagt atgccacctt atcttatata gatatcaaat
     4741 agattaatat gccgattgta ttataaaatc catgagcaac tatggaatac catatatttt
     4801 ttgtttgtat ataaacaaaa cttaaaataa taccaataaa gaaaatactt aggcctcccc
     4861 catgaacaat agaaaacaga aaagaagaaa ttagtattga aatatattgt tgttttttgt
     4921 ttgcttgagg atcaatcata caaaatcgaa aaattccttc ttctaaaaaa gctccataag
     4981 tagcagcatt taaaaagaat aaaaggagag aaccatttac ttcattagga tttatacctt
     5041 ctgaaaaaga tgtatttaga atattgatta ggaataaaac agagaatg
//
LOCUS       DAZKCY010000145         5075 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000145 DAZKCY010000000
VERSION     DAZKCY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.146"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..1873
                     /locus_tag="ACG8XC_002495"
     CDS             158..1873
                     /locus_tag="ACG8XC_002495"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9192161.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1876..3639
                     /locus_tag="ACG8XC_002496"
     CDS             1876..3639
                     /locus_tag="ACG8XC_002496"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9192162.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3679..4215)
                     /locus_tag="ACG8XC_002497"
     CDS             complement(3679..4215)
                     /locus_tag="ACG8XC_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP9192163.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     regulatory      complement(4253..4351)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(4538..4960)
                     /gene="mgsA"
                     /locus_tag="ACG8XC_002498"
     CDS             complement(4538..4960)
                     /gene="mgsA"
                     /locus_tag="ACG8XC_002498"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP9192164.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
BASE COUNT         1527 a         1018 c          979 g         1551 t
ORIGIN      
        1 tttaaaaaaa atagtctatt caatttctaa agcttcttca taataaaact ttcatttatg
       61 aaaaggcttg ttctcttttc attctaaatt tcctaagcta caatgtggta gtacttgttt
      121 tttcaattgt ggacaaataa ataataggag gaatcatatg attcagttat tacgttatgc
      181 aaaagactat cgtaagcaaa tcattttggg cccgttcttc aaatttcttg aagctgtctt
      241 cgaacttgtc ctgcctttaa tgatggcttc cttgatcgat aatggactaa aaatgaatga
      301 ccgaggaaaa atcattgaaa tgggcttatg gatggtcgct atgtctgtga ttggtctgat
      361 ttgtgctatc atttgccaat actatgcttc agtagcttca caaggatttg gtacagagtt
      421 acgtaatcag ctaatcaaaa aaatcaatac tttctctcac aacgagttga atcattttgg
      481 aacagatacc ttgatcaccc gaatgacaaa cgacatcaat cagcttcagc tagctttagc
      541 aatggtcatt cgtttattga tcagagcacc gtttttaagt atcgggtcag tggtcatggc
      601 ttttgtgatc gactgggaag taggtctgtt tttcttagca ttattgccta ttttctcaat
      661 cattttattt tttatcatca aaaaaaccgt tcctctctac caaaaagtgc aagaaaaact
      721 agatcagctc aatgaacacg ttagtcagaa tcttagcggt gttcgtgtca tccgcgcatt
      781 tgcgcagaca aaaaaagaaa cagagaagtt cgataaaagc acaaatgatc tagcgaacaa
      841 ttatttacgt gtttccaata tctctgccct attatcacct gctacaacct tgatcatgaa
      901 tgtcgggatc atctgtttgc tgacagttgg cgggatcaaa gtaaatattg gtagcttgca
      961 acaaggacaa gttcttgctt tgatcaatta tatgaatcaa atgcttttgg cactgatcgt
     1021 tgtttcaaac ctcgtcgtga tcttcacacg tgccgaagct tcaggaaatc gggtgaaaga
     1081 ggtgctagac acagaaaatt cgattcttga agctgaggtc agtactactc ccgatttttc
     1141 ttctgaagca attatcgctt ttgaccatgt tgacttccgt tatactcctg attctggtct
     1201 ttctctccaa aatattacat ttcagctgaa gcgaaataca gtccttggaa tcattggtcc
     1261 aaccggaagc gggaaaacga ctttgacaca attgattccg cgtttctatg atgtcagttc
     1321 cggtacagtt ataatggacg gaatggatgt tcgttcttgg cagctggatc atctccgtca
     1381 gcaaattgct cttgttcccc aaacttctgt actatttacc gggactattc gtgaaaacct
     1441 tcaatggggt aagaaagacg ccacagaaaa agaatgttgg gaagctctga aaatcgctca
     1501 ggcagaagaa tttgtccgtc agcttccaaa tggtttagat acaaaagtca tggaaaatgg
     1561 gaaaaatttc tctggcggac aaaaacagcg actgacgatt gcaagagctt tgattgccaa
     1621 gccagcatta ctgattttag atgactcttt aagtgcgttg gattatcaaa cagacttgga
     1681 tttgcgtaag gcgctacaga cttatcttgg cagtacagtg attattatct cccaacgtgt
     1741 ccgctctatt caagaagcac atcatatctt agtgatggat caaggtaaga ttgctgctca
     1801 gggtacccat gaagaattac ttgctcaatc agcagaatat caggaaatcg tcgcatcaca
     1861 ggaggaagaa tagttatgaa agtaaaaaaa ttatccttgc agacgctccg ccgattcatt
     1921 ccttacctta ctgcctatgc aaaagacatt tggctagctg tgatactagg aatcatcaat
     1981 gggattgcaa cagttgtgat gacgttacaa attggtcaaa gtatcgatca aatgattgga
     2041 gaaggacagg tccgcttcaa tcgactgttc catttactta ttctattcgg tggtatcgtt
     2101 ttaatcaacg tagtcagtca atggctcatc caagtgttaa gcaatcggtt agcttactta
     2161 tccgttgcaa aattaagaaa agatgcattt gctcatctga atcgattgcc acttcgttat
     2221 tatgatcaaa actcccacgg aaatatcgtc agtcgattta caaatgatat ggacaatatt
     2281 tccattgcaa tttctgcggc cttcaatcaa ttattttctg gagtggcaat catcattctt
     2341 tcgtttattt ttatgatgcg tctaagtcca cttctgacat tggtcgttct ttgttctaca
     2401 ccaattattt tccttgtgag ttggatcgtt gctcgtgctt cacaaacaaa tttcgataga
     2461 cagcaaagga ttattgggga tatctcgggc tttgtgaccg aacgaatcgg taaccagaaa
     2521 atcgtgaaag cttttcagca agaagaagct agtcaacagc aatttgaact gctaaatgct
     2581 gatctatatg aaaaaggaca acgggcacaa ttttcttctt ccttgacaaa cccgtcttct
     2641 cgttttgtag atcaccttgc ttatttatcg attggtttag ttggcggcct attggcattg
     2701 aagagtggtt cgtcaatcac tgttggtgtg atttccagct ttacgatcta ctcaagtcag
     2761 ttttctaaac catttatcga actttctgga atcacgacac aactgcaaac cgctttttct
     2821 ggtctagaac gagcatttga tttgatcgat cagccagaag aaaagcctga tttgccagat
     2881 gcctatgcct tggaacccaa aaatatcaaa ggaaaagtct cttttgatca tgtttctttt
     2941 tcttatgacc ccaagcagcg tttgatcgaa gatttcaatt ttactgctta tccaggtcaa
     3001 accattgcta tagtaggaaa aacaggggcc ggaaaatcca cacttgttaa tttgctgatg
     3061 cgtttttatg acacaacaaa aggaacaatt acagtcgatg accatgacat ccgctctatc
     3121 caacgtgata cattacgtaa aagttttgga atggtgttac aagatacttg gctatttgac
     3181 agtacattgc gagaaaatct tcgttttggg aatccggaag caagtgacgc agaaatcaat
     3241 gatgccctaa aagcctctta tatgtatgaa tttgtagaac gtttgccgga aaaattagat
     3301 actaagattg gcggtcaagg gttaaagata tcagatggac aacgccaact gttgacgatt
     3361 gctcgaacga tgatcagtaa tccccctatg ttgatcttag atgaagctac cagctcagtc
     3421 gatacattga cagagaaaaa aatccaacaa gccttcttag caatgatgga aggaaaaacc
     3481 agttttgtga ttgcccatcg cctgtccaca atcaaaaatg cggaccagat attagtgatg
     3541 gatcagggag ctattgtcga aatgggcacc catgatgagc tgcttcaaaa agatggttat
     3601 taccgacggc tctatcaagc acaattcaca gcacaataaa aacaagcagt cttctgggat
     3661 gggaagactg cttgtttttt atttagcgtg gatcgctaaa cttcttaaaa tttttttgta
     3721 aggaatacta cgtcctacgt aataaatggc aattgattga atcggtaacc atattactgt
     3781 ttttaacagg cggggcgccc aaacgaccca gctgaacaat ggcacatggt acatcaacgc
     3841 aagccataat ggtgtcaaaa ataagttgat gatcagcgtg acactaagcg tagccagaat
     3901 ggcatttttc caagtgatct cttttcgata gaaaaagaat ccgtagattg ctccttcaat
     3961 aaatgcattc aatgtaaatc caataaagaa tgcagatttc gagaagaggg ccattcctac
     4021 taaatcagct aaaactgcgc ctatccctga ccaaaaagga ccaaaaagaa tgcccatgat
     4081 cacttgtgga ataaatgtga cactgatttt aataaatggg gtctcgattg ctaccagtcg
     4141 ggacaaagtg accatcaatg caatcaataa gcccataata gcaatcatac gtgcgtctaa
     4201 tcggtgtttc ttcattttta gcatctcctt taagagctgc tacatcgtat catgcagcga
     4261 atgcgaaaat aaaaccgcga acattacgtc cttcgtccaa gaggcaacat cccatcctct
     4321 tagcacttaa cgctttattt tctactctgt attatttttg ggatcttttc ccacagaaac
     4381 tatagcatat tttcacattt actgcaaata cgaaaaacta atattattgt ttatattatc
     4441 caatacgttc gtattatatc gcatattatc agtttgttta gcttttatag ctacatatag
     4501 gcgaacatta aacgttattt ttccatacgc ttatccatca aaatgaaagt ggatgcttgt
     4561 ctatttcatg gacgattctt cgaaaatcta ggaagccggc ttccagtcct cttagtagca
     4621 cctcagctgt tccgatattc gtagctaacg gaatctcata gacatcagat aaacggataa
     4681 gtgctgttac atctggttca tggggctgtg cagccagcgg gtctcgaaga aaaatcacca
     4741 aatctaactg atcctccgaa atcatggctc caatttgctg atccccgcct aacggtccgg
     4801 atttaaaccg atggatcggc aagccggttg cttccgtgat cctctgccca gtcgtcccgg
     4861 tagcaaaaag ttcatgtttt tccaatattg ttttatacgc tgttgctaat ttcaccatca
     4921 taggtttttt tcggtcatga gcgatcaaag cgattttcat aagagacact ccttttgata
     4981 ttgttaacat acttatttac tccagtgtat cagtttttat ttcttatgga tatttattaa
     5041 agcttaaaac tagcacattg acaaaaaaaa gaaaa
//
LOCUS       DAZKCY010000146         5044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000146 DAZKCY010000000
VERSION     DAZKCY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.147"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..438)
                     /locus_tag="ACG8XC_002499"
     CDS             complement(<1..438)
                     /locus_tag="ACG8XC_002499"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9192165.1"
                     /translation="MEEFGIDSISRTEALDFQADYEYELDQITMIPEIHQLLLALHKN
                     RIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGAAENSYEGNVKNLQSY
                     QNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRHK"
     gene            complement(661..1428)
                     /locus_tag="ACG8XC_002500"
     CDS             complement(661..1428)
                     /locus_tag="ACG8XC_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9192166.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1916..2341
                     /gene="lacA"
                     /locus_tag="ACG8XC_002501"
     CDS             1916..2341
                     /gene="lacA"
                     /locus_tag="ACG8XC_002501"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFP9192167.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2358..2873
                     /gene="lacB"
                     /locus_tag="ACG8XC_002502"
     CDS             2358..2873
                     /gene="lacB"
                     /locus_tag="ACG8XC_002502"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFP9192168.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2884..3816
                     /gene="lacC"
                     /locus_tag="ACG8XC_002503"
     CDS             2884..3816
                     /gene="lacC"
                     /locus_tag="ACG8XC_002503"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFP9192169.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTVSHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3819..4799
                     /gene="lacD"
                     /locus_tag="ACG8XC_002504"
     CDS             3819..4799
                     /gene="lacD"
                     /locus_tag="ACG8XC_002504"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFP9192170.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLQYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4827..>5044
                     /locus_tag="ACG8XC_002505"
     CDS             4827..>5044
                     /locus_tag="ACG8XC_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9192171.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPY"
BASE COUNT         1659 a          799 c         1016 g         1570 t
ORIGIN      
        1 tttatgacga cttttggaag taaaaatcca tccattatct ttataatcag gattgaatct
       61 tatatgttgg tgatttaact caatttcctc tttaagaatt tctatagccc ttttagataa
      121 ttggacattt tggtaacttt gtaagttttt tacattacct tcatagctat tttctgcagc
      181 tcctccgtga gtggactgac taatgatgat attttgcttt tcaaaatatt gataggcacc
      241 aatattttgg agttttcgac tctgtaagtc aacagggcca ttagttagaa tacctattgg
      301 gatgcggttt ttatgtagag ccagtagtag ttgatgtatc tcaggaatca tcgtaatctg
      361 atcgagttca tattcatagt cagcttggaa gtctagagct tctgtcctag aaatactatc
      421 gatgccaaat tcttccatag tcttctgaca gcgaaaaatt ctaagctgct caatactgat
      481 gagtttttcg tgtatttagg aaaagcaaca tcagaccaac atctgaaacg tttgtaaatg
      541 ttatctattt gttggatatc aatcgttgga aaacatttct caacgctact tttaaagggg
      601 agttgatggt cataaagagt gtcatcaagg tcaaaaacaa tatttttata agtcataatt
      661 ctagtccgct tttaaaatag tagtgtactg gctaaattcc tctaagtgtt gagggctaat
      721 ctggttatct gtaatgatgg tatcgagttg atctagatta tagaagttaa agaaatcgta
      781 tcgatcgaat ttagtactgt ctactaataa gaatttttct acagcattgt ttagggcaag
      841 ttgttgaatc acaccttcct tgtcactata tgtagcaata gaattatggg taacagcatt
      901 tgcactaaca aaagctttgg caaatctaat tgcttttaaa tttgtcgaag ccattgaacc
      961 tacaaaagct ccagttattt ctctatattc accgccaaga agcaataaat caatggtttc
     1021 gctatcattt agaatcaaga acacagggag actatttgta atgacacgaa ttttataacc
     1081 tttacgacct ttcaattcta atgccagttg ttcaagtgta gttccgggtc caataaaaat
     1141 agtatcgcca tctttgatta aagagcaggc ttttttagca atgtcaattt tttcttttgt
     1201 atttagactt ttcttctcga tatgtgtctt ttcaagtggc tttttagagg aaagtaattg
     1261 tgcaccacca tgagtacgtg ttaaaagtcc atcagcttct agagcatcaa ggtctctacg
     1321 ggctgtcata tcggaaatat ctagttcatc tattatttct tttatggtta tggtcccatc
     1381 aatctttaac atatctaaaa ttttttctaa tcgtcgtttt ttgttcatat gaagaccctt
     1441 ctttcataaa gtaatttttt tccaaagata attctctttt aattgtatca taaaagacaa
     1501 tattttcaag gtaaaacaaa caatttcaaa caaaaacaaa tgttagatga tgaaataaga
     1561 acagtggatt gacgtatatt agcttaggtc agattttgta taagacgaaa ataaagtagg
     1621 acctcttaat cagtaagtta tagaaagtaa aagacttttg taatacttga atagatattt
     1681 cacgtccatt ttgtgatgga ttaaatgaac aaaaatgaac aataatttaa cggtgttatc
     1741 tattttttaa aaaaacaaat aaaaaaacaa aaaataaaca aaaaagtgtt gctttttgtt
     1801 tgaatgcttg atattatata aacaaagaaa tgatgaaaac gttttttcga acattttgca
     1861 aaatgttttc tacttctacg tagcatttct ttctaaaatt taggaggtgg tcaaaatggc
     1921 tattgttgtt ggtgcagatc tcaaaggtac gagattaaaa gatgttgtga aaaattttct
     1981 tgtagaagaa ggttttgagg taattgatgt gactaaggac ggacaagatt ttgttgatgt
     2041 taccttggca gttgcctctg aagtaaataa agatgagcaa aacttaggta ttgtaattga
     2101 tgcttatgga gctggtccat tcatggtagc aactaaaatt aaaggtatgg ttgcagcaga
     2161 agtttcagat gaacgctcag cttatatgac acgtggacat aacaattcac gaatgattac
     2221 ggttggcgct gaaatcgtcg gtgatgagct tgctaaaaac atcgctaaag gtttcgtcaa
     2281 tggtaaatat gatggcggac gtcatcaaat ccgagtagat atgcttaaca aaatgtgtta
     2341 aggaaaggag ttaaggaatg agaatcgcaa ttggatgtga ccacattgtc acagatgaaa
     2401 aaatggcagt atcagaattc ttgaaatcaa aaggacatga ggtccttgat ttcgggacat
     2461 atgaccacgc acgtactcac taccctatct atggtaaaaa agtcggtgaa gcagtagtaa
     2521 gtggtcaagc agacttggga gtatgtatct gtggtacagg tgtcggcatt aacaatgcag
     2581 tcaataaagt tccaggtgtt cgctcagcgt tggttcgtga tatgacatca gctatatatg
     2641 caaaagaaga gttaaatgcg aatgttatcg gtttcggtgg aaagattact ggtggtcttc
     2701 ttatgaatga catcattgaa gctttcattg aagctgagta caaaccaaca gaagaaaata
     2761 aaaaattgat tgcaaaaatc gaacacgttg aaacacataa tgcacatcaa gcagatgaag
     2821 atttctttac agaattcctt gaaagatggg accgtggtga ctaccacgat taaggtataa
     2881 caaatgattc tgacagtcac actaaatcct tcagtagaca tctcttatcc tctagaaaca
     2941 ttgaaaattg atacagtgaa tagggtgaaa gatgttagta agacagctgg tggaaaaggc
     3001 ttaaatgtta ctagagtctt gtatgaatct ggtgataaag ttactgctac aggtttctta
     3061 ggtggtaaaa ttggtgaatt tattgaaagc gaattagaac aatctcctgt tagtccagct
     3121 ttttataaaa tctcaggaaa cactagaaac tgtattgcta tcttacacga gggtaaccaa
     3181 acagaaattt tggaacaagg accaacagtt tctcatgaag aagcagaagg ttttcttgac
     3241 cactatagca atcttattaa acaatcagaa gttgtgacaa tttcaggaag tttaccttca
     3301 ggacttccca acgattatta tgaaaaactt atccaacttg cttcggatga aggagtagca
     3361 gtcgttttgg attgttcagg tgcacctctt gaaacagttc taaaatcgtc ggctaaacct
     3421 actgccatca aacctaataa tgaggaactt tcacagttgc ttggaaaaga agtaacaaaa
     3481 gatattgaag aactcaaaga cgttcttaaa gagtcacttt ttagtggtat cgaatggata
     3541 gtggtctcat taggtcgaaa cggtgccttt gcaaaacatg gtgatgtttt ctataaggta
     3601 gatattcctg atatcccagt tgttaatcca gtcggatcag gggactcaac ggtagctggt
     3661 attgcatcag ctttaaatag taaaaaaagt gacgctgact tattaaaaca tgcgatgaca
     3721 ttgggtatgt taaatgctca agaaacaatg acagggcatg tgaatatgac taattacgaa
     3781 acactaaact cacaaattgg agtaaaagag gtataaaaat ggtacttaca gaacagaaac
     3841 gcaaatcttt ggaaaaaatt tcagataaaa acggtgttat ctcagctttg gcatttgacc
     3901 aacgtggtgc tttgaaacgt ttgatggcac agtatcaaga tacagaacca actgtagctc
     3961 aaatggaaga actaaaggtt ttggttgctg atgaacttac aaaatacgct tcttcaatgt
     4021 tgcttgaccc tgaatatgga cttccagcaa caaaagcatt ggataaagaa gcaggtcttc
     4081 ttcttgccta tgaaaaaaca ggttatgata catcaagtac aaaacgtttg cctgactgct
     4141 tggatgtttg gtctgcaaaa cgtatcaaag agcaaggcgc tgatgccgtt aagttcttgc
     4201 tttactatga tgtagacagc tcagatgaac ttaaccaaca aaaacaagct tatattgaac
     4261 gtgttggttc tgaatgtgtc gctgaagata ttcctttctt cttggaaatc cttgcttatg
     4321 atgaagaaat ctcagatgca ggttctgttg aatatgcaaa agtgaaacct cgcaaagtca
     4381 ttgaagcgat gaaagttttc tctgatccac gctttaacat tgatgttctt aaagtagaag
     4441 taccagttaa cgttaaatat gtcgaaggtt ttgctgatgg tgaagtggtt tacagtaaag
     4501 ctgaagctgc tgacttcttt aaagcacaag aagaagcgac taaccttcaa tacatctacc
     4561 taagtgcagg tgtatctgct aaattgttcc aagaaacatt gcaatttgct cacgactcag
     4621 gtgccaagtt taatggtgtg ctttgtggac gtgctacttg ggcaggatct gtagaacctt
     4681 acatcaaaga aggtgaaaaa gcagcacgtg aatggttgcg tactactgga ttcgaaaata
     4741 ttgatgaact taacaaagtt cttgttaaaa cagctagtcc atggactgat aaagtatagt
     4801 aaaaacataa aacggaggat attgttatga acagagaaga gatgactctc ttagggtttg
     4861 aaattgttgc ttatgctgga gatgctcgct ctaaactttt agaagcgctt aaagcggctg
     4921 aaaatggtga tttcgctaag gcagatagtc ttgtagtaga agcaggaagc tgtattgcag
     4981 aggctcacaa ttctcagaca gctatgttgg ctcgagaagc ttctggggag gaacttccat
     5041 acag
//
LOCUS       DAZKCY010000147         4940 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000147 DAZKCY010000000
VERSION     DAZKCY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.148"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..671)
                     /locus_tag="ACG8XC_002506"
     CDS             complement(102..671)
                     /locus_tag="ACG8XC_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9192172.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(745..1068)
                     /locus_tag="ACG8XC_002507"
     CDS             complement(745..1068)
                     /locus_tag="ACG8XC_002507"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192173.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVITDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(1209..2033)
                     /locus_tag="ACG8XC_002508"
     CDS             complement(1209..2033)
                     /locus_tag="ACG8XC_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192174.1"
                     /translation="MRWQKLLSKNAQNLFSLINLKKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFLHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKYFTKNGFVT"
     gene            2360..3235
                     /locus_tag="ACG8XC_002509"
     CDS             2360..3235
                     /locus_tag="ACG8XC_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9192175.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            3249..3572
                     /locus_tag="ACG8XC_002510"
     CDS             3249..3572
                     /locus_tag="ACG8XC_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192176.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            3787..4320
                     /locus_tag="ACG8XC_002511"
     CDS             3787..4320
                     /locus_tag="ACG8XC_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFP9192177.1"
                     /translation="MNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLYREYDYQ
                     EEECSPLFCFWGSLSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDPGHTYFA
                     SLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFVEKQLMV
                     QEKDKICSLPGGEWIDD"
     gene            4310..>4940
                     /locus_tag="ACG8XC_002512"
     CDS             4310..>4940
                     /locus_tag="ACG8XC_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192178.1"
                     /translation="MTIKREKWLRGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSILWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYWYAPFLSLMAYFMMLTVFRKKDLLYEMIAV
                     VTVLFVYLAARQFFSIRLLSGNTRFQSLTTVLLICFLTFSAILPLYLPKV"
BASE COUNT         1393 a          978 c          897 g         1672 t
ORIGIN      
        1 tttttaaatc tagcgaaaaa cccttatctt aaaggcgttc gttcaattct ttggcaaggt
       61 cttcaaagcc tggcttgcct agaagtgcga acatggtttt cttatagctc atgtagaaaa
      121 attttcttga tgatgatttt atccatcaat tgagattgac tgttttgctc tcctatgatt
      181 tcagctatga ttgtccgatt ttgcggtttt cttcttaact ggtagttgat tttcccagtt
      241 tccatcaact cgctaagcat atccacgact ccgccaaatc cggtcttttt cgtaataaaa
      301 cctagtgcta ccattcgttc atctagattt tttgctgtaa tcgtcacacc agtaatgagc
      361 gagtgctgta atgtttgggc cagctcttcc atcccttcag cattggatac gacggaagct
      421 gttttgttaa taggttcttc gtttatttct tttggttctt tcctctccac tttcactgtt
      481 tgacgaagga catctagttc ttgcaagagc gtttcgtatt gttcttgggt cagcatcacg
      541 ccagctactt tttcacgatt aaatatatat acgccagttc ccgcgtctcg cgcttgagca
      601 aatacatcca tcggcgatcg tttcacttct gtgatcgaag atgtgggaac ttctaatttc
      661 tttaattcca atggatttcc tcctttacta atcgcttttt attatagcac agactgactt
      721 gttttgatct tttttccaaa ggttttatcg gtttttgcct aaccagtaat tgatatcggt
      781 aagaagtctt tcgataccaa atgaaggatg gtacagatac gttttttgat cttcgttatg
      841 gtctagtgga aaaaaatgat cggtgatcac tagatctgca taccgaggcg gcttatttcg
      901 agtacataca tgtatagaga tccgttttcg atgtcttagc gttttgttca gtaccccagc
      961 aagtcttctt gcttcttggt gttcatagcg aaaaaaaaag acgattttca cttcaggagg
     1021 gtggacatct aaataccctc gttcctggag tgttttcagt aaaagcatcc cccactctac
     1081 tgcggggtgt gcaagtgctt gttggatcgc tagccgaaat tgatatagtt tatctttgga
     1141 attgggaaat ttttctaatg tttcctgata ataacgaact tcttctatca gccggtcatg
     1201 gataccaatt acgtaacgaa gccattcttg gtaaagtact ttgtcagctt tgatttgctt
     1261 tcgctctcaa attcgctatt cagccaaaac agcgaatcaa gcttttcagt cacaatcgtc
     1321 aatttccgat agtcttgtgt ctcttctatc cgtttccacc gcttttcagt aatctggtta
     1381 cagctcaagc gcaaaattgc tagataattt ttttctttta ttggcaaaat ctgctcctct
     1441 ctaaatagca attcaggaaa caattcaaag aatagtgttc gactgtctcc taaaatctcg
     1501 atttctgccg gatcctttct ttgcttcaat aattggatga acatctcaga aaaggactgc
     1561 tctccatcat ctgcaaaaaa atgcagaaaa tctttcaaaa agcaaaacag cagatatctt
     1621 acttttcttt ctgaaccaaa gatctgtgct tttttataag aaaaagaaag gtggtactcg
     1681 ccaaaccact ttctcaatcg ctcttgcgta gaacgatcgt acttctgctc gtaaggttca
     1741 ttcaatagca gatttttcag taaacaaaac gggggtgatt ggatcagaaa aacaaacaag
     1801 agataaggtt cgctatatcc ttgatcataa actaactgaa cggttttctt atgaagaatc
     1861 agctgaatgg gacagtcgct ttccgccaaa cgtttcagat gccgatccac tgttttaaca
     1921 tcgattttca gttcttcagc taaatcagct tttcgccaga tcaaatcttt ctttagattg
     1981 attagtgaaa ataaattctg tgcatttttt gataatagtt tttgccatct catcgatcat
     2041 ttctcacctg atttctaaac atattgtttg atcaaaatgg gttcactcct aaaaagataa
     2101 aaaactccct ttctatttat ttaaatacgc aaatcttcct cattattttt tattttactc
     2161 aatttaatcg tattctcatt tcaaaacgaa tgtactttaa aaaaacataa taagatcact
     2221 ttgcttgtag gatgtattcc cttccattac aatgaaataa atagcgattt tatgagaagg
     2281 tgatgtgaaa atggaagaaa aaatagaaga atccaaacaa aaaatcgatc agttgatata
     2341 agaattagaa aaagcggtca tcggcaaacg ttcaactatc caattgatga tcgtggccct
     2401 attagcaggt ggtcatgtct tattcgaaga tattcccggt gtaggtaaaa cgttactggt
     2461 caaagcacta gcaaaagctg tacagggaac cttcagcaga gtccaatgta ctcctgatct
     2521 gctgcctagt gatattcttg gtttttccgt atacaattca caatcaaaag agtttgaatt
     2581 tcgtccggga ccgatcttta ccacgatctt gttggcagat gagatcaatc gtacgactcc
     2641 tcgtactcag tcagcacttt tagaagcaat ggctgagaat catgcaacga tcgacaacaa
     2701 tacataccct ctagacaatc atttttttgt cttagctacg caaaatccaa tcgagtacga
     2761 aggaacatat cctttgccgg aagcacagct cgatcgtttt ttattccgac tacaaatcgg
     2821 ttatccttct tttgatgacg aacttttgct acttttggat aaattcgaaa aagtgttgga
     2881 taacttaaat gaagtattga acagttatga aatagaagaa ataaaaacaa atgtggcgaa
     2941 tgtctttgtc agtcccgaag tggcatctta tgccttgcag ttagtgtcag caacaagaag
     3001 tcatgctgcc atccagcttg gcatcagtcc acgaggaagc ctttccttta ttcaggcagc
     3061 aaaagcttat gctttaatcc atgggagaaa ttacgtcacg cccaaagatt tgcaggattt
     3121 agttccttac gtgttttccc atcgcctgat tttttacgat cgttcattga aggaagacga
     3181 aaagaaacaa atcattcata ctcttgttgc acaaattcct attcctgtga ggtagattga
     3241 ggtagacaat ggaaaaaatc aaagattatt tcattaattt tgtgttattt ctggtatatt
     3301 tcatcattgt gctttttgct cttgtattca acaattcttt aggctggttc ttgttggcct
     3361 tttttactct ttatgtatca tggagcttga tttcattaag cagctttcta aagaaacttt
     3421 cggttacttg tacattgcag ccactcgttt atcgcaaaaa aagtatgcag gttatttttt
     3481 ccatagcaaa aaagagaact ttttctttac ctctccctcg tttacaaatc ggcttcccag
     3541 caatctttgc agaaaaaaac aagagatttt gattcttaca aataaaccgc aagaagtcca
     3601 attgctttgg cagccaaaag aaagaggatg ccatgaaaca ttaactgtca tttttacagc
     3661 ctatgatagt ctaggacttt tcagaaaaag tgcaaaaaga gagatctctt ttcctgtgac
     3721 tgttctgcct gctttttgta agattcaagc agaaaaatta cagagagttc tggaaaaaaa
     3781 cagcagctga acgcgtacca aagaggtctt gattttcggg aacatcgcgc ttatcgtcca
     3841 ggtgattctt tcaaccgaat cgattggaaa ttgtctgctc atactcaaga gtggttatac
     3901 cgtgaatacg attatcaaga agaagaatgt tctcctcttt tttgcttttg gggttcgctc
     3961 agtccaaaat ttgaacctct gctagacttg tatttttctt tatggcattt gagaaaagaa
     4021 aagcagccgg aacttctgat attaggcaac cgcgcctttc atggcaaaga ccccggtcat
     4081 acctattttg cttctttgga agctggcgat gagaaagctt ttttcgcaca cttgaaaaaa
     4141 tatgcaaaaa aacaaatcgt cctctttgtt cctgagaatt ctcctgaagt ctcagaagct
     4201 attgaaacat tgtcaaaaga tcgaacggtt tatcttgttt atttcgtaga aaaacaactc
     4261 atggtccaag aaaaagacaa gatatgttca ttgccaggag gtgaatggat tgacgattaa
     4321 acgggaaaaa tggctgcgtg gcgtttactc ctttgtgata ctaagtgtag gtgctccgat
     4381 atttgtagta gctaatcaat tgcaaaacag ctaccttctt atcctaacta ttggtgtcct
     4441 atgccttatt gtctgctgta ttcctaaaaa atgggtacgt ttgctgcttt ctttgccagt
     4501 aatgataggt tgtttgtttt tatattttcc ttcgaagaca ttttccatat tatggatctt
     4561 acagtttttt tcgtcagtca cagaggaatt cttccatcta tcccaaggta aactttatta
     4621 tttgccagaa aagacagctt tttttctcat aatgttgctg atctatttat ttattacatt
     4681 gaccgtggat tacgactatt ggtacgcacc ctttctttct ttgatggctt acttcatgat
     4741 gcttaccgtt tttcggaaaa aagatttact gtacgaaatg attgcggtag taactgtttt
     4801 gttcgtgtat ctggcagcaa ggcaattttt ctccatccgg ttgttgtcgg gaaacactcg
     4861 attccagtca ttgactacag ttctgcttat ttgtttctta acttttagcg ctatcttgcc
     4921 tttatatctt ccgaaagtac
//
LOCUS       DAZKCY010000148         4915 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000148 DAZKCY010000000
VERSION     DAZKCY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.149"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(341..1240)
                     /locus_tag="ACG8XC_002513"
     CDS             complement(341..1240)
                     /locus_tag="ACG8XC_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP9192179.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
     gene            complement(1261..1533)
                     /locus_tag="ACG8XC_002514"
     CDS             complement(1261..1533)
                     /locus_tag="ACG8XC_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HFP9192180.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            complement(1556..2962)
                     /gene="lacG"
                     /locus_tag="ACG8XC_002515"
     CDS             complement(1556..2962)
                     /gene="lacG"
                     /locus_tag="ACG8XC_002515"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFP9192181.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            complement(3104..4810)
                     /locus_tag="ACG8XC_002516"
     CDS             complement(3104..4810)
                     /locus_tag="ACG8XC_002516"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFP9192182.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTVLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            complement(4810..>4915)
                     /locus_tag="ACG8XC_002517"
     CDS             complement(4810..>4915)
                     /locus_tag="ACG8XC_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9192183.1"
                     /translation="YSVTMMHGQDHLMTTVLLKDVIHHLIELYKRGAK"
BASE COUNT         1573 a          980 c          879 g         1483 t
ORIGIN      
        1 cgctatattt aagatttcaa acaactatat taataggaag aaaatttcat ttttcctctt
       61 agttctataa aaagtgtggc taatttgagg caaaatatta aattaaattt gccacaaatt
      121 aatggctcca ctattatttt aaatcaagaa gtagtaaata atcttatttt ttactaccag
      181 actgtcttta gatattagct tttcgtcacc cactacagtt gacatagacc cacaaaaaac
      241 ttaaaaacct tgcgatgtaa gactggcacg gagaagacca atctatcatc acaaggtaag
      301 agtattcaaa tcttattgaa gactcaaata ataacaaatt ttaattaagg atagtaatat
      361 catagatttt tctagaagtt tcttgaggtg aaattttcgt tacctgtctt ttatcttcta
      421 agaaattacc ttcttcaagt gaagtggaga gaccactcca aggttccaaa gcaatgaatg
      481 gacttttatt agttgtagac cacagaatca agttcggaaa atcatcaaaa tctactcgaa
      541 gacctttacc agacttcctt gaccgaagcg ttacgctacg agacttgagc tggtcaagtg
      601 taatggcatc atgggaaaaa agtgagtagt ccaaatcgag aattttttga ttttccaaaa
      661 aaggtgtccg atcttgcaaa tctaataatc ctgtctcagg aaaactcttt ggtatcgagc
      721 aagactcaac ctcactaaat tcaatataat aatcctcata gttttcacct tctactaaag
      781 gactattgaa tgctggatgg gctccaataa agtaaggcat tgttttttcc atttccaagt
      841 tcgttacctg aaattcggtt ctaattgatt taccattcaa ggtataaaca actctcagtt
      901 caaactgata taggtaatta tcaagcatct cagcatttgg tttaatacta aaggtcacgc
      961 tattatcatt gacttcttcc agaataaatt cttcttttcg aacaaaacca tgtctacgaa
     1021 taagacctgt aaaaaaaggt ctttcttgag gtctataaat agcccaatca tttcgtaaac
     1081 taccacagat aggaaataga ataggcgcct gtccattcca ataatttgga tcagcttgcc
     1141 agagatactc tatcccatcc ttgtctttaa tcgaagtcag ttgaccacca agtgttttaa
     1201 actgaacagt aagatactcg ttttttaatt cgatagtcac aactaattcc tttcttttta
     1261 ttattggaaa ggcaaataat ttacctcgaa gtgaatagtc tcacgtattt ctacatcgag
     1321 tgattgtaaa aattcccatt caaatgctga taactctaaa tcagaatggc aaatccactc
     1381 ttttccagtt gaaacatacc atttaccaca ttttagcaac ggggtagctt caagctcagg
     1441 aatcatagga caatcttcga aactaacaat atataccttt tcaaagcctg gtctaaataa
     1501 gtgttgtact ttttctaaag tatcggataa caatttttta cattttcaag taatattact
     1561 ctatcacttg agtttctgct actttcttat accaatgagc tgatttctta ggatagcgtt
     1621 cttgcgtgtc aaagtctaca tagaatagtc cataacgttt ttcgtatccg tttgaccatg
     1681 agaagacatc cataagtgac cagatgaagt aacctttaac atttgcacca tccgcaattg
     1741 cgtctgataa aacttccaag tgtttcttca cgtaatcaat acgaccatca tcataaacgg
     1801 tattatctac aaactcatct ttgtatccaa gaccgttttc agtgatgtaa atcttcttgt
     1861 agtttggata atcatttttc actcgcatga tttggtcata caagccttca ggataaataa
     1921 tccagtccca atccgttcta ggcacataat ctggtgcgat tcggcgacca acacctttaa
     1981 tctggtactt agaacttcct ttttcaccct taccattgtg aatgatttca gtctcaccat
     2041 caaaggcttg catccaatca ctcatgtagt agttgatacc aaggaaatcg ttcaaatctt
     2101 tagctgcttc aagtgcttgg aagtcttcat cacgaagatc aagttctcca ccattttcag
     2161 ctaggatgtg gttgacccct tccatcgttt tatctgaata gtgtccaagg taggttgcat
     2221 ccaagataaa tttgttatgg atgatatctt ctaactcagc ggcacgaaca tctgctggat
     2281 tttctggatc ataagggtat ttggttggca acgcatgtac tacaccaatt tcgcctttat
     2341 aacctttatc tttatacaat ttcacagcac gtgcatgaga aaccatcata ttgtggtgtg
     2401 attggaagac tttggcaaga tcatattgaa tgcctggagg gaatttacca accaagtatt
     2461 gaccatctcc aattgggcca atttcattga aagttgtcca gtagtttact tctgggaact
     2521 cttcaaaaca gaaagcggca taatctacaa agtgttctat attttcgcgg tttaagaaat
     2581 ctccatctga gtgaagaact tctggtgtgt caaagtggtg caaagtcacg aatggctcaa
     2641 catgacgttt gtggcactcg gcaaaaagat tatggtagaa ttcaacacct ttttcattta
     2701 cttcgccata gccagttggg aaaatacgag accaggcaat tgaaatacga ataccattaa
     2761 cgccatattt ttcagctaat tctaaatcaa caggatattt atggtaaaaa tcacttgcag
     2821 gctctgctgt gtaccaataa ttgtcttcaa gatacttatc ccatgcaact gggcctttac
     2881 catccgtgtg agtcgcacct tctgcttgat aagcagctgt agcaccacca aaaataaaat
     2941 ctttaggaag tgttttagtc ataactataa atcctttcaa aaaaagcatt tgagacgata
     3001 ttctgagttc cggaaaccaa ggaaatgaaa tgcaatgata tggttaacaa tattataagg
     3061 aactgaaaga ataatctcgt ctcaaataac ttttacaaat ttattaatca aactgttgtt
     3121 gaacaaatgc aagagcacct tgtccatcac gtgtcaattt gatatattga gctccttcag
     3181 tttttgcaag tttaatgccc aatttatccg tttcagcctt catatcatcg aagtttgaag
     3241 cgacctgtgg agccaagata accaagtcaa actctggcaa catttcacgg tgagcaccgt
     3301 aactacccgc tgccgctttg actggaacat tgtattctgc tgctgctttg ttcaaggcat
     3361 tggcaagcaa gccacttgta cctccacctg cacaaagaac aaggacatta gtttcttttg
     3421 tgatattatt gtttgctgct acatcttctt tagcaacgcc tgcttttcca agaacggcat
     3481 ctgctttagc agtattgaag tttgcagcta ctttttcttt aagagcatcg tttgttttac
     3541 cagaacgttc ttcttcaaga atttgttcat cgtatacctt aacaaatggg taataaatga
     3601 tagtatcaac aactaccaag agtccggcta agataaatga tagaacttgg aagttagtac
     3661 cgagtacaat tcccaacgga ccaggagtaa cccatggaag gttggcagag aatgagttca
     3721 tgttcaatgt gtcaacaaag aatttaaaga tccaaacgtt gacaatcgga gcgaaaatga
     3781 acggtacaaa gaaaatcgga ttcaatacga ttggtgcacc aaataagatt ggctcattaa
     3841 ccccaaagaa tgttggaaca actgaggcac gtccgatggc acggttacgt tctgatttac
     3901 aaatccacat gaacaagaat ggaacaatca atgtagctcc tgttccaccc atggtaacaa
     3961 taaacatttg agtaccagaa gtgataactt tatctgcatg ttgtccagct tggataagat
     4021 gcaagttggc atcgatattg gcataagtga tagcagcgat tgctggttcg acaattgaag
     4081 gaccgtggat accaacaaac cagaagaagg cataagcacc aaagataagt gtaattccaa
     4141 ggtaaccatc tgcagctgag aagagaggag caagaacggt accgattgat tctgcaacgg
     4201 ttacaccaag agttccctta acaatgagtt caaatccata aaggagaaca accgctacag
     4261 tgaatggaat caagtcttta aatacttgag agatatttgg tggaacctct tcaggcatac
     4321 gaatagtaac attatttttc acacagacct tgtaaacatt aactgtcaca aaggctgcaa
     4381 taaatgctgt caaaagacct tttgttcccg tgaaggctgt caagaagcct ccttcttttg
     4441 caggctcagc tgccatcaaa aggaacccta ccatagacgc tagcatcgta gataagaagt
     4501 taatctgatt ggtcgcaggt aggtcacggt tcattgagtc cgtcaaagct ttagcggtag
     4561 taccaccaac aaagaatgct aggataccca tcgaatagct ataaggagtc atcaagaagg
     4621 tctcaatgtc ttttgaccag tggaatcccc aagcatttgg gacataggca ataaggataa
     4681 agatagatga gaaaaggatg actggcatac cagcgataaa tccatcacga atagcacgaa
     4741 gatagatgtt tcgagagatt ttctcaaaga acggtttccc tttctcaata agttcaatga
     4801 gtttatgcat tattttgctc ctcttttata aagttcgatg aggtgatgaa tcacatcttt
     4861 taataagaca gtagtcatta agtggtcctg accatgcatc atggtaacgc tgtat
//
LOCUS       DAZKCY010000149         4814 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000149 DAZKCY010000000
VERSION     DAZKCY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.150"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            682..1356
                     /locus_tag="ACG8XC_002518"
     CDS             682..1356
                     /locus_tag="ACG8XC_002518"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9192184.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1353..1694
                     /locus_tag="ACG8XC_002519"
     CDS             1353..1694
                     /locus_tag="ACG8XC_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192185.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLI
                     AVTKEKQIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTKEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            1724..2083
                     /locus_tag="ACG8XC_002520"
     CDS             1724..2083
                     /locus_tag="ACG8XC_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP9192186.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            2343..2465
                     /locus_tag="ACG8XC_002521"
     CDS             2343..2465
                     /locus_tag="ACG8XC_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192187.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEKKKKE"
     gene            2607..4691
                     /locus_tag="ACG8XC_002522"
     CDS             2607..4691
                     /locus_tag="ACG8XC_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9192188.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
BASE COUNT         1629 a          773 c         1037 g         1375 t
ORIGIN      
        1 aaaagaactg cgaactctcg cagttctttt ttttaaatac gggataatgg aagtttccag
       61 cagacaagag aagaaagcaa aagaaattgc tatttgattc acaaggttct ataacgaaat
      121 cgcaatcaat caaacattta caaagaaggt aattcaaatg gaaatctgat ttgatgatgc
      181 ttctgctgtt gaaataaagc agcatctagc acctgaaaac aaactaatag taacattcga
      241 agatggagta ggtccgtatt ttcagcatgc aataatccat atgtaagttc aattcaccct
      301 tgaaatcatc ggaccagata aagttatgac caatctatcc caagcaatct agaagagata
      361 ttagtaaagg gacatttact agaagtctta gatgagccta taaatatatg tttcgatggc
      421 gtcatcagtg gtctgattga cggaagcttg ggtgtgatcg atctctctgg accaaacaac
      481 gaaggaatca aggggaatcc ggcacgttaa gaaaaaaata gcataaaaaa ctagacactt
      541 gatgagcgtt cattcaattg tttgaaggaa ctaggctggt gtcatttttt atacatatat
      601 tttctctttc gtcttgatag ggcttatggg ataatataag gaagaaaatc atcgttttat
      661 tcaaaaagga gtttttgaca aatgaaaaaa cgtatattat ttatcggtga tagcattaca
      721 gacgcaggaa gataccgagc agaagcaact gatctaggta aaggttatcc acttttgatt
      781 gccggaaaac taatctccca atatccaacg atggaatttg aatgcatgaa ccgtgggata
      841 ggaggagaca aaattgcaga tctgcaagta cgctggcaag aagattgttt agctttacaa
      901 cctgactatg tatcgatttt gatcggaatc aatgatacct ggcataatac tagcgatcag
      961 gctatattcg gaactgagaa atctgctcag caatttgaat ccgtttatcg aaaattacta
     1021 aatgaacttc aaaaagcggg aatctgcaac atcatcttga tggagccgtt tgtacttcct
     1081 ataaccgacg accgtcatgg ctggcgtaaa gacttagacc ctaaaatcca aattgttcgt
     1141 aaacttgcta aggaatatgg cgcattgctg gttcctttag atggtatatt caatgcttta
     1201 gggattgctt atggtttttc gaaatataca agagaagatg gggtccaccc aacgattggc
     1261 ggacatgaga tcattgcaca aagctggata gatgcaattg ccgcagtcga tttttttagt
     1321 ggagataaaa atgagcgaaa gaaaagtgaa aaatgatgga aataaggtgg gcagtaaaag
     1381 aagatttacc tcaaatcaat cagctgatta ctgatcatcc agaatcattc gtaaaagcaa
     1441 aaacggcagc tgacctaaat gataagaata aactattgct cattgctgta acaaaagaaa
     1501 aacagattat cggatttgcg caagcggacg tgcaacagtc agaagaacga ttggatcagc
     1561 gaccatcagg aaaactaatc caacacacat tagtaacaaa agaaaatacg aaagaaattc
     1621 aaaaaaaatt tgcagaatac ttgatgctgg aagctaaaaa aagaagaata aaatcaattt
     1681 ttgcccagtt ttaatcaaaa acagtaatag aaggagaaat acaatgagtt gtttaggaaa
     1741 tgtcatctgg tttatattcg gcggactaat cggtggtctt agctggttag cagcggggat
     1801 actttggtgt atcacgatca taggtatccc aatcggtatg caatgtttta agatcgcctc
     1861 cctttctttt gcgccttttg gcaaagaagt aatttatcac cagtctggca tgtcattgat
     1921 cgttaatatc atctggctga tcgtttcagg cctaccaatt gctctcgcac atgtcgtcag
     1981 cggcatcctt ctatgtatca cgatcatagg gatacctttt gccaagcaat ctttcaaact
     2041 tgctcgattg gcgttgatgc cattcggtgc agaaattgta taaaagagaa taataaaggt
     2101 tagataacgc atagagtcag gtgggataca tttatttcac ctgactctat gcgttttcct
     2161 gtataagttc ttccatgtga cttttacgtc ttttatttat atgggattac tgtttatgga
     2221 aagttgaaat aattttcatt aaagaactct ttctatattt ttgaaagcgt agtggagctt
     2281 cgaatatctg ggaaggacat tttgcaggga ttgaaaaaag atattgtcct atcgaaaaat
     2341 taatgaattt agacaaattg ccagcaacag gcttcaaact tagttgctat cctgtcaaga
     2401 tcaagaaggc aagtgcgggt tggataagag cagtggcaat gatcgaaaag aaaaagaaag
     2461 aataagccaa aacatctgta tggaagaaat tttccgatgc agatgttttt cattttttct
     2521 ttcggatacc ttgacatatg aataaccatt catttataat tagttatgaa gagatattca
     2581 tatgttgagg cgaggaggtc attaaaatga aaagttacag attagaaggt ttagattgtg
     2641 cgaactgtgc aatgaaaatc gaaaaaggag ttcagaaaat caatggagtc aaggaagcaa
     2701 cggtaaactt cacttctgga aaactaacga ttgacgcaga ggaagatcat ttggctacga
     2761 ttgaacaaga aacaaaaaaa gtagtgaagg aattagaacc agatgtcaaa gtaacagaaa
     2821 tagataagga aaaagtatct gaacatggaa atgaaaaaaa gagaaatacg ctgttccgga
     2881 ttttattttc ccttgcaggt atcgctttat tgctattatt tgattttaat gaaccaattc
     2941 gcttgatagg gtatttattg atttatctgc tgataggata tgatgtagtg aagaaggcag
     3001 tgatgaatat cgtcaaagga aaaatctttg atgagaattt tcttatgtct gttgctacga
     3061 ttggcgctat gttgatcggt gaatatccag aagccgtagc agtgatgcta ttttatcaaa
     3121 tcggcgagta tttccaagga ttggcagtca gtcattcaag aaaatcgatt cgcgaattga
     3181 tggcgatccg tccagaaaca gctcacgtgc agacggcgga aggattgatg accgtgaacc
     3241 ctgaagacgt gctgatcggt cagtttgtac ttgtgaagcc tggtgaacgt gtaccgttag
     3301 atggaacgat catcgaagga gaatcattag tcgatacgtc agcacttaca ggagaatcag
     3361 tccctaaatc tgtatatgtt ggtgaaacag tattaagcgg atttatcaat aaaaataaac
     3421 catttttagt tcaagtagaa aaatcttatg aaaattctac tatcagcaaa ttattagaat
     3481 tagtagaaaa tgccagcagt aaaaaagcac cagctgaaaa tttcattact aaattcgcgc
     3541 gctattacac accagtcgtt gtaggattgg ccgttttatt agctgtttta cctccacttg
     3601 ttgtttctag agcggcgttt tctgaatgga tctatcgagc attgacattt ttagtgatct
     3661 catgtccatg tgcattagtg atttccgtgc cacttagttt ctttggcggt atcggaggag
     3721 caagtaaaat cggtgtatta gtaaaaggtt caaattactt ggagttgctt gcacaaactg
     3781 agacagtggt attcgataaa actggcacat taacgaaagg tgacttttcg atccagacga
     3841 tcgatccaac aggaattgat ccaaagaaat ttatgcaata tgttgcaagt gcagaacagt
     3901 tttctacaca tccgattgct caatctgttt tagaaggcta cgcaggtccg cttttaccaa
     3961 cagctaatat ccaggaattt gcaggtgaag ggatcttagc tgaagttgat ggcaaacaag
     4021 tattagtagg taatcacaaa ttgatggaaa gatttgaaat ctcttttcct tcaagtcaag
     4081 aaatcggtac attgctttat ctagcgatcg atcaaagtta cagtggttat cttgttattg
     4141 cagatacctt gaaagaagat gccgtagacg cattggttca attaaaacag gctggtgtga
     4201 aaaacacagt catgttgacg ggcgactcga aaaaaattgc tgatcatatc gggaagcaag
     4261 taggcgtgga caagatatat agcgaattgc ttccagaaga taaagtgcag cggttagagg
     4321 aaatcttaca aagtaacaat aaaaagaccg catttgtcgg agatggaatc aacgatgcac
     4381 cggtgctagc aagagcagat gtaggaatcg caatgggtgg attgggaagc gatgctgcaa
     4441 ttgaagcagc agatgtagtc atcatgaatg atcaaccatc gaagattgca gaagcaatcc
     4501 atttagcaaa gaaaacattg aaaatcgtca aacagaacat tgtctttgct attgggataa
     4561 aaattttggt actttcctta ggtgcttttg gttttgcatc aatgggagat gcagtatttg
     4621 cagatgttgg ggtgacagta ttagctgttt tgaatgccat gcgaagcttg catgtaaaaa
     4681 ataaacgttg atcaatgaag aggacgaatg aacgtcctct tttttttata tccttttctt
     4741 aatgaaaaat gtaaagaaaa tagataggat tataaaataa ctaatgtttt taattttgca
     4801 gaaaattcaa aata
//
LOCUS       DAZKCY010000150         4812 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000150 DAZKCY010000000
VERSION     DAZKCY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.151"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1204)
                     /gene="nagA"
                     /locus_tag="ACG8XC_002523"
     CDS             complement(56..1204)
                     /gene="nagA"
                     /locus_tag="ACG8XC_002523"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9192189.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1280..2116)
                     /locus_tag="ACG8XC_002524"
     CDS             complement(1280..2116)
                     /locus_tag="ACG8XC_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9192190.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2300..3517)
                     /locus_tag="ACG8XC_002525"
     CDS             complement(2300..3517)
                     /locus_tag="ACG8XC_002525"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9192191.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3666..3827)
                     /locus_tag="ACG8XC_002526"
     CDS             complement(3666..3827)
                     /locus_tag="ACG8XC_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192192.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3900..3983
                     /locus_tag="ACG8XC_002527"
     tRNA            3900..3983
                     /locus_tag="ACG8XC_002527"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3934..3936,aa:Leu,seq:gag)
     gene            complement(4223..>4812)
                     /locus_tag="ACG8XC_002528"
     CDS             complement(4223..>4812)
                     /locus_tag="ACG8XC_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192193.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1466 a         1001 c          775 g         1570 t
ORIGIN      
        1 aaaaacctta aaaccaaact taagaagttt agttttaagg ttaacttaaa ttatattatg
       61 cgtggaaacg ttccacgcca tctagataag ttgcttccaa tgtcatatca gggttcaata
      121 cgataaagtc tgcatcgcgt ccttgagcga tttttccaca tgtatcatcg attttacagc
      181 tgattgctgg aatcagacta gccatcatga ctgcttgttc tggtgttgct aatccccagt
      241 ctacaacatt tttaattgct tctttcaatt tcaatatact tccagctaag ttgccagatt
      301 ctagacgtgc tgttccttct tttactacta ctggaaattc gcctaaattg taatttccat
      361 caggcatccc accggccatc atacagtctg taattaatgc gacatgatca tgtcctgctt
      421 tttccattag gattcccgca gcatttggat gtacatgatg gccatcacaa atcaattcag
      481 aaaagacatg ttctaaagat aataacgcac ccaccatacc tggttcgcga tgattcaatc
      541 cacgcatacc attgaaagca tggacaaata cacttgcgcc tgcttcaaca gctttttgcg
      601 cttcttctag tgtagcatta ctgtgtccta gagaaacaac tactccttct tcagtcactt
      661 gtttgacaaa ctcttctaca ccttgacgtt ccggtgctaa agcaattttt ttgattaaac
      721 cacctgaagc ttcttgccat tcatggaaag tatccaaatc aggatccccg aaataacttg
      781 ggttttgtgc ccctttgtgt tcttctgtga agaaaggacc ttcaaaatag attcctttga
      841 ttttagctcc tggtgcttcc tgatacattt tgccgattgt ctcagctaca tcttttaaac
      901 gttctttgct tgacgtcaat gttgttggta aaaatgaagt cactccacaa gataacagac
      961 cttcagacat cactttgatg ccttctgcat cattatccat tacatcatga ttcatatagc
     1021 cgtgaatatg tgtatctacc aaaccaggag caatccattt gccgctttgg tcaatgatgt
     1081 tgacttctcc ctcaggtagt tctttggtgt attcaccaaa aagtccatcg gtaatttcaa
     1141 gatagcctgc atgtttgaca cctgacgcta aaaagaattt gtcagcatag ataaaagttc
     1201 tcattctatt ctctcccttt gtttttctac ctctaaagta ctcttattta gagaagaagt
     1261 caagaatggt ctacaccatt tacaaatttt ttgaaatttg tttcacagct tgtgctaagc
     1321 cttcatagaa tgctgctctt tctggttctt ctttacgctt gtatattaag cttttaagca
     1381 caaataaatc attgaggtaa tagtagctat tcttctcttg agcccaatag attccttgat
     1441 tcacctgttc taacgcttcg tcgatttcat tttgatggaa taagaaattt ccataattat
     1501 aaaaaatttg cgaaaattca ctttttgatc gttcatcagc tgattgatgc agcagttgga
     1561 tgcttcgact caaatagtag cgtgcttcag ccgtttttcc tgccacacca tatatttttc
     1621 cgatacaact aatcagttgt atttctgtgt cagaaacatg gattctttct ttttggctag
     1681 agtagcttaa tgcttctttc aaataataaa gggactgaga gatattttga cgcaacgtga
     1741 attcgcaaac accgagataa taataatagc gttgaaagtc agttgcttga tacagatttt
     1801 cagaaatatc agaagatttt agtacttgtt ctaacttccg ataatcacgt ttttggaaac
     1861 aactatccaa cagttcaaat aatttatcgt ttgtccgagt agtttttcga gagagattca
     1921 tcacatgatc aatcgttacc cctagccgtt gacaaatctg catcatcacc aatacattcg
     1981 gcagttcctc attattttcg atccgactta atacactttg tgaacaaata tcatgcgaaa
     2041 gcattttttg cgtcattttt tgttcttttc gaatctcttt aatgatcgtt ccaaaggact
     2101 cgattaacgt ttccataagt atcctcccca aaatatgcgt attcgcattc tattcgtatt
     2161 ttactttagg aagtcgcaaa cttcaagaaa agacttgcaa ctaaataata tactttagtt
     2221 atttaagaaa actccactag aaagagagcc tcagtgtgtt tgtctgtggc cctcctactg
     2281 aaaaatttgg tttttaaaat catttttggc gataggttgc taggaattct ctcattttct
     2341 tagctgtttg tttcaaatca tccattttag gaagataaac gattcgaaaa tgatctggct
     2401 ggttccaatt aaatccacca ccatgtacaa gtagaatatg atgttcatgt aaaaagtcca
     2461 aaacgaattt ttcatcatcc aaaatattga atcgctttgt atcaattttt gggaatatat
     2521 aaaaagcagc ttttggtttt acggctgaaa gaccaggaat atcattgatt gcattataga
     2581 tatattctct ttgttcatag atccttccgc ctggcaggag cagctcatcg acactttgat
     2641 aaccgcctag cgcggtttgg ataatttgct gagacaatac attggaacac aaacgcatgg
     2701 aggacaacat attcagccct tctatatagc ctttgacgtg tgacttatct ccacttagga
     2761 ccatccagcc tactcgaaat cctgctacac gatgagattt ggatagaccg ttcagcgtaa
     2821 caacgaacaa atcaggcgct aatgtagcaa tcggaatatg ttctagacca tccatcacta
     2881 agcgatcata gatttcgtcg gaataaataa tcaggttatt ttgtcgagct acttcaacga
     2941 tttcttctaa taattctttt ggataaagtg ccccagtagg attattagga ttaatgataa
     3001 caatagcttt tgtccgactc gttatttttg atttgatatc atcaatatca ggataccatt
     3061 ctgcctcttc atcgcaaata taatgaacag gtgtgcctcc agctaatgca atagaagccg
     3121 tccacaacgg atagtccggc attggaacca gtacttcatc tccattgtcc agcaacccct
     3181 gcatacacat cgtgatcaat tcgctcactc cgttgccggt gtagatatca ttgatcgtca
     3241 cattcggaaa atgtttcagt tggcaatatt gctcaattgc ttttctagca gaaaagatcc
     3301 ccttggaatc tgagtagcct tctgagtcac gggcattcat gatcaaatca cgaatgactt
     3361 cgtttggtgc ttcaaatcca aaaggtgcgg gatttcccgt gttcaatttt aaaatactaa
     3421 tgccctcttc ttgcatccgc tccgcttctt ctagaacggg accgcgaaca tcgtaactga
     3481 caccttctaa tttgttggat ttttcaaatt ctctcatcac ttagccaatc cctcatttca
     3541 aaaagttaga actttccaaa ttttttgtta tttactattt caatatcata ctccatccta
     3601 gaaaattttg ccacttattt aggaaaaacg taagtattaa aaaaacacaa acaagcgttg
     3661 agaacttaat tgtcatgata gtcattccat agattcgctg cccaatccat tgcaattcca
     3721 caaatgattc ctagcataaa accaagcaaa aaacgtccga atgcaagtcc aatcaataca
     3781 cctaagatca ttcccagtcc tgaaaattct ccgtcatatt tctccatctt aataagtcct
     3841 cccatttttg agcttgacaa ctatgtaaaa cctcgctatc attagtgtgt tattattacg
     3901 cagatgtgtt ggaattggca gacaagcatg attgagggtc atgtgagcgt tagctcgtgt
     3961 gggttcgagt cccatcatct gcactacaac tcgtgaagtt aattttgctt cacgagtttt
     4021 tttgtttgaa cgtttcttta tgccattata tacatatcac tgtatccttt acaaaaagaa
     4081 cgtacaatca tgtaaacagc ctgaggtgag tatatgaagg tcggaaacaa gctactcgta
     4141 gaaacttgca agtgttaaaa caattaatcg aaaaacgagt aaaatccctc acctaatact
     4201 aaagcgaggg attttttgta tattatctgt ttatcaccgt tattataaag gtacaaatac
     4261 caagcacggt taatccaaat tgcataaatt catttttttg tacactcatg cttttcatta
     4321 gctctttaac tgccattagt actaagatgg cgataacaac ataaagtata tagtacgtcc
     4381 gcattccgga agcccatact gtgggagaaa atcccataat taaccttgaa caaaatccca
     4441 ttatcatcaa aataactaaa aaaatagaag ttttcttatt gtcaaaactt aaataaattc
     4501 caacaattaa acatagtaat aatgccaaga ctactaaagt agctatccaa gtacctggat
     4561 aaaaaagtga cagtttcgtt cctaactctg taaagagatt attcaggtta ctactactcc
     4621 aaatcattgc ccttttattt ccattgacgt aagtaaagct ttgacccatt gtattaccaa
     4681 aataaataat caggtttaag aaaaacggta atgcagtcaa tatacgcaca taatagtttt
     4741 gacatttcct atatgttaga aggaaaatta agaaaaatag caataaaaat aagatattca
     4801 tgtctaaaaa aa
//
LOCUS       DAZKCY010000151         4722 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000151 DAZKCY010000000
VERSION     DAZKCY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.153"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8XC_002529"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8XC_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP9192194.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACG8XC_002530"
     CDS             724..1878
                     /locus_tag="ACG8XC_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFP9192195.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACG8XC_002531"
     CDS             2093..3061
                     /locus_tag="ACG8XC_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP9192196.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8XC_002532"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8XC_002532"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP9192197.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACG8XC_002533"
     CDS             4091..4699
                     /locus_tag="ACG8XC_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFP9192198.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1456 a          907 c         1109 g         1250 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aa
//
LOCUS       DAZKCY010000152         4702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000152 DAZKCY010000000
VERSION     DAZKCY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.154"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..586)
                     /locus_tag="ACG8XC_002534"
     CDS             complement(<1..586)
                     /locus_tag="ACG8XC_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192199.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVF"
     gene            complement(734..1276)
                     /locus_tag="ACG8XC_002535"
     CDS             complement(734..1276)
                     /locus_tag="ACG8XC_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFP9192200.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1411..1800
                     /locus_tag="ACG8XC_002536"
     CDS             1411..1800
                     /locus_tag="ACG8XC_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192201.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1804..2568
                     /locus_tag="ACG8XC_002537"
     CDS             1804..2568
                     /locus_tag="ACG8XC_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9192202.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2697..3644
                     /locus_tag="ACG8XC_002538"
     CDS             2697..3644
                     /locus_tag="ACG8XC_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9192203.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3774..4514
                     /locus_tag="ACG8XC_002539"
     CDS             3774..4514
                     /locus_tag="ACG8XC_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192204.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1638 a          780 c          939 g         1345 t
ORIGIN      
        1 taaatacacc tagactgacg atgaagagca tatccaacag atttcctgcg acttggatcg
       61 tcgaagggtt ggtcagtggc attaaagcaa ggacaaaaaa ggaaaacaag ctgtaaataa
      121 aacaggtgac aggaactaat cgaaagaaga gcggacattt caattggcta aaaatgagtg
      181 aaaaaataaa ataagtaaaa atggataaaa tcacgactaa accattcatg ataaagaaga
      241 aaagcgtatt gtgattaaac gaataaaaaa gaattgctaa aaaagaaaac gttttaaatg
      301 caatcaatac gcttgaagct tcgtttaacg taccaggatg tcgatgaaaa aaataggcac
      361 cgaaataaac agaaaacagt aagccgcaaa gagaagacaa aatcaaaaaa aacggatttt
      421 cttcggcttc tgtgatatca gaatgatcca aaagcaacca gcctaatgca ctagaaggcg
      481 catgtgaggt cttaaggtat gaataacata gaaaaaataa gatcgtcaaa gttgcaaaaa
      541 aagagcctaa atgtaaaaaa ttagtggttc tacgttgaat ttccataata tctccaaatt
      601 aaattgataa gtatataaag tataataaca aattgatgag tatatgtcga tgttttttaa
      661 tcaaaataat aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt
      721 ttaagtttgt gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg
      781 aaatatattt tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc
      841 gatacagttc acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt
      901 ccagccccat tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca
      961 aacttttatc ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg
     1021 ccaatttgtc ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga
     1081 caaataaatt cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa
     1141 tcgtgatctc gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca
     1201 tgaaacgtaa cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca
     1261 tttttggatc aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa
     1321 tcgtaagtga agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat
     1381 agaattataa agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc
     1441 agccacgtgc taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca
     1501 attgaggtag agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt
     1561 tttttctttt tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat
     1621 ttttctattt atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc
     1681 ttatttcccg agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag
     1741 acaactgttg gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa
     1801 caattgaatc caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt
     1861 ctgaaagaat taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca
     1921 agtaaggagt tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc
     1981 ctgccagatg cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat
     2041 acattttggg atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta
     2101 gaaaaaagag gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg
     2161 acaacatgcg ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt
     2221 tcccctgcac ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat
     2281 gtcgtaccaa aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg
     2341 cagccggaaa agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa
     2401 atcccttata tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa
     2461 gaaattgttt ttcgttcctc aaaaacacag cgacatatgg tacaaatacc actaatggaa
     2521 gaagcttcca cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa
     2581 atcactcatg acgatagagg attttccgtt ttttattttg ggctataata aactctgtta
     2641 ggaaaatttg gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc
     2701 ccactttagc agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca
     2761 tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg
     2821 aactggatta ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca
     2881 tcaaattatt tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga
     2941 tgggaatcgc taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg
     3001 ccttcgacgt cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg
     3061 attggatcga ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg
     3121 attatgtaga cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa
     3181 gagattatca aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa
     3241 gggaaactgg ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt
     3301 ttccaaatca ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag
     3361 ataaaacttg ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta
     3421 taaatgctgg aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt
     3481 tagaccaaat cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg
     3541 aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc
     3601 gtcgtttttt tcctgaacta gttatcagag agacaacgaa ttaagtctta ttactatata
     3661 tatatttatg aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca
     3721 atgaatctgt gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag
     3781 aaatcagacg aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa
     3841 ccaacgacaa gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc
     3901 agtccccgtt tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc
     3961 gtcgcaagcg tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa
     4021 agcataaagg aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag
     4081 gacgaaaaaa actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag
     4141 agtccaagca tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt
     4201 ttgttcctaa atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg
     4261 aggatcattt aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag
     4321 aaccgacgcc gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa
     4381 caaaaacgtt agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta
     4441 tcttagaacg ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata
     4501 gctattttaa ataagtgata tacaaattta tattcggcta cagttggaaa ggaaacgctg
     4561 cggcgtttcc ttattttata ctctatccga aagtttatat cgtatcctca aatttttttg
     4621 agtttaagcg aattttttct cccaataagt aagaaaataa tggcaaaata aaaaaacaaa
     4681 ctattttcgc agatcgtttg ca
//
LOCUS       DAZKCY010000153         4660 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000153 DAZKCY010000000
VERSION     DAZKCY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.155"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..972)
                     /locus_tag="ACG8XC_002540"
     CDS             complement(<1..972)
                     /locus_tag="ACG8XC_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFP9192205.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRI"
     gene            complement(1043..1339)
                     /locus_tag="ACG8XC_002541"
     CDS             complement(1043..1339)
                     /locus_tag="ACG8XC_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192206.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(1332..1625)
                     /locus_tag="ACG8XC_002542"
     CDS             complement(1332..1625)
                     /locus_tag="ACG8XC_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFP9192207.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1771..2322)
                     /locus_tag="ACG8XC_002543"
     CDS             complement(1771..2322)
                     /locus_tag="ACG8XC_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFP9192208.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2496..2795)
                     /locus_tag="ACG8XC_002544"
     CDS             complement(2496..2795)
                     /locus_tag="ACG8XC_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFP9192209.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(2822..3130)
                     /locus_tag="ACG8XC_002545"
     CDS             complement(2822..3130)
                     /locus_tag="ACG8XC_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFP9192210.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(3247..4206)
                     /locus_tag="ACG8XC_002546"
     CDS             complement(3247..4206)
                     /locus_tag="ACG8XC_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9192211.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            4350..>4660
                     /locus_tag="ACG8XC_002547"
     CDS             4350..>4660
                     /locus_tag="ACG8XC_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9192212.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     L"
BASE COUNT         1351 a          887 c          726 g         1696 t
ORIGIN      
        1 aatacgttga gaaattcttc catctgtata aacacggaat tgttttccac cagccattgt
       61 tgtatttcca ttatcatccg ttacaatatt aattgaatag tagcttccat tttcatcttg
      121 cattagatct ttcatatatt catttgtgct aattaaatta gtatttgatg catttttatc
      181 aggagaatca tacaaccata aagctttatt atttagtaca gtttctttag cagcttccca
      241 acttgaaatt gctgagctac ttgtagattg tgtcgttgta gttgttgctt gttgagtaga
      301 ttcttctact actggagttt gaggagtttg tccattcaca atatcaatga aaccttgttt
      361 taactgttgg ttctgagttt cattgaagta catgtagttt ttatcattgc cttgattttg
      421 ttgcgttaaa taaactttag gttgtccatt ttgaaagcca aagaaatata catgctgccc
      481 taaatattct ccagtttcta catcagaata aactgctact aaattgaaat ctgcatgacc
      541 agttccatct tctgaccatt gtacggtaac tggtgcttca ttgattgcca ttttccaatt
      601 tccatcaata actgcacttg gtaaaggcgt accatacaga cttacattgt tctgaggatt
      661 gtagctttta tattcttgac ctaatgtctt tccccattgt gggacaaaaa ttgctagttg
      721 agtagatttc tcactatccc acaaagtatt tttaacctct tcagaagaag attcaatgat
      781 tgaactttca cttgtacttg cttccacaga ttgtttcatt tctgttgtag tttgttcagt
      841 tgattgcgtt ttactttttt tagcctcaat tgttgtatca gtttctacag tctctttagt
      901 cctcgtattc tcagatgtac aaccagtgaa taaaatcccc attccaatta gtaataacaa
      961 ccattttttc attttatttc cccctttcaa tttctccctc gcgtacttat tttatcagtc
     1021 aattaatctc aatggtgttc atttagttaa tcctttccca agaatgtcga tagccagcat
     1081 gacaacgatc tcttccttga gggctttcag atggattaaa actagtagaa atttcccttc
     1141 cacatcctct acagcgataa gttctcatta tttttgggct attagtatta tttttcttag
     1201 ttagagctat gagtccaact atgattaccc caactgtctt aattactttt cctttatcta
     1261 tattcggatg cgcaagcata tatttatata accaatatcc accgtaaatt aaaccaccta
     1321 atacaatcag cttagccatg tactcgtctc ctttcttttt gtttaattac tttttgaatc
     1381 catcttgcag agaaaccata aacttctgtc aattgacgga tatctacagt tttttcctgt
     1441 tctatcatgt tttcgattct ttttctaact aactcacgat cgtacatttt cattggaaaa
     1501 gaaatctgtt gccctcgaaa agcaagataa aattgataga gcaattctga atccagttcc
     1561 atttcctcaa aaaagtcatt catactttta taagagttac tcaaacaagt ttcatcaatc
     1621 tccatctaca caccccctgt tattttttac ataatgaaaa tagcatatta ctgggtacat
     1681 tgataattca tataacatgt atcatcacat gtatttttaa agtcaaaaag catgtaaaac
     1741 tacttataag taacataaga aaataaaatg ttatttttct tgaaataata attgactatc
     1801 tgccaattgc cattcattac gaacatgaat acttagtggg tgggataact taccttctgc
     1861 atttatcaat actttggtca tctttttttg tttcgcactc aattgatcgt aaatggtctt
     1921 cgcttgttca taagccactt gactcgtcgc tgtaatcgaa ccacaagcaa aaataatggt
     1981 gttgattttc ttttctgtga aaacagttgt taatatttcc aaagattgtt catcatttcc
     2041 acgaggaaat acccgaggac ttttcgcttt tgtatacgaa aaaagattca cagcaataaa
     2101 accagtaaat cctaattgtc ggatattctt ttcgattaat aacatactca aatcattcgt
     2161 aaatggttct gtatctgttg gacgaatagt aatgactacc gctaatttat cttccgcttc
     2221 ttttcctttg atcgcccatt gtcgtttgaa aacatagcga tgggtatgat gaggatctcg
     2281 ccaaacttct gtttgtgtgg tcactaattc tttttcaatc atctcttttt ctcctatgct
     2341 aatttttgtt caagcaattc aatcaccatt gccgttataa gtgtcaatac tagttgaaaa
     2401 tatttcatat tctttctcct tttttcttca gaatttgatg aatcgctgta aggatatctt
     2461 ctaaacattt cgcaatcatt ctcaaataat cgatcttatt cattcgttcc tccaatctga
     2521 ctccaaacca atcgtccttc taccagtaac ccacgaacaa attctcgctg gctggtaata
     2581 tctgtaatga aataaactac atagtctttt ccatcttctg gtgcgcaata aatataaaga
     2641 tgtgtcatca ctaaacgtgg tagagtaaca tcttcaggaa gcatacccat ttcaatttcc
     2701 tctttttcca tcgtaacgga ttcatagccc cactcttcag gagtagtgac tagctgtgca
     2761 cacttttcca gtacttcgat agccgtttct aacaagtcct ttcgttcctt tcttttagat
     2821 atcagtattc ttctggtaat aacatcgttt gatactcacc cgaatcaatg acccatattt
     2881 tttgagacac tgatttcaaa cgaagcttag gtgaaatcag atactctttt cttgcttctg
     2941 gttcttcttg acgatgaacc aacttttgat ttcctttttc cgtagaaaat tcaaagattt
     3001 gaagatagtc taaatgaaat tgcttttgaa cgttttgatc gattagattc caacataaag
     3061 cttgtaattc caccggcaaa ctgtccatta ctccctttgt gacataacgt tgtcctttat
     3121 tttgaaacat ttcttttccc tcctttgcca taaaaatagc ccaatcttct attaaagaag
     3181 attgggcttc atggatataa tatatatttg atattcaatt tgattcattt tatactatct
     3241 gatttgtcaa attaagctag acaatatgtc tttatctatg cagctcaaaa atacttccaa
     3301 tggtgttcga taatttaatg attttcttgg aatatggttt cttttcgagg caacggattg
     3361 aataaaggtt tcatccgtgt ccctaaagtc cattgtttta ggtaagccat ctcgtctaag
     3421 caagccgttt gagtgctcat ttagccctct ttgcgaaggt gttcctggat ctgcgaaaaa
     3481 gatggcaata tcgttcgagt tacttataga tcgccagttg gaaaattctt ttccacaatc
     3541 aaatgtcatg gatttaaaca agtgtttagg catggatttg aaccaatcat ttaacctgtt
     3601 ttcgatatct tcggctcgtc tgcctgtcgg tttcaaagta ataataacct tggataaaca
     3661 ctcaaccaat gtaataacag cgctcttgtg tttaactccc acaatggtgt cgacttcgag
     3721 atgaccaaat tctttagtca ggtgtggata gagactgttc cgttcatgaa tcgttctgcg
     3781 aaaggcctgt tttccacgct tttcttgatg attattcggt ttacgtttcc ccttcatagg
     3841 taacgactgt gtgtcaaaaa ggcctttttt aaacatccga tagagtgtac gaacagaaca
     3901 atcaattgga aactcgccac ggccgataat cacatcgggt gaccatcctt gagctacctt
     3961 ttcttgaaca tatgttgatt gttcatcaga taaaacggtt gaacgtcgtc cacaacgaga
     4021 tttattttgt ttgtattgac ggtaataatc tagcactttt tttccttgtt tgagaaacga
     4081 ataaacatta taaatggttt gtctagagcg atttaaactt tgtgcgacaa gagcgacagg
     4141 tttctcgata ttgaaataag actctatgat tactagttcg tctgttgtaa gatgggtgta
     4201 agtcatttgt actcactcct tattaattct ttggtcggaa ctattttgag tgtagcacaa
     4261 atgatctttt tagttgtcta gcttaatttt acaatcgacg ttttaataaa ttgtgatata
     4321 atgaagtata actttttagg gagtgtacta tgaattttca agataataga ccattatatt
     4381 tacaagtaga agcttctata cgtaacgata ttcgtgaaaa aaaatatctt ccaggagagc
     4441 aattacctac agaagaagaa ttatgtaaaa tttatggagt aagtaagatt accattcgaa
     4501 aagcttttaa acttttagca gaaagtggaa cagttgaacg attaaggggg aaagggacct
     4561 ttgtaactca aaaaaaagaa agtttaaaat tagctggagt tcatggattt agcgattctc
     4621 tttctacccg aggacataaa attagatata ctgttttaca
//
LOCUS       DAZKCY010000154         4511 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000154 DAZKCY010000000
VERSION     DAZKCY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.156"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..879)
                     /locus_tag="ACG8XC_002548"
     CDS             complement(<1..879)
                     /locus_tag="ACG8XC_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9192213.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVD"
     gene            complement(881..2320)
                     /locus_tag="ACG8XC_002549"
     CDS             complement(881..2320)
                     /locus_tag="ACG8XC_002549"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP9192214.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2505..4451
                     /locus_tag="ACG8XC_002550"
     CDS             2505..4451
                     /locus_tag="ACG8XC_002550"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFP9192215.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1428 a          777 c          752 g         1554 t
ORIGIN      
        1 atcaacaata agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa
       61 aaatttctca ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt
      121 aagtttaagt tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa
      181 aataccatct ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt
      241 agacagatta ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag
      301 tgcacgtaat tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg
      361 acaaccattt ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga
      421 aacaatggaa acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg
      481 ctcataatca gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc
      541 ttcaagcatt tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat
      601 cgttttatat ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa
      661 gatattacta atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc
      721 acgtgctaaa ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt
      781 tgttttttct gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac
      841 tccagctaat tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt
      901 attttccagt aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata
      961 cacgactagt aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta
     1021 caaaaatgtt agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt
     1081 cagtggcata ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat
     1141 ctacaatcaa gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct
     1201 gatttggagg aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa
     1261 attgttcaga actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac
     1321 catcttttat tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag
     1381 ttggataatc gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag
     1441 tattgaatcc ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag
     1501 cgcccacaag ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat
     1561 agatattccc ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag
     1621 gttttttgtc tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc
     1681 caaaatcaag gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa
     1741 gtttgataaa gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt
     1801 cataattaaa catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa
     1861 gaacattctc acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg
     1921 tatcacgttc ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct
     1981 ctatttcata tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa
     2041 gttctgctcc tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt
     2101 tcaacccatg agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa
     2161 aaaaagaaaa accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag
     2221 cacgccaagg tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat
     2281 cagaataagc acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat
     2341 ctttttacaa ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa
     2401 aataaactat atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt
     2461 tcagtgaacc gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag
     2521 tagcttccga cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg
     2581 ctacacgtct tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg
     2641 aaaatccaga tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg
     2701 caggtgatgt taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt
     2761 caacagatga tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat
     2821 taattaaatt attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt
     2881 tattaatggc attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg
     2941 aagaaatgta tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac
     3001 cattcatgtt cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc
     3061 aatttcttgg tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg
     3121 aatattggaa tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc
     3181 cagttttagc agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc
     3241 catcatctat tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga
     3301 ctttcacagt tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg
     3361 tttggctata taacactact ggatttattg gaatgggtat ttttggagga acatattcac
     3421 ttatagtcat gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag
     3481 cgtggacaaa tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg
     3541 ttgctcaagg tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa
     3601 gtcttgcttc ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg
     3661 gagttaactt aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta
     3721 cagcagtagc aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg
     3781 gatttctttc aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct
     3841 ttgtagttgc ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta
     3901 aagcagaaga aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc
     3961 caatatctac tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc
     4021 ccatttctgg taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag
     4081 ctatgggaaa agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg
     4141 gtactattca actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg
     4201 ctgaagttct aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac
     4261 aaaaagttac tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt
     4321 caatgattga gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg
     4381 attattcaga agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg
     4441 aaatcaaata aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat
     4501 atagattaat c
//
LOCUS       DAZKCY010000155         4505 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000155 DAZKCY010000000
VERSION     DAZKCY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.157"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1987
                     /locus_tag="ACG8XC_002551"
     CDS             47..1987
                     /locus_tag="ACG8XC_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFP9192216.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2074..3996
                     /locus_tag="ACG8XC_002552"
     CDS             2074..3996
                     /locus_tag="ACG8XC_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9192217.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(4276..>4505)
                     /locus_tag="ACG8XC_002553"
     CDS             complement(4276..>4505)
                     /locus_tag="ACG8XC_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFP9192218.1"
                     /translation="IDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAV
                     GSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         1492 a          768 c          931 g         1314 t
ORIGIN      
        1 aagaaaccgt ttttatatac tataaatgta gaaaggcggt aggaaaatga aaaaaattat
       61 tgcaatcaca tcctgtccag ttggtattgc ccatacatac atggcagcag aaaacttaga
      121 gaaagcgggg aaagcagttg gcgccgaggt aaaagtcgaa acacatggtt ccatcggtat
      181 tgaaaatgaa ttgactgcaa gagatatcga agaagcagct ggtgtaatca tcgcagcaga
      241 tactaaaata gataaatcac gattcggcgg taaacctttg ataaccgtag gtgtgcaaga
      301 aggaatccac catgcagatg agttggtgag agatatttta gcaggaaaag cgccagtcta
      361 taaaagtacg gaggcaatta tttcttccga aaataaaagt gaatcagaaa atttaggtaa
      421 aaaaatctat aagagtttga tgaatggcgt ttcgtacatg gtacctttcg tagttacagg
      481 agggttattg atcgcgattt ctttaacatt aggaggaaca gctacacctg aagggatcaa
      541 gataccagaa ggaacgattt gggcaacgat gaatagtatc ggaagtatcg ctatggggtt
      601 gatggttcca attctatctg catttattgc gcaaagtatt gcagatcgtc caggacttgt
      661 tcctggattt gttggtggga tgttagcggc aaacggagca ttatatggta gtgatgcgaa
      721 tgcgggattc ttaggcggga tcatcactgg atttttagca ggattcattg ctttaggaat
      781 caagaaagtg cgagtaccga aagctttaca atcgatcatg ccaattattg ttatcccaat
      841 tttaggatca ttggcagttg gttttgtgtt tatctatgtt attggagaac cagtagctct
      901 tctatttagt tcattgactc actttttagc agggatgcaa ggaggaagtg aaattgttct
      961 agctatgatc ttaggagcga tgattgcttt tgatatgggg gggccgttca ataaagtagc
     1021 ctttttgttc ggatctggct tgattgcaga aggcaattat tccattatgg ggccaattgc
     1081 tgtagctatc tgtatccctc cattagcatt ggggctggca tcattgattt taaaaaccaa
     1141 atttacgaaa acagaacgag aagctggtaa ggcgtcctta gcaatgggac tgtttgggat
     1201 tacagaaggc gctattccgt ttgcttcaac agatccacta cgggtgattc caagcattgt
     1261 aatcggggca atgacaggaa gtgtgatcgc gatgctttca ggagtgacag atcatgtagc
     1321 tcatggcggt ccgatcgtag cggttttagg agcagtggat cacgtagcca tgttttttgt
     1381 agcagtgatt gctggtattg ctgtaaccgt gctaatgtta agaatcctta aaccgacatt
     1441 acaacaaact gcggttgctg cagcaaatgg tgtgacagta gcagatctat cagagactga
     1501 aacaagtgat gtttatacga aaactgaaac ttttgattct atcacggata ttatgtcaga
     1561 tgaagcaatc gttttaggtg aaacagcaac aacaaaagac ggagtaatcg aagatttagt
     1621 ccaacgttta gaaaaaacag cggcgatcac agataaaacc agatttaaag aagtcatcta
     1681 tgatagagaa catgaaagta caacgggaat cggtatgggg attgctattc cacatggaaa
     1741 atcagcttat gttcttaaac caacagtggc atttgccaga agtcaatctg gggtagattg
     1801 gcattcttta gatggaaaac ctgcacatat gatctttatg attgctgtac ctgaaaacag
     1861 ccaaggtgat atgcacttga aaatactgca acgtctatca cgtaaattga tggatgatga
     1921 cttccgtcaa gcactgatgg aagcgccaga taaaacaaca gttcaccaat tattaagcga
     1981 aatgtaacat aagatgaaaa atcgaccgtg atgagacata atagatgtgt tatcatgatc
     2041 gattttttat ttagaaggga gtttctgcac attatgaatc agcgtcagca atctttgatc
     2101 tatcaattaa gtgagaacaa taattatata acaacaaaaa agctagcaga gcatttccat
     2161 gtgtcagaaa gaacgattca tagcgatttg aataccattg aaacttggtt gagagacaaa
     2221 catctgcctt taatgtttga aagaaaaaaa ggtacaggta tcttgttgaa tggtctgcct
     2281 cacgaaaggg aacaactgaa acgagcgctt aatgaagaga ctcaaacaga aatggataaa
     2341 aaacagcttc gtcagttatt gattttccat ctgctagtag caaaagacgg atttagtctt
     2401 gaagaacttt cagaaaaact atatgttgga aaacgtacga ttcgtaaaga attgacagag
     2461 ttgcaaagct tttttgagta tcaccattta cagctagtat ccaaaacaaa actaggaact
     2521 tttcttaaag gcgatgaaca agaaaaaaga cagttgcttg ttaagacatt acgaaatatg
     2581 cagaaagaag atccacaaag tccgtcgttg aaagaattct tcgctaaaga tacattaaaa
     2641 gttatccaac atacattaca agatgttttt tatgaaaacc atttagaaca gccgaatgga
     2701 ctggctagtg tggagattca catctacttt atgctcgagc gaatgaaaca gtaccagaaa
     2761 gtgaaattaa gtaaagacga aaacgaagtc gtggaacaca cccaagcaca aaagttaagt
     2821 agtcaaatat tagcaaaact agccaccatc tatccaattg aattttctcc tgatgaaatc
     2881 aattatctgg ctttgcgtat cgccaatctt tttacaaatt cacaagcagc cacacgtttt
     2941 caaaaggaat ctagcgcact tgctgatcat ttgatcgaac aagtggaaca gtttctaggc
     3001 tattcattaa aagaagatca gctactaaaa caaaatttaa ggtctcattt atcttcaact
     3061 tattttcgac tccattatgg tttgcagatt tctaatccat tgacgaaaaa tgtgtttagt
     3121 acgtacacac aacttttctt agttttacag ttaattttag aagattattt tgaaaaggaa
     3181 aatttttatg ttccacaaga ggagacagct tatttaacga tccatttcca agcagcgatc
     3241 gagcgacaca aacatcagaa aagtagaaga tatcaaacgg ttttagttag tgaatattca
     3301 aaagctatgg ctacttttct cgaagcacgg ctgaacagag aacttccaga attaacgatt
     3361 atggatctag ttgaatataa gccagaatta aagagacaag attttccctc tgttgatttt
     3421 atcttggcta cgttaccatt taaacatata agcattccag taatcgagat ctcaccaatg
     3481 atcacagaaa ctgatctagc ctatttgaca aaatatatgc tggagcatgt gcctatcaag
     3541 aagaaaaaaa catttgatct agcaagcttt actcatccat ttctgatctt tcctcaactt
     3601 gaacggacag acccagccga tattttaaac tttatgggaa acgtcttagt ggaacatcat
     3661 tatgtggagc cggaattcat cgattctgtc ttggaaagag acagtcactc cagtactaga
     3721 gtagcaccat ttgtaacaat ccctcacggt aatccgctgt atgtgaagca ttcgatgatt
     3781 tctattgcta caatgaaaga accagtccta tggcacgggg aacagatacg tatcgtactg
     3841 ctattagcaa taaagaaaaa tgatttgaaa catgcagagt tcaaaaaaat attttctgtt
     3901 attcactatc tagaaaagca gccagaacaa atggctcagt tgatgcaatc caatcatccg
     3961 ctagaaatct taacgttgtt atctagttat gaataagtag cgattaaaaa actgccttta
     4021 tatttataat aattctatct atttatttgg aagtagaaaa ctaaatacaa aaaaagagga
     4081 tgtggccaaa gttgtggcac atccttttat tttgagtttc ttataatttt ttatagttgc
     4141 ttacgatgtc ttgactactg gtaaacggtg ttcaagagac aatttgtcgg caataaggca
     4201 caattttgta aaatatggaa agctattttt ttaacattgt ttcttagtgc ttgaaaaatc
     4261 attttgaata cttctttata cgatcgatgt acgttgattt aagtctaaaa cttttctttt
     4321 gattccttct ctgacttttc tgaattccgt taatatttct tcttcgctcc cgacagcttg
     4381 tgcaggatct tttaggtccc agtgaaggtg ttcgtgattg tttggaacgg cagggcactt
     4441 gtctttggca tcaccacaaa gtgtaatgat caagtctgcc tgagtaaagt aatctctatc
     4501 aatca
//
LOCUS       DAZKCY010000156         4463 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000156 DAZKCY010000000
VERSION     DAZKCY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.158"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..432
                     /locus_tag="ACG8XC_002554"
     CDS             <1..432
                     /locus_tag="ACG8XC_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFP9192219.1"
                     /translation="YHHRAIYTNEDALVIKKEQGFTGTETKSLFLKDKKDNNYVYLTF
                     TTKQTDFKALGKIVGKRLSIVSSEHMEEQTGQKQGAVSPFGYEKQIPIIIDEELLLNE
                     KLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN"
     gene            469..1479
                     /locus_tag="ACG8XC_002555"
     CDS             469..1479
                     /locus_tag="ACG8XC_002555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFP9192220.1"
                     /translation="MISYPLLERNTTIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGINLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1594..2307)
                     /locus_tag="ACG8XC_002556"
     CDS             complement(1594..2307)
                     /locus_tag="ACG8XC_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192221.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSSDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPTHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            complement(2480..3087)
                     /locus_tag="ACG8XC_002557"
                     /pseudo
     CDS             complement(2480..3087)
                     /locus_tag="ACG8XC_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            3610..4285
                     /locus_tag="ACG8XC_002558"
                     /pseudo
     CDS             3610..4285
                     /locus_tag="ACG8XC_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            4278..4439
                     /locus_tag="ACG8XC_002559"
     CDS             4278..4439
                     /locus_tag="ACG8XC_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192222.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMKYVADEANRK
                     QRKLVGLDK"
BASE COUNT         1547 a          728 c          848 g         1340 t
ORIGIN      
        1 tatcatcatc gagccattta tacaaatgaa gatgctttgg taataaagaa agaacaagga
       61 tttacaggta cagagaccaa aagcttattc ttaaaagata aaaaagataa taactacgta
      121 tatctgactt ttactacgaa acaaacggat ttcaaagcat tggggaagat cgtcggtaaa
      181 aggctttcta ttgtttcatc agaacacatg gaagaacaaa cgggacaaaa gcagggcgct
      241 gtttctccgt ttggttatga aaaacagatt ccgattatta ttgatgaaga gctcttatta
      301 aatgaaaaac ttgtttttgc accaggtaga ccagatcaaa cgatggtcat ccaaacggtt
      361 gacttaaaca aaatcatcca gctacttgag atgccgatat ttttgatgcc ggcaaacgaa
      421 aaaaacaatt aaccaagtaa aatagtgcat acttggaggt gaagcaacat gataagttat
      481 ccattattag aaagaaatac aacgattggg gttactgcac catcctcagg cgtatcagag
      541 gaacaccatt cgttactcga atcagcttta aagagaatgg aagaaaaatc tttccaagta
      601 gttactggaa agacgctttg gacgcaagat aaggaaaaat caggtccagc aagaaaacga
      661 gcagatgaat tcaatcaaat gatgcaagat gaaaatatcc aaatgatctt tccgccttgg
      721 ggaggagaat tattgattga gattctcgat tttgtcgatt ttgatgctct acaaccaaaa
      781 tggatagtag gttattcaga tattagtacg ttgctacttg ctgtcacatt aaaaacaggg
      841 atagccactg cacatggaat caaccttatt gatttaagag gagagtactt ggatgaaacg
      901 acagcaatgt gggaaagtgt actgaaaaca agagaaggag aatcgattct acagcaacct
      961 tctgaaaagt atcaaaaaga gtggccccat gagcattcat catcttctgt tttccatctc
     1021 actgaaccaa caaattggca atcagtcagc ggagatgcag taaggatgaa gggacgatta
     1081 ttaggtggct gtattgatac gatcagtcat ttaatcggta ccccttatgg agatgtccaa
     1141 tcttttaaaa gaaagtataa tgatggaaag ccgatcttgt ggtttctaga aaattgtgag
     1201 ctatcgtcag ttgatttaag aagatcactt gttcagatga aactggcagg atggtttgat
     1261 ggcagctcgg ggatcatgtt cggtagaagt gcagcagagg atttgttaga ttataaaatg
     1321 aaggatgtat acgaagatct gtcaaaggaa ttaaaactac cgatcttgta tgatattgat
     1381 tgtggtcatg taccgccaca actgacattg atcaatggtg cctatgcaga agtagcggta
     1441 gaagatagaa aagcgactgt tttgcaaacg tttatttaaa aaattttttc ctgccttgga
     1501 gtttatcaaa gaccaacaaa tctaaataga aaagaaaatg aaagaagctg acagtatttt
     1561 gccagcttct ttttgtatga tctattagaa attttaatta tccataacta tcacattgat
     1621 cagctgtttc acatcatttg gattacctgt aggaatagct actacagatg cattatcaat
     1681 aatttcttga gttgtttctc taatgaacgc tggataagat gtttgatctg caggcatttg
     1741 ccaatgtgta ggaactggat aactagtgat tgtcccgtca tggttacttg aatacactaa
     1801 aactccctgg taactgtatt tcccagtgag cataaccaca tctttcggat agactgcact
     1861 ccctgcatca taaggggcga taggtgttcc tgcaggataa tgaataacat gtagttcgaa
     1921 atcactcgat gtatctctaa aagtattacc tctaatagct agccaaacac gtgcgtactc
     1981 aatttgtaga tcagaatatc ctgctaacgg atctattgac tgagaagact gtgaggaagt
     2041 tgtgtctatt gttgaagaag aaatatttgt ctcaactgta ctactagatg tagaagaagt
     2101 attgggctct gagactttac tgcttgttga agatgtattc gtttcagtag aagctgattt
     2161 ctgagtactt gatttttcag ctgaactggt ttgtctgatt gaacttttgt ctgtggtcgc
     2221 agatggatca ctattttctt tactaggaga cgtacatcct acaaggaata aagagaccaa
     2281 caataaaata aaacctaact ttttcatata aaatacctca ttattttttt aataccagta
     2341 tagcgtaatc ctgaattttt gaatccgtaa aaccttgtct atgaatataa atagagtata
     2401 tcttctttga taaatgtcag aaaaagtgtg acaaataacc atcagtaaaa ttacttgtca
     2461 tactattgag aaggagtatt taattgaaga gaatgattag gagaaaggat ctccgtactt
     2521 tttatagtaa tcatagaatc caagtttgta tagacgaatc gttttttctc ttatttcttc
     2581 tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat tctataggag ctgttccatt
     2641 cgcagtaatc aaattacgat ctttcacagc ttgttgttct ataaaatctt ctttattctg
     2701 ataattttcc gattctaacc aaggaaattg cgcatttccg gtgtgtttgt aaccttgcaa
     2761 taatccgctc tgagctaaat aatcaactgc cccacaaata gcagctactg gtacgtctgt
     2821 ttttaacaat ttatcgatta tttgatataa atgatgattt tttatgtccc atgaattgcc
     2881 accgatcata ataaaaaggt caactttttc aggtatttgt tcgattaagt aatcgatttt
     2941 tgtttttaat cctccaatgg aagtgacctc tgattggata gaagcagttt tgttttgcca
     3001 tccctctttt tggtttagtg tgctagtaat ataagcacct tcccaatcgg catattcctc
     3061 taatagaaac gtaatcgcag ttttcatttt caagctctcc tttactatct atgacaagga
     3121 caacagtttt aacatacaga cattcattaa atttggatag cgacagcgaa aacaacaatt
     3181 cttaagtttt agacagagta tacagacagt caacttaatt aaatacgaac taaataaaaa
     3241 tgacaggtaa tcatttttgt ttaccagcat tataacattt tgcatacatg gatgacaaaa
     3301 gaaccaattc ccacttgctt gagtacaatc ttaaaagaaa ctggaaggca aaaagtaatt
     3361 atagaaacga aaaataaatc atatcgggca ctcagacatg aaaacaaccc tagaaatata
     3421 taactagtcc aatgaaaaaa cagcgaatgc cgtagaaaac aaggattctg cgtacattca
     3481 tatctcattt tgcgagcata tctgctatta ctgtgactaa atgttaatat ggttaaaatg
     3541 accgttatat tttttatata aatatcgtat aattagtatg aatatagaag aaggaaagaa
     3601 ggtgcaatct tggaacctaa agacaagtat ttagattacg ctagggtcaa taaagataaa
     3661 tttattgaaa caatcatcaa gggaaaaatc cctgatgatg aaaaagatgc ggtattcatg
     3721 gctggaagcc caggagcagg taaaacagag gttgcactag gtttagctga aaattatgac
     3781 aaccatgttg tgattgatgc ggactatttt agagcacaat ttccagaata caatggaaag
     3841 aactcaagtg tttttcagaa agcttcttct tggttagttg aacaatcatt aaaatatgta
     3901 ctggaacatg gctattcttt catactagag gatacttttg ctatccttag tgcagagaaa
     3961 aatattattc gagcccttaa aaataacttt cgcgttacga ttttctatgt ttaccaagat
     4021 cccaaagttg cctgggattt cactcgaaaa cgtgaactag ctgaaggacg tcatgtgcca
     4081 aaagaaatgt ttatcaatgc gttttttaag tccagagaga atattgaaaa agtcaaaaat
     4141 agacatcctg aagtagtcct tcacataatg attaaggatt atcaaaacga catttctgaa
     4201 gtgcattatg cagcagataa tattcaatta gtactaccca atacaatata cttcaaaaga
     4261 gttggaggag gaattacatg actgagaaag aacaagtaca acaaatcgtg aagaagtata
     4321 ataaaagtat cgctgattta tcagaaaacg cctcagcgaa agaatttaaa acagtaatga
     4381 agtacgtagc agatgaagca aatcgtaagc agcgaaaact agttgggttg gataaataaa
     4441 ttttttaaac catatccttg taa
//
LOCUS       DAZKCY010000157         4446 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000157 DAZKCY010000000
VERSION     DAZKCY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.159"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACG8XC_002560"
     tRNA            2..74
                     /locus_tag="ACG8XC_002560"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACG8XC_002561"
     tRNA            78..150
                     /locus_tag="ACG8XC_002561"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACG8XC_002562"
     tRNA            155..225
                     /locus_tag="ACG8XC_002562"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACG8XC_002563"
     tRNA            238..311
                     /locus_tag="ACG8XC_002563"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACG8XC_002564"
     tRNA            323..394
                     /locus_tag="ACG8XC_002564"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     gene            871..1185
                     /locus_tag="ACG8XC_002565"
     CDS             871..1185
                     /locus_tag="ACG8XC_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9192223.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            1201..1587
                     /locus_tag="ACG8XC_002566"
     CDS             1201..1587
                     /locus_tag="ACG8XC_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9192224.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1616..3001
                     /locus_tag="ACG8XC_002567"
     CDS             1616..3001
                     /locus_tag="ACG8XC_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9192225.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            3004..3156
                     /locus_tag="ACG8XC_002568"
     CDS             3004..3156
                     /locus_tag="ACG8XC_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9192226.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            3179..4384
                     /gene="mobT"
                     /locus_tag="ACG8XC_002569"
     CDS             3179..4384
                     /gene="mobT"
                     /locus_tag="ACG8XC_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP9192227.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         1410 a          821 c          974 g         1241 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaat
      541 agcataaaaa tctagttatc cgcataaaaa ctggacttat cacactttat caaggtcaaa
      601 accactcaat ttactactaa tttactactt atgaatgagc tttgatacga cgatttatcc
      661 ttgaaaagtg aagatataaa gatacttcca ataaaatttg aatatttaat aggtagacac
      721 ttcaaaaaat gaggtgtcta tttttttacc cgattttgaa aggaagtgaa cttatgaaaa
      781 caaaaaatca agaatcaaaa ggtcgttccc cactctttaa gaccatcaaa cattcattca
      841 gccaataaaa aagaaaggat aggtaaaaat atggaactta aatttgtgat tcccaacatg
      901 gaaaaaacat tcggcaattt agaatttgct ggcgaggata aagtcgttca gcgaagaatc
      961 aacggacggc taactgtctt atcaagaagc tataatctct attctgatgt tcaaagagca
     1021 gatgatattg tggtggtgct tcctgctgaa gctggcgaaa aacatttcgg ctttgaggaa
     1081 cgtgtgaagt tagtcaatcc acgtattacc gcagagggct acaaaatcgg cactcgtggt
     1141 tttacaaatt accttttaca tgctgacgac atgataaaag aataaagaaa gagaggaaaa
     1201 atgatgagat tagcaaatgg cattgtatta gataaagaca cgacttttgg agaattgaaa
     1261 ttctctgctc tacgtcgtga agtgagaatc caaaatgaag acgggtcggt ttcagatgaa
     1321 atcaaggaac gtacctatga cttaaaatcc aaaggacaag gacgcatgat tcaagtaagt
     1381 attcctgcca gcgtgccttt gaaagagttt gattataacg cacgggtgga acttatcaat
     1441 cccattgcgg acaccgttgc tactgccacc tatcaaggag cagatgttga ctggtatatc
     1501 aaggcagacg atattgtgct gacaaaggat tctagttcat tcaaagctca accacaagca
     1561 aagaaagaac cgacacaaga caaatagtcg ctaggtagaa aggagacttt ttcgcatgaa
     1621 acagcgtggt aaaaggattc gcccatctgg taaagattta gtctttcatt ttacgatagc
     1681 gtcactcctg cctgttttcc tgctggttgt cggactgttt catgtgaaga caatccagca
     1741 gatcaactgg caggatttta acctatcaca agcagataag attgacattc cctatttaat
     1801 tatcagtttc agtgtcgcaa ttcttatctg cttgctggta gcgtttgtat tcaaacgggt
     1861 tcgctatgat acggttaaac aactttacca ccgtcaaaaa ctggcaaaga tgatacttga
     1921 aaacaagtgg tatgaatctg aacaggtcaa aacagagggt ttctttaaag atagtgctgg
     1981 tcgtacaaag gaaaagataa cctacttccc taaaatgtat tatcgactta aaaatggctt
     2041 gatacagata cgggtggaaa tcacgctggg aaaatatcaa gaccaactct tacacttgga
     2101 aaagaaatta gagagtggct tgtactgtga gctgacggat aaagagttaa aggattccta
     2161 tgtggaatat actttgctct atgacaccat agccagtcgt atttctattg atgaagtaga
     2221 agctaaagat ggtaaacttc gcttaatgaa aaacgtatgg tgggaatatg ataagctccc
     2281 tcatatgttg attgctggtg gtacaggtgg cggtaaaact tactttatac tgacactgat
     2341 tgaagccttg cttcatacag attcaaaact gtatattctt gacccgaaaa atgctgacct
     2401 tgcggactta ggttctgtga tggcaaatgt ctactataga aaagaagact tgctttcttg
     2461 cattgaaaca ttctatgaag aaatgatgaa acgtagtgag gaaatgaagc agatgaagaa
     2521 ctataagact ggcaaaaatt atgcttactt aggtctcccg gcacacttct taatctttga
     2581 tgaatacgtc gctttcatgg aaatgctggg aacaaaagaa aacaccgcag ttatgaataa
     2641 gctgaaacag attgtcatgt taggtcgtca agctggcttc tttctaatac tggcttgtca
     2701 acgtccagac gcaaaatatt taggcgacgg aatccgtgat cagtttaatt tcagagtggc
     2761 tttaggtcgt atgtctgaaa tgggctatgg catgatgttt ggcagtgacg tacaaaagga
     2821 tttcttctta aagcgaatca aaggtcgtgg ctatgttgat gtaggaacaa gtgtcatatc
     2881 agagttttat actccccttg taccaaaagg atatgatttc ttggaggaaa ttaaaaagtt
     2941 atccaacagc agacagtcca cgcaggcgac gtgcgaagcg gaagtcgcag gtgtggactg
     3001 atcttgctgg ctggtgtggc aatagccacg ccagcactta accccccgta tctaacaggg
     3061 gggtacaaat cgacaggaaa cagtcaaaaa aacattagaa aatcctttgg ttacaaggga
     3121 tttacaaaat ttcagcgtat gtcaaatggg ctttaaaagt tgacatacgc ctttttgatt
     3181 ggagggattt ttactgaatg aacaaacttg gttacagcat ttaaaagaaa aacgcttggc
     3241 ttatggacta tctcaaaacc gtttagctgt tgcgactggt attacaaggc agtatctaag
     3301 cgatattgaa acaggaaaag tcaagccatc agaggattta cagcagtccc tttgggaagc
     3361 tctggaacgc ttcaatcccg acgctcccct tgaaatgctg tttgattatg taaggattcg
     3421 ctttccgaca acagacgtac agcaggtggt cgaaaacatc ttacaactga aactgtccta
     3481 ttttcttcat gaggactatg gtttctattc ttattcagag cattatgctt taggcgacat
     3541 attcgtcctt tgctcccatg aactggacaa aggagttctg gtggaattga aaggtcgtgg
     3601 gtgcagacaa tttgaaagct atcttctggc acaacaaaga agctggtatg agttctttat
     3661 ggacgttttg gtggctggcg gtgtgatgaa acgccttgac cttgccatta acgataagac
     3721 agggattttg aatatccctg tactcactga aaagtgccaa caggaagaat gtatctccgt
     3781 cttccgcagt tttaaaagct atcgcagtgg cgaactggta cgcaaagagg aaaaggaatg
     3841 tatgggaaac accctctata tcggttcatt acaaagtgaa gtttatttct gtatctatga
     3901 aaaggactac gagcagtaca agaaaaatga tattcccatt gaagacgcag aagtaaaaaa
     3961 ccgttttgag attcgattga aaaatgagcg tgcctattat gcagtccgtg atttactcgt
     4021 ctatgacaat ccagagcata ccgcctttaa aattatcaat cggtatatcc gttttgtaga
     4081 taaagacgat tccaaacctc gttctgattg gaaactgaat gaagaatggg cttggtttat
     4141 tgggaacaat cgtgaacgat taaaactaac cacaaaacca gagccttact ccttccaaag
     4201 gacgctgaac tggctatctc atcaagttgc cccgacctta aaggttgcga ttaaacttga
     4261 tgaaatcaac cagacgcagg ttgtaaaaga cattctcgac catgcgaaac tgacagaccg
     4321 acacaagcag attttgaagc aacagtcagt aaaagaacag gacgtgataa caacaaaaaa
     4381 ataactcaaa tacaaattca ttgaatatag agaggagaac atttttatga attttggaca
     4441 aaacct
//
LOCUS       DAZKCY010000158         4416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000158 DAZKCY010000000
VERSION     DAZKCY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.160"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..913
                     /locus_tag="ACG8XC_002570"
     CDS             59..913
                     /locus_tag="ACG8XC_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9192228.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="ACG8XC_002571"
     CDS             1010..3259
                     /locus_tag="ACG8XC_002571"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9192229.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="ACG8XC_002572"
     CDS             3262..>4416
                     /locus_tag="ACG8XC_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9192230.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          625 c          806 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DAZKCY010000159         4402 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000159 DAZKCY010000000
VERSION     DAZKCY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.161"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            338..1261
                     /locus_tag="ACG8XC_002573"
     CDS             338..1261
                     /locus_tag="ACG8XC_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9192231.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            1277..1816
                     /locus_tag="ACG8XC_002574"
     CDS             1277..1816
                     /locus_tag="ACG8XC_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9192232.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            2497..2670
                     /locus_tag="ACG8XC_002575"
     CDS             2497..2670
                     /locus_tag="ACG8XC_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192233.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            3075..3509
                     /gene="bacA"
                     /locus_tag="ACG8XC_002576"
     CDS             3075..3509
                     /gene="bacA"
                     /locus_tag="ACG8XC_002576"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFP9192234.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
BASE COUNT         1647 a          655 c          739 g         1361 t
ORIGIN      
        1 cgaaccgtag ggagcaaggt ttggaattgg cagttttgat aaattcgcga acctgtttag
       61 agaaaaaagg agtgattttt taggcgggaa aattagcctc ccctcttttt atatctcttt
      121 ttatatctct ttttatatct cttttatgtc ggctgtgtcc ttactctccc aagcaattca
      181 gagttttttc aagtaggttt tctacgcttt tcaagtaggt tttctacgct tttcaagtag
      241 gttttctacg cttttcaagt aggttttccg acattattga gtatttttat aaagtatgtt
      301 acttttattt catactttat aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata
      361 tgaaaataga ttaaatcata ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt
      421 tttttcaatt gcttcacgtg tacgtgaaaa aggtgtagat gagattattt tttcatttca
      481 cgatttgaaa aaactaagcg gttatacaga acgtggggaa caatttataa aagatcttag
      541 tagcacgtat gacaaattat taagtattaa tgcttggaat gatgatggaa atactttaac
      601 taaatttgtt gctttcacaa aatatagcat tatacgagat aaacaacttg ttataatttc
      661 agttaatcca gaatttaaag gactttttaa tcaattaaag aactggacaa gatttaatct
      721 agagcaattt actaatttgg atagcacata ttcaaaaact atgtttcgat tattaaaaca
      781 atatcgaact caaggctgga ctgaattttc caaagaaaaa ttttttgatt tactagatat
      841 tcctaaaagt tattggaata gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa
      901 agaagaactc actcccttat ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg
      961 aggtaaacct gttatcggtt atcgttttac ttggaaagct gaaattaacc atgctgacga
     1021 tttttccaaa gggaaacaag aagatttacg tataaaatta tttaatattg atcacaatgg
     1081 agaattaaca cgagaagaaa aatggcgcgc taaagatcga atactaaatc ttcccttagg
     1141 cactcatgaa gctgatttta aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg
     1201 agaactttca aacaaactca aacaagattt actaggaaat ttacaaaact tattcgatta
     1261 agcttaagga gtatttatgg cagataaaac aatcaaagaa cttgcggaag aattaggagt
     1321 tagtaaaaca gcaattaaca aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa
     1381 aaacggaaac aagtttgtga ttaacgaaac cggtcaaaaa gctataaaat caatgttttt
     1441 agtcgataac gcaaacttaa atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca
     1501 taaaaacacc aaccaagttt ccgaattaaa actcctttct ttcctagaaa aacagttaaa
     1561 tcgaaaagat atacagctac aagaaaaaga taatcaaata aaacaaatgc aaaaattact
     1621 cgaccagcaa caacaactca ctctacaagc gaaccaacaa attgagaacc tacaagaaca
     1681 gctccattta acttatacag aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa
     1741 aaaagcagac aatacacaac cacttattga aaaaaagtgg tggcaaattt ggaaatctaa
     1801 tagaaccaat ctctaaaatt ttctagaagt tagttcggat agtagaggaa aaagttgtac
     1861 tacctgatgg ggagatcact caaattttta tgagaaagaa aaaatagttt acaactgtaa
     1921 acaatcaatg tttatagttg taaactatca aactatatgg tgacttattg cttaaaatag
     1981 gttgaaatct cactcaattt ttggtggaaa aacaaaataa attctaatcg attttgttga
     2041 aaaagtcata aaaaaacaaa tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt
     2101 cgtttatttt ttttgagaat tttgatcatc gtagggtcat tgtagaatca ttatgagatc
     2161 atcgtgggat catcgaataa agtatgggaa aggcttgcgg tgcttggtat taagggcatt
     2221 ctccgcctcc caggttcttg accattttct cttataagtg ggatttttac cacgttgaga
     2281 gaaaatggtc aagaacctgg gaggcggaga aaacatacca agcaccacaa gcaaaaaaag
     2341 ttagagtaac ttccaaaaaa gaaaaatttt ttagaacaac ttttgaaaat ttaattttga
     2401 tttatatccg ttcaatttaa acatttgata aactgaaggc tagtatttaa aatgtgtata
     2461 caagtggtag cgttaaaaaa agtggaggta taacctatgt taaaaaaaac tgtgttggta
     2521 ctgggttcta tttctctagg aatattaggt tttagcccca tgctattatc cgtaattgga
     2581 aaaaacaatt ccaacagaga acttaaattg aattcaaatt attacaacag tcctaacgaa
     2641 gaattgtttg taaaagaaac ggataaataa taaaaaagac tgctcttccg agcagccagt
     2701 taaaaaataa tgagtgttta ttcttgaaaa agcaacgcgc ttataaagcg ctaccaaaaa
     2761 tttatcatat tttatgaaaa ataacaatcc agtaaaaaat gactggtaaa aattcacaaa
     2821 gaaacggaaa gaagcaactg cagaaaatca agccttaaaa agagcttttt acctagacca
     2881 attaaaaatc acaaagtgag cccctgtatc ggttgataca agggctactt ggcatactgt
     2941 acattatgtc tactaacctt taaaataaca cgttttataa aataaaccct cttgacaata
     3001 tttaattttc tgattattct aaatatatac agaaagcgct ttcctaggat tttatatcta
     3061 aggaggaagt aacaatgaaa aataaaatta ttaaaattat tacttgtcta tttttgttcg
     3121 gtatgattgt tggagcaagt actccgttaa atctgtttcc ttttagtgct agaggtataa
     3181 aagtggaagc tgcaagttca cgatataatc ataatcatcg tggatttact tgctataaaa
     3241 tgaactatta tgttactaat aaaatgtgta gagatttgaa aaaaaattat aataagctaa
     3301 agactcctgc tcaaattgca tcttttattc ctattggtgg agttggaaca tgggttatta
     3361 caaatgcttt tagtagtgct gttgatgcaa tgaatgtctt tgtaagagct gcaaatcaag
     3421 gaaaaggtgt acaattaaca tataatgctc actttagcaa tacaacatca tatcaataca
     3481 atgattacgc tcgttatgta ataaaataat cagaaagtga aactatctca tgaataaaaa
     3541 gattttagct gtaattattt caataatagt tttaatcact atgttcttca tatttaggct
     3601 aattttcaat ataagcttac aaaaaagtat tctttatcta attcctatag catttatctt
     3661 agcaattttt agaagtattt acggaaataa aaactctaaa tagctaaaaa taaatcatac
     3721 taaattttac aaaaagtggc tagcattttc tagccacttt ttgtattgtg gagcgtgggt
     3781 tttgaggggg attcaagggg gagcttgcgc ccctttgaca agcggttatt tgcaccaaat
     3841 acggaggtat ttggctagca aatatagagc ttgccaaacc cttttagtga gttttgtaga
     3901 aagtaccttt aaaggtactt tcacattacg aacgatacgt ccgtaggacg tactgaaagg
     3961 gtgggactta tgagcaaatt aatgcaaaat ttggctagca aaaaagctaa aaaatatatg
     4021 ctaataatcg agcgaaataa acactttttc aagtgagcga atccgccatg acaaagctga
     4081 agcagtttat ttcagctttg tcatggcgtg tgccgacttt tttaaaagtc taggcacacg
     4141 agcaaatatc tttatcaagc tctaatttgc gttttaagac gtttgaaacg cctaagtata
     4201 taaatatata agaataggtt taagagtgct taaatcgaat tttagagggc atttagacaa
     4261 ttttagggtt gttagggctt gccctgaccg cctgtaaggc gttttaagaa caagataagg
     4321 gatttatact aggactgtct taaaatgtct taaacgaacg ctcagagctt ctcaaagggt
     4381 tccttatgaa cggcttttag ca
//
LOCUS       DAZKCY010000160         4387 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000160 DAZKCY010000000
VERSION     DAZKCY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.162"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(23..919)
                     /locus_tag="ACG8XC_002577"
     CDS             complement(23..919)
                     /locus_tag="ACG8XC_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9192235.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(912..1592)
                     /locus_tag="ACG8XC_002578"
     CDS             complement(912..1592)
                     /locus_tag="ACG8XC_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9192236.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1593..2375)
                     /locus_tag="ACG8XC_002579"
     CDS             complement(1593..2375)
                     /locus_tag="ACG8XC_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9192237.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2368..3285)
                     /locus_tag="ACG8XC_002580"
     CDS             complement(2368..3285)
                     /locus_tag="ACG8XC_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9192238.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3449..3703)
                     /locus_tag="ACG8XC_002581"
     CDS             complement(3449..3703)
                     /locus_tag="ACG8XC_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192239.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1290 a          949 c          765 g         1383 t
ORIGIN      
        1 agttctcact aatcttgtat aattaaaccc tccgcaactt gacggagatg gagaagatgc
       61 ccccgtctaa tctagcggtc atctctcctc ccattccttc caccaatttt tttgacaggt
      121 aaagcccgat tccgctagat tccgtatttg tgcggctggt attttcggta taaaaacgct
      181 ggaagacttt ttcaacagag gtttgcgttc ctggtttgat aggattggcc actgttaatc
      241 gaatgaatgg atcctctttt gaaaatgtca cctttgcttg tccatctgca taacgcaata
      301 aattctgtat gatattttgc agcactcgca gcaacatgag cggatctgct aatacagttg
      361 tatttatcga gtgttcagcg aacgctacct cgatattttt ctcagaaaat tgttcataaa
      421 aagagaaaaa tacttcttcc acctctttag aaaaagaaag cgtatggaga tccatttctt
      481 tttgcttcgt ctctattcga gcaagatcat aaaactgatc cgtcatactg agtaatcttg
      541 caacagattg tttgatgatc gttaaatact tctcccgttt ctccgtatcc tctacctgtt
      601 ccagtaattg tacataaccg ttaatagcag tcaaaggagt tctgagatca tgagaaaggc
      661 cgacgatcga tagtctgatg ttttcttcca tttcttgaaa tgcttgtcct ttttgttcat
      721 tttcatccaa ccatgtattc agtgcatcta ctaggtccat tagattcttt tctcgaaatt
      781 cgactgaaag acgaccacca tattcttgtc gttcagaaag agtggcgatt ttcttagtta
      841 atctcttgat ttccctttgc aggaagaaaa taaaacttcc cataaagata acaacgagaa
      901 gcaacagcca tttaatcaaa tctaaacccc actccccaca ccgtatgaat atacacagca
      961 tgtgcctttg ctagcttccc tcgaagattg gaaatatgca tattcactgt tttctcacta
     1021 tcaaaatatg gttcttgcca aactgtttca taaagatttg ctcgcgaaaa tacttttttc
     1081 ggatgttcta aaaaaagctt cagcagatcg aattctcttc ctgtcaaatg gacagccaca
     1141 ccattcacat ttacatccct cgactctttg tccagtaaga tttccttgta atagatgcgt
     1201 tgcgacagat cttttacttg gacagaagtc tgatgacgaa gttggatgcc gattcgtaac
     1261 aaaagttctt cgatatcaaa tggtttcgtc acatagtcat ccgctcctgc tgccaacaag
     1321 gaaactttat ctctttgatc gctttttgct gaaagaacga tgatcggaac attcgatgtc
     1381 tgtcggattt ctgctaacaa tgcttcacct gaaagccctg gaagcatgag atctagaagg
     1441 atcaaatcga caggatatgt gcttaacaat aattttcctt ccgtacctga ataagcatca
     1501 agaagtttgt atctctgttc aagaacctcc tccagtaagg aatgaacagc ttcatcgtct
     1561 tcgataatca aaatagtttc cagcaggacc acctacttaa tgtcagattt ttggaaaata
     1621 aacattccta gagctgatga taccacgatt gttactagac tgaccagaat cattttcggt
     1681 gcttgcgaat aagttgcttg ttgcgcaaag tccgtataca tgagccacgt ttccgtaaac
     1741 cactccgtta gaaattccaa gcctttgatt tttgcgaaaa caccgggaat gaaggccgtt
     1801 cccatgatat aaagaaaaga aagaagcaaa ctgacactgg tacttctagt gatgaatgtc
     1861 aaagccactg gaacagataa tgtccctaat gtcagcagaa gcaatacagg aaatactttt
     1921 agaacataat ttccatagtt tttgattccc tcaacttcac caaatccgaa agcaagtgtg
     1981 taagcaagca tggctagtcc cgtgatcaaa accacgccaa gtgttgtgat caaaagcgta
     2041 acgagaagtt tcgacacata ataagtcatt cgattccgtc ctaaagataa cgtattgcga
     2101 atcgtaccgt tttgaaattc ttccccaata taaaagcacg caaatgctgc gaaagccaga
     2161 agaatccatg cagccatttt ttctaccatt aaaatccctg ttgcaccgtt gatgactgta
     2221 tccgttgctt ggtttaattc attgccgtat tttgtatgca tataccattc gttccaagaa
     2281 taaaaaagcg gaagtacgac catcagtcct agtgagaccc acattttacg gctcttccat
     2341 acttttattt tttcagcagt taatagatta aacatgtgga ttgcctccag ttaattgtaa
     2401 gaaataatct tccagttttt gcccaactag gtgaatacca tccacaggaa cttttgcttc
     2461 taccaatgct tgattgatgg ctgctacttg gtcaattcct tcaaaaagct ggataacacg
     2521 gctagattgt acaaaataat ccctataacc aagagagtcg agaaccgtta ctgcttgttc
     2581 cgtcgccgaa gtgtcaattt ttataaattg acgagattct tgtgccaatt cttctttcga
     2641 taattctttg atcaagtgtc cttcatgcaa aataccatag tgagtggcga tctgtgacaa
     2701 ttcatctagc aagtggctag aaagcaagac ggtgatgccc cgttctttta ccaaacgttg
     2761 gatgatttct cgcatttctg ccatccctgc tggatccatc ccattcgtcg gttcatccaa
     2821 aatcaaaaat tctggattcg tgatcagtgc atttgcaata gctaaacgct ggcgcatacc
     2881 taaagagaaa ttctttgctt tctttttccc tgtattttcc aatcgcatca ttttcagcag
     2941 gtcttcgatt tctctatccg acacaccgcc gttcgctgct tggatacgta ggttatctct
     3001 tgcagttaat tctggataaa gagcaggggt ttcaatcgat tgacccatac gtcgtcttgc
     3061 tgcctgcaaa tccgtcgttc caaataattc gatcgaacct tcatcaatcg agatcaaacc
     3121 cataatagtt ctcataaagg tagacttacc cgctccattt tccccaatca atccatagat
     3181 cattccacgt ttgatttcga ttgatacttt atctaatgct ttggcagcac cgtatttttt
     3241 ggttatacca gttgctttca agaccgtttc atttccaaaa tccatactta tccttctttc
     3301 ttattgatga gtgaagtata gttgacgggt ataaagaaag cagccagaaa tgtataaaga
     3361 aagtgtaaat tttccaattg aatgtcaaaa aaaacaatcg gacaatacgt gtctttccta
     3421 ttatcaggca gtttgttata cgcttgtatc agtttcctga ttctttcttt tcgaccaaag
     3481 tcgtttctct gtattcctct tcactgaaaa tttttgcttc tttcagatca agcttgagta
     3541 attttaccag accggttata aggataacta tagcaagact ccctcccaga aaaattagac
     3601 tgtctcgcag acgtggttgc gttgtcttcc aatcaaagag cgcaccaaac agtgctaatc
     3661 caagtggact ggccaccatg gtaatcgttg ttatcgttga cataagacgt ccttgtatat
     3721 gcgttggaac ttctcgctga agccagacca tataaaaagc aaaagaaaac attaatcctt
     3781 aaaaacggac ttatgaaaaa taatacatgc cgtttgggat ttacaattag ctaccaaata
     3841 agaggttatg acaaaagact tttcacaatc ccttcttccg aataaacggt gttctcctgc
     3901 caactcctcg gtcacaagtc acaatattta tcaaacatga gaagcaattt ttgtaaattc
     3961 cttcttattt cttgaagctg accacttctg tcacaacctc agaagcggat aaataatatt
     4021 gtttgatgat actttatctt tttcccgttt gtttatagat cgaatgatat tatagaccaa
     4081 gaatcctacc tattaacata gctaaaaaca caaaaaaggc actttccaaa ttgtggaaag
     4141 tgccagaaaa gttaggatta aggtaactgc gctcagcatt gcttcgccca gtacgattct
     4201 cgtaactcgt gaactcgaac gataatcgca attaacgttt tgagaactgt gaagctttac
     4261 gggctttctt aagacctggt tttttcttcg tatcattgaa taagtttgaa aaaccttgat
     4321 ttataaaggt tttcccgtaa atatatttta ttatatttga ttaaatttga aatgtttgtg
     4381 ttctaaa
//
LOCUS       DAZKCY010000161         4378 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000161 DAZKCY010000000
VERSION     DAZKCY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.163"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..338)
                     /locus_tag="ACG8XC_002582"
     CDS             complement(<1..338)
                     /locus_tag="ACG8XC_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192240.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEF"
     gene            complement(546..1472)
                     /gene="glsA"
                     /locus_tag="ACG8XC_002583"
     CDS             complement(546..1472)
                     /gene="glsA"
                     /locus_tag="ACG8XC_002583"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP9192241.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1495..2922)
                     /locus_tag="ACG8XC_002584"
     CDS             complement(1495..2922)
                     /locus_tag="ACG8XC_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9192242.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2953..4167)
                     /locus_tag="ACG8XC_002585"
     CDS             complement(2953..4167)
                     /locus_tag="ACG8XC_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFP9192243.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1375 a          928 c          775 g         1300 t
ORIGIN      
        1 ataaattcaa gatcagccag ttctgtactc gtgaatatac cattaaaata ataacttaaa
       61 aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag agcaatacca
      121 tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat acaatattct
      181 agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac ccttagagaa
      241 atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca cagatacact
      301 tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg cctccagttt
      361 ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga
      421 aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa
      481 tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag
      541 aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata
      601 ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga
      661 tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg
      721 acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt
      781 gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc
      841 agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa
      901 taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa
      961 gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat
     1021 gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca
     1081 tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga
     1141 ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg
     1201 gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat
     1261 aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc
     1321 tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact
     1381 tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt
     1441 ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt
     1501 ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat
     1561 aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc
     1621 aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac
     1681 gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt
     1741 aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac
     1801 agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac
     1861 ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg
     1921 tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc
     1981 ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat
     2041 cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa
     2101 cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt
     2161 caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg
     2221 ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat
     2281 atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat
     2341 caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc
     2401 taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat
     2461 caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa
     2521 taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga
     2581 taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg
     2641 gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac
     2701 accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat
     2761 aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa
     2821 tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa
     2881 cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta
     2941 tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg
     3001 ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt
     3061 cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta
     3121 ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt
     3181 acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt
     3241 ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat
     3301 ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga
     3361 tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca
     3421 agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc
     3481 atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt
     3541 accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct
     3601 ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc
     3661 tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg
     3721 gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa
     3781 attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag
     3841 ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct
     3901 tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg
     3961 tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag
     4021 aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa
     4081 agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc
     4141 tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata
     4201 aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc atttataaaa
     4261 gacagacaat taataaaagc ttgatgtgtt gtgctctttt tcgtttttca cacaaataaa
     4321 gacaaaaaat aaacggtctt ctttttatag aaaaccgttt attttcgtct ttttttat
//
LOCUS       DAZKCY010000162         4283 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000162 DAZKCY010000000
VERSION     DAZKCY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.164"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..1353
                     /locus_tag="ACG8XC_002586"
     CDS             52..1353
                     /locus_tag="ACG8XC_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9192244.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1380..1640
                     /locus_tag="ACG8XC_002587"
     CDS             1380..1640
                     /locus_tag="ACG8XC_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9192245.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVTHLNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2639..3874)
                     /locus_tag="ACG8XC_002588"
     CDS             complement(2639..3874)
                     /locus_tag="ACG8XC_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9192246.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1243 a          686 c          884 g         1470 t
ORIGIN      
        1 gcgttgtcat gcaatgaatg gttcatattt tattgaaaag gagtggtttt agtggaaaag
       61 ctgatgaaat gggtcgaaga gaaacttgtc ccaccaatgg caaaaattgg tacacagcgt
      121 catttattag caattcgtaa tggggttgtt tcaacattgt cattgatttt gattgggaca
      181 ttttttatgg tctttatcaa tttaccgttt cctggatgga atgaatttat tgccccttat
      241 tcggcaacga ttgttttacc ctttaggatg acgatgggat tgatggctat ttatgcaact
      301 tttgttatgg gttcggatct agctaaatca tatggattag atagtgtgac aggtggtata
      361 ttatctttag gaacgttctt tatgttacaa gttcctgtaa acgttttaac tcctgaagag
      421 gcacctcttg gctgggtctt acctatgagt tctttgggag catctgggat gtttgcgggg
      481 atcttatcta tgatttttgc agtggaagta tatcgtttct ttaaaaatcg aaatattact
      541 atcaaaatgc cagaacaagt gccacctgct gttgcccgta gttttgaggc attgattcca
      601 ggtgcagtca ttttaacaat tacttggctg attcgaagcg tcgcgggctt tgatgtaaac
      661 gctgccttat tatcgttatt cgagccttta accaatattt tagggaataa tttgcttggt
      721 gttttactac caatgttttt gatccatttg ttgtggtcat ttggtatcca tggcatgagt
      781 attgtagggg ctgtcgttcg tccaatgtgg ctgatcatgt tggatgagaa tgcaaaggca
      841 ttggcagatg gtacagctgc cacccaatta ccctatatcg ctcctgaaca gttttatcaa
      901 tggacggtaa caattggtgg tgcaggagca acgatcgtgg tatctgtttt gtttatattc
      961 ttctgtaaat caaaattctt gaaagaggtt ggtcgttttt ctattattcc tggtatcttc
     1021 aatatcaatg agcccataat ttttggagct cctatgatgt tgaatcctta catgttcatc
     1081 ccgttcaact tagttccttt agttttgact attatcagtt attttgcagt caaattagaa
     1141 atggtcaata gatttactgt tttgccaaca tggacattac cagctccaat cggcggttat
     1201 ctttcagcag gaaatgactg gcgtgttgtt atcttgatca tgatcaacac attgatagca
     1261 tttattatct attatccatt tgtaaaagct tatgataaaa agatgctgtc agatgaacaa
     1321 agtcgtggag aaaacgaagt ccagactgtc taagtttggt ttttagaagg tgggaaacaa
     1381 tgtattggct gattttattt tttgttttta tctttttatt aacggctagt caccttatat
     1441 tgaatatgct agctacatat catattcaaa tcaatcgatg gatttgggca ctggcatcat
     1501 ttttgatcgt gattcttcca aagatcattg taacgcattt gaatgttttg ttttcatggg
     1561 ggacatatgt actttgtgga atttttgcta tcaattttat gatcgagcag catagatggt
     1621 ttgtcacatc gaagctttaa atgttctaaa agtggttgta acagaagtgg ttagcttcaa
     1681 gaaataagaa gaaatttacg aaaattgctt cttaaatttt tgtgaaatgt tggcttattt
     1741 ttcgtttggt tgaacttatt accgcagatg caagcttttg accaccgttt attcggaata
     1801 tgggactgtg acaaggcttt tgtcacagcc cttttttaca taaagattat tattgtttta
     1861 actgttttgg cagaaagaag gaggaaaaaa tggaaatttt atcagcaatg gatttagaat
     1921 tagtagatct actaccaaag tgatcaagtc aaatatagat ttgggagctt acaaccatat
     1981 agtaggggct gcagtatggt gtatgaatgg taatatgtat gatgtgagtg tttattcaaa
     2041 tcatgaaacg tgtatagaaa taattgtgct gggaatagca atcagtcatg gtgaacgtga
     2101 cttttagtgt atcgttgctg acgacataga acattctgac atgatctact caatatgtgg
     2161 ggattgctgg cagtttatat aggagtatgc atcggattgc gagatcattg tctcaacaga
     2221 agagggaggt tgattatcaa agataaagtg gtaaatccta catttgattt agtaattgtt
     2281 gaggggacta gcggcacaaa tctctacaaa gcgatgcttc tagatctata tgacacattt
     2341 taagaaaatg tatttgccta attattatgg tctttctttt gaagaaacgt tgagatgatc
     2401 caaccagaga aaggtgtcaa agaatttgac taagaataaa tggcagacgc tacctatgct
     2461 catttggata gcgtcagatc ttttatttta aggatgattt tgtgtttttt agacaccgta
     2521 atttaagtaa ctctaacttt tcaatccact atttactttt tttgtatttt cactgtctgg
     2581 gtctcgaata gcgaaattac tgatgagtct ctattaaaaa agctttttct acttgaaact
     2641 atattgggtg gttgctcagg aaggtttcga tttcttgaat tgcttgttgt ggaccattta
     2701 ttggaaaaac ggcttgcagc gtgcgtgcct gctgacgaat cttgggtgaa gaaacttcgc
     2761 gcagagcaat ctgcagtttt ctttcagtca ctggttttgt gagggcgatt gcgtttccgt
     2821 attttacaca atagtcaata ttacagcttt gctcaaattg catccccaca ccgataaacg
     2881 gtttgccgga ttcacaggca gtttggacgg tcccttcccc gccgtggata aaagaaaaat
     2941 caatgaattc atgcaaaaga tgactagaca gatattctgt tattaaaaga tcttctgagt
     3001 cagattgaat gtcatcaagc agatagtcaa taggtgcaat aaccgctaga tccggttgtt
     3061 tttgtaagaa gaacaaaatt ttgcgtaaca tacgcggatt gcctgaacta cccattgcga
     3121 aatagataat acgttttctt tcttgttgct ttcgctgtat aaaagttagc acttcttcag
     3181 gcaaggaagt actgagctta gcaaaaattg gaccaacgat tcgataattt tccggcagct
     3241 gttcttcttc caaaaatagt ttaggcgttg tgatgagatt tagatcagca tccatcattt
     3301 gtaaacttgt ttttggtaaa ggaagcattt ctttttgtgc agcgtgtttg aaggcattgg
     3361 ggatccatcg ggtatttact atacgctttt ttgtccagtg ccaaagggga ttgcttaaag
     3421 ggccgaagaa acgtaactcg tggggcatca gcgcatgctc tgattgaaaa aaaggttcac
     3481 tcaaagcata gggtttaaca tatactaaag gaatcttttt attgcgggcc gataaaaaaa
     3541 ttgtcacatt cgaacctgtc acaatcagtc gaggctcaaa cttagcaatc agactctgtt
     3601 cactagtgat acgagaggca accatttctt tggtaaaagg gtttttaagt gtacgcaact
     3661 ggtcaaattt tatgatttgt tcttcttgtt ttttcgtaag gatcggcgct agtaaatgat
     3721 actcaaagcc actttctgtg atcaaaggag caaaagtttt agaaaagcca aagaatacac
     3781 agtaataacg gtctttcata gctttggcaa cctcaatcat tcgtgttgtc tcagctaaat
     3841 tgaatactgc aggagcaaaa agaattgtct tcatgaaatc ctccttattg gaatgagtct
     3901 atcccttcgt ttcttactag tatactaaaa aacacagcgt tcgagtagcc tatgtatatt
     3961 ttgaatatta aactgtctct cgctgttttc acaacttcta aatttaaatt ttgtaatcaa
     4021 gttgaaactc caattttcaa aaaaacacgc aacaattttt tgatcgattt atatgttgtg
     4081 ccagataaat acgagaagtt gtgtcattaa aaataaacat gagaacataa aaaaataaaa
     4141 taattatatt aattgatagg tgtgctgtcg tcattataca cacgtaagaa agaaatagtt
     4201 atgtttaaaa atattcttag actttctcgc catgacaatt ttcactatgt gttataactg
     4261 taaacgataa ttattcagat aac
//
LOCUS       DAZKCY010000163         4260 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000163 DAZKCY010000000
VERSION     DAZKCY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.165"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..1474
                     /locus_tag="ACG8XC_002589"
     CDS             200..1474
                     /locus_tag="ACG8XC_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFP9192247.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(1857..2585)
                     /locus_tag="ACG8XC_002590"
     CDS             complement(1857..2585)
                     /locus_tag="ACG8XC_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9192248.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(2563..4137)
                     /locus_tag="ACG8XC_002591"
     CDS             complement(2563..4137)
                     /locus_tag="ACG8XC_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9192249.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
BASE COUNT         1336 a          732 c          766 g         1426 t
ORIGIN      
        1 gctctttgtc aaataggact gatagagtga tataagaaca tctaaggtag aattgatttt
       61 gcttctataa tattttgtgt tggtggaaaa tcgaaggcga ttttctacta acactttttc
      121 ttcgtttcaa ctcaaaaaga gactcacttt tggtaaaatt tagtcgacca aaacgtcatc
      181 ctaccaaagg agtctctcaa tggataatca tactagaaaa ttactcggat taacagataa
      241 acatttattt tttgatgaag aatggctgat tgaaaaagaa tataaaggcg ttcaagcgca
      301 gtttattaaa ggaaaattgg atgactctcc ttcctattgt gaacactgtc gatcggttcg
      361 tattatccgc aacggttcct atacgacacg cactcagata ttgaaagtca aagaaaagtt
      421 aactatttta gaattaaaac gtacccgttt tctctgccat gattgtggaa ggaccttttc
      481 tgcgaaaacg gacttagttg atgaacatca tcaactaaca aaggaactga agcaggcaat
      541 tctcatggaa ctgtacgaaa atcaatcacg taaactaatc gctaagaagt acttcgtctc
      601 ggatgggacg gtgacacgga tcttgcgtga agcaacgaag cattaccagt cacgtatgaa
      661 cttccttccg acggtgttgt gtatggatga gttcaaatca atgaagtctg tttctggttc
      721 catgagtttt atttgtgttg atgggacaac ccatcagcta tttacgatcc ttgaagatcg
      781 cagactttac aaactaactc aatacttcct gcactttcct agaaaagccc gtttaaaggt
      841 caagtaccta gtgatggaca tgaatgccag ctattgtcag ttgcttaaaa cagtctttcc
      901 aaatgcggag atcgtaactg accgctttca tattgtccaa cacatcaatc gcagcttcaa
      961 tcaattacgt gtccagatca tgaatcgttt tcgaacctca cactctgaaa atcagaagaa
     1021 gtaccgtcgg ctcaagcgat attggaaact cttactcaaa gattccacga catgggagcc
     1081 actcaaacgg cattatcatc gactgtttaa acgaccaatt agtcaaacag aaatcgttga
     1141 tgagctgtta agctacaacg aagaattgcg aactgcgtat catttttgtc aattattacg
     1201 ctattatttc gttaaacgag gtacggaagg ctttcaagaa acccttaaaa caatttcatc
     1261 tacgttacct cagtcgttta aaaagaaatt cacgatcttc cattgctacc aatcgggtat
     1321 ttcaaatgcc tttaaggtgt cccactcaaa cggcgtcact gaggggctga ataacaagat
     1381 caagctgatt aaacgaattg cgtttggtta tcgcaacttt tataactttc gggcacggat
     1441 ctatttacaa caaggtctga tctttgaaaa ctaaaaaaat atgcggcgca cagaacgcgc
     1501 cgcatatcgg actaagtttt attcaaattt gtcttcacca acacgatttg acgaagagcc
     1561 tatttaaata actacatcca tttattctat cttctaaaat agcctttttc atttcagtta
     1621 aatagttcag cgccattttt gcactcgact tgcatagtag ttttttccat attcctcaac
     1681 ctcagcgggg gttgaagaca taaggaaaat cctatgcgtt atgctcaact ggatctgatt
     1741 aaatattcta atgtattttt ctctaccatc attaaatggt taatagccaa aaatcaccac
     1801 ttttcaatgg ctccttgttg tataagtata actagaagat gaacacgtct gagaaattat
     1861 agaagctttt tacggtaatt actggggctg tgtccagtag tttttttgaa gagaacagaa
     1921 aaatagctca cgtgatctat tcctactttt tgactaattt catcaataga taagaatgtg
     1981 tctttcagta gagcacttgc tcttttgatt cgaaagtcta gcaagtaatc atgaaaggaa
     2041 taacctgttt cttttttgaa ggcccgtcct aaataagcgg gatggtaatg gaattgtgta
     2101 gctacatctt tgagcgtaag cttttcacta taattgtttt gaacgtaatc aatcacttgt
     2161 tgaattgact cattaaaaga atcatcagtt agagagtttt gattagctgc tgatttacgc
     2221 attttttctt cttgtactaa cttaactaaa taaggaaaat caatgggttt cgttatgtaa
     2281 cttttaatag ataaatcaat cccctttttt gcatactgaa aattttggtg ggcactaatg
     2341 acaatactag taaagtagcg attggatgtt tgttgcaaag attcgattaa ttcgagtcca
     2401 gaaattttcc ccatcgtaat atctgtgata agtaaatcaa agcgctggtt ttgcaggttt
     2461 ttaaatgctt cgtctccgtc tgtgtagcag tgatcgataa tgatgtcggg gactttcttc
     2521 aaaagataat gccttatgcc ttctacaatt gtacaatcat cgtcaacaat tgcaattctc
     2581 aacatgcata ttcccccctt tttgtatttt gatagtaatc gttgttccga cgtgtagttg
     2641 gctttctata acaaaacgtg tatctccagg atagagatgc tttatacgtc gaaaagaatt
     2701 tgagatgccg atattctcac tataaggaat atcatcatta agtttttgtt gaatatgctc
     2761 cagcttgtct tggtccattc ctttcccatt ttcaacgatt tttaacacat aatggttggt
     2821 tgtttcagtt gcatagacag tcaaacgatt ttcctttttt gatgaatcga agccgtgttt
     2881 aatataattt tcaacaaatg gctgtaaaga aagttttggt aacattaatt gatcaatatg
     2941 atcaatcagc tgatagtcaa agttatgtcg ataacgacgt tgatgtagtt gcaaggagag
     3001 gtcgatatta attaattcct ctttcaaatc aattatgtcg ggctgatatg tattattgcg
     3061 atacaacttg ctcaataaat atatactgtc gctgatttct tcgtcatcat tcaatatggc
     3121 agtcattcga ataaattcca aattattaaa tagaaaatga ggctcgattt gatattgaag
     3181 tgctgcatac tttgcttctt gcttcaacca attattttca taaagtgttt tttgatatgt
     3241 agcattatct aaataaaact gattgatatg catagcgatt tctttcaaat caccgacttt
     3301 gttttcgata ttgattgact tgtagtctcc tgtagatgaa tgatttaagg aagtaatgat
     3361 ttcctgatac tgctggatat agttacgaaa acttctattt aataattgat gaatagcaag
     3421 aataaacaaa ctcattaata agaaaatcat gattgccaga cctaataagg tttgtgacga
     3481 ataatttgga ttttcatata cttgtaatgt gtggttaccg atagctatct gttgcgtttt
     3541 tttccattta gaaggcgaga tgtcttcaga taaattgacg gaatcaatac cattagacaa
     3601 agaataattg taatgtgttg gaaagagact cttttttagc gcgttgatcg aaacgtctag
     3661 aatcaatgta gcatcgggca tgtcctcact aatgaaactt aatgaacgaa taaaagaaat
     3721 tttttcgttt tttgtaggtg cgtaggcact taagtatttt tcgtaatcta atgagataga
     3781 tatattttta acttcatcat cttgcacaag tttttttaag ttatctacta aataggtagg
     3841 ggagcgatcc tttgacaaaa tatcttccat gtattcagca ggtgatgaga ggtagtagtc
     3901 attaaggttt tcttggaaat cataattttg atagatctct gtcacgatac cggagagaat
     3961 acgattatat gtttccatat agtgggtgat ttgctgaatg ttttgcaggt tttgattttt
     4021 ttctttttca atcacattga tataggaaaa gaacaatgtg aaacaggcaa gtaaagtaat
     4081 aaaaaaaatc aatatgtatg taaaacgcat aataactttt gataaatcgt ttttcattca
     4141 aagtcctcat tttgataatc tctaatttta tgaatcatta tagcacatat tacaagtgat
     4201 gtaaacgctt tgcaaacagg gctacttatg attgtgaata cagtttccgt ttaaaaacta
//
LOCUS       DAZKCY010000164         4233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000164 DAZKCY010000000
VERSION     DAZKCY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.166"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..633
                     /locus_tag="ACG8XC_002592"
     CDS             64..633
                     /locus_tag="ACG8XC_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFP9192250.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            743..2497
                     /locus_tag="ACG8XC_002593"
     CDS             743..2497
                     /locus_tag="ACG8XC_002593"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9192251.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            2457..4223
                     /locus_tag="ACG8XC_002594"
     CDS             2457..4223
                     /locus_tag="ACG8XC_002594"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9192252.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
BASE COUNT         1408 a          592 c          808 g         1425 t
ORIGIN      
        1 agattcaact ttgatagaac agatttttaa tgacataacg aaatagaatt gaggagaata
       61 tatatgaaaa caaaagatta tatttttatt ggtttagcta cagttatttc cctagtaatt
      121 tacttttttt caataatggt tagtagtatt ggtggggcgt ttggtcacag catatcccca
      181 gggatttttg gattattatc tggaatcata tttgtttata ttagctataa ctaccctaga
      241 aaaggaattt ttactattta cacaattgtt ttattgttat tttttacgtt aatgggcggg
      301 gcgtacttac cttggtttat cagttcgatt agtatggcga tcttagctga tattattcta
      361 tctgtatttg gatatgatcg agcaattcct caagttgtgt cttgggttct aatgcaacta
      421 ggcagtgctg ccggacagtg gattcccata tggttcttta cagatcgttt tcgtcaagac
      481 tggatagata gaggacaatc tgctgcaaca atggatgcaa tgatccacta cgcggtgggt
      541 atatggggaa ttatttcagt gttagttgta gcaagtttat ctatgattgg tgtattgatt
      601 ggaagaaaag tcttaaaaaa atacaaaaaa tagaacgtag tttaccttct atctgtttgt
      661 acatctctaa tttacgctat tacggaaagc attaacagtt gtaaacgaat aagagaagaa
      721 attgaatgcg ggggaggaaa aaatgaaagt atacaaaaaa ttatttagtt atgtccctaa
      781 aatgaaatac tacggatggt tagccacttt attttctgat ttttctgtcg tattaacagt
      841 ctatggatat tacagtattt acaaatttct tcagtctcta attattgtag gagatgaata
      901 tttagctaag agttatgcgg ttcaaactgt tgcttggtta actttaggag ctttatttta
      961 tattttttct ggcttatttt ctcatatcct tgggtttcgc ttagagacta atttgagaaa
     1021 aaagggaatt agcggtttaa cagaagcaag ttttcgtttt tttgatttaa attcttcggg
     1081 atatattcga aagacaattg atgacaacgc ggctaaaaca catatggcaa ttgctcactt
     1141 gattccagat agtgctcaag ccatagtaac acctgtttta tcagttgcct taggcttttt
     1201 cattaacttt aaaattggat tggttctaat cgccatgtta gtaattggtg tattcttgtt
     1261 aaagaaaatg acagagaata caaattttat tcaaaaatat caagaatcgt tagatgtgtt
     1321 aagctctgaa acggtagaat atatccgcgg aattcaagtt ataaagatat ttggtgctaa
     1381 agtaacatca tttaaagcct tgaataaagc aattaacgat tatgctaagt ttgcatataa
     1441 gtattctttg agtgggaaaa caccttttgt tctttttcaa tggctatttt ttggcatagt
     1501 ttgcattttg atagttccta taaccttttt tataacttct cttaccactc ccaagatctt
     1561 agctgttgac ttgttgatgt tgtttttttt aagtggaatt atttttgttt ctttcatgag
     1621 aataatgtat gtaagtatgc acttgtttga agcaagatat gcagtcgaaa cactagactc
     1681 cctttattta gaaatgaagc aagaccaact aaaatatggg aacgagacac agattaaaaa
     1741 ttgtgacatt gcatttgaaa atgtcagctt ttcatacaaa gaagagtgtg ttttagaaaa
     1801 tttcaactta gttttagaac aaaaaagaat ttatgcatta gtaggaaaat caggaagcgg
     1861 aaagagtacg atagctaaac taatttcagg tttctacaat gtggataagg gacagattaa
     1921 aattggtggt aagcctttaa atcactattc aaaagacaca attattaaca caattgcttt
     1981 tgtctttcaa gatacgaaac tattcaatca gagtatttac gataacgttt ctatagcaaa
     2041 tcctcaagcg ccagcaagta aagtatttga cgcgctaaga ttagccggtt gcgaatcgat
     2101 tttagaaaaa tttcccgaaa gagagcatac cttaattggt agtaaaggag tttatctgtc
     2161 aggaggagaa aaacaacgta tagctattgc cagagcaatt ttaaaagatg caaagattgt
     2221 tattatggat gaagccagtg catctattga tccagataat gagtatgaac tacaaaaggc
     2281 ttttcaatat ttgatgaaag aaaaaacagt tattatgata gctcatcgat tatccagcat
     2341 taaatctgta gatgaaattc ttgtcttgtc agaaggaaaa atagtagaac gtggaaatca
     2401 taatgaatta atggctaaac atcgagtgta taaacaattg gtcactaatt atgagaatgc
     2461 aaatgattgg aggcttgaca atgaaaaatt tttataaaaa aaaatttgct ttaactgatc
     2521 agggcgcaga agctttaact aaagcttcta tttctagttt ttttgtatac tgcattaata
     2581 tggtaccagc attcattata atgatgctga tagacgaatt agttttagaa aatgcaaagc
     2641 cccgttggct ttattttgca gtatcgtttg ttactttgct tttcatgtat tggttgttag
     2701 atagggagta tgaaaactta tataatagta cttataaaga aagtgcgcat ttaagagtgc
     2761 aaattgcaga cgatttgtca aatttaccat tatcctattt ttcaaaacat aatttatcag
     2821 atttatctca aactatcatg tctgacgttg aaggtattga gcatgcgatg agtcatgcaa
     2881 tacctaaagc cggcggtatg gctctgtttt tcccttttat ttcagtgatg cttttggttg
     2941 gtaatgtcaa aatgggatta gctgttattt tgccaacgtt atttagtttt gtcttaatct
     3001 tgttatcaaa gaaatcccaa acgaaagcca atactaaata ttacgatact ttgagagaaa
     3061 actcggaaga atttcaagaa actattgaat tgcagcaaga gattaatagc tttaatctat
     3121 ctaaaaaagt tcaagacaga cttttcaaaa aaatggaaga gagtgaaagg attcatttaa
     3181 aggtagaatt aagtactttt tcagtcatgg ccttatcctc tattttctca tatgttagtt
     3241 tagcagtagt cattctagta ggtgttcact tactgttaac gggtgaagtg actatactct
     3301 acgtcgttgg ttacttacta gccgcaataa aaataaagga ttccttcgat tctatgaaag
     3361 aagcagtgct tgaaatattt tatttagctc ctaaaataca gcgaattaga gctatgaagg
     3421 aaaccagtat ccaagaggga tcagatagtc ctctgaaatc ttttgacatt gagttgcgag
     3481 atgtctcttt ctcatatgat aacaataccc caattttaga tcatatctct tttacggcta
     3541 aacaaggaga agtgactgcg ttagtaggtg ctagtggttc cgggaaaact agtatcttga
     3601 aattagtgtc tagactgtat gattatgatg aaggctgtat tttaattgat gggtatgata
     3661 ttaaacgtgt atctccagct tctttgttta gcaaaatcgc tattgttttt caagaggtaa
     3721 ctttgtttaa tacaagtatt ttggaaaata tacgtattgg aaatagtcaa gcgtcagatg
     3781 aggaagtgaa aaaagcagct cgtttagcta actgcgaaga ctttattgaa aaactacctg
     3841 acggttatca tacattggtc ggagaaaacg gaagttcact ttctggagga gagcgacaac
     3901 gcctatccat agcacgggct tttttgaaaa atgcgccaat tttaatttta gatgaaatta
     3961 ctgccagttt agatgcggaa aatgaaaaac gtattcagga aagtttaaat cgtttaatac
     4021 aagataagac agtactaatt atatcgcatc gcttgaagtc tattgaaaag gtaaataaaa
     4081 ttgtagtgat ggatcaagga aaactagttg atcaaggtac tcataatgag ttgtatcgaa
     4141 ggtcagaaat atacaaaaat ctcataaaga aaactaaact atctgagaaa ttcgtctatg
     4201 aaaaagaagc tcaatcacga tgatgaaata ggc
//
LOCUS       DAZKCY010000165         4148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000165 DAZKCY010000000
VERSION     DAZKCY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.167"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..388
                     /locus_tag="ACG8XC_002595"
     CDS             50..388
                     /locus_tag="ACG8XC_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192253.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHRHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            392..754
                     /locus_tag="ACG8XC_002596"
     CDS             392..754
                     /locus_tag="ACG8XC_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192254.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSMDNEWLADEKSAPSERDYPNESDGLLGVIPSKK
                     SQMSESKFTSIFNDVNLR"
     gene            976..2382
                     /locus_tag="ACG8XC_002597"
     CDS             976..2382
                     /locus_tag="ACG8XC_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFP9192255.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NEREKALVASERAEIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
     gene            2829..3803
                     /locus_tag="ACG8XC_002598"
     CDS             2829..3803
                     /locus_tag="ACG8XC_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9192256.1"
                     /translation="MSKRGTIVNEWEKLNRISNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYVEILKF
                     HEGKGRIDFNPNKINQFLSGSMKNFIHDLFLEPHFSRADIACDIVDIPDDFVSQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRESFAESADDLKRELDTWLQGLDVTEVGAE"
     gene            3800..>4148
                     /locus_tag="ACG8XC_002599"
     CDS             3800..>4148
                     /locus_tag="ACG8XC_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192257.1"
                     /translation="MSDSIEKMTLKELADELGVTKNTVRNQLVNRGYDLSRYKENGII
                     MLDKELIGVARSIYRSKTSQLTGNLPVNKAGNNFPLEKENEFLREQLVEKDKQISELT
                     EMIRDQQKLTLLQQ"
BASE COUNT         1445 a          601 c          937 g         1165 t
ORIGIN      
        1 aaaaaaacga tcagttgtta ctagaaaatc aggaattaaa aaatcgaaaa tggtggcaat
       61 tctggaagtg aggtgtgaca tggataaaat tgatttgaga gaagaaacag tcgatagaat
      121 tgaattgatg tatgaacggt tagctgatta tttgaatttg ccggatgaag ataattatag
      181 tgaaatgctt gaacgaatga tggatgttct caactcggtt atcaatgctg atctagcaaa
      241 atcttcagtt ttgactaatg aatttgttca taggcattta gcgcaaacaa ctgatgaatc
      301 agaaaaagaa ttttggttga aattaatgag tcatgttatg cagttaaacc aaatttccgt
      361 tattaattcc aaagaaaagg agatgtagtt tatgggaatg actttagcag atattaaagc
      421 gcaagaagaa aaattaaaaa aagaacaagc gaaacttcgt caacagaaaa aacgcttgat
      481 tgagaaagaa aatgctgcaa tgggcaaggc tcttcgcaag cgttttgggt ttgaatcttt
      541 tgctgatttt gagcggtggt tatcgtcaat ttctgacgat cagcaacacg aaaaatcaat
      601 ggataatgag tggcttgctg atgaaaaatc agcgccaagc gaacgagatt atcccaatga
      661 atctgacggc ttactcggag tgataccgag taaaaaaagt cagatgagtg agagtaaatt
      721 tacgtcaata tttaatgacg tgaatttacg ataagcgatt attttgcgtg aagcaaaata
      781 aagcgtaaaa ggagttgaga gcgtgacacg ctttcggctc cttttttcgt tgtcacttgt
      841 gacagcgaaa tccctcggag agcgtttgga attgtgacag taatgtcaca attaggaaaa
      901 cgttactctt tgacaaagag taacagaaag gtttacactc tttttattag cgacagcgcg
      961 aaggaggggt ttaacttggg gtttagtatt tcgttcaaaa agggaacaaa gaatacaagc
     1021 atttctcata ataatcgtga gttaactgat agccaaaaaa tcaatgactg gcataagcat
     1081 attgatttca caaaatcgga tgaaaatatc tatttggaac aaataaatat tcgtgaaaaa
     1141 tatgaagaat tattcggtga agccgtagaa aaatataatg caaagcagaa aagagccgat
     1201 agaaaaatcg aggactattt agcaaaggtt cgcaaggata aaaaaatgga accacagcga
     1261 gaatttatcg tgcaaattgg tactcttgat gattttcgta ctactcgtga tgatggttct
     1321 tctactggca tatcggaaca acaagcagaa caaaatcgag tgattgccaa caaaatttta
     1381 gttgagtatt tcaaagaatt taaagaacgg aatcctagtt tatccgtcta caatgctgtt
     1441 attcataatg acgaagtaag tccgcacctg catttgaata ttgttcctgt tgctgaaggg
     1501 tataaacggg gtgttcaaaa gcaaccaagt tttaacaaag ccttgcaaca gcagggttta
     1561 cgagcagata aagagaatgg tcgagtgatt tggaataatt ttaggaatca ggaagttgag
     1621 tcaattgaac gtttgatggc agattttggt tgggagcgtg agttagtcgg tactaacaaa
     1681 atcaaagatg ttcacgaata taaagaaatc atgagcgaga tttcagaatt gcgtcaaacg
     1741 gccgtaaaag agcgtgagag cgtttctgat gagttgaggt atatttctag gcaaagagaa
     1801 gaaaatgagc gtgaaaaggc tctggtggct tctgagagag cggagattga agaagagaag
     1861 aaaaatatgc tgaatgtaaa aaaagagatg gcggtcattt tccaaaaaga tatggttcct
     1921 ttagaaagtt tcaagaggct tcctgatggt tcgtatcatt tatctgggaa tgattttgtt
     1981 gatttgtatc aacgagctac gagagcaaca aatgccaaga gaaatgctga taacatgagt
     2041 gagcaatatt tacagatgct tgagcaaaat acagaattaa atgagtatgt agctgcattg
     2101 gaagaagagc gtcatttgtc tggtagaaaa caattagcag atttacaagt ggaaaataaa
     2161 aagctgaaag aagaaaatcg ctctttacgc cagcgtttag agcgattaaa aaacgcattt
     2221 caaaaatcga tcactcgttt gtctatccgt tttggtcttg cagaaaagga tatggggctt
     2281 tctgaggaat tagaagtatt agagcaggag aaaaccttat tttatcgtca aaacgtctca
     2341 cagcataaat tagagcgttc tgaagatgag atcgagtggt agttacgatg tgcagtcaac
     2401 ggtaccggag gcggtttgtg ccgaggataa ggagttgacg gctaaaccac tagcctatta
     2461 agctgacatt tgctaaaaaa tggccagtcc attttttggc caacctagcg ttagcgaagt
     2521 ttgggggcgc cctttttaaa gggtgcggtt ttatgttttt atatatttgc atagcaaaag
     2581 agaagtgcta ttattgagtt agcaaacaaa tgaactggaa aaagagaaaa aaataagtcg
     2641 agcaacgcta ccaacgttct cgacctttac ggaaactact tgtactagct ccgtaactca
     2701 tactcattat acgaatattt ttagatttct tcaagactag agattttttg gtaatgaatt
     2761 tttaagagtt acgtgaactt ttgaaaattc tttccagaag tctctttttc tttgaggagg
     2821 aacaaaattt gagtaaacgt ggcacgatcg tgaatgagtg ggaaaaactt aaccgcattt
     2881 ctaacaggcg gttaaagtat caaagtttga cccttccctt aaaaatgcag tggtcaattg
     2941 accgaattac catagtcgga aaactgaaag aaaatattta ttatcacact ccaaatgatg
     3001 tgttgatttt aaattttgaa cagcttatgc gtttgaacga aggcaatggt tatttaaaag
     3061 ctgttgggaa taatgggtgg caattattag accaacacga ggaaaatata gcttatgttg
     3121 agattcttaa atttcatgaa ggaaaagggc ggattgactt taatcctaat aaaatcaatc
     3181 aatttttatc aggttccatg aaaaatttca ttcacgattt atttctagaa cctcattttt
     3241 cacgtgcgga tattgcttgc gatattgtag atattcccga tgactttgtt agtcagtatc
     3301 gtgtagttga tcctgtgtcc tttaaaccaa tttatgggcg ttctggtaag ttagaaacgg
     3361 catattgggg tagtcgtgcc agtgaacgac aaattcgaat gtacaataaa aaattggaac
     3421 aagaaagaaa acgtaaaatt gttccgccgg aaataacgac atggtggcga ctagaattgc
     3481 agttacgaag gggaaaagca actgattggc atgagatggt tcatgaaagt ttggatagct
     3541 ttgctagtcc acattatttt ccgccaaata ctaaagttac tgaacggatt atggtgaaag
     3601 gtttgcttta tgatcattct gagtggggaa atctagctaa gaatacaaag aaaaaataca
     3661 gggatttgtt gaagcaagaa agtcaaaatg atgaattgac gaatcatttg cgagagtcat
     3721 ttgcggaatc ggcagacgat ttgaaacgag aattagatac atggctccaa ggacttgatg
     3781 taacggaagt aggtgctgaa tgagtgattc gatagaaaaa atgaccctaa aagaacttgc
     3841 tgatgagctg ggtgtaacga aaaatacagt gcgtaatcaa ctggtcaatc gtgggtatga
     3901 tctttctcgc tacaaagaaa atggtattat catgttagac aaggagttga tcggtgttgc
     3961 tcggtcaatt tatcggtcaa aaactagtca attgaccggt aatttaccgg tcaataaggc
     4021 aggcaataat tttccattag aaaaagagaa tgagttttta cgtgaacagt tagttgaaaa
     4081 agacaaacaa atttctgaac taacagaaat gattcgtgac caacaaaaat tgacattgct
     4141 tcagcaac
//
LOCUS       DAZKCY010000166         4136 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000166 DAZKCY010000000
VERSION     DAZKCY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.168"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..96
                     /locus_tag="ACG8XC_002600"
     CDS             <1..96
                     /locus_tag="ACG8XC_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9192258.1"
                     /translation="RNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(349..498)
                     /locus_tag="ACG8XC_002601"
     CDS             complement(349..498)
                     /locus_tag="ACG8XC_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192259.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            960..1172
                     /locus_tag="ACG8XC_002602"
     CDS             960..1172
                     /locus_tag="ACG8XC_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192260.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1450..3249)
                     /locus_tag="ACG8XC_002603"
     CDS             complement(1450..3249)
                     /locus_tag="ACG8XC_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFP9192261.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3373..3969)
                     /locus_tag="ACG8XC_002604"
     CDS             complement(3373..3969)
                     /locus_tag="ACG8XC_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9192262.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1487 a          726 c          705 g         1218 t
ORIGIN      
        1 cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt atttttgagt
       61 tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata ttaaatatta
      121 tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat aaaaaacgag
      181 atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga gacaatttcc
      241 aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca aacgtctttt
      301 attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt atctagattc
      361 gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat tttcgttttg
      421 tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt attgcatatt
      481 tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt cttgaaagtt
      541 tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga tgcaattaaa
      601 aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt ttcttttaat
      661 ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata aatcatatgc
      721 tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca acataggaat
      781 ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt atctaaaaag
      841 cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca aatacaaggt
      901 aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga gggaatgaaa
      961 tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta tctcatgaac
     1021 taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt gaaagttatg
     1081 accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta aaattctgtg
     1141 atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt cgcaagaact
     1201 cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa gaaaaagttc
     1261 agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt tagctattgt
     1321 tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca aacaaaaaac
     1381 gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt tttcattaac
     1441 gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat aagtgatttc
     1501 ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta tgataccatc
     1561 cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc atacatatac
     1621 ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac tatattccat
     1681 tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta agatgtagcc
     1741 cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg tcaaactttg
     1801 caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc gttcgacaag
     1861 gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt ttagcttatg
     1921 tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt tttcagtaac
     1981 tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta cccctgttaa
     2041 gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt cctgaacatc
     2101 catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt ctaaagtatt
     2161 ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt ttgtctgaac
     2221 agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa aaactgcaaa
     2281 aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt gtgggataaa
     2341 ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa tgatataaaa
     2401 aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa gcgtcatagc
     2461 aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac tttgtgccaa
     2521 aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa tcacaataaa
     2581 ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc gaatggctgc
     2641 tcgaaaagga cgatcccgta gaatcatttc cggtaaagca aaaatcagtc gaatcccaat
     2701 atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac gattagcaaa
     2761 aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag aattaaaact
     2821 taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa aagtaatggg
     2881 tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag agattggttc
     2941 tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat aaactaacag
     3001 taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt gagaaaaaat
     3061 tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa taaaacgggt
     3121 cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca gcaagacatc
     3181 acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc tacgtttcaa
     3241 ataattcatc ctatcacctg ctcttcacat tatagcgcaa atctaatgaa aaaggaaagg
     3301 gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat gactaatttt
     3361 acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa tcaaggccat
     3421 actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat atcgtttcac
     3481 tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa ctggaagaaa
     3541 aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc caatatattt
     3601 gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac gacgccctag
     3661 cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa atccagcata
     3721 agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga ttggaataac
     3781 tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg aatcacctac
     3841 taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat aaatcgtcag
     3901 cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc gagaggcagc
     3961 aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt actagaaaag
     4021 caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa gttcatcata
     4081 agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat aacttg
//
LOCUS       DAZKCY010000167         4095 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000167 DAZKCY010000000
VERSION     DAZKCY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.170"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..1460
                     /locus_tag="ACG8XC_002605"
     CDS             111..1460
                     /locus_tag="ACG8XC_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9192263.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1588..2178)
                     /locus_tag="ACG8XC_002606"
     CDS             complement(1588..2178)
                     /locus_tag="ACG8XC_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP9192264.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2325..3677
                     /gene="brnQ"
                     /locus_tag="ACG8XC_002607"
     CDS             2325..3677
                     /gene="brnQ"
                     /locus_tag="ACG8XC_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP9192265.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1186 a          747 c          791 g         1371 t
ORIGIN      
        1 attactcata aaaggtaaat ttctgaaaat tttggaatct tagttgtgtt tttcagaaaa
       61 ctctcttaat atggaagtaa ttaaactttt ttagttagga tgatttaaaa atgaatgaag
      121 agcaagaact gcagcgtgga ctgaaaaata gacatgtcca actgatttct attggcggag
      181 cgattggaac ggggttgttt ttaggttccg gaaagtctat ccagctagct ggcccttcga
      241 tattgctcgc ctatcttatt actggatgca tctgtttttt cattatgcgt gcgttaggcg
      301 aacttctgtt atcaaacacc aataaccatt cttttctgga ttttgttgca gaatatcttg
      361 gcaaaaaagc ggctttcatc actggctgga cgtattggtt ctgctgggta tcgattgcaa
      421 tggccgattt aacagccatc ggaatgtatg tacgctactg ggcgcctggt gttcctcagt
      481 ggcttcctga actgattgca ttaggtcttc ttctttgctt gaatatggtg gctgtctcat
      541 tgtttggtga gttagagttt tggtttgcat tgatcaaagt tgtagcgatc attgccttta
      601 tcctcgtagg agcttatatg atcctcaccc actatacgac agatattggc acagccagta
      661 tcacgaatct atggtctcat ggcggattct ttccaactgg tgcaaaagga tttatcttag
      721 ctttccaaat ggtaaccttt gcttttgctg ggatagaatt agttggatta gtcgctggag
      781 aaacagaaaa tcctgaaaaa gtgttgccgg aagctatcaa taatattcct atcaggatca
      841 tcttatttta cttaggatct ctttttgtca ttatggcaat ctatccatgg aacagcttga
      901 atccagataa cagtccattt gtagaagtat tttcggaaat tgggatcacg attgctgctt
      961 ccttgatcaa tttagtcgta ttaagtgcag cagcttctgc ctgcaacagt gcaatatata
     1021 gtacaggacg tatgcttcgt tctttagcgc aagaaggcag tgcccctaaa aaattcaaaa
     1081 agttaacaac acatcatgtg ccagggaatg ctttgacatt ttctactatc gtcattttca
     1141 tttctgttat cttgaattac gtgatgccta gtgaagtatt cacactagta agcagtattg
     1201 ccactacttg tttcttgttt atctggagta tgcttgtcta cacgcacatg aaatatcgta
     1261 aaagtatcct cggcaaaaaa gctcattcat ttaaaatgcc gttgtaccca tttagtaatt
     1321 atctggtatt tctttacatg gcctttgtgt gtgtcgtctt gttcctagga aaagatacga
     1381 gaatcgcttt attactaaca cctgtgtggt tcttgcttct attactaatc tatcatatga
     1441 aatatgaacg taagaagtaa gaaagtagtt gtgacagaag tggtcagctt cgagaaataa
     1501 gaaggaattt acgaaaattg cttcttaaat ttttgtgaaa tttcggctta tttcttggag
     1561 ataaaagctt ctgtcccagc ctcttttcta tttccccgga aacaaccgct gataataggc
     1621 atctgctttt ttataggaaa aaatccaaat accagataca aaaaaacgtc cgaataaccc
     1681 gatcaacggt acaaaaaaga caccggcaat caagcataag ttaatcatca tggagataag
     1741 acttcgacgg tcctttgaca aaactctcac gacatgggaa tctggtttat ttgctttcat
     1801 gatttgcctc atcaagatac caaaagaaaa actccacatc gtgtatacta agacataagc
     1861 catctcaggc caaaaagctt cagggttacg cccgacccaa ctagtaacag aaggacataa
     1921 agtcagccaa aagagcaaaa agatattcga ccagaccact cggtaattaa ttcgttctgc
     1981 catctgaaat atacgatgat ggttcaccca gatcaccgca acaaatataa agctgatggt
     2041 ataagctaat aatgtatttc ggatgaccca taaagaatga aagctagatg tattagggat
     2101 cttgatctct aatacaataa ctctcaaaac aatggctacc actccatcag taaatgcttc
     2161 taatctcgac tttgtcacga tgattcctcc tcagatgatt cagcgctttt attttatcac
     2221 ttacgttatt aaataaccat cgttttatat agggaatttt ctgaaaattg ttgcatattt
     2281 ttcgaaattt tgttactttt aaaaaaaccg taaaggagtt atgtatgaca aaaaaattat
     2341 catttaaaga ctatcttttt ataggctcca tgcttttcgg tctatttttt ggtgctggaa
     2401 atcttatctt tcccgttcat ttgggtcaag aagcaggcgc tgctaccttt tgggcgaatc
     2461 taggtttctt ggttaccggt atcggtcttc ccttcttagg ggtgattgcc attggtgtat
     2521 cgaaaagctc aggtgtatat gaattagcaa aacggataaa tcaaacatat gcgatggttt
     2581 ttaccatctt gctttactta gttatcggac cgttttttgc tttacctcgc cttgcaacca
     2641 catctttcga gattggttta gcaccattta tcgatcctag cagacagacg ctatatctag
     2701 caattttcag tattgtattt tttgtgctgg cttggtggtt ttcaagaaaa ccgacaaaga
     2761 tattagatta tgttggaaag ttcctgaatc ctgcattttt ggttttatta ggtgctttgc
     2821 ttctccttgc ttttttgaat ccactaggtt ctgtaaatca agcgcctatt cagccaaatt
     2881 atcaagagca tgcttttttc acagggttta ctcaaggata taacacatta gatgcgcttg
     2941 ctgctttagc atttgggatc atcattgtca caacaatcca aaacatgggt gtaacaaaac
     3001 ctgctgaaat cgctaaagac acgatcaaat ctggtgcgat cagcattata ttgatgggaa
     3061 tcatttatac tttgctcgct tatttaggtg ctatgagctt aggtagcttt gctctaagtg
     3121 aaaatggcgg gatcacgctg gctcagattg ctgatcatta tttaggtact tatggcagta
     3181 tcttattagc atttatcgtt atattagctt gcttgaagac cggtattggt ttgattacag
     3241 cttttgcaga aacattcaca gatttgtttc cgcaaagaaa ttacgcattc tttgttgcac
     3301 ttgctagtat ccttccttgt ttagcggcaa acgtaggctt gacgaatatc attcaatttt
     3361 cgttgcctgt tttgatgttc atctatccat tagcaatgac actgatttta ctagtgttag
     3421 ttggtccttt gtttaagcag cgtacggctg tttatcgaat gactacttat tttacattga
     3481 ttgcttcgat ttttgatggg ttgaacgctt gcccagaaac tatcaaacaa acacctattg
     3541 ttcaaaatct tttatatgtt gcggaaagct acttgccgtt tttcaaattg gggatggggt
     3601 ggatcgtacc agcagttatc ggttttgtta tcggactgat ttggagtttc gctaagaaag
     3661 aagaagtagc agattgataa aagaggttgt gaaagagacg ttcagccttg agcaataaaa
     3721 agtaattttg aaaaatggct tctcacattt ttgcataaat ttgacttaat accgagaaac
     3781 tgacttttga acaccgttta ttgagaggtc gtgaaaaagc cgcttagttt caagtattaa
     3841 gaaatatttt gtgaaaatgg cttctcacat ttttgcataa atttgactta ataccgagaa
     3901 actggctttt gaacaccgtt tattgagcgt ctataaactg acctgagtat tagagaaaca
     3961 aaacggcaaa gtagctccat atactttgcc gttttgtttt gaactaatac cgaagagaag
     4021 ctgatcaaac aacatttctt ttataatata gattttagac atatatatga tataaaaata
     4081 gttttcgcaa aaaaa
//
LOCUS       DAZKCY010000168         4078 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000168 DAZKCY010000000
VERSION     DAZKCY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.171"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..727)
                     /locus_tag="ACG8XC_002608"
     CDS             complement(74..727)
                     /locus_tag="ACG8XC_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9192266.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1015..2289
                     /locus_tag="ACG8XC_002609"
     CDS             1015..2289
                     /locus_tag="ACG8XC_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9192267.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            2296..3909
                     /gene="guaA"
                     /locus_tag="ACG8XC_002610"
     CDS             2296..3909
                     /gene="guaA"
                     /locus_tag="ACG8XC_002610"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9192268.1"
                     /translation="MRRTERAAYRTKFYSNLSSPTRFDEEPKIIVLDYGSQYNQLITR
                     RIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVL
                     GICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVT
                     QAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTG
                     DWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVL
                     LRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDE
                     ATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLF
                     KDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANA
                     GLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKI
                     SVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         1273 a          820 c          728 g         1257 t
ORIGIN      
        1 tgaactaata taattattat tccaagaaca gaaaatattt gtatgaacat ttttggaaat
       61 atctttaatt ttttcatttt ctctccaatt tatatcctac atttcttata gtagttatac
      121 agtctaattg taattttttt ctaagttctt ttatatatac atctattact cgatcataag
      181 gagtttcttc gctatctttc catacataat caataatttg cattcttgtt aacacttgtc
      241 cattattatc caataaacat tttaatactt ctagttcttt tgcatttaca tctatttctt
      301 caccatttat tttagctgta tagctattaa aattaaccga tagatcctta tattcaaact
      361 tctctaaatg tccataattc ttcttaatta aagaatctat tctagctttt aagaccggca
      421 atgaaaatgg cttttctaca tatccgtcta ctaaattagt aaatgcatca attttatatt
      481 cttcatcact aaatgcagtc aagattagaa ttggtaaatt gcttttcttt ctaatttctt
      541 tcaacacttc taaaccattt ataaaaggta tctgaatgtc taagataacc aaatttatat
      601 cgctattaaa ttttgacaag gcttctcttc catctttagc ttgaataaca gtatatccaa
      661 attctgaaag atattcactt attccctctc ttatcatatt atcatcttca acagttaata
      721 ttttcatata tacctccact taagcctata cactagatat tatacagcat attatggctc
      781 tataatatct agtgttggtg acaccttctg ttgccaatac tttttctttt ttctaataaa
      841 tgcaaacaga caccttgaaa tcctttagat taaagggttc tataatattt tgtgttgttg
      901 gaaaatcgaa ggcgattttc tactaacact ttttcttcgt ttcaactcaa aaagagactc
      961 acttttggta aaatttagtc gaccaaaaca tcaccctacc aaaggagtcg ctctatggat
     1021 aatcatacta gaaaattact cggattaaca gataaacatt tattttttga tgaagaatgg
     1081 ctgattgaaa aagaatataa aggcgttcaa gcgcagttta ttaaagggag attggatgac
     1141 tcaccttccc attgtgaaca ctgtggatcg attcgtatta tccgcaacgg ttcctatacg
     1201 acacgtactc agatattgaa agtcaaagaa aagttgacta ttttagaatt aaaacgtacc
     1261 cgttttctat gctatgattg tgggcagacc ttttctgcga aaacggactt agttgatgaa
     1321 catcatcaac taacaaagga actgaagcag gcgattctca tagaactgta cgaaaatcaa
     1381 tcacgtaaac taatcgctaa gaagtacttc gtctcggatg ggacggtgac acggatcttg
     1441 cgtgaagcaa cgaagcatta ccagccacgt atgaacttcc ttccgacggt gttgtgtatg
     1501 gatgagttca aatcaatgaa gtctgtttct ggttccatga gttttatttg tgttgatggg
     1561 acaacccatc agctatttac gatccttgaa gatcgcagac tttacaaact aactcaatac
     1621 ttcctgcgct ttcctagaaa agcccgttta aaggtcaagt acctagtgat ggacatgaat
     1681 gccagctatt gtcagttgct taaaacagtc tttccaaatg cggagatcgt aactgaccgc
     1741 tttcatattg tccaacacat caatcgcagc ttcaatcaat tacgtgtcca gatcatgaat
     1801 cgttttcgaa cctcacactc tgaaaatcag aagaagtacc gtcggctcaa gcgatattgg
     1861 aaactcttac tcaaagattc cacgacattg gagccactca aacggcatta tcatcgactg
     1921 tttaaacgac caattagtca aacagaaatc gttgatgagc tgttaagcta caacgaagaa
     1981 ttgcgaactg cgtatcattt ttgtcaatta ttacgctatt atttcgttaa acgaggtacg
     2041 gaaggctttc aagaaaccct taaaacaatt tcatctacgt tacctcagtc gtttaaaaag
     2101 aaattcacga tcttccatcg ctaccaatcg ggtatttcaa atgcctttaa ggtgtcccac
     2161 tcaaacggcg tcactgaggg gctgaataac aagatcaagc tgattaaacg aattgcgttt
     2221 ggttatcgca acttttataa ctttcgggca cggatctatt tacaacaagg tctgatcttt
     2281 gaaaactaaa aaaatatgcg gcgcacagaa cgcgccgcat atcggactaa gttttattca
     2341 aatttgtctt caccaacacg atttgacgaa gagccaaaaa ttatcgtact tgattacggt
     2401 agtcagtata accaactgat cactcgccgt attcgtgagt tcggtgtttt ctcagagttg
     2461 atgagccacc gcattaccgc agaagaagta aaggcaatga accctaaagg aatcatcctt
     2521 tctggcggac caaacagtgt ttatgatgag aaggcctttg gtattgatga aaagattttt
     2581 gatttgggga tcccagtttt aggaatctgt tacggtatgc agttgattac ttataaatta
     2641 ggcggaaaag ttaaaccagc caaaaaccgt gaatacggaa aagcagaatt ggaaatcact
     2701 tctgcagaag cggccctttt ccaaaacact cctgaaaaac aaaccgtttg gatgagtcat
     2761 ggtgacctag ttacccaagc accgactgat ttcgatgtcg ttgctaccag caaagattgc
     2821 cccatcgcat cgatccaaaa tgcagaacgc aaaatgtacg gaatccaatt ccatgcagaa
     2881 gttcgccatt ccgaatatgg aaatgacttg ttgcgccatt ttacactaga tgtctgccaa
     2941 tgtactggtg actggacaat ggataacttc atcgacatgg agatcgcaaa aattcgtgaa
     3001 caagtcggtg acaaaaaagt tttattaggc ctttcaggcg gagtagattc ctctgttgtt
     3061 ggtgttcttt tacaaaaagc gatcggcgat caattaacat gtatcttcgt tgatcacgta
     3121 ttgttgcgta aaggtgaagc agaacaagtt atggaaagtc ttggcggaaa atttggcttg
     3181 aacattataa aagtcgatgc caaagaacgt ttcttatcaa aacttgctgg cgtgtctgat
     3241 ccagaagaaa aacgtaaaat cattggaaat gaatttgtat atgtcttcga tgatgaagca
     3301 accaaacttg ccggtgaaga aggcgtttct ttccttgcac aaggaaccct ttataccgat
     3361 gtgatcgaat caggaactga aactgcccaa accatcaaat cacatcacaa tgtaggtgga
     3421 ttacctgaag acatgcaatt caagctgatc gagccattga acacattgtt caaagacgaa
     3481 gtacgtgaat taggtaccca attaggcatg cctgacagca tcgtttggcg ccaaccattc
     3541 cctggaccag gcttaggtat tcgtgtcctt ggtgaattga cagaagaaaa attagaaatc
     3601 gtccgtgaat cagacgctat cttgcgcgaa gaaatcgcca atgccggttt agatcgcgac
     3661 atctggcaat acttcaccgt cctaccaggc attcgctctg ttggggttat gggagatggc
     3721 cgtacgtatg actacactgt aggtatccgt gctgtcactt ctattgacgg catgaccgct
     3781 gactttgccc gtatcccttg ggaagtcttg caaaaaatca gtgtccgtat cgtaaatgaa
     3841 gtcgcacacg ttaaccgtat cgtgtatgat attaccagca aaccacctgc aacagtggag
     3901 tgggaataaa gagccaacat aatatggatg aggaatacct ataaatcaac attattcaat
     3961 tactaaagtt cctgattttc gtaaaaaata agatggtttc ctaccatttt ccctaccaaa
     4021 gcccttgtta gattaactac taacaagggc tttgctttta ttcactacct ttattttc
//
LOCUS       DAZKCY010000169         4031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000169 DAZKCY010000000
VERSION     DAZKCY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.172"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(84..542)
                     /locus_tag="ACG8XC_002611"
     CDS             complement(84..542)
                     /locus_tag="ACG8XC_002611"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP9192269.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(713..1858)
                     /locus_tag="ACG8XC_002612"
     CDS             complement(713..1858)
                     /locus_tag="ACG8XC_002612"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP9192270.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1871..2788)
                     /locus_tag="ACG8XC_002613"
     CDS             complement(1871..2788)
                     /locus_tag="ACG8XC_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFP9192271.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3216..3779
                     /gene="efp"
                     /locus_tag="ACG8XC_002614"
     CDS             3216..3779
                     /gene="efp"
                     /locus_tag="ACG8XC_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9192272.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1265 a          756 c          769 g         1241 t
ORIGIN      
        1 aactcttttc agacaataaa tatttaataa aatacgaaga taagatagct atattgcttc
       61 tcatctttac gttcttttag aaactatgct tctacttcag accattcatc gcgttttgct
      121 aaaaattcac gagccaaagc ttgtgcacct tctagtgtat ggctggcagc ccagccacat
      181 tgtgtttcat tgcttgcagg tacttcagta gcttcaagaa catctttcat tgttgcttca
      241 agaacatcta aaatgtcttc gtagctttca tgattcaata cagtcaagta aaagcctgtt
      301 tggcatccca ttggagacca gtctacgatg taatctgcgt gattgcggat tagttcggca
      361 gtcaagtgct caagtgaatg tagacttggc atgtccatgt gttctttatt tggttgttta
      421 aagcggacgt cgtatttaat gatgacatcg ccatgcgcac ccgtttttcg atcagctaca
      481 cgtacataag gtgctttgac ttttgtatgg tcaagattaa aactttcgac attcattctc
      541 atttgtaaga ctcctatctg tttgttcatt cgttttgatg cttttcacta aagaattatt
      601 tagtgaaatt tacaaaaacg tctttctact tgattctaac acttatcgtt tccaaggtgt
      661 agcaatctga aatatttttt agatttttct gctaagtcgt ttattgggac ttctagttct
      721 tgattctgtc aaatgcctga gctaaatcgt ttatgagatc ctgaccatac tcaattccta
      781 cagaaagacg gatcaagccg tcctttatgc cagcctcttc tcgtttttct tttggaatag
      841 acgcatgagt catgacagaa ggaatttcca ctaaactttc tacgccacct aaactttctg
      901 ctaaagtaaa tagttggagt gattcaacaa atggaatagc agattcttca ttttttaaag
      961 taaaagaaat cattccgcta aagccgctca tctgctgctt agcaactcca tggagttcat
     1021 gatcgggtaa tcctggatag tagacctttt ctactgctgg atgagaaaat agaaaatcag
     1081 ctaccacaaa agcatttttt tgatgttcct ccatacgaac acttagtgtc ttgattccgc
     1141 gctgaagtaa ccagctatct tggggaccaa gtacggctcc gatagcattt tggtaaaatc
     1201 cgatctgatc ggctagatct ttatggttag aagtcactaa accagctact acatcgctgt
     1261 gtccgcctag atatttggta ccgctatgga caacgatatc agaaccgaga tctaaaggtc
     1321 tttgaaaata aggagtagca aatgtattgt cagcaataac tatcaatcca tgttgatgag
     1381 ccagagtggc acttttttct aaatcagtaa tctttagaag cggattgcta ggtgtttcaa
     1441 gataaagtgc ttttgtattg ggtttgatgg attgttctat tttatcaaga ttactcgtat
     1501 ctatgatcgt gtactcgaga ccatttttag taagaacttt gtcaaataga cggaatgttc
     1561 ctccatagac atcatctcct aataagatat gatcgccagc ttgaaaaaga gaaaaaacgg
     1621 catgaatccc agaaagacca gaagaaaaag cgaatcctcg aacaccgcct tccaaatcag
     1681 caatcagttc ttccaatgca aaacgagtag gattaccaga tcttgaatat tcatattgtt
     1741 taggctggcc gacgccatct tgccgataag tggatgtttg ataaataggt acactaacag
     1801 ctcctgttgt gggatcttca ctgatgcctc cgtggatcaa ttttgtttga atgtgcatgt
     1861 ttctttcctc ctataagtaa atgttttttg ataaatagcg gtctgctgcg tctggaaaaa
     1921 tcgtaacgac acgatgacca ggtggtaaac gacgaatttc tcttaatgct gctgcaaaag
     1981 ccgcaccact cgaacttcca actaatagtc cttgttctcg tgctaactgc ctagtatagt
     2041 taaacccttc tacatcagag attgtctcga tctgattgat tgaaagggga gaaagaaacg
     2101 gcggaatgaa ttctacaccg ataccctcaa tttcgtgagg ggctggatca ccaccattta
     2161 acacagagcc ctctggttct acaccgatta ttcgaatgtc gggatatttt tctttcaaaa
     2221 aggtagaagt tccagcaaag gttccaccac tcccaattcc tgctacaaaa ctatggatat
     2281 tttctttgat ttcatgaaag atttctggcc ctaacgtatg atagtaagca gcaggattat
     2341 ctttattttc aaattgaaga ggcagatagc tgtttgatat ctcctctgca agttttttac
     2401 ttttgctgat ggcaccgatg attccttgct cactaggaga atggatgatc tttgctccca
     2461 atgctttcat caattgttgt ttttctaagc taaacttctc tggaacaaca aaaaccgttc
     2521 ttaatttgta tgtgagtgcg gccagagcga tcccaattcc tgtatttccg gcagttggtt
     2581 cgatgatcgt cgttttttca ttgatttttc cttgggcaaa tccttctttg atcaaataac
     2641 tgcccaatct atctttgata cttcctccag gatttaaata ttctagtttt gcataaatag
     2701 cagaaccttt aggcatagaa tagtgctgat tgtcaaagcg ataaagcggt gtggaaccaa
     2761 tagcttctgt aattgtcgag ataatcatgt gggcctccta aaaaaagata ataaaaaaag
     2821 gagctgacta ttaaatcagc tccttgaaag acatagagaa aaattgagta gataaaaaga
     2881 taagttacct ggtactcatt ctatgaaggg caattaatag acatgacaaa tagtatgctg
     2941 cttttatgca ccatacaaca gcaacgattc aattgttttt ggttgctgac tgtcatgtgt
     3001 atccctccaa gtaaaatttt tattaacgat agcatgaaaa aaaataaaag tcaaaggctt
     3061 gaaagaaaaa aatatttcga tagcatcgtt tacccgtgtt tatttttctg acaaataggc
     3121 atgagacctt aaaagataga gaatactcta gttttctggc cggttgtctg atacaataaa
     3181 gttgattatg ataaagaaaa gaaggagcaa aaataatgat ctcagcaagt gatttaagag
     3241 ccggtatgac tttcgtacaa gacggcaaac tgattaaagt tctggatgca agccaccata
     3301 aacctggtaa aggaaataca gttatgcgta tgaaactgaa agatgtccgt tcaggtgcaa
     3361 catacgacac aacgttcaga ccagaagaaa aatttgaaaa agcacatatc gacacaaaac
     3421 ctgtacaata tttgtacacg atggatgata cagctttctt catggatttg gaaacatatg
     3481 agcaatatga aattcctgta gaaacagtag aagaagaaat gaaatatatc ctagaaaaca
     3541 tggaagtaaa aatccaattt ttcggtagcg aagtcatcgg cgttcaatta ccaacaactg
     3601 tcgtattacg tgttgtggaa acacaacctt ccatcaaagg tgcaacagtc actggttcag
     3661 gtaagccagc gacaatggaa actggcttag tagtcaatgt tcctgacttc atcgaaacag
     3721 atgaattgtt ggaaatcaat acagcagaag gttcttatgt aaaacgtgcg tcaaaataat
     3781 tcgctaacgg attgataaga atttgaaaac agagcttgac ctcttgaaag gcagatcatc
     3841 ggatctgcct ttcaagaggt tttttgtgga atcagcaaat tgaaaaataa aaaagtattt
     3901 gattttattt atattttgaa agatgaatgg caaaagctag agaaagaaga aactgcactt
     3961 atttatgaag aaattgaaat gattcataaa attgaaaata tccgaactat tccgaactat
     4021 attgaagcga a
//
LOCUS       DAZKCY010000170         4010 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000170 DAZKCY010000000
VERSION     DAZKCY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.173"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..284
                     /locus_tag="ACG8XC_002615"
     CDS             15..284
                     /locus_tag="ACG8XC_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192273.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            289..453
                     /locus_tag="ACG8XC_002616"
     CDS             289..453
                     /locus_tag="ACG8XC_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192274.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            446..748
                     /locus_tag="ACG8XC_002617"
     CDS             446..748
                     /locus_tag="ACG8XC_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192275.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            745..906
                     /locus_tag="ACG8XC_002618"
     CDS             745..906
                     /locus_tag="ACG8XC_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9192276.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            903..1208
                     /locus_tag="ACG8XC_002619"
     CDS             903..1208
                     /locus_tag="ACG8XC_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192277.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            1208..1564
                     /locus_tag="ACG8XC_002620"
     CDS             1208..1564
                     /locus_tag="ACG8XC_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFP9192278.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            1524..1769
                     /locus_tag="ACG8XC_002621"
     CDS             1524..1769
                     /locus_tag="ACG8XC_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192279.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            1766..2185
                     /locus_tag="ACG8XC_002622"
     CDS             1766..2185
                     /locus_tag="ACG8XC_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFP9192280.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            2182..2739
                     /locus_tag="ACG8XC_002623"
     CDS             2182..2739
                     /locus_tag="ACG8XC_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFP9192281.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            2736..3032
                     /locus_tag="ACG8XC_002624"
     CDS             2736..3032
                     /locus_tag="ACG8XC_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192282.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            3109..3522
                     /locus_tag="ACG8XC_002625"
     CDS             3109..3522
                     /locus_tag="ACG8XC_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP9192283.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            3831..3983
                     /locus_tag="ACG8XC_002626"
     CDS             3831..3983
                     /locus_tag="ACG8XC_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192284.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
BASE COUNT         1464 a          605 c          936 g         1005 t
ORIGIN      
        1 taagaggtgg aaaaatgaag atcgattatc tagaactaat taatgaaata gcgaattata
       61 aaaagggcga ggaattagac gtcctgagag acgtatatga tcaactcgaa gaagctggaa
      121 ttgaaggaat taagaatgat cgttcgagtt ggagtaagct cagatactat ttcgcacttt
      181 atatcgatac aacacaatta agaaatttag catataccaa attactattt gttgattgtg
      241 ttaaaggctt gcaaaaacat cttaatgaac ttgagcaggt gtaatcagat ggacctaaag
      301 acatttacag cacagattga actaatgcat caagaagctt taagacaaag tgtatcgtac
      361 gaagacaagt ggctcaacac gttccatggc ggacgtgaga gcgcacttga tcaagtactc
      421 aaattactga aaggagaatg tcgggatgga taagaaagcg gcaatgcagc gaattatcga
      481 attgacttat tcagaagatt ggcaaaatga caaagaagct gcttcagaag tgatgaggct
      541 tggaagagcg atgtgggcag acaagagcaa caagccaaga ccacgaaaaa tcgcaatttg
      601 gcacggtgac aaacttctag tgatagggac agctgaacag ttagcaagtc tcacaggctt
      661 gcacgagaaa atcgtgagga aaagagcaag gtgtggctac acagacgcta agaagagaac
      721 gtttagatac gtggaggaat catcatgaca acagaagaag tgattcaaat gcgtattcga
      781 agccttcagc gtgagattga cgatctggaa cgaacaaagg cagtgatggt caatgaaacg
      841 gcgagaaagg caatcgattt gcacatagag aatttaagaa gggaaatcca tcgattggag
      901 gaatgagcgt ggataagaaa gcagcaatga aaagaattgc tgaattaacc aagtcagaat
      961 cttggcaaga agacaaagaa atagttgcag aagtccaaaa gctcggtaaa tcaatgtgga
     1021 ctgaaaagcc caaacggaga acgccgagaa aaattgcaat ctggcatgat gaccgaattt
     1081 tagtaacagg tactgctgaa cagttatctg aaattactgg attaagcaaa aacattatct
     1141 gggatagagc taggagctta tggattgatt caaaaggacg acagtttagg tatgtggagg
     1201 agagataatg gatctcatta cacaatacag tgacatcatc ctcaagaaaa tcatgatgaa
     1261 gattcagaaa gataaaaaat caaaagaacg agctgaatta gttaagttag aaatggctga
     1321 aacaggagca ggagtgcgaa gtagcaggca ttggaaagca gcagcaaaca ttgaatttta
     1381 ttacaacgaa attcaaaaag ggttcgatca gatgcgtgag ctggatcggc aaacaaattg
     1441 gagcaagaaa cttcatcaag atcgtttcaa atttgtagag aagtataaag aaatattaga
     1501 agagtatttg aggaggacag caaatgataa aaaaactcgt tcaattcagc atggatttat
     1561 atgatatcga atcaggagca acactatctg tggaatcgga ccatctaatc ataaattttg
     1621 gtggaaagcg ccagattatt ttgtgggtag ttgatgatgt actgtttcca gaaattgttc
     1681 atgatttcga agaatcaaaa gcggttgagt ttgaaatagt gaaaaaagta atggaattga
     1741 ttgaaaaata cgaggaggac agcgaatgat accgaagttt agagcgtggg ataaacgaaa
     1801 gaacgtaatg agagatgtag ccgtcttgca ttttactaaa aacggcaaaa caaactttat
     1861 tgaatattgg ataaatccta ccgaattgaa atcatatcac gtgcgaaaca tcgacctcat
     1921 gcaatccaca ggaatgaaag ataagaatgg tgtggatata tttgaagggg atatagtatt
     1981 agtcagcgtg cgaaatggct tcgattactt agataataaa gtctgtattg tcaaaaattc
     2041 aatagattat tccggattag tttgtgccac tgttgatgaa gacttagagt atcaaatttt
     2101 taacacagag ctgtttgaag aatacacgta cgaagtcacc ggaaatatat acgagaatag
     2161 cgagttattg gaggaacagc gatgaataaa caggaagcaa tcaaaaagtt agaaagtatt
     2221 aaagcgatag gaaatgatgc aatagctgct tgctataacg agagtataaa ttcaggtatt
     2281 acgttaatga aaaaaataga cgaaccgcag aaaccagtta ttccacaact cgtggccggt
     2341 tggcttgaga aatctacgga cccttttaca aaagctgaaa aaatagcgta tttaatcaaa
     2401 tctaaagatg gtgattcata ttatttctgt gattggtttg tacgagatgg catagtgacg
     2461 caagagcaag gagaagaatt actcgcttgg gcaacaagac aatcatatga aacactattg
     2521 agcctataca acggctacga ggttgagaaa gagccgttat gggcaataaa gaatgccgat
     2581 ggaaactatc ttactaaatg tgctttatgg ggaaaagatg gagtaaatta tagttttgaa
     2641 tgcaatccat ctcatcgatt gcttttcact gataaagcaa cagcggatgc tgcagcattg
     2701 ttggtgaatg gaacagtgga agaggtggta gaaagatgaa actaaaagac ggattttacg
     2761 ctagcagtca cggtatcggc ggtttaatgc tagatatgcc gacaaagaac cctaaaacac
     2821 gcaagaaatc aaaattcaaa gtcggtgaca tggtccgctg tgaagcagaa gagttcgttt
     2881 atccgttcag aggatacgtt aaaaagatac tgtcaaactc agcaatcatt cgtattgaaa
     2941 atacaatgaa gtgtgatcgt tcgactgcaa aacacaagca ttatttagct gtagcgagat
     3001 tagttgacat ggaagtaatc aagagcaaat aaaaaaagcc ggatcgctcc gactgattca
     3061 ataaatccaa cacatttatt atatcacata aaggagcggt ttgacttgat gcaattgtta
     3121 cgagaggtag atttcaaaca gacaagatgt aatgcgagag atgtgctgaa gaactttcgg
     3181 cgtttggagc ggatggcagg tcgctctttg atagatatta agtcgccgat tataacggat
     3241 atgccgaagg caccgaagca tggcaataag gcagaagacg cgattattca gatgatggat
     3301 atagaaacgg agagagatgc gattctagcg gctttgatgg ctcttagtct gattagtcgt
     3361 cagatactct actacagctt ctgtgtgcca gatagcttct caaactacag aattagccgt
     3421 gaagtgggtt attcagaaag aagtatacaa cggatgaagt cggaagctct aatagagttt
     3481 gcagaagcat ataaacacgg aaaaataatt gcttataaat aatttggcgg ttttttggcg
     3541 gaatgatggc ggtttttaga catttaccag tgttattatg gtagtgtcga aagattagga
     3601 aacaggatcg acaaaataaa cgaagggagg caatctccct catcgtttta aacttcttct
     3661 tagatagaca gcaactaatt atgaagaaaa ggatgtgaat tcaactcctc ctaaattctt
     3721 catcggttgc tgtttatttt gattagacag cagcactcac aaaactaaac atgttcttgt
     3781 cgtctttcaa ctgctgctgt ctattaattt atgtattgga gggaagaaaa atgaaattat
     3841 cagtagaagg cactccagaa gaaatacaag aattgctcca agctattggt agtagcaagg
     3901 agcaaaaggt aagtgtttct aattcagata ttgacggtat ttgcaattgt ttaaacagag
     3961 aaaaaatatc taatccttac taagagaaaa tatgttcgtt aatgaatttc
//
LOCUS       DAZKCY010000171         3969 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000171 DAZKCY010000000
VERSION     DAZKCY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.174"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1624
                     /locus_tag="ACG8XC_002627"
                     /pseudo
     CDS             1..1624
                     /locus_tag="ACG8XC_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
     gene            1735..2922
                     /locus_tag="ACG8XC_002628"
     CDS             1735..2922
                     /locus_tag="ACG8XC_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFP9192285.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            3093..3482
                     /locus_tag="ACG8XC_002629"
                     /pseudo
     CDS             3093..3482
                     /locus_tag="ACG8XC_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
     gene            3479..>3969
                     /locus_tag="ACG8XC_002630"
     CDS             3479..>3969
                     /locus_tag="ACG8XC_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9192286.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVS"
BASE COUNT         1410 a          595 c          736 g         1228 t
ORIGIN      
        1 agattttttt catggaattc aaaatgggtg gcaagggcat tggattatta tctctgcgac
       61 atttgaagga atcagcgaag aagaatttga caatgagatt tgtgcttcca tttctttaaa
      121 ttctgaaagt caaacaatct tagaagaatt gatttcaaat gcagataaag gtgtaggtag
      181 tatttctcta tttattagac ctaacaaagc tattagaaag caattatttg atatttccac
      241 tgaattagac tctcatcaat ttaacgaatt tagaaattct attcgtctat ccgactatga
      301 attttactac acatctaaat caaatctaaa tttctgtatc aatgaaaatt atgataaatt
      361 ggtaggaaga ctcaatgaca atatagctag taatcctgaa gaagacgatg aagctctgtt
      421 gggctcaaga attgacatgt cagatatttt caagcatatt tcggttgttt atgttgacgc
      481 tcttcgagat gttcttagag aaatgaaaaa caatcgaaac ccagtcaaaa gaatcatgga
      541 aacaattgaa tcaaaaatat catcagataa tgttgagtca ttaaaaacaa ttatacagca
      601 attaaatgag actattacca gtgttcctga aattaggaaa attggcgaaa atattaatcg
      661 gcaactaaat agcataattg gttcagtgta ttctcctaat cttcttcttt cctcaactat
      721 gtcagatgat atgggttctt tagcaaaatt tctttcaatg aagccagaac aaaatatcga
      781 cttagactta ctagggttag gacatctaaa tatgatttat ttagctttga aaatagtcga
      841 atttgaagcg tgtcgttcaa gagaactact taatattatg ttgattgaag aaccagaagc
      901 tcatattcat catcatattc aaaaaactct gttcgaagga ttaaatcttc aaaaaaacta
      961 tactcagatt ctcatgacca cacattctgt acatttagct gaagtgtctg aaatatctcg
     1021 gatgaatgta ttaaaatcgt ttaatggcaa atctatcgtt atgaatccag caaataatct
     1081 gaactcattt gctaaagaaa agttaaaaaa gaacaatcta aatctaataa acgcagtaga
     1141 gagatattta gatgttaaaa gaaatggatt gcttttttct aaaggtgtta tcttagttga
     1201 aggagatgct gaagaaatac ttattcctca aatagtaaaa aaattatttg gtataaactt
     1261 agacgaactt ggaataggtt tgattaatgt tggtagtact tcgtttgaat atatcgctag
     1321 cttgtttgac gatgatagaa tacagagaaa gtgtgctatt gtaactgatt tagatgaaca
     1381 ggcaatccca tcagaacatc gattgtataa aagcactgca caagagaaag gtcaagcaag
     1441 aaaagaaaaa ttatcctccc tttttaatga aaacaattgg gttaattgtt tttatgctaa
     1501 tacaacattt gaaattgagt ttttagatgt gaataaagaa aatattctaa cttatgtcag
     1561 tccaattata gatgacgcat tcaaagataa aaaaacaata actgcttata aagaagaaat
     1621 acaaaggatt acgtccatat aattgtgtaa aagtgaagca tcaaaaaagc cattgatctc
     1681 agttaggagt acaatgtttt ttgcagaaaa catactcgaa taggactgat atcaatgact
     1741 caagtacatt ttacttttga aagcgaagaa attcaagcaa ttataaacga aagcggtgcg
     1801 aatgataccg caaaaacact tatgactatc atgtttaatc agctgatgga agaacaacga
     1861 aatcaatata ttcaggcaaa tgcctatgaa cgttcagaag aacggcaatc acaaagaaat
     1921 ggttactacg atcgttcgtt tacaactcga attggtacgt tagaacttca tgttccacga
     1981 accagagacg gtaagtttag tcctactatc tttgaaagat atcagcgaag cgaaaaagct
     2041 ttgatcgctg ccatgattga aatggtcatt tctggtgtct ccactcgcaa agtaactaaa
     2101 acagttgaac tgctaacgga tggtgcgact gtttctaaat cattcgtttc aaatctgatg
     2161 aaacagttgg atccatttgt ttttgagtgg agaaaccgga gcctagaagg ctcggaatat
     2221 ccatttttca tgtgtgatgc cctgtatatg aaagtaaggg aaaatcatcg tattgtctca
     2281 aaaggtgtat atattggtat cggcattgat tctgatggac gacgtacgat tcttggtttt
     2341 gatgttcaag acggcgaatc ggaagataat tgggataccg tttttcaatc gttcgttcaa
     2401 cgaggtttgt tcggtgtaaa actggtcatt tctgatgcac acaaagggtt agttaaggct
     2461 gttcgcaaga actttttagg tgcgagctgg caaagatgcc aagcccactt tttgagaaat
     2521 atttttgata agctgccaaa gaaagtttct tctgatgtaa aagacgagtt gaagagtatc
     2581 tttaaagcct ctgaacttga attgacacgt gaaagaaaag agcacttctt ggaaaaatat
     2641 ggttgtgatt caaaattaag tgctgcttgc gacattttag aaaacggctt tgaagatgct
     2701 attcaaattt tgtcttttcc tgaaaatatc cgccgaagga ttcgtacaac caatgtgttg
     2761 gaacggttga acgaagagat tcgccggaga gaacgagtga ttcggatttt tccaaatatc
     2821 aattccatca ctcggataat tggaacactt ttgatggaaa aagacactga gtggctggct
     2881 tctccacgaa aatatttaga atttaattcg aataacattt agcaccatga acctgtgcga
     2941 gtctcttctt tctgaggaga gacatcaaga gtcgctgcgc tactgctcct gacctctctc
     3001 ctcagaaaga aaggcatggc agttaggttc acaacgcaaa acattgtgct tttgatgagg
     3061 ttgaactttt acacaagatt gtggacttga ctatacaaag tgaacaatat ttagaaagaa
     3121 atttagcgat gttaaaacta gccaatcatg ttggtaaggg gtggtttgca attgagttag
     3181 gtgaaaaaat tgacttttta atcaaaattc ctaagtatat tcttaatgct attgcttttg
     3241 catgtcagga aatagtagat ctctcaattt ataaaaaaat tctactccat acaatcgact
     3301 tgtacgagga aactacagaa ttgtcagctt tatcagctag tttaaaaaca agtgatacag
     3361 ctgatttgaa tatagcaata atttctttat tagagtactt tggtaaagat gaggatgttg
     3421 tgtatctaat caatcagata gaaaattaca caaaaatact ttatgctgag gtttcaaaat
     3481 gacaaatatc ttcgaaaaaa tcgtaaatga tttaaattta gaacaaaaag atgttgtgga
     3541 aagtgaagat aacctttatg taaatgcatg tcctgggagt gggaaaacaa gggtacttac
     3601 aaggaaaatt gcttaccaag caattaaaca cgatggatca ctaaaaagaa taattgctat
     3661 cacatacact aatagagctg ctgaagaaat taaggaacgc ctaagtttac ttagtataga
     3721 tgatgatatt aatatttggg tggggacaat acatcagttt tgtcttgaat ttattattta
     3781 tccttttaaa atgaatctct caagagtatc aaaaggattt acaattattg atgattatac
     3841 tcaaagagct tacgtcagcc aaattaataa tatgctgaat ttaaatctca aaacgtatga
     3901 actgaacaaa attgatttgt cactttctca tgattttaaa attaatgaag ttcagtatac
     3961 tcaagtttc
//
LOCUS       DAZKCY010000172         3912 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000172 DAZKCY010000000
VERSION     DAZKCY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.175"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..975
                     /locus_tag="ACG8XC_002631"
     CDS             100..975
                     /locus_tag="ACG8XC_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9192287.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1079..1843
                     /locus_tag="ACG8XC_002632"
     CDS             1079..1843
                     /locus_tag="ACG8XC_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFP9192288.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1830..3272
                     /locus_tag="ACG8XC_002633"
     CDS             1830..3272
                     /locus_tag="ACG8XC_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9192289.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3283..>3912
                     /locus_tag="ACG8XC_002634"
     CDS             3283..>3912
                     /locus_tag="ACG8XC_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFP9192290.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIY"
BASE COUNT         1284 a          577 c          838 g         1213 t
ORIGIN      
        1 ttatgtaggg tgctactatt caattaggca cgacctaaga aagggttctt tcttaggtcg
       61 ttttttgtct ttaaaaattg tttggagaca aaaatatgta tgaaggagtc tgcaagttgt
      121 tttgttttat catatagagg aggtgaatta acggtgtatg tgatgaaaaa gctgaatcat
      181 aatgctgtat tgatcgatga cgatggaata gaaaaaattg cgatggggaa agggattggc
      241 ttttctgcta aagtgagtaa gccatttgat gttgaggatg ctgacaaatt atttgtatta
      301 gattctaaag aaaagatcaa acgattcagt gagatagtct cgcagattcc aatcgaattt
      361 atggattttg cggaagaagt gattgaactg ataacagcta ggatctcctc tccattagat
      421 tcaaatatct atattacatt gacagatcat atttattttg ctattcaacg aaaaagagaa
      481 gatagagatg tcacagcaat tatgttgcca gaaatgagat tcctttatcc aaatgaatat
      541 gatatagcag tagaagtggt cgagcgaatc aatcatcgat atcaaaccga gttagggtca
      601 aatgaagtag gattcatcac tatgcatatc attaatgctg agctaggaga gcctaatagt
      661 ttaaagagct taaaaatagt agaaatgacg accacgattt tgtcccatct agaaaaatcc
      721 tattcaaacg tttatgataa agaatcactg acttatcatc gcttaatggt tcatatcaaa
      781 ttcctggtta aacgcttgat ttatcaggaa gaaacgccag tagaaaatat tggttttttt
      841 actgaaacat ttaaacatag caaagcgtat tgtacagcac taaagattaa agatatcatc
      901 gaacagcaat ttcaacttgc cattcaagaa aatgaaatca tctatttggc aattcatcta
      961 gcaagaatct attaaaataa gggcgtaact atctataggc gcaaccaaat gacacatagt
     1021 aaatggacta tgattatttg gttgcgccta ttttagtaga aaaaagaagg agaatgaaat
     1081 gggaaaagtg atttttaatt cggatgattt tggctatagc catggtgtga attatgggat
     1141 tatggatgct tatcagcgag gaattttgac atcaactaca ttgatggcaa atatgccggg
     1201 tttcgagcat gctgtgaaac taagaaagga aatgcctagg ctaggtgtag gagtccattt
     1261 aacattgacg tgtggcaaac cactcctcaa aactgtcgat actcttatcg aaagcggaga
     1321 gttcagatcc ttatcttatt atcagcatcc atttaacatt gacaaagatc aactgtatca
     1381 ggaatggaat gctcagattc aaaaaattta tcatgcgggg atcataccaa ctcatttaga
     1441 cagtcatcat catactcata ctttggatga aaatcaggag gtagttgtta cattagcaaa
     1501 aaaatatgat ttgccggttc ggagcaactt tgaacgaaaa gatgaagttc gtcatgtcga
     1561 ttattttgag ccttgtttcg atgtcgtagg agaaactgat gaaaaaaaac gacaaatcaa
     1621 tacgagtgtg gaagaatatt tggcaagttt actcatgata ttgagagaag ataaaacgat
     1681 cgaagtcatg tgtcacgcag cgtatttgga tcaggaattg ttaaatggtt caagttttgt
     1741 gtatcctcgt acccaccaag tcgaattttt aattcattca gcatttgctc aaaaagtgaa
     1801 aaatgatcct ttgattgagt tagtaacata tgaggatatt taatagagga ggaataaaaa
     1861 tgaatgttaa atcaaatgtg cagaagcttg gaagagcgat gctacttcca gttgcagcga
     1921 tgccattagc gggattgatc atgcgtttat cagctgacga tatgctgaac atcccagtaa
     1981 ttggtgcagc tggaaatgcc gtttttggaa acttggatat cttatttgct ataggagttg
     2041 caatcggttt tgtcaaaaat aaggacaaag gaatacctgc acttactggt tttttagcga
     2101 ttgctacctt gaaaaaaggc ttagaaatta tggacccaga agtgaatatg gggatttttg
     2161 gtggaattat ttccggttta gtagcggcat ggacgtataa tcgctttaaa gaacaaaaat
     2221 taccagtagt tttttcttat tttgcgggcg aaaaattccc attgactatg gtgatgattc
     2281 tacaaacaat cgcagctgtt tttttgggct ttatctggcc gctcatccaa attgggattg
     2341 ataattttgc aaaactgctc gttgatatgg gagcattagg tgtggggata tttatgtttt
     2401 tgaatcgttt gctgatcccc tttggactgc accatgtgtt gaatacgtat atttattacg
     2461 atttaggaag ctatacatct cctagcaagg aagttttcag aggagagatg acacgcttta
     2521 tcaacggaga tcctcaggct ggactgtttt tatcaggttt ttttgtagta atgatgtttg
     2581 gtataccagc tatatgtcta gctatctata aagcagctta taaagaaaat aaagagatgg
     2641 tcaaaggaat catgggaagc ggtgcaggaa cttcatttat tgcaaatatc actgagcctg
     2701 ttgaatttag tttcatgttt ctttctccta tgttgtacgt gatccatgca ctttttgccg
     2761 gtttagcagg gattgtctgt tacttattca atatacgaat tggttttaca ttcggagcat
     2821 gtattgttga ttatctcatc aattttcgaa tcgctaccaa tgctattttg attcttccaa
     2881 tcggaatttt cttttttgcc ttgtattacg ttacttttta ttatttgatc aataagagaa
     2941 acattcagac attaggacgt gaagctaagg cagagtttgg caatgaagta accttagaag
     3001 aaacagagtt agggttagca agtaaaaatt atgaatatat ggcaacaaaa atgctacaag
     3061 cgtttggagg aaaagccaat atcctggatg tctatagttg taatacacgc ctacgggtag
     3121 aggtagtgga tcccaccatg gtcgaggaac agcgaatcaa gcaattaggg ataagcggga
     3181 tcattaaacc aactgaaaaa aattatcaaa ttatcatcgg cttagaagtg acttatgtta
     3241 tggcagaatt caataaatta ttagaagaat agaaaggaaa agatgatgat gaaaaaacag
     3301 attatcacta tagctggagg cgggagtacg tatacaccag gaatcgttca agccattttg
     3361 aacaatgaaa aacggttgcc attatctgaa atacgtttat atgatattga tgaagaaaga
     3421 aatatggata tgtacttgat tgttaaattt atgctgaaaa gaaaaggttt ttcaaatatt
     3481 cgcattcgtg caacggatga tccaaagctt gcttttacag gatgcgattt tgttttctca
     3541 caaattcgtg ttggaggatt ggagatgcga gaaaaggacg aaaagattcc acttaaacat
     3601 ggattagtag gacaagaaac gtgtggactc ggaggatttg cttatgggat gcgctcaatc
     3661 aaaggtttat tggaaattgt cgatcatata caagactatg cccccaaagc atggattttg
     3721 aattatacaa atccagagtc gatcgtttct gaagctgttc gtcgtaaata tccgaatgta
     3781 aaaatgatta atgcctgtga tatgactatt tcaattgaag aaacgattgc agtgaattac
     3841 ggttatgatc gtaagaattg gatcgttacg tattatggtt taaaccactt tggctggtat
     3901 acatcgattt at
//
LOCUS       DAZKCY010000173         3895 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000173 DAZKCY010000000
VERSION     DAZKCY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.176"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1073
                     /locus_tag="ACG8XC_002635"
     CDS             <1..1073
                     /locus_tag="ACG8XC_002635"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9192291.1"
                     /translation="YFIYANKILPLVIKGEIFMFNFDQERFLKTQAGAVATNEQISNI
                     LESIASDLEEIYFIGSGGVGILFDSTIEFARTRSTFPFHRVIAAEFMIHPNKTFSNKS
                     LVVIPSLSGTTKETLELVEYCNEMNVKTLALVGSPKTPLEKAATYSIYNDVLDDTSSE
                     NYYLQGLLIALFLMKQRNEIDINQYNTYIEDLKKIPEALVHAKEQAEPMAKSFAKKHK
                     DTSYHILTGAGDSFAEAYYYGMCILEEMQWIKTRPVHAADFFHGTLELIEAGVSVIIT
                     EGEDDSRPLTERVKNFVPKFTDVHVTVDTKDYDLPGISEDTRKIISPVVLATVFERIS
                     AFLEEERNHPLTTRRYYKQMEY"
     gene            1181..2050
                     /locus_tag="ACG8XC_002636"
     CDS             1181..2050
                     /locus_tag="ACG8XC_002636"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP9192292.1"
                     /translation="MKIAVICSGGDSPGMNSALLAIKKTIDSEPDSEVVFFKNGYAGI
                     FKECIASVSLKEVEVFKAGTLIGTARFEKMRDLESRKIIIENLNKMEIDNLIVIGGNG
                     SLKGAELLKNESELQVSVIPGTIDNDFPNTDYTLGFDSALNTIIRSIDDLTSTANAHD
                     RIIIMEVMGRGCFALPEYASIANNTYWVERDDKEYLHLICKKFESEFYSPILLLPEGI
                     SNKQKIIEFITKNLPKETKYLNLAYLQRGSSPTFYDRYIGYLWGQLAAKELIEGKSGI
                     LSQKNGNIMLIPW"
     gene            2065..2769
                     /locus_tag="ACG8XC_002637"
     CDS             2065..2769
                     /locus_tag="ACG8XC_002637"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9192293.1"
                     /translation="MIFDKVKEQIRKVYITTDRKSELLPSERDLAQEFSVSRPTIRKA
                     LASLEDEGIISKNEGRGYLVKPASEKYVDHELDSFIGFFQDAVQQGKKISSKIIQQTI
                     IPADSTISECLEIEQNTLVLVLERIRYIDDYPTCIAKSCIPLSVLPEIVEHDFAKESL
                     FSVLESRGIRLAYAKRSIEVTPPTDLENLYLDLEKNEPVFVFTSIGFAKNQKPFEYEI
                     SKYPAYKTKFESTVSK"
BASE COUNT         1308 a          679 c          669 g         1239 t
ORIGIN      
        1 ggtattttat ttatgcaaac aaaatattac cattggtaat caaaggagag atttttatgt
       61 ttaattttga tcaagaaaga tttttaaaga ctcaagctgg tgccgttgca acaaatgaac
      121 aaatttccaa tattttagaa tccatcgcat ctgacttaga agaaatttac tttattgggt
      181 ctggaggagt tggtattctt tttgattcaa caatcgaatt cgcgagaact cgtagcactt
      241 tcccttttca tcgtgttatc gctgccgaat ttatgattca tcccaataaa acattttcta
      301 acaaatcttt ggttgttatc ccatctttat ctggtacaac aaaagaaaca cttgagttag
      361 tcgaatactg taatgaaatg aacgtcaaaa cattagcttt ggtaggaagt cctaaaacgc
      421 ccctagaaaa agctgcaaca tattctatct ataacgacgt tttagatgat acatcttcag
      481 aaaattatta ccttcaagga ctgctgattg ctctcttctt aatgaaacaa agaaatgaaa
      541 tcgatatcaa tcaatacaat acctatattg aggatttaaa aaaaatacct gaagccttag
      601 tgcacgctaa agagcaagcg gaaccaatgg caaaatcttt tgcaaaaaaa cacaaagata
      661 cttcatatca tattttaacc ggtgctggtg atagctttgc ggaagcatat tactatggca
      721 tgtgtatttt agaagagatg caatggatta agactcgtcc tgttcatgca gcagacttct
      781 tccatggaac tcttgaatta attgaagcgg gtgtaagtgt catcattaca gaaggagaag
      841 atgattctcg acctctcaca gaacgcgtga agaatttcgt tcctaagttt acagatgttc
      901 acgtaacggt tgatacaaag gactatgact tacctggcat ttcggaagac acgcgaaaaa
      961 ttatttcccc agtcgtctta gctactgtct ttgaacgaat tagtgctttt ctagaagaag
     1021 aacgtaacca ccctttaact actcgccgtt attataagca aatggaatac taatcataaa
     1081 aatatgaaaa aggtgaccgc gactccattg cccatttgat tgcttcattt tctctaatgg
     1141 gagaatagaa tcgcagtcac cttttttagg aggatctaaa atgaaaatcg cagttatctg
     1201 tagtggtggc gactctcccg gaatgaattc agctttatta gcaataaaaa aaacaattga
     1261 tagcgaacca gattcggaag tagtcttttt taagaatggt tatgccggaa tatttaagga
     1321 atgtattgcc tcagtttctc taaaagaagt tgaggtattc aaagctggaa cacttattgg
     1381 aacagccaga tttgagaaaa tgagagatct cgaaagtcgg aaaattatta ttgaaaacct
     1441 aaataagatg gagatcgaca acttaattgt tattggaggt aacggttcct tgaaaggagc
     1501 tgaactattg aaaaatgaat cagaattaca agtatctgtc atcccaggga ctattgataa
     1561 cgatttcccc aataccgatt atacacttgg cttcgatagc gccttaaaca ctataatccg
     1621 atcaatcgat gatttaactt ctactgctaa tgctcatgat cgtattatta taatggaagt
     1681 catgggccga ggctgctttg ctcttccaga gtacgcttcc attgcaaata atacttattg
     1741 ggttgagaga gacgacaagg aatacttaca cttaatatgt aaaaaatttg agtcggagtt
     1801 ctactcgcct atattgcttt taccagaagg tatatctaac aaacaaaaaa tcattgaatt
     1861 tattaccaaa aatttaccaa aagaaaccaa atacttaaat cttgcgtatc ttcagcgtgg
     1921 atcatctcca actttttatg accgatatat tggctatttg tggggtcaat tagcggcgaa
     1981 ggaattaatt gaaggaaaat ctgggatatt aagtcaaaaa aatggtaata taatgttaat
     2041 accttggtaa aaggatggat tactatgatt tttgacaaag taaaagaaca aattcgaaaa
     2101 gtgtatatca ccactgatcg gaaatctgaa ttattgcctt cggaaagaga tttggcccag
     2161 gaattttcgg tgagtcgtcc aacaattcgg aaagctttag cttctttaga agacgaagga
     2221 attatttcta aaaatgaagg tcgcgggtat ctagtgaaac cagcttcaga aaaatatgtt
     2281 gaccacgaac tcgactcttt tatcggattc ttccaagatg cggttcagca aggaaagaaa
     2341 atcagttcta agattatcca acaaaccatc atacctgctg attcaacaat tagtgaatgt
     2401 ttggagattg agcagaatac tttagtgctt gtcttggaaa gaattcgata catcgatgac
     2461 tatccaactt gtattgcaaa aagttgcatt cccctatctg ttttacctga aattgttgaa
     2521 catgattttg ccaaagaatc cctgttctca gtattagaaa gtaggggaat tagattagct
     2581 tatgccaaac gctcaattga agttacaccg ccaacagatt tagaaaacct ttatcttgat
     2641 ttagaaaaaa atgagccagt ctttgtcttt acaagtattg gatttgctaa aaatcagaaa
     2701 ccgtttgaat atgaaatatc taagtaccct gcttataaga caaagtttga aagtactgtt
     2761 tccaaataat ttttaaatat gcttaaatgt ataaagagcc tcgcggataa cagataaagt
     2821 gtctttccgc aaggctcttt ttctacttcc tctttatctt aatccgctaa gtagcctaca
     2881 gtccaaacat tatcatctaa ttaaggtgtg tggaggtact cctctaccct aagtcaagga
     2941 tagtcgacat agttgttaat aagtcttcaa agcaagcctg aacctttttc attaggtgtg
     3001 cttacttcac atatgacgct atcaaaaatg atagcacccg ttgaatcaac caattttctc
     3061 taattcatat ccccattcaa acaactatat tttaaaaact tttttaatct ttgattatgt
     3121 gtagcaaatt tgtctaattt ttatttcttt gcaaaatttt tccaaattta ccagataaaa
     3181 gtaattcata ttcatgattg agtcctttac caataaaacc tatttcgcaa aaattatttg
     3241 taagcattaa ggattttctg ctgactttca ctggaaggat aacccaagaa ttctgaccaa
     3301 agtgaaagat taagaaccat ctttgccagt ctatctatgt gctattgtaa ctgtcaacat
     3361 catgcgggca gttattatta tttatttgaa taatattttt attataaatc agttttttag
     3421 attgtgcata atcactaatt gctgtgatta attacagaat atggctctct gggaaaatgt
     3481 gcgcgtcatg tattcaattt taaattctaa tttgtacaaa atgaatcgtc acgactcgat
     3541 ctgacgcaac tccaaatggg ggcgccgacc tgtaccaagt acagtggcat gttctttaaa
     3601 catttgaagc aaaatctgtc catcaaaaac ctgtattttc aaagtgaaca aggtgcgcta
     3661 aatcaggaaa tacttacact gatcatttct taatttacct acttaaccaa aatttaaatc
     3721 aatatgaaag caacactttt caaactaaag aggtcttttt accgcttgat gtttgcttcg
     3781 ataaaaaagt tgatgtcgga ttagttttca ttttaataaa acgtactatt cctgaaaaaa
     3841 atttatccac tactaagtat tcggattttt ctaattttta acaaaattta tttcg
//
LOCUS       DAZKCY010000174         3871 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000174 DAZKCY010000000
VERSION     DAZKCY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.177"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            406..1899
                     /locus_tag="ACG8XC_002638"
     CDS             406..1899
                     /locus_tag="ACG8XC_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFP9192294.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2034..2330
                     /locus_tag="ACG8XC_002639"
     CDS             2034..2330
                     /locus_tag="ACG8XC_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFP9192295.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            2431..3282
                     /locus_tag="ACG8XC_002640"
     CDS             2431..3282
                     /locus_tag="ACG8XC_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192296.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            3303..3515
                     /locus_tag="ACG8XC_002641"
     CDS             3303..3515
                     /locus_tag="ACG8XC_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192297.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1372 a          567 c          788 g         1144 t
ORIGIN      
        1 gtgaaaagag gtaaacttaa ttctatagaa accacagata attcactatt tacaaagtag
       61 caaaagcagt ggtactcttt aaacagaaaa attacataaa aaaaagagac ggaaataatt
      121 gattttagca gaatcaatta tttctgaatc acatggttag gacatgtgtt cacgctcgtc
      181 tcaagaatat gtaagtagct accaactact tatattaaaa gttgatcgga taccttttcc
      241 gattgccttt attctaccaa aaaaaggcga gcaactcaac agaagaacgt ttatttagtg
      301 tacttaaatt aaatattctt aaacactgaa gagagaacac atactatggc tgagattaaa
      361 gctaaactgt gttctctttt tgtttgagaa aggacgttac aatcgatgtt gagcgaaatt
      421 tattcagcta ttcttaaaaa cggcttacgt acatacaaat atcgaaacag caccatgaaa
      481 cctgcaggtc atattgattc cagtaggagt ggtgctatct ttgcgtaccg tagcaagcaa
      541 ttaatgaatg ttgggcgtgg tatggtcatt acttcggaag aagctatttt agagaacgaa
      601 aagaaactaa cgcattggac acctaatacc tatcgctttg gtacttatgc ggatgattat
      661 cgaacagtgg ttaaaggtca ttctgagaaa aacttaagtc aaatcaacac atttgtagtt
      721 gatatcgatt caaaagagaa ccatcaaggc gaaattattt tagcctgttt agatcaagtg
      781 ggctacatgc caacgctaat tttagaatca gatcatggtt atcaagttta ttttgtacta
      841 aagacaccag gctatgtcac aaagaaaagc aatttcaagg tgatcgatgt agcaaaaaaa
      901 atttcaaaaa caattcgtct acagttggct gaaaaattgt ccggagtgga tctaggatgt
      961 aaccacttcg gaattgctcg attcccacaa gaaaataata ttgtttttaa agaaatgaac
     1021 tatcagtatt cttttgctga ctggattcaa tggtcgatga agcttgaatc agatcaaaaa
     1081 acaaaagaag atcgagaacg aaaactaatt gtctttcctg agaaaaaaga aattcgtcaa
     1141 gtagatgaac cgtggttcga tatgttactt cataaagcaa acatcgtcgg cggagaagga
     1201 cgtcttggtc gaaataatgt gattttcacg ctttctttag cttattactc ttcaggttat
     1261 gcattggata cttgcgaata caatatgttc acattcaacg aacggttaca ggaaccgcta
     1321 tctgaagcag aattagtaaa gattgtacgt agtgcttatt ctggtaatta tcaagcagca
     1381 agcagagaat tcattattga attgtgccag caatgggttt caccaacctt aaaaaataat
     1441 gatctgttca ttcaacgacg tggctggtgg aaatttaaaa agccacgaga tcaaagaaaa
     1501 tatagtcata aaaatgaatg gaaagaagat ctattggctt atctaagcga aaaaagtctt
     1561 gctagtgagc cttatttgaa cctgtcaaaa aaagaattgg cttcacaact aacgatgccc
     1621 aagcgaacac ttgataagat cttaaatgag ttacgagagg agaataaagt cttctacaag
     1681 gtaacacgag gaagaaatgg cggaatgatc attgcttcag tcaaaacgtt gttcgcgagg
     1741 gttattcaac taagtaaaga aaagagacta gattattcaa caaggatcac agaggtattt
     1801 ggactcaacg agagcttagt agaagagaca tttagacaat tatcaacccc cttgaaccct
     1861 cttaaagaag tgaatctttt tgaagtagat acaggttgaa aaataaaagg tgcaaattgc
     1921 cctatacaat atatctatga tacttctgta ggtgttgctc cctttgccta gctacgtgta
     1981 ggtggtatac ttataactgc gtatgcagtc ttatgtatca tggaggaagt tgtatgtcgt
     2041 taaaaaatta tgtctattca catcctgtta atgtgtttat cattggtcgt ttgggattat
     2101 ctgtggaagt gttctgtgag ctttatggtt ttaagcaggg gactttgtct tcttgggtta
     2161 ccagggaaaa aacagttgct tctttgccga ttgagtttct tcatgggttg tccttagctt
     2221 ctggggagac tatggatcag gtatatgatt gtttgtgtgt gttagagcag gagtatattg
     2281 agtataaaat agctaacgag ttaagaaaaa ggaagaagta tatccagtag tagtttttat
     2341 ttacttaaaa aattggttca ttgagcaacc tatcgctgct aaagtagcaa aggaaaacgt
     2401 taaaggaaag aatttaggga agaaatcgaa atgaaatcgg acgactggat tgaacacacc
     2461 tggcttcctc tgaaaagaaa tagcaaacga aagaaatttt gggtattgca attatggcca
     2521 ttaggaaaac tttgtagtta cggggcacga cttattcttg atttaaccgt ttactcgatt
     2581 ggagtatttg gaacggctta ttacttgata aatgatccca ataggattgc agcatcagac
     2641 atttttacag tgctaatgac gttgattttt gtcatgctat tgtctatttt tactcaatta
     2701 gtcatgcaat ttaccaggca aaaattaaca gcaggaaaac ttttcaaaag cctattcgat
     2761 ttaattgttg taaatgcaat tctagcgact ttaataaaac aaattgaaca atctggaaga
     2821 acaacaaaag gagtgaagga ttttttattg acgtttattc actttgtaaa tggacacatg
     2881 tatttgtttg aagtgggtat tattctcttt tttgtcgggt atgtatccac gaaacttatt
     2941 aaacgttcaa ttgaaaaaga aattgaaatt gacctgattc cgacacttct tggttatgag
     3001 tacaatgttg attttggaag atatgacgaa ttgatcaata cggatatcgt tcacacgagt
     3061 atccatgaat ctactgttaa gggcatcaga tctattttaa aaaatggcca ggaaatgaaa
     3121 gcaatagatc gaagaaaaga gatctacaat ttttatgaag tcgaagcaag cctacgatat
     3181 ggcatcggaa aattgtggtt agatggaagt aatattcgtc aaaacaaacc atacattttt
     3241 tggaacagta gtgatttaga acttccaatg aaagataaat aattatggag aatcgtttga
     3301 aaatgattcc tctatacagg attgtgctta tagttggcat ggagttaaaa accttaaaga
     3361 aggagcagtt attcaaagtg aaagaacgtc ataatcagtt actatgtagt aaaacgggta
     3421 cgatgaacat tgaataccga acagaatatt tagaattaac gaatcaaact gattttttgt
     3481 tgattcgaaa gcaacaagag caagaaaaaa attaatggct acttatagcc aattgattga
     3541 tgaatgggaa caattcaagg aatctaaaat tccagaacgt ctctaaaagc ctttatttgt
     3601 aaagagcata aggaaacgtg ggaacacttt ttaaaaaaag tgttccaata ggctgtgcct
     3661 attacgtttc tcaaaaataa atgtgggaac aaaatattta aaaataggat agtgaaagga
     3721 tagtgaagga tagtaaaagg agggtagtag gatagcaaaa aggataggct ccttttcgct
     3781 atccttttta aattggagga attaatatgg aagtaagaat aagaaatgtg gatgagaaaa
     3841 tcgtaagaga aattgatcga agatgtgacg a
//
LOCUS       DAZKCY010000175         3863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000175 DAZKCY010000000
VERSION     DAZKCY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.178"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..418)
                     /locus_tag="ACG8XC_002642"
     CDS             complement(128..418)
                     /locus_tag="ACG8XC_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFP9192298.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(554..733)
                     /locus_tag="ACG8XC_002643"
     CDS             complement(554..733)
                     /locus_tag="ACG8XC_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192299.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(730..1080)
                     /locus_tag="ACG8XC_002644"
     CDS             complement(730..1080)
                     /locus_tag="ACG8XC_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192300.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(1073..2398)
                     /locus_tag="ACG8XC_002645"
     CDS             complement(1073..2398)
                     /locus_tag="ACG8XC_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP9192301.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            2848..3462
                     /locus_tag="ACG8XC_002646"
     CDS             2848..3462
                     /locus_tag="ACG8XC_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9192302.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(3627..>3863)
                     /locus_tag="ACG8XC_002647"
     CDS             complement(3627..>3863)
                     /locus_tag="ACG8XC_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192303.1"
                     /translation="FMYRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGISI
                     TNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT         1193 a          740 c          602 g         1328 t
ORIGIN      
        1 taaacattac taaactgtct atattgttac taataattcc cttcttatag tgcattttct
       61 tagaaaaaaa caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg
      121 attgatatta tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt
      181 ctttttctaa agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc
      241 ttaaatcata aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc
      301 ttgcaaccgt tccttgtggg taggcatata actcacaaaa ttgttctaca gtgattccta
      361 aattcttgat aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg atagacatgg
      421 tgcgtaagcc tactttcttt tattattgcg tttgcaataa tagtttaaca cacgcattat
      481 tctagtatct actcctattt gtaaaaacaa aaaaggattc atagagataa ctctatagaa
      541 tccgtaaaat accttatata tataacggga agatataaaa cctaaaatta tgataacctg
      601 ctgcagcttc tacatagaaa gagaagcgct caacaccaac ctcatttatc aaacgttggc
      661 cataacccca aatctcttct gatagctgat caaaggttat atactttagc tcatattgct
      721 ggataatgtt cattctaatc gactccattt attatcctgg taaaattcga tatgacgtat
      781 ttcgtcgtag tgaattttgt cttttccaac gtagatcccc agctcatcac tacctaagat
      841 cttgccaatg atatcttctg gatagtatcc ttcagcgttt cgttcttctt tttgaataga
      901 tactgatcta ttttttatga ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat
      961 ttgttctttt gctaaatttt gactggttcg ttgttcattg tcggcatcaa tttcttcagt
     1021 atgatcagat aaataaaacc ctgcccactt taaacgtcca cgatcattgt aatcaatcat
     1081 ttttaattcc cccatttccg cccgcatgac caccaacaag tgtgcttcta gtaatgcttc
     1141 tagcgccttc taacattgaa gaagcatgta caatcgacgt aaaaccatat ttttgccgaa
     1201 tcttgtcaat aattttatct aaattttctt cgtcaatttg cttttgtgga tcactaaata
     1261 aatccagctg aagcgaatcg gtgtacacaa gcttgctata ggttattcca acatgcctta
     1321 cttcttgtcc ttcataatac ttatcaaaca agaaaagaca ataattctgc aattcctttg
     1381 tattatctgt gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg
     1441 acgtgccaat gtgtaaatgg acacaacctg ttttacagtt atgctgccta atgcgtatcg
     1501 ctacttgttc cgccatttct tttaccacca gctcgatttc atttcttcta gtataatctc
     1561 gtggtaacac ttgtgaattt ccatatgatt tttcctttgt cggttctggt gggattgcta
     1621 tatctgttcg atcgatccca ttagcatgga agtacagctg taagccaatc acacctagtc
     1681 tattcttaat cgtataagga ttccagttag caagatctct aatgctcata atccccatct
     1741 gattgagtct ttttttcatt cgagagccaa ttccccaaaa gtccgtcatt tcaggaatat
     1801 tccatacttt ttcaggaacg ttttcgtacg tccattccgc aacaaatcct tcattatgct
     1861 ttgcttcgtt gtctaaggct agtttagcca aaaggggatt atctccgaca cctactgtag
     1921 caatcaatcc tagctcttct ttaatattct cctggatcat ttgagccagc ttctttctcc
     1981 gttgacttcg tgttccttca gtcgtaaaaa gattcagtga tcgggtcact tttaagattg
     2041 attcatcgat cgagtaaatc agtagatctt catcagccac atatcttctg aaaatattat
     2101 ttacctgcat atttcgcttg atatataggt tcatacgtgg tggaacaacg tgtagtgttt
     2161 taggaaatgg ttgtggtaag tcacgtggtc tactcacatt tgtaatacca taccgctttt
     2221 ttgcttcagg agaagaagct aatatcaatc ctgaaccagt attatcagct cgactcataa
     2281 caacaagttc tgttgttaat ggatctaaat ttctttctat acattcgaca cttgcgtaaa
     2341 agcttttcat atcgattaaa aagtaatcat ttactggctc ttttgaataa tccatcataa
     2401 ataacactcc attctttcac attatacaaa catatgttcg atattgtaaa gaagaaatag
     2461 ctaaatacat aaaaaaagaa gcataggtat gctatcaccc tgcttcttct tttgatttgt
     2521 atgatcaatt tattctatca ctttactaat taatttgaaa atctaataaa aactctcact
     2581 ctaaaagagt gagagactgc aaattagagt acggactttt ttggagttcc gttctttaat
     2641 attaacaaat gaataaaatc cttacaacat ttgagaataa gttatcagta gacacttaca
     2701 aaaaattctc atactatttc ctccatcatt agtttttgtc tatataaggt ttaccctaaa
     2761 tagacactgg tataacattg aaaaatatgg cttcctcgtg tatgattagg gtgtatttta
     2821 aatagttgca gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc
     2881 gatcaaaatg aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc
     2941 atcgagaaac aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat
     3001 tacgtccgag atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat
     3061 gacgagatca tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact
     3121 agtttaccga tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat
     3181 ctgatcatcc aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc
     3241 caggcacaag gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta
     3301 tacagtgcag acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat
     3361 cttaaaaatg gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc
     3421 gtgtatagaa taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta
     3481 ttatcttctc atctttaatt tcctacatat aattactatc tataaaatta agtcttactc
     3541 agaacagata aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct
     3601 gttaatataa tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct
     3661 atattttcta caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc
     3721 gacacattgg ttatactaat tcctatagct agttcttgag actctacttt tctctctagc
     3781 tcctttgaca aagtgatttg agatttattt ggttccgttg gagtcaattc tatagattct
     3841 tcataaaaat ctctatacat aaa
//
LOCUS       DAZKCY010000176         3851 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000176 DAZKCY010000000
VERSION     DAZKCY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.179"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..828
                     /locus_tag="ACG8XC_002648"
                     /pseudo
     CDS             28..828
                     /locus_tag="ACG8XC_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            1066..1302
                     /locus_tag="ACG8XC_002649"
     CDS             1066..1302
                     /locus_tag="ACG8XC_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192304.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1309..3261
                     /locus_tag="ACG8XC_002650"
     CDS             1309..3261
                     /locus_tag="ACG8XC_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP9192305.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3333..3830)
                     /gene="dfrG"
                     /locus_tag="ACG8XC_002651"
     CDS             complement(3333..3830)
                     /gene="dfrG"
                     /locus_tag="ACG8XC_002651"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP9192306.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1371 a          621 c          712 g         1147 t
ORIGIN      
        1 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
       61 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
      121 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
      181 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
      241 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
      301 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
      361 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
      421 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
      481 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
      541 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
      601 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
      661 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
      721 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      781 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      841 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      901 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      961 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
     1021 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
     1081 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
     1141 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
     1201 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
     1261 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
     1321 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
     1381 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
     1441 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
     1501 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
     1561 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
     1621 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1681 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1741 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1801 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1861 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1921 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1981 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     2041 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     2101 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     2161 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     2221 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     2281 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     2341 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     2401 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     2461 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     2521 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     2581 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     2641 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2701 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2761 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2821 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2881 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2941 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     3001 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     3061 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     3121 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     3181 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     3241 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     3301 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     3361 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     3421 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     3481 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     3541 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     3601 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3661 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3721 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3781 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3841 gcctttttaa c
//
LOCUS       DAZKCY010000177         3713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000177 DAZKCY010000000
VERSION     DAZKCY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.181"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..736
                     /locus_tag="ACG8XC_002652"
     CDS             <1..736
                     /locus_tag="ACG8XC_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192307.1"
                     /translation="TLCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTH
                     SIKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMN
                     YYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLI
                     DLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSD
                     LLIRWQVITNFLMELKKYSNTQTKFS"
     gene            852..1172
                     /locus_tag="ACG8XC_002653"
                     /pseudo
     CDS             852..1172
                     /locus_tag="ACG8XC_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1350..1709
                     /locus_tag="ACG8XC_002654"
                     /pseudo
     CDS             1350..1709
                     /locus_tag="ACG8XC_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            1773..>3713
                     /locus_tag="ACG8XC_002655"
     CDS             1773..>3713
                     /locus_tag="ACG8XC_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9192308.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVK"
BASE COUNT         1374 a          495 c          676 g         1168 t
ORIGIN      
        1 tacgttatgt agtaaatgta atagtttttt tggtagtgag tatgttgagg aatatattag
       61 atttgcagaa gacaataaaa atttccttta taacaataca agtttaaaga atggtaagtc
      121 tgatttaaca cattcaataa agaaaatgaa ttcacttaga gttgcaaaag caattgtagg
      181 aatgtttttt agtttaaatg gggatgaaga ttcaatggat aagccattct tagattcggt
      241 acgagaatat cttagcaatc ctaaaagtac tttatttcct catgaagact acaaaattat
      301 aatgaattat tatcttccaa taggtgctcc acagaattct aaaatcaaaa ttcctaatga
      361 cattgtaaat gatggtgaca ctcacaatgt aataaaaggt tttctgcccc ttgatctatc
      421 aactgggaga aaaattcgat tttcagaaat tcagtatgaa ggaattggtt ttactcttat
      481 agatttaaaa aattcaactt ttgaaataaa agaaggatta gatttaaaaa atctcctttg
      541 tgcagatgat aaaatcaaaa aaatacaatt gtacaaagtc cctatattaa caccaacagt
      601 acttacaatg ttggagatgg aaaataatat taattatatt gactcaggtt cagatttgct
      661 gattaggtgg caggtaataa ctaattttct tatggaatta aaaaaataca gtaatactca
      721 aacgaagttc tcttgaagtt ataaagaata caataaatat aaatttcctt tagactatat
      781 taaaatcagc aatcgaagga aaataaaaga agaaaatggt acgcaaaatc taactattag
      841 agcaaaaaca ggaagctgct ttggaagata gtcccgaatt gtttccgatg ttaaaagatc
      901 gtttaaatga attgactgaa aaacgaagac agttacaaat tcgagagaat gaaatcttag
      961 ggatctttca acaacaaggg gaaccgattc aagtcaaaga tgtccaacag atattgacta
     1021 gtttggattg ctttcttatc cagagtgaga agaagcaagt taaagctttg tatcacacct
     1081 ttattgagaa aatcaccttt gatcctaatc agaagtagat attgccgatc ccgatgagat
     1141 tcacacctac tgtgatttga taattgaatg agtaatatca aatagccgta tccaaaccaa
     1201 aggatatggc tatttttgtg ttagggatat acgtcacgta gcttgttatc aagaataagt
     1261 ttctttattt ttttgctttt tctaaaactg agtatgctaa aataagaggg tatatagcaa
     1321 ctttaaataa gagataggag tgtatctttt tgggtgcaga attaaatcag aagttattta
     1381 gtgcggcaga taatctccgt agtaaaatgg atgcgtcaga atataaaaat tatttattgg
     1441 ggctgatttt ctataaatat ttatccgacc gtttgttgga acaagtggtt cttttagcag
     1501 atgaatcctt agaggaatat gacacagtaa gcaaacaaac gatgttgtat cgtgaattac
     1561 tttcagatga agaatcaaaa gaagatctga ttgcaacaat cgttgatatt ttggggtatg
     1621 cgatttcgcc agagtatctt ttcaacgtct tagcggatca agcaaaacaa gcaacgttcc
     1681 aattgaatga tctaaacaaa gcctttttag caagtcatat ttatggaaaa atactgtcgg
     1741 caaaattcga atagtgtcga ggaggtgctg tgatggaaga attaaaagaa cgcattgtca
     1801 attatgtcat agagaactct gagattaaaa ataaatgggg gaacaataca gcacagaaaa
     1861 tatctgaaaa attaaatgtt agtcgtaata ttgtctctca atatttgaat gaattcgtaa
     1921 aagatggaga atttggaaaa atcaatactc gtccagtaat cttcttttct ttaccggaga
     1981 aagttccaaa tcaacaaatt tttagctctt cagaggaagc ggaaaaatgg tatgatagct
     2041 cttttagggt tggaatagaa agggttatcg gaagtcaagg aagtctgcga aatgtaattg
     2101 ctcaaattaa agcagcaatc tactatccgc caaatggctt acccatttta cttcatggcg
     2161 agactggaac tggaaaaagc tatttagcaa attatgtgta taaggatttg ttggccaata
     2221 attatataga agagaaggca tctttcattt cagttaattg tagtgaatat gccaataatc
     2281 cagaactttt cttgtccaat ttttttgggc atgtaaaagg tgcatataca ggagccgaag
     2341 atggaagaaa gggtttagca gaattagcta ataatggagt cttattttta gatgaaattc
     2401 atgcattgag caatgaatgt caggagaaac tttttcagtt tatggatacg aggaaatttc
     2461 atcggatggg tgataatgaa aactggtaca catcaagttg ctggattata tttgcaacat
     2521 cagaaccacc tgaggataaa ctattatcaa ctttgttgag aagaatacct gtaaagctaa
     2581 cacttccaac attagacagt cgttctcttt atgaaagaaa acagttaatc aagatatttt
     2641 ttaaatcaga agaagaaaga ctagcaaaga aaatactgat atctcaacaa ttattagatt
     2701 ttttattaaa ctatcgcttt agtgaaaatg ttggcaattt gaaaaatatc attcaattga
     2761 cctgtgccaa tgccttttat ttcttagatg atttggaaga actttatatt cagatgtatc
     2821 atttaccaca atgggtgaat ttcaacggca tgaaggttat gaaaagacag aatgaattaa
     2881 aatatattca aatggatgat ttggaaaaag tagacgaagt ttttaatatc aacgaactta
     2941 tagagtatct ttatcaaacg tggaagaaag acactgataa gcagcagctt tttgagaaat
     3001 atgatagata ttttagacaa aagaatgttg atatccaaaa taattcagct ttaacatatc
     3061 ccacctatca agagattcga tttagacagt atgttaagga aatagagaga gattttcaaa
     3121 tagaatttga ctattattat cttcaaataa cgtctatctt tttgaattct tggatttatc
     3181 aaatccaatt aacatcggac gataaattat attccttcat caatgatatt aatagttggt
     3241 gtagtcgaga agttcaatta gtcaatcgtt ttattcaaat aggggatatt tatttaacaa
     3301 aaattcaaaa aaatgttttg gttattttat ttaagttaat gtgtagtaaa gaatttcagc
     3361 aaaaacgttt agcactgatt ttggctcatg gagatctaac ggcaacctgt atagcaagta
     3421 ctgctaatag gatcgttgag agatatatat acgacgccat tgatatgcca ctaatgactt
     3481 caagtagtga gattgcagac aaaattaata gatacttaca agagtgtacc ggatatgaag
     3541 aaattatttt acttgttgat atgggttcac tggagaaaat ttatctaaat ttggaactag
     3601 acgctcaaaa aaaattcgga ctactaaaca atgtttcaac aagaatggct attgatgttg
     3661 caaatcagat attattagat ttaccgctta aacagattct tgaacaagta aaa
//
LOCUS       DAZKCY010000178         3711 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000178 DAZKCY010000000
VERSION     DAZKCY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.182"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..356)
                     /locus_tag="ACG8XC_002656"
     CDS             complement(<1..356)
                     /locus_tag="ACG8XC_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192309.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
     gene            complement(616..1164)
                     /locus_tag="ACG8XC_002657"
     CDS             complement(616..1164)
                     /locus_tag="ACG8XC_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192310.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1165..2022)
                     /locus_tag="ACG8XC_002658"
     CDS             complement(1165..2022)
                     /locus_tag="ACG8XC_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9192311.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2308..2594)
                     /locus_tag="ACG8XC_002659"
                     /pseudo
     CDS             complement(2308..2594)
                     /locus_tag="ACG8XC_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2584..2913)
                     /locus_tag="ACG8XC_002660"
     CDS             complement(2584..2913)
                     /locus_tag="ACG8XC_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9192312.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2990..3262)
                     /locus_tag="ACG8XC_002661"
     CDS             complement(2990..3262)
                     /locus_tag="ACG8XC_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP9192313.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3262..3525)
                     /locus_tag="ACG8XC_002662"
     CDS             complement(3262..3525)
                     /locus_tag="ACG8XC_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9192314.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1086 a          752 c          472 g         1401 t
ORIGIN      
        1 ttaaatgttc ttactgcctc ttccacacta atttcttcgt tcaatttcac taataatact
       61 tgatctcgct tattataatc gctttcattt atcctaagag tcaaatcttt ttccaccata
      121 acttttacac ccaataaatt ttgcggttta tttttctctg ttgaaattct ttgtgtagcc
      181 tttaaactga caattagctc attgggtaca tactctattt gagagtcatc taattggctc
      241 ataatccttt ctttttggtt agtatctgca gttacttgtt tagtatgagc catgaaccca
      301 aacatactca ccccaacaac caatcctaaa tatattttct ttttaaactt tttcatctac
      361 atctcctcct aatagtttta ttttagagtt agctctatta cttatttact aactcaacaa
      421 cagattatca tacacttgtt aatgaaatca caatatctca ttaaaacaca ccctaaaatc
      481 ataaccgttc ctatgctcat tttttggatt ttttaatcac gtaaccagaa aaataaatcc
      541 cctctatttc acgcaaatca aagaagaact aagtaactac cgtttaaagg tagttactta
      601 gttcttcttt gattattatc taactttatt attcattccc caccctttgc gcagtgagtc
      661 ttcggtatct tgttttatct gactggtagt tcttattatt tctccacgat catctaattc
      721 tggaacaatt ggatattttt ctctgaacaa gtctagttct ttcaccacgt cattaaacaa
      781 atcttgctta tcttcttgcg taagattctg tttaatatac tcatctaaag cttttctaag
      841 caattcatct cttgtcatat tagtctcata cttttctact aaaactctgg aagtcgcatt
      901 taacaaagtc gacagtttta aaggatgctt ggtggaataa accttatcaa actttctgat
      961 tggtgcgcca actttttttg ttcttcctct actctgtctt tcaacattgg aggaagtttt
     1021 tatttcttga acttggtgca cttctaattg cttattttca gatggtgtaa ttgcttgctg
     1081 ctttttcttt gcttctgctt ctttttttat tttggcgagt cctttgttgt tcccaaaaat
     1141 atcaagtcct ttgatgtcag ccaattactc cacctccgca taccaattct cgtgttcttc
     1201 caattctttc aaaatactga taaataattc gtggcttttg cgatcccatt gttttaagag
     1261 acctttcttt gtcttctcca tcgtaatacc tgatccgtcg tatcgtttta agcgcgcatt
     1321 tttgagtaca acatttttaa tcaaatgttc cccataaatc tcttgggctt gtttatacat
     1381 ttcctgatct actgcgtcac cgctttccaa catgaaagga ataacaccag caacatataa
     1441 atttgtatca aattctttca caaaaaagtc catatactca agatattgca tcgcatttct
     1501 catcgatttt acttgggtct gtaagacgac gattgcccaa tccgcaatgt acatcgcgga
     1561 tttactatat acagaaatag aaggtgggac atctaaatag atgacgtcaa actcatcaaa
     1621 taatggttct agcttttctt tcatatattt gaattgggat aatgaatcat tatattggtc
     1681 tttgatccaa tcatcataca tttcaaagga catgtctgct ggaagtatat agaggttatt
     1741 gattacttta tagacagcct ctttaggtgg aagattgttt atcaacacgt cagtcacagt
     1801 attttcgtaa acaatcggtt caccttctcc gcctccttct tcgttagtga agttcatatg
     1861 cgttaagttc atcacatcag aagtatttcc ttgcatatcc aagtcaatta tcaaggttct
     1921 ttttccttca ttaactgctg attcaaaccc taacatttgc gcgcacgtac ttttacctac
     1981 gccaccttta aaattaccga ttacatattt cttcgctacc acgttcctac ctcttttctt
     2041 ttagcaatat tcatttgata ctattataac atatttattt ttagttacta ccaaaaaaaa
     2101 gatatatatg tactacctta taattaataa gtaaaaggta gtacataaat aaggaaaagc
     2161 tgaaatatat tgatttatag ctttcctcaa agtaatgacg ttatttaatt tatcgagaag
     2221 cagagaatac tcgcgacttt agtcatgtag acgaatcaga ttttcgataa aaaagaagcc
     2281 gtccttctta agaacgacaa caaatcacta gattaatttt ttcaactgtt cataaaagtt
     2341 ttcatgcgtt ccacataaca atactggaat aaccttgttt tctataatct cgtaagctac
     2401 tctatatgtt gtgcccgcat atttgaaccc actagaccaa atacctgata aatccccagt
     2461 tttttgttca cccttatatg gatcaatcgc aatttcatca taaattaaat tcaaaaattt
     2521 ttctttcaat ggcttctctt ttagcttttt aaatacttaa tgacatgttt tgaaggaacg
     2581 atttcataag ccaatttctg ttgccaactc ctctttagtc attactttag aattagtaag
     2641 tgtatcttca gcaatatctc taaaggaaga atgcattttg ttttttctat tcttaaattc
     2701 tacaagaatc tcatctttat cgtagccttc tgcaattata tcagacaaaa tatcttcgct
     2761 aaaatcataa aattcttttt gtggttcaac aaattcataa agaataccat tctctaccaa
     2821 ctttgcttca acttcgactc cattaggaac attaaaatct ttaggtactg tcaacatgat
     2881 tgaattgcct tgttttcttg tttttgtaat catttaaatc cctcatttct aacagtatgt
     2941 aattacttag taaatacatt ataccacgag ataatgtatt tacaagtaat tactgtgact
     3001 cgtaatgcgt ccttgctgaa taaatttcaa ccacgtgttc atcttcatct accttataaa
     3061 caacccggtg ttgtctattc aatcttctag aatacctacc ttcatgtgta ggttttaact
     3121 tctcaaatga ttgggtaggg agatagggat cttcctttaa agtctgaata accttctcaa
     3181 attgaggttt caaattagac tgtttgattt tctttaaatc agctttagcc gagtttttaa
     3241 tcatcacaga atagttattc attataagtt atcccaatca atttcatcaa cattagtaaa
     3301 tccactatca tctttttctc gcttacgaac aacacccaaa gtgcctattt gctcaagtaa
     3361 tagcgtttct ttgattgctt tccaatcatc caaaccaata agcaccgcat tattttcact
     3421 gcgctcacta atgatttcaa tttccgtatg attctcattt acatctttca acaacttata
     3481 aaaatttttc cgtgctgagc taggatttaa gactgttttt gccatatcta ctccccctct
     3541 aatttatatc tccattctaa cgtacttaat taagtacgta aattcgattg ttttgtttaa
     3601 attggagtac caaaacagca ttgtaaagtg ttatccgttt cctttatacc cgtggtgcta
     3661 gttaattttt tcctagagga aaatgtctaa acatgttggt aaatcaacaa a
//
LOCUS       DAZKCY010000179         3693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000179 DAZKCY010000000
VERSION     DAZKCY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.183"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            257..388
                     /locus_tag="ACG8XC_002663"
                     /pseudo
     CDS             257..388
                     /locus_tag="ACG8XC_002663"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            464..957
                     /locus_tag="ACG8XC_002664"
                     /pseudo
     CDS             464..957
                     /locus_tag="ACG8XC_002664"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1127..2023
                     /locus_tag="ACG8XC_002665"
     CDS             1127..2023
                     /locus_tag="ACG8XC_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9192315.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            2122..2331
                     /locus_tag="ACG8XC_002666"
     CDS             2122..2331
                     /locus_tag="ACG8XC_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFP9192316.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            2348..2620
                     /locus_tag="ACG8XC_002667"
     CDS             2348..2620
                     /locus_tag="ACG8XC_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9192317.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            2622..3485
                     /locus_tag="ACG8XC_002668"
     CDS             2622..3485
                     /locus_tag="ACG8XC_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP9192318.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
BASE COUNT         1392 a          567 c          697 g         1037 t
ORIGIN      
        1 aaaaagcttt aactacaagc tttttagaca tctaatcttt tctgaagtac atccgcaact
       61 gtccatactc tgatgtttta tatcttttct aaaagttcgc tagatagagt tctatattag
      121 agatgtaaag agttttggtg aagaggtttg tgagtatggc ttttataacc agggtgcaat
      181 gagaagcact aacagcagct agcttaagaa cgcataggat atacttttta tggaagtcac
      241 agaggaggaa taaaatttga acaggattaa cagagtaacc tctattctta ttcagctgca
      301 atcaaaaaaa ataattcctg ccaaagaaat tgcacagcga tttaatataa gcttaaggac
      361 agtttacaga gatatccgga cacttgaaga agccggaata ccaattggat ctgaggccgg
      421 aaaaggatat tttcttgtag agggcttcct acttccgccg gtaatgttta cagcggcgga
      481 agtgggtgca ttgattacag cagggaaatt tttaaattgc catggagatg aatcatttat
      541 aaaggatttt gattcagcta tgtataaaat caaatctatt ttgaagcatg gtgaaaaaaa
      601 ctatgcacag gagttggaga acagtattaa cgtgtacagc acatctggac agaaaaatac
      661 attggcggat aacgttattg cagcaattca gactgcaatc tgcaataaaa gggtaatatc
      721 aattcaatat cctgcatcag gagggcaaga accggaaagc agaatgatag agcctatatc
      781 actggggttt tatgaacaga actggtactt aatcggtttt gcaggctaag aaatgaatat
      841 aggaattttc gtgtggatcg gattaaaagc atttgcatag aagaagagct ctgtcaaagc
      901 catgatggct ctttggaaca aatattaaaa caaatgcttt catataaaaa actatatgcc
      961 ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt gattttatat ctttctcaaa
     1021 ataaatataa aagcccttta aagagggctt ttatatatta atcacaaatc acttatcaca
     1081 aatcacaagt gattaatcac aaatcacttg tgatttgtga ttcttaatga tacaatatta
     1141 ctatacaaaa aaagaatggg gcgtagttat ggagaaggaa aaactaaaaa tacttgaaga
     1201 attaagacgt attttaaaca ataaaaatga agcaattatt atcttgaata attactttaa
     1261 aggtggtgtt ggaaagtcca aattatcgac tatgtttgct tacttgacag acaaattgaa
     1321 tttaaaagtt ttaatgatcg ataaggactt acaggcaaca ttgacaaaag acttagcaaa
     1381 aacttttgag gtagaattgc cacgtgtcaa tttttatgaa ggcttgaaaa atggaaactt
     1441 ggcttcttct attattcatt tgactgataa tttagacttg atccctggca cgtttgattt
     1501 gatgttactg ccaaaattaa ctcgctcatg gacgtttgaa aatgaaagta gattgcttgc
     1561 tactctttta gcacctttaa aaagtgacta tgatcttatt attattgata ctgtaccaac
     1621 gccaagcgtt tatacaaata atgcaatcgt ggcgagtgat tacgttatga tccctttaca
     1681 agcagaagaa gaaagtacaa acaacattca aaactatatt tcctatttga ttgatttgca
     1741 agaacagttt aaccctggac tagatatgat tggttttgtt ccttatttag ttgatacgga
     1801 cagcgcaacg ataaaatcaa acctggaaga actgtacaaa gaacataaag aggataattt
     1861 ggttttccga aatattatca agcgaagtaa taaagtaagt acttggtcta aaaatggcat
     1921 tacagaacac aaaggctatg acaaaaaagt tttgtctatg tatgagaacg tattttttga
     1981 aatgattgag cgaatcattc aattagaaaa tgaaaaagaa tagaatcaca aatcacaagt
     2041 gattaatcac aaatcacttg tgatttgtga taggtgatga taaaataaat agtaagaaga
     2101 aatagaaaga agtgagcgat cgtgggaaat ttaggcgcac agaaagcaaa acgaaatgat
     2161 acgccaatca gcgcaaaaaa agatataatg ggggataaga cggttcgtgt tcgtgccgac
     2221 ttgcaccata tcataaaaat cgaaacagca aagaatggcg gaaacgtaaa agaagttatg
     2281 gatcaagcct tagaagaata tatacggaaa tatttacctg acaaacttta aaaggagtga
     2341 aaatgaaatg gcagttacgt atgaaaaaac atttgaaatt gaaatcatta atgagttatc
     2401 ggcaagtgtt tataatcggg tattaaatta tgttttgaat catgaactag atactaaaaa
     2461 tactcgttta ctagaagtga atcttttaaa tcaattagaa gtggcacaag aagttgattt
     2521 atttcaacaa ccatttgaag aattacaagc tattcatgag tattggcggt caatgaatca
     2581 atattcaaaa caaattttga ataaagagaa agtggcttaa catggcgaat attactgact
     2641 tcaccgaaaa gcaatttgaa gatcgtttag aaaagaatgt tgaacgacta actaaaaata
     2701 gactagcggt tgaatcgcca accgcttttt tacttggtgg gcaaccaggg tcagggaaaa
     2761 ccagtttgcg atcagcaatt tttgaagaaa cacaagggaa tgttattgtc attgataatg
     2821 atacctttaa acaacagcac ccaaattttg acgaattagt gaaactctat gaaaaagacg
     2881 tagtaaaaca cgttacccct tattcgaatc gcatgacaga agcacttatt agccgtttaa
     2941 gcgatcaagg ctataattta gtgattgaag gaacaggacg aacaacagac gttcccattc
     3001 aaacagccac aatgcttcaa tctaaaggtt atgaaacaaa aatatacgtc atggcagtac
     3061 cgaaaattga atcatactta ggaacgattg aacgatatga aaccatgtat gcagatgatc
     3121 caatgacagc tagggcaaca ccaaaacaag cgcatgatat tgttgtcaaa aacttaccga
     3181 ccaatttaga aacccttcat aaaacgggct tatttagcga tataaggctt tacaatagag
     3241 aaggagtgaa actctattca agcttagaaa cgccttccat tagtccgaaa gagaccttag
     3301 aaagagaatt aaatcgtaaa gtatcaggga aagaaattca accgactttg gagagaatag
     3361 agcaaaaaat ggttcaaaat caacaccaag aaacccctga atttaaagca attcaacaaa
     3421 aaatggaaag cttgcagcta cctacaccac caatacccaa aacacctaaa cttccaggta
     3481 tttaaacctt aaaaaaggaa aagagtagtt accaaaaaac ggtaactact ctttttttat
     3541 aaaaacattt cctcatgttg gggtcccgag cgcctacgag gaatttgtat cgataagaaa
     3601 tagatttaaa aatttcgctg tttttttcta atttcatttt gaggtgaatt aattggtagt
     3661 tattagaggt gccctatacc taataattta tct
//
LOCUS       DAZKCY010000180         3626 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000180 DAZKCY010000000
VERSION     DAZKCY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.184"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..701
                     /locus_tag="ACG8XC_002669"
     CDS             <1..701
                     /locus_tag="ACG8XC_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9192319.1"
                     /translation="TKNTILYNVAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLL
                     PYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGI
                     GYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFR
                     ADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMIT
                     NTIARRFDSDSALF"
     gene            713..1633
                     /locus_tag="ACG8XC_002670"
     CDS             713..1633
                     /locus_tag="ACG8XC_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9192320.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1674..3131
                     /locus_tag="ACG8XC_002671"
     CDS             1674..3131
                     /locus_tag="ACG8XC_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9192321.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3476..>3626
                     /locus_tag="ACG8XC_002672"
     CDS             3476..>3626
                     /locus_tag="ACG8XC_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9192322.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLY"
BASE COUNT         1159 a          629 c          736 g         1102 t
ORIGIN      
        1 tcactaaaaa tacaatttta tataatgtag catttatcct tttgaatctg ctgatctcag
       61 tcgtatttgc aatcattatg agcgagttga gaaataaacg actcgttaaa gtttatcaaa
      121 cgatgtcctt actaccttat tttctatctt gggtcattat cagttacttt gtttacgctt
      181 ttttaagtcc tgacaaagga atattcaatc agtggatcac tggacatgga ggagaagctg
      241 tcaattggta taatgaaccg aaatattggc cgtttatctt ggtgttcatt ggtacatgga
      301 aagggattgg ttataacagc attatttact ttgcatctgt tatgggaatc gatccgactt
      361 attatgaagc tgctatggtt gatggagcta gcaaatggca gcagatcaaa catgtgacga
      421 taccacaact tattccacta atgacgattt taacgatttt agccgtagga aacattttcc
      481 gagctgattt tggcctgttt tataacattc ctaggaattc cggtgcatta tatgatgtga
      541 ctcaagtttt ggatacgtat atctacaatg gcctgacatc cacaggagat tttggtatga
      601 cggctgctgc aggactttac caatcagttg tcggctttgt cttgttgatg atcacaaata
      661 cgatcgcccg acgttttgat agcgattctg cattattcta aggaggaaaa aaatgaaaaa
      721 aaagaaagtg gagagagtcc aagtccgctc tttcaataag acggctaatt ttttctctaa
      781 tataatgatt gcactctttg ctatttcctg tattttgcca tttatatttg ttatcgccat
      841 ctcttttacc gatgagtcca tattgtcgac tcacgggtac agtttttggc cagaaaagtt
      901 cagtatattt ggttatacgt acttgttcga ccagatgcag gataaaattt tccaagcgct
      961 tttcgtcact gtacttgtta ctgttttagg tacactgatc aatagcacgg ctacttcttt
     1021 atatgcttat gcgatttcac ggtcaaactt tccattccgt cgttttttta ccgttttttg
     1081 tttgattacg atgttgttct caccagggat ggtggccaat tatcttgtta tgacaaatct
     1141 tcttcagtta aaagatacga tttgggcatt gatcttgcca atggctgtca gtcccttcaa
     1201 tattattgtg atgcgcacat tctttaaacg ttcggtctct gattcgatta ttgaatcggc
     1261 acggatcgat ggagctagcg aactgcgaat atttgttcag atcgttttac cgttagctgt
     1321 tccaggtatt gcgacaatca gtttatttgc cgcattgggt tactggaatg actggttcaa
     1381 tgctttacta tacatccaag atgataagct agttccacta cagtatcttt tgatgaaaat
     1441 ccaaagtaat attcaatatc tgacacaaaa ttcaggagca ggaagccaat tgacaggcgg
     1501 attagcctca gttccaggag aatctgccag aatggcgatc gtagtcatct cgacgttgcc
     1561 tattgcgatc agctatccat ttttccagaa gcattttgtc aaaggtctaa caatcggcgg
     1621 agtaaaagaa taaagaaaca aaaatataaa tcataaaatt aggaggaaga aaaatgaagt
     1681 tatggaagaa actagccttt acaggtgttt cagcgttgtt gttgggaaca ttagcagcat
     1741 gcggaggttc tggttctaaa gaccaggcag aagctagcaa ttcggacact ttgcagatgt
     1801 atcaaatcgg agataaacca gataatttcg atcaattgat ggaagtagca aacaaacgaa
     1861 tcgaaaaaga gatcggtgta aaagtcaata tcaattacat tggctggggc gattacgaaa
     1921 agaaaatgaa cgtcatcatt tcttccgggg aaaattatga tatcgccttt gcgaacaact
     1981 atgtttccaa tgcccaaaaa ggagcctttg ctgatttgac tgaattagcg cctaagtacg
     2041 ctgaaaaagc ttataatgat ttagatgaag catatattaa aggaaatctg gtcaatggaa
     2101 aattatatgc tttcccagtc aatggaaatg tctttgctca acaagtactt acttttaaca
     2161 aagctttact agataaatac gatctttcaa ttgatggaat ccaatcttac gctgatgcag
     2221 aaaaagtctt gaaggaattc catgaaaaag aaccaaacac ggcagctttt gccattggac
     2281 aaggcttcaa agtccaaggg aactttgatt atcctttagg gaatacgtta ccatttgcta
     2341 ttgatctgga tggagatgat tcgaaaatca tcaaccaata cgataatgaa acgtatatcg
     2401 gactgttacg tacaatgcat agctggtaca aacaaggact cattccaagt gacgctgcaa
     2461 cgagcaataa ggattatcca ctagaaggaa atacttggct aatgcgtgga gaaacacaag
     2521 gaccgtatga ttatggcgat acaattttga caaatgcagc aaaacaagaa cttgtctcaa
     2581 aagcaattac tgttccacta aaatctacag ctcaagcaca aatggcaaat tttgtcgtat
     2641 caaatacttc aaaaaataaa gaaaaatcag tagaacttct agggctgctt aacagtgatc
     2701 cagaattatt gaatgggctt gtttggggga tcgagggaga agcatgggaa aaagtcgatg
     2761 gttcagatga taaaatcaaa ttgctagatg gttatcagcc aaatacacat atgtctgctt
     2821 ggaatacagg aaacaacaag attctatata cacaagagtc tattacggat gacatgattg
     2881 ccaaacgtga tcaatcgatt gctgatgcag aaacttcacc gattttagga ttcagcttca
     2941 acactgacag tgtcaaaaca gaattaagta atatttctaa tgtcatgaat caatatttag
     3001 atggattgaa tacagggact gttgatccag atgaaacatt accgaaattg aaagacgctt
     3061 tggacaaggc tggctatgat aaggtattga aagaaatgca aaaacaatat gatgagttcc
     3121 gacaagaata ataatgaata aagacaaagg ggttgtgaca aaagacgtgt cacaacctct
     3181 tgttccgaat aaacggtgtt caaaagccag tttctcggta ttaagctaaa tatatgtaaa
     3241 aatgtggaaa gccatattca caaaattttt cttactactc gaaactaagc ggctatttca
     3301 caaactcttg gtaaacggtg ttcaatcagc tgaccctcgg tattcgctca caaaacgaca
     3361 aaatatgagg aactattttt ccgtttgttt ctctaataat caggtcaagg tgctgattgc
     3421 ccaacctttt tttgtataat gagaaagtat aaacttaatg tttggaggag aagatatgtt
     3481 agggaaggtt ttggaagagt tatttatccg gatctgggtc attataaaac taaccttgta
     3541 tttctggata tatacatttg ccggcgggat catctttgga ttaggtgcgg catggaaaac
     3601 agtcaatgaa ctattttatt tgtacg
//
LOCUS       DAZKCY010000181         3479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000181 DAZKCY010000000
VERSION     DAZKCY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.185"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..203
                     /locus_tag="ACG8XC_002673"
     CDS             <1..203
                     /locus_tag="ACG8XC_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9192323.1"
                     /translation="PLNENSNGILRRNGLPKSMDFREVNQTFISSVSNQRNHIPRKSL
                     NYRTPIEIFLSYVQEAFYSSLI"
     gene            697..984
                     /locus_tag="ACG8XC_002674"
     CDS             697..984
                     /locus_tag="ACG8XC_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192324.1"
                     /translation="MEIKEIKVRGINKKYVQEIDHRCVELTEQTGQKWSRNDYLKLLI
                     ENDFERPLMDYKKDQFDRLLEKFTDVQLHNTKVLEAYTNEVKNLIEILIAH"
     gene            1002..1424
                     /locus_tag="ACG8XC_002675"
     CDS             1002..1424
                     /locus_tag="ACG8XC_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192325.1"
                     /translation="MVKAIEKHITFYEAHDEVLKHIMETQPTVKNYSEAIRFLCMNYD
                     PEIEKNNNMTNAKLNAMGKEISILIEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            1444..3036
                     /gene="mobP2"
                     /locus_tag="ACG8XC_002676"
     CDS             1444..3036
                     /gene="mobP2"
                     /locus_tag="ACG8XC_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFP9192326.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQNSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQIEQERQ"
     gene            3292..>3479
                     /locus_tag="ACG8XC_002677"
     CDS             3292..>3479
                     /locus_tag="ACG8XC_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192327.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1386 a          537 c          664 g          892 t
ORIGIN      
        1 gcccattaaa cgagaattct aacgggattc tgcgtcgtaa tggactgccg aaatcaatgg
       61 attttagaga agtgaatcag acatttattt ccagtgtcag caatcaacgt aatcatattc
      121 caagaaaatc attgaattac agaacaccaa tcgaaatatt tttgagctat gtacaagaag
      181 cattttattc tagcttaatt tgacaaatca tatgaaaaaa gtatataaaa aatgtttatg
      241 gatcagctat atcaccctag gtatagctgt taaagagatg ggagaaaaca ttaaccacga
      301 ttctaacaca aacatcaagc acataaaaca tttaacactc ctcctttctg gctgaagatt
      361 tcaaccaaac aggaagacag cgtatcaatt atagcataca ctttttctta aattcatttt
      421 tcatatgaca aaatatttcc ttctaatcga caaagttaga aggatttttt attgtctaaa
      481 tacacaaata accttaattt ttaaagagca taaggaaacg tgggaacact ttttaaaaaa
      541 agtgttccaa taggctgtgc ctattacgtt tctctaaaac aaaagtgggt acaaaatatt
      601 cgatttatgg atagtgtatg gatagtaaaa cagctataca tggatagcat atggatagac
      661 tccatagact atccatactt ttttcggagg gtgaaaatgg aaattaaaga gattaaagta
      721 agaggtatta ataaaaaata tgttcaagaa attgatcata gatgcgtaga attaacagaa
      781 caaactggtc aaaaatggag tcgaaatgat tatctaaaac tattgattga aaacgacttt
      841 gaacgtcctt taatggacta caagaaagat caatttgatc gtttattgga aaaatttaca
      901 gatgttcagc tgcataatac aaaagttttg gaagcataca cgaacgaggt caagaactta
      961 attgaaatat taatcgcaca ttaggaagga gataatgttc tatggttaaa gcgatagaaa
     1021 agcatattac attttatgaa gcgcacgatg aagttttaaa acatattatg gaaactcagc
     1081 ctacggtaaa aaactattct gaagccattc gatttttatg tatgaattac gatccagaaa
     1141 ttgaaaaaaa taataatatg acgaacgcaa agttgaacgc aatgggaaag gaaatttcta
     1201 ttttaataga aatggtttca gagtttgcgg atcttcattt gaaagataca ggaatattaa
     1261 ttggtacaaa ttcaactgtc tatcaagaag caaaagagcg tgtggaaggg aaaataaaag
     1321 cgaatcaaac gaagaagtat tctcctaaaa tcaagaaatc aataaatgaa tctgttcatg
     1381 ccagagtaga cccgtttgat ccaacaaagt ttttaaaaag ataaaaagta gaaggggaat
     1441 atgatgaata aatcgcctgc agtcattctc acttgtcaat ttaccttgcc caataaaaaa
     1501 tctttttcaa catacattga ttatatgaca agagaaaaag cgctggaaga aatggaacga
     1561 cgtacgcctg aagaagaaca ggaactgcaa cgaatcaaga atgctttaga ggatttttat
     1621 attcctgaag gagaaacttt ttcggagagt aagacggata aagaattcag cgaaacgtcg
     1681 aatgaagctc aatctttgat gggaaatgga atcaactttg acaagttaga ggaacaagat
     1741 tttaccaaat atctttctta tatgacccgt cactatgcgt tagaacaaaa gaaagaactg
     1801 acgcaaagtg aacaaaaaga ataccaacgc ttgaaccaag caataaaaaa atatcaaaca
     1861 gagccacaca aaaaagcttc aggaaacctt cctggagttt tttctatggc ttcagacgaa
     1921 gtgaaaggaa gtgatctgaa ggagattaag caaatcttcc aaagaggaca acaaaaaggc
     1981 tcaatcattt atcaagatgt tgtctctcat gataattcgt atcttgaaaa aataggattg
     2041 tacgatccca aaacagatac tttagatgaa gaaggattaa aagcagctgg aagaaaaatg
     2101 atggagacat tattcgagaa agagcaactg aacgaaacag gctactggat ggcaacgatt
     2161 caccgtaata ccaaacatat ccatattcat tttgcagttg ttgaaaaaga aaatacgcgt
     2221 aaaccttaca cggaggttga aaatggtgtg agttatatcg taccgaaagg taagagaaga
     2281 caagcaacat tagacgcaat gaagaccaat tatgtacatg agttagaacg tttttcttat
     2341 gaaaaggatc gaatgattgg tatggagaaa cgaaatttat tgacgcggaa aagtgatttg
     2401 cgtaacacct taacaaaagt ggtaaaagag ccagcgcgtt acgatcgaca cgcaatgaac
     2461 ttattaaatg aagtatataa aaccctacct gacaaacgtt cggaatggca ttatggggat
     2521 gaaaaacgaa ccaaactttc accatacaca agagagaaac tcgatgagtt aaccaaatat
     2581 gttttaacaa aagaacctga atatcaagaa tatatacaga tttcaaaagc attgaaagaa
     2641 gaagcaaaaa gtctttatgg ggaaagcaaa agagaatcaa aggatgcaga aaaaaatgct
     2701 ctctttgatt tgaaaaagcg cacaggaaat gccattctaa cagaactgaa gaaacagaat
     2761 agtcaaataa aacctttaat agaaagtatg gatcagatgc aatctattta caatgaacga
     2821 tcctttcgaa cgcttaaaca gttaccaacg aatgaataca aatatagtta cgaagaattc
     2881 atgaagcgca ggaaacaatt ccaacaaaga gttgtcacgc aacggagttt gcgtcaatta
     2941 aatcgaacga ttaatgccca tgaagaaaaa taccacgcaa gtaaagccta cgaagagcaa
     3001 caacgaagaa tccaaataga acaagaaaga cagtaaagac agtaaagtta gcgccttatt
     3061 atactacttg ggaaatgaat gaagagggga tcaataactt cccgtgtaaa agcattgaag
     3121 agtctatttc actcacagaa tgttagttgt taagtgaata aaagaagtaa aagagtcctg
     3181 aacgtaattg tccaggactc tttttttcaa tgaattgttt tccgccaatt caatgagtgg
     3241 tgcgatttgc gactacatta tatcaaaaga aagagaaggg aggaagaaga aatggcttat
     3301 tcaacgattg gagttgttgt tgaaaaaagc agagacaatc tcgtttttgt gacagaaatt
     3361 caaacaggca gagcttttgt cgtcacagac aaagcggcaa aagcgtatca aaatggcgat
     3421 attttaacgt taaatctgac tacgaagacg ttcgtcgatg cggcagaaga ttatccatt
//
LOCUS       DAZKCY010000182         3462 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000182 DAZKCY010000000
VERSION     DAZKCY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.186"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="ACG8XC_002678"
     CDS             complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="ACG8XC_002678"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP9192328.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="ACG8XC_002679"
     CDS             complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="ACG8XC_002679"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9192329.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="ACG8XC_002680"
     CDS             complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="ACG8XC_002680"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9192330.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2590..3312)
                     /gene="tsaB"
                     /locus_tag="ACG8XC_002681"
     CDS             complement(2590..3312)
                     /gene="tsaB"
                     /locus_tag="ACG8XC_002681"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP9192331.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT          991 a          757 c          594 g         1120 t
ORIGIN      
        1 aaatatcagg gtcactgctt attttcctcc ctaaaacttc tgaaagatcc ggcagtttta
       61 gatcactgga caagactcaa agattttcta tgtgtgtttc agttatgtaa tattcactgt
      121 ttgcaaaaaa tactgttaga ttaactagat gaagcgtctt gtcctcatcc gatctagatt
      181 attcgatatt cctcgacaat aaacgattac tcagtgaggg caatcagagg aacggataat
      241 gataaataag ctgtaaaaaa tagcaaaata acaataatga ttaaaagtat gtaaaaaata
      301 gggctttgac aaaaacgtgt cacagcccta tttcgattaa acggtgtgta cctgtcaact
      361 cttcggtcac aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt
      421 gtgaatcaga gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg
      481 catacgcggt aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt
      541 tcttattact tgatgcaggg cagctttttc acaacctctt tattcagata aaaccaagct
      601 tggatcagca ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc
      661 tgctccaatc attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac
      721 ctcagggagt ttagttgcca aagcatcctg caatccttca cgcaaccctt gattggccgc
      781 tacacctcca gctataatca gttgcttgac tggataattt tgacaagctc gaagtgtttt
      841 agtaatcaat acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag
      901 atcttctcca cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct
      961 aaaactgaaa tcataattat cttcatggat catcgctcgc ggaaaatggt aattatcttt
     1021 tccctgatga gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct
     1081 tccaactttg tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata
     1141 tgatccatct tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat
     1201 caatggaaac tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt
     1261 tacaggaata agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat
     1321 cagcaacgag ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc
     1381 cgacacacca gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg
     1441 gcggctagct acttcaggta ctacgccacc aaaacgttta tgactgttta tttgagaagc
     1501 aacgatgtta gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca
     1561 acttgattca atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt
     1621 ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat
     1681 atacgaaact tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct
     1741 aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt
     1801 gcatgccctt ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct
     1861 attactttcg agtatccaat aaagccacag atctggttat cttcagctaa aagcagataa
     1921 tcagagtgag gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt
     1981 ggataagcat tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct
     2041 ttttttctgc aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca
     2101 tcttcttttg tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa ccccaatttc
     2161 cgataaagtc tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag
     2221 cgattcatta tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc
     2281 tgatatgccg gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa
     2341 ccgataaaac caatcattct tccattatca aagataccta gatatagatg aggaagcggt
     2401 gacttcaatt ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct
     2461 cgttcgacat ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga
     2521 cgatcgattt gttcgactgt tttttctgta taaatcattt tatcttgaaa aaagcttctg
     2581 gcatacgctc taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca
     2641 tttttcttct gcttctactc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac
     2701 tggttcaact aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg
     2761 aactgtatta atcatcgcac taggcaatat ttcctttatt tcagagaaga atttgtgaca
     2821 atcactcccg acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga
     2881 agcagaaata tgtacatctt caagtacagt ttctaatcta ttttctttcc agcggtatgc
     2941 cccagcgtat acattttttc ttcgagcatc aaacatgggc acaataatat tatccacacc
     3001 gatgcaatta gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt
     3061 ataagcaatc gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc
     3121 atcagaaacc gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg
     3181 gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc
     3241 aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc
     3301 taatgtaatc atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata
     3361 cttttttatt ctgaggatga aaataccaaa ggcaaaacta gtacaataat acatgattat
     3421 ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa aa
//
LOCUS       DAZKCY010000183         3448 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000183 DAZKCY010000000
VERSION     DAZKCY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.187"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            163..358
                     /locus_tag="ACG8XC_002682"
                     /pseudo
     CDS             163..358
                     /locus_tag="ACG8XC_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(1890..2408)
                     /locus_tag="ACG8XC_002683"
     CDS             complement(1890..2408)
                     /locus_tag="ACG8XC_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFP9192332.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2775..3023
                     /locus_tag="ACG8XC_002684"
     CDS             2775..3023
                     /locus_tag="ACG8XC_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192333.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1179 a          589 c          501 g         1179 t
ORIGIN      
        1 aggcaatctt aattttttaa attgtctaaa ttatatagaa agaacaatct ttctatataa
       61 ttagacataa gagagactac ggttgatttg atattaacaa gcgtatatga atgtatatac
      121 aaataaaaaa ggggagggag aagttaaaga aaattcagag aagtggggca ctcagtagta
      181 ttaacaattt aaaaggaatt tcttaaagaa aatgggttgc atcctggaga tacaattttt
      241 ttagataaag aaaaaattca agcaacaatt caaaaaaatc ttaactgctg agattgatgt
      301 agcaatatct caatcatcaa caaagtacga tcctgccttt caaaagctta ttgataaatg
      361 tttattcatt ataaaaataa aagaaaaatt tatgtcagct tttttcatca aagtggtaat
      421 gataattcgt ctattaattg tattttttag gaaatatggt ataattgttt taacgaaaag
      481 gtaattcatg atacaccaaa aaccactagc tggcgggcta gtggttttct tttacgttct
      541 attcagatta acgggtaggc ttactgtctt ttttggcaat aattttgcaa tcaagccatt
      601 tacagatgta atgcgcggat acttctgcta agattgcaaa aaacatatcg aaaaggtaat
      661 tcataataca acctcctccc cttgccataa taggccgggg tagcagacag cataaatatt
      721 ataccataac tgaaaatttc attttagtac atttcaatat attgatgaac cagcacaata
      781 aaacatgcca tgactaacag aaaaaaactt attaaaccag atataacttg taaacatcca
      841 actatataga aaacgtaaat tttctatata gttggattaa gataacgact atttatttta
      901 tcatattttt actataccct atacttttac ttggtatatt tatgaataat cgcaaattta
      961 cccctatact actagtttgt aagttagatt aataaaaata ctaaagttat ttgttctttt
     1021 attaaataca cctacaactt ataaattgat ggtatttaga taacacgaaa gaataaaaat
     1081 aacagctgat agtaaagttt gttgacgatt gctactcaat cgaaagtgag ttagtgcgta
     1141 tgaacttgct tctgaaaact tatgaaatga aagacctttt acatcttgca gaaatcgtta
     1201 ctgggattaa actaagcaat aaaaactaat gttcccctca ttctttgaac ttctactatt
     1261 tgatttattt attctgataa atgacgattt caccttcagg atcttcaaat cccgtataga
     1321 atttatttaa gatctcttca gtatcagtaa taggataggc gataggccac ataccctttg
     1381 atggttagaa taaactgctc aaaatctgta tcgtatgttg gctttagcat attaacaaag
     1441 gctatctcca aattataaac cataagcgtt gaatcgttct taacaatttc aattcttcct
     1501 aacccaaagt cagaagatat aattaccgga cttgccttac ccttcatgtg ggttgttgag
     1561 attccatatt taaagggaat tgtcggtact atcggcggtc tgaaaaacat cccaaaaata
     1621 aaaaaatctt tcccctgcta gtctcctaag tccctttgta gcggatatgg caccataatc
     1681 tcattttatc gaagtgagtc atggtttatc tgttggccaa ccaaaattga tacaatctaa
     1741 cgatgcaccc tctacggcat tctaggggag tgcacttgca ttcacccaat aaaaaccggc
     1801 agttagcaaa gaacttatgt agtcgtatgt tcttactgtt aacatacaag aggtctcctt
     1861 tccacaattg cttagcagta aacgggaatt tacaaagcta atctcttcca ttttcttatc
     1921 tttgttctaa aagtcccaaa tggagcaact gtatttacat gaataaattt atatacttcc
     1981 catacagccg ttttggttgc ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct
     2041 tcatcactca ttgaatcaat catcatgtta atatcatcaa tattacgatt gagaatttcc
     2101 tctaattctt tcaatggtag atgtgcatag gtatttgtaa accacttata taactctcct
     2161 aactgattcc atttaaattg atcagaaggc gttttaacag ataatccatt tctttcatca
     2221 ttttcccatt ttagaattaa attcgtccaa ccaacttgat atgctaaatt ttcagcaggt
     2281 gttctatcca cttcctcaac tcttttatcc ttcaattctt caggaatagt ttcaaattct
     2341 aaaatatatt tagtataagt cctatttatt tcttttttta attcttcttt atttttatat
     2401 gttctcatta tattacctcc ataaattcct aattgtctcc tttaattcat ctataatttc
     2461 atccacgata ttttaatccg ttctacaatg catatctgat atgcattgac tgtctaactc
     2521 atatgtgaat gatttataaa atatcccaac gtctcatcta aagaaatgtt gatcgtcagc
     2581 aaggagctaa aaaatacgaa tatatttctt ttgtagtaaa atcggactgg tttgcatatc
     2641 attttcttcg taaatatcaa aatcatgtta actactatca attactaata aaaactacca
     2701 ctttaagcgt taagaactgt taagtttagt taagtgagga tagttcacaa aatccattgt
     2761 taaacttcac gaaagtggta aaactcaaaa tgctttatat aggattacag catcgctctt
     2821 tcttcgattg cgcgttgggt aaaaaatatt atgaagtaaa aattgacgat gacacgttcc
     2881 ttacggctca tcagatgaaa cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa
     2941 ttctaaaaaa gcgattgcca tattcacgcc tcgctcgacg aactattaaa ggtcaagatt
     3001 tacctgaaga tcatcctttt taggaaattg aagcttttta tgttgcacaa tgcatttata
     3061 atactacaat gatgttttca ccagatattc ttctagggtg cggtgtaatg aaacagatac
     3121 acttgcaaaa gaaaattcac cgcgaattta ctaaaattat gaataattat gttaatcttc
     3181 cgaaattttt ttccaaaatt aagggatcaa gctggtgtta tagattgttt aaggttggca
     3241 aaaaaataac ttctttttaa acaattatag aaaaccgata ctctgttaag ttggttgaca
     3301 cggtgtcgat ttttatgact ttcttattct gaaacttcac taacggacag tacctaaaaa
     3361 gtctatagta tggacgttta cgagtactat tttatcaaaa atgctgctca tttcaactta
     3421 tatgttttag tgaactaccc ccacttag
//
LOCUS       DAZKCY010000184         3378 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000184 DAZKCY010000000
VERSION     DAZKCY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.189"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..660
                     /locus_tag="ACG8XC_002685"
     CDS             196..660
                     /locus_tag="ACG8XC_002685"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9192334.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            674..1423
                     /locus_tag="ACG8XC_002686"
     CDS             674..1423
                     /locus_tag="ACG8XC_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9192335.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            1423..2253
                     /locus_tag="ACG8XC_002687"
     CDS             1423..2253
                     /locus_tag="ACG8XC_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9192336.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            2253..2900
                     /locus_tag="ACG8XC_002688"
     CDS             2253..2900
                     /locus_tag="ACG8XC_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9192337.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(2998..>3378)
                     /locus_tag="ACG8XC_002689"
     CDS             complement(2998..>3378)
                     /locus_tag="ACG8XC_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HFP9192338.1"
                     /translation="MWEDGAPMGIDNFFASEEKFPTLFEKGSQYTSLYKLLEDDYDVK
                     ATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQPFHYSQSTVRCDRISY
                     VIEVTENITLDTVHKNDSDPKVID"
BASE COUNT         1130 a          451 c          632 g         1165 t
ORIGIN      
        1 ctgggattaa tttacctctt ttactggagt tattaatggc ccagtcagct ggtaaatcta
       61 tagaagaaat agattttaag gaattactga ataactctca aaaaagtatg gttcatatta
      121 attcgctttt aaaagatgtc agttaaggtg gcaatttcta ttctataaat tttaaaaatt
      181 gaaggaggaa atagaatgat taaattagtg cgtgtagacc acagattact tcatggacaa
      241 gtaatatttt cttggactaa acagtatgat attaaccata ttattgtggc tgataacaaa
      301 gttccaggag atccaatgtc tgtgatggct ttgacaattg ctaaacctgc tgattgcaaa
      361 ttagacattg ttaaaattag tgatgttaaa gggattatgg aaacatcccc aaatgataat
      421 tacatgattt taattaaagg acctaaggaa gcttttgaat tagttcaaaa tttaccagaa
      481 attaaagaaa ttaattttgg cggtgtgtct aagaaaagcg agtccaaaca atatggaaag
      541 gcagtctttt taactcctga agaattagaa gccacaaaaa aaattcttga tcaagaagtg
      601 aaaatatttg tgcaacaagt cccatcttca agtattgaaa atgtagattt tagtaagtaa
      661 ggggggaaaa taaatgttat taaaagccat tttattaggt ttagttggtg tggtagctgt
      721 tattgactcc aggttaattg gtcgacaaaa tattggtaga ccactaattt tgagtacact
      781 tgccggactt gtgctaggtg acttgcatac agggattgtt cttggggcat ctttagaatt
      841 aatttctatg ggattcgtct caattggggc ggctggacct cctaatatgc aacttggtag
      901 tattattgcc actgcttttg caattttaac taatagtagt acagaaacag ccttaacaat
      961 agctatccca gtggctgtag ctggtgaatt tatgagtatt atcatgcgca tgataattgc
     1021 tcaatttgga catgctgcag ataaagccat tgaaaataat aaatttagaa aagctcaaat
     1081 tattcatatt tattggtcat ttatttttaa tgcttttgtt tactttattc caatcttttt
     1141 aacagtatat tttggtgctg acgttgtggc agatttagta gataaaattc cagaaatgat
     1201 tacagacgcc ttgactgtct ctggaaatat gctttccgca ttagggtttg caatgctatt
     1261 aagcacaatg ttgagtaaaa aattatatcc ttactttatt tttggattct ttattgttgc
     1321 ctattcaggt ctttcactaa ttggcgtgac aatttttgca gctctgattg cctttgtaat
     1381 ggatcaagtt aaatatggaa aaagagaaga ggcgcatggt taatggaaaa tagtacagaa
     1441 aaaaaacgtt taatcacaaa aaaagatctt aataagatgt gtcttcgttc atggacaatc
     1501 atgtgttcgt ggaattatga aagacaaatg catatgggat ttatgtatgg gatggcgcca
     1561 attttaaata aagtttataa aaatgatgaa gagaaaaaaa gagaagctta tactcgtcat
     1621 atggcatttt ttaactgtac gcctcaaatg acttcattta ttatgggact ttgtgcttct
     1681 atggaagaac aaaatgctca agcagaagaa ggaaacttta atactgaatc tatctccatg
     1741 attaaaacca gtttaatggg tccttttgct ggaattggtg attcattttt ccaaggtact
     1801 ttaagaatta ttacatttgg gattggattg tcttttgcta agcaaggtag tattttagga
     1861 ccgattttag ctgttggcct ttttgctata ccttctattt tagctgtttt ttacggcggt
     1921 aaattaggat atgagtctgg caataaatac cttactaaat tgtatcaaga aggtcttatg
     1981 gatcgagtaa tgcaatttgc ttctatcatt ggtttggctg tagtaggtgg aatggtggct
     2041 agcatggtgc caattgtcac ccctttaaaa ttcactacag ggggaacaga acttgtttta
     2101 caagacttac ttgattccat tattcctgga ttattaccac ttgtatgtac tttgggaata
     2161 tataaattga ttaagaaaaa tgttagtaca aatatgctct tattaggttg tattttggca
     2221 gggtttgttt taagtataat aggtatctta taatgattaa ggctctaatt tttgatatgg
     2281 acggagtttt aattgatagc gagattatgt atcaaaagtg ggtatttaat tttttaaaac
     2341 aagaaaattt aagctatcct ttgtattctt atcaaaaaaa gattggaact acagcaagta
     2401 tttttgatga tatagaaaag tacaatgagg gatgtaacag tttagaattg aagggaaaat
     2461 ttactaaata ttggaaatcg caaaagattg attacttgga tatttttcct aatatactaa
     2521 ttgaacagct tgaatggtta aaaaagaaaa aaataaaaat ggcaattgct tcttccagtc
     2581 caatggatac tataaatgaa gttgttacca cttgtaaaat agagtcatat tttgattgtt
     2641 taattagcgg aagagatttg ccagaaagta aacctaatcc tactatattc cttaaagctt
     2701 cagaacagct cagaattcca gttgaagaat gtctggtgat agaagattct tataatggta
     2761 taaaagctgg taaaagagca aatatgaagg ttcttgcaat taaagataaa cgtttttctc
     2821 aagatgtaag tctagcagat gatgttattt acgatattaa atatctgcga caagaagtac
     2881 aggtgaaatt tggaatataa ataagttttt tgatgtattt agagaatatt agtaaaaata
     2941 aaaatcatgc ccaaatctaa tcaaaattga ttgctttggg tatgatttct tttaatatca
     3001 atctattact ttaggatctg aatcattttt atgcaccgta tctaaagtga tattttctgt
     3061 gacttcaata acataactaa ttctatcaca acgaactgtg gattgagaat aatgaaatgg
     3121 ttgattattt ttatctttgc caaccttttc taaaataaat agcggatctc ctactataca
     3181 ttgcaataag tcggaaagct caggtgttgc agataaaccg tttatttcca taatagagct
     3241 cgtagctttg acatcatagt catcttctag aagtttatat aatgaagtat attggctacc
     3301 tttttcaaat aaagttggaa atttttcttc tgatgcgaaa aaattatcaa ttcccatcgg
     3361 ggcaccgtct tcccacat
//
LOCUS       DAZKCY010000185         3359 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000185 DAZKCY010000000
VERSION     DAZKCY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.190"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..1175
                     /locus_tag="ACG8XC_002690"
     CDS             96..1175
                     /locus_tag="ACG8XC_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9192339.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(1476..1718)
                     /locus_tag="ACG8XC_002691"
     CDS             complement(1476..1718)
                     /locus_tag="ACG8XC_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9192340.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(1750..2307)
                     /locus_tag="ACG8XC_002692"
     CDS             complement(1750..2307)
                     /locus_tag="ACG8XC_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9192341.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(2320..2508)
                     /locus_tag="ACG8XC_002693"
     CDS             complement(2320..2508)
                     /locus_tag="ACG8XC_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP9192342.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(2521..3087)
                     /gene="amaP"
                     /locus_tag="ACG8XC_002694"
     CDS             complement(2521..3087)
                     /gene="amaP"
                     /locus_tag="ACG8XC_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP9192343.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT          927 a          767 c          679 g          986 t
ORIGIN      
        1 taattaactt agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag
       61 ttaccacaac tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag
      121 tttcacgcaa gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa
      181 aggaaaacta ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa
      241 acaacgaaat caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg
      301 tctacgacta ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga
      361 accgcttgaa ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg
      421 cggacgacat gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa
      481 aacacgaatt gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg
      541 accgtggcat catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga
      601 aatcgtcccg tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc
      661 gcccggaggc ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag
      721 gaaacaagac caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg
      781 aggtcattct gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc
      841 tgagagaacg ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg
      901 gctcggagtt cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc
      961 acccctatgc ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt
     1021 tcatccccaa gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc
     1081 gttggatgaa cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg
     1141 tccaggcatt taggcaggaa cggttgacgg cttgacacgt gggcccttga caacgagcct
     1201 ccctcggcat ggcttgtgtc tggaaagctg gccggggcca gccagtcggg agactaccat
     1261 gcctcagctc attgtcaagg gcccactcga ttagaaaaat tagacggttg gtggtaacga
     1321 gcaaccgggt caccatgggt ggctaactta aacttgaaat ttacgtaagg aataaagaat
     1381 acttcatata aaatatataa aaaagaaccg cagcgagtgt cgctacggtt ctcaaatttg
     1441 ttcttcattt caacataagt ttttcttcaa tttgcttata atttacgtgc aaagaatgaa
     1501 acgactaata ctaaaatgat tgcaccgatc aatgatggaa tgatagccat tcctgcaatt
     1561 gttggtcccc aagttcctag taaagcttgt ccaattgctg aacctactaa accagcaatg
     1621 atgtttgcga tgatccccat tgatgatcct ttattcgtga ttgctcctgc aattgctcca
     1681 atgataccgc cgacgattaa tgcccaaata aatcccattt taaattcctc ctaactgact
     1741 tcttacttct tattgtacac gtggttcgtt attttctttg actttttgtg cacctttgcc
     1801 tacagcagat tttgctttat ctgtttggcg tgaagcaaat tgtcctgtgg attttgctgc
     1861 ttctgttact ttgtcttgta ctgtttcttg atctttttgg tattcttctt gtgttttgat
     1921 gtctgcaaca ttcacattca cttcgataac atctaaatgt gtcatattgt ttacttcagt
     1981 actgatcaat tctttgattt ggttgtaaat atcttcgatg tctttaccgt attctacgat
     2041 cacatctaga tccacagcta cttgtttctt acctacttct gtattgatac ctgctgttac
     2101 gttatctgtg ttgactaatt tttcagccat atttgagaaa aatccgccat ctaatgtcaa
     2161 tagaccatct acatctttca atgcataacc gatgattttt tggatgactt tgtcttcata
     2221 agtcaattca ccattcatca tgcggttttc tcctttttca tttgccaaaa ctgatccatt
     2281 tgttgtattc atgtttttgt tttccatctt tgtttcctcc tatgatttaa aaaattgatc
     2341 aactattcct gactgtttca aataaaaacc gattcctgcg ccaattgctg tcatgattat
     2401 gatcaacaat gtcttgaaaa atccaatcgt taaccataaa acagctagca gtagtccaag
     2461 acccccaaag ataataggga gcttgtatgc tgaaaatagt tctttcatta tttggccctc
     2521 ctattccact cgagagtgct tgtagtctct tgtatcttct tgttcttcat aaccgctgta
     2581 tgtgactgcc actttgaccg tttcttttgt tccgagtact ttagttactt cttcttcgat
     2641 atcttgcatc aatacacctg ttttccctac taatgaagaa gttcgtttca agtctccgtt
     2701 gacatggata tggattcgat ttttcgttga acgtatccgt actttaggtg aatgaatgaa
     2761 ctcttcttcg tgtaaatggg aacgtaccaa cccttcgatc gcttttttgt cgattgttag
     2821 tttgccatct tcccgtttca taacgaaagt tcctcttgac tttggataga aaagaacgac
     2881 tagcaagatg ataatagcaa gaacggcaaa tactacagct gcccagaaaa tatattgttg
     2941 catgtagtaa ttcgttaaag taaagaagcg aaatcgttcc aattgttcag gcataatata
     3001 cggcgcattg atcaacgcaa tcgctagaaa tactgaaagt agcagtaaac tcatgataac
     3061 accgattatt tttaacccct tattcatttt ccacctccta taaacttatt tttaccctgt
     3121 aaataactct accgacaatc acagtataga tagcgaacag ttagcatagc atcagtaaat
     3181 aaagcgctac caaacattgc tggatcataa cggcgtttta ttgccagtct gatactgaaa
     3241 taacaattgc ttacttattc tctatttact tttgattgct ggctatactt aaaggatatt
     3301 attcaagatt aaaaattgca aagattatgc tcagttatta ttacaagatt ctatttatt
//
LOCUS       DAZKCY010000186         3308 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000186 DAZKCY010000000
VERSION     DAZKCY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.191"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..85)
                     /locus_tag="ACG8XC_002695"
     CDS             complement(<1..85)
                     /locus_tag="ACG8XC_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9192344.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNS"
     gene            246..935
                     /locus_tag="ACG8XC_002696"
     CDS             246..935
                     /locus_tag="ACG8XC_002696"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9192345.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            949..2451
                     /locus_tag="ACG8XC_002697"
     CDS             949..2451
                     /locus_tag="ACG8XC_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9192346.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2464..2940
                     /locus_tag="ACG8XC_002698"
     CDS             2464..2940
                     /locus_tag="ACG8XC_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9192347.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(3037..3147)
                     /locus_tag="ACG8XC_002699"
                     /pseudo
     CDS             complement(3037..3147)
                     /locus_tag="ACG8XC_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1099 a          449 c          626 g         1134 t
ORIGIN      
        1 cagaatttaa aataaaatca gcgacttttt tttcagactt cgttaaaaat ggataatttt
       61 ctttaattaa atagatataa ctcatacaaa ctcctctaca atttcaactt ttctccatta
      121 tactccaaaa taataaaaaa aactcctaaa tactccattt ttatctttac ttttatgaaa
      181 gcggatgcta tattgttttt ggagttgtta tccaaaaata ataaaaaatt ggagtgttta
      241 ggataatgga taaaaagagg tttttagatc ttatttcaaa aggtttgatt gtttcctgtc
      301 aagcattacc tggtgaacca ttttacaaag aggaagaaag tttaatgcct tattttgctt
      361 tggctgctca aagggctgga gcagttggta tacgtgcaaa ttctgttcga gatattattg
      421 aaattagaaa gtgtgtatca ttaccaatga ttggaattat taaaagagat tatccaccag
      481 aatctccatt cattactgcc actatgagag aagtagatga attagtagaa accggtgttg
      541 aggtaatcgc tcttgattgc actcttagaa atcgacatga tggactatct ataaattctt
      601 ttattactaa aataaaagaa aaatatcctc atcagttatt tatggcagat atttctacat
      661 ttgaagaagg caaaaatgca tataaagcag gaatagattt tgtaggaact actttaagtg
      721 gatatactga ggaaagtaaa aaacaagatg ggccagatat agatttgatt aaaaaattgg
      781 tagaagaagg gatttgtgta attgctgagg gaaggattca tactccagaa caagcaaaag
      841 agataaaaaa aataggagtt gcaggtattg ttgtgggtgg tgctattact cgaccacagg
      901 aaattgcatc aagatttgtt aaagcattaa gttaggggga gaaataaaat gtttaaaaaa
      961 ttttcacaat taggacgcgc tttcatgtta ccaattgcta tattaccagt agccggatta
     1021 ctattgggac tgggaggagc gttaacaaat gagagtgcta taaatgctta tccattttta
     1081 gatcaaccgt ggttgcatac aattttaagt attatgagtt atgctggaaa tgcagttttt
     1141 gcaaacctag cactaatttt tgcaattggt attgctgtag gacttgcaaa tggagataaa
     1201 ggaacagctg gtttagctgg tggagtagct tatctagtat atactgcaac tattagtggg
     1261 tttcttgaac ttttttcagc aaaagatgca acactagata caggtgtagt gggatcaatt
     1321 gttgttggtg gtactgttgc atttttacac aatcgttata gaaaaattga attaccacaa
     1381 ttcttaggtt tttttggagg atcaagattt attccgattg tttctagttt agctgcaatc
     1441 attattggtt ctttctttta tttaatttgg ccaccaattc aaaatggttt agtagttgct
     1501 ggtgaacaaa ttgcccaaat gggtagttta ggaacttttt tatatggttt tttattgcgt
     1561 ttaacaggtg ctgttggact acaccacacc atttaccctt tgttctggta tacatcttta
     1621 ggtggtaccg aaactgtagc tggatcaaca attgctggag cccaaaatat tttctttgcc
     1681 caactagctg atccaaacca cactggtctg tttacttatg gtactagatt ttttgcagga
     1741 agatttgcaa caatgatgtt tggattacca gcagcatgtt atgcaatgta tcgtgcaatt
     1801 cctaagaaaa atcgtaaaaa gaatggtggg ctttatttta gcggggccct tacatcattt
     1861 ttaactggaa ttactgaacc agttgaatat atgtttttat ttgttgctcc ttggctttat
     1921 gtgattcatg cattcttaga tggattatct ttttattttg ctgatatctt aaatattcgt
     1981 gtagggaatt ccttttcagg tggtttgatc gactttttat tattcggtgt acttcaaggg
     2041 aatgacaaaa ctaattggtt aaaattaatt ccttttggta ttgcatgggc atttatatat
     2101 ttctttgttt ttacattctt tatcaaaaaa tttaaggtag ctattcctgg aatggaagaa
     2161 gaatcagaag ttcattctga aaaagtaata aacaaaaata catcattaca tgaagagtct
     2221 cttatcatta ttgaagctct tggtggaaaa gaaaatattg agtctgttga agcttgtgca
     2281 actcgtttgc gtgttgctgt tacgaatggg gagattattc aaaaacaaac aataaaagac
     2341 actggtgcaa ctgctgtctt cgaagtgaag ggtggcatcc aagctgtttt tggagggaaa
     2401 gctgacttat taagtcaaga aatcaatcaa atattaggaa aagataatta ggaggttttt
     2461 aatatgtttg aaattttcaa aaagaaaaaa caagaaactt tttatgcacc ttgttccgga
     2521 aaaatcattt ccatcacaga agtaaatgat cctgtctttt cacaaaaatt actaggtgat
     2581 ggttttgccg tcatacctga tggttcaaaa atttatgctc cattggctgg tcgagtaaca
     2641 aatatatttc ctactaaaca tgctattaat ataaaaagca actcaggcat tgattatttg
     2701 attcatattg gaattgatac agttgaatta caaggagagc ctttttcgat ttgtgttcaa
     2761 gaaaatgatg aggttactcc tgaaacctcg ttggttgatg tggattttga caaaataata
     2821 aaatctggta aagatacttc tgttatagtt gtttttccgg agaaaaatca gttcaatgat
     2881 ttaataatag ataatggtcg aattgctcat gggaattcat gtggaaaaat cactttttaa
     2941 aaaagtgtat ataaaaagct ataaaataag ctaaatagga tatttgtcaa atgaagttgg
     3001 tgaacagaaa gaactgtttt ctttctgttc atttttttat tttattagta atgattcccc
     3061 atgccaaatc atgatccgta atttaaagtt ataaaagctt cgaaatccat tggcatttcg
     3121 ttggaatacc ttgatatgat tattcaaata taaaatatag agagaagaga gagagggaag
     3181 atatttataa agggcaaagt tatgtttact caacttagga aatagaaata gatcaattta
     3241 tattctcttg tcaatcgaat atgaacctta gcactatata ccaaatatag taaaatgctg
     3301 aaagatgt
//
LOCUS       DAZKCY010000187         3292 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000187 DAZKCY010000000
VERSION     DAZKCY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.192"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..690
                     /locus_tag="ACG8XC_002700"
     CDS             <1..690
                     /locus_tag="ACG8XC_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9192348.1"
                     /translation="TKVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKY
                     YDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLR
                     VGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITN
                     YIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREK
                     VLRSIIDKSIE"
     gene            920..1150
                     /locus_tag="ACG8XC_002701"
     CDS             920..1150
                     /locus_tag="ACG8XC_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFP9192349.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1373..1471)
                     /locus_tag="ACG8XC_002702"
                     /pseudo
     CDS             complement(1373..1471)
                     /locus_tag="ACG8XC_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1736..2191)
                     /gene="tnpA"
                     /locus_tag="ACG8XC_002703"
     CDS             complement(1736..2191)
                     /gene="tnpA"
                     /locus_tag="ACG8XC_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFP9192350.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2434..3292
                     /locus_tag="ACG8XC_002704"
                     /pseudo
     CDS             2434..3292
                     /locus_tag="ACG8XC_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT         1082 a          575 c          575 g         1060 t
ORIGIN      
        1 acaaaagttg gcgatgctac tattattcgc ttttgtcaaa aaattggttt ttctggtttt
       61 tctgatttaa aaattgaaat tgcaaaggaa gactttagtc aaaaaaaaga acatccgact
      121 agtgtaaagt actatgatga aattgctaat agtttaacag aagctctcta ttccactatt
      181 aggcttttag acgaagaaaa attgaatgaa gccattaaaa tgatcactca atcaaaaaac
      241 atatatatat ttggagtggg atctagtgga aatactagtc ttgatttaga aaatatgttt
      301 cttcgcgtag gagttcaagc aaaagctgta ctagaccctc attttcaagc ccaagtcgct
      361 tctttattaa cagttaatga tttagtaatc attttttctt tatctgggaa aacgaaagat
      421 acttatgatt cattgaaaat agctaaaaaa aatggcgcaa aaattattgc tataactaac
      481 tatattcatt cacctatagg aaagtctgca gacttagttt tacaaacagc cattgaagaa
      541 tttcttaatg gtggatcttt agctggaaaa atatcacaac tttatatttg tgatctatta
      601 gttcatggtt atgaacaaaa caataatatt gattcagttg aattaagaga aaaagttttg
      661 cgctctatta ttgataaaag tattgagtga actaccccca cttagctaaa cctaacggtt
      721 tttaacgatt gaagtaggca attcttggga tgagagcata cttgatagct tattttttac
      781 taatagcggt ggctcttatt gaccaaatat ccctgtagtt cctacggttc ttgattttat
      841 ctaaagatat ttttatcgtg gaagctattg atcaacttga aagaaaaacg tgtagctcat
      901 tatcactagt ttatcgagta tggatgaagc aatggatcat cctctcttaa ataaatttat
      961 taaggatctg atccttcaaa ttttttccat gatcgatgag caggaaccta ctgaatctaa
     1021 acgacatcgg gcacaaggaa ttattaagat tgctaagcga atgttttcta taaagggtgt
     1081 tctaagctat acagtgttaa ttcaaaagat tcccaatcat cttgtttata agaacaacgt
     1141 tgaagattaa aaaagtgttt caatgctaaa attgcaaaat acttcaatgt tatgagacaa
     1201 actgtctata agaatcaatt tccacccatt tcatcacttg ttaaaagttt atgatccttt
     1261 tgatttttat attaatttat ctgtatttaa tacagatttt atttattagt tcatctgcct
     1321 attgtatttt attttgttgc cttaccatct gaagtaaata tactcttaat tgtcaaatta
     1381 agttagacag atcatcaata cttacgtaac tcaaaaatac ttccaaaggt gttcgataat
     1441 ccagtgattt tctaggaaga ttatttctgc gccagcattt tctaatgttt gaatttgtgt
     1501 tttgtaatct tggttgcttg tgctaattct tgaatagccg taaattgcca aaatttcatc
     1561 accctttctg gacataagtt tctgacacca gttaaaatca agtctatcag taacccatac
     1621 acccgtgagg acgggtgtat ttttaatccc cctagaaggg gtcgacactc gctgcccctt
     1681 aaaaggggat ttttaaaaga ccgccaactg ctcatcttca ctcacttact tctctttatt
     1741 ttttaaatgg ttctacgtac tctcttttac ttataatata tcgaatgcga tcatcagctt
     1801 cttgttcacg gatgtatttt tcatttgtct tttgattcaa tccgactgta ctcacatagt
     1861 atctcttcga ccaaaaggat ttgtttccgt atttatattt gagatttgcg tgctgctcgt
     1921 ggattagtac cgcactacga cctttcagat accccataaa actggatata catatttttg
     1981 gtggtactcg aacaagcatg tgaatatgat ctagcattgc atgtgcttct atgatttcca
     2041 catctttcat ttcgcacaac ttcctaagta tttttccaag atcttttcgt aattgcccat
     2101 agattacttt tcgttgatat tttggtgtaa aaataatatg atacttacaa ttccaacgag
     2161 tatatgctaa acttttatcg tcatttgaca tgaaaaaact cctttcgatc tgatgagcgg
     2221 ttggtagcca ctcactagta tatcgaaagg agtttttatc gctaaagata cttcttccac
     2281 gcgtagaaac gcgcggtttt cttgtttcgc acgctttacg cacaaaactg actgaagtca
     2341 ttaagaaaat aaaagggtct aaaactttta agttttgtac cgacccccaa aagttagacc
     2401 aaaaaatcta acttttggag gtcggtattt tttatggcta agtatacttt tgaacttaaa
     2461 ttaaagatcg ttcatgatta cttggatggt aaaggcggaa gtgattatct ggcaaagaaa
     2521 tattccatta aggctccatc acaagtgaag cgctggatca atgcctatca agaatttggt
     2581 gaagagggtc tagtaagaaa gcgacaaaag aaaaaatatt ctgttcaatt taagctggat
     2641 gcgatagagt tatatctaac aagtgagctg tcctatcgtg aagttgccaa cacacttaac
     2701 atgaacaacc ctaatttgat cgctagttgg atgcgtcaat tccgtgaggg tgggatcgat
     2761 ggactctcaa aaacgaaagg atgtccacct atcttgtcta agaaaaatga acccaaaaat
     2821 aagaaaaaat ctacaactaa agcaacgtct aaagaacgtg aacgcatcga ggagttggag
     2881 aaacgagtcc gttcccttca aattgaaaat gcctttttaa aagaattgag gaagctgcga
     2941 aaacaggaag cccaacaacg acgaatgaag caatcgcacg aatcatcgca agcctccgag
     3001 gaccgtttaa attagttgag cttctagaaa ctttaaagtt ccctaaggca atctttatgt
     3061 actggcaaaa acgattggac cgtaaaaatc cagatcaaga gattgaagcg gaaatgctta
     3121 aagttcggga gaagcacaag gattatggct gtttacggat gaccaatgaa ctaagaaacc
     3181 gcggtttctc tatcaataaa aagaaagttc agcgcctgat caagaagcta ggaataaaag
     3241 taacgacata cacacgtaaa tcacggcgct acaattctta tcgaggacaa gt
//
LOCUS       DAZKCY010000188         3226 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000188 DAZKCY010000000
VERSION     DAZKCY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.193"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1338)
                     /locus_tag="ACG8XC_002705"
     CDS             complement(139..1338)
                     /locus_tag="ACG8XC_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9192351.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="ACG8XC_002706"
     CDS             complement(1468..2844)
                     /locus_tag="ACG8XC_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9192352.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..>3226)
                     /locus_tag="ACG8XC_002707"
     CDS             complement(2844..>3226)
                     /locus_tag="ACG8XC_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9192353.1"
                     /translation="DYLSPIENIRLVNKSADESILFELGLDKKQIKRNVMKLSGGQQQ
                     RVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVAD
                     SADIILELSGKKLKKVNKMNLEVE"
BASE COUNT          989 a          614 c          457 g         1166 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacgtacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaat attttcaatt ggtgataaat aatcta
//
LOCUS       DAZKCY010000189         3205 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000189 DAZKCY010000000
VERSION     DAZKCY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.194"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2113)
                     /locus_tag="ACG8XC_002708"
     CDS             complement(<1..2113)
                     /locus_tag="ACG8XC_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFP9192354.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTSNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATPIEFEIERSQTAVVELTK"
     gene            complement(2785..3053)
                     /locus_tag="ACG8XC_002709"
                     /pseudo
     CDS             complement(2785..3053)
                     /locus_tag="ACG8XC_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          876 a          717 c          518 g         1094 t
ORIGIN      
        1 ctttcgtcaa ttctaccacc gctgtctgac ttctttcgat ctcaaactcg attggtgtcg
       61 catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta cctacaggaa
      121 gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc acttgtgaat
      181 cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca tcagttttaa
      241 ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc aatcgattgt
      301 cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac gcctctacat
      361 tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc gcactgaaac
      421 gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat agaaatgaca
      481 caaattccat gccttcttcc aaaacatctt caataacaag cgtattgctt gttccagaaa
      541 acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata acatttttgt
      601 ccacactttt tcttaaagag attgccggaa gattactttc agggattctc aagcggtagt
      661 ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa aacccatcct
      721 cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat tcaataaaat
      781 acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt tccttattaa
      841 aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt gcttcacctg
      901 gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt ccagtggaac
      961 tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg gtaaccacta
     1021 caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta ctgttggtca
     1081 cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt tctctagagc
     1141 catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt gaaaattccg
     1201 tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt ggctcagtac
     1261 cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac tgagcggctt
     1321 catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct tcttggtaaa
     1381 atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac tcaattaagc
     1441 caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga atatcgccag
     1501 cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa gtggggcgct
     1561 cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc tgagattcct
     1621 gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag ctttcttctg
     1681 gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca cttgtgcttt
     1741 cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg cttcttgcgg
     1801 tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg taatcattga
     1861 gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat gcatttagca
     1921 ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc tcctcttgat
     1981 caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct agaaccgtta
     2041 tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac agtttatgaa
     2101 atttgttcct cactattact cccccttata attttgacta aacaacaagg tacccgataa
     2161 ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta ttttcggttc
     2221 aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt gtcttctgtt
     2281 gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc ataattatga
     2341 tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta atagttattt
     2401 tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta aatcgttgat
     2461 accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac cttcatcaat
     2521 cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga atacttggga
     2581 ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc aactaaacgc
     2641 atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta ctaataaact
     2701 gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc tttttgctct
     2761 ttatttgggc gtagttttac tttcttaact tgtatcgtct tccatcaact ccttaatcat
     2821 cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag tgacaatctg
     2881 tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta ccatatattg
     2941 caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta tagaataact
     3001 accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc ttttgttgta
     3061 gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg tattgctatt
     3121 tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca ttgattcccc
     3181 tctaaaatta ctatccataa aaaat
//
LOCUS       DAZKCY010000190         3125 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000190 DAZKCY010000000
VERSION     DAZKCY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.195"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1040
                     /locus_tag="ACG8XC_002710"
     CDS             72..1040
                     /locus_tag="ACG8XC_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9192355.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            1050..1538
                     /locus_tag="ACG8XC_002711"
     CDS             1050..1538
                     /locus_tag="ACG8XC_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9192356.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            1834..2115
                     /locus_tag="ACG8XC_002712"
     CDS             1834..2115
                     /locus_tag="ACG8XC_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192357.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            2117..2533
                     /locus_tag="ACG8XC_002713"
     CDS             2117..2533
                     /locus_tag="ACG8XC_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192358.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         1249 a          540 c          477 g          859 t
ORIGIN      
        1 attagggggt tgacccccat tagaaagctc tttataattt tgttaagtag ttaatcttga
       61 aggaggattt tatggcaaat gaaattgtga aataccacca tgaattaaat acgattcctt
      121 tacgcaaatt tacgtcagta gaaatgaatt tattcttctc tataatatct agaatgcgtg
      181 atgtaggcga taaaaaagta caatttacat tcgaacaatt aaaagattta agcaattata
      241 aagcaactgc aaatgttcga ttcatagatg atttagaaac aacgtatgac aaattaatgg
      301 atttacgttt tggtagaaga agtgctgacg gtttgcaacg tgaacgattt gttttattta
      361 atcaatttaa aatcgatgga aaagctgata ttccttttgc tgaaatacaa gtccatgaga
      421 aagccttacc cttacttaat aatcttgaag aatgggttcg atattctctc caacaattca
      481 atgaattaga aagcagttat tccaaaacca tgtttagatt attaaaacaa tacagaacca
      541 aaggaattgc caatttttca aaagaagact ttcatgaatt acttgatatc cctaaaagtt
      601 atagagaaag tgatataaat aaaaaaatta taaagcctat tcgccaagaa ctaaccgcaa
      661 tcttcaaagg tttgacaatc caaaagagat acggaaaagg acggggaaag cctgtcattg
      721 gctatcaatt tacatttaaa cctgagataa aaaaatcaga cgactttaac aaacatgctc
      781 caaccaaaga agaacaaaat aaagctttgg aactagctaa tcaacaggtt caatctgaaa
      841 aagaacctac ttcattacaa gaaaaaatca aagaacaaaa acgaaaatta gaacttttac
      901 aacagttagc agaattagaa agaaaacaaa ataccccaag taagaaaatt aacaaacaaa
      961 tagcagataa caattccgat attaccccag aatttaaaga aaacttacta gataatctcc
     1021 aaaacttatt taaagaataa ggggttttta tgacagataa aactatcaaa gaattagcag
     1081 atattctagg tgtttcaaaa gataaagtga agtatcaact gaggaaatta cctagtaatt
     1141 ttacctacaa aaaaggaaat attacctacc tgaaaaaaga agcagtaaca acaattacaa
     1201 aggttttatc aggtaatgaa tcgggtaact tacctagtga attacccgat tcattaccta
     1261 ccgattacct actcaaggag attgaaacta agaataatca tattgaagaa aaagataggc
     1321 aaattgaaaa acttcaaata gctttagaaa cgcagcaaaa attactcgac cagcaacaac
     1381 aactcaccct acaagccaat caacaaatca aaaaacttca cgaacaactc cagctaacct
     1441 atgcagaaga atcttcagaa aaagaacccc tttctgttac tgaagaaact caaaaaggac
     1501 aaaacaagga gaaaaaatgg tggcaattct ggcaataacc caacaatttt tattcataaa
     1561 aattgttggg ttactttctt tttttgcttt tacaagcata gcgacatgat ctcacgtcgc
     1621 tattttttaa agagcataag gaaacgcggt atcactttct tacgaaaggt gctaggtagg
     1681 ctgtgcctac cacgtttcta agaaaaaaat ttgctatcac tttttcgtca ttgtggatag
     1741 cttatgtata gtgtttcggc aaaaaacagc tatacaaata ctatatttgt atgatataca
     1801 taaattatac acaaatgcga aaggaattct tctatggaca ttttagttcg gaatattgat
     1861 ccaaaagtta tttcagaaat agatagacgt tgccaagaac tcacaacaaa aactggcaaa
     1921 aaatggagcc gtaatgcgta cctaaaagtt ctaatcgaaa acgattttga ccaggcactt
     1981 aggcattaca aaaaagatca attcgattta ttactcgaaa aatttatcga tatacaagca
     2041 aaaaatactg aaacattgaa cgactatgtt catacaaaca accaactcat tgaaacactt
     2101 atcggaggaa tataacatga gaaaaacttc tgttcaaaaa cacgttaccc tttatcccag
     2161 tcacattgac gttttagatt ccatcatcga aaaaaatcca gatatcaata actactcgga
     2221 agccattcgt tttctatgta tgagttatga acctaatact aatacgacaa ctaccagcaa
     2281 ccaagcaaaa ctaaatgcga tgggaaaaga aatttctatt atgtccgaaa tcattgcaga
     2341 atttgctgat ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtata
     2401 tcaagaagtg aaagaaaaaa ttgaaaatca actcaatccc tcccgacgaa atcaacccaa
     2461 aaatcgtcaa aaagaaacca atcaaacatt cacttctaga aattttgacc caacaaactt
     2521 tctaaaaaga tgaaaaagaa accgaaaatt ttttctattt ctcagtttct tttttagggg
     2581 gcagtcttcc agcaagattt gcaagttacc ggtaacttgc acaaaaaaag aaaaatccgc
     2641 ttgtgcgaat ccttcttttt gcattttggg gcgctgcccc aatctccaaa tatcggttat
     2701 ttaatagcgt ggtaactcaa aaaaatagaa cgtactctga aataaaaaaa gactgctcta
     2761 acgagcagcc aataaaaaat aatgagcgtt tattcttgaa aaagcagcgc gcttatgaag
     2821 cgctgccaaa aatttaccat attttatgaa aaataacaag tttctaaaaa ggaatcggtt
     2881 aagattcaca aagaatcgta aacagacaat agcggaaaat caagcttccg aacgtacctt
     2941 ttagctagac caatgaaaaa tcacaaagtg agcgtctcta tccattggta gaggggctac
     3001 ttggcatagt gtacattatg tctactagct cgataaataa cgattatgtt cccaaaacaa
     3061 tattttatga actataacaa aaatttgtta tcgcatattg tatattttaa ttataggtgt
     3121 atatc
//
LOCUS       DAZKCY010000191         3103 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000191 DAZKCY010000000
VERSION     DAZKCY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.196"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG8XC_002714"
     CDS             316..570
                     /locus_tag="ACG8XC_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9192359.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG8XC_002715"
     CDS             571..888
                     /locus_tag="ACG8XC_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9192360.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACG8XC_002716"
     CDS             1447..2382
                     /locus_tag="ACG8XC_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192361.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
     gene            2476..3103
                     /locus_tag="ACG8XC_002717"
                     /pseudo
     CDS             2476..3103
                     /locus_tag="ACG8XC_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
BASE COUNT         1120 a          464 c          570 g          949 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagagaaaaa
     2401 tgtctaaaca tgttggtaaa tcaacaaaaa aacgcatata gtagtctcaa aactcccaag
     2461 aaatgaggac tacatatgcg ttcatcaaaa tatactgaac attatacaga tacttttata
     2521 acattcaagg aaattatgag aactgtttct aacatatacc acaattgtgt accagataag
     2581 attaaaaacc gaagaaatac tgatcaactg aagtaacgcg atacagtgat tattgcttgt
     2641 gttatatggg gcataattaa tggctatact agccaaagag ccacgtatag agcggtttgt
     2701 tccgtcttat ttcctaatgg tgactttcct agtaggagtc gattcactcg cttaagttca
     2761 aacttagctt acactatcaa gattattcga tactttttca tcaagaagct caccaaaggt
     2821 gaactagtcg gaattataga tagttttcct agtccattat gtaaaccagt tagaaataga
     2881 caagcaaaac tattgaatca aatagccaaa gttggctata actcaacaaa aaaatcttat
     2941 ttttatggtc ttaaaattca tatgatcgtt actaagactg gctttccgat tacttactca
     3001 atcacgaatc caggtgtcca tgacgtgaaa gtgttagaga ctttatcgga agaagcgaat
     3061 cttcccaata tcctagggga caaaggatat attagccata aaa
//
LOCUS       DAZKCY010000192         3102 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000192 DAZKCY010000000
VERSION     DAZKCY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.197"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..759
                     /gene="fabI"
                     /locus_tag="ACG8XC_002718"
     CDS             4..759
                     /gene="fabI"
                     /locus_tag="ACG8XC_002718"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP9192362.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(869..1921)
                     /locus_tag="ACG8XC_002719"
     CDS             complement(869..1921)
                     /locus_tag="ACG8XC_002719"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFP9192363.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2047..2967
                     /locus_tag="ACG8XC_002720"
     CDS             2047..2967
                     /locus_tag="ACG8XC_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFP9192364.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          905 a          633 c          596 g          968 t
ORIGIN      
        1 attatgtcat ttctagaagg aaaaaaaatc gtcattatgg gcgtcgctaa caaaaaaagc
       61 attgcttggg gttgtgctga agcaatgttg aaacaagggg cagaaattat ctatacttac
      121 caaaatgaac gaatgaaaaa atcattagtc aaattggtcg gcgaagaagc attcactgta
      181 gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa tgaatgccat cggtgaatat
      241 gctggggaga tccacggcct tgtccatgca gttgcttacg ccaacaaaga agaattgtcc
      301 ggaaatgtgt ctgatatttc cagagacggc tatcaattag ctcaagatat cagcagctac
      361 tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg caccaaacag cgggatcgtt
      421 agcatgactt atctaggttc agaacgcgca atcccgaatt ataacatgat gggtattgcg
      481 aaagcttctc ttgaagctgc tatccgttat ttggcagctg agttctcgcc taaaggaatt
      541 cgggtcaatg gaatctcagc tggggcaatc aaaacattag ctgtcacagg tgttaaagac
      601 tatcaaaaac tgatccagtt atctgaatct cgtacgcctg atcagcaagg tgtgacgatc
      661 gaagaagtcg gtaatgcttg tgctttctta atcagcccac tttccagcgg aatcatcggt
      721 gatattatct acgttgacaa aggtgtccat ctttcataaa cttttgttgc attctttaaa
      781 gtaaaacata taaaaatcag gaaaaaactt tttaaagtct cttcctgatt ttttcttttt
      841 acgttttttt caactcatta ctttgaaatt attccacgac ttcaatccag ccttctggtg
      901 cttctatgtc tccaaattga atacctgtca attcatcata caaccgtttt gttactggtc
      961 ctacttccgt ttcactataa aatacatgaa gattgttatt gtgataaatc ccaccgattg
     1021 gagaaataac agccgctgta ccacatgcac cagcttctgc aaattgatct agatcatcaa
     1081 tgaaaatatc tccttcaatt gcttgcagtc ctagtcgttc ttcagctaac tgcaacaagg
     1141 aatatttcgt gatacttggt aagatagagg gcgattgcgg agtgatgaat tttccgtctt
     1201 ttgtaattcc aaagaaattt gctgcaccta cttcttcgat tttcgtatgt gtggctggat
     1261 caagataaac acaatctgag taatccagtt cttttgcttc tgttcctgga agtaaactcg
     1321 ctgcgtaatt tccgccgact tttgctgcac cagtcccctt aggagcagca cgatcgtaat
     1381 cagatacaat aaaattcgtt ggttttagtc cacctttgaa atacgctcct actggcgtac
     1441 agaagacgac aaataaatat tctggagctg gatggacacc aatgttttca ccgaccccaa
     1501 tcagtaaagg gcgcaagtat aatgtagcgc ctgttccata gggcggcaca aaatcttcat
     1561 ttgcttttac cacttgcttg acagcatcaa cgaacatctt ttcagggacc attggcatca
     1621 gcaatcgtct agcactttga ttcatccgtc gactattttg ttcaggtcga aagagattga
     1681 ccttgccgtc ttttcgacga taagctttta gaccttcaaa acattgctga ccataatgaa
     1741 gcgctggaga gccttcatga atcgtcaggt aattatcttc tgtcaattct ccttcttccc
     1801 attgtccgtc tttccatttg gcaataaaac gccaaggagt tttgatataa gaaaaaccta
     1861 gattgttcca atcgatttgc atatacttac caccttcgtt attttctcca cattttatca
     1921 ttatccatat tatttgtcat tttattttca gcttttttcc ttcattatag ttatttttgc
     1981 tatttccttt tttgaaaact tcttggtaca atacagaaag aatcaaattt tagaaaagag
     2041 gattttatgt tttcattatt agcaactgcc ggtacgacag actcttctgg cgagacattg
     2101 acagatcaag ctgtcagcaa agtaaatgcg tttcagcggt tttgggacgg gattgattgg
     2161 gacgccattt tggctgcctt tatccaaaaa agtttatatc tgattttttt gatcgtcctt
     2221 ttttggattc ttcaccgtgt gggaaaatat ctgattgata agtcttttaa tcagtattct
     2281 aaaaaagcca ttctaaacga gagccgctta aagactttac atagtctttt aaatactatt
     2341 tttcattata cccttggttt cttttttatc tatgcaattt tatctgctat cggtgtgccg
     2401 atcggatcac ttttggctgg tgccggaatt gcaggtgtgg caatcggtct tggtgcccaa
     2461 gggttcatga gtgatgttat taccggattt tttattatca tggaacaaca gatggatgtt
     2521 ggggactaca tcaagctagc aaacttaacg attgaaggaa ccgtcgtatc tgttggtatc
     2581 cgtacccttc agctgaaagc aacggatggt actgtccatt tcatcccgaa tcgaaacatc
     2641 accacgatca gcaatctctc tcgtgcaaat atgcaagtac ttttagatat tcggattgtt
     2701 cctgaagaag gatatgacaa aatctatgag atcattgatc gagtaaatcg tacattagct
     2761 gaaaaatacc aagaagagct ccaaactgaa ccttctatct ttggattagt ggatcttgga
     2821 aacagcaatt ttgcgattcg tacggtctgc tacgcattga acggaaaaca atatgcctta
     2881 aaagaagagt tcttgagctc ctatgtcaaa gaattgacag cttctggttt cacgattccg
     2941 aacagtccaa ttgctgtcgg aaaataattt ggacaaacga tgcaacgaat ctcactaaaa
     3001 agagcgtggg acaaaagtaa agaacacttt tgtcccacgc tctaaacacg tataaacggc
     3061 ggaagcagaa gcaactcctt cggaaataag ctgaaattca ca
//
LOCUS       DAZKCY010000193         2982 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000193 DAZKCY010000000
VERSION     DAZKCY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.198"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACG8XC_002721"
     CDS             <1..189
                     /locus_tag="ACG8XC_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9192365.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="ACG8XC_002722"
     CDS             complement(275..1369)
                     /locus_tag="ACG8XC_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFP9192366.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1558..2319
                     /locus_tag="ACG8XC_002723"
     CDS             1558..2319
                     /locus_tag="ACG8XC_002723"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9192367.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="ACG8XC_002724"
     CDS             complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="ACG8XC_002724"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9192368.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          958 a          585 c          499 g          940 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtattt
     1501 tccctctatt ttgttatcat taaagataac aaaatagtaa attggaaagg aacagttatg
     1561 aagaaaaata aagttatttt aagtattagt ttagcagcag cattgctgtt tgctgctggt
     1621 tgttctgcaa acaacacgac ttcttctagt tcgtctacag aaagttcaaa aagtacatct
     1681 actgcggaaa gcacgactgc cagtagcgtc gatttgaacg cattggaatt accacaattg
     1741 acgaaagatg tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc
     1801 aaaatcaaat tatttcccga acacgcacca aaagcggttg aaaactttat gacacatgca
     1861 aaagacggtt actacaacgg actgactttc catcgcgtca tcaaagattt catgatccaa
     1921 ggtggcgatc ctaaaggcga tggcactgga ggggaaagca tttggggaga aggatttgaa
     1981 acagagatca acaaccacct ttataatata agaggtgcgt tatctatggc tcgctcacaa
     2041 gatcctaaca gtaacggcag ccagttcttt atcgttcaaa atacagatga tatgcatgat
     2101 ggtcttttaa aagacgacta tcctcaagca atcatcgacg cttataaaaa cggcggttat
     2161 ccttcactag atggccatta tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg
     2221 gatgctattg caaatatcga aacagatagt tcagacaaac caaaagaaga agtaaaaatc
     2281 gataaaatca atatactgca agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg
     2341 catagatgac aacgtatgct aggattcagg cgtcttcttt ttacataaaa agcaagtaag
     2401 cttccttttg ttttgctata tcaggtttta tagtcaagca ttttggttga cgatgaacgt
     2461 gaactcgcac tcacagactt ttttatcatc tacatatgct gcagataacg ctgtaccgac
     2521 tgatcccttc attttggtga tttcaaaatg acatttcagc acatctcctg gaacaacttt
     2581 ttgacggaat ttggccttat tgatcccacc gatataagct ctttttccta gaaactcttc
     2641 tgattttagg ataagtatcg aacctgcttg tgccagtgtt tctagaatca acgcacccgg
     2701 catcactgga ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg ttacattttt
     2761 tgtcgcaacg atcttttttc ctggttctaa agtatctaca taatcaatat aacaaatagg
     2821 ataacgatta ggaatcaatt ccattacttc agtagcagat aatacgtaag acataattaa
     2881 actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta tcatattcct
     2941 aaatatttag caatcttttt ttaatttaag aaaaaatagt tt
//
LOCUS       DAZKCY010000194         2976 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000194 DAZKCY010000000
VERSION     DAZKCY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.199"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..908
                     /locus_tag="ACG8XC_002725"
     CDS             141..908
                     /locus_tag="ACG8XC_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9192369.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            898..2940
                     /locus_tag="ACG8XC_002726"
     CDS             898..2940
                     /locus_tag="ACG8XC_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9192370.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT          964 a          481 c          597 g          934 t
ORIGIN      
        1 ttaattttgc tgaataaaat aattgaataa aattttttat tcagtccaag taaaagtgac
       61 aaaaacataa gagcaaatta ttcgtagata tgctactatg caaataaata gaaatgaaaa
      121 atgaaagagg aggagaagaa ttggaaaaga tgatcgaagt gaaacatcta agtaaaagct
      181 acggcagcaa actttctccc gtcaaagcct tagataatat ctcattcaca gtagaagaag
      241 gcgagtttgt tgggattatg ggcccaagtg gtgcaggaaa gacaacgctg atgaatatct
      301 tgtccacgat caatttgcct acgatgggca gtgtctcgat ccaaggaaaa gaaatcacaa
      361 aaatgaaaaa cagtgagctc agtgactttc gtcgaaaaaa actaggattc atttttcaag
      421 agttcaattt gatcgatacc ctaaatgcaa aagacaatat cctattgccc ctagcagttg
      481 aacggatgac taaagaggaa atggaaaaga gagtaagaca tgtagctcaa ttgttgaata
      541 tagaagaact gctcaaacgg tatcccgatg aactttctgt cggacagaga caacgaattg
      601 cagctgctag agcacttgtt gttcagccac aagtgatttt tgcagatgaa ccaactggct
      661 ctttggactc gaagtcggct acagaattgt tgaactactt gaagaccatg aatcaaaaag
      721 aaaaagcaac gatactgtta gtcacccatg atccatatac agcaagctac tgcgatagaa
      781 ttttatttat taaagatggt gtgatttttt ctgaagttgt cagaagaggg acaagaagag
      841 agttctttga gaaagtaata gacatgcagg cgacgattgg cggaggagga aaaatgaatg
      901 ctgtataaac tagccgttaa aagttttttt cggcagtcac gaggctatct tgtttatttt
      961 ttcagtttga ctttatccgt aatgatctat tattccttca gcgcaatgac ctacgatcaa
     1021 tctttagtca gaagagcaag tcaagatact tcgatagatg gtggattgag ttttggtgga
     1081 tttatcatta ctgttgtttt gctttttttc gtattcagtg ccaaccgatt tttcttgaat
     1141 agaaggcaaa aggaaatagg catctatcag ctttttggta tgaataaact gcagatatca
     1201 ggtatatatg tgttagaaat catgataatt ggattgttcg cctgtatttc tgggatatta
     1261 ctcggtatta ttttttccaa actcttttca atgattttgg ttcgaatgat ggatatggat
     1321 cttactagtc cattttttat ctccattcca tcagtagtag atactttatt tgcttttttc
     1381 atcatccttt tagcagtatc tgtatattca atatggaaaa tatggcgcta tccaatgatt
     1441 cgaagttttg gagaaaaaga acaagcagat agttcaacaa tgcgcttcag gacaaggcac
     1501 cgattactcg cagtcatcgg gttactactt ttaacgagcg gatattttgg agctagtcat
     1561 tttagggaaa tcacttcatg gatgatcagt tatcattatc ttgatacatt agttttcttt
     1621 tttccttttt taattttttt cgtttgtgtg gtcggtactt atttgttttt ctgttataca
     1681 tttcggttat ttttgatcat attgagtaaa tggaagattt attactatgg tatcaatttt
     1741 ttgataatgg gtaacacgca agttcatttg ctcaaaagtt ggcgtacaaa ttctctgatt
     1801 acagtcattc tcggaatttc tttaatgaca attggcggca cggctagtat ctctactatt
     1861 ttatcctacc aggagaaagt ttcctccccg atggactatc agtttgatgt tgagacagag
     1921 aaaaaaatca agccgattct agcagaggaa gggcagaaga taactaaaga aatcaccttg
     1981 cattataaag tcattgggag tatctatagt caaaatctcg aagaacctat gtttcaaact
     2041 gctaatctga tcacggaaac acaatatcaa gccttcagaa aagttaatcc agatttgccg
     2101 aacatttctt tgaaatcacc tggtcatact gttttgcttg atcaaatgca aactcttttt
     2161 cggaatattt cattatatgg atctagtatt ggactgccag ataatcaaga attgaagatt
     2221 caacaaatgc ttccaagtgt attaggtgac gaaacaatgg tatatctaag tcctgtactg
     2281 attgtagatg ataccgtttt tgaaaaagca aaaggtgtgg attatcaagt agtcaatgta
     2341 gatgtatcag gtggaaataa tgaaaaaata gatgaacgac tgagcaatga cctgtcgata
     2401 aagtggctca atgctgttta ttattcttat gatatcgtaa atggaagtct acaaggagaa
     2461 attagcacaa agaaacttcc ggatgaccaa atgaaggaag cacaatttag ccaagaaagc
     2521 tctcgtttga atttctcttc cagctattcg aaattgcgtg cagttaatcg gcgaatcggt
     2581 atcaatattt ttgtcacact atttgtcggg atgatcgtga ttattgctac tggtagtatt
     2641 ttgacagtcc ggcaattagc agaagcagag gaagaaaaag aaaattatca cctattgact
     2701 aagctgggga ttccacaaag aaggatcaga cggatgatct tcgagcaaaa cgcacttacc
     2761 ttctttccac ccatgatatt agggatcact cacgcagtat ttgcaatcaa tgtcttcact
     2821 cagtatataa aaactgccga ttactggttg gcttatcttg tgagtggctt attgatcctt
     2881 atctatcttt tgctttatgt cattacgagt cagttatatt gtcggattat cgaggaatga
     2941 tatacggaat aagacaagat aataaataga actaaa
//
LOCUS       DAZKCY010000195         2855 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000195 DAZKCY010000000
VERSION     DAZKCY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.200"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..615
                     /gene="lepB"
                     /locus_tag="ACG8XC_002727"
     CDS             64..615
                     /gene="lepB"
                     /locus_tag="ACG8XC_002727"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9192371.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(698..1240)
                     /locus_tag="ACG8XC_002728"
     CDS             complement(698..1240)
                     /locus_tag="ACG8XC_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP9192372.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1477..1875)
                     /locus_tag="ACG8XC_002729"
     CDS             complement(1477..1875)
                     /locus_tag="ACG8XC_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9192373.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            2147..>2855
                     /locus_tag="ACG8XC_002730"
     CDS             2147..>2855
                     /locus_tag="ACG8XC_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9192374.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISN"
BASE COUNT         1012 a          492 c          489 g          862 t
ORIGIN      
        1 tttttttgat atttttctta taataaagaa agaatgaaaa aaagagggat ggattgatag
       61 agattgacaa aaaaacaacg ctatattaat cgcttctggc ttgtattcaa atacttactt
      121 gtttctgtat tccttgcgtt catgttgcgg ggatttttat tcatcccggt accagtggaa
      181 ggcaattcaa tggaaaacgt gttgaagcaa ggtgatatgg ttgtgatgga aaaattttcg
      241 gagatcagac gatttgatat tgttgttttt caattagcag atggcacgat ttatatcaaa
      301 cgagtaatcg gtcttccagg agaaaatgtc agctaccaaa atgatcaatt gaagatcaat
      361 gggaaagtag ttaaggaacc ttatctaact aaaaacctga aatcagatca tgcaaatgca
      421 tcctatacga cggactttac actacaagag ctaacgggac aatccaagct tccggaagat
      481 aattattttg tacttggaga taatcgaaga gtttcaaaag atagtcgttc gtttggcacg
      541 atcaataaaa ctgatattct aggtaaagca agatttgtct attatccact tgatgaaata
      601 aaatggatcc agtgagaaac aataaagcgt gcagtctgtt gagactgcac gctttattgt
      661 ttaatccaaa atcagtttac taccttagta cgtaaaatca aacagctcgt ttaaacactt
      721 ttctagcctc taaagttttg ataaatgaat aaacaaccat tatttgaata gggatttgga
      781 tcatattcct gatgaatcta cctagaaaaa ttactttgaa atctgcacca aacaaaacag
      841 acatcagcca agaattgata aaaaggctga ctgggatcat aatcaataat tgggcagcga
      901 tgacggaagg caggtttatc tttttatgat ataaaaataa gccattaata aatccaccaa
      961 ggaaagccgt gataagaaaa gcagcttgaa acgttcctga accaaatatc gtgaaattta
     1021 aagtgttcga caacatttca agcaaaccag cccagatcgg tccatataga taacccataa
     1081 gcgccatact taaaaatccg aatgtgattt gaaaataagc aggaccaatc gctatcttgt
     1141 ataagatgac atcaagggct aataaaagag cagaggtcga caacaatctt gcattcattt
     1201 cgggcaatct aaaaacgtct tttgcaatca aagtgttcaa aatattctct cctttgtcag
     1261 ttaataataa aaggatacca taaaatcacc aatgtgtcat gacacattgg tgatttacat
     1321 acactggcat ctaacaatta aaaaaagggt gagaagactg cttttataaa ctgtaaaaaa
     1381 gagattgtac gaataaaaaa aatagaggcg gagaatactt atcgtatcat ccgcctttgt
     1441 ttttttgtta taaaaaacgt cagattagtt atctgcttac atcttcatcc atacgggttc
     1501 taatgtattt ccgtctagat cttggacttc taatgaaaac atttcttctt caggcattcc
     1561 catatctact tcgtggaaag taccaccatt ttcttttgca atttgagcaa atttttttac
     1621 ttcatccgca ttttcaacac taaacgaaat caaagcacca cttgtttttt tattatcagc
     1681 tatttcttta tcttttataa attttttata aaaatcatga gagagaagca ttatccaaaa
     1741 agtgtcatcc cataccattg aaccagcttc ttcagtagaa aattcttcat tttttttaaa
     1801 acccagcttt tcataaaaag caacggaacg attgacatca gcaacaggaa aattaacaaa
     1861 aaccatacta ttcatttgtt tcgtctcctt tctgttttgt tcgtctatca tctcttattt
     1921 aggaaacgct gtcaaaaatt tagattcata tatagaaacc aaaataaaaa tttgcatcat
     1981 caaaaaagtg aagatgtgat agaatgagga ctggttcact ttcacttaac taatagatgg
     2041 aatttcttta taacgccgat tgtaaaatta agctagacaa ctaaaaaagt catttgtgct
     2101 acactcaaaa tagttccgtc caaagaatta taaggagtga gtacaaatga cctataccca
     2161 tcttacaacg gatgaacttg taatgataga gtcttatttc aaaataaatc aatctgttgc
     2221 gaaaactgcg catttcttga atcgttcaag acaaacgatc cataaagtat acctgttctt
     2281 caagcaagga aaatcagcct tagagtacta tcaacagtat aagaaaaaca aatcaaactg
     2341 tggtagacgc ccgcttgttt tacctgagga acaatcagaa tatattcaaa gaaaggttgt
     2401 tcaaggatgg acacccgatg tgattgttgg tcgtgcagcg tttcctattc gttgttctgc
     2461 tcgtaccatt tatcgtatgt tcaaaaaggg gctatttaat ccttctgact taccgatgaa
     2521 aggcaagcgt aaaccaaatg gacatcaaga aagacgtgga aaacaagctt tccgtcgctc
     2581 tattcatgaa cgtgaaaaag attatagcca attctcaaat gagtttggtc accttgaagg
     2641 tgacactatc gtaggtttga aacataaaag tgctgtaatt accttagttg agcgattatc
     2701 aaaagttatc atcacattga aaccgtgtgg tagacaagcg attgatattg aaaacaaatt
     2761 aaatcattgg tttgaatctg taccgaaaaa tctattcaaa tccatcactt ttgattgtgg
     2821 aaaggaattt tcaaattgga aacagatcag taatg
//
LOCUS       DAZKCY010000196         2853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000196 DAZKCY010000000
VERSION     DAZKCY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.201"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            527..>2853
                     /gene="esp"
                     /locus_tag="ACG8XC_002731"
     CDS             527..>2853
                     /gene="esp"
                     /locus_tag="ACG8XC_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFP9192375.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1032 a          438 c          560 g          823 t
ORIGIN      
        1 cattttttgt ttcgctaaaa acgagtgaaa aaatgttgat aactccaatc attcgtttaa
       61 gattctagga atcattcaga aatgctgtga aaccgatact ttataattat aaagaaggtg
      121 atttcaaatt ccgaagaagg aatttgaagt caccttttta cttatgtcac agtctcattc
      181 agtaatgcgg gataaggctc cttattccgt tatttgaata ctctgaagtt taatctaaga
      241 aagggtaata ttttgaaaac atgcttcatc gcatagaaat gaacttaatt tcagaaagaa
      301 tacatacata tctttttata tgttttcagc tgctgaatgg ttaaaaagca caaatatccg
      361 aaaaaaacat gaagaaatgt actttttttg ttgtaatgtt catgaaattc tctaagtaaa
      421 ttagttaaca taaggttagt aagattccta ttctgtgaat aatacgtatc agatttatct
      481 tttattctta ctaggaaagt aattattaag ggagttgatt tgaataatgg ttagcaagaa
      541 taataagaga gtatttctag aaaaaacaaa gaaacgagta ttgaagtaca gtatcaaaaa
      601 attaagtgtt ggggttgcat ctgttttgat tggtgttggt cttgtcttgg gaacaactga
      661 actagttaaa gcacaagatg aaatttctcc aagcagccca cttgaaacag cgataagctc
      721 cgtacaagta ggtgacaaag tagcttcggg gaatactttt caggagaatc ctggttatac
      781 gaaaaattat aacttttcgg atttacaatt tagtcctcaa gaactaactg gagacacact
      841 aaaaggcaat acaattggat ttgaagttta cggaaaacat aatattgcag catcaactaa
      901 aaactgggaa atccgtcttc aattagatga acgattagct aaatatgtag aaaaaattca
      961 ggtcgatcca aagaaaggaa ttggatcttc tagacggacc tttgtaagaa ttaatgattc
     1021 gctgggtaga cctacaaata tttggaaggt caattatatt cgtgcaagtg atggattatt
     1081 tgctggggca gaaacaaccg atacgcaaac cgctcctaat ggggtaatta cgtttgaaaa
     1141 gagcctagat gaaattttta aagagatagg catagacaat ctcaagaccg atcgtttaat
     1201 gtatcgaatc tacttggtaa gtcatcaaga cgatgataaa attgtaccgg gtattgatag
     1261 tacgggctac tttttaacag attcagatga tttctataat agcttagatg tatctgaaaa
     1321 taatcctgac cagtttaaac atggatcagt aagtgcaaaa tatgagaaac ctaatactca
     1381 aacaaaagat ggttcaggat ccacaggtgc taatggagct ataatcttag atcataagtt
     1441 aacgaaaaat tataatttct catattcatc ttccgcaaag ggcacgcctt ggtatgctaa
     1501 ttacaaaatt gatgaacgat tagttccata tgtagcgggt atacagatgc acatggttca
     1561 ggctgacaaa gtaacatatg atgtttcgtt tgaatccgga acaaaagtag ctgatttagc
     1621 aatcgagcga cgggaaggtc atgaaaatta tggcataggt tcaataacta ataatgattt
     1681 aactaaactt atcgattttg ctaatgcaag tccacgtcca gtcgttattc gatatgtttt
     1741 acaattgact aagccattag atgaaatctt agaagatatg aaagcaacag cacaagttga
     1801 agaaaataaa ccatttggtg aagatttcat ctttgattct tggttgtcgg atacgaataa
     1861 aaaattaatc cagaacactt atggaacagg ttattattat ttgcaagata ttgatggtga
     1921 tggaaaccct gacgataaag aagagagcgg agacacgaat ccatatatcg ggaaacctga
     1981 attagaagaa gtatatgatg ttgacacaac agttaagggg aaagtattca tccacgagtt
     2041 agcgggaaca ggtcacaaag cccaacttgt tgataaagaa ggtactgtat tagcagaaaa
     2101 aactatcgct ccaaatgaaa aagatggggc tccaatttca gatactgtag aatttgaatt
     2161 tacgggtgta gattcaagta aattaatcgc gaaagatgaa ttaaaaatcc aaatcgtttc
     2221 tccaggtttt gataaaccag aagaaggttc aaccgttatt aaggaatcac caaaagcggt
     2281 tgataaacaa accgtggtag ttggatttaa accagatgct aaagaatcaa ttcggaataa
     2341 taaaaactta cctgaagatg cagagtattc atggaaaaca gagcctgata cttctaacgt
     2401 tactgatagt acgaaaggta ttgtaactgt taagatcgga aatcgaactt tcgacgtgga
     2461 tgtagagttt gctgtaaaag cttctcaagc tatggaaaat gatgcaacat acgtacctat
     2521 aacaacaact ccagaaacga cagttcaaag tggaaaacct acatttgata aaccagatgt
     2581 tcctttagct aaagatgcct tttcaatttc agatgtctat aataaggact tcggtaatgc
     2641 aagtgttgat gcaaacactg gtattgttac attcactcca gctaaaggtg taggagaatc
     2701 ggagccgatt actggaacaa ttcctattaa aattgtttac caagatggtt ctgtaggcac
     2761 gaccgattta gcagtaactg taagtaaaga tatttatgaa aatccaggag aaaacattcc
     2821 tgcaggctac cacaaagtaa ccttcaccgc agg
//
LOCUS       DAZKCY010000197         2846 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000197 DAZKCY010000000
VERSION     DAZKCY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.202"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..1101
                     /locus_tag="ACG8XC_002732"
     CDS             316..1101
                     /locus_tag="ACG8XC_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9192376.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1114..1965
                     /locus_tag="ACG8XC_002733"
     CDS             1114..1965
                     /locus_tag="ACG8XC_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP9192377.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2089..2541)
                     /locus_tag="ACG8XC_002734"
     CDS             complement(2089..2541)
                     /locus_tag="ACG8XC_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9192378.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLMQTDISLVS"
BASE COUNT          910 a          471 c          576 g          889 t
ORIGIN      
        1 gctgtgacaa aagtcttgtc acagcctttt tttccaaata aatggttttt cagaagtagc
       61 ttcttcggaa ataagccgaa atttcacaaa aatttgagaa gtaatttttg taaattactt
      121 cttatttctt gaagctggcc acttctgtca caacctcttt ctttatagaa ataaaaggac
      181 actagcaatt catgaaaacg attgctagag cgtatcattt gctttagact atatatcttg
      241 gtgtacactc actttaagac ggaaataaaa gttatccgta ataacaggtt taggtatgaa
      301 aaaggagtgg gtactatgta taaggaatta gaaaacaaag tcgcagtagt aactggtggc
      361 tcaaagggga ttggaacagc gattgctcaa cgattcggga aagaaaaaat gaatgtagtt
      421 gtcaactata actcggataa agaaggagca gaaaaagcag tcaaagcaat cgaagaggca
      481 ggcggaaaag cagtctctgt acaagcagat gtcagttctg aagaaggaat acagcgttta
      541 cttgatacgg ctttagaaaa ttttgggaca ctggacgttt ggattaataa tgctgggatg
      601 gaaaatcagc atccaaccca tgaacttagc cttgaagatt gggaaagagt attaaaagtc
      661 aatttgactg gcgtttttat cggaacgaaa gcggctttaa attactttac agagaatgat
      721 aaaaaaggaa atatcatcaa tatgtcttct gttcatgaac aaatcccatg gccaacgttt
      781 gctcattacg ctgcttcaaa aggcggagta aaactattca ccgaaacggt agcaatggag
      841 tacgcaccaa agggaatcag aatcaatagt ataggacctg gtgctatccg tacacctatt
      901 aatgcggcaa aatttgatga tcctgaacaa aaagaactct tggaaagtat gatcccacta
      961 ggacgcgtag gtgaaccaaa agaaatagcg gcgacagctg catggttggc ttcagacgaa
     1021 tccagttatg ttacaggtat tacactattt gcagatggtg ggatgacttt atatccttct
     1081 ttccaaggtg gaaaaggata agggtgaata aaaatgggtt tattgattgc attgatcccg
     1141 gcagttgggt gggggatcca gccactgatt ttgaaaaaga ttggtggcag accaacaaat
     1201 gagatattag gaactggtat tggagccgtg ctcgttggct tacttgttca attattcgct
     1261 tctccagctg gtatttcagt gactacattt ttgattagtt tactttctgg tgcattctgg
     1321 gtcatcggac aaacaggaca gtatacatct ttcaacttga ttggtgtttc taaaacgatg
     1381 ccaatttcga ctgcattaca gttagtcgga acctcattga ttggtgtctt cgcttttgga
     1441 gaatggtctg gaagtatggg aaaaatcatt ggtgcagcag caatcattct tcttgtattt
     1501 ggttctgctt taacggcaat cagtgatgat cctggaaaaa aaggcggtat cacaaaagga
     1561 atcagtctat tagcatcaac aagtattggt tattgggttt acagtgcatt gcccaaacta
     1621 gttgatgcaa gcggactttc cattttcttt ccgcaaatga tcggtgtctt tttaggggca
     1681 gtgatttatg tgattttccg aaaaccaacc gcattttctg aagacaagag ttggaaagca
     1741 acaatagttg gagccatctt cagtgtgtca gcattggctt atattttttc agcaaatgaa
     1801 aatggggttg ctactgcata tatcattaca cagctaaacg tagttatctc tacattaggc
     1861 ggtatgtaca tcctgcatga aaataagaca ccaaaagaaa aaaaattaac gattatcgga
     1921 ttattgctga tcgtagtagg aagcatggtc acggtcttta tttgaggata aagatggtaa
     1981 aatctcattt attaaaaaaa tatttgtttg aaccaaacag taagtactta taaaaaaata
     2041 gcgctagaat ttccaacatc tctggaattt ctagcgcttt ttaattgttt aagatacaag
     2101 tgaaatatct gtctgcatca ataatttttc attgatccat tgactaaagt ttacgacata
     2161 attgaagtta ccaccgatta gtatctccgc atgattgtct ttataaagat cgtattgtaa
     2221 aagacggtct tttttatttg cgtaaacatc gttatggtac tggactaatt gttctcggaa
     2281 ttgattgatt gctgtaaaat cacgatactg aaggttgaaa cgcatcgtga ttggtgcatt
     2341 tagcatgaat cgctgcattt cttttgctgc ttcctctgct tgatctccgt ataggaactc
     2401 aaaaagatta cgttcagaaa atttccattg cccgccaacc tttgttcctt ttagttttcc
     2461 ttcatgaaga taatttctga tagaacgtgt agtaacacct aattgttcgg ctaattcatt
     2521 tactgagtaa aaatctttca atgttcaccc tcctatgtga atggttctac tgacttagtt
     2581 gatatttgct taatcacgaa atgtagaaag aacgatttta attaaggtca ttgtaaaaat
     2641 caagtcagaa aattctaact tttctttctc taaagaaatt cgttctctcc taaagtgata
     2701 ttatatttaa atatacctga aaataatggg cagtattatg aaagatcact gatattttct
     2761 cgtatttttt ccttattcaa aagttttgca ggaaaattgc tgaaaaaata attttactaa
     2821 gtggaaaatt caaagccatt caatca
//
LOCUS       DAZKCY010000198         2742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000198 DAZKCY010000000
VERSION     DAZKCY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.203"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            483..569
                     /locus_tag="ACG8XC_002735"
     CDS             483..569
                     /locus_tag="ACG8XC_002735"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFP9192379.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(871..1095)
                     /locus_tag="ACG8XC_002736"
     CDS             complement(871..1095)
                     /locus_tag="ACG8XC_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192380.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1461..1745
                     /locus_tag="ACG8XC_002737"
     CDS             1461..1745
                     /locus_tag="ACG8XC_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192381.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1748..2164
                     /locus_tag="ACG8XC_002738"
     CDS             1748..2164
                     /locus_tag="ACG8XC_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192382.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2301..>2742)
                     /locus_tag="ACG8XC_002739"
     CDS             complement(2301..>2742)
                     /locus_tag="ACG8XC_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9192383.1"
                     /translation="FVLFTGFEINKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFT
                     SLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELK
                     KYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKISLRNWLEE"
BASE COUNT          907 a          515 c          440 g          880 t
ORIGIN      
        1 cttctgtatc cgttgggaga gtaaggctca actgccccct aaaaagggtt ctaaaaaatc
       61 tttaaaaaat aaataaaata aacgcgcgcg cgcgagcaaa atttcccgcc aaaaaatcac
      121 ccacttttta aaataaacag gttcgcgcat gatattaaac tgccaatcta gctccctatg
      181 gtcgctgtaa gaaggagtga tcgctacgct ctcacatttt acttgcagca gtcgccttta
      241 ggctccttcg ccaaaaataa ttcattaggg actcctttgt ggcgtcctga gagctcgtct
      301 aagccgttct aagacaagat tcgataaaaa ctatgtcctt cgttctaaag cgccttacag
      361 gcggtcaggg caagccctaa caaccccaag gcaataaaaa acttaggctt atttttgctt
      421 ttttattgta taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt
      481 tcgtgttcac taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt
      541 actggttgaa taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa
      601 aagccccaga gaatccgacc tctctggggc tttcgtgtgt ttcgtgttca cttatttgca
      661 atcaaattat aacagaaata aatatttatt tcaaaaatat cctacaaaag tctaaaatcc
      721 gtaattattt cttatatgca acatttacaa ataaagtcgc tctgagagct cgtatttttc
      781 gatttaagac gttttattta gtttttggtg tatttcattg gtccaagcta aaaaactctt
      841 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
      901 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
      961 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     1021 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     1081 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     1141 tctttgccgt ctatactctc aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc
     1201 gtgcattttt aaatttggtt atcgattaca cgtgcagcac tcccccccga attttaaaga
     1261 gcataaggaa acgtgtaccc actttctaaa aaaagtgggt agataggctg tgcctatcac
     1321 gtttctcaga aaaaaatttg atatcacttt ttcgccatcg tgggtagctt atgtatagca
     1381 ttttggcaaa aaacagctat acaaatatag tatttgtatg gtatacataa attatacata
     1441 attgcaaaag gagatttttt atggacattt tagttcggaa tattgatcca aagtttgttg
     1501 caacaataga caaacgttgc caagaactca cgaataaaac aggagaaaaa tggagtcgaa
     1561 ataaatatct aaaagtttta atcgaaagtg attttgaccg tcaacttacg acttacaaaa
     1621 aagaccaatt cgatttattg ctcgataaat ttattgctat ccaaacagaa aatactcaaa
     1681 tattaagcga gtatgtctac acaaataatc aaatcattga aatgcttatc ggaggaatga
     1741 tataaccatg agaaaaactt ctgtccaaaa acatgttact ttgtacccta gtcatgttga
     1801 cgttttagat tccatcatcg aaaaaaatcc agatatcaat aactactcgg aagccattcg
     1861 ctttttatgt atgagttatg aacccgatac caacacgacc actaccagca atcaggcaaa
     1921 actaaatgct atgggaaaag aaatttccat catgactgaa atcatcgcag aatttgctga
     1981 tttatatcta aaagatacag gcgttcttat tggcacaaat tctaaagtat atcaagaagt
     2041 gaaagaaaaa atcgaagcta aactaaatcc atcaaatcga aatcgaccaa agaatcatca
     2101 aaaagaaacc agtcaaacag tcacttctag aaatttcgac ccaacaaatt ttctaaaacg
     2161 ataaaaaaca gataggagtt attcctatct gttttttatt tagaagcccc atacaatatg
     2221 ctagaatgta ctagcaatct tagtcggttg taagggactt gtctacttgc ttaatgtaag
     2281 gggggcgagt tcttttttat ttattcttct aaccagttac gcagagaaat ctttggcaaa
     2341 tctgacgtta ggcttgaaaa agtaaattct aatttggcga ttttattgcc cttttttgct
     2401 tttatttttg tgactttaag atcattgaag tattttttta gttctcgtaa tgcaggattt
     2461 aatacttttt gatcaatgtt acccatttga tagtattctg ggatatccat taagctccta
     2521 aactcttcaa tacgaactac ataataacca gtagaacggt actgcataag cagtcggaat
     2581 agagtttttg catacgtaga acgcaacgac gtgaaagcag ataattcaaa tctactaaat
     2641 tcagctgtta aaccattaat aatatattct aggtccggat taacactgac ttcaacaaac
     2701 ttttttgttt tattgatttc aaaaccagta aacaaaacaa ag
//
LOCUS       DAZKCY010000199         2670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000199 DAZKCY010000000
VERSION     DAZKCY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.204"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..235
                     /locus_tag="ACG8XC_002740"
     CDS             <1..235
                     /locus_tag="ACG8XC_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HFP9192384.1"
                     /translation="NSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFH
                     QAMKHAKVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            405..665
                     /locus_tag="ACG8XC_002741"
                     /pseudo
     CDS             405..665
                     /locus_tag="ACG8XC_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            828..1493
                     /locus_tag="ACG8XC_002742"
                     /pseudo
     CDS             828..1493
                     /locus_tag="ACG8XC_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            1529..1774
                     /locus_tag="ACG8XC_002743"
     CDS             1529..1774
                     /locus_tag="ACG8XC_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9192385.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1877..>2670
                     /locus_tag="ACG8XC_002744"
     CDS             1877..>2670
                     /locus_tag="ACG8XC_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP9192386.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLY"
BASE COUNT          943 a          394 c          513 g          820 t
ORIGIN      
        1 aaacagtgtc ttaataaaac ttacccgcca taccacagat gttccagata aatattggaa
       61 gctatatacg tactttgttt caaaatgggt caatcgagaa tatcgtcaac tgtttactaa
      121 aaatcagttt catcaagcaa tgaaacacgc caaagtaaac aatttaagta ccgttactta
      181 tgagcaagta ttgtctattt ttaatagtta tctattattt aacgggagga aataattcta
      241 tgagtcgctt ttttaaattt ggaaagttac acgttactaa agggaatgga gataaattat
      301 tagatatact actgacagct tccaagaagc taaagaggtc cctagcgcct acggggaatt
      361 tgtatcgata aggggtacaa attcccacta agcgctcggg accccttgta ggaaaatgtc
      421 ctaagtgtgg caacaatatt gtattaaaaa aatcgtttta tggttgttca aattatcctg
      481 aatgtaagtt tactttagct gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa
      541 aagaattact ggagggaaaa gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc
      601 cctacaatgc cgttgtaaaa attggggaaa agggatatat tgattttatc tctttttcaa
      661 aataaacatg gggctgaccc aaaagtagct cctatatgaa aaatagaggt ctttccattt
      721 agatagtagt ggaaagacct ctatttatct tttgaaagtg aatacaaaaa taaagcacac
      781 tctgctataa tataggtgat taatccattt gagagaggtg ctttatcatg tatcaaaatt
      841 ataccataaa ccaactttgt ttgcctatag atcttgaaat taaattagaa gaaaatgatt
      901 ttgctcatgc cattgtgcag tttgttgact ctattcccga tgaagtcttt cttccttatt
      961 atcaatctat gggtagaccc caatatcatc ctcgtatgct actttccatt attctttgtg
     1021 cctatattca aggggtttat tcgggccgcc aaatacaaaa tatgctgata gatagtattc
     1081 ggatgcgcta cttatctcaa gagcaattcc ctaactttcg tactattaat cgttttcgcg
     1141 ttcatccgat tatgaacgat attctagatc attcatttgt tcaatttcgt gagctactgg
     1201 ttcaaagtgg cttaatcagt ggcagtgctc tatacattga tggtacaaaa attgaagcgg
     1261 atgcgaataa atactctttc gtttggaaaa aaagtatttt aaagtataag gatagccttg
     1321 atcaaaaagc ccttgaaaac taccaacaaa tggtggaaac aaaaatctta cctgagttaa
     1381 tagatgattt aaaggatgaa ctttctatag aagagattga aaggattcgt caatctttgg
     1441 aagaaaagga aaatcaatta attgaagcga ttgaccaaac cgaaacagta gaagggggct
     1501 gacccattat taataaggag tggacaacat gattgatgta aatgagctat tggaagaagc
     1561 tattagagaa actgaaaatc taaatgatgg cgaagttttt cttgttaaag atttattcaa
     1621 gggatatgtg gggaatagga tacccagaaa ggatcgactt ctgcttggaa cgttattttt
     1681 aaaccatgtt agcaaaatga atggtaattt aaaggctatt gaaaagactt catctaatca
     1741 gcagaggtat aaaaagacaa ttgataagtt ttaactatct tttgtgaaat taaaaaaatt
     1801 ataaatatgt tgagtagaag caaaagattt ggcttggaat ttttacagca gtgaatcgat
     1861 aaaaggagca tgcttgatgg tagataatat tattaaatca gtagcagaga aattatcctc
     1921 tctgtcttat atagaaggta ttgttttagg tggttcacgt gcaaggggca cccatacaga
     1981 ggattcggat atagatatcg gcatctatta caattcagaa tcatttgaca taaatactat
     2041 taatcaattc gctacaaagc tggatgatga gcatagaaat aaccttgttg tacctcccgg
     2101 agcatggggt gattggatta atggcggcgg atggttagtc ataaacgggt atcatgtgga
     2161 tttaatttta cgtgatatta aacgtgtgga acaaataatg aaagatacag agcacggaat
     2221 tgttactgcc aattatcaga ctgggcatcc ccatggttat attagtgcaa tgtatcgagg
     2281 agaattagcg attagcaaaa tactatatgc taagaatgaa agcttatgcg aattaaaaaa
     2341 acaggcagaa acttatccca atgctttgca gaaaagttta gttaactttt ttatgtttga
     2401 agcagggttc tctttaatgt ttgtaaaagc aaattcggga acagacgata aatattatat
     2461 tgcgggtcat gtttttcgta tagtttcatg tttaaatcaa gtgttatttg catgtaataa
     2521 tgcttattgt atcaacgaaa agaaagctat aaaactgctt gaaacttttg aacataaacc
     2581 tgaaaaatat accgagaagg taaatcatat ttttgaagta ctcggtatct cactttttga
     2641 atgctacgac atgaccgaga agctttataa
//
LOCUS       DAZKCY010000200         2664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000200 DAZKCY010000000
VERSION     DAZKCY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.205"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..962)
                     /locus_tag="ACG8XC_002745"
     CDS             complement(<1..962)
                     /locus_tag="ACG8XC_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9192387.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
     gene            1134..1268
                     /locus_tag="ACG8XC_002746"
     CDS             1134..1268
                     /locus_tag="ACG8XC_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192388.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(1261..1896)
                     /locus_tag="ACG8XC_002747"
     CDS             complement(1261..1896)
                     /locus_tag="ACG8XC_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192389.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(2009..2644)
                     /locus_tag="ACG8XC_002748"
     CDS             complement(2009..2644)
                     /locus_tag="ACG8XC_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFP9192390.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT          762 a          449 c          399 g         1054 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagtg
      361 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgacgc tctaatatgg ttattaaact taataacggt agattttaca
      601 tggtctttag catacttatt taaaaagcgt tgatatccca ctctatctaa atcagaaatc
      661 aataccgctg gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg
      721 ataggagata ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat
      781 aatagatttt tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg
      841 gcatcttttg cttctccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg
      901 tcatcttttt tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc
      961 ataattgaat cagctctctt tctctgatac aataggaact ataaagaagc ccattgtttg
     1021 ggttttatta ttttttgcac atcctttttg cttgccggcg caaggatgtg ctatttgtcg
     1081 ttttaatggt ataattggat gtgtattaga tcatccaatc agaggtgttt attatgatta
     1141 aagtgatttt taaaagaata actgaacttc cagagtttag attatttgtt gattattggt
     1201 atgtctggtt ttttattgtt gcattgctga taggtctaga tgtcatatta gaaagaaaac
     1261 ttaagtaaga gttgagttaa taaattgaat tcctaaatga gatattttgt agcccatatt
     1321 ttgtttgccg acatcaccta atactatgga gctaatcgtt ttttctaaca gaccaaaatc
     1381 agataatcta tttaagctac catctattag ctcttgtgaa tagccagaca tgctgatatt
     1441 tccagtgaaa ttttcgtttc cacgactcat taagcatgct aacaattgaa tctcaatcga
     1501 tgttaattga ttcagtacaa gaagataata ctcctcgtta tcccatgaag cgttgttaat
     1561 tcgaagtaaa caattcttat aaagattttt atacatatct attttatttt gtgcctttga
     1621 tttttctacc tcgtcattta tcccttcgaa tattccaatg agttggtcct tgttctctaa
     1681 gttgaaaaca tcttctggaa tttgatcgcg aatgttttct aatgattcgt ttaaattttt
     1741 gtagaatttc tcgatgcgtt tgaaacgctt ctcgttttta gacccaaaat aaagtgactg
     1801 cacaacgcca ccaatataag gaattgctcc aataccgcct tcaactatta attcaagata
     1861 ttcttttttg gttagttttt cattgctatt atccatagtt acctcctatt ttgtcatttc
     1921 ctttacaagc attgtgactc ctgatggcac tcttttgtat aggttttatt attgtgcaca
     1981 atctaactct tagtggggcg tggttcattt attttttact tactttacta ttaatcatat
     2041 cccattgagc ataggatttt tctatgttcg atcctcttga actaatttct cctgtaaaag
     2101 ttactttatc gccctttgta aaggtatcag cattgtttaa gtccttcgca aaaattacat
     2161 atgaaacttt aggtgttgca gaaatatcat tccaactttt atcatcgtac tctttgtctg
     2221 ctataatggc tattctagta gtcattgatt cgataacagt tccttcaaaa gtatatgttt
     2281 taccactaat atatttagta aaagctgtat tttgctcagt agctggagta atagaataat
     2341 aactatctaa gaaagcaccc cagtcatgat tatttgtctc taaaaattct tcaaattgag
     2401 ttaggctttt attgctgctg ttagatgtag tagtttgagt atcagatgat gagctttcct
     2461 gtttgttatt gtcatttgcc tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa
     2521 taatcccgat aataaggaga atcataggtg ttttcttatt tgtttttttg ataaatctta
     2581 caattaacat gattaaacct ataataaata aaacaatacc agtaaaacca aatataccgt
     2641 ccattaatag atcctctttc tctt
//
LOCUS       DAZKCY010000201         2655 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000201 DAZKCY010000000
VERSION     DAZKCY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.206"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..259
                     /locus_tag="ACG8XC_002749"
     CDS             17..259
                     /locus_tag="ACG8XC_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFP9192391.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYESLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            275..472
                     /locus_tag="ACG8XC_002750"
     CDS             275..472
                     /locus_tag="ACG8XC_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFP9192392.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            483..1760
                     /locus_tag="ACG8XC_002751"
     CDS             483..1760
                     /locus_tag="ACG8XC_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9192393.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAAKNDQGVDWSIYQGENGRF
                     GYAHDKFAIAQIGGYNGAGLYDQWTYSTQVASAIAQGKRAHTYIWWDVWGSPAIAKQT
                     MDYFLPKIQTPKGSIVAIDFEGGASSNKQANTDAILYGMRRIKAAGYTPMLYSGKPFL
                     LANAYYQQVIKEFPDSLWISAYPDYNVTPTPNWNIFPSLDGIGIYQFTSTYITGGLDG
                     NIDLTGITDNGYTGSDKPATDTPATDAGEEANDTPKSEIKVGDTVKVNFSAKNWATGE
                     AIPQWVKGESYKVQQVNGNKVLLANILSWIDKSNVEILPDSTTVPDKPSAGIQTHVVQ
                     YGETLSSIATKYGTTYQALASLNGLSNPNMIYAGQVLKTNGTANVTRTYTVRSGDNLS
                     SIASKLGTTYQALAQRNGLSNPNLIYPGQVLSY"
     gene            2015..2398
                     /locus_tag="ACG8XC_002752"
     CDS             2015..2398
                     /locus_tag="ACG8XC_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192394.1"
                     /translation="MLKFDEYPDSCPPGNAIKFEGTFYRLCKGNPFCKEDFLTHFEAG
                     FNFPSSKLCEAMALSFYDSYAHAESLKNQYRKKFKDHSIIPVKIIEKYGVGILEEKKG
                     HLNLWENRNVDIFSDLIKEGGDKDG"
     gene            2391..>2655
                     /locus_tag="ACG8XC_002753"
     CDS             2391..>2655
                     /locus_tag="ACG8XC_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192395.1"
                     /translation="MDNQFFSSLEYIFIFDYYDSPLFFISQSSENNRYYLFYSIDDDA
                     YFFSELSASDINYLFMNPNGYDILHYLKDKSKLKFVLISKKSFN"
BASE COUNT          894 a          403 c          526 g          832 t
ORIGIN      
        1 aaaggaggct agttggttga aagatgaagc aatacaagac gtggtagaac gtttagtgcg
       61 tattgaaacg aaactggata attacgaatc attacgcgaa aaagcggaaa gtgcaaaaga
      121 tagagcggat caggcatatt ctattgcgct taataatgca gaagatatca aagaaatgaa
      181 agccaataat aaatggtcgt ggggttacat gattggttta ggcattacga tcattggcta
      241 tttcttgact aaattgtaaa ggagttaaga agaaatgatt ttacccgata agtattatca
      301 agtcattaaa tggacggttt taacagtttt accagctgca tctgttttag tagccacgtt
      361 aggaaaagcg tatggatgga atggaacaga tatgacagta ctcactatca atgcagtagc
      421 aacattttta ggcgttatca ctggtgtgtc ggcttataat ttgaaaaaat aggaggaaac
      481 aaatgaaaaa gaaaattact attactgcga tgagcctatt aacggctctt tttttattgc
      541 caattaatgg atttgctgca aaaaatgatc agggtgtgga ttggtcgatt tatcaagggg
      601 agaacggaag atttgggtac gctcatgata aatttgctat tgcacaaatt ggtggttata
      661 acggagctgg tttatatgac caatggacct actccacgca agttgcttct gcaattgcgc
      721 aaggcaaacg agcgcatacc tatatttggt gggatgtgtg gggttcccca gcaattgcta
      781 aacaaacgat ggattatttc ttgccaaaga ttcaaacgcc taaaggttca attgtagcga
      841 ttgatttcga gggtggggca tcttctaata agcaagctaa tacggatgcc attctttatg
      901 gtatgcggcg aattaaagca gcaggctata ctccaatgct ttattcagga aagccgtttt
      961 tattagctaa tgcttattat cagcaagtaa tcaaagagtt tccagattcg ctatggatct
     1021 ctgcttatcc tgattataat gtaacgccaa ctcctaactg gaatattttt ccatcgctgg
     1081 acggtatcgg tatctatcaa tttacctcaa cttatattac tggtggatta gatggtaata
     1141 tcgatttaac tggtattaca gacaatggtt atacaggttc agacaaacca gcaacagaca
     1201 ctccagcaac ggatgcaggg gaagaagcta atgatacgcc aaaatcagaa attaaagtgg
     1261 gcgacactgt gaaagtgaat ttctcagcga aaaactgggc aactggagaa gcgatcccac
     1321 aatgggtaaa aggagaaagc tacaaggttc aacaggtaaa tggcaataaa gttttacttg
     1381 caaatatttt atcttggatt gataaatcaa atgtagaaat cttgccagat tctacaactg
     1441 ttccggataa accatcagct ggtatccaaa ctcatgtcgt tcaatatgga gaaacactat
     1501 cttcaattgc tacaaaatat ggaacaacgt atcaagcact tgcttcgtta aatggactaa
     1561 gcaatccgaa tatgatctat gctggacaag ttctaaaaac gaacggaaca gcaaatgtta
     1621 ctagaacata cactgttcga tcaggcgata atttatcatc aatcgcatct aagttaggca
     1681 caacgtatca agcgctggca cagcgcaatg gattatcaaa tcctaacttg atttatccag
     1741 gtcaagtact atcatattaa aaaaagcccc tcgatgaggg acggtacttg aaatcatata
     1801 taacattttt gttacaaaat taatgttatg aatgtcacaa agtgttgttt taatatcata
     1861 aacgtttaac ttgaacttat gttccctgta tgctaaagta atttcataat cctcaaagat
     1921 tatatctgct aagaagccca gaggctattg tctttagggc tttcttttgg tttaattgtg
     1981 ttagcagata taaaacgaaa agtggggata tatattgtta aaatttgatg aatatcctga
     2041 tagttgccct cctggaaatg ctatcaagtt tgaaggaaca ttttatagac tatgtaaagg
     2101 taaccctttt tgcaaagaag attttctaac acattttgaa gcgggtttta attttccgag
     2161 tagtaaatta tgtgaagcta tggctctatc tttttatgat agctatgctc atgcagagtc
     2221 tcttaaaaat caatatagaa aaaagtttaa agatcattca attatacccg ttaaaataat
     2281 tgaaaagtat ggagttggta ttttagaaga aaaaaaaggt catttgaatc tttgggagaa
     2341 caggaatgta gatatttttt ctgatttgat aaaggaaggt ggagataaag atggataatc
     2401 aatttttttc tagtttggaa tatattttta tttttgatta ttatgattct cctttgtttt
     2461 ttatttctca aagttcagaa aataatagat actatttatt ttactcaata gatgatgatg
     2521 cttatttttt tagcgagtta tctgcttctg acattaatta tttatttatg aatccgaatg
     2581 gttatgacat tcttcattac cttaaagata aatcaaaact gaaatttgtt ttgataagta
     2641 aaaaatcttt taata
//
LOCUS       DAZKCY010000202         2642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000202 DAZKCY010000000
VERSION     DAZKCY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.207"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1389)
                     /locus_tag="ACG8XC_002754"
     CDS             complement(<1..1389)
                     /locus_tag="ACG8XC_002754"
                     /inference="COORDINATES: protein motif:HMM:NF014437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9192396.1"
                     /translation="MSNKEEAKSIINFVGGKENIAALTHCVTRLRFALRDDTKVDKAG
                     LENLEDVKGTFNANGQFQVVIGPKVADIYEEVISQSGLSGANKEDVKKVAAELNLNWF
                     QRAVKILGDVFVPILPAIVTAGLLMGLNNLLTNPGIFFEQSLIEVYPNIADVADMINL
                     IANTSFQFLPALITWSTVKYFKGNPLLGIVLGLVFVHPDLMNAWNYGQAVTTGDIPHW
                     TIFGLSISKIGLQAQVLPALVSGYVLAKTELFLKKRVPETIQLLVVAPVAILFTSVIT
                     FIIIAPITIAIGNTIVAGIVWLFQKVPFIGGFAYGIGLAPLVVTGMHHAFLPVHIQLM
                     ANGGDYIWPIGVMSSLAQGSAAIAMNFLIQNKKLKGVGLAAGLSAYMGITEPAMFGVN
                     LKYKFPFVVSILTSGIIGGFMASQHVIATSVGISGIPGFLSIPFEFWPIFFICMLASC
                     VVSFGATYLYGKN"
     gene            complement(1382..1750)
                     /locus_tag="ACG8XC_002755"
     CDS             complement(1382..1750)
                     /locus_tag="ACG8XC_002755"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HFP9192397.1"
                     /translation="MSYTTFSMSPISIKEKGKNCLKVTTTVSNKGSRDGYEVVQLYIG
                     AQYGRYIRPTKDLVAYKKVFVKSGDSVTVSFELTNKDFKYYDEEFNEIIDDGAYWIMV
                     GNDSSQALTNKEEIEVRINE"
     gene            complement(1754..1993)
                     /locus_tag="ACG8XC_002756"
                     /pseudo
     CDS             complement(1754..1993)
                     /locus_tag="ACG8XC_002756"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
BASE COUNT          880 a          547 c          404 g          811 t
ORIGIN      
        1 atttttcccg taaaggtaag ttgctccaaa tgagacgaca caacttgcta gcatacaaat
       61 aaagaatatt ggccagaatt caaacggaat agacaagaaa cctggaattc cactgatacc
      121 aacagaagtt gcaatgacgt gttgtgacgc cataaatcct ccaattattc cagaggtcag
      181 aatggaaacc acgaatggga atttatactt tagattaacc ccgaacattg ctggctcagt
      241 gatacccata taagcagaca aaccggcagc cagtccaaca cctttcaact ttttattttg
      301 gattaagaaa ttcattgcaa tagcagcaga accttgtgcc aaacttgaca tgactccaat
      361 aggccaaata taatctccac cgttcgccat caattgaata tgaactggta aaaacgcatg
      421 atgcatccct gtcacgacta atggcgcaag accaatacca taagcaaatc cgccaataaa
      481 tggaactttt tggaataacc atacaatacc tgcgacaatc gtattaccaa tagcaattgt
      541 aatcggcgct ataataataa atgttataac actagtaaaa agaattgcaa ctggtgcaac
      601 aaccaacagt tggattgttt caggcacacg ctttttcaag aatagctctg ttttagctaa
      661 cacatagccg gaaactagag ctggcaatac ttgtgcttgt agtcctatct tagagatgga
      721 caatccaaag attgtccaat gtggaatatc tcccgtggtt actgcttgcc cgtaattcca
      781 ggcattcatc aaatctgggt gtacaaagac tagacctaac acaatcccaa gaagaggatt
      841 tcctttaaag tattttacag ttgaccatgt tattaacgca ggcagaaatt gaaaggaagt
      901 gtttgcgata agatttatca tatcagcgac atcagcaata ttaggatata cttcaatgag
      961 tgactgctca aaaaagattc ctgggtttgt taataaatta ttcagtccca tcagtaaacc
     1021 agctgtaacg atcgctggta aaatcggcac aaatacatca cccaatattt taactgcacg
     1081 ttgaaaccaa ttcaaattca actcagccgc tactttttta acgtcttctt tgtttgcccc
     1141 actcagccca gattggctta taacctcttc atagatatct gctactttag gaccgataac
     1201 aacttgaaac tgtccattag cattgaaggt acccttcaca tcttccaggt tttccaatcc
     1261 tgccttatca actttagtgt catctcgcaa ggcaaacctc agacgtgtca cacagtgagt
     1321 cagtgctgca atattctctt ttccaccaac aaagttaatg attgatttag cttcttcttt
     1381 attactcatt aattcttacc tctatttctt ctttattagt taatgcttga gatgaatcat
     1441 tgcctaccat gatccaataa gcaccatcat ctataatttc gttgaactct tcatcataat
     1501 acttaaaatc tttgtttgta agttcaaaag aaacagtcac actatcccct gacttaacaa
     1561 aaaccttctt gtaggctacc aagtcttttg ttggtcttat ataacgacca tattgagctc
     1621 caatatatag ttgaacaacc tcataaccat ctctacttcc tttatttgaa acggtagtag
     1681 tcaccttcaa acagttctta cccttctctt taatgctaat tggtgacata gaaaaagtgg
     1741 tataactcaa cccataccca aagggaaatt gtggttcatt ggatacatct aagtatttgg
     1801 tcgtattaaa cttttcactt gaatacggtc taccagtatt gaaataatta taataaatag
     1861 ggatttgtcc taaatgtctc ggaattgtta ttgtaagctt tccggctggg tttattttgc
     1921 cacaaaggac gtccaaaata gcattgccag cctctgctcc tagatgccaa gcctcaacta
     1981 atcccttaca tatagatttt taaattacac actagtttaa actaaaacaa acaaggaaaa
     2041 acttaccatt taatcaaaaa attagctcat atgaatatat tagaatgtaa attagactgg
     2101 tcaaaatctg gtcaaaaaag aggacgaata caggtagtcc tcttttttgt tattttgata
     2161 ttctaaatca ttttttattg gattattccc ttgttcgata tagacccaaa ttgatctatc
     2221 aataattatc aaactgagtc atatagagac aaaaatttca aaaatgacct gttaaatggt
     2281 catcgaatat catttaaaat caatacgacg gagtcagaca cggagtcaaa acaagcccgc
     2341 tgagagtaaa aaatatacag tcccataggt aaagtgccac aggactgtat atttttttat
     2401 ttgagattac ctgaaatgat tccatttaat ttttatctca ttttttccta ctagttagta
     2461 tcaaatactc tcctattttc tcaataaact attttgggtt acatggtatc tgaaacagct
     2521 aaaaccttgt cattatagca aaaaatcact ttatctccgt ttcatttcag atacatttca
     2581 cttaagcgga ttccaaaaaa acaaaagaaa aaggaacccc tagttagcct atgcttttta
     2641 tt
//
LOCUS       DAZKCY010000203         2550 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000203 DAZKCY010000000
VERSION     DAZKCY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.208"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..1431
                     /locus_tag="ACG8XC_002757"
     CDS             28..1431
                     /locus_tag="ACG8XC_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9192398.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            1780..2412
                     /locus_tag="ACG8XC_002758"
     CDS             1780..2412
                     /locus_tag="ACG8XC_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFP9192399.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          958 a          365 c          504 g          723 t
ORIGIN      
        1 taaagagaaa aaagagaggt aaatcaaatg aaaaaatctt taaaacttgc atcagcagtt
       61 atattaatgt cccaactcct tatagcaaca cctttccatg tgttggcaag cgaaaattca
      121 acatacaaaa ttgcaaaaga aagaaccgag acgactgaaa tgagtaattc cgatgtaaca
      181 gcagagagta gtgaactttt gacaacttca tcaagtgaaa gtagtacgac ttcaaatgcc
      241 gagtcttcaa caacttcatc aagtgaaagt agtacgactt caaatgccga gtcttcaaca
      301 acttcgtcaa gtgaaagtag tacgatttca agtaccgaat cttcaacaac ttcaagtagt
      361 acaagtgaaa taacttcttc gaaagtgaca aaaccaaacc atatagaatc ttcaaaaaag
      421 gaagcgccaa aaacggaagg acaaacaaaa actttttcga caccacagca aatagaatca
      481 acagaatctt tccaatcaca accaatacta tctggtaatg aagaaattca ttttgacaaa
      541 aatgaatctg tagaaaattt tattatgaaa attggtgagt ctgcgagaaa aattggtcag
      601 aaatatgatt tatatgcttc tgttatgatt gcacaagcga tcttagaatc tgccagtggt
      661 caaagccagt tagctcaggc accgaattac aatttatttg ggataaaagg aacttataat
      721 ggaaactttg tcattatggt aacaaacgaa gatttgggta atggaacgct ttatacgacc
      781 caatcaaaat ttagagtgta tgaaaactat gaggaaagct ttgaagatta tgccaaatta
      841 ttaactaagg gaattagtgg aaataaagat ttttatgcag gagcattaaa agctaatagt
      901 aaaacatatc gagaagcaac taaattcctt acaggacggt atgcaacaga tacacaatac
      961 tatttgaagc taaatgaatt aattaaaacc tacgatttaa ccaactatga taaagaagtt
     1021 aaaggtccac aaatgagtaa gactggatac caagtgccta ttcataatta ttcaataacc
     1081 agtttgtttg gaaatcgtgg aaatgaattt caccgaggac ttgatatggc agctacacag
     1141 ggtgagccga ttcatgcagt taaagctggt aaggttgtta aagcagagtt tcactactct
     1201 tggggaaatt acgtggtgat acaacacgaa gatggatcaa ccgcgttata tgctcaccag
     1261 caacaatacc tagtaaaaac tggagataat gttttgcaag gacaaataat tggctatgta
     1321 ggaagtactg ggaatagtac tggcccacac cttcatttgg aaatttgcaa agatagtagt
     1381 ctttctcaga gcatgttagt tgatccgaaa accttattat tcggagacta aaaaaggtaa
     1441 tcaatctgac aaagtaacaa gaaaatggtt ggattcttag acctttgatc aggatattga
     1501 gactggaatt gatatctcga ttacgatggg tttgacaaat aaaacaagtc cattatcgaa
     1561 tatcgagtgt attattgccg tctttatgct catattttga gcaggtttca ctagatgaaa
     1621 acaatttatc tatcttgagg atttcgtgta cgtatcgttc agtcttgtat tgtaaattag
     1681 tcataaaatc agactaagaa aaattggaaa tatttttttc tgatttatta aaagttaatt
     1741 gaaattatat ggtacttctt tcttgggagg gtaggtgcag tgaaggaaag atatggtcac
     1801 cttactgttg ttgaggagat tactcataat ggatataaaa aatatcgttg cctatgtgac
     1861 tgtgggaatg aaacgattgt atatcgatca aacttactat caggtagaac caaaagttgt
     1921 ggatgtggaa attataaaaa taggcataaa tataaaaatt tgacaaataa aacttttggt
     1981 aagttagttg caatcaagcc aactaaactg cgaagtgaag gaactgttgt ttgggaatgt
     2041 caatgtctgt gtgggagaaa aaagttagta tcggcaaagc gacttctaaa aggtgatgta
     2101 aaaagctgtg gatgtttgaa atcaaaaaag catcacattg aaaataaaaa attcggatta
     2161 cttactgcaa agtatccttt ggacaataac tgttcatgga aaacaaaatg ggtttgtcat
     2221 tgtgagtgtg gcagtatgtg tgagataagt tatagtaact tattttttgg acatactcaa
     2281 agttgtggat gtttaaaaag gcaagaaaca aggacatgtg ttgaagggac agtaatagag
     2341 tgtctaacgt caaaattacc gaaaaataat acaagtggtg ttaagggagt ttatcgttct
     2401 aaagagaaat agattgcgta tataacttta aaaaaaaaga ggtactattt aggatcgtat
     2461 gattctatta agagtgcagc tcaagctaga aaagatgctg aaaataaatt gtttcaacct
     2521 ttgattgaca aatatcgagg taatcattaa
//
LOCUS       DAZKCY010000204         2542 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000204 DAZKCY010000000
VERSION     DAZKCY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.209"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1168
                     /locus_tag="ACG8XC_002759"
     CDS             14..1168
                     /locus_tag="ACG8XC_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFP9192400.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1141..1584
                     /locus_tag="ACG8XC_002760"
     CDS             1141..1584
                     /locus_tag="ACG8XC_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192401.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
BASE COUNT          892 a          431 c          440 g          779 t
ORIGIN      
        1 ttaacacaaa ggcgtgatta ttttgaaacc caaaaagaaa atttatctag gtctcctctt
       61 ttccttgatg attggaattt ttagtatagg aattacaaat acctatgctt cagaaaattc
      121 taatttcaat atcaactccc aaccaattga agaacaagca attaattttg cgaaaactga
      181 ttttgaacac tccataactt caattctttt aatggaaaat aacaccgaaa ctgatattaa
      241 ctatactctt ggttcgccat ttaaaatgct cgatggagaa aatacggcaa atagttctat
      301 tttctttcca gtattttcag acgctgataa tacgattaaa tatgtttata ccgtcaaaaa
      361 aaatacaaat ggagaatatt caggaagtat ttcacaattt ttagcaaaag aacttcaaaa
      421 tctagtagat agtaatataa cagatacaac cttatttttt caagataata gcatttatta
      481 ttccgataat ggacaaatta aactactttg gaaatcacct ttaagtacag aaaaaaatgg
      541 tgtttcttcc cttacagaaa aaaatgatct tatatccgta aatataaaaa ataaaattaa
      601 aaatttttct aaaaaacaaa attttcctgc ggttaaacgt gtaaaaagtg tctcctccgg
      661 agataacttt attatgatta attggcgtat agcagaaacc caaacaagtt ttccgtggtg
      721 cgctgcttac gtagaagctg ctatcttatg taacaaaaca gatgtacgtg ctacttcagc
      781 tagagccatt atgcattata cctatcctaa tttaaacaac agtcaaattg aatcaaaaag
      841 tcttaatcaa gatcaaataa taaaatatgc aaattactgt ggtgtttatc ctaaaaaaat
      901 agcacgaata ctaaaccttt cagaggtcca aacacaacta agaaaaggaa atgccattta
      961 tatgggaact gctggttatg gaaattatag taacagccgt catgcacttg cattatttgg
     1021 gtgggtcaat caaggtggtt atcaaactta ctatgtgtgg aatccttggt ataactaccc
     1081 aactgtaata agtgcaaatg caggaccagt tacaattcct gtacctggag gtggttttac
     1141 ttgggacaat accattacaa attggtaaat aaactcattg ctttagttat gggtctagtg
     1201 ttagttcttt tcttaactag ttgttcaaaa aaacaagaaa tacaaaagac tgatctgatt
     1261 ccttttctag gaacttggca aactaaagat cattcagaac gtctagtaat cactaattta
     1321 aatacttcaa aaaatataga acaatatacc atcttagtaa aagagaacaa tcataaaatt
     1381 caaatcattg ataaaaattt aaatataaat gaacaagttt tcatttacaa agcaacaaat
     1441 aatttactaa cactgcgctc gaataaaaaa caattagatt acacttttag tttggattct
     1501 ccaaaaaagc tcgtatttat tttgacaacg aatgatggga cagaagggac agctaaacca
     1561 attgaatttt ttaaagctga ttgatgaata gttgtttgat attacaaaac aataatttca
     1621 ccgaaggtga aacaagcgaa actttacaaa aagtggctag catttgctag ccttttttgt
     1681 attgtggagc gtgggttttg agggggattc aagggggagc ttgcgcccct ttgacaagcg
     1741 gttatttgca ccaaatacgt aggtatttgg ctagcaaata tagagcttgc caagcccttt
     1801 agtgtgtttt gtagaaaggg accttatagt cactttcaca ttacaaacga tacgtcctat
     1861 ggacgtatca aaagggtggg acttatgagc aaattaaagc aaaatttggc tagcaatcct
     1921 tctaaaaaat acacctattg atcagagtca aaataaatca gttttcgagt gagcgaatcc
     1981 aaatttgcaa ctctgaagct ttgcaaatga gtgtgccggc ttccgtaaaa gtctaggtaa
     2041 cattagtcga tatatacata gggctctaat ttgcgtttta aggcgtttta aacgttctgg
     2101 tatgtaatta taccaaagcg actttaaaac gccttaaaac gaattttaga ggttctgtag
     2161 aacgttttag ggttgttagg gcttgccctg accgcctgta aggcgcttta aaaacaagat
     2221 aagggatttt atctagactt gtcttaaaac gccgtagatg agcgctcaga gcttctaaaa
     2281 ggactcctta cgaactgttt ttaagcaaag gagcctttag gcgactgcgc agtttaatgt
     2341 gagagcgtag cgatcacacc tttaaagcga ccgtagggag cagattccca gtttaggaaa
     2401 tttcgcgaac ctgtttggag aaaaaaagta ggaaaatttt aggcgggaaa ttttgccccc
     2461 ctcttttata atctctttta taacctcttt ttaaacctct ttaccgtcgg ctgtatcctt
     2521 actctcccaa ggtgtgcaga at
//
LOCUS       DAZKCY010000205         2538 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000205 DAZKCY010000000
VERSION     DAZKCY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.210"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..164)
                     /locus_tag="ACG8XC_002761"
     CDS             complement(<1..164)
                     /locus_tag="ACG8XC_002761"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9192402.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINAL"
     gene            complement(495..728)
                     /locus_tag="ACG8XC_002762"
     CDS             complement(495..728)
                     /locus_tag="ACG8XC_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFP9192403.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(743..1693)
                     /locus_tag="ACG8XC_002763"
     CDS             complement(743..1693)
                     /locus_tag="ACG8XC_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFP9192404.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1690..2532)
                     /locus_tag="ACG8XC_002764"
     CDS             complement(1690..2532)
                     /locus_tag="ACG8XC_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFP9192405.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
BASE COUNT          844 a          475 c          320 g          899 t
ORIGIN      
        1 ataagcgcat taatatgcgg ctgaataatt tcttcaaata acgcttcctt agaagtaaaa
       61 tgtttataaa aagcacccgt agttacgcct gcggtcaagc acagttgcct tatcttagtt
      121 tgctgaaatc cttctttttc aaaatggctt agtccacttt ctattaattt ttggtgagtt
      181 gtaatataat ctctggtttc tatgggtact tcctcctaat aagatgaatt aactatatta
      241 tcttacacag gacttgaagt ttcaatcgtt aacgtctatt actgaactca ttccttagag
      301 ctaaactaat gagaaagagc aaaataactg atacaaaaag caatcctaca agaattaata
      361 gactcatcta aatcattcac cttttctaaa ataatccgag aaagatttaa aaaaattata
      421 tccagctatt agggtaagtc ctatcataat cactccaata gccattttct tatcctccct
      481 aaaaataaag tcttttactt aaaacctttt ttgtaatcgt caataaattt tttcccttta
      541 tcagttaatt gaaatcctat atctaaatag tcctcatatc taagtttagc cagcatctca
      601 cttacagatg ctggactaac acttagaact tttgctaaat cattattttt taatttcttt
      661 tttctttttg ataattcctg aattgctagt aaataaatta gtatagtagt cgttttaatt
      721 tgtaccatac ctaccctcca aattatttcg aaaggccttc agcaatttta tccaggttcc
      781 atttcatcat cgcatagtaa ctatcaccat cttgtccttt ttctgcaatt gaatcagtaa
      841 aaatcgttga atagatagga atattggtat cttttgatac tgttttcatt ggtctatcgt
      901 ccacactgct ttctacgaat aaggagggaa cttttgttgt gcgtaatttt tcaactaagt
      961 gttttatttg atctggtgtt ccttcttctt cagtgttgat ttcccaaatg taagcagaag
     1021 gcacattata cgctttcgag aaatatttaa agcatccttc acttgtaaca atcatttttt
     1081 tatcattcgg aattgaagca aatttagatt tagcttcttt gtctagagaa tccaattttt
     1141 caatatactt atctagattt tctttataga attttttatt atcaggatct ttttccgcta
     1201 actgtttttc aatattttta gcgtaaataa taccgttttc taaatttaac caagcatggg
     1261 gatcttcctt ccctttctca ctctgaccct ctaagtaaat aacatctatg ccatcacttg
     1321 ctgcaaaata gtctttgttt tcctctttgt tcgcattttt tactaatttt gtaaaccaag
     1381 catttcctcc agtttccaag ttaacaccgt tataaaaaat caaatctgct tttgaagttt
     1441 tttgaacatc ttcaggcaaa ggttcatatt cgtggggatc tttcccaata ggtacgatac
     1501 tgtgtaaatc aattttatct tttgctatat tttcagtaat atctgctaaa atcgaattag
     1561 taactactac ctttaatttt tcgttcgaag ccactgtatc tttgtcttta ctatttgttg
     1621 aacatgcccc gaatataaaa agagcaatca aaatccccag acttcccagt actgctttcc
     1681 attttctcat cataactttc tctccttctt tcttaaaaat ttttgttttg gtgagattag
     1741 aaaactaatt acaaataaga atgccgctgt taaaacaata cttgatcctg ctgctatgtt
     1801 aaaactataa ccaatgaaca aacctagcaa tgatgagcac gctccgactg tcgccgaaat
     1861 aaatatcata gtttttaagc tgtttgcata taaatatgct gtagcagctg gtgtaattaa
     1921 cattgcaaca attaatatag ttcccacact ttgcatagct gttactgcta ctaaagttaa
     1981 tagaatcatt aatagataat gataaaaact gacattcatc cccattgctt ttgccattaa
     2041 tggatcaaaa gaagtgatta ataattcttt aaaaaataga acaactgtaa ccaacactaa
     2101 aacagaaacg ccaattgtaa tccacttatc aatatcttgt acagctaaaa tatttccaaa
     2161 taagatatga aataaatccg ttgagctatt tgcaactcct ataaggataa ctcctaatgc
     2221 taaaaaagaa ctgaaagtaa taccaattgc tgtatctcct tttattacac tattgctttt
     2281 tataaaagta ataatgatag aagctaatag accaaataca atcgcaccaa taaaaaaatt
     2341 aatacctaaa atataagaaa ttgctactcc tggcaaaaca gcatgagaaa tagcatcacc
     2401 cataagagac attcctctta aaataataaa acaacctact gttccagaaa caatccctat
     2461 aactacagat gtaattagag cattctgtaa aaagtgaaag tcatacagac cattaataaa
     2521 attttgaagc atattatt
//
LOCUS       DAZKCY010000206         2533 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000206 DAZKCY010000000
VERSION     DAZKCY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.211"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..387)
                     /locus_tag="ACG8XC_002765"
     CDS             complement(49..387)
                     /locus_tag="ACG8XC_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFP9192406.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(403..606)
                     /locus_tag="ACG8XC_002766"
     CDS             complement(403..606)
                     /locus_tag="ACG8XC_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9192407.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(621..1397)
                     /locus_tag="ACG8XC_002767"
     CDS             complement(621..1397)
                     /locus_tag="ACG8XC_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP9192408.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1696..2016
                     /locus_tag="ACG8XC_002768"
     CDS             1696..2016
                     /locus_tag="ACG8XC_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9192409.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            2034..2465
                     /locus_tag="ACG8XC_002769"
     CDS             2034..2465
                     /locus_tag="ACG8XC_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9192410.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT          769 a          429 c          402 g          933 t
ORIGIN      
        1 agttctaaag cgtagatgat tagcggtgcg ctgaacacta gcgctctatt aaattttccc
       61 atccgcttgc ctccttaaga tttccgaaaa atttgtttct aaaaattcaa gtgttttact
      121 tcttaaaaat agatatgtgt ctcttccttc aactggataa tagacaaatc catttttgtt
      181 tttctcgata tcgataatat ttctatatct tgggttcttt aaaactctag aagtaaacca
      241 atcatatttt ctgttaatcc gttctagcac ttctggcaac gtcatccatc taccagtatc
      301 atcagctttt tttaactcct cataatccac ttgggagata attacatagc cttctggaat
      361 tgggattttt gcttctagat attgcattag ctgttcctcc ctttacaatt cgtacatagt
      421 gataatcgaa tctataattc tgtttgcttc tgcagaagtc tttttaccgt ttaaaattaa
      481 agataagtag cttttgctaa ttccaaatct ttcagcaagc atggtgtaag ttaagaactt
      541 tgaactttcg acatattctt tgattttttc tctatctcgt tgagtgattt ctgcaatgtc
      601 agtcatacta aaactccttt ctaaccaatt tcctctaaat ccatttgagg gtaatatcct
      661 tcttttttta gtaattcgta gataaataga cgccctttct gtgtccattt ggtattcatt
      721 acaattttag tgccaccatc ggctttcggg atctcagttg tatgagattt tgtgtatcct
      781 tggttcatgt gttttttgca caataaccat tggttaccga cttttttctg aatacctagt
      841 ttatgaagta atttattcat ctgttgtgga gacatcccat aatctgctgc aatctgacta
      901 attgttactg aatctgtaga agataatatg ctatctaaat aggagatttt aggttcgtac
      961 tctgcaatct tttgttctgc gattaatctt ccagttcttt cttcttttag ttgagttgct
     1021 aattgaatga ttgtatctgg attaagcaaa gcttcttcta ctttttctgg agttagataa
     1081 cctccatgtt ttctaattgc tggcaacact tcacttgtta cccaacgttt aaattttttg
     1141 gcagagggaa gttttgattt taagattaaa ctgtacaaac cagattcgtt gatgattgtc
     1201 atctctcttg attgacctga ggtcgtgatt cgcgacccca tcttatcttc caaatctaca
     1261 tgccgcgaca aagcatcttt agtgtttgaa taacccaaaa cactcgcaac gtctttgcct
     1321 acaaaatatg gttcatcatt tactaaaatt gtccgaactt cgttttgttc gaaattaaaa
     1381 atttgcggtg tgttcatatt tctcattcct ttcttttata ttcgtaaaca aatttaacaa
     1441 cttttttcta aattcaattg acagtatctg agtaatactc tatactaaga gcataattaa
     1501 ataagacata aaaacattga ttttaaagct ttcttggcgg tctgcattta catatcaata
     1561 gtgttttttg ttgtcttttt agttgttaaa tctgtttaca agatatagta tagctcatta
     1621 ctcagaatta gtcaaattat ttctgcgtat ttttctaaac tttttttgta aacatttaga
     1681 aaggttgttt tatcgatgaa tacctatgaa ataataaaag acttggcgaa gaggaaaaag
     1741 atatctatac ggcagttaga aattaatttt ggatattcta acggttatct agcaaagtgg
     1801 aaaaataata ctcctaattc agatgaatta agtcggattg ctgactattt cgatgtctct
     1861 gtagactacc ttctaggtag agaagaaaga gaaaccccta aacatgtgga tttatcagaa
     1921 gacgatactg ttttttcttt tgatggaaaa gaaatatcta aggagacaat gcgtaaagcg
     1981 attgcaattg ctaaagcttt agaggaaaat gaatagttgg agtgatggtt tgtatgtatt
     2041 tgaaattgaa agaaatgttg agcgaataca atttgaaagt agtttatatg gagatgaaag
     2101 agcctggttt ctactatcca aaacccagaa tcattttttt gaatgaaaat ctatacggtg
     2161 aaactgcaga agcctttcat ttatctcatg aacttgcaca tttcagcgcc tctcattttg
     2221 aattttcagt tttgtacgat acttctacaa cttttcactc aaaatttgaa acagaagcag
     2281 ataaaatagc tattctaatt ttattaaata tctatattga gaacgaactg actgatgaat
     2341 ctcagtttaa tctcgaaaaa tttatggagt attattctat tcagaataag ctcagataca
     2401 cttgttatgc tgtctgccaa tgctatttta agaaaaaata ttcttacgca aggcaatatg
     2461 tatgaataca tcaagaatta ttaaaaaaac cgatttcagc aaaacattaa aaaagcccgt
     2521 gctgcaacac ggg
//
LOCUS       DAZKCY010000207         2527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000207 DAZKCY010000000
VERSION     DAZKCY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.212"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..542
                     /locus_tag="ACG8XC_002770"
                     /pseudo
     CDS             288..542
                     /locus_tag="ACG8XC_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            612..1124
                     /locus_tag="ACG8XC_002771"
     CDS             612..1124
                     /locus_tag="ACG8XC_002771"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9192411.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1121..2158
                     /locus_tag="ACG8XC_002772"
     CDS             1121..2158
                     /locus_tag="ACG8XC_002772"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9192412.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            2318..2494
                     /locus_tag="ACG8XC_002773"
                     /pseudo
     CDS             2318..2494
                     /locus_tag="ACG8XC_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          911 a          462 c          507 g          647 t
ORIGIN      
        1 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
       61 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      121 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      181 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      241 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
      301 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
      361 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
      421 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
      481 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
      541 accccctatg ttaggctagt tgtcgtatag gataataagt tataataaag actatacgga
      601 ggatgattac catgacaaat aaaaacaatg aacgttactc taccgaactg aaagcgacag
      661 ccatcaagaa aatgatgcca cccgaaaata gggggattcg ccaattaagt caaactttgg
      721 gcattccaga agcgactctt tattcttggc gtaaaaaagc tagaattcaa gggaatacta
      781 cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga agataaattt ttaattgtga
      841 tggaatcatt ctcaatgaac caagtagaac ttgcggaata ttgccgaaag aagggcttat
      901 ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc tgcgaatgaa caggaagaaa
      961 atcgaacgag agaacttgca actgaactaa aagaagagaa aaaacaagcg cgtcaattag
     1021 agaaagaatt acgtcataag gaaaaagcat tagcggaagc cgctgctctc ttattactaa
     1081 gaaaaaaagc acacgcgatt tggggggacc aagaggaaga atgatttcat cttcagatcg
     1141 caaacaagct gttgaactta ttcaagaagc caatcgcaat ggggcccgtc tcacctatgc
     1201 ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga tggacgcgtg aaggtacgat
     1261 agcgcatgat caacgccctt tggcgaaacg tcctgtaccc aaaaataaac tgacggatca
     1321 agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa tttatgaatt tgccacctag
     1381 ccaaattgta ccaaaacttg cggattgctc gatttatatt gcatcagagt ctagcttcta
     1441 tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc cgaagtcaag tttcacaaaa
     1501 acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa gtttggactt gggatattac
     1561 ttggctaaaa ggtccaataa aaggactctt ttaccggtta tatctcatca ttgatttatt
     1621 tagccgtaaa atggtaggct gggagatttg ggaatcagaa gaagctcgtc atgccgaaaa
     1681 acttattaaa cagacggtgc ttaaggaacg tatccaagga cagccgttgg ttttacactc
     1741 cgataatggc agccctatga aagccgcgac ttttcaagtc ttactcgaaa aattaggaat
     1801 tcaaagttct tattcaagac cacgagtgag taatgacaat ccttattcgg aagcggcttt
     1861 ccgaacgtta aaatatcgac cagaatatcc caataacggc tttaaaacaa tagatgatgc
     1921 tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat tctcatttgc atagtgggat
     1981 tcaatttgtg tctcctgcag cgtgtcatga aggacgacat ctcgctattt tagagaaacg
     2041 aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa cgctggtcaa aaggtgttcg
     2101 aaattggact ccacatcaat cggtggcgtt gaatccttta aaagaaacag aaagttaaca
     2161 ggagcataat ctttcaagat tatcaaataa ccctcgtcac ccaatagggg aaaaagttat
     2221 tcttccccta ttggataacg aggccaaccg accataggga ggttctgctt tttgataggt
     2281 ggaaaataaa aatacgacaa ctatattgac aaacaccgac catcgtccag taaagagacg
     2341 caataaattc tatcgaagtt tacgcactgc ctctacaacg attaaaggca tggaagccat
     2401 ccgaggatta tataagaaaa cccgaaaaga aggcactctc ttcgggtttt cggtctgtac
     2461 tgaaatcaag gtattattgg gaatcccagc ttaaatcata gataccgtaa gggattttat
     2521 tctttat
//
LOCUS       DAZKCY010000208         2478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000208 DAZKCY010000000
VERSION     DAZKCY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.213"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1139
                     /locus_tag="ACG8XC_002774"
     CDS             <1..1139
                     /locus_tag="ACG8XC_002774"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9192413.1"
                     /translation="ELFTDVSAAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRS
                     IITSMVMTYMFSFEGIKGYGYVVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTA
                     IFMIYLLTIGLFVSYVAHQYQVFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQ
                     QLKRKELVYLKKYTDSLEKDQEKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELE
                     IYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIF
                     DCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKG
                     ITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
     gene            1154..1906
                     /locus_tag="ACG8XC_002775"
     CDS             1154..1906
                     /locus_tag="ACG8XC_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9192414.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
BASE COUNT          909 a          306 c          406 g          857 t
ORIGIN      
        1 ttgaattatt tactgatgtt tccgcagcta tacctataat cggaagttat tttatattaa
       61 aggaaaagaa gaaaactaat atagtgcttt taaataaatt actattatgt atgcttattg
      121 atgatttcag atctattatt acttccatgg ttatgacgta tatgttttca tttgaaggga
      181 taaaagggta tggttatgta gtattccaga taatgcttga ttctttgttt ttgctgatgt
      241 ttatttggtt atatagaaaa ttagctataa ataggattgt tgaacagtcc agttctaaac
      301 ttactgctat attcatgatt tatttactta caattggttt atttgtttct tatgttgctc
      361 atcaatatca agtatttgat cattttgtgt ttggtgtctt aatattttta atcattcaaa
      421 tgtttgttgt tctattttta tttattagaa taactattag gcaacgagaa caatatgaac
      481 agcaattgaa aagaaaagaa ttggtttatc taaaaaaata tactgattca ctggaaaaag
      541 atcaagaaaa atttgccaga tttagacatg actataaaaa tttattgctg agtttaaaag
      601 aaatcgctaa tcaaaaccat gattcgatat tggaaaaaga aataaaagaa ttagagatct
      661 attctaattc ttatctaacc aataagtttg aatatcgtta tttaaaaaat ataaaaaatg
      721 aatatttgaa aagtttgttg atagcgaagc tataccaagc aaataacaat aagatttgtt
      781 gtaaattcga atgtccaagt actattattg aagttcctgt tccaatattt gactgtatta
      841 gggtgcttgg aattattctc gataatgcaa ttgaagcatc tatagagagt caagatagag
      901 aactgtctct agctatttac cgagatgata aacagataga aatttcgata tcgaatagtt
      961 gtagagagat actactctct atagatactc taatgaaaaa aggaataaca acaaaaaagg
     1021 gacatcaagg gttaggttta tctagtatag aggaaatcaa taaaaagaat cccaatatgt
     1081 ttgtgaacta tcaaaacgat ccgtatcaat ttactactca agttattttg ttgttttgag
     1141 ggaaggagga tttatgagtt atccaataat attatgtgaa gatcaaatta tacaattaaa
     1201 tcagttagaa cgaattattg ataatttcat tctttttcac gataaagtat ttaagatagt
     1261 attgaaaaca caaagtccgt tagaagttaa aaaatacctc aaacaattcc gaccaaagca
     1321 aggaatatat ttcttagata ttgatttaaa tcatgaagtt aacggtatag aattagcaga
     1381 agtaatcaga aaatatgatg ttcaagcaaa aatcattttt acaactactc atgatgagat
     1441 gttacccgta acaataaaaa gaagagttga aacgttagga tttgtaacaa aagatcaaac
     1501 actagatgag tatcgaaacg agattgttga gttattgtta ttagcgcaag aaaggataga
     1561 tgcaacaaaa caggttacga atcaagcatt tgttttttcc ataggttctc aaacatttac
     1621 ttttgatata aatgacattt attttgtaga agcttcaaaa ttaccacata gattatcatt
     1681 gtgtactaaa gatgggcagt acgaatttta tggaagaatt agtgagcttg aaaagaaata
     1741 tcctatgtta acaagaatta gtcgagcgtg tttagtaaat atctttaacg ttaaagaaat
     1801 agattttaaa aaacgttcac tatattttga ttcagagttg gccagaaact ttactttagg
     1861 gaaagcacaa aaaattaagg agaagttgaa agaaagcact gtttagtggt tttttcttta
     1921 gggagattgc atttttctaa taaaatagat tccttcacta gtgcaaattt ttctgaatcc
     1981 tattaaagtt gtaccaattt ttatagctgt tattagttac gtgcataaca gcaatattag
     2041 ttactggagg aatatttcta tggtctggaa ccaaaaaagg gccaaatgaa tagttctttt
     2101 cttgttttta ttaagtacca taaaggggaa ttaaaattaa atgtatggtg gaagatgcga
     2161 aagatttggc aaattctact aatttttaga aagtgttttt cattttttag ttaaaaaatt
     2221 tgtcctgtca agtgcaatcc tatgattgta cttgatagga tttgagataa ttactttcct
     2281 gtttttataa atttaacttg caggaacagg ctttaatgca catccgacaa gtacattacc
     2341 aatacctcct gtaacggccg aaataggtcc tcctgcagca ccaactacag cgctgcaagt
     2401 ccttttgtaa aaatatctct accattacat ttccaaagaa tttttatctg actttaataa
     2461 aagataaaaa tttaatat
//
LOCUS       DAZKCY010000209         2448 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000209 DAZKCY010000000
VERSION     DAZKCY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.214"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1296)
                     /locus_tag="ACG8XC_002776"
     CDS             complement(<1..1296)
                     /locus_tag="ACG8XC_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9192415.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEF"
     gene            complement(1307..1975)
                     /locus_tag="ACG8XC_002777"
     CDS             complement(1307..1975)
                     /locus_tag="ACG8XC_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFP9192416.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2106..>2448)
                     /locus_tag="ACG8XC_002778"
     CDS             complement(2106..>2448)
                     /locus_tag="ACG8XC_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9192417.1"
                     /translation="FNELDPFINYIFENKRIFDLVLFQSAGTKYQDIVEKITSFVTKK
                     TVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQ
                     FSVPGWIELLS"
BASE COUNT          852 a          417 c          313 g          866 t
ORIGIN      
        1 aaattccgga tcatgagtgg ctataactac tgcacccttt tcttttaagt ctttaataag
       61 gtcagcaatc ctttccatat tgatataatc caacccactt gtaggttcat caaatagaaa
      121 taaattagtt tttgacaatg aagctataga aaccaataat ctttgttttt ctccaccgga
      181 taaatcaaac ggtaattgat ctttcatttt ataaagttcc atttttttta aaattttttc
      241 ttgctgatct ttactaacta tttcattccc cagggctaac tcgctcctca ctgattctgt
      301 gaaaaattgg taatccattt cttgcattac taaaaacggc gatgacaaat tttttttatt
      361 ttcgattatc tttcctttgg tagggcgaat aatcttcatg agtattctta gtaaggttgt
      421 tttccccaca ccgtttttcc ctaccaaacc aataacatct ccaactttga catcaaaatt
      481 tatgtctttt agaatatttt cataagtaat ttcttcaact tttaatacat ctgcacttac
      541 cttttcgata tagggaactt caatcgaact attaaaaatg tcaaatttcc ttaatgcgtt
      601 attgttttca gtttttaaca actgaatagt tgtagttttt acagttctat ttttaacaaa
      661 tagaacttga tcaattaatt ctttcaaata gtaaaaacga tgttcagcta taacaatagt
      721 aaatccttta ttttttaaat cacaaagtaa gtcagcaagt ttcttagtcg ctttgaagtc
      781 caaatttgaa gacggttcat ccaatataat taatttagag tttagcgata aactacatcc
      841 aatagaaatt ttctgccgtt caccacttga tagcgaaaaa atgtttttat ctaatatatt
      901 tgaaagatct aataattcag ctaattcatt gattctttta tccatatgct cttttgtaaa
      961 tccaaagttt tccatcgaaa atactaactc actcgttgta tttgttgtaa agaattgtcc
     1021 tctaggattt tgacttacgt agccaatatt ttttgatata ctttcttgtg ttaaggattg
     1081 tagcgagtta ttttcaaata aaatttcccc aaccaatttt ccgtcataaa gtcttggaat
     1141 aagtccattc attaacttta atagtgtgga tttcccactc ccagaagaac cagtaatcaa
     1201 tgtaagactg ccttttttta ttgaaaaatt aaggtcaaca agcgcttgtt ccttaacatc
     1261 cttatatgaa aaagaaacat tgttaaattc aaacaattct ttcacctcaa atcaaaatac
     1321 ttaagaaaaa aatagtaacg gaaaacagga tgatatagtc taataagctc atgtttgttt
     1381 tatagtaaga acttcttttc acaggagatt ctatcccctt tgttattgca ttagcggtca
     1441 tttcttcagc aatcatagtt gcacgtataa gaacgggaac taacagaaat tccaaatatt
     1501 gcagaggctg aaaaaaagac aagcgaatgt ttctcatttt taaagattct ttaatcattc
     1561 ttatttcaga acgataagtc ggaaagaatc tgatcactac tgtaatactt aacattattt
     1621 ttttcggtaa atgtattttt tctaaagagg ataataattc attgctagta tagacactaa
     1681 ccaacaacca tgagatcatt acgaccggca acattcgaaa tacaatatat aaggatgtcc
     1741 caaaaaatat agtaaaaaac tctatcttga tagcatcaat aattttcatt actaaaaaca
     1801 gaactgaata aatcaagatg aaataaatac atgtctttat tttacgaaac atcaatacta
     1861 aaactgtagc aataatgaaa caccccactt gaaatatatt tccctttcct aaaaaaataa
     1921 tgaccggaaa aaaaatattt aaagtaatca aggttaacgg attgattttt gacactcttt
     1981 cacctccaaa aaataagatc gcactgttat cttaagctga caaaaatgca ccgttattca
     2041 gataacagtg cgatcttatc aaaaagcata actgagaaca attctcaatg caagcttttt
     2101 tttaattaac tcagtaattc aatccaccca ggaacagaaa attgatacaa tcttctggtt
     2161 aatagcatag tcttttcttt tgaatagcta tgcttcaata tatcatagaa tgtagcgtag
     2221 aatgagtaaa tgtagaagtg gatttcttct tcattaaaga ctaaattttg attgatcatt
     2281 ccgatagtct ttaacttatc tagacaacag actgtctttt ttgtcacaaa tgatgtaatt
     2341 ttttcaacaa tatcttgata tttggttcca gctgattgga ataaaaccaa atcgaatata
     2401 cgtttattct caaaaatata attaataaat ggatctaatt cattgaag
//
LOCUS       DAZKCY010000210         2434 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000210 DAZKCY010000000
VERSION     DAZKCY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.215"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..2287
                     /locus_tag="ACG8XC_002779"
     CDS             134..2287
                     /locus_tag="ACG8XC_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFP9192418.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          831 a          427 c          539 g          637 t
ORIGIN      
        1 ttgctgtgaa acaaaaagtt gattttatct tgaaattagg taaagaattt cttgctccgc
       61 ccattttttc ttttttatta agaagttttt gggtacaatg aagcatgaat gtaaataggc
      121 ggaaggatag gaaatgatga aagattttat gaaaaaaata ttaaatactt cttggttaga
      181 aaaaataaga aaaggtgcac aagaacctaa gaacaaaacg tttcgccggt cccatgttcc
      241 ttttcgctta aattttcttt ttttcattat atttacgtta tttgttgcgt tgattgctag
      301 attgggttat ttgcagatcg tgaacggcga agagatggag gcgcgagtga agtctacttc
      361 tacgattacg gtccaagaaa gtactcctcg cggaatgatc tacgactcca caggaaaagc
      421 tttagttacg aataaagcga atcaggccat cacatatacg agaggaacac aagttacggc
      481 taaagatttg cttgaaatcg caacgaagct gaactcgctg atcgatgttc cggtagatga
      541 aaatctgaca gatagagata aaaaagattt ttggctggct gatcccaaac atttgaaaga
      601 agccacaaaa agactttcgg caaaagaaaa gcaattaggg aacagcgaac aatatagtgc
      661 gatggtcaat aaagtcacag ctgatgagat taatttaaac gaggagcaat taaaaattgc
      721 taccatcttt aaacggatga atagtgcata tgaattaaac actgtctata tcaaaaactc
      781 tggtgttact gatcaagagt tagctgttgt ggcagaacgc gcttctgaac ttccaggagt
      841 ttcgactggc acagactgga cacgagaata taatgcagca agttcactga aaagtattct
      901 tggcagtgtg acgactgaaa agcaaggact tccagcagat gaagcagaga aatacctagc
      961 aaaaggttat tctcggaatg accgtgtagg acaaagttat ttggaaaaac aatatgaaga
     1021 tgtcctgcaa ggaaccaaaa cccaatatga agtttccctt gataatgaag gaaatgtttc
     1081 gaatcaaaaa gaaatcttct ctggggaaaa aggttctaat ttaatgttat ctatgaatgc
     1141 agaattccaa tcaaaagtag aagaaatttt gaaaaggaat tatcaaacat tgatcaacaa
     1201 cggcaaagca cagtattcgc ctggtgctta tgcagtggct atgaatcctc agacaggaga
     1261 agtgctagca atgactggtt tcagtcatga acaaggttcc aaagaaatca ccgagaatgc
     1321 tttaggcacg attactagcg cctttgatcc agggtctgta gtcaaggctg gtactttgac
     1381 agcaggctgg gcaagtaacg cgatcagcgg aaatcaagtt ttgattgatg agccgattcg
     1441 tctgcaaggt gcttcagaaa aaagctctgt ctttaatcga agcggacaag ttgctcttga
     1501 tgcagtcaaa gcatcggaat tgtcttctaa tacgtatatg atcaaagtgg cgttaaagat
     1561 gttaggacta gactatacac ctggaatggg actacctagt ttagatgaag aagctaaagc
     1621 ttatcagcaa ttgagagact ctttcaaaga gtttggtctt ggtacaacga ctgggatcga
     1681 tttgccgaat gaatctcctg ggatctcacg ttctgttgat tatatgaaga agttcaatgc
     1741 ggataatggc aaagaatggt atgctccagg taactttacc gatttggcat ttggacagtt
     1801 tgacacatat acaccaatcc aattagctca atatgcttca acagtcgcta atggcgggaa
     1861 acgagtacag ccgcacctag tcaaagcgat ctatggcaat gacgaaaatg gaaacttggg
     1921 tgaagtgaag aaagaaattg gcacaacagt agaaaatacg gtcaatattt ctgcagaaaa
     1981 tatggcgatc ctccgtgaag gattccacca agttgtccat ggtaccgatc cttacacaac
     2041 agccaaacca ttagccagtg cgaaaatgga cctttccgca aaaacaggga ctgcagaaac
     2101 ggttgcggaa ggtcatccag atattgcgac agtcaatagt aatatcgtgg catacggacc
     2161 aacagacaat ccggaaattg ccatcagtgt tgtcttgccg aacttattag acgaacaaga
     2221 ccacataaac ttgacgattg ctaaagaaat aatggacgcg tattatgata tgttcatggc
     2281 aaaataaaaa agaggtcgtg aaaaagccgc ttagtttcga gtatgaagaa aaattttgtg
     2341 aaaatagctt ctcacatttt tgcttaaatt tgacttcata ccgagaaact ggcttttgaa
     2401 caccgtttac tcggcaaaaa aggttgtgac aaaa
//
LOCUS       DAZKCY010000211         2386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000211 DAZKCY010000000
VERSION     DAZKCY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.218"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..2059
                     /locus_tag="ACG8XC_002780"
     CDS             197..2059
                     /locus_tag="ACG8XC_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9192419.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2386
                     /locus_tag="ACG8XC_002781"
     CDS             2059..>2386
                     /locus_tag="ACG8XC_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9192420.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKN"
BASE COUNT          858 a          353 c          465 g          710 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaata
//
LOCUS       DAZKCY010000212         2380 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000212 DAZKCY010000000
VERSION     DAZKCY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.219"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..592
                     /locus_tag="ACG8XC_002782"
     CDS             <1..592
                     /locus_tag="ACG8XC_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9192421.1"
                     /translation="VILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLEYASRDSGY
                     DGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSFGNLHYQYF
                     HHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVFFFVYNTTL
                     RGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            589..1308
                     /locus_tag="ACG8XC_002783"
     CDS             589..1308
                     /locus_tag="ACG8XC_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFP9192422.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1323..>2380
                     /locus_tag="ACG8XC_002784"
     CDS             1323..>2380
                     /locus_tag="ACG8XC_002784"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9192423.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITK"
BASE COUNT          854 a          260 c          472 g          794 t
ORIGIN      
        1 cgtaatactt gtgtcgatag tattatcctt ttttataaca atatttatta cattgaacga
       61 taatggacaa agctatgctg ataatttcac aattcaaggt cttttagaat atgcgtcaag
      121 agattctgga tatgatggtg ttggtgattt aaatcgaata actggtatct ctagagtagt
      181 agatgaaata tttaataata attttagatt agtcctaatg ggagtaggca ttggcaatgc
      241 agagtttacc aatttttttg tcagtgagtt ttataaatcg tttggtaatc ttcattatca
      301 gtattttcat catatttggt tatttataga agttggaata ttaggagttt cgatattcct
      361 attttttata ataaagacat ttttcaatgc aaaaagatct tttatgaagg ataaagatgc
      421 aagtcttgtt aaagttattg caattatgtc ggtattcttt tttgtgtata acacaacact
      481 taggggtgaa acaacggggc cactgattta tatgatttta gccatgcctg aagttgtgaa
      541 aaggaaagag cattattata gtgaaaaaaa tacaaaagga gagatattat gattcctaag
      601 aagatacatt actgttggtt cgggggaaat ccacttccga aaaaagatag aaagtgcata
      661 gaaagctgga aaaagtattg ccctgattat gagattattg aatggaatga atcaaattat
      721 gatttaaata agaaccgtta tatggccgat gcatataaag aacgtaaatg gggttttgtt
      781 ccagattatg caagatttga tattatttat aatcatggtg gattttattt agatacagat
      841 gttgagctaa ttaagtcgtt agatatactt cgtgaaaatc atgcctatat gggatttgaa
      901 ggggaaatct gggttaatgg cggtattggt tttggtgcag aagaaggaca ttctgttatt
      961 aaaggtttaa gggatattta cgattcatta agtttttatc aaaatgatgg tacattgaac
     1021 ttgaaaccga gtccgtatta tattacagat tatcttgaat ctattggatt ggttcgcgat
     1081 aacagcgaac aaataataaa tggcctgcat atctatccta cggattattt tgctgccaag
     1141 gattatgata ctggaaaaat ttataaaact agtaatacaa taagcattca tcaatataat
     1201 gcatcttgga aaacacctaa acaaaaattt gtttatagat taaaatcaac aattggacaa
     1261 aaaaaattta acaggttact tgcactgaaa aaatttttac acttataaag gagaaaagta
     1321 ctttgaagaa aatttgtttc attagtggtg taatttctcg ttctggggga actgaaagag
     1381 tagggagtat tattgccaat agcttgtctg atttaggtta tgatgtcact attttaagct
     1441 tttggaatag tggagagcct tattttgggt tagataagac gattaaggtt gattatatgt
     1501 taaatcctaa aacggaggga aaattatata gaacttattt atatccggtt tggaaattgc
     1561 acaagtatat atgcaaaaat gaatttgata tagtaattga tattgatact ttgttgtctt
     1621 tgtatactac aagggcaatt aaaggaacaa agtgcaaatt gatttcttgg gaacatttta
     1681 attactggac aatggaaatg ctgaaagaaa agaagagatt caaggctaaa aaattgataa
     1741 aaaaatatgc aaagaggctt gtggttctca cagaagaaga tcgtcaaaaa catattgatg
     1801 aatatagttt atcagaagat tttgttataa ctatgccaaa tccgtgtata tctaatatcg
     1861 agtacgaata tcattatgat aaaaagagat ttcttgcagt agggcgatta gtggaagaga
     1921 aaaattttgc tgacatgata agtgcttgga aaattgtaga aaaaaaagta gatgattggg
     1981 atcttatcat tgtaggaaaa ggtgaattag aaaaagatct tcgccagcag ataaaagatt
     2041 taaatctgca aagagtaact atagcagggc attcagacca tgtggaggaa tattataaat
     2101 ctgcatcttg ttatgtgctt agttctgttt atgaaggatt tcctatggtt atattagaag
     2161 cacagtctta tggattacca gttatttctt atgattgtaa aactggtccg agagatttgg
     2221 tacatcataa ttttaatggg atgttggttg aagataagaa tatagaccaa ttagctaaga
     2281 gcatgataat gtttactaag aatactgatt tagcaatgag aatgtcactg aacgcatata
     2341 ataatgtaca aaaatttaat ttgaaagaaa ttacaaaaca
//
LOCUS       DAZKCY010000213         2375 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000213 DAZKCY010000000
VERSION     DAZKCY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.220"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..75
                     /locus_tag="ACG8XC_002785"
     CDS             <1..75
                     /locus_tag="ACG8XC_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFP9192424.1"
                     /translation="PETAIRMEIDQLNRQQRLFGDDNE"
     gene            253..594
                     /locus_tag="ACG8XC_002786"
     CDS             253..594
                     /locus_tag="ACG8XC_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192425.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            587..1258
                     /locus_tag="ACG8XC_002787"
     CDS             587..1258
                     /locus_tag="ACG8XC_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFP9192426.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            1264..1950
                     /locus_tag="ACG8XC_002788"
     CDS             1264..1950
                     /locus_tag="ACG8XC_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFP9192427.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            1953..>2375
                     /locus_tag="ACG8XC_002789"
     CDS             1953..>2375
                     /locus_tag="ACG8XC_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9192428.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKP"
BASE COUNT          889 a          394 c          482 g          610 t
ORIGIN      
        1 ccagaaactg cgatccgtat ggaaattgat caactgaaca gacaacagag actttttggt
       61 gatgacaatg aatagagctg aagcgctaag aatagggacg gcaattgcta attgctggtg
      121 gaaatactat aaaccaatca tcctaagcca acaacatatt gacaagcaaa aagcatggca
      181 acaaataaaa aagtgactcc gccggcaagc aaagagtcac aaagaaaaca catcataagg
      241 agattttagc atatggaaaa agaactttcc actctagatc aatatttgat tgatcctgat
      301 tggggcaagc cgaaaattga ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg
      361 aatgaagaac tagcttggga tcaagatgat ttaggcaacc atgtaactat ttgggatcat
      421 gtttatctta tccatctatc gaagcattcg aataaacctg aatatattta cgtcatcgaa
      481 gatggcttga ttgatgcgct agaagagtac gacagagata acttgattga tatctcttat
      541 tacggaccag gtaagaaata cattgctgaa atggaggcag aatttgatga gtgaaatcaa
      601 agggacaacg aactttgaaa aactttttag tcgtaagtta aataaaattc tcaagaaaaa
      661 aggaaatttt gattatttat cttgggctca cgcgtgggag attatgaaaa agaatgatcc
      721 acaggcaacg gtaactatta atgagtacaa acactacagg gttgtttctg gaactcatca
      781 agactttctt gttgaggaat ataaaccttt tcttatggat gaaactggga cttatgtatc
      841 tgtctcagta acggttaaag gacacacgga aaccgaatta tttcctgttt tagattatcg
      901 aaaccaacca gttgttaaac caaatgcaat gcaaatcaat aactcattga agcgatgctt
      961 tgtgaaagca ttggctctac acggactggg attatatgta tttcaagggg aagatattcc
     1021 aacaccacct agaatcgata caaagaaatt aaacatgcta gagacgattc tagaagcttt
     1081 caatgagcag atgggtaaag atatgaccaa aaccttaatc gaatatgtta atgagcagac
     1141 agataaatta gggctcttag ctgataacgt tgaaactatt gaacagttaa gctatgagca
     1201 atgtgccttg atggagcgag caatagcagc taagagaaaa gaattagata agaagtgata
     1261 tgagtgttta aaccattaat cgattcatat tcagcggttc tgaaaaagtt caaaggaaaa
     1321 gacataagcg caaccatcaa tgaggaagtg aacattgatc gactaaagac gatgtatgac
     1381 ggctacgatg gtgatcgagt cattgaaatt cgttttattg atcctagacg tttcaccgta
     1441 cagcaacgaa acttcatcta tgcgctgata ggcgatattt ttatcgatac aggcatgcca
     1501 acggacttct ggaaggaatt cttctacttt cgttttgaag gtgtcacagg gcgcaaaata
     1561 agcctgaaag acgaatcgaa tacaactgtg agtgatgcca atgtcttagc aaatatcatc
     1621 ttagatttca tctttgaaca tcatattcct ttcaaagaag gctatgagat tttacctgcg
     1681 aatcaagaat attacttcta caaatgcatc acaaaaagag tctgctgcat ctgtggcaaa
     1741 acaggagctg acatcgatca ctttgacaaa gcgctaggaa gacgaaagcg caaagaagtt
     1801 gatcatgcag agtacacatt tgcagcactc tgcagaatcc atcacacaga gaagcacaaa
     1861 ataggtgtga ttaatttcaa aaataaatat caaatcaaag ggatcaaatt aaaccagaaa
     1921 acaatcaaaa agttaaatat tggagggtaa aaatgacaga acatcgaagt tattatgcga
     1981 ttataccagc caacgtaagg tacgacaaaa gacttaaacc aaatactaag ttgttatacg
     2041 gagagataac ggccttgtgt aatgaaagag gcttttgttg ggcaggcaat gagtactttg
     2101 cagatttata tggtgtgaat aaagagacca tatcgcgatg ggtaagtgat ttgattaagt
     2161 ttggatactt gaatcgggaa atcatttaca aagagggtac caatcaaata atcaataggt
     2221 acctacgaat taatcaatac cctattgacg aaaaacgcaa tacccctatt gacgaaaaag
     2281 tcaaagataa taatacatct attaataata catttaataa tacaaaagaa tatataagag
     2341 agttaccgcc ttcgaaaaaa tcgaaggcta agccc
//
LOCUS       DAZKCY010000214         2369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000214 DAZKCY010000000
VERSION     DAZKCY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.221"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            204..1817
                     /locus_tag="ACG8XC_002790"
     CDS             204..1817
                     /locus_tag="ACG8XC_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9192429.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          747 a          420 c          452 g          750 t
ORIGIN      
        1 tcttcagctt ttaaaaaata tacagggtta tccccttcaa agtattatct atccttaaaa
       61 tagaaaaaaa tttttaggtt ttatccacaa attatttctc tatattttct gtctattcaa
      121 aataaacaat aattttccgg cacaattatt tgctgttttt taccataaca tagtacgatc
      181 aacgtagaaa aggagatgtt tttatgaaag ttgttgtcat tggcggtgta gctggcggtc
      241 cttcatttgc cactcgtttc cgtcgattga atgaagcgca cgaaatcatt atctatgaac
      301 gcggagaaaa tatttcttat gcaagttgtg ctttgcctta ttatttagga ggtgtgatca
      361 ctgaccgtga ctcgctgatc gaacgtacac cagaaatatt gaaaacaaaa aacaacatcg
      421 atgtctttac taaacacgaa gtaacagcaa tcgatccttc tactaagcga ttaacagtta
      481 aagacctatc cacaaatgaa gaaataaaaa cagattacga taagttgatc atctcttctg
      541 gtgctagacc agattatccg gatattcccg gagtttttga agcagaaaac ggctttgtcc
      601 tccgtagtgt gacggatgcg gatcgaatca aatcgttcct tgaagaaaaa aatccacaac
      661 atgtcgtcat tcttggtgca ggtgttatgg gtctggaatt agctgagaat ttcaagcatc
      721 gcggcttaga cgtgacttta atcgatcaat tgccacaagt cgctttccct tatgatccag
      781 aaattgctaa tttagtttat gacaaattgc tgaaagaagg attagccgtt catttagaaa
      841 caagagttac tgaaatccgt gataaaggtc gagaaatagt attatcagat ggctccgtcc
      901 tttctgctga tatgctaatt tttgctgttg gtgtttctcc gaagaatgaa gtggtgaaag
      961 caaccggcat acaattatca gatacaggtc agatcattgt tgatgaccag ttacaaacca
     1021 atctgccgga catctatgcg attggcgata ttatcgaaac gactagtgta gtgactggtc
     1081 agccgatcca aagtatgctt tccagtgcgg ccaatcgtca aggacatatg ctggcagata
     1141 ttttaaatgg tacgcctatg cgctatcgcg gatatattgg tgcaggtgtc gcaaaaatct
     1201 ttgatcatac agcaagttat gcgggaatga cagaacatga attaaaagca tcaggcataa
     1261 caaattataa aactgttttt atcactccct ttgaccatgc ctatttctat ccagaagcta
     1321 caagattaaa tctaaagctg atttttgatg cagatagcgg tcgtatttta ggtggacaag
     1381 catttggaga aaaaggtgtc gataaacgga tgggagaact ttctgtagca atcaccggaa
     1441 acttgacagt ctttgatttg cctgatttgg agttgcctta ctctccaccg tattctacta
     1501 cccgtgaccc gttgaatata gctggttatg tcgcaatcaa tcaaatgacg aatatcgtag
     1561 aaacgatcaa agcaagtgat atacccgaaa acgatttgaa agaagcgttc tttttagata
     1621 tacgtgaacc taataaagca ccttctggca gtatctcggc aacaaaaaat attccaatga
     1681 atgagttacg agatcgaatc aatgaaatcc caaaagataa aaaagtttac attactttca
     1741 gaagaggatt gaatacttat acttctgccc gaatcttggc aggtttgggt atcaaagcga
     1801 ttttgattga agaataaaaa aacgaccgcg gtgggtagcc gcggtctagg agttattttt
     1861 acttgaggag taaaaatgaa aaagtatata gggttgttgg ttggtatgta tatagaatag
     1921 aagataaatg tgaagaaatt gtgcattatt tcttcacatt tattaggtta tagtttaaga
     1981 aagtttaaac attgcgcaat ttaattattt tagctgtttc ttttcataaa aaaacatgtt
     2041 gtgactctga gacaaatcat agtccatagt gatgtctcta gagtaactac gcctaatatt
     2101 acgaggcatc atattaatct atcagcacta gtacctaaac tagcatccct ttcgaatatt
     2161 ttttcttata taataaagtt tgctttcact cctctaatct caccaaatcg actggcttgc
     2221 gttgtgtgat actaggaact gattggttta gaacgacttt tctattgtaa tcatattttt
     2281 cgccatcctc gttgtcttct aatccttcaa tgaatgatcc ctaatgtgtg tattaaataa
     2341 tttaattttt ttgttcctat ttatttaac
//
LOCUS       DAZKCY010000215         2355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000215 DAZKCY010000000
VERSION     DAZKCY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.223"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1309
                     /locus_tag="ACG8XC_002791"
     CDS             <1..1309
                     /locus_tag="ACG8XC_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192430.1"
                     /translation="LYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKK
                     VIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLT
                     CALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLV
                     ALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQI
                     FFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINE
                     ETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVAS
                     NKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALM
                     ALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1296..>2355
                     /locus_tag="ACG8XC_002792"
     CDS             1296..>2355
                     /locus_tag="ACG8XC_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFP9192431.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLI"
BASE COUNT          781 a          365 c          403 g          806 t
ORIGIN      
        1 tttatatact tttgcaactt atcgaacagt tgatatctat tatcgatccg caattgcaga
       61 agcaatcgct cttacgatta tcccagctgt tttatattat gcttatggat tgatattcaa
      121 tagagaaaaa aaagtcatcg ctttatctgt cagtataagt ctacttgtgt atactcatgt
      181 cttaactaca ctattgtgta gtgcattgat tgcttgtatg attatagttc gtttgttttt
      241 aagcaagcaa ataaaaaacg atataaaatt tgtggtcgtg aatttagtaa aggctgtttt
      301 tttgacttgt gcattaacta gctatttttg gtatcctatg ttgcagcaga tgaattatca
      361 agaaatacac agtcccttca aaacagattt gcagctagaa gcactttcta tttcagatag
      421 tatcattcaa gcgttaaata acgatattac aacatacact atggggttat taggattggt
      481 tgcgttgatt tttcctttat tcatactgaa agaaatggag aaaaaagaaa aaatcatcta
      541 tgttggcgta gtttctacat gggtatgcgt aactaattta tttccatggt ttttgtttca
      601 aaaaacaccg ataagcttac tccagtttcc ttggcgtatc ttaggtatcc aaatcttttt
      661 ttcttcaatg atcataacgc ttgtttttat gaaaaagact atgaataaaa aatattcatg
      721 gttaatcatt ggcgctactg tggtgtttct actcgtcacg tctatagcag caaaagaaaa
      781 ttacgcaaaa gttatccaat caaaacacgg tcatattgtt atcaatgaag aaacactccc
      841 acgttataca acatcagata tgggaggatt atacgattac gccccaacag aagcgataaa
      901 agaaaggcct cacttagaaa aacatgaagt aaaagtagga gaatcgtggg aaaaagggaa
      961 gtataaagct gcagataata acattactta cacagtggca agcaacaaaa agcaaaaagt
     1021 gacagtacca gtatatgctt atttaggtac acaggtaaaa gtaaacggta aagtcgtaaa
     1081 tgactcttat aaaaaacatg gcttagtgaa tgtggatgta aatgctggag aaaataagat
     1141 agaaattaca acaaaatata ctccaactgc tctcatggct ttatggatca gtattctgac
     1201 ttattgttta gtggtgtatt caagttttaa acgatttgca cctaataagt tgaaagattc
     1261 tcgatatgtg actaaaaagg agataaacaa gaagaatgaa aataaataaa aaagtagtaa
     1321 tattgagtgg attaattata gttagtagta tatattcttt catctttttc aaaaatacac
     1381 agagtattta taccactgga gatctttctt ttcacttatc tcgaatcaag gggttatcga
     1441 ctatttttac cagtccgatt aattatgaga cattcaatta tactggatat ggtgtaaatt
     1501 atttttatcc ctttctgact ttttttccag ctgtaatgtt atattggatg acaaaaaatt
     1561 tgattgtaag ctatattatt tacgtttggt tattgaactt gtgtacaaca ctcgtagctt
     1621 atcattatgg cgagcggttt ttaaaacaaa aaaaagcggc atttttattt agttgtctct
     1681 atattttctc tgcttatcgg acagtagaca tctactatcg ttccgcaatt gccgaagcta
     1741 tcgcaataac acttgttatc cctgtattat tttatgctta tcaaatcatc tctggaaaag
     1801 aagaaaagta tccgtctgta aagctagcat tgagtatgag tttgctagtg tacagccatg
     1861 tactatcaac attaatgagt acagcattaa ttatcatttt tatttttatt cgtttggttt
     1921 ctaaaggttt caagaatgca gattttattg ctatatttaa aaaattattt agtgcggcag
     1981 gaatgacatt ggttctgacg agtgcttttt ggtatccgat gtttgaacaa atgctctacc
     2041 aaaaaatcaa caaacctagc gtgacaaatt tatatgctca tgcatctaat gtgttcgata
     2101 gtctgactga agctatgaac aacgacttaa ctacttactc aatgggacta gttggtctgt
     2161 tatctttatg tattcccctg attttattta aaaaattaac aaacatagaa aaaaagattt
     2221 attatggtac gtgtttgact tggctagcta caactagttt ggtgccatgg tatcttctgc
     2281 aaaatacgcc agccaaattg ttacaatttc cttggcggat cttaagcttg caaataatat
     2341 tctcttctct gatat
//
LOCUS       DAZKCY010000216         2350 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000216 DAZKCY010000000
VERSION     DAZKCY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.224"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..386)
                     /locus_tag="ACG8XC_002793"
     CDS             complement(51..386)
                     /locus_tag="ACG8XC_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192432.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(400..1434)
                     /locus_tag="ACG8XC_002794"
     CDS             complement(400..1434)
                     /locus_tag="ACG8XC_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9192433.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(1524..2204)
                     /locus_tag="ACG8XC_002795"
     CDS             complement(1524..2204)
                     /locus_tag="ACG8XC_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9192434.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT          666 a          489 c          418 g          777 t
ORIGIN      
        1 tacttaaaac aaaaagcgaa gtaaaaaaga aggagcgaaa aaatgaaaat ttattttgta
       61 gcaacatgtt catcctcctt ttcctttctt ctccataaca acaaccacgc aagaagccat
      121 aattctatac caatgatcaa ataaagaaaa cggatctttt cttcattcgt ctccgggaat
      181 acgccatgtt gattagattc tttataaatc ggtagctgtg tctgctgttt tgatgcgcca
      241 ttgactgaac cacttggatg cggaactgga cttagatcat aaaatgtaat cgtacctgaa
      301 cttttggtat ctgcttggac tggctcttgc cagctgaaga acagcaaact tgcaataatc
      361 cctcccaata agatcgatgt tttcaacgaa atcacctcat catgttgttt ctcctttttt
      421 ctttttcttg tggacccaca aaaacagcaa aatcaaccat aacactagaa ctgcagcaac
      481 caatcctgca taaagccatg ggaaagattg ttcttgtact gcaaaaacgg aattttcatt
      541 tactttttgt gctttttctt ttttgatcgt aaagttttgt gtaaatcgcc attcattact
      601 gtcgcctgat tttgctatga tttctgcgcg gtaatcgccg gcttttgctt tatcaccagc
      661 taaatcaaga ttcaagtgaa aattcgaatt tggtgcaact aaaaattcat tagacgtatt
      721 ttcaatataa agctgatttt cataaaagat cttgctcgta aatatcaaat tgctgataat
      781 cctaggctga ggattttgga aacgaagctg aacagttggc tttgcgttga cctcgtccaa
      841 atctcctgat aaaagtttca gatccggttc tatcgtctct tgactttctt gtaaaacgac
      901 tgcgagatta taagcaatac gatttttgat ttgtgctcct tctgtagcat tttctgtttc
      961 agcttcttct ttaggtgaaa catgaatacc cgctaataca cgtcccttaa agctttttgc
     1021 tgggattttc acttctatag gtatagttgt ttctgcatgt gctgatacat tgatttcctt
     1081 tttcgcaatg agcaaaacgt cacggacatc aaacggcaat gtctcatcta acgttgggtt
     1141 tttttgacta taatcgatcg ttccaccatc actcgttacc gccggattga cagagacctc
     1201 aaatgttcgt tcttcatctg aagaattgct gataattact tctagtgtct gattttgttc
     1261 cggtgccact aataaatcaa aataaccgat tgctttatct atttggttct ctggaaatac
     1321 cggtttgatt ccaaagtctc ctgctgaatc ctttgcttgg ctgattgacg gaaaaatgaa
     1381 aaatagactt aaaatagaaa aaaacgaaac aaacagtcgt ttactttgat tcattgtcca
     1441 ccacatcctt tcaacaacaa ctgcggtaaa aaatccttca cctatagtat tttcaccgca
     1501 gtttgttgaa tttatttcat actctatgct actgggccag ccacgaggtt ccaagtcaaa
     1561 tcagccgtga atgttccagc tgctgctttc ggcactttca gtgtgatatt tgctggatca
     1621 taaacagaaa gccatgttcc cagaccttga tcttttgctg ctgtaaatat tggtgtcgct
     1681 gcacttgctt tactcaaccc agttacagtt tgactagttg gtccagtgga ttcgttatct
     1741 gacgtacttt taacagtgga tgttccaaaa tctaattcag cgccttgcaa aacttgtgaa
     1801 ccgtttacaa aatctgaaac cgaaactgtc acgttccacc cttgtgcttg gccatctgca
     1861 cctacacctc gacgatctga tacttggaga taaggtgtat cgttttttga agcgtctact
     1921 tgatacgttt ttgttccgct ttcaatctcg tgtgtaccaa atggcaattc aggaactaca
     1981 tccaatgtta aagaacctgt ctggccagtt gctggatttt ccggatctgc tggctctact
     2041 ggttttgaag gatcagtagg gttaagaggc tcaacggtat ttgttggttc agtaaaggta
     2101 atatctcctt ttgaagtcgc ttgaccctca gcaaaagctg gtgttgccaa gccgcctaga
     2161 agaagtgttg aaagtaataa tgtcccaatc atgttctttt tcataataaa tcactccttt
     2221 ttcttagtga cctaagtata gtagagacag tatgcaaaaa atcctcatga attcatccgc
     2281 ttacttgaaa ttatgaccct ttttgtataa aagagaaaaa aacaataaaa tcgtttacat
     2341 gaatattctt
//
LOCUS       DAZKCY010000217         2323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000217 DAZKCY010000000
VERSION     DAZKCY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.225"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..222
                     /locus_tag="ACG8XC_002796"
     CDS             <1..222
                     /locus_tag="ACG8XC_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192435.1"
                     /translation="NAIITLIVVMVKSIGNITIGMLFIILAAVLIFMIVIWFLNERSK
                     DNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(474..1046)
                     /locus_tag="ACG8XC_002797"
     CDS             complement(474..1046)
                     /locus_tag="ACG8XC_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9192436.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1062..1667)
                     /gene="fic"
                     /locus_tag="ACG8XC_002798"
     CDS             complement(1062..1667)
                     /gene="fic"
                     /locus_tag="ACG8XC_002798"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFP9192437.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(1788..2045)
                     /locus_tag="ACG8XC_002799"
     CDS             complement(1788..2045)
                     /locus_tag="ACG8XC_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP9192438.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(2038..2307)
                     /locus_tag="ACG8XC_002800"
     CDS             complement(2038..2307)
                     /locus_tag="ACG8XC_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9192439.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT          678 a          470 c          331 g          844 t
ORIGIN      
        1 aatgccatta ttactttaat cgtagttatg gtaaaatcaa taggtaatat cacaatagga
       61 atgctgttta ttatattagc agcagtgctg atttttatga tagttatttg gttcttaaat
      121 gaaagatcta aagacaatca acaccgtagg gatatggaga taaaatcaat agagtcaata
      181 gattatttat cgataataac actaaaaaag ccacgttcct aaacgtgact tacgctgaac
      241 gatatgtgtt gtttggtaga cgaacaaatc gttcattata aatatatgat ggtatcctaa
      301 tatagtaagc gtggctagca tacctttaat tactttacct tttacagtta ccatacacat
      361 tttttgagca gaaatcaagt aaataaaaaa atattatatt tcacaacatg ctaatagact
      421 catatctaga acgcattaag tatttacctg tctggttcgc tgagacttct tttttaactt
      481 tcttcaattt tcttctttat tctaaaaagc gtactgcgac tgaaaccggt tatagcttca
      541 acttccttat acgatttacc tgaagtaagc agttcatatg cgtgcctctt cttcggtgtg
      601 attgttgctt tcggacgtcc ttctctgaaa ttaggattat tctttcttgc aaacattttt
      661 ccttcttggg tacgttccac aatcatatca cgttccattt ctgctacact caacaacgtc
      721 cgtgtcacca ttcgccccat ggacgtattt tcaatcgttc ctagattcaa aaccttgatc
      781 gttacgttat catcgagtaa aggttcaatg atctctaatg cttctctcgt gttacgagca
      841 aatcgatcaa gcttcgtaac aactatttca tctccaggct tcagaccagc caacagctga
      901 ttgaattttg gccgatcccg ttttgtaccc gtaaattttt ctgaaaaaat attcttcttc
      961 tctacgccgt tctcaagcaa ctgttgaatt tgttcttttg tactttgaga agcggttgag
     1021 acacgtgcat atccatattt cgtcatttgg tccacctact tttacaaatc atctatcgta
     1081 tactcagaat acccttcata gaaatagctg acatcgattc ctttcatata taactcgcga
     1141 tcgtcaattt tatcagtcag agcattggaa attagatagc gtatttccaa atcgtttatg
     1201 ggacttcgct ccattgcaga aagatagtct gctttatcga ttaagttcca atctacaact
     1261 ttttgcaatt cttcctttaa aatgagatcc agccaaattc tagtacttcg gccatttcct
     1321 tctcgaaatg ggtgagcgat attcatctcc acatatttct tgataatctc ttcaaaagaa
     1381 gtttgtggca tctgatcaat atgctgtaac gaatgttcta aatacataac tggtgcaaat
     1441 cgaaaatttc cttttgcaat attgaccgat cggatttttc cggcaaaatc gtaaatatcc
     1501 gaaaatagaa attcatgaat ttcagataaa cctttaaatg taccaacttc gatttcattt
     1561 atttttccac aatcgtacaa ttctttcgct ttttgtttac tgagtttttc ttcaacctta
     1621 gccaactcaa cttgattggt taatcccaat ttattctcta aaatcatgcg aaatctcctt
     1681 aactttatga cataagttta tgaaacactc tcaaccccat tatatcgcga ttttgtttat
     1741 gtgtcaaaaa ctattaagtt tctgaccctt tccttacttc cgattggtta atagtgatct
     1801 tttgcagaat aaataaatat cgtttcattt tcaactctat atatcagtct atgttcatct
     1861 gtaattcttc tggaccattt tccagataaa tcatgcttta atggctcagg ttttcctaat
     1921 ccagcaaagg gggaacgatc gatatctttt attaacttgt taatcttttt tatattgctt
     1981 ttgtttcctt gctcatgcca ataaagataa tcatcccaag catcatcaga ccaagcctta
     2041 atcatcagat tcaacctcga ttaagtcatg tgttttaaat gcacctttgg agaattgttc
     2101 atctcctcga cgaatttttt ccatgacgta attattagaa agtgttctca acgtttcttg
     2161 catagaatca taatctcttt ttgataatac aacaactgta tcttctacat ctttacttgt
     2221 tacaataagt gtttcagcat cctcattaac ttgtttcata taactacgta aattttggcg
     2281 gaaatttgaa taagctactg cttccattcc tttcacccct tat
//
LOCUS       DAZKCY010000218         2306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000218 DAZKCY010000000
VERSION     DAZKCY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.226"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..2151)
                     /locus_tag="ACG8XC_002801"
     CDS             complement(52..2151)
                     /locus_tag="ACG8XC_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFP9192440.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT          459 a          465 c          401 g          981 t
ORIGIN      
        1 gaataaacgg tgttaaacgg caggctcaca gcctctttca tttgcctaaa attaattgct
       61 cccatttcct ccgaaccatc cagttttaca gttgatggtt acgatgtaaa atggcaaagc
      121 actttctcca atgcctttca tcaatgcaga ttttgttgaa gtggcatata gattataata
      181 gccttcccca ttttcgattc tggctttttc taatctgtaa cctccttcaa cgatatatcc
      241 acgttggata catgtagcaa tcactttgct ttggacacct ggtgcccacg tccatgctgg
      301 attagatgca tcggcaatat cacttgctct ggcagatttg attggctgat tcgctgagtt
      361 tgaagaggaa ggggtcgagg atgccgcttc ttgctgtgct gcttgtgcga ccgcagcttg
      421 ttgagcggct tgagctgcag ctgctgcttg agcggcttgt tttgcttgtt cttcagctgc
      481 ttttttcgct tcttctgctt ttttcttttc ctcttcttct tttttctctt tatcagtgag
      541 catcttatct acttgtttca attgttcatt caactgagca gatagatctg tatttttcac
      601 tttttctact gcttcttttg ccgtcttgta ttggtcgcgt gttgctgaat ccactacttt
      661 tccgtcttta aagactatag ctgttttttc tttagctgtt tgcaattcag catattgatt
      721 tttagcttct gtttgtgctt gttgaatcaa ttttccaaaa tctgtgttat ccgtttcgat
      781 tgcttggatc tctttatctg ctttcaactt gatgtcatct gctagatgat catcgacgat
      841 ggcaggtgct atgaaccact cattcacttt ttggatatct cggatcttag attggatatc
      901 ttggtaagtt gtttctaact ctgaatatcc tttttcattc ttaagactgg ataaatcatt
      961 ttttagtttt gataaatctt gatgaatcat atctgcacga ataaattgat ggtgatcatc
     1021 tgtataaaag gcagcaagtt tctcatgaag ttcttccatt tgttgctgtc tttttgcttc
     1081 tgctgctact tcggcttcta ctttttgatg atggtcataa taagcccagc ttcctccacc
     1141 gagtagaagt actgctgcta aaacgagata aggtactttt tttgattttt tcttttgcgc
     1201 tttatctgga tctgtttttt ctaagtttga ttcttgatgt gttttttcag tatctggcac
     1261 gattgttatt gatttcgctt ctgcttcaaa cagcttaggc tggttctctt ctttttctat
     1321 ttttgcttca agatcagtac cctctttgct tacagattga ttttcagcat cggattcact
     1381 atttatttca tcgggctctg ttgacttttt gtctgtgttt tcgagtattt cttttttctc
     1441 tgcttccggc agtttattcg ttttttcctt ttctaattgt ttttcagtaa atacttcagt
     1501 tttttctgtg atatccgttt ttacgtcttt tatttttact gtttctttac ctgtggcgtt
     1561 ttctctatca gagtcttttt cagtttcttg ttccttttct ggctctactt tgttttcctg
     1621 tgcaattttt tcactgatca ttttgagcag ctctttcgcg tgctcttctt ttgtatcttc
     1681 acgatgtgct ttgatataag cggatagtat cgaattttct tcctcattat cgacgatttc
     1741 tttcagttca gctactcttc ttttttgttt tgattcaagc gtttcttctt gattctttcc
     1801 tgattcttcc gaagtctcat gaacagttgc tttcttctgt tcatttttct ctatattagt
     1861 tacttgatcg tgagcaggtg gttcttcgtc ttctggttct gctttttcga ataattccat
     1921 tacagattct agaggtaaat ctttcaattc cgaccattcg attggatcgt ttaattctgt
     1981 atcaggaaag actgcatctt cagttgattt atcctgcata gctgcatctg tatttgtttc
     2041 ttcaattgat tcttttattt gctgatcttc tgcagttatt tgatgaccgc agtctggaca
     2101 gatagtattc tgctccattt ttttcgatcc gcaatttgga cattttttca tcacttagcg
     2161 atcggctcct ttactaggtt tacgctacca tttaatctat catattctat acgtcacgcc
     2221 aatagcttat ttttaggatt tacttaaaaa taatgaatga atttccttcc tatagaaaaa
     2281 aaagcttgag actgcgtagt ctcaag
//
LOCUS       DAZKCY010000219         2290 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000219 DAZKCY010000000
VERSION     DAZKCY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.227"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..1496
                     /locus_tag="ACG8XC_002802"
     CDS             69..1496
                     /locus_tag="ACG8XC_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9192441.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1588..1953)
                     /locus_tag="ACG8XC_002803"
     CDS             complement(1588..1953)
                     /locus_tag="ACG8XC_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP9192442.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1950..>2290)
                     /locus_tag="ACG8XC_002804"
     CDS             complement(1950..>2290)
                     /locus_tag="ACG8XC_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP9192443.1"
                     /translation="LEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKKNYAERNTRN
                     ADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVISYFLNQEIED
                     KEKQRRGEVG"
BASE COUNT          763 a          434 c          379 g          714 t
ORIGIN      
        1 tttcaaaaag aaaacatgtc ataatatttc ccatattgtg caaaaaaata caaggcggag
       61 gctctactat gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg
      121 ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt
      181 gcaaaaccac tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg
      241 cttatacaca tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat
      301 gcaagatcat gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt
      361 tggaagaaga tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca
      421 aagcttattc attaaaaaaa caagtattag cttactttaa tgactgcggg atcgaaatcg
      481 accgctactc ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt
      541 atcgtttagg cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc
      601 gtttcattga atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa
      661 tcttatcgat tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag
      721 attcaaaatt tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat
      781 gggaaaacac tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac
      841 tattcaatct ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac
      901 agctacacca agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg
      961 aatctcactt caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc
     1021 tgatgcgttc ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa
     1081 atgaggaaca gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc
     1141 agttacctta tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga
     1201 gcggaattct ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg
     1261 ttcattatct gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg
     1321 caccaacaat ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc
     1381 gggtattatg tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc
     1441 ctatttcgat caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg
     1501 ctgtgacaaa aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat
     1561 ctcttgatac acgtcaggct cacaacctca cttattttgg acatctaggg gtccgatctc
     1621 tttatttaaa aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat
     1681 atcctgcaag gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa
     1741 acttagaagt atatagcctc ccaacatctt tttaatagca ttgttctcgc ttactcggtc
     1801 gctttgcact tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac
     1861 accccaaacc aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata
     1921 tggaagaaga aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct
     1981 ttgtcttcta tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt
     2041 gcaagcttca aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg
     2101 actagtactt ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta
     2161 cgagtattac gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga
     2221 acaagaacga tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc
     2281 cattctagaa
//
LOCUS       DAZKCY010000220         2189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000220 DAZKCY010000000
VERSION     DAZKCY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.228"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8XC_002805"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8XC_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(987..1952)
                     /locus_tag="ACG8XC_002806"
     CDS             complement(987..1952)
                     /locus_tag="ACG8XC_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9192444.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKCY010000221         2173 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000221 DAZKCY010000000
VERSION     DAZKCY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.229"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..571
                     /locus_tag="ACG8XC_002807"
     CDS             53..571
                     /locus_tag="ACG8XC_002807"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9192445.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            699..1382
                     /locus_tag="ACG8XC_002808"
     CDS             699..1382
                     /locus_tag="ACG8XC_002808"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFP9192446.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1691..1764
                     /locus_tag="ACG8XC_002809"
     tRNA            1691..1764
                     /locus_tag="ACG8XC_002809"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1725..1727,aa:Arg,seq:tct)
     gene            1923..>2173
                     /locus_tag="ACG8XC_002810"
     CDS             1923..>2173
                     /locus_tag="ACG8XC_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFP9192447.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSML"
BASE COUNT          738 a          335 c          440 g          660 t
ORIGIN      
        1 tatcgttatt atatgattaa gaaggattga ataaagaaag aagttgaaaa cgatacctga
       61 ttacaaaaaa ataactttta taagttctat aacgttgatt atatttttaa ttcttattct
      121 tctcgtcgtc ttggttcccc gaatttttgg tttgactcct tatattattg aggacaaaga
      181 aatgagtcca tattatgcac aaggaaatct gatatatgta cgaccgaaaa atacaaatga
      241 gattttagtc ggagatatta ttacatatta tgaaaattca ggaaaaaaaa tctcgacgcg
      301 tcgcgtaata gccgttgaaa acaaccacga agatttttat acaaaagctg atacaaagac
      361 ttatattgag cctggagtag taaaaaagag aaatattatc ggtagtccca tctttcagat
      421 tccatatatt ggactagtcc tttcaaaaac ggcatttgca tggattcagg ggttattctt
      481 tacgattgct ttttgcctaa caggaatcac agcatggaat acctttgtag agttgaaaaa
      541 gaagcggaga cgaacacaga aatttttgta agaatgttaa aaacataaat gtattccaga
      601 gatccaacga ctcttctgaa catgaaacac gagaaaacac tcatgcagct tgtagattta
      661 gtcagttgtt cgtataatgt gtgaagagga ggagtagtat gcgcaatgaa atgatgcatc
      721 gtcctgtagt caaagaagaa ctattggatt ttatgcgtac ccagcaaaaa aaattaccgg
      781 gtgagctagg caaactggaa gaagaagctc atgtagcaga agtaccagtt attccccatg
      841 aaacagttgt atttttgaaa tttttacttg gacagcttca gcctgaacga atcttggaga
      901 tcggagcagc tatcggtttt tcatcaagtg tcatggccac gacaatcgga gaaaacgctc
      961 atgttacgac aattgatcgt ttcgatgtca tgatcgagaa agctaaaaaa aactatgaac
     1021 gtctaggact gacagataaa gtgactttgc ttgaaggtca agcagcagat atcttgccgg
     1081 agttgtctgg tccgtatgac tttattttta tggatagtgc aaaatcaaaa tatattgaat
     1141 ttttacctga atgcttacgt ttgttaagaa aaggtggcgt tttgatggta gatgacatct
     1201 tccaaggagg aacgatcttg ctcccagatg aagagattcc ccgtggcaaa cgagctatcc
     1261 atcgtaaatt aaatgaattt cttcgtgttg tgatggatca tcccgatttg acatcgacaa
     1321 tcttgccatt aggtgatggt gtgatcttaa tgacaaagga atcagaaaca atcgagttgt
     1381 gatacatgat tatggcagcc tgtagataat cgattgaatc gagggctcaa ttcgaacttg
     1441 atgtaaaagt ttctatattt gtaagtcaat taaaggatac aggaaatcta aataaacagg
     1501 agtagcatga agtcaatgta gctcctgttt tcaattgata accttagaac agctgaagga
     1561 gaatgtctcg ttttaaaaga ttgatttagt agttgggcca aaataattca ttatttgcta
     1621 attaaataac tatttcgtat tgacagaagc aatttaatta tctataatta ttgatgttcc
     1681 gctgattctt gtcatagtag ctcagctgga tagagcatcc gccttctaag cggacggtcg
     1741 ggggttcgaa tccctcctgt gacgctaaaa aaccgttaga aactgaggtt tctaacggtt
     1801 tttctttgtt ttttgtgcca atattgtttg ttttcaaagg caaataaaat aaaaaaacat
     1861 ccttatcgag tacaattata aggagcaaaa aaataaaact catcagaaaa gaggaagaag
     1921 atatgacgat actatctaga attttagtga ctctagttgc aattgaattt ttttatatca
     1981 tgtatatcga aacggtgaga actgattctg acacgacaag tagggttttt aaaatgagta
     2041 aagaagagtt aagcagaaaa tctgttcaaa cattgtttaa gaaccaagga gtgtacaatg
     2101 gactattagg agtgggacta ctttatggag cttatctttc ttccgcatct aaagaaatca
     2161 caagtatgct att
//
LOCUS       DAZKCY010000222         2150 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000222 DAZKCY010000000
VERSION     DAZKCY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.230"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..204
                     /locus_tag="ACG8XC_002811"
                     /pseudo
     CDS             157..204
                     /locus_tag="ACG8XC_002811"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            220..2139
                     /gene="tet(M)"
                     /locus_tag="ACG8XC_002812"
     CDS             220..2139
                     /gene="tet(M)"
                     /locus_tag="ACG8XC_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFP9192448.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKINIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     HPNMRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          716 a          341 c          445 g          648 t
ORIGIN      
        1 tcaatttgat agcgggaaca aataattaga tgtccttttt taggagggct tagttttttg
       61 tacccagttt aagaatacct ttatcatgtg attctaaagt atccggagaa tatctgtatg
      121 ctttgtatgc ctatggttat gcataaaaat cccagtgata agagtattta tcactgggat
      181 ttttatgccc ttttgggctt ttgaatggag gaaaatcaca tgaaaattat taatattgga
      241 gttttagctc atgttgatgc gggaaaaact accttaacag aaagcttatt atataacagt
      301 ggagcgatta cagaattagg aagcgtggac agaggtacaa cgaaaacgga taatacgctt
      361 ttagaacgtc agagaggaat tacaattcag acggcgataa cctcttttca gtggaaaaat
      421 actaagataa acatcataga cacgccagga catatggatt ttttagcaga agtatatcgt
      481 tcattatcag tattagatgg ggcaattcta ctgatttctg caaaagatgg cgtacaagca
      541 caaactcgta tattgtttca tgcacttagg aaaataggta ttcccacaat cttttttatc
      601 aataagattg accaaaatgg aattgattta tcaacggttt atcaggatat taaagagaaa
      661 ctttctgcgg aaattgtaat caaacagaag gtagaactgc atcctaatat gcgtgtaatg
      721 aactttaccg aatctgaaca atgggatatg gtaatagaag gaaatgatta ccttttggag
      781 aaatatacgt ctgggaaatt attggaagca ttagaactcg aacaagagga aagcataaga
      841 tttcataatt gttccctgtt ccctgtttat cacggaagtg caaaaaacaa tatagggatt
      901 gataacctta tagaagtgat tacgaataaa ttttattcat caacacatcg aggtcagtct
      961 gaactttgcg gaaaagtttt caaaattgag tattcggaaa aaagacagcg tcttgcatat
     1021 atacgtcttt atagtggcgt actgcatttg cgagattcgg ttagaatatc ggaaaaggaa
     1081 aaaataaaaa ttacagaaat gtatacttca ataaatggtg aattatgtaa aatcgataag
     1141 gcttattccg gggaaattgt tattttgcag aatgagtttt tgaagttaaa tagtgttctt
     1201 ggagatacaa agctattgcc acagagagag agaattgaaa atcccctccc tctgctgcaa
     1261 acgactgttg aaccgagcaa acctcaacaa agggaaatgt tacttgatgc acttttagaa
     1321 atctccgaca gtgacccgct tctgcgatat tatgtggatt ctgcgacaca tgaaatcata
     1381 ctttctttct tagggaaagt acaaatggaa gtgacttgtg ctctgctgca agaaaagtat
     1441 catgtggaga tagaaataaa agagcctaca gtcatttata tggaaagacc gttaaaaaaa
     1501 gcagagtata ccattcacat cgaagttcca ccgaatcctt tctgggcttc cattggtcta
     1561 tctgtagcac cgcttccatt agggagcgga gtacagtatg agagctcggt ttctcttgga
     1621 tacttaaatc aatcgtttca aaatgcagtt atggagggga tacgctatgg ctgtgaacaa
     1681 ggattgtatg gttggaatgt gacggactgt aaaatctgtt ttaagtatgg cttatactat
     1741 agccctgtta gtaccccagc agattttcgg atgcttgctc ctattgtatt ggaacaagtc
     1801 ttaaaaaaag ctggaacaga attgttagag ccatatctta gttttaaaat ttatgcgcca
     1861 caggaatatc tttcacgagc atacaacgat gctcctaaat attgtgcgaa catcgtagac
     1921 actcaattga aaaataatga ggtcattctt agtggagaaa tccctgctcg gtgtattcaa
     1981 gaatatcgta gtgatttaac tttctttaca aatggacgta gtgtttgttt aacagagtta
     2041 aaagggtacc atgttactac cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg
     2101 atagataaag tacgatatat gttcaataaa ataacttagt gtattttatg
//
LOCUS       DAZKCY010000223         2077 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000223 DAZKCY010000000
VERSION     DAZKCY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.231"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..498
                     /locus_tag="ACG8XC_002813"
     CDS             <1..498
                     /locus_tag="ACG8XC_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192449.1"
                     /translation="FRLKTKKESVIILVYFQGGNLMIKKLFLLSILTLAMAGCSDSGL
                     ATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKI
                     KRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKT
                     YDILA"
     gene            complement(559..924)
                     /locus_tag="ACG8XC_002814"
     CDS             complement(559..924)
                     /locus_tag="ACG8XC_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFP9192450.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1000..1245)
                     /locus_tag="ACG8XC_002815"
     CDS             complement(1000..1245)
                     /locus_tag="ACG8XC_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9192451.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1315..1569)
                     /locus_tag="ACG8XC_002816"
     CDS             complement(1315..1569)
                     /locus_tag="ACG8XC_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192452.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1733..2056)
                     /locus_tag="ACG8XC_002817"
     CDS             complement(1733..2056)
                     /locus_tag="ACG8XC_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192453.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          772 a          358 c          369 g          578 t
ORIGIN      
        1 tttcgtctga aaacaaaaaa agaaagcgtt attattttag tctattttca gggggggaac
       61 cttatgataa aaaaactatt tttactatca atattaacgc tagcaatggc aggttgttct
      121 gactctggct tagcaactga actgatcaca aaatcaactg aagccacgac agaggagtta
      181 aaatgtggaa acgatcacag tgactacaga ggcttaaatc gacgatgcat gggaatatta
      241 ggcagaagca attgtaagca tcgatgtagt aatcgaagaa ggtgtagaag aagcaacaga
      301 aaaatcaaaa gaaagatcaa aagaagcaat ctgtgctgtg catcgtatac agacgtcgaa
      361 gatgcggtac ttttaaacca cgtcaggaga tgttcattta tgtctaggga agttggcgat
      421 gaaccaaaac tagagtacgc ttttagtttt gcacgtgagg atgaagataa ccctaaaact
      481 tacgacattt tagcttgatc taagatggtt aaagcttttt aatgaagtaa gaggagatag
      541 aaggcttttc tagttatatt aggtaagata aattcctttt tgctccaaat ttgctaaaca
      601 ttctgctaga taaacttcat cgtgttccgg ataaatcggc acgttgcgct ctacagtagg
      661 taaatcaaaa aatgcttcgc atgatttgcc acgatacata ggatttttcc actctggtgc
      721 tgggctgtaa ccacgttttt ccatttcatc catgacaagc tgatgatatt ggaataatct
      781 ataaggagaa tagtgaaaaa cataatccac agtagcatgt ttctttcccc agccatttcc
      841 tcttaatgca cagcattccc gatgttgtcc taaaagctgt tgacgaggaa gctttgaaat
      901 caatttttca tgccataatc gcatagaaat gtctccttta tatatagatt gttaacctta
      961 gacgttaaat gaaaaaacca gcaccaaggc tggaaaaaat cacttcagtt tgcttgctgt
     1021 ttctgagcga ccaattaggt aatcaacgga aatatcgtaa aagtcagcga tatagattaa
     1081 ttcaatgatg cttggaattt cttctccttc ttcgtactta cgatatttga ataaaggaat
     1141 acttaacgcg ctagaaacag ctacttgaga taaagatcgg ccagttctta agaaattcag
     1201 acgacaacgt aaagtatcca gtaaaatagc ttcttcgata ctcatttaat cacctttcat
     1261 tatagtagaa tagttataat gactatagaa agctacacat gcaatcaata gtactcaatg
     1321 ttttttattt aaacgtatca aataaagaat gacgatcaat aaagacaaaa ccagtcctga
     1381 taattcttct ataaaaacac tatcgggttc aaagtagttg taattaaaaa ctttccacat
     1441 aaagtagata gccggaagag tcaaaatagc taaataaaac cgttctttgt tccaattcat
     1501 aagataaaca atacttgcga ccagtgcgac cgcaggcacc caaaacaaaa ctctcaccaa
     1561 cgtaatcatg taacggactc ctcaacgatt catataataa aagataacat gttattgcat
     1621 acatcacaaa tgaaatgcta ggaaaataaa agaagaatag atcaatgaaa aatgaaaaag
     1681 cagaacagtt ttctgctgat aagcaggaac tgttccgctg aatgtgtctt atctatcgag
     1741 aaaaaatcaa tggattgata tctaagtaat tcaatacata taaaaagagg acgatcgcat
     1801 tcactaaaaa ggaaagaatg atgatcccat agcgaatgga aacatattta acaaatactt
     1861 ttgtttgaat acgggcatat ctttttcctt tatagctgag catcatgaca agaagcaaaa
     1921 gggtaatcac accttgaaaa atcattccaa taaacagaat tcctgaggtt tgataattta
     1981 attggagaaa ataagctgct gccgcaacta aatcgaataa aatcgcaata aatggcaaaa
     2041 taatcactcc taacattgag aataaataaa agaataa
//
LOCUS       DAZKCY010000224         2076 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000224 DAZKCY010000000
VERSION     DAZKCY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.232"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>2076
                     /gene="acm"
                     /locus_tag="ACG8XC_002818"
     CDS             <1..>2076
                     /gene="acm"
                     /locus_tag="ACG8XC_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFP9192454.1"
                     /translation="NITSLIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSF
                     DEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNEN
                     IKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYY
                     KTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQK
                     VYRGEEGIQQFLQDFPGATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQIN
                     FENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPN
                     VTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSN
                     ELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPV
                     PDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVV
                     TNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTN
                     LPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEK
                     TEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSF
                     AVIIGLLIVT"
BASE COUNT          814 a          342 c          407 g          513 t
ORIGIN      
        1 taatataaca tcactgattc ctgttcatgt ctatgcggat gcaggcagag atatcagcag
       61 taatgtcact tcgttgaccg tggacccaac gaatattaca gatggaggaa acataaaagt
      121 aaaattctcg tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc tttggatcaa
      181 ttggccaagt gaaggaaata ttcgcggtga aggattccaa aaagaaatcc cattaatgat
      241 tgaaaataaa aatgttggaa ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa
      301 tgaaaatatt aaaaacctag actcagtaga aggttggggg caatttgaga tacaggccag
      361 aaacgtaacc gataccggtg aagaaaatac gggaccattc accgtgacga gcggtgataa
      421 aacagctact gtaaatgtga caaagccagc ttctggttct tcttctagcg ttttttatta
      481 taaaacagga gacatgctac ctgaagatac taagcacatt cgatggtttt tgaatattaa
      541 caataacggc acttatgttg aacaaccagt taaaatcagt gatgaaatcc aaagtggaca
      601 aagattagat ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac agaaggttta
      661 tagaggagaa gagggaatcc aacaattttt acaagatttc cccggcgcta catttaattt
      721 ttcagtaact gataattaca ttgaaataac tataccaaaa aactttgtca atcttaggaa
      781 aataatggtg agttataaga cgatcataga aaacccagaa caaataaatt ttgagaatca
      841 ttcggaagct tggtttaaag aatttaataa acctgctgtt gatggagaaa gtttcaatca
      901 tacagttaaa aatatttcag cttctggtgg agtcaacggt acagttagag gcgaattgaa
      961 gattttcaaa tatatcaatg acacagagat tggtatccca aatgtgactt tcgaattaag
     1021 acgagcagat gaacagccta ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca
     1081 gggagaagct agtatcaaag gcctgcaagt aggagactat gttgtaaaag aaaaagaagc
     1141 acccaattgg attgattttg atccactaag ttcaaatgaa ctcaaattct ccataaacga
     1201 aaatgataca gagggagtca gtttacctat ttataacaag aaaaaggtta cgaacatcac
     1261 agcaactaaa atttggaacg gtggaaccac tccaagaccg agtatttatt ttaaattatt
     1321 cagagcaagt acaaacaatt gggaaccagt tcctgatgca gaaacaaaac gattagacaa
     1381 tggaataact tcagtgactt gggaagatat ccaacagtat gatgatagtg gaaacgaata
     1441 tacgtttaaa gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt caggttatca
     1501 aaaaattgaa aacgggctag tcgttacaaa tgagaataaa gaagtcattt cagtctcagg
     1561 acaaaaaaca tgggaagaca atgagaacca agacggcaaa cgaccgacga cgatcacagt
     1621 aaatctatta gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa aagatgattg
     1681 gcgctatcgt ttcacaaatc tgccaaaata cagagacggg caagaaatca tctatactgt
     1741 aacggaagac aacgtgcccg aatacagtac gacgattgac gggtataaca tcaaaaacag
     1801 ttatacaccg gaaaaaacaa atatatctat accaggtagc catataacac cgtggaaaaa
     1861 ttttccagag aagacagaga gagagaaaat tagaaatcta ttctctcaaa aattgcaaat
     1921 atttgctcat acaggagaat ccagcgaaca aagaagcaat tatattagta agagagctgt
     1981 acctaaacag ataacaaata aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc
     2041 tagttttgct gtaatcattg gtttattgat agtaac
//
LOCUS       DAZKCY010000225         2075 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000225 DAZKCY010000000
VERSION     DAZKCY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.233"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(491..1783)
                     /locus_tag="ACG8XC_002819"
     CDS             complement(491..1783)
                     /locus_tag="ACG8XC_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9192455.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          657 a          402 c          335 g          681 t
ORIGIN      
        1 atattataaa ttcacatgat tatcgaattt tattaagtta gtttttcttg taaatctctg
       61 ttcccaatga aactcgagac acttatttta aagaggaaac aatgtggtat ttaaatagcc
      121 ctcttgtatg ggctactctg tgatacttat tagccattaa actattttac ccacatagca
      181 tatattttga aatcgttcat tccgtaaatt caactaaata tgcctaaaaa aataaaaaat
      241 ctaagataga ataagattta ttcagtctta gatggattta tagcaacttt ttcgtaacaa
      301 ctttaaaaaa tacgaaaaga aagagctgtt ttactctgac tacgttgcta aattgtcgtt
      361 taatcattca tttaaaccaa atcttttttt atagaattcg ttttaatcct cctgaatcca
      421 aaaactatga aaaccgtaat taaatagtta agagttttta accaccataa ttctctcggc
      481 tattttgaaa tcagataaat tttgatcgga cacatagttg aataaaccta ccgttgttac
      541 ggaaggtaaa tcgcatactt ttcattctag aggagaaggt ctcgttgatc attaactgat
      601 taagcaccat agcccagttt tgaattcgac cacctgacca cttcgcgtgt aattctttcg
      661 tgcgtaaata aagtagtttt aggagtgcat tctcattaga gaacgctcct ttttttgtga
      721 cttttctgaa gctggagtgg acactttcaa ccgcattggt agtgtacata atttttcgaa
      781 tggcactacc ataatcaaat agttgttcaa catgtgcaaa gttccgtttc cagacatcta
      841 cagcaccaga ataatgagac caccgatttt gaaaactgtc aaaagcagca tgtgcagcgt
      901 ttagagaaga agcaccgtag aactttttca tatctcggca gacttcctta tagtccttac
      961 ttggaatata gcgcaatgca tttcgaacaa gatgaacaat acagcgctga acaattaccg
     1021 atggaaagat cgcttttgcg ccttcttcga ggccagaaac accgtccatc gaaatgaaaa
     1081 agacatcttc gacaccacgt gctttcagtt catcaaatac ttgcatccag cgatttttag
     1141 attctgtttg atttaaccat aatcctaaaa tctccttatt tcctttgaga tcatagccaa
     1201 gaatggtgta tacagcatat tctttggctt cataattttc tcgtaaagta acatacatac
     1261 aatcaacgaa tagaaaggca taacactttg ctagggggcg ggcttgccat tcttccaatt
     1321 caggaaggac agcgtcagtg atatctgaaa tcatttcatg ggaaatatca aagccataaa
     1381 tagcttcgac ggttgcggca atatctcgtt gactcattcc tcgtgcatac atggaaagaa
     1441 ccttcccttc gatgtcggag acatctcgtt ttctcttagg aattaactct ggttcaaagg
     1501 aagcttcccg gtctctagga acatcaatag ctacttcacc aaaactggtt ttaagcgttt
     1561 tagttccata gccatttcga cggttatcgt gttccttagg ctctttagaa tgggcatcat
     1621 aacctaaatg attattcaat tctccttgaa gcattttttc aaaaaggggc ccaaacacat
     1681 ctttcaaagc atcttgcatg tcatcgacag attcaggttg ataggcattc agaatggatt
     1741 cagctaactt ttcggcatca ggatttcttt tctttctagc catcgtgtaa tcacctcttg
     1801 atcttattgt agaaaaagaa aaaccgcgaa gcaaccacct gcctaggatt aatggttact
     1861 tacacggttt atattacact ctcatcatta aaaaagtatc tttcgatatt actaaacaat
     1921 gaaattatct atttgaaatt taaatctgat aatatattga gtgttccaat gagggctttg
     1981 atcctattat ccatatcttt ttcagtttta atattgatac acattataag aaaaagaaac
     2041 ttttggttaa tttatttcat cccagtgcaa aatat
//
LOCUS       DAZKCY010000226         2069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000226 DAZKCY010000000
VERSION     DAZKCY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.234"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG8XC_002820"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG8XC_002820"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP9192456.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACG8XC_002821"
     CDS             complement(1172..1867)
                     /locus_tag="ACG8XC_002821"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFP9192457.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..>2069)
                     /locus_tag="ACG8XC_002822"
     CDS             complement(1880..>2069)
                     /locus_tag="ACG8XC_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridylate synthase"
                     /protein_id="HFP9192458.1"
                     /translation="VLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLGVG
                     EYRSLTLAEMKKLSIYFK"
BASE COUNT          579 a          441 c          349 g          700 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaacc
//
LOCUS       DAZKCY010000227         2052 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000227 DAZKCY010000000
VERSION     DAZKCY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.235"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..199
                     /locus_tag="ACG8XC_002823"
     tRNA            126..199
                     /locus_tag="ACG8XC_002823"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:160..162,aa:Ile,seq:cat)
     gene            complement(584..1081)
                     /locus_tag="ACG8XC_002824"
     CDS             complement(584..1081)
                     /locus_tag="ACG8XC_002824"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFP9192459.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1121..1930)
                     /locus_tag="ACG8XC_002825"
     CDS             complement(1121..1930)
                     /locus_tag="ACG8XC_002825"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9192460.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          605 a          371 c          358 g          718 t
ORIGIN      
        1 aatagttatg acacaagtaa aagaaataag aataacaata ttattttact tatattttga
       61 aacttgtgag agtttgtcat tcgtgctata gttgaaaagt aacaaaaata aattttattt
      121 atgatgtacc tttagctcag ttggttagag cagacggctc ataaccgtcc ggtcgtaggt
      181 tcgagtccta caaggtacat aagaggttgt gaaaaagctg ccctgcatca agtaataaga
      241 acagccaagt aaaaacagct tcttatgttt ttacttggct gttcttatta ctgcagatgc
      301 aagcttttga ataccgttta aggaagaggt tgtgaaatca gcgttttgac ctgagtatta
      361 gcttaacaaa cggaaaaata gttcctcata ttttgccgtt tgttaagcta ataccgaggg
      421 tcagctgatt gaacaccatt taagaaagag gtcgtgagcc tgccgtttca ctctgtgtca
      481 taaaaaagcc aatctacttt gatagaaaaa gcacgtagga ctaaaacagt tctgcgtgct
      541 ttttttattg tgtgttaaat atcgtaaatg aagatagata tttttatgat tcgacgatag
      601 agacgttgaa atctttcaac agctctaaag cggattctcc gtgttttcta ctggagacta
      661 acattggatc aacaaaaaag tctgcttcag gaaaagcact ctgcaggcct atcagattag
      721 agagtacaca caagtttgtt tcaatcccga ctacttcgat ctctttttgt tcttctttgc
      781 tttcaaataa agtttcctgg atttcagcta aagtttcagg tggaatcgta aagtaatact
      841 ttttaatcac tcgttcattt tcttgtggag aaagaagagg gatcaattgc aaatcttctc
      901 gttcttcttc tttgtccttc agttcaactg gtatatcccg tgtgaagaga atatattcat
      961 tattttggcg tgctttagct aatctttgag caatacgttc tgccaattga tctgactctg
     1021 gaatataaaa ttcgctgttt ggatcaagaa tatgattttg catatcaata ataactaaca
     1081 ttttctttcc tcctacattc atatcgtcat tttattcttt ttaatagaag ttttttaaaa
     1141 tataagcaac accagcttct tcattcgtca gtgttacttg gtttgcaatt tcttttactt
     1201 gtcgctgtgc attccccatc gcaatactca acccgctgta attcaacata gcaatgtcgt
     1261 tttcttgatc gccaaaagcc atgatgtcac tcgattcgat tccaaggtaa tccgccagaa
     1321 aaacaagtga atgagcttta gtgatacctg taggcgtgat ttctaagaaa tgcggatcag
     1381 agaatactga ttggatacca tctgcttgca gatctctttg tagcttctgt agtttttctt
     1441 cactcttatc tgttaatgct aatttaagat agctaagaga aggatcttcc aacatattta
     1501 aaatttgatc aaaagaatgg ttctgtaaat tgaaatgttc ataataaggg tcatctttga
     1561 ctagatttga ttcatcgaat gtcagctgat catcttgggt ttctcggcta gcaatgatgg
     1621 atattttcgc ttcttgagca tagaagaagg cttttctcag tgtatcgcta tctatttttg
     1681 aacgtctgag aactttcgcc atttttccat ccttcatttc agcaatcagt gaaccattgg
     1741 aagaaatcaa atagccaccg attcctaatt cggaaaaaat atctttagca gaaatgctgt
     1801 tccttccggt agcgacggtt acgattccgc cattttcatc aaatttccga agtgcttgga
     1861 tattttcttc tggtatcttt ccatttcgat caagcaaggt accgtctaaa tcaatcgctg
     1921 ctagtttcat tttatcttcc tactttcttt ttacatatct attttcctgt taccttaaga
     1981 ttaaatgaga atttaaaaaa atgaaactaa cttgctttgt cattatgaat tttttcaaaa
     2041 aacattctta ag
//
LOCUS       DAZKCY010000228         2027 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000228 DAZKCY010000000
VERSION     DAZKCY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.236"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..586
                     /locus_tag="ACG8XC_002826"
                     /pseudo
     CDS             60..586
                     /locus_tag="ACG8XC_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            741..1316
                     /locus_tag="ACG8XC_002827"
     CDS             741..1316
                     /locus_tag="ACG8XC_002827"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP9192461.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1359..1568)
                     /locus_tag="ACG8XC_002828"
     CDS             complement(1359..1568)
                     /locus_tag="ACG8XC_002828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192462.1"
                     /translation="MICLLKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDV
                     LLRFNGFLGEDYRYLKISDPQPLTE"
     gene            1522..1725
                     /locus_tag="ACG8XC_002829"
                     /pseudo
     CDS             1522..1725
                     /locus_tag="ACG8XC_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          717 a          321 c          379 g          610 t
ORIGIN      
        1 gaagtccctt ctgtagaata gagttaccac aacacattca cagaaaagag gacttccata
       61 tgaacgattt tactacagaa atcctaaaga ctctagcgaa caaaggcgat ttgaatggat
      121 tattccgtgt ccatttggaa aaagctgtca atacgcttct caaaacggag ttaacggctt
      181 tcctcgatta cgaaaagtac gatcgcattg gttttaacac gggtaattct cgtaacggct
      241 cctatgaccg tacggtcaag accgagtatg gggaacttca tctccagatt ccgcgcgacc
      301 gcaatggcga gttcaagcaa cagactgttc ctgcttatag acggacgaat gacacattag
      361 aggagaccgt cattcacctc ttccgaaaag gtattaccat gtcggaaatc gcagacttga
      421 ttgagaaaat gtatgggcat cactacacgc cccaaaccat gtccaacatg actaaagtgc
      481 tgactgaaga agtaaatgca tttaaagtta gaactctaaa tgataagtat gtcgctattt
      541 tcatgattcc ttcagttttt cagatgatat accactcaaa aaacaatatg agagatgttc
      601 aggaatacca tctctaatgg cttataaatt ggttttaggg atagtagatg ttatagacat
      661 atcaaggaat aaacgagcag ctgcataaaa gcagatggaa ttaaggaata attgaattgt
      721 aaaagaaggg aataaattta atggcacaat tattttacag atatggaaca atgaatagtg
      781 gaaagagtat tgagctactg aaagtggctc ataattatga agaacaaggg aagagagtta
      841 tcataatgac aagcgcaata gatactcgat caggatttgg aaaagttgag agtcgtatag
      901 gtgtttcgaa accagctatt gcaattactt ctgaaatgga tgtgtacgga gaattagtgg
      961 agattattga tcagtacgga aaagttgatt gtattttggt cgatgagtct caatttttaa
     1021 gtagagagaa tattattgat ctatcactta tcgtaatgaa tgaggaagta ccagtaattt
     1081 gctatggatt gaagaatgat tttcaaaata atctcttcaa gggatccgaa gcattattga
     1141 catttgctga taaaattgag gaaattaaaa ctatttgcca tttttgcaat aaaaaggctg
     1201 taatgaatgc tcgtattgtt gatggtattc cagtttattc aggtgaacag attgtagtag
     1261 gaggtaatga agattacctt tcggtatgtc gcaaccacta ctacaacgaa gattgaaata
     1321 aaaaacgtgt aatcaaataa ttgattacac gttttttatt attctgttaa aggttgagga
     1381 tcagaaattt ttaggtatct ataatcctca cctaaaaatc cgttaaaacg aagtaaaaca
     1441 tcatgaccat ttaaatcagt tggaactaaa attggaatta atttatcaac agtagttttg
     1501 ttgtaaagtt caaaagtgtt ctccattcct tctggttcta caatcgaatc tttcaataaa
     1561 caaatcaaga aatacagccg cagaaaagag cagtttcaaa atgaagaatc actagagcgt
     1621 ttcctagtat ccatctttga tacatacaat caaaaattct taaatagaag ccataaaggc
     1681 ttccaacaag tgacggatac attagcttca atgtttactg agtaacccat tattttgcag
     1741 aaggacgagt tatttacaca aaattattga cgctccctgt ttggagtggt aaaaagattg
     1801 agaaattgtt tcattagcta ttacgattat tattgtaata tatttttatt gaaaatcaaa
     1861 attaaaataa ttcactgtaa aaacaggatt attttttcta ttttggtaag tcaacaggaa
     1921 catagttttc aattgattca attgactcat tttcaataat ctgatccata aatccagggc
     1981 aaatattacc agacaaatta taaaacctgc tttcttcttc agtcact
//
LOCUS       DAZKCY010000229         1970 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000229 DAZKCY010000000
VERSION     DAZKCY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.238"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(68..418)
                     /locus_tag="ACG8XC_002830"
     CDS             complement(68..418)
                     /locus_tag="ACG8XC_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192463.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            complement(411..1736)
                     /locus_tag="ACG8XC_002831"
     CDS             complement(411..1736)
                     /locus_tag="ACG8XC_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP9192464.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
BASE COUNT          559 a          408 c          303 g          700 t
ORIGIN      
        1 taaccccaaa tctcttctga tagctgatca aaggttatat actttagctc atattgctgg
       61 ataatgttca ttctaatcga ccccatttat tatcctggta aaattcgata tgacgtattt
      121 cgtcgtagtg aactttgttt tttccaacgt agatccccag ctcatcacta cctaagatct
      181 tgctaacaat atcttctgga tagtatcctt cagcgtttcg ttcttctttt tgaatacata
      241 ctgatctatt ttttatgact gcagcgttca atacttccgc aacctcttca gtcgtcattt
      301 gttcttttgc caaattttga ttggctcgtt gttcattgtc ggcatcaatt ttttcagtat
      361 gatcagataa ataaaaacct gcccacttta aacgtccacg atcattataa tcaatcattt
      421 ttaagccctc ctgcgccgcc tgcatggcca ccaaccaatg tacttctagt aatactacga
      481 gcgccttcta acatagaaga agcatgtaca atcgacgtaa aaccatattt ttgccgaatc
      541 ttgtcaataa ttttatctaa attttcttcg tcaatttgct tttgtggatc actaaataaa
      601 tccagctgaa gcgaatcagt gtacacaagc ttgctatagg ttattccaac gtgccttact
      661 tcttgtcctt cataatactt atcaaacaag aaaagacagt aattctgcaa ttcctttgta
      721 ttatctgtta tagggatctt catctggtgt gaaaaacctg gtctcgtttc taagatcgac
      781 gtgccaatgt ataaatggac acaacctgtt ttacagttat gttgcctgat acgaatagct
      841 acttgttccg ccatttcttt taccaccagt tcgatttcat ttctcctggt ataatctcgt
      901 ggtaacactt gtgaattacc atatgatttt tcctttgtcg gttctggtgg gatcgctata
      961 tctgttcgat cgatcccgtt cgcatgaaaa tacagctgta agccaatcac acctaaatgg
     1021 ctcttaatag tgtatggatt ccaattagct aagtctttaa tactcatgat accaatttgg
     1081 ttgagtcttt ttttcattcg agatccaata ccccaaaggt ctgtcatttc aggaatattc
     1141 catactttat caggaacatt ttgatacgtc cattcagcaa ggaatccatc attatgtttt
     1201 gcttcattgt ctaaagcaag tttagctaat aagggattat ctccgacacc tactgtagca
     1261 agcaatccta gctcttcttt aatattctcc tggatcattt gagccagctt ctttctccgt
     1321 tgacttcttg ttccttcagt cgtaaaaaga ttcattgatc tggtcacttt taagatcgat
     1381 tcatcgatcg agtaaatcaa taaatcttcg tctgctacat accttctgaa aatattgttt
     1441 acctgcatat ttcgcttaat atagagattc atgcgtggtg gaacgacatg taacgtttta
     1501 ggaaaaggtt gcggtagatc tcgtggtcga ctgacattag aaataccgta cttcttcttt
     1561 gcagctggtg aagaagctaa aatcaaccct gatcctgtat tatccgctcg actcatgacg
     1621 actaattcag tcgttaatgg atctaaattc ctttctatac attcgacact tgcgtaaaag
     1681 cttttcatat cgatcaaaaa gtaatcattt actggctctt ttgaataatc catcataaat
     1741 aacactccat tcttccacat tatacaaaca tacgttcgtt tttgtaaaga agaatcgatt
     1801 aaatgtacaa aaaaagaagc ataggtaagt tatttcccta cttcttctgt tgatttgtaa
     1861 aatcaacaca ttgtatcact ttattaaatg gatatttact attagtcttg tgaaaatttg
     1921 aaaataagtg tcgtattatc cagttttgct tagttatatt agaaaaaaat
//
LOCUS       DAZKCY010000230         1968 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000230 DAZKCY010000000
VERSION     DAZKCY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.239"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            419..688
                     /gene="rpsN"
                     /locus_tag="ACG8XC_002832"
     CDS             419..688
                     /gene="rpsN"
                     /locus_tag="ACG8XC_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9192465.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            703..882
                     /gene="rpmF"
                     /locus_tag="ACG8XC_002833"
     CDS             703..882
                     /gene="rpmF"
                     /locus_tag="ACG8XC_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9192466.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            914..1291
                     /locus_tag="ACG8XC_002834"
     CDS             914..1291
                     /locus_tag="ACG8XC_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192467.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1312..1461
                     /gene="rpmG"
                     /locus_tag="ACG8XC_002835"
     CDS             1312..1461
                     /gene="rpmG"
                     /locus_tag="ACG8XC_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9192468.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1485..>1968
                     /locus_tag="ACG8XC_002836"
     CDS             1485..>1968
                     /locus_tag="ACG8XC_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9192469.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     Q"
BASE COUNT          718 a          282 c          394 g          574 t
ORIGIN      
        1 aatcagtatg attgaattag ttcacttaat gtctattctt ataatataag gaataatcaa
       61 agttttaaaa aattggcgat aaaccagtta aacataaaag attgaataaa aagttatagt
      121 ttctttcata ttgccgtttt aagtagagga aaagctactt aaaacggtat ttgtgtttga
      181 ttggactaat aaaagtgaat tcttattttt cagagcctaa actgcatatt cttttaaatg
      241 atttgattat ctctatgtac cgataaattc gttttcaaat agttatctat tcatatgagt
      301 tttatttcat gttatttatt gttgacaaga gcaactcttg ttggtaaagt atcagtgtca
      361 aaacgtaacg attacgattt gaggtgtcta aatcaaaata aaaggagtat aaaggtatat
      421 ggcaaaaaag tcgaaaattg ctaaagcaaa gaaacaaatg gcgatgattg aaaagtatgc
      481 agataaacgt caggaattaa aagccgcagg agatcgcacc gctctcgcta aattgccaag
      541 agattcaaat ccgaatcgtc tgaggcttcg tgatcagacg gatggtcgtc caagagggta
      601 tatgcgaaag ttcggtatgt cccgtattaa gtttagagaa cttgcccatc aagggttgat
      661 tcctggagta aaaaaagcaa gttggtaaaa ggaggaaata tcatggcagt accagcaaga
      721 aaaacatcta aggcgaagaa acggttaaga agaactcatc aaactgtagt aaaaccagaa
      781 atttcatttg acgaagcgaa tggtgattat agacgtagcc atcatgtatc tttaaaaggg
      841 tactataatg gaaagaaagt aattaaagga gcttcaaaat agtttaatat ataactttta
      901 ggagggaatc aaaatggaaa ttatttaccc acctttagtt gaagaaggat taaagtatta
      961 tcttgaaacg acccagcaat cgctagataa atgcactttt tatcgttcga tggtagagag
     1021 aggaattatt actgaaacgg gtttaccgac ccaacaggca attgaaaatg gattagtcaa
     1081 agattactat gaggaccaag gcttatcatt tgatgatttt ttaagaatct atccaatttt
     1141 tgaagagtat gacgaggagt tatttcaatg tatcgatggt tattgggaga tacctgttga
     1201 tatgaaagaa aatttagttt cacaattgga atctggcgag ttaacttttg aagatgcaca
     1261 acaaattcaa gcatatcttg aagatcgata agtattgaag gaggaagatt tatgcgtcaa
     1321 aatattattt tggaatgtgt cgaaactgga gaacgtctgt atctcaccag caaaaataaa
     1381 cggaataacc ctgaacggtt agaattaaaa aagtattcgc caaaattgcg cagacgggca
     1441 atttttaaag aagtgaaata gctaagtggt aaaaaggtga caaaatgggc ataccagtta
     1501 caatcgtttc tgggttttta ggggctggaa aaacaacgct aatcaatcaa gccttgcaag
     1561 tctcacccta ccccagggaa gaaattatta taattgaaaa tgagtttggt gaagttggga
     1621 ttgatcacaa attattagtt catagcgagg aacgggtgtt acaactaaat aatggatgta
     1681 tgtgttgcag tcttcgcagt gatttattag caatattgat ttctttactg gaaacaatag
     1741 aagaacatca acaaccaatt tcacaaatca tcattgaaac tacaggtata gcagatcccc
     1801 aaccaatcat tcaaacactt ttaacagcgc ctcaactaag aggaagagta tatatcgata
     1861 gtttattgac tgttattgat agtgacagtt ttgaacttat tgaaagagaa ccacaagccc
     1921 ttaagcaact tgtcatggca gatcgcattt ttgtgtctag aaaacaaa
//
LOCUS       DAZKCY010000231         1951 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000231 DAZKCY010000000
VERSION     DAZKCY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.240"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="ACG8XC_002837"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="ACG8XC_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFP9192470.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT          698 a          385 c          353 g          515 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa a
//
LOCUS       DAZKCY010000232         1901 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000232 DAZKCY010000000
VERSION     DAZKCY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.241"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            274..870
                     /locus_tag="ACG8XC_002838"
     CDS             274..870
                     /locus_tag="ACG8XC_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP9192471.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(928..1410)
                     /locus_tag="ACG8XC_002839"
     CDS             complement(928..1410)
                     /locus_tag="ACG8XC_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192472.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1516..1713)
                     /locus_tag="ACG8XC_002840"
     CDS             complement(1516..1713)
                     /locus_tag="ACG8XC_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192473.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          653 a          370 c          284 g          594 t
ORIGIN      
        1 tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat atatcagctg
       61 tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa ctaagaaaat
      121 ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat tttttgtgag
      181 catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc aattaaaaaa
      241 atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga cactttgaaa
      301 aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga agaattaaca
      361 actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca atcaacacgc
      421 gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga agaagcactt
      481 cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc tggtttcaaa
      541 aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg tctacaagaa
      601 caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa cggaatcgca
      661 acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt gcaacattac
      721 aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa ctggaaacta
      781 cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga atacatgaac
      841 gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac agaggttgtg
      901 acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat caatctctgc
      961 ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg cagaaaattt
     1021 cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt tagaaccttt
     1081 tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt tgtcaaagct
     1141 attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac taataaaaat
     1201 cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga tgagtgtata
     1261 ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat ttcgcacagt
     1321 ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa caacgaataa
     1381 aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt cttctccaga
     1441 aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag cacgccgcac
     1501 gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc ttgctttttc
     1561 atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg cgcagtttgt
     1621 catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt tgcatgcata
     1681 ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact gctggaactt
     1741 ttctccacat taccactgct atttctttgg agatttaact cattgatttt tatttacagt
     1801 taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat taaaacagct
     1861 gttgtattta gaagaatttt tttcaaaaaa ttaaaacata t
//
LOCUS       DAZKCY010000233         1884 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000233 DAZKCY010000000
VERSION     DAZKCY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.243"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG8XC_002841"
     CDS             <1..888
                     /locus_tag="ACG8XC_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFP9192474.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(932..1630)
                     /locus_tag="ACG8XC_002842"
                     /pseudo
     CDS             complement(932..1630)
                     /locus_tag="ACG8XC_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            1709..1884
                     /locus_tag="ACG8XC_002843"
                     /pseudo
     CDS             1709..1884
                     /locus_tag="ACG8XC_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          681 a          304 c          321 g          578 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc acaaagaggg ggggggattt tttatcgttg tggaatattt aaataccaac
      961 gcttgtcatg aaaatcttgt gctccaccct tagtatttcc ttctggatcg tttgtcgcac
     1021 gcatcatgac gtatactttc ttattaggga aatcacgcat attgaaagat acatgatagc
     1081 caacatttcc taaagtatta taagcttgat ttacgtctgg tctataaatc ccatcagctc
     1141 ttactctagc tagttctttt ccagtattgt agtccataat gaaaatatac tcgtatttat
     1201 aattagcaat gtgccatcca gctacatgca agtttgcgtt ttcgatttct ccgaactgat
     1261 caatgtgggc gtaatttgtt ccatctgtca gcgtagaatt tgcagcacct gatcgagttg
     1321 gatcaattac tggtttatcc tccgaagtag ttggattatc atccgtaaaa ccatgagcca
     1381 aatcataggc tagtttttct ttacttacac ccatttcaga aagataaccg taaggatctg
     1441 tatgatcgcc ccaaatattt tgtgttaccc ataaatgcga tttgattcct ggttggttat
     1501 aaggagtgtc taatgttaat ggaataccat atttcattgc tgaatctctt gccaattcaa
     1561 cgtatacttt atagttcttt tcaaaagttt ctttatcatg tgtgtgttgt aactcgattt
     1621 gcacaggact tttggctcac gaggttgtcc tactctacat cttgctgata gatatgctga
     1681 aaggaaaaca ccaacattat gttttgcttt ttcagctaga acgagacaag caggaatacc
     1741 tttagaagat atcaaagatt ttttaggtca taaagatgtt tccactacac aagtctatgc
     1801 ccatatttct cccgaagtga aaaaaagatc aatgaatcaa ctagaaaact atatagaaga
     1861 gcaaataaaa aagcactcaa attg
//
LOCUS       DAZKCY010000234         1865 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000234 DAZKCY010000000
VERSION     DAZKCY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.244"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..320
                     /locus_tag="ACG8XC_002844"
                     /pseudo
     CDS             1..320
                     /locus_tag="ACG8XC_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(592..882)
                     /locus_tag="ACG8XC_002845"
     CDS             complement(592..882)
                     /locus_tag="ACG8XC_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192475.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1090..1781)
                     /locus_tag="ACG8XC_002846"
                     /pseudo
     CDS             complement(1090..1781)
                     /locus_tag="ACG8XC_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          501 a          362 c          289 g          713 t
ORIGIN      
        1 atctacgata gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg
       61 acctatggat tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata
      121 ttgcttttaa aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag
      181 gtttaactgt cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt
      241 acgactatga taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat
      301 cctacaattc cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc
      361 cccattgagc gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc
      421 ctcaaatgag tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata
      481 tatagattga ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc
      541 catctttcca aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat
      601 tctttcatct tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat
      661 tttgggtttc cctaacacat gataggatct cacaacaaat tgctttctat tattataata
      721 acctgtaacg actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga
      781 agcaatcaaa caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg
      841 caaagtacgg atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc
      901 attatacgaa catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt
      961 tgtctattac ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact
     1021 aaaaaaaaga ggcagcagta tagattactg ttgcttcttt actaatttag gtttacttgt
     1081 taagaaagat tatttacgca aatgttcttc aacaatttgc tgagatttat cagctgtatc
     1141 ttccacttca tcttttgctt tttctaactt ttgaatatat tgtttcaagc ttgagttttc
     1201 agcagttgct tcgtctaatt tcttttgtgt atctgttaac tgatttctac atttgttttt
     1261 tcttgtgtca gtgttgcgat ttgttgattc aaaccattta cagtattttg taattctgaa
     1321 agttttgctt gtgcttcttg tagtcttgct gtccaatctg cattcagatg attgatttca
     1381 tcattttttt ggttgatctc atctagtttg acattgattt cattttgttt gctttgtagt
     1441 tgtgcatcct tgctgactaa ttgactttct aagttttgtt tttctgctgc cgattgattt
     1501 ttttggtttt ctaattcatt tacttttgtt tggtattctt ggacatctgc ctttgaatct
     1561 tgcaatgtct gttctttttg tgctagtcga gcggtaagat ctgtaatcgc ttgctcttta
     1621 tctgtcaatg tatgatcaag ctgtgtcaga ttctcattga ttgtatcaat atctgtctct
     1681 ccaccccaca aaataatttc atttgcgtac cgcgtgcctg tgtagccaat tagacataga
     1741 aatgcaacga caatcccacc agtaataaat ttctttttca tttgcatagc tccttaatcc
     1801 aatactttta catgctaaaa gtaagtgatc tcaaacacaa tgtcaagcgt ttttaaatta
     1861 catat
//
LOCUS       DAZKCY010000235         1852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000235 DAZKCY010000000
VERSION     DAZKCY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.245"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG8XC_002847"
     CDS             <1..1087
                     /locus_tag="ACG8XC_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9192476.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..>1852)
                     /locus_tag="ACG8XC_002848"
     CDS             complement(1305..>1852)
                     /locus_tag="ACG8XC_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9192477.1"
                     /translation="FYEEFYKRLMRFIFVTGVRSEELLALQWKDFDLINGRCSVNKSI
                     YIRTRQDYEFAETKNTSSIRIISLDRQTVEDLKTWKKTQQSIGKNIELVFSFDGLPPS
                     PRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFLIEHNKNIYAISKRLGHASIKTTLDK
                     YGHLYPETNQALANEITRFDI"
BASE COUNT          637 a          328 c          328 g          559 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa at
//
LOCUS       DAZKCY010000236         1789 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000236 DAZKCY010000000
VERSION     DAZKCY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.246"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1634
                     /locus_tag="ACG8XC_002849"
     CDS             156..1634
                     /locus_tag="ACG8XC_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9192478.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          662 a          243 c          312 g          572 t
ORIGIN      
        1 tccatttttt gcaatattct caaaaaaaag ataaaaaatg agaataactg atgaaaaaac
       61 aagcataatc catcataaaa tcagtatagg tgaaaagatg aactttcatc tgcatatcaa
      121 gtaattggta tgaggcattt tttgaggtgt aggatatgaa gttggattac cttatagaag
      181 aggaagatag agaaaaactg ctgctagcta gatatttaga atttgagaaa aagaattatg
      241 tagtgctgcc taagttatat gaaacattag gtttgtctaa atttaaaaca agaaattata
      301 tcatagaatt aaataaagaa tttactgaat tcaaaacaaa gccagagatc caaatttcag
      361 aagaagcaga gatccatgtc gaacgtctga atttgacgga tatcaaaaat ttaagaagaa
      421 tctactttca acgatcaaaa attgctcgac tgttaacgga gatcgtggat tcaaaagtaa
      481 cgatcgaaaa atttgcagag aatcattttt taagcatatc aagagcctat gtaaaaagaa
      541 gagaattagt ctcttttctt gaacagcatc aaataaagtt aaagaaaaac atattagttg
      601 gggaagaaat aaacatacgg aacgtattat ttggggtata tttcggggtc tttaatggtt
      661 tttctatgcc atttagagaa agcgtcatga atgaagcaaa gagtatcgtg caatattttg
      721 aatatctgtt caatttaaaa ctgtctgcaa caaaacgaat caaattcaat ttgctaacca
      781 ctattagttt gttgagggcc tatgggaatc aaccaatcgg aaaacgtttc ttttcagatg
      841 aatactttaa gagctcagaa ggaatgaaga tcatcaagga tttatcacat atttcttttc
      901 ttcatatccc taaagagcag atcattgatg agatttctta tatcatgttg tttttgatca
      961 cggaagaaga atttgaatta gatggcgcat ttggacttgc ggaagctcat tttcatgata
     1021 tccacatgat tagtcaaaaa atgtcagtgg aattgattga tcaattacca ttttcgattg
     1081 aaatctctga agaagaaaga ctatatatac gaaaagaact tcgtatgggt atgatgcatg
     1141 tcaatcgaaa aaattggata ttcgactttg agatgactag ctttacgtct aaaaagcagc
     1201 tacagttttt cttcgagacg tatccaatat tcagtacagc aatccaaaag gtcatacaag
     1261 agaataaacg ctatttcttg ggaaaagatg aacgtttcat gattcgctta ttttatgatt
     1321 atctcttttt attggtcggt atttgtccgg tatcatattt tgaagcaccg attcatgtct
     1381 gtattgattt ttcgcaagga tctgcttata ccgattacat tatttctcaa gtgaaaggat
     1441 ttaaaaatta caatattgtg attgagagtc gaatcacttc tcgtacgaat atttatttat
     1501 ctgattgttt acttgaaaaa tttaataaag aacagataat ttggaaaaat ccaccgactc
     1561 cagatgactg ggaatatttt ggaaatacca tcatcagaga aaaaaataga catttaactg
     1621 aaaaaatcag ctagtattta tggatggcag ctttgtctca catatttatt agctgattta
     1681 tgtttttttc aaagaaaagg taactagata taactagata taactagata tattacttaa
     1741 caaaattaaa aagtacataa gttattattc cgattttctt gaaacaata
//
LOCUS       DAZKCY010000237         1781 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000237 DAZKCY010000000
VERSION     DAZKCY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.247"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..326
                     /locus_tag="ACG8XC_002850"
     CDS             <1..326
                     /locus_tag="ACG8XC_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9192479.1"
                     /translation="KASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQR
                     EGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKL
                     SEVNN"
     gene            complement(548..1720)
                     /locus_tag="ACG8XC_002851"
     CDS             complement(548..1720)
                     /locus_tag="ACG8XC_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFP9192480.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          547 a          335 c          337 g          562 t
ORIGIN      
        1 aaaaggcaag tttaaaatca ctaaaagata catggcttga tttatcagaa gataatccat
       61 acagccaatt cttaattact gtaatggctg gtgttaacca attagagcga gatcttattc
      121 ggatgagaca acgtgaaggg attgaattgg ctaagaaaga aggaaagttt aaaggtcgat
      181 taaagaagta tcataaaaat cacgcaggaa tgaattatgc ggtaaagcta tataaagaag
      241 gaaatatgac tgtaaatcaa atttgtgaaa ttactaatgt atctagggct tcattataca
      301 ggaaattatc agaagtgaat aattagccat tctgtattcc gctaatgggc aatattttta
      361 aagaagaaaa ggaaactata aaatattaac agcctcctag cgatgccgaa aagccctttg
      421 ataaaaaaag aatcatcatc ttaagaaatt cttagtcatt tattatgtaa atgcttataa
      481 attcggccct ataatcagtc aagtccataa tcctgtgtaa aatactatac aatgttttac
      541 cggtctctca ttgatcaaac ttaatatatt ttcttgtaga actgagccat tcgtcatgaa
      601 ggtccataag gacagctcca attaatcgat tggcagacgt tcggttggga aaaatccgaa
      661 taatcttttc tcttctgcgg acttcctggt tcagtcgttc aagaaggttg gtgcttttta
      721 gccgattatg actatttccg ataaccgtgt attgaaaggc atcttcgaag ccattatcca
      781 atatttcgca ggcttttgta tatttaggct ggtcgatata ttcaccgact aaggcattct
      841 tagctgttcg agcgagttca atatccgtaa acttaaagat cgcttttact gcttctctaa
      901 acggttttga attctttttt ggaatggaac tgaagatgtt tcttaaaaaa tggacctggc
      961 atctctgcca acttgcgttg gtaaatgact tacgaatcgc agacactagg cctttatggg
     1021 catcagaaat gatgagttct gtccccttta ggccgcgttc ttttaagtat tcaaagaaga
     1081 tggaccatgt gtcatcactt tcttcatttt gaatcatgaa gccaatgatt tcacggtcgc
     1141 caccttctgt tatcccgatc gcaatatggc agcttttaga aagcactcga tggtcctcac
     1201 ggacttttat gtagagaaca tcagtcatca gataaggata attcgtacct gagagtgaac
     1261 ggttctgcca ttcgttgacc atcgggtcta actgctcagt caggctagaa acaaaagatt
     1321 tcgatacaga ttttccacat agctcttcaa caatctttga aactttacgt gtcgaaacgc
     1381 ccgaaacata catctcaagc attgaagcga gcagtgcctt ttcatttcgc tgataacgct
     1441 caaacaccgt cggtgaaaat tcaccatcac gtgttctagg cacttttaat tcgagtgtac
     1501 ccacacgagt cgtaaagtct cgctcataat agccatttct ttgactctga cgactttctg
     1561 aacgttcata gtcatcggct tgaatatatt ctgttcgttg attttccatc aattggttga
     1621 aaacagtggt taaaatattt ttagaaacat catcttttac cgaatgttca ataatacttt
     1681 gaacctcttc gttgttcagt gtaaaatgta cttgggtcat gtaacgtcct cctgggtatg
     1741 tttttgtggt taaaaacatt gtaccgtaaa agggctgtta c
//
LOCUS       DAZKCY010000238         1664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000238 DAZKCY010000000
VERSION     DAZKCY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.249"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8XC_002852"
     CDS             131..505
                     /locus_tag="ACG8XC_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9192481.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8XC_002853"
     CDS             complement(619..1248)
                     /locus_tag="ACG8XC_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9192482.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8XC_002854"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8XC_002854"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZKCY010000239         1650 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000239 DAZKCY010000000
VERSION     DAZKCY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.250"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG8XC_002855"
     CDS             <1..1412
                     /locus_tag="ACG8XC_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFP9192483.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
BASE COUNT          654 a          250 c          354 g          392 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa
//
LOCUS       DAZKCY010000240         1647 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000240 DAZKCY010000000
VERSION     DAZKCY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.251"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG8XC_002856"
     tRNA            complement(20..91)
                     /locus_tag="ACG8XC_002856"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG8XC_002857"
     tRNA            complement(113..200)
                     /locus_tag="ACG8XC_002857"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG8XC_002858"
     tRNA            complement(225..298)
                     /locus_tag="ACG8XC_002858"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG8XC_002859"
     tRNA            complement(312..382)
                     /locus_tag="ACG8XC_002859"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG8XC_002860"
     tRNA            complement(384..459)
                     /locus_tag="ACG8XC_002860"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACG8XC_002861"
     tRNA            complement(478..551)
                     /locus_tag="ACG8XC_002861"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACG8XC_002862"
     tRNA            complement(564..653)
                     /locus_tag="ACG8XC_002862"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACG8XC_002863"
     tRNA            complement(677..750)
                     /locus_tag="ACG8XC_002863"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACG8XC_002864"
     tRNA            complement(764..837)
                     /locus_tag="ACG8XC_002864"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACG8XC_002865"
     tRNA            complement(865..937)
                     /locus_tag="ACG8XC_002865"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACG8XC_002866"
     tRNA            complement(957..1030)
                     /locus_tag="ACG8XC_002866"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
     gene            complement(1040..1113)
                     /locus_tag="ACG8XC_002867"
     tRNA            complement(1040..1113)
                     /locus_tag="ACG8XC_002867"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:acg)
     gene            complement(1124..1209)
                     /locus_tag="ACG8XC_002868"
     tRNA            complement(1124..1209)
                     /locus_tag="ACG8XC_002868"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Leu,seq:taa)
     gene            complement(1226..1297)
                     /locus_tag="ACG8XC_002869"
     tRNA            complement(1226..1297)
                     /locus_tag="ACG8XC_002869"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Gly,seq:gcc)
     gene            complement(1309..1381)
                     /locus_tag="ACG8XC_002870"
     tRNA            complement(1309..1381)
                     /locus_tag="ACG8XC_002870"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:tgt)
     gene            complement(1399..1480)
                     /locus_tag="ACG8XC_002871"
     tRNA            complement(1399..1480)
                     /locus_tag="ACG8XC_002871"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1444..1446),aa:Leu,seq:tag)
     gene            complement(1488..1560)
                     /locus_tag="ACG8XC_002872"
     tRNA            complement(1488..1560)
                     /locus_tag="ACG8XC_002872"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Lys,seq:ttt)
     gene            complement(1564..1636)
                     /locus_tag="ACG8XC_002873"
     tRNA            complement(1564..1636)
                     /locus_tag="ACG8XC_002873"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Val,seq:tac)
BASE COUNT          438 a          460 c          391 g          358 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
     1081 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
     1141 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
     1201 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
     1261 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
     1321 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
     1381 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
     1441 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
     1501 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1561 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1621 gctgagctaa acctccatgg ataactt
//
LOCUS       DAZKCY010000241         1598 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000241 DAZKCY010000000
VERSION     DAZKCY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.252"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            145..1008
                     /locus_tag="ACG8XC_002874"
     CDS             145..1008
                     /locus_tag="ACG8XC_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFP9192484.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            1113..1586
                     /locus_tag="ACG8XC_002875"
     CDS             1113..1586
                     /locus_tag="ACG8XC_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9192485.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
BASE COUNT          570 a          252 c          325 g          451 t
ORIGIN      
        1 gaaaaagggg agtatgacac aactgttgtc atactccttt tttcaccttt ccaaaccaag
       61 gctcagctat tcaagtagat aacattatgt tatgatagca aagaaatgta ttaagaaaac
      121 ctttcactat agagggagaa aaaaatgaac gatttattag caactatatt tacaggtatt
      181 attattgatg aaaatgaaaa aaactacttt gtccaaaaaa acggacaaac ctttggctta
      241 agcaaatcag aaggtgaaca caaaatcggt gaagcagttg aaggattcgg ttaccaaaat
      301 caaaaacatg aaaatcggat cactacacaa attcctaaaa gccgtatcgg tcattacgct
      361 tttggaatcg ttactgctac acgaaaagat ttaggtgcgt ttgtcgatat cggtcttcca
      421 gataaagact ttgtcgtatc tcttgatgaa ttgccgacaa tgacagagct atggcctaaa
      481 aaaggggatc gcttgatgat ttcccttaga gttgatgcaa aagaccgtat ttggggcagt
      541 ttagcagatg aaaaaatatt caaatcttta agcaaatatg gaacagaaga aatgaaaaac
      601 aaagatgtga taggaacagc ataccgcttg aaattaacag gtacgtacgt tttaacggaa
      661 gatttctatc ttgggtttat ccatccttct gaacgatata atgaacctcg tctaggcgag
      721 cagataaaag gtcgagtgat tggtgtccga ccagacggtg tactgaatct ttcattgaag
      781 ccaagagcat atgaagctat atcagatgat gcacaaatga ttttaaccat tttagaacgt
      841 tctgcagatc agcaaatcaa ctttacagat aaatcggatc cagaagaaat catgaaagct
      901 tttggtatca gcaaaggagc atttaagcgc gcattaggta atcttttgaa acaaggattg
      961 attacacaag aaaacggtgt gacaaaacta gtcaaaaaat ctaattgaga atcccttgca
     1021 tgtttttctt aactagctta taataatttt aattaacata gtgttatatc tttttttatt
     1081 tgtaattatt ataaattgtt ggggttgagt aaatggtcat gaatgctatt tcggcgatga
     1141 aaaaaacaaa acagcagcta catgaagcgg ggtttaagct gacaccgcaa agagaagcta
     1201 ctctgcttgt gcttttggaa aatgagaaag agcatttatc tgcagaagaa atcttctttt
     1261 tagtgaaaaa aaagagtccg gaaattggac ttgctactgt ttatcgaacc cttgaaatct
     1321 tgacagaatt gaaaattgtc gataaagtaa gttttaatga tggattagcc cgttatgatt
     1381 tgagaaaaga aggcgccaaa catttccatc atcatttact atgcttgaac tgcggcacga
     1441 tcgaggaagt agaagaggat cttttacttg aagtggagca agtagtagaa gaacgttatc
     1501 attttttagt aaaagatcat cggctaacgt tccatggaat ctgtcaggaa tgctatgaaa
     1561 aagaaaaaag aaaccagtcc gtctgagtgt ctagacgg
//
LOCUS       DAZKCY010000242         1567 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000242 DAZKCY010000000
VERSION     DAZKCY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.253"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..381)
                     /locus_tag="ACG8XC_002876"
     CDS             complement(4..381)
                     /locus_tag="ACG8XC_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192486.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(681..1562)
                     /gene="galU"
                     /locus_tag="ACG8XC_002877"
     CDS             complement(681..1562)
                     /gene="galU"
                     /locus_tag="ACG8XC_002877"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9192487.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTGALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          430 a          354 c          240 g          543 t
ORIGIN      
        1 atattaccgt cttcttctcg ttcttctact ttgttcgaga gaatctgtac tagaaaaagt
       61 agttgtatgt ctaccttttc tgcctatccg agactttctt ttcatatatg ggatataaat
      121 gattagatac agtacagcaa aaaataaagc aaatacgcaa gcttcccaac tacctaacgc
      181 caattcaacc atcaaggtag caaccccaac ggatgctgca taccagataa aaatcagaaa
      241 cgtttctttt tttctgacca tgcgaaacat gataaataga ctaataagca atagtggcaa
      301 cacaaatatc caattgcttc cttccatcag ccacttgagt atatcaagca gcgactgtgc
      361 aacagcgact aattttccca tagtgaacac acaccttttt ttataattaa aaaattcgcc
      421 tttttattct actatgaatc ttctggcttg gctttcattt ctcttatgta atcattttct
      481 tttatacaac ttaaaaaaaa attaaaccat tagcagaata ttttctgaat ctattcacga
      541 aagtcttttt ctatctttcg ttttccacga ctcaactgat ctagagtcaa aaaactaaaa
      601 cgacagttgc gcaaatgacg cacaagctgc cgctttaata acaaatagcc tttcattctc
      661 agcaggccac ttatttccag ttaactttct gttcttagct ttttactcaa ctggataata
      721 tatgcactta atgaagtccc aatttccgaa tgttgaagtc catattcaat atttgtcttc
      781 atatatccta acttatcgcc tacgtcgtat ctttttcctt taaactctct cgcatagacc
      841 gcctctgtct ggttaagagt ctgaagtgca cccgttagct gtatctcgtt tcctgctcct
      901 ggttcttgag tttctaatat ctcaaatatt ttaggagtca gtagataacg tccgataatg
      961 gctaaattac ttggtgcttt tcccatttca ggtttttcta caaacccttt tactctgcaa
     1021 cgaccctttt cgctatctgt tttaggatca ataattccgt atttagatgt ttcattttct
     1081 ggtacacgca tgacggcaac agcggaagct tgtgtttctt cataatccat catcaattgt
     1141 tgagtcaatg ggatatcatc tttcatcaaa tcatccccca gcatgacgac aaacggttca
     1201 tttcctataa atgcttttgc ttgaagcacg gcatccccca gtcctcttgg atgagattga
     1261 cggatgaagt gaagattgac ttgagtcgtt tcttcaacta aagcgagtaa ttctgttttt
     1321 tccttttccc gcaaattgtt ttccaattcg atatttgcat caaagtgatc ttcaatgggt
     1381 cttttttctt ttcccgttac gatcaagatg tcttcgattc ccgaatccaa tgcttcttcc
     1441 acaataaact ggatcgttgg tttatccacg attggaagca tttcttttgc catggctttt
     1501 gttgccggca aaaaacgtgt tcctagccca gcggcaggaa tgactgcttt tttgaccttc
     1561 acttgct
//
LOCUS       DAZKCY010000243         1567 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000243 DAZKCY010000000
VERSION     DAZKCY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.254"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..1517
                     /locus_tag="ACG8XC_002878"
     CDS             51..1517
                     /locus_tag="ACG8XC_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFP9192488.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTKKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          435 a          289 c          330 g          513 t
ORIGIN      
        1 gtaaagatgt ataatatttt ctgaaaattt aaagaattgg ggtatagatt atggaaaaac
       61 agcaagacaa gggcttcttc gggcagccga agggattgtc aactttgttc ttcactgaga
      121 tgtgggaacg atttagttat tacggtatgc gtgcattact tatctattat atgtacgata
      181 cagttgccaa cgggggactt ggattaccac gagcaacagc attagctatc atgtcgatct
      241 acggatcttt gatctacatg tctagtatcg tcggcggatg ggtatctgac cgagtgttag
      301 gagcaaaacg cactgtattc ttcggtggag ttttcattat gcttggccac atcgtcttgg
      361 ctttaccatt tggtgtaagt acattgtttg tttcgatggc attgattatt atcggaaccg
      421 cctttttgaa accaaatgtt tctggcatgg tcggacatct ttattcaaag actgacttgc
      481 gaagagatgc aggattttcg attttttaca tgggtgtcaa cttaggggca ttattagcac
      541 cactaatcgt tggtacaatc ggtcaagaat ataactatca tttagggttc tctttagctg
      601 ctattgggat gttctttgga ttattacaat acatcatcca aggacgcaag acattagacg
      661 gagtgggtac aacaccgccg aatcctttga caactgaaga acgtaaagga ttcatccgta
      721 acttgatcat cgcacttgtt gttatcgctg cagtattcgg cggggcacaa ctaactggtc
      781 atctgacaat tgactttttt gtcaatacaa tcagtgtatt ggggattatc ttaccgttgt
      841 attactttat caaaatgctg acttcaaaag atgtaactgc tgaagaaaaa ccaagagtat
      901 gggcttatat cccattattc cttgcagcaa tcattttctg gtcattacaa gaacaaggtt
      961 catctatctt agctttattt gctagtgaac gtacacaatc tacgctattt ggtgtaacga
     1021 tccaagcaag ctggtatcaa tcattgaatc caattttcat ctttattctg acaccagtat
     1081 tcgtaacgat gtggacgaaa cttggtgaac gtcaaccatc tacagtagtg aaattctctc
     1141 ttggactttt atttgcagga ttatcgtact tgctattgat gttgccgggt atgctttatg
     1201 gtacaagtgg aaaagtaagt ccattgtggt tgttgggaag tttcttcatc gtggttattg
     1261 ctgaattgtg tctttcacca gttggtttgt ctgtgacaaa aaaattagca ccacgagcat
     1321 ttgaatcaca aacaatcgct atttggttct tagctgatgc ttcttcacaa gcaatcaatg
     1381 cacaaattgc acgcttctac acaccgggaa cagaatctgc ttacttcggt attatcggtg
     1441 cagtagcaat cgtcggtggt atcttgctat ttactgtgaa agaaccaatc aaaaaactaa
     1501 tgggctctat tcattaatca atgaacataa aaacaggaat tgcttatttg cagcagttcc
     1561 tgttttt
//
LOCUS       DAZKCY010000244         1537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000244 DAZKCY010000000
VERSION     DAZKCY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.255"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(855..1385)
                     /locus_tag="ACG8XC_002879"
     CDS             complement(855..1385)
                     /locus_tag="ACG8XC_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFP9192489.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1382..>1537)
                     /locus_tag="ACG8XC_002880"
     CDS             complement(1382..>1537)
                     /locus_tag="ACG8XC_002880"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9192490.1"
                     /translation="AFYFSAIIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILI
                     TKRDGER"
BASE COUNT          480 a          308 c          175 g          574 t
ORIGIN      
        1 atttaaaatt aacattgatt ctcctatgaa aatatctttt acctttctcc tgtgaaaagg
       61 aaatttcctt ttttatagtg ttattgatat aatcagaata ttcatcaaac tctgtatctg
      121 gagttaaaaa tctacagtaa catctataag ctttgctagt aatcttcttg ggttctctct
      181 ccattgtcaa gcaacatcaa aacacctact tactattcga ttcttttctg ccaaaaacga
      241 gtcagcatca ctcacaactt atgtgttgct gactccgact agtcctttgt tcaatatcaa
      301 cttattaaac agatataata tttttcttta tttcgtatac tccaagactt ccatctttct
      361 ttcatcttat ctctgaaaaa aacacagcaa agtgccctta ataaatatac attagaattt
      421 gcaaattttt tcatcttaag atatatccta ttcatattct ttacaatcct aatgggtgat
      481 tacaaatcta tttagttcaa gttctacatt ataaacacct ttcttgatag tatcatctgc
      541 tgcacaagcg atgtcgatcc acttattagg ggatttacgt gctcaaaagt ggctattcct
      601 tttgggaagt ccatccataa taatggtcca tggatttttt tagggtcttg tcgttcaaat
      661 ttctcaaaaa ctttattata cttccatagt tttataatta tttggtttca ctttggctcg
      721 ttcaaacacc aacttgtatt tcttttcaac gttcgaactg gagccccccc tataatctca
      781 ttattatttt catgactttt tactaccgtg gaattacaag caacaacaat attatctgct
      841 aaatgaacac ctttctatat cacgcttttt gctccaatcc aacaatgttt acctataact
      901 attttattat ttttattcaa aatcctatga gtattcaatt caaaaatcgt atgtgaatcg
      961 gtatccataa ataatgtatc ccacgatact gtacagccat ctttgaattg aatttcctta
     1021 aagcaaatta tactcatttt tcctgtcgaa ttaaactttg acccaatatc taaaactgca
     1081 tttttcctta tgtcaatatg agccccttgt gcgataaacg ctggcccttg tattcttagt
     1141 attccttcaa tacttataat cgtttcttca tttcccgcag tatttaatac tggaaagccc
     1201 aaataaacta tttttccaga ttgtaatact atctcaattt tccctctcga tttgcgtgta
     1261 atccgtactt tacgatgcac caaaataggt aatctccacg catctgaaat atgaagaaat
     1321 ttataattga agtatatcgt tttaggtaaa tttaatatat tctttattat caatccgagt
     1381 ctcatctttc accatctctt tttgtaatca aaattagttt ataaaggatt acacccaata
     1441 taccgcccaa cgtattatat acaaaatccg aaatttgaaa cgttcctagc ctcaaaaaaa
     1501 gttgtaaaaa ttcaattatt gctgaaaaat aaaatgc
//
LOCUS       DAZKCY010000245         1516 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000245 DAZKCY010000000
VERSION     DAZKCY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.256"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            781..1167
                     /locus_tag="ACG8XC_002881"
     CDS             781..1167
                     /locus_tag="ACG8XC_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9192491.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1261..1452
                     /locus_tag="ACG8XC_002882"
     CDS             1261..1452
                     /locus_tag="ACG8XC_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9192492.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          565 a          233 c          212 g          506 t
ORIGIN      
        1 aatactgaat tcggaattaa aaaccacgat aggtaacttt aaacaaacat gaagagactt
       61 taataagact tctttatctg aaaacacttt tgtttctctc tttcctttct acatcattaa
      121 atagtatttt atacgttatc taaaatacta ttcctctgtc aaaataagct aacttacttc
      181 attataaaat atccacttat cgctttcatg tatattccac cgtaaaaagt acattttatc
      241 accgagaact catcattttt catcatgatg aatactattt acatatatct agttcaaaga
      301 caataattat tatgatccgt tattatctaa gcactataca gataatagga atttttcgtt
      361 tctaaatatt ccaatttggg aatccaataa aaattttcgt tcaaatacgt gtctattcga
      421 actcttttct agttgttaat tatgaagggg gaagaaataa aaaatacaat ctatacacga
      481 ataagaatat tatccaaaag gaggttttag aaaagattta ttatacgtaa aaagctcaag
      541 acattttctt agccataagg acatgtttga tatttaagaa gatatatgaa ttttcttttc
      601 taagtataaa gataactatc cattatcaac taaaaaaaca tttttgaaaa taaacatcag
      661 cagtcctatc aatgcatgtg agcttataga agttattcgc agcgcagcat aattgcatta
      721 gcttcttttc gtgttttttg cctcttttcc ctttaaatct taatggagga ggtgtgcaat
      781 atgtcagata acgacgaacg cgtaaatgcc cagttgattc gattctttga aaaggtcatt
      841 ttgaacaccg ctaatactta ttataaaaaa caagaagaaa ttcaaaaaca tgaacaactt
      901 gatcaactcc caccatattt gtttactaaa gatactataa aatttaaaca accgattaat
      961 gttttaaatg tgactctcct tgttgaagat ataaaattag caaaaatcct tcctttgttg
     1021 aaagaacctg aacaaatttt tcttatcgaa aaatttatct ttgataaaac tgataaagaa
     1081 attggaaagg ttctggggat tacaagacag ggtgcgacaa atttaaaaca tcgactttac
     1141 aaaaaaattc aaaaaatgct ggtttaatat ctgcataata actaattttg tatgcttctt
     1201 tgctcgttct cctccttccc aaagaaatag attataagtc catgggaaga aggtagattt
     1261 atgaattctt taaaagaaga tttcatttta gcaaaggctg gaaatgaaga agctgttgag
     1321 gcaattctca aaagatttag ctctttgatt cataagcaat cctggagaac tggaaaatat
     1381 gatcaagatt gttatcaaga atgtatgctt gcaatatatc tagctatctc gaaattcgaa
     1441 attaaagaat aactcaacta agggagtctg tgacataagt aaaaagatag tttcaaattt
     1501 cgaacaataa atttga
//
LOCUS       DAZKCY010000246         1506 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000246 DAZKCY010000000
VERSION     DAZKCY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.257"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            236..826
                     /locus_tag="ACG8XC_002883"
     CDS             236..826
                     /locus_tag="ACG8XC_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFP9192493.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            853..>1506
                     /locus_tag="ACG8XC_002884"
     CDS             853..>1506
                     /locus_tag="ACG8XC_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9192494.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     "
BASE COUNT          527 a          169 c          295 g          515 t
ORIGIN      
        1 aaaaattcgt taggtttaaa taactggaaa tatcggaaaa tgggcattat acatcacgga
       61 catcccttct tttggaaaaa atgttacgat ttttataagt aatattgtat ccggtggaat
      121 caacgatgaa gataacatat ccatcgttgt agattcagta gcattgaatg atgctcttgt
      181 tgcaagaaat ccgaataaaa ttgttaagta taaattgaat taataggaga tatgaatgga
      241 aattagcagt aaagaatatt taaaagcggt tcttcgtatg gaaaagaatc tatatataga
      301 atctaaagga caacacacaa aattactaaa aataatgaaa tcacatccgg actacaacgc
      361 ttggcggtat actcgtcgaa tgagaattac atcttattat tattcaaaga gaaagaaaaa
      421 tatcatttat gcgattatgt atattgtgtc ttgcagaaga atgaatagtt tgggaagaaa
      481 atgtggtata gagtgtggtg aaaatgtatt tgatgaaggt gtaagatttt accatacaca
      541 aggaattgtt attaatggta acgccataat aggaaaaaat tgttacttat atgggaataa
      601 ctgtattgga aatgatggaa tcgatcctaa gtgtcctgta attggaaata atgtacgtgt
      661 atgcgtgggt gcaaagatta ttggtaatgt aaaaatagct aacaatgtag ttgttgcggc
      721 aggagctatt gttatcaaag attgtctaga gaatggtgca attcttgcag gtgtaccggc
      781 aaaaataata gggtatgtta atttaacaaa agtattttta gaataatttt tagcgaaaga
      841 aggtgaataa cgatgcgtat ttcaataaaa agatggtcag gagcagattg gacattgttc
      901 acgttggcat tttttgcaat ctccatagtt cttgtatata cttttaacgc cccaagagca
      961 attttatatt tgggagatat aattaatatc tttgtgttat taaatgcact tagctttaga
     1021 cgcagaaaag gaatatatgc tagcaaaaat gagatgctga tagttttgtt tttactagtt
     1081 tctatttgta gctcagttat taatttagaa aatataggat tgattatctg ggggatacga
     1141 aataacactc gtatgttcat agtatatata aattgtgcaa catatttaac aattaaagat
     1201 tactataaag tattcgctat aattgaaaaa ttattctgga tttcactgcc actttgttta
     1261 attgaaagat tcatggtcac ctatccaact ggaacaattg taggagatat gataggtgga
     1321 gttttttgga attattcagg ttgtaatatg ccattgaatt taattttgtg tgtgacagtt
     1381 actgttgtta caatcggata tttcaaggga gaaaaaaatt tcatccattt tattttatgt
     1441 gtttgtgcag cactttttat gtctgcatta gctgaattga aagtttttat tcctgaatta
     1501 tttata
//
LOCUS       DAZKCY010000247         1485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000247 DAZKCY010000000
VERSION     DAZKCY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.258"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            250..849
                     /locus_tag="ACG8XC_002885"
     CDS             250..849
                     /locus_tag="ACG8XC_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9192495.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            913..>1485
                     /locus_tag="ACG8XC_002886"
     CDS             913..>1485
                     /locus_tag="ACG8XC_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiH family protein"
                     /protein_id="HFP9192496.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT          521 a          231 c          296 g          437 t
ORIGIN      
        1 aacttaaaag tactgacaaa attttttaaa ggggcaatat taatgttctg agataatcaa
       61 atcctaccga aaatatcaaa aatccaatgt gacacaatca gattgtgtct cattcgaggg
      121 aatcacacaa tcccacgtgg ctctaggcag aattgaattt ttgcttgggg ctttttcctt
      181 atattgattt tgaatgagac ttattatatg ttataatgca attaagtatc attggagagg
      241 gcgaagaaaa tgaagacggt agaacctatt cgagacaaaa aaaagatcga tgcgatgaag
      301 gcaatcttgg cttcgggaaa atatggccaa cgaaatttgg ttcttttttc gattgggatc
      361 aacaccgcct atcgcatttc tgatttgcgt caattgaagc tggccgacgt gctggaaatt
      421 tcacgcggtc gcgtggtggt caaagatcga ttagcgatga aggaacaaaa aacagctaaa
      481 cataattcgg tctatatttc caacaaattg cgaaaaacga ttttggagta cgttcaacag
      541 gaatttcctg atcaactcct tcagcaagat tttgacggtt atttgttccc tagtcgaaaa
      601 gggacgaatg aaccgctcaa ccgccaaagt ctgtggcgaa tcattcatga tgctggcacg
      661 gcaatcggat tgaaagacat tggcccacat tccatgcgaa aaacgttcgg ttactttctc
      721 tataaacagg ggaccaaaac agaaatcatt cagtccttgt tgaaccattc ttctcagcgc
      781 gaaacattgc ggtatattgg tattacacaa gaagacaaag atacagctgt aaaaagctta
      841 gatttataat ataaaagtta agtttaaaca tggatagttt taaattagta gattgagtgt
      901 tgggaggaaa gaatgagtac attatatatt ataggaaacg gttttgattt aagtcatggt
      961 ttaaaaacta gatacactga tttttcaaaa tgggttgaaa aaagtcatag tgaaatattc
     1021 gataaaatag acacaatgat aaatgaaaat aatgaggtga gaagtacagc tgatgataag
     1081 ataggttggt ttaattttga aagtagtttg catgagttaa ttcaaagtcc agtctttgaa
     1141 gattatgtag aagagtctaa ggattatctc ataggttatg gacatgaaga ttggtcagat
     1201 tctgcccatc atgattttca aaatatggtt tatgattatg ttgtttctct agaagaatta
     1261 caacaacttt tcaaggaatg gatacgcaaa gaaatacagc cgacaataga acataaaatt
     1321 ccaaaatacc aattttcgtc cgatgtacta ttctttacat tcaattatac agatgttttg
     1381 gaaattctat acaagcaacc gaaagaaaac atatatcata ttcatggcag ttatgacgat
     1441 ataataatcg gccactccga gaattttgac aactttatag gagat
//
LOCUS       DAZKCY010000248         1470 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000248 DAZKCY010000000
VERSION     DAZKCY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.259"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..>1470
                     /locus_tag="ACG8XC_002887"
     CDS             234..>1470
                     /locus_tag="ACG8XC_002887"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP9192497.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLL"
BASE COUNT          481 a          233 c          276 g          480 t
ORIGIN      
        1 tgaatattgt aacttgtgaa taaggaatta gcaggagaat actagttcag cataaaggaa
       61 gtgatgggac ttctgagaca actttctgtt atgcagaaat acacatataa acagacaata
      121 aacgcttttc cccgtttctt ttaatttcct ttacaatttg gttattcact aggtttttaa
      181 actttgctat ggtaagatga tagaggcatt attttatggt taggagtgta tatatgaata
      241 aaaatgggga gtggaatgcg gcacgcagaa tattcattat tcttatggat gtacttgtat
      301 taaatgtatc gatctatctt agttttttgc tgaaattcca gggagaagta ccagtgagaa
      361 acttagaaac ctttcagcat tcagcaattt ttatttccat catctttatt gcgttgaata
      421 ttttgttagg agcttatgta ttctataacc gaatgatcag tgatattgtc ttcattactg
      481 ttattggaca agtactaata gcattgggga tcatggtagt tacttttgct ggccgatggt
      541 ttgcatttcc gcgtggcgtc attttgatct cgtttatcct aggtacgata ttacttagtg
      601 tttaccggat agtcgtttac aaactgtact taagagtcag tcgtgataag aaagtcctta
      661 tcgtgggttt ggagaaagat gtcgcaccag ctatccaaaa ttttcaatct aagaaaaaca
      721 ataaacataa ggttgaagca gtggcaatcg ataattatta cgaaaataca aaaaaattga
      781 tcgatgatat ggatatcgtc tatttggctt ctaaaatccc agaagatgaa aaaatcaaca
      841 tttatcaatt agtgactaaa aaagagaaga aattattttt aaatacgact tttgagaatc
      901 tgacaatgat aaatccgaat attatgaatt ttgaagatga aagcatcatt gaagcttctg
      961 ggttcagaat ccaaccagaa tacgaactat tcaagcgtct ctttgatatc atcgtttcgc
     1021 ttattttact gatcctcgcc tcacctttta tgctaatcac agcgattttg gtaaaaacaa
     1081 cttcacctgg accaatcatc tataaacaag tacgtattac gaaaaatcaa aaggaatttt
     1141 caatctataa attccgttcc atgagtgcga cagctgaagc aaaatcagga ccagtcttgg
     1201 ctaaaagtaa cgatgcacgt gtgacaccag taggtaaatt tatccgtgct gtacgttttg
     1261 acgaattacc tcaaatcttt aatgtattga aaggcgatat gtcgattgtc ggtccaaggc
     1321 cagaaagacc atttttcgtt gatcagttca atgaagaaaa tccttattat tacttaagac
     1381 acaatgtacg tgcgggaatt accggatatg cacaagtata cggaaaatat gtatcagatt
     1441 ataacagtaa actacgtttt gatttgttgt
//
LOCUS       DAZKCY010000249         1457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000249 DAZKCY010000000
VERSION     DAZKCY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.260"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(23..107)
                     /locus_tag="ACG8XC_002888"
     tRNA            complement(23..107)
                     /locus_tag="ACG8XC_002888"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71..73),aa:Leu,seq:caa)
     gene            complement(125..196)
                     /locus_tag="ACG8XC_002889"
     tRNA            complement(125..196)
                     /locus_tag="ACG8XC_002889"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(162..164),aa:Gln,seq:ttg)
     gene            complement(206..278)
                     /locus_tag="ACG8XC_002890"
     tRNA            complement(206..278)
                     /locus_tag="ACG8XC_002890"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:His,seq:gtg)
     gene            complement(340..424)
                     /locus_tag="ACG8XC_002891"
     tRNA            complement(340..424)
                     /locus_tag="ACG8XC_002891"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(388..390),aa:Leu,seq:caa)
     gene            complement(445..515)
                     /locus_tag="ACG8XC_002892"
     tRNA            complement(445..515)
                     /locus_tag="ACG8XC_002892"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(481..483),aa:Cys,seq:gca)
     gene            complement(561..632)
                     /locus_tag="ACG8XC_002893"
     tRNA            complement(561..632)
                     /locus_tag="ACG8XC_002893"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(598..600),aa:Gln,seq:ttg)
     gene            complement(644..716)
                     /locus_tag="ACG8XC_002894"
     tRNA            complement(644..716)
                     /locus_tag="ACG8XC_002894"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(681..683),aa:His,seq:gtg)
     gene            complement(719..792)
                     /locus_tag="ACG8XC_002895"
     tRNA            complement(719..792)
                     /locus_tag="ACG8XC_002895"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(758..760),aa:Trp,seq:cca)
     gene            complement(799..879)
                     /locus_tag="ACG8XC_002896"
     tRNA            complement(799..879)
                     /locus_tag="ACG8XC_002896"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(843..845),aa:Tyr,seq:gta)
     gene            complement(881..956)
                     /locus_tag="ACG8XC_002897"
     tRNA            complement(881..956)
                     /locus_tag="ACG8XC_002897"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(921..923),aa:Phe,seq:gaa)
     gene            complement(992..1064)
                     /locus_tag="ACG8XC_002898"
     tRNA            complement(992..1064)
                     /locus_tag="ACG8XC_002898"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Asp,seq:gtc)
     gene            complement(1074..1146)
                     /locus_tag="ACG8XC_002899"
     tRNA            complement(1074..1146)
                     /locus_tag="ACG8XC_002899"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1111..1113),aa:Val,seq:tac)
     gene            complement(1162..1233)
                     /locus_tag="ACG8XC_002900"
     tRNA            complement(1162..1233)
                     /locus_tag="ACG8XC_002900"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1198..1200),aa:Glu,seq:ttc)
     gene            complement(1248..1337)
                     /locus_tag="ACG8XC_002901"
     tRNA            complement(1248..1337)
                     /locus_tag="ACG8XC_002901"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1301..1303),aa:Ser,seq:gga)
     gene            complement(1377..1450)
                     /locus_tag="ACG8XC_002902"
     tRNA            complement(1377..1450)
                     /locus_tag="ACG8XC_002902"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1414..1416),aa:Asn,seq:gtt)
BASE COUNT          413 a          383 c          335 g          326 t
ORIGIN      
        1 aaagttgcta tattgataat tataccggcg gccggggtcg aagcggcacg tcctcaacgg
       61 acactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaaa tttaaaaata
      121 ataactgggg tagctggatt cgaaccaacg catgagggag tcaaagtccc ttgccttacc
      181 gcttggctat accccaagaa taaagggcga atgatgggaa tcgaacccac gaatgccgga
      241 gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tcataaaaga tgttatttaa
      301 tttctcaata aaaaactaag atgaagacat cttagtcaat accggcggcc ggggtcgaac
      361 cggcacgccc tcaacgggca ctggattttg agtccagcgc gtctgccaat tccgccacgc
      421 cggcaagttg atttattagt actaaggcgg taaccggatt tgaaccggtg atgaaggttt
      481 tgcagacctc tgccttacca cttggctata ccgccattat actattatca atctataaaa
      541 taaagaataa attatagaaa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa
      601 agtcccttgc cttaccgctt ggctataccc caagaataaa aagggcgaat gatgggaatc
      661 gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac attcgccatg
      721 gcaggggcag caggaattga acccacacta acggttttgg agaccgctgt tctaccttta
      781 aactatgccc ctataaaatg gaggagagtg gattcgaacc actgaaccct aaggaacgga
      841 tttacagtcc gtcgcgttta gccacttcgc tactcctcca tggtggcgcg ggacagaatc
      901 gaactgccga cacacggagc ttcaatccgt tgctctacca actgagctac cgcgccatat
      961 tttaaaatta ttaattgaat aacaagaacg acggttccga cgggaatcga acccgcgatc
     1021 tcctgcgtga caggcaggca tgttaacccc tacaccacgg aaccagttca ttatggaggt
     1081 taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca gctgagctaa
     1141 acctccaaca ttcaattaaa atgacccgta cgggattcga acccgtgtta ccgccgtgaa
     1201 agggcggtgt cttaaccgct tgaccaacgg gccaggtaaa tttgctacgg agagtaaggg
     1261 attcgaaccc ttgagacagt ttggcaccat ctacatgatt tccaatcatg ctccttcggc
     1321 ctctcggaca actctccaga gtgaaagaag ctcaatactc tttctcaaaa tgagaactcc
     1381 ggcagtagga ctcgaaccta cgacatcatg attaacagtc atgcgctact accaactgag
     1441 ctatgccgga ataacat
//
LOCUS       DAZKCY010000250         1451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000250 DAZKCY010000000
VERSION     DAZKCY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.261"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            468..>1451
                     /locus_tag="ACG8XC_002903"
     CDS             468..>1451
                     /locus_tag="ACG8XC_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFP9192498.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQG"
BASE COUNT          510 a          274 c          256 g          411 t
ORIGIN      
        1 aaaaagttca aaagatcaag tgtgcgcttc gctcccactc ccctcgacgt tttagtagcc
       61 tttccctcac ttcgttcagt ccaagccaac taaaaagttt tcgggataca ttctccttct
      121 cttcctaata atcaattaaa attttaatct ttatatttct gctaatcact caggaaacag
      181 tacagaaaaa caaacacgta tcatagatat aaatgtaatg gcatagtgcg ggttttattt
      241 tcagcctgta tcatagctaa acaaatcgag ttgtgtgtcc gtttttgggc gttctgctag
      301 cttgtttaaa gtctcttgaa tgaatgtatg ttctaagtca aaaaactctg acagcgcctt
      361 tatatagata aagtccgtat aattgtgtaa aagtaaaaag gccatataac agtcctttta
      421 cggtacaatg tttttaacga caaaaacata cccaggagga cttttacatg acccaagtac
      481 attttacact gaaaagcgaa gagattcaaa gcattattga atattctgta aaggatgacg
      541 tttctaaaaa tattttaaca acggtattta atcaactaat ggaaaatcaa cgaacagaat
      601 atattcaagc aaaagaatat gaacgaacag aaaaccgaca aagtcaacga aatggctatt
      661 atgagcgcag ctttacgaca cgtgtaggca cgctagaatt aaaagtaccc agaacacgtg
      721 atggccattt ttcacccaca gtgtttgaac gttatcaacg aaacgaaaaa gccctcatgg
      781 cttcaatgtt ggaaatgtat gtatcaggcg tttcaactcg taaagtatca aaaattgtgg
      841 aagaactttg tggtaaatcc gtctctaagt ccttcgtttc tagcttaaca gaacagctag
      901 aacctatggt taacgagtgg cagaatcgtt tattatcaga aaaaaattat ccttacttaa
      961 tgaccgatgt actctatata aaagtacgag aagaaaatcg agtactctca aaaagctgtc
     1021 atatagcgat tggaataacc aaagatggcg accgtgaaat tatcggcttc atgattcaaa
     1081 gtggcgaaag cgaagagacc tggacaacat tttttgaata cctaaaagaa cgcggtttac
     1141 aaggtacgga actcgttatt tctgatgcgc acaaaggatt agtctctgcc attagaaaat
     1201 ccttcaccaa cgtaagttgg caaagatgcc aagttcactt cctaagaaat atctttacca
     1261 ccattcctaa aaaaaattca aaatctttca gagaagctgt taaaggaatt tttaagttca
     1321 cagatattaa cttagcgcgt gaggctaaaa atcgattgat tcatgattat atcgatcaac
     1381 caaaatattc aaaagcttgc gcatcattgg atgatggatt cgaagacgcc tttcaatata
     1441 ccgtacaagg a
//
LOCUS       DAZKCY010000251         1450 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000251 DAZKCY010000000
VERSION     DAZKCY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.262"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..873
                     /locus_tag="ACG8XC_002904"
     CDS             202..873
                     /locus_tag="ACG8XC_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9192499.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(1033..1224)
                     /locus_tag="ACG8XC_002905"
                     /pseudo
     CDS             complement(1033..1224)
                     /locus_tag="ACG8XC_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          520 a          216 c          194 g          520 t
ORIGIN      
        1 taccatatca gaatgattgt attttttatt cagaaaaata aaatactcct tctttttgga
       61 ctgattttat cgaaacttat tttttctttt tttcatgtat tcttttattt ttataactag
      121 tgtattactt caaaatttga tattatatgg catgaacaga attaaaatat cagttaaaat
      181 aaaagttaag gagcattgtg tatgacaatt aaaactagtc tccaaaatca agcttaccaa
      241 tctattcgcc gacaaattat ctatgctgac ttagaacctg gaaaaaaagt atcagaaaaa
      301 aaattagaag aaacactttc tattggtcgt acaccaatac gtgaagcact gattcaactc
      361 cgacaacaag gtcttgttga tacaattcct caaagcggaa attatatctc attaattgat
      421 ttagattcgg ccaaaaatgc tcgatttgtc agagaacatt tggaacgaca aattatgtta
      481 gagtgttgtg ccaaaatgaa taaccaaaaa aaacggattt tagaaactat aatttcagaa
      541 caagaaaaag ctgttagtca aaaaaatgct cgtagttttt ttcataatga taatttattc
      601 cataaagctt gttttgaaat tgcagagcga gaagaagtat ggcaatggtt agaaaataac
      661 aatacacact tagaacgatt tcgatggcta cgagtaaaaa cggaaggatt aaagtgggaa
      721 aaaattatgg aacaacatta tcaactattt gaggcattaa caaaacaaga tccagaagaa
      781 gctaactttc taacatcgtt gcatcttcat ttaatgctta atgagcaaaa tattgtttta
      841 gctactcatc caaaatattt caaaaatata taacttttaa ctctttttaa attaaatggt
      901 tgttctgatc agaaaaatat cgcttgtttt cttattatgt aatatatttc ataaactatt
      961 ctgcataaca actattgttt gtacttttta cattggataa ctaattatga ctgctgataa
     1021 cttttgatat taaactagta tctccaatac aaataaaaaa tagatatctt gttcatcaaa
     1081 attagttcgt ggatacttac cttcatttac atcattcagc ggtttcccat tttcttttat
     1141 tctattattt cattttctaa tagacttggc ttcatttcgc aatacttttt ttcaataggc
     1201 atcaaattgg tgttgtttag atatttagtt gtagttttgt gacatgataa gtatgctcct
     1261 ttttctcttt tccactaata caacgatcaa agggtgcttt tttcaaaaaa ctagaaaaat
     1321 caaaataaga ttcgtatttt gattctatat atcctgtttc aactacatct tatatttcac
     1381 aatagaataa atagcctttt gcatagctgc ttaacttata agaaattgca atttacttct
     1441 tcaaagtgaa
//
LOCUS       DAZKCY010000252         1445 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000252 DAZKCY010000000
VERSION     DAZKCY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.263"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..686
                     /locus_tag="ACG8XC_002906"
     CDS             <1..686
                     /locus_tag="ACG8XC_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFP9192500.1"
                     /translation="ESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITL
                     FQPFMIIWVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAM
                     INIVLNIILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISL
                     CVIAFVASYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILK
                     VLTSVLVKR"
     gene            864..1085
                     /locus_tag="ACG8XC_002907"
     CDS             864..1085
                     /locus_tag="ACG8XC_002907"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFP9192501.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          498 a          138 c          297 g          512 t
ORIGIN      
        1 atgaatcatg tatagcaggt ataggtaaca gtcttattgt agaaagtcaa gaaaaaaatt
       61 atatagattt ttgtgatatt acaacaatta taggttggtt tctggcaatt agttgtgcat
      121 gctttattac attatttcag ccgttcatga ttatctgggt gggaagtgat aatcttatga
      181 gtatttcatt tgttatattt atgatagttc attttttctt atatgaaatt gatcagttaa
      241 ttgggttata caaagatgct gctggaatat ggtacgcaga caggtttaga ccattaatta
      301 cagcaatgat aaacattgta ttaaatatca tcctcgttaa ttggttcgga ttatatggag
      361 ttttggcggc aaccattgtt tcagtattgt tggttgattt accctggtta ttatataatg
      421 ttttccaaaa tatatttaaa tttgaatcgg caggtaaata tgtttggaag ctcataagtt
      481 tgtgtgtaat agcttttgtt gcttcttatt taagttattt tgcttgttcg tatgttaata
      541 tcggaaattt caaagtgata agtttagtga taaatttagt tatatcggtt gtcatatcga
      601 ttttagttat tcttctgttt aatatccgta ataagaagtt taagtatatt ttgaaagttc
      661 ttacgtcggt attggtaaaa agatgagaag agacatcaga atgatatata aagtaactac
      721 aatttttagg ctgccgaata ggcgaacgat gtatagtttt tgttccaacg aagactcttg
      781 ttgaaattgg agatgatatg aaggagttac aattttgact catgtttatg attgatcggt
      841 tttaaaagta gtatggagaa gctttgggat catcggagag gatcaaaata ggtaataatg
      901 tttttatagg aatgaagtct agaatactaa agggtgtaca tataggaaat aatgtagtta
      961 taggtgcaaa tagtttagta aacaaggacg tatcagacga ttgtgttgtt gcaggaaatc
     1021 tagcaaaagt aattatgtcc ctagatgaat attataaaaa gataaacaat atgaagaatc
     1081 cgtaaaactt gtgaaactat accgagaata atatgggaaa aatattgatg aaaaagcatt
     1141 atatgagttt ttagggctgt ttacagatgg agatgacaat ttgtcgaaga gttgggagca
     1201 atgcaaaatt tagtagggga ttataaacag actgacaagg tatttaaaga acatgcacaa
     1261 gtatttcata atattgaaga atttttgaat actgtgagat aggagttgct acattgagaa
     1321 aagtatgtat ttttccaatg taaaagatta cgagtttgaa aacgacacca taatattatt
     1381 ttttaagaag atattataga gcttaggaac ggaagtttat ttaaggatgt tgaataaaat
     1441 gaaaa
//
LOCUS       DAZKCY010000253         1429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000253 DAZKCY010000000
VERSION     DAZKCY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.264"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1429)
                     /locus_tag="ACG8XC_002908"
     CDS             complement(<1..>1429)
                     /locus_tag="ACG8XC_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9192502.1"
                     /translation="DELIRLTGNMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEK
                     LPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVL
                     INGATGVGKSFLATKIFEYAIHEQIIEEDAPFAILNCADYADNPELLSATLFGYKKGA
                     FTGAEKDTEGLLATANNGYLFLDEVHRLSKENQEKLFVFIDTGNYRPVGENVNWHSAK
                     VRFIFATTEKSEDYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFFQNEADILQKD
                     IIVSKEALSLMLSHPFSGNIGKLKNIIKISCADVYSRTREEPLTIGKNEILIQLNMLE
                     SEVESLPIASLLIKKNQKIKTDRIKPFDYDSILEKITQLINEGNFNEKFSSIKNEVQH
                     LVHHIDKDSFLSMSRKLMIQMFRKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKT
                     DLTGINRTLAANLPRTTYLCNQFLEH"
BASE COUNT          425 a          316 c          225 g          463 t
ORIGIN      
        1 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
       61 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
      121 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
      181 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
      241 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aacttttcat tgaaattacc
      301 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
      361 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
      421 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggctc
      481 ttcccgtgtt cgactgtaca catccgcaca actgatttta atgatattct tcagcttacc
      541 aatattccca ctaaagggat gagataacat tagagacaaa gcttcttttg aaacaataat
      601 atctttttgt aaaatatccg cttcattttg aaaaaataat tgaatcagct ctaaccgttc
      661 tcgaatggga cgatcttcaa aagttggcaa cataacactg atttgaatac gtcgatcaaa
      721 ggtgtctaat aaataatctt cacttttttc cgtcgtggcg aagataaaac gaaccttagc
      781 agaatgccag tttacatttt caccgactgg acgataattt ccagtatcaa taaaaacaaa
      841 gagcttttct tgattttctt tcgacaaacg atggacctca tcaagaaaaa gatagccatt
      901 gttggctgtt gccaataacc cctctgtatc cttctcagcc cccgtaaagg cccctttctt
      961 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacaatttaa
     1021 aatagcaaat ggtgcatctt cttcaataat ttgctcgtgg atagcgtatt caaaaatttt
     1081 tgtggctaaa aaactttttc caacccccgt ggctccattt attaatacac tgagtccgtt
     1141 aggcggatat ttgacagctg caatacattt ttgaatgacc tctctcagac ttccgtcatg
     1201 acccaccaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
     1261 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
     1321 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
     1381 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatca
//
LOCUS       DAZKCY010000254         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000254 DAZKCY010000000
VERSION     DAZKCY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.265"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(384..998)
                     /locus_tag="ACG8XC_002909"
     CDS             complement(384..998)
                     /locus_tag="ACG8XC_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9192503.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNRANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          464 a          276 c          235 g          442 t
ORIGIN      
        1 tcaggtaaac ttcattgcaa taatgataac aaatgtacta taattacttt gtaaagagtt
       61 ttggggaaga ctctttcatc aacgtgtatt aagagacaag aaagaatcca tatggattct
      121 ttcttgtctc tttttccact actttttatt tttctgacac tacatattct tataactcat
      181 ttctcataac tgtgaaacaa cttataaaaa taacaaactt taaacaaaac aagcttaaaa
      241 tcaaagatga tccatcttaa aataactaaa acaaaaaaag tagtcgttgt taatcctcat
      301 taataatcgt tttctttttt tcaaacgctc catttttatg tggaacatta tttttttaag
      361 gaactttgtc aacatgtgga taactatttt ccacttgtgt cctggactag ctgttttgct
      421 gtaaaataca aaacttgctg cagcaaaaag atcgttgcaa gagaggctgt tccgcagatc
      481 gctggatcta gatttggcaa agtcaagact gcatcatgta aattggcgcg gttgtccaaa
      541 accaaatcag caaattcaaa taatcgcaaa ccactgctat gttgcgattt gatacttcgt
      601 gacaaatcaa atccagtaac aatgattaat ggatgaccat tttctttgat ccactgagct
      661 agttcgatga ttgccgggtt tcttccttca ttcgaaagaa gaagaaaaat gtcttctgat
      721 ttgacagaaa gctgatcaat aattgcctgt cctgcacctt ctagcgtttc atagacacct
      781 ccggctggat tttcaatcaa tatacttggt acaagatccg gggtttgatc ccaaaattca
      841 aatgctacac cacttagtgt gcggctagca tatatctgca atctaccacc ttgattaatt
      901 tgtttcgcga tccaagtgcc agcttgaagc atcagcggct cttctatcct taatttctct
      961 tccaattggt gataatagtc aaaataacat aaactcattt atctctcccg cctagctata
     1021 cttgacatga atagaaacag ctgcctaatc taaggaagta cataaaaaaa cagtagttag
     1081 acagtaacac atgtggtctt tgctgtttca aacacaaaaa aggcattgaa cgaatacgcc
     1141 caataccttg acatagcaaa taaaacaacg tgcagaccat ggaaaatatc tacatagggg
     1201 tagaattttc accctagatt aggtacaata aattaggtcg atgcactgca ttgactatgg
     1261 caaaatgatc ggtcagggtg tatgtgtgtg actggtcatt ccctgattct tagcgccaac
     1321 taagggtcag ggagcctttt tttattcagc tgtttctatc tgaatttatt gtaagtaatc
     1381 tcttatccaa gtgcaagcaa gaaaatacaa aaaaaca
//
LOCUS       DAZKCY010000255         1355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000255 DAZKCY010000000
VERSION     DAZKCY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.266"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACG8XC_002910"
     rRNA            <1..1355
                     /locus_tag="ACG8XC_002910"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAZKCY010000256         1355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000256 DAZKCY010000000
VERSION     DAZKCY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.267"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..1117
                     /locus_tag="ACG8XC_002911"
     CDS             164..1117
                     /locus_tag="ACG8XC_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9192504.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1089..>1355
                     /gene="prgO"
                     /locus_tag="ACG8XC_002912"
     CDS             1089..>1355
                     /gene="prgO"
                     /locus_tag="ACG8XC_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFP9192505.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRS"
BASE COUNT          491 a          202 c          249 g          413 t
ORIGIN      
        1 tgaatatatc tgatatactg aatatagcaa atatatttag tatatccact atattcctta
       61 taaagtctga attcttatca atttggagta tattagatat atcgaatata ttaagtatat
      121 gaagtatact aaatatattt agtataggaa attggaggga actatggaaa acttacggac
      181 tgcatttaaa acatcagata aactaggaaa agttgtaatg cttacaggta gaaaaggcgg
      241 cattggtaag actacagaca atgatttatt agcgattgtt tctagtcaac tttttgaaaa
      301 agacgtgttg ctaattgatt atgaccaaca aagaaatact acttccaaca ttggatcaac
      361 gtatcaaatt actagctttg atcgatccat gtctgcagca atcaaaaaag gggactgggt
      421 ttcaggtatt actcaagtaa gtccacattt atatattatg gctggctctc ctggatctga
      481 agaacttaat gaatacttgt ccgaaaaata tcctgatcgt cgtaagagat cattggcctt
      541 cattaaacct ttagaagaat tgagaaaaaa tttcgattat atctttatcg actgcccacc
      601 atcaacagat aatgtggttc gtgcattctt aactgctgca gattatatta ttgcaatgca
      661 agaattaaaa cggtacgcaa tggaaggaac agaagatttt attaataaag ttttagttcc
      721 gattgtcacg aattttgaag agtctcatct tcaaatcatt ggaattttgc ctgttctttt
      781 ctcagtgcga cgttcctctc aacacgcaaa ctatcaaaaa acaattgata agtatggtga
      841 atcaaacatt tttaaaagca ttgttaaagg ctcggatcgg ttagaaatgt atggtgagaa
      901 tggtatccaa ttaaatgatt atgtagatcg aagatggtgg gctatttttg cagacatctt
      961 tacagaatta gaagaaagaa ttgaatacta tgaacaacat ggtgatattg aaggatttga
     1021 ttatacacct aaatatgctg actcaatggc caatcatatc ctacctcttg gaaaggagat
     1081 taaactaaat ggcattatta ccaacgaaca aagataagaa gaaaattggt gctgaagata
     1141 tttcaattaa agatgatatt aatgtcgagt taggggaacg aaagtacact gcgaaagaaa
     1201 gaaaaccagt tcaagtagat cctcccgtac taaaaaccat tcgggatctt agttatgcaa
     1261 aagatattcc tatgtatgaa atagtcaaac ttgcaatgga cgcctatgtt gatactttaa
     1321 acgacgagga acggattata tataatagac gatca
//
LOCUS       DAZKCY010000257         1329 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000257 DAZKCY010000000
VERSION     DAZKCY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.268"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..722
                     /locus_tag="ACG8XC_002913"
                     /pseudo
     CDS             21..722
                     /locus_tag="ACG8XC_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            735..1193
                     /locus_tag="ACG8XC_002914"
     CDS             735..1193
                     /locus_tag="ACG8XC_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9192506.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT          490 a          185 c          267 g          387 t
ORIGIN      
        1 acttaatttg acaattaaga gataaagaat tgcaacgaga aattatgcca gagattattg
       61 aaaaactaat aacaaaagaa atgcaagtcg ccgattttaa tataggagat aaaacttggc
      121 aaaaaacctt tcaaatgatg tcagtcatca cgaaaaattt tccaagtaat atcccgaata
      181 attacttaga gtattactta tatcctgata tggtcgttga gcatactgac aaagagtata
      241 cacgtgctaa tatggtcatg gatggacgag agaaaaatac caaagaaatg gctgataaaa
      301 tacgccgcgg tattgtggaa gaagtattga actttaattt tggggagcat ggacaatata
      361 tcgtcgatat agctatttcc ttgttgaatg atgatcgtcg tcgctttatg ctaatcgttc
      421 ctaatcaagg ggcgattccg aatctacggt cagatgcagt tgttgaaata ccggcatatg
      481 ttggtgcaac tggtgtggag ccaattacac taagaaaacc aatcagtgat tttcataaag
      541 gattgatgga ggcacaagtt gcagtagaga agttactagt agatgcttac tttgaaggtt
      601 cttatcaaaa agctttacaa gcatttacat tgaatcaaac cgtaccaaat gccagagtcg
      661 caaaaaaaat actagatgaa atgatcaaag taaacaaaaa atattggcca gcattaaagt
      721 agaggtgata aaaagtgcat ttctttaaaa caagaaacat tctaaaagct gtagctagtg
      781 gtcaaattat tccattaaca gcagtaaaag acgaggtctt ttcttcaaaa atgataggag
      841 ttggatacgc tataacgaat catgatggat atatccatgc gccagttaaa ggaataatcg
      901 ataatatatt tcctacccga catgccataa cgatcgaaag tgaatctggc agaaaaatac
      961 tgatccatat gggtttagat acagttgaac tcgaaggaag tccgtttcag atacgggttg
     1021 aaagtaacca ggaggtaaaa gaagggacac tgttagctat tatagatttg aagaagttgg
     1081 agcaagcaca aaaagatacg gtgatcattg tagtttctcc aggcattttt gaaggaaatt
     1141 tattaaaaca aaaccaagaa gtaaagatta aagatagttg ttttgaattt tagtgtacga
     1201 tgaaaaaagt taaatagggt gatatttatg ggtgaattta caaaattttt ttgaaatgat
     1261 atgtttcaat cactagattt aatcattttg atttttgtta attatcgaat ttttttgttg
     1321 ctcctatga
//
LOCUS       DAZKCY010000258         1318 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000258 DAZKCY010000000
VERSION     DAZKCY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.269"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1318
                     /locus_tag="ACG8XC_002915"
     CDS             82..>1318
                     /locus_tag="ACG8XC_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFP9192507.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFK"
BASE COUNT          447 a          257 c          251 g          363 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaat
//
LOCUS       DAZKCY010000259         1318 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000259 DAZKCY010000000
VERSION     DAZKCY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.270"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..1228
                     /gene="xerS"
                     /locus_tag="ACG8XC_002916"
     CDS             143..1228
                     /gene="xerS"
                     /locus_tag="ACG8XC_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFP9192508.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          526 a          219 c          195 g          378 t
ORIGIN      
        1 tattaaatct atacctattt atctgctttt ttgaaataaa ttaataaatt tcagagtata
       61 ccaattgaca aaacactcta taattggtat agtccaatta tagagatact cctctcccct
      121 acttatcgaa aggatgaata acatgaaaaa tacagaaata tatcaaaaaa tagatacaat
      181 catacttcat atgcccgatt atataaaaga ttttgttcaa gatcgtgagg ataaagatca
      241 atcacctcga actactttag aatatctaaa aaattataag ctcttttatg aatggcttct
      301 gagtgagtct atcgttcccg atacttcaat cacctcgata agacagttaa ctgcctatga
      361 tctaaacttg tacaaatctc atttgaaacg tcgcacaaag gaaaatgtaa aaaaagagac
      421 aacaaaaact aagctcaaag ataataataa cttaggtcta tctacttcta ctatcaatag
      481 aaatatcaca gcattaaagg ttctatttaa atatctttcg aaaagttcca ataatccttt
      541 aggaaaacca tacttagaaa ataatcctat ggatcaagta gcaactatca cagataaaac
      601 tacgttagct gcccgtgcaa acgcaatcga gaaaaaatta tttctagacg aagatacaca
      661 aaattatcta gattatattg ccaatgaata taaaaaaact ttgagcaaac gagctttgat
      721 ctattatcaa cgagatgtag aaagagacct ggcgatcaat gcattgattt taggatctgg
      781 attaagactc tccgaggttg taaatattaa tttagaggac ctatctttag aaaagaataa
      841 tgtggtagta actagaaaag gaaataagag agacgcagta aatatagcag catttgcaat
      901 ggaatattta gctaattatc tagctattcg gaaagaacga tacaaagtaa cagaaaacga
      961 aaaagcttta tttctagcaa tttatcaagg agaagccaag cgtatatccg gtattgcaat
     1021 cgaacggatg gtagcaaaat attcaaaagg attcagagtc caagtcagtc cacataaatt
     1081 acgtcatact ttggctacta gactctatca acagacaaat tcattggtat taactgccca
     1141 acaattggga catacaagta caaatacaac aactttatat actcatatag acaatgcagc
     1201 tacaattgat gctttgaacg ctttgtaaat gtagtaaaaa atagtataat aatgaagcct
     1261 gtctctaaaa ttagataaaa gcccaacttt aaaggcaagc ttcattatca tttttata
//
LOCUS       DAZKCY010000260         1311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000260 DAZKCY010000000
VERSION     DAZKCY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.271"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG8XC_002917"
     CDS             200..742
                     /locus_tag="ACG8XC_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192509.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1311
                     /locus_tag="ACG8XC_002918"
     CDS             898..>1311
                     /locus_tag="ACG8XC_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFP9192510.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMA"
BASE COUNT          532 a          148 c          255 g          376 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DAZKCY010000261         1290 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000261 DAZKCY010000000
VERSION     DAZKCY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.272"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1290
                     /locus_tag="ACG8XC_002919"
     CDS             82..>1290
                     /locus_tag="ACG8XC_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFP9192511.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKT"
BASE COUNT          434 a          246 c          248 g          362 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca
//
LOCUS       DAZKCY010000262         1289 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000262 DAZKCY010000000
VERSION     DAZKCY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.273"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            394..>1289
                     /locus_tag="ACG8XC_002920"
     CDS             394..>1289
                     /locus_tag="ACG8XC_002920"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9192512.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSST"
BASE COUNT          508 a          193 c          252 g          336 t
ORIGIN      
        1 ggtagtttta atatattcat ttttagttgt ataaaaatat atcataataa aaacaacaaa
       61 aaaatgtctg gaaaatcgaa cataaaagtg tctgatttga atatcccagc agcggaagaa
      121 gaaaaacaaa atcgataaca tttgttctaa gaattagtta cgttaaatac ggaacgcaag
      181 atagacttga aaaacggtaa caaaagaagt agtatattaa ggtgtgatgt gctacttgac
      241 atttattctg ctgagcgaac tttctggatt gtgtagcctt atatctattc aggacgtatc
      301 gagcagatta gtaaaggtgt tcttgatgat tttcatcttg aacacctttg tttttagcaa
      361 ttcgcaaaaa acgaaaagta ggagaattga taaatgaaaa aaagattttt agcacttgcc
      421 atcgtactag gcactggctt attgtcaggc tgtacgaatg ccggtgaaaa aacagccgtt
      481 agttataaag gcggtacaat cagcgaacaa gaagtaatgg atagcttgaa aaaaatgcaa
      541 ggagcagatt ccgctgtgca acagttgatc gtttatcaag tatttgaaga taaatatgga
      601 gatgacgtat caaccaaaga aatcgattct caatatgatc aaacaaagaa acaattgggt
      661 gattcatttg attcacaatt gaaatctgca ggttacacag aacaaacatt caaagacagt
      721 atcaaacaaa gtctagcctt tcaagaaggt ctaaagaaac atatcaaatt gaccgatgaa
      781 gatttaaaaa cggcttggga atctttccat ccagaagtgg aagctcaaat tatccaagta
      841 gcaagtgaag atgatgcaaa agatgtgaag aaagctgctg ataagggaga cgatttctct
      901 aaattagcca aagacaagtc aactgatact acgacaaaag aagatggcgg aaaagtgaaa
      961 tttgattcca ctactacaac ggttccggca gaagtcaaag aagcagcctt taagttgaaa
     1021 gatggtcaag tttctgatgt gatcacttct accaatgctt caacttatac tacggaatat
     1081 tatgtggtta aaatggtgaa aaaccaaaac aaaggcaacg acatggataa atacaaaaaa
     1141 gaattgaaag aaattgcaac agacacaaaa ttaagtgaca gtaccttcca gaataaagta
     1201 attggcgaag tattgaaaga cgctaatgta aaaatcaaag acaaagactt tgaaaatgtt
     1261 ttgagtacat tcacttcaga ttctagtac
//
LOCUS       DAZKCY010000263         1288 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000263 DAZKCY010000000
VERSION     DAZKCY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.274"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACG8XC_002921"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACG8XC_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACG8XC_002922"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACG8XC_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          456 a          217 c          175 g          440 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaa
//
LOCUS       DAZKCY010000264         1286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000264 DAZKCY010000000
VERSION     DAZKCY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.275"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1154)
                     /locus_tag="ACG8XC_002923"
     CDS             complement(15..1154)
                     /locus_tag="ACG8XC_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFP9192513.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          335 a          281 c          239 g          431 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tatttt
//
LOCUS       DAZKCY010000265         1280 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000265 DAZKCY010000000
VERSION     DAZKCY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.276"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..75
                     /locus_tag="ACG8XC_002924"
     CDS             <1..75
                     /locus_tag="ACG8XC_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFP9192514.1"
                     /translation="PETAIRMEIDQLNRQQRLFGDDNE"
     gene            81..245
                     /locus_tag="ACG8XC_002925"
                     /pseudo
     CDS             81..245
                     /locus_tag="ACG8XC_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
     gene            248..475
                     /locus_tag="ACG8XC_002926"
     CDS             248..475
                     /locus_tag="ACG8XC_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192515.1"
                     /translation="MINLNAVAFEQTWRTKYKKMSPRDKLFLEIMTFAFIGTQAEQSD
                     ISVEKIKTNRLVNGITETCYQYTIIVVDEEE"
     gene            478..>1280
                     /locus_tag="ACG8XC_002927"
     CDS             478..>1280
                     /locus_tag="ACG8XC_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replisome organizer"
                     /protein_id="HFP9192516.1"
                     /translation="MAERRMFAKTIIDSDAFLDMPLSTQALYFHLSMRADDDGFINNP
                     KKIQRMVGCGDDDLKLLMAKRFILVFESGVIVIKHWKIHNYIRNDRYKPTLYQDEKAL
                     LADKDNKAYTFAEELSKHDEKLGIPDDNQAVYQMDTQVRLGKDRLGKDSKEIKDLTPS
                     KKSKAKPIRHKYGEYKNVLLSDEQMEKLKTEFPNDYQERIERLSEYCESSGKTYKNYL
                     ATIRSWARKEKSEPKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERA"
BASE COUNT          493 a          204 c          271 g          312 t
ORIGIN      
        1 ccagaaactg cgatccgcat ggaaatcgat caactgaaca gacaacagag actttttggt
       61 gatgacaatg aatagagctg aagcgcaaag aagttgatca ttcagagtac acatttgcag
      121 cactctgtag aattcatcac acggagaaac acaaaatagg tgtgaccaat ttcaaaaata
      181 agtatcaaat caaagggatt aagttaaacc aggagacaat caagaaatta agaatcgggg
      241 gctaatgatg attaatttaa acgcagtagc gtttgaacaa acatggcgca ctaagtataa
      301 aaaaatgagt ccaagagaca aattgttctt agaaatcatg acatttgctt tcattggtac
      361 acaagctgaa caaagcgata ttagtgttga aaaaattaag acgaatagat tagttaatgg
      421 aattacagag acttgttacc agtacacgat tatcgtcgtg gatgaggagg aataattttg
      481 gcagaaagaa gaatgttcgc aaagacaatt attgattctg atgcattcct agatatgcct
      541 ttatcgacac aggcattata tttccatcta tcaatgcgcg cagatgatga tggatttatt
      601 aataatccca aaaaaataca acgtatggtt ggttgtggtg atgatgattt aaaactgctt
      661 atggcaaaaa gattcatctt agttttcgag agcggtgtga tcgtcattaa gcattggaaa
      721 atacacaact atattcgtaa tgatcgttac aaacccacgt tatatcagga tgaaaaagct
      781 ttactagcag acaaagataa caaagcttat acatttgcag aagagctttc taaacatgat
      841 gaaaaacttg gtataccaga tgacaaccaa gcggtatacc aaatggatac acaggttagg
      901 ttaggtaagg ataggttagg taaggatagt aaagagataa aagatttaac gccttcgaaa
      961 aaatcgaagg ctaagcccat ccgtcataaa tacggagagt ataaaaatgt tcttttgtca
     1021 gatgagcaga tggagaaact caaaacagaa ttccctaatg attaccaaga gcgaatcgaa
     1081 cgactgtcag agtattgtga atcatctggt aagacttata aaaactattt ggcaactatt
     1141 cgaagttggg caaggaaaga aaaaagtgaa cctaagaacg caagcagtgg atacaagcgc
     1201 acaggaagac gagagaagct tcctgaatgg gcaatcgacc aagaagccta tctcaagaaa
     1261 aaagcgctag aacgagctaa
//
LOCUS       DAZKCY010000266         1275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000266 DAZKCY010000000
VERSION     DAZKCY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.277"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            145..1017
                     /gene="scrK"
                     /locus_tag="ACG8XC_002928"
     CDS             145..1017
                     /gene="scrK"
                     /locus_tag="ACG8XC_002928"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFP9192517.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          391 a          227 c          290 g          367 t
ORIGIN      
        1 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
       61 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
      121 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
      181 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
      241 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
      301 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
      361 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
      421 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
      481 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
      541 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
      601 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
      661 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
      721 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
      781 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
      841 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
      901 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
      961 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     1021 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     1081 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     1141 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     1201 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     1261 atatctttgc ttctt
//
LOCUS       DAZKCY010000267         1251 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000267 DAZKCY010000000
VERSION     DAZKCY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.278"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG8XC_002929"
     CDS             73..234
                     /locus_tag="ACG8XC_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9192518.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>1251
                     /locus_tag="ACG8XC_002930"
     CDS             253..>1251
                     /locus_tag="ACG8XC_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9192519.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQ"
BASE COUNT          517 a          111 c          216 g          407 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca a
//
LOCUS       DAZKCY010000268         1233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000268 DAZKCY010000000
VERSION     DAZKCY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.279"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..979
                     /locus_tag="ACG8XC_002931"
     CDS             <1..979
                     /locus_tag="ACG8XC_002931"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9192520.1"
                     /translation="EMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLF
                     DKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTIC
                     LSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNE
                     FDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWI
                     NSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQAD
                     FDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            961..>1233
                     /locus_tag="ACG8XC_002932"
     CDS             961..>1233
                     /locus_tag="ACG8XC_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9192521.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNN"
BASE COUNT          436 a          168 c          237 g          392 t
ORIGIN      
        1 agaaatgaga caactacaca aagaacagat tgaaacagca atggcagcgt taaaggaaaa
       61 gacaattgat catgtatatt ttacagcatg tgggggttca caagccgttt taatggctgg
      121 tcaatatcta tttgataaag aattaactgt accttgtcat gtttatactg ctaacgaatt
      181 taactatgct ataccaaaag gattatctga aaattcttta gttatcactt gttcccattc
      241 tggcacaaca ccagaaactg tagaagcaac aaaaaaagca agagaatttg gagcaatgac
      301 tatttgttta tctaatgaag aaggatctcc tctatgggaa gcagctgaat atcctgttca
      361 ctatgattgg ggtaaagatg ttgatgctag tgatttaaat aaaggaattc tttatggttt
      421 attatttaat attttacttg ttttagatgg ctctaaaaaa tgggaaactt gcttgaatga
      481 atttgataag ttaaatgatc ttgttattgc tgaaaagagc aaatttgaaa taactgctaa
      541 agaatggggt aaaaaccaaa aacgagataa aattatttac acaattggta gcggtatcaa
      601 ttacggagaa atttactcca cagcagcttg ctggttcatg gaaatgcaat ggattaattc
      661 taatgctatt cattctgggg aatttttcca tggtcctttt gaaatcactg attatgatgt
      721 accattcatc ttagtgaaaa gtattggaga tacaagattt ttagatcaac gggtagaaga
      781 cttcactaaa aaattcagta ataaattaat ggttttagac caagctgatt ttgacttaga
      841 atctatagat agtgtagcga aagaatatat tgcagctgta cttagtggcg ttgttattcg
      901 ccaaatggta gaagcgattg cttttgaaag aggccattcc ttagatgtta gacgctatat
      961 gtggcaaatg aaatattaat taataggagg ttgcaaaaga tggcaaatca attatttcac
     1021 attgtgatta caacacattc taccctttgt gagggttatt taaaggcaac aaaattaatt
     1081 ttaatgaatg acgccacagg tattagtgct gttccattta aagaaaatac tagtccagat
     1141 gattttgagg agagtatgaa agaaataatt agtcagcatc atttgcaacc tctaattatt
     1201 ttaacggatt taattggagg aacacctaat aat
//
LOCUS       DAZKCY010000269         1230 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000269 DAZKCY010000000
VERSION     DAZKCY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.280"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1129
                     /locus_tag="ACG8XC_002933"
     CDS             <1..1129
                     /locus_tag="ACG8XC_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFP9192522.1"
                     /translation="LNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGS
                     YFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIAD
                     LIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIPLKRQTVSKE
                     AIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLLFITDGLKGMKD
                     TIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMI
                     EKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQ
                     NEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          448 a          224 c          202 g          356 t
ORIGIN      
        1 tttaaatgaa ttattccgtt cgcacttaga aaaagcgata aacacactcc tacggactga
       61 attaacggct tttttagatt acgaaaaata tgatcgcact ggttttaatt caggtaattc
      121 gagaaacggt tcttactttc gatcaatcaa aaccgaatat ggtgaattaa cattggaaat
      181 acctagagat cgtaatggtg agtttaaaca acaaacttta ccagcctaca aaagaacaaa
      241 cgatacattg gaaaccacta ttatccattt attcgaaaaa ggtgttacga tgtctgaaat
      301 tgctgatttg atcgaaaaaa tgtacggtca tcactatact ccacaaacca tgtccaacat
      361 gactaaagtt ctgactgaag aagtaaatgc ctttaaatcc agagccttaa atgataagta
      421 tgtcgctatt tttatggacg ctacttacat tccactaaaa cgtcaaaccg tatccaaaga
      481 agcgatttat attgccattg gtatacgaga agacggcact aaagaagtac tgagttatgc
      541 gattgctcca actgaatcaa catacgtttg gaatgagctg ctacaggata ttaactccag
      601 aggagttcaa gaagtcttgc tttttattac ggacggctta aaaggcatga aagatactat
      661 ccatcaaatt tatcctaaag caaaatatca gcattgttgt atccatgtat ctcgtaacat
      721 cgctcataaa gtacgtgtca aagaccgaaa agaaatctgt gatgacttta aggctgttta
      781 tcaagctaac tcaaaagaag aagcgaatac cttcttatcc ggcatgattg agaaatggaa
      841 gaaaaactat cctaaagtga cgcagtcact catagaaaac caagacttat taacttttta
      901 tgattttcca cctagcattc gtagaaccat ttactcaacc aatctaatcg agtctttcaa
      961 taagcaaatt aaaagataca gccgtagaaa agagcagttt caaaatgaag aatcactaga
     1021 acgctttcta gtcagcattt ttgatacata caatcaaaaa tttctaaaca gaagccataa
     1081 aggttttcaa caggtaaccg atacattagt ttcaatgttt actgagtaac taattatttt
     1141 gcaggaggac aatttattta cacaaaatta ttgacgctcc caattaggac tcaattgttc
     1201 atagatgagc atttgtaatt aagttatatt
//
LOCUS       DAZKCY010000270         1224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000270 DAZKCY010000000
VERSION     DAZKCY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.281"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1145
                     /locus_tag="ACG8XC_002934"
     CDS             <1..1145
                     /locus_tag="ACG8XC_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFP9192523.1"
                     /translation="EILINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFN
                     SGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKG
                     ITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPL
                     KRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVT
                     DGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLVYQASSKEEA
                     NNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEGFNKQLKKYT
                     KRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          444 a          183 c          206 g          391 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttcaaatta gtttaccaag caagttcaaa agaagaagca aataatcaaa
      841 taaggtttat gatagataaa tggaaaaagc agtatccacg agtggttaaa ttgctcatga
      901 atcctgctat attaaccttt tataatttcc ctccgtcaat cagaagaact atttattcaa
      961 ctaatttgat tgaaggtttt aacaaacaat taaagaaata taccaaaaga aaagagcaat
     1021 ttcctaatga agaatctctg gagagatttc tagtatctca attcaacaac tataatcaaa
     1081 agtttctatg ccgagttcat aaaggtttta aagaaataca tgatacatta gaatcaatgt
     1141 tttaacttga tcaacagaat gggcttttcc atttacacat aattattgac acaacctatt
     1201 ctggaaactt acaaataaaa gctt
//
LOCUS       DAZKCY010000271         1218 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000271 DAZKCY010000000
VERSION     DAZKCY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.282"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..844
                     /locus_tag="ACG8XC_002935"
     CDS             <1..844
                     /locus_tag="ACG8XC_002935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9192524.1"
                     /translation="HMSYRGSFSRKYVLNKWFRLLNIPVIIHLHGSEFKKWYDSVSVK
                     KQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENSIVVPEYTIKWNSKMCRF
                     LFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDEEISLKELIEEYNLQSYF
                     EFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYGMPVIATDVGDISSAVRD
                     GENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAITKFNDGNFFDRILNLYQK
                     GSKE"
BASE COUNT          456 a          124 c          256 g          382 t
ORIGIN      
        1 gcacatgtcg tacagaggga gttttagtag gaaatatgtg ctgaataagt ggtttagatt
       61 attaaatatt cctgtaatca tccatcttca tggatcggaa tttaaaaaat ggtatgatag
      121 tgtttctgta aaaaaacaaa atcagattcg cttgctgtta agagagtctg attccacgat
      181 tgtacttgga gaaaaatgga agaagataat taaagaaatt gagccaaatt gtaaaactgt
      241 tgttgtagaa aattctattg tagttccaga atataccata aagtggaatt caaaaatgtg
      301 ccgatttctg tttatgggag ttttgataaa gcgaaaaggt gtacacgatt tggtagaagc
      361 agttaaaaac attgcagaac aatatccaga aaaaaagttt atttttgaga ttgctggagc
      421 aggtgatgaa gaaatatcgc tcaaagaatt gatagaagaa tataatttgc aatcgtattt
      481 tgaatttaca ggttgggttg tgggagaaaa aaaggaggag tgctatagaa gatcaaatgt
      541 gatgatttta ccgtcctata acgagggatt gcctatatct attctagagg cgatttctta
      601 tggaatgcct gtaattgcta cagatgtagg agatatttct tcagcagtac gtgatggaga
      661 aaatggatat ttgtttcaac ctggtgacgt atcagcgttg acatctgcaa tactaaaaat
      721 caatgataaa gataaatacg taaaattttc acgttgttcg aaggatttgg caataacgaa
      781 atttaacgat ggaaatttct ttgatagaat tttaaatctg tatcaaaaag ggagcaagga
      841 gtgatgacaa ggaaaataaa aaatgtttcg attatgtaat gaacttatta agtgaataaa
      901 atagagagaa aaaatattga aatagacgat gtaaattttt atccgtgtaa gaattcaaat
      961 gaatagtagt gttttagtgc ttgtaataga gcaagagtac aagcacggta cattatataa
     1021 aaacattaat gatatttcta taaataaata gattttgtat tctggatgat gaagaggaag
     1081 atattatctg ataacttacc ctaaactttg cagggggtgg ttataagttg tgtatatgtg
     1141 gcagtaatga atgagtgaaa taatcatatt aagaaataaa attgcaatat tgtgtaatac
     1201 aaaagtatat tttcatta
//
LOCUS       DAZKCY010000272         1204 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000272 DAZKCY010000000
VERSION     DAZKCY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.284"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(95..421)
                     /locus_tag="ACG8XC_002936"
     CDS             complement(95..421)
                     /locus_tag="ACG8XC_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192525.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(408..1196)
                     /locus_tag="ACG8XC_002937"
     CDS             complement(408..1196)
                     /locus_tag="ACG8XC_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9192526.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          342 a          218 c          174 g          470 t
ORIGIN      
        1 acaatatgaa gataagcgac ttatcagctt tgatactact gatttttata aaaaaaatat
       61 cacgatatta tgatatcgtg atattgaatg taaattaaat tttattttgt ttctttgcta
      121 gtttatatcc ttcaatcaag tattcttcag gtatggactt taatagcgca tcttctataa
      181 cgtctggttt acttttgtct gattcagcaa tagtcgtcca atatttgagt gctgacatta
      241 gaattggatt tatacgaatg ctatctgctg ttcttacact acttccttga tcttttatga
      301 ctttacttat atttttcttg gttaattcga tttcttctac ttcttttgat ttaaaattct
      361 ttttattttt atttcctagt aatctactta ctgcagcttg actgcgatta tcttctgcca
      421 tcaaaatcac caacttcttt taaatattct tctgtaaatg ctttatagtc atcttcagct
      481 gttgagggtt tattaaaatt gattagatct gatccagcag cttgcgcttc gtcaacggtt
      541 attgtcctac gaatataagt tttgaaaatt tttgcaccaa tgactctaga aatttcggtt
      601 gctgcagacg tcatttcttt agaaatgtta taacgcttat tatacttagt aaatagaact
      661 cctcgtactt caagcttgtc gttcgtatat tcttttaccg tttcaatagt ttcacttaac
      721 tttattagtc cttttaatga gaaatacgca gcttcaactg gaatgataat ttcatcagaa
      781 acagtaaatg cgttgactgt aagcattcct aaacttggtg gacaatcaat tataatataa
      841 tcatagttgt ccttgattgg ttgaagtact cttttaagcc tgtgttcact tcctacttga
      901 gtaaattccc tctctgcaga agaaagggct atgtcggctg gcaaaacatc aattccttta
      961 tagtccagaa tagcatctgt aatgtcagcc tcccctttta atacttcata aattgtaggt
     1021 tggtttgttc catctatatc gaaacctaca ttatctgtag catttccctg aggatcaatg
     1081 tccactaaaa gtacattttt accgtaaaca aatttcaaac atgctgctaa attaacgcta
     1141 gtcgtagttt tagaaactcc acctttttga ttggctactg acaatattct tgccattaaa
     1201 atat
//
LOCUS       DAZKCY010000273         1195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000273 DAZKCY010000000
VERSION     DAZKCY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.285"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..566
                     /locus_tag="ACG8XC_002938"
     CDS             <1..566
                     /locus_tag="ACG8XC_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192527.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
     gene            complement(613..849)
                     /locus_tag="ACG8XC_002939"
     CDS             complement(613..849)
                     /locus_tag="ACG8XC_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192528.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            947..1126
                     /locus_tag="ACG8XC_002940"
     CDS             947..1126
                     /locus_tag="ACG8XC_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192529.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT          425 a          196 c          253 g          321 t
ORIGIN      
        1 aagaacaatt gtatttggaa agtgaaacga gtcaaacgaa ggtcacagtc agccataatc
       61 gtttagacgt gtataaacgt cgtcctgaaa cggtaaaaaa aggaacggag cttctacgac
      121 tagaaacaga aaaatacatc atgaatgttt acctagaaag gagtgagctg gtcgaaaact
      181 ttgtcagcta cacgatttgc aggaaacact attactcaga atcagaatat tattcaattt
      241 ttgagagcat ggaattaatg gaagaagaaa tggaagagct aaacagcaaa gatgcgacga
      301 tagacgatta ttaccttgta tttactagat ttttgacgtg tggaacggaa tctatcgtgt
      361 taatgggaat ggattatgaa ctcgctgtac ccgaagcatt tttgagggaa tatgtagaag
      421 aagtagataa cccaggcgac gactggtaca cgactttaac tgatttttta gacacctata
      481 cttacgacga tacagaagaa atccaaaatg aacttgaccg taaacgaatg ccatatcaac
      541 gatttgaaag gaaaaaaagg caataatcat ttataccgaa aagtgagaat gtgtgaaata
      601 aggacaaaaa acctagtaac taagggaatg gttcttattt cgcaccaatc gctccaaccg
      661 cccccgccgc ttaggtggtg cgaaataagc gggcaacacc cgataaccta agagaaagaa
      721 ggagatccat atgaataaaa tcattcctct attgttagtc ctatttgttg gcgaaatact
      781 tatgcgccat gctgcagctg tgcatttaaa cttcgtagta tttttggcaa tactggtatt
      841 ttgtttgatc ttttggcgct ttgtattagc ccttgtgagt ctactattca agatgattgc
      901 agccatattt ttcatagggt tatttcgatg aaaggagaga gtactaatgc caattgcaat
      961 aggaaataaa cggttacccg ttacgttgga tgaaaagaga caaaaagaat tgcagcaact
     1021 aaagcagaag tacggcaaaa gtgaatccag gattatgtgt attgcgttag atttattgat
     1081 tgcccaagaa aaagcaggat ttgaggtacc agcactcaaa aagtgacgtc actttttatc
     1141 ctaaaaacta aaagtgatag cacttttaat tataagaagt tagaatatta atcat
//
LOCUS       DAZKCY010000274         1143 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000274 DAZKCY010000000
VERSION     DAZKCY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.286"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(262..1110)
                     /locus_tag="ACG8XC_002941"
     CDS             complement(262..1110)
                     /locus_tag="ACG8XC_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9192530.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          366 a          191 c          197 g          389 t
ORIGIN      
        1 attttttaga aaataagaaa caaaaataat aaaaaaaaga aaagataaat tttttactac
       61 tgtatactaa ctttgttatc gcatctaaca agtaactcga ataataccaa tacaaccttc
      121 tccaaagatg cgatgacata cgttctaacc caccgactat tgatgttctg taccaccctt
      181 gcagaaccaa aaaagccact accaaatatt tggtagtggc tttttttgtt ttatggattt
      241 ttcgcttttt ctgttatggt gctattcttt gatttcagaa atagtatgtc gaactttagc
      301 gatagaacga cgtgattctt tttcgttgta aaacatcaaa atcacatata gagagtccaa
      361 aatgcttagt tgagaaatac gggaagctaa agcttcagat cggaattctg tttcttcaga
      421 aatagaaata aaaacgacat cgccaagttt agcaagggaa gaattagctt ggctagtaat
      481 aacgattgtt tttgctccag tttttcttac ggtttcagca atatggattg tttcttttga
      541 ttgccctgtg tgagaaatca aaacagcaca gtctttttcc gtcaataaag aagcttccat
      601 caattgaata tggtaatccg tgctgtgaga agttgcgata ggggaacgca aaaatttatg
      661 ataagcgtca gttgctacaa tctctgaacc gccaacaccg aaaaaataga cacgtttaga
      721 ttcattgata atagtagccg ctttttttag atccttttgt tgtaacaact tttttgtgtc
      781 atttaaagtt gaaatactag actcgaacac cttttgcgcc atagtgagct cagtatctgt
      841 tttctgaata ttttcgtgga tagaaatagc agatagatca ttttcttgga tcaacatggc
      901 cattttaaaa tctttaaatc cattataacc aagttttttt gtaaattgaa aaaaagtaga
      961 gtcagcaatg cccagttcac ttgctaaatt gctaatagga ctatgactta ctttgcttgg
     1021 attttctaaa atataatcgg cgatagcctg ttcttttggg ctgaagtcgt tgtacattgt
     1081 tttgatttgt agtcttatgg agacgctcat gagaattcct cattttcata ataagattat
     1141 cat
//
LOCUS       DAZKCY010000275         1143 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000275 DAZKCY010000000
VERSION     DAZKCY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.287"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..>1143
                     /locus_tag="ACG8XC_002942"
     CDS             33..>1143
                     /locus_tag="ACG8XC_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFP9192531.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQK"
BASE COUNT          346 a          238 c          253 g          306 t
ORIGIN      
        1 cacaacacat tcacagaaga gaggacttcc ctatgaacga ttttactaca gaaattctaa
       61 agactctagc gaacaaaggc gatttgaatg aattattccg tgtccatttg gagaaagcag
      121 tcaatacgct tctcaaaacg gagttaacgg ctttcctaga ttacgaaaag tatgatcgca
      181 ttggttttaa cacgggtaat tctcgtaacg gctcctatga ccgtacggtc aagaccgagt
      241 atggggaact tcatctccag attccgcgcg accgcaatgg tgagttcaag caacagactg
      301 ttcctgctta tagacggacg aatgacacgt tagaggagac cgtcattcac ctcttccgaa
      361 aaggtattac catgtcggaa atcgcagact tgattgagaa aatgtatggg cattactaca
      421 cgccccaaac catgtccaat ataacaaaat catttacaga agaggtaacg gcgtttaatg
      481 ggcgggagct tcatgaccgt tatgctgcta tttatatgga cgcaacgtat attccgttaa
      541 agcggaaaac cgtcgccaag gaagctattc atatcgcagt tggcattcgc ccggacggat
      601 caaaggaagt attgagctat gcgattgcac cgactgaatc catcacgatt tgggaggaaa
      661 ttttattgga ccttcaagag cgcggtttga aaaatgtcct cctgttcatc acggatggct
      721 taaaggggat ggtaggagcg attagtcggt tctatcccaa agctcgtttt caacattgtt
      781 gtgtacacgt ttcccgtaat atcagtcata aagtgcgtgt cgatgatcgt aaggaagtct
      841 gtgatgattt taaaatggtg tatcaagcct catctaaaga ggtggcgttg gaagcacgtg
      901 gtacttttgc ggaaaaatgg aaaaccagct atccaaaagt ggttgaatcg attctttcga
      961 acgatcactt gctcactttc tatgatttcc ccttggccat acgcaagagt atttattcta
     1021 cgaacttgat tgaatccttt aataaacaaa tcaagaaata cagccaccgc aaggaacagt
     1081 tccaaaatga agagtcgatg gaacgtttct tagtctcgtc ttttgatact tacaaccaaa
     1141 aat
//
LOCUS       DAZKCY010000276         1123 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000276 DAZKCY010000000
VERSION     DAZKCY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.288"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..487
                     /locus_tag="ACG8XC_002943"
     CDS             <1..487
                     /locus_tag="ACG8XC_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFP9192532.1"
                     /translation="PVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSR
                     GMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGD
                     GKYEYTIYSSCCNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYI
                     N"
BASE COUNT          376 a          165 c          178 g          404 t
ORIGIN      
        1 ccctgtgggc gttcttacca ataatccttc atttgactat caattattta atttaaacaa
       61 ttatcgtgtc ttatcgagtg aaactcctaa aaataatttt tcaaatcaaa taagtttgaa
      121 tgcctatagc cgcggtatgg gagggatagg cttgcctgga gatttatcct cagtatctcg
      181 ttttgttaaa gcgactttta cgaagctgaa ttctgtatct ggagattcag agtcagaaag
      241 tattagtcaa tttttccata tcttaggttc agtagaacaa caaaaaggtt tgtgtgatgt
      301 tggtgatgga aaatatgaat atacaattta ttcttcttgt tgcaatgttg acaaaggaat
      361 ctattattat cgaacatatg aagacagtca aattactgca attgatatga ataaagaaga
      421 cttagatagt cataagttaa ttagttatcc aattatagaa aaacaacaaa ttaaatatat
      481 aaattagtta atgtgttgtg attgattatt taatatagat ataatacaaa aaggcaaaga
      541 tattttcaaa acattttttc ttgatgtgat atccttgtct tttttgtaat gtttttaact
      601 tattgctttt gcaaaattta tttacctgtt ttctggatca acgatagtgt atattatgct
      661 atccttgttt ggattttagg acgtttaaag aggcactttt ttacataact taatccatag
      721 accttttaag catgctgtga gataataata aagaagagtt taaagcgcac cccaaaccac
      781 ttccccataa gtgtgttacg ctttaaactc ttttatattt gaagccatta aaaagcatac
      841 catatttttg aaaaaaagtg agaaaaaagg cttacaattg gagtggtagt taattagtga
      901 cttatttttg attttatagc actgatacta taaaatatag atatcatcat attacacaat
      961 cttaatacta acttaaaaaa tatctccttt cacaagtatg gtgataaaat tcgttccggg
     1021 ctaccttttt aggtagccta ctttaatctt tatacctttc tggatcaacg aaagtatact
     1081 ttatatagtc ataacgccga tgatcgctac gtgcgtccgg cac
//
LOCUS       DAZKCY010000277         1106 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000277 DAZKCY010000000
VERSION     DAZKCY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.289"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..892
                     /locus_tag="ACG8XC_002944"
     CDS             <1..892
                     /locus_tag="ACG8XC_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFP9192533.1"
                     /translation="DVVSLDKQTKDEVKKNVIGNMSSKIGGVIVMGTDNILISTFVNL
                     TAVGIYSNYSLIVTSVQNLCKQITNSITASVGNFAVSNKKKDSFELFKKHFFINHSLI
                     FFSSILLLTLMNPFISLWVGAKYMLPMSTTILIVLNYSVQVYRNTSFVFIESFGLYWS
                     QRKKPIIEAGVNLLLSLLLLIVFDMGINGVLIGTIGSSLGFVIWYEAFIIYKTVFEKR
                     FIHFFLTFWRSFIELIISAIVVFLLNEYLANGGSTFLQFITKTLITIVTTSILYLLLY
                     FRREEFTFIKKILFRYILKK"
     gene            949..>1106
                     /locus_tag="ACG8XC_002945"
     CDS             949..>1106
                     /locus_tag="ACG8XC_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9192534.1"
                     /translation="MGGASIVASKIAKGLIDSGHEVFFVSYLESEKPQVESKYYDLYE
                     KKIFLFKN"
BASE COUNT          381 a          131 c          176 g          418 t
ORIGIN      
        1 agatgtagtt tctctagata agcaaacaaa agatgaagta aagaaaaatg tcataggtaa
       61 tatgtcctca aaaataggcg gggttattgt aatggggaca gataatatat taatttctac
      121 atttgttaat ttaacagctg taggaattta ttctaactat tcattgattg tcacaagtgt
      181 gcaaaattta tgtaagcaaa ttactaattc tatcacggca agtgtaggaa attttgctgt
      241 atcaaataaa aaaaaggatt catttgagct atttaaaaaa cattttttta tcaaccactc
      301 actaatcttt ttttcttcta tattgttatt gactttaatg aatcccttca ttagcttgtg
      361 ggtaggagcc aaatacatgc tacctatgtc aacgactatt ttaattgttt taaattatag
      421 tgtacaggtt tatagaaata catccttcgt ttttatagag tcatttggtt tatattggtc
      481 tcaacggaaa aagcctataa ttgaagcagg tgtaaacttg ttattatctt tgcttttgtt
      541 aatagttttt gatatgggaa tcaatggagt acttataggg actatcggat cgtctctagg
      601 atttgtgatt tggtatgaag catttatcat atataaaacg gtatttgaga aacgttttat
      661 tcatttcttt ttaactttct ggcgaagttt tatagaatta ataatttcgg caatagtcgt
      721 ttttctatta aatgaatatt tagcaaatgg aggcagtacg ttccttcaat ttataactaa
      781 aaccttaata actatagtaa caacatcgat attatatttg ttattatatt ttagaagaga
      841 agaatttaca tttatcaaaa aaattctttt taggtatatt ttaaaaaaat agctttctat
      901 aaaggagtgt tttatttgaa aatagctata ctatctccag ctttctcaat gggaggagct
      961 agtatcgtgg cctcaaaaat tgcaaaagga ttaatagata gtggccatga agtttttttt
     1021 gtttcttact tagaaagtga aaaaccacaa gtagaatcta agtattatga tttatacgaa
     1081 aaaaaaattt ttttatttaa aaattt
//
LOCUS       DAZKCY010000278         1045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000278 DAZKCY010000000
VERSION     DAZKCY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.291"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..942
                     /locus_tag="ACG8XC_002946"
     CDS             127..942
                     /locus_tag="ACG8XC_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9192535.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
BASE COUNT          397 a          159 c          172 g          317 t
ORIGIN      
        1 agaattaaat aaaaaataat tactatctag tacgaaatta attacatagg ataatatatt
       61 aaggtgctgg actacaagta aaaaatactg tatgatcatg acagattcaa gaaaggagca
      121 ataagattgg ttaagatagc aggactttct aaaggtggaa aaaccgtttc acaactttat
      181 aaacaaaact acagagactg gagacagttc agaaaaaaca ttaaggattc ttactttatt
      241 ctaccaacag aacttgaaaa atatttaccg aataacaaag ctattaattt atatctgtat
      301 tactgtttta aggcaaagaa tgaaggcggg gattcctggc attcggtcaa taccatagca
      361 gagtctctag gagtgacaac taaaactata aacacctgga ataaaacctt agttgattta
      421 ggtatgattg cacgtataga cgataaccat ctcagtaaat cgacaatttt acttcctctc
      481 tctagttact ataaattagc ccttgatagt tctttaaagg atgttaccga gagcactgtc
      541 catgaaattg atggcaactt ggttgcagtt tatcatttgt ttgaatggag aaaaaatgag
      601 gctgatgaaa attataacgt tccttataac acactctgtt gtgtttttaa aagaaaaaca
      661 caaaaagata ctatctataa atttatcttg gtccaagatg agaaaataag taatttcgaa
      721 ttaagcactc cttctagtaa gatttatgac gatgcatata gatttgaatg tcaagatcta
      781 gaagccctcc tttccgcaaa taatctaaac aatatagaac atgaaaactt tgttgtaaac
      841 actagattta atttagtttc tgaaaaggac tcagatttgt tagatttgct gatagagctt
      901 actaaaaatc tagataagaa agaaaacatt actgtactat aactagcatc ggtccatcat
      961 taagtttgga ctattagaac acttgctatt acagaaaaag gtatgctagc cacgcttact
     1021 atttattggt atcaaacaag tatct
//
LOCUS       DAZKCY010000279         1037 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000279 DAZKCY010000000
VERSION     DAZKCY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.292"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(131..310)
                     /locus_tag="ACG8XC_002947"
                     /pseudo
     CDS             complement(131..310)
                     /locus_tag="ACG8XC_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          300 a          222 c          131 g          384 t
ORIGIN      
        1 aaaatatcga ccaagatttt tgaaaaagaa atctggtcga tatttttatt taagtgaatg
       61 tttaatttga atcatcgatt gatcgctatc taattgcagc agcatctttt tatgaagaaa
      121 caactttttc ttatctatca acgtagacag gtgttgctgt ttcttctaat agtgtactta
      181 attccttaat attttgtttt ccttcttcta acatccaact actcgctttt tcttcgcctt
      241 cggtaacaaa aggcgccact cccttcgccc atgttgctcg cccacataag acaccattct
      301 ccataaagga caacgcggag ataccaccca tctaaataac atccataaac ctttctagta
      361 gaagaatagt gatagtatcc atcccttttt ctactgggaa tacaattaca cgattcaaag
      421 cttgatacac aacatttccc ttcttataaa ccgctattgt aaccgaattg ttttgaatca
      481 cctttttggg atagaaactg gctcctttcc gcaacttctt aaatgttggt ttcttttata
      541 ctttctttgt agcaactaca aatttcttca aatctctaaa tgtttgcata acaaccgcct
      601 ttctgacttt tactcaggat ggtatttaaa aatcagcaaa ataaggcttc tctccatctt
      661 ttttatttcc tagctgcttt aaagacactc ttgctgtttt ttaatctcta tcaagggggc
      721 tctgaatcta ttttgatgaa cttatcttat taatttctca aatgagctgt cagacacaac
      781 tgtatttttt caatctcata ccggacaact atgccagctt aaaaaaattt cctctggcaa
      841 aacttcttta ttgaaaaatg acaatgaaaa aagaccattc tttttgttga atgctcattg
      901 tgtaaacata tttcctgcga ctgctcttcg aaccattctc agtgcaatcc ctaatttatc
      961 tattaaatct tcttttagtt cctcatcttt catattcaaa atttcctttg tatgttacac
     1021 aagtttgttc tctaaat
//
LOCUS       DAZKCY010000280         1035 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000280 DAZKCY010000000
VERSION     DAZKCY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.293"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1035)
                     /locus_tag="ACG8XC_002948"
     rRNA            complement(<1..>1035)
                     /locus_tag="ACG8XC_002948"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAZKCY010000281          992 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000281 DAZKCY010000000
VERSION     DAZKCY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.294"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>992)
                     /locus_tag="ACG8XC_002949"
     CDS             complement(<1..>992)
                     /locus_tag="ACG8XC_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9192536.1"
                     /translation="LTGNMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKP
                     VLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGAT
                     GVGKSFLATKIFEYAIHEQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAE
                     KDTDGLLATANNGYLFLDEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIF
                     ATTEKGENYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSR
                     EALSLMLSHPFSGNIGKLKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLES"
BASE COUNT          293 a          218 c          167 g          314 t
ORIGIN      
        1 cggattccag catatttaac tgaatcaaga tctcattttt cccaatcgtt aatggttctt
       61 cttgtgttcg actatacaca tccgcacaac tgattttaat gatattcttc agcttaccaa
      121 tattcccact aaagggatga gataacatta aagacaaggc ttctcttgaa acaataatat
      181 ctttttgtaa aacatccgct tcattttgaa aaaataattg aatcagctct aatcgttctc
      241 gaatggggcg atcttcaaaa gttggcaaca taacactaat ttgaatacgc cgatcaaagg
      301 tgtctaataa ataattttca cctttttccg tcgtggcgaa aataaaacga accttagcag
      361 aatgccaatt tacattttca ccgactggac gatagtttcc agtatcaata aatagaaaga
      421 gcttttcttg attttctttc gataggcgat ggacctcatc gagaaaaaga tagccgttgt
      481 tggccgttgc caataaacca tcggtatcct tttcagcccc cgtaaaggcc cccttcttgt
      541 atccaaacag tgttgccgaa agcagttcgg gattgtccgc ataatcggca cagtttaaga
      601 tagcaaatgg tgcatctttt tcaataattt gctcatggat agcgtattca aaaatttttg
      661 tggctaaaaa actttttcca acacccgtgg ctccatttat taatacactg agtccgttag
      721 gcggatattt gacagcggct atacattttt gaatgacctc tctcagactt ccgtcatgac
      781 caactacaga atgaaaggct tcgtttattt gctccgtatt atctacaacc gcatattcat
      841 ctaaaggctt ccacaaaaca ggctttcccg gtaatttctc gaccttttta tcttccacta
      901 atctcgtcaa ataatggctg gcattctgac gagagatacc tagattcttc gaaattttct
      961 cggcagtgac agaagacata ttccctgtta gt
//
LOCUS       DAZKCY010000282          979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000282 DAZKCY010000000
VERSION     DAZKCY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.295"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..590
                     /locus_tag="ACG8XC_002950"
     CDS             <1..590
                     /locus_tag="ACG8XC_002950"
                     /inference="COORDINATES: protein motif:HMM:NF015839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HFP9192537.1"
                     /translation="SEQDRNERTNILMKRLEASTSNFNNKLDVFEEKTKHARLNFETT
                     AKHYIKRLDEDNLKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHN
                     KVVDKSNTALKVMTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHE
                     SAWRYLMLILYAVPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          374 a          136 c          188 g          281 t
ORIGIN      
        1 agagcgagca agacaggaac gagagaacaa acatactcat gaaaagacta gaggcttcga
       61 cctcgaactt taataataag ttagatgttt ttgaagaaaa gactaagcat gcaagattaa
      121 actttgaaac aactgcaaag cactatatta aacgacttga tgaagataat ttgaaattag
      181 attttcaaca agctattcaa gatgaattaa gcgatactaa agacgaaata cgtgaagtga
      241 cgaaacaagc gagagaagaa acaaaagagt ataaagaaat cttagaaagt aaaataaaag
      301 accataacaa agtggttgat aaatcgaata cagcgctaaa agtgatgaca aaaggcgtta
      361 caaatatatt ttttgtgcta attatttttg tacttgtaat gcttgtgaca ggtccgatag
      421 gtcatttctt tggtattgaa catttatata gctttattaa tggttttatt gatgaccacg
      481 aaagtgcatg gcgatatttg atgttgatac tttatgcagt accctatgta ttcttcgcta
      541 tcattctgtg gctcatagca ttgtttttta gagtatttaa tgacctttag gtagatttat
      601 atataaaaag ggctaaatac gcaaaatacc cccttaaaat gcaaaataaa tgcaaataag
      661 ggggtaactc cttatgaaaa aacaactaat agtattataa cacgttttta tgccgagaaa
      721 acttattggt tggaatgggc tatgtgttag ctaacttgtt agcgagttgg ttggacttga
      781 attgggatta atcccaagaa agtaccaact caacaacaca taaagccctg taggttccga
      841 ccaataagga aattggaata aagcaacgaa aggagttgag taaaatgagt gagaaaacga
      901 agaaaatcat tcgagtatca ttagcttttt cacaagtagt agtgcaatca ttgatagttt
      961 attatactta ccagcaata
//
LOCUS       DAZKCY010000283          976 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000283 DAZKCY010000000
VERSION     DAZKCY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.296"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..181
                     /locus_tag="ACG8XC_002951"
     CDS             <1..181
                     /locus_tag="ACG8XC_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9192538.1"
                     /translation="INLYYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRY
                     DYIKYTFVDPERYKD"
     gene            complement(217..588)
                     /locus_tag="ACG8XC_002952"
     CDS             complement(217..588)
                     /locus_tag="ACG8XC_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192539.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(590..>976)
                     /locus_tag="ACG8XC_002953"
     CDS             complement(590..>976)
                     /locus_tag="ACG8XC_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192540.1"
                     /translation="PSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTKNDNQL
                     VITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSELFSSDY
                     DVLDTLDNVLSDYKYMGKSVVATLGE"
BASE COUNT          319 a          165 c          134 g          358 t
ORIGIN      
        1 aattaatctc tattattcta ataagaacta tttcaaaatt cctgctgcga aaactagaat
       61 gaagaagggt gtatactttt tgtttgatat cgtgactgac gtgccggacg cacgtagcga
      121 tcatcggcgt tatgactata taaagtatac tttcgttgat ccagaaagat acaaagatta
      181 aataaaaaaa taacaaacaa actcgcttga aattcattag gtgagtttgt ttattatttt
      241 ttctgcttct cgattcatca gcgccatatc tgttttcgta atggtttcat taggaatata
      301 gtcagcacat tttcttttct ctaacattct atacccacta ttatgtattc ctaaggtatt
      361 caattcctta actgttgaaa acaaattcca aacggtttgg tggcttcctt ttccgctact
      421 tccattataa ataatatcat aattttcttt caaatactca tcagattttt taaaagcact
      481 gtagtatgct cttgaaacag cagttcttct ataagattct ttagaattat atttatcatc
      541 tgattcaatt tgtttggaaa tagataaata ttcattaaag tcaaacattt tattccccca
      601 aagttgctac tacagatttc cccatatatt tatagtcaga taatacatta tcgagagtat
      661 ccaaaacatc gtaatctgag gaaaacaatt cagatgttgt tttgataaca aagaaataca
      721 agtttaaatc ttcatcatat tttatattga tttcagcttt tctttttaaa accgtattta
      781 tcacatcaaa aattttgcca tcattacttg aaattgtgtt aatctcattt tttgtagtaa
      841 ttacaagttg attatcattt ttagttttcg gagtagaact tgcatccaca acaactggag
      901 atgctagaat taccgatgcg caagcaaaaa tcgcagcaaa atttattaag ctaccactcc
      961 gatgtgtatt tgatgg
//
LOCUS       DAZKCY010000284          969 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000284 DAZKCY010000000
VERSION     DAZKCY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.297"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..909
                     /locus_tag="ACG8XC_002954"
     CDS             <1..909
                     /locus_tag="ACG8XC_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP9192541.1"
                     /translation="KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKK
                     ELEKAGLLKQKRGRVNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAK
                     IANTAKPREINDTTVIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTET
                     DQLQNQVLLDNFVDIMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAE
                     ELEQTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKA
                     IAAKLANEQRETFNELPTVSTENWLE"
BASE COUNT          403 a          145 c          166 g          255 t
ORIGIN      
        1 aaagatcgat ttgattattc agtcaaaaat aactgggtag attctgatga taatatattt
       61 ttcatattca cagttgaaga attaatgaaa ttgttacaat gccgcgaagg taaagtatct
      121 aaaatcaaaa aggaattaga aaaagcaggt cttctgaaac agaagagagg aagagtaaat
      181 aaacgtgacg gtaagattga atccatgcct aatttattgt acttaggcaa gccagaagta
      241 acgaatcaag acgtctttaa aatcatggaa gaggaagaca atactgatac caccgtaatt
      301 gcgaaaatcg caaatacggc aaaaccgcga gaaatcaatg ataccaccgt aattgcgaaa
      361 atcgcaaata cggcaaaatc cagtcatatc aaggactcaa ccgtaattgc gaaaatcgca
      421 gacaatctat tctattctaa ttctatagac actaatagac acataataga cactgaaact
      481 gatcagttac aaaatcaagt tttactagat aacttcgtcg acatcatgaa agaggacagt
      541 ataaatacat ttgtaccaga aaatgtatta aacttgataa aaacattttc tagtacttac
      601 gaagacgctc aaaaaaccgt ccaaactatt cataatgcaa agaaaaaagc tgaagaatta
      661 gagcaaacag ttgttgttta tgaagaactg gaatcttatg gaattgatgc tgacaaagga
      721 ttatatatga ctttgctgaa agcttatcaa aaacaaaaaa ctgaaaaagt aaacaatttg
      781 cagaatctaa ttttcgttta tgtaaaaaat tggtttgtcg aaaaagctat tgcggcaaag
      841 cttgcaaacg aacaaagaga aacatttaac gaattaccaa cagttagtac ggaaaattgg
      901 ctagagtagt tgataaaaag cgatcactat aaaaatgatc aattttaaat tagaactttt
      961 ttatgattt
//
LOCUS       DAZKCY010000285          962 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000285 DAZKCY010000000
VERSION     DAZKCY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.298"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..817)
                     /locus_tag="ACG8XC_002955"
     CDS             complement(<1..817)
                     /locus_tag="ACG8XC_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9192542.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLL"
BASE COUNT          323 a          179 c           97 g          363 t
ORIGIN      
        1 ccaacaaaag tctccttgtt ttgtttacaa atatctcatt ttctaatata tagcttaaat
       61 cgccttcaat ttttttaact aattcaatat tacaatcgaa ctcattatct ctaaaattat
      121 gtgtcaatga attattatta ttacatctat aataatagat acaatcttta ataaaacttg
      181 ttatttttga atgttgcaaa tatgaaatat ggaatatgat atcttcagat atataatccc
      241 tttctgacgg aaacttgata ttgtgctgtt gaattatctc agatgaatac agcgcatgcc
      301 aaactgacat tggcaatata aaatcgactt tataactcgg ctcagtgcca atcattccaa
      361 gcaacacttt ttcaattata gcgttatttg taaatatcgt actatcatat cttgctggct
      421 ttgaaataat attattatca tcataatatt cataatatcc agcataacat gtgtctgcat
      481 tactcctttt tataccattt aacattgctt caatataatt agctgcaaca aaatcatcag
      541 aatcaataaa agcaacatat tttcctttta catgttcaag tcctgtattt cttgccaatc
      601 ccaagccagc attattttta tgaataatac gaatccttga atcctttaat gcatagtctt
      661 cacatatttt attagatgaa tccttagatc catcatccac aattataata tctaggttct
      721 tatatgtttg attacacact gattccaaac aacttcctac atacttttca acattatata
      781 caggtatgat tacactaatt agatcactcg aattcatcct tctatccttt cactcacgtt
      841 ttctcaatta gtcaatgacg gcatcaacga ttttcttaaa ttctctgaaa aaaatagact
      901 ctgatcttga tgatgtgaat gacatactat cttcttctag aaaaattatt tcctttaaat
      961 ta
//
LOCUS       DAZKCY010000286          945 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000286 DAZKCY010000000
VERSION     DAZKCY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.300"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>945)
                     /locus_tag="ACG8XC_002956"
     CDS             complement(37..>945)
                     /locus_tag="ACG8XC_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192543.1"
                     /translation="KDLKQYYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFS
                     DIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFEN
                     QFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYIN
                     DLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLS
                     LYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTI
                     QEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          305 a          161 c          145 g          334 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttt
//
LOCUS       DAZKCY010000287          943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000287 DAZKCY010000000
VERSION     DAZKCY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.301"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG8XC_002957"
     rRNA            <1..>943
                     /locus_tag="ACG8XC_002957"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZKCY010000288          912 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000288 DAZKCY010000000
VERSION     DAZKCY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.302"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..670
                     /locus_tag="ACG8XC_002958"
     CDS             <1..670
                     /locus_tag="ACG8XC_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9192544.1"
                     /translation="AGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNNLTILDTAL
                     ENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNLENQLSA
                     KDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINSLNQQI
                     NQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQIVNE
                     HTIQ"
BASE COUNT          405 a          110 c          135 g          262 t
ORIGIN      
        1 tgctggagta attataggat tgatgttagt aattttctcc ggaaagcaat atgctgataa
       61 tattttatta tggggagggg aagataatat tgaaaagatc aacaacaatt taactatatt
      121 agacacagct ctagaaaata aagagcaaaa aattagtatt ttaaattcac aattgtcttc
      181 aaacaaccaa actcttgaac aactaagaac taatattgaa gtttccaaac aaaaagtggc
      241 atctttagaa aacgacaaga atcaattagt atcagagaaa actaatttag aaaatcaatt
      301 gtcagcaaaa gactctgaat tgcaaggaaa acaaaatgaa attaatgcga aaatagacga
      361 aattaaccaa aaagtagctg aaataaatcg attaaatagc gattggaata atcgtttaca
      421 aaatgaaaag aacaaattta atgaagaaca atctactatt aatagtttaa accagcaaat
      481 taatcaaatg ttgagtgaaa aaaataacat tcaaaatcaa ctgcaagcta ctcagcaaaa
      541 gctagaagaa gaaaagtcaa aaaatagtag tttgaataat tacattgaaa agcttcaaaa
      601 agctaaaaat gatgtagagg ataccgcaaa taaagcacaa caaattgtaa atgaacatac
      661 aatccaatag atatagtttt aatttaacaa atggtatgtt tggagataat gagatagaat
      721 ataaatgtca taatattata caatctaaac accgtttcct tttctatttt tgatgacatc
      781 agtatatagc tactcgaaag agtagtcttt ttattattct cgcttattat tgatatgaaa
      841 atttcgattt gacaaaaggt accgaataat tttgtaatat gaattattat caagaagtaa
      901 ttatgatgat aa
//
LOCUS       DAZKCY010000289          906 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000289 DAZKCY010000000
VERSION     DAZKCY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.303"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..767
                     /locus_tag="ACG8XC_002959"
     CDS             <1..767
                     /locus_tag="ACG8XC_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP9192545.1"
                     /translation="IKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKYYISLLCKEE
                     IIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKN
                     KGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGML
                     RNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLE
                     IREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          335 a          144 c          164 g          263 t
ORIGIN      
        1 tcatcaaagt tcctaaattg aagtcattag taagaattaa gcttcataga caaccaaaag
       61 ggattatcaa atctgctaca atatcacgtc attctagcgg taaatactat atttctttgt
      121 tgtgtaaaga agaaattatc gaattaccta aaactaattc cacaattggt attgacttag
      181 gtattatgga ctttgcgatt ctttctgatg gacaaaaaat tgataataat aaattcacgt
      241 ccaaaatgga aaagaaacta aagcgtgaac aacgcaaatt gtctaggcgt gctttattgg
      301 ctaaaaataa aggtaccaat ctatttgaag ctaaaaatta tcaaaaacaa aaacgcaaag
      361 tagctagatt gcatgaaaaa gtaatgaatc aacgtactga ttttctgaat aagttgagta
      421 cagaaatgat caaaaatcac gatattatct gtattgaaga cttaaacaca aaaggtatgt
      481 tgcgaaatca taaattagct aaaagtattt ctgatgtttc ttggtcaaag tttgtgacta
      541 aattacaata caaggctgac tggtatggac gaaaaatcat caaagtagat aaatggtttc
      601 cgtctagtca gatttgttca gagtgcggac ataaagatgg caagaaatct cttgaaattc
      661 gagaatggac ttgtcctgtt tgtcatactc atcatgatcg tgatatcaat gctagtatca
      721 atattctgac cgaaggtcta agactacatt ctatgggatt ggcttagatc caatcatcaa
      781 gaaccgtagg aactacgggg atagcttggt aaataagaga caccgctgtt agtaaagaaa
      841 tacgctatca agtatgctct attcccaaga agctcccact tcaagcgtta agaaccgtca
      901 ggttta
//
LOCUS       DAZKCY010000290          904 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000290 DAZKCY010000000
VERSION     DAZKCY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.304"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..259
                     /locus_tag="ACG8XC_002960"
     CDS             2..259
                     /locus_tag="ACG8XC_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFP9192546.1"
                     /translation="MFDRSRNASIGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLSST
                     LYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(462..827)
                     /locus_tag="ACG8XC_002961"
     CDS             complement(462..827)
                     /locus_tag="ACG8XC_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192547.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          355 a          110 c          160 g          279 t
ORIGIN      
        1 gatttttgat aggagtagaa atgcaagtat aggttttaga actaaacgaa gtctgtcttc
       61 taaaaaaaat tgggtatact cacaaactat tttttatgga ggaattgttt taattagttt
      121 actttcttca acactttatt ctttgaatat tatagatgtt tccacctcaa attcaatatc
      181 tattattgga atcattattg ctgcaattat tacacaactg tttttagtgt ttggagagaa
      241 gaaaagaagt aaaaaataga aaaactagtg atagttttgg tgatagtgat attaaaaatc
      301 agaaaagata gaaaaagcag gagatagata aaatgctgat atttcagcat ttttaagagt
      361 ataaaagaga aaaaaagtaa taaatgtata catgatataa gaaaaacata tatgtataaa
      421 aagaagaaag tagcttctct ttgtacatat atggtttgtt tttatggatg ccgagctttt
      481 ttataagata aggctaccgc ccataacgac ccaaggattt gtggaactaa gtaaatacct
      541 gcaagaagca agatcagctg ggtcggtttt aaaggatgat agaagtaacg ccattcagta
      601 aaattggcgc aaaataaata ggaagtgaca gtagatataa tattggcaat gaataaagat
      661 ataatacgcc tgttcctttg aaaataatat ccagcaaaaa gagataaaaa tatcaatgtg
      721 atgaacccgc ttacggaatg ataacgaaaa tcaagcagta aggcgaaatg cacataagga
      781 acaaaagata aaagcaacgg aaggtttttc cagcaagttt ttatcatctt tcatcaccac
      841 ctaacaagtg ttattatttt gttagtttta ttgtaaattg aatcctgcaa taatcaatta
      901 cttt
//
LOCUS       DAZKCY010000291          881 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000291 DAZKCY010000000
VERSION     DAZKCY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.305"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..>881
                     /locus_tag="ACG8XC_002962"
     CDS             9..>881
                     /locus_tag="ACG8XC_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9192548.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          303 a          153 c          165 g          260 t
ORIGIN      
        1 gagtacaaat gacctatacc catcttacaa cggatgaact tgtaataata gagtcttatt
       61 tcaaaatgaa tcaatctgtt gctaaaactg cccattgctt gaatcgttca agacaaacga
      121 tccataaagt atatctgttc ttcaagcaag gaaaatcagc cttagaatat tatcaacagt
      181 ataagaaaaa caaatcaaac tgtggtagac gtccgcttgt tttacccgag gaacaatcag
      241 aatatattca aagaaaggtt gttcaaggat ggacacccga tgtgattgtt ggtcgtgcag
      301 cgtttcctat tagttgttct gctcgtacca tttatcgtat gttcaaaaag gggctatttg
      361 attcttctga cttaccgatg aaaggcaagc gtaaaccgaa tggacatcaa gaaagacgtg
      421 gaaaacaaac tttccgccgc tctattcatg aacgtgaaaa ggattatagc caattctcaa
      481 atgagtttgg tcaccttgaa ggtgacacta tcgtaggtct gaaacataaa agtgctgtaa
      541 ttaccttagt tgaacgatta tcaaaagtta tcatcacatt gaaaccgtgt ggtagacaag
      601 cgattgatat tgaaaaaaaa ttaaatcaat ggtttgaatc tgtaccgaaa aacctattca
      661 aatccatcac ttttgattgt ggaaaggaat tttcaaattg gaaacagatc agtaatgtca
      721 atgatattgc catttatttc gctgatccag gaacgccgtc tcaaagaggc ctaaacgaga
      781 attctaacgg attgttacgt agagatggtt tattgaaatc tatggatttc aattcagtag
      841 atgaattttt tattcaatct gtcgcatcta aacgaaataa t
//
LOCUS       DAZKCY010000292          854 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000292 DAZKCY010000000
VERSION     DAZKCY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.306"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..820
                     /locus_tag="ACG8XC_002963"
     CDS             119..820
                     /locus_tag="ACG8XC_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9192549.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          309 a          150 c          145 g          250 t
ORIGIN      
        1 aacaaattat atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta
       61 caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat
      121 gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca
      181 tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa
      241 aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg
      301 actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa
      361 tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc
      421 tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc
      481 acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat
      541 acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa
      601 tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc
      661 ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg
      721 acaagtaatc aaacaactac gtgattccgg gctgttagat tatgacaaaa agcgttttac
      781 tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt
      841 gaccctgata aata
//
LOCUS       DAZKCY010000293          842 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000293 DAZKCY010000000
VERSION     DAZKCY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.307"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..237
                     /locus_tag="ACG8XC_002964"
     CDS             154..237
                     /locus_tag="ACG8XC_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFP9192550.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            362..>842
                     /locus_tag="ACG8XC_002965"
     CDS             362..>842
                     /locus_tag="ACG8XC_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HFP9192551.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     "
BASE COUNT          315 a          139 c          127 g          261 t
ORIGIN      
        1 gattcatcgt cattcctcct gctatctttt tctattattc taccttattt gatagtagat
       61 ttaaaacttt gcaacagaac caaaattttg tataatagga attgaagtta aattagatgc
      121 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgtaatgta
      181 gataaaacat ctaccgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      241 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      301 acaaacaaat cgtttaactt ctgtatttat ttatagatgt aatcacttca ggagtgatta
      361 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      421 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      481 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      541 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aacactcgtg tcactttaat
      601 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      661 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc
      721 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
      781 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
      841 at
//
LOCUS       DAZKCY010000294          838 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000294 DAZKCY010000000
VERSION     DAZKCY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.308"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..617
                     /locus_tag="ACG8XC_002966"
     CDS             <1..617
                     /locus_tag="ACG8XC_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFP9192552.1"
                     /translation="NLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLG
                     MDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQ
                     FIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNH
                     TISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            634..>838
                     /locus_tag="ACG8XC_002967"
     CDS             634..>838
                     /locus_tag="ACG8XC_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192553.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESIS"
BASE COUNT          303 a           94 c          156 g          285 t
ORIGIN      
        1 gaaatttaga aaacatggtt tttaatctga gtaatggaat aattccaagt gttagtggag
       61 atacgattcg gtctgaaaaa aattattcta tcattgtttt tgaaaagtta gcacaaacgt
      121 ctataacatt aggaatggat ataatagaag cgtatcaaag tagagatgct ttgatacaag
      181 aaaacgaatt agctgtatcg ttacctgagg tcttaaaagt gagggattct gggattgttt
      241 attatactaa ggaaatcgga aaaactaaga tagagcatct ttctccctta atttcttctg
      301 tcgttcaatt catcggctta aatatctata aaagaattac tgtaaaggaa atagctaatt
      361 atttttctgt aagtgaaact aaattaagag aaagctttaa gaatgaaatg catattacga
      421 tttataatta tatctctaag cgaaaaattt ctacggctaa aataatgttg aaatcaaacc
      481 atactatcag tgaagtttct ttaggtttag gattttcaga ttcttcacac ttttcgagag
      541 tattcaaaaa gtacgcaggt gtatctccta agcaatatca attaggttta gtggacaatt
      601 tcaacaacat tagctgagag gttttatttt attatgagac tatatcattt acgtagtttg
      661 gaggagttga ggaaattgga ttttaacaaa aaattttttt gggaaatttc tgatttgagt
      721 tgtttcgtag aatctgagcg tctaagtaat ggtaataata gtgtgttaaa tgaatatgac
      781 aaagtagtaa ttaataaagt aaaaagttta tgtgaagaag ctagagaatc tatcagtc
//
LOCUS       DAZKCY010000295          805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000295 DAZKCY010000000
VERSION     DAZKCY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.309"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          277 a          135 c          128 g          265 t
ORIGIN      
        1 cgtttgataa atgaggttgg tgttgaacgc ttctctttct atgtagaagc tgcagcagga
       61 taccataaat acgaatacta tattggctat taaagtgcaa taaaattata tttcttttat
      121 gttttacatt ttgtatcaat tctgataaaa ttacctatag gtaattaaaa aatataaaaa
      181 aactagttac aaccttagga cgttgtaact agcttgaagc atactagagc taactaaaat
      241 agcactagaa agcaatgtta aacccgtcca acaaacttga tttaacattg agaccagctt
      301 attcagctaa atatagttga ataagcttct tttgttttaa aaaaacaaaa ggtgtactac
      361 gaataacgca agtaaaagaa atactattac aaccagtagg ctacaagtaa gtattactaa
      421 acattgcgta gttagaacct ttgctctatt gtctaaagcc acaaaattag agatcagctt
      481 tatgatgcta agagcgagct gcatccatgc ggacataggt tcttttgaca ctattaattc
      541 tcctttttag gataagggaa attgctagta gttttttcat tagcaccatc ccccttcatt
      601 tgattgagtc tagtgctgct gtgatagttt gctgtttaat atgcttacat tcttattata
      661 acataaatga aacaaacaca tggttagagg tcgtatcaat caaaatagtg tctcacaaac
      721 gatttattac ttactcctgc tgactaacgg actgttcttc agtaactgta actatatgtt
      781 tgttaaaacc accattacct gtaga
//
LOCUS       DAZKCY010000296          801 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000296 DAZKCY010000000
VERSION     DAZKCY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.310"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..482)
                     /locus_tag="ACG8XC_002968"
     CDS             complement(<1..482)
                     /locus_tag="ACG8XC_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192554.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     "
     gene            448..>801
                     /locus_tag="ACG8XC_002969"
     CDS             448..>801
                     /locus_tag="ACG8XC_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192555.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIK"
BASE COUNT          289 a          104 c           97 g          311 t
ORIGIN      
        1 tttaataacc aaatatatag aacataactt aatattaagt tatttgaaat tccataaaaa
       61 acgactgcag gaattattgt aagaaaagga taaaaatagt tcagtccatc tccataattg
      121 ttaaatgttg tatagttgat tggcccttca aaaatagaag ataacccttt gattcgtgaa
      181 agatgaaaag ataaatcacc tgttgtataa atttcttgag catttctcat aaaatttact
      241 gaatataaag tactaagtaa gataagaact actaaaagaa atgctttttt acttattttc
      301 atagatttgt tgttctcctt ttttaattct attttcagta tactagaatt gtcttttttt
      361 aataagtttt gcgttaaatt tttcatagca ctagtatgat atactagaaa agaatcaatt
      421 tttattactt ttaaatagga gaaaaaaatg aaaaaaaaca aaaaccaaat aatagatatc
      481 atttttatgt tatttctaag ttttgcttac gtcatccctc tattcatgtc taagggaatc
      541 tatcatagtg tcaatcagga tacttacttt catttatctc gaatcatagg actagataat
      601 gtatggagtt ctccagtaaa cttcaataac ttcgatcatc atggaacaat gatgaatatt
      661 ttttatccat ggcttactct ttatccagct tttttatttt ataaaatgat gggaaatctt
      721 gttttaagtt ataatatcta ctattttttt atcacttttt taacaatggt cgtttcttat
      781 ttttctatga aacaaataaa a
//
LOCUS       DAZKCY010000297          798 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000297 DAZKCY010000000
VERSION     DAZKCY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.311"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..307
                     /locus_tag="ACG8XC_002970"
     CDS             134..307
                     /locus_tag="ACG8XC_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192556.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          265 a          129 c          101 g          303 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaat
//
LOCUS       DAZKCY010000298          776 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000298 DAZKCY010000000
VERSION     DAZKCY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.312"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            388..461
                     /locus_tag="ACG8XC_002971"
     tRNA            388..461
                     /locus_tag="ACG8XC_002971"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:422..424,aa:Ile,seq:cat)
     gene            complement(645..717)
                     /locus_tag="ACG8XC_002972"
     tRNA            complement(645..717)
                     /locus_tag="ACG8XC_002972"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(682..684),aa:Lys,seq:ctt)
BASE COUNT          269 a          151 c          125 g          231 t
ORIGIN      
        1 aactaagtac gttatgacat catttgatca ataaatgcaa gacttcaacc aatcaccctt
       61 ggttgaggtc ttttttcatt ttttctgaaa aatctaagca aataatagac taaaaaagaa
      121 ccatgtgaga taataaacat agaaaaaagc ttcagatact ccctcaccct agagtctttc
      181 cccaaaaaga taagtatctg aagctttttt ctttttatga cttggaaata atagcataaa
      241 ataatatatt ttacaaagaa taagtacaat ctagtttttt gctattaaat gtgtaataat
      301 taatgtgcca tcacaacaaa gaatgaaacc cattattatc tagtctatgt ccattctttt
      361 tgtttgcagt agttgtgata gctttccgta cctttagctc agttggttag agcagacggc
      421 tcataaccgt ccggtcgtag gctcgagccc tacagggtac atatatacca aaagcactca
      481 tcaatttaat tggttgagtg cttttttatt gttattaaaa attaaaaaac taaaatacat
      541 acccgaattt catacccgaa aaataaaaaa tgccaccacg tacactcttt tcgaaactac
      601 ctaagtagcc aagaatgtac tggtgacgtg ggtttggcac ccaatgggcc gtgaggggct
      661 cgaacccgcg acccgctgat taagagtcag ctgctctacc aactgagcta acggcccgta
      721 caaaaagtac ttaataatag tagcactaag aattttaaaa tgcaagacta cattta
//
LOCUS       DAZKCY010000299          766 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000299 DAZKCY010000000
VERSION     DAZKCY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.313"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..733
                     /locus_tag="ACG8XC_002973"
     CDS             53..733
                     /locus_tag="ACG8XC_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFP9192557.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGITA"
BASE COUNT          265 a          130 c          160 g          211 t
ORIGIN      
        1 aaaaaatcta ttttagtgta aaattgagaa aaaagacaga gaggacagag taatgaatca
       61 ttttaaaggc aaacaattca aaaaagacgt cattattgtc gctgttggtt actacctgcg
      121 ttacaatcta agctatcgtg aagttcagga attgttatat gatcgtggaa taaatgtttg
      181 tcatactacg atttatcgtt gggtgcaaga gtacagcaaa gtcctctatt atctttggaa
      241 gaagaaaaat agacaatcct tctattcatg gaaaatggac gaaacctata tcaaaattaa
      301 gggacgttgg cattatcttt atcgtgcaat tgatgcggac ggcttaacct tagatatctg
      361 gttacgaaag aaacgggata cgcaagcagc ctatgctttc ttaaaacgac tccataaaca
      421 gtttggtgag ccgaaagcaa ttgtgaccga taaagcacct tctcttggct ccgcctttag
      481 aaagttacag agtgtgggtt tatatactaa gacagagcac cgaactgtga agtatcttaa
      541 caatttaata gaacaagacc atcgacctat taaacgacga aataaatttt atcaaagtct
      601 ccgtacagcc tcttccacga ttaagggcat ggagaccatt cgaggaatat ataaaaagaa
      661 ccgaagaaat ggaacgctct tcggcttttc ggtgtctact gaaatcaagg tattaatggg
      721 aataacagcc taagatattt ggagttcaga gagggcgcgt ttgatt
//
LOCUS       DAZKCY010000300          763 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000300 DAZKCY010000000
VERSION     DAZKCY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.314"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>763
                     /locus_tag="ACG8XC_002974"
     CDS             <1..>763
                     /locus_tag="ACG8XC_002974"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9192558.1"
                     /translation="NRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQEYDK
                     VILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEI
                     AEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKK
                     IEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAI
                     GDCATTFFTSINRSSVDFSISNALRTAYIATQHINN"
BASE COUNT          302 a           90 c          138 g          233 t
ORIGIN      
        1 aatagaatga cactaaattc tgagataaaa aagttatttc tagagacaga agttgacaaa
       61 attgatattc aaaataaaaa gattagttgt gtaaaaaaag atggtacaag attctctcaa
      121 gaatatgaca aagttatatt agctacaggt tcaaagcaaa ttatgctaga tataccgggg
      181 aataatttag ctaatatttt ttcgataaag aattttccaa atgcaaaaga gatttttaaa
      241 gctttggatg atagtactat taaaaaagta ggaattattg gagcaggata tattggagtt
      301 gaaattgctg aagccataag aaaaagaggg aaagaagtat atcttttcga tgttgcagat
      361 agagtgttgt cgacttatta cgaccgtagt ttttcagata aagttgaaga aatactatca
      421 aaaaatggaa tacatctttg tctatcagaa agtcctaaaa aatatttagg aagaaaaaag
      481 attgaaaaaa tagtcactac aagtggtacc caatactcta tggatatggt cgtacaagct
      541 ataggatttc tatctaatag tccaattggg gaaaaggaac tcttgagaga tgttagtgga
      601 gcatatctaa ctaatgaata ccagcaaaca aatataaaag atatatatgc tattggggat
      661 tgtgctacaa ctttttttac gtccattaat agaagtagtg ttgatttttc tatttctaat
      721 gctctaagaa cagcttatat tgcaactcaa catattaaca atc
//
LOCUS       DAZKCY010000301          741 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000301 DAZKCY010000000
VERSION     DAZKCY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.315"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..741
                     /locus_tag="ACG8XC_002975"
                     /pseudo
     CDS             1..741
                     /locus_tag="ACG8XC_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612146.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          288 a          123 c          141 g          189 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataaagatg ccgttctagt aaaaaaagca tttagccgta
      661 tcaaacaacc tctgacagag gttgagattt ttcatactga tcgtggaaaa gagtttgata
      721 accaagctat tgatgaatta t
//
LOCUS       DAZKCY010000302          717 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000302 DAZKCY010000000
VERSION     DAZKCY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.316"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..704
                     /locus_tag="ACG8XC_002976"
     CDS             36..704
                     /locus_tag="ACG8XC_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9192559.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT          190 a          123 c          133 g          271 t
ORIGIN      
        1 gttgctgaaa attttcaaaa gaaaagaggc tagaaatgaa aaaattaaat ttatttacag
       61 aattgtgcgg aatcatttta ggcgcaggaa tctacggatt agctgtgaca ggaatcaata
      121 ttccatctaa gctggcagat ggtggggtta ctggtattgc tttgcttttg cataatcttt
      181 ttggattcgc tccttctgtc acttcactgc tttttaattt acctttattg cttatctcat
      241 tgtttctttt tggaaaacac gcttttctcc ggacgatcgt cggaacattt gcgctagttt
      301 tctttttaca tttatgggag agccttcata tacattttgc tgtagatagt ctactgatca
      361 atagtttgat gacaggtatt ctttctggca ttggttgcgg tctcgtcttt cgattcggcg
      421 gaagcacagg cggaacagat atcatttatc aagccattga aaaatatttt catattaaga
      481 ttggcaaaag tcttttcttg attacttttg gtattttagt tgtctccttg ctttatttgg
      541 atctcacaca ttttctgtat acattactta gctgttctat tctatcttat acattgaata
      601 aagtgaagta tctggaaatt ggatctgttt tgccccaatt ggaagcaaaa cagactacag
      661 aaacatttga accacttgat cctcttgaac agttagagca ctgattctta tttttca
//
LOCUS       DAZKCY010000303          713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000303 DAZKCY010000000
VERSION     DAZKCY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.317"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..417
                     /locus_tag="ACG8XC_002977"
     CDS             <1..417
                     /locus_tag="ACG8XC_002977"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HFP9192560.1"
                     /translation="DLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGN
                     NRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDI
                     VEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(547..>713)
                     /locus_tag="ACG8XC_002978"
     CDS             complement(547..>713)
                     /locus_tag="ACG8XC_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9192561.1"
                     /translation="TNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIY
                     SSRKYINFDK"
BASE COUNT          283 a           63 c          127 g          240 t
ORIGIN      
        1 gatttaactg atatagaaaa agattatata gaaagtttta tggaaagact aaatgcaaca
       61 acagtttttg agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa tcatctattg
      121 ttagatggca ataatagatt aactggaata attgattttg gagattctgg aattatagat
      181 gaatattgtg attttatata cttacttgaa gatagtgaag aagaaatagg aacaaatttt
      241 ggagaagata tattaagaat gtatggaaat atagatattg agaaagcaaa agaatatcaa
      301 gatatagttg aagaatatta tcctattgaa actattgttt atggaattaa aaatattaaa
      361 caggaattta tcgaaaatgg tagaaaagaa atttataaaa ggacttataa agattgatta
      421 tataatatat gaaaagctat tataaaagac attagtatta aatagtttaa aaaaatgaaa
      481 aataataaag gaagtgagtc aagtccagac tcctgtgtaa aatgctatac aatgttttta
      541 ccatttctac ttatcaaaat tgatgtattt tcttgaagaa taaatccatt catcatgtag
      601 gtccataaga acggctccaa ttaagcgatt ggctgatgtt tgattgggga agatgcgaat
      661 aatcttttct cttctgcgta cttcttgatt cagtcgttca attagattgg tac
//
LOCUS       DAZKCY010000304          704 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000304 DAZKCY010000000
VERSION     DAZKCY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.318"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..>704)
                     /locus_tag="ACG8XC_002979"
     CDS             complement(53..>704)
                     /locus_tag="ACG8XC_002979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9192562.1"
                     /translation="SFNYDMLVLMREIFPFNELQWVYQGTINTDVITKLKTLGLPCGL
                     DINENNASLTKDNVNLLHANRLKIGAWTVGYNSFEKMKDLGIDYITTNSPSGYLKYAE
                     LSLLNGFTPTNDNGQVPQFAAELEGGYVFLSFNVKDGTNNNQLTEIAKLPDWAIPHEK
                     QYNQCMIRTSSGVSLATFDTQGWKHSTGVTEGTITIGLGWSSRTTWAAGQCIYKID"
BASE COUNT          220 a          138 c          109 g          237 t
ORIGIN      
        1 cctttcattg caaaataaaa aacaccctct attgagcgtg ttggtagcaa aattaatcta
       61 ttttataaat acattgacct gcggcccagg tagttctcga agaccagcct aagccaatag
      121 taattgtacc ttcagttaca cccgtagaat gtttccatcc ctgcgtatca aaagtagcta
      181 gtgatacacc gctagacgtc cgtatcatac actgattata ttgtttttca tgagggatag
      241 cccaatcagg tagtttggca atttctgtta attgattatt atttgttcca tcttttacat
      301 tgaagcttag aaaaacatat cctccttcta actctgctgc aaactgtggg acttggccat
      361 tatcattagt aggtgtaaaa ccgttaagca aacttaattc ggcatatttt aagtaaccag
      421 atggactatt cgtagtgata taatctattc ctaagtcttt catcttttca aaagaattat
      481 atccaacagt ccacgcgcca attttcaagc gattagcatg tagcaagttt acattatctt
      541 tagttagaga cgcattattt tcatttatat ctaaaccgca aggtaaaccg agcgttttta
      601 attttgtaat aacatctgta ttaattgtac cttgatatac ccactgtaat tcgttaaaag
      661 gaaatatctc tcgcattaaa actaacatat cgtagttaaa actg
//
LOCUS       DAZKCY010000305          697 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000305 DAZKCY010000000
VERSION     DAZKCY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.320"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACG8XC_002980"
     CDS             complement(54..629)
                     /locus_tag="ACG8XC_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFP9192563.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          125 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaacg
//
LOCUS       DAZKCY010000306          697 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000306 DAZKCY010000000
VERSION     DAZKCY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.321"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>697
                     /locus_tag="ACG8XC_002981"
     CDS             <1..>697
                     /locus_tag="ACG8XC_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFP9192564.1"
                     /translation="NRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAGLNN
                     LFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVFTLG
                     LLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVINVS
                     MLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVGELE
                     KETIDDIIKKVKGL"
BASE COUNT          233 a           79 c          120 g          265 t
ORIGIN      
        1 taatcgtaaa caaaatgcta aaaggaatat aattactgga acaattttta agttatatca
       61 gattatcatt ccctttttta tgcgtacact tatgatttat cagatgggaa tggagtatgc
      121 tggattgaac aacctgttca catctatatt tcaaatgtta aatttagcgg aattgggaat
      181 aggtgctgca ctaactgtaa gtatgtataa acctatagca gaagacaata gaaaagagat
      241 ttgtgcttta ctgaacgcat ataaaaagta cttttcaatt attggatgga tagtgtttac
      301 attaggattg ttatgcattc cttttttgag atttttagtt aaaggttcga ttcctggaaa
      361 tttaaatatg acagttttgt atttaatgta tttgataaat actgttttgt cgtattggtt
      421 attttcatat agaaagagtc taatatatgc acatcaaaga aatgatgtaa ttaacgtgtc
      481 tatgctaatt acttttacat tccaatttgt aatgcaggca tatgttctga ttttcttaaa
      541 aaattactat ttgtatttat tggcttctat tttgggacaa atagttctta acttaatgtg
      601 tgctgttgtt tctcaaaaga tgtatcctga ttatactcct gttggtgagt tagagaaaga
      661 aactattgac gatattataa aaaaagtaaa aggatta
//
LOCUS       DAZKCY010000307          694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000307 DAZKCY010000000
VERSION     DAZKCY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.322"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..275
                     /locus_tag="ACG8XC_002982"
     CDS             39..275
                     /locus_tag="ACG8XC_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9192565.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            272..670
                     /locus_tag="ACG8XC_002983"
     CDS             272..670
                     /locus_tag="ACG8XC_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP9192566.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT          250 a          111 c          125 g          208 t
ORIGIN      
        1 ttactataga atgagaatgg tatagagggt ggcgaaatat gaaaaaaatg aaaaagtata
       61 aaattagaaa atctggcaat agcgatgtaa caactatccc accagaagta aaagaattca
      121 tgggtgtgca gacaggtgac gcaatcagtt atgtttttca atctgatggt tctgtacgaa
      181 tgatcaaagc acaagaagaa cctgatattg attcattagt tgattcgatc atgaatcaat
      241 atgaagacgc gttaaaagat ttggttgatt tatgaggtat ctttcgacta aagaaattat
      301 caaaatcaac gcgaaagtta ttttacgtca ctcgccaggc gaaatgatcg gtgtaaaaga
      361 tgcaaatgca ttagatatgg ccgtcaaaca gccttcacaa gcagtattca atcaagaact
      421 ctatcctgaa atctatgaaa aagcggcgat tttagcaatt aatttagcta aaaaacatcc
      481 ttttcataat ggcaataaac gaactgctct tgttgccatg ttgttgtttc ttcaattgaa
      541 taattgtcca gttgcttttt ctcgtcaaga agctgttgat tttatcataa tgattaccac
      601 tagttcattg gatttcgatt cactaaagtc caagataacc agttatttac gacaaaccgc
      661 aataaaatga acgaattaaa aactttattt ctca
//
LOCUS       DAZKCY010000308          692 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000308 DAZKCY010000000
VERSION     DAZKCY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.323"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..>692
                     /locus_tag="ACG8XC_002984"
     CDS             289..>692
                     /locus_tag="ACG8XC_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192567.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEK"
BASE COUNT          264 a           82 c          119 g          227 t
ORIGIN      
        1 tttgagggga agtacatata gttgttgata tagttgctaa tattgttagc taatttgtta
       61 gtaaaactgt atacgaattt agatagtaaa aaaacagttt gttccttata tagaggcact
      121 tttactactg attaaatgtc agatatgctt acaaatatac ttataaatat gcttacaaaa
      181 ttagctaata tttccgttga caaagctttg ttactattgt taaactaaat tgactagcaa
      241 aagtgaaaca taaatttatt aaataaatgt aaaaattagg agaaatcaat gtcttataga
      301 gtacaattaa taatttcaga agatgtagaa agtcagcaat ttggcactaa tgttattaat
      361 aaagtaatta atccagctct ttctattaat gcgcctttaa ttcctactgc tttatcattt
      421 tctgtaacag ctgtggtgtc tgagatagag gatacagaaa agataaaaat agtagaaatt
      481 gaagttttaa acaagaatga aaaacaaatt ttttctacgg gtgaagtatc ggtaagcttg
      541 cctcctcaag ttaacgatat aaactttaat ataaacgcca gaaatgtctt ggttgaagaa
      601 gcaggagagc attatgctgt tgttaaattt aatggaactg agattggaag gcaaatcttt
      661 gatatcaagg tcaacaaacc agtggaaaaa aa
//
LOCUS       DAZKCY010000309          663 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000309 DAZKCY010000000
VERSION     DAZKCY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.324"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..448)
                     /locus_tag="ACG8XC_002985"
     CDS             complement(<1..448)
                     /locus_tag="ACG8XC_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9192568.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          196 a          119 c           90 g          258 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gca
//
LOCUS       DAZKCY010000310          644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000310 DAZKCY010000000
VERSION     DAZKCY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.325"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>644
                     /locus_tag="ACG8XC_002986"
     CDS             <1..>644
                     /locus_tag="ACG8XC_002986"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9192569.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKGDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIP"
BASE COUNT          273 a           56 c          109 g          206 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagga gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
      541 tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat
      601 ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcct
//
LOCUS       DAZKCY010000311          638 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000311 DAZKCY010000000
VERSION     DAZKCY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.326"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..>638
                     /locus_tag="ACG8XC_002987"
     CDS             42..>638
                     /locus_tag="ACG8XC_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9192570.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDE"
BASE COUNT          232 a          118 c           70 g          218 t
ORIGIN      
        1 attataaata gaaagctttt tatataaaaa gagaaaggaa agtggttctt ttgttaacaa
       61 acaaccaatc ctccaatctt gtcaaaaaaa cactaaccta ttccatagat acccatcttc
      121 atttatctgc tgctccctat tttcgactac taataaatga aagcgctaat ttatatgctg
      181 aatttgcaca taaatctata gaactagata atccttccct tttattcatt gataaacagc
      241 aagaagtcca aatcttttct actcaaaagg aaacacaaac ttttatcatt atccagtttt
      301 attttgatga gcaattatta gcacatatta cgggacaatc tttttctata gatcaagaaa
      361 taacatctat cattactttt ctgaaaaatg aacagttcaa aattgattat ctaaaacata
      421 gtaagccagc tatttcctat gatcaggaaa tcaattcttt tttcatacta tgccaacaga
      481 tgagcattca tttgctttcc ttattagtta cttattgtct gcaaaaaatt caattgtctt
      541 ccttaccaag gcattatcaa caggaacttg ctttattgac aataccttca aatgagaaag
      601 tcattcctaa aaaaaagcat attctttatt cagatgaa
//
LOCUS       DAZKCY010000312          637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000312 DAZKCY010000000
VERSION     DAZKCY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.327"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..>637
                     /locus_tag="ACG8XC_002988"
     CDS             51..>637
                     /locus_tag="ACG8XC_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFP9192571.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEA"
BASE COUNT          231 a          106 c          126 g          174 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccat
//
LOCUS       DAZKCY010000313          632 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000313 DAZKCY010000000
VERSION     DAZKCY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.328"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..447
                     /gene="pssE"
                     /locus_tag="ACG8XC_002989"
     CDS             <1..447
                     /gene="pssE"
                     /locus_tag="ACG8XC_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFP9192572.1"
                     /translation="QFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKPLNYSYKAFLNR
                     DEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDDHQLQLIRQFDE
                     MNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
BASE COUNT          242 a           67 c          129 g          194 t
ORIGIN      
        1 caatttgata gattgttaaa agctattgat gatttaattg aaaaaggagt aataatcgaa
       61 agtacatttg cacagagtgg ttatagtgat tataaaccac taaattattc ctataaagca
      121 tttctaaata gggatgagtt tactgaaact atgcaaaaag cagaaatggt gattactcat
      181 ggaggtacag gtgctataat tggggccgta aaaaaaggta aaagggtcat tgctgttcct
      241 cgattggcta agtatggtga gcatgtcgat gatcaccaac tgcagttaat tagacaattt
      301 gatgagatga atattatcta tccttgttat gaattagaaa atttagaaga tgccgttaaa
      361 gaaacaagag ttcggatgtt taacaagtat gtatctaaca cggaaacgat tattaattcg
      421 atagaggtat taatagaaaa aatataaata taaggagtaa agcgttatgc aaagagtact
      481 tatgattggt aatgacagct cggtaaaagg tggaattaca agtataatta cacaaatgct
      541 caattttaat tgggaaaaaa agaatatcga gatgactttt ataccgagtt ataagggtgg
      601 aaatgcaata tttaaatctt tatattttat gt
//
LOCUS       DAZKCY010000314          627 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000314 DAZKCY010000000
VERSION     DAZKCY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.329"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>627
                     /locus_tag="ACG8XC_002990"
     CDS             <1..>627
                     /locus_tag="ACG8XC_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9192573.1"
                     /translation="DMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTRELQYNVI
                     AKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQR
                     LVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIESGIHEK
                     DICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLA"
BASE COUNT          233 a           87 c          108 g          199 t
ORIGIN      
        1 tgatatgatt ttactagtag cgtataaaat tttaactgta aacagtaacg ttgcacgtaa
       61 tattgctagt aatataaggg cgatttatgt ggatgaattt caagatacta gagaattaca
      121 atacaatgta atagctaaat tacttcaaaa caaccctcaa attcagtcaa tgtttgttgg
      181 agatattgac caagcaattt atggatcatt agatggtata gctaaatcag ttgaagaact
      241 agaaatgcta actagtcaca cttttcaatc aaagacgcta catggctgtt acaggtctaa
      301 tcagagatta gtagacttct actctaattt tcagagttgt ccttacgaga ttgaaagcag
      361 agggaataat aaaaacgacc gtggttttat aagttatgat tgcaaagtta ctaaggagga
      421 tttacccaac ataataaaaa atataataaa ggaaaagatt gaaagcggca ttcatgaaaa
      481 agatatttgt gtacttgctc ctcaacacta tcctctgttt tcactgggtg agtatttaaa
      541 gaaagaatta ccgtattgca attttgatgc tataaatata tcaccaatta aggtaaataa
      601 tcatagttta tttttcaagt tagcaat
//
LOCUS       DAZKCY010000315          615 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000315 DAZKCY010000000
VERSION     DAZKCY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.330"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..514
                     /locus_tag="ACG8XC_002991"
     CDS             146..514
                     /locus_tag="ACG8XC_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFP9192574.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          215 a          105 c           92 g          203 t
ORIGIN      
        1 atgtctcttt tctattttat tatttgagac atcttatcat ataaatatag cggttacaac
       61 attacttgtg ttttgttcgt tttttataca taactattgt ttttctggta aaataaatca
      121 aaccacttaa tggaggcgtt ttagaatgga aaaatttcag ccgatcatca atcagatcaa
      181 agaaaaagaa catcaacaaa agatcaaaga cctttttgct aagataacag aaacgtatcc
      241 ccagctagac acaactgtaa aatggaacca acctatgttt actgaccatg gtacctttat
      301 tattgcattt agttttgcta aaaatcattt ttctatcgca ccggaaaaag cagcgattcg
      361 tgcccttgaa aaaaatatcc aagaagcagg ctacgtatat acagataacg tcatcaaagt
      421 tccttggaaa agtgtaatca actgggaatt gattgaacaa ttgatttcat ttaatattga
      481 agataaaaaa ggtcatgata aattttggcg ataaagcttt atacgaataa accttttact
      541 ggcgaggaaa ctattcatca gcttatacag gttttgttca atcctatcct ttttatcatc
      601 cctggttaaa cactt
//
LOCUS       DAZKCY010000316          578 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000316 DAZKCY010000000
VERSION     DAZKCY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.331"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..432
                     /gene="relB"
                     /locus_tag="ACG8XC_002992"
     CDS             109..432
                     /gene="relB"
                     /locus_tag="ACG8XC_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFP9192575.1"
                     /translation="MRFFKKGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
BASE COUNT          204 a           84 c          113 g          177 t
ORIGIN      
        1 tttttaatgt ataataaaga gctcgatcat ttactacaag gatgctagct cctacatttt
       61 cacagttttt tagcaagtaa atttttctaa aatcacatca ggaaatgcat gagattcttt
      121 aagaaaggca attatagggc tatgattacg gttcgtgtaa gtgacgccaa aaaagaatgg
      181 ctttcttata tggctgaatt ttatgggatt actttatctg aacttctaaa aaactattcg
      241 atggaacaac tagaagatga atacaatcgt caaacggctg aaattgccca taaacattgg
      301 ataagaagtg ggaaaacaac agtattcatg gaagaatcct taatgaattt ggtgggctgg
      361 aatgacctgc cgtttaaaat tcacgcccga aattcagaag cagctctgat tggcaaatac
      421 gtgtattggt agagatagaa gataatcaat tcgttgttac cgctattcaa gtagggcacc
      481 gaagaaatgt ctatgattaa gagactgttt atttttacaa aaaaataata actggaatct
      541 agttaaaact cttgaagtga gagatttgac tagatttt
//
LOCUS       DAZKCY010000317          565 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000317 DAZKCY010000000
VERSION     DAZKCY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.333"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..525
                     /locus_tag="ACG8XC_002993"
     CDS             91..525
                     /locus_tag="ACG8XC_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9192576.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
BASE COUNT          198 a           91 c          106 g          170 t
ORIGIN      
        1 cttttattct ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag
       61 tatactatac gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg
      121 agcaatattt acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt
      181 gtgcctttgt ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac
      241 tcagataaaa aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg
      301 aaattacagc ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg
      361 attgaagaag cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca
      421 cagtccacag ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt
      481 gaagatgctg gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa
      541 aaagcaaaaa acagcaagct tttga
//
LOCUS       DAZKCY010000318          562 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000318 DAZKCY010000000
VERSION     DAZKCY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.334"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..466
                     /locus_tag="ACG8XC_002994"
     CDS             125..466
                     /locus_tag="ACG8XC_002994"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192577.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
BASE COUNT          193 a           59 c          101 g          209 t
ORIGIN      
        1 aaataatttt tatggtttat tgaaataagg ttgcgaagga agatgtagag ttatttgcac
       61 tgttgtgttg gtatatctct taatgctgtg tctttttggg gagattaatt agtggagtga
      121 ttatatgagt atagatgatt gtttagaaga acaaataaga ttatttttaa agtttttaaa
      181 agttaaagtt caaaataact acttcacatg ttatagttat gcacaccctg ttttaaatag
      241 tcttcctttt aattctttag tgcaaggatt gattacaaat attgttttat tagtctgtac
      301 accaaatgaa atatttgtaa aaaagatagg cagtggtatt tcttcagtaa gattaaaaga
      361 gaaagaatat actcaaaaga ttattcatat tcctaagcct gaaattaagc gttttgagat
      421 aaaagactat tctgctttag ggctgttctc aattggttac tggtgataaa aaataagaat
      481 actaaaacta tacgtagaat ataagtaatc ggcatcaatc ctaattatgt gattgaatgc
      541 cgatttttgt tacgcgctat tt
//
LOCUS       DAZKCY010000319          549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000319 DAZKCY010000000
VERSION     DAZKCY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.335"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>549
                     /locus_tag="ACG8XC_002995"
     CDS             <1..>549
                     /locus_tag="ACG8XC_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192578.1"
                     /translation="TWQDLNSRYTDWGPEKKLLMTAPSKGSLKLEFELANDEQTALFS
                     DEASFSELIDFVDNLMYTPMYDDEELINERKIIEETVKLYEVIEKNDLSIDFISNDVK
                     LSNISKSSNIKKNLDVLKARVNDKIIENSTLTEDIEVSGEVLAANKSKNYFKIKSLAL
                     GDIGGVFEKELFKKIKNSEKQIT"
BASE COUNT          233 a           50 c           91 g          175 t
ORIGIN      
        1 acttggcaag atttgaatag tagatatacg gattggggtc ctgaaaaaaa attattgatg
       61 accgcaccct caaaaggatc attaaaactt gaatttgaat tagcaaatga tgaacaaaca
      121 gctttgtttt ctgatgaagc atctttctca gaattaatcg attttgtaga taacttaatg
      181 tatacaccga tgtatgatga tgaagaatta ataaatgaaa gaaaaattat tgaagagacc
      241 gtcaaactat atgaagttat tgaaaaaaat gatttatcaa ttgattttat ttctaatgat
      301 gttaagttaa gtaatataag taaatctagt aatattaaaa aaaatctcga tgttttaaaa
      361 gctcgcgtaa atgataaaat aatagaaaat agtactttaa ccgaagatat tgaagtaagt
      421 ggtgaagtat tagcagcaaa taagagtaaa aattatttta agataaaatc attggcttta
      481 ggagatattg gtggtgtttt tgaaaaagaa ttatttaaaa aaataaaaaa ttctgaaaaa
      541 caaattact
//
LOCUS       DAZKCY010000320          543 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000320 DAZKCY010000000
VERSION     DAZKCY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.336"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..361
                     /locus_tag="ACG8XC_002996"
     CDS             167..361
                     /locus_tag="ACG8XC_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192579.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          230 a           68 c           88 g          157 t
ORIGIN      
        1 aagaacaata gccttttatt atatatatta ttcaaattag ttatatcgag tgaaaaaaac
       61 tttcaaattg attcttttta tttatcataa agatatagat acataatcat tttcacttac
      121 atatatttga atattaacta aaaacagcaa gaaagaagag gatcaaatgt attatgtaga
      181 agtaaaaaca aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta acgaatatcc
      241 tttacttggt tcatggaaag aagctgctcc tttttccaaa ccatgtgcca taaaaataaa
      301 gaacgagcta gaaaaagaac tgacttgtgg taaagctgtt gtagatatca tcgaaaagta
      361 aatccaatgt atagaaagga agatcacaca tgaaaagcga cgtacaaatt gctaagatta
      421 tagaattaga agaagaagac gcagtgttga caattgaaaa aaaataaatg tttgtatacc
      481 atatgattat ttcaatatgt atcctggaat tggagatctc gtagagctat atcaagatga
      541 atc
//
LOCUS       DAZKCY010000321          527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000321 DAZKCY010000000
VERSION     DAZKCY010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.337"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>527)
                     /locus_tag="ACG8XC_002997"
     CDS             complement(<1..>527)
                     /locus_tag="ACG8XC_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFP9192580.1"
                     /translation="TKKDLKNYILAIVSSIGSVLILFALFNATKILFQELLPHLKEIN
                     FFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAENTDISNARITIWKSAWEIFKTSW
                     LIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGILGIIPFMAFLIKSFVL
                     NVYRIIYNKCIGKDY"
BASE COUNT          187 a           79 c           69 g          192 t
ORIGIN      
        1 ataatcttta ccaatgcatt tattatagat gattcgataa acattcagca caaaactctt
       61 tattaaaaaa gccatgaaag gaattattcc tagaatacct gtcgatgtta atacattcaa
      121 gtatgcatta tgaatggcaa ttcctacttt cgcaatatat ccatctggaa taattgactg
      181 tgcataagat actacatttt tcggagagac tccgataagc caacttgttt taaatatttc
      241 ccatgcactt ttccatatag taattctagc attagaaata tctgtatttt cagctacgtc
      301 tttcctaaca agagtattat caggtgtttg agtattatta atatgttgac tattattcat
      361 taattcttta tgacgctctg taaaattaaa aaaatttatt tcttttaaat gaggtaataa
      421 ttcttgaaaa agtatcttag ttgcattaaa caaagcgaat aaaatcagaa cagaaccaat
      481 agaagatact atagcaagga tatagttttt taaatctttt tttgttc
//
LOCUS       DAZKCY010000322          517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000322 DAZKCY010000000
VERSION     DAZKCY010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.338"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          169 a           87 c          117 g          144 t
ORIGIN      
        1 tatccataaa actaatataa agcttggtac gattctgttt gtaagacata cgaatagtat
       61 cagaggtagg agccaagctg cctgatatta aattatcgat aaagtagaaa ggttaagcat
      121 tagccgctgc aataactaat gctaggagac agcaacgtgt aaccattacc gcacgtgttt
      181 ctgaatggtg gtagcgaccg atttgaacgg ctagggcgga aatacggtgc aaatgcacgg
      241 tggtttcatg caatgcttac agggcgtatt ttgtaagggt atgtaacgga gataattccg
      301 acggtaatgg atgtgagagg ccaagccgac gctgacggta gggcaaacaa acatagttgc
      361 actctagcga tcgttattta tttttaacgg tcaaactagg gtacaactat gcttaaaaca
      421 cttcgcctca acttctggta gcaaaagtga ttttgaaagt caaaatattt tttatgagtt
      481 atttacaatt ctaaaaaaca agtgtattaa tctacta
//
LOCUS       DAZKCY010000323          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000323 DAZKCY010000000
VERSION     DAZKCY010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.339"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..>514
                     /locus_tag="ACG8XC_002998"
     CDS             179..>514
                     /locus_tag="ACG8XC_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9192581.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKE"
BASE COUNT          188 a           52 c           90 g          184 t
ORIGIN      
        1 attaaaaata ttgttactat gttatcaatc gtataaaaat acttactcgt tatcaagacg
       61 attatttcga atattttagg aaaaataaat tgaatattag agaatgtgat aatacactct
      121 aatatgatca tgtgtaattg gaagcgataa gtgtatactt gatattggag aaataattat
      181 gataatagtg attgcagaaa ttattacaga tatattatat aaattatatg aaccctttgg
      241 gtttgctgtt ttattgacaa ttttaggact ggtctatttt atgtatgcaa cagatggcat
      301 tgctgcaggt gagggctata aacgtatact aatggaactt tatcttcatt taaagaagga
      361 tagaaaatat agaaaagttt gtgctttgat attctatact gcgatgattt tatttacaac
      421 attaatcaat cgaacaattt ggttaaatcc tttatctgat gttatgggag actggtggat
      481 ttataagtat aatacaacaa ctggaacaaa agag
//
LOCUS       DAZKCY010000324          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000324 DAZKCY010000000
VERSION     DAZKCY010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.340"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8XC_002999"
     CDS             complement(<1..132)
                     /locus_tag="ACG8XC_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9192582.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZKCY010000325          513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000325 DAZKCY010000000
VERSION     DAZKCY010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.341"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..502
                     /locus_tag="ACG8XC_003000"
     CDS             32..502
                     /locus_tag="ACG8XC_003000"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFP9192583.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          189 a           93 c           77 g          154 t
ORIGIN      
        1 ttattatata aaatagaaag gagaaaactt tatgtctatt tataattttg aaactacttt
       61 agaagacggc actgtctatt cattgaaaaa atatgcaggc aaaccgataa tcattgttaa
      121 cactgcaaca aaatgcggtc tagcacctca atttaaacaa ttagaagaac tttaccaagc
      181 ttatcagaca caaggtctgg ttatcttagg ctttccttcc aatcaattta aacaagagct
      241 agattcagcg actgaagcag cagaagcttg taaaaccact tatggcgtta ccttccctat
      301 gcatcaattg attgacgtaa atggagaaaa tacagatcct ctttttactt atcttaaaaa
      361 tgaagctcct ggtactgtca ctaaagcaat aaaatggaat tttactaaat ttttgattga
      421 tagaaatggc aaagtaatcg aacgatttgc accacaaact tctccattga aaatgaccgc
      481 tgcaattgaa tccgttcttt aacttaataa aaa
//
LOCUS       DAZKCY010000326          487 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000326 DAZKCY010000000
VERSION     DAZKCY010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.342"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>487)
                     /locus_tag="ACG8XC_003001"
     CDS             complement(<1..>487)
                     /locus_tag="ACG8XC_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9192584.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAZKCY010000327          484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000327 DAZKCY010000000
VERSION     DAZKCY010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.343"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            244..484
                     /locus_tag="ACG8XC_003002"
                     /pseudo
     CDS             244..484
                     /locus_tag="ACG8XC_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          166 a           87 c           69 g          162 t
ORIGIN      
        1 ttattctttt cataaatatt gtaaatagaa gtattaaaaa tatatacaaa aagtattatt
       61 tcgagaaaga taaatatttt caaaaacctt ttcataatcc tcaccctttc ttacactgat
      121 aaatcatgcg tctaatattc aaattatact cgatatgctt tagctaatca gccaactctg
      181 tttgtctact atcattatat caatagacaa tatctgctcc cgtctttatg atcgtgccaa
      241 agcaatcaaa agaaaattgc gaatcactta gatcgaaagt ttactcaaac aaaagcattg
      301 tcagcggttg tcacggattt aacttatgtt cgggtcggga caaattgggc ttatatttgt
      361 ttgatgattg atttattcaa tcgtgaaatt attggccatt cttgcggaaa taaaaaagat
      421 gcgcagcttg taaaaagggc cattcaaagc attccttact cacttcaaga aatcgaactc
      481 ttcc
//
LOCUS       DAZKCY010000328          483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000328 DAZKCY010000000
VERSION     DAZKCY010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.344"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          160 a           95 c           56 g          172 t
ORIGIN      
        1 taaatcactc tacaataacg gcgaaagcac aaaaaataaa aaaattatta cgttattaaa
       61 aaaacagatg aaagatcagg ttttattaga tctcattctt tcatcacttg aagggattta
      121 atgaactact ttttcctctt tgtcgttttt atatattcgt attccttcaa acagtaggat
      181 agcctctttc aatgcttgat atttgaactg tccgttactt aaaaatttag atgtgccgcc
      241 ctcttcctag aacatattct tttttacttt ttcattaatt gacagaccaa tcgaacgaaa
      301 ctgtaaattt cgataggata atgattggtc aaactcattc attttcaaga catctcgatt
      361 catattcaag ataggaatct ttttatcaac atcactttat tcgtattttc ctctaccata
      421 aaagcttcac gctccacaaa attgttttat acactactat tcagcccatt caacgaccaa
      481 tga
//
LOCUS       DAZKCY010000329          476 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000329 DAZKCY010000000
VERSION     DAZKCY010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.346"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>476
                     /locus_tag="ACG8XC_003003"
     CDS             22..>476
                     /locus_tag="ACG8XC_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9192585.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKK"
BASE COUNT          178 a           87 c           95 g          116 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattcaaa agaaatattt ctgaacgtaa aaaaga
//
LOCUS       DAZKCY010000330          473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000330 DAZKCY010000000
VERSION     DAZKCY010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.347"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..413
                     /locus_tag="ACG8XC_003004"
     CDS             <1..413
                     /locus_tag="ACG8XC_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9192586.1"
                     /translation="QVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYLAGQI
                     YTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDIYNQEENQTRHIRNLSDAKLEEEAR
                     KVSNYNKKMAAKYVNEEEFCFSKTKIYKRTRGAS"
BASE COUNT          204 a           58 c           75 g          136 t
ORIGIN      
        1 atcaagtatt ggataagaat caaaataaag ttttagccaa ttcaaaaagt aattttgtaa
       61 agattcattt atttgaaaca tttaaaccaa aagaacacac gtatctggga gaagtatatt
      121 tagcaggaca aatatatact gttaatgaaa aagatagttc gggcaacagc agaaaagtat
      181 ataaatttcc attagcttta ataaatcaag agcaattgat tgaagataaa gatatatata
      241 accaggaaga aaatcaaact agacatattc gtaatttatc tgatgctaaa ttagaggaag
      301 aagcaagaaa agttagtaat tataataaaa aaatggctgc aaaatatgtt aacgaagagg
      361 agttttgctt ctcaaaaaca aaaatatata aacgtacccg tggtgctagt taattttaat
      421 atcctaaaga atatctcaaa gttccttcaa aacgtcgttg tgctcgactt aat
//
LOCUS       DAZKCY010000331          471 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000331 DAZKCY010000000
VERSION     DAZKCY010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.348"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..231
                     /locus_tag="ACG8XC_003005"
     CDS             <1..231
                     /locus_tag="ACG8XC_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9192587.1"
                     /translation="LFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQS
                     YLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            241..>471
                     /locus_tag="ACG8XC_003006"
     CDS             241..>471
                     /locus_tag="ACG8XC_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9192588.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNH"
BASE COUNT          191 a           45 c           78 g          157 t
ORIGIN      
        1 ttatttttag gaaatgtatt actaaaagta attgtagatg gattcattaa ctcagaggat
       61 tcaatgattt ccggtggaag tttaataaat aatagtagtt ttatgtcaaa tataacaaca
      121 cttggacaaa gttatttaat attaataagt attattgttt tatcagttgt atttgcttct
      181 tcattaatgt taatcaagaa gcctaaagaa atcttatcaa aaataagtta ggagaaaata
      241 atggatatat tagaaataaa gaatgtagca tatagttatg caaattctaa agaaaaagtt
      301 ttgtcaggag taaatcaaaa atttgaactt gggaagtttt atgcgatagt aggaaagtca
      361 ggaacaggaa aatctacact tctttcctta cttgctggac ttgataaacc tcaaacagga
      421 aaaatattgt ttaagaatga agatatacaa aataaaggat atagtaatca t
//
LOCUS       DAZKCY010000332          455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000332 DAZKCY010000000
VERSION     DAZKCY010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.349"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..300
                     /locus_tag="ACG8XC_003007"
     CDS             103..300
                     /locus_tag="ACG8XC_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFP9192589.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            302..>455
                     /locus_tag="ACG8XC_003008"
     CDS             302..>455
                     /locus_tag="ACG8XC_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9192590.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLI"
BASE COUNT          174 a           46 c           84 g          151 t
ORIGIN      
        1 ttcaggaatt attttgctac gtttcaagaa ggaattgttt tttgaaattt aatgaatgtt
       61 agaataatag tctaaaaaca ttaatttagg ggtgattaga ttatgaaaca tttaaaaatt
      121 ttgtctatta aagagacaca acttatctat gggggtacca ctcatagtgg aaaatattat
      181 ggaaatggag tgtattgcac taaaaataaa tgtacggtcg attgggccaa ggcaactact
      241 tgtattgcag gaatgtctat aggtggtttt ttaggtggag caattccagg gaagtgctaa
      301 aatgaaaaaa aatgctaagc aaattgttca tgaattatat aatgatatat ctataagtaa
      361 agatcctaaa tattctgata ttcttgaggt tttacaaaag gtatatttaa aattagaaaa
      421 acaaaaatat gaattagatc ccagtccttt aataa
//
LOCUS       DAZKCY010000333          437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000333 DAZKCY010000000
VERSION     DAZKCY010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.350"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          134 a           67 c           80 g          156 t
ORIGIN      
        1 ttatgaaaac aatcacaaac gtaggtttta aagtcataat cgttagatat tttagttatt
       61 tttcattcaa aaaaataggg aactagtgtt tgcatatgtg cttttttgag ttataataat
      121 tcatgaagag aggtaattca cttatttccc caagctgaaa agcctgatat tacttctttt
      181 caggcagtgc tgctaatatt caattacact tttttgtata tttttagttg ttttaaattt
      241 aattgtctga tatgcttgtg atgaattgat tttcgttctg cacttttgga cgataaattg
      301 tgataattga aaatacaaaa aaaagaaagg cccctgtaag gctcgggaga ctggtaatct
      361 ccgtcagtgt cctacaggaa agcattaaga tgacgcctta atgcgtgccc ttcagtgtta
      421 cggtattacc cgcaacg
//
LOCUS       DAZKCY010000334          426 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000334 DAZKCY010000000
VERSION     DAZKCY010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.352"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..350)
                     /locus_tag="ACG8XC_003009"
     CDS             complement(<1..350)
                     /locus_tag="ACG8XC_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9192591.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNN"
BASE COUNT          118 a           71 c           70 g          167 t
ORIGIN      
        1 tgattattcg ttgtataact ctgaacagga tttttcttac ttttgaaacg tggaaatcca
       61 acggatttat cccgaaaaaa attcttgtat gctttatcta aattaagttg ggcattggct
      121 aaagcaaggc tatcaatttc tttcaaaaac ggaaattctt tcttgtattt agctggtgtc
      181 ggaaaagtca tttttttaga agaatcattt ttaacttctt catacgcctt ttttcggtca
      241 tcaagcatta gattgtagac cttacggaca caaccaaaag atttggcaaa gaagatttct
      301 tgttcctctg ttggatagat tctgaatttg tatgctttta gttgttccat aaagttcacc
      361 tactttctat ttatgattgt ttccttgatt ttggatatac ttcttaatca catcaattgg
      421 tgcgcc
//
LOCUS       DAZKCY010000335          422 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000335 DAZKCY010000000
VERSION     DAZKCY010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.353"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>422
                     /locus_tag="ACG8XC_003010"
     CDS             <1..>422
                     /locus_tag="ACG8XC_003010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9192592.1"
                     /translation="IYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNL
                     PVGWAIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTS
                     RSEGLPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGI"
BASE COUNT          152 a           43 c           87 g          140 t
ORIGIN      
        1 atttataacc cattgacatt aagcggctct agtttaataa gtaaccttga taaaaaaatt
       61 atttcattta ctgccagata cgatataaat attaaagggt ttgattattt atgtgaagta
      121 gctaaaaact tacctgttgg atgggctata aggattgctg gagatggtac agaagaagaa
      181 cgtaaagagc tgaaaagatt aattgagaaa aatggtgtgt tagataaaat tatttgcgca
      241 ggtgcattat ttggagatga attaatcaat cattatatag aaagttctat tttcatatct
      301 acttctagaa gtgagggact accattagtg ttgatagaag caatgactta tggattacct
      361 gtaataagtt ttgatcatgg tggtgctaag gaaatattac aagatggaaa ttatggaatt
      421 tt
//
LOCUS       DAZKCY010000336          416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000336 DAZKCY010000000
VERSION     DAZKCY010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.354"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>416)
                     /locus_tag="ACG8XC_003011"
     CDS             complement(<1..>416)
                     /locus_tag="ACG8XC_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9192593.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFT"
BASE COUNT          121 a           73 c           86 g          136 t
ORIGIN      
        1 tgtgaaatct tccgtcatga gtgaaatgag ggttgcacta aaaagactcg tcgattcgtc
       61 tcgcatttct cgagcaagcg tattacattt gtacgttaga ttttcaagaa aatgttgctt
      121 tgtacgaatc aattgcttaa taagttgata gcgagtacgt gttaaacgct gtaaggccat
      181 gtatttctct tctttgatag gggaagttgt gaatcgttga atacgcaaga aatcagcgat
      241 tcttaatgca tcgtttcgat cagttttatt ctcatcaaac atgcgactaa attgtttcac
      301 ccgaaaagga ttttcaatcg ttacaagtgt gttgagcttc tttagtttct catcttcatg
      361 aaaaaacatg gaaggatgga agctgtacat ggacgttgac tccatgccaa tcacaa
//
LOCUS       DAZKCY010000337          412 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000337 DAZKCY010000000
VERSION     DAZKCY010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.355"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..186)
                     /locus_tag="ACG8XC_003012"
     CDS             complement(<1..186)
                     /locus_tag="ACG8XC_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192594.1"
                     /translation="MEDKKEKLFDLIANAYNSKSYNNVEVKEGFLQDAKKIREDKPYP
                     EIIISVYKHLSKLKAEER"
     gene            complement(176..379)
                     /locus_tag="ACG8XC_003013"
     CDS             complement(176..379)
                     /locus_tag="ACG8XC_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFP9192595.1"
                     /translation="MILGIVLLSVSTLGITVDAATYYGNGVYCNTQKCWVDWSRARSE
                     IVDRGVKAYVNGFTKVLGGVGGR"
BASE COUNT          135 a           85 c           39 g          153 t
ORIGIN      
        1 gcgttcctca gcttttaatt tagataaatg tttgtacact gatataataa tttcaggata
       61 tggtttatcc tctcgtattt tcttagcatc ttgaagaaat ccttctttta cttctacatt
      121 attatatgac tttgaattat atgcattcgc aattaagtca aaaagttttt cttttttatc
      181 ttccaccgac tccacctagc acttttgtaa aaccattaac gtaagctttt actcctctat
      241 caacaatctc agagcgagct ctactccaat ctacccaaca tttttgagta ttacaataaa
      301 caccatttcc ataataagtt gcagcatcta ctgtaattcc taaagtagat acactcaata
      361 aaacaattcc taaaatcaaa aaatactaac aaattttttc ttcatataat tg
//
LOCUS       DAZKCY010000338          397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000338 DAZKCY010000000
VERSION     DAZKCY010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.356"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>397
                     /locus_tag="ACG8XC_003014"
     CDS             <1..>397
                     /locus_tag="ACG8XC_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFP9192596.1"
                     /translation="DYEISYNEVVTITPRNYKLNFRKVNDLDTHYAMIGIAAGIADYP
                     LYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVGEAKKLLK
                     NINLANINYSDELPLSPLHWLLADKEKSIV"
BASE COUNT          143 a           54 c           70 g          130 t
ORIGIN      
        1 tgattatgaa atatcttaca atgaagtagt cactattact ccaagaaatt ataagttgaa
       61 ttttcgaaag gtaaatgatt tggatactca ttatgcaatg attggtattg ccgctggtat
      121 agctgactac cctctttatt acgatgcgac aaatgaaaaa ggattgagta tggctggact
      181 aaatttttct gggtatgctg attataaaga aatacaagaa gggaaagaca atgtatctcc
      241 ttttgaattt attccttgga ttttaggaca atgctcaaca gtaggagaag ctaaaaaatt
      301 gttaaaaaat atcaatttag caaatataaa ttatagtgac gaacttcctt tatccccttt
      361 acattggcta ttagctgata aagaaaaatc aattgtc
//
LOCUS       DAZKCY010000339          387 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000339 DAZKCY010000000
VERSION     DAZKCY010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.357"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..345
                     /locus_tag="ACG8XC_003015"
                     /pseudo
     CDS             64..345
                     /locus_tag="ACG8XC_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT          136 a           59 c           69 g          123 t
ORIGIN      
        1 tttgttgatt taccaacatg tttagacatt ttcctctagg aaaaaattaa ctagcaccac
       61 gggtataaac gtaatccctt cgtatctgaa tatgttaaac gacttgcaaa aggtatttgt
      121 caactttgcc aagaaaaagg gccatttata aaagacggtg taccttattt acattgtcat
      181 catatagaat atttgtcgca aggtggaaaa gatgtgattg aaaactgtat cgcgttatgt
      241 cccaattgtc atgctagaat tcatgaactt gagttacctg acgataaaga aaaattgctg
      301 aagatcgttg aaaaaagaga aaatagcttt tctaaaatac cgtaatttat ttttatagga
      361 caaataagct aatgtgagct atgcctt
//
LOCUS       DAZKCY010000340          370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000340 DAZKCY010000000
VERSION     DAZKCY010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.358"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..>370)
                     /locus_tag="ACG8XC_003016"
     CDS             complement(44..>370)
                     /locus_tag="ACG8XC_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9192597.1"
                     /translation="GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVE
                     FVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSD
                     TSGEAA"
BASE COUNT          102 a           91 c           66 g          111 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggtt tggttatcaa
      361 attcctttcc
//
LOCUS       DAZKCY010000341          360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000341 DAZKCY010000000
VERSION     DAZKCY010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.360"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>360
                     /locus_tag="ACG8XC_003017"
     CDS             <1..>360
                     /locus_tag="ACG8XC_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9192598.1"
                     /translation="GKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIV
                     GIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKE
                     FSNWKAISNQHDIDIYFA"
BASE COUNT          120 a           69 c           63 g          108 t
ORIGIN      
        1 ggtaaaagaa aacccaatgg ccatcaggaa aaacggggaa aacaacagta tcagcgctca
       61 atccatgatc gacctgataa ttatcctgat ttcaattctg agtttggtca ccttgaaggt
      121 gatacgatcg ttggcattca tcataaaagt gccgtcatta ctttagttga aagattatct
      181 aaagtcatta tcacgattaa acccaacggc cgtaaggcat tagatattga aactgccctt
      241 aatcaatggt tttctcgctt ccctaaaaac ttctttaaat ctattacgtt tgactgtgga
      301 aaagaatttt ctaactggaa agccattagt aaccaacatg atattgatat atattttgcg
//
LOCUS       DAZKCY010000342          353 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000342 DAZKCY010000000
VERSION     DAZKCY010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.361"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..>353
                     /locus_tag="ACG8XC_003018"
     CDS             73..>353
                     /locus_tag="ACG8XC_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9192599.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRY"
BASE COUNT          126 a           41 c           71 g          115 t
ORIGIN      
        1 ataataacaa ccaacctatt tacgagttgg ttgttaaagg ttattattta tttgtaaggg
       61 gggaagaaag gaatggatga aaaaacggaa ttaacagtca aatatcaaaa tgaattgaat
      121 ttagtaccgc tgaaaaattt caatgctaag cagatggatt tattttttgc attatgcgct
      181 cgtatgaaag atcgtggttt gaaaaaagtt agattttctt tcgaggaatt aaaagagtta
      241 agtgattata agatgactgc tacaaaagcc tttgtcggtg atttagaaaa gctctataaa
      301 gatatgctta atcttactta ccgtactgaa gatgaggatt ctattcgcta ctt
//
LOCUS       DAZKCY010000343          343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000343 DAZKCY010000000
VERSION     DAZKCY010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.363"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..>343
                     /locus_tag="ACG8XC_003019"
     CDS             44..>343
                     /locus_tag="ACG8XC_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFP9192600.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
BASE COUNT          123 a           37 c           63 g          120 t
ORIGIN      
        1 gctatgttat actctattag gaaatataag aaggagtata aaaatgaaca aacataatag
       61 aaaaataacg aataacaaag aactgatctt gttactaata gggatttggg gtatcttttt
      121 actctttaat atgtatatgc cgactatgag agcggatgac gtagtttatg caagtagatt
      181 agatgaactt ggctatttgg gtgcttcgat agaacattat aagacatgga gttcaagaat
      241 tattatagaa ctattcttaa tgttcttttc taaacacttt atgctatgga aattattaaa
      301 ttctacgatt atgttaggaa ttgtagtatt actttgcaag tat
//
LOCUS       DAZKCY010000344          341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000344 DAZKCY010000000
VERSION     DAZKCY010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.364"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>341
                     /locus_tag="ACG8XC_003020"
     CDS             <1..>341
                     /locus_tag="ACG8XC_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFP9192601.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAZKCY010000345          339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000345 DAZKCY010000000
VERSION     DAZKCY010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.365"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..157
                     /locus_tag="ACG8XC_003021"
     CDS             <1..157
                     /locus_tag="ACG8XC_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9192602.1"
                     /translation="RKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQM
                     LSQPMEN"
BASE COUNT          128 a           62 c           58 g           91 t
ORIGIN      
        1 ccgcaaggaa cagttccaaa atgaagagtc gatggaacgt ttcttagtct cgtcttttga
       61 tacttacaac caaaaattcc taggtcgcag tcataaaggt ttccaacagg ccgaaggcga
      121 gctcgaacaa atgctgagtc aaccgatgga gaattagaaa caatctacag tagggaagaa
      181 ccattgacac aaaattattg acgctcccaa ctaaaagtgt caaaataatg atgaaatcat
      241 ttagttctta taaattagaa aagagaaaat aaatattcca tatctacttt attggatccg
      301 taacatgact aaattctgaa tactttttaa agcttatca
//
LOCUS       DAZKCY010000346          327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000346 DAZKCY010000000
VERSION     DAZKCY010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.366"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..278)
                     /locus_tag="ACG8XC_003022"
     CDS             complement(132..278)
                     /locus_tag="ACG8XC_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFP9192603.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT          111 a           57 c           38 g          121 t
ORIGIN      
        1 agatcagtat tagtcatata aaataagaga aaaaagaaaa agtttcccac gaacattctt
       61 aatagataag cttttgtatc gatagtaatc aagagatccc ctcctgattt taggatatca
      121 taaaaaaaat tttagttaca ctttttaaat aatgaaaaaa cacattccac taaatttgat
      181 gccggttttc cttgaggttt tgttccagca cctcctttaa tttttttaag ctcttgatct
      241 gaacattgtt tagcatttag tctatttttt tcttccatta cgattccccc tacaaactat
      301 aaatactttt atgtcactga ttcaata
//
LOCUS       DAZKCY010000347          323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000347 DAZKCY010000000
VERSION     DAZKCY010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.367"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8XC_003023"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8XC_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFP9192604.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAZKCY010000348          317 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000348 DAZKCY010000000
VERSION     DAZKCY010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.368"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>317
                     /locus_tag="ACG8XC_003024"
     CDS             <1..>317
                     /locus_tag="ACG8XC_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFP9192605.1"
                     /translation="RRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQ
                     EQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYS
                     LTDV"
BASE COUNT           96 a           60 c           74 g           87 t
ORIGIN      
        1 cggcgccgaa tcggtcgaat catgaaacgc cgcggattga catctaccta tacgatcgct
       61 cattttaaag ggcaacgaac agcttgtaat gaagcgaaaa cagcgaatgt attagatcgg
      121 acctttacac aagaacagcc attggaagcc atcgttacgg atcttactta tgttcgcgtg
      181 gggaaaaagt ggcattatat ctgcttaata cttgatttgt ttaatcgaga aattattggt
      241 tattcctgtg gtgagaagaa agatgcctca ttggtaaaag aagcctttgg acggataccg
      301 tattctttaa cagacgt
//
LOCUS       DAZKCY010000349          297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000349 DAZKCY010000000
VERSION     DAZKCY010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.372"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          125 a           45 c           41 g           86 t
ORIGIN      
        1 gatatttctc ctttattaaa tctgaagtac ttatattaaa ataattctaa actatttacc
       61 tcaaaaagtg caataaatta tttctgaaca ataaaatgat agtcaaaaca aaaaaatatt
      121 gttaatcgtt cagagccatc cgagagtgta aaatattttg tgtaaatgaa aaaatccata
      181 caaaaaagga aggcgcttct gtagaataaa gttaacgaca accaattcac agaaaagagg
      241 acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact aaaggcg
//
LOCUS       DAZKCY010000350          291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000350 DAZKCY010000000
VERSION     DAZKCY010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.374"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>291
                     /locus_tag="ACG8XC_003025"
     CDS             <1..>291
                     /locus_tag="ACG8XC_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9192606.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLL"
BASE COUNT           97 a           62 c           52 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct c
//
LOCUS       DAZKCY010000351          282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000351 DAZKCY010000000
VERSION     DAZKCY010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.denovo.377"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACG8XC_003026"
     CDS             <1..>282
                     /locus_tag="ACG8XC_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFP9192607.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAZKCY010000352        13646 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.guided.068, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000352 DAZKCY010000000
VERSION     DAZKCY010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.guided.068"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..272
                     /locus_tag="ACG8XC_003027"
     CDS             <1..272
                     /locus_tag="ACG8XC_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP9192608.1"
                     /translation="TFESMYFQKKLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFL
                     HTPFQPIPSELLEFFDAENWAKRFFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(415..1356)
                     /locus_tag="ACG8XC_003028"
     CDS             complement(415..1356)
                     /locus_tag="ACG8XC_003028"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9192609.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1482..2603
                     /locus_tag="ACG8XC_003029"
     CDS             1482..2603
                     /locus_tag="ACG8XC_003029"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9192610.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            2861..4363
                     /gene="eat(A)"
                     /locus_tag="ACG8XC_003030"
     CDS             2861..4363
                     /gene="eat(A)"
                     /locus_tag="ACG8XC_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP9192611.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            4430..4957
                     /locus_tag="ACG8XC_003031"
     CDS             4430..4957
                     /locus_tag="ACG8XC_003031"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9192612.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            5169..6230
                     /locus_tag="ACG8XC_003032"
     CDS             5169..6230
                     /locus_tag="ACG8XC_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP9192613.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6233..7129
                     /gene="murQ"
                     /locus_tag="ACG8XC_003033"
     CDS             6233..7129
                     /gene="murQ"
                     /locus_tag="ACG8XC_003033"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9192614.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            7143..9125
                     /locus_tag="ACG8XC_003034"
     CDS             7143..9125
                     /locus_tag="ACG8XC_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFP9192615.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9195..9641)
                     /gene="nrdI"
                     /locus_tag="ACG8XC_003035"
     CDS             complement(9195..9641)
                     /gene="nrdI"
                     /locus_tag="ACG8XC_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9192616.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            9852..11276
                     /locus_tag="ACG8XC_003036"
     CDS             9852..11276
                     /locus_tag="ACG8XC_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFP9192617.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(11453..12226)
                     /locus_tag="ACG8XC_003037"
     CDS             complement(11453..12226)
                     /locus_tag="ACG8XC_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9192618.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(12223..13128)
                     /locus_tag="ACG8XC_003038"
     CDS             complement(12223..13128)
                     /locus_tag="ACG8XC_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9192619.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13289..13609)
                     /locus_tag="ACG8XC_003039"
     CDS             complement(13289..13609)
                     /locus_tag="ACG8XC_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFP9192620.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
BASE COUNT         4316 a         2463 c         2915 g         3952 t
ORIGIN      
        1 gaacgtttga atctatgtat tttcaaaaga aactagtgac agataatcaa gccatagctc
       61 attatcagtt ttatcattct gaaaatattt ttttgcttca agaacgtgat ttagatgaat
      121 taccggaatt tttgcatacg ccatttcaac caatcccatc agaactgttg gaattttttg
      181 atgcagaaaa ttgggcaaaa cgttttttca ctactgactc cgctatttat gaacaatatg
      241 aatacgatct ttcctctgtt aaaaataaat aaaagtgcca accaatcata gatgaaaata
      301 aacaagcgca attcttatgg caacatgaga attgtgtttt tctttttgaa ctttcatggg
      361 ggtaaaaaaa tgagaaccag ctgctgatcc agctgattct catcagagag ccttttattc
      421 tgcgccaagg attccttctt ttgcatggac taattgggtc aaaaaggcac aatatggtgt
      481 agcaatatca tatttttgtc cttttcgcca aacggcacca ttgatgtagt cgatttcagt
      541 caaacgatgg tttttgatca ggtcttggta catagatggg taatgcaacc cgatgttgtc
      601 aggatcataa gtagaagcaa tatgttggta cacttcttct tgatctaatg tgattccttc
      661 ttttgctgcg atagaagcaa actcagatac aatcgttcga accatgccct ctgccgcttt
      721 ttgcgcacct aattcagcaa tattacaatc caatatcgtg cataatccat ttaatgttcc
      781 gtttacacaa gcttttcgcc aaatggaata acgaacatta tgactataga ttggattcaa
      841 ttttgcttct tgaaagactt ctacaacttt ttttgtaaat gcttcgcctt ctggttccaa
      901 gttctctagt tcgatttctc catcacctaa taatgtgact tttccgggac cagctagacc
      961 agctgtccac atcgtgatcc caaataagat atttttcttt ggtacatatt tttccagtac
     1021 atcttcatga cctaatccat tcagtaaaca caaaacataa gtattctcag taataatgga
     1081 ttggatcgaa cgaaacatat catctaattg attcgctttt gtcaaggcta cgatcaaatc
     1141 cacgtgtttg ttgctttcga tgatttcttc tggtgaatag attggcagtt ttgcaactac
     1201 ttctttgcca ttaaagtcag caatcaatcc atttgtacga atggcttcaa tgtgagcagg
     1261 ccagcgatcg atcaagacga cctcatttcc gctttgatgc aacattaatc cgattcggct
     1321 gcccattgct ccggctccag ctattgcgat tttcatgttt tttctctcct ttattcaatg
     1381 agttcccttt tagtatatag aaaaacagaa gaaaatagca ttgttttgtt tgaaaaaagt
     1441 gaaaaaaagt gtcttactta agaagaaagt gagggatata gatggatatt ttggcggcaa
     1501 tcgattcatt taaaggaagt gcttcttcat ttgaattaaa tcaggcagtt ctaaatggtt
     1561 gggaagaccg taaaggaaga aaaatcaatg tgccgattgc agatggcgga gaaaatactg
     1621 cagaagcaat ctattacgcg ctcgggggaa cctggcgtac gattgatggg atggatctct
     1681 tatttcgtca gcgaaaaatc agttatttat tgacgaactg gcaaggaaaa aataccgcag
     1741 tcattgaatc tgcacaagta ttaggattag atttacttga taaaccaacg gatcaaacca
     1801 tccgacttgc ttctagttat gccttgggag agcttatcaa agatacactg cttcaagaag
     1861 tggaacaaat cattgtcact ttaggaggaa gcggatgtag cgatggtggt ctaggattac
     1921 tccaatcttt gggtgcttct ttagaagggt acacagaagg aaatcctttg ttatctacta
     1981 gaaaacttca tttggatcga aggaacgttc ttgtttctaa agtaaaagtg atcatcgctg
     2041 cagacgtgac caacccatat acaggtcaac aaggagctgc ttggcaattt ggaagacaaa
     2101 aaggaggaac ggatacgacg ttagctttct tggataaaca agctgaaaag attgctcagc
     2161 agataaaaga tgtttgggag gtggatttag atgacatccc aggaagcgga gcagcaggcg
     2221 gtattggcgg agcccttttt ttgttaggag cgaagatggt ctctggtttt gagttggtca
     2281 gccaaatcac tggattagaa aaacagatcg aacaagcgga tgtcatcatt acaggagaag
     2341 ggcggattga tcagcaaaca atccacggaa aagttccata tggcgtagca gtgcttgcaa
     2401 aaaaatatcg aaaaccaata atcgctattt gcggaagtag agaccgagag ctggaaggac
     2461 tctttgattt tctccccatt gtcttaagca ttcaattagg accaatcgat ttagaggaag
     2521 caatggaaca caggcaaaca ttagaaaaaa cagaaatctt aggacagaca ctcagcagat
     2581 ttttcaacat tctcccgaag tagttgacaa aaacaatttc tttatgctat cgtaatttca
     2641 aatataggac aaagtgaggg atccgtgcat tgaaaaatta agtcgattga tgaaagctgc
     2701 attcagactg taccttgtgg agacaaggga gcagtgtgca gattttcggg attggcttgg
     2761 ttctttcgat gtatgggtct ttttgtgtgc cccaaccctt gcatcctctt tagccagttc
     2821 tgaaaatagg actggtcttt ttttacgagg tgatggaata atgtctaaaa tcgaaataaa
     2881 aaatctgaca ttcggctatg acagccaagg cacattatta tttgaacaag ccaatctaaa
     2941 ctttgacaca caatggaaac taggacttat cggacgaaac ggtcgaggaa agacaacttt
     3001 actgaatatc ctgcaaaaca aactccctta tcaagggcaa gtgatccatc agcaagcgtt
     3061 tgtctatttc ccgcagcaaa caaaagataa agagcgtttg acctattatg tgttaaatga
     3121 gattatggat tttgagatat gggaaattga aagagagctc caactgatgc aaacagatcc
     3181 tgaaatctta tggagagaat tcagcacact atcaggagga gagaagacaa aagtcctact
     3241 ggcactctta tttgtggatg acactcattt cccgttaatc gatgaaccaa cgaatcattt
     3301 ggatatctct ggcagaaaac aagtagcggc ttacttgaaa aagaaaaaac aaggattcat
     3361 cgtggtgagt catgatcggg gatttatcga tgaagtagtg gaccatgttt tagcaatcga
     3421 aaagagccaa ctggaacttt atcaagggaa tttctctatc tatgaagaac agaaaaaact
     3481 tcgtgatgaa tttgaaatag ctaaaaatga aaaattgaaa aaagaagtca gcaggctaaa
     3541 gaaaacagca gctgaaaaag cagaatggtc tcgttcccga gaaggggaca aaacaaagaa
     3601 aaaagtcgga ttcatcgata cggaatctag gcgagtgaat aaaggagcag taggcgctga
     3661 tgctgcacgg acgatgaaac gatctaaagc aatcgtgaat cggatggaaa cccagatcag
     3721 tgagaaagaa aaattattaa aagatatcga gtatatcgat gctttgacga tgaatagcca
     3781 agtgtctcat cataaacggc tgttaagcgt agaaaatctt caattagggt atgaaaatct
     3841 gttattcgag ccagttcatt ttacgatcga acctcatcag cggatggcaa tttcaggtcc
     3901 taacggcgcg ggaaagtcat ccattatcca ttatcttctg gggacattca acggcaaggt
     3961 tgtaggagaa aaatcccagc ccaaacattt gaccattagt tacgtacgcc aaaattatga
     4021 agacaatcga gggacattgg cggaattcgc gaagaaaaac cgagtagact atcaagcatt
     4081 tttgaataat ctccgaaagc ttgggatgga aagagatgtt tttcataata aaatcgaaca
     4141 gatgagtatg ggacaacgga aaaaagtgga attggctaaa tctttatccc aaccagctga
     4201 gctctatata tgggatgaac cattgaatta tttggatgtc ttcaatcaag aacaattaga
     4261 acaactgatt ttgaacgtga aacctgctat gttactagtg gaacatgatc aaacctttct
     4321 agataaagta tctactgaga ttatttctct tgagaaaatc taaaccttgt aatctcaaag
     4381 acaacaaggt atggttaagg agaactttgt caacgaagga gagaatcaca tgctttatgt
     4441 aacgagacac ggagaaacaa cttggaacgc acaaggatta gtttgcgggc gagcagatgt
     4501 gccgctgact gaaaaaggac agatgcaggc acaggcacta gcagaaaagg tagtagattt
     4561 gcctgttcca atcacgaaaa tcatccattc ccctcttcaa cgagcaagag atacagcaca
     4621 agcagtagtt gatcgtctca atttgccgat gaccgctgat gagcgattgg tagaaatgga
     4681 ttttggggat tatgacggac tgccaagcaa agatgaaaac tttcaaaaag cgcgtttagc
     4741 gtttgctgta cgtttcccaa atggagagtc ggtcttagat gtgtacgcac ggatcgttcc
     4801 tttattgaaa gaatgcatgg aagacgaaga aaatgtctac ttgttagtct gtcataatgc
     4861 cttgataaga gtgatcaatg cttattttca tccaatgcct aatgaagggt tctttacatt
     4921 catggtagac aatacagaat taatttctta tgagtaatta gacttttgtg ttatttaaat
     4981 aaaatgtttc aaggcactgc ttttgcttag cagtgccttt tttgtggctg tgtcaattct
     5041 tttggcatta taaatccaat ttttagaaat aaaatttaat tattttaaca aaagtattga
     5101 aattttgttt ctttagatat atactctagt catagaaatg aagacggata cagaaaaaga
     5161 gggataatat gtttggcttt tcaatattta tgaatgattc tttgtcagaa gagaagaaac
     5221 agtatatccg ccaaatggca tccaatggct ttgtcggtat tttcacttcc atgcatatac
     5281 cggaagatga tgtttcggct tatcaaaatc gattgatcga actaggaagc gaagcgaaaa
     5341 agaataaact agagttgatg gtcgatattt caggagaggc attgaaacga gctggctttt
     5401 cattagaaga tctaacaccc ttgaaagcaa ttggtgtaac agggttaaga atggattatc
     5461 atatttctaa tcagcagatc gccgactggt cacatcaatt gaaaatcagt ttgaatgcca
     5521 gcacgatcac gcctaatgat atcgatgagt tgaaagaagc agcggctgat ttctcacaga
     5581 ttgaagcttg gcataattat tatccacgtc ttgaaacagg gttggacaaa gaatggtatc
     5641 aggaaaaaaa tcaatggctc aaaactcaag gattccttgt acaaggcttt gtaccaggag
     5701 atacagagtt gagaggtcca ctgtatcaag gattaccaac tttggaagaa catcgaggga
     5761 tccatcctct ggcaggtgct cttgatctat tagcaagtaa cacggatatt gtttatatcg
     5821 gagatgcagg tctgtcagaa cacgttcagg agcaatttgc ttctttccaa aaagaacaga
     5881 cagtcttgct ccatacggaa cctgtagatg aagtttttta cgaatatata ttaggaaaac
     5941 atacgaatcg gcaagatgat gcaagagatg tcatacgaag cgcagatgcg cgttttcgag
     6001 agattccgcc aatcccagta agaaatacag ctaagcgtat gaaaggatct attactttag
     6061 ataacgaaaa gtatttgcgc tatatgggtg agatccaatt aactaaatat gatttgccag
     6121 cggatgaaaa agtgaatgta gtagccaaag tcatcaaaga agaactaccg ctgatcaacc
     6181 aaatcaaagc tggtatgaac tatcaattta taagaaaaga aggaagataa aaatgagcaa
     6241 aatcaattta gagaacctaa caacggaacg cagaaaccaa gaaacttttg gattagatga
     6301 aatgagtatt tctcaagcag tccaactcat gaacaaagaa gatcagaaag tggccgctgc
     6361 tgttgaaaaa gaactgccaa gtatcgagcc agtgatcgcc aacacgattg aatcctttaa
     6421 aaaaggtggt cgcttgattt atatgggagc tggaacgagt ggtagacttg gtgtattaga
     6481 tgctgctgaa tgtgtaccga cattcggtgt agaaccggaa atggtcatag gcctaattgc
     6541 tggtggagaa gaagcaatga ctgtagccgt cgaaggagcc gaagattctt atgaattagg
     6601 gcaagaagat ctaatcaagc tgaatttaac aaaaaatgat atagttattg gtatcgcagc
     6661 tagcggtcgt acaccatatg tgattggtgg attagattac gcaaaatcga ttggtgctgt
     6721 tacaggaacg atttcttgca ataaacaagc agaaatcagc gagcatgcgg atttcccaat
     6781 cgaagtcgat gcaggaccag aatttttgac aggctctact cgattgaagt caggaacagc
     6841 acaaaaactg attttgaata tgatttctac tatcagtatg atcggtatcg gtaaagtatt
     6901 caataacttg atggtcgacg tcaaaccaac aaatgaaaaa ctaattgaac gctcaaaacg
     6961 aatcatcatg caagcgacag aagctgacta cgaaacagct gaaaagtact ttgaggaagc
     7021 agatcaaaat gtcaaactgg ctatcgtcat gattttgaca aatagtgata aagaggcagc
     7081 tcaagcaaaa ttagctgaag caaacggttt tgtaaaagga accttatgag ggggaaaaga
     7141 ccatggcaaa aaacgaattg actttggaag aactggcaaa acaaatttat caaggagcag
     7201 gcggtatggg caatgttgaa agcattgtcc actgcatgac tcgtgtacgt atgaaggtca
     7261 tcaatgatga tcttgtaaat gtctcggaat taaaagcgat tccaggagta cttggcgtag
     7321 tagaggatga acagctccaa gtcatcatcg gacctgggaa agtgaacaaa gtagcgcagg
     7381 aaatggttga cttagcaggc gtgaaattag gagaaaaact tccttctgct agtgcaacta
     7441 cagcaagttc ttctgcatct ggaaaagacc gtgtaaacga acgggcacaa gaaatgaaag
     7501 cggcacaaaa atcgaaacaa aaaccctcta aaatcaaatc ggtattaaaa gacatctcta
     7561 atatctttgt accaatgatc cctgcatttg tcggcactgg gatcgtggca gggattgccg
     7621 cagttttagc aaacttagtc acagctggaa cactagacgc aggaacatgg caacaatata
     7681 tcgatgtgat gaacatcttg aaaaacggga tgtttagcta tctagttatc tatacaggta
     7741 tcaatgctgc acaagttttt ggtgcaacgc caacgcttgg tggagttatc ggggctgttg
     7801 tgatgctgac agggatgaat ccagaagcac cactgaaaaa cttgtttacc ggtgaagctc
     7861 ttgcaactgg acaaggcggg atcttaggtg ttatcttcgc agtttggtta ttgtcaatcg
     7921 ttgagaaaaa actgcataaa gtagtacctg atgcggtaga tatcatcgtt acgccaactc
     7981 tttccctgat tgccatcggt ctgatcgaaa ttttcttcat catgccttta gcagggttca
     8041 tttctgacgg catggtcggt gggatcaact gggtacttgg tgttggagga gcatttgccg
     8101 gcttcgttct aggtacatta ttcttgccaa tggtcatgtt cggactacat cagatcttga
     8161 cgccaatcca tgtacaaatg attgatgaaa ctggtcgtac cttattgctt ccaatccttg
     8221 cgatggccgg agcaggacaa gtgggtgcag cattggcatt atggatccgc tgcaaaaaag
     8281 acaaagaatt aacagaaatg atcaaagggg ctttaccagt cggaatctta ggaatcggcg
     8341 aaccattgat ttacggagta acattacctc taggacgtcc atttattact gcttgtatcg
     8401 gtggaggtat cggtggggca gtgatcggaa tgatcggaaa tgtcggagca atcgctattg
     8461 gaccttcagg agccgctttg attccattga tctctgatgg taaatggtta ggctatatcc
     8521 ttggtttatt ggctgcttac gctggtggct tcgttgctac attcttcttc ggtattccta
     8581 aagaccgttt agaaaaagaa gaactagcga ccgaaacagc cacagtaaat gaagtacctg
     8641 aaaaagcagt acctcaagct tcagctgaaa gtgtgactat cttatcatca gttgctgacg
     8701 gaacagttgt ttctttagat aaagcaagtg acccagtttt tgcacaaaaa atgatgggtg
     8761 aaggttactt tgtagaacca gaaaacggac atatctattc cccggttact ggagtaataa
     8821 gttctgtttt cccaacaaaa catgctatcg gcattacgac accaaatggc ttagaaatct
     8881 tgcttcacat gggcatcaac acagttgact taggcggtac gccattcgat gtgaaagtaa
     8941 cagaaggaca gcaagtgaca cctgatacgc ttgtagcaga agctgactta gaagcaatca
     9001 aagcagcaga caaagaaacc tccatgatcg tcttggtaac caatatggat cgtgtaaaaa
     9061 actttgtctt ggaaaaaaca ggtaaagcga aagcaaaagc acctgtgatg gatgtcaaag
     9121 cttaataaga atagaaacaa tagagaacat aaaaagaaga agccgaaatt tatttttcag
     9181 cttcttcttt tgtgttattt atttttttct gccaaaattt gataaacgcg tgtcacatca
     9241 gatggtgttc cccgtaattc ataatctgcc aagaaaggaa agccgaatct ttgtgcgtat
     9301 tgtttggctg tcagacaata ttgattgttg aagtttttat taccgctacc gactacacca
     9361 aggcaaagtt tatgattgtc ttcgtattcc agatattcgc gcatcgtttc ggtcaagatt
     9421 tctgtgtcac cgttgtctaa cccattcccg ccgtctaaat aagtaggaac gaaagtaaaa
     9481 aatggttctg tttctttcgt aaaatcacta ttttcatgga tttcttttaa agtaacttca
     9541 ggattgacag gatcttctgc atgcattttt tcagcatatt ctgctagatg tttggcaaaa
     9601 gcacgcgtat ttcctgaaat cgatatatat aaaatgttca tggatcatcc cccaattctt
     9661 tccctttagt atagcatctt tttaagtttt tttggtggtg taatttggaa agggactagg
     9721 cgctttgctt gtataatgag aaaaaagtcg gtagaattat atttctatta aagtttttat
     9781 taattaagta aaaataaaaa aggtttgtta tactttttga taaaagcggt tttactttgg
     9841 aggatgctta catggataca gagcaacaga aaaaaggaat tggtttgatt ccacttgctg
     9901 ccttagtgat tggttcagcg attggaggtg gcgtatttgg tattatgacg gacatctccg
     9961 gatcggctgg tggcccagct atcttcagct gggtactcgt aggaacagca atgttgatgc
    10021 tctccttatc gatcaataat atcaataaaa aacgtccaga tttaaaaggc gggatcttta
    10081 gttacgcaga agaaggtttt ggccgtttcg ctggttttat cagcggttgg ggttactggc
    10141 tcacatgctg gttaggaaac gtggcgttcg caacattgtt gatgagtgca ttaggttatt
    10201 tcttccccgt attcgaaggt ggtcaaaatg tggcatcgat cgtgatgagt acgatcttct
    10261 tatggggata tgcatggcta gtgaatcgtg gagttgaaaa tgctagcttt gtcaatgcta
    10321 tcgtcacatt ttgtaaatta gtgccattat ttttatttat tgtagtagct atcagcacct
    10381 tttcgatcca acaattcatt aataactttt ctagcgatat catattgaat gcgaatgggc
    10441 agccgatccc gttttctagt caagtattga gttgcatcat ggtgatgctc tgggtattcg
    10501 ttggtattga aggagcttct gtcgtaggtt cacgagcaaa aaaacaatcg gatgttggga
    10561 aagcaacgat tctaggaata ttcggtttga tcattattta cgtcttggtt tccatgcttc
    10621 catatggcac aatgacgatg aatcagctgc aaacgatcaa agcgcaacct gctatgggat
    10681 atgtatttga aatgatggtt ggtaaatggg gggcggtcgt gatcaacggc ggtttgatca
    10741 tttctttagt tggggtttgg ctctcatgga caatcttgcc gattgaaaca atggggaata
    10801 tggctgagga cggacttttg cctaaagcat ggggaaaact caacaaaaat ggggcaccaa
    10861 cttatgctat cgtattgacg acgttatgta cgaatgtctt tttactttca ttattattca
    10921 ctgacagtgc atataacttt gcttatacac ttggtacagc agcgattttc ttcacgtggc
    10981 ttttcttagg gctgtaccaa atgaagcttt cttatcagcg gaaagagtgg gtccaatttt
    11041 tagtagggtt tttagcaagt gcttttcaag tatgggccat gctagttgta gcctttaatg
    11101 aagtgatgct tgttcttgtt cttttcttac caggtattta tttttacctc aaagcacgta
    11161 aagaacaaaa cgaacctgta caacatatcc acttagataa gattttactt atcggtgtga
    11221 cgatggtcgg agtaatcgca atggttttat ttgccactgg gatgcttcac gtatagaagt
    11281 ataaagagag gctgtgaagc ctgtggttta ttcaaaaaag aagcggtgac aacattcgtg
    11341 tcatcgcttc tttttttgaa taaatggtgt tcaagaagta gcttcttcta aaaataagcc
    11401 gaaatttctt gaaactggcc acttctgtca caacttcttt gttaatgaac catcatacat
    11461 tttttcgctt gaatacgagg tatcccaatg ctaagaacaa agcaatgtaa gctaaattgc
    11521 caaacagcat ttgattagta gaaagatgag tcaattgctt cataatctct tcgttgccaa
    11581 tttggttttg cagattcagc atactgattg gattccattt gatccactcc catttttgaa
    11641 tgagtgcgat gaggatacca gatatgattg atgaagcaaa ataaaagacg atccctgctg
    11701 agatggaggc accggaacta ttgataaaac aagcaatcat caacactaaa cttaaaatta
    11761 gccataaacc gatataacta cccaaagtat aagtgaataa gctttggatc agtgtctgac
    11821 cagtagaaat cttctccgta aaggaaatcg aagggaaaag gatcagtttc atcagcacgg
    11881 tgacaataat agctaaaagg tataaataaa tagaataaat aactaatgtg atccatttac
    11941 ttactaatat ttctcctcga gaatactttc gataaaggag atttttcaat gtaccatatt
    12001 gtgattccat tgaaatgatc gtacttgcag cagcaatcat gataaagaca atccaagatg
    12061 tggcactgaa aagctgagca gtcatttcaa ctggatcaat gatcgtcgtg tattttttgg
    12121 ctagaagtgc tgttcctatg agcaaagcaa ctaatagaac agataaaaca gcagttgact
    12181 ttttcttgat caatttaaat aattcttgtt ttataagcaa cgtcattttt cttcttcccc
    12241 ctttactgaa tccagaattt ccaataggga agattccaaa tcattcgtca gatgctggac
    12301 atctgtcaca tggacatttt ctgcagacaa taactggata acagttccta ataactcttc
    12361 gttttttttt agaagcttga ttttatcagg atcttccaag ggatatccag cgtctgtcaa
    12421 gatcgttcga gcagcttgat catttgtcgt agagataacg atgaaatctt gcgctgtttg
    12481 gttcaattca gccattgtac attgtttgat gatttttcct tgattgatca ggatcagctc
    12541 atcgaccatt ttttcaagct cgctgagtaa gtgactggag atcaagaaag aggtgccctg
    12601 agtagccaag tcggtaatga tttttcgcac atctcttgtt gcttgtggat ccaaaccgtt
    12661 catcggctca tctaaaataa cgatagacgg atggttcagt aaggcaagag caattcccaa
    12721 tttttgtttc attcccagcg agtaggattt cgcttttttg tgaatgtatt tttccatctt
    12781 gagctgtttc acgatagtgt cgatagcaga tacatcgttt gtttcagaga ataacttcag
    12841 atgatcataa cctgttaaga atggatagat acccggatat tcgatcaatg ctcctacttg
    12901 ttctaatcct ttatgactag agggagaaat ctcattttca ccaatacgga tcgttccttc
    12961 tgagtaatgg atcagtccta aaatagcttt catgatcgtt gtttttccag cgccattagg
    13021 acctactaat ccaacgattt tgccaggaga taagctaaaa ctgacatcgc tgagggcttg
    13081 tttgtgacca aagttcttgg ataattcttg gaccgaaaga cttttcaaaa atattcactc
    13141 cttttctata gatagtacaa gcatacacct gatgagtaga gaagtcgcta ggtcattagt
    13201 cttattttct tgttttttca tagaaagagg aatatcaaat aaaaagcccc gctttttctg
    13261 atcagtcctt gtctgcttgt ctaagtatct agtgaccgct agtgattttg atgccaatga
    13321 tgctgatgag cagcaacccg acaaagaacc aagtggaaag attcagctta tcatgaaaca
    13381 agaccacacc aataatgacc gaaccaactg cgccaatccc tgtccaaata ggataagaga
    13441 gactcatagg caaacttttg attgcctttg acaaaaagta aaaactagca atcattccaa
    13501 tcactgtgta aaaagaataa ttgattttag aaaaaccatc actccatttc atcatcgttg
    13561 accagaatac ttccaaaatt cctgctatga ttaattccat ccatgccata catacgttcc
    13621 tccttcaata taaaaaatac acaaaa
//
LOCUS       DAZKCY010000353         4803 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.guided.152, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000353 DAZKCY010000000
VERSION     DAZKCY010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.guided.152"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..787
                     /gene="vanR-A"
                     /locus_tag="ACG8XC_003040"
     CDS             92..787
                     /gene="vanR-A"
                     /locus_tag="ACG8XC_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP9192621.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            765..1919
                     /locus_tag="ACG8XC_003041"
     CDS             765..1919
                     /locus_tag="ACG8XC_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFP9192622.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2134..3102
                     /locus_tag="ACG8XC_003042"
     CDS             2134..3102
                     /locus_tag="ACG8XC_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP9192623.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3095..4126
                     /gene="vanA"
                     /locus_tag="ACG8XC_003043"
     CDS             3095..4126
                     /gene="vanA"
                     /locus_tag="ACG8XC_003043"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP9192624.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4132..4740
                     /locus_tag="ACG8XC_003044"
     CDS             4132..4740
                     /locus_tag="ACG8XC_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFP9192625.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1482 a          922 c         1119 g         1280 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc ctataatctg ataaattatt
       61 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      121 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      181 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      241 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      301 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      361 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      421 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
      481 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
      541 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
      601 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
      661 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
      721 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
      781 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
      841 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
      901 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
      961 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1021 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1081 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1141 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1201 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1261 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1321 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1381 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1441 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     1501 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     1561 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     1621 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     1681 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     1741 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     1801 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     1861 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     1921 agatgtatat aattttttag gaaaatctca aggttatctt tactttttct taggaaatta
     1981 acaatttaat attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga
     2041 gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt
     2101 tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat
     2161 ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg
     2221 gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa
     2281 tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga
     2341 gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct
     2401 gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat
     2461 tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg
     2521 gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt
     2581 ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga
     2641 tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat
     2701 gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac
     2761 tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact
     2821 gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg
     2881 ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc
     2941 caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc
     3001 acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt
     3061 aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact
     3121 gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc
     3181 taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg
     3241 gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact
     3301 ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa
     3361 ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca
     3421 aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat
     3481 ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc
     3541 cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt
     3601 tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga
     3661 attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc
     3721 tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagttgttgg
     3781 cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc
     3841 ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg
     3901 aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc
     3961 ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct
     4021 gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact
     4081 tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata
     4141 ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact
     4201 tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca
     4261 tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga
     4321 ttgcttctat gggacggtta ccgtcctaag cgtgctgtaa actgttttat gcaatgggct
     4381 gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag
     4441 atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat
     4501 cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt
     4561 atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc
     4621 agacgtttgc gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg
     4681 cactatgtat taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa
     4741 acttttaacc gttgcacgga caaacggttc tgttgcaaag ttttaaataa agaataaaat
     4801 ccc
//
LOCUS       DAZKCY010000354         4132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.guided.169, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000354 DAZKCY010000000
VERSION     DAZKCY010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.guided.169"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1560
                     /gene="msr(C)"
                     /locus_tag="ACG8XC_003045"
     CDS             82..1560
                     /gene="msr(C)"
                     /locus_tag="ACG8XC_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP9192626.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1607..2053
                     /locus_tag="ACG8XC_003046"
     CDS             1607..2053
                     /locus_tag="ACG8XC_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP9192627.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2252..2767)
                     /locus_tag="ACG8XC_003047"
     CDS             complement(2252..2767)
                     /locus_tag="ACG8XC_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9192628.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2884..4086
                     /locus_tag="ACG8XC_003048"
     CDS             2884..4086
                     /locus_tag="ACG8XC_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9192629.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1228 a          705 c          896 g         1303 t
ORIGIN      
        1 cagttttttt gcaaacagat ttttctgctc ttttttagta tcgataattg ttgttttaca
       61 tgaaatagga gtgtaaaaca tatggaaaat ttagcagtaa atataacaaa tctacaagtc
      121 agctttggca accagctaga actatctatt gattctcttc gtgtctatca gcaagatcgt
      181 atagggatca taggagaaaa tggtgtcggt aaatcgactt tgctcaaact aatagccggt
      241 gaactttttc ccgatcatgg gaaaatccaa acagagatca ccttcaacta cctgcctcaa
      301 ttaacctatc tcgctgaagc aaaggaccta aatttggaat tagccagtca tttccagtta
      361 agactggaag aaacttcaga gcggaaatgg agcggagggg aagaacgaaa gatcgagttg
      421 atacgtcttc tttcttctta tgaacaaggg atgcttctag acgagccaac aacccatcta
      481 gatagaaaaa gtattgatcg actgattgaa gagcttcgtt attattatgg tacgctggtt
      541 tttgttagtc atgatcgcta ttttctagat gagctagcat cgaaaatctg ggaagtaaaa
      601 gacggagaaa tccgagagtt ttcggggaat tatagtgcct atctcactca aaaggaattg
      661 gagaaaaaga ctcagctacg agaagcagag tcgatcatga aagagaaaaa acgattggaa
      721 aaatcgatcc aagaaaagaa aaaacaagcg gaaaagttgg aaaaagtgtc cagtaaaaag
      781 aaaaagcaac aaatcagacc ggatcggttg tcttcctcta aacaaaaaga cagtgtacaa
      841 aaagccatcc aaaagaatgc gaaaacatta gagagaagac tccaaaaaat aggagaaaca
      901 accaagccgc agcagatgaa acaaatccgt tttccagtac caaaatctct tgaactccac
      961 agccgctatc ccatcatggg acaaaatgtc caattggaaa gaagtgggag aacattactg
     1021 gtaaatggtg attttcagtt ttctttaggt aaaaaaatcg cgattgtcgg tgaaaatggt
     1081 tcaggtaaga caaccttatt agaacatatc cgcaaacaag gagaaggaat ccttctctct
     1141 ccgaaagtaa gctttcaagt atatcagcaa aagggttatc aaatgacatc tgaagaatcc
     1201 atcattcgtt ttgtcatgag acaaacagag ttttcagaat cgcttgtccg tagtttgctg
     1261 aatcacttag ggtttgctca ggaaactttg acgaaaccgt tatgtacatt aagtggggga
     1321 gaagcgaccc gtctgacgat tgctttgctt tttactaagc caagtaatgt gttgctgtta
     1381 gatgaaccga ctaattttat tgatatggca acgatcgaag ctttagagaa gctaatgcaa
     1441 atatatccgg gaacgatttt gtttacttct catgattctt actttgtcga gcgtacggct
     1501 gatgaagttt atgaaataaa agggcagaaa ataaaaaaag tacttacgag aaatttttaa
     1561 atgtgctacg atgattttcg tgacaaattt aaaggagtgg tacagattgc ctacaaataa
     1621 aaaagaagga ataattttta caacaatgat gtgttttttg atggtcttgg ggatgagcgc
     1681 gtataattta tggctgcatc aagatcttca tttatcaagt ttgttgattg gtctagtgcc
     1741 tggattcgtg gtggctttca ttttggatgt cttcatcatc ggtgtgctgg cgaaaaaaat
     1801 tgcctttaaa ctaccagtaa atccagaaag caaggtccaa atgatcttaa ctatttcatg
     1861 cttgatggtt ttaggaatgg tgacatgtat gtctttattc ggtgtgatca tggaacaagg
     1921 aatacctgat caattattct ctgcttatat gaatgcttgg aaaatgaatg tgattgccgc
     1981 tttaccgttg cagttgatca tcgttggccc gctttcaaga agtgtgctaa aagcaattca
     2041 aagtaatgaa tgatggaaca ctgttatttg aaaaaaggcc ttggcatgct ttttgtcata
     2101 gtctcttgaa tgcagttcta gaaaaatagt gatccgaacg ataggttttg gcctgtcgtt
     2161 cggatcgctt tttcttttcc aactgtattg ctcttttatt gaagataatt tgctcttcaa
     2221 tagatatttc tagaaaggtt ctgtttatcc cttactcaaa aaactgctgc atttgttctc
     2281 ttacttgctg acaccattgg agttcctgct gacaaagcat aattctgcgc tggatcacaa
     2341 cataacgtcc aaattcttgt ttcttgtcta gcgagaggtc attcaatttt ttttgatttt
     2401 tttgcatgat tttgttcagc tgttgttctc tagtgaatag cagtccagta gctgttgttt
     2461 tatcgagcgt cgaaaaagca tagatttttg ctagaaattc atctttttgc gtaggagcgg
     2521 gagtttcctc tttgatccat tcctctacaa gagcacgtcc ttcatcagtc aagtggtaga
     2581 tctttttttg agagtgctcg tctttgtcca aaacggtcac ataaccctgc tggtctaact
     2641 tggcaagcgt tgtatagatc tgactgtgat gggcttccca aaaaattgtg atcagctgct
     2701 tcaattcata cccagaacgt ggtttggata aaagcacgca taataatata taggctaaag
     2761 tattcataaa aatcctacct ctcgtcttat tttctttatt atatcacagt aaagtttcca
     2821 cgttttacat gtgcatattg acatgtaaaa taaatcatgt atgatgctaa tagagagaaa
     2881 gggatggaaa tattggagaa tcaaatagat atacaagaac aaacaaatga agaaacaaag
     2941 gggaaaagtt cagcgctagt tgtagcagct acctgtatga tcgcttttat gggagtgggt
     3001 ctagtagacc caatcttaaa aacgattgct gcacagttaa acgctacgcc agctgaaacg
     3061 accctgttat ttactagtta catgttagtc actggtgtgg tgatgctatt tacaggtttc
     3121 ttatctagtc ggatcggttt gaaaaaaaca ttgatgacag gattgtttat catcgttgta
     3181 ttcgctggtc ttggcggatt atctgggaat atcggaatgc tgattgggtt acgtgccggc
     3241 tggggtgtcg ggaatgcttt atttgtctct actgctttag caacgatcgt cagtatttta
     3301 gttggtaaca cagaaaaagc aatcatgatg tatgaagcag cacttggctg cggaatggct
     3361 gttggccctc tagtcggtgg ggtcttgggc agtggttcat ggcgttatcc attttttggg
     3421 gtagcatttt tgatgttttg cggttttata gccctagcgg tacttttacc tgaaactgag
     3481 aaaccaaaga gaaaagtagg cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt
     3541 cgttcagttg gactaatcgc attgttatat aatttcggat tctttacttt acttgcgtat
     3601 gcgccatttt tattgattgg ctattctgaa ttagaagtcg gttttgtatt ttttggctgg
     3661 ggtgtgcttc tggctattgc ttcgattttc gttgcaccgg ctcttgagcg gaagtttacg
     3721 acttatcgga caattttagc agcatttgtc ttattcatta tttgtttgct gttattagga
     3781 gctggaactt ctatgccagt ccttgtaccg atcagcattg tgttagcagg atttttccaa
     3841 ggaattatca atacactgtt gacgacgatt gctatggaaa taccaggtct gcaacgaaac
     3901 gttgcttcgt ccagctatag ttttgtccgc tttttcggtg gagctttagc accatttatt
     3961 gcaggtgtga ttggtgaaca tattggtgaa agctatgcgt tttattttgc aggtgccatt
     4021 gttcttatca gtgtatgttg tatcatttat caccgtaatt attttgctac aaatgaagaa
     4081 gaataagaaa tcagaggttg tgaaaaagct gtcctgcatc aagtcataag aa
//
LOCUS       DAZKCY010000355         3772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.guided.180, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000355 DAZKCY010000000
VERSION     DAZKCY010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.guided.180"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..1208
                     /gene="rlmN"
                     /locus_tag="ACG8XC_003049"
     CDS             141..1208
                     /gene="rlmN"
                     /locus_tag="ACG8XC_003049"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFP9192630.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            1198..1404
                     /locus_tag="ACG8XC_003050"
     CDS             1198..1404
                     /locus_tag="ACG8XC_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192631.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(1401..2504)
                     /locus_tag="ACG8XC_003051"
     CDS             complement(1401..2504)
                     /locus_tag="ACG8XC_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9192632.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3057..3605
                     /gene="aac(6')"
                     /locus_tag="ACG8XC_003052"
     CDS             3057..3605
                     /gene="aac(6')"
                     /locus_tag="ACG8XC_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP9192633.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1233 a          638 c          760 g         1141 t
ORIGIN      
        1 tttctaatac ggtatgatgt cttgtctttt tagtttttgt gagattaaag ttctattcga
       61 tttatttgtg aagtattgca ttaaggaaat gaatcatgta tagtatgtag cgaaactttg
      121 agaaaaagag ggaaaatact ttggaaaaac agtccattta tggcttgacg aatgaagaat
      181 taatcaattg gtttatagaa aacggcgaga aaaaattccg agctgcccaa gtttgggaat
      241 ggttgtatca aaaacgtgta agtaatttca cagaaatgac gaatctatcg aaacaattga
      301 tcgaaaaatt atcggagcat tttatcatca atcctctaaa acagatggtc gtccaagaag
      361 cttcggatgg tacagtaaaa tatttgtttg aactccctga taaaaacatg attgagactg
      421 tactgatgag acaagaatat ggcttgtcag tttgtgtgac gacacaagta ggatgtaata
      481 tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg actgctggag
      541 aaattgttgc acagatcatg atggtccaac attattttga tgagcgcaat cttggcgaac
      601 gcgtttcaca tgtcgttgta atggggatcg gtgagccatt tgataattac gacaatgtaa
      661 tggacttttt gcatatcatc aatgatgcca aaggattggc aattggtgct cggcacatta
      721 cagtttccac tagtggatta gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag
      781 tcaatctggc tatttccttg cacgcgccga ataatgaggt gcgaacatcg atcatgcgga
      841 tcaaccgaag tttcccaatt gagaaattga tggaagctgt ggatgagtat ttagagaaaa
      901 caaaccgtcg aatcacgttt gaatatatca tgttgaatca agtaaatgat agaccagagc
      961 atgcgcaaca attagcagat ctattaaaag ataagaaaaa attagcatat gtcaacttga
     1021 ttccatataa tccagtcagt gaacatgatc aatattctcg cagtccaaaa gctgatgtat
     1081 tgagatttta tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg
     1141 gtacagatat tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg
     1201 ccaaataatc gtatcctact cgttttttca attaccataa aaggagcagt tttcttaatt
     1261 aaggaactgt tcaacttaaa taagagaaaa gattctatag cgaaagctat aaagcttttt
     1321 ttgacaagaa aaaccctcat caagcaaaga aatgtcctca atttgatgac agcctgcttg
     1381 atgggggttt tagcaattat ttaactgatt ttcttatatc cttgctggct ttcttgtgta
     1441 gcatgataag tttctacgtc aagaatggcc tgtttaaatt tttctcgggt gatttcataa
     1501 ggcacatgtt taatatcatt tgttgccatg gctttatcta agattgcttc taaatcggtc
     1561 gtgacattga gattcatgtc ggttaacgat actggaagtt ggttcttttt cataaacaca
     1621 tagattttat caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa
     1681 ctgacagatg ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag
     1741 aatgagtgtg ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta
     1801 gtaacaatga tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta
     1861 tctttcaatg cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg
     1921 attcctaatc tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact
     1981 tctttggata aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata
     2041 attgctgtat caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt
     2101 acgccttctt cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata
     2161 gtgggaaagg tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa
     2221 gcacggccac caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc
     2281 aactgttcaa tatttccata agttgaatta cccccgtacc agagactggc gacaacctct
     2341 atttcctgtg tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct
     2401 ccaatcagca ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca
     2461 tctgttccaa tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt
     2521 cttcgttgaa acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga
     2581 gtagaggtga acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc
     2641 atcaataagt atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt
     2701 tttgttcctc ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc
     2761 taaaaattaa gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt
     2821 ttaaagcaaa agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg
     2881 gctttcctga tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa
     2941 gtaagataaa taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga
     3001 acatttcgtt ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga
     3061 taatcagtga atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga
     3121 gactgacttg gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga
     3181 atccagaacg aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg
     3241 caatccctca atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc
     3301 gacgaaagaa ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca
     3361 gaggaggaat cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc
     3421 aaacggacct gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac
     3481 atccgtatga attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa
     3541 atggctggga caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc
     3601 aataataaaa agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg
     3661 aaaattactt cttgaatttt tgtgaaattc caacttattt ccgaagaagc tacttttgga
     3721 acaccgtgta ccagaggttg tgaaaaagtc gcttagtttc gagtagtaag aa
//
LOCUS       DAZKCY010000356         3417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.guided.188, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000356 DAZKCY010000000
VERSION     DAZKCY010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.guided.188"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..521
                     /gene="dfrG"
                     /locus_tag="ACG8XC_003053"
     CDS             24..521
                     /gene="dfrG"
                     /locus_tag="ACG8XC_003053"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP9192634.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(593..2545)
                     /locus_tag="ACG8XC_003054"
     CDS             complement(593..2545)
                     /locus_tag="ACG8XC_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP9192635.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2552..2788)
                     /locus_tag="ACG8XC_003055"
     CDS             complement(2552..2788)
                     /locus_tag="ACG8XC_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192636.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3026..3417)
                     /locus_tag="ACG8XC_003056"
                     /pseudo
     CDS             complement(3026..3417)
                     /locus_tag="ACG8XC_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
BASE COUNT         1024 a          619 c          557 g         1217 t
ORIGIN      
        1 tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg gataagaata
       61 gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg gaatatgtta
      121 aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa tcaatcggaa
      181 gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt acctttaatg
      241 gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac gaagaagaaa
      301 tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt gagaaaatgt
      361 acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa gtgaattatg
      421 aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat ccgtataact
      481 actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt taattgaaga
      541 agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt tgtcatttga
      601 aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt tgagcaggtg
      661 tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa gttcttagaa
      721 ttgtaatcgc aaattgagca tacttcggat tgaagttagc gtaaatatag ctcttcttat
      781 caccacgagc tgttgttaat ggtctttcaa gagatgatat ataacgtctt atctgttgaa
      841 taaagctatt ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt tcgtttggtt
      901 ccagagcaga taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc ttagtggcta
      961 atggatgttt gatagggtta tcggcatatt tgcgatatct ttctccatct ttactaatag
     1021 cctcttcgtg gaattgatga cttgttagta attcagttag ataatgagat gccaaagtcc
     1081 gtagaaactt tgttttgaat cctctgatat ctccccaatc gagcagttct gcttttgctt
     1141 gtccgaattc ttttaagcat tgttttctgc ttttcgtctt gttaacttgg caaataaaat
     1201 gatgtgcatc gctcaacctt aattctttcg tgaatactct ataaaaggcg tttctaatag
     1261 agaagtcatc atcactaatc attcgccatt cgtcggcatt aaccatttgt ttaattaacc
     1321 aatagtgagc agttgtagta taaggagcat caacatttaa accatcgaca aatctactcc
     1381 tattagtaat tttccctaat tcgtgaagat aagcattcct attttcgcta tcgttagcgg
     1441 atggttcttg tggataataa gaccaatcta acctatcatt tttacgacta aaagtattga
     1501 gatggtcttc tttgaatttt cttgtatctt catttaattc atccattgaa atattccaat
     1561 cataggcaac atcagaacgg aacacatatc ttgataagac ctcagcagat acaactatat
     1621 acgtttgcat attaaggtct tcaaaccccg tatatttctt tgaaccttgt cctttcttac
     1681 ggacgttatt taggtagtag tgcatcttgt ccgtattaag ccacatctcg ttaaatttct
     1741 ttgtttgaag gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc ctatacagcc
     1801 actccagctt atgatagtag gttccaactc caattcctaa aatatcacaa gtacgactga
     1861 ctgaaaccct tccaacaacc atcttagcga acattggaag aatggtgttc ttttgttggt
     1921 gataagtagt ggtttcttct ctttttggta gaacattagt gaacttctta cacgccttac
     1981 attgatagcg ttgagatttg cccctacttt tccccctttt gtaaaattgc ttcggctcat
     2041 tgaatggggt tgattcttct tccgagcagc cttctttatg aaattgataa tctggctcaa
     2101 catcttggat gctattgatt tctataaggc gtttaatttc ttcaacaaca gaccaattag
     2161 ataaaggggt tactgtacag ttttgcgata cacctctgcc aataggattg tcattgcagt
     2221 acataccttt atgaccttta tctttggaac taccagtcat tttatatcga cttggtttcc
     2281 cttttacatc tttgaatttg tgctgttctt tcccaaaatt acaacaaaac ggattgatgc
     2341 agtaattata ttgaatggga tattcagaac cgttccattt aaaagacgtt ggcaagaaca
     2401 acattttgtt ataccttgtt gcaaatcgtt ttggagttaa taaatcatat tgtttagccc
     2461 tatcttttaa ttcttcgttt gaaatcggaa ttttaacatc tacaatttca tcttccttcg
     2521 tggctaatct tttaagcttg gtcattgtct atcaacccac tctatctttg atacccattt
     2581 tttcaacaat ccgcttatta cttcgcttgt tggcaatcca ttcaataaac ttcttatcgt
     2641 ctaaaatgtt caataagaat tcatccactg cttcgctttc agtacgctct gcatattcag
     2701 cgtattgctt aactatcacc cttacttgct ctgatatttc ccaatccaca gacttagcgt
     2761 tcttattttt tggctcaaga aattgcatag gaaaatgcac ccctttttgc ttagtaaatt
     2821 gtaaccattt taacacacca atttgcattc ctacaataat tatttcacgt tcgcattttt
     2881 atatacattt taacttacat tttgtggtgt aaattaacaa caaacgttga tttaacaata
     2941 aaaaacgagt atcaaattca gttgcaattt gatactcatt taggtttaca ttttcacttt
     3001 ttgtactcca aaactgaact acttaatatg caagacgttg tctttttttt gtatattcaa
     3061 ttttgaaaac atttccgcaa agttcagacg gacctcgatg tgttgatgaa taaaatttat
     3121 tagtaataac ttctataagg ttatcaatcc ctatattact ttttgcactt ccatgataaa
     3181 gagggaacag agaacaattc tgaaatctta tgctttcctc ttgttcgagt tccaatgctt
     3241 ctaatgattt accggacata tatttctcta aaaggtcatc gtttccctct attaccgtat
     3301 cccattgttc agattcggta aagttcgtca cacacatatt aggatacagt tctaccttct
     3361 gtttgattac aatttcggca gaaagtttct ctttaatatc ctgataaacc gttgata
//
LOCUS       DAZKCY010000357         2400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.guided.216, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000357 DAZKCY010000000
VERSION     DAZKCY010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.guided.216"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..956
                     /locus_tag="ACG8XC_003057"
     CDS             48..956
                     /locus_tag="ACG8XC_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFP9192637.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            953..1495
                     /gene="sat4"
                     /locus_tag="ACG8XC_003058"
     CDS             953..1495
                     /gene="sat4"
                     /locus_tag="ACG8XC_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFP9192638.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            1588..2382
                     /locus_tag="ACG8XC_003059"
                     /note="aph(3')-IIIa"
     CDS             1588..2382
                     /locus_tag="ACG8XC_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFP9192639.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRAIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          818 a          366 c          570 g          646 t
ORIGIN      
        1 aaaaagaaga tgtaataata tagaaaaaat aaacgaggag tatgtaaatg agatcagaaa
       61 aagaaatgat ggatttagta ctttctttag cagaacagga tgaacgtatt cgaattgtga
      121 cccttgaggg gtcacgcgca aatattaata tacctaaaga tgaatttcag gattatgata
      181 ttacatattt tgtaagtgat atagaaccgt ttatatctaa tgatgactgg cttaatcaat
      241 ttgggaatat aataatgatg caaaagccgg aggatatgga attattccca cctgaagaaa
      301 agggattttc ctatcttatg ctatttgatg attacaataa aattgatctt accttattgc
      361 ccttggaaga gttagataat tacctaaagg gcgataaatt aataaaggtt ctaattgata
      421 aagattgtag aattaaaagg gacatagttc cgactgatat agattatcat gtaagaaagc
      481 caagcgcaag ggagtatgat gattgctgca atgaattttg gaatgtaaca ccttatgtta
      541 ttaaaggatt gtgccgtaag gaaattttat ttgctattga tcattttaat cagattgttc
      601 gccatgagct gctgagaatg atatcatgga aggtcggcat cgaaacaggc tttaaattaa
      661 gtgtaggcaa gaactataag tttattgaaa ggtatatatc cgaggatttg tgggagaaac
      721 ttttgtccac ctaccggatg gattcctatg aaaacatatg ggaagcatta tttctatgcc
      781 atcaattgtt cagggcggta tccggtgagg tggcggaaag gcttcattat gcctatccgg
      841 agtatgatag gaatataaca aaatatacca gggacatgta taaaaaatac actggtaaaa
      901 ccggctgcct ggatagcaca tatgccgctg atatagaaga gaggcgggaa cagtgattac
      961 agaaatgaaa gcagggcacc tgaaagatat cgataaaccc agcgaaccat ttgaggtgat
     1021 aggtaagatt ataccgaggt atgaaaacga gaattggacc tttacagaat tactctatga
     1081 agcgccatat ttaaaaagct accaagacga agaggatgaa gaggatgagg aggcagattg
     1141 ccttgaatat attgacaata ctgataagat aatatatctt tactaccaag acgataaatg
     1201 cgtcggaaaa gttaaactgc gaaaaaattg gaaccggtac gcttatatag aagatatcgc
     1261 cgtatgtaag gatttcaggg ggcaaggcat aggcagcgcg cttatcaata tatctataga
     1321 atgggcaaag cataaaaact tgcatggact aatgcttgaa acccaggaca ataaccttat
     1381 agcttgtaaa ttctatcata attgtggttt caaaatcggc tccgtcgata ctatgttata
     1441 cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc
     1501 aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata
     1561 ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa
     1621 aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta
     1681 tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa
     1741 gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg
     1801 cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt
     1861 gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt
     1921 atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc
     1981 tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg
     2041 gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg
     2101 tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg
     2161 ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga
     2221 agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccgggcgat cagggaggat
     2281 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
     2341 gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga tttagatgtc
//
LOCUS       DAZKCY010000358         2400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.guided.217, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000358 DAZKCY010000000
VERSION     DAZKCY010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.guided.217"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..956
                     /locus_tag="ACG8XC_003060"
     CDS             48..956
                     /locus_tag="ACG8XC_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFP9192640.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            953..1495
                     /gene="sat4"
                     /locus_tag="ACG8XC_003061"
     CDS             953..1495
                     /gene="sat4"
                     /locus_tag="ACG8XC_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFP9192641.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            1588..2382
                     /locus_tag="ACG8XC_003062"
                     /note="aph(3')-IIIa"
     CDS             1588..2382
                     /locus_tag="ACG8XC_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFP9192642.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          818 a          366 c          569 g          647 t
ORIGIN      
        1 aaaaagaaga tgtaataata tagaaaaaat aaacgaggag tatgtaaatg agatcagaaa
       61 aagaaatgat ggatttagta ctttctttag cagaacagga tgaacgtatt cgaattgtga
      121 cccttgaggg gtcacgcgca aatattaata tacctaaaga tgaatttcag gattatgata
      181 ttacatattt tgtaagtgat atagaaccgt ttatatctaa tgatgactgg cttaatcaat
      241 ttgggaatat aataatgatg caaaagccgg aggatatgga attattccca cctgaagaaa
      301 agggattttc ctatcttatg ctatttgatg attacaataa aattgatctt accttattgc
      361 ccttggaaga gttagataat tacctaaagg gcgataaatt aataaaggtt ctaattgata
      421 aagattgtag aattaaaagg gacatagttc cgactgatat agattatcat gtaagaaagc
      481 caagcgcaag ggagtatgat gattgctgca atgaattttg gaatgtaaca ccttatgtta
      541 ttaaaggatt gtgccgtaag gaaattttat ttgctattga tcattttaat cagattgttc
      601 gccatgagct gctgagaatg atatcatgga aggtcggcat cgaaacaggc tttaaattaa
      661 gtgtaggcaa gaactataag tttattgaaa ggtatatatc cgaggatttg tgggagaaac
      721 ttttgtccac ctaccggatg gattcctatg aaaacatatg ggaagcatta tttctatgcc
      781 atcaattgtt cagggcggta tccggtgagg tggcggaaag gcttcattat gcctatccgg
      841 agtatgatag gaatataaca aaatatacca gggacatgta taaaaaatac actggtaaaa
      901 ccggctgcct ggatagcaca tatgccgctg atatagaaga gaggcgggaa cagtgattac
      961 agaaatgaaa gcagggcacc tgaaagatat cgataaaccc agcgaaccat ttgaggtgat
     1021 aggtaagatt ataccgaggt atgaaaacga gaattggacc tttacagaat tactctatga
     1081 agcgccatat ttaaaaagct accaagacga agaggatgaa gaggatgagg aggcagattg
     1141 ccttgaatat attgacaata ctgataagat aatatatctt tactaccaag acgataaatg
     1201 cgtcggaaaa gttaaactgc gaaaaaattg gaaccggtac gcttatatag aagatatcgc
     1261 cgtatgtaag gatttcaggg ggcaaggcat aggcagcgcg cttatcaata tatctataga
     1321 atgggcaaag cataaaaact tgcatggact aatgcttgaa acccaggaca ataaccttat
     1381 agcttgtaaa ttctatcata attgtggttt caaaatcggc tccgtcgata ctatgttata
     1441 cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc
     1501 aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata
     1561 ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa
     1621 aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta
     1681 tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa
     1741 gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg
     1801 cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt
     1861 gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt
     1921 atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc
     1981 tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg
     2041 gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg
     2101 tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg
     2161 ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga
     2221 agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat
     2281 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
     2341 gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga tttagatgtc
//
LOCUS       DAZKCY010000359         2369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.guided.222, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000359 DAZKCY010000000
VERSION     DAZKCY010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.guided.222"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8XC_003063"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8XC_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFP9192643.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKINIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     HPNMRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="ACG8XC_003064"
     CDS             2039..2227
                     /locus_tag="ACG8XC_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFP9192644.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..>2369)
                     /locus_tag="ACG8XC_003065"
     CDS             complement(2285..>2369)
                     /locus_tag="ACG8XC_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9192645.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          818 a          359 c          463 g          729 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acagaggtac aacgaaaacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacggcgat aacctctttt cagtggaaaa atactaagat aaacatcata
      241 gacacgccag gacatatgga ttttttagca gaagtatatc gttcattatc agtattagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattgttt
      361 catgcactta ggaaaatagg tattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc ggaaattgta
      481 atcaaacaga aggtagaact gcatcctaat atgcgtgtaa tgaactttac cgaatctgaa
      541 caatgggata tggtaataga aggaaatgat taccttttgg agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt
      961 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatg
//
LOCUS       DAZKCY010000360         2001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.guided.237, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000360 DAZKCY010000000
VERSION     DAZKCY010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.guided.237"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8XC_003066"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8XC_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFP9192646.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKINIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     HPNMRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          680 a          313 c          411 g          597 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acagaggtac aacgaaaacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacggcgat aacctctttt cagtggaaaa atactaagat aaacatcata
      241 gacacgccag gacatatgga ttttttagca gaagtatatc gttcattatc agtattagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattgttt
      361 catgcactta ggaaaatagg tattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc ggaaattgta
      481 atcaaacaga aggtagaact gcatcctaat atgcgtgtaa tgaactttac cgaatctgaa
      541 caatgggata tggtaataga aggaaatgat taccttttgg agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc agaatgagtt tttgaagtta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatcccctc cctctgctgc aaacgactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctgcgat attatgtgga ttctgcgaca catgaaatca tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgacttg tgctctgctg caagaaaagt atcatgtgga gatagaaata
     1261 aaagagccta cagtcattta tatggaaaga ccgttaaaaa aagcagagta taccattcac
     1321 atcgaagttc caccgaatcc tttctgggct tccattggtc tatctgtagc accgcttcca
     1381 ttagggagcg gagtacagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     1441 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     1501 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta a
//
LOCUS       DAZKCY010000361         1893 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.guided.242, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000361 DAZKCY010000000
VERSION     DAZKCY010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.guided.242"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            260..1171
                     /locus_tag="ACG8XC_003067"
     CDS             260..1171
                     /locus_tag="ACG8XC_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFP9192647.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG8XC_003068"
     CDS             1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG8XC_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFP9192648.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          668 a          263 c          362 g          600 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaa
//
LOCUS       DAZKCY010000362         1769 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.guided.248, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000362 DAZKCY010000000
VERSION     DAZKCY010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.guided.248"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..348
                     /locus_tag="ACG8XC_003069"
                     /pseudo
     CDS             1..348
                     /locus_tag="ACG8XC_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(620..910)
                     /locus_tag="ACG8XC_003070"
     CDS             complement(620..910)
                     /locus_tag="ACG8XC_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9192649.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1118..1769)
                     /locus_tag="ACG8XC_003071"
                     /pseudo
     CDS             complement(1118..1769)
                     /locus_tag="ACG8XC_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321659.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          469 a          339 c          281 g          680 t
ORIGIN      
        1 gttaaatacc ttatttgatg caaaagaaat ctacgatagt caacaaaaaa attttcatta
       61 tatccagaaa aatttttctt agttaaggac ctatggattg cagtaatgca gaattcgtcg
      121 aataaactgc caaagacata caatcatatt gcttttaaaa ttgacgaaca agatatagat
      181 tcgtttgtct caaaaatcca aatgttaggt ttaactgtcg aaccaggacg ttctcgtgtg
      241 aaaggcgagg cttcttcaat ttacttttac gactatgata atcacctctt cgagttacat
      301 accggtactc tccaggaacg tttaaaatcc tacaattcca ctacctgaga ctagtaatga
      361 ctagtctctt tttatggcct ctgatccccc cattgagcga atactaatag ggcttcgaat
      421 tgatctgttc aatctttaag caatatccct caaatgagtt tggaaagttt agtgattcat
      481 cacaaatact taaatacatg tagttatata tagattgagg ttcttgtgag cgtatgctcg
      541 tgtgggttca aatcccatcg tctgcatcca tctttccaaa acaagcggct aggacatttt
      601 tgtctcagcc gcttgttttc tagtttattc tttcatcttg tcggaccttt tttcttgggt
      661 atagactttt ctcatattct accacaattt tgggtttccc taacacatga taggatctca
      721 caacaaattg ctttctatta ttataataac ctgtaacgac taacttcatc ccttcgtcta
      781 cttcagctag gaagttcaaa ctatgggaag caatcaaaca attgacccca tttaaactga
      841 aacggatcaa cggtctggca ctaacttgca aagtacggat cttatctact gttccatatt
      901 ttttctccat aaccttcacc tcaataccat tatacgaaca tacattccct ttgtaaagaa
      961 gaaactctgt tttatctttt tgactttttg tctattacct ttcttaaaat catattggca
     1021 cttcctgcga agatgaagga catgtactaa aaaaaagagg cagcagtata gattactgtt
     1081 gcttctttac taatttaggt ttacttgtta agaaagatta tttacgcaaa tgttcttcaa
     1141 caatttgctg agatttatca gctgtatctt ccacttcatc ttttgctttt tctaactttt
     1201 gaatatattg tttcaagctt gagttttcag cagttgcttc gtctaatttc ttttgtgtat
     1261 ctgttaactg atttctacat ttgttttttc ttgtgtcagt gttgcgattt gttgattcaa
     1321 accatttaca gtattttgta attctgaaag ttttgcttgt gcttcttgta gtcttgctgt
     1381 ccaatctgca ttcagatgat tgatttcatc atttttttgg ttgatctcat ctagtttgac
     1441 attgatttca ttttgtttgc tttgtagttg tgcatccttg ctgactaatt gactttctaa
     1501 gttttgtttt tctgctgccg attgattttt ttggttttct aattcattta cttttgtttg
     1561 gtattcttgg acatctgcct ttgaatcttg caatgtctgt tctttttgtg ctagtcgagc
     1621 ggtaagatct gtaatcgctt gctctttatc tgtcaatgta tgatcaagct gtgtcagatt
     1681 ctcattgatt gtatcaatat ctgtctctcc accccacaaa ataatttcat ttgcgtaccg
     1741 cgtgcctgtg tagccaatta gacatagaa
//
LOCUS       DAZKCY010000363         1210 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.guided.283, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000363 DAZKCY010000000
VERSION     DAZKCY010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.guided.283"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..807
                     /gene="erm(B)"
                     /locus_tag="ACG8XC_003072"
     CDS             70..807
                     /gene="erm(B)"
                     /locus_tag="ACG8XC_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFP9192650.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            977..1210
                     /locus_tag="ACG8XC_003073"
                     /pseudo
     CDS             977..1210
                     /locus_tag="ACG8XC_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          459 a          202 c          205 g          344 t
ORIGIN      
        1 ctaaaattac aaacaaatcg tttaacttct gtatttattt atagatgtaa tcacttcagg
       61 agtgattaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag tgaaaaagta
      121 ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta cgaaattgga
      181 acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt aacgtctatt
      241 gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa cactcgtgtc
      301 actttaattc accaagatat tctacagttt caattcccta acaaacagag gtataaaatt
      361 gttgggaata ttccttacca tttaagcaca caaattatta aaaaagtggt ttttgaaagc
      421 catgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac cttggatatt
      481 caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt gcttaagctg
      541 ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg tcttaataaa acttacccgc
      601 cataccacag atgttccaga taaatattgg aagctatata cgtactttgt ttcaaaatgg
      661 gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc aatgaaacac
      721 gccaaagtaa acaatttaag taccgttact tatgagcaag tattgtctat ttttaatagt
      781 tatctattat ttaacgggag gaaataattc tatgagtcgc ttttttaaat ttggaaagtt
      841 acacgttact aaagggaatg gagataaatt attagatata ctactgacag cttccaagaa
      901 gctaaagagg tccctagcgc ctacggggaa tttgtatcga taaggggtac aaattcccac
      961 taagcgctcg ggaccccttg taggaaaatg tcctaagtgt ggcaacaata ttgtattaaa
     1021 aaaatcgttt tatggttgtt caaattatcc tgaatgtaag tttactttag ctgaacattt
     1081 tagaaagaaa aaactaacca aaacgaatgt aaaagaatta ctggagggaa aagaaaccct
     1141 ggtaaaagga atcaaaaaca aagagaaaaa gccctacaat gccgttgtaa aaattgggga
     1201 aaagggatat
//
LOCUS       DAZKCY010000364          572 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4114
            SAMEA115707015-rid22580013.guided.332, whole genome shotgun
            sequence.
ACCESSION   DAZKCY010000364 DAZKCY010000000
VERSION     DAZKCY010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707015
            Sequence Read Archive: ERR13196747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287853.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287853.1
            Genome Coverage       :: 124x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:46:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,915
            CDSs (with protein)               :: 2,915
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707015-rid22580013.guided.332"
                     /isolate="VRE4114"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>572
                     /locus_tag="ACG8XC_003074"
     CDS             <1..>572
                     /locus_tag="ACG8XC_003074"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9192651.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKGDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNN"
BASE COUNT          227 a           53 c          105 g          187 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaaggaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tg
//
